<?xml version="1.0" encoding="UTF-8"?>
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<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KLN5</accession>
    <name>A0A3B5KLN5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KLN5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001023"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001036.1">
        <property type="protein sequence ID" value="ENSXCOP00000001023.1"/>
        <property type="gene ID" value="ENSXCOG00000000851.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158277"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000093">
        <property type="entry name" value="Protocadherin 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000248">
        <property type="entry name" value="Protocadherin 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000018">
        <property type="entry name" value="Protocadherin gamma c3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF348">
        <property type="entry name" value="PROTOCADHERIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017177525" description="Cadherin domain-containing protein" evidence="13">
        <location>
            <begin position="17"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="680"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="14"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="121"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="230"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="338"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="443"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="566"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="804"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="860"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="821"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="871"/>
            <end position="888"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="962" mass="105462" checksum="0D549CAB82282CC6" modified="2018-12-05" version="1">MIVVLIALCFTDGALSQIRYSVPEEAEHGTLVGNIAEDLGLDLTKLASRRFQVVPSSRTPYLEVNLENGVLFVNEKIDREQICKQSASCQLNMEVFLENPLELFRVEIEVVDINDNPPSFPETDITVEISESATPGTRFPLESAFDPDVGSNALRTYDITTNNYFYLDVQTQTDGNKFAELVLEKPLDREQQAAHRYVLTAVDGGQPPRTGTALLVVKVLDSNDNVPVFEQPVYTVSLSENVPVGTLVIQLNATDLDEGLNGEIVYSFSNHISSRVKELFGIDPRTGRIEVRGEVDFEESSLYQIFVQAKDLGPNAVPAHCKVLVKVADVNDNAPEITFSTVTESVSEKAAPGTVIALLSVTDRDAEENGQIHVEILGDVPFKLKSSFRNYFTIVTDGLLNREQADSYSVTVVARDKGTPSLASSKSIRVQVSDENDNAPTFTQSVYDVYVTENNVPGAYIHAVTALDHDIGQNALITYSILECDIQGMSVKTYVSINEETGYLYALRSFDYEQLKDFTFMVRAKDSGAPELSSNATVKVIIVDQNDNAPLVLAPLGKNGTVKEPLPRSAEPGYLVTRVVAMDADDGENARLSYSIQRGNDNGMFRMDWRTGELRTARRVSAKRDPHQQYDLVIEVRDHGQPPLSSSASILVMLVDSMAEGRGVGDKVGTSKAKDGALDLTLILIIALGSVSFIFLLVMIVLAVRCQKDKKLNIYTCLTSDCCLGCSSCCSRQGRSRKKKLSKADIMLVQSTVNVSGPGTAQVPVEESGSFGSHHQNQNYCYQVCLTPESAKTDLMFLKPCSPSRSTDTDHNPCGAIGSGYTDQQPDIISNGSILSNETKHQRTELSYLVERPRRVNSSAFQEADLVSSKDSGHGDSEQGDSDHDATNRGHSSGKISVPHLFLQPFYLNVLCWKLKEQMSCSQGMNFSGGCAQQLCLTGAIVDVVQFDALWRSKKQKQKTAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KMD7</accession>
    <name>A0A3B5KMD7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KMD7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001273"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001292.1">
        <property type="protein sequence ID" value="ENSXCOP00000001273.1"/>
        <property type="gene ID" value="ENSXCOG00000001047.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000246762"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="78" mass="8941" checksum="F18DF04128CE0E2D" modified="2018-12-05" version="1">MEERSFPNQYPDWDQGFAVSNAGAELDSDEKVQSKIKDLLQQMEEGLKTADPHDFSTYTGWTGLCQMKKWCSKLFCLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KPN0</accession>
    <name>A0A3B5KPN0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sulfatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KPN0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002093"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002123.1">
        <property type="protein sequence ID" value="ENSXCOP00000002093.1"/>
        <property type="gene ID" value="ENSXCOG00000001603.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161140"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.10:FF:000001">
        <property type="entry name" value="Arylsulfatase E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000233">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.550">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050738">
        <property type="entry name" value="Sulfatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693:SF5">
        <property type="entry name" value="ARYLSULFATASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42693">
        <property type="entry name" value="ARYLSULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14707">
        <property type="entry name" value="Sulfatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00523">
        <property type="entry name" value="SULFATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017185924" description="Sulfatase N-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase N-terminal" evidence="8">
        <location>
            <begin position="26"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="587" mass="65680" checksum="D3995ED562F6C5D1" modified="2018-12-05" version="1">MLAPLLLSLLLASGSDVKGEVEDKKPNFVLMMVDDLGIGDIGCYGNDTIRTPNIDRLASEGVKLTQYIAAAPLCTPSRAAVMTGRYPLRSGMAGTGRVQVVLFLGGSGGLPPNETTFAKRLQKQGYTTGLVGKWHLGVNCESRGDHCHHPNQHGFSYFYGLPFTLFNDCVPGEGSDVLVDLRLALQHLTLLLGLGLLTMVGVTLSWFYFSFPTFIRNRYVAAAVWYVPFGFLQTWNCIIMRNQDVIEQPMTVETLPQRLLAEAQNFIKRNADHPFLLFFSLAHIHTPLFTTPAFAGKSRHGRYGDNLEEVDWIIGKITETVDSLGLANNTFMHFTSDHGGHLEDSDSRIGQKGGWNSIYKGGKAMGGWEGGIRVPGIFRWPGRLAAGRVVDEPISLMDLYPTLKYLAKDTQPDRHLDGYNLMPLLEGKAARSKHEFMFHYCGGYLNAVRWHPTDSNSIFKVHFFTPNFFPPGAGGCYDTKVCLCHGQHVTHHNPPLLYDIFHDPSESRQLTPETEPRYAEILQQIAKAVEKHKSTFPSKEGFDDVQVQTNTIQNQMSWEKILWKPWLQPCCGTFPFCGCKEDKNIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQV9</accession>
    <name>A0A3B5KQV9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQV9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000272.1">
        <property type="protein sequence ID" value="ENSXCOP00000000265.1"/>
        <property type="gene ID" value="ENSXCOG00000000254.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286918"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000414">
        <property type="entry name" value="Zinc finger protein 398"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF178">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="125"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="168" mass="19270" checksum="E5033CF311551B27" modified="2018-12-05" version="1">MDVTDRSVENLSQEPVGAVAKEKSVWKKIRHCLGLRKPQMFKKKSKKASETDTSKQEKHKKAHAGQKLYSCKICDKTFSRNDILLRHRRIHKDERPFSCGTCGKSYSKRGTLTHHMRIHTGEKPFSCGSCGRGFSLKTHLNAHMKVHTGEKRHSNYSLQPKKIDLDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQZ5</accession>
    <name>A0A3B5KQZ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RAD50-interacting protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">RAD50 interactor 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RINT1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027900034.1">
        <property type="nucleotide sequence ID" value="XM_028044233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQZ5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002543"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002578.1">
        <property type="protein sequence ID" value="ENSXCOP00000002543.1"/>
        <property type="gene ID" value="ENSXCOG00000002027.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114161128"/>
    <dbReference type="GeneTree" id="ENSGT00390000017006"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070939">
        <property type="term" value="C:Dsl1/NZR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060628">
        <property type="term" value="P:regulation of ER to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.670:FF:000003">
        <property type="entry name" value="RAD50-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.670">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042044">
        <property type="entry name" value="EXOC6PINT-1/Sec15/Tip20_C_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007528">
        <property type="entry name" value="RINT1_Tip20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13520:SF0">
        <property type="entry name" value="RAD50-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13520">
        <property type="entry name" value="RAD50-INTERACTING PROTEIN 1 RINT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04437">
        <property type="entry name" value="RINT1_TIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51386">
        <property type="entry name" value="RINT1_TIP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00061158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00073943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00079551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="789" mass="88985" checksum="15E4668A13C70FFA" modified="2018-12-05" version="1">MAAVTENQQNSPMRKSSDQLKEKVSHRNGGVPGDLGEQEDYGLSGVLEKEQSDILKSSRNVCELLEKIREETEALEKQVVNVCSSGPPKVAAALSAAVEVRYSLDELLQREQRISNRLQQHLQGTKQWMEDLGQTCNHVDFIERSMKYLKCLHHIEELSAAVQQSLMINSVWDAIKGVENMAALDCGLNQSQCSHLCDFLRKTLCFWHNIIKDRLTSDFEKVLAQLQWPIITPNQSVTTPANYQELSSQLEGLLLQLLALQTSDDLISQRVSASTQGAPPGSSSTLSPAAPSLCLPIQIMLQPLSKRFRYHFYGNRQTNSIHKPEWYLTQVLKWMENSATFMEEKIQPVFDRSGQTISAKVALCQGLVCLVQEKVASDASRMLYDDGLFCHLVEEVLQFEKDLRSHHSYPAMLPALLHILLDDAFLQKWLAVERKMAVEKMDAMLSAEGAWTSQYKDISDMDELKAPECAETFMTLLQVITERYRALPSPAAQLRFFGLQKELVDDFRIRLTQVMKEESRCPLGVRYCAILNAVNYISTILRDWGDNVFFLQLQQAAVSLGQQEVLGSLEMTEVGRLASLEGSLFDGLLSLLDRLKGDMLGRLLHFLMRDTAEKAQPYCQERWLSIPPPQDQSAMSLSSSACPMMLCVRDRLLNLHQVLSVSLFQLAWQGLAEKLDNFIYQEVILSNHFSDGGAAQLQFDMTRNLFPLFGHYCRRPENFFKHVKEACIILCLKVGSAILLKDLLKRSEQEATDFEDPSPESALNELGVFCLAPCDVLILLNLKALLPGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KV99</accession>
    <name>A0A3B5KV99_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">MYB proto-oncogene like 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KV99"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004053"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004097.1">
        <property type="protein sequence ID" value="ENSXCOP00000004053.1"/>
        <property type="gene ID" value="ENSXCOG00000003181.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156091"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1070">
        <property type="entry name" value="Zinc finger, BED domain-containing"/>
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        <property type="entry name" value="HATC_C_dom"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
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        <property type="entry name" value="Myb_dom"/>
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        <property type="entry name" value="MYB_TF"/>
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    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
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        <property type="entry name" value="MYB PROTEIN-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF30">
        <property type="entry name" value="MYB-RELATED PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05699">
        <property type="entry name" value="Dimer_Tnp_hAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140996">
        <property type="entry name" value="Hermes dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="29"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="29"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="74"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="74"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="126"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="9">
        <location>
            <begin position="131"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="203"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="774" mass="87441" checksum="0E4A0716E0D1DA77" modified="2018-12-05" version="1">MEEEGDSKSSFVTSLDRVEKKNDVECKIKWTQEEDENLKILVTNFGKKDWKTIASFLPGRTELQCILRWNKHLDPELVKGPWSKEEDEKILELVDKYGTKNWTTIAKHLNGRQGKQCRERWVNHLDPLITKGIWSAEEDLIIYKAHCILGNRWAEISKLLPGRSENAIKNHWNGTIRRKADMGFYKGVSETISIDIQHFTDGEKESDKVKPKAAVKTQPTSAPRRDVSSPCPSLSRSSSSSPSSVVAPSATQVDQKKFVKAALRMIAEDMLPLSFVEGAGFRSLMGTICPEYNKLSQRIIGLQLYEDVERTIKPQLIRDLKLSLAKTKNSESTIHVTCDLWAGDQSKQAEDPILVVQLHYFSESWQIRRPVVAFRHLNHTNLSMAVASELEGVLLSYGIFPRHIGYVLANQAKEILAGNNLFCDYKIVCSSNRREPDGDDILAFLSEKMSESEAPFSKLQIGTRNTCVVRSLQAVIRDALKNSRVVENLLSQIHNVVAFFRSSACWSELLLKEGNVSLCPSSTNCRWNSMMLSLRRMVHETTWSIVMTILAQARIEATDTSTAPPLIMVKREQVVDILGLLEPFEEALQVLQGNGVTMSFIIPSLIGLDKKLENRVTNYNHFCKALRMSLHAYFQSVIYQKEIILATVLDPRMKLQVLVMSRQPSRGYIISKTMKISELDVYLSEPMCESGSSLAYWKSASQFPQLQDIAKKLLAAPASSGGFDRLYPMATCIVRARRNRLPSHTTERLLLYKNSMKVRTASRVSGVPIPAKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KWC3</accession>
    <name>A0A3B5KWC3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Troponin T, slow skeletal muscle</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Slow skeletal muscle troponin T</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with TPM3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin T family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KWC3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002320"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002354.1">
        <property type="protein sequence ID" value="ENSXCOP00000002320.1"/>
        <property type="gene ID" value="ENSXCOG00000001859.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160609"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031014">
        <property type="term" value="F:troponin T binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006937">
        <property type="term" value="P:regulation of muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031444">
        <property type="term" value="P:slow-twitch skeletal muscle fiber contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000001">
        <property type="entry name" value="Troponin T, fast skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027707">
        <property type="entry name" value="TNNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521">
        <property type="entry name" value="TROPONIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11521:SF6">
        <property type="entry name" value="TROPONIN T, SLOW SKELETAL MUSCLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="37"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="37"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="96"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="196" mass="23649" checksum="230AA152FB8D7765" modified="2018-12-05" version="1">MEKDLLELQTLIDVHFEQRKKEEEELIGLKDRIERRRSERAEQQRVRAEKERDRQTRIAEERQRKEDEEAKKRADDEAKKKKVLSNMGAHFGGFLAKEEQRRGKRQTGREIKKKTLAERRKPLSIESLKEDGLRERAKEMWEWIYQLESEKFDLTEKMKKQKYEVNLHLNTAKTQNLTKYFRKKGHGKGKVGGRWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KWR7</accession>
    <name>A0A3B5KWR7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KWR7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002465"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002499.1">
        <property type="protein sequence ID" value="ENSXCOP00000002465.1"/>
        <property type="gene ID" value="ENSXCOG00000001959.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164472"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046332">
        <property type="term" value="F:SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042771">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF53">
        <property type="entry name" value="HOMEODOMAIN-INTERACTING PROTEIN KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="57"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="342" mass="39068" checksum="833B9AA42A6CFC27" modified="2018-12-05" version="1">MVASYVVATPALGQTDSTAALQLARLPESMGNKCCKKICKCPWTGQNVILKGSYCSYRVVRVIGRGVYGKVVKCKNLNTKETVAIKIIRKDLKHAGRSEILNMFSLRKFDSDRCSLIKMTDHFVHKEHQCLAFEILDTDLFEFMKGRHFKPLSVSAIRLIAQQMLVALQALKSIAMVHTDIKPDNIMLVNHKLIPFKVKLIDFGMAKHVSELRTGSWVQPNCYRAFEVLLGLPLSVSMDMWSLGCTLAFCYLGRLLFPSYSQYDNMKMFVELCGQPDNSLLNKGLFTSNFFNLVQETPEEIWELKDAKEYVMTSKDKFANARMDKDLHFWRHPLWSEEAEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXE2</accession>
    <name>A0A3B5KXE2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidylinositol N-acetylglucosaminyltransferase subunit P</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Part of the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PIGP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXE2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002296"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002330.1">
        <property type="protein sequence ID" value="ENSXCOP00000002296.1"/>
        <property type="gene ID" value="ENSXCOG00000001842.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013771"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017176">
        <property type="term" value="F:phosphatidylinositol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035973">
        <property type="entry name" value="Cyt_c_oxidase_su3-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052263">
        <property type="entry name" value="GPI_Anchor_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013717">
        <property type="entry name" value="PIG-P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016542">
        <property type="entry name" value="PIG-P_GPI19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08510">
        <property type="entry name" value="PIG-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008765">
        <property type="entry name" value="PIG-P_GPI19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81452">
        <property type="entry name" value="Cytochrome c oxidase subunit III-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="6 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="PIG-P" evidence="9">
        <location>
            <begin position="12"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR008765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="131" mass="14696" checksum="66E29C9FF8424C03" modified="2018-12-05" version="1">MVENSPSPLPERAIYGFVLFLGSQFGFFLYCLWAYVPEEWLHSVGLTYWPQKYWALAMPIYLLVALMVSLVVLFGINMNNTAPLDSSSSGTTRSHPNIAKLHLSVTDGGIPRLEDVSISEVNKMFYLSSET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L065</accession>
    <name>A0A3B5L065_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein Flattop</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Cilia- and flagella-associated protein 126</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme. Acts as a regulator of cilium basal body docking and positioning in mono- and multiciliated cells. Regulates basal body docking and cilia formation in multiciliated lung cells. Regulates kinocilium positioning and stereocilia bundle morphogenesis in the inner ear.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Flattop family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L065"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001204"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001220.1">
        <property type="protein sequence ID" value="ENSXCOP00000001204.1"/>
        <property type="gene ID" value="ENSXCOG00000000983.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177826"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038797">
        <property type="entry name" value="Fltp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34639">
        <property type="entry name" value="PROTEIN FLATTOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34639:SF1">
        <property type="entry name" value="PROTEIN FLATTOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22611">
        <property type="entry name" value="CFAP126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="192"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00033306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="213" mass="23740" checksum="CE227A79CCE024F6" modified="2018-12-05" version="1">MLNDKGQPLAHLPTCFHYEATHTKVSLRVPRGLRPSVPPARRPGFFCPSRYPTGRTLHDTWARKKTTKPKLAHVGHTSFIVNSRGHLLPGVKVCALNYSPVLFSFEGTWDLPTRIPAHHIDPTARSKDGLRRLKLWGICPPEEGKSESGRGSKNTDVGKQVRSTQTVFGNKQLPFSRWEINTNIKCEVLNPVSSGDTLDSSPESHINKILHVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2S0</accession>
    <name>A0A3B5L2S0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Glutaredoxin-2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Monomer; active form. Homodimer; inactive form. The homodimer is probably linked by 1 2Fe-2S cluster.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2S0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006613"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006694.1">
        <property type="protein sequence ID" value="ENSXCOP00000006613.1"/>
        <property type="gene ID" value="ENSXCOG00000005118.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164211"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015038">
        <property type="term" value="F:glutathione disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0008347">
        <property type="term" value="P:glial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003147">
        <property type="term" value="P:neural crest cell migration involved in heart formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001944">
        <property type="term" value="P:vasculature development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000093">
        <property type="entry name" value="Glutaredoxin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011899">
        <property type="entry name" value="Glutaredoxin_euk/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02180">
        <property type="entry name" value="GRX_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46679">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46679:SF1">
        <property type="entry name" value="GLUTAREDOXIN-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00195">
        <property type="entry name" value="GLUTAREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13" id="KW-0318">Glutathionylation</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="18">
        <location>
            <begin position="68"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00039819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="170" mass="18808" checksum="1F78B6977B2A9937" modified="2018-12-05" version="1">YYHISKVIKCALPCVLVFIVLFSFDCAKWTYVFYSFFRMGNLISSSTGSLSSTACVQLVQEMVSQNCVVIFSKTTCPYCKMAKNVFNEIGANYRVIELDQHNDGKRLQEALAQLTGVRTVPRVFVNGNCIGGGSDTKQLHQQGKLLPLIERCSPCCITEGSGSGHFEPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L320</accession>
    <name>A0A3B5L320_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L320"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002179"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002210.1">
        <property type="protein sequence ID" value="ENSXCOP00000002179.1"/>
        <property type="gene ID" value="ENSXCOG00000001738.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063836"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052297">
        <property type="entry name" value="RING-CH-type_E3_ubiq-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14471:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14471">
        <property type="entry name" value="MARCH7/10 E3 UBIQUITIN PROTEIN LIGASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-CH-type" evidence="10">
        <location>
            <begin position="545"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="21"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="170"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="508"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="661"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="170"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="206"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="246"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="451"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="512"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="690" mass="75300" checksum="6638A302FDB01274" modified="2018-12-05" version="1">MDSRSRRLPFCLSSSRSSYTFTPAVSSSLGSSRRYSRDPVLNNDRFQRASSPYKADTDKQSSRLLSSSRDYSSSDTRSPSWKLPSLTSSGRSYDRPWVESALSSRSKAVLNDAEGRSGICSGVLTASDDAESKRAKLSYSNRLYSRTTNTSLTGTNNSLDSSWGSYRLLSRPTPSSTKTLLSSREQETKTEPGLSSLGERRARNSGLASSLYQTDRVTSTYAQGARPKETTYSSSSSAVRESSLSRHASSFSQQPTSSQLHDLSSRSTARFTNAPPSLRSTQEQARSTLSSGVFASHSSSSSSSSLSSHTPWQSTPLSKPELALPRPSAEGGEPEGRSSTRRLLSRLFSRCSSQDSSSCSSSVRSLDDDSPSTSGESVDSDEGAGPSSADPDNGGAATALDSLRSHRTDLSTIQESNPGHGGLGRSGTASWREPSVSSSSSSGGGGRASQDDDEEEDEDDDEEEEEGAFALDAFAESYADGIEDEALPELEVVFSPRRQLGVLENLSASLGRGERQPESDQKEKTLSSKDQEKLRKIKENKWVDSDEEEGDLCRICQMREESSSNPLIQPCHCTGSLQFVHQECIKRWLRSKISSGNLQIQELKLHHGFCRSEYDEFISSGLYLVVLLHFCEQRFSDVLGAVDAAGLFNLVRILHEHMDSLETSTGESDEEEQDSRPSVDFSDLDYDEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5C2</accession>
    <name>A0A3B5L5C2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutaredoxin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5C2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006080.1">
        <property type="protein sequence ID" value="ENSXCOP00000006012.1"/>
        <property type="gene ID" value="ENSXCOG00000004664.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014940"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000628">
        <property type="entry name" value="Glutaredoxin-like protein C5orf63 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008554">
        <property type="entry name" value="Glutaredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052565">
        <property type="entry name" value="Glutaredoxin-like_YDR286C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33558">
        <property type="entry name" value="GLUTAREDOXIN-LIKE PROTEIN C5ORF63 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33558:SF1">
        <property type="entry name" value="GLUTAREDOXIN-LIKE PROTEIN C5ORF63 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05768">
        <property type="entry name" value="Glrx-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="105" mass="12226" checksum="CA8B7E70E420DFDC" modified="2018-12-05" version="1">MSVHRTLCGRPALQLCRMFSSTSLPTLTLFTKDPCPLCDEAKELLQPFKHRFILQQVDISLPENRQWWDKYKWDIPVFHLNGQYLMKHGVDVALLDKLALWATNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5G0</accession>
    <name>A0A3B5L5G0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Arginase</fullName>
            <ecNumber evidence="3 13">3.5.3.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>L-arginine + H2O = urea + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:20569"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="EC" id="3.5.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10 13">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the arginase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.1" evidence="3 13"/>
    <dbReference type="RefSeq" id="XP_027856624.1">
        <property type="nucleotide sequence ID" value="XM_028000823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5G0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005640"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005706.1">
        <property type="protein sequence ID" value="ENSXCOP00000005640.1"/>
        <property type="gene ID" value="ENSXCOG00000004392.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114134305"/>
    <dbReference type="KEGG" id="xco:114134305"/>
    <dbReference type="CTD" id="384"/>
    <dbReference type="GeneTree" id="ENSGT00950000183195"/>
    <dbReference type="UniPathway" id="UPA00158">
        <property type="reaction ID" value="UER00270"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004053">
        <property type="term" value="F:arginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010121">
        <property type="term" value="P:L-arginine catabolic process to proline via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09989">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.10:FF:000005">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014033">
        <property type="entry name" value="Arginase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01229">
        <property type="entry name" value="rocF_arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43782">
        <property type="entry name" value="ARGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43782:SF4">
        <property type="entry name" value="ARGINASE-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0056">Arginine metabolism</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 10 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0835">Urea cycle</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036979-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="346" mass="37310" checksum="4A5D69A7D9EE0433" modified="2018-12-05" version="1">MALRGPLSHLLRTQLRQTCQQSRAQSVAVLGAPFSKGQKRGGVEHGPKAIRDAGLIERLSSLDYSVHDFGDLSFHLPETDEPHMNVKSPRSVGGANQKLSRAVSRAIGAGHTLVMLGGDHSLAIGSVSGHAQQCPDLCVIWVDAHADINTPMTSPSGNLHGQPVAFMLKELQDKMPDIPGFSWTKPFLSSRDLVYVGLRDVDPGEHHILKNLGIQYFTMRDIDRLGIQRVMEVALDHLLARKQRPIHLSFDIDAFDPSLAPATGTPVNGGLTYREGIYVTEEIHNTGLLSAIDLVEVNPVLGANQEAVEATASLAVDVIASSLGQTREGAHASIPAEKSDTEQLCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L663</accession>
    <name>A0A3B5L663_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Desmosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Cell membrane</location>
            <topology evidence="13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L663"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005895"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005961.1">
        <property type="protein sequence ID" value="ENSXCOP00000005895.1"/>
        <property type="gene ID" value="ENSXCOG00000004573.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234624"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030057">
        <property type="term" value="C:desmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045216">
        <property type="term" value="P:cell-cell junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055113">
        <property type="term" value="P:epiboly involved in gastrulation with mouth forming second"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000011">
        <property type="entry name" value="Cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000031">
        <property type="entry name" value="Cadherin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000346">
        <property type="entry name" value="Desmocollin b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000068">
        <property type="entry name" value="Desmoglein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000074">
        <property type="entry name" value="Desmoglein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050971">
        <property type="entry name" value="Cadherin-domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009122">
        <property type="entry name" value="Desmosomal_cadherin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24025">
        <property type="entry name" value="DESMOGLEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24025:SF32">
        <property type="entry name" value="DESMOGLEIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01818">
        <property type="entry name" value="DESMOCADHERN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 12 13" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 13" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="45"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="134"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="239"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="390"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="390"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1070" mass="116208" checksum="494799BB171BD185" modified="2018-12-05" version="1">MISADAKEKGLKTLKRAKREWILPPAKLKENHNYTHLEFIAKIRSDRDKQRRVEYSLTGAGADKPPFNLFVVDHNTGFIRITDILDREKYPSYNLTGIAKFKDGISAEENIPLTVTVLDENDNPPYFVLHKGNVTEASREGTFVMQIEGKDDDEAGTINSEIVYTIISQEPAGKGHMFTIDKKTGKLFVKESTLDREAYDFYKLVVKGTDMGGAPSGKTGTGTVEIKVTDINDNIPTLEKSEYAGSVDENVADVVVMRIKALDKDLLHTNNWLTVFTIAQGNEDNLFSIETDKETNEGILKLIKPVDFEEVQNLELGLIIENVAPFVEGGPIKMDVDVQVGEGGPLAAGAAAGAGAGVGVGVGAGVDLGVDLGYPIKIAVNNVPEGPAFRPDTKKVPVSEDPNEAPADGVITSFPAVDPDTGKVADDVSYAKAYDPDNWFTVDEETAEIKLNKQPDRESPFLVNGTYIAKILAISKDMPAKTATGTIAIQVTDSNDHCPTLTTTYTSLCSDKKTVYVTAFDEDVSPNAAPFTFQIIPEGTRGNWEIEVINDTTAALHSAKALWPGRYELQVEVKDAQGLSCKEERVFTVDVCTCVGKEDCRSKASRGEARSSDFSRRAIGPIVTALFLLMLIPLLLVFCQCGGANTIFSDQFSDLPFDTKEHLINYHTECIGEDKVYLLNKYTYLRTLSVILVLFLFPKQKSTITVPSEDILLEYDFEGKGSSAGSVGSCSILEPDNDLNFLTDLGPKFRTLAEICSPPTLTSKAMVIQKASGSVKTTTDITEPVFKPKFNYEIQAKHVDDKAEKVVSSAKVVQSHVSTTTKESTTKQLTTTTLPHSTSTNISHSAKLSYPAQTVVLQQPPVYYTASPVLQPMHYIVQPNVQNTFVLEDGTHANTFPGLYVINDPKSMSGLVYGRTQSSLSGLVIQDTEGPKSPISPVSPTGSLRTTVLLPSPTFSQGCVEGSTLYGQNLGGNYVLLKDEVQEWGPGSPQGILPGDAILVKKAAPPQGVLGLAAQENVCISSFCYFLVKKANGGGGVIVTGGISFGSFSVLKYLTKQQRMWIWLNKVKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6D6</accession>
    <name>A0A3B5L6D6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SEFIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6D6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006452.1">
        <property type="protein sequence ID" value="ENSXCOP00000006372.1"/>
        <property type="gene ID" value="ENSXCOG00000004926.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168503"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030368">
        <property type="term" value="F:interleukin-17 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039465">
        <property type="entry name" value="IL-17_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013568">
        <property type="entry name" value="SEFIR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15583">
        <property type="entry name" value="INTERLEUKIN-17 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15583:SF12">
        <property type="entry name" value="INTERLEUKIN-17 RECEPTOR C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08357">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017227189" description="SEFIR domain-containing protein" evidence="10">
        <location>
            <begin position="22"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="SEFIR" evidence="11">
        <location>
            <begin position="386"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="597" mass="66480" checksum="E8E36115BA1EC34F" modified="2018-12-05" version="1">MFLFKSSLWCIVFSLCSSACGLEFIKYDRDAVICQQAFSDCTIRNGLYEQNDNHVQFEASFKLCCKAERNCTLCLVLETEISNIPMDKDVENDNHSGTDEEDDNEDTTLERSSVMACYHDPASTMPGCKKVEFTVNYTTLAHQNSSKVLNRSKHVQQYDTRFSLSTVCSQESFQQPSVLICFKSPKCCVNNMNSCKTESANSTFGSLAQTTIPIHSVAPCMCIQVWDKDDEGAVRSQLCHFDKIGSPPPLQHLMSKNVSVNVRLSKMGDRNTVLLWNLTAPCRLDGEVSLCLKPSSSENLAGVSNWKQNSMGLWVRTALSTSTVFRWRWTLLIVGVLLVGCLTALMIHFLRDYVKSNTSLNLVFFFSLVGRKGHVVLLSPPDLDDTVSESVCLLGSQLCSQGFSVSVDQWCRKDQCTMGPLPWLYSQLQKLDNMGGRVLLVLTQKALEKTEEWTLLNNNGEGRDQQQMKSPYSDLFTASLFIIQAHKRLGRVAERFVLVKIDSHQTQSHSSDGRLPELLQGLPLFLLPSQSQSLTAELIVEEAETTRISLSPNNRCYTDRFISCSDGREVATENAAASCVAVQRAGSSSGQQLPACL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L795</accession>
    <name>A0A3B5L795_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">G protein-coupled receptor, class C, group 5, member C</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L795"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006310"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006386.1">
        <property type="protein sequence ID" value="ENSXCOP00000006310.1"/>
        <property type="gene ID" value="ENSXCOG00000004881.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182961"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15277">
        <property type="entry name" value="7tmC_RAIG3_GPRC5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051753">
        <property type="entry name" value="RA-inducible_GPCR3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14511">
        <property type="entry name" value="G PROTEIN COUPLED RECEPTOR, CLASS C, GROUP 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14511:SF15">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY C GROUP 5 MEMBER C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="8">
        <location>
            <begin position="79"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="394"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="412"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="469" mass="51359" checksum="4CD9518F6674C6A0" modified="2018-12-05" version="1">ILFIVYLMPCFWFSQYSFLLLNPGCGLDLDSLYYNLCSLSAVWGIVVEAIAAAGVIFSFVLFVSLLASMPFIKSPNHKNSLALQASFLFCTAGLFCLTFAFIVGKNFSTCASRRFLFGVLFGGCFACLLMQCIRLNILARRNAGPRGWVLWLGALGMWLVEVVINTEWLIITVVRHPLLPVNATTEPIRATATPCNIANQDFVMALIYVMVLILATVMAGLPIMAGKYAEWKKEGGLILVISLISVGIWVAWIVMYVYGNEQTGGPTWDDPTLAIALVANGWVFIIIYTIPQICCFNSDDEIEEDYGEDAYTNRGVGYETILKEHNSQKMFMENKAFSMDEPGQGNKPVSPYSNYTGQMRATVYQPTELALITKTVGNREQSFDMIIPRASMASAANSGSSTPSTHTEHGNTSRLQTSVGQPSAPPPTLLLFFTVLIISCCPPDAFLFFVSEHVALSAPAVSMATSSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8T8</accession>
    <name>A0A3B5L8T8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gypsy retrotransposon integrase-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8T8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008713"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008819.1">
        <property type="protein sequence ID" value="ENSXCOP00000008713.1"/>
        <property type="gene ID" value="ENSXCOG00000006655.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000170215"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000032">
        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="101"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="358" mass="41366" checksum="87D9854CCCB1C3F9" modified="2018-12-05" version="1">YIYYATVVLCNPLVIMLKIKPKKSQLKYAEKFTIHAGDLFYGALPERRAIRSKEEAWNLFKEFHASPMGGHTGIVKTRIAMCSRFYWPGMTADIEKWVCQRTSVHKGVWELIGIDLTGPMPLTSCGNQYILTATDYFSKWVEAFPLKTKTAEEVCQKLCSIFYWHGCPQCILTDQGREFMNSRLCDLLSIQRSITAAYHPQTNGLDEKTNDNIKRALRKLVNDKQDDWDMFLEATLFSLRSKTHTTTKYSLFFLMHGREARFPSEVPVDMPVRFCYITVYICTIFVFLYYYICTVFAWKSECIKVLSLYIMLKFQQSRNPTADQDRRSGPWQGHPVLFPVVSLSSSISPCQVLSVVFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L961</accession>
    <name>A0A3B5L961_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Growth factor independent 1B transcription repressor</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L961"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007005"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007093.1">
        <property type="protein sequence ID" value="ENSXCOP00000007005.1"/>
        <property type="gene ID" value="ENSXCOG00000005412.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160010"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060216">
        <property type="term" value="P:definitive hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030218">
        <property type="term" value="P:erythrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="204"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="18"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="252" mass="28406" checksum="239FD0262A8509F6" modified="2018-12-05" version="1">MPRSFLVKNKRCASYNIHRSYEEEQPLQSTDSSEEPLSEGPSADRCSPPEDSEPQCPLPAHPEPASPPLAPSQPYYLTEPHMAEFPPYYKPSYAWESVSSSYKLRQLSFSPTVLQHASSLYGGHISHSPPPQQPLDCSTHYSPTSNTYHCITCDKVGSFSHHLKSTFVCNFPKLFLLFFVVQVFSTSHGLEVHVRRSHSGMRPFGCSICRKTFGHAVSLEQHMNKKHENPHFTKTRGGFLNMARCHGEKRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAL3</accession>
    <name>A0A3B5LAL3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Doublecortin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027887681.1">
        <property type="nucleotide sequence ID" value="XM_028031880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAL3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009238"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009349.1">
        <property type="protein sequence ID" value="ENSXCOP00000009238.1"/>
        <property type="gene ID" value="ENSXCOG00000007045.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114153367"/>
    <dbReference type="KEGG" id="xco:114153367"/>
    <dbReference type="GeneTree" id="ENSGT00940000154895"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042461">
        <property type="term" value="P:photoreceptor cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060041">
        <property type="term" value="P:retina development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16112">
        <property type="entry name" value="DCX1_DCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17069">
        <property type="entry name" value="DCX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.230:FF:000003">
        <property type="entry name" value="Neuronal migration protein doublecortin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.230:FF:000001">
        <property type="entry name" value="serine/threonine-protein kinase DCLK1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.230">
        <property type="entry name" value="Doublecortin domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003533">
        <property type="entry name" value="Doublecortin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036572">
        <property type="entry name" value="Doublecortin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23005:SF4">
        <property type="entry name" value="OXYGEN-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23005">
        <property type="entry name" value="RETINITIS PIGMENTOSA 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03607">
        <property type="entry name" value="DCX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00537">
        <property type="entry name" value="DCX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89837">
        <property type="entry name" value="Doublecortin (DC)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50309">
        <property type="entry name" value="DC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Doublecortin" evidence="11">
        <location>
            <begin position="53"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Doublecortin" evidence="11">
        <location>
            <begin position="180"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="277"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="297"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="310"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="340"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="350" mass="39089" checksum="112F8467B7AC47C0" modified="2018-12-05" version="1">MELDFGHFDERDKTSRNPRSGRMNGLPSPTHSAHCSFYRTRTLQALTNEKKAKKVRFYRNGDRYFKGIVYAVANDRFRTFDSLLADLTRSLSDHINLPQGVRFIFTIDGSRKIASLDELEEGESYVCASENFYKKVDYTKNVNPNWSVNVKASASQKNMQSLAAKAAGDPREGKDFVRPKLVTVMRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLESGVVKRIYTLDGKQVTCLQDFFGDDDVFIACGPEKFRYAQDDFSLDENECRLMKGAKAQHGSVKSPGPIKRCKSPADSTNGTGSSSQISTPISKHSPTSTPTSPGLNNKQKDLYLPLSMEDEDSLGESM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBJ9</accession>
    <name>A0A3B5LBJ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4939 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBJ9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005249"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005310.1">
        <property type="protein sequence ID" value="ENSXCOP00000005249.1"/>
        <property type="gene ID" value="ENSXCOG00000004109.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000173342"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="14476" checksum="F95DDD2138936110" modified="2018-12-05" version="1">MLSHALVWPSQSSLKIDICRCSSSNLTPDPAPTPASSADGTIRSMFSECRPPAPERFSGDASNFSHDGAKILFVLSHLSGKALDWAESRFSPSSDYGCSFEDFLKEFKQVFNQVSDRASDSRDLETMEKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDB1</accession>
    <name>A0A3B5LDB1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Bloodthirsty-related gene family, member 12</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDB1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008535"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008641.1">
        <property type="protein sequence ID" value="ENSXCOP00000008535.1"/>
        <property type="gene ID" value="ENSXCOG00000006533.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167415"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19802">
        <property type="entry name" value="Bbox1_TRIM8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13733">
        <property type="entry name" value="SPRY_PRY_C-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF49">
        <property type="entry name" value="BLOODTHIRSTY-RELATED GENE FAMILY, MEMBER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="9"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="10">
        <location>
            <begin position="160"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="11">
        <location>
            <begin position="364"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="211"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="561" mass="63274" checksum="2B7C2296A18D7373" modified="2018-12-05" version="1">LQNKDQFTCSICLEIFVEPVSTPCGHSFCKACLQGYWNHSKKFLCPMCKKSYSRKPEMSVNRVLAEISSQFQGLVVSGSPSRGGSITISSDSGHSGPPAQDTGEFAQPGDIPCDACIGRKLKALKSCVNCPGSYCEAHLRHHKKVKTLTSHFLIEPTFHLEEKICKKHERLLEVFCRTDHTCICKACAEVTHKSHDIISVDHEFKKKMTNLGKKRSELKNLIKERAKKLEEIKQSIKVIKASSQKELEDSWQVYATLQKLVEQSQAEMVELIATKQREAERHAQELARSLENELSQLRRRSNELEAHAHTKDKVVFLQNLATLSHSPEPTDWSGVSINTDIYLGTLRSSVSGLIEKFQDELKRLYGKGERSQSYPALSFPFLGEVILDTSTAQKNLVVSQEGQQVKYEEHKVSHSEGPKRFNPALFVLAREGIHSGRHYWEVEVGRKTAWTLGVARATARRKGEIKLNPEGGYWCLWLKNGEVKALAETRVPLALMSLPSKVGLFLDYEAGQLSFYDVTARQHLYTFTEKFNDSVYPIFSPCLSQDGKNNSPLIITQVKYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LE46</accession>
    <name>A0A3B5LE46_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">XK-related protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Phospholipid scramblase that promotes phosphatidylserine exposure on apoptotic cell surface. Phosphatidylserine is a specific marker only present at the surface of apoptotic cells and acts as a specific signal for engulfment.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the XK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LE46"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009223.1">
        <property type="protein sequence ID" value="ENSXCOP00000009112.1"/>
        <property type="gene ID" value="ENSXCOG00000006949.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282533"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902742">
        <property type="term" value="P:apoptotic process involved in development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043652">
        <property type="term" value="P:engulfment of apoptotic cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070782">
        <property type="term" value="P:phosphatidylserine exposure on apoptotic cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018629">
        <property type="entry name" value="XK-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050895">
        <property type="entry name" value="XK-related_scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16024">
        <property type="entry name" value="XK-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16024:SF16">
        <property type="entry name" value="XK-RELATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09815">
        <property type="entry name" value="XK-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="220"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU910716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="335" mass="38755" checksum="18BB279977918E5B" modified="2025-10-08" version="2">MQQEKHKRQGQKFRWQLWMTAAASLVSLAWALASYQKALRESRDDKKPIGYLAVIIQFCWHFFTIAARVVTFALFASVFQLYFGIFIVLHWCIMTFWIVHCETDFCISKWEEIVFDMVVGIIYIFSWFNVKEGCTRYRLSIYYLVILVENATLSALWYLYRTPKTTDAFAVPALCVIFSSFLTGLVFMLMYYAFFHPNGARFGRSASCQGLDMNPTAQFSTLPSDGGTNSLRSNRGATGTLERDTGKYSERDSCIPVFQVRHPVPSTPSSRAPRLDETVIKLDLCRNQYPAWERHVLDRGIRKVILAMDCSMTPPRLMYKDDAMVHERLEYETTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEL8</accession>
    <name>A0A3B5LEL8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">MCF.2 cell line derived transforming sequence b</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LEL8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009015"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009124.1">
        <property type="protein sequence ID" value="ENSXCOP00000009015.1"/>
        <property type="gene ID" value="ENSXCOG00000006874.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156974"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016358">
        <property type="term" value="P:dendrite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000078">
        <property type="entry name" value="Guanine nucleotide exchange factor DBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051336">
        <property type="entry name" value="RhoGEF_Guanine_NuclExch_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056466">
        <property type="entry name" value="Spectrin_DBS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826:SF207">
        <property type="entry name" value="PROTO-ONCOGENE DBL-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826">
        <property type="entry name" value="RHO GUANINE EXCHANGE FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23289">
        <property type="entry name" value="Spectrin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="4">
        <location>
            <begin position="472"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="664"/>
            <end position="780"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="787" mass="90892" checksum="F77039A0985EE272" modified="2018-12-05" version="1">TSFPGNLHLVLVLRPTSLLSSTPSPSSSTDLGFRFSQDDFLLKMPVVMLRSVGDLLRYIDENHLASDFTAKVEYCQSDWVLLRSSIETFAVTVKEIAQLLQGFGSELSETELPDEANAIEFLLHSHSHRYRQMKQDDIRGVLKEGRLLLSNLETVKAAKREAEEEREIQADMETVQLLAQLRDMEEAFDGFFEKHHLKLQQYLQLLHYESSFQQMDEVLERISAQEKEIACVGTTVVQTEQLLKDLDSLDKRAQQEEMNRAQVVILHGHQLAANHHYALALIVQRCNELRHHCDVISTAVRTKRASLTRTRDLLRRLEEALRWCDEGAYLLASQMVDKFQTKEGAQEALQYLSSHQERAPSVVKNTQDTLSLEFEAVLTPQLQITMVKEKLSSVQCMIRNREQCLKKLADVQVRPIQLVAPRPEPDQTVLRCKSLLFSPKHIEVMHDYQGNRSCLYGPNPETDSEAEDNPEQVTHIMKELIATERIYVDELLSVLLQGYRAEMEDPSTSYLLPSALRCQKDVLFGNMPEIYQFHSIFLHDLQACLETPERVGACFLQRKDKFQVYERYCQNKPCSELLWRQCSDSPFFQECQKKLDHKLGLDSYLLKPIQRLTKYQLLLKELLKHCVEEHYRCELQEALDSMLELLKSVNDSMHQIAITGYPGDISQLGRIILQGNFSVWISHKRAAVRMKELARFKPMQRHLFLYDHALLFCKRRDEDSQDSFYSFKSCMSTVAITETVKGDVKKFEICYSGREVVYLVQAPTVEVKVAWLNEIQKILANPQKLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LET2</accession>
    <name>A0A3B5LET2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RNA 3'-terminal phosphate cyclase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RCL1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">As part of the small subunit (SSU) processome, it plays a role in 40S-ribosomal-subunit biogenesis in the early pre-rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation of the 18S RNA. Activates BMS1 by promoting GDP/GTP exchange. Does not have cyclase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3. Interacts with BMS1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNA 3'-terminal cyclase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027881514.1">
        <property type="nucleotide sequence ID" value="XM_028025713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LET2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006369"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006448.1">
        <property type="protein sequence ID" value="ENSXCOP00000006369.1"/>
        <property type="gene ID" value="ENSXCOG00000004922.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114149687"/>
    <dbReference type="GeneTree" id="ENSGT00530000063404"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00875">
        <property type="entry name" value="RNA_Cyclase_Class_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.20:FF:000001">
        <property type="entry name" value="RNA terminal phosphate cyclase-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.65.10.20:FF:000001">
        <property type="entry name" value="RNA terminal phosphate cyclase-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016443">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03400">
        <property type="entry name" value="18S_RNA_Rcl1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF1">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005378">
        <property type="entry name" value="RNA3'_term_phos_cycl_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="8">
        <location>
            <begin position="7"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase insert" evidence="9">
        <location>
            <begin position="181"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00056741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00065529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="372" mass="40912" checksum="210A9E755A178F4E" modified="2018-12-05" version="1">MASHGLTYDGCNFFRQRLVLSTLSGKHVKIKNIRSRDDEPGLRDFEASFIRLLDKVTNGSRIEINQTGTVLFYQPGLLYGGPVEHDCNPQRSVGYYLEALLMLAPFMKTPLKATLKGVTNDPTDPTVDLLKSTALPLMKKFGIDGEAFEIKVVKRGMAPGGGGEVVFTCPVRKTIKPVQLTEPGKIKRIRGVAYSVRVSPQMGNRIVESARSVLNQFLPDIYIYTDHMKGTNSGKSPGFGLTLVAETLNGCFLSAEMSSTPQGQGDPVLPEDLGRNCAKLLLEEVYRGGCVDSSNQSLALLLMTLGQQDVSKVLLGPLSPYTIEFLRHIRDFFQIMFKIEVQTPLDDERKGGDKVLMTCVGVGYSNINKTVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LF89</accession>
    <name>A0A3B5LF89_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription factor 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LF89"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009240"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009351.1">
        <property type="protein sequence ID" value="ENSXCOP00000009240.1"/>
        <property type="gene ID" value="ENSXCOG00000007043.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159129"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18945">
        <property type="entry name" value="bHLH_E-protein_TCF4_E2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000001">
        <property type="entry name" value="Putative transcription factor 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051098">
        <property type="entry name" value="NeuroDiff_E-box_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11793">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11793:SF10">
        <property type="entry name" value="TRANSCRIPTION FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="9">
        <location>
            <begin position="305"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="185"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="235"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="296"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="414" mass="43927" checksum="D0A9ABD7174C065B" modified="2018-12-05" version="1">MHGDPWSSSSSSMSQQGYHSSMLGGGNSAHGPAQSSSYCGIHPHDRLSYPSHSSSADLSSSLPPMSSFHRAGGGGGGGANHYSTASCTASTNGTDSVMDGASSPNYEAPLHSLQSRIEDRLERLDDAIHVLRSHAVGPSTGMASAHGDMHNLIGAAHTHNGAMGALSSGYGTGLLSANRHSLMVGSHREDGGSVGSLRGGHSMGGQVSVPQLPVQSATSPDLSQPDPYRGKARPLSGGLQGQSTSSVSSEIKSEDEGDENLLQDNKSLDKKDDLDKELKAIERSRSSNNDDEDLSPEQKMERERERRMANNARERLRVRDINEAFKELGRMVQLHLKSDKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVTVTSDGTPLSLAAAHHAAAAAMGDGPNPMGQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFE7</accession>
    <name>A0A3B5LFE7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Phosphoinositide-3-kinase, regulatory subunit 2 (beta)</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3K p85 subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFE7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009300"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009411.1">
        <property type="protein sequence ID" value="ENSXCOP00000009300.1"/>
        <property type="gene ID" value="ENSXCOG00000007077.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157050"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046935">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="iSH2_PIK3R2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH2_cSH2_p85_like"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="phosphatidylinositol 3-kinase regulatory subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000006">
        <property type="entry name" value="Phosphatidylinositol 3-kinase regulatory subunit alpha"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Putative phosphatidylinositol 3-kinase regulatory subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PI3K_P85_iSH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035020">
        <property type="entry name" value="PI3kinase_P85_cSH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035022">
        <property type="entry name" value="PI3kinase_P85_nSH2"/>
    </dbReference>
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        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
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        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16454">
        <property type="entry name" value="PI3K_P85_iSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00678">
        <property type="entry name" value="PI3KINASEP85"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2 8" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="12">
        <location>
            <begin position="3"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="13">
        <location>
            <begin position="41"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="11">
        <location>
            <begin position="273"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="11">
        <location>
            <begin position="560"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="84"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="380"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="466"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="662" mass="76368" checksum="02387FFAB312D354" modified="2018-12-05" version="1">MATDGFQYRVLYTFTKDQEEDLDLQPGDLLTVSKASLMSVQSYEEGFEEHPERLGWILGYNERTMQRGDFPGTFVEFVGPIKMAHPSSQPRSQRPLPAAPIPEPSQLSQQFTPPETAPPDLISLIQAIEDVGFLSESVLCCLRDLPSPVIPACIYPQLQTAEVSLVLENTTVVPLHHRLTLQYLLTHLTKVLQAQASNGLDTHTLGRIFGPLLIRTSPSNQYSWEEDFPVLVVERLLVERTAQQDFDPPKPTKSRIAPSDMTDTSSLLNDAEWYWGEISEEVNEKLRDTPDGTFLVRDASSKLEGEYTLTLKGGNNKLIKIYHQEGRYGFSEPLTFQSVVELINHYRHESLAQYNAKLDTKLLYPISKFQQAQLVKENSIEEVGEQLKVYHEQYQEKSREYDSLYDEYTRTSQQELQMKRTAIEAFNETIKIFEEQFETQEKYSRESLERFQREGNEKEIQIQSNSERLKSRVKEIHDSKRKLEQDLKQQVSDNREMDKKMNSLKPDLMQLRKIRDQYLWLTQKGTRQKKINEWLGIKIDADSYSLEEDDSSPHHDDCTWYVGDIKRSQAEEMLRDKCDGTFLIRESQSQKGSYACSVVDGDTKHCVIYKTATGYGFAEPYNLYSSLQELVLHYKNVSLAQHNDHLNVNLAYPVLARSQNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFM2</accession>
    <name>A0A3B5LFM2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sodium/glucose cotransporter 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">High affinity sodium-glucose cotransporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Solute carrier family 5 member 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>D-galactose(out) + 2 Na(+)(out) = D-galactose(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70499"/>
            <dbReference type="ChEBI" id="CHEBI:4139"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:70500"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>D-glucose(out) + 2 Na(+)(out) = D-glucose(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70495"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:70496"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFM2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006669"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006750.1">
        <property type="protein sequence ID" value="ENSXCOP00000006669.1"/>
        <property type="gene ID" value="ENSXCOG00000005159.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155844"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005412">
        <property type="term" value="F:D-glucose:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1730.10:FF:000051">
        <property type="entry name" value="Low affinity sodium-glucose cotransporter-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018212">
        <property type="entry name" value="Na/solute_symporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819:SF151">
        <property type="entry name" value="SODIUM_GLUCOSE COTRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00456">
        <property type="entry name" value="NA_SOLUT_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00457">
        <property type="entry name" value="NA_SOLUT_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="14" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="83"/>
            <end position="106"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
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            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
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            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
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            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="455"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="497"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="607"/>
            <end position="630"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00040046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="631" mass="69760" checksum="9F338C145222D04E" modified="2018-12-05" version="1">MSWDYFGFSRIIRNTENQSPASALNNAADISVIVAYFVVVLAVGVWAMVRTNRSTVGGFFLAGRSMVWWPIGASLFASNIGSVHFVGIAGTAAAAGIAICGFEWNVSNPSLSLTQAQKFTLRGDLKVYRFLFVKADMFSGAIFIQLALGLNIYVAVIALLAITALYTVTGGLAAVMYTDTLQTIIMLIGSFILMGFAFNEVGGYENMMDRYMTSVPLITGNISAECYQPREDAFHLFRDPINGDLPWPALIFGLTVQTIWYWCTDQVIVQRCLSAKNLSHVKAGCILCGYLKLLPMFLMVFPGMISRILYPDVVACVDPEECQKYCGSSVACTNIAYPKLVMDLMPNGLRGLMLAVMLASLMSSLTSIFNSASTLFTMDIYTKVRSRAKERELMIAGRVFIVFLIGVSIAWIPVVQSAQSGQLFDYIQSIISYLAPPISAVFLLAIFCKRVNEAGAFYGLIVGLIVGLTRMITEFAYGTGNCVSPSDCPAIICGVHYLYFGLILFTITCIVIVGVSLITKPIDDKHLHRLCWSLRDSTEERVDLEQDDWVNDEEDEKKMEEPEEEPGFCKKAVMCFCGLEKTKAPKMTEEEKAEMKRKLTDTTEEPLWRNVVNANAIILLAVAVFFHGYFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LG27</accession>
    <name>A0A3B5LG27_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Upregulator of cell proliferation</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000008631"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008737.1">
        <property type="protein sequence ID" value="ENSXCOP00000008631.1"/>
        <property type="gene ID" value="ENSXCOG00000006600.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154390"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030383">
        <property type="entry name" value="G_VLIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057365">
        <property type="entry name" value="URGCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052986">
        <property type="entry name" value="VLIG_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14819">
        <property type="entry name" value="GTP-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14819:SF9">
        <property type="entry name" value="UP-REGULATOR OF CELL PROLIFERATION-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25496">
        <property type="entry name" value="URGCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25683">
        <property type="entry name" value="URGCP_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Up-regulator of cell proliferation-like" evidence="1">
        <location>
            <begin position="83"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="VLIG-type G" evidence="2">
        <location>
            <begin position="406"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="548" mass="62418" checksum="45248E4F92FF7DD4" modified="2018-12-05" version="1">IDLIEKISLSKILEIDEKTITDQPVKCKSEIPWYFLKKQMMVNVTARNVKLTSGCDLVCKDAVGTAKLDLRNLLNIPDTDDTLNPLDLITALFLCSDGFVQQELALKLSMCQFSVPLLLPNCDTQQCTLMLWAMRDIVKRYRPPALSESKGFIEERIVNSELPMISFVRLGECSLSKSEILNKLLSNSQQYHDTFVHRDMESGDVQRRISNGLTEMTWYLPCGNKNMDIFSEPVAIANLRGDIASFETQFSFLCQTSAAVFVFFESEHSGLEFLTQQNCKAQIFLVGNVRSKTFSLDALEEVATKLCLTNNNIILKTKSMNDADFVKCLRKTVNDVVHSQHIKISVENMVSVAHELGVLVEEDAPDCQAGKINADAITAEIENIPQYKEEHLPLQGQIWRELTCLEKEEFRLQKVGSQNIEHYRSNLHEQKTELRKRQNSYAMSPAMRCFINAISTKGMERCYFLKWMQMNLDNLSRIKLSGLRELYKEKSKDSGNKEEIKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSFRHVCSLDTICL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LG41</accession>
    <name>A0A3B5LG41_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zinc finger matrin-type protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Acts as a bona fide target gene of p53/TP53. May play a role in the TP53-dependent growth regulatory pathway. May contribute to TP53-mediated apoptosis by regulation of TP53 expression and translocation to the nucleus and nucleolus.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with dsRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LG41"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011113"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011240.1">
        <property type="protein sequence ID" value="ENSXCOP00000011113.1"/>
        <property type="gene ID" value="ENSXCOG00000008397.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111202"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000285">
        <property type="entry name" value="Zinc finger matrin-type protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000612">
        <property type="entry name" value="Zinc finger matrin-type protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052644">
        <property type="entry name" value="ZMAT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46786">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46786:SF1">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="12" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="U1-type" evidence="19">
        <location>
            <begin position="58"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="U1-type" evidence="19">
        <location>
            <begin position="131"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="U1-type" evidence="19">
        <location>
            <begin position="227"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="164"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00066226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00067763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="273" mass="30115" checksum="CC143C3FDFA51CAF" modified="2018-12-05" version="1">MDSLHHLGPPPIAATQPIRPPTLPPTQSQAAPAGEQPQDEGSLLGVEQDDPLGLEELCKPLYCKLCNVTLNSAQQAQAHYQGKNHSKKLRNFYAGSQQPPAIRIPEALEGAGPNAGSGALYKGAPRVILATENDYCKLCDASFSSLAVAQAHYQGKNHAKKLRLAETQQNSSSNEGAPRKNRKDGNEYRLVKNRRSPQLPAAMPGPYYNPRPRQRIPRDLAMCVTPSGQFYCSMCNCGAEQETDFRQHLESKQHKAKVSELRYRHEMENLGYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGC2</accession>
    <name>A0A3B5LGC2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGC2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008726"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008832.1">
        <property type="protein sequence ID" value="ENSXCOP00000008726.1"/>
        <property type="gene ID" value="ENSXCOG00000006667.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165756"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005162">
        <property type="entry name" value="Retrotrans_gag_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33198:SF19">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03732">
        <property type="entry name" value="Retrotrans_gag"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retrotransposon gag" evidence="1">
        <location>
            <begin position="57"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="219" mass="24860" checksum="051FDF5D4346A003" modified="2018-12-05" version="1">MAMHVGRVVEYDESKEDFESYLERLEQWMLANDVPDEKVVCTFLSVIGADTYGLLKNLVSPKVPSTMQYAALTAALQAHYKPTPVVIAERFRFQKRNQKEGEPVSDYVVALRQLSATCNFGQYLDEALRDRFVSGLRSDAAQRKLLAEKDLTFSRACEIAVNMELASKNTMEFSGHHDPHQVNALTNVPIFSKRGWKGHYTSLCSPCCPLCDRRDIEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGH2</accession>
    <name>A0A3B5LGH2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BACK domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGH2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008776"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008882.1">
        <property type="protein sequence ID" value="ENSXCOP00000008776.1"/>
        <property type="gene ID" value="ENSXCOG00000006702.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF14">
        <property type="entry name" value="KELCH-LIKE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21536">
        <property type="entry name" value="BTB_KLHL33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BACK" evidence="3">
        <location>
            <begin position="75"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="518" mass="57617" checksum="C6A12113F393633D" modified="2025-10-08" version="2">MLCGGMRESREDTVCLRGLSSWVIEAVVGFFYTGQLGLSWARVWELTDALHQFQLKGALSLCTDFLQGGMDESTCLDVLVLAETFGLVLLGQAAEEYVLTHFRLVSAEEKFKDIPFVLLDRLLDKDSLCVDREIVVFRAVVSWVEEEPKERLPFLSSLLSHVRLPLFTSSEVQEALRCKLLYRSSKTRAALETLQRLAKGEKVGSGRKPRSPNQASVLVLVGGDAVDDDFMKRVPIRSLWYAQQFHRGPGLMRNIEWKLLTKIPEPPRFRHAVCLFNNKLYILGGRKYYGSLDVLKSQLPDMLCPRDYFAAVCLDAKVFVLGGNYDDSQYTDDVQFYSPEKNTWRSARPLAAAVCGHAAAVLDRQIYVSGGCDSYHHCSSLLWTYHPSRGCFPRSPMSVGGGRAGHVMLAVGGRLVVAGGVQPLRVGFGDQLLCEVYDPACDSWSSFPALPRPHLSPGGTVLDGRLYVVGGSSANTARDTKWVYCYDPKKECWENLGAMPHPYTDLAVCALPLPNTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJ60</accession>
    <name>A0A3B5LJ60_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">L-amino-acid oxidase</fullName>
            <ecNumber evidence="4">1.4.3.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the flavin monoamine oxidase family. FIG1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.2" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJ60"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007914"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008012.1">
        <property type="protein sequence ID" value="ENSXCOP00000007914.1"/>
        <property type="gene ID" value="ENSXCOG00000006074.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160928"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001716">
        <property type="term" value="F:L-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.405.10:FF:000004">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000450">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF342">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Amine oxidase" evidence="11">
        <location>
            <begin position="56"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="245" mass="27450" checksum="D9531A6734D4B836" modified="2018-12-05" version="1">GKGFELFMSLFVSSAGSNPLYECLQDADYRELLDIVTDGLPAARMPQHVAVIGGGMAGLTAAKVLEDAGHKVTIIEASERIGGRVETFRNRREGWYAEMGAMRIPSFHKILLSFISKLKIPLNSFIQDDINTYYLVNGGLHKTYTVENNPSVLNYSLHDREKGKSAAELFSQTLWKVRDDLKTLGCSGMLKKYDSYTVKEYLVKEGNLSRGALRMIGDILNENSLFYTSLIEMLYIQTDINDNTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKF7</accession>
    <name>A0A3B5LKF7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Potassium channel, subfamily K, member 13b</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027895332.1">
        <property type="nucleotide sequence ID" value="XM_028039531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKF7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011140"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011268.1">
        <property type="protein sequence ID" value="ENSXCOP00000011140.1"/>
        <property type="gene ID" value="ENSXCOG00000008419.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114158206"/>
    <dbReference type="KEGG" id="xco:114158206"/>
    <dbReference type="CTD" id="556073"/>
    <dbReference type="GeneTree" id="ENSGT00940000157960"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000070">
        <property type="entry name" value="Potassium channel, subfamily K, member 12 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005410">
        <property type="entry name" value="2pore_dom_K_chnl_THIK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF314">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K, MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01588">
        <property type="entry name" value="THIKCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0630">Potassium</keyword>
    <keyword evidence="8" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="129"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="18">
        <location>
            <begin position="94"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="18">
        <location>
            <begin position="217"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="428" mass="47356" checksum="E8DDFF765889DC5C" modified="2018-12-05" version="1">MACRSGCCCGSSGPINEDNARFLLLALFIVLYLLCGAAVFSALEQPKEREAKARWAQRFEHFSQKHNLSRRDLRDFLRSYEEANVAGIRVDTVRPRWDFTGAFYFVGTVVSTIGFGMTTPATIGGKIFLMFYGLLGCAATILFFNLFLERVITVIAFVLKSFHERRRNKAMLPQNGRRASDGEKAGEAEAGGVGKREALAGWKPSVYCVMFILGAAAILVSCCASLMYSAAEGWGYLDSLYFCFVAFSTIGFGDMVSSQRISYEGHATAAYRVGNFFFILTGVCCIYSLFNVISIVIKQVLNWLLRRLEASCHCCFYRKGPHPHRHPRRNVVAPGHLRARRDPSIETDAINESETDPGRRMSGEMISMRDFLAANKVNLAIMQKQLSEMAVGHPRQSGGSCSHQNGFSGGVGALGIMNNRLAETSVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKL2</accession>
    <name>A0A3B5LKL2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKL2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010250.1">
        <property type="protein sequence ID" value="ENSXCOP00000010131.1"/>
        <property type="gene ID" value="ENSXCOG00000007686.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162818"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01504">
        <property type="entry name" value="PNCREATITSAP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="27"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="149" mass="16963" checksum="C2C515F8CAA965B8" modified="2018-12-05" version="1">YHVTVMITLGLLTTMYIYFLDKIRIVSDNSKLHFVNRYQTWARAERDCLSLGAQLASVHSFLEHHQIQNLISESGGGNQETWIGASDAEENSIWFWSDGRIFQFTNWCGGQPNNAGGKQSCVVMNYSVNTCWNDEQCTASRPYVCVKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKZ7</accession>
    <name>A0A3B5LKZ7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tribbles homolog 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Interacts with MAPK kinases and regulates activation of MAP kinases. Does not display kinase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027896592.1">
        <property type="nucleotide sequence ID" value="XM_028040791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKZ7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010241"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010364.1">
        <property type="protein sequence ID" value="ENSXCOP00000010241.1"/>
        <property type="gene ID" value="ENSXCOG00000007774.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114158889"/>
    <dbReference type="KEGG" id="xco:114158889"/>
    <dbReference type="CTD" id="28951"/>
    <dbReference type="GeneTree" id="ENSGT00950000182986"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031434">
        <property type="term" value="F:mitogen-activated protein kinase kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000153">
        <property type="entry name" value="Tribbles homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000253">
        <property type="entry name" value="tribbles homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024104">
        <property type="entry name" value="Tribbles/Ser_Thr_kinase_40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22961">
        <property type="entry name" value="SER/THR PROTEIN KINASE-TRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22961:SF15">
        <property type="entry name" value="TRIBBLES HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="61"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="344" mass="39055" checksum="F114D56E5D85C985" modified="2018-12-05" version="1">MNIQRSNPINISRYGRSRHKSHDFEELSCLRTTESNQSFSPNLGSPSPPETPDSSHCISRIGDYLLLEPLEGDHVFRAAHLHSGDELICKVFDIGRYQESLAAYFALGQHEHINQILEILLGETRAYVFFDRSHGDMHSFVRTCKKLREDEAARLFYQIASAVAHCHDNGLVLRDLKLRKFVFKNEDRNLVKLESLEDSYILAGLDDSLSDKHGCPAYVSPEILNANGSYSGKAADVWSLGVMLYTILVGRYPFHDVEPGSLFSKIRRGHFNIPETLTPKAKCLIRSILRREPAERLTSREILEHPWFASSGVLGGFQAHGRGEREQEQMVPEVNMEEELELFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLA3</accession>
    <name>A0A3B5LLA3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Charged multivesicular body protein 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027855923.1">
        <property type="nucleotide sequence ID" value="XM_028000122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLA3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011707"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011840.1">
        <property type="protein sequence ID" value="ENSXCOP00000011707.1"/>
        <property type="gene ID" value="ENSXCOG00000008838.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114133957"/>
    <dbReference type="KEGG" id="xco:114133957"/>
    <dbReference type="CTD" id="51652"/>
    <dbReference type="GeneTree" id="ENSGT00950000182832"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="180"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="20"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="221" mass="24725" checksum="E23C6307495463A8" modified="2018-12-05" version="1">MGLFGRTPDKPPKELINEWSQKIRKEMRVIDRQIRDIQREEEKVKRSIKDAAKKGQKDVCVILAKEMIQSKRAVTKLYASKAQMNSVLLSMKNQLAVVRVAGALQKSTEVMKVMQNLVKIPEIQATMMELSKEMMKAGIIEEMLEDTFESMEDGEEMEEAAEEEVDKILFEITAGALGKAPSKVTEPLPDLQPPGASAAASDEESEEDIEAMQSRLAALRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLE5</accession>
    <name>A0A3B5LLE5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLE5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013068.1">
        <property type="protein sequence ID" value="ENSXCOP00000012908.1"/>
        <property type="gene ID" value="ENSXCOG00000009768.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161979"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000965">
        <property type="entry name" value="Neurotrophin receptor-interacting factor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001480">
        <property type="entry name" value="Si:cabz01071911.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002169">
        <property type="entry name" value="Zgc:174573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000688">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001156">
        <property type="entry name" value="Zinc finger protein 407"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000303">
        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050826">
        <property type="entry name" value="Krueppel_C2H2_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24377">
        <property type="entry name" value="IP01015P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="117"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="206"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="234"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="262"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="290"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="79"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="316"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="316"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="408" mass="47258" checksum="569FB1448CA543DD" modified="2018-12-05" version="1">ASPSSSAGTRRIHTEEKRYSCSVCDQTFTWKRSLTQHMKKHSEEKHFRCSVCDETFISKAYLKKHSKIHKEKTLDYLKSSEKDGSGENLEESSEPQSALGSGKNKRDPVHKKGKKLHRCSECSKTFSCRQYLLKHNRIHTGEKPFSCSNCDKTFTWKPSLTLHMKKHSEENPFRCSVCDETFTRKLSLTLHMRTHSEEKQSEEKPFSCSVCSKTFGSKANFKNHAKVHTLHRPFKCLACYATFKRKSNLIEHMRKHTGEKRYSCSVCDQTFTWRNCLTRHLKKHSEEKHFRCSVCDETFISQANLKKHSRIHKEKTFDSLKSSEKDGSGENLEKRNESQSALGSGKSKTDPVYRKGKKLYLMAESVVVNQLQHSREQVCMRMIDSAKTLLLAWGKAREAKFFHRLSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLN4</accession>
    <name>A0A3B5LLN4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosducin b</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosducin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027883340.1">
        <property type="nucleotide sequence ID" value="XM_028027539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLN4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011615"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011747.1">
        <property type="protein sequence ID" value="ENSXCOP00000011615.1"/>
        <property type="gene ID" value="ENSXCOG00000008768.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114150822"/>
    <dbReference type="KEGG" id="xco:114150822"/>
    <dbReference type="CTD" id="386614"/>
    <dbReference type="GeneTree" id="ENSGT00940000156236"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008277">
        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02987">
        <property type="entry name" value="Phd_like_Phd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.168.10">
        <property type="entry name" value="Phosducin, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001200">
        <property type="entry name" value="Phosducin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051499">
        <property type="entry name" value="Phosducin-like_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023196">
        <property type="entry name" value="Phosducin_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024253">
        <property type="entry name" value="Phosducin_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46052:SF2">
        <property type="entry name" value="PHOSDUCIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46052">
        <property type="entry name" value="PHOSDUCIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02114">
        <property type="entry name" value="Phosducin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00677">
        <property type="entry name" value="PHOSDUCIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="3" id="KW-0844">Vision</keyword>
    <feature type="domain" description="Phosducin" evidence="5">
        <location>
            <begin position="7"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="236" mass="26921" checksum="43462A2087659AA4" modified="2018-12-05" version="1">MSGRVIDLEETATHTGPKGVINDWRRFKLESMDRENLPPAKKELLRQMSSPNKSKDDSRANLNRKMSVQEYELLKEEDEGCLKKYRRRCMEEMHSKLSFGPKFEGVHDLDSGEAFLEVIEKEHHSTVVVVHIYKDGVKGCEQLNSCLDCLATEYPTVKFCKIDAVSSGAAERFSDEYLPTLLVYKAGELIGNFLSCTQHMNEEFFATDVEAFLNSYGLLPEKELAGDNEEEEHDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LM19</accession>
    <name>A0A3B5LM19_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LM19"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010695.1">
        <property type="protein sequence ID" value="ENSXCOP00000010571.1"/>
        <property type="gene ID" value="ENSXCOG00000007994.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183052"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000508">
        <property type="entry name" value="Myeloid zinc finger 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000555">
        <property type="entry name" value="Zinc finger protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001325">
        <property type="entry name" value="zinc finger protein 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001465">
        <property type="entry name" value="Zinc finger protein 560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000030">
        <property type="entry name" value="Zinc finger protein 628"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050758">
        <property type="entry name" value="Znf_C2H2-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234:SF9">
        <property type="entry name" value="ZINC FINGER PROTEIN 850-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="225"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="255"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="311"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="339"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="367"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="395"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="423"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="451"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="479"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="507"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="535"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="563"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="25"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="592" mass="67299" checksum="8C75B80E7C428AEA" modified="2018-12-05" version="1">MSVNVWVVAGDRLLIQRESNSVRDRNLPTNLEMKEEHRDPDLNQMKENQNESVEHSKVKKLKKNMRSCKICGKHLIRKTLLKIHMRIHTGEKPHTCQVCSKSFSFSSHLVRHMRNHTGERPFSCQTCGKSYINRSSLNKPFSCEVCGDFFIDKLAVVKHMRSHTGEKPFPCDLCPKFFHTKFNLACHMRTHTGEKPYPCDLCDKSFISRGNLDRHRRSHTGEKPFSCEECGKLFTNKISSHLAEHTRSHSGEKPFQCLSCGKAFTKQNNLAVHIRTHTGEKPFSCPTCGKGFTQKGNLSIHMATHTGEKPFSCPTCGKGFTQKSNLHIHMRTHSGEKMFPCELCSKSFSFGCQLATHMRTHTGEKPFLCVACGKRFSQQNYLTVHMKIHTGERPFSCPTCGKSFIKEWNLSVHIRCHTGEKPYPCEVCGKSFINKSHLTNHMRTHTCEKPFSCLTCGKSFTKQGNLTDHIRSHTGEKPYPCKVCGKSFVNKSHLTNHLRTHTGEKPYDCKLCGKSFTDCSSIAQHMKTHTGEKPYPCDVCGKSFRNRNHLACHIRTHTGEKPFSCPICRKSFTQKGNLSIHVKTHTGENTQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LM76</accession>
    <name>A0A3B5LM76_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">N-acetylglucosamine-6-phosphate deacetylase</fullName>
            <ecNumber evidence="3 9">3.5.1.25</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>N-acetyl-D-glucosamine 6-phosphate + H2O = D-glucosamine 6-phosphate + acetate</text>
            <dbReference type="Rhea" id="RHEA:22936"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="3.5.1.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="12">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-sugar metabolism; N-acetylneuraminate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the metallo-dependent hydrolases superfamily. NagA family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.25" evidence="3 9"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LM76"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011820"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011956.1">
        <property type="protein sequence ID" value="ENSXCOP00000011820.1"/>
        <property type="gene ID" value="ENSXCOG00000008939.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012605"/>
    <dbReference type="UniPathway" id="UPA00629"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008448">
        <property type="term" value="F:N-acetylglucosamine-6-phosphate deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006046">
        <property type="term" value="P:N-acetylglucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019262">
        <property type="term" value="P:N-acetylneuraminate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106279">
        <property type="term" value="P:negative regulation of UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00854">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003764">
        <property type="entry name" value="GlcNAc_6-P_deAcase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113:SF14">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038994">
        <property type="entry name" value="NagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="13">
        <location>
            <begin position="60"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="233"/>
            <end position="234"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="326"/>
            <end position="328"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR038994-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="370" mass="39368" checksum="90642B29384B0F58" modified="2025-10-08" version="2">MGGGFGAGTAGCLQTTGNLDVLCLCREDLWVRDGKILDPEKLFFDEQGYADCRLDCGGAIVAPGFIDVQINGGYGVDFSQPCEDVSAGVSLVAKKILEHGVTSFCPTLVTSPPAVYHQVLPQINVHNGGPDGAGVLGFHLEGPFISSEKRGAHPERFLRTFRSGGSDDLMETYGSLDNVAMVTLAPELPHSQSVVSTLRQKGVTVSLGHSVANLSQAEDAVRHGASFITHLFNAMLPFHHRDPGIVGLLTSDQIPAGRTVFYGMIADGIHTNPAALRIAHRAHPSGLVLVTDAITAMGLPPGRHTLGQQVVQIQGLHAYVAGTKTLSGSIATMDMCVRHFKQAADLVLLDDGLRVRTTYISGEEVWRAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMM3</accession>
    <name>A0A3B5LMM3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fatty acid amide hydrolase 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027894637.1">
        <property type="nucleotide sequence ID" value="XM_028038836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMM3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011970"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012108.1">
        <property type="protein sequence ID" value="ENSXCOP00000011970.1"/>
        <property type="gene ID" value="ENSXCOG00000009062.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114157703"/>
    <dbReference type="KEGG" id="xco:114157703"/>
    <dbReference type="CTD" id="768298"/>
    <dbReference type="GeneTree" id="ENSGT00940000162502"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052739">
        <property type="entry name" value="FAAH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43372">
        <property type="entry name" value="FATTY-ACID AMIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43372:SF4">
        <property type="entry name" value="FATTY-ACID AMIDE HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="3">
        <location>
            <begin position="66"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="2">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="527" mass="57615" checksum="10D83D4ECD9A925C" modified="2018-12-05" version="1">MALSRLEKAQAWLFRAVTSFFFMIFQLLSSPAASARKLPPVSNPLLLQSATQLAKKIRRKEVSSVEVVQAYIDRIQEVNPFVNAVVKDRFAAALQEAAQVDKLIEEETGGEDVLEDRLPLLGVPLSVKESYSLQGMPLSTGLVSRRGVVASVDAPPVALLKRAGAVPLGVTNTSELCMWLESHNHLYGMTCNPYDLERIAGGSSGGEGSILGAAGAVIGVGSDIGGSIRIPCFFNGVFGHKTTPGVVSCENQYPPTSGTQHEYLSSGPMCRYAEDLLPLLKIMAGPKAYKLSLNADVDLKKLRFFSIPHDGGSLYTYPVSKELLDAQEKVVKRLEADLGVKVQEVCFPELRYGFQIWDTYMGLPDEEGKPPLAFAELMGEPGRPAWAFWELLKRMVGKSEHTMPAIFLALFEKTHLSKPSPFIIQQKEALQKKMEELLGTDGVLLYPPHPRVAPKHHHPLFRPFDFTYTGILNILGLPVTQCPLGLGEEGLPLGVQVVAGKLQDHLTLAVALYLEKTFGGWRDPGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNE8</accession>
    <name>A0A3B5LNE8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutamate receptor, ionotropic, delta 2 (Grid2) interacting protein, a</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNE8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013618"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013785.1">
        <property type="protein sequence ID" value="ENSXCOP00000013618.1"/>
        <property type="gene ID" value="ENSXCOG00000010313.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157625"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd07355">
        <property type="entry name" value="HN_L-delphilin-R2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06744">
        <property type="entry name" value="PDZ2_L-delphilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051425">
        <property type="entry name" value="Formin_Homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725:SF13">
        <property type="entry name" value="DELPHILIN-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725">
        <property type="entry name" value="FORMIN HOMOLOGY 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="90"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="3">
        <location>
            <begin position="535"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="913"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="481"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="515"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="933" mass="103722" checksum="66812402AB5EFF63" modified="2018-12-05" version="1">MSCAVIFIPKKHRERFDEVVSQSLMSRLKGRSFSDPSRSHLRRSQSEDHPERLLVSTRASSVPRTHAEEGVVPPARGMRKTTSLIAGHSGVRVCKGNMSFGFTLRGHAPVWIDSVIPGSPADKAGLKPGDRILFLNGLDMRTSSHEKVVSMLQGSGAMPTLVVEEGPPTFPLAEQDLAGGSVPTERARSPVLSSLQWVADILPPSIRVQGRTFGQQLEHLLTIQERYTICKALENFFQHRNVDTLIVDVFPVLDTPAKQVIWQFVYQLLTYEEQEHCQNKISRFLGYKAPGLCTFIATINDSGMRLAPGERQSGDGTSLPETPNNLTNLSFVSGARKGSSPHPSWPDPLPSPPSGQFYPPGLTSQTSGESNPYISLDSPPPSPLEPDFPCSPPVHRNNKRRYTFSKPPRSEDTDRFLDALSEQLGQRVAIVDDFLTPDNDYEEEENGGFVAPELSSPSDVQSSSGEENASSLTYSSSSDHIPPPPMTPPPPPPVQFNDPPPPPPPHMPRAYNLGLPPPPPLPPPCSPPPLPRPTLSRMDSHHMSVKRLRWEQVENSEGTIWGQLGETSDYEKLHNMVKYLDLELHFGTQKGSLPVPEPLPHLETFKKKDVIEILSHKKAYNASILIAHLKLSPGELRQVLMNMAADRLEAAHIKQLLLYAPDAEEVKKYEAYREDPSKLSEPDQFMLQMLSVPEYNTRLQSLLFKCSLQEKTEELRGAFDCIIKASMELKTSKKLAKILEFVLAMGNYLNNSQPKTNKTTGFKINFLTELSTTKTVDGKSTFLHILVKSLCQHFPEVLDFSKDLNMVPLATKVNQRTITSDVSDIHTAIQDIRSACQKMPATAEDRFAVVMSNFLENSHPAIQSLESLQQRAMEEFSKTASYFGEDGKATTTEAFFGIFAEFMNKFERALSDQQAAEIPKSPRSPRMASPQAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNP0</accession>
    <name>A0A3B5LNP0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.7.19" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNP0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009464"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009575.1">
        <property type="protein sequence ID" value="ENSXCOP00000009464.1"/>
        <property type="gene ID" value="ENSXCOG00000007209.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156988"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045100">
        <property type="entry name" value="TUT4/7_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271">
        <property type="entry name" value="POLY A POLYMERASE CID PAP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271:SF40">
        <property type="entry name" value="POLY(A) RNA POLYMERASE GLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19088">
        <property type="entry name" value="TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Terminal uridylyltransferase 4/7 nucleotidyltransferase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="144" mass="16337" checksum="04692D6B57455713" modified="2018-12-05" version="1">MTQPEVLIQVLGILKNRPEYSEVESDFHSKVPAVFCRDRSSGLICKVSAGNDVACLTTNHLAALAKLEPRLVPLVLAFRYWARLCHIDCQAEGGIPSYSFALMVIFFLQQRSDPVLPVYLGSWVGESQKYWILFNITDRKKNNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNX0</accession>
    <name>A0A3B5LNX0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRIB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BORG/CEP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027855330.1">
        <property type="nucleotide sequence ID" value="XM_027999529.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027855332.1">
        <property type="nucleotide sequence ID" value="XM_027999531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNX0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012717"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012871.1">
        <property type="protein sequence ID" value="ENSXCOP00000012717.1"/>
        <property type="gene ID" value="ENSXCOG00000009610.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114133535"/>
    <dbReference type="KEGG" id="xco:114133535"/>
    <dbReference type="GeneTree" id="ENSGT00940000165053"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030838">
        <property type="term" value="P:positive regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031274">
        <property type="term" value="P:positive regulation of pseudopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029273">
        <property type="entry name" value="Cdc42_effect-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051296">
        <property type="entry name" value="Cdc42_Effector_BORG/CEP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15344:SF2">
        <property type="entry name" value="CDC42 EFFECTOR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15344">
        <property type="entry name" value="CDC42 EFFECTOR PROTEIN BORG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14957">
        <property type="entry name" value="BORG_CEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIB" evidence="4">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="117"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="245" mass="26149" checksum="22794D9F298AB65C" modified="2018-12-05" version="1">MPAKTPIYLKTTTPKKGKRLRLRDVLSGDMISPPLGDVRHSAHVGPEGKGDMFGDVGFLQGKMDMLPGHGHARSHSVDQAATQEPRSFAYNGCHYQQASNGLLKSTVSMPVCVAHEQAPPKPPRLHLDQQPGEPGDAQPEVCEASAVRGLVPSSGSFSEASSEDSVSETCGPPDVRRGLSLDSDAGLSNEDLSSERSDSPCPVPGLQPADSLARLDLDLDLGPSILEDVLSIMDRYKAEDDRCEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPL1</accession>
    <name>A0A3B5LPL1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">2-oxoisovalerate dehydrogenase subunit alpha</fullName>
            <ecNumber evidence="12">1.2.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="function">
        <text evidence="9">Together with BCKDHB forms the heterotetrameric E1 subunit of the mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. The BCKD complex catalyzes the multi-step oxidative decarboxylation of alpha-ketoacids derived from the branched-chain amino-acids valine, leucine and isoleucine producing CO2 and acyl-CoA which is subsequently utilized to produce energy. The E1 subunit catalyzes the first step with the decarboxylation of the alpha-ketoacid forming an enzyme-product intermediate. A reductive acylation mediated by the lipoylamide cofactor of E2 extracts the acyl group from the E1 active site for the next step of the reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 3-methyl-2-oxobutanoate + H(+) = N(6)-[(R)-S(8)-2-methylpropanoyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:13457"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10497"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83142"/>
            <dbReference type="EC" id="1.2.4.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:13458"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterotetramer of 2 alpha/BCKDHA and 2 beta chains/BCKDHB that forms the branched-chain alpha-keto acid decarboxylase (E1) component of the BCKD complex. The branched-chain alpha-ketoacid dehydrogenase is a large complex composed of three major building blocks E1, E2 and E3. It is organized around E2, a 24-meric cubic core composed of DBT, to which are associated 6 to 12 copies of E1, and approximately 6 copies of the dehydrogenase E3, a DLD dimer. Interacts with PPM1K.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the BCKDHA family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.4" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPL1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011376"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011508.1">
        <property type="protein sequence ID" value="ENSXCOP00000011376.1"/>
        <property type="gene ID" value="ENSXCOG00000008596.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063174"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003863">
        <property type="term" value="F:branched-chain 2-oxo acid dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000015">
        <property type="entry name" value="2-oxoisovalerate dehydrogenase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050771">
        <property type="entry name" value="Alpha-ketoacid_DH_E1_comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43380:SF1">
        <property type="entry name" value="2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="13">
        <location>
            <begin position="109"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="447" mass="50991" checksum="B82463DC62ADDF37" modified="2018-12-05" version="1">MAAVSGMSRIYGLCRHAVRQTAALKTPRLLQRRSFIVTAVQQQQQPFDSSLEKPQFPGASAEFVDRLEFIQPNVISGIPVYRVMDRQGNIINPSEDPQLSKETVLNFYQKMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHIGSAAALESNDLVFGQYREAGVLMYRGFPLDLFMAQCYANADDLGKGRQMPVHYGCKDLHFVTISSPLATQIPQAAGAAYALKRENMNRAVICYFGEGAASEGDAHAGFNFSATLECPLIFFCRNNGYAISTPTNEQYRGDGIAARGPGYGMLSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVNYWDKQDHPISRLRHYMTARGWWSEDDERSWRNQSRKTVMEAFEKAERRLKPNPELLFTDVYQEMNPTLAKQRESLFRHIQQYKEHYPLDLYEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQ94</accession>
    <name>A0A3B5LQ94_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromatin-remodeling ATPase INO80</fullName>
            <ecNumber evidence="4">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">ATPase component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the INO80 chromatin-remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">The DBINO region is involved in binding to DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQ94"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009949"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010067.1">
        <property type="protein sequence ID" value="ENSXCOP00000009949.1"/>
        <property type="gene ID" value="ENSXCOG00000007547.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00900000141110"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF2">
        <property type="entry name" value="CHROMATIN-REMODELING ATPASE INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="72"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="149"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="189"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU368001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="224" mass="25021" checksum="0500F18DC304362E" modified="2018-12-05" version="1">MDRAHRLGQTKQVTVYRLICQGTIEERILQRAKEKSEIQRVVISGGNFKPDTLKPKEVVSLLLDDDELEKKLRQRQEEKRQQEESSKVKERKRKREKYAEKQKKNEESENKRKKEMNLVISHAPSADNSNLSADGDDSFISVEMDSAMPSPFSEISLSSELQPGSLPPDADADESSSDMLVIVDDPVSSAPQSRATNSPASVTGSVSDNMNGEESKYGVLAVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQG8</accession>
    <name>A0A3B5LQG8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sodium/potassium-transporting ATPase subunit beta-1-interacting protein</fullName>
            <shortName evidence="7">Na(+)/K(+)-transporting ATPase subunit beta-1-interacting protein</shortName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NKAIN family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQG8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010024"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010142.1">
        <property type="protein sequence ID" value="ENSXCOP00000010024.1"/>
        <property type="gene ID" value="ENSXCOG00000007605.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160565"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002028">
        <property type="term" value="P:regulation of sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008516">
        <property type="entry name" value="Na/K-Atpase_Interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13084:SF3">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT BETA-1-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13084">
        <property type="entry name" value="T-CELL LYMPHOMA BREAKPOINT-ASSOCIATED TARGET 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05640">
        <property type="entry name" value="NKAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="189" mass="21722" checksum="67CA62E64CE88356" modified="2018-12-05" version="1">MGCCSGRCTLAFICGMQLVSVLERQVIDFLGYQWAPILTNFLHIIVVILGLFGTIQFRPKYYAAWLVIWMTWNVFIICFYLEVGDLSRSDLVLTFNLSMHRSWWMENGPGCRVTPITPPPSWAPEDHRYISISDCLMNFEFIEVAHSSLQILLALAGFIYACYVVKLISEEEDSCNFGFVTSHIWELKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LS18</accession>
    <name>A0A3B5LS18_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Pyrroline-5-carboxylate reductase</fullName>
            <ecNumber evidence="4 15">1.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-proline + NAD(+) = (S)-1-pyrroline-5-carboxylate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:14107"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-proline + NADP(+) = (S)-1-pyrroline-5-carboxylate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:14111"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="4 15"/>
    <dbReference type="RefSeq" id="XP_027898540.1">
        <property type="nucleotide sequence ID" value="XM_028042739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LS18"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013867"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014037.1">
        <property type="protein sequence ID" value="ENSXCOP00000013867.1"/>
        <property type="gene ID" value="ENSXCOG00000010499.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114160249"/>
    <dbReference type="CTD" id="393799"/>
    <dbReference type="GeneTree" id="ENSGT00950000183044"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000064">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3730.10:FF:000003">
        <property type="entry name" value="Pyrroline-5-carboxylate reductase 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.10">
        <property type="entry name" value="ProC C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053790">
        <property type="entry name" value="P5CR-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645:SF63">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00521">
        <property type="entry name" value="P5CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 14 15" id="KW-0521">NADP</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 15" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="16">
        <location>
            <begin position="3"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase dimerisation" evidence="17">
        <location>
            <begin position="164"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="6"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="69"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="95"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="321" mass="34140" checksum="BCBEF09F58B68FAA" modified="2018-12-05" version="1">MSVGFIGAGQLAQALVRGFSVAGVIATHRITASSPDTELPTVQQLRKLGVNFTTSNKETVSRSDVLFLAVKPHIIPFVLDEIGPDIEDRHLIVSCAAGVTISSIEKKLQQYRTSPKVMRCMTNTPVVVREGATVYATGTHAEVEDGKLLEQLMASVGYCTEVEEDLIDAVTGLSGSGPAYAFLAVDALSDGGVKMGLPRRLAVRLGAQALLGAARMLLDSEQHPGQLKDNVCSPGGATIHALHVMESGGFRSLLINAVEASCVRTRELQFLADQEKISPAAIKKTTLDKVLQQPGVTADAVGVRTRGINIFNNTSPKIKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSG7</accession>
    <name>A0A3B5LSG7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="3">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSG7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010754"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010879.1">
        <property type="protein sequence ID" value="ENSXCOP00000010754.1"/>
        <property type="gene ID" value="ENSXCOG00000008118.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163892"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005005">
        <property type="term" value="F:transmembrane-ephrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000019">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000045">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1770:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1770">
        <property type="entry name" value="ephrin a2 ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027936">
        <property type="entry name" value="Eph_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001090">
        <property type="entry name" value="Ephrin_rcpt_lig-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050449">
        <property type="entry name" value="Ephrin_rcpt_TKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016257">
        <property type="entry name" value="Tyr_kinase_ephrin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001426">
        <property type="entry name" value="Tyr_kinase_rcpt_V_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877">
        <property type="entry name" value="EPH RECEPTOR A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877:SF18">
        <property type="entry name" value="EPHRIN TYPE-A RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14575">
        <property type="entry name" value="EphA2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25599">
        <property type="entry name" value="Ephrin_CRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01404">
        <property type="entry name" value="Ephrin_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000666">
        <property type="entry name" value="TyrPK_ephrin_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00615">
        <property type="entry name" value="EPH_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51550">
        <property type="entry name" value="EPH_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00790">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00791">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13 21 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="22" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="15 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 21 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="16" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="499"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Eph LBD" evidence="28">
        <location>
            <begin position="11"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="27">
        <location>
            <begin position="395"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="568"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="26">
        <location>
            <begin position="863"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="693"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="574"/>
            <end position="582"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21 23">
        <location>
            <position position="600"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="54"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="929" mass="103941" checksum="46DDE09D89EA1E71" modified="2018-12-05" version="1">LFTCVCVSAIPVTLLDTRTVPGELKWAANPAEGGWEEVSIMDEKNIPIRTYQVCNVLEPSQNNWLRTDWIPRSGAQRVYVEVKFTLRDCNSLPGVTGTCKETFNLYYHESNEDRESYIKESSFIKVDTVAADESFTQVDVGDRIMKLNTEIRDVKVTTRKGFYLAFQDVGACIALVSVRVFYKTCPLTVRNLATFPDTVTGADTSSLVEVRGSCVNQSEEREEPKMYCGADGEWLVPIGGCFCKPGYEEQGGACKACSAGFYKSKSSDPGCSKCPPHSRSLRDGANVCDCHTGFFRADFDPPSMACTRKKPPSAPQQLISPPRNHTLCLPCGSDVLFSPDQTGLQTTRMVVSRLRAHTHYTFIVYARNGVSLVSGAGASQSASVSLTTNQAAPSPVSSIQLTDVTRHSLSLAWQQPDRPNGVILEYEVKFYEKDQRERSYRIMRTFLRSVDVAGLNPLTVYVFHVRARTAAGYGEYSAPTCLLLSGDIAAKLARDSWEVIKLLVFVAGLFIQLVFLAFLFIHPSPPSFLVHFRLPSVVGVKIYVDPFTYEDPDQAIHEFAKEIDVSNIHIERVIGIGEFGEVCSGRLRSQGKREIYVAIKSLKAGYSDKQRRDFLSEASIMGQFDHPNIIRLEGVVTRCKPLMIITEYMENGSLDAFLRKHDGQFTVIQLVGMLRGVASGMKYLSDMSYVHRDLAARNILVNSNLVCKVSDFGLSRVLEDDPEAAYTTRVRLIPIRWTAPEAISYRKFTTASDVWSYGIVMWEVVSYGERPYWDMNNQDVIKAIEEGYRLPAPMDCPVVLHQLMLDCWERERGDRPSFSQILNMLDKLIRNPGTLPPGTMLESRVGSEVCVSVVPEVCVPPWSMYEWLQSIGLERYRDVLAAAGYSSLESLLMDRLGIITPSHQDILIASVQQEMLSQMQHMQHSMVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTW4</accession>
    <name>A0A3B5LTW4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTW4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014290"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014467.1">
        <property type="protein sequence ID" value="ENSXCOP00000014290.1"/>
        <property type="gene ID" value="ENSXCOG00000010831.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155751"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF54">
        <property type="entry name" value="EPIDERMAL GROWTH FACTOR RECEPTOR SUBSTRATE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EH" evidence="1">
        <location>
            <begin position="12"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="95" mass="10242" checksum="B36BE325B7264B3A" modified="2018-12-05" version="1">MYLNTTCSCVFQVDPTGSGRVAAADAALFLKQSGLADMVLGKIWDLADIERKGFLNKQQFFIALRLVACAQNGLEVALKSLNVAVPPPKFVSLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUZ7</accession>
    <name>A0A3B5LUZ7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cyclin-dependent kinase inhibitor 2A/B (p15, inhibits CDK4)</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027873615.1">
        <property type="nucleotide sequence ID" value="XM_028017814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUZ7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014962"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015150.1">
        <property type="protein sequence ID" value="ENSXCOP00000014962.1"/>
        <property type="gene ID" value="ENSXCOG00000011301.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114144722"/>
    <dbReference type="KEGG" id="xco:114144722"/>
    <dbReference type="CTD" id="100329528"/>
    <dbReference type="GeneTree" id="ENSGT00940000159801"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000045">
        <property type="term" value="P:regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050776">
        <property type="entry name" value="Ank_Repeat/CDKN_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24201:SF8">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 4 INHIBITOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="68"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="124" mass="13048" checksum="47F35C133707BD3F" modified="2018-12-05" version="1">MTLEDELTTAAAKGHTAEVEALLLQGAPVNGVNSFGRRAIQVMMMGSSEVARLLLTRGADPNVTDKSTGATPLHDAARTGFLDTVQLLVKAGADPQARDKDNCLPIDLARQNGHTDVVAVLETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWG6</accession>
    <name>A0A3B5LWG6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">BCL2 interacting protein 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SEC20 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWG6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013666"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013834.1">
        <property type="protein sequence ID" value="ENSXCOP00000013666.1"/>
        <property type="gene ID" value="ENSXCOG00000010349.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014412"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15865">
        <property type="entry name" value="SNARE_SEC20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005606">
        <property type="entry name" value="Sec20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056173">
        <property type="entry name" value="Sec20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12825">
        <property type="entry name" value="BNIP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12825:SF0">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SEC20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03908">
        <property type="entry name" value="Sec20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Sec20 C-terminal" evidence="11">
        <location>
            <begin position="134"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="228" mass="26168" checksum="F71A7FCF695624D3" modified="2018-12-05" version="1">MATSDVHVRVCEQEILKYDLEIKALIQDITECGGPQSKLTEINTDVKKHFHNLRLRIQDFEWMAMEQDRESDKQALLSHVEGHRKQMLSNQTAWRKANLASKLSMDKMEKQALLNGADGAVRKRKMTKEGLAQSASDITENLMSISRMMAQQVQQSEDSITTLATSSRTIQDTNEEFKAMTGTIQLGRKLITKYNRRELTDKLLIFLALALFFATVLYILKKRLFPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWH5</accession>
    <name>A0A3B5LWH5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWH5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015674.1">
        <property type="protein sequence ID" value="ENSXCOP00000015482.1"/>
        <property type="gene ID" value="ENSXCOG00000011713.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287337"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045942645" description="C-type lectin domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="12650" checksum="D8520E3A69B0FF8D" modified="2025-10-08" version="2">MNSSSLCVCIILLLSNVIDLHFSKQTDLLQRSLSCPSGWTLINSRSEVISNNIRNCLSMGTNLASVQDLNEYNQIQIIVTAAGHGAKQAWIGGTNAQEVLLPVHFISRVVGLGQLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LX87</accession>
    <name>A0A3B5LX87_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LX87"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015767"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015963.1">
        <property type="protein sequence ID" value="ENSXCOP00000015767.1"/>
        <property type="gene ID" value="ENSXCOG00000011916.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="2">
        <location>
            <begin position="45"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="105" mass="11555" checksum="150D90E05F2B777E" modified="2018-12-05" version="1">PDAPPPRPARRQRRAPPVDPAPHRFPELTPPQPVKSGEVSSGSYSHSCQEFHRETQLPRDVDPQSVSCCLTEDGKLKFKAQRRALPAAAERVVVIEHVSPSSSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXN4</accession>
    <name>A0A3B5LXN4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Coiled-coil domain containing 191</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXN4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012735.1">
        <property type="protein sequence ID" value="ENSXCOP00000012584.1"/>
        <property type="gene ID" value="ENSXCOG00000009510.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154110"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052270">
        <property type="entry name" value="CACF_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22028:SF5">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 191"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22028">
        <property type="entry name" value="SFI1 SPINDLE BODY DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="574" mass="68487" checksum="DBD44F3F7B0510E6" modified="2018-12-05" version="1">MASELAVSEVFSHRKPQSAINRVAVPLESSEQLRDHDDAYSEGRSAHRKSYLLLLGSSLDLISDLYDCLVEEEEQLAVESFLQDLMKQELLDCGMLEELSRDDREKQKKFRNPAITMEARHQQVRENRAQREAEKQRKRREKESQQDVREEAKKKEQEGEKRKKQLKRREEEMVQQEMVRLRRLMEEKKALEHAARQNSLTGLGFQVVISPADVNAVVQLLQCLHKHLSGWHSVVLRRKLHMGKAVALCDWRRQLRAWRAWRAVVWAERSRREVARTAEQLRIENRQVKGSVLTFNATDKLHNTQLFAKVFWPLELFSMEVRARQRAERRQEIEEIKRRKEEEKLAGTRGEEYFMFYSPDTSLYKLHTRCKYCFSASIQREAERQRQLKRRQELMRLACQHHNKTLLSHRGLAPWKHLMQLRRENMELAESHHNLFLLKQCTLEWLQYARETQRERVACADQLQKHFLLRWSLNCWKRVSIPFYTQYTLPCKGFCRSLRSTSLMLTCSVFVCPSICLCFRRVLQRCFQAWRQLPSTLRREREREVRREKLSRKVAELLPDFCSRPLGMSERETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYN7</accession>
    <name>A0A3B5LYN7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dipeptidyl peptidase 1</fullName>
            <ecNumber evidence="6">3.4.14.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Cathepsin C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Cathepsin J</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Dipeptidyl peptidase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Dipeptidyl transferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Thiol protease. Has dipeptidylpeptidase activity. Active against a broad range of dipeptide substrates composed of both polar and hydrophobic amino acids. Proline cannot occupy the P1 position and arginine cannot occupy the P2 position of the substrate. Can act as both an exopeptidase and endopeptidase. Activates serine proteases such as elastase, cathepsin G and granzymes A and B.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, except when Xaa is Arg or Lys, or Yaa or Zaa is Pro.</text>
            <dbReference type="EC" id="3.4.14.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Tetramer of heterotrimers consisting of exclusion domain, heavy- and light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.1" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYN7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017008"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017227.1">
        <property type="protein sequence ID" value="ENSXCOP00000017008.1"/>
        <property type="gene ID" value="ENSXCOG00000012827.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155787"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02621">
        <property type="entry name" value="Peptidase_C1A_CathepsinC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.80:FF:000001">
        <property type="entry name" value="Dipeptidyl peptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000062">
        <property type="entry name" value="Dipeptidyl peptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.80">
        <property type="entry name" value="Cathepsin C, exclusion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039412">
        <property type="entry name" value="CatC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014882">
        <property type="entry name" value="CathepsinC_exc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036496">
        <property type="entry name" value="CathepsinC_exc_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025661">
        <property type="entry name" value="Pept_asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013128">
        <property type="entry name" value="Peptidase_C1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000668">
        <property type="entry name" value="Peptidase_C1A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12411">
        <property type="entry name" value="CYSTEINE PROTEASE FAMILY C1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08773">
        <property type="entry name" value="CathepsinC_exc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00112">
        <property type="entry name" value="Peptidase_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00705">
        <property type="entry name" value="PAPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00645">
        <property type="entry name" value="Pept_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75001">
        <property type="entry name" value="Dipeptidyl peptidase I (cathepsin C), exclusion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00640">
        <property type="entry name" value="THIOL_PROTEASE_ASN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0868">Chloride</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="21" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5018681356" description="Dipeptidyl peptidase 1" evidence="21">
        <location>
            <begin position="21"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C1A papain C-terminal" evidence="22">
        <location>
            <begin position="213"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00030778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00032961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00045556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="444" mass="48994" checksum="2E7E2D3E303D2CC2" modified="2018-12-05" version="1">MRFSAVLVCVFLLWVEGSLGDTPANCTYEDLLGTWVFQVSKGGHDKTVNCSVEGKNTVTVTLEKLSVATDELGTTGFFTLIYNQGFEVVLGGYKWFAFFKYTQEGSKVTSYCDQTLPGWVHDVLGRNWACFVGKRVKPVPPRADSKPPYKNNWDFIDLINTVQSSWKAVAYPEHEMYTLQELHYRAGGPASRIPVRVRPAPVEAGLAKMAAALPEHWDWRNVDGVSFVSPVRNQASCGSCYSFATMGMLEARVRILTNNSDSPVLSPQQVVSCSEYSQGCDGGFPYLIGKYVQDFGIVDESCFPYTAKDTPCGVPANCGKMYTAEYGYVGGFYGGCSETAMMLELVSNGPMAVALEVYPDFMAYKGGIYHHTGLADSINPFELTNHAVLLVGYGRCHKTGQKYWIVKNSWGAQWGEEGYFRIRRGSDECAIESIAVAAKPIPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYZ8</accession>
    <name>A0A3B5LYZ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sodium/glucose cotransporter 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">High affinity sodium-glucose cotransporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Solute carrier family 5 member 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>D-galactose(out) + 2 Na(+)(out) = D-galactose(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70499"/>
            <dbReference type="ChEBI" id="CHEBI:4139"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:70500"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>D-glucose(out) + 2 Na(+)(out) = D-glucose(in) + 2 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70495"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:70496"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYZ8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017113"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017333.1">
        <property type="protein sequence ID" value="ENSXCOP00000017113.1"/>
        <property type="gene ID" value="ENSXCOG00000012899.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155844"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005412">
        <property type="term" value="F:D-glucose:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018212">
        <property type="entry name" value="Na/solute_symporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819:SF151">
        <property type="entry name" value="SODIUM_GLUCOSE COTRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00456">
        <property type="entry name" value="NA_SOLUT_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="14" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="18"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="218"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="262"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="277"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00040046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="302" mass="33729" checksum="354863392F21871F" modified="2018-12-05" version="1">ADMFSGGIFIQQALGLNIYVAVIALLAITALYTVTGGLAAVIYTDALQTIIMVIGSFILMGFAFNKVGGYENFETLYMTAVPTNTTNISAECYEPRKDAFHLFRDPITGDLPWPGMVFGLTIQATWYWCADQVIVQRCLSAKNLSHVKAGCILCGYLKLLPMFLMVFPGMISRILYPDVVACVIPEECNKYCGAEVGCTNIAYPKLVVDLMPNGLRGLMLSVMMASLMSSLTSIFNSASTLFTMDIYTKVRPRAKERELMIAGRESTQSGRKRSVGMKREEQMETKRNQQDERWRRENFTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZ78</accession>
    <name>A0A3B5LZ78_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZ78"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017188"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017408.1">
        <property type="protein sequence ID" value="ENSXCOP00000017188.1"/>
        <property type="gene ID" value="ENSXCOG00000012958.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159161"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046620">
        <property type="term" value="P:regulation of organ growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21777">
        <property type="entry name" value="MobB_LATS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000086">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000199">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000170">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF149">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE LATS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="648"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="349"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="492"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1044"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="297"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="352"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="380"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="526"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="563"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="1055"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="677"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1081" mass="119411" checksum="A4EA9D96A9C33A65" modified="2018-12-05" version="1">MKSRIYSCVQHELSYFTVVVDFFYSWFQFGGLNFPLTPKEICQGVRALKQTGSRNIEAALEYISKMGYLDPRNELIVRVIKQTSPGKAGMPNGMDHRPALEVPGEGGALNPYHQMGAQMYDGAAGYGPESDLTRAYMGAPPVINYMMPPSSTAQGPAMGNPMGRPPSMGSYPPGMPTQSNPGSAMYPPGAPQKAYPGSMEQHGHMMSYNVPGQPLQLQPQPPGGPVPGPHYDYAHARQHMMEPAGYAVKRTPSFQNKMQPPPMGPPDNYVNMQAKGAMSQNGTAGGGYPANLYLSSHSHPRQASPTSHQVHMMSRSPGGVPPMGPEFSDLPQGMMTPSRASLNLDLYEHHWAGPPGADGTPAARQPQPQGPCRGEVRVPSRTNSFNSRSAAPNGVRPTMTAPPTAGKQDSSMGPPNTITAVTSPPIQQPVKSIRVMRPEPKTAVGPCHPGWMAAQAPDAAEPLPYMPEETYPLEPAQEQRCPPPPYPKNLLIPAGEPGVLEGGGASGPDLSNATARGGRSGSGGSRKAEESHQVKEKTKAGKGEKAVKDKKQIQTSPVPVRKNGRDEEKRESRIKTYSPFAFKFYMEQHIENVMKTYQQKLNRRLQLEQEMSKAGLSEAEQGQMRKMLNQKESNYNRLRRAKMDKSMFIKIKTLGIGAFGEVCLTRKVDTGALYAMKTLRKKDVLNRNQVAHVKAERDILAEADNEWVVRLYYSFQDRDSLYFVMDYIPGGDMMSLLIRMGVFPEHLARFYIAELTLAIESVHKMGFIHRDIKPDNILIDLDGHIKLTDFGLCTGFRWTHNSKYYQKGSHIRQDSMEPSDSWDDVSNCRCGDRLMTLEQRANRQHQRCLAHSLVGTPNYIAPEVLLRKGYTQLCDWWSVGVILFEMLVGQPPFLAPTPTETQIKVINWESTLQVPPQVKLSPESVDIIGRLCCSAEERLGANGSGEIKAHPFFSEVDFSSNLRQQLAPYRPKIAHPMDTSNFDPVEEEGVPGAWSDSGDSTRALDVLCSPHGKHPEHAFYEFTFRRFFDDHGCPFRYPKPPEADACLSVPGVGPEEGEETEEEEDDEEEGEGGEGCEPVYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M1B1</accession>
    <name>A0A3B5M1B1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027897041.1">
        <property type="nucleotide sequence ID" value="XM_028041240.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027897042.1">
        <property type="nucleotide sequence ID" value="XM_028041241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M1B1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013809"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013978.1">
        <property type="protein sequence ID" value="ENSXCOP00000013809.1"/>
        <property type="gene ID" value="ENSXCOG00000010455.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114159127"/>
    <dbReference type="KEGG" id="xco:114159127"/>
    <dbReference type="CTD" id="322617"/>
    <dbReference type="GeneTree" id="ENSGT01150000286930"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000001">
        <property type="entry name" value="Gap junction alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF109">
        <property type="entry name" value="CONNEXIN 28.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0303">Gap junction</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="194"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="12">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="13">
        <location>
            <begin position="151"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="283" mass="32151" checksum="633B6DE4AB8B3341" modified="2018-12-05" version="1">MGDWGILSTLLEQVQSHSTVIGKIWMSILFLFRIMVLGAGAESVWGDEQSDFICNTQQPGCENVCYDSTFPISHIRFWVLQILFVSTPTLIYLGHAMHVIQQEKKLREALASPDGPRKFKQPKYSDEKGHVKIKGNLLGSYLTQLIFKIIIEAGFIVGQYYLYGFVMVPMFPCQKKPCPFTVECYMSRPTEKTIFIIFMLVVACVSLLLNVIELVYLMATRCRCKRRTQRVTSAEHPASLSGTRWPTTEDTMRQNKVNMELENHSVAGSLDGAKEEKRLLSGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M1S9</accession>
    <name>A0A3B5M1S9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M1S9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018043"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018270.1">
        <property type="protein sequence ID" value="ENSXCOP00000018043.1"/>
        <property type="gene ID" value="ENSXCOG00000013594.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165343"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01852">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002274">
        <property type="entry name" value="Si:dkeyp-69e1.8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF167">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017479554" description="AIG1-type G domain-containing protein" evidence="4">
        <location>
            <begin position="25"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="AIG1-type G" evidence="5">
        <location>
            <begin position="6"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="256" mass="28136" checksum="81D29C7C932D90A9" modified="2018-12-05" version="1">MPNDFKFFVRLVLLGLSGSGKSAAGNTILGQAMFESNSESFTAATQKCEKKKAMVDGKRVSVVDTPDWFNSEQTPDEVRAQISSCIALSSPGPHAFLLCVPLDQPAKTELQALSALAKVFGPEAVQKHTMVLFTYADRLRESGKAGGKSVEEYIASQRGDLVKLVEKCTDRFHVMEKGQGWRQKKNVAELLEKVEQTVKEAGGQCYSSPAFQEVEDRVRQRQLEIVREKKGIKQETALQAGLVGAWRGGGGKKHYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M227</accession>
    <name>A0A3B5M227_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the interleukin-1 receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M227"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014079"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014254.1">
        <property type="protein sequence ID" value="ENSXCOP00000014079.1"/>
        <property type="gene ID" value="ENSXCOG00000010662.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01090000259985"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004908">
        <property type="term" value="F:interleukin-1 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015621">
        <property type="entry name" value="IL-1_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004074">
        <property type="entry name" value="IL-1_rcpt_I/II-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890:SF3">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01536">
        <property type="entry name" value="INTRLKN1R12F"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017434087" description="TIR domain-containing protein" evidence="9">
        <location>
            <begin position="27"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="348"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="10">
        <location>
            <begin position="395"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="494"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="635" mass="71966" checksum="AE95C47CEF6A4E06" modified="2018-12-05" version="1">MGPSSSPESFLMFLWISGICGGLAQAELMKKGWFCFLSCDKHMNKYMRSPVLDLTLLSPDVFDFTAVPFNITWFSAKTGQEMRNEAGRILVLQETLWFLNTTMDDDGEYLAIVRTPSQCYMQAAKLVMDRPVAGECGRPRKHPRDVVKDLQCPLGDYIHQLKIYGIDVSVNWYKFLFKTVDGAADKTRIYSAGFSEGSGAYTCTLTFALDGATRSVSETINATVTEQYSLSPQIHEPENEILKAAKGSNFTKRCLLFVPGTAKNFVFVVWLIQNTVVFDSNSSERIYTTKRRKWTQDEPRGEWSERLLLFSEVREEDFYLNYTCRVYTSQGKSEGYFTLLPTDPNIILPIRLVFVGVTVLFVVSVAVYYAFRIDIVLCFRRAFPVLYTNKDLDGKLYDAYVAYPSPGAFGFSEEVERFALQTLPEVLEQACGYKLFIAGRDCLPGQAVVDSVDENLQASRRVLLLYTASTFIKKRHTSSTSSNNNNITKVGEEADQIQSGTSEGNGGVGFGGDDKAFSDTRQHLECVASMNRALLEGSLKVILVELEEITPAQLALFPESVRHLRKKQGAVCWWKVLQTRQQWRAWREDEERRGKDSQVSPSLSPRSRFWKEIRYHMPVRGKRAVYPEKTALLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2P4</accession>
    <name>A0A3B5M2P4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Poly [ADP-ribose] polymerase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M2P4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018605.1">
        <property type="protein sequence ID" value="ENSXCOP00000018373.1"/>
        <property type="gene ID" value="ENSXCOG00000013837.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155368"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01439">
        <property type="entry name" value="TCCD_inducible_PARP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051712">
        <property type="entry name" value="ARTD-AVP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45740:SF6">
        <property type="entry name" value="PROTEIN MONO-ADP-RIBOSYLTRANSFERASE PARP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="4">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="5">
        <location>
            <begin position="377"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="2">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="579" mass="65415" checksum="F14F04B051F72C69" modified="2018-12-05" version="1">MTDVSSGRRKKRKLAPKAPKGPSDSPKAIMLNSSLLLLEVPADTNTSLPVWDALRSLKVGVTWTVKPYGISINITPLTVKQEDITASCERENTSSVAETSSLQPQIQNTSCALLTFFHNSLPTLPHTYQPNQSLVNPISVSLPLIITHSPQGYQPGTMATIKLPPAVQAPTSVSGDLDVEPKKPNLSSFHTKVSHDILICDNFLLGTCDAGVKCKMHHTPYPFHWQLRSIKTHQWIDLSPRAQVLLERSYCCAEQSNVTLIDKQTCCVLDFDTMDLDDLSEYDAVRRLTNSESIEKNPHFPSRSKIYWCEGINYKEYSAVTFFAVYQNHIDTVDFQTVNGCPKQLSGIQSDFANCDPNAAAANFTIDPLQEFSSWYPPVWLQGSEEDYRLVDVPAGTQAYRSIKKFFHENLPETKIDIISLQQIQNLLHWDKYQRQKTHMQKHTKAEGPLERHLFHGTRKVASESICLHGFDPRVAGLNGHSHGFGSYFATNALMSHEYTEANESDEVGYMFLAKVLVGRVCLGKHYYRRPPNTKVSLYDACVDNKQSPRMFIVFDNCQCYPYYLIKYKKLSEEINIHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4B6</accession>
    <name>A0A3B5M4B6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kelch-like family member 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4B6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018215"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018446.1">
        <property type="protein sequence ID" value="ENSXCOP00000018215.1"/>
        <property type="gene ID" value="ENSXCOG00000013722.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155358"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd18444">
        <property type="entry name" value="BACK_KLHL1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18234">
        <property type="entry name" value="BTB_POZ_KLHL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000017">
        <property type="entry name" value="kelch-like protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000021">
        <property type="entry name" value="kelch-like protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF135">
        <property type="entry name" value="KELCH-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00501">
        <property type="entry name" value="KELCHREPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="528" mass="58219" checksum="A07E7B4F1FF94E52" modified="2018-12-05" version="1">MPFFSSLRLVLSSVSDYFAAMFTSDVREAKQDEVKMEGVDPEALWVLVQYAYTGRLELKEDTIESLLSASCLLQLSSVVQACCSFLVKQLHPSNCLGIRSYADAQGCHDLQRAAHAYTMEHFLEVVGAQEFLVLPVEEMERLLTSDDINVPDEETVVTSLLSWVSHDPATRQRHLPSLLAHVRLPLLQPQFLADLESNPLLRDSVECQRLLMEGMKYHLLPQRRPLLQSPRTRPRKATVGAMFAVGGMDASKGATSIEQYCLRRDTWRQVATMSGRRLQFGVAVLDGRLYVVGGRDGLKTLNTVECYNPHSKTWSVLPPMSTHRHGLGVAVLEGPMYAVGGHDGWSYLSTVERWDPQARQWSFVASMATPRSTVGVAVLNGKLYAVGGRDGSSCLRSVECFDPHTNRWTSCAPMAKRRGGVGVATWHGFLYAIGGHDAPASSLASRLSDCVERYDPQTDVWTTVAPMSISRDAVGVCLLGNRLYAVGGYDGQVYLNTVEAYDPQTNEWTQVASLCLGRAGACVVAVRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4H3</accession>
    <name>A0A3B5M4H3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Importin 7</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the importin beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4H3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014869"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015055.1">
        <property type="protein sequence ID" value="ENSXCOP00000014869.1"/>
        <property type="gene ID" value="ENSXCOG00000011269.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154666"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000053">
        <property type="entry name" value="Importin 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058669">
        <property type="entry name" value="TPR_IPO7/11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013713">
        <property type="entry name" value="XPO2_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF27">
        <property type="entry name" value="IMPORTIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08506">
        <property type="entry name" value="Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25758">
        <property type="entry name" value="TPR_IPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="9">
        <location>
            <begin position="22"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="868"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="871"/>
            <end position="897"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="988" mass="113187" checksum="07DF485A1D798AA6" modified="2018-12-05" version="1">MDPESLVEALRGTMDPNLREAAERQLNEAHTQVNFMSTLLRVTMSDQLDLPVRQAGVIYLKNKIIQHWSDGEVAGTEPPVNNIPEEDRQFIRDNIVEAIIQSPERIRVQLTTCIHHIIKHDYPGKWTSIVDKIGFYLQSDNSLAWLGILLCLYQLVKNYEYKKPEERQPLVAAMHIFMPMLKDRFIQLLQDHSNESVLIQKQIFKILYALFQYNLPLELINRQILTEWMEILKTVVDRDVPPETMQIDEDERPELPWWKCKKWALHILARLFERYGSPGNTTKEYAEFAELFLKEYAAAAQQVLLKVLYQYKEKQYVAPRVLQQTLNYINQGIAHALTWKNLKPHIQGIIQDVVFPLMCYTDSDEELWQEDPYEYIRMKFDVFEDFISPTTAAQTLLFTACNKRKEVLQKTMGFCYQILTDPNSDPRKKDGALHMIGSLAEILLKVTPDYHFLLIKPQCGWTSSSSYHIILISVKFKSDQNLQTALELTRNCLINDNEMPVKVEAAIALQVLISNQEKAKEYIAPYIRPVMQALLHIVRETENDDLTNVIQKMICEYSEEVTPIAVEMTQHLAMTFNQVIQTGPDEEGGDDKAVTAMGILNTIDTLLSVVEDHKEITQQLEGICLQVIGTVLQQHVLEFYEEILSLAHSLTCQQVSPQMWQLLPLVYEVFQQDGFDYFTDMMPLLHNYVTVDTDTLLSDTKYLEIIYSMCKKVLTGDPGEDPECHAAKMLEVIILQCKGRGIDQVVPLFVGTALERLTREVKTSELRTMCLQVAIAALYYSPPLLLNTLENLRFPNSTEPITNHFITQWLKDVDCFLGLHDRKMCILGLCALIDLDHRPQAINQVGGQLLPAAILLFSGLKRAYACRAEHENEDDDEDEDGEEEDENAELGSDEDDIDEEGQEYLEMLAKHAGEDGDDEDWEEDDAEETALEGYTTSVDDEDNLVDEYQIFKAILQKSKMIEKHGGYKFTAPVVPSTFNFGGTAPGMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6A9</accession>
    <name>A0A3B5M6A9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zinc finger CCCH domain-containing protein 15</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ZC3H15/TMA46 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6A9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018945"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019182.1">
        <property type="protein sequence ID" value="ENSXCOP00000018945.1"/>
        <property type="gene ID" value="ENSXCOG00000014248.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015818"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000050">
        <property type="entry name" value="AGAP008634-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032378">
        <property type="entry name" value="ZC3H15/TMA46_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681:SF0">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681">
        <property type="entry name" value="ZINC FINGER-CONTAINING PROTEIN P48ZNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16543">
        <property type="entry name" value="DFRP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="15">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="15">
        <location>
            <begin position="172"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="12">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="12">
        <location>
            <begin position="172"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="296"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="216"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="300"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="348"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="408" mass="46826" checksum="72A829FA609175A2" modified="2018-12-05" version="1">MPPKKPAPATGNKKTQEKKKEKIIEDKTFGLKNKKGAKQQKYIKNVTQQVKYGQQSSRQIAEADKTTKKADKKKELDELNELFKPVVAAQKVSKGVDPKSVLCAFFKQGQCTKGDKCKFSHDLSMERKCEKRSVYVDERDEDLEKDTMENWDEKKLEEVVNKKHGEAEKKKAKTQIVCKYFLEAIENNKYGWFWVCPAGGDNCMYRHALPPGFVLKKDKKKEEKEEEISLEELIESERAALGVNVTRITLETFLAWKKRKRQEKVDKAREELEKKKADFKAGKSLIVSGREVFEFRPELVDDDDDEADDTQYADETKEEDEEETDLTDVQDIDVSRFVPQEVDHSGITVASTDRFSSRNEVATGDNGEEEDKPDGEDEEEDVPVDENLFTGEDLEELDEELNTLGLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6G1</accession>
    <name>A0A3B5M6G1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SPRY domain containing 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027868216.1">
        <property type="nucleotide sequence ID" value="XM_028012415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6G1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016981"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017200.1">
        <property type="protein sequence ID" value="ENSXCOP00000016981.1"/>
        <property type="gene ID" value="ENSXCOG00000012806.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114141697"/>
    <dbReference type="KEGG" id="xco:114141697"/>
    <dbReference type="CTD" id="283377"/>
    <dbReference type="GeneTree" id="ENSGT00940000159780"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050617">
        <property type="entry name" value="E3_ligase_FN3/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099:SF16">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MIDLINE-1-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="1">
        <location>
            <begin position="28"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="224" mass="25236" checksum="2F61D72008D2ECFB" modified="2018-12-05" version="1">MAIPIRISPLCRWTGRTLSSLSAGHRREVSLSAKRRFICTSTGNHLQFRLNERTAHSSLDLFKKDTGIIYRMLGLDPSQVQDDPERFREWAVVLGDKKISSERHYWEVTVKKSQEFRLGVAEAVMSRDECVGTSSSSWVFGYAQRKWFAMTGSKVVPVTLVGKPDRVGILLDYDAGFVGLVDIEKPRIIHTLRARFTAPVCPAFGLWDGEILTHSGLEEPEGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7U6</accession>
    <name>A0A3B5M7U6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7U6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020348"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020596.1">
        <property type="protein sequence ID" value="ENSXCOP00000020348.1"/>
        <property type="gene ID" value="ENSXCOG00000015251.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001057"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019375">
        <property type="entry name" value="Ribosomal_bS1m"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13447">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13447:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10246">
        <property type="entry name" value="MRP-S35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="186" mass="20279" checksum="343D93B70D0278A5" modified="2018-12-05" version="1">MAALWRLRFGCRSAFRFSSSVSVRACFSTDSEDGTRAGAGFAAAFQRQAELQREAETSAPDQSASISGQEQTFASLFQAVPVRDKVVVGNIFHVVRDDLYIDFGGKFHCVCRRPAAGGERLQRGSRVRLRVLDPELTARFLGAKTDTTLLEAQAVLLGLHAESIRLQVSVCNNRHKKQEASSGSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7W3</accession>
    <name>A0A3B5M7W3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7W3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019597"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019842.1">
        <property type="protein sequence ID" value="ENSXCOP00000019597.1"/>
        <property type="gene ID" value="ENSXCOG00000014723.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000267077"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008375">
        <property type="term" value="F:acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006759">
        <property type="entry name" value="Glyco_transf_54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062:SF1">
        <property type="entry name" value="ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 4-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYLTRANSFERASE VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="78" mass="8868" checksum="91840FA5F2BED9B8" modified="2018-12-05" version="1">MGVPSVKREVHSYLTDTLSSLMSELSPAEKDDCVVVVLIAEVLTAKLPKYGKNYNKTYRTRERQTFNSTQKHVIPKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAC8</accession>
    <name>A0A3B5MAC8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAC8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020515"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020765.1">
        <property type="protein sequence ID" value="ENSXCOP00000020515.1"/>
        <property type="gene ID" value="ENSXCOG00000015368.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017045"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021850">
        <property type="entry name" value="Symplekin/Pta1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032460">
        <property type="entry name" value="Symplekin/Pta1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022075">
        <property type="entry name" value="Symplekin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15245:SF20">
        <property type="entry name" value="SYMPLEKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15245">
        <property type="entry name" value="SYMPLEKIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11935">
        <property type="entry name" value="SYMPK_PTA1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12295">
        <property type="entry name" value="Symplekin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Symplekin/Pta1 N-terminal" evidence="5">
        <location>
            <begin position="87"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Symplekin C-terminal" evidence="6">
        <location>
            <begin position="844"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="422"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1063" mass="119401" checksum="63DB5E5C4F8BC6E7" modified="2018-12-05" version="1">VVELLNQAALIPADEKLTVLKQVQELIINKDPSLLDNFLDEIIAFQTDRSMEVRKFVIGFIEEAKRDNELLLRLIANLNLLLKDDSVNVVKKAILSLTQLYKVALQWLVRSRSVSEMQEACWDLVSQMTGDVLSMLDSENDGVRTHAIKFTESLIVTLSPRTPESDVPKRQEGDISLDKVPRDHPYIRYALCDEGKTALDKLLKFMVHPAISSINLTTALGSLATIARQRPMFMSEVVQAYETLHNLPPTLAKSQVSSVRKNLKLHLVSVLKHPCSLEFQVQIGTLLLDLGMPQSEISRNTPLPREQRKRPRYEPAAEGKKVKMLIEDDEDKEEPVPLAAPMPAAAPTPQSAIDLTAEFLRPLLTSENVANLQVLISMVYLPDVMPASFQATYTPVESAGTDAQIKHLARLMATQMTAAGIGPLEQCKAREDESGKEEPAEDESGGKDVLIRRSLSSMVGQAISVVGGYSEKPPAPEAPAIKRLPEPILPSVQTMAGAGGRKKVFRLADVVQPLSDSQIGSLSSRAVKRILHSEKAVTQSGMSHVRVKLLSRLVTQFEGVMKEDVLDYVLEDMRCRSDLAFSLLYQEYNSYLSQLPAGLLDGYDHCLYTLLSGLQEKPEQRDLFTKLVLEAPIITESALDVIRRYCEDEFQSRVYLGMTTLKELIVKRPSRQFQYLHVLLDLSSHEKEKTALAFLKRMYEKDHLRDYIEKFALNYLQLLVHPNPPSLLFGADKDTVAAPWTEETVRQCLFLYLSLLPLNHRLVHELASVYTEAIADIKRSVLRAIEQPIRGMGMNSPELLLLVENCPKGAETLVTRCLHILTDKPPSPELVERVRDLYHKRVPDVRFLIPVINGLEKNEVIQALPKLIKLNPIVVKEVFNRLLGTQHEGSSSVSPLTPGDLLIALHNIDSTKCDMKSIIKTNLCFGEKNVYTSEVLAVVMQQLMEQNPLPMLLMRTVIQSLTMYPRLGGFVMNILSRLIVKQVWKYPKVWEGFVKCCQRTKPQSYSVLLQLPPLQLASVFERCPEMRAPLLQHVLSFTPHQPQQAHIPNSVMAVLEAHKKLDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAD7</accession>
    <name>A0A3B5MAD7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAD7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020792.1">
        <property type="protein sequence ID" value="ENSXCOP00000020542.1"/>
        <property type="gene ID" value="ENSXCOG00000015391.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000245018"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="104" mass="10882" checksum="085518BDFE27E151" modified="2018-12-05" version="1">MLLPGPVFSEGFTRKPTMTGSLSSEASTCSKENDQSGSSCSAEASWVEDASTEAAVLAGCAASAGGLLGSCSLRPTSSILRRRLVTWATARYKDSSSGWPWKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAL5</accession>
    <name>A0A3B5MAL5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endomembrane system</location>
            <topology evidence="9">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAL5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021293"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021553.1">
        <property type="protein sequence ID" value="ENSXCOP00000021293.1"/>
        <property type="gene ID" value="ENSXCOG00000015933.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159564"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003378">
        <property type="entry name" value="Fringe-like_glycosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10811">
        <property type="entry name" value="FRINGE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02434">
        <property type="entry name" value="Fringe"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fringe-like glycosyltransferase" evidence="10">
        <location>
            <begin position="8"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Fringe-like glycosyltransferase" evidence="10">
        <location>
            <begin position="96"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="211" mass="23876" checksum="33E4BEC2F6BA36A4" modified="2018-12-05" version="1">LKECVYKEDEELSSKGYNMVVTGCQSDHSQQALSCKMSVEYDGFMASNKKWFCHVDDDNYVNPEGLLSLLSTFPQEGDIYVGKPSLDKPITAHELRRLAEKMSPWASGPHFERTSARIRLPDDCTVGFIVEKMLGVAMVHCPLFHSHLENLLLISQRSLPQQVTLSYGMFENKMNSIEVKGSFSKEEDPSRLVSPSIHLQMRKICEQCPTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAU6</accession>
    <name>A0A3B5MAU6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Leptin receptor overlapping transcript-like 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Negatively regulates growth hormone (GH) receptor cell surface expression in liver. May play a role in liver resistance to GH during periods of reduced nutrient availability.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OB-RGRP/VPS55 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027864957.1">
        <property type="nucleotide sequence ID" value="XM_028009156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAU6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020707"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020959.1">
        <property type="protein sequence ID" value="ENSXCOP00000020707.1"/>
        <property type="gene ID" value="ENSXCOG00000015526.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114139299"/>
    <dbReference type="GeneTree" id="ENSGT00390000006503"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060400">
        <property type="term" value="P:negative regulation of growth hormone receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007262">
        <property type="entry name" value="Vps55/LEPROT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12050:SF4">
        <property type="entry name" value="LEPTIN RECEPTOR OVERLAPPING TRANSCRIPT-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12050">
        <property type="entry name" value="LEPTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04133">
        <property type="entry name" value="Vps55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="131" mass="14305" checksum="82FB881A4058488F" modified="2018-12-05" version="1">MAGIKALISLSFGGAIGLMFLMLGCALPVYDKYWPLFLLFFYILSPIPYCISRRVVDDTDSASNACKELAIFLTTGIVISAFGLPIVFARAEVIAWGACALVLTGNVVIFGTILGFFLVFGSNDDFSWQQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBK6</accession>
    <name>A0A3B5MBK6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Myristoylated alanine-rich C-kinase substrate</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MARCKS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027856187.1">
        <property type="nucleotide sequence ID" value="XM_028000386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBK6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018651"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018886.1">
        <property type="protein sequence ID" value="ENSXCOP00000018651.1"/>
        <property type="gene ID" value="ENSXCOG00000014042.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114134092"/>
    <dbReference type="KEGG" id="xco:114134092"/>
    <dbReference type="CTD" id="323201"/>
    <dbReference type="GeneTree" id="ENSGT00730000111419"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060027">
        <property type="term" value="P:convergent extension involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003381">
        <property type="term" value="P:epithelial cell morphogenesis involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900144">
        <property type="term" value="P:positive regulation of BMP secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060541">
        <property type="term" value="P:respiratory system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010842">
        <property type="term" value="P:retina layer formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035914">
        <property type="term" value="P:skeletal muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002101">
        <property type="entry name" value="MARCKS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14353:SF9">
        <property type="entry name" value="MYRISTOYLATED ALANINE-RICH C-KINASE SUBSTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14353">
        <property type="entry name" value="MYRISTOYLATED ALANINE-RICH C-KINASE SUBSTRATE MARCKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02063">
        <property type="entry name" value="MARCKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00963">
        <property type="entry name" value="MARCKS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="109"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="205"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="219" mass="22410" checksum="A16A4C17BCFBBB0A" modified="2018-12-05" version="1">MGAQISKTAGKDEAAVEKPAEGAAVASKANGQENGHAKTNGDASPAAEDANKADVQANGSTPTEEVPKEEGEKVEDAEAQGDKEASTTNGETSAKPEEGTPSTSEDGKQKKKRFSFKKPSFKLSGFSFKKTKKESEEATEEGAAAAAEPAEGEKSTEAPAEETKPAEGSGGEIKETAADEVKAEELKAEQAAEGEEKPAKVSPTEPEMAASPEAAAAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBZ1</accession>
    <name>A0A3B5MBZ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Complex 1 LYR protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBZ1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021055"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021314.1">
        <property type="protein sequence ID" value="ENSXCOP00000021055.1"/>
        <property type="gene ID" value="ENSXCOG00000015758.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001810"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090324">
        <property type="term" value="P:negative regulation of oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20265">
        <property type="entry name" value="Complex1_LYR_ETFRF1_LYRM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045296">
        <property type="entry name" value="Complex1_LYR_ETFRF1_LYRM5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052000">
        <property type="entry name" value="ETFRF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21024:SF0">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN REGULATORY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21024">
        <property type="entry name" value="GROWTH HORMONE-INDUCIBLE SOLUBLE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="2">
        <location>
            <begin position="44"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="119" mass="14265" checksum="6B621ED83DBC5F6F" modified="2018-12-05" version="1">MCRNAKILTYFLVHFSCSSTEVSDIFTLFCFAALWMMANPLRPEVVRLYKNLLYLGREYPKGSNYFKDRLRAAFIKNKSVQDPEQIKAMIARGEYVARELEALYYLRKYRAMKKRYYEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCX3</accession>
    <name>A0A3B5MCX3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Hsp90 co-chaperone Cdc37</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Hsp90 chaperone protein kinase-targeting subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Forms a complex with Hsp90.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CDC37 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCX3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021467"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021729.1">
        <property type="protein sequence ID" value="ENSXCOP00000021467.1"/>
        <property type="gene ID" value="ENSXCOG00000016067.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013443"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.610">
        <property type="entry name" value="Cdc37, Hsp90 binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004918">
        <property type="entry name" value="Cdc37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013874">
        <property type="entry name" value="Cdc37_Hsp90-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038189">
        <property type="entry name" value="Cdc37_Hsp90-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013855">
        <property type="entry name" value="Cdc37_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800">
        <property type="entry name" value="CDC37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800:SF3">
        <property type="entry name" value="HSP90 CO-CHAPERONE CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01070">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01071">
        <property type="entry name" value="CDC37_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101391">
        <property type="entry name" value="Hsp90 co-chaperone CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cdc37 N-terminal" evidence="8">
        <location>
            <begin position="2"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc37 Hsp90 binding" evidence="7">
        <location>
            <begin position="115"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="192" mass="22168" checksum="73C6E18D214CF170" modified="2018-12-05" version="1">MRIDCGVWDHIYVSHDDDVTSPFVDTPSSFRMRHRVSRDSCCSSCELCGMLETASGSPATTRTPGRADEEEDEEPEAELKKVQAEVKKLEKDEKFFLGHLEAHRRGEKKLEKVLARKGRGKVHIVCKTQQKHKTFVEKYAKEMKHFGMLRRWDDSQKYLSDNPHLVCEESANDLVVLCIDFEIDEVRAVERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MET6</accession>
    <name>A0A3B5MET6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RabBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MET6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022080"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022349.1">
        <property type="protein sequence ID" value="ENSXCOP00000022080.1"/>
        <property type="gene ID" value="ENSXCOG00000016501.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159060"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15764">
        <property type="entry name" value="FYVE_Slp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041282">
        <property type="entry name" value="FYVE_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044134">
        <property type="entry name" value="FYVE_Slp4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051745">
        <property type="entry name" value="Intracell_Transport_Effector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010911">
        <property type="entry name" value="Rab_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14555">
        <property type="entry name" value="MYELIN-ASSOCIATED OLIGODENDROCYTIC BASIC PROTEIN MOBP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14555:SF6">
        <property type="entry name" value="RAB EFFECTOR MYRIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02318">
        <property type="entry name" value="FYVE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50916">
        <property type="entry name" value="RABBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RabBD" evidence="3">
        <location>
            <begin position="7"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="136" mass="15515" checksum="7F00C63937296723" modified="2018-12-05" version="1">MPEAPDLINLGFLSDSERELILEVLRRDEELRMVEDKRVRKLKSELLEIKRKGAKRGGGSYSERSCGRCQEPLSRLPVFSNQCKICSHYVCRNCQSVLPNGSWLCNVCVKETRRKTLENYSSSQTRASAQTEKSAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGX7</accession>
    <name>A0A3B5MGX7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">G-protein coupled receptors family 2 profile 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGX7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023002"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023279.1">
        <property type="protein sequence ID" value="ENSXCOP00000023002.1"/>
        <property type="gene ID" value="ENSXCOG00000017189.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157432"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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            <begin position="6"/>
            <end position="30"/>
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            <begin position="195"/>
            <end position="217"/>
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            <begin position="7"/>
            <end position="248"/>
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            <dbReference type="PROSITE" id="PS50261"/>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHB7</accession>
    <name>A0A3B5MHB7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Coronin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1 7">Belongs to the WD repeat coronin family.</text>
    </comment>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:actin filament binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
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    <keyword evidence="3 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="75"/>
            <end position="117"/>
        </location>
    </feature>
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            <end position="167"/>
        </location>
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            <end position="434"/>
        </location>
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    </feature>
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    </evidence>
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            <dbReference type="RuleBase" id="RU280818"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="472" mass="52972" checksum="2C776D39484FE17B" modified="2018-12-05" version="1">MKKIYLTCFFIIIIIIIIILVLCLECFLFLAHVTHLVFLCGKNPSLYVNASSCSWDLGLCCNCPSGRIDKSYPTVCGHTGPVLDIDWCPHNDQVIASGSEDCTVMVWQIPENGLVSSMSEPVVVLEGHSKRVGIITWHPTARNVLLSAGCDNQIIIWNVGTGEAMISLEDMHPDVIYNVTWNRNGSLICTSCKDKSIRVIDPRKETIIAEKEKAHEGARPMRAIFLSNGNILTTGFSRMSERQLALWNTENMEEPMTVNEMDTSNGVLLPFYDPDTSVVYLCGKGDSSIRYFEITDEAPYVHFLNTFASKEPQRGMGYMPKRGLDVNKCEIARFYKLHERKCEPIVMTVPRKSDLFQDDLYPDTAGPDPALEAEDWFEGKNGDPILISLKNGYVPGKNREFKVVKKNILDSKVNKNMEKSSPANKSASSTPSIKSEAKMEEILKEIKSLKDLISSQEKRIIKLEEQMSKVAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHN9</accession>
    <name>A0A3B5MHN9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSXCOT00000020887.1">
        <property type="protein sequence ID" value="ENSXCOP00000020636.1"/>
        <property type="gene ID" value="ENSXCOG00000015460.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000003">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase kappa isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF449">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="3">
        <location>
            <begin position="13"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="186"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="277" mass="31850" checksum="C4F15142916290FE" modified="2018-12-05" version="1">MLRVDSQSNSSPLREEFQTLNSVTPHLDVEECSIALLPRNREKNRSMDVLPPDRALAFLVTTEGESNNYINAALADSFHRQAAFIVTPHPLPGTTADFWRLVFDYGCTAVVMLNQLNQSNSYWPEPGLQQYGPMEVEFLSMSADEDVITRLFRVKNVTRLQEGQLVVCQFQFLRWSAYRDVPDSKKAFLNLLAQVHKWQRECGEGRTVVHCLNGGGRSGTFCACNILLEMIQYQNMVDVFYSVKTLRNCKPNMVESLDQYRFCYDLVLEYLDCFEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MII0</accession>
    <name>A0A3B5MII0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Myogenesis regulating glycosidase</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MII0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023557"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023838.1">
        <property type="protein sequence ID" value="ENSXCOP00000023557.1"/>
        <property type="gene ID" value="ENSXCOG00000017594.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161008"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048741">
        <property type="term" value="P:skeletal muscle fiber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06592">
        <property type="entry name" value="GH31_NET37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000037">
        <property type="entry name" value="Putative family 31 glucosidase KIAA1161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050985">
        <property type="entry name" value="Alpha-glycosidase_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43053">
        <property type="entry name" value="GLYCOSIDASE FAMILY 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43053:SF4">
        <property type="entry name" value="MYOGENESIS-REGULATING GLYCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 31 TIM barrel" evidence="6">
        <location>
            <begin position="307"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 31 C-terminal" evidence="7">
        <location>
            <begin position="619"/>
            <end position="699"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF21365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="703" mass="81319" checksum="31A4B594C8BDB9DA" modified="2018-12-05" version="1">FPENPLGKTNSDIQPEAPCSTFLMICTTSPQGFDVIPKQKHTKDSRPLVGAGVIGLFLVIAAVTAWCYYIASLRKAELLKTQLLDLNKDGYIIRNQGGSIVFRMDFRSGTLDLDSCSKEEEILRCDRTTDRKLKFFIKTVRPKDTVQCYRVRWEELVPNIPVEHAMTYKFAHWYGGAVSAIQHWPISISGQQAPKPFVTSDIYSNREEFGGILESYWLSSNATAIKINNSVPFHLGWNDTEKTMYFQARYHDTPFKPNPGEAPCAELSYRVCVGLDVTSIHKYMVRRYFNKPYKVPAKVMFEHPIWSTWALYKRDIDQEKLLTYAANIRKYNFNCSHLELDDRYTKRYGDFEFDPVKFPNASEMFQKLKSDGFLVSLWIHPFVNYDSENFHPLVERGLFVREPTGRLPALVHWWNGIGGILDFTNPEAREWFASQLRSLRSKYGVSSFKFDAGETNYLPWKFSTKNHIHDPSFFTRRYTEMAVHYNDRAELRSGYQSQNISCFFRPTDRDSVWGYELGLKSLIPTVLTISILGYQFILPDMIGGNAYLNHTSGDRALPDRELYIRWLELSAFMPSMQFSIPPWEYDNEVVEIARKYTAIHKSLVAPRVLELAGEVLDTGDPIIRPLWWIATGDETAYKIDSQFLIGDDLMVAPVLEPGKQERDIYLPAGHWRSYKGERFDIKKPLHLTDYPVDLDEIAFFLWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIR5</accession>
    <name>A0A3B5MIR5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein S100</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">S100 calcium-binding protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the S-100 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027880928.1">
        <property type="nucleotide sequence ID" value="XM_028025127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIR5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023647"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023930.1">
        <property type="protein sequence ID" value="ENSXCOP00000023647.1"/>
        <property type="gene ID" value="ENSXCOG00000017666.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114149339"/>
    <dbReference type="KEGG" id="xco:114149339"/>
    <dbReference type="CTD" id="170591"/>
    <dbReference type="GeneTree" id="ENSGT00940000161125"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000044">
        <property type="entry name" value="Protein S100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001751">
        <property type="entry name" value="S100/CaBP7/8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013787">
        <property type="entry name" value="S100_Ca-bd_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639:SF134">
        <property type="entry name" value="PROTEIN S100-A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639">
        <property type="entry name" value="S100 CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01023">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01394">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00303">
        <property type="entry name" value="S100_CABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="50"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="98" mass="11029" checksum="3BCE3F1E4C839D62" modified="2018-12-05" version="1">MPSQLEGAMGALITVFYNYSGNDGDKHKLNKGELKELLHSELSDFLTSQKDPMLVEKIMNDLDTNKDNEVDFNEFVVLVAALTVACNDFFQEQNKNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKN6</accession>
    <name>A0A3B5MKN6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKN6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020084"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020331.1">
        <property type="protein sequence ID" value="ENSXCOP00000020084.1"/>
        <property type="gene ID" value="ENSXCOG00000015058.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01010000224828"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="140" mass="15484" checksum="91B238B5AEEB19DF" modified="2018-12-05" version="1">MCNVSHFLTNTANGLDNLPVPGISVLKQNQPETTAPRQETTAPRQETTETTAPRQETTAPRQETTAPRQETTAPRQETTAPRQETTAPRQETTAPRQETTAPRQETTAPRQETTAPRQETMAPRQETTHCPPLICPDAKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MM92</accession>
    <name>A0A3B5MM92_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SOWAHA-C winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the SOWAH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MM92"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020589"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020839.1">
        <property type="protein sequence ID" value="ENSXCOP00000020589.1"/>
        <property type="gene ID" value="ENSXCOG00000015424.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183003"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058889">
        <property type="entry name" value="WHD_SOWAHA-C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14491:SF9">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN SOWAHB-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14491">
        <property type="entry name" value="SOSONDOWAH, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25877">
        <property type="entry name" value="WHD_SOWAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SOWAHA-C winged helix-turn-helix" evidence="6">
        <location>
            <begin position="3"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="215"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="327"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="353" mass="40097" checksum="36382FE119F8DE51" modified="2018-12-05" version="1">MGDVTEESLLNYFFSAGGNSGRVKNADMLKTFKPFIGHSDPQLRAKYREEFKLIIDRIAVVKSENGEKYLVLKKKYRNLLQERDTRHHETDGNAQRNQSLARVTAAAQWEAMDILTSSPSKKEEQDEPMSCGEPDEEVSCRFLFKLKCSKIKLMRSLNNGEDELDRDSTSKVKSKWCFNPTDLDPTEKEWIYHAASARVPDLSQLLMQEPSLANKKVTALHWAAKHGNENMAVLVADAGADVNTKSGYTPLHIAALHGHRHILDLLIGTYGAKENVRDYSGRLPGHYLNIKDPEGPQEDFAQARERNKNRKLVSLFHSKKKWGSAEELAPIEEERTASHQLALPPFRPRKFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MMB5</accession>
    <name>A0A3B5MMB5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="3">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 is involved in the regulation of cell adhesion and cell migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. Involved in somitogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MMB5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020614"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020864.1">
        <property type="protein sequence ID" value="ENSXCOP00000020614.1"/>
        <property type="gene ID" value="ENSXCOG00000014212.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155503"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005005">
        <property type="term" value="F:transmembrane-ephrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10472">
        <property type="entry name" value="EphR_LBD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05065">
        <property type="entry name" value="PTKc_EphR_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09552">
        <property type="entry name" value="SAM_EPH-B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00185">
        <property type="entry name" value="TNFRSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000041">
        <property type="entry name" value="ephrin type-A receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1770:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000015">
        <property type="entry name" value="Ephrin type-B receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000004">
        <property type="entry name" value="Ephrin type-B receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000110">
        <property type="entry name" value="Ephrin type-B receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1770">
        <property type="entry name" value="ephrin a2 ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027936">
        <property type="entry name" value="Eph_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001090">
        <property type="entry name" value="Ephrin_rcpt_lig-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050449">
        <property type="entry name" value="Ephrin_rcpt_TKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011641">
        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016257">
        <property type="entry name" value="Tyr_kinase_ephrin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001426">
        <property type="entry name" value="Tyr_kinase_rcpt_V_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877">
        <property type="entry name" value="EPH RECEPTOR A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877:SF11">
        <property type="entry name" value="EPHRIN TYPE-B RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14575">
        <property type="entry name" value="EphA2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25599">
        <property type="entry name" value="Ephrin_CRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01404">
        <property type="entry name" value="Ephrin_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07699">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000666">
        <property type="entry name" value="TyrPK_ephrin_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00615">
        <property type="entry name" value="EPH_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51550">
        <property type="entry name" value="EPH_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00790">
        <property type="entry name" value="RECEPTOR_TYR_KIN_V_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 22 24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="14 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9 22 24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 26" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="15" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017202814" description="receptor protein-tyrosine kinase" evidence="26">
        <location>
            <begin position="16"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="532"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="Eph LBD" evidence="30">
        <location>
            <begin position="4"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="29">
        <location>
            <begin position="313"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="29">
        <location>
            <begin position="424"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="610"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="28">
        <location>
            <begin position="902"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="21">
        <location>
            <position position="735"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <begin position="616"/>
            <end position="624"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22 24">
        <location>
            <position position="642"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="51"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00055965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR000666-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS51550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="975" mass="109208" checksum="0B6A8668017133C6" modified="2018-12-05" version="1">TLLFFMLMDSTTATAELGWTVYPVSGSSDNSWEEVSGYDEHMNTIRTYQVCNVFDSNQNNWVRTKYIRRRGAQRIHVEMKFSVRDCSSIPNVPGSCKETFNLYYYESDSDTATRTSPAWMENPWIKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSKNGFYLAFQDYGGCMSLIAVRVFYRKCPRIITNGALFQETLSGAESTSLVAARGVCIPNAEEVDVPIKLYCNGDGEWMVPIGRCMCKAGNEAVENGTVCRACPSGFFKPTQGDESCMQCPINSRTTSEGAINCICRNGYYRTDSDPLQMPCTTVPSAPQTVISSVNETSVMLEWMPPRDSGGREDVVYNIICKSCGGGRGGCTRCGDNVQFLPRQLGLTKSRVYISDLLAHTQYTFEVQAVNGVSDQSPYSPQYASVNITTNQAAPSTVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKDQSEYNSTTIRSQTNTAVIRGLKSGSIYVFQVRARTVAGFGRYSGKLYFQTMTEEEYNTSIQEKLPLIIGSAAAGLVFLIALVVIIIVCNRRGFDRGDSEYTDKLQHYTSGHMSPGMKIYIDPFTYEDPNEAVREFAKEIDISCVKIEQVIGAGEFGEVCSGNLKLPGKREMFVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSSPVMIITEFMENGSLDTFLRQNDGQFTVIQLVGMLRGIASGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDTSDPTYTSALGGKIPIRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDMTNQDVINAIEQDYRLPPPMDCPNALHQLMLDCWQKDRNNRPKFSQIVNNLDKMIRNPNTLKAMTPLSSGVHLPLLDRSIPDFSTFSTVDEWLDAIKMGQYKENFANSDFSTFDVVSQMTMDDILRIGVTLAGHQKKILNSVQMMRAQMNQIQSVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MMU7</accession>
    <name>A0A3B5MMU7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Adenosine deaminase</fullName>
            <ecNumber evidence="5">3.5.4.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Extracellular side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.4" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MMU7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020789"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021042.1">
        <property type="protein sequence ID" value="ENSXCOP00000020789.1"/>
        <property type="gene ID" value="ENSXCOG00000015570.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183113"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060205">
        <property type="term" value="C:cytoplasmic vesicle lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006154">
        <property type="term" value="P:adenosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043103">
        <property type="term" value="P:hypoxanthine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046103">
        <property type="term" value="P:inosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060169">
        <property type="term" value="P:negative regulation of adenosine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009168">
        <property type="term" value="P:purine ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042110">
        <property type="term" value="P:T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006650">
        <property type="entry name" value="A/AMP_deam_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001365">
        <property type="entry name" value="A_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006330">
        <property type="entry name" value="Ado/ade_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01430">
        <property type="entry name" value="aden_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11409:SF43">
        <property type="entry name" value="ADENOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00962">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00485">
        <property type="entry name" value="A_DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Adenosine deaminase" evidence="10">
        <location>
            <begin position="22"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="349" mass="39879" checksum="9126E184722A7428" modified="2018-12-05" version="1">MCFTYKGRKSAKMLTRQILELIELHVHLDGAIRVQTILDVAKRRGISLPATTAEELKEKIIIHEPATLTKFLEKFEEYMHVIAGDKDAIKRIAYEFVEDKAKEGVIYVEVRYSPHFLADCDVGKPIPWNQTEGDLSPDEVVRLVNEGLREGERAFNIKARSILCCMRHMPSWSIEVVELCKKYRNDGVVAIDLAGDESMNLEANPEHRTAYEEAVRCGIHRTVHAGEAVEVLKAERVGHGYNTLQDPALYRKLLEQNMHFEMCPVSSKLTGACDSDFTQHPIITFRKDKANYSLNTDDPLIFNSNLLLDYSVVHQHMGFTEEEFKRLTKTQDGRSVLVERKEKNDKSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNI5</accession>
    <name>A0A3B5MNI5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cocaine- and amphetamine-regulated transcript protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CART family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNI5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021039"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021297.1">
        <property type="protein sequence ID" value="ENSXCOP00000021039.1"/>
        <property type="gene ID" value="ENSXCOG00000015740.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018319"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008343">
        <property type="term" value="P:adult feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032099">
        <property type="term" value="P:negative regulation of appetite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22741">
        <property type="entry name" value="CART_CTD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.40.30:FF:000001">
        <property type="entry name" value="Cocaine-and amphetamine-regulated transcript protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.40.30">
        <property type="entry name" value="CART, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009106">
        <property type="entry name" value="CART"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036722">
        <property type="entry name" value="CART_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16655">
        <property type="entry name" value="COCAINE AND AMPHETAMINE REGULATED TRANSCRIPT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16655:SF5">
        <property type="entry name" value="COCAINE- AND AMPHETAMINE-REGULATED TRANSCRIPT 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06373">
        <property type="entry name" value="CART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64546">
        <property type="entry name" value="Satiety factor CART (cocaine and amphetamine regulated transcript)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0527">Neuropeptide</keyword>
    <keyword evidence="6" id="KW-0529">Neurotransmitter</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017331551" description="Cocaine- and amphetamine-regulated transcript protein" evidence="9">
        <location>
            <begin position="29"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="107" mass="11969" checksum="5965A42FB8CDD84F" modified="2018-12-05" version="1">CCFVGMWARAVLCAALLSVLCPAGSTEAERDEREVQDYSNSNRLLGALHEVLEKLQTKRINPWEKKYGQVPSCDLGEHCAIRKGSRIGKMCDCPRGAFCNFFLLKCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNP0</accession>
    <name>A0A3B5MNP0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNP0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021099"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021358.1">
        <property type="protein sequence ID" value="ENSXCOP00000021099.1"/>
        <property type="gene ID" value="ENSXCOG00000015786.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00580000081898"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF523">
        <property type="entry name" value="STRESS-INDUCED-PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="114" mass="13078" checksum="237FF4F41CAA81AF" modified="2018-12-05" version="1">MEKVRQSNEACQRGDFQEAVCLYGDALQADPQNCILYSNRSAAFLKLGQHQAALEDAEKACGLNPKWPKVRIPAFLFPRKIYEYSCTFEMGRSHIGDDIVTQMFHFLSNFHFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MP38</accession>
    <name>A0A3B5MP38_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tudor domain containing 15</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000025548"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025856.1">
        <property type="protein sequence ID" value="ENSXCOP00000025548.1"/>
        <property type="gene ID" value="ENSXCOG00000019086.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162581"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000018">
        <property type="entry name" value="Serine/threonine-protein kinase 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050621">
        <property type="entry name" value="Tudor_domain_containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948">
        <property type="entry name" value="TUDOR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948:SF7">
        <property type="entry name" value="TUDOR DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tudor" evidence="2">
        <location>
            <begin position="65"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="2">
        <location>
            <begin position="286"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="2">
        <location>
            <begin position="505"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="2">
        <location>
            <begin position="743"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="2">
        <location>
            <begin position="958"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="2">
        <location>
            <begin position="1256"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="2">
        <location>
            <begin position="1526"/>
            <end position="1583"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="2">
        <location>
            <begin position="1739"/>
            <end position="1796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1144"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1374"/>
            <end position="1412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1174"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1374"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1403"/>
            <end position="1412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1919" mass="216403" checksum="778C94EC7DCDF716" modified="2018-12-05" version="1">MKKSLSNTPQPLIYTDPAEYHNLTHLDWSPEATLIHFQGQYPSICELDYNILQQELQNVPKTKAAVDVADFCLVEDVTSARWFRGRVQNRKEDLLDVFLIDCGNVLSVDIARISSCSDDLFILPPKIVSGFLANVLILQNCSHSVVERYLSSLSGKNVTGFIQAFLPHKVLLFEAPDINSDLVRHGFGRHVDRDTFLLLVSMLTEVPLKQNMEPVPDLLQGYQDMLSFCGPKFSFGTCSEVRVTAAVHPGLFYCQMANTAAELWQVSKKLAAICEDKTKGCSQKTPENLGVLCAVKGKDQRWHRGFVQLLPANSQVRVFFIDYGFFETAKIENVHSLPPEFYSMQMMAFPCTLPSLADQDLVFRTQQMSLLKAGLLGAVLDVEITGFDKDNHLYTITVLSAKDKHLEEPEPIQNLSSQPLSDVEEPSPQDRYENYEKILSEALYKTLETEEIQVDSFFMGYVEYAQDPNHFWIRTQKRNQELEEMMAKIGEHFSQVKLDEDVLLNPEVGAMCCALYNEDFHFYRGVVTDVLEHGAEILFIDYGNVEKVPHKLIKNIPKTFADTSPFAVCCTLVNIFPLDDVWPSATCQFFRKMVSGRALLVHLIQMKKYTFVVDLFEMGSDKSMTEVLISSKLAEYIPHVPVEKKQDVLDKARQLQCSETNMRKEQQQDCEGEVSLCRNKTEKIEVHSCYKSLKFKPGFEVSVRCSHINSPSDFWCQALVGTQALEKLMDDIQLHYSAHTVPLHPEESCCIVKSPEDGCWYRGLIIGNRKSQALVMLVDHGSIVLVTPSHLQAIMPEYLRLEGQAFRCSLYNLIEPADPKYCGDWSPHACNLLKGFALNSEGVLKCKISSLLIVKNKGMCNVVSLHNTQTQQSVANTLIKQGLATVATSSTRQNSDVSPESFIYSSFDISPGDEEQVYVTHVNSQCEVYCHLERNTEIIEELEIKILEATENMQARTGAVVRKMCLAKYLDGKWYRGVTLSVQSSLHVGVFFVDYGNTNISEKTSVMFISRSCEDLLYTPMQAVRFNLHSVPTEELYADALEWLINAVLNKLVRVVVRGKRDDGSFDVELFDGKININDKVKELILSLSQKPKTAVSFSVSCGKTKKQNQRIKCEIGTFKTLLKVHSASPSKAIGAKLKNAKNSALGSAPNRKARAKQELVRNQKRTGPIKPQIKSTSGNQHTRQTQHRKEKGTPQLVRLPYKNINPGCRVKCFMSHIDSTSRFYLQVSDDESAILKMGEDLNSSDFKDSLKPPSPLTVSDAVLSEFEEDGALYRSVVKNQEDDSSYKVEFVDYGNSAFVGKEKIYFLPEQFLSQPRFSIPCSLINTCTYRDEASFIEAVTEKPLLVEFVCQIGDQWQVKVELLDGEAERDATLNSTSAAKSTEATPSLLSDFTPSTSPCKKNLREESQQSEKYREAAATIKNKGLMPEPSPKQKVQITRWVRQKCYRRKRNRKFKTSSIPVSRDCPDVFIHQTVQARETETGTILSVQSDSSFYVRLTKENNLLTSMERHIKDNIKKYKTVPEKDVKQDLKCLVQAEDGKWRRAVVQQIRERKFEVFLVDHGITVEMPRGPVPQQCTDLKEVPNLALLCKINSVGLDEDPTPQCWYEMLKPLVGTEVKLILVQISEADGVWLVEIALSELLLMSQIKASLHKNGDKSALAAETQNEASPDGFTADTSTPQLLFFAPLETDKAYSGFASAFTTPFEFCVVLEDLLLVMDKVSIMLDNLPGNMPSLPEAHLVPGTCCLLKSESKNKWCRTQIIHTDTTVVLKLVDYGHHEVIPYHDLSKLKQLPVELANLPKMTYPCILRGVKPAGVDGQWSDEAAAFFQDCLYKKDLQIFFRECGPNSIWRVDIVADGVHIAKKLVDAGHANYMDILLGRRYTLQILLVVNYKMHDVDSLTKPCYVGSIFKIFICISLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPZ7</accession>
    <name>A0A3B5MPZ7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MPZ7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025858"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026169.1">
        <property type="protein sequence ID" value="ENSXCOP00000025858.1"/>
        <property type="gene ID" value="ENSXCOG00000019324.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163423"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000002">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000049">
        <property type="entry name" value="Voltage-dependent sodium channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF278">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN TYPE 2 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="392"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="482"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="9">
        <location>
            <begin position="32"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="9">
        <location>
            <begin position="281"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="520" mass="59562" checksum="EA72B18A00AA649C" modified="2018-12-05" version="1">KTYIFKNLELKITRVNTKCLLNNSNIIFLLCFAGLKTIVGALFQSVKKLADVMILTVFCLSVFALVGLQLFMGNLRNKCIIEPEDKNLTTNDTYINNENNQYFPPGKRDALLCGNSSSAGQCPEGYMCLKAGKNPDYGYTSFDNFGWAFLSLFRLMTQDYWEYLYQQTLRAAGKPYMIFFVLVIFLGSFYLINLILAVVAMAYEEQCQVNLKKAQEKEFTGQLTGKSSTEISCDVLTIELEESRQKCPPYWYKFADKFLIWECCPVWMKIKEIVKIIVMDPFTDLAITLCIILNTLFMAMEFYPMDKWFSDMLYVGNLVFTAIFTAEMVLKVIALDPYHYFQVGWNIFDGVIVCLSLIELSLADVKGMSILRSFRLLRVFKLAKSWPTLNKLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKHYKDCVCKISEDCTLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGKAPCLILYMTVMVIGNLVVHCIFFGHFIPHHSNGFNGTYFCNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQC3</accession>
    <name>A0A3B5MQC3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Contactin associated protein like 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the neurexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQC3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026614"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026938.1">
        <property type="protein sequence ID" value="ENSXCOP00000026614.1"/>
        <property type="gene ID" value="ENSXCOG00000019871.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160228"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000026">
        <property type="entry name" value="contactin-associated protein-like 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF40">
        <property type="entry name" value="CONTACTIN-ASSOCIATED PROTEIN-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 10" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="14">
        <location>
            <begin position="86"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="251"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="398"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="223"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="430" mass="48183" checksum="7E9D48775B8D2B05" modified="2018-12-05" version="1">MLRLNVLYIHVNLQAINEGAVLAFFSAYFSLFFYVFFSRTEDSGLVTHKESLPVRSLVLGDLQRPGSEAAYRVGPLRCYGDKNFWNAAFFDKETSYLHFPTFHGELSADISFFFKTTSSSGVFLENLGIKDFIRIELSTSTRVVFSFDVGNGPLEVKVESTVPLNDNRWHRVRAERNIKEASLRLDDLPVATQEAPADGHVHLQLNSQLFIGTASRQRGFRGCIRALQLNGVTLDLEERAEMTPGVRPGCPGHCSSYGSLCQNQGRCVERAAGFHCDCSLSAYTGVFCQTELSASFKPGTSVRYTFKEPYELTRNSSALPSSIYSDTTLRGENVSMSFRTAQSPALLLYVGSYYREYMAVLINKHGIIAVVIFVVLTGLAVAARYLYRRKDTYRNQEGNAVKQEDSPDFPFNNQTDSQNLSSENPTEYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQL0</accession>
    <name>A0A3B5MQL0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Complex I-B9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">NADH-ubiquinone oxidoreductase B9 subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFA3 subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQL0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026063"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026376.1">
        <property type="protein sequence ID" value="ENSXCOP00000026063.1"/>
        <property type="gene ID" value="ENSXCOG00000019470.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004322"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22902">
        <property type="entry name" value="NDUFA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026626">
        <property type="entry name" value="NDUFA3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15221">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15221:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14987">
        <property type="entry name" value="NADHdh_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="83" mass="9034" checksum="0BCED73E044CD1B9" modified="2018-12-05" version="1">MAGIGAFLKNAWNKEPVIVASCGIGLVGIILPFISPYTKYTAMINEATPYSYPVPVRDDGNMPDVPSHPSEAKGRSLEWLKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MR14</accession>
    <name>A0A3B5MR14_9TELE</name>
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        <submittedName>
            <fullName evidence="3">Si:dkey-87o1.2</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MR14"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026546.1">
        <property type="protein sequence ID" value="ENSXCOP00000026228.1"/>
        <property type="gene ID" value="ENSXCOG00000019590.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015721"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="85"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="183" mass="20745" checksum="6F92E5D48AA7EE9A" modified="2018-12-05" version="1">MRHPRAPQPECESSASYGYTSSLHGKLKPIHNSPAMKLVVFFVAVSVAVMVAMIFQTLRQELSLRNLRARMVESSAEVKRREDSIMDMKSKIQELKTTVDNANLKLEDLKKEKTEKEKALQESEKSLETCNAGKKKTELGESIKKLQTAKNKAQEEIGKLKQQILDREKTICAHADMTKEEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MU35</accession>
    <name>A0A3B5MU35_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MU35"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027273"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027606.1">
        <property type="protein sequence ID" value="ENSXCOP00000027273.1"/>
        <property type="gene ID" value="ENSXCOG00000020368.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009987"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029383">
        <property type="entry name" value="ARL6IP6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28640">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 6-INTERACTING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28640:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 6-INTERACTING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15062">
        <property type="entry name" value="ARL6IP6"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="141" mass="14814" checksum="FB8C268EC34A3979" modified="2018-12-05" version="1">MSTPGFSGSGRLRSSGLAPWLKATFSVLGSAVAMLAVGAFCALTYPILRGRHASDQKPVTSHVCVPGFWSILTISTVAGCISCIFSWSLTQLDSHQPGMAFPSLLSLLDFRDPSSHGFNVTSGLAVLNGILATLTLIWSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUQ9</accession>
    <name>A0A3B5MUQ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Serine/threonine-protein kinase 24</fullName>
            <ecNumber evidence="9">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="28">Mammalian STE20-like protein kinase 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">STE20-like kinase MST3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="32 33">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="25">Monomer. Interacts with CTTNBP2NL. Interacts with RIPOR1 (via C-terminus); this interaction occurs in a PDCD10-dependent and Rho-independent manner. Interacts with PDCD10; this interaction is required for the association of STK24 with RIPOR1. Part of the core of STRIPAK complexes composed of PP2A catalytic and scaffolding subunits, the striatins (PP2A regulatory subunits), the striatin-associated proteins MOB4, STRIP1 and STRIP2, PDCD10 and members of the STE20 kinases, such as STK24 and STK26.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MUQ9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027508"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027842.1">
        <property type="protein sequence ID" value="ENSXCOP00000027508.1"/>
        <property type="gene ID" value="ENSXCOG00000020534.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153476"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030336">
        <property type="term" value="P:negative regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048679">
        <property type="term" value="P:regulation of axon regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06609">
        <property type="entry name" value="STKc_MST3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000411">
        <property type="entry name" value="Probable Ste20-like kinase Don3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.70:FF:000002">
        <property type="entry name" value="Serine/threonine kinase 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000252">
        <property type="entry name" value="Serine/threonine-protein kinase 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046409">
        <property type="entry name" value="PDC10_dimerisation_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048288">
        <property type="entry name" value="PDCD10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20929">
        <property type="entry name" value="PDCD10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="18 29" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="30" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0418">Kinase</keyword>
    <keyword evidence="19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="21" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16 29" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="31">
        <location>
            <begin position="24"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="30">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00066233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00072153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00077261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00079554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="395" mass="44353" checksum="133E889DC15381BA" modified="2018-12-05" version="1">MAHSPVQGNLPGMQNVRVDPEELFTKLERIGKGSFGEVFKGIDNRTQKVVAIKIIDLEEAEDEIEDIQQEITVLSQCDSPFVTKYYGSYLKDTKLWIIMEYLGGGSALDLMEPGALDETQIATILREILKGLEYLHSEKKIHRDIKAANVLLSEQGEVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIKQSAYDSKADIWSLGITAIELAKGEPPHSDLHPMKVLFLIPKNNPPTLEGNYCKPLKEFVEACLNKEPSFRPTAKELLKHKLIVRHAKKTSYLTELVDKYKRWKAEQSRTTESSSDESDSADQTLSSLVFNFFPQTKDIPRRPYSQSLTTVISPALAELKARQEQVNGNPLVLDELRAAILLAEEAYPGISDSLVAHMVQRLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MV86</accession>
    <name>A0A3B5MV86_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Dickkopf WNT signaling pathway inhibitor 3b</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dickkopf family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MV86"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027723"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028058.1">
        <property type="protein sequence ID" value="ENSXCOP00000027723.1"/>
        <property type="gene ID" value="ENSXCOG00000020672.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000221"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039706">
        <property type="term" value="F:co-receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048019">
        <property type="term" value="F:receptor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23274">
        <property type="entry name" value="Dkk3_Cys2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.80.10">
        <property type="entry name" value="Lipase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006796">
        <property type="entry name" value="Dickkopf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039863">
        <property type="entry name" value="DKK1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047300">
        <property type="entry name" value="Dkk3_Cys2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12113:SF8">
        <property type="entry name" value="DICKKOPF-RELATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12113">
        <property type="entry name" value="DICKKOPF3-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04706">
        <property type="entry name" value="Dickkopf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017225812" evidence="8">
        <location>
            <begin position="22"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Dickkopf N-terminal cysteine-rich" evidence="9">
        <location>
            <begin position="100"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="229" mass="25829" checksum="059B0E8808F64A1C" modified="2018-12-05" version="1">MSGTMLLLGFWALCLLWTAGAMERGPVSLNEMFREVEELMEDTQHMLDEAVHKVCVTFYPFISEKNFIIEIHETDNRTGETYTSHVHVEVSGQWNGVAHECMVDEDCGDLKYCLYEIENSRCLPCIATDMPCSKDEECCSDQMCVWGQCTVNATQGTAGTICQGQSDCRPDLCCAFQREFLFPVCSPKPLKGESCLNHPNLLMDMLEWDQEGPRDHCPCVANLHCQPHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVB6</accession>
    <name>A0A3B5MVB6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acidic leucine-rich nuclear phosphoprotein 32 family member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Histone chaperone that specifically mediates the genome-wide removal of histone H2A.Z/H2AZ1 from the nucleosome: removes H2A.Z/H2AZ1 from its normal sites of deposition, especially from enhancer and insulator regions. Not involved in deposition of H2A.Z/H2AZ1 in the nucleosome. May stabilize the evicted H2A.Z/H2AZ1-H2B dimer, thus shifting the equilibrium towards dissociation and the off-chromatin state. Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Multifunctional protein that is involved in the regulation of many processes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 11">Belongs to the ANP32 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVB6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027758"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028093.1">
        <property type="protein sequence ID" value="ENSXCOP00000027758.1"/>
        <property type="gene ID" value="ENSXCOG00000020697.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182907"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019212">
        <property type="term" value="F:phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000003">
        <property type="entry name" value="Acidic leucine-rich nuclear phosphoprotein 32 family member A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045081">
        <property type="entry name" value="AN32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11375">
        <property type="entry name" value="ACIDIC LEUCINE-RICH NUCLEAR PHOSPHOPROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11375:SF5">
        <property type="entry name" value="ACIDIC LEUCINE-RICH NUCLEAR PHOSPHOPROTEIN 32 FAMILY MEMBER E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="178"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="181"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="255" mass="27828" checksum="AA673A660C9EE4AC" modified="2018-12-05" version="1">MEMKKRISLELRNRSPAEVRELVVDSCRSADGEVEGVTEEFSALEVLSMVNVGLGSLAKLPALPKLRKLEVSDNSISGGLDALAEKCPNLSYLNLSGNHIKELSSLQPLQNLKNLKSLDLYSCGVTAADGYRDAVFRLLPQVVYLDGFDPDDNEVPESNGEFRVRFGPVLLVPCVTRCSVPDEDDEAGPPGDEDEDEDEDEDEEEGSEVGLSYLMKEGIQVSLAVVSSSEDDDALAQGEKRKRDADDEGDDDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVC7</accession>
    <name>A0A3B5MVC7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TFDP1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the E2F/DP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVC7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025257"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025562.1">
        <property type="protein sequence ID" value="ENSXCOP00000025257.1"/>
        <property type="gene ID" value="ENSXCOG00000018861.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154652"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14458">
        <property type="entry name" value="DP_DD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000047">
        <property type="entry name" value="Transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.80:FF:000001">
        <property type="entry name" value="Transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.80">
        <property type="entry name" value="Transcription factor DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037241">
        <property type="entry name" value="E2F-DP_heterodim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038168">
        <property type="entry name" value="TF_DP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014889">
        <property type="entry name" value="Transc_factor_DP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015648">
        <property type="entry name" value="Transcrpt_fac_DP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08781">
        <property type="entry name" value="DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009404">
        <property type="entry name" value="Transcription_factor_DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01138">
        <property type="entry name" value="DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144074">
        <property type="entry name" value="E2F-DP heterodimerization region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="11">
        <location>
            <begin position="108"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor DP C-terminal" evidence="10">
        <location>
            <begin position="198"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="79"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="365"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="101"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="365"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="386"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR009404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="401" mass="44706" checksum="1498F23758951318" modified="2018-12-05" version="1">AGLIETNAELKVFIDQNLSPSKVLSLVTVQPTVAPKQLLPKTLGPSNVNVAAHMQHVPHMVIGTPQRPTVSNTILVNSPHTPSSQFLTQTQPADASPWSSKRGKKGEKNGKGLRHFSMKVCEKVQKKGVTTYNEVADELVAEFSSSDNHMSPNDSHVYDQKNIRRRVYDALNVLMAMNIISKEKKEIKWIGLPTNSAQECQNLEVERQRRLERIKQKQSQLQELILQQIAFKNLVKRNKETEQQANRPPPPNSIIHLPFIIVNTSKKTVIDCSISNDFEYLFNFDSMFEIHDDIEVLKRMGMACGLEVGKCSPENLKIARSLVPKVLDPYVIMSKGPISNVYITGGSSANGGRYTADGCTDGTMASSSNDSHYSGSRVETPVSYMGDDDEEDEYEENDDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVK9</accession>
    <name>A0A3B5MVK9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVK9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023584"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023865.1">
        <property type="protein sequence ID" value="ENSXCOP00000023584.1"/>
        <property type="gene ID" value="ENSXCOG00000017620.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000180489"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="204" mass="21838" checksum="5F368F1895094707" modified="2018-12-05" version="1">VPTCSTVITSSKIPRTSSPITVPWLSSPVPKCPIGRSSSPVTIPFLSSPVPKTASPLTLPNKSTSLVLPKSFGAEAIPRNSSPVTLPRLSSPVTVPKMDSWVPNGSPQLCRKTFTTSGTSSPRASPVSLATVPMNTLYSPENTGDVLDLTVCLCWHLVWLTLLTVNICVFPDQWFPAGQCSGQTNCDPNQGNLSVFKINHLFKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZ74</accession>
    <name>A0A3B5MZ74_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small-subunit processome Utp12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat PWP2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MZ74"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027417"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027750.1">
        <property type="protein sequence ID" value="ENSXCOP00000027417.1"/>
        <property type="gene ID" value="ENSXCOG00000020474.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074981"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001388">
        <property type="entry name" value="PWP2 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000541">
        <property type="entry name" value="PWP2, small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001231">
        <property type="entry name" value="PWP2, small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027145">
        <property type="entry name" value="PWP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19858:SF0">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19858">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="184"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="371"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="413"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="468"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="6">
        <location>
            <begin position="741"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="226"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="852"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="240"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="256"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="858"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="883" mass="98331" checksum="C9A7C18B22364755" modified="2018-12-05" version="1">MKFAYRFSNLLGAVYRQGNLSFSKDGNAVISPVGNRVSVFDLKNNTSETLPISTTKNITCVGVSPDGSLAIVVDEDGAALLVSLITRAVLHHFHFHKPVSSIRFSPDGRKFVVTKENVALLYHAPGKQREFNAFVLDKSYYGPYDETTCIDWTDDSKCFVVGSKDMSTWVFGAERWANLIYYSLGGHKDIIVGCFFEKDSLDLYTVSQDGALCVWESDTELDGLVLKKSSANPGPRRQEGDEEEEDEDRVEGEEAEVIRGKTEPPKDKKQVKNVRYKHRSKHFFNKEGEFNNLTAAAYHKPTHILVTGFASGIFHLHELPEFNLIHSLSISDQRIASVAVNSSGDWIAFGCSRMGQLLVWEWQSESYVFKQQGHFNNMASLAYSPDGQYVVTGGDDGKVKVWNTNSGLCFVTFTEHTSSVTNVTFTSSGFVIVSASLDGTVRAYDLHRWAPRFLRDLSLVHADGQTVLGGHEGPVSSLCFSPVQSILASASWDRTIRLWDMLDSWQVKETLLLTSDGLCVTYRPDGQELAVATLNGEISFWNPQTAAQTGSITGRHDLETGRKETDKITAKQSAKGKSFTALCYSADGESILAGGHSKFVCIYNVKEQMLLKKFEISCNLSFDAMEQFLDRRKMTEFGSLALVDEGAGDGDGVDISLPGVRRGDMSSRHFKPEIRVSSLRFSPTGRSWAATTTEGLLVYSLDGSLVFDPYDLDLDVTPASIRKQLRLQDWASAIVLAFRLNENALKQEVLEAVPHEQIPVVCGSLPDVYVEKLLGFVATCLEKSSHLQFYMTWAQNLLMLHGQKLKNRSASILPTLQSLQKSIQRHSDSLSKLCDFNMYNIRYAAALSKQKGIKRPMEEEEEPDEMMSEEMSEASLEESDMML</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRM2</accession>
    <name>A0A3B5KRM2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Mobilizes copper(1+) out of the endosomal compartment, making copper(1+) available for export out of the cells.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Ag(+)(out) = Ag(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:75207"/>
            <dbReference type="ChEBI" id="CHEBI:49468"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Cu(+)(out) = Cu(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:75211"/>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Apical cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Basolateral cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Early endosome membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Membrane</location>
            <topology evidence="22">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 22">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRM2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000535"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000544.1">
        <property type="protein sequence ID" value="ENSXCOP00000000535.1"/>
        <property type="gene ID" value="ENSXCOG00000000466.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155147"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF22">
        <property type="entry name" value="HIGH AFFINITY COPPER UPTAKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 22" id="KW-0186">Copper</keyword>
    <keyword evidence="12 22" id="KW-0187">Copper transport</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="15 22" id="KW-0406">Ion transport</keyword>
    <keyword evidence="17 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0558">Oxidation</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 22" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00037299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="137" mass="15440" checksum="A44636129ED7D655" modified="2018-12-05" version="1">MKFKNVVVGKHFRKQLVINTKASSSSLLFLEVGKEQLVYRMMVRFPLMQRMLSHAHFLQTVLHIVQVVVSYVLMLVFMTYNAYLCIAVAAGAGMGYFLFSWQKAVLGSVHQVVQVLLRFESSLPPGSRSRSMPASAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KW67</accession>
    <name>A0A3B5KW67_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CASP8 and FADD-like apoptosis regulator</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C14A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KW67"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001861"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001889.1">
        <property type="protein sequence ID" value="ENSXCOP00000001861.1"/>
        <property type="gene ID" value="ENSXCOG00000001502.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064199"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000016">
        <property type="entry name" value="CASP8 and FADD-like apoptosis regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001875">
        <property type="entry name" value="DED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48169:SF3">
        <property type="entry name" value="CASP8 AND FADD LIKE APOPTOSIS REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48169">
        <property type="entry name" value="DED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01335">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00031">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50168">
        <property type="entry name" value="DED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="DED" evidence="4">
        <location>
            <begin position="6"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="DED" evidence="4">
        <location>
            <begin position="102"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p20" evidence="5">
        <location>
            <begin position="236"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="434" mass="50069" checksum="C21AEC0C3FB0DBE9" modified="2018-12-05" version="1">MSPFEEYDLRINAIAEDLTIGERKQAIYLCESLITDSSVACVRETLRVKVNFHADPPLFIMSILRQLGRYDILKRVYKVGRNEIEKKIQTYRQVLPRFSLSFCRVLMVTISDNLQMEDVEQIKFLLGRTLSHEKTENIKTFLEVMIELEKLDSVSPERVELVEDCLRNIGRLDLAKIVAYLNAPNDSRGALKKREVKTNNFGGDSHDIQSDCLLSDHNVFFIFMFIHIVFSSSGMLENTFKALHFNVLYHSMLGANEIFETLKEVSKHRQSRNEDAFVCCIISRSLDDNILGTDSYGRGLPVKNIRDLFLGATCPKMVGKPKLFFIQSYVVPESDHLEQDDCDSLPMRENLPTDADILWSHCRTDESQLLQEQHHSVYLKALTDALSKAKRWETDLLDVQMKVNGAIYDHNQKHPEANYHCDVKHTLRKSLYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KWI6</accession>
    <name>A0A3B5KWI6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1</fullName>
            <ecNumber evidence="15">2.4.3.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Gal-NAc6S</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">ST3Gal I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">ST3GalA.1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">ST3O</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Sialyltransferase 4A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.3.4" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KWI6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004483"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004539.1">
        <property type="protein sequence ID" value="ENSXCOP00000004483.1"/>
        <property type="gene ID" value="ENSXCOG00000003539.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154725"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003836">
        <property type="term" value="F:beta-galactoside (CMP) alpha-2,3-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901137">
        <property type="term" value="P:carbohydrate derivative biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097503">
        <property type="term" value="P:sialylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000015">
        <property type="entry name" value="Lactosylceramide alpha-2,3-sialyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051757">
        <property type="entry name" value="Beta-gal_alpha2-3_sialyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012163">
        <property type="entry name" value="Sialyl_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46032">
        <property type="entry name" value="ALPHA-2,3-SIALYLTRANSFERASE ST3GAL I ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46032:SF6">
        <property type="entry name" value="CMP-N-ACETYLNEURAMINATE-BETA-GALACTOSAMIDE-ALPHA-2,3-SIALYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005557">
        <property type="entry name" value="Sialyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="binding site" evidence="23">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="132"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00042682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00042991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR005557-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR005557-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="323" mass="37412" checksum="9AA24A55D41BE737" modified="2018-12-05" version="1">MDFYIKYKFKERSLCYDSSRTFKVKLLVLLLALTAAVFLKSDLTDWLWLQDQNSCACGKRLSKDNLRALHRLNHSVEPFLTVNTNLSEEAFRWWTHEQRDFSYYKSTVHKLFELLPPVADHGEPSSDSCRTCAVVGNSANLKGSRYRSLIDYQDVVIRMNAAPTKNYESDVGTKTTHRVMYPESAVDLDNSTLLVLFPFKINDLQWVIKAITTGFYGRSYVPIKFKIKANKDLVSFMRYVHEIWLKKKGDYPFTGFMALILALQICDEVSVFGYGADDDGNWSHYFETLKSKNFKTGPHPGKHEYAIIDEVAQNKTIRLYNKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KX80</accession>
    <name>A0A3B5KX80_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Postsynapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TANC family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000002236"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002268.1">
        <property type="protein sequence ID" value="ENSXCOP00000002236.1"/>
        <property type="gene ID" value="ENSXCOG00000001777.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155655"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058018">
        <property type="entry name" value="AAA_lid_TANC1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050889">
        <property type="entry name" value="Dendritic_Spine_Reg/Scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058056">
        <property type="entry name" value="WH_TANC1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166:SF23">
        <property type="entry name" value="PROTEIN TANC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166">
        <property type="entry name" value="ROLLING PEBBLES, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25520">
        <property type="entry name" value="AAA_lid_TANC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25521">
        <property type="entry name" value="WHD_TANC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 9" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="TANC1/2-like AAA+ ATPase lid" evidence="11">
        <location>
            <begin position="565"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="TANC1/2-like winged helix" evidence="12">
        <location>
            <begin position="662"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="867"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="987"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="1020"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="1053"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="1086"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="8">
        <location>
            <begin position="1119"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="1245"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="89"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1334"/>
            <end position="1358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1422"/>
            <end position="1511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1524"/>
            <end position="1587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1597"/>
            <end position="1616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1636"/>
            <end position="1703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="259"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1542"/>
            <end position="1555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1600"/>
            <end position="1610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1637"/>
            <end position="1647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1650"/>
            <end position="1661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1703" mass="184011" checksum="FDD960E27F52CADD" modified="2018-12-05" version="1">MPCIQQRGSGQRATHHHGRNGSHLNRKRLTKGVSISLPSSPLLPRQGDIVSSQSCVKFQDLQVEPHLGSSLSTQELMTRLCFLLGEATGSTASSPMEDRREKKVGCSLPQSLPLSSPHRGSSSSPCGTLSSPAHSPTPCPTSGLISVSSPGPPTQSPTSTLESKDSGIIGGYATYVHSCILRLVSSRSLNLCLYLFMPHISMDTVIDLHICSMQVFFFYLCTFPACFSHTELFPTHSLCTATSSTRLEDLSYLDNQRASGHRGSVRKHNTAGRTSDDSKGRLVPFKQTDIMLKPLLFEVPGVTAETPFVGRDWLFARLEEVLRKTSTCEGRGAVVVGNAGSGKTAIIWRLVTLSCHGMRTPQGAASDPHSPKSSPKWRFIIFFSLLPPMSGTETVLLSEQCEDSPLMRVDCAHKCTVVAYHFCQADNTYTCLVPEFVHSVAALLARAPQLAPYRELLAQEPHLQNQLSLRSCVQDPIAAFRKGVLEPLVSLRKERRLPEEDYIILVDSLNEAEFHKPDYGDTVATFITKIISKFPHWLKLVVTVRTGLVEITTLLPFAGISLDILPDSSDLGADLSDYIHHRVSGSSQIAANVTTPTGSAPDPLLLSKFSSNLSTRSHGSILYLQLTLDLFHKGQLALKGGNYLVVPVSLSEVYLLMCRASFPDNDVFEQVLPVLNVALATLHPLTDEQMFRALNAGSVKGELEWNDFQQRLDSLAGFTVRPLIAQTIKLLHACVYRSGHALLAFMFSRQEGKLNRQQTMELGHHILKAHIFKGLSKKTGVSSSVLQAMWVNCSTDSLSAALGSLRNLYTPNIKVSRLLMLGGASVTWCTEVIGHAPILAVHAHLGHVEMVALLLEMGAPVDGTTGSGMTPLCLAAAAGHADIVSLLCKKGAKVGHADKSGQCALVHAGLKGHAEITGILLGQDWGADIPADPQQQHGNETTTGKTQAAQQAVTAAASVGHTQVVKSFLDLKDEQLAVQMDAHDSLWGETVLSAAAGRGRLEMCVFLLEHGAELEVPNRRGMVPLLSAAKHGHTQVAELLLKKGSDINIGDKQGRTALILAASEGHAATVELLLAKGASMSSTDQEGLTALSWACMKGQKGVVQLLVEAGAELNQPDRQGRTPLDLAALNGDADTVHSLVEHGAVLERSDNGGTRGSDRPAGCQNPALVASLLKKGSKTSHATWAMASSKPDILLILLQKLMEEGNILYKKGRMKEAGQRYQYALRKLPRESQGDELKGLKDLRVSLYLNLSRCRRKTNDFGIAEEFATKALELKPRSYEAHYARARAKRSSRQFAAALADLHEAARLCPSNREIRRLLVRVEEECKHHQKLVGNNAAPGYNRDPHAHHHQPTEEEADVYSQGRDEVGLHCGKSPEFWPLNPYGPNRTLPGGVTFGLPDQSPCFPPDDFVQNQLFVDASEPAHGMSRQTQSQSSRTHHNCHSLQSGSRGAVRPMSLCGPSSPLPGRHISTSLSPTEHYHPLSPNSSSPPGPLQMFQPRSSSFSRGSDRLSAHSGPLESFALALGSGLDGRRDGGGGGGTAGSRGSSSSQASSGNLSDGGGRQPAPDLAPCPIKSSGSFKAKPELKPRPFMGVMDKAVRVQSQQSSSMSRQGPGGAESFGMSVMEFQGLSSEFKRASFQEQLSSSQLQAREFGERTNQRESRSAASSQLLFPEGRHRQASLTRDNPALHMAPIKPKRSFIESNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXZ8</accession>
    <name>A0A3B5KXZ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Oxidative stress-induced growth inhibitor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Monooxygenase catalytic activity. Involved in regulation of cytokinesis; promotes RHOA activity, probably acting locally at the midbody in late cytokinesis. Monooxygenase activity is involved in stabilizing transient structures between daughter cells, termed intercellular bridges, before abscission. Regulates differentiation and proliferation through the regulation of cell death.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the OKL38 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXZ8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000429"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000437.1">
        <property type="protein sequence ID" value="ENSXCOP00000000429.1"/>
        <property type="gene ID" value="ENSXCOG00000000352.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006658"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030308">
        <property type="term" value="P:negative regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000152">
        <property type="entry name" value="Oxidative stress-induced growth inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029731">
        <property type="entry name" value="OSGIN1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15192:SF15">
        <property type="entry name" value="OXIDATIVE STRESS-INDUCED GROWTH INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15192">
        <property type="entry name" value="PROTEIN CBG05349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="338"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="490"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="500"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="513" mass="56185" checksum="2A96CC5A35CFB053" modified="2018-12-05" version="1">LPVYCQVTLEAVLSSCCLGNGPSGICLSYLLSGYAPYLSVEGCHPNPLLHSKLSERPHLSLLEQDLEYLCEGLEGRSSNPVAVLFDSLLLPDSDFGLDHTSPLEWRYEPERAVPHLVLGKGPPGGAWHAMEGSMLTLSLANWMELPGLKLKDWMRDKRRNVRNDRATPAEIASYYEHYVSQMNLEQNFACGTTVTAVTRESGQQDGSPPCWRVSGLQRRDNGCSVTEEVPFSLLAHNVVVATGTHDVPARLGVEGESLPFVCHSFWELEAAIARGELDQSSDPVLVVGAGLTAADAVLTAHHLNTSVCHAFRRSVSDPGLIFNQLPKLLYPEYHKVHQMMTQQQHQSHPLDRAQNLRRAPSSSSSSPSSYPGYLSFPCHRVAAFRPDRKCVLESDSGEQTVVRVSKALVLIGAHPNLSFLRDNGRSLGIDPEEPISCRRNPIDVDPITNMVLAADGPGMYAMGPLVGENFVRFLKGGALAIAGDLWKRRSGGQEAVTPDSRVDRQRDARVLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZA0</accession>
    <name>A0A3B5KZA0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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    </dbReference>
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    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
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    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="18"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="218" mass="24589" checksum="9538F613EA3FC243" modified="2018-12-05" version="1">MEKSRLYRLLYYFILNSPLVIYADATFTEVPKDVSVGEGEDVEMPCAFKALSSAPMSLEIQWWYIKMDGPKEGPDELQNRAKVPAAPLREATKISTVRIQGNAISHRLSLSKVKKEDGGVYECRVSDLWAEEDQDFTVHATLTITAGRGMVAEEAVSHIQNRWSGLRSPQCHICWKNGPCNVSNSRPHTHTHTHPHIRRHSTNSVATLLCLLLFCCRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZD2</accession>
    <name>A0A3B5KZD2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">PHD finger protein 14</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="32473.ENSXCOP00000003912"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003955.1">
        <property type="protein sequence ID" value="ENSXCOP00000003912.1"/>
        <property type="gene ID" value="ENSXCOG00000003067.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:histone reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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        <property type="entry name" value="ePHD_PHF14"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PHD finger protein 14 isoform X1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
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        <property type="entry name" value="EPHD"/>
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        <property type="entry name" value="Histone_Mod_Regulator"/>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF150">
        <property type="entry name" value="PHD FINGER PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PHD_2"/>
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    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="FYVE/PHD zinc finger"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZF_PHD_1"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="7">
        <location>
            <begin position="283"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="346"/>
            <end position="463"/>
        </location>
    </feature>
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        <location>
            <begin position="690"/>
            <end position="744"/>
        </location>
    </feature>
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        <location>
            <begin position="22"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="664"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="770"/>
            <end position="850"/>
        </location>
    </feature>
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        <location>
            <begin position="594"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="22"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="170"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
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        <location>
            <begin position="666"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
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            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="801"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="850" mass="94982" checksum="4235B3E3DD963D0F" modified="2018-12-05" version="1">VDRSLKRRQVKPLAASLLDALDYDSSDDSDFEVEDASGSDGTGNGSDEEGSKGSAAGSESDSENAISGNDGIDEEAKDVAVDDNLTEDEEKLKQQLSEDSSTKDIPSATPSKGRRKSKKTSEPESAAAGETESTSTSGGGVVTVDESLTEPKKWNFRRNRPHLDFTTMEELNEIDDYDSEDDNDWRPTAEKRKGKTSTQKDDDDDDDNDDDDEDDDEDDEGKDNGDDINNSSSDSDKEMKKPKKKVKSTNAFDDELTNDSMSQGKGNEDASLDRSQTWNTQHILICCVCLGDNSEDADEIIQCDNCGVTVHEGCYGVDGESDSIMSSASENSTEPWFCDACKNGVTPSCELCPNQDGIFKETDAGRWVHVVCALYVPGVAFGDIDKLRPVTLTEMNYSKYGAKECSFCEDARFARTGVCISCDAGMCRSYFHVTCAQREGLLSEAAAEEDIADPFFAYCKQHADRFDRKWKRKNYLALQSYCKVSLQEREKQLTPEAQARITTRLQQYRAKAELSRNTRPQAWVPREKLPRPLTSSASAIRKLMRKAELMGISTDIFPVDTSDANASMDGRRKHKQPALTADFVNYYLERNMRMIQIQDNISEQKNLKDKLESEQEKLHVEYNKLCEFLEDLLSVNGTLRSEGQAMWTLLGGIVGQKLNTPAILKAPKERKPSKKEGGTPGKSSSLPAILYSCGICKKNQDQHLLVLCDTCKLYYHLGCLEPPLTRMPKKTKNSYWQCSECDQASSDEADIAMETLPDGTKRSRRQIKGPIKFIPQEMSPEPKKQQVRGTRTRGQKRKRVPITEEREEKEPLPRERRQRQSAVQKKPKADDTRTECSTCKGPGDNENLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZS7</accession>
    <name>A0A3B5KZS7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Forkhead box O3b</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_028039725.1"/>
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        <property type="nucleotide sequence ID" value="XM_028039726.1"/>
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        <property type="gene ID" value="ENSXCOG00000004347.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000161401"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:lymph vessel development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20060">
        <property type="entry name" value="FH_FOXO1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Forkhead box protein O4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1690">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032067">
        <property type="entry name" value="FOXO-TAD"/>
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    <sequence length="659" mass="69976" checksum="190EA49699BE6223" modified="2018-12-05" version="1">MAEAPLPDTLPDLDVVIDPDFEPQKRPRSCTWPLPRPDSSAVKPESTEADIIPEEEDDEEDSATPTAITVNGSAAATEDQSSNSPITDGAFPSPGHDSGGSPLSTHSPTATSGALTPSGLPAAQTPRKASSRRNAWGNLSYADLITKAIESSPEKRLTLSQIYDWMVRSIPYFKDKGDSNSSAGWKNSIRHNLSLHSRFIRVQNEGTGKSSWWMINPEGGKGGKAPRRRAVSMDNSNKYTKSARGRAAAKKKAALQAVAAAAGEGGGDSPTGPSKWPGSPTSRSSEELDAWTDFRSRTNSNASTLSGRLSPILANPELDEVPDDEPPLSPMIYSSPGRALSPGNTNGKAVPTELPRLADLANTMTLNDGITQDLMDDFLDNIKLVPTTCSQAMQNGSSGFSFGSKPNGIGSPSSTSSPSSNNSSNGGGNSYSNSIFSPYTASSSLRPSPMQTIQENKQTSFSTTGMSHFGSHTLQDLLNSDSHNHSDVMMTQSDPLMSQASAVAIISQNSRRNVMVRNDPVMTFGTTGGLQSSQTNNHNQSTMRSLNGDLNLANEANTLANVKQQLLLSPIGGNGTSSMQIDTSIFLNGTASSSSGCQDRFPTDLDLDMFNSGSLDCDMESIIRNDLMDADGLDFNFESLANMNGVSNFTSTKHSWVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
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</entry>
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        <source>
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    <sequence length="480" mass="53978" checksum="EAAB466E1C336FA4" modified="2018-12-05" version="1">MSVNASQLELNQLIYRHLKEHGFLSAAEELQRHSPQVNTVLTDIMVHRLVTLVSAKKCAKLRKMLQTYETQISVCCVSAEVDVAKMETINALASESQPKKKRVRKPQAKPLSEADAPVQQNGETIGQNEQGIEKVGTQTPTKKNAAKRSKKESPAHVSSPTKQSPNKRVKSVEDKQASSELPEKFEKETELQEMLVPVNAKDAMAQETKKKKKKKKEKEKNEDKIEGSANETNSDGKDSKEKRSEITEQEEAGKTEGAKRTTKEGKMEERGSDDDNISELSLQKKKAKKKEKDKSSKNLKQPDKIIKPGKPKKKENNNMGGNLEYVSVGEEGSGVQTIEEIENSKEMVTKEKTSSEEYSAEMHKKKAKKKKDKSVMEENLEHGTMAVEENVGDEYNSEQIGGKKKSKKKKREPLGCKEFPEEVVQEKKAKRKNEKSDENDLVEEEKPKDDDDHTNNKCTLETDEGSLEITSPLKKKKSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
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        </subcellularLocation>
        <subcellularLocation>
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        </subcellularLocation>
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        <text evidence="10">Belongs to the ASPP family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ankyrin_rpt"/>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
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        <property type="entry name" value="ASPP1/2"/>
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        <property type="entry name" value="ASPP2-like_RA"/>
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    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24131">
        <property type="entry name" value="APOPTOSIS-STIMULATING OF P53 PROTEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR24131:SF5">
        <property type="entry name" value="APOPTOSIS-STIMULATING OF P53 PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ASPP2-like_RA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01887">
        <property type="entry name" value="SPECTRNALPHA"/>
    </dbReference>
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        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017339764" description="SH3 domain-containing protein" evidence="15">
        <location>
            <begin position="29"/>
            <end position="1074"/>
        </location>
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        <location>
            <begin position="904"/>
            <end position="936"/>
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    </feature>
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        <location>
            <begin position="937"/>
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        <location>
            <begin position="1003"/>
            <end position="1065"/>
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        <location>
            <begin position="98"/>
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        </location>
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            <begin position="339"/>
            <end position="525"/>
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        <location>
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        <location>
            <begin position="623"/>
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            <begin position="682"/>
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            <begin position="234"/>
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        <location>
            <begin position="104"/>
            <end position="120"/>
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            <begin position="484"/>
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        <location>
            <begin position="498"/>
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        <location>
            <begin position="629"/>
            <end position="639"/>
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        <location>
            <begin position="723"/>
            <end position="741"/>
        </location>
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            <begin position="848"/>
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    <sequence length="1074" mass="117516" checksum="D31EB3D9ED944794" modified="2018-12-05" version="1">MFSKFCSPGHLSLPCVFQMILTVYLSDGEQTAAEVPITPETTCRDVVEFCKEPGESGCHLAEVWRGNERAIPFEHMMYEHLQKWGPRKQEVKFFLRHEDSPTESSDQGSQQSQDQTGRRTGNAGEKHNENGVGNQRVELTLSELQEMATRQQQQIEAQQQMLVAKEQRLRYLKQQERRQQQTVSESEKLQRLKERVESQEAKLKKIRAMRGQVDYSKVINGNLSAEIEQVTSLFQEKQAELQTAVLRVEQLSLQLEDLRRGKLNGIQTGLGGQVTGAAALELRKLYQELQIRNKLNQEQNSKLQQQKELLNKRNMEVTLMDKRISELRERLYKKKAEARQKENLPLNRANGPPSPQPAPGTLGRVAAVGPYIQVPVPSRQDGGYTLPPDPLKPQTLGVNNQANQGRPKSANDTGWPTIGKSSTTLKPPERRDSGSDSQGKSPTSDPKLAVSSPAITKPQPPPYGSHLTSANTASSLERRKDAPPPRPLPNPPAPAWPRVPSSTGSSSQQIQQRISVPPSPTFQPNVPLYPPALSERLDHPPAVAVRPFIPDRGTRPQSPRKGPPTVNSSSIYHMYLQHAAPKSQPLKPAVKAVYGKPVLSSSSTPASPLPYVQAGGAFPALQGLAGSDDSVDGELDDPESFQPSAPPPSVENIPRPLSPTKLTPMVHSPLRYQSDADLEVLRKKLANAPRPLKKRSSITEPEGPSGPNIQKLLYQRFNTLAGGIEGNGGSGSGGSNGGGNGTPFYQPAIPPGYLGGDSLADTNNGNLLPETQLPPPPGREEPGSEASSTNDANDNEPNAPEGAADPGEEEEPEDDNNNNAGGSEASLPSPVPEVSSPEESGTAVSQPLEKRTNLKKPDSERTGHGFRVKFNPLALLLDASLEGEFDLVQRIIYEVENPSTPNDEGITPLHNAVCAGHHHIVKFLLDFGVNVNAADSDGWTPLHCAASCNSVHLCKLLVESGAAIFASTISDVETAADKCEEMEEGYIQCSQFLYGVQEKLGVMNKGTVYALWDYEAQSPDELSFKEGDAITILRRQDENETEWWWSRLEDKEGYVPRNLLGLCPRIKPRQRSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L202</accession>
    <name>A0A3B5L202_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Cullin 9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="9">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000004370"/>
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        <property type="protein sequence ID" value="ENSXCOP00000004370.1"/>
        <property type="gene ID" value="ENSXCOG00000003442.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="BRcat_RBR_CUL9"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
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    </dbReference>
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        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
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    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
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        <property type="entry name" value="APC_su10/DOC_dom"/>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
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        <property type="entry name" value="Cullin"/>
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        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
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        <property type="entry name" value="Galactose-bd-like_sf"/>
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        <property type="entry name" value="IBR_dom"/>
    </dbReference>
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        <property type="entry name" value="Rcat_RBR_CUL9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22771:SF4">
        <property type="entry name" value="CULLIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22771">
        <property type="entry name" value="CULLIN AND GALACTOSE-BINDING DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24742">
        <property type="entry name" value="ARM_CUL7_CUL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01337">
        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51284">
        <property type="entry name" value="DOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DOC" evidence="13">
        <location>
            <begin position="378"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="Cullin family profile" evidence="11">
        <location>
            <begin position="773"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="1182"/>
            <end position="1400"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="1186"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="12">
        <location>
            <begin position="1353"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="663"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="666"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="676"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1589" mass="177358" checksum="0D0149A972020B98" modified="2018-12-05" version="1">MRSVCLHVGDEVSCCLPAVFNNPRVQGKKSLLEKLGEVVDILKKGSSASEQTQQLATVLFMARDKVLKLLVELLGSSSRDVVMSTLRLTHLLMVKYEWRVCFATEGGVKAVLSCMQEFSTVVHVLQLALATLKVITGASKHDLRSFGSSLPLSESGTQMMLEIFASIGSATPEGSKGLLSAIPSAIDLMLKTKGCMLSVRNGLLVIIMLIASHKSLAEQLVACGVTTVLKKCLTLSRAETIPNMLGDQGPGEILNKFLDNYEEDMLPWHESIEPCLSTMTAFINDREVVQLFIRFLYRLASLNKDYAVVMCRLGTKESLVKALDKHSTNLLLVTELRDLISDCEKYASLYKKMTTSVLAGCIQMVLGQIEEHRRSHQPINIPFFDVFLRNLCQGSSVELKEDKCWEKVEVSSNHHRANKLTDKNPKTYWESNGCTGSRLFLGRGFVIQLAILVASEDSSYMPARILVLGGDDPANINTELNTVNVAASASRVVLLENMTRFWSIIQIRIKRCQQGGIDTRVHGFEVLGPKPTFWPVFKEQLCRRTYLFYSTKAHTWCQEVVEDKAQLLQLFNKLNSALRHEQMFADRFLPDAEAAEALGRTCWEALISPIVHSITLSASSPLSWLLSEYLENSEVARRCKSRAAIFNSRVRRLTHLLVHVDTSSADDGKEGKNKDSSAASSSSSSSSSKPKVKSSSSIAGIALCWQGVVQRQVKKFLESSCSLPDFVERYCTLYLRLKNAMEELFGQQTAFVLALRHGFSAALLQLSILRAMHVSERFAQYIDHMIQASAAPSGGAETLQRLQQFLEPMLFLSGLELANTFEHFYRYYLGDRLLAQGNVWLESAVIDQIGSCFPSRFPQQMLKNLSESAELQQEFHLYRLQQLDRHLQEQDQVGGRGLQGGSRERRRPPAEVQVLVLSPRCWAVSSGCFLVEPAKHFPAEICSYLNHFTQFYSHSDHSKPRRLQWTWLGHGELRLGGWTLHVSTLQMFVLLHFNESGLSAAVLLHALQPLIADGGPLTCSSEEEPSTAVWRMIWVLLQVMDSCQKQEAARSPGGGRFGCSTGDVLSCIMHVMNKGCVRRNDDNPHIVEFVPEDPATPQKGHAQISFGRCPAVCASLDVCPSHSVLMFPQVLATLSLDSDRAEHLLIHCRWNVDLLVQRYTDDPDALIMAAGLTCRNPQPPSPAPSCPVCLGPRAPDAEPVPTLSCMHYCCRSCWQEYLTARIEQNLVMNCNCPITDCQAQPTSQFFLGILTDRDTVAKYENALLRGYVECCSNLTWCTNPQGCDQILCQEAVGSVGTCSRCCWSSCFSCNFPEAHYPASCSHMSQWMDDGGFYEGMSTEAQSKHLAKLISKRCPSCQAQIEKNEGCLHMTCAKCNHGFCWRCLKPWKPTHKDYYNCSAMVSKAARQEKKFQDYNERCTFHHQAKDFAVGLESKVSSINEALQMKSLTFVIDACKVLAQGRKVLAYSCVYSYYNQETEKMDVMEQQTEALDLHTNALQILLEETLLQCTDLASCVRLLKPEHLNTGLELIRRIQERLLAILQHSTQVQPWRHPEGASGIGCCSQGAILEPFDPESLVVQFKPVSSVNSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L230</accession>
    <name>A0A3B5L230_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L230"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006437.1">
        <property type="protein sequence ID" value="ENSXCOP00000006358.1"/>
        <property type="gene ID" value="ENSXCOG00000004915.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177178"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="198" mass="21543" checksum="43393F25D8FA8B65" modified="2018-12-05" version="1">IRPISNTTEIFDTGLEAASLSFLASNLGLKSNTGSLNENIWKRTASHILVAFGVSVLISSLCRDHPLAFDFIPFDFLPFLSGLSAEHGSVLFLLFSVDLVEGASIGGVGSADSDFEHSCLICVTGVFGSIMRDLSAFTSDFAFSSSVCTFPLLKSFFTTFRESWSPLSRRESFVFVFVVTLVFSFWFSEGTATAFGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L294</accession>
    <name>A0A3B5L294_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphopyruvate hydratase</fullName>
            <ecNumber evidence="4">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L294"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006433"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006513.1">
        <property type="protein sequence ID" value="ENSXCOP00000006433.1"/>
        <property type="gene ID" value="ENSXCOG00000004983.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182805"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.120:FF:000002">
        <property type="entry name" value="Enolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF55">
        <property type="entry name" value="ALPHA-ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="9">
        <location>
            <begin position="65"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="357" mass="39133" checksum="8FCCE191F6E5A562" modified="2018-12-05" version="1">SLQDVSVVDQEKIDQMMIDLDGTENKSKFGANAILGVSLAVCKAGAAEKDVPLYRHIADLAGNPEVILPVPAFNVINGGSHAGNKLAMQEFMILPIGASTFKKAMRIGAEVYHNLKNVIKKKYGQDATNVGDEGGFAPNILENKEALELLKEAITKAGYTEEVVIGMDVAASEFYRDGKYDLDFKSPDDPSRYITPDELADLYRSFVKDYPVVSIEDPFDQDDWAAWTNFTESTDIQVVGDDLTVTNPSRICKAVEEKACNCLLLKVNQIGTVTESMRACKMAQESGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQILRIEEELGDKAQFAGKNFRSPVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L3I2</accession>
    <name>A0A3B5L3I2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L3I2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002334"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002368.1">
        <property type="protein sequence ID" value="ENSXCOP00000002334.1"/>
        <property type="gene ID" value="ENSXCOG00000001872.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234556"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000920">
        <property type="entry name" value="Myelin_P0-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13869">
        <property type="entry name" value="MYELIN P0 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13869:SF20">
        <property type="entry name" value="MYELIN PROTEIN ZERO-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00213">
        <property type="entry name" value="MYELINP0"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="41"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="217" mass="23916" checksum="370944A7F0630E66" modified="2018-12-05" version="1">MYFIDLEKFTADLFDTNYVLLSLSIITQPLRSHTHTHARTPTHACLISGVSRDLHATKGDTVKLPCTFTSTANPTSKMRVSWSYTPQTGGSSLFFHFSSKAELPKSGQFAGRIKWQGTPARGDVSISLINATLNDNGTYTCDVTNPPDVFDSPKSHTVLTVTPKATTIRFSDVAVLLAFILLPSGLITFFLIGRMFCPKKRHNQSKAYRSPIEVTEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L3X5</accession>
    <name>A0A3B5L3X5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L3X5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002469"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002503.1">
        <property type="protein sequence ID" value="ENSXCOP00000002469.1"/>
        <property type="gene ID" value="ENSXCOG00000001972.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164843"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="20"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="279" mass="30342" checksum="447B2842A44341C5" modified="2018-12-05" version="1">MNFDQILAAVGGFGKYQKILYIWICLPQILLAFHMMVSIFTGATPPYRCRESSSPGAGNQTSLPAENASLSESSCFSSEVMLRPQGNRTERVPCGRGWVYSMEVFHSTTVTEWDLVCSKAGLNSLGSSIYMFGLLVGSVLFGAMADRYGRRLVLLLSIALQTVFGVAVAFAPNFPVYAILRFAVGTTVSGVIINAFVLGTEWTCTKRRMLAGIITDYAFGLGYMLLAGVAYLIRDWRKLQLAISAPGFLKYLGQSDVCSVCSDSAVVVLHRHQDVAVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4I1</accession>
    <name>A0A3B5L4I1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Transcription factor 7</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCF/LEF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L4I1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005300"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005361.1">
        <property type="protein sequence ID" value="ENSXCOP00000005300.1"/>
        <property type="gene ID" value="ENSXCOG00000004153.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159831"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990907">
        <property type="term" value="C:beta-catenin-TCF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010456">
        <property type="term" value="P:cell proliferation in dorsal spinal cord"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060847">
        <property type="term" value="P:endothelial cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030901">
        <property type="term" value="P:midbrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021903">
        <property type="term" value="P:rostrocaudal neural tube patterning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21996">
        <property type="entry name" value="HMG-box_TCF7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000001">
        <property type="entry name" value="transcription factor 7 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000002">
        <property type="entry name" value="transcription factor 7-like 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013558">
        <property type="entry name" value="CTNNB1-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024940">
        <property type="entry name" value="TCF/LEF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10373:SF33">
        <property type="entry name" value="TRANSCRIPTION FACTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10373">
        <property type="entry name" value="TRANSCRIPTION FACTOR 7 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08347">
        <property type="entry name" value="CTNNB1_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="HMG box" evidence="12">
        <location>
            <begin position="248"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="10">
        <location>
            <begin position="248"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="370" mass="41778" checksum="1C0A78FD809E8109" modified="2018-12-05" version="1">MPQLSSGGGDDLGANDEMIAFKDEGEQEEKTQENAFTERDLADLKSSLVNESEINQSPTSAVRRGHHGEQRVFQDKHREHLDGVSKQQDGGMYKSPAYPGYPFLMLPDPYLPNSSVSPSSNKVSVVQQSHGMHPLTSLLPYSNDHFSPSPPHLPTDMSQKTGVHRHQSQDLSGYYSLPPGGVGQITPSMSWFPHSLMLGPSGVHPTGIPHPAIVPSSGKQEHDQYDRGMYMKPQVEVKREKEPKKPVIKKPLNAFMLYMKEMRAKVIAECTLKESAAINQILGRRWHALTREEQAKYYELARKERQLHMQLYPTWSARDNYVSYFALFLPLPNLFPSNSLAQSERIQTKELIKKNINRKVLQPLLSVVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4J8</accession>
    <name>A0A3B5L4J8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 4 member 11</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L4J8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005742"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005808.1">
        <property type="protein sequence ID" value="ENSXCOP00000005742.1"/>
        <property type="gene ID" value="ENSXCOG00000004467.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154894"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050801">
        <property type="term" value="P:monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000002">
        <property type="entry name" value="Solute carrier family 4 member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF127">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 4 MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01231">
        <property type="entry name" value="HCO3TRNSPORT"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="276"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="353"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="400"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="459"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="484"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="529"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="557"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="6">
        <location>
            <begin position="53"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="590" mass="66933" checksum="13AC0029138FC3BF" modified="2018-12-05" version="1">MHCLASTKTGRGSYLTSYTDFSDKFSLLETTERRRKWKKSFFKYMSDFFKAGRGIYEDLRRRLPFYPSDFTDGIVGSNKTLLKYMTTAIFLYIAILLPAIAFGSLNDESTRGEIDVRKTIIGQSIGGVIYSLFAGSPLVIPLTTAPLAIFISVIRGICDDYNLDFPAFYACIGLWNCLFLILGGIFNVSLLMKLFKRSTEEVIALFISIAFVVDAVKGTVKIFQRYYHAPGLENRSLEDLPQVGDLQGGNRSEVMVGSVFNALPESFIQCTRERPVLCLLLMLGTLWMGYTLYQFKRSPFLHAKVREVLSDCALPISVLLFSFIGSYIFSDIELPVFKVHNRPIFNVAPFERLSAMNVVSAMGLGFLLALLIFIDQNIVVSLTNAPENRLLKGTAYHWDLMLSGLINILMSVLGLPWMHAAFPHSTLHVRQLAFVEQRVEGGHLYETIVQVKETRLTSLAANIFIGVSLLLLPVPLQWIPKPVLYGLFLYIALTSIDGNQMCDRMALLLKEQTSYPPTHYIRKVPQRKIHYFTFLQMMQLLVLCTFGMYPIPYMKMIFPLVMILLIPIRNNVLPHIIEAKYLDIMDAQHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L554</accession>
    <name>A0A3B5L554_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ubiquitin-ribosomal protein eL40 fusion protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the eukaryotic ribosomal protein eL40 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027883153.1">
        <property type="nucleotide sequence ID" value="XM_028027352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L554"/>
    <dbReference type="SMR" id="A0A3B5L554"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007438"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007534.1">
        <property type="protein sequence ID" value="ENSXCOP00000007438.1"/>
        <property type="gene ID" value="ENSXCOG00000005243.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114150720"/>
    <dbReference type="KEGG" id="xco:114150720"/>
    <dbReference type="CTD" id="7311"/>
    <dbReference type="GeneTree" id="ENSGT00940000153593"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000014">
        <property type="entry name" value="Ubiquitin-60S ribosomal L40 fusion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.50:FF:000001">
        <property type="entry name" value="ubiquitin-60S ribosomal protein L40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001975">
        <property type="entry name" value="Ribosomal_eL40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="Ribosomal_eL40_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01020">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01377">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="11">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="128" mass="14742" checksum="7BDE802ABEFAD02A" modified="2018-12-05" version="1">MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGIIEPSLRQLAQKYNCDKMICRKCYARLHPRAVNCRKKKCGHTNNLRPKKKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5U9</accession>
    <name>A0A3B5L5U9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Mitochondrial ribosomal protein S22</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">MRPS22</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5U9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006192"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006263.1">
        <property type="protein sequence ID" value="ENSXCOP00000006192.1"/>
        <property type="gene ID" value="ENSXCOG00000004783.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006095"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019374">
        <property type="entry name" value="Ribosomal_mS22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13071">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13071:SF4">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10245">
        <property type="entry name" value="MRP-S22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="329" mass="37934" checksum="5749B49284386BE6" modified="2018-12-05" version="1">IILHNYILTLFTFQLGHFPLKYFTSFFVSHMYLHSPRAGAKPRFTDAAVQDVLSRITGLDLQKVFRPIKQELKPPTYKLMTDEQLEQAIQVATEEAKKQLQMPPVLPERKPINDVLAVDKILDGMDTAKYVFTDITYNIPHRERFIVVREPDGTLRKATWEERDRFVQVYFPKQGRKLTPHVLFKEENLKTVFSQDRHEDVLNLCLVQFEPDSADYIRVHEATYEDLEKNGKYNLLRSTRHFGGLAWYLVNARRVDGLIVDMLRRDMLQDAISLVSLFYMVHPHSESAQEAAGQQATGTDLLKVRTTQSGYIDLALQAYEEKAAEGSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L651</accession>
    <name>A0A3B5L651_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L651"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007891.1">
        <property type="protein sequence ID" value="ENSXCOP00000007793.1"/>
        <property type="gene ID" value="ENSXCOG00000005986.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155521"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000072">
        <property type="entry name" value="Protocadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000078">
        <property type="entry name" value="Protocadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000367">
        <property type="entry name" value="Protocadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF247">
        <property type="entry name" value="PROTOCADHERIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 9" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017442109" description="Cadherin domain-containing protein" evidence="11">
        <location>
            <begin position="23"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="12">
        <location>
            <begin position="20"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="12">
        <location>
            <begin position="133"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="289"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="364" mass="39759" checksum="58C963AE8E742071" modified="2018-12-05" version="1">MELNLRVVLFWVVVLHGGSVDGSILYRVQEEQPPNTLIGSLAADQGLPDSGHLYKLEVGAPYLRVDGKTGDIFTTEIPIDRETLRDCRSLAKGKPCYLEFEVSVTDLIQNQSPRLIEGRIEVQDINDNTPQFPSSVLAISVPENTIMGALFSIPLATDRDSERNGVADYALTAGPDAATLFGLQVAEDRGGKLPQLIVLGNLDRELKDSYDLTIKAVDGGNPQRYSSALLRVVVTDANDNAPKFEKTSYDAEVLENSPVGHSVLQHPHRRVTFSTANPVQDLQDASQHSYYDSGLEESETPSSKSSSGPRIGPLTLPEDHYERTTPDGSIGETEHPENGSVLNVMVCRVLCFSNFCTILCFSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6Q4</accession>
    <name>A0A3B5L6Q4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MAF bZIP transcription factor Aa</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6Q4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006507"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006587.1">
        <property type="protein sequence ID" value="ENSXCOP00000006507.1"/>
        <property type="gene ID" value="ENSXCOG00000005041.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162747"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14718">
        <property type="entry name" value="bZIP_Maf_large"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000016">
        <property type="entry name" value="MAF bZIP transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004826">
        <property type="entry name" value="bZIP_Maf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013592">
        <property type="entry name" value="Maf_TF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024874">
        <property type="entry name" value="Transcription_factor_Maf_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129">
        <property type="entry name" value="TRANSCRIPTION FACTOR MAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10129:SF30">
        <property type="entry name" value="TRANSCRIPTION FACTOR MAFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
        <property type="entry name" value="bZIP_Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08383">
        <property type="entry name" value="Maf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="221"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="53"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="311" mass="35170" checksum="B9F6BFC5423A3370" modified="2018-12-05" version="1">MATDLAMSAELPNSPLAIEYVNDFDLMKFEVKKEPLEADRFCHRLPSGSLSSTPISTPCSSVPSSPSFCAPSPGAQPNQGLPGGVNVGSNSNNSSGNNNHSNAGKPQLEDLYWIPSYQHHINPEALNLTPEDAVEALIGNAHHHHHHHQAYEGFRGQQYVGEDLSAASAVHHHQAHHHHHHHHHGHHTRLEDRFSDEQLVSMTVRELNRQLRGFSKEEVIRLKQKRRTLKNRGYAQSCRFKRVQQRHMLETEKCTLQNQVEQLKQDVARLVKERDLYKEKYEKLASRTYNSGGPANTRDPSGKQASTEFFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6V3</accession>
    <name>A0A3B5L6V3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 26</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6V3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003548.1">
        <property type="protein sequence ID" value="ENSXCOP00000003509.1"/>
        <property type="gene ID" value="ENSXCOG00000002772.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014794"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019169">
        <property type="entry name" value="Transmembrane_26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22168">
        <property type="entry name" value="TMEM26 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22168:SF7">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 26-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09772">
        <property type="entry name" value="Tmem26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="31908" checksum="BAAA5ECC571A4F31" modified="2018-12-05" version="1">LQQTFRARGGVNLDSCRTPCPQGLDLRTPGLDCGISFPQDIEELGPENWVAGLEQTMLIVLVLGRWLMPKGDMSRDQLSQLLMVYVGLGADILDIFDTFKEPEVKGNRVVIIVGLALFSWALMQFPLVLTQTRASQGDVPRSWNSLPCCHQPSSSLPSCCFNEIWSLLLTVGLQDAPFLVYRLYLIIEEQVQNQLMIFFTCKNLLIVLLELYRIFVVQFEQQVQESVLERCEALTTYCQTLGGSEVELGEEGRRKMGCSEEQSHCMDMDMEIEREEDHSVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8F8</accession>
    <name>A0A3B5L8F8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">GIT ArfGAP 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GIT2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="32473.ENSXCOP00000004079"/>
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        <property type="protein sequence ID" value="ENSXCOP00000004079.1"/>
        <property type="gene ID" value="ENSXCOG00000003201.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:presynapse"/>
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        <property type="project" value="GOC"/>
    </dbReference>
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        <property type="term" value="F:GTPase activator activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:small GTPase binding"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0036465">
        <property type="term" value="P:synaptic vesicle recycling"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="ARF GTPase-activating protein GIT1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARF GTPase-activating protein GIT2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
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    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt"/>
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    </dbReference>
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        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047161">
        <property type="entry name" value="GIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022018">
        <property type="entry name" value="GIT1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013724">
        <property type="entry name" value="GIT_SHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46097">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE INTERACTING ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46097:SF2">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE INTERACTING ARFGAP 2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12205">
        <property type="entry name" value="GIT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08518">
        <property type="entry name" value="GIT_SHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00555">
        <property type="entry name" value="GIT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
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    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="10">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="166"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="369"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="375"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="423"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
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            <dbReference type="PROSITE" id="PS50115"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="631" mass="70073" checksum="8D72E688D0D25B4F" modified="2018-12-05" version="1">MSKRVRSREVCADCSAPEPRWASVNRGVLICDECCSIHRGLGRHSSQVRHLTQCSWPQSQLQMVQTLYGNGANSIWEHSLLDPSSSASGKRKANPQDRVHPNKTEFIKAKYQMLAYVHRMPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHIAAKAGQILQAELLAVYGADPGALDSSGKTPIDNARQAGHQELAERLVEIQYELTDRLTFYLCGRRPGFFFTCSLDLSDFAKAAKKKLQSLSNHQFEELAMDVYDEVDRRETDAVWLATQNHSTLVTDTTVVPFLPVNPEYSSTRNQGRQKLARFSAHEFATLVIDILADAKRRQWGNSCDSPKDNVELILQGINSRHNSESQDNDQPDYDSVASDEDPVQEATCGDSCNHGRTKLSSLQTENTTLRWQTPGGQQHPQGPFGRHPPRGGRAMSMYETGSAPKHFSHRVDTARHDDGVVLQPFPTNVSTRCLYFCVCSSPEAAPDSVEPGVEGEEDATLPCTEDVICKTEQITKNIQELLRAAQETKHESFLPCSEKIRLAVTEMAALFPKRPSSETVRNSLYLLTLSASRLHGECQKAAELNPCPSDIQLVTQQVIQCAYDIAKAAKQLVTVTTKENNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAM7</accession>
    <name>A0A3B5LAM7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription factor 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAM7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009253"/>
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        <property type="gene ID" value="ENSXCOG00000007053.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:chromatin"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NeuroDiff_E-box_TFs"/>
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        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11793:SF10">
        <property type="entry name" value="TRANSCRIPTION FACTOR 4"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="62" mass="6699" checksum="C083B8C401CDC64B" modified="2018-12-05" version="1">MHHHQQRMAALGTDKELSDLLDFSAMFSPPVSSGKNGPTSLGSGHFSGSSTYHKPNLVIKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LB22</accession>
    <name>A0A3B5LB22_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Gamma-interferon-inducible lysosomal thiol reductase</fullName>
            <ecNumber evidence="7">1.8.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Gamma-interferon-inducible protein IP-30</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Lysosomal thiol reductase that can reduce protein disulfide bonds. Facilitates the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Dimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GILT family.</text>
    </comment>
    <dbReference type="EC" id="1.8.-.-" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LB22"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008027"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008125.1">
        <property type="protein sequence ID" value="ENSXCOP00000008027.1"/>
        <property type="gene ID" value="ENSXCOG00000005796.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164804"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016671">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004911">
        <property type="entry name" value="Interferon-induced_GILT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003119">
        <property type="entry name" value="SAP_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234">
        <property type="entry name" value="GAMMA-INTERFERON INDUCIBLE LYSOSOMAL THIOL REDUCTASE GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13234:SF45">
        <property type="entry name" value="GAMMA-INTERFERON-INDUCIBLE LYSOSOMAL THIOL REDUCTASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03227">
        <property type="entry name" value="GILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02199">
        <property type="entry name" value="SapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51110">
        <property type="entry name" value="SAP_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Saposin A-type" evidence="9">
        <location>
            <begin position="53"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="272" mass="30953" checksum="E0429CAF81AF5ABE" modified="2018-12-05" version="1">MTLSSSQEEGFSQSSLAVCLGAVWIFFLLRIMKVLCILAVLFLISTKHKSFELSHPKPACRYPPSQWCRSLEIAIECKVQKQCMELGAIKPNQTVPRVALTLYYESLCPDCRRFITKQLFPTWTMLQDIMSLTLVPYGNAKEVLSVNSPFSCQHGEPECRGNMIQVWKKIHALNWLMFNFCFMSGSLMLTVHLLKCLQLYAPSVSWAAVDACVTGRLGYKLMHGYAVMTRALNPAHTHVPWVTIDGEYTKEIEAKTSSLFHLICDRYKVRTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LC98</accession>
    <name>A0A3B5LC98_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RAB6A, member RAS oncogene family</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027886651.1">
        <property type="nucleotide sequence ID" value="XM_028030850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LC98"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008155"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008253.1">
        <property type="protein sequence ID" value="ENSXCOP00000008155.1"/>
        <property type="gene ID" value="ENSXCOG00000006259.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114152810"/>
    <dbReference type="KEGG" id="xco:114152810"/>
    <dbReference type="GeneTree" id="ENSGT00940000154769"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01861">
        <property type="entry name" value="Rab6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000139">
        <property type="entry name" value="ras-related protein Rab-6A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="23501" checksum="59408D0DA06F9517" modified="2018-12-05" version="1">MSAAGDFGNPLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTIRLQLWDTAGQERFRSLIPSYIRDSAAAVVVYDITNVNSFQQTTKWIDDVRTERGSDVIIMLVGNKTDLADKRQVSIEEGERKAKELNVMFIETSAKAGYNVKQLFRRVAAALPGMDTTQDKNREDMIDIKLEKPPEQPVSEGGCSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCV8</accession>
    <name>A0A3B5LCV8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Fucolectin tachylectin-4 pentraxin-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a defensive agent. Recognizes blood group fucosylated oligosaccharides including A, B, H and Lewis B-type antigens. Does not recognize Lewis A antigen and has low affinity for monovalent haptens.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fucolectin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCV8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008788.1">
        <property type="protein sequence ID" value="ENSXCOP00000008682.1"/>
        <property type="gene ID" value="ENSXCOG00000006037.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01060000248575"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042806">
        <property type="term" value="F:fucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010185">
        <property type="term" value="P:regulation of cellular defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001868">
        <property type="term" value="P:regulation of complement activation, lectin pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051941">
        <property type="entry name" value="BG_Antigen-Binding_Lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006585">
        <property type="entry name" value="FTP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45713:SF6">
        <property type="entry name" value="F5_8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45713">
        <property type="entry name" value="FTP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22633">
        <property type="entry name" value="F5_F8_type_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00607">
        <property type="entry name" value="FTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017406506" description="Fucolectin tachylectin-4 pentraxin-1 domain-containing protein" evidence="8">
        <location>
            <begin position="20"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Fucolectin tachylectin-4 pentraxin-1" evidence="9">
        <location>
            <begin position="22"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Fucolectin tachylectin-4 pentraxin-1" evidence="9">
        <location>
            <begin position="171"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="313" mass="34231" checksum="E4FFD0CE81230594" modified="2018-12-05" version="1">TMRHTSVLLLLLLLVTALAYEYKNVALRGKATQSHRLQSEFSVDAYNAIDGNRNSDLRRGSCTHTGPQAHPWWRVDLLESYVVTSISVTNRGDCCSENINGAGIHVGNSLQDDGTSNPVVAVISGIPPGRTLRITFTSPVEGRYVIVSLPGFNMVLTLCEVEIYGYRAPTGENIAIYGKATQSSLYTHGIAYNSIDGNRAGHWGKASCSHTIREFNPWWRLDLGKTHKVFSVSVTSSVEAYLRLQGAEIRIGDSLNNNGNNNPRCAAIGVISPGFTQNLQCNGMNGQYINIVIPGRTENLILCEVEVYASRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LD65</accession>
    <name>A0A3B5LD65_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">alcohol dehydrogenase (NADP(+))</fullName>
            <ecNumber evidence="9">1.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">S-nitroso-CoA reductase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosaccharides and bile acids. Acts as an aldehyde-detoxification enzyme. Also acts as an inhibitor of protein S-nitrosylation by mediating degradation of S-nitroso-coenzyme A (S-nitroso-CoA), a cofactor required to S-nitrosylate proteins. Also acts as a S-nitroso-glutathione reductase by catalyzing the NADPH-dependent reduction of S-nitrosoglutathione. Displays no reductase activity towards retinoids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>S-nitroso-CoA + NADPH + H(+) = sulfinamide-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:78375"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:145546"/>
            <dbReference type="ChEBI" id="CHEBI:145548"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:78376"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>S-nitrosoglutathione + NADPH + H(+) = S-(hydroxysulfenamide)glutathione + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:63500"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:145544"/>
            <dbReference type="ChEBI" id="CHEBI:229723"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a primary alcohol + NADP(+) = an aldehyde + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15937"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.2" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LD65"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007641"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007737.1">
        <property type="protein sequence ID" value="ENSXCOP00000007641.1"/>
        <property type="gene ID" value="ENSXCOG00000005878.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156539"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008106">
        <property type="term" value="F:alcohol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046185">
        <property type="term" value="P:aldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042593">
        <property type="term" value="P:glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19106">
        <property type="entry name" value="AKR_AKR1A1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000006">
        <property type="entry name" value="Aldo-keto reductase family 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044481">
        <property type="entry name" value="AKR1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="18">
        <location>
            <begin position="16"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="17">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="323" mass="36811" checksum="0958B4694AF25E4D" modified="2018-12-05" version="1">MNDFAVLNTGRKMPLIGLGTWKSEPGKVKQAVLWALEAGYRHIDCAAIYGNEVEIGEALQEILGPDKVKREDVFVTSKLWNNRHHPEDVEPSLLKTLKDLKLEYLDLYLIHWPYAFQRGDAPFPRKEDGSLLYDDTDYKVTWAAMEKLVEKGLVRSIGLSNFNSRQIDDILSVASIKPTVLQVESHPYLAQVDLLAHCRDRGLVVTAYSPLGSPDRAWKHPDEPVLLQEPAVLSLAEKYNKSPAQIILRWQTQRGVVTIPKSVTESRIKENIQVFDFTLEAEEMKSITALNRGWRYIVPMIEVEGKRVPRDAGHPHYPFNDPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDC5</accession>
    <name>A0A3B5LDC5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDC5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007696"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007793.1">
        <property type="protein sequence ID" value="ENSXCOP00000007696.1"/>
        <property type="gene ID" value="ENSXCOG00000005918.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155672"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="6">
        <location>
            <begin position="100"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="31"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="474"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="474"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="550" mass="60658" checksum="94ADAE1D037B2B22" modified="2018-12-05" version="1">MENALLMRVSVFTDQGGRKYMEDVTEVIVEPEPGEDEPTPGEQEAGNGGAFSVTAVDSHPDIKDGKAKTEQLTELSSERVRTAEKHIFEETISPGSECPPRDQASTSGRSRRSVAFFAVFDGHGGSEAAQFARDCLWEYIKKQRGFWSNCDREVCSAIRKGFVACHHAMWKKLPEWPKTLTGLPSTSGTTASVVVLRGNRMYVAHVGDSAIVLGVQDDPSVPFIKAIEVTQDHKPELPKERERIEGLGGSVIKKSGVNRVVWKRPRLSHNGPVRRSTIIDQIPFLAVARALGDLWSYDFYSGEFVVSPEPDTSVVILDPRKHRYIVLGSDGLWNMVPPQEAISMCQKNDEEMVPCGVTSARQLVSHSLLRWRQRMLRADNTSAIVIALLEPGGSKETFHNEEVLLDLAKVSQCPPTSISRADTPLLPQPPDEDSTSAMSELFPALERQDGLSGSNSFYHMTLSDPFSLSPLCSNSTMDSPSHSSLADRTVTRKTSTKRTLNGSPSSGLLAKKAKRRTDRPPLVQHNTEEKQKESVSPILQQHNKTTVCVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEU3</accession>
    <name>A0A3B5LEU3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LEU3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008304.1">
        <property type="protein sequence ID" value="ENSXCOP00000008206.1"/>
        <property type="gene ID" value="ENSXCOG00000006303.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157202"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000121">
        <property type="entry name" value="zinc finger protein 385B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051845">
        <property type="entry name" value="Znf385"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23067">
        <property type="entry name" value="DOUBLE-STRANDED RNA-BINDING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23067:SF6">
        <property type="entry name" value="ZINC FINGER PROTEIN 385C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="220"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="272"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="305"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="454" mass="49839" checksum="6724A41E233A8180" modified="2018-12-05" version="1">NENERVRRVRVGFKVKRERRQGSSSATMCQVCNIRLNSSAQAQIHYRGKTHQRRLRALSQNQVHPLLGSLPLPGRPLQPQTHARLEHFLPLRVNGSSPLSLFPNFNTMDPVQKAVINHTFGVSQPKKKPIISCNICHLRFNSTTQAEAHYKGHKHARKLKAMETQKNRQKNGPGQPSAGKDRVAETAMMEGETVQIDAELKDKTGTSSFFYLKTASPLQHPLGSASDPDPQGSAAAGGEEAAEGKEAKEAKTNKKPLHCLTCKVTVNSNSQLESHCSGSKHKQMLDGQNNSQSHRRVKVTSTPRPKSRMKQRMGSKSRTVVGVTNQAFHCELCQVSVNSETQLKQHTNSRRHKERLAGKHVKAKFTPYNKLHPNAVLATKLALHKQLSKALPAGLLSGPFNPAALCSMTSGPLALRLPPGPAAIIPGPIINPTLFRPAPGPLRVTHAPIIFSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFG1</accession>
    <name>A0A3B5LFG1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform</fullName>
            <ecNumber evidence="6">2.7.1.153</ecNumber>
            <ecNumber evidence="7">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Serine/threonine protein kinase PIK3CB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">PIK3CB</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phospho-1D-myo-inositol 4,5-bisphosphate + ATP = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phospho-(1D-myo-inositol 3,4,5-triphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43396"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:77137"/>
            <dbReference type="ChEBI" id="CHEBI:83243"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:43397"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.153"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:21293"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Heterodimer of a catalytic subunit PIK3CB and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interaction with PIK3R2 is required for nuclear localization and nuclear export. Part of a complex with PIK3R1 and PTEN. Binding to PTEN may antagonize the lipid kinase activity under normal growth conditions. Part of a complex involved in autophagosome formation composed of PIK3C3 and PIK3R4. Interacts with BECN1, ATG14 and RAB5A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.153" evidence="6"/>
    <dbReference type="EC" id="2.7.11.1" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFG1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006609"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006690.1">
        <property type="protein sequence ID" value="ENSXCOP00000006609.1"/>
        <property type="gene ID" value="ENSXCOG00000005115.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157522"/>
    <dbReference type="UniPathway" id="UPA00220"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046934">
        <property type="term" value="F:1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035005">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08693">
        <property type="entry name" value="C2_PI3K_class_I_beta_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00872">
        <property type="entry name" value="PI3Ka_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000001">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000046">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.70:FF:000004">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000005">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003113">
        <property type="entry name" value="PI3K_ABD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000341">
        <property type="entry name" value="PI3K_Ras-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF33">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE 3-KINASE CATALYTIC SUBUNIT BETA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02192">
        <property type="entry name" value="PI3K_p85B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00794">
        <property type="entry name" value="PI3K_rbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00142">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00143">
        <property type="entry name" value="PI3K_p85B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00144">
        <property type="entry name" value="PI3K_rbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51547">
        <property type="entry name" value="C2_PI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51544">
        <property type="entry name" value="PI3K_ABD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51546">
        <property type="entry name" value="PI3K_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0072">Autophagy</keyword>
    <keyword evidence="15" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K-ABD" evidence="27">
        <location>
            <begin position="22"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K-RBD" evidence="29">
        <location>
            <begin position="193"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="C2 PI3K-type" evidence="30">
        <location>
            <begin position="329"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="28">
        <location>
            <begin position="527"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="26">
        <location>
            <begin position="781"/>
            <end position="1062"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00065016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00068828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00076131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00080896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1079" mass="122983" checksum="8A91EA246AD3A547" modified="2018-12-05" version="1">MPPAMTDLLDIWAAHSPLAGHAPDQITVDFLLPTGIYIQMDVPREATIQHIKLLLWKQAQNFPLFPSLGEMESHMFECVNQAAVHEELEDETRRLCDVRPFLPVLKLVTRNCGRAERLLDSKIGVLIGKGLHELDGINDQEVKDFRSKMFRLSEEKMQRVQMMTCNEWLQACFSPQLEPAALTTITDGLNDRPADLKVIIHFDQSQDSTSLKVLSSSTPSELMEMAVKKWLITHAPEEEEWSGQYVLRVSHCLEFLCGDHPLIQYKYIRTCLQAKEPPHLTLVHTSTIKELFDKEMSAISAVVSRKSSNPPLPLPPKRRIPTVSPVQLWTIVLSFQIVLVKGSKVNAEESAKVQVRAGLFHGTELLCKPAVSSETSGRSEHTWRDNALEFDISISDLPRMTRLCFAIYAVMDKVKKQKSTKNPHINKYQTIRKAGKVHYPIAWVNTMVFDYKGQLKTGDIILHCWSSFPDELEEMLNPIGTIQTNPYTENATALHIHFPEYSPYPIVFPPFDKILEKAAQIARASDSMSIGRGGKKFHIELKEIMERDPLSQLCENEKDLIWTLRHDCRENFPQSLPKLLLSVKWSKHEDMAQLQALLQIWPKLCPRDALELLDFNYPDQYVREYAVGCLRDMSDEELSQYLLQLVQVLRYEPYYDCALTHFLLERAERNRKIGHFLFWHLRSEIHMPAVSVQFSLILEAYCRGSIPHIEVLKKQVEALTKLKSVNELIKLGTLKNARSKTKEAMLTKEAMITCLKQSGYSEALSDLSSPLNPSVLLSDISVEKCRYMDSKMKPLWIVYNNKLLNGDTLGIIFKNGDDLRQDMLTLQILRLMDLLWKEANLDLRIVPYGCLATGDRAGLIEVVSSADTIANIQLTNSNVAATAAFNKDALLNWLKERNSGDALDRAIEEFTLSCAGYCVATYVLGIGDRHSDNIMVRSTGQLFHIDFGHILGNFKSKFGIKRERVPFILTHDFIHVIQQGKTGNTEKFGSFRQYCEDAYLILRKNGNLFITLFALMLTAGLPELTSVKDIQYLKDSLALGKTDDEALKQFRQKFDEALRESWTTKVNWMAHNVAKDNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGV4</accession>
    <name>A0A3B5LGV4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Si:ch211-1e14.1</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000010087"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010206.1">
        <property type="protein sequence ID" value="ENSXCOP00000010087.1"/>
        <property type="gene ID" value="ENSXCOG00000007649.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063472"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024606">
        <property type="entry name" value="KIAA1549"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21590">
        <property type="entry name" value="SEA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21590:SF4">
        <property type="entry name" value="UPF0606 PROTEIN KIAA1549"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12877">
        <property type="entry name" value="KIAA1549"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="589"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="886"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="961"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1185"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1248"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1449"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="992"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1013"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1023"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1202"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1251"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1276"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1314"/>
            <end position="1335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1513" mass="165339" checksum="9E5017A1B98187C3" modified="2018-12-05" version="1">MSQQYQDKLIHVFVSSPIDYCNSVFTGLTKKSVLQLQLIQNAAAGILTKTRKIQHITQFYNPSKERVYVKTLLLVYKSLNGLAPQYIKDLLLLYQLSRSLRSSGSGSALHPQKQTWRSSIQLYSVVTSVANPPIEPPELALSESESQKVASSRQIVQNKSERNMNTLPSLDEIMAPGYHVPFITPQSSLIFSDPNQVPSEDFYPTNTMNVDWGSGDYLETMSFFNTDNDDYSLVTRVLSDPYDMEDDTEMYDTSFPSRVGISPSSRHPGYVSSSSSLMTPHLTVPPHQSINPSSLSTKVSNLLQPTDVAGTDAPDVSDIDWGDAFTIRPTDVLLPDMNSLEYYTTQLTKDSNASETGAEYRQNVTEISLNATVAPPSTDVITDGNLTEGESSSDLSGFGPHYESTTIVTTEITPQLSNASESFLDPSIIPTYILDPSSSVWGGPVSTTGWFTHTLTIDIDSALAEVLHPSATSLLPEDLASSSSLTDVHWCVTESFDDDSFATEQTGDMTLVSNESIVSITTTTSKITTAPSTTSRQYLCNVDHPAYLTKTGFPSGVTVGFAKSQVRDILKVEFNKSVELQVVSPPPQFVFRVVSGPVVYTAISVINALRRSARRFLYVSPNQTTPDQKYQVHTVLQFIPGHVDMRYCSFFESVEKGLIMAFAEVRRRSKESTNFTIHILNITTATNRYGEKQLTRQPVDITFTVSGSRGYLMGSKVSNALMKLTMVEFSYYIGFPVLQIAEPFHYPELNTSQLLRSSWVRTVLLGVLDKKMSERTFQANMERRVALLLGEAMGQVRRVKRATTVGNSSVQVAVSRLEGADNPAEMVYFVEGHNGQRMPAVQTANLLNSLDVQRAAIILGYRVQGVLAQPVEKLASSPSNSENNNMWIVIGVVVPLLVVVIIISILYWKLCRTDKLEFQPDAMTTIQQRQKLQAPSVKGFDFAKLHLGQQSKDDVMVIQEPALPGSGSAPLAQSIKDGFSPSENGEVPTPKSKTSASSTKASRGGRRRERISPSDGDSVVSDHSSEREPTEENPRAHATPSDSKQTRKAPINVLNGPPPMNGAANEQLSSASIFEHVDRMSRATDASRRLPNKVQLIAMQPMPVPPLHSQPVNGKPSDPNQMNKEIQAALRHKSEIEHHRNKIRLRAKRKGHYDFPAMDDMIGSLSDTKEQDRIYQKAQTQIDKILDPDNPVPAVFMDSKKSGRGRRSPKQRLKDQLNGGLIEADKDHLITEDSDAVYRKCPGVNNVAYVSDPDQGPSSPQRSPSPTDDVFLGPASSPPGHAPPPPPYLPPQPSIEEARQQMHSLLDDAFALVSPTSQSSTAGITLPGVNSNPQGCSPPGRGPRAWGPSYQPPFPGRFTELGVSSPAIQGLTPRQGLGSSYLPPGEAAGLSESLQPDSLFSSRGLYAKELPSSARPRPVGGTTGAQLHHLTQVGLSSRMNGYPAGIRMHRSRSGDPTAPPAHLDTSAAVAYLSSPPPLDTSPPTHSSASLIKAIREELLRLSQKQAAVSSYHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGV9</accession>
    <name>A0A3B5LGV9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. May also be required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Mitochondrion inner membrane</location>
            <topology evidence="1 14">Peripheral membrane protein</topology>
            <orientation evidence="1 14">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027891170.1">
        <property type="nucleotide sequence ID" value="XM_028035369.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027891171.1">
        <property type="nucleotide sequence ID" value="XM_028035370.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027891172.1">
        <property type="nucleotide sequence ID" value="XM_028035371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGV9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007104"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007194.1">
        <property type="protein sequence ID" value="ENSXCOP00000007104.1"/>
        <property type="gene ID" value="ENSXCOG00000005494.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114155465"/>
    <dbReference type="KEGG" id="xco:114155465"/>
    <dbReference type="CTD" id="26520"/>
    <dbReference type="GeneTree" id="ENSGT00940000160102"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042719">
        <property type="term" value="C:mitochondrial intermembrane space chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000004">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit Tim9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050673">
        <property type="entry name" value="Mito_inner_translocase_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13172">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 14" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="15">
        <location>
            <begin position="9"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="89" mass="10321" checksum="5B1C348D3DC85944" modified="2018-12-05" version="1">MAVQVTESDQIKQFKEFLGTYNKVTENCFMDCVKDFTTRDVKPEESSCSESCLQKYLKMTQRISMRFQEYHIQQNEALAAKAGLLGQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LH23</accession>
    <name>A0A3B5LH23_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LH23"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011433"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011565.1">
        <property type="protein sequence ID" value="ENSXCOP00000011433.1"/>
        <property type="gene ID" value="ENSXCOG00000008634.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164907"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09386">
        <property type="entry name" value="LIM1_LMO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000015">
        <property type="entry name" value="LIM domain only 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050945">
        <property type="entry name" value="LMO_RBTN_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45787">
        <property type="entry name" value="LD11652P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45787:SF8">
        <property type="entry name" value="LIM DOMAIN ONLY 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="6">
        <location>
            <begin position="14"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="135" mass="14962" checksum="2A940205A4414A1F" modified="2018-12-05" version="1">MVNSQASSVPPAPRSCAGCGGKIADRFLLFSMERYWHTRCLKCSCCQAQLGDIGTTCYSKGGMILCRSDYIKNRLIPGDRFHYINGTIFCEHDRPGAALHNSHLAPLQSSSVLTDQKVNERVTEMILQSHAAREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHX3</accession>
    <name>A0A3B5LHX3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">FAM20B glycosaminoglycan xylosylkinase</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM20 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027884175.1">
        <property type="nucleotide sequence ID" value="XM_028028374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHX3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011728"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011862.1">
        <property type="protein sequence ID" value="ENSXCOP00000011728.1"/>
        <property type="gene ID" value="ENSXCOG00000008856.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114151270"/>
    <dbReference type="KEGG" id="xco:114151270"/>
    <dbReference type="CTD" id="9917"/>
    <dbReference type="GeneTree" id="ENSGT00950000182951"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048701">
        <property type="term" value="P:embryonic cranial skeleton morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030166">
        <property type="term" value="P:proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd10470">
        <property type="entry name" value="FAM20B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024869">
        <property type="entry name" value="FAM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009581">
        <property type="entry name" value="FAM20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450">
        <property type="entry name" value="DENTIN MATRIX PROTEIN 4 PROTEIN FAM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450:SF14">
        <property type="entry name" value="GLYCOSAMINOGLYCAN XYLOSYLKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06702">
        <property type="entry name" value="Fam20C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017449655" evidence="9">
        <location>
            <begin position="23"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="FAM20 C-terminal" evidence="10">
        <location>
            <begin position="190"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="222"/>
            <end position="225"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <location>
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        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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        <location>
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        <ligand>
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        </ligand>
    </feature>
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        </location>
        <ligand>
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        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00006557"/>
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            <dbReference type="ARBA" id="ARBA00023034"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
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        <source>
            <dbReference type="Pfam" id="PF06702"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="409" mass="45971" checksum="F726B332575C4006" modified="2018-12-05" version="1">MKLKQRMVVLCAVLLLLGLAKIFLLDGGEGSAASRRDLRAFRKMESGLSLPRGAHLTHTLQSPWEIASQWVGPREVYPEETPELAAVLTSLSTARIERADVGYKGTQLKALLVLDGGQKVVFKPKRYKRDYVVEGEPYAGYDRHNAEVAAFHLDRILGFRRAPLVVGRYVNLRTEIKPVATDQLLSTFLMQGNNTCFYGKCYYCRETEPACADGETMEGSLTLWLPDVWPLQKHRHPWGRTYREGKLARWEYDESYCEAVKKMPPYDAGPRLLDVIDTAVFDYLIGNADRHHYESFQNDGGASMLILLDNAKSFGNPSLDERSILAPLYQCCMIRVSTWNRLSFLKGGVLSSAMRQALAFDPINPVLAEPHLAALDRRLNGVIATVKQCMEAQGPDNALIEDRMNLPHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIA9</accession>
    <name>A0A3B5LIA9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
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            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="component" value="Unplaced"/>
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        <property type="evidence" value="ECO:0007669"/>
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            <begin position="1"/>
            <end position="17"/>
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            <begin position="18"/>
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    <sequence length="104" mass="11841" checksum="10E7B5442A9EAC0D" modified="2018-12-05" version="1">MLLWRIVMRLSVGGVLVQRDLTPVITPVIPVWGVGLLKNDDDDEVSNKTWVLTPKVYESDVTHILNSLLDGYDNKLRPDIGGMDNSSVRSINQHIKEKWLKNFI</sequence>
</entry>
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    <accession>A0A3B5LLB7</accession>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <authorList>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="62"/>
            <end position="94"/>
        </location>
    </feature>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="97" mass="10889" checksum="B376709FC1B51DC5" modified="2018-12-05" version="1">MAACENNHLDTVKYLVRAGATVGHKDIMGFTCLHLAAKLGHYDVVDHLLNKASKYINCQDNGGWTAITWAIEHKHKELVHLFLRKGADVNIRDKVSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LN46</accession>
    <name>A0A3B5LN46_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pleckstrin homology domain-containing family J member 1</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="GOC"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045188">
        <property type="entry name" value="Boi1/Boi2-like"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22902:SF9">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY J MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22902">
        <property type="entry name" value="SESQUIPEDALIAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="15"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00041004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="158" mass="18612" checksum="B1F7985048E0F405" modified="2018-12-05" version="1">MRFNEKELVCLSRQPSEMAAELGMRGPKKGDVVKRRLVKLVVNFLFYFRTDEEEPIGALLLEQCHVEREDSQTFSIAFLDEAERKYLFECDSEEQCKEWMDSIIKASYEFMRKNLIFYRTEIHRLTGKDPLEQYGISDETRFQVNSGSQLMTRDTSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNA9</accession>
    <name>A0A3B5LNA9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family. Snake venom subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNA9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012487"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012638.1">
        <property type="protein sequence ID" value="ENSXCOP00000012487.1"/>
        <property type="gene ID" value="ENSXCOG00000009437.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163484"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000010">
        <property type="entry name" value="Kallikrein related peptidase 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF52">
        <property type="entry name" value="GRANZYME K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="6">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="239" mass="26731" checksum="DFF2A1B252E8501F" modified="2018-12-05" version="1">ISLSINIHVSTYVLITHIILSHLQTNPQRGSETDHIKFNHAVLLFSPRIQTVLLGVHSIKEKKNEQQYRQVRKVKRSVPHPCYDSKEKNNDLMLLKLDKAVKQTKWVKTLKLNKAVKEPAAGSVCKVAGWGKTKNDATTMSDVLMSTNVTVIDRMKCNSKDYYNLAPYITKNMICAGWNGKIKADTCQGDSGGPILCNEDLVGVTSFGHGCGIQNKPGVYAFLTEDNLNWVKKTMKTNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNJ6</accession>
    <name>A0A3B5LNJ6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNJ6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011021"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011148.1">
        <property type="protein sequence ID" value="ENSXCOP00000011021.1"/>
        <property type="gene ID" value="ENSXCOG00000008326.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234818"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="56" mass="6612" checksum="E5905D3192DA4379" modified="2018-12-05" version="1">VELLPAIVMENDEDDDGNFTNWMSSYWGHGTDRGHSRERKRSFRRPSKTNLDQKQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNL5</accession>
    <name>A0A3B5LNL5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Decapping nuclease</fullName>
            <ecNumber evidence="5">3.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 5'-end CoA-ribonucleoside in mRNA + H2O = 3'-dephospho-CoA + a 5'-end phospho-ribonucleoside in mRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67496"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17276"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:172371"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:67497"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 5'-end FAD-phospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-ribonucleoside in mRNA + FAD + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67492"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17275"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:172372"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:67493"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 5'-end NAD(+)-phospho-ribonucleoside in snoRNA + H2O = a 5'-end phospho-ribonucleoside in snoRNA + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60892"/>
            <dbReference type="Rhea" id="RHEA-COMP:15699"/>
            <dbReference type="Rhea" id="RHEA-COMP:15700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:144029"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:60893"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Binds 2 magnesium ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the DXO/Dom3Z family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNL5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013683"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013851.1">
        <property type="protein sequence ID" value="ENSXCOP00000013683.1"/>
        <property type="gene ID" value="ENSXCOG00000010357.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006425"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034353">
        <property type="term" value="F:mRNA 5'-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110155">
        <property type="term" value="P:NAD-cap decapping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013961">
        <property type="entry name" value="RAI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039039">
        <property type="entry name" value="RAI1-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12395:SF9">
        <property type="entry name" value="DECAPPING AND EXORIBONUCLEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12395">
        <property type="entry name" value="DOM-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08652">
        <property type="entry name" value="RAI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RAI1-like" evidence="7">
        <location>
            <begin position="56"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="258" mass="30399" checksum="78C75CE043F167B5" modified="2018-12-05" version="1">KRGGDNGRRNHCDGAQFRPSNHHLHQSSSTPRQTPPRSQTLSTRREVYERDFPLYKQPVEVGCFSLDSQRRFFNDSRQMRYYVEPERSPSFDLRDGYRERYVKRDENVKEKLDHILRWIVANRSKLKSNPLDFDFVTWRGHLTKLLTTPYETREGWLLAVTRFNGTLYISEVETEAACRERENRPERNKEMMYWGYKFEQYMCAGEGTPDPSGVVNTNEAFCTVVQTRLTDHNQTERSGGELKAYTSTHVLQESQKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPK4</accession>
    <name>A0A3B5LPK4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GST N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPK4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014033"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014208.1">
        <property type="protein sequence ID" value="ENSXCOP00000014033.1"/>
        <property type="gene ID" value="ENSXCOG00000010623.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000049601"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016034">
        <property type="term" value="F:maleylacetoacetate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000221">
        <property type="entry name" value="Glutathione S-transferase rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42673">
        <property type="entry name" value="MALEYLACETOACETATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42673:SF4">
        <property type="entry name" value="MALEYLACETOACETATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="181" mass="20381" checksum="EDC1EE25EE11D6E1" modified="2018-12-05" version="1">MSLTASGLCSSVCKAGHGAPPEKKLQGYKQKLLSFEKEEHKSKEVLEINPRGQLPAFKHGDNIINESFGACLYLENQFKSQGNKLIPDNPAEQALMYQREQSIAFFFFFFLQNVFNTSFLVKASVGAPSCPNTSSLNHLEDKDFWDGNLCPTNLGQKDNPHFVRGCLCNYNPHKLHLFLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQC1</accession>
    <name>A0A3B5LQC1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">O-acyltransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(11Z)-octadecenoyl-CoA + cholesterol = cholesteryl (11Z)-octadecenoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:64324"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:75121"/>
            <dbReference type="ChEBI" id="CHEBI:88768"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:64325"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(7Z)-octadecenoyl-CoA + cholesterol = cholesteryl (7Z)-octadecenoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:64328"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:152049"/>
            <dbReference type="ChEBI" id="CHEBI:152050"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:64329"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(9Z)-hexadecenoyl-CoA + cholesterol = cholesteryl (9Z)-hexadecenoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:64320"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:61540"/>
            <dbReference type="ChEBI" id="CHEBI:84323"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:64321"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(9Z,12Z)-octadecadienoyl-CoA + cholesterol = cholesteryl (9Z,12Z)-octadecadienoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:42796"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:41509"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57383"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:42797"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>cholesterol + hexadecanoyl-CoA = cholesteryl hexadecanoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:42792"/>
            <dbReference type="ChEBI" id="CHEBI:3663"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:42793"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>octadecanoyl-CoA + cholesterol = cholesteryl octadecanoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:42812"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:82750"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:42813"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Endoplasmic reticulum membrane</location>
            <topology evidence="1 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQC1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014308"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014486.1">
        <property type="protein sequence ID" value="ENSXCOP00000014308.1"/>
        <property type="gene ID" value="ENSXCOG00000010845.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183081"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034736">
        <property type="term" value="F:cholesterol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033344">
        <property type="term" value="P:cholesterol efflux"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF6">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000439">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 17" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 17 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="175"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="314"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="420"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="active site" evidence="18">
        <location>
            <position position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000439-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="493" mass="58218" checksum="8563FD403B96B72F" modified="2018-12-05" version="1">KIEVEHVISKKLQLKRKAEGLKSDLMRQFESQVHDFMDSLIEESASLESTPLPAAFSPPQSDKERSKLHVQPPQGHGKHFVTQRSLLELFEVNHIRTIYHMFIALLILFILSTFVVDFLDEGLVLDFDLLVYAFGQFPLVVCTWLCMFLSVLLVPYTLFHLWSQSQSGSHGQQRLYTWLFGSAFLLYQGLCLGFLPTYVVVTNSLPPASCFIVIIEQVRLMMKAHSFVRENVPRVLAWQKDKTPQVSQYLYFLFAPTLIYRDKYPRNPVTRWGYVAAKLLQVLGCLFYTYYIFVRLCIPQFRSISLQLFNLRAMVLCVFNSILPVLVLLLGFFAFLHCWLNAFAEMLRFADRMFYKDWWNSTSYANYYRTWNVVVHDWLYYYVYKDFLLSQKRFRPAAMFFVFTVSAVVHEYILAVSFGFFYPVLFCLFMCFGMFNFIFHDRRKGPIWNILMWTSLFLGQGVIMCLYSQEWYARRYCPLKESSFLDLLKPRSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQS2</accession>
    <name>A0A3B5LQS2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Anaphase-promoting complex subunit CDC26</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cell division cycle protein 26 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CDC26 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027861229.1">
        <property type="nucleotide sequence ID" value="XM_028005428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQS2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011751"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011886.1">
        <property type="protein sequence ID" value="ENSXCOP00000011751.1"/>
        <property type="gene ID" value="ENSXCOG00000008882.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114136975"/>
    <dbReference type="KEGG" id="xco:114136975"/>
    <dbReference type="CTD" id="246184"/>
    <dbReference type="GeneTree" id="ENSGT00390000008457"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018860">
        <property type="entry name" value="APC_suCDC26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28579">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT CDC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28579:SF1">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT CDC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10471">
        <property type="entry name" value="ANAPC_CDC26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="94" mass="10065" checksum="51B04F359B467FD5" modified="2018-12-05" version="1">MLRRKPTRLELKIDDTEEFESVKKELEARKRQREEAGPGAGVGGASVVGVDMIGGGASASSTAASRAELINERIGYKPHPKPATLPTLFGSLQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTH9</accession>
    <name>A0A3B5LTH9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Neurexin 3b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTH9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011119"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011246.1">
        <property type="protein sequence ID" value="ENSXCOP00000011119.1"/>
        <property type="gene ID" value="ENSXCOG00000008403.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Laminin"/>
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    </dbReference>
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        <property type="entry name" value="EGF"/>
    </dbReference>
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        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
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        <property type="entry name" value="AXOTACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
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    </dbReference>
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        <property type="entry name" value="EGF_3"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017442676" description="Neurexin 3b" evidence="3">
        <location>
            <begin position="24"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="4">
        <location>
            <begin position="23"/>
            <end position="203"/>
        </location>
    </feature>
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        <location>
            <begin position="194"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="27053" checksum="2CDBAE2CD4573634" modified="2018-12-05" version="1">MNPYSLPGHFCLLLSTLLSLSAGLEFTGSQSQWARYVRWDASSRGDLTFQFKTSQSDGLLLYFDDGGYCDFLLLALSEGKLQLHVSIDCAETTITSDKPVNDSRWHFAAINRENLWTGLAVDGQSKTGEVRPQRQFMKIVSDLFLGGLPRDIRTAAITLPSVRELPPFKGIITNLSYGSKLPTLVNSQKVRLEMMGLCTENPCENGGICSLADGEVYCDCSKTGYVGRYCTEGKRAGFVVFCVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LU01</accession>
    <name>A0A3B5LU01_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LU01"/>
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        <property type="protein sequence ID" value="ENSXCOP00000012796.1"/>
        <property type="gene ID" value="ENSXCOG00000009668.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286939"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zinc finger protein 143 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF44">
        <property type="entry name" value="ZINC FINGER PROTEIN 271-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="177"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="242" mass="28130" checksum="7AE1739D1220EF64" modified="2018-12-05" version="1">RTTSSATRRPNCRNRSPAISALKCFFLCLDAAEDLAAPKIPELENSESQKTQEAALKPKGKEFVCELCKKRFSRRFHLAKHMNTHKEQRPFACDRCPRKFRTAETLEQHLLRHEEKKHASFQCQRCEKLFKTKMNLKTHQLVHSDLRPFSCGTCGKAFKTKHNLQRHIRIHTGEKPFACKVCGRPFMQDNKLKAHMLLHGAAKAFMCDLCGKTFLYNCQLKKHQKALCWCCRCPRARQRSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUN7</accession>
    <name>A0A3B5LUN7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Inhibitor of growth protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Component of an histone acetyltransferase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of an histone acetyltransferase complex. Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ING family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUN7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015708"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015902.1">
        <property type="protein sequence ID" value="ENSXCOP00000015708.1"/>
        <property type="gene ID" value="ENSXCOG00000011865.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156619"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16858">
        <property type="entry name" value="ING_ING3_Yng2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15585">
        <property type="entry name" value="PHD_ING3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000103">
        <property type="entry name" value="Inhibitor of growth protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042020">
        <property type="entry name" value="ING3_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024610">
        <property type="entry name" value="ING_N_histone-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF103">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12998">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01408">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="4 12 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 12 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="16">
        <location>
            <begin position="349"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="126"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="277"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="179"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="376"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="351"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="362"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="366"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="11">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="407" mass="45042" checksum="5E3AFBEC232D885B" modified="2018-12-05" version="1">MLYLEDYLEMIEQLPMDLRDRFTEMREMDLQVQNATDQLEQKVTEFFLNAKKNKPEWREEQMEIIKKDYYKALEDADEKVQLANQIYDLVDRHLRKLDQELAKFKMELEADNAGITEILERRSLEMDSPSQPVNNHHVHSHSSSESESSHPPKKFKSEALLSTLTSDASKENTPGCRANSTSSSTNSVYSVNPSQPLASYSLTSLPAGPGAGAGAITMAAAQAVQATAQMKEGRRTSSLKASYEAIKNNDFQLGREFSLTRENSGYSSSALASTLTQNLTPSAASDSRGRKSNSIKSSNHQSSSSSSSSSLSSCSSSSALAQELSQQAAVLPEADANSQVDWTYDPNEPRYCICNQVSYGEMVGCDNTDCPIEWFHYGCVGLTEAPKGKWFCPQCTAAMKRRGSRHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUU7</accession>
    <name>A0A3B5LUU7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUU7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011604"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011736.1">
        <property type="protein sequence ID" value="ENSXCOP00000011604.1"/>
        <property type="gene ID" value="ENSXCOG00000008758.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000271588"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055107">
        <property type="term" value="P:Golgi to secretory granule transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051149">
        <property type="entry name" value="Spindly/BICDR_Dynein_Adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123:SF13">
        <property type="entry name" value="BICAUDAL D-RELATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123">
        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="70" mass="8146" checksum="4F0BC52A1D81735E" modified="2018-12-05" version="1">MELAKCKIDIMSLNSQLLDSIQQKLNLSQQLEAWQVGQRGQEANKGAAFFKIENRNFVYVVQLNLFRQLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWD7</accession>
    <name>A0A3B5LWD7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">V-set and immunoglobulin domain containing 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">VSIG10</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWD7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013636"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013803.1">
        <property type="protein sequence ID" value="ENSXCOP00000013636.1"/>
        <property type="gene ID" value="ENSXCOG00000010328.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159876"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="entry name" value="V-SET AND IMMUNOGLOBULIN DOMAIN-CONTAINING PROTEIN 10"/>
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    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="2 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="30"/>
            <end position="108"/>
        </location>
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        <location>
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        <location>
            <begin position="225"/>
            <end position="300"/>
        </location>
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            <end position="396"/>
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        <location>
            <begin position="488"/>
            <end position="524"/>
        </location>
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            <end position="524"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="PROSITE" id="PS50835"/>
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            <dbReference type="Ensembl" id="ENSXCOP00000013636.1"/>
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            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="524" mass="57605" checksum="C511B1ED8C40C8DE" modified="2018-12-05" version="1">MRFNSFLLSRLKATVSGNVSTETVQSAAPGDVVLLQCYTGGNLTPVWTTWMKDGHVIASNGELHVDSSAKDPRLTLERNGSLSISKVISDDTGSFLCSSRLPDNRTINAHVLLQVTSGPNNVSTMIYPTIVLPNRTLVSKKGDSLCFKCMAWSSPSQQLNLTFSGASNNNTSLAFTPFPVLDYTIKDIQPSNQGVYTCSALNTVSNRMVKNSTELLVYYIPSGHPDCMSVETQNSSSVQLNCSWFGAYPTPELRWGKDGDFQVTDSLVVSLNRSTLQEGQTLTCTARHELLKIENERSCSITLRAPYPQGDPMVSVLEGTNVTLSCREISSVPAANTTWRKGQSLEPIVPGSKYILSVEGPDLKLTIVNISKYDEVYYFCRSENALGIRELEVFLTVKASSSTYTGAVIGVFIAALITGCAFIVAKILYSRRHTICLGNCSEDRGDVVDLVESDDEQIFQDAVPRLPPLTNGRHTTLVQIHRIPSSEDHEVIVETHPEPPEQKEEPTEELTEESREEVEELVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXQ8</accession>
    <name>A0A3B5LXQ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Short coiled-coil protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
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            <taxon>Xiphophorus</taxon>
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    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Positive regulator of amino acid starvation-induced autophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
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            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
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        <subcellularLocation>
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            <location evidence="4">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SCOC family.</text>
    </comment>
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        <property type="gene ID" value="ENSXCOG00000012024.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Short coiled-coil protein a"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR21614">
        <property type="entry name" value="SHORT COILED COIL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="18"/>
            <end position="52"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="9184" checksum="20A7CCF6BE7B0138" modified="2018-12-05" version="1">MNCDIDGDLENQVEMEEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LY66</accession>
    <name>A0A3B5LY66_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LY66"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014286"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014463.1">
        <property type="protein sequence ID" value="ENSXCOP00000014286.1"/>
        <property type="gene ID" value="ENSXCOG00000010829.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of osteoclast differentiation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="T178A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32005:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 178A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32005">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 178B-RELATED"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017365128" evidence="3">
        <location>
            <begin position="24"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="322" mass="36552" checksum="A94DD6A2D075D1C0" modified="2018-12-05" version="1">CSSFLPIALLALSSLSLLLLVTAISTDHWYQTDTRRHKQNCDGSDSNDQKNREMPIYHLPLVDTGGAAARQRDVALMKPVHVGSREEELLENWRAILGMGILETECGRPLFSTHSGLWRKCYFKGLDPDIDKLIDRGIADRCTAVKYHFSQPIRLRNIPLNLTRTIQQDEWHLLRKHTHTHTHAQIGSVNVGPQPGCKYCKCSDLVLFCSRVLIVLFIQLLSHKLEGTNRTEPVSRSESLWVRKNRTRYISLTTLVLPELWQNLLQNPGLDPAASQMELPQNPVYRSSSVTVEPGQELLDQNKMPQVHLSRPGFCPHCPETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LY80</accession>
    <name>A0A3B5LY80_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LY80"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014301"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014478.1">
        <property type="protein sequence ID" value="ENSXCOP00000014301.1"/>
        <property type="gene ID" value="ENSXCOG00000010843.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00910000144115"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000078">
        <property type="entry name" value="serine/arginine-rich splicing factor 7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050907">
        <property type="entry name" value="SRSF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23147">
        <property type="entry name" value="SERINE/ARGININE RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="11"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="5">
        <location>
            <begin position="105"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="78"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="89"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="147" mass="16553" checksum="5C81B3E91A4354A7" modified="2018-12-05" version="1">MSRFGRYGGESKVYVGNLGTGAGKAELERAFGYYGPLRTVWIARNPPGFAFVEFEDSRDAEDAVRGLDGKVICGSRVRVEPSNGMPRRSRYERPPPRRPFDPDDKCYECGQKGHYAYDCHRYGRRGHRSRYAAAAALRSRRPSALVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYW7</accession>
    <name>A0A3B5LYW7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">JunB proto-oncogene, AP-1 transcription factor subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">JUNB</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family. Jun subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYW7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016140"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016345.1">
        <property type="protein sequence ID" value="ENSXCOP00000016140.1"/>
        <property type="gene ID" value="ENSXCOG00000012198.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161195"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14696">
        <property type="entry name" value="bZIP_Jun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000012">
        <property type="entry name" value="Putative transcription factor AP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050946">
        <property type="entry name" value="AP-1_TF_bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005643">
        <property type="entry name" value="JNK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002112">
        <property type="entry name" value="Leuzip_Jun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11462:SF58">
        <property type="entry name" value="JUN B PROTO-ONCOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11462">
        <property type="entry name" value="JUN TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03957">
        <property type="entry name" value="Jun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00043">
        <property type="entry name" value="LEUZIPPRJUN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="251"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="249"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="330" mass="36411" checksum="A688A2B1ADA316BC" modified="2018-12-05" version="1">MSTIMEQPFYDDSFLSAYGHPGAALPDYKLLKQNMNLNFSDSYRNPSFKTQHLRADSDFYPAGTADVGSLKLASPELERLIIQSSNGVITTPTPAQYLYNRGITEDQEVFAEGFVKALDDLHKMNQMAPPNVSIGTGGLSCPAPGAVFAHSMQTEPLEYTTLSSCTTNPSLASTASYPSTTISYLPHHQYHQHPQAVAHGSHHFQHSLAGAGVHSQRFGGLKEEPQTVPDMQSSDNSSPPMSPIDLENQERIKAERKRLRNRLAASKCRRRKLERIARLEDKVKVLKTDNAGLSNTASLLREQVAQLKQKVMTHVSSGCQLMLAPKVKSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M122</accession>
    <name>A0A3B5M122_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Proton-coupled folate transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Solute carrier family 46 member 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate(in) + H(+)(in) = (6S)-5-methyl-5,6,7,8-tetrahydrofolate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:70167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>folate(in) + H(+)(in) = folate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:70159"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:62501"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>methotrexate(in) + H(+)(in) = methotrexate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:70163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:50681"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>pemetrexed(in) + H(+)(in) = pemetrexed(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:70171"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:63724"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Basolateral cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the major facilitator superfamily. SLC46A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M122"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016950"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017169.1">
        <property type="protein sequence ID" value="ENSXCOP00000016950.1"/>
        <property type="gene ID" value="ENSXCOG00000012779.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183096"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005542">
        <property type="term" value="F:folic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507:SF2">
        <property type="entry name" value="PROTON-COUPLED FOLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507">
        <property type="entry name" value="ZGC:174356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0290">Folate-binding</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0769">Symport</keyword>
    <keyword evidence="8 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="293"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="360"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="415"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00038227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00040650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="467" mass="50744" checksum="38AEBF28829FAF30" modified="2018-12-05" version="1">MDEPDTAAILPEDLLGDRKDDAGLPTATTSVPPSCRCPVPVSVEPVLFLSMFSLALQAPLTTQYLWDRISEDLGYNGSRRSGCSNSSLDPDPLQKEVETLTAHWNLYMSLGGFSVGLVVVPLLGSWSDVAGRRTVLILPNFGLALQAVVYLVVMYLKLPVVYFLVGRLLSGLSGDFNAVLAGCFSYVADISDRRSRTFRVAVLEACLGISGMLASIIGGQWRRAQGYINPFWLVLATNLASALYAYLFIRESVRPDPSARLLSARHYKAVWRLYSTGGISGEGGGALLHRGKLWLYTLCFFLVVAVHFGTRELYVLYELSSPLCWDSALIGYGSAAQNLAYLSSLLGLKAMQRCLQDSWVALVGLVSNITGLVVFSVADTTQLMFTGYCLCFLFMASTPVLRSKLSKLVDPSEQGALFACVACVESLCFLVGSSLFNSIYPATLHFMKGFSFLFAAIILLIPTAIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M1E4</accession>
    <name>A0A3B5M1E4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M1E4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017090"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017310.1">
        <property type="protein sequence ID" value="ENSXCOP00000017090.1"/>
        <property type="gene ID" value="ENSXCOG00000012886.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156462"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000003">
        <property type="entry name" value="Collagen type VI alpha 3 chain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF13">
        <property type="entry name" value="COLLAGEN ALPHA-3(VI) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="VWFA" evidence="8">
        <location>
            <begin position="5"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="8">
        <location>
            <begin position="205"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="8">
        <location>
            <begin position="406"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="8">
        <location>
            <begin position="590"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="767" mass="83370" checksum="0C6E0C72FF2276AC" modified="2018-12-05" version="1">PDSTDLIFLIDGSLNVGAANFPYVRDLVTRLVERLSVSRDGIRVALVQYNADPQIKFYLNSYYEKSSVLEAVKGLSFSGDDESNLGAALEEVADSLLGESAGGRAEEGAPQVLVVISAGASSDDTGAGHRAVIRAGVVTLGVAVGADAAAELEEVATDKSFVLKADSFRDLTAADDNLYGYINGLATRSIIFHNEFTEVTVGERDIIFLIDSTMGTAVINTVKEYIRRFVSTKEIGPNAVQVGIAQFATDTRLVMDLQTHATKESLLSSLATIRPRQGQTINIGAALDFVRLNMLRPEKGSRMGRGVPQLLLLIVSKRSSDSVAGPAQALENLRVLTLAAGSRAAAEAELKQIAFPESMVFISKDFRQLFRNAREITDALSTLSGRMVEPPTDPITTVQTQRVFRDIVFLVDGSNYVGSNNLPFVRDFMINIVNQLNISPEQVQIGLMQFAEEPRIEFYLNTYDNKQDVVEKISQLRLTGGSVLNTGAAMSYALDNMFQTSSGSRKLQGAVQVLILITGGPLQDDARNEADRLAVGSILTFTVSSGQADQEEMEKIAFIPTLAYHKQSFFDLPALADDIMPSLISVVERDVAFLIDGTDGVRGDFPHIRDFILKVIEPLDIGFNKVRVSVVQHSERPTPVFYLNTYQTKEEVIRAIQGMALAGGRSLNTGAALRYMKATVMSDSNGSRAAQNVPQFLIVLAADRSMDSVKEPAGELKTDGVVPFGVGVKNADKKQIEAIAHNPSFAFNVKEFRELEPILCSCWMGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2T0</accession>
    <name>A0A3B5M2T0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM229 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M2T0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018645.1">
        <property type="protein sequence ID" value="ENSXCOP00000018413.1"/>
        <property type="gene ID" value="ENSXCOG00000013864.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010899"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31746">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 229 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31746:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 229A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="359" mass="41767" checksum="549E11DFC8700232" modified="2018-12-05" version="1">MASRWREGHRSRSREHGISLETAAAPRQQEPSGDAGESLRELPRWMRLYLYGMHGVTLDVLLSSLQGVLTSRDPKLVGFSSPYLCIMHALTHFALEQIYSQKKCFRGRPVVFHLVFYPSVYIGLQILVGNINTLTEQVRVVSGTQLVVHYILALYFSQVFHRGLARLQYQPTSRCADLWRSLGGPDHHHHHHHHHHHHHHHHHHHPSGGLPGFARFLFFGMHGFLDEVLFTSLFNLVEKGVRATGGHTSLWSFLMYGSCSFVVEKLYFHLHFGRGWGTWRRLPIYICFIYTWEFCCGLTLRQFDACSWDYSHYPHNFMGLITLLYLPGWLILSLYQDVLINIFFLLYFYLPLNFPIMGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M396</accession>
    <name>A0A3B5M396_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DENN domain containing 4A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">DENND4A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSXCOT00000018852.1">
        <property type="protein sequence ID" value="ENSXCOP00000018618.1"/>
        <property type="gene ID" value="ENSXCOG00000014013.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155836"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032483">
        <property type="term" value="P:regulation of Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000042">
        <property type="entry name" value="C-myc promoter-binding protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.100.10.50:FF:000001">
        <property type="entry name" value="DENN domain containing 4C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051696">
        <property type="entry name" value="DENN_Domain_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023341">
        <property type="entry name" value="MABP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018798">
        <property type="entry name" value="MVB12A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296:SF16">
        <property type="entry name" value="C-MYC PROMOTER-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03455">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10240">
        <property type="entry name" value="DUF2464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13812">
        <property type="entry name" value="PPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00801">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00800">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51498">
        <property type="entry name" value="MABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MABP" evidence="6">
        <location>
            <begin position="41"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="UDENN" evidence="5">
        <location>
            <begin position="190"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="814"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="899"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1124"/>
            <end position="1168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1224"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="941"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="953"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1150"/>
            <end position="1165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1602" mass="178352" checksum="29C47CA199687B49" modified="2018-12-05" version="1">MEDKGPRVADYFVVAGLTDPSKPLDQEIHFDDVCHKTAKPKAPITDVAVVIRSLGEEVPPGFACIETTPSGHLADLNNGGLMAPQIFLCYRRGRDKPPLTDLGVLYEWKERLKQGCHLIQTTPAGRPANISGNSSQRIYVTYRQAPDSQPHASLAVTDICIIIPSKGETPPHTFCKVEKNLNSSMWGSSVYLCYKKSVAKTNVIAYKAGLFSRYPEEDYESFPLPESVPLFCLPMGATIESWPANTKYSLPVFSTFVLTGASGEKVYGAAIQFYEPYPQECLTDTQLSQLGLLSPEQCNASPSSSAADAAINPGNGAANTSSVYTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHALPIEKHISHFMHKVPFPSSQRPRILVQLSPHDSLMLSQPVSSPLPLSGGRFSTLLHNLGPENAVTLLVFAVTEHKILVHSLRPAVLTSVTEALVSMIFPFHWPCPYIPLCPLALADVLSAPCPFIVGVDSRYFDLYDPPPDVNSERERDFKTNFCYILGKACKNLMNVLSNLHQQLVDGQYRSDGLLELSMSDSQELSCGKSLHTLELEIQEAFLRFMAAILKGYRSFLRPITQAPSEKATDASSLFDLQGFLKSRDRSHQKFYSLMTKTQMFIRFIEECSFVSDKDTSLAFFDDCVDKVDGERPDETRLIEIDESQRSEHTVFITPPELPPLPDGEEYPLCYRYDGFPVLTLELFDPVEGLRTPSSRLAARHSCPTSPAPMFRRTKQEIKSAQKIAKKYSSIPQLWSKCLLRHCYGLWFICLPAYVKVCHSKVRALRTAYDVLRKMQAKKLQPPDEVCYRVLMQLCGQYGQPVLAVRVLFEMKKAGVHPNAITYGYYNKAVLESTWPSSTRGGYFLWMKLRNVILGVAQFKRALRRHGPLTQSPLSDGSDLDAVSHGSLDSSADPNPAEQSPVEPADDRSSTGDQSDLGYNSLSKEEVRRGDAAPPDKDEKKESDCSSCQYYSDEDKSNTEAIFDFEDLDLEKPALGVRHLKKPVERSASYGGMSSAVPRGAVTRTGIETGFDPLSLMAAESQIAAYPDGDEAGAPGARRNLAREIELYMSHLDTPLSSRTSSSELQGPASPLLLHPHAAAAFRRSSLPHAAPLRTSAMPRSRTFHQASPARPASRQRLVSSPSGRSSSTSPSLSPFRERLASPSSSAFALDALLTPTLDVFKTSVVSAGKGVAERASRWYSRFATSNTPTKDGFSDRLSVSSLNVGDPDGSIQDEGEYGETEQLGVSPQKNGPSCPHRSPVHSRVDSPTAGRSLGRPTSLPPGENFLYLRLDLLISSCSRCKTCDCLVYDEEIMAGWTADDSNLNTTCPFCGNPFLPFLNVEIRDMRGPGSMDWQLHNPEPVTVPYLSPLVLWKELESLLENEGDPVITEADMVDHHPIIYWNLLWYFRRLDLPSSLPGLILTSEHCNRDSQVPRHWMSEDSKHVLIQILWDNLKLHQDPVQPLYILWNTYNVGYPLSRPVPDEEKPLTDELLQGVVRSIQRNDVGRPMAQLLQLLDRMLGVKRQRSLYRDILFLSLVALGKDNIDIDAFDREYKLAYDRLLPPLVRLTHNCDRPPGAGVMECRRTFGEPYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3J6</accession>
    <name>A0A3B5M3J6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the anoctamin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3J6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014544"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014729.1">
        <property type="protein sequence ID" value="ENSXCOP00000014544.1"/>
        <property type="gene ID" value="ENSXCOG00000011026.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155692"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032394">
        <property type="entry name" value="Anoct_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF45">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16178">
        <property type="entry name" value="Anoct_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="435"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="480"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="653"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="706"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="806"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin dimerisation" evidence="10">
        <location>
            <begin position="42"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="9">
        <location>
            <begin position="261"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="887" mass="104104" checksum="EFD565B81B996115" modified="2018-12-05" version="1">LFVCFCFADDSSGNGVNALCEGGPLPGHSEVKRLQPNRDTVFFRDGVRRIDFVLSYVDDKDERKQERRKEFEANLLKEGLELETEDKSESKDHKTYFLKIHAPWEVLATYADVLNIKVPFKESGMQPGQDVHLDWLSCPFRLPDHIMHPQIDYFTYPFDKNKVDFFLINDKETFFPPSTRNRIVFYILARVPYYREGHKDREKTGVKRLLNNGTYTAAFPLHDCRYWKRARNAECESERFNLYKHWAKFLCFYKEQPLNLIKKYYGEKIGIYFAWLGFYTEMLFFAAVMGVICFTYGALSYGNNKSSEEICDAKIGGSILMCPLCDKKCPFWKLNSTCFSSWQSHLFDNEGTIFFAIFMGIWVTLFLEFWKRRQARLEYEWDLVDFEEEQQQLQIRPEFEIKCTNRRQNKITQEMEPYLPVTSRCARFCFSGATVIFWISMIVACIIGVIAYRLAVYAAFASIIKDPMRKIQLVGRFITPQLATSVTASCINFVIIMILNFFYEKVAIWITDMEIPKTHLEYENRLTIKMFLFQFVNYYSSCFYVAFFKGKFVGYPGKYSYMFGNLTKLRNEECDPGGCLIELTTQLVIVMAGKQVWGNIQEALLPLMRNWWSSRKGRHNPENHSSRWEQDYVLLNFSQIGLFYEYLEMVIQFGFITLFVASFPLAPLLALINNILEIRVDAWKFTTQFRRPVASKARNIGAWQEILNSVAILSVVTNAFIMAFTSDMIPRMVYLYAYSKDASMSGYINNSLSVYNISQIHPDNMPEDNWHNSTTTCRYRDYRYPPGHERQYTHTMQFWHILAAKMAFIIIMEHVVFVVKFFVAWLIPDVPSDVKARIKRERYLIQEFLHKYEVDRLKLQLSASFITEPRSETSLTLDKHEVVSECS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3L1</accession>
    <name>A0A3B5M3L1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Telomeric repeat-binding factor 2-interacting protein 1</fullName>
            <shortName evidence="10">TERF2-interacting telomeric protein 1</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Repressor/activator protein 1 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Acts both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). Does not bind DNA directly: recruited to telomeric double-stranded 5'-TTAGGG-3' repeats via its interaction with terf2. Independently of its function in telomeres, also acts as a transcription regulator: recruited to extratelomeric 5'-TTAGGG-3' sites via its association with terf2 or other factors, and regulates gene expression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
            <location evidence="10">Telomere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the RAP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3L1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017945"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018169.1">
        <property type="protein sequence ID" value="ENSXCOP00000017945.1"/>
        <property type="gene ID" value="ENSXCOG00000013523.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005351"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070187">
        <property type="term" value="C:shelterin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031848">
        <property type="term" value="P:protection from non-homologous end joining at telomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010833">
        <property type="term" value="P:telomere maintenance via telomere lengthening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11655">
        <property type="entry name" value="rap1_myb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000246">
        <property type="entry name" value="Telomeric repeat-binding factor 2-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021661">
        <property type="entry name" value="Rap1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038104">
        <property type="entry name" value="Rap1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039595">
        <property type="entry name" value="TE2IP/Rap1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015010">
        <property type="entry name" value="TERF2IP_Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16466">
        <property type="entry name" value="TELOMERE REPEAT-BINDING FACTOR 2-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16466:SF6">
        <property type="entry name" value="TELOMERIC REPEAT-BINDING FACTOR 2-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08914">
        <property type="entry name" value="Myb_Rap1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11626">
        <property type="entry name" value="Rap1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0010">Activator</keyword>
    <keyword evidence="3 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0779">Telomere</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BRCT" evidence="12">
        <location>
            <begin position="31"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="172"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="201"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="378" mass="42305" checksum="A4F73631674F3E1B" modified="2018-12-05" version="1">QQTADEAGKQPYYPTHSISPVLFLTVEGEPMSFFLRPGPHKQKLQPLIRDGGGILCSVQVPGAILLIDPEENSSVLESDAHRYVSTQYIHDCIKKEEQLDLDDYRLNPKAIQRSSSKLNNSGGRRAGLFLAYATPFPSRVAYTPEEDAAILNFISKRKSETGGNRLWQEMEKQRVTSHSWQSMKSRFKDRLSKNQSASTEVETKEDSNKLPEKKTEAENQASTETTEPEKDEPMTPSSPRKHHLSKNSPADQLKLSTITSSPNRAKEKLEASPVQEKPQRRLTRRQLELEAASSSPEPYGKKLRSDLVSVTKALLKTSGDFTAAADLLLNPSSVSEPFWSRNDDSLLLSADPVGRQKLQEKYGEENVAKRIVFLEVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5W5</accession>
    <name>A0A3B5M5W5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phosphatidate cytidylyltransferase</fullName>
            <ecNumber evidence="5">2.7.7.41</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CDS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M5W5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019568"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019813.1">
        <property type="protein sequence ID" value="ENSXCOP00000019568.1"/>
        <property type="gene ID" value="ENSXCOG00000014709.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158877"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016720">
        <property type="entry name" value="PC_Trfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773:SF4">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01148">
        <property type="entry name" value="CTP_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="122" mass="13999" checksum="FCCBA52F7295AF91" modified="2018-12-05" version="1">FTNLIQLIIRGSDSELKADKEGVSDESKVDSGVPEVPVPPDDTPEVLNKALSGLSSRWKNWWVRGILTLAMISFFFFIIYLGPMVLMMIVLCVQIKCFHEIITIGYNVYHSYDLPWFRTLSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8S6</accession>
    <name>A0A3B5M8S6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Un-named hu7912</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M8S6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020693"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020944.1">
        <property type="protein sequence ID" value="ENSXCOP00000020693.1"/>
        <property type="gene ID" value="ENSXCOG00000015515.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016543"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045216">
        <property type="term" value="P:cell-cell junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058586">
        <property type="entry name" value="Ajm-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038825">
        <property type="entry name" value="Apical_junction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21517">
        <property type="entry name" value="APICAL JUNCTION COMPONENT 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21517:SF4">
        <property type="entry name" value="UN-NAMED HU7912"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26649">
        <property type="entry name" value="Ajm-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Apical junction molecule ajm1 alpha/beta" evidence="2">
        <location>
            <begin position="639"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="458"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="895" mass="102034" checksum="44A79F4CE1251E10" modified="2018-12-05" version="1">MTRTHPPEILASNLYQDITLGDITGHSQFQQQRSRSKSAPRLKATLTPVPISVSPPQARNGRDGSQAVSDFMRTAEIQRESTSKPFLNEVHPIKLQPHTPLYVSDCFEQDKTEKPAVSPHVRCRVDIKPDAAVLEHTSRQIPNTHGEHLWQRYSQASSRSGLYVPRHIASSPTPTPSECYSGDFRQGYLYTTSMSPISYHQEHRAYSNPNIPTKFFYTEDAVRQYDPRTRWRGSYFSQTPLSTAYYGEDSRFTPYNMNSSRSFCSKPLSPEEQYFPPRLYNTEGYRRPRMSQAFSDDWYRSSISGFSSHSSQNTPPRVRQDLSIPPWFTNSYTEASRLGADVKNHSKSWDNIICPRHEREQTVPRGRSYENLFHQARQGTTSDVTAKPVILNLSSSPRRYAALSLSENSLERNSNKPWRNSKSANWFVTPEITITDNDLCAGNARCRDVHSVSWDTSDGDKASTQRLLNEKKQSNTETENTKERKHNNFSLQQSLEQLDELLADLVVDYKPPTSKKSSENLLDQLKQLITEDNDKNTESPGLENLECLSRQLPSSKSSPDTLKDPDSGCDAFQRSAEECSPDHSTDEDDTMACANKKCNRIESMFNACLYFKSCHSCYTFYCSRNCRRDDWEIHKETCLYGRVSSVCRHTLKFCRESSEIHKAFSRIAKAGYLSRGRGVLFLGFANPETAENFLQVGLESLLISPTYLSLRELDGFKDNLGEYCKELQQAGNEYDPNECFLLNVSVAVGELVPNRPSPRVPAPTVRKYAKVSLASSSPDKKVLKKDEEMETLILTPPPGTPDIDKEGEEGRKAREVCFVNIQRELRTRGVFLRHEYPKIYNQLCEFVESNKRFTPTTIYPIDKRTGKQFMCMIMAASEPRTLDWLGTPHLLDDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9C5</accession>
    <name>A0A3B5M9C5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9C5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016364"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016570.1">
        <property type="protein sequence ID" value="ENSXCOP00000016364.1"/>
        <property type="gene ID" value="ENSXCOG00000012361.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002548"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140007">
        <property type="term" value="C:KICSTOR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029982">
        <property type="entry name" value="Kptn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15435">
        <property type="entry name" value="KICSTOR COMPLEX PROTEIN KAPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15435:SF2">
        <property type="entry name" value="KICSTOR COMPLEX PROTEIN KAPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="148" mass="16591" checksum="DF239E6F77900E67" modified="2018-12-05" version="1">FIGSQAVSAQSYPFVEDSFSRFPSQSNIYGLCQAGEQELLAATLKGKVVCFRYQELQHKVRPVAKEVQFTYIPVDAEIVSIDAFNKSSPKRGLVVGITFIKDSGDKATPFLNIYCDYEPGSEFNLESIAQSCLNLELQFTPFQLYHTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAX1</accession>
    <name>A0A3B5MAX1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAX1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020732"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020985.1">
        <property type="protein sequence ID" value="ENSXCOP00000020732.1"/>
        <property type="gene ID" value="ENSXCOG00000015536.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156141"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11308">
        <property type="entry name" value="Peptidase_M14NE-CP-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1120:FF:000009">
        <property type="entry name" value="adipocyte enhancer-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000007">
        <property type="entry name" value="Carboxypeptidase X (M14 family), member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000035">
        <property type="entry name" value="probable carboxypeptidase X1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050753">
        <property type="entry name" value="Peptidase_M14_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532:SF43">
        <property type="entry name" value="CARBOXYPEPTIDASE X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532">
        <property type="entry name" value="PROTEASE M14 CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13620">
        <property type="entry name" value="CarboxypepD_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="F5/8 type C" evidence="16">
        <location>
            <begin position="45"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="17">
        <location>
            <begin position="222"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="14">
        <location>
            <position position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="679" mass="78256" checksum="36B45F5F0AB849F6" modified="2018-12-05" version="1">MVAKKLKNVLKKPKVIPRKPKVTKTPKPEIKKVTHHQSAATKTASQCPPLGLESLRVKDAHLRASSYKRRGLGPHRGRLNIQSGIDDGDIYDGAWCARYKDKNQWLEVDAQRLIRFTGVILQGRNSIWSWDMVQTYKVQFSNDTLLWKPCMNGSKEAIFEGNQDTETPVLSLFDSPTVARYIRINPQTWYQNGTEGDICLRAEVLGCTLPEPTESRDKLDFKHHNYKEMRKLMKSVHDECPNITRIYSIGKSYKDLKLYVMEISDNPGKHELGEPEFRYVAGMHGNEVLGRELLLNLMQYMCREYKRGNQRIVHLVKETRIHLLPSMNPDGYEMAFKKGSELVGWALGRYSYEGIDMNHNFADLNSVMWTAIELETDQSKLINHYFPIPEQYTSEEAFVAPETRAVINWMQNIPFALGANLHGGELVVTYPYDMTRDWAPREHTPTPDESFFRWLAAAYASTNQVMSNPDRRPCHNKDFLRYNNIINGADWHNVPGSMNDFSYLHTNCFEVTVELSCDKFPHASELPIEWENNRESLLVYMEQIHRGIKGVIRDKDTGVGIADATIKVEDIDHHIRSVVDGDYWRLLNPGEYRLTVSAEGYFPSSRTCYVMYDHFPTICDFNLTKTPRRKAKDILAKGGKLPKDLQLRLRQLRMRKLRVTTKAINQRRAAAAGKRAKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAX5</accession>
    <name>A0A3B5MAX5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAX5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020973.1">
        <property type="protein sequence ID" value="ENSXCOP00000020720.1"/>
        <property type="gene ID" value="ENSXCOG00000015530.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000271800"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="125" mass="14020" checksum="0C80B7E5D6DECECB" modified="2018-12-05" version="1">MLCNLRLEAVHSNCYIEPKYSENLLSVTLNWYSSRITQYLAPSIFRSTLSGFSKWCIHHSQLGTSLPPTWKTAAGTSNFLLTGTFFLPPFQGSRVYGVHDSAPLESSWASWLLHILVLSLPTQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCA8</accession>
    <name>A0A3B5MCA8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCA8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017334"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017555.1">
        <property type="protein sequence ID" value="ENSXCOP00000017334.1"/>
        <property type="gene ID" value="ENSXCOG00000013064.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155599"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF50">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE (SDR FAMILY) MEMBER 13A, DUPLICATE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="305"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="307"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="340" mass="37450" checksum="77930C2DF800F4B8" modified="2018-12-05" version="1">SLAKNQEPFLLLALACLIWLDLPRMPSAVILRCRTLGVPAIAMHGYECCFIFLLGNPQVVFMQLDLASLKSVRSFAETFLKTEPRLDILINNAGVISPGCTEEGFDLAFGVNHLGHFLLTNLLLERLWRCGPSRVITVSALLHRFGSIDFQLLAPQTDAVPPQSAWSSLQAYCNSKLCNVLFTRELANRLEGTDVTCYTLHPGVIYTELCCNLSQWLQLLMMPLAKLFLLDPSGGCQTTLYCALQEGIEPLSGRYFSNCALQQVGANGRDDGLAKKREKKKKSQKTKENIYKHFPPHPLLALCPSPNEGTVQTPTGATLNLSLQPNSATTTKKLPAESAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCF9</accession>
    <name>A0A3B5MCF9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCF9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018956"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019193.1">
        <property type="protein sequence ID" value="ENSXCOP00000018956.1"/>
        <property type="gene ID" value="ENSXCOG00000014257.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183033"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039102">
        <property type="entry name" value="FAM13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15904">
        <property type="entry name" value="FAM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15904:SF16">
        <property type="entry name" value="PROTEIN FAM13B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="2">
        <location>
            <begin position="45"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="206" mass="23139" checksum="567E98D1F8D18421" modified="2018-12-05" version="1">WEDVRFQLNCGEEEVAEEEAMRKSVSPLPGNEGEGSASTARVFGVPLEEVTHVHTVSGHEVPALVKHIVDYIEEHGQLDLEGLFLVNGNAERVDWLRQRYDSGEEVELEKEADLASAVSLLRLFLQELPEPVIPTALQGHILQLYQDYSNEEELCRNLKYLLQQLPHVNYGLLRFLCRFLASVASLQPDSWTVGALAAVFGPDIFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCU2</accession>
    <name>A0A3B5MCU2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027898421.1">
        <property type="nucleotide sequence ID" value="XM_028042620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCU2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022073"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022342.1">
        <property type="protein sequence ID" value="ENSXCOP00000022073.1"/>
        <property type="gene ID" value="ENSXCOG00000016497.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114160169"/>
    <dbReference type="KEGG" id="xco:114160169"/>
    <dbReference type="CTD" id="115330"/>
    <dbReference type="GeneTree" id="ENSGT01140000283328"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14990">
        <property type="entry name" value="7tmA_GPR146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047143">
        <property type="entry name" value="GPER1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037487">
        <property type="entry name" value="GPR146"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24226:SF3">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 146-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24226">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 182 AND ESTROGEN RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="24"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="275"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="44"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="333" mass="37862" checksum="7AA4F3CED6D79254" modified="2018-12-05" version="1">MWISMLYNETDAIVDFQLSQDLGLILSILSLVYLLVCFPLGLCYNILLVVVNLSNKVSMTMPDVYFVNMAIAGLVLNLVAPVELLSSTFTRWHAWEYNDEVHITLLILFNISSLVIMYSTTLLSLDYYIERALPRTYMSSVYNTKHVCGFIWGGAVLTSFSSLLFYVCNHISTKMVECSKMQNKEAADAIMMFIGYAVPAVAVLYAFVLILRIRKESTPLDQDSARLDPSIHRLLLASVCVQFVLWTPYYMTLLVHTIASAPGYISNGHYLPSYNFMRCVSKLLAFSSSFAMPLMYRQMNKNFSGKLQRLLRRLHCRDQSCPHEHSAVQQVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEJ5</accession>
    <name>A0A3B5MEJ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEJ5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021975"/>
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        <property type="gene ID" value="ENSXCOG00000016420.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000158663"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:beta3-adrenergic receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:norepinephrine-epinephrine-mediated vasodilation involved in regulation of systemic arterial blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF3">
        <property type="entry name" value="BETA-3 ADRENERGIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="51"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="394"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="394"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="420"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="440" mass="49406" checksum="D74556E77E9C9093" modified="2018-12-05" version="1">CALLNDIPRCASILLKFCKFNYQKEIKTYLNPPGDWFSVMAVIIILVTVVGNLLVIIAVARTPQLQTSTNILIMSLACADLIMGVLVVPFGASYVLIGNWRLGRTFCNLWTSIDILCVTASIETLCVIAVDRYVAITRPLRYTVLLCKFRARVIVCLVWGVSTLISFVPIMMIDWLWKKNESSCYENTNCCDFVVNKEYAISSSLVSFYIPLVIMIAVYAQVFVIAARQVKLIKKARPRLQNEGLAQIQSGSNVNNLLPPACMGSNSFNNVARRKSAKRRPSRLLIGELRAIKTLGIIMGTFTICWLPFFVANIIQAFNETQLNNRLFLYLNWLGYINSGLNPIIYCRSPEFRTAFKNLIGCPWLSTVWLNSFYKELRTRCSCFLWQTESGTVGSFQKSPDSRTDGSGSLGSRSEEMSIGFSQSNGSTQFTDCLEPESQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MF35</accession>
    <name>A0A3B5MF35_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Myocyte enhancer factor 2cb</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
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            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="Ensembl" id="ENSXCOT00000022580.1">
        <property type="protein sequence ID" value="ENSXCOP00000022307.1"/>
        <property type="gene ID" value="ENSXCOG00000016666.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157492"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="F:protein dimerization activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055013">
        <property type="term" value="P:cardiac muscle cell development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:embryonic heart tube morphogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myocyte-specific enhancer factor 2A homolog"/>
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    </dbReference>
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        <property type="entry name" value="Transcription factor, MADS-box"/>
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    <dbReference type="InterPro" id="IPR036879">
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    </dbReference>
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        <property type="entry name" value="MADS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945:SF145">
        <property type="entry name" value="MYOCYTE ENHANCER FACTOR 2B-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
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    </dbReference>
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        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="8">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="329"/>
            <end position="426"/>
        </location>
    </feature>
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        <location>
            <begin position="358"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="372"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="385"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="426" mass="45548" checksum="E8C841851B345355" modified="2018-12-05" version="1">MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSTNKLFQYASTDMDKVLLKYTEYNEPHESRTNSDIVEAVPPSNYDMPVSIPTSNQNNLIYSHPGGSLGNHNLLPLAHHGLQRNSMSPGVTHRPPSAGNAGGLMGADLTTGAGTSAGNGYGNHRNSPGLLVSSGGMNKNMQAKSPPPMNLGMNNRKPDLRVLIPPGAKNNMPSINQRINNSQSAQSLATPVVSVATPTLPGQGMGGYPSAISTSYGTEYSLNSADLSSLSGFNSGSSLHLGSMSGWQQQHLQNMQHSALSQLGNCSSSHLCQGSNLSLPSAQSLHIKSEPVSPPRDRTSSTPGGYGGGVPSSQNPQSRQDSGRSPVDSLSSCSSSHEGSDRDEHRNEFHSPLGLARAALDERESPSIKRVRLSEGWAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MF99</accession>
    <name>A0A3B5MF99_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cyclin-dependent kinase 14</fullName>
            <ecNumber evidence="3">2.7.11.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Cell division protein kinase 14</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MF99"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022983"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023260.1">
        <property type="protein sequence ID" value="ENSXCOP00000022983.1"/>
        <property type="gene ID" value="ENSXCOG00000017176.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157640"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000131">
        <property type="entry name" value="cyclin-dependent kinase 14 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000007">
        <property type="entry name" value="Cyclin-dependent kinase 14, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF154">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 18 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="89"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="50"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="419" mass="46858" checksum="D8746E3ECCD2EE5A" modified="2018-12-05" version="1">MSTRGCQGTDSVIKPLDTIPEDKKVRVQRTQSGFDPFEKPASQVKRVHSENNACITAKSSSTGKESPKLRRHSSPSSPTSPKFGKADSYEKLEKLGEGSYATVYKGKSKVNGKLVALKVIRLQEEEGTPFTAIREASLLKGLKHANIVLLHDIIHTKETLTLVFEYVVSLNSDLSCGNHSSDEYLYLFFLLRGLSYIHQSYILHRDLKPQNLLISDTGELKLADFGLARAKSVPSHTYSNEVVTLWYRPPDVLLGSTDYSTCLDMWGVGCIFIEMIQGVAAFPGMKDIQDQLERIFLVLGTPSEETWPGVNSLPHFKTERFTVYSAKKLRQAWNKLGYVDHAEELASRFLQCFPKNRLSAQAALNHEYFSNLPPRLWELQDMSSIFTVPNVKLQTESGDSIQVCARKNSHGKASSGSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFF9</accession>
    <name>A0A3B5MFF9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
            <location evidence="1 11">Extracellular space</location>
            <location evidence="1 11">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MFF9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022335"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022608.1">
        <property type="protein sequence ID" value="ENSXCOP00000022335.1"/>
        <property type="gene ID" value="ENSXCOG00000016686.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158599"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003144">
        <property type="term" value="P:embryonic heart tube formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19354">
        <property type="entry name" value="Wnt_Wnt9b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000002">
        <property type="entry name" value="Protein Wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF84">
        <property type="entry name" value="PROTEIN WNT-9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 11" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="353" mass="39650" checksum="F7E84BBB7869F23E" modified="2018-12-05" version="1">MAISGCRHPPPILLLLLLLLLIDFYWWLTGREPLVLLPGPFSNEAPSGKAHLKQCEQMSLTRRQKRMCRREPGLAETLRESVRLGQLECRYQFRNERWNCTLDGRGSLLKRAFKETSFLLAVSSAALTHALAKACSSGRMERCTCDDSPGIQHREAWQWGVCGDNLKYSTKFLKKFLGQKRVSKDLRAQVDAHNINVGIRAVKSGLKTTCKCHGVSGSCAVRTCWKQLSPFHDTGRLLKYRYDNAVKVLSVTNAATGETELAGPRRHGQSPRNTDLVYLEDSPSFCRPSRYSPGTGGRSCTKDTSCQSLCCGRGYNTAMRLTSLSCHCQVRWCCHVECQTCVREEEVYTCKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFH4</accession>
    <name>A0A3B5MFH4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Xanthine dehydrogenase/oxidase</fullName>
            <ecNumber evidence="5">1.17.1.4</ecNumber>
            <ecNumber evidence="6">1.17.3.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>hypoxanthine + NAD(+) + H2O = xanthine + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24670"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.17.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>xanthine + NAD(+) + H2O = urate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.17.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>xanthine + O2 + H2O = urate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:21132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
            <dbReference type="EC" id="1.17.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the xanthine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.4" evidence="5"/>
    <dbReference type="EC" id="1.17.3.2" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MFH4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022462"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022737.1">
        <property type="protein sequence ID" value="ENSXCOP00000022462.1"/>
        <property type="gene ID" value="ENSXCOG00000016784.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183114"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004854">
        <property type="term" value="F:xanthine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004855">
        <property type="term" value="F:xanthine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.120:FF:000001">
        <property type="entry name" value="Aldehyde oxidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000015">
        <property type="entry name" value="Aldehyde oxidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.50:FF:000001">
        <property type="entry name" value="Aldehyde oxidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.365.10:FF:000001">
        <property type="entry name" value="Xanthine dehydrogenase oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.50:FF:000001">
        <property type="entry name" value="Xanthine dehydrogenase oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.10:FF:000001">
        <property type="entry name" value="Xanthine dehydrogenase/oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000004">
        <property type="entry name" value="Xanthine dehydrogenase/oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.120">
        <property type="entry name" value="[2Fe-2S]-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.50">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.365.10">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.50">
        <property type="entry name" value="CO dehydrogenase flavoprotein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002888">
        <property type="entry name" value="2Fe-2S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036884">
        <property type="entry name" value="2Fe-2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005107">
        <property type="entry name" value="CO_DH_flav_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036683">
        <property type="entry name" value="CO_DH_flav_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002346">
        <property type="entry name" value="Mopterin_DH_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022407">
        <property type="entry name" value="OxRdtase_Mopterin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014307">
        <property type="entry name" value="Xanthine_DH_ssu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02963">
        <property type="entry name" value="xanthine_xdhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45444">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45444:SF3">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01315">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03450">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00941">
        <property type="entry name" value="FAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01799">
        <property type="entry name" value="Fer2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="MoCoBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01092">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55447">
        <property type="entry name" value="CO dehydrogenase flavoprotein C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47741">
        <property type="entry name" value="CO dehydrogenase ISP C-domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="CO dehydrogenase molybdoprotein N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="Molybdenum cofactor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00559">
        <property type="entry name" value="MOLYBDOPTERIN_EUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="12" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="16" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="22">
        <location>
            <begin position="8"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="23">
        <location>
            <begin position="235"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="835" mass="91368" checksum="B9765404B6BAC9D3" modified="2018-12-05" version="1">MSAQQVGDALCVFINGKKVTENHVDPETMLLPFLREKLRLTGTKSGCGGGGCGACTVMVSRYQPTTKTILHYSANACLLPVCQLYGAAVTTVEGIGSSKTRIHPVQERIAKAHGSQCGFCTPGMVMSMYALLRNKPKPTMDDITQALGGNLCRCTGYRPIVDGCRTFCQEGNCCQANGGANCCLNGEGNTNESEHEKPQLFDQEKFLPLDPTQELIFPPELILMAEAANPQTLTFHGERMTWVTPTSLEELVQLRAKNPKAPLVLGNTNIGPDIKFKGVLHPLIISPIRVKELFEVSEAADGVWVGAGSSLSELQSLLEKMVPQLPEEKTELFRALIQQLRNLGSLQIRNVASLGGNIVSAYPNSDLNPVLAAGNCKVKVVSTGGRREVPLNQDFFVGFGKTILKPEDVVVSVFIPFTRKGEYVRALRQAPRKEVSFATVTTGMRVRFSEGSRVVREVSLYFGGMGPTTVNATKTCKAITGRPWDEKTLNQAYDSLLEELDLPPSAPGGKVEFRRSLTLSLLFKFNLEVLQKLKEANVIKDELPEKLQPLPKEIQPSLQEFQHVPKDQDSQDPVGRPMMHRSAISQATGEAVYCDDIPKTDGELVMVLVTSSRAHAKITLLDVSEALKLPGVVDVITASDVPGKKARPMFGYEQELLAENQVFCVGQTVCAVLADTKTHAKRGAAAVKITYEDLPDPIFTIEDAIEKSSYYEPRRMVSKGDVTEAFKTCDRVYEGKYLQMTDSTGSLEEIVLWFQEAVAETLDIQSNRVTCHVKRLGGAFGGKVIVTSVLASITSVAAWKTNRAVRCVLERGEDMLITGGRHPVLGKYKVRKNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFJ0</accession>
    <name>A0A3B5MFJ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000023093"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023371.1">
        <property type="protein sequence ID" value="ENSXCOP00000023093.1"/>
        <property type="gene ID" value="ENSXCOG00000017239.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158067"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000134">
        <property type="term" value="P:negative regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048812">
        <property type="term" value="P:neuron projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052838">
        <property type="entry name" value="Myosin-XVI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47335">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-XVI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47335:SF1">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-XVI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 5" id="KW-0518">Myosin</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="8">
        <location>
            <begin position="146"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="12"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1223"/>
            <end position="1301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1352"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1498"/>
            <end position="1583"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="947"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1135"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="60"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1227"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1251"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="1282"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1392"/>
            <end position="1415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1553"/>
            <end position="1562"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="236"/>
            <end position="243"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1583" mass="174782" checksum="CA83F40C77984FF8" modified="2018-12-05" version="1">TPPILEAEMALRAEQVNNSKQKRSDSVQSDGGLPSQRHIMTPDSNGQTLSPEPPKQNQQQEKKQEVERQEPEGRQADATGTEDVWYEVGPVWFVHKDGFTRATQLKPDEGTPDLPSGRVRVRLETDGSLHDVTEFEVEKCNPSELDVCEDLSDLQCVNESGVLHTLTSRSKASMPLTHSGPNLVSLWPPLQTPSKTLKTRRGECVWDAPPALAALVKRVYLSLVGTRRDHSVCALGRSGTGKTATCQAFTHTLLKRAGAAGPNIMERVSAMFSILRSFGCVSSPHSDTSSRFATVFSLDFNHAGQVSAGHLQTMMLDKWTVCQKSPGESNFLVFSQMLAGLSSEMRTELQLHQLPDSNSFGILHPTKVEEKQRASVAFTKLLAAMETLNFSAGEQKAIWHVLAAIYHLGAAGACKVGRRQFVNFDSAQAASKVLGCEAEELDTAVFKHHLRQLLQKATGVSRERNEAEEGPRLTAAQCVDGMAAGLYEELFTTIVSLINRALSSEQLALASVMVVDAPGLRNPRHSAEERGASWSELCHNYLQERLLEHYHSHTFTHTLERYAQERIHVAFECPDSRSSEVVSAIDQSPAQVRAADGDLRGLLWVLDEEMVTPSSCESAALQRVCQYYSNTASLLSSLRGVVKAMFSPRISLPRLCRGLAGLEGGSQRALQRNGIIRKTFSGGMAAIRRHSQCIAVKLQADALVNLIRRARPVFLQSVSAKSSSGGLDVSALRVQLHSTQILSALQLYRTGYPEHMTLSDFRCHFQALSPLIMKRYASMFVSHDERKAVEELLVELDVDKNSIVVGASRVFMKRGVLQYLEQQRDQQVTRWLIYLQAACKGHVGRQKYRKLKVRPSPVRCPRAPLKQLEMQLEQEYEEKQMVIHEKHDLEGLIATLCEQVGHRDFDVERKLRRDLKRTHALLADAQLLLATLDSSGQGRPNGSKDQIERLHCQLEESEARRLEAENTQKAVAMELDNAQLELENICRQKSVVDEQLLLLQHEKMDLLKRLEEDQEDLNELMKKHKALIAQSSSDISQIRELQAELEEVKKQRRSLQEELQQCVSRMQFLESSTVGRSIVSKQEARVCDLENKLEFKRGQVKRFEVLVLRLRDSVVRLGEELEQSAQAEAREKEHAGYYQQRLQEMKLEMDELSQREQDSSRRRMELEMQVEELSTVRQTLQADLETSIRRIVDLQAALEEVESSDESETESVQTAVESFTRKKDLDSVSSVGSVGSKDVGEGIRSRRGGSPFSSQDGRQSVTDTMSTYSFRSCLDPDDEGSEAGGVGGAGGARHLSRAPSSSALSELLDGLRKRRASTEVADGAGSAVSLPIYQTTGASTLRRRSSALSLGPADVQELRPGPGILKPPSPLLPRAASSRSVADSEKLPRFNLSGSASSLPSLPRLSSLSSSLASRHPPPPLSIPEEDQDEPLHSAAAPFQRSPQPLRRCMLESVAAATDGGEGSLVSEPMVFQNRRLTGGCDDAASDILPAIRRAQSTSSLASSIRGTRRALSVHFGELPPSTRSRKSSETESSSSGGSGGSSQGSGPRRRLERPQGQRLEAEGSEGGDVASVMKKYLRKEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFL9</accession>
    <name>A0A3B5MFL9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MFL9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022512"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022787.1">
        <property type="protein sequence ID" value="ENSXCOP00000022512.1"/>
        <property type="gene ID" value="ENSXCOG00000016827.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154801"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01369">
        <property type="entry name" value="KISc_KHC_KIF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000009">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF70">
        <property type="entry name" value="KINESIN HEAVY CHAIN ISOFORM 5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="8"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="85"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="332" mass="37225" checksum="CB89F1FBD28B7BBF" modified="2018-12-05" version="1">MVDAAECGVKVMCRFRPLNEAEITRGDKYIPKFKEDDTVVITGKPYVFDRVLPPNTTQEQVYEQCAKQIVKDVLGGYNGTIFAYGQTSSGKTHTMEGKLHDPQLMGIIPRIAQDIFDHIYSMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLAVHEDKNRVPYVKGCTERFVSSPEEVMDVIDEGKANRHVAVTNMNEHSSRSHSIFLINIKQENVETEKKLSGKLYLVDLAGSEKVSKTGAEGSVLDEAKNINKSLSALGNVISALAEGTKTHVPYRDSKMTRILQDSLGGNCRTTIIICCSPSVYNEAETKSTLMFGQRYSSLFLWSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MG66</accession>
    <name>A0A3B5MG66_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MG66"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018594"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018828.1">
        <property type="protein sequence ID" value="ENSXCOP00000018594.1"/>
        <property type="gene ID" value="ENSXCOG00000013994.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182977"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05716">
        <property type="entry name" value="IgV_pIgR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF111">
        <property type="entry name" value="IMMUNOGLOBULIN SUBTYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017185141" description="Ig-like domain-containing protein" evidence="4">
        <location>
            <begin position="25"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="10"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="141" mass="15961" checksum="6A935E91FFB3500A" modified="2018-12-05" version="1">MVLTLSFAVPVLCLLLLMKNKVDSVDLLAPEEVTAAPGGLVEIACQYDLKFKESPKYWCKGSIYELCRILVRTTRKTQHDRCSITDDREAGVFTVTMTSLMERDEDKYWCVISRSGRNVYKSVRFKLDYLTSTGDFPSGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHF9</accession>
    <name>A0A3B5MHF9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Testis specific protein Y-linked</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHF9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019009"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019246.1">
        <property type="protein sequence ID" value="ENSXCOP00000019009.1"/>
        <property type="gene ID" value="ENSXCOG00000014286.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166378"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="329"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="132"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="97"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="350"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="405" mass="45054" checksum="A9D0925AB93FA4A1" modified="2018-12-05" version="1">MQHPSTPVDQSDSAAIAAAEALASLTGGDGEESQETPCSSAKVRPVKHGSKNKQRPGQQPSKVGSKTQAAADSSTSARSTDREDKDDAAEADEGDESVSGSSSTPSSSFPSDNEDNDDGECAIVSVKMAPEMRQSVALLAQVQMRLEALEKKSVRLHQRLELKINRQRRPHLDQRSSITKTIPGFWVTALLNHPHLSAHIDETDEDALSYMTDLEVESFKNTKLGYRIRFHFRRNPYFQNNIIMKELHLGMGGSPMSFSNPILWHRGHNLTANSEPRKSCRGVYETFFSWFSDHSNPVQDDVAQILKDDLYRDPLRYYLTPLWEPRENGSATEARAAGNGNGDDCVVISDSDDEPGEDTGDADRGRNREDEEDDDDEEEEGVEEEEEEEEDGRRASPGRCRNRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJP7</accession>
    <name>A0A3B5MJP7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tryptophan 2,3-dioxygenase</fullName>
            <shortName evidence="10">TDO</shortName>
            <ecNumber evidence="10">1.13.11.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Tryptamin 2,3-dioxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Tryptophan oxygenase</fullName>
            <shortName evidence="10">TO</shortName>
            <shortName evidence="10">TRPO</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Tryptophan pyrrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Tryptophanase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TDO2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Heme-dependent dioxygenase that catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring and converts L-tryptophan to N-formyl-L-kynurenine. Catalyzes the oxidative cleavage of the indole moiety.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>L-tryptophan + O2 = N-formyl-L-kynurenine</text>
            <dbReference type="Rhea" id="RHEA:24536"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58629"/>
            <dbReference type="EC" id="1.13.11.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="10">Binds 1 heme group per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 10">Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9 10">Homotetramer. Dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the tryptophan 2,3-dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.11" evidence="10"/>
    <dbReference type="RefSeq" id="XP_027865374.1">
        <property type="nucleotide sequence ID" value="XM_028009573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJP7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019759"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020005.1">
        <property type="protein sequence ID" value="ENSXCOP00000019759.1"/>
        <property type="gene ID" value="ENSXCOG00000014838.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114139559"/>
    <dbReference type="GeneTree" id="ENSGT00390000008593"/>
    <dbReference type="UniPathway" id="UPA00333">
        <property type="reaction ID" value="UER00453"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004833">
        <property type="term" value="F:L-tryptophan 2,3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019442">
        <property type="term" value="P:L-tryptophan catabolic process to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3810:FF:000001">
        <property type="entry name" value="Tryptophan 2,3-dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01972">
        <property type="entry name" value="T23O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037217">
        <property type="entry name" value="Trp/Indoleamine_2_3_dOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004981">
        <property type="entry name" value="Trp_2_3_dOase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10138">
        <property type="entry name" value="TRYPTOPHAN 2,3-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10138:SF2">
        <property type="entry name" value="TRYPTOPHAN 2,3-DIOXYGENASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03301">
        <property type="entry name" value="Trp_dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140959">
        <property type="entry name" value="Indolic compounds 2,3-dioxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="1 10" id="KW-0349">Heme</keyword>
    <keyword evidence="5 10" id="KW-0408">Iron</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0823">Tryptophan catabolism</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="405" mass="47829" checksum="EA8B9007F2C9BC1C" modified="2018-12-05" version="1">MSRCPYFEKKHLLFKSKPSIEESQDASQAGADKDSKGRIIYGDYLQLDKITTAQVLQSELQGNKIHDEHLFIVTHQAYELWFKQILFELDSVREIFISGHVRDERNMLKVNTRIHRIVMIFRLLVDQFAVLETMTALDFFDFRTYLTSASGFESLQFRLLENKIGVQDNLRVPYNRRHYKDYIKGDDTETLLETEQEPTLLKLVEEWLERTPGLEVDGFNFWERLEINIFDGLNVEEKKIEKMPESEEKEKLMVEVVQQREHFMSLFDEKRHEHLVSKGERRLSYKALQGALMIYFFREEPRFQVPFQLLTSLMDMDTLMTKWRYNHVCIVHRMIGSKAGTGGSSGYHYLRSTVSDRYKVFVDLFNLATFLVPRHWVPKLNPNVHTFLYMAECCDSSYCSSEDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKI5</accession>
    <name>A0A3B5MKI5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MTSS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKI5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021651"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021915.1">
        <property type="protein sequence ID" value="ENSXCOP00000021651.1"/>
        <property type="gene ID" value="ENSXCOG00000016190.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183156"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034334">
        <property type="term" value="P:adherens junction maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032233">
        <property type="term" value="P:positive regulation of actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07643">
        <property type="entry name" value="I-BAR_IMD_MIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000010">
        <property type="entry name" value="Metastasis suppressor 1, isoform CRA_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013606">
        <property type="entry name" value="I-BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030127">
        <property type="entry name" value="MTSS1/MTSS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15708">
        <property type="entry name" value="ACTIN BUNDLING/MISSING IN METASTASIS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15708:SF10">
        <property type="entry name" value="PROTEIN MTSS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08397">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51338">
        <property type="entry name" value="IMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMD" evidence="9">
        <location>
            <begin position="1"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="WH2" evidence="8">
        <location>
            <begin position="707"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="254"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="336"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="600"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="255"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="474"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="619"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="735" mass="79802" checksum="09582D1C688A375D" modified="2018-12-05" version="1">MEAVIEKECSALGGLFHTVIGDMKSSCPIWEDFITKAGKLQSQLRATAVAVTIFLDAFQKVADLATNSRGGTRDIGSALTRMCMRHRSIEAKLKQFSMCFLEGLINPLQEQMEEWKRGVNTLDKDHAKEYKRARQEIKKKSSDTLKLQKKAKKGRGDFQPQLNSAMQDVSDKYILLEETEKQALRKALIEERQRFCCFVALLQPVVDEEISMLAEVTHLQTISEDLKALTSDPHKLPPASEQVISDLKGSDYGWSYQTPPSSPSTTMSRKSSMCSSLNSVNSSDSRGSSGSHSHSPSSSSSSSSSHHLFHHHHPCHRYRSSTLPLQAPVRLSSISSHDSGFISSSQDQNASSKSSSPMPAETKLSNGFDHYSPASPSYLHSNGGSLGSAPGTSFPFFSPSSPSPSTSSPTRSWSRPASALLPDYPNYCTLGSPMVPSSKVPSWKDWAKPGPYDQPMVNTLRRRKDKEALAGVENNGSINGGNSPPSSQTSISADDLEAQNELALALSRGLELDTQRSNRDSIQCSSGYSTQTNTPCCSEDTIPSQISDYDYFSMQPSDFDKSSTIPRNSDIGQSYRRMFQTKRPASTAVIVTPGVATIRRTPSSKPCARRSGSVGGGPIPIRTPVVPVKIPTVPDMSGTVNGSRSSEEIGGGEESSYSSTFVRPDEAGTLPLMSWSGQASTNPPTVPLPNQLVQPYQGSGEEADKQDEGNMLVAIRKGVKLKRTLTNDRSAPRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5ML48</accession>
    <name>A0A3B5ML48_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027880234.1">
        <property type="nucleotide sequence ID" value="XM_028024433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5ML48"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024767.1">
        <property type="protein sequence ID" value="ENSXCOP00000024473.1"/>
        <property type="gene ID" value="ENSXCOG00000018280.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114148919"/>
    <dbReference type="KEGG" id="xco:114148919"/>
    <dbReference type="GeneTree" id="ENSGT01030000234640"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF847">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="193"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="316" mass="36899" checksum="A9FF2A22DC585533" modified="2018-12-05" version="1">MNNEMNATYIFLDGYVEMSKYKYAYFFLMFSAYILIICSNLTIVYLIWANKNLHEPMYVFIAALLVNCVFYSSTTYPKLLIDLLSEKQRITYEACLLQFYFFYFVGPAEFFLLAVMSYDRYVSICKPLLYPTIMTNTTVTVFLVFSWFVPACHASIPLIMLSSTKVKLCSFNIRGIFCNNAVYTLQCVMPRSLAIFGVFAILDIVILPILFILFSYTKILIISYRSSKEVRRKAAETCLPHLLVLFSLTLLSIYDICIVRVESDFSKTARLIMTLQIVLYHPLFNPFIYGFKMKEIYKHLKRLFWQAKVVLSKNQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MM65</accession>
    <name>A0A3B5MM65_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Syntaxin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">STX2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MM65"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020564"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020814.1">
        <property type="protein sequence ID" value="ENSXCOP00000020564.1"/>
        <property type="gene ID" value="ENSXCOG00000015406.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234627"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048787">
        <property type="term" value="C:presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031629">
        <property type="term" value="P:synaptic vesicle fusion to presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15882">
        <property type="entry name" value="SNARE_syntaxin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00179">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000068">
        <property type="entry name" value="Syntaxin 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028671">
        <property type="entry name" value="STX2_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF36">
        <property type="entry name" value="SYNTAXIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00804">
        <property type="entry name" value="Syntaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00503">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="249"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="5">
        <location>
            <begin position="176"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="278" mass="31575" checksum="BD09DEE39BB88985" modified="2018-12-05" version="1">MKDRLAELAAVSATSAWADTASFICLLFRLIKVEEVRGLIDKISYQVEEVKKLHSTILSAPNPDHSTKSQLDGLTNDIKGNANVVRTKLKCEFSCDCCTFLSCHSVLSRKFVEVMTQYNETQVSFRERSKGRIQRQLEITGRVTTNEELEEMLESGNPSIFTSDIISDSQITRQALNEIESRHQDIMRLESSIRELHVMFMDMAMLVETQGDMVNNIENNVSNAAEYIYRAKEETKKAVRYQKKSRRKYMILAFALLILLAVIALIVGLSVGLTKRPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNA7</accession>
    <name>A0A3B5MNA7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Neurexophilin and PC-esterase domain family, member 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NXPE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNA7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025583.1">
        <property type="protein sequence ID" value="ENSXCOP00000025278.1"/>
        <property type="gene ID" value="ENSXCOG00000018878.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182866"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057106">
        <property type="entry name" value="NXPE4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026845">
        <property type="entry name" value="NXPH/NXPE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16165">
        <property type="entry name" value="NXPE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16165:SF9">
        <property type="entry name" value="NXPE FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06312">
        <property type="entry name" value="Neurexophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24536">
        <property type="entry name" value="NXPE4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NXPE C-terminal" evidence="2">
        <location>
            <begin position="334"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="567" mass="64774" checksum="E1705F67C57EF3FA" modified="2018-12-05" version="1">MYQNLSKYALLFLFLSLSGFIFLLRNIHTLESWNCHAISAFYQFQTRIHSSLVSVNLPTYNQNLTFCAHLGQKPSPEDKLEEHYLLDSIAWPGPPARFAPLSLRQTSDPVHSLFTILPNKERREWRIGDQLEALVQMHDYQGRPKRYGGDFLLARLHSPELGAGVAGTVVDHRNGIYSALFPLLWVGAAQVEITLVHSSEAVAVLKHLREHRPDRVFFKSLFRLGFLSETTVCNMCLPSDQHPLCNYTDLYTGEPWYCYKPKMLGCDTRINHAKGGYLKHLLTNQEALLFQSGVNIKVQIHTSGKDRINVLPPEGKVYTSLFSNPSGYYLKDSWRPLGGTVMRHFNESAAITQCLRNKVINMYGDSTVRQWFEYLITLVPEMKEINLHSPKNVGPFMAVDTTHNILLKYRCHGPPIRFSTVLSSELRYIFNELDGLSGGPNTVVVLSIWAHFSTFPVEVYIRRLRHIRRALLRLLGRAPETLVVIRSANLQALDEEVSLYNSDWYSLQLDTVLKAMFRGMDVLMVDAWEMSLAHHLPHALHPPRAIVQNMIDMLLSYVCPVTKSRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNL9</accession>
    <name>A0A3B5MNL9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Rho guanine nucleotide exchange factor (GEF) 7a</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNL9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025136"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025441.1">
        <property type="protein sequence ID" value="ENSXCOP00000025136.1"/>
        <property type="gene ID" value="ENSXCOG00000018771.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155360"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030032">
        <property type="term" value="P:lamellipodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01225">
        <property type="entry name" value="PH_Cool_Pix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046376">
        <property type="entry name" value="PH_Cool_Pix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46026:SF3">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46026">
        <property type="entry name" value="RHO-TYPE GUANINE NUCLEOTIDE EXCHANGE FACTOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16614">
        <property type="entry name" value="RhoGEF67_u2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="79"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="351"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="279"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="402" mass="45453" checksum="B8CD898C2AD521F6" modified="2018-12-05" version="1">MCPMPSLYLRRRMVHKINTNKLQAHTEERNPYTQAQKEAPTLGAKELHPCLVFIQVVHLPLHNCLLNCTHVNEGLVSCQVLIHPYFSVSLSFYFEQEKNERYLMLFPHILLMLSASPRMSGFIFQGKLPLTGMIVTKLEDCEAHKNAFELNGTMFDRLQVVCANQQDLQDWVEHLTRQIKHSAATAPSHKPLAVPCHTLPSHPLTPSRHAESRAMTVAPTYHTLPHPSSHGTTHSTMTWGPLEPPNTPKPWSLSCLRPAPPLRPSAALCYKEDLSKSPKSVKKLLPKRKPERKQSEEEFALRKSTAALEEDAQILKVIEAYCTSAKTRQTLNSTWQGTDLMHNHVLADMDQSCMDSPGRRSSVSRPELSSDLSEDSDYDSIWTSHSYRMGSVPRKSCISHQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNR0</accession>
    <name>A0A3B5MNR0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MIT, microtubule interacting and transport, domain containing 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNR0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025181"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025486.1">
        <property type="protein sequence ID" value="ENSXCOP00000025181.1"/>
        <property type="gene ID" value="ENSXCOG00000018810.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010868"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061952">
        <property type="term" value="P:midbody abscission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02685">
        <property type="entry name" value="MIT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.30">
        <property type="entry name" value="MITD, C-terminal phospholipase D-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052817">
        <property type="entry name" value="MIT_domain_contain_protein1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032341">
        <property type="entry name" value="MITD1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038113">
        <property type="entry name" value="MITD1_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21222">
        <property type="entry name" value="MIT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21222:SF1">
        <property type="entry name" value="MIT DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16565">
        <property type="entry name" value="MIT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MITD1 C-terminal phospholipase D-like" evidence="1">
        <location>
            <begin position="32"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="180" mass="20921" checksum="442B46E1DB9A4395" modified="2018-12-05" version="1">MDRAEQIKSHVNQMKEDGKYHEQIKISEDATGYCYEALFKPYISSALSEIWVEDPYIRHTHQLYNFLRFCEMLLKSCGKVNKIHLLTSQDEGDNTQQNSALSELRGSLGGHGVTLDVQYSSTIHDREIRFDNGWIIKIGRGLDYFKKPKGRFSIGYCDYDLRHCHETSVDIFHTKHTKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQF5</accession>
    <name>A0A3B5MQF5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Josephin-2</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Josephin domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Cleaves 'Lys-63'-linked poly-ubiquitin chains, and with lesser efficiency 'Lys-48'-linked poly-ubiquitin chains (in vitro). May act as a deubiquitinating enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027869454.1">
        <property type="nucleotide sequence ID" value="XM_028013653.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027869455.1">
        <property type="nucleotide sequence ID" value="XM_028013654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQF5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026008"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026321.1">
        <property type="protein sequence ID" value="ENSXCOP00000026008.1"/>
        <property type="gene ID" value="ENSXCOG00000019435.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114142385"/>
    <dbReference type="KEGG" id="xco:114142385"/>
    <dbReference type="CTD" id="126119"/>
    <dbReference type="GeneTree" id="ENSGT00390000009228"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.40:FF:000003">
        <property type="entry name" value="josephin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040053">
        <property type="entry name" value="JOSD1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006155">
        <property type="entry name" value="Josephin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13291">
        <property type="entry name" value="JOSEPHIN 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13291:SF2">
        <property type="entry name" value="JOSEPHIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02099">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01233">
        <property type="entry name" value="JOSEPHIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01246">
        <property type="entry name" value="Josephin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50957">
        <property type="entry name" value="JOSEPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Josephin" evidence="12">
        <location>
            <begin position="3"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="184" mass="20750" checksum="9135CAEF2EBBAF37" modified="2018-12-05" version="1">MSDGEVFHEKQRLELCAIHALNNVLQERVFTKETADDICKRLAPQCVVNPHRSVLGTGNYDVNVIMAALQSRDLAAVWWDKRRTVQSLCMSKVQGFILNVPSRVSLGIVSLPLRRRHWLAVRQVNGQYYNLDSKLKGPACIGGEAELRAFLSEQLSHEVAEMLLVVQRDVEEDGSWLNPDSPNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQK2</accession>
    <name>A0A3B5MQK2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Stanniocalcin</fullName>
            <shortName evidence="7">STC</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Corpuscles of Stannius protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 7">Its primary function is the prevention of hypercalcemia. Upon release into the circulation, it lowers calcium transport by the gills, thereby reducing its rate of influx from the environment into the extracellular compartment. STC also stimulates phosphate reabsorption by renal proximal tubules. The consequence of this action is increased levels of plasma phosphate, which combines with excess calcium and promotes its disposal into bone and scales.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the stanniocalcin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027881785.1">
        <property type="nucleotide sequence ID" value="XM_028025984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQK2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021769"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022033.1">
        <property type="protein sequence ID" value="ENSXCOP00000021769.1"/>
        <property type="gene ID" value="ENSXCOG00000016271.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114149882"/>
    <dbReference type="KEGG" id="xco:114149882"/>
    <dbReference type="CTD" id="393511"/>
    <dbReference type="GeneTree" id="ENSGT00390000005989"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004978">
        <property type="entry name" value="Stanniocalcin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11245">
        <property type="entry name" value="STANNIOCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11245:SF7">
        <property type="entry name" value="STANNIOCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03298">
        <property type="entry name" value="Stanniocalcin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0372">Hormone</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017485442" description="Stanniocalcin" evidence="8">
        <location>
            <begin position="18"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="256" mass="27959" checksum="8D9692A38B52BB87" modified="2018-12-05" version="1">MLRRSAPLLLLCSLCLASGFQMPPEEAAPRRARFSSSSPTDVARCLNGAMAVGCGFFSCLENSTCDTDGMHEICELFLHAAATFNTEGKTFVKKSLQCISQGISSKVFQAIRRCNVFQTMIAEVQEECYTSLDICTVARTNPDAIGEVVQVPTHFPNRYYSTLLQTLQACDEQTVSAVRTGVMTRLGPDMESFLQLVQNKPCTPSSDSTAFSNPASWKNMPVFNIQPGFRGRDPTHLFAKRSADTQGNEGGAQADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQV5</accession>
    <name>A0A3B5MQV5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GMP phosphodiesterase delta subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PDE6D/unc-119 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQV5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023571"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023852.1">
        <property type="protein sequence ID" value="ENSXCOP00000023571.1"/>
        <property type="gene ID" value="ENSXCOG00000017607.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014595"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.40:FF:000001">
        <property type="entry name" value="protein unc-119 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.40">
        <property type="entry name" value="GMP phosphodiesterase, delta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051519">
        <property type="entry name" value="PDE6D_unc-119_myristoyl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008015">
        <property type="entry name" value="PDED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037036">
        <property type="entry name" value="PDED_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12951:SF3">
        <property type="entry name" value="PROTEIN UNC-119 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12951">
        <property type="entry name" value="RETINAL PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05351">
        <property type="entry name" value="GMP_PDE_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="GMP phosphodiesterase delta subunit" evidence="7">
        <location>
            <begin position="84"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="243" mass="27710" checksum="3374C3C170EC4B8F" modified="2018-12-05" version="1">MSGAKARSDAPVAENVSGGGHGSAAPTRDRKPGGGVLKRLKSRRSQVESRPVTEEELRTRSGHMSPEDVLGLRVATRGYLCKPEDNIYNIDFVRFKIRDLETNTVLFEIAKPPHSDDDEEHSEGDGTAGRFVRYQFTPAFLRLRTVGATVEFTVGNRPLNNFRMIERHYFRDHLLKSFDFDFGFCIPSSRNTCEHIYEFPQLSETLVRQMVECPYETRSDSFYFVDNRLVMHNKADYAYNGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRN7</accession>
    <name>A0A3B5MRN7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 592</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRN7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027074"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027407.1">
        <property type="protein sequence ID" value="ENSXCOP00000027074.1"/>
        <property type="gene ID" value="ENSXCOG00000020222.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158357"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045914">
        <property type="entry name" value="Zn532-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041697">
        <property type="entry name" value="Znf-C2H2_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057356">
        <property type="entry name" value="Znf-C2H2_ZNF592"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47222">
        <property type="entry name" value="ZINC FINGER PROTEIN 532-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47222:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16622">
        <property type="entry name" value="zf-C2H2_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25412">
        <property type="entry name" value="zf-C2H2_ZNF592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="666"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="753"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="817"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="899"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="929"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="1053"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="107"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="209"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="325"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="846"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="990"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1081"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="158"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="336"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="387"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="413"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="847"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="868"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="999"/>
            <end position="1012"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1135" mass="126411" checksum="04C7B3E832BFAB8F" modified="2018-12-05" version="1">MGDMKTPDFDDLLAAFDIPDATGLDSKEAIQESHEEIDNQLKHSVICLDDSLLSNPVATTADVPLVSVIVKNTSRQESLEGFSDRRHSAPGLQNGYKGQEACIKPVEPSSTSFSNSFQADLNGESSRNLPGGIPLQQKPDETAVFSPSLSHFSPPYSPQTEALYSKNQKVSKQEGPSCCPFTSLSSEQNFKETESQNTEDVTNSYSSAKFQTSELPSSNDAPIAPKTKKDPIENTDPTESSASHSDCVKANNMAPISPRSPRSPNEVVKRLMKPSDSPVSLCSDSSGKTSPAVASVSSPAIPRVRIKTIKTTSGQIKRTVTSVLPDSETDEIHSAYESSPSQSMISEDSFCNVSPRQSQAADCSPGSNPACTSSPKMPRRSEENSRRFRTGFHRSSRPVQRSVAHDVQKPKRVSSTTGHTSNTNFLPKAMHLASLNLVPHSVAASVAARLSSHQQSQHILSSSVCSTVPLVHQVKTATPFPRSSAPNTAAGTLNRLLTSANPVPVYVPNLSPPPECNIKLPPHGYCCLECGDSFGVERSLAFHYNRRSVQIEVGCTHCAKTLVFFNKCALLAHAREHKSNGSVMQCTQLHLKPISEQQMFVPLSSESNTGPYPSTLVSPVSEPVMPLYPDSVIRNRFRCLECNKQLPDYKALAGHYQKMSEDVEGLICKVCSMLLPNKCSFRTHQRLHAHKSPYCCPECGALSRSADIQKHVKENCLHYARKAWYKCLHCDMVFRTLLGQKSHVEEKHCEILYKCSVCPVAFKTSKGCEVHLKNKHKSSKQTPQLVFKCSCKALFNKKHNFFQHFHENANKRVTCVFKCPECNSVFPQKRLLMQHFKNVHVGNLTAETEKDSKEKEGLDWFPESHPVQQEKSHITRKNTDSPRKKSEQDAKSRVKPRGWTCGECLQCFGERESYVSHMKINHGKSMKKYPCRHCELSFNTATSLRRHIRSEHDGKRRIYSCWYCTDPKTTFTTSVMLKNHISLMHGIKNPDLRQMPKTSIKEQVDTSDRSDLDAPSAKHLKTQFRCSKCGFITDDSTEFHQHIPQHKTDENTPQCLHCGLCFTSVVSLNRHLFIVHKVKDPKEDEEEQEMRDVGRSAGSAATAHVNDFKPSLDEEPENPPYTNTSDFHLKLKTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MT16</accession>
    <name>A0A3B5MT16_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enhancer of polycomb homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">EPC1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the enhancer of polycomb family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MT16"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026641"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026965.1">
        <property type="protein sequence ID" value="ENSXCOP00000026641.1"/>
        <property type="gene ID" value="ENSXCOG00000019896.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155003"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024943">
        <property type="entry name" value="Enhancer_polycomb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009607">
        <property type="entry name" value="Enhancer_polycomb_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14898">
        <property type="entry name" value="ENHANCER OF POLYCOMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06752">
        <property type="entry name" value="E_Pc_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Enhancer of polycomb-like N-terminal" evidence="10">
        <location>
            <begin position="7"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Enhancer of polycomb C-terminal" evidence="9">
        <location>
            <begin position="588"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="310"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="367"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="465"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="682"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="775"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="477"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="489"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="788"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="814" mass="90339" checksum="EA2EF805270067AE" modified="2018-12-05" version="1">MSKLSFRARALDASKPLPVFRCEDLPDLHEYASINRAVPQMPTGMEKEEESEHHLQRAISAQQVYGEKRDNMVIPVPEAESNITYYDSLYPGDYKMPKQLIHIQPFSLDTEQPDYDLDTDDEAFVNKLKKKMEISCLQFEEMIDRLEKGSGQQAVSLPEAKLLLKEDDELIKEVFDYWSRKRKNSKANSLIPTVKQEKRDGSSTGDPYVAFRRRTEKMQTRKNRKNDEASYEKMLKLRRDLSRAVTILEMIKRREKSKRELLHLTLEIVEKRNSMSDFGGEIMAEVLAERALVRPQIIPLVPLTNQYRHQDHLDSKDYKSKPEKAEPQRPKRRYEKKPKMLPLSSGTAHHAGPVVFNAKDLNQYDFPSSDDEPYSQLHSGSSEAEEENDPDGAYAFRRRAGCQYYARPDLVGSWPWSGAWDGGLAEARFRYSLTTLTVPRRCLGMARRRVGRGGRVLLDRAHTDHDDSFHQLDPETPDLPPPASPPPSATSHSPAPDALAGTSETNTSDRSSPPSAPPPSSDLSQILSNIKSCRWRHFRPRTLPLHELDNAQPLFRRISRGVKRPLAASCGIKTCVFPRWLTFSSVSAFTAEQYQQHQEQLSLMHKQQLEQIQQQHQANSTANSTQVGSLANTLDEASAHFAASALVSAEQLLALKTKDELALGGGVNGVLSSGVYKGLSLSSTASPSPAAPATPSATPTSALLHPCSTALGSATSNNNGTAHPPANATQILLGNNSLRLAIPTGKRIHAPRTLSATMSTSALKLAHAATANCQKPKVAASSTSPLDKVPRENLEQDKPALNSLSENTVAMEVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MTH0</accession>
    <name>A0A3B5MTH0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">HEPACAM family member 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MTH0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027058"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027391.1">
        <property type="protein sequence ID" value="ENSXCOP00000027058.1"/>
        <property type="gene ID" value="ENSXCOG00000020211.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01130000278319"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052280">
        <property type="entry name" value="HEPACAM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44888:SF1">
        <property type="entry name" value="HEPACAM FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44888">
        <property type="entry name" value="HEPACAM FAMILY MEMBER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017398340" evidence="13">
        <location>
            <begin position="22"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="143"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="236"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="452" mass="51328" checksum="752084C7A1D334FE" modified="2018-12-05" version="1">QLRRALFFSNLIMSFISDVGCELIHIRSSVHHGTKGKDLLLSVDARFPLDEAEIQGTWSHTKVSGASTTLVTFTKDSKIIDMTYRKRLDFREPNASLSIRNLKEDDEGYYKLSLNIEFRNEKGKVKKEERTIHVTVDEPVSRPIIEKKPSHTVIEDKANVTWTCSVEKGTRVVFRWLRDNVSLTASERYHFTQDKSMLLISPVRKEDKGTYRCAVSNPVSQIIESRGVELNVYYGPYNLEVNSVQGLRTGKVFTINPGELVYFECQADSNPPNSYVWISKSPNATKIITTGPQLQVKSNELAQTEDYLCRAFNNVTKKQDEAQFTLVVASLRTGGWRAEYGRTESMSLLAVIAVCSFIIGFMLLFLFRRACHPKRDHLPYYDSYNIKCVCTGHEDAAEDFGIYEFVSIPGKMDSTQASCKSLVRLESIQDMHTTIYDVIRHVPESPSASLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MU39</accession>
    <name>A0A3B5MU39_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">CCR4-NOT transcription complex subunit 4</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">CCR4-associated factor 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">E3 ubiquitin-protein ligase CNOT4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Potential transcriptional repressor NOT4Hp</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">RING-type E3 ubiquitin transferase CNOT4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="32 33">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Has E3 ubiquitin ligase activity, promoting ubiquitination and degradation of target proteins. Involved in activation of the JAK/STAT pathway. Catalyzes ubiquitination of methylated RBM15. Plays a role in quality control of translation of mitochondrial outer membrane-localized mRNA. As part of the PINK1-regulated signaling, upon mitochondria damage, ubiquitinates ABCE1 and thereby recruits autophagy receptors to the mitochondrial outer membrane to initiate mitophagy.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with CNOT1 via its C-terminus but does not stably associate with the CCR4-NOT complex. Interacts (via RING domain) with UBE2D2. Interacts with ABCE1, PINK1 and PELO.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MU39"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027924"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028260.1">
        <property type="protein sequence ID" value="ENSXCOP00000027924.1"/>
        <property type="gene ID" value="ENSXCOG00000020824.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000068"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16618">
        <property type="entry name" value="mRING-HC-C4C4_CNOT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12438">
        <property type="entry name" value="RRM_CNOT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000006">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000044">
        <property type="entry name" value="Putative ccr4-not transcription complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034261">
        <property type="entry name" value="CNOT4_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039780">
        <property type="entry name" value="Mot2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039515">
        <property type="entry name" value="NOT4_mRING-HC-C4C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603:SF0">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14570">
        <property type="entry name" value="zf-RING_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16 27" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 26" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15 25" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13 26" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 26" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="29">
        <location>
            <begin position="14"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="30">
        <location>
            <begin position="109"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="31">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="26">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="256"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="352"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="439"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="595"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="27">
        <location>
            <begin position="68"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="28">
        <location>
            <begin position="361"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="28">
        <location>
            <begin position="491"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="28">
        <location>
            <begin position="595"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00057081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00062432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00071435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00075062"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00077837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00083547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00083942"/>
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    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="651" mass="70910" checksum="1736EB4D96F338E2" modified="2018-12-05" version="1">MSHSPELKDDPMECPLCMEPLEIDDVNFFPCTCGYQICRFCWHRIRTDENGLCPACRKPYPEDPAVYKPLSQEEIQRIKNEKKQKQNEKKQKVTENRKHLASVRVVQRNLVFVVGLSQRLADPEVLKRPEYFGRFGKIHKVVINNSTSYAGSQGPSASAYVTYIRSEDALRAIQCVNNVVVDGRTLKASLGTTKYCSYFLKSMQCPKPDCMYLHELGDEAASFTKEEMQAGKHQEYEQKLLQDLYKINPGFLQPPACGTEKSKSKSNSAQSPNDKPPESIGMVNGETLQQVKQSDLLCAVCLPLLLQMPTSDSPSPPPGLTKPILVVPISMSDLSARSPFEGAAAESQSLFSDNSNFRHPNPLPAGLPPFPSSSRGSSDWPMTPEPQILFTSDTIPVSSSTDWQAAFGFGSSSKQQDDDLGFDPFDVTRKALADLIEKELSVQDQSPSSPGPLAHGSGHSPGLPPSNLSTSHHFPGGLPRVHPLHHRAIYSSFSFPGGHNSQAGQQQQPPATRHPWMGIPTRNNLAHLNHSASAASHSNILDLNLPPQHNTGLGGIPISGTKNSSSIDGLNVKEWQDGLRALLPNININFGGLPNSSSSSSSSSSNSVNHIGGPTGPAGISHSLSWDSTASWMDPAIITGKNVGWTRLLNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUZ3</accession>
    <name>A0A3B5MUZ3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tensin 1b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MUZ3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027356"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027689.1">
        <property type="protein sequence ID" value="ENSXCOP00000027356.1"/>
        <property type="gene ID" value="ENSXCOG00000020426.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155400"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010761">
        <property type="term" value="P:fibroblast migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1110:FF:000002">
        <property type="entry name" value="tensin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051484">
        <property type="entry name" value="Tensin_PTEN_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45734">
        <property type="entry name" value="TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45734:SF3">
        <property type="entry name" value="TENSIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="1">
        <location>
            <begin position="4"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="C2 tensin-type" evidence="2">
        <location>
            <begin position="178"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="43922" checksum="6C51C9D37CAD7588" modified="2018-12-05" version="1">MFVLQAMEESYEVDLVYITERIISLSFPAAADERSYANNLKEVATMLRSKHGEHYLVLNLGEQRNDLTKLNHKVLEFGWPDHHAPALDKICSMCKAMDTWLSGDQRNVVLLHNKVTKHSGVRIGRKICENARNADQALDRFAMRRFYEDKALPVGQPSQRRYVQYFNGLLSGDIKINNKPLFLHHVIMHGIPNFESKGGCRPFLKIYQAMQPVYTSGIYNVQGDSNTSICITIEPGLLLKGDILLKCYHKRYRNPTRDVVFRVQFHTCAIHDLGVVFGKSELDETFKDGRFPEYGKVEFVFSYGPEKIRGLDHLENGPSVSVDYNTQDPLIRWDSYESFSHPSEDAVDTENGMSSHTPYSWSKQFECGTFYEQAAIWVRQNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MV82</accession>
    <name>A0A3B5MV82_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">KIAA0319-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MV82"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027718"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028053.1">
        <property type="protein sequence ID" value="ENSXCOP00000027718.1"/>
        <property type="gene ID" value="ENSXCOG00000020668.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161462"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029865">
        <property type="entry name" value="KIAA0319-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056502">
        <property type="entry name" value="KIAA0319-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46182:SF1">
        <property type="entry name" value="DYSLEXIA-ASSOCIATED PROTEIN KIAA0319"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46182">
        <property type="entry name" value="FI19480P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23620">
        <property type="entry name" value="KIAA0319"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KIAA0319-like C-terminal" evidence="1">
        <location>
            <begin position="43"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="137" mass="14985" checksum="5DCA39DD67CC192A" modified="2018-12-05" version="1">MVTDAQGRVSTATATVEVRPGRPPLSAGSAEVLRVNGPLGCLAQRDTLVRQLAALIHVLDRDIRVRALTVLQFWVQGPTGPIPASSLVVLLRKQLLRDKSDFLLFKVLRVDTVGKAALCHRFCSSLSWTESLDQPRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVZ3</accession>
    <name>A0A3B5MVZ3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histone deacetylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVZ3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000028018"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028359.1">
        <property type="protein sequence ID" value="ENSXCOP00000028018.1"/>
        <property type="gene ID" value="ENSXCOG00000020900.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160061"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019213">
        <property type="term" value="F:deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000005">
        <property type="entry name" value="histone deacetylase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF43">
        <property type="entry name" value="POLYAMINE DEACETYLASE HDAC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="5">
        <location>
            <begin position="28"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000028018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="571" mass="62937" checksum="79985A2EBB85B37C" modified="2018-12-05" version="1">STMGTALIYDEEMTKYKLLWVDPACKIEVPERLSVSYQALVRTGLSDRCVAVPPREASDAEILLVHSEEYLEGVKKTPHMTLEDLKEFTLQYGDVYFHPNIYHCAKLAAGSALQLVDSVMTGTVRNGMALVRPPGHHSMRSAASGFCVFNNVAIAARYAKQKHGAKRVLIVDWDIHHGQGVQYCFEDDPSVLYFSWHRYEHQKFWPNLRESDYDSVGKGSGAGFNINVPWNKVCSSINLNDYLSAFCHVLLPVAYEFCPDLVLVCAGFDSAVGDPEGEMCATPDIFAHLTHLLMNLAGGKLCAVLEGGYNLTSLAQSVCQTVQTLLGDPLPRLANLGSPCERSGQTQTERCRKSRNVFLLPGNYALLRPYLSHEMKTSVEFAATGNSDLSPTALFTKALQKRLSAGIPILLRKKISVLRKMLVVQISGEDKEEQKCKYYIPMCLKKGCGDTVGFIQAVLGLLLPLGYEYDPGLVLLSRMPGSGVGDCTWQQLIGLLKGFAKGHTLVLMQTNDGACMGPTASVLLGHPAPPLQELLTRLPDDGDALERMRERLQADWKMLETLFFLHGMLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWF5</accession>
    <name>A0A3B5MWF5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Small ribosomal subunit protein uS13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">40S ribosomal protein S18</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Component of the small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWF5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025747"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026057.1">
        <property type="protein sequence ID" value="ENSXCOP00000025747.1"/>
        <property type="gene ID" value="ENSXCOG00000019243.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012691"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.50:FF:000002">
        <property type="entry name" value="40S ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.910.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="entry name" value="30s ribosomal protein s13, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_uS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="Rbsml_uS13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_uS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S13/40S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="151" mass="17648" checksum="09EA8E91D76CB1E8" modified="2018-12-05" version="1">MSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLSKRAGELTEEEVERVVTIMQNPRQYKIPDWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWLRVRGQHTKTTGRRGRTVGVSKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWI0</accession>
    <name>A0A3B5MWI0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 15">E3 ubiquitin-protein ligase listerin</fullName>
            <ecNumber evidence="5 15">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">RING-type E3 ubiquitin transferase listerin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">E3 ubiquitin-protein ligase. Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the ribosome quality control complex (RQC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the LTN1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5 15"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024034"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024319.1">
        <property type="protein sequence ID" value="ENSXCOP00000024034.1"/>
        <property type="gene ID" value="ENSXCOG00000017945.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016055"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16491">
        <property type="entry name" value="RING-CH-C4HC3_LTN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000038">
        <property type="entry name" value="E3 ubiquitin-protein ligase listerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001251">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039795">
        <property type="entry name" value="LTN1/Rkr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054477">
        <property type="entry name" value="LTN1_E3_ligase_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056241">
        <property type="entry name" value="LTN1_HEAT_5th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054476">
        <property type="entry name" value="Ltn1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054478">
        <property type="entry name" value="LTN1_UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039804">
        <property type="entry name" value="RING-CH-C4HC3_LTN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12389:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE LISTERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12389">
        <property type="entry name" value="ZINC FINGER PROTEIN 294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22958">
        <property type="entry name" value="Ltn1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24618">
        <property type="entry name" value="LTN1_E3_ligase_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22999">
        <property type="entry name" value="LTN1_E3_ligase_6th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23009">
        <property type="entry name" value="UBC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 14 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="1679"/>
            <end position="1726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1730" mass="193917" checksum="C73156F6F98019D7" modified="2018-12-05" version="1">MGGKNKQRTKGNVRPSSSGRAAEVLAREGGAIPGFVGFDTTVTSELSYVPAVHGAEEIDNLVDADFRLVLRKLSKRDVVTRLKAVQDFGAMCQERDAEEVKGVLPYWPRIYCKISVDPDRRIREATQQAFEQLVLKVRRSLAPFLKSLMGHWILSQCDTYTPAASAACQAFQAAFSPAKQPEALSFCKDEILTVLQDVLLKETADTLSDSQSVTEEERETKYFRMLTSSLLGVKKLLSLLLQDDRAALQQRLALLVNSGKFWKYSKHKTPQIRGAFFEMQCALCEFTPDMVQSEAARLCPAVLLSIDDTDPVVLPHVWEAVLHVMSTIPDCWTHVNAKKGFLPKLWALLKEGGKGMAKGLHPNLMPLLSKLPQQITDPDLDFYTTFYTAFLQGLSSERAATSPSESAVIVTSFVECLRYSITQNAGDEQSQEKIRSMLISQQLLPLIEKAIRNPSLQNGPLFLLITEMLISWEKRAGLHCEGEKNNNKDVFKRLLADFWEELGLLCVRFVDDEDVDLQALEGVATLLQVMRNPERISKKQTQKKKSVRLCLTKTEEEEKSVIQGEGVEKFAQTVSEPVQEKALGSLQTQHFEDAVCQLAQLCLAHVNEKNSEKHLVFLSLLLQSFHTPRIFSTLLEFDEIKPGDDKWTGEKNPAVQFLLRRVVVWLAEVGRSDTEHLVEMVFSSLSCCSQEETTQILNHITTMGLEWRIILQIIQRAGKDPKTRASCRNWLKGSILGEQLLGLTAELCKMEHNSTAPKTPTSNNSWTLIGLVLSQNNNNESLIGEVYMEKMLEKLHTTLSETKSLSDAGNMEPLISFICDVASSFFCSVQDCLLLLSAEELLLTVFQLTAQDQRHTHLSESLLDKLKKVCAAGVRSLVQHCECEVRQGGFLHSAAVWVMKQLLTSNLDIKGLQVLVRAVQSLVETIVSVGGQASSVLSQFFALLTPSQTEWTRIRHALSPQWVKSPLLSNRHRGVCEKPSKDMWKSRDSNRLPAHLCVCAVLGIVAQTAAVLASGQKEAPCSSFLPNLTGTVSEVLYALQWCKELDFRPPVASAYRNLLAEWGLSAGLHSKVPMKSLLETLYSRSDSPRAFFHFSNMNLSKLSTLQVLCPLMAKEDRETLIALATAGIINWQEEENVYGSLAVLLCCLNANTPVEDEVIQAVLATLMEWRNSREDWFLFSCDLSDTTSEQLNLNVEMIRFLCWSVTHCPAVLGGNQWDFLLCSMLAWLETASENVSCLWNPWVQLFVCENAALIVTLNQFFAAPSSDVLEKLPTELAGEWKDFFIEGIYNLLLPLPVRITEAFPEPDDPVFPMAVLQSVGLALTCVPVQQLNQNSLPPRFIADQKTNLPEPLQTLLNTLCPLLLFKARPLQVTVHHMLEKVMPQLPECDGEGDSNRSDDDRDEPCLSPPAALMAILSTCEELCDSILAALQVGEFAVVQPLSVEYSCILAYLLAWKLLLTFFKSSPSHLRAHYAQYLKRSCSLNKLLLHLFKLMPENPICPGQGTETKESKTFFTESLLLTWELPHLACSVYYSTVQDLPAMVRLWWNSQEKRVSSAVEKFTSKYVSPVLSAQEISSVHDSTQMFDSMTVKARSAAREVIAIYSVDDIYIELVIQLPQNYPLGSITVESGRRVGVAVQQWRNWMLQLSTYLTHQNGSIMEGLALWKNNVDKRFEGIEDCMICFSVIHGSNYSLPKKACRTCKKKFHSACLYKWFTSSNKSTCPLCRETFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXA1</accession>
    <name>A0A3B5MXA1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">EEF1A lysine methyltransferase 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54192"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13826"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:54193"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXA1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024545"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024841.1">
        <property type="protein sequence ID" value="ENSXCOP00000024545.1"/>
        <property type="gene ID" value="ENSXCOG00000018337.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016366"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019369">
        <property type="entry name" value="Efm5/EEF1AKMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041370">
        <property type="entry name" value="Mlase_EEF1AKMT1/ZCCHC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13200">
        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13200:SF0">
        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10237">
        <property type="entry name" value="N6-adenineMlase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="156" mass="17298" checksum="02BFA36535DC3821" modified="2018-12-05" version="1">MVWLTGDKLHLCKKGLIACVSAPSVYQKLKQGVVDGSGRVSAVVLEYDRRFAAYADDFIFYDYNEPLSLPSGVAPRSFDIVLADPPYLSEECLSKVAQTVQYLSKGKVLLCTGATMENVAKELLGVKMCSFLPKHNRNLSNEFRCFVNYPSDLLCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXG2</accession>
    <name>A0A3B5MXG2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">MDM4 regulator of p53</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the MDM2/MDM4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXG2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024680"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024979.1">
        <property type="protein sequence ID" value="ENSXCOP00000024680.1"/>
        <property type="gene ID" value="ENSXCOG00000018438.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063539"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17673">
        <property type="entry name" value="MDM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16784">
        <property type="entry name" value="mRING-HC-C2H2C4_MDM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051652">
        <property type="entry name" value="MDM2_MDM4_MUL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016495">
        <property type="entry name" value="p53_neg-reg_MDM_2/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12183">
        <property type="entry name" value="MITOCHONDRIAL UBIQUITIN LIGASE ACTIVATOR OF NFKB 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12183:SF37">
        <property type="entry name" value="PROTEIN MDM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006748">
        <property type="entry name" value="p53_MDM_2/4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DM2" evidence="11">
        <location>
            <begin position="23"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="10">
        <location>
            <begin position="270"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="207"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="351"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="238"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="251"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR006748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="456" mass="50567" checksum="EE7BAA0059DF4552" modified="2018-12-05" version="1">MSSLSAQPQASSSSCRTLPGEGNQVKPKAPLLQILRVAGAQEDVFTLKEVMHYLGQYIMGKQLYDKQRQHIVHCQDDPLGELLEVESFSVKNPPVYEMLKKYLVVLGCSAAENLSVGRECVEGGVEDRGQSLEGLPRSACKRPKLDVTLEEWDLSGLPWWFLGNLRNNYSRRSNGSTDIHTNQLSLAQEEDTAIVSDTTEDLWILTEGESEQVSVEMKEATLEEGSGGEEGSVDDDYGGGKEEKSDREMQEEPDEDSQCLSDDTDTEISTQDAWQCTECRKYNIPVQRYCVRCWALRKNWYKDVPRLAHSLSVPDIPACSSLSAQDEEDDSDTGIDVPDCSRTISDPVILPSHSTADRPIPTAGMSKGKGPRPSGFLKHGQLSEGESQESLGMEVQDVRPEALLEPCKLCRVRPRNGNIIHGRTAHLLTCFPCARRLHKFQAPCPGCGKIIQKVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXJ1</accession>
    <name>A0A3B5MXJ1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fibronectin type III and SPRY domain containing 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">FSD2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXJ1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025029.1">
        <property type="protein sequence ID" value="ENSXCOP00000024730.1"/>
        <property type="gene ID" value="ENSXCOG00000018472.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158441"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050617">
        <property type="entry name" value="E3_ligase_FN3/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099:SF6">
        <property type="entry name" value="FIBRONECTIN TYPE III AND SPRY DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="159"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="2">
        <location>
            <begin position="318"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="520" mass="58686" checksum="0A20E130DD4BC7A2" modified="2018-12-05" version="1">MKLGKCWTLSLICFQGEITSTRNQLQGKVAEMENFAGHLEEIFLTVEENFGRQEQHLEQHYNDVLQTLSQRYDDRETGLQEEKKGKLESLYNQLVACGQALDTSKELIETAQEVYRCQDKRLFLKVTQLAILTCSLVVASITRVDWLCICVIQTAPSAPVINPQLPNSATQTSLHVCWSLFSDDTVEYYELYYRPVLEDTPADESKVKVKETHCTVTELLPNAQYELWVTATNTTGISPASEKALYMTVPSPPVIKQRECSSCPEAALIRWESGNTNPVDSYTVELSETGTVVAVPTCQCLIQLQAGRQYLISVRAVNIGGPSDRSDAITISTTGTFFYLLENTAHPCLSMSEDGFTIFYGDEELPITAMAFEDSTFTRCVAILGDLIPVRGRHYWEVEVDDETEFRVGVAYEDTERDAYLGANGSSWCMRHIRTPSRHKYEFLHNGWSPDLRITVHPVRIGVALDYDRGTLSFFNANLEQHLHTFHCHFHNYVHPCFSLDNPGALTVHNGIQAPDYTFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MY38</accession>
    <name>A0A3B5MY38_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MY38"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026747"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027074.1">
        <property type="protein sequence ID" value="ENSXCOP00000026747.1"/>
        <property type="gene ID" value="ENSXCOG00000019978.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155469"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030175">
        <property type="term" value="C:filopodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005547">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060002">
        <property type="term" value="F:plus-end directed microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030705">
        <property type="term" value="P:cytoskeleton-dependent intracellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051489">
        <property type="term" value="P:regulation of filopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051724">
        <property type="entry name" value="Actin_motor_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040640">
        <property type="entry name" value="MyoX_N_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46049">
        <property type="entry name" value="AGAP003327-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46049:SF2">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18597">
        <property type="entry name" value="SH3_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 5" id="KW-0518">Myosin</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="6">
        <location>
            <begin position="41"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="135"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="249" mass="28333" checksum="A653D83B4AFD6624" modified="2018-12-05" version="1">CVSSCSGGVVVFATDYGQVYTYKQNALTRQKVQPMHHSSVRGVEDMATLEDLHDGAIMHNLYQRYHQRQIYTYIGSILAAVNPYQPLPGLYDRPTIELYSRHHLGEISPHIFAVANDCYRSLWRRQQNQCVLISGESGAGKTESTKLILRFLSAMSQHSLEVSCRDKTSHVEEALLESSPIMEAFGNAKTVYNNNSSRFGKFVQLHFSQKGNIQGGRIVDCILSNTLRVRWTETHFLFLYLEKVLEKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KN67</accession>
    <name>A0A3B5KN67_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KN67"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001553"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001578.1">
        <property type="protein sequence ID" value="ENSXCOP00000001553.1"/>
        <property type="gene ID" value="ENSXCOG00000001259.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156078"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042491">
        <property type="term" value="P:inner ear auditory receptor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042472">
        <property type="term" value="P:inner ear morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF745">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 6" id="KW-0518">Myosin</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="7">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="86"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="129" mass="14758" checksum="E66B2ED905F527D9" modified="2018-12-05" version="1">MYLNEATLLNNVRVRYSKDKIYTYVANILIAVNPYYDIPKLYSVDTIKQYLGRSLGTLPPHVYAIADKAYRDMKVLKMSQSIIVSGESGAGKTENTKFDIDERIELVHSRFGKFVEIHFNEKVTCSISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQL8</accession>
    <name>A0A3B5KQL8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL52</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L52, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL52 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQL8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000767"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000777.1">
        <property type="protein sequence ID" value="ENSXCOP00000000767.1"/>
        <property type="gene ID" value="ENSXCOG00000000647.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005763"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034596">
        <property type="entry name" value="Ribosomal_mL52"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34090">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L52, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34090:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18699">
        <property type="entry name" value="MRPL52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="80"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="80"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="98"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="109" mass="12540" checksum="83920F4D41FB03AA" modified="2018-12-05" version="1">FSSTCGVQAGQKWRLEHGLARSGTEYGPMTDLPDWSFEDGRPAPPLKGQIRRRQEKETLARRIVMLNSEVDHGVEAWNKKQEEARRTEEHRKSLLLKPKGKLLMKKSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRX8</accession>
    <name>A0A3B5KRX8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
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    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRX8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001255.1">
        <property type="protein sequence ID" value="ENSXCOP00000001237.1"/>
        <property type="gene ID" value="ENSXCOG00000001020.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234557"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF18">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="23"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="136" mass="15315" checksum="1B6B530C23971D1F" modified="2018-12-05" version="1">FIPFVGRNYFIIWKQSSVRLEFSKILFNIVNKLSYVMAAEGGSATLNCLYNTSASNHYLYWYRLEEGSSPIFILSRFKIGDGKTVEKRFSSSLDATARSVPLTIQDLRPSDSAVYYCALASLPVNPLKHAVLHSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KSN7</accession>
    <name>A0A3B5KSN7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KSN7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001497"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001520.1">
        <property type="protein sequence ID" value="ENSXCOP00000001497.1"/>
        <property type="gene ID" value="ENSXCOG00000001206.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:response to bacterium"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF133">
        <property type="entry name" value="IMMUNE-TYPE RECEPTOR 5 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
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        <location>
            <begin position="17"/>
            <end position="123"/>
        </location>
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            <begin position="167"/>
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        </location>
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            <dbReference type="ARBA" id="ARBA00004236"/>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="296" mass="33956" checksum="C6B5FD94DA427C89" modified="2018-12-05" version="1">MKNCSYKDIGLWLFVTPLFIMLFSFLKYDVDTLVPVTTVPLGEPATFTCSLPAEKLNNNELHWYKQNAGQTLTLIVKLRKHAEPAYGPEFSASRVNASKNEGFRSLTILRTVQEDEGMYHCAIIDWIENSWHGTYLLLTGKILSRVLKKCIFCSFIYLIRAFLPDHESNSLTLQCSVMSDFNHNACPEDISVFWFKVKSDQSHPNVIYVHGDRNDQCQKKFDFQRRCVFSKNIRASDTGKYYCAVASCGEIFLGTRDNDNQNLDYAALQFSGRKSTRWTKKKQSNTEEGVYAQVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KSS8</accession>
    <name>A0A3B5KSS8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
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    <dbReference type="AlphaFoldDB" id="A0A3B5KSS8"/>
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    <dbReference type="Ensembl" id="ENSXCOT00000001012.1">
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Si:dkey-126g1.7"/>
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    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
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    </dbReference>
    <dbReference type="Pfam" id="PF14484">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
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        <property type="match status" value="3"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="7">
        <location>
            <begin position="137"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="902" mass="102098" checksum="F035F434B59090ED" modified="2018-12-05" version="1">MFWSCLLHSAINAQRDSQNDGASLLKHLLLCKKDIFSSIHLCFCLSPDFGLQEDLDKHRNQLKRRCKHVTEGADELGARTLLNSIYTELYITEGQREEVNTQHEVKQLETASKALSDTSIRCHDIFKAFPDQQGAIRVVLTNGVAGVGKTFSVQKFTLDWAEGLENQDVSVVVLLSFRELNLIRDEQHSLLTLLHVFHPTLQKLPAEQLAVCKLLFIFDGLDESRLSLDFNNSQLVSDVTQKSSVNVLLTNLIKGNLLPSALIWITSRPAAANQIPPSCISRVTEVQGFTDAQKEGYFRKRFSDEELSSRIISHIQTSRSLHIMCGIPVFCWITAVVLENMLTSEQRGELPKTMTDMYSHFLLVQTKKKKNKYYGGHETSQQELMEADREVLLQLGKLAFEHLEKGNIVFYEEDLEQCGLDVTEVSVYSGVCTEIFKRKSVIFQKPVYCFVHLSIQECMAADFLDGSPNTHVGRDLKMEDDNFTLNCFLERVMMKSLQSKTGHLDMFVRFIHGLSLKSNQKLLGGLVDQTENSPENIYKIINNLKKMNTNKMSPDRSINIFYCLMEMNDLSIQQEIQEFLRMENRLERELSEIHCSALAYMLQMLEEVLDELDLDMFNTSKEGKMRLIPVVRNCRKARLVGCRLSETHCEVVASALMSSRSQLIELDLTGNVLPGPGVKLLCDGLASPHCCQKSAVIVWSSLMSNPSHLKHLDMGLNNKLQDSGMKLLCAFLENSESRLKTLSLTGIACAYLASALKSNPSPLTYLDMSGNNLQDSGVKHLCAFMETPNCKLQKLSLWGCRLSGTSCASIVSALNTNPSFLKRLELRDNNLQDSDVEQLLDLVESPQFRLQRLRMEAVIYHYCSLIFPLQMEMMILLQMKRRGSEEVKLDSRLTGKTYLVCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KT10</accession>
    <name>A0A3B5KT10_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">THD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tumor necrosis factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KT10"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001657.1">
        <property type="protein sequence ID" value="ENSXCOP00000001632.1"/>
        <property type="gene ID" value="ENSXCOG00000001326.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000176155"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006052">
        <property type="entry name" value="TNF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11471">
        <property type="entry name" value="TUMOR NECROSIS FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11471:SF34">
        <property type="entry name" value="TUMOR NECROSIS FACTOR LIGAND SUPERFAMILY MEMBER 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00229">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00207">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50049">
        <property type="entry name" value="THD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="THD" evidence="6">
        <location>
            <begin position="98"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="234" mass="26692" checksum="619C202886350C42" modified="2018-12-05" version="1">MFFNFKSGVGRMAHDPSVFVVDTHATRPAVPPRLGKQKKQSGVAQTLLFVLVTLALLGVFFEAFFIYRLYQSEYVSITDSKEMPDTTDPDGRLLPSKPVAHLTDAQDAHHDPHVMAWSVIAEPLLYEMKYKDRHLIIQKEGYYYIYSKVYYSQGGAFCHSVMMRTKMYAGKGISLLEAREYLSKKDKEDKSFSNSFLAGVFYLSQNDAIYVNVSSTKQIISHRSYENVFGAYMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KT51</accession>
    <name>A0A3B5KT51_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Growth differentiation factor 10b</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KT51"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001677"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001702.1">
        <property type="protein sequence ID" value="ENSXCOP00000001677.1"/>
        <property type="gene ID" value="ENSXCOG00000001271.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157214"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045669">
        <property type="term" value="P:positive regulation of osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19394">
        <property type="entry name" value="TGF_beta_GDF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000008">
        <property type="entry name" value="Bone morphogenetic protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF265">
        <property type="entry name" value="BMP3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0339">Growth factor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017252888" evidence="11">
        <location>
            <begin position="21"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="12">
        <location>
            <begin position="317"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="430" mass="48526" checksum="A4FC573534D4C6AE" modified="2018-12-05" version="1">MATWLFHIWSLLVILAPGGAHLARQVARTRPEVDERVSAQESASRDAVSVNMFKLHERYSEEPRSHAAGNTVRSFKAVPRVLHGKELLQFNLSSIQNSEIILSASFHFLNKRPRHYQRPWHLRKPRHLAIGVRHLPSSQLLFYGSSSNSGFAIPLGNISLMSLKKGSWQTRDVTTVVRQARDVNELVITVEVDVGFQHLSPANQPYILVYADDQAIDEPNSVALSLQRYGPFPVGDDAAASTSASRIRRELQLQIETNDIPEVHFNTLKNHELWQNTYFPDKAKAEVTNGRKHGQVSSEAMRKPQVLNFDERTMKKARRRQWSEPRVCSRRYLRVDFADIGWSEWVLAPKSFDAYYCAGTCGFPIPKLAHPSNHATIQSIVRAVGIVPGIPEPCCVPDKMNSLSVLFLDPDRNMVLKVYPGMSVDTCGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KUI4</accession>
    <name>A0A3B5KUI4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KUI4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002172"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002203.1">
        <property type="protein sequence ID" value="ENSXCOP00000002172.1"/>
        <property type="gene ID" value="ENSXCOG00000001730.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00890000139503"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034663">
        <property type="term" value="C:endoplasmic reticulum chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000028">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639:SF34">
        <property type="entry name" value="CHAPERONE PROTEIN DNAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45639">
        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="128" mass="13365" checksum="C4B7F70F36551B09" modified="2018-12-05" version="1">MSGEISMIVIGLDLGTTFCSVGVFLPGSGEVEVIEDEEGRKSIPSAVSFTSTAVLAGYEAMDVADSNPQNTVYDAKRFIGKIFESDVLEQESARYPFQVGQAGARYNGRVQCAGGGPGGRDSGCVSAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KV23</accession>
    <name>A0A3B5KV23_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0046 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KV23"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002411.1">
        <property type="protein sequence ID" value="ENSXCOP00000002377.1"/>
        <property type="gene ID" value="ENSXCOG00000001899.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007681"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051693">
        <property type="entry name" value="UPF0046_metallophosphoest"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12905">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12905:SF31">
        <property type="entry name" value="METALLOPHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="105" mass="12152" checksum="B0DD3D2629901414" modified="2018-12-05" version="1">MPYGDVFIHAGDFTELGLPSEVKKFNDWLGTLPYDIKIVIAGNHELTFDQEFMADLIKQDFYYFPSASKLKPENYENVQSLLTNCIYLQDSEVTVRGFRIYGSPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KVR1</accession>
    <name>A0A3B5KVR1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">May play a role in the development of the central system.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PHYHIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KVR1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004254.1">
        <property type="protein sequence ID" value="ENSXCOP00000004208.1"/>
        <property type="gene ID" value="ENSXCOG00000003307.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014563"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042868">
        <property type="entry name" value="PHYHIP/PHYHIPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045545">
        <property type="entry name" value="PHYIP/PHIPL_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15698:SF8">
        <property type="entry name" value="PHYTANOYL-COA HYDROXYLASE-INTERACTING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15698">
        <property type="entry name" value="PROTEIN CBG15099"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19281">
        <property type="entry name" value="PHYHIP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="322" mass="36345" checksum="83CE25AAFD992D09" modified="2018-12-05" version="1">MELHNVTCDSFKISWNMESRGQDRITHYFVDLNKKEDKSSNKFKHMDVPTKLVAKAVPLPMTVRGHWFLSPRTEYTVAVQTATKQPDGEYAVSAWSEIVEFCTADYSTVQLKQLLLKAEAIAGRMLPFTVFYRNQQREYFQPNRYAQCCRMLPSVKDNSGSHGSPISGKLEGIFFSCNTEFNTGRPPQDSPYGPYRFQIQADLLFNQNTNAYFGDFYCMYTSYHYVIVVLAPSGSKGDIFCRGKLPALDRSNNCFLSCGEEEDGSLSFRHAQDVILEVIYTEPVDVSLGTVSQISGHQLMSLSTLNAKKDPSCKVCNISVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KWW9</accession>
    <name>A0A3B5KWW9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Troponin C, skeletal muscle</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the troponin C family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KWW9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000069"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000069.1">
        <property type="protein sequence ID" value="ENSXCOP00000000069.1"/>
        <property type="gene ID" value="ENSXCOG00000000054.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153541"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000107">
        <property type="entry name" value="Troponin C, skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF57">
        <property type="entry name" value="TROPONIN C2, FAST SKELETAL TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="16"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="113" mass="12603" checksum="7043E73C537C9FC5" modified="2018-12-05" version="1">KPTDAQTDARSFLTEEMISEFKAAFDMFDTDGGGDISTKELGTVMRMLGQNPSREELDAIIEEVDEDGMDSAVLWFEARKSKHDSSDGGVMHGLDVFLLQHISSEVMNELIRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXD2</accession>
    <name>A0A3B5KXD2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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    <sequence length="77" mass="8670" checksum="1712CFCB0C414095" modified="2018-12-05" version="1">MKYFKPEIINPSCCALCQKVLMDPVSTSCGHWFCRQCIRFYWDQSGPSSCPQCGERPRTGAGLLTANQSISDCEIQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1D6</accession>
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            <taxon>Poeciliidae</taxon>
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        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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        </source>
    </evidence>
    <sequence length="362" mass="40922" checksum="F1466B3161B284B6" modified="2018-12-05" version="1">VCTPYSYSPGINKVDYSATFSTLGLISNLIAFIVLIKSFKQTKNHSRSFFLIFLGGLVVTDFMGLLVTGSFVISFYVTRVDWSKSDPACHFCNFMGMSMVFYGLSPLLLGATMATQRFIGINHPFASSSKMTKRRTTSMMLMVWFVAGSISLLPLIGFGSYHIQTPGSWCFLNMTPKTSDWGFALLFSLVGLISIATSILLNTVSVVTLLKVYCGAERRQRSRDHEAEMMLQLILIMTIATVCWCPLLVFIAQTAFARTGFEVKYLLLCIRFATWNQILDPWVYILFRRSVLRRVNPRSDCSPRDRPLSLWPVLQIHSAREIYGKIEASAEIWWQRSLETAINSLAKPDEAQKGFAVMFDFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1E1</accession>
    <name>A0A3B5L1E1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1E1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003746.1">
        <property type="protein sequence ID" value="ENSXCOP00000003706.1"/>
        <property type="gene ID" value="ENSXCOG00000002907.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000283002"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="57" mass="6014" checksum="1B941D9D491F349C" modified="2018-12-05" version="1">LSITVTIMFAVPDFGGFPPSTAVTVSLMVGCCSRSKAFRSTSSADTLSSPLWISKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1H4</accession>
    <name>A0A3B5L1H4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Bicaudal D homolog 2 (Drosophila)</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BicD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1H4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006153"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006224.1">
        <property type="protein sequence ID" value="ENSXCOP00000006153.1"/>
        <property type="gene ID" value="ENSXCOG00000004754.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154471"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008093">
        <property type="term" value="F:cytoskeletal anchor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034452">
        <property type="term" value="F:dynactin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070840">
        <property type="term" value="F:dynein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072393">
        <property type="term" value="P:microtubule anchoring at microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018477">
        <property type="entry name" value="BICD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31233">
        <property type="entry name" value="BICAUDAL D FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31233:SF13">
        <property type="entry name" value="BICAUDAL D HOMOLOG 2 (DROSOPHILA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09730">
        <property type="entry name" value="BicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="796"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="111"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="450"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="818"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="831" mass="94788" checksum="BA6755EA1BA18705" modified="2018-12-05" version="1">MSMEEQDYPEAVLVTEAGPQWLRVEVDRLTRELRETTHEKIQAAEYGLAVLEEKQQLKQRFDEMETEYETVRQELDQLKEAFGQAHSTHRKVAADGESREESLIMESASKEALYQQKVLELQNDLRQAKASLSSAQAENDRLSSIALEMREVNLYVAELQRAQLRDDIREYKVREARLLQDYTELEEENISLQKQVSVLRQNQVEFEGLKHEIRRLEEDSQCVQSQLEDAMRLREIAERQLTEALETIKTEREQKATLRKELSHYMTIGGSVYSSSFNISIDNPKLHEDPSTIHEPDNDDLIRGFENGLVKMGDSDEDNRTLVNKRGEAFKPAPSLVDDLLSELNISEIQKLKQQLAQVEREKMALMNSLQENQKQLEQAYGTVSEQKETVNRLTENLSAMRKLQASKERQSALDTEKDRDSHDDGDYYELDINGPEILQCKYTVAVSEAGELRQELKTLRAQYEECRTKYEDERARLESDVQDLRSKLFSLEKINQADKVEVSRLEKELRLATAAAGESLGSLNVAQDELIAFSEELANLYNHVCMCNNETPNRVMLDYYKDGKALMRRGQEGKEQQTVLLTSGLMGENDNGKSESCSIVVTAAAPEHRPEPMNIYNLVAIIRDQIRHLQQAVDRTTELSHQRLANLELSAVADKDKEACMEEILKLKSLLSTKREQIATLRAVLKANKQTAEVALANLKSKYDSEKAMVTETMMKLRNELKALKEDAATFSSLRAMFATRCDEYVTQLDDMQRQLAAAEDEKKTLNSLLRMAIQQKLALTQRLEDLEFDHEQARRNSATAAGGKGKAKGKGASSSHHVSQMVNCRTHSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L348</accession>
    <name>A0A3B5L348_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">S-adenosyl-L-homocysteine hydrolase NAD binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L348"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004790"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004848.1">
        <property type="protein sequence ID" value="ENSXCOP00000004790.1"/>
        <property type="gene ID" value="ENSXCOG00000003761.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182981"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000035">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000009">
        <property type="entry name" value="Adenosylhomocysteinase like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF3">
        <property type="entry name" value="S-ADENOSYLHOMOCYSTEINE HYDROLASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017247768" description="S-adenosyl-L-homocysteine hydrolase NAD binding domain-containing protein" evidence="5">
        <location>
            <begin position="17"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="6">
        <location>
            <begin position="37"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="284" mass="31722" checksum="3FE9386091613B44" modified="2018-12-05" version="1">MCFVFFLSRLYQLSKAGKLCVPAMNVNDSVTKQKFDNLYCCRESILDGLKRTTDVMFGGKQVVVCGYGEVGKGCSAALKALGAVVYVTEIDPICALQACMDGFRVVKLNEVIRLVDVIITCTGNKNVVTRDQLDRMKNGCIVCNMGHSNTEIDVASLRTPELTWERVRSQVDHVIWPDGKRVILLAEGRLLNLSCSTVPTFVLSITATTQALALIELYNAPEGRYKQDVYLLPKKMDEYVASLHLATFDAHLTELTDEQAKYLGLNKNGPFKPNYYRYLCTLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L3A5</accession>
    <name>A0A3B5L3A5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homodimer. Interacts (via extracellular N-terminal domain) with CBLN1 (via C1q domain), and more weakly with CBLN2; the interactions mediate the trans-synaptic adhesion complexes also with neurexins and are required for ligand-gated cation channel activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Postsynaptic cell membrane</location>
            <topology evidence="20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRID1 subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSXCOP00000004336.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
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        <property type="entry name" value="Glutamate receptor, ionotropic, delta 1"/>
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        <property type="entry name" value="Periplasmic binding protein-like II"/>
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        <property type="entry name" value="ANF_lig-bd_rcpt"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 20" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
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        <location>
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        <ligand>
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        <location>
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        <ligand>
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        <location>
            <position position="625"/>
        </location>
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        <location>
            <position position="652"/>
        </location>
    </feature>
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        <location>
            <begin position="38"/>
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        </location>
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            <dbReference type="ARBA" id="ARBA00004651"/>
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            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="702" mass="78556" checksum="770DD4B698364CFE" modified="2018-12-05" version="1">VAGLILKPESIICECLMVKSDQTTKISFSFNQCLTDACELMNQGILALVTSTGCASASALQSLTDAMHIPHMYIQRNGDGSPRTACQFNPSPGGQRYTLAARPPVRLNNVMLTLMEELRWQKFILFYDIEYDIRGLQGFLDQTSRQGVDVALQRVDRNISKVFTNLFTTMRTEELNRYRDTLRRAILLLSPQGAHTFIHVELQLISQCSHFLFDSCDTEILELTHSALGRMTVIRQIFPLWKDSSTRCMRQNHRISSLLCDPQEGYLQNLEVSNLYLYDSVLMLANAFYRKLEDRKWHSMASLNCIRKSTKPWNGGWSMLETIQKGNITGLTGIMDFKESGSNSHVQFEILGSSFSETFGKDIKRLATWDSVHGLNGSLKESRIENGMQGLTVKVVTLLEDPFVMVAENILGQPKRYKGFSIDVLDALAKILGFKYEIYQVADSKYGSQLPNGSWNGMIGDLINKRADLAVSAITITPERENVVDFSKRYLDYSVGILLRKPEEKINIFSLFAPFDLAVWACIAAAIPVVGVLIFLLNRLQALRSSSSQNAPPGHLSNGVGSGTLHSAIWIVYGAFVQQGGDGMVGSVALRIVMGSWWLFTLIVCSSYTANLAAYLTVSRMDHAIRSFQDLARQIDVEYGTVRDSAVYDYFRNKGTNPLEQDSTYAELWRTISKNDGMDYSVSSPSEGIRKVCVSVCGKLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4A9</accession>
    <name>A0A3B5L4A9_9TELE</name>
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        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        </location>
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    </evidence>
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        <source>
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    <sequence length="142" mass="15505" checksum="2F2DB2916007C13E" modified="2018-12-05" version="1">HTELQGECLGILLPVAHNNCSLTNDVQQDPPAILGTLQGPATITCSHSVSTFNVILWYQKPTGGSALQLVGHTLYENPTVEPEFNGRFSIKGDGSKKSELRVEKLQPEDSSIYYCAASNGRTTASCSCWDTRTEPLHSQRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4G4</accession>
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        <recommendedName>
            <fullName evidence="9">E3 ubiquitin/ISG15 ligase TRIM25-like</fullName>
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    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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        <property type="protein sequence ID" value="ENSXCOP00000005707.1"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
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    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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        <location>
            <begin position="11"/>
            <end position="54"/>
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            <begin position="145"/>
            <end position="185"/>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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            <dbReference type="PROSITE" id="PS50089"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50119"/>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005707.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="43054" checksum="BF4CAEDA881F414B" modified="2018-12-05" version="1">MEQLDRETFSCSICLDLLKDPVAIPCGHSYCMNCITNFWDEGEKNKNYHCPQCRKTFTQRPDLQKNTMLAALVEQLKKTGLQAAPADLHYAGPEDVACDSCTGRKLKAIKSCLFCLASFCEKHLQPHHDVAPFNKHKLVEPSKNLQENICSCHDEVMKMFCRTDQKCICYLCSVDEHKGHDTVSAAAERTERQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
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    <accession>A0A3B5L4Z9</accession>
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            <fullName evidence="5">Shadow of prion protein 2</fullName>
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        <name type="scientific">Xiphophorus couchianus</name>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <begin position="1"/>
            <end position="27"/>
        </location>
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            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
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            <dbReference type="Proteomes" id="UP000261380"/>
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    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="137" mass="14852" checksum="A1A9087CA3FBF9FF" modified="2018-12-05" version="1">MMGVQLKLLSFWVVMLLTAALCPGSLSKRGGSSRGHGKGDGDKASPSQGRGLSKKGLKWAGAAAAGMLGGTGTGYGLGILGKPKHGTGNHHNHKTEQHQQPYYQETQRYNNQSLWRVFVRGSGHIQLWKQRTICLRK</sequence>
</entry>
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    <accession>A0A3B5L5V3</accession>
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        <location>
            <begin position="33"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
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            <dbReference type="PROSITE" id="PS51886"/>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006197.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="194" mass="22178" checksum="23DB5E7F3FDB4C1C" modified="2018-12-05" version="1">IITIDEAKRRQSFSSCDRDSTVDALPMLIDSSDLLQDTHIEKLACRLPARVQGYLWRLVYSTEKHGTSLKTLYRNLLDVDSPVLLVVKDMDEQQIFGAFSTHPFRVSEHFYGTGETFLYSFCPEIKVYRWTGENSYFVKGNTDSLQMGGGGHLGLWLDGELYRGTTTKCATFNNQPLSTQQDFNIHSLEVWTFE</sequence>
</entry>
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    <accession>A0A3B5L7U2</accession>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    </evidence>
    <sequence length="119" mass="13593" checksum="30267E23F1B1A596" modified="2018-12-05" version="1">MLLRTAMREEGLFRLAAAASVVKRLKTCLNQGAVDHGEFSMDPHAVAGALKCYLRELPEPLMTFELYSDWFAAFRELLQKLPPENYNNLRCQQIDFHSREGNISSNSELFLTNDQNLIT</sequence>
</entry>
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    <accession>A0A3B5L7X2</accession>
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        <submittedName>
            <fullName evidence="2">Si:dkey-112a7.4</fullName>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="78" mass="8206" checksum="D7D1E24D7349AD48" modified="2018-12-05" version="1">MYGASGIPELIPHSGPPRQPGPAGQFNPGHPQADGHDGGANPQRLGQRAPKLGQIGRSKKDLDDEDLDDLMNNNGQCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAD3</accession>
    <name>A0A3B5LAD3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-2-microglobulin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-2-microglobulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027860410.1">
        <property type="nucleotide sequence ID" value="XM_028004609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAD3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006696"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006778.1">
        <property type="protein sequence ID" value="ENSXCOP00000006696.1"/>
        <property type="gene ID" value="ENSXCOG00000005183.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114136557"/>
    <dbReference type="KEGG" id="xco:114136557"/>
    <dbReference type="GeneTree" id="ENSGT00940000165013"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042612">
        <property type="term" value="C:MHC class I protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002474">
        <property type="term" value="P:antigen processing and presentation of peptide antigen via MHC class I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001005">
        <property type="entry name" value="Beta-2-microglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050160">
        <property type="entry name" value="MHC/Immunoglobulin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944:SF62">
        <property type="entry name" value="BETA-2-MICROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944">
        <property type="entry name" value="MHC CLASS II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0490">MHC I</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017230766" description="Beta-2-microglobulin" evidence="8">
        <location>
            <begin position="20"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="24"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="116" mass="13250" checksum="C81C519D5427061D" modified="2018-12-05" version="1">MKELVFLVVVLYASSAVVAKTVSPKVQIYSRNPGLYGNKNVLICHVSGFHPPEIKIDLLKNGQEINDFKQTDLAFEENWHYHLTKHANFTPVEGEHYMCRVTHVGTTTQHEWEADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAK2</accession>
    <name>A0A3B5LAK2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CCM2 scaffold protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCM2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAK2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006766"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006849.1">
        <property type="protein sequence ID" value="ENSXCOP00000006766.1"/>
        <property type="gene ID" value="ENSXCOG00000005238.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016168"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="P:anterior/posterior axis specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:cardiac jelly development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:heart contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd13516">
        <property type="entry name" value="HHD_CCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13166">
        <property type="entry name" value="PTB_CCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.20:FF:000004">
        <property type="entry name" value="Cerebral cavernous malformation 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032375">
        <property type="entry name" value="CCM2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026159">
        <property type="entry name" value="Malcavernin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21642:SF4">
        <property type="entry name" value="CEREBRAL CAVERNOUS MALFORMATIONS 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21642">
        <property type="entry name" value="CEREBRAL CAVERNOUS MALFORMATIONS PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16545">
        <property type="entry name" value="CCM2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="3">
        <location>
            <begin position="10"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="318"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="318"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="343"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="379" mass="41634" checksum="F72E89C4F17A6CEE" modified="2018-12-05" version="1">MEEDVKKVKKYLGQLTSVPGYLNPSSRTEVLQLIDNARKSHQLAGQLTSDQDAVVSLSAYNIKLVWRDGEDIILRVPIHDIAAVSYIRDDSLHLVVIKTAQESGGSPCPSSCPDLNKSQTLSSLSESGAVLVEVCCLLVLAVDNKAAAEELCLLLSQVFQIVYTESTIDFLDRAIFDGATTPTRHPSLYSDDSSSKVDVKEAFERETTYIGVYLFRELSTTAAELLQDYMTTLRTKLSSQEIQQFAALLHEYRNGSSIHEFCINLRQLYGDSRKFLLLGLRPFIPEKDSQHFENFLETIGVKDGRGIITDSFGRCKRTASSASDSTTNGNGAAGESGDSTASEEGRDASEGDEWDRMITDISNDIEALGCSMDQEAVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCB0</accession>
    <name>A0A3B5LCB0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Papilin b, proteoglycan-like sulfated glycoprotein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCB0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005509"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005572.1">
        <property type="protein sequence ID" value="ENSXCOP00000005509.1"/>
        <property type="gene ID" value="ENSXCOG00000004301.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156891"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000001">
        <property type="entry name" value="semaphorin-5A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF281">
        <property type="entry name" value="PAPILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="7">
        <location>
            <begin position="436"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="496"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="528" mass="58444" checksum="8741A8960C85D4AD" modified="2018-12-05" version="1">MIQHSSLGLSVTQPVNDYWGEFGPYGPCSRTCGTGVAMRTRKCITSRTDGGHNCVGSSKSFLTCNTHACPVGSRDFREEQCSEFDRMDFQGKRHTWVPYYGASNPCELNCVPRGQNFFYRHRLTVMDGTPCYVGRTDICVDGVCRVRFEAHLCISVHSSAPVAVSPHPRDTRTYIYRAGVYGECSATCDRGMQYRSVDCWVQDPSNPHVVDESYCITQRLQRPQSQQACNMHPCAFESVIGCQCSVTCGEGQQTREVVCVGARGEHLPDHACRGLARPASVQTCRRPACHTHISWHVTEFGLCSRSCEGGVRERKVGCFDADLNPFSEDQCGFDSRPTSVETCNAQPCNRPQSEPNVDFPMQITLDLRTAAFTDIVFLPSLGHPCVQSLYGCCPDGHTPATGPRNQGCTQDDCVRSRYGCCLDGVTPARGFGMAGCPEYQTPGVGLKFGPCDNWMVRFVYDYSTGKCKEFWYGGCHGNANNYRSMEACRRECEDVSRGPAPARRTSRRGSLGSIARARAQVSRLQRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDM3</accession>
    <name>A0A3B5LDM3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDM3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005964"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006030.1">
        <property type="protein sequence ID" value="ENSXCOP00000005964.1"/>
        <property type="gene ID" value="ENSXCOG00000004627.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155439"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050970">
        <property type="entry name" value="Cl_channel_volt-gated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720:SF10">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="155" mass="17148" checksum="BC5EED2C1074C728" modified="2018-12-05" version="1">MAALFPDGIHSDESIYLIMPGAYAVAGAAALSGAVTHTVSTSVIVCELTGQISHILPMIIAVLLANAVAQSLQPSYYDSQIRIKKLPYLPDLGWGHHEYVHRNILMKSLKQICNSTCLLKNTLYNYNKKLHLLNSLPLLQIFQTFLEQCKSPVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDP7</accession>
    <name>A0A3B5LDP7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TRAF-interacting protein with FHA domain-containing protein A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TIFA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDP7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007811"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007909.1">
        <property type="protein sequence ID" value="ENSXCOP00000007811.1"/>
        <property type="gene ID" value="ENSXCOG00000005999.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154589"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033621">
        <property type="entry name" value="TIFA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31266:SF2">
        <property type="entry name" value="TRAF-INTERACTING PROTEIN WITH FHA DOMAIN-CONTAINING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31266">
        <property type="entry name" value="TRAF-INTERACTING PROTEIN WITH FHA DOMAIN-CONTAINING PROTEIN A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="7">
        <location>
            <begin position="44"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="155" mass="17733" checksum="D45C45D4317213EF" modified="2018-12-05" version="1">METEEDLLTLLHIKFYHPQQHCKGLYNSLPLGTRSRCSADDPLRLGRDAQACTYSLVDHKVSRKQLALNAYRTPKSPDMLFSIQNLSQRGRLSVNGSSLGYLERMDLPDKTLIRFLEYEMVIIRESGEAKTSFEVDMEVLTVPPSRETMVFGHQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEX1</accession>
    <name>A0A3B5LEX1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fucolectin tachylectin-4 pentraxin-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a defensive agent. Recognizes blood group fucosylated oligosaccharides including A, B, H and Lewis B-type antigens. Does not recognize Lewis A antigen and has low affinity for monovalent haptens.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fucolectin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LEX1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009498.1">
        <property type="protein sequence ID" value="ENSXCOP00000009387.1"/>
        <property type="gene ID" value="ENSXCOG00000007069.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01060000248575"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042806">
        <property type="term" value="F:fucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010185">
        <property type="term" value="P:regulation of cellular defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001868">
        <property type="term" value="P:regulation of complement activation, lectin pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051941">
        <property type="entry name" value="BG_Antigen-Binding_Lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006585">
        <property type="entry name" value="FTP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45713">
        <property type="entry name" value="FTP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45713:SF8">
        <property type="entry name" value="SI:CH211-215K15.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22633">
        <property type="entry name" value="F5_F8_type_C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00607">
        <property type="entry name" value="FTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0430">Lectin</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017245416" description="Fucolectin tachylectin-4 pentraxin-1 domain-containing protein" evidence="10">
        <location>
            <begin position="24"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Fucolectin tachylectin-4 pentraxin-1" evidence="11">
        <location>
            <begin position="22"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Fucolectin tachylectin-4 pentraxin-1" evidence="11">
        <location>
            <begin position="175"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="316" mass="35028" checksum="85D0DBA8CBA8DB7A" modified="2018-12-05" version="1">IMRYDVLFHLLLLLPMYSGYTYQNVALRGKATQSSQYKGEQWNVFVGASNAIDGNLNADLTKGSCTHTDTENNPWWRVDLLDSYIVTQVIITNRGDCCAEQLNGVEVRIGKSLRQDGTANPLVATVSTVAAGSSYAINFTERVEGRYVIIIAPGVNRVLHVCEVEVYGYRAPTEENVAVYGKASQSTVYPGAIAYYAIDGYREGRFSQSSCSATNNDFSPWWRLDLGRTHKVFNINITNRVEVPTRINGAEIRIGDSLDNNGNNNPRCAVISTIAPGFTETFQCNGMDGRYVNVVIPGRNEYLTLCEVEVYASRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LF38</accession>
    <name>A0A3B5LF38_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027882682.1">
        <property type="nucleotide sequence ID" value="XM_028026881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LF38"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008400.1">
        <property type="protein sequence ID" value="ENSXCOP00000008301.1"/>
        <property type="gene ID" value="ENSXCOG00000006357.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114150476"/>
    <dbReference type="KEGG" id="xco:114150476"/>
    <dbReference type="CTD" id="9293"/>
    <dbReference type="GeneTree" id="ENSGT00940000163422"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000177">
        <property type="entry name" value="probable G-protein coupled receptor 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="61"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="372" mass="41662" checksum="27528FEBB155D6C0" modified="2018-12-05" version="1">MNHSELITHPTLTANSSDGDIFSGRVSNHSCPLGWGLKEGVEACILETVVIVLLTVLIIVGNLTVIFVFHCAPLLHHYTTSYFIQTMAYADLLVGLSCLVPTLSLLHYPAGVQEPITCQVFSYVISVLKSVSMACLACISVDRYLAITKPLSYNQLVTPCRLRGCIAIIWVYSSLVFLPSFFGWGKPGYHGDIFEWCAHSWPTSAIFTGFVVCFLYAPAALVVCFTYYHIFRICQQHNREISERRARFPSQEMEPGEGNGGGHHGGHGPDRRYAMVLFRITSVFYMLWLPYIIYFLLESSHVLDIPALSFITTWLAISNSFCNCVIYSLSNSVFRLGMRRLSQTMCSFSHCGADERDFGEPKPRKRANSCSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFB8</accession>
    <name>A0A3B5LFB8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 6">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFB8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009537"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009649.1">
        <property type="protein sequence ID" value="ENSXCOP00000009537.1"/>
        <property type="gene ID" value="ENSXCOG00000007263.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154514"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21063">
        <property type="entry name" value="BTHB_FKBP25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000006">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043368">
        <property type="entry name" value="FKBP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041200">
        <property type="entry name" value="FKBP3_BTHB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46493">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46493:SF1">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18410">
        <property type="entry name" value="BTHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="7">
        <location>
            <begin position="90"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="186" mass="20751" checksum="EC1267841D07964B" modified="2018-12-05" version="1">MAEPAREWSDEQLKSDDLPKKDVIKFLQDNAAHSFLNEHKLLGNIKNVAKTAKKEQLVIAYNQLFESKGPPKYTKAVLKKGDKTNFPKKGDTVSCWYTGSLEDGTVFDTNIPAAAKKKKQTKPLSFKVGMGKVIRGWDEALLTMSKGETARLEIEPEWAYGKKGVPDSKIPPNAKLIFEVELVSVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFD7</accession>
    <name>A0A3B5LFD7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000006584"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006664.1">
        <property type="protein sequence ID" value="ENSXCOP00000006584.1"/>
        <property type="gene ID" value="ENSXCOG00000005095.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158137"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000027">
        <property type="entry name" value="PH domain and leucine rich repeat protein phosphatase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055071">
        <property type="entry name" value="RA_PHLPP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF43">
        <property type="entry name" value="PH DOMAIN AND LEUCINE RICH REPEAT PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23010">
        <property type="entry name" value="RA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="5">
        <location>
            <begin position="791"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1057"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1213"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="80"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1071"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1090"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1121"/>
            <end position="1138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1318" mass="144308" checksum="B1B2230324CE05A5" modified="2018-12-05" version="1">MYSLTSVNSSTSVNSLLNRRRHRHKRNLSLGAPPGASSVEAPNPAPSASPLSTLSLDRKTFLRQKQSKQLQDDDTKSDTVDTSELIKSPSKCSSSSLTRPLTSQPSVRPDQEIPGGSEDWSEPLCTCPTPTLFIQLHGGVVRRLGDDERPLQILNEYLTNLGFKDEWRVQEEGMNPEVGCLIRFYFGKPRSVGGSERVQLSGVFNVRKGKLALPVNRWSKRQVTLSGTCLIVSSVKHAHTGKMHILPLIGGKVEEVKRHSHCLAFSSAGPQSQIYYVSYDSYAEHLRWHRMASKIASQRVNSVDLSCCSLEELPAQLFYSQDLTHLNLKNNFMSLHKGVPALTRFCKLRSLSLSNNALSEFPLVLCDITSLSELNLSGNRLSCLPAEVATMNNLQTLLLDGNIVSSLPAELGSLESLTYLGLSFNCFSSVPPVLEKLRGLEKLCLAGNRLSVLDVAGLQWLHTRYVDVRLNRLDKVTVADWEQLLHIIQLDVRDAGLQELDVSCLGKLELLRCDRNSLSFLRVSGHALKILHASHNGVPDWLCQSNRLEVLDINHNCVTELPVWLLSSGSLRKLLAGWNDMCTLPDRLESSQLEVLDLQHNHLTELPHCLFIKAQSLRYLNASANKLEDLPAASLSDDSCSSLEELYVTNNSLTDKCIPLLTGHRRLRVLHLAYNQLQTFTASKLARLELLEELDLSGNRLRAVPTTILSCRRLHMLSAHSNCINTFPEVLQLPEIKCVDLSCNELTEFTLPEVLPPKLLELDLTGNPRLNLDHKSLELLNESHGVPAVWSHGFTEASGVKNKLCVAALAQDTFCGTREALYGVFDGDKNVEVPYLLQCTMGDVLAEELHRGPKQEDYMTNTFLTMQRKLGTAGQRMGGSAALCHIRHDPVAPGEPGSCFTLKAANVGRCQAVLCRGGRALLLSNVHTVREETEYERVRQHNAVITEDNKVSGVTDSTRMMGYSFLSPSVTPRPQVSMVTLTPQDEFFLLGSRGLWDLLSPNEAIEAVRNVPDALAAAKKLATLAQSYGCSDSLSAVVIQLNITEDCCCFCEPPPPPPSPGPAAQPGLHPFTSSSDGGIQLPPTSSGTVSELSSEFSTSEMSSEVGSTASSEEPPAPPTEPLSSHLNAQGRIGARRPAAGGGGFQRQFSGALSDNGLDSEDEEPIAGVFSNGSRVEVEADVHCLLRHGSTHSNTTNAYKPVALSNELLASNLTSPSPSLVPPSSPNQEPRSATLGHRARANGSVACQGRSQDLIEEAADAPIRKQGGYFNAPAQPDPDDQLIIPPELEEEVRQIIQQQQEQMQTHTYQRPADYFVTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHB7</accession>
    <name>A0A3B5LHB7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
            <location evidence="1 9">Extracellular space</location>
            <location evidence="1 9">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHB7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009076"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009187.1">
        <property type="protein sequence ID" value="ENSXCOP00000009076.1"/>
        <property type="gene ID" value="ENSXCOG00000006921.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160329"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035284">
        <property type="term" value="P:brain segmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021587">
        <property type="term" value="P:cerebellum morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021592">
        <property type="term" value="P:fourth ventricle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030917">
        <property type="term" value="P:midbrain-hindbrain boundary development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19333">
        <property type="entry name" value="Wnt_Wnt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000001">
        <property type="entry name" value="Wnt homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009139">
        <property type="entry name" value="Wnt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF91">
        <property type="entry name" value="PROTO-ONCOGENE WNT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01841">
        <property type="entry name" value="WNT1PROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="6 9" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017308173" description="Protein Wnt" evidence="11">
        <location>
            <begin position="22"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="262"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="268"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="371" mass="41059" checksum="C15B6C4B9426ADC9" modified="2018-12-05" version="1">TMRSLALLLGVKAACILLVSSLSRTGAVNNSGRWWGIVNVASSSNLLTNSKSVQLVLDPSLALLSRRQRRLIRQNPGILHAIAAGLHTAIKECKWQFRNRRWNCPTTYSPAIFGKIVNRGCRETAFVFAITSAGVTHAVARSCSEGAIESCTCDYRRRGPGGPDWHWGGCSDNVDFGKMFSREFVDSGERGRDLRYLTNLHNNEAGRMTVSSEMRQECKCHGMSGSCTVRTCWMRLPSFRTVGDFLKERFDGASRVIYANKGSNRASHRAEPRHLEPENPAHKPPSSLDLVYFEKSPNFCSYNGKTGTLGTSGRTCNSSSPGLDGCELLCCGRGFKTQTESVTERCHCTFHWCCHVSCLNCTSTRTLHQCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHW4</accession>
    <name>A0A3B5LHW4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
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            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="Ser/Thr_kinase_AS"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
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            <begin position="247"/>
            <end position="498"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
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            <begin position="135"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
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            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
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            <begin position="135"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
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            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
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            <begin position="198"/>
            <end position="213"/>
        </location>
    </feature>
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        <location>
            <position position="276"/>
        </location>
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            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="523" mass="58843" checksum="ECDBCF0C9D77CA0E" modified="2018-12-05" version="1">MSDSVDIEEKPPAPPLRMNSSSRDSSSVNHASKPLPMAPEEKNKKARLRSIFPGGDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGEFTGIPEQWARLLQTSNITKLEQKKNPQAVIDVLKFYDSKETVNNQKYMSFTSGGNKTPTTKEEEDEDEDDEDELPPVIAPRPEHTKSIYTRSVMDPPKPPTPVKEESTPPESQVQPDNTSNTMYRHTDRQRKKSKMTDEEILERLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAIDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNSNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQNKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPERLSSVFRDFLNRCLEMDVDRRGSAKELLQHSFLKLAKPLSSLTPLIVAAKEAIKNSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LI96</accession>
    <name>A0A3B5LI96_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">diphthine methyl ester synthase</fullName>
            <ecNumber evidence="4">2.1.1.314</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 4 S-adenosyl-L-methionine = diphthine methyl ester-[translation elongation factor 2] + 4 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="EC" id="2.1.1.314"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the diphthine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.314" evidence="4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010592"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010716.1">
        <property type="protein sequence ID" value="ENSXCOP00000010592.1"/>
        <property type="gene ID" value="ENSXCOG00000008011.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010568"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141133">
        <property type="term" value="F:diphthine methyl ester synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000004">
        <property type="entry name" value="Putative diphthine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="111"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR036432-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="255" mass="28392" checksum="AF5269C4AF39B8D0" modified="2018-12-05" version="1">MLYLIGLGLGDATDITVKGLRAVKSCSRVYLEAYTSILTVGKEALEEYYGKQLILADRDLVEQGADQILKDADVTDVAFLVVGDPFATTHSDLVLRAANAGIPYRVIHNASIMNAVGCCGLQQLYNFGETVSVVFWTETWKPESFYDKICKNRAAGLHTLCLLIKVKEQSIENMMGKKIYEPPRFMTVSQAADQLIQIIERRRSEEEEELVTEDTVCVGLARLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIA7</accession>
    <name>A0A3B5LIA7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Angiopoietin-like 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIA7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009396"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009507.1">
        <property type="protein sequence ID" value="ENSXCOP00000009396.1"/>
        <property type="gene ID" value="ENSXCOG00000007155.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159478"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:triglyceride homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
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        <property type="entry name" value="Gamma-fibrinogen Carboxyl Terminal Fragment, domain 2"/>
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        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
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        <property type="entry name" value="ANGIOPOIETIN-RELATED PROTEIN 4"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR19143">
        <property type="entry name" value="FIBRINOGEN/TENASCIN/ANGIOPOEITIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
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    </dbReference>
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        <property type="entry name" value="FBG"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myosin rod fragments"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="220"/>
            <end position="444"/>
        </location>
    </feature>
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        <location>
            <begin position="114"/>
            <end position="205"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="448" mass="50905" checksum="1A64614607C9FE69" modified="2018-12-05" version="1">MKTTIAALILCLVVLMASAFPFERKGASPSTVAAKEKHVQYAARDDVNILAHGLLQLGQNLKEHVDKTKGQMRDIFTKLKAFNRTTAELGKESQRQQVEGDSLRARARDLEDREGQLLNVTAELREKVEEMQQERRTMSERMSRLEERVDGMLQGPGSRNSSDARSIQLMLDSQNRRIDDLVERIRLQQEKLDKQNVRIRTLQSQVSRGPAHSDAEEQHDSPLEMATDCHELFLRGETTSGVYPIQPTDSESFNVFCEMTADGGWTVIQRRQDGSVDFDQLWQAYEKGFGSLNGEFWLGLENIHAITKDDSYSLNIKFSDWGDDSASVRFPFELGGKETNYSLLIQEPGTTDTLESSLTTDSALALPFSTRDQDNDRKGDINCAKHLSGGWWFSNCGRSNLNGRYFQSPPPKQRHQRKQAIFWKTWRGRYYPLKSSIMMIAPATINKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIZ4</accession>
    <name>A0A3B5LIZ4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIZ4"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
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    </dbReference>
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        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
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        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="487"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="590" mass="67607" checksum="53A0BDEA0CD48BFC" modified="2018-12-05" version="1">MAAVSDHGLKRIVWKSQNWYLLVALNFCSQILILDPFTTKLLSSCCKMSDLMEERITIVEDLFKSREPVPEMKAIYFMTPTSKCVDAFIADFKSKPKYKSAYVYFTDYCPDDLFNKMKNSCARHIRVCKEINISFLPQEAQVFTCDDPGAFRRIYSPHSQDKSKTLETLADQLVTLCATLNEYPGVRYKKDNMLNAQTLAELVDNKLARHYELDDSGKKKEKTQAQLLIVERSFDPVSPILHELTYQAMAYDLIDIQNDTYRYKSKDGLDKEALLNENDMLWVRLRHNHIAEVSEQIPKMVKEIAASKKQPDGKTTISNLAQMMKKMPSFRKHVTEKTIHLQLAEDCMKHFSNNVEKLCKAEQDLAVGSDVEGTKVKDPMRTLLPVLLNPFSKHDKIRAVLLYIFSLSGTTDENLNKFIQHVKIEDEREFILNWKELGVPIITTSPSFFSRKPNRRDRSQDETYNLSRWTPVIKDVMEDAVENKLDSRDWPHQSECPAAWNGSGAVSARQRNKTSTQDDRRTGSRLIIFVIGGITYSEMRCAYEVTQAVKSCEVIVGSSHILTPISLLEDIKALSKGPMETFTIEERSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJ16</accession>
    <name>A0A3B5LJ16_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription factor E2-alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Transcription factor 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJ16"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010877"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011002.1">
        <property type="protein sequence ID" value="ENSXCOP00000010877.1"/>
        <property type="gene ID" value="ENSXCOG00000008215.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18943">
        <property type="entry name" value="bHLH_E-protein_E47-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000001">
        <property type="entry name" value="Putative transcription factor 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051098">
        <property type="entry name" value="NeuroDiff_E-box_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11793">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11793:SF7">
        <property type="entry name" value="TRANSCRIPTION FACTOR E2-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="9">
        <location>
            <begin position="374"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="121"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="455"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="197"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="460"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="478" mass="51565" checksum="196545ED78EAF444" modified="2018-12-05" version="1">MPSEIPLPSPDSLSPPSAKSNSQFYSSYERKRPAQDLTGKNADIHIFFFSSDIPTGEDFNRDTTCFPASKAGNVYQSTFYMQGQLKQYFPLESLLPGYSKRHPLPLSPQNYPLHGSEVNGAHPTSFHAGSSSFGVPSHTPPIAGTETIMANRGAVPGSSGDEIGKALASIYPSDPNSNAFPPSPSTPSGSPQAVSGCASQWTRSSGQATPSPNFEGGIQSMQSKLEDRLEEAINVLQRHASGQGGPGLAEMNSLLSAGLGIAPGFNSAALGLASRLQGLVSSHLEDSVGLPSGGGVLHHGPASVHSGSQPDGFTGRFFCSHSIKREEREDDENCSITDKSEDDRKDLKARLRTSLVSLTDENLTAEEKEQKERDRRLANNARERVRVRDINEAFRELGRMCQVHLQSDKAQTKLIILQQAVQVILSLEKQVRERNLNPKAACLKRREEEKVSGLEHQLQLGGGHPSLGGDGHNPMSHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJP8</accession>
    <name>A0A3B5LJP8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">FYVE, RhoGEF and PH domain containing 4a</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJP8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009959.1">
        <property type="protein sequence ID" value="ENSXCOP00000009841.1"/>
        <property type="gene ID" value="ENSXCOG00000007465.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155765"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046847">
        <property type="term" value="P:filopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15741">
        <property type="entry name" value="FYVE_FGD1_2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13236">
        <property type="entry name" value="PH2_FGD1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000061">
        <property type="entry name" value="FYVE, RhoGEF and PH domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000013">
        <property type="entry name" value="FYVE, RhoGEF and PH domain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035941">
        <property type="entry name" value="FGD1-4_PH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF98">
        <property type="entry name" value="FYVE, RHOGEF AND PH DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DH" evidence="15">
        <location>
            <begin position="166"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="14">
        <location>
            <begin position="381"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="17">
        <location>
            <begin position="501"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="16">
        <location>
            <begin position="502"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="14">
        <location>
            <begin position="585"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="91"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="116"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="690" mass="78608" checksum="1EAECF47A7C31BA9" modified="2018-12-05" version="1">MEDGGGKPTTTPRDRVKPSKVSDLISRFEESADNKRDGSALKQTSKVSSSSSALRVCQLTDISKIQEKFSSPAAAAQDAKRPAANGVLAQMEQDKETEEETHHSVRTEITELVNGDLERPDEVDDHSASQHSDRTGAENEGREKTTETQQKSEEGHSEQKETNEQKLFKIASELLHTEKAYVARLNLLDQQVFCTKMMEEANKGTFPLDVVKNIFSNITSIHAFHSQFLLPDLEKRMGEASTPRIGDILQKLTPFLKMYAEYVKNFDKAMELLKQWTDRSPPFKAIIQEIQSQEICGSLTLQHHMLEPVQRVPRYEMLLKDYLKKLPQNDPDRSDAESLEIIATAATHSNSAIRKSENLKKLMEIYEMLGEEEDIVHPSNEFIKEGHIMKLAARNTSTMDRYLFLFNNMLLYCVPKFSLGGPKYTVRTRIGIDGMKVVETSNEDHPHTFQVSGKERMLELQAAFRETIDIFQQKNESFKNALKDVEEKAELGKRAPRWIRDNEVTMCMKCREPFNAITRRRHHCRACGYVVCWKCSDNKAHLEYDNYKVNKVCKDCYSILTGEGVTEGRRKGILEQIEAAQFTDSSIMSGFLQHSEKGGKLWQRVWCVIPEKECLVLYLYGAPQFLQDVKALCTIPLLGYTVEDADTPASFRLSQSKFVHTFAAESEELKQRWLKVIRAAVTGEMPARPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LK21</accession>
    <name>A0A3B5LK21_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3-O-(beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl)-L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = 3-O-(beta-D-GalNAc-(1-&gt;4)-beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23464"/>
            <dbReference type="Rhea" id="RHEA-COMP:12573"/>
            <dbReference type="Rhea" id="RHEA-COMP:12575"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="ChEBI" id="CHEBI:132093"/>
            <dbReference type="ChEBI" id="CHEBI:132105"/>
            <dbReference type="EC" id="2.4.1.174"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus</location>
            <location evidence="1 13">Golgi stack membrane</location>
            <topology evidence="1 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the chondroitin N-acetylgalactosaminyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="RefSeq" id="XP_027889590.1">
        <property type="nucleotide sequence ID" value="XM_028033789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LK21"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011252"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011383.1">
        <property type="protein sequence ID" value="ENSXCOP00000011252.1"/>
        <property type="gene ID" value="ENSXCOG00000008500.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114154568"/>
    <dbReference type="KEGG" id="xco:114154568"/>
    <dbReference type="GeneTree" id="ENSGT01050000244968"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047238">
        <property type="term" value="F:glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047237">
        <property type="term" value="F:glucuronylgalactosylproteoglycan 4-beta-N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050650">
        <property type="term" value="P:chondroitin sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000059">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008428">
        <property type="entry name" value="Chond_GalNAc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051227">
        <property type="entry name" value="CS_glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12369:SF19">
        <property type="entry name" value="CHONDROITIN SULFATE N-ACETYLGALACTOSAMINYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12369">
        <property type="entry name" value="CHONDROITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05679">
        <property type="entry name" value="CHGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="70"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="529" mass="60711" checksum="3DE16713BEE4A64E" modified="2018-12-05" version="1">MLRRWLLALMTRIGLITSGCCCFLLLFYLLGCKPSSHSKHPSLLWPGGATSKEGYLALLQEREDSHRHYISSLTKQIAELKVALEERTQQLQESLETAKSKGILPQGLESLHNTPTKSDLKEFFRSQLNQAEVSSGVELPNEYALIPFDTFTLQTVYQLETGLTKHPAERPSRRDRRDELVGTVETALHVLNGPQQHADDTRRKRTYSPSDFIQGLTRTERTRGTVYELVFKGDGPQNFAQLVLFRPFGPLMKVKSDGVSTHGIIINIIVPLSGRVDAFEQFMSNFRDVCVRQDGRVHLTVVYFGRDQIDQVKAVLDQITRETRFRSFTLIQMNEKFSRGRGLDVGARAWRQRQNVLLFFCDVDVHFTPDFLTSCRLNADAGKKVYYPVLFSQYNPSIIYSNHTHIPSIKQQLVIKNENGFWRDFGFGMTCQYRSDFINIGGFDRSIRGWGLEDVHLYRKYLHSKLMVVRAPSRGLFHLWHEKSCADELPPDKYKMCMQTKAMSEASHGQLGALFFKREINSQKQSQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKK2</accession>
    <name>A0A3B5LKK2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein argonaute-1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the argonaute family. Ago subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKK2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010121"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010240.1">
        <property type="protein sequence ID" value="ENSXCOP00000010121.1"/>
        <property type="gene ID" value="ENSXCOG00000007676.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155256"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016442">
        <property type="term" value="C:RISC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04657">
        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.260.10:FF:000001">
        <property type="entry name" value="Protein argonaute-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000001">
        <property type="entry name" value="Protein argonaute-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000005">
        <property type="entry name" value="Protein argonaute-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032472">
        <property type="entry name" value="ArgoL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032473">
        <property type="entry name" value="Argonaute_Mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045246">
        <property type="entry name" value="Piwi_ago-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16488">
        <property type="entry name" value="ArgoL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16487">
        <property type="entry name" value="ArgoMid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01163">
        <property type="entry name" value="DUF1785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="4" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="PAZ" evidence="9">
        <location>
            <begin position="57"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="10">
        <location>
            <begin position="345"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="687" mass="78199" checksum="2F75148398842DA1" modified="2018-12-05" version="1">RYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYEADPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEVIQDLASMVRELLIQFYKSTRYKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKEYQPGITYIVVQKRHHTRLFCADRNERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADEFQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHHDTLRTMYFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKP6</accession>
    <name>A0A3B5LKP6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ras homolog gene family, member Gd</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKP6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011492"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011624.1">
        <property type="protein sequence ID" value="ENSXCOP00000011492.1"/>
        <property type="gene ID" value="ENSXCOG00000008675.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163331"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12453" checksum="D11973F5C2157183" modified="2018-12-05" version="1">MQTIKCVMVGDGEVGKTFLLVTYTTSAFKEKHLSALFDNYTGDISVDGHTVTLTLWDTAGREGYEQLRALAYAQADVFVVCFSIGSPSSYANVRLKWKPEVSHSERWKIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKV9</accession>
    <name>A0A3B5LKV9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MALT paracaspase 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKV9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012718"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012872.1">
        <property type="protein sequence ID" value="ENSXCOP00000012718.1"/>
        <property type="gene ID" value="ENSXCOG00000009596.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018044"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08783">
        <property type="entry name" value="Death_MALT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000004">
        <property type="entry name" value="Mucosa-associated lymphoid tissue lymphoma translocation protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052039">
        <property type="entry name" value="Caspase-related_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037940">
        <property type="entry name" value="MALT1_Death"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041077">
        <property type="entry name" value="MALT1_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033540">
        <property type="entry name" value="MALT1_IG-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22576">
        <property type="entry name" value="MUCOSA ASSOCIATED LYMPHOID TISSUE LYMPHOMA TRANSLOCATION PROTEIN 1/PARACASPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22576:SF40">
        <property type="entry name" value="MUCOSA-ASSOCIATED LYMPHOID TISSUE LYMPHOMA TRANSLOCATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18703">
        <property type="entry name" value="MALT1_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="105"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="201"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p20" evidence="2">
        <location>
            <begin position="290"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="681"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="681"/>
            <end position="690"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="782" mass="88037" checksum="E6B44393528CB0C1" modified="2018-12-05" version="1">MSDSLERSTKINSLKESIVKKLCEVLDKSSNKGWRKLGEIVSSDRRFKVSSDDMEMCSLRVLEVEGSPSLTLLRLMGERGCTTGHLMDYLQTLGNSDALQCLKPPALQILIQPQSVALICGHNLRLSCHAVGKSPVQYQWFKSREEVPNSFSPELLISPVQLKDAGFYICRVNCGDACEFSQWAQVDVLNVTIYQSIDGRLKLAIHPQSQRLHVGETLQLECGAVGRPIPRYQWHRNGVPIPKATKRKLMIPQLMQDHHGRYRCEISSSTERMWTNEVDVVIGMLRFFATDKVALLIGNLSYQNHPQLKAPMVDVYDLTNLLRQLNFKVVSLLDLTESEMRNAVDEFLLLLHKGVYGLLYYAGHGYENYGNSFMVPVDAPNPYHSANCLCVQSILKLMQEKETGLNVFLLDMCRKRNIHDESTPNIVLRVTANIVFGYATCQDAEAFELSSTGFTNGVFVKFLKKRLLDDEKITVVLDRVAEDMGQFDATKGKQALEIRSSLSERRALTDPILPGDSEDLVHAHSRQWAKAHELPESMSLNFDCGAQIKLGFAAEFSNVLVIYTHIVKKPEDMSFCQAHDLDVDPKEMNRETPEETGIYLLSSSLPQHCLYTRLSSLQKLREELVFTVCLQGTFAAMDDDHPIFWTKSVNIGKPLIARLDLHRAVRRNSCLQTCMMPHSPSQSPCHSPGPEHQHHLHHGSHQDYSRLSPQHPYLHISEHAQGAAGGCGFSCYDQLSQCDFDRTYESDGLLSSPGKLSIPIEATEDINELQAVFISSLHLQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LL38</accession>
    <name>A0A3B5LL38_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Zinc finger, SWIM-type containing 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LL38"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010276"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010399.1">
        <property type="protein sequence ID" value="ENSXCOP00000010276.1"/>
        <property type="gene ID" value="ENSXCOG00000007791.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158362"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031462">
        <property type="term" value="C:Cul2-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048370">
        <property type="entry name" value="ZSWIM4-8_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22619">
        <property type="entry name" value="ZINC FINGER SWIM DOMAIN CONTAINING PROTEIN 4, 5, 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22619:SF2">
        <property type="entry name" value="ZINC FINGER SWIM DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21055">
        <property type="entry name" value="ZSWIM4-8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZSWIM4-8 C-terminal" evidence="4">
        <location>
            <begin position="120"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="306" mass="34308" checksum="6463A975CCA80E30" modified="2018-12-05" version="1">MIKSSCLFSFPCHFLKNWQSLRALVSILQSWYTLFTPTEATSIVAATVMSHNTILRLSLDYPQREELASCARTLALQCAMKDPQNCALSALTLCEKDHIAFETAYQIVIDAASTGMTYSQLFTIARYMEHRGYPLRSFKLASLAMTHLNLAYNQDTHPAINDVLWACALSHSLGKNELAAIIPLVVKSVHCATVLSDILRRCTMTAPGLAGIPGRRNSGKLMSTDKAPLRQLLDATISAYINTTHSRLTHISPRHYGEFIEFLSKARETFLLAQDGLIQFAQFIDNLKQIYKGKKKLMMLVRERFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LL44</accession>
    <name>A0A3B5LL44_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LL44"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011390"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011522.1">
        <property type="protein sequence ID" value="ENSXCOP00000011390.1"/>
        <property type="gene ID" value="ENSXCOG00000008595.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008160"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002177">
        <property type="term" value="C:manchette"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097225">
        <property type="term" value="C:sperm midpiece"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007288">
        <property type="term" value="P:sperm axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010441">
        <property type="entry name" value="CH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056199">
        <property type="entry name" value="SPEF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054517">
        <property type="entry name" value="SPEF2_D5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052634">
        <property type="entry name" value="Sperm_flagellar-bone_growth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14919">
        <property type="entry name" value="KPL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14919:SF0">
        <property type="entry name" value="SPERM FLAGELLAR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06294">
        <property type="entry name" value="CH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24082">
        <property type="entry name" value="SPEF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22946">
        <property type="entry name" value="SPEF2_D5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="2">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="792"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1277" mass="146663" checksum="A1E5A6341A2E9F8E" modified="2018-12-05" version="1">SKVYDTIIAVCFLDARNFCKDFSNGYLFGEILHKYQMQEDFNMFLKSDSSDAKTNNFSRLEPTIKRLGVSFNKSTAQDLMQKKHGVATNLLYQLYVALEEKKPAGTSRVGKLPSLQKRESETQSYVRALNKCVRYFAVREVRDERKLEKSEKVKNMENKLYTFIFIIFSPSDQSIPADSSLSPVIQDIEVPSDKPDKVTIQSSTKYVQGIRQQLKENAVAREERQKRVDRFLLEQYKALIYPQQEQILTLEDQMVRRLTRQTQQEQRLAEQLMQIRRQKEVILENRLFREEQYKRQREKDFQEALDKEHAKLARAEEIEKEIEFCKMVAAERAHEKHMKHYTICSETLGQIVDMATTVGEYRSITENLVPEKKYKEWKELFIRGVPFYESQKDIEPEILFLAALNPSELKKEEVLNNLDYDEYTNMIGVWTWPKATEETKLPPSINDILAYAIQRIKTIAHPTVAEPATTVPLFSIKSCIKGKVCQCKSKCMAKIAEGLKSLKKRDFRIKACVLGKASSGKTTCLAKIAEGIKFYLKMCMASIAASVERELRKGNTIPNELIVDIVVDAISQVSAQSGWILDGFPTNIGQAQLLEKALGGFVEEEHGTEINTYELVPNPEQLKPPTPSPPVLDVALLLDISDECVIRRASIDKGTIMIAAFQDTWPELEKWYGEKQNILVNINADVEERELYNKVESSLCEVMKEGDSRCGDSSASEEQVPEYLCSHWKDVCNSYVNSMKHVMQQLRLQLTLIDRHLFNIRERYTHFLSCPDLKQELVSQWQKVFNSVPDDMREDEETKAELHLRLDELQERLWDITDKRKEEDQQERSAFMCDGWLEEQSALLINHYSMIIQAEMNRFRETLCTLAVYYWGMHKHLPLRTSPKDFSITLLKETGAEDQDKDQCNEYCGCNKMGKYLGVLNAARYFKHVLCTECFKFSVSSIDQLGEVVRYHIEASSKLQYELVMVCKFYLNGDCLLVSGVVFPPRPPSMEKPNRSILTVAQLESLFNHLFNMAPSGLCVHQRLIGLLLSLLFFPLMLIDWRRFLLSATLPWPLPSLSQLLYLLKQFKEADTDDTGYINEDQYLQVELWFPGQSVQKVPEDPLEPLPYDRSANLQKFFFQMFADHSSSPPLLDYVSMLQYFAANPNPKQGFIRALSVVLGKHLQQPLLSLLVNRYLHLNVQNLSGATNTATVFTGKLFCNNFEDLSCSPCSRLLNLFSSFREILFSKDLKPSDTFICDWLQCCVLIGRSGQASVAGRQCLCSSSMSGLCSSIKVSAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LL59</accession>
    <name>A0A3B5LL59_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Chromobox homolog 5 (HP1 alpha homolog, Drosophila)</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027856898.1">
        <property type="nucleotide sequence ID" value="XM_028001097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LL59"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011667"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011800.1">
        <property type="protein sequence ID" value="ENSXCOP00000011667.1"/>
        <property type="gene ID" value="ENSXCOG00000008798.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114134441"/>
    <dbReference type="KEGG" id="xco:114134441"/>
    <dbReference type="CTD" id="23468"/>
    <dbReference type="GeneTree" id="ENSGT00940000158801"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd18651">
        <property type="entry name" value="CD_HP1alpha_Cbx5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18655">
        <property type="entry name" value="CSD_HP1alpha_Cbx5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000007">
        <property type="entry name" value="Chromobox protein homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000009">
        <property type="entry name" value="chromobox protein homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017984">
        <property type="entry name" value="Chromo_dom_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008251">
        <property type="entry name" value="Chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051219">
        <property type="entry name" value="Heterochromatin_chromo-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22812">
        <property type="entry name" value="CHROMOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01393">
        <property type="entry name" value="Chromo_shadow"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00504">
        <property type="entry name" value="CHROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00300">
        <property type="entry name" value="ChSh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Chromo" evidence="8">
        <location>
            <begin position="18"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="8">
        <location>
            <begin position="132"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="70"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="199" mass="22387" checksum="6980713DFD08D7E6" modified="2018-12-05" version="1">MGKKSRDEESSSSDEEEYVVEKVLDRRVVKGRVEFYLKWKGYSDKHNTWEPEKNLDCPELISEFMKTYKKGSTGSATPSSGASKSTTGPSGRSKESGSSKKRSSDDDEEGSSKPKKKKEDEILVARGFERGLEPEKIIGATDSCGDLMFLMKWKDSDEADLVLAKEANHKCPQIVIAFYEERLTWHEDSDKKEKDAVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LND9</accession>
    <name>A0A3B5LND9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LND9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013608"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013775.1">
        <property type="protein sequence ID" value="ENSXCOP00000013608.1"/>
        <property type="gene ID" value="ENSXCOG00000010304.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161924"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07723">
        <property type="entry name" value="hydroxyacylglutathione_hydrolase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000019">
        <property type="entry name" value="Hydroxyacylglutathione hydrolase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01374">
        <property type="entry name" value="Glyoxalase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035680">
        <property type="entry name" value="Clx_II_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032282">
        <property type="entry name" value="HAGH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017782">
        <property type="entry name" value="Hydroxyacylglutathione_Hdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03413">
        <property type="entry name" value="GSH_gloB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935">
        <property type="entry name" value="BETA LACTAMASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935:SF77">
        <property type="entry name" value="HYDROXYACYLGLUTATHIONE HYDROLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16123">
        <property type="entry name" value="HAGH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005457">
        <property type="entry name" value="Glx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="6">
        <location>
            <begin position="11"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="290" mass="33116" checksum="D8928E4633D60358" modified="2018-12-05" version="1">MKVKVISILEDNYMYLVIEEQSKQALAVDPAVPHRLLEIVKREGLFLVAVLTTHYHWDHARGNEALVNEVPSLKVYGADDRIKGLTDKVTDSQELKFNSINVKCLFTPCHTSGDMCYYVWEDECTDAPAVFTGDTLFIGGCGRFLEGTAEQMHHNLTKVIASLPQETKVFCGHENTIKNLKFALLVEPENEKVKEMLSWARARDDDDKPTVPSTLKEEFDYNPFLRLSEKAVQKFTEKMEPVEVMRVLRKEMDKFKKPKERLPPQAMLALQWGLLMSSKDHLSPAIKDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPV8</accession>
    <name>A0A3B5LPV8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Olfactory receptor C family, v1</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPV8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014320.1">
        <property type="protein sequence ID" value="ENSXCOP00000014143.1"/>
        <property type="gene ID" value="ENSXCOG00000010705.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ANF_lig-bd_rcpt"/>
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        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
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        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF504">
        <property type="entry name" value="EXTRACELLULAR CALCIUM-SENSING RECEPTOR ISOFORM X2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017467964" evidence="13">
        <location>
            <begin position="18"/>
            <end position="863"/>
        </location>
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            <begin position="613"/>
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            <begin position="651"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="711"/>
            <end position="729"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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            <end position="811"/>
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            <begin position="823"/>
            <end position="846"/>
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            <begin position="613"/>
            <end position="861"/>
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            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="863" mass="96220" checksum="908B7E714A5ADAE4" modified="2018-12-05" version="1">MPLLALFSLIFYVSVLSFTPPPVSRYTSTAPTAAEDCVLRNSFQPAFVAEGDFIMGGIFPLHYNQEMPDQNYTYTPPPVKFSSVPATFLSLTTLFNMRAWACVCVCVFASLLSHRFDPRAFRWVQTMRLAVEEINESTQLLLNHTLGYKIFDSCAYPLTGQRAALAVLNGLSEADSPMCSGASPMLAVIGESGSAQSIVVSRLLRPFRIPMISYFSSCACFTDRRQYPTFFRVIPNDDYQAIAQLLQHFNWTWVGLIRGDHEYGRFAVQGLLRELQGSSVCHLFSYYAKQLQSDLKRFILKYEGTEGEMTPFLRDYMKQNITGIQWVASEAWVTASVFTGREFYPYLGGTIGFGVRKGHIAQLSDYLQTVNPQQYPNNVLVGELWEALYGCSPFPSSANPFPVCSGQEVLLKQHSAYMNTSSPRVAYNVYKAVYAVAHSLHNLLLCQPGNGPFENDSCAQSNKILPWQYYLQEVKFDMAGEEVNFDLKGDSVPYYDIINWQRGTTGSIEFVNVGLFDGTNLIVKKAGFANRTHIVPVKVPGSVCSPSCPPGSRKAVRRGEPVCCFDCVPCDSGKISNQTNSVECIFCPEDFWSNQDRTNCIPKKVEFLAYDALGIPLTVISVVGASLTIGVFVVFFYHRSTAVVRVDNSELSFFILLALTLCFLCSLVFIGEPTVWSCMLRHTTLVVLAAFTATRPGDNIMKWFGPKQQRAIIFSCTLVQVVICAAWLIDAPPYPIRNTKYERSKIILECNVGSSLAFWCVLGYIGLQACLCFVLAFLARKLPGNFNEAKYITFSMLIFSAVWLAFVPVYVSAPGNYAGAVESFAILASSFGLLFCLFAPKCYIILLKPEKNTKQHLMGKEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQG2</accession>
    <name>A0A3B5LQG2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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            <taxon>Poeciliinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="AlphaFoldDB" id="A0A3B5LQG2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011784.1">
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        <property type="gene ID" value="ENSXCOG00000008799.1"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="193" mass="21291" checksum="B77F3A25DEFFF652" modified="2018-12-05" version="1">MFPPDPPFEGATVQSQSNLFVFGNFIAFILNSFTHLDIDDLLRWHLPSLHKCTSLCCSVSCMVVAAGLSCAAAVIIFGYSSLTLTYGEEDEDVFHQHKSAKVTFVLHRLVKGANATIILTCIISLVLSSLIGYVGFRSLPCCGCYDSRTGLVRTGSPPPLGVRLKELIMSWYNVSPKVMLLCLWKLLKQLLRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQW4</accession>
    <name>A0A3B5LQW4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
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    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQW4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013437"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013603.1">
        <property type="protein sequence ID" value="ENSXCOP00000013437.1"/>
        <property type="gene ID" value="ENSXCOG00000010189.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
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        <property type="entry name" value="ZN346_ZMAT4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46144:SF1">
        <property type="entry name" value="U1-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46144">
        <property type="entry name" value="ZINC FINGER PROTEIN 385B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="U1-type" evidence="8">
        <location>
            <begin position="13"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="16"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="U1-type" evidence="8">
        <location>
            <begin position="65"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="U1-type" evidence="8">
        <location>
            <begin position="137"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="216" mass="24973" checksum="186C0168E935FB4D" modified="2018-12-05" version="1">MKSSDEELLQGLLTDYYCHICSSNLMFEANRVAHYEGKQHSQKVKTYLNAMRADLGRGCQNMSHDKNSFCELCYVVFSSHVVAKSHYEGKIHAKNLRKRSLLVSGKYFLTQQCIIHQLSPRIPLTPTRTRPRRTTRNPNKYCALCVASFNNPQMALQHYNGRKHQKKMARQELLKAHGKDAQQGIARQWIYVMCGVSTNSLKMYQAHMQGNKHQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSV1</accession>
    <name>A0A3B5LSV1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Suppressor of cytokine signaling 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Substrate-recognition component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex, also named CRL5 complex), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as DAB1 and IRS1. Specifically recognizes and binds phosphorylated proteins via its SH2 domain, promoting their ubiquitination. The ECS(SOCS7) complex acts as a key regulator of reelin signaling by mediating ubiquitination and degradation of phosphorylated DAB1 in the cortical plate of the developing cerebral cortex, thereby regulating neuron positioning during cortex development. Functions in insulin signaling and glucose homeostasis through IRS1 ubiquitination and subsequent proteasomal degradation. Also inhibits prolactin, growth hormone and leptin signaling by preventing STAT3 and STAT5 activation, sequestering them in the cytoplasm and reducing their binding to DNA.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Substrate-recognition component of the ECS(SOCS7) complex, composed of SOCS7, CUL5, ELOB, ELOC and RNF7/RBX2. Interacts, via the third proline-rich region, with the second SH3 domain of the adapter protein NCK1. Also interacts with GRB2, INSR, PLCG1, SORBS3/vinexin, and phosphorylated STAT3 and STAT5. Interacts with SEPT6. Interacts with phosphorylated IRS4 and PIK3R1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSV1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015088"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015277.1">
        <property type="protein sequence ID" value="ENSXCOP00000015088.1"/>
        <property type="gene ID" value="ENSXCOG00000011435.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156314"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046935">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd10388">
        <property type="entry name" value="SH2_SOCS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03741">
        <property type="entry name" value="SOCS_SOCS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000029">
        <property type="entry name" value="Suppressor of cytokine signaling 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035866">
        <property type="entry name" value="SOCS7_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037346">
        <property type="entry name" value="SOCS7_SOCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF5">
        <property type="entry name" value="SUPPRESSOR OF CYTOKINE SIGNALING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 16" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="8" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SH2" evidence="18">
        <location>
            <begin position="560"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="19">
        <location>
            <begin position="664"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="47"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="331"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="59"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="331"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="722" mass="78964" checksum="54CCBBE53D41CB4C" modified="2018-12-05" version="1">MRLVSAAEEDRLEGENGNLPTGGVVVGLGNDVLAYSGVKSALDISRGPTETAGLEHPSSLLNKGQPSSDTTAAPDSGGTEGRVPQSSMDPQGFDFVPSPGLRPQLLVFSNIMRNGDGILDLDRRDALELGPAVNNNNNTPSPGDVERHHDLLDQRPSTLQSSVDSSAESPGSAEMCHRHRFITNPHNWPLLSDRSGPLPGGDSRGQEDAVFELARRFGELGVGPRMLFKGAELPQCSCQGAQGSVDGQAEPRDDPTETSDALLVLEGLGSRGINGLGIEECPEVGLDDENGHGEFLLKRKREIIHKMSGTFSISSFQVELRTQIEKMGLPTTQAGSEGSAQVLAKSSTPAQNSPWTTTPNPSQASTPRRGKRCTSAPRTPTYRGAGGEKTFKVPGKSKKNSLKTRLSKLFRTKSCSGSNHILDKRPSVSYSVSSAMSLVDMEGGSGAEQDADSHREPRLTRAHSAFSPASLSASLSTFTVSLVDVDISLRGSKTSHPPTPPRRSLSLLDAFFRALPHSNPLPSDAAPPTRRAAPPVLCALRRSEASNFTASLRELEKCGWYWGPMNWEDAEVKLKGKLDGSFLVRDSSDPRYILSLSFRSQGVTHHTRMEHYRGTFSLWCHPKFEDRCHSVVEFIERAIMHSKNGKFLYFLRSRVPGLPPTPVQLLYPVSRFSNVKSLQHLCRFCIRQIVRIDHIQELPLPRSLISYLSKFYYYDPEEEMYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSX0</accession>
    <name>A0A3B5LSX0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSX0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014092.1">
        <property type="protein sequence ID" value="ENSXCOP00000013920.1"/>
        <property type="gene ID" value="ENSXCOG00000010542.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000174523"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784:SF3">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 4 MEMBER K-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="36"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="141" mass="16542" checksum="24426EC300681638" modified="2018-12-05" version="1">ISYTSSRFMFVLQFHSQITGTVASMHLPVNLILYMKTWLEAQSYCREKHTDLISGTKQLQDEEVRKLMNSSSTYPHIGLFRDTWRWSDGNSFSFRHWNNDFNYPQSNSGQCAMTVFNDGGKWRNVSCKIYLDKYNLICSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSY7</accession>
    <name>A0A3B5LSY7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSY7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010919"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011044.1">
        <property type="protein sequence ID" value="ENSXCOP00000010919.1"/>
        <property type="gene ID" value="ENSXCOG00000008231.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161224"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF38">
        <property type="entry name" value="SODIUM-DEPENDENT DOPAMINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="240"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="269"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="449"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="480"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="524"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="557"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="424"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="622" mass="69894" checksum="71F289CB55E70C62" modified="2018-12-05" version="1">MLCVGNPGRRLKLAQQMQTHFHVSVYVCMISLVPQVELILVKEQNGVQFTSTTLVAPAQTIPSGEEERETWGKKIDFLLSVIGFAVDLANVWRFPYLCYKNGGGAFLVPYLFFMVIAGMPLFYMELALGQYNREGAAGVWKICPIFKGVGFTVILISLYVGFYYNVIISWALFYLFSSFTRELPWVHCNNTWNSPNCSDWADNSSIGDIYKATPAQEYFERAVLHIQDSNGIGDLGRPRWQLTSCLAVVIILLYFSLWKGVKTSGKVVWITATMPYVVLTVLLLRGVTLPGAIDGIKAYLSVDFLRLCDAKVWIEAATQICFSLGVGFGVLIAFSSYNKFSNNCYRDAIITSSINSLTSFFSGFVVFSFLGYMSHKHNVALDKVARDGAGLVFVIYPEAIATLPGSSVWAVIFFIMLLTLGLDSAMGGMESVITGLIDEFKFLHKHRELFTLFIVVSTFLISLFCVTNGGMYVFTLLDHFAAGTSILFGVLIEAIGIAWFYGVDRFSDDIKEMIGHRPGRYWRLCWKFVSPCFLLFMVVISFATFNPPNYGSYMFPQWANLVGWCLAMSSMTMVPLYAIYKLCTLPGRFCDRLAYAITPETEHHLVDNGEVRQFTLHHWLVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LT94</accession>
    <name>A0A3B5LT94_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Rho GDP-dissociation inhibitor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Rho-GDI alpha</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer. Interacts with FER. Interacts with PLXNB3. Forms a heterodimer with RAC1. Interacts with RHOA, the affinity is increased by three orders of magnitude when RHOA is prenylated. Interacts with PSMD10; the interaction increases ARHGDIA association with RHOA, leading to ARHGDIA-mediated inactivation of RHOA and ROCK and prolonged AKT activation. Interacts with KANK2; the interaction is direct and may regulate the interaction of ARHGDIA with RHOA, RAC1 and CDC42. Interacts with RHOC. Interacts with CDC42. Interacts with NGFR (via death domain); NGFR binding decreases the affinity for RHOA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rho GDI family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LT94"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015228"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015419.1">
        <property type="protein sequence ID" value="ENSXCOP00000015228.1"/>
        <property type="gene ID" value="ENSXCOG00000011540.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006233"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005094">
        <property type="term" value="F:Rho GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.50.30:FF:000004">
        <property type="entry name" value="Rho GDP-dissociation inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.30">
        <property type="entry name" value="Coagulation Factor XIII, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000406">
        <property type="entry name" value="Rho_GDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024792">
        <property type="entry name" value="RhoGDI_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980">
        <property type="entry name" value="RHO GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980:SF9">
        <property type="entry name" value="RHO GDP-DISSOCIATION INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02115">
        <property type="entry name" value="Rho_GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00492">
        <property type="entry name" value="RHOGDI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="200" mass="22876" checksum="F0F3B8488D26C7B7" modified="2018-12-05" version="1">MAEDEVTPEQLAAIAAENEEPEPVNYKPPAQKSVKEIQELDKDDESLRKYKEALLVVSSDPNAPNVQVTRMSLICESAPNPLVLDLQGDLEAFKKQAFVLKEGVEYKIKISFKVNKEIVSGLKYVQQTFRKGMKIDKSDYMVGSYGPRPNEYDFLTTMEEAPKGMLARGNYVIKSKFTDDDKHDHLSWEWNLNIKKDWND</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTC7</accession>
    <name>A0A3B5LTC7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CSC1/OSCA1-like cytosolic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTC7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014362"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014546.1">
        <property type="protein sequence ID" value="ENSXCOP00000014362.1"/>
        <property type="gene ID" value="ENSXCOG00000010893.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157084"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="1">
        <location>
            <begin position="3"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="82" mass="9671" checksum="FD6E5D742CC41325" modified="2025-10-08" version="2">MEAQLRDEYRKEREKVNKKPLGMAFVTFQNEATTAKILKDFNACKCQGCYCRREPKSSQFSSRLHTSNWTVTYAPDPQNVYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVC2</accession>
    <name>A0A3B5LVC2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Aquaporin 9b</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>H2O(in) = H2O(out)</text>
            <dbReference type="Rhea" id="RHEA:29667"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>glycerol(in) = glycerol(out)</text>
            <dbReference type="Rhea" id="RHEA:29675"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>urea(in) = urea(out)</text>
            <dbReference type="Rhea" id="RHEA:32799"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVC2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015097"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015286.1">
        <property type="protein sequence ID" value="ENSXCOP00000015097.1"/>
        <property type="gene ID" value="ENSXCOG00000011439.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164919"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015254">
        <property type="term" value="F:glycerol channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050363">
        <property type="entry name" value="MIP/Aquaporin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829">
        <property type="entry name" value="AQUAPORIN OR AQUAGLYCEROPORIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829:SF26">
        <property type="entry name" value="AQUAPORIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="272" mass="29844" checksum="96F628F4BA25B64C" modified="2018-12-05" version="1">MEEESRRKMKEKFGLRRDMSKEFLAEFLGIFVLIKIKFSSTFRTVLYFTESFMTFTVGVSHVNPAVSLAMVILGKLPLKKFPVYVVAQFLGAFAGSCAVFGLYYGELKCRKCGKTKITGENATANIFASYPAKHLSVLNGFVDQVIATGALVLCILAITDRKNIGAPKGMEPLCIGLTIMAIGVSMGLNCGYPINPARDLGPRLFTAVAGWGMEVFRAGGCWWWIPVAGPMVGGAVGAGIYFLFIDLHQPEPQKQDDNNVQDKYEIISRNTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVP5</accession>
    <name>A0A3B5LVP5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Suppressor of fused homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the SUFU family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVP5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016033"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016234.1">
        <property type="protein sequence ID" value="ENSXCOP00000016033.1"/>
        <property type="gene ID" value="ENSXCOG00000012114.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009747"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045879">
        <property type="term" value="P:negative regulation of smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.230:FF:000001">
        <property type="entry name" value="Suppressor of fused homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.230">
        <property type="entry name" value="Sufu, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020941">
        <property type="entry name" value="SUFU-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024314">
        <property type="entry name" value="SUFU_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038489">
        <property type="entry name" value="SUFU_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037181">
        <property type="entry name" value="SUFU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007768">
        <property type="entry name" value="Suppressor_of_fused"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016591">
        <property type="entry name" value="Suppressor_of_fused_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10928">
        <property type="entry name" value="SUPPRESSOR OF FUSED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10928:SF2">
        <property type="entry name" value="SUPPRESSOR OF FUSED HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05076">
        <property type="entry name" value="SUFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12470">
        <property type="entry name" value="SUFU_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011844">
        <property type="entry name" value="Suppressor_of_fused_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103359">
        <property type="entry name" value="Suppressor of Fused, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Suppressor of fused-like" evidence="13">
        <location>
            <begin position="26"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Suppressor of fused C-terminal" evidence="14">
        <location>
            <begin position="216"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="287"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="295"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="444" mass="49850" checksum="E2FD8EF3129FB93D" modified="2018-12-05" version="1">MDVSCQIAGPQNRLFPCLTARKVLGGPDPLDYISMYRNMGCAAQDVQEHWHYVSFGLSDLYGDNRVHEFTGTDGPSGFGFELTFRLKREAGETAPPTWPAELMQGLARYVFQSENTFCSGDHVSWHSPLDNSESRIQHMLLTEDPQMHPIQTPFGAVSFLQIVGVCAEELQASQQWNGQGILELMRGIKVAGGPWLITDMRRGETIFEIDPHLQQERVDQGIETEGSNLSGVSAKCVWDDLSRPPDDEEDSRSICIGSQPRRLSNKDTEQIRETLRKGLEFNSKAALPPISSQKQNHDRAQIRKDSLESESSAAIVPHELVRTRQLESVHLKFNQESGTLLPLCLRGRLLHGRHFTYKSINGDTAITFVSTGVEGAFATEEHPYAAHGPWLQILLTEEFVEQMLGDLHELGSRDESKLPKEYSWPEKKLKISILPDSVFDNPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LW69</accession>
    <name>A0A3B5LW69_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Target of myb1 like 2 membrane trafficking protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TOM1L2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LW69"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013561"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013728.1">
        <property type="protein sequence ID" value="ENSXCOP00000013561.1"/>
        <property type="gene ID" value="ENSXCOG00000010271.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156940"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14238">
        <property type="entry name" value="GAT_TM1L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.160:FF:000001">
        <property type="entry name" value="TOM1-like protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13856:SF31">
        <property type="entry name" value="TOM1-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13856">
        <property type="entry name" value="VHS DOMAIN CONTAINING PROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GAT" evidence="2">
        <location>
            <begin position="76"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="293" mass="32060" checksum="44E079CDEB62CBFC" modified="2018-12-05" version="1">MADLDALSPIHTPQRLAEDFCSVFPLESQGTPEVDPGMMKYLAPNASAPGNPKPTQPTPTATQASSIPSPVTATPEQIARLRSELDIVRGNIKVMSEMLTELVPGQEDASDLELLQELNRTCRAMQQRVVELISRVSNEEVTEELLHVNDDLNNIFLRYERYERYRSGRAAQNNGMLAEPTEDNLIDLGPGSPAVVTPRVVSSPTSNVSSDSVSGTLTALSGHNKDDFDMFAQTRSSSLAEQRKNVKYEDPHALGGLASALDVRQQNTSGVSTSKRPLCVRFTMYGAWRGIFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWC2</accession>
    <name>A0A3B5LWC2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UBX domain-containing protein 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">UBX domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">May negatively regulate the ATPase activity of VCP, an ATP-driven segregase that associates with different cofactors to control a wide variety of cellular processes. As a cofactor of VCP, it may play a role in the transport of CAV1 to lysosomes for degradation. It may also play a role in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins. Together with VCP and other cofactors, it may play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with VCP through the PUB domain (via C-terminus) and VIM motif (via N-terminus); the interaction is direct. Forms a ternary complex with CAV1 and VCP. Interacts with SYVN1. Interacts with HERPUD1. Interacts with VCPKMT. May interact with DERL1. Interacts with PLAA, VCP and YOD1; may form a complex involved in macroautophagy. Interacts with LMAN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWC2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012139"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012277.1">
        <property type="protein sequence ID" value="ENSXCOP00000012139.1"/>
        <property type="gene ID" value="ENSXCOG00000009175.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157273"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10460">
        <property type="entry name" value="PUB_UBXD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16119">
        <property type="entry name" value="UBX_UBXN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000185">
        <property type="entry name" value="UBX domain-containing protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036339">
        <property type="entry name" value="PUB-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018997">
        <property type="entry name" value="PUB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042774">
        <property type="entry name" value="UBXN6_PUB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23153:SF38">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23153">
        <property type="entry name" value="UBX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09409">
        <property type="entry name" value="PUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00580">
        <property type="entry name" value="PUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143503">
        <property type="entry name" value="PUG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBX" evidence="9">
        <location>
            <begin position="310"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="14"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00059509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00065525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00070523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00075815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="420" mass="47514" checksum="75FE51AED7DC20C3" modified="2018-12-05" version="1">MKKLFDDFKKDIKFKSAGPGKKLTEDASSSSKQSRHAPSEGAQMAGAAALARIEQKQRPKVHTSQDAIRNQVKRELEAEAAALAEKENAGSKVKDAACLSVSGVYFTCPLTGATLTKSQREVHIKEAILMRFEEDDIEASVMMVHTFNKDREKVKAAVDIISKYVDNICKNPSEEKYRKIKVSNKVFQEKVSPVEGSREFLQAVGFISVMLPEEEEFLVLPEQSDDALELMKERRDRLQRGEPVRAQLDRQPQAFRPSPNAQRFELPPEFYNLSAEELKKEQQQRTELVEKNAMLRTKAMREREEQRERRKYNYALLRVRLPDGNLLQATFYAWDRLPALFEFVRESLVDGWQPFELIAPGGQKLQESEEVTLVECNLVPAALLTFAWDAAVQADIAAAGGKSSAALLKPELLERIQTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYY9</accession>
    <name>A0A3B5LYY9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sorbitol dehydrogenase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Polyol dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYY9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014566"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014751.1">
        <property type="protein sequence ID" value="ENSXCOP00000014566.1"/>
        <property type="gene ID" value="ENSXCOG00000011050.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074781"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003939">
        <property type="term" value="F:L-iditol 2-dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006062">
        <property type="term" value="P:sorbitol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05285">
        <property type="entry name" value="sorbitol_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000068">
        <property type="entry name" value="Sorbitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045306">
        <property type="entry name" value="SDH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF9">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="12">
        <location>
            <begin position="8"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="320" mass="34377" checksum="B61C3B25537B369E" modified="2018-12-05" version="1">MHSVGICGSDVHYWQHGQIGDFVLKKPMVLGHEAAGRVVKVGSAVSHLKPGDRVAIEPGVPRDMDEFFKRGQYNLSPTIFFCATPPDHGNLCRYYTHNANFCYKLPDNVTFEEGALIEPLSVGIHACRRAGVTLGSTVLICGAGPIGLVSLIVAKAMGASKVIITDLFAERLGVAKELGADFQLTVKKDDGAQQLAKKVEEILGAQPQITIECTGVESCIQTALYATCSGGVVMLVGLGAAMTTVPLVNAAVREVDIRGVFRYCNTWPMAIAMLSSGKVNVKPLVTHRFPLEQAVQAFETTHQGLGIKVMLKCDKNDQNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZN8</accession>
    <name>A0A3B5LZN8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Wings apart-like protein homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">WAPL cohesin release factor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with the cohesin complex throughout the cell cycle; interacts with both chromatin-bound and soluble pools of the complex. Interacts with RAD21; the interaction is direct. Interacts with PDS5A; the interaction is direct, cohesin-dependent and competitive with CDCA5/SORORIN. Interacts (via FGF motifs) with PDS5B; the interaction is direct. Interacts with a SMC1 protein (SMC1A or SMC1B) and SMC3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WAPL family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZN8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016445"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016652.1">
        <property type="protein sequence ID" value="ENSXCOP00000016445.1"/>
        <property type="gene ID" value="ENSXCOG00000012409.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015768"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001054">
        <property type="entry name" value="WAPL cohesin release factor a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000085">
        <property type="entry name" value="Wings apart-like protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039874">
        <property type="entry name" value="WAPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022771">
        <property type="entry name" value="WAPL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012502">
        <property type="entry name" value="WAPL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22100">
        <property type="entry name" value="WINGS APART-LIKE PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22100:SF13">
        <property type="entry name" value="WINGS APART-LIKE PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07814">
        <property type="entry name" value="WAPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51271">
        <property type="entry name" value="WAPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WAPL" evidence="19">
        <location>
            <begin position="535"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="51"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="250"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="334"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="216"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="276"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="438"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00064348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1095" mass="121521" checksum="D642FDE1ACEAD28E" modified="2018-12-05" version="1">MTSRFGKTYNRKGGEANSKFEEVFSNKKPTLTTKWGETTYKAQLGGKKPFLNPGATEVFKRPKLEDSDSEDPFGFDSDEESKTVTSENVSEMKIKEEPVPKTVTTESRVTAAVVTSAQASASITMSEEDVKDNQPWYKSALADSQKPVYVTCVSETVLFDNKNLSLSQMPVSSFSNLDAAMSVNVPGGMHNIQPACSYDGSLLEEMKSEDQELSTEPPPEPVDNIPPSPFTLRASNCKKYQRPHRSHIALAEFSDSDSDKPIDPSSAQDKSNSVLSASASGTAASANTAAKPAGRGGGRVRDYTVLHPSCLSVCNVTIQDSMDRSIDELVAQPTPADLGEAGQMKKKSDTQRPKSTRFRPIQTKPKKTLAQTKLEFFGFEYKDDEGEDASSEGATAGKSSYKIKYFGFDDLSESDSDDENSQAKEKKVKRAAAALAALSSSVDSPHTSDSQDSQASSNTGEFHYFGQTFIFFFSVSLSVHFLLLLQPVLSSGSKDSNSHKDDGLFKAPPPPPKVIKSETIPTRLNQEIVTALKCRKEDKELYTVVQHVKHFNDVVEFGENQEFTDDFEYLETGLKSSQPLNTRCLSIISLATRCAMPSFRMHLRARGKVAQVFKMLSDAPQHPNLALCTAALMYILSRDRLNMDLDRACLELMIKLLELDQDYSGDQDQLTAKEVAKVKEKIRKLCETVHNKHLDLENITTGHLAMEALLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVENLSQEDDKEKLVESLWGAERCLRVLESVTVQNPENQGYLIAYKDSQLIVSSARALRYCEDMIQRYSRALNNSSLSSSSGVTLPHCSFSNVGKAVEDCMRAVIGVLLNLTHDNEWGSTKTGEQEQLIVTALNCVLRVPRYIPQEQRFDVRVLGLGLLINLVEYSSRNRHCLVDMEYTVDDTCLEDSLIFFRQLTSVISPPMLQLFLERERAAILAEAKTDDLISEAPKPALDQSGEWQETSGEIQWVASETNDSQPEKKEEEDEELDLNKALQHAGKHMEDSIVASYTALLLGCLCQGSQINVTTVRQHLPKGDFSIMTEMLKKFLSFMNLTCAMGTTGQKSISRVIDYLEHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZU1</accession>
    <name>A0A3B5LZU1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZU1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016505"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016712.1">
        <property type="protein sequence ID" value="ENSXCOP00000016505.1"/>
        <property type="gene ID" value="ENSXCOG00000012458.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161979"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001249">
        <property type="entry name" value="CTCF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001840">
        <property type="entry name" value="Paternally-expressed gene 3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003287">
        <property type="entry name" value="Zgc:113343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000096">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 18 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000417">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000839">
        <property type="entry name" value="Zinc finger protein 691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
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        <property type="entry name" value="Snail/Krueppel_Znf"/>
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    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF53">
        <property type="entry name" value="CHORION TRANSCRIPTION FACTOR CF2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="206"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="234"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="262"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="290"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="346"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="374" mass="42899" checksum="1F6BED7EEC309BC7" modified="2018-12-05" version="1">MFATDVKQMLMVKEAPEDHRSVAELHDPKPQQIKEEQEEVCISLGAEQLKGEEDIDAIRFPVSATPIKNEGDKQSILLSRNLYKDEIKDRDLPKEIDEEESIKIENCGDGSIFLKTEDTEDDDVNEFTSELDSDMTHSEMGSSTTTGVKLSCEYCAKTFSGKYTLNTHERIHTGQKPFCCDLCGQRFNQKSTLNRHMRNHTGQKPFCCDFCEQKFSQKSHLIRHMRNHTGQKPFCCDFCGQRFSQKSHLSRHMRNHNGEKPFGCDLCGHKFGEKSNLNIHMRIHTGQKPFCCDICGQNFAAKSNLITHTRSHTGQKPFYCDVCGQKFSRKSHLNTHMRIHTGQKPFCCDLCGQRFNQKSNLNTHMRIHTKGKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2W4</accession>
    <name>A0A3B5M2W4_9TELE</name>
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        <recommendedName>
            <fullName evidence="1">H15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M2W4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017670"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017894.1">
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:nucleosome"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="1">
        <location>
            <begin position="18"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="116" mass="12331" checksum="F847A14ED196314A" modified="2018-12-05" version="1">MAEVAPSAVVIPADVPQKPPPKSKKGASIYKLIVAIVAESKERKGMSLTALKKALAVKGVNVAKGNKRINNAVTKLVTGGTLSRTKGTGACGSFKIIQVYQQQAKCKCKDKYIYKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M377</accession>
    <name>A0A3B5M377_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
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            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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            <taxon>Poeciliinae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSXCOP00000018598.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="Butyrophylin_SPRY"/>
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        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
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        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="4">
        <location>
            <begin position="7"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="202" mass="22729" checksum="94A616A781EC4E73" modified="2018-12-05" version="1">MVTEVDVLLSEPEPKSRAGFLKYSCEITLDPNTVNTELGLSEGRRKVTLMNQHQSYSSHPDRFTNWWQVLSRESLTGRCYWEVKRSGTCVYISVAYKNISRAGCGNECEFGCNDQSWALECSQNSFSFGHNNIWTSISGPVSSRVGVYLDHRAGILSFYSITEAMTLLHRVQTRFTEPLLAGVWVGTGSSLLQLKTRDQAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3Q8</accession>
    <name>A0A3B5M3Q8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Coiled-coil-helix-coiled-coil-helix domain containing 3a</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion inner membrane</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3Q8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017995"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018220.1">
        <property type="protein sequence ID" value="ENSXCOP00000017995.1"/>
        <property type="gene ID" value="ENSXCOG00000013554.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000903"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007964">
        <property type="entry name" value="MIC19/MIC25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052632">
        <property type="entry name" value="MICOS_subunit_Mic19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21588">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC19 ISOFORM X1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="1" id="KW-0519">Myristate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="209"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="300" mass="33498" checksum="E99461AFCA47E58E" modified="2018-12-05" version="1">MGANNSTRRVSFESDENENITVVKGIRVSYRVDLLKETGVTYLIHSPSQAVEPLPSHVPKSESLSSPPIFKPITILPASLLPSGDSLPRQSADSLPTAPLPEFTASCEPVAPPPQPTAEPLSPASALRKKITDELYKDLAQKRIKTEQDMQAWYDAEKARASAQAQADVQSKVQEEISRILSVERALTQDSLQQAIIRERISSEDEKLRAQLLVSAKQLEAKEADLKKQDAFYREQVARLEERSEQFYKVTKENYNKAVDQVNAKFNSCNFRNYSKIKGTLINMSISISKSLKCLLHLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3W7</accession>
    <name>A0A3B5M3W7_9TELE</name>
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        <recommendedName>
            <fullName evidence="1">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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    </dbReference>
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        <property type="entry name" value="GTPase activation domain, GAP"/>
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    </dbReference>
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        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="45"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="205" mass="23047" checksum="CCAF3D77D62272F3" modified="2018-12-05" version="1">MGAGAMTICVSKTAVLVREDMKRMVQIPMLRSRAVAAQSTKVFGVSLLELREHGLVEDGVPLLVRRMVEHLSKHALHQEGLFRVNGNVRAVETLKLRLESGGDEAELLSESDLCTVSSLLKRYLRDLPEGLVGSTVQQKLIQHYEDRGGDVSWLVIRDFLQQLPDVHQNLLRYLCNFLTLVDKNHEENRMTAQNLATVFGPSVFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5U8</accession>
    <name>A0A3B5M5U8_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">Cysteine-rich DPF motif domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cysteine-rich DPF motif" evidence="3">
        <location>
            <begin position="13"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="117" mass="13119" checksum="F836F0B89EF7BC11" modified="2018-12-05" version="1">MEQTATETPRNVFSCQLCGLSTSYTYYGQKPPNTRAIVLLEECFVAKDPFSPDKEKFLVLGSACSLCNMCVCVGPDCSLFYTKRFCMQCVNKHLDQFPHQIRSELAKKKQSSKSGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M736</accession>
    <name>A0A3B5M736_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Atherinomorphae</taxon>
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            <authorList>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <dbReference type="Ensembl" id="ENSXCOT00000017386.1">
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        <property type="gene ID" value="ENSXCOG00000012942.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017378294" description="Peptidase S1 domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="4">
        <location>
            <begin position="32"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="71"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="99" mass="10218" checksum="4EAF77C524BF3288" modified="2018-12-05" version="1">MRGFSPVSIIVGLNCLGVVCGQAPLASRISGGVSVASAGEWPWMASLQKNGQHVCGGTLVSERDVLTNANCFSGYQPSSPPSPPTSPMPQPPSPRQPVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7S4</accession>
    <name>A0A3B5M7S4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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            <taxon>Poeciliinae</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7S4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017371"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017592.1">
        <property type="protein sequence ID" value="ENSXCOP00000017371.1"/>
        <property type="gene ID" value="ENSXCOG00000013087.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1112">
        <property type="entry name" value="COL_CUTICLE_N DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="313"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="395"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="462"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="579" mass="59477" checksum="6D1CEE87816F5215" modified="2018-12-05" version="1">HLETRDLRSAIGAESGSKPDQSRTEPLSWLCLGLGGQCPWFPWRQRLPWTPGTEWIPRTVVKVKLGIKVIQLLVQKARLENLEFRGLQEHKDVRGPKALLGTLAQWEPALGLTGPEGFEGRPGFPGNKGNPGGSGKPGERGPKGQDGGQGCAGPKGPKGTTVVIVLPVKPYNTRIMFLIQIAVFANLLQIMDNLCPPAGHKGCAGPPGELIPRPEKGPRGPQGCPGDPGFSGPRGERNRRLCESLHVGQTLLGAISMETRWQRSDFHPALGVGLILCSDLFSINFVFSTGNKGEAGCTGNTGYPGPNGPPGCRGPQGPRGPQGKQGPPGPPGTKGHQGYFGFPGLNGELRGCKGHKGLRGCPGEPEMLSPLGVAGPPGPRGYHGLRGPPGRDGVDGPPGRDGNPGNRGDTGDPGCSGPKGRHGYQGPPGPPGMRGDSGLLGIPGEQGPRGHPGRPGPKGRPGVNGSGGPKGQKGRILSNGPPCSPVRGPSGPPGPPGASGDRGQRGEIGLLGRNGEELGLKGIKGDPGADGAMGPQGQEGSLGQMGEPGQPGENGDLGEKGKTSHLQLGRINTAGCWQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M848</accession>
    <name>A0A3B5M848_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Catenin (cadherin-associated protein), delta 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the beta-catenin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M848"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015949"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016148.1">
        <property type="protein sequence ID" value="ENSXCOP00000015949.1"/>
        <property type="gene ID" value="ENSXCOG00000012044.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156045"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003401">
        <property type="term" value="P:axis elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042074">
        <property type="term" value="P:cell migration involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008078">
        <property type="term" value="P:mesodermal cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048920">
        <property type="term" value="P:posterior lateral line neuromast primordium migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000007">
        <property type="entry name" value="ARVCF, delta catenin family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028435">
        <property type="entry name" value="Plakophilin/d_Catenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372:SF6">
        <property type="entry name" value="CATENIN DELTA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372">
        <property type="entry name" value="PLAKOPHILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ARM" evidence="10">
        <location>
            <begin position="383"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="10">
        <location>
            <begin position="426"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="10">
        <location>
            <begin position="690"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="486"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="836"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="891"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="194"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="838"/>
            <end position="851"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="921" mass="101951" checksum="C5ECF99BDF50CCC0" modified="2018-12-05" version="1">MEQCASTASLLASVREQERQFEMLSRALEEERRSCAGTLPRPLPNMQNGRIPCDADLERLTLNEGYINGTHHVRIRPLMPEARDNEHHIPFLSMDNYIRFSNIEVKKVVKTTTTRTVIPSVSDTLSVDGGGSLVGGYASPMVYRQGPGGAVPMEYPTHTVPRNYHYSPAGGYDDYRGGPPSEPYSSLSRGARMDDRYRPIHPDGYRTLDPSYRAPSRNQLDPYAAQPQVGRLGSAMELSSIPRFVADPYGLEDDQRSIGYDEPDYGMGHPMHYNTMPLNHAFPHGPPPRRTGSYEGALDGAGEVYYYGAGAPLAKGERGSVASLDTLSMRKGPPASGWRQPELPEVIAMLNYRLDPVRSNAAAYLQHLTFKNDKVKSDVRRLKGIPALVSMLDNPNREVHFAACGALKNISFGKDADNKIAIKNCDGVPALIRLLRKTRDQDLTEIITGTLWNLSSQDSVKMEIVDNALHALCDEVMVPHSGWERTTNGAGGGEENGKPRTPEWDTALTNTAGCLRNVSSERIEARRKLRECSGLVDSLMYIVQSQINCKELSNKLTENCICLLRNLSYHVHKEIPNFEAYDESKQANLGPAPSSQRAGCFSSRKGKGLLFLGKKDEEAPEERISIPPRATPAKGYELLFQPEVVRIYTSMLKESHNLIVLEASAGAIQNLCAGRWTHGRYIRALMREEKGLVVVSELLDNTNDRVVRAMSGAIRNLAIDPHNKKLLGKYAVPYLVGDLPGGGQNQPVKALSEETVVSILSTLHELLGSDVEAAKTLRASQGIERLVLTNKDSNRSDREVRGAGLVLQSVWAHKELRRTLEKDGWRKTDFMVNLNPPKSDDNRLNGRHDDSTLPLIDKGGKRDEEMIPLNEFGPDAYSTLDQRGRKNTLDKTLDAADQDSVQGGMYGERQASLPLLDSYDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAM9</accession>
    <name>A0A3B5MAM9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tubulin folding cofactor B</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TBCB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027855015.1">
        <property type="nucleotide sequence ID" value="XM_027999214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAM9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016764"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016981.1">
        <property type="protein sequence ID" value="ENSXCOP00000016764.1"/>
        <property type="gene ID" value="ENSXCOG00000012643.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114133373"/>
    <dbReference type="KEGG" id="xco:114133373"/>
    <dbReference type="CTD" id="1155"/>
    <dbReference type="GeneTree" id="ENSGT00940000156119"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043014">
        <property type="term" value="F:alpha-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01789">
        <property type="entry name" value="Ubl_TBCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000013">
        <property type="entry name" value="Tubulin-folding cofactor B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045172">
        <property type="entry name" value="TBCB_Ubl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF85">
        <property type="entry name" value="TUBULIN-FOLDING COFACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14560">
        <property type="entry name" value="Ubiquitin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAP-Gly" evidence="5">
        <location>
            <begin position="185"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="246" mass="27837" checksum="2E7978CBF3C91819" modified="2018-12-05" version="1">MDGEVTVVTNPYVNVRITSSLSSFENRRRFNKRITIAELKMLLEMNVGVPASSMDLEIFSTTDRFLQKIDNDEALFGSYPVDDECRIHVIDRSGGQTSELFDDSKVEKFELSDEAYEQRKESVRSFLKKQNLGRFNEEEAAKKKAELTIREEQQKAAAEAISVGSRCKVEVPGQPTKLGTVMYVGTTEFKSGHWVGVKYDEPVGKHNGTVQGKQYFECQDKYGAFVKPLNMTVGDFPEEDYGLDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MB27</accession>
    <name>A0A3B5MB27_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Mitotic spindle assembly checkpoint protein MAD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Mitotic arrest deficient 1-like protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
            <location evidence="4">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MAD1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MB27"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021448"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021710.1">
        <property type="protein sequence ID" value="ENSXCOP00000021448.1"/>
        <property type="gene ID" value="ENSXCOG00000016055.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001316"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990706">
        <property type="term" value="C:MAD1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990728">
        <property type="term" value="C:mitotic spindle assembly checkpoint MAD1-MAD2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000051">
        <property type="entry name" value="mitotic spindle assembly checkpoint protein MAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.457.60:FF:000002">
        <property type="entry name" value="Mitotic spindle assembly checkpoint protein MAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008672">
        <property type="entry name" value="Mad1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23168:SF0">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23168">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD1 MITOTIC ARREST DEFICIENT-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05557">
        <property type="entry name" value="MAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75704">
        <property type="entry name" value="Mitotic arrest deficient-like 1, Mad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="10" id="KW-0132">Cell division</keyword>
    <keyword evidence="19" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="15 23" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="94"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00053509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00073985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00075803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="313" mass="36767" checksum="C4B24D5020B2B6E7" modified="2018-12-05" version="1">MDLEDDTTILTTLKSFNTFISRHEHPQRPSEQSGSGNLQPHYKRSMEFLEAAERVHSQHRYLQLDQEKKQMELSHKRARFELEKAASESARNLEVQDKDEIRDLRQKIEELEKDRQRLEEQNNILEMRLERHNLQGDYDPVKTRVIHLKLNPTAEAKQQHRQEAGAVQEELTRLRELVRSYQEGGVASQDDSSIHTPGFNLRKQMESSELRNQRLKEVFQKKIQEFRTVCYVLTGYQIDITTENQYRLTSVYAEHMQDSLLFKKGSNGSMHLMETEFSKTLGEMVTLHLHHQKSIPAFLSAVTLDLFSRQTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MB74</accession>
    <name>A0A3B5MB74_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Rho-associated protein kinase</fullName>
            <ecNumber evidence="21">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="34 35">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Protein kinase which is a key regulator of actin cytoskeleton and cell polarity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 21">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="21">Activated by RHOA binding. Inhibited by Y-27632.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 21">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="21"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021498"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021761.1">
        <property type="protein sequence ID" value="ENSXCOP00000021498.1"/>
        <property type="gene ID" value="ENSXCOG00000016089.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234517"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072518">
        <property type="term" value="F:Rho-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:1901888">
        <property type="term" value="P:regulation of cell junction assembly"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01242">
        <property type="entry name" value="PH_ROCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22250">
        <property type="entry name" value="ROCK_SBD"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.10.510.10:FF:000047">
        <property type="entry name" value="Rho-associated protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000036">
        <property type="entry name" value="Rho-associated protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.730:FF:000001">
        <property type="entry name" value="rho-associated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000033">
        <property type="entry name" value="Rho-associated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000072">
        <property type="entry name" value="Rho-associated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001759">
        <property type="entry name" value="Rho-associated, coiled-coil-containing protein kinase 2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.730">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057529">
        <property type="entry name" value="MRCK/ROCK_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
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    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
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    <dbReference type="InterPro" id="IPR020684">
        <property type="entry name" value="ROCK1/ROCK2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015008">
        <property type="entry name" value="ROCK_Rho-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF73">
        <property type="entry name" value="RHO-ASSOCIATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25346">
        <property type="entry name" value="PH_MRCK"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08912">
        <property type="entry name" value="Rho_Binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037568">
        <property type="entry name" value="Rho_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103652">
        <property type="entry name" value="G protein-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51859">
        <property type="entry name" value="RHO_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 21 25" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18 24 26" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="20 21" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="17 21" id="KW-0460">Magnesium</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 21 25" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 21" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Protein kinase" evidence="29">
        <location>
            <begin position="76"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="31">
        <location>
            <begin position="341"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="33">
        <location>
            <begin position="468"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="RhoBD" evidence="32">
        <location>
            <begin position="948"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="28">
        <location>
            <begin position="1120"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="30">
        <location>
            <begin position="1230"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="768"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1015"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1313"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="26">
        <location>
            <begin position="414"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="26">
        <location>
            <begin position="937"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="768"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="1026"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="1323"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="22">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23 25">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRNR" id="PIRNR037568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR037568-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR037568-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS51859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1356" mass="157606" checksum="574348FF4F148594" modified="2018-12-05" version="1">MSAGDAMESRFEKIDAMLQDPKSEINTDCLLDGLDALVYDLDFPALRKNKSIDNFLNRYKKTISKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFFAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDGIHSMGFIHRDVKPDNMLLDKAGHLKLADFGTCMKMNEDGMVQCDTAVGTPDYISPEVLKSQGGNGNYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNALTFPDDSDISNDAKNLICAFLTDREVRLGRNGVDEIKRHPFFKNDQWTWDNIRETAAPVVPELSSDIDTSNFDDIEEDRGEEETFPIPKAFVGNQLPFVGFTYYSNQQYTSDKRSSSTKEDKSHLENLQKQIYQLEEQLHREMQQKDELEQKCRASNTKIEKIMKELDEEANLRKSAEASVSLLEKDKIMLQHRFTEYQRKADQEGEKRRNLENEVSNLKEQLEDMRKISQNSQASNDKIVQLQKQLEEANDLLRAESDTAARLRKSHTEMTKSMSQLETLNRELQERSRAADGEKAQLEKELLLLQSMLESERRNYSEGSEEMRELQARLAGLQEDNKNLKINLSKVEGERKHAQERSNYLEKEKNSLEIDLNYKLKTLQQRLEQEQTEHRVTRAQLTDKYESIEEAKSAAMIGMPQKMSEETGSRIRAEGRVVEVEKQCSMLEFDLKQSVQKMEQLMKQKERLEDEVKGLHTHLDQESSKRVAAQNDLKSRTQDVDRLRCSEKQLKQEINTALENKRSLEFQLAQLTKQYRGNEGQMRELQDQLEAEQYFSTLYKTQVRELKEEIEERNRQVQDAQKKVHDLISERDSLSAQLDLTVTKAESEQLARALQEEQYFELSQESKKATARHKQEIGEREATIAQLEESNKTLTKDVEHLSKNNTELNEKLHLQEEEYLSQKEEIMASVKGNYEKVLQTERTLKTQAVNKLAEIMNRKDMKLDQKKKGSTAELRKKEKENRKLQQELNQEKEKFNHMAIKYQKEVNEMQAQLAEESTYRNELQMQLASKESDIEQLREKLCDLQQRVENSSVTSLQTDETDSNTAESRLEGWLSIPNRANIKRYGWKKQYVVVSSKKILFYNDEQDKEQSNPSMVLDIDKLFHVRPVTQGDVYRAETEEIPRIFQILYANEGECRKEADMEPVPQGEKTNCLAHKGHEFIPTLYHFPSNCEACAKPLWHVFKPPPALECRRCHVKCHKDHLDKKEDVIAPCKVNYDVTSARDMLLLAVTQEEQKKWIGHLGKKIPKTPPSTFSRVSPRSMSTRSGPNQSFRKNPKSNTAKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBQ6</accession>
    <name>A0A3B5MBQ6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Rhomboid-related protein 1</fullName>
            <ecNumber evidence="4 13">3.4.21.105</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11 13">May be involved in regulated intramembrane proteolysis and the subsequent release of functional polypeptides from their membrane anchors.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="4 13"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBQ6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017129"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017349.1">
        <property type="protein sequence ID" value="ENSXCOP00000017129.1"/>
        <property type="gene ID" value="ENSXCOG00000012916.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160651"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000006">
        <property type="entry name" value="rhomboid-related protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017213">
        <property type="entry name" value="Peptidase_S54_rhomboid_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051739">
        <property type="entry name" value="Rhomboid_IM_Serine_Proteases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840:SF4">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037470">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="16">
        <location>
            <begin position="105"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="14">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037470-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="300" mass="34121" checksum="7C03B3E70057100F" modified="2018-12-05" version="1">MSSKRSSSFRRAIANGRRTLQREILLDETGLGLYKRFVRYVAYEILPCETDRRWYFHQNRLCPPPVFIAIIVFMCYGIMLNKWVLQTYQLDFMKSPLVYHPGHRTQVWRFFSYMFMHVGLEQLGFNALLQLMIGVPLEMVHGILRISLLYMAGVLAGSLTVSITDMRAPVVGGSGGVYALCSAHLANVVMNWAGMRCPYKLLRMILALVCMSSEVGRAVWLRFSPPVPSSGPQPSFMAHLSGAVVGISMGLLILRSYEESLQKQCSWWVIVFSFITFLLFAIFWNIFAYELLGVQIPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MC32</accession>
    <name>A0A3B5MC32_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acyl-CoA thioesterase 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ACOT9</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MC32"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021095"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021354.1">
        <property type="protein sequence ID" value="ENSXCOP00000021095.1"/>
        <property type="gene ID" value="ENSXCOG00000015781.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005330"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03442">
        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000012">
        <property type="entry name" value="Acyl-coenzyme A thioesterase 9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655:SF0">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="6">
        <location>
            <begin position="39"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="178" mass="20301" checksum="EBBEE66655EB1B57" modified="2018-12-05" version="1">VAPTNEEQKIIHDLFLNTLDANTVSFRSRVLPPNSVWMEDAKLKGLEICHPQQRNIFNRIFGGFLMRKAYELGWANACSFGGCRPSLVAVDDILFQKPVEIGSLLMLSSQVCYTQGKYIQVRVHSEVFDPLTRQHSTTNIFHYTFASDRDVPSVIPQTYGESMLYLDGKRHFNQTLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MC99</accession>
    <name>A0A3B5MC99_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TNIK</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MC99"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021165"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021424.1">
        <property type="protein sequence ID" value="ENSXCOP00000021165.1"/>
        <property type="gene ID" value="ENSXCOG00000015826.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183196"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000003">
        <property type="entry name" value="TRAF2 and NCK-interacting protein kinase isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000006">
        <property type="entry name" value="TRAF2 and NCK-interacting protein kinase isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051700">
        <property type="entry name" value="STE20_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47096">
        <property type="entry name" value="MISSHAPEN LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47096:SF1">
        <property type="entry name" value="MISSHAPEN LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="24"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="298"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="386"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="308"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="533" mass="62427" checksum="D77D35031A27EF2F" modified="2018-12-05" version="1">RNSAVPAEPPSGLDMSAGDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKAEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNSLKEEWIAYICREILRGLTHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKVGACWSLSEGALCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKSHSQRPSTEQLLKHPYIRDLPNERQVRIQLKDHIDRTKKRRGERDETEYEYSGSEEEDEERDIGEPSSIINIPGESTLRRDFLRLQLANKERSELIRRQQLEQQQNEEHKRQLLAERQKRIEEQKEQRRRLEEQQRRERELRKQQERDQRRRYEEMEQLRREEERRHAEREQEYKRKQIEEQRQAERLQRQLQQERAYLVSLQQQQEPPRPPQDKKQLYHYKDQAPSSNDKPAWAKEVTCRGRRGPGALHYEAGTGNRGVQGNCISMQMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCJ2</accession>
    <name>A0A3B5MCJ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCJ2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017635.1">
        <property type="protein sequence ID" value="ENSXCOP00000017414.1"/>
        <property type="gene ID" value="ENSXCOG00000013130.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183048"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF885">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="132"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
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            <begin position="46"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="302" mass="34399" checksum="0114C64D6D73050D" modified="2018-12-05" version="1">MSFLIFKVMNVSQVSYFTLSAYFDIGLFKYVVFLVIMCVYMFIIGSNVLLIAVICVNRSLHEPMFLFLCSLFVNQLVNLLVHSVSVPLCFLQIFCLYMYVNVEFYNLAVMSYDRYLAICHPLHYSTQMSLSMVVKLVVLSWCLPFVVIVGMIWLNVSSALCGDVIDRVFCNNYYIALLSCIGATASNVYGLVYTFSVLFSLAALILYTYVKILQVCFSGSKETRQKAVSTCTPHLVSLINFSFGSFFAVLQSRFDMSRVPNVIRIILSLYFVTCQPLLNPLLYGLKLNKIQITCRTLFFGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCQ0</accession>
    <name>A0A3B5MCQ0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cleavage stimulation factor, 3' pre-RNA, subunit 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCQ0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022028"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022295.1">
        <property type="protein sequence ID" value="ENSXCOP00000022028.1"/>
        <property type="gene ID" value="ENSXCOG00000016436.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158987"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12671">
        <property type="entry name" value="RRM_CSTF2_CSTF2T"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cleavage stimulation factor subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cleavage stimulation factor subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000061">
        <property type="entry name" value="cleavage stimulation factor subunit 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.70">
        <property type="entry name" value="Transcription termination and cleavage factor, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025742">
        <property type="entry name" value="CSTF2_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026896">
        <property type="entry name" value="CSTF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038192">
        <property type="entry name" value="CSTF_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735:SF2">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14327">
        <property type="entry name" value="CSTF2_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14304">
        <property type="entry name" value="CSTF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="29"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="508" mass="54576" checksum="A3E1EB78D56E8CD4" modified="2018-12-05" version="1">MANVAAATVAAAAAAAAANRDPAVDRSLRSVFVGNIPYEATEEQLKDIFSEVGLVVSFRLVYDRETGKPKGYGFCEYQDQETALSAMRNLNGREFSGRALRVDNAASEKNKEELKSLGTGTPIIESPFGDSVSPEEAPESISRAVASLPPEQMFELMKQMKLCVQNSPQEARNMLLQNPQLAYALLQAQVVMRIVDPEIALVRIIPLSLYQPVMHVNGAPQMMQPSNMGVPNMPGPGPAPGPGPIGPPGNRYSFLILLTRGHSKDCIASMWSRPGGIPPRGLLGDGPNDPRGGTLLSVTGEVIDPNRGYMAAPPPHQAPPVHIPQMGGGPPEMRGPPMPEPRTMMVDPRGPPMMEPRGPPMEPRGMSRRKTFEFQHHILLKLVTCFPSGRDPRAMDARGPVAGQRVPMVPGMQGPVPHAMGPNVPPPARPVTGRKSGLHSGSGEGGFINGAADVSCRRRLTPSRLFQAALIMQVLQLTPEQIAMLPPEQRQSILILKEQIQKTASGAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEN7</accession>
    <name>A0A3B5MEN7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEN7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022738"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023014.1">
        <property type="protein sequence ID" value="ENSXCOP00000022738.1"/>
        <property type="gene ID" value="ENSXCOG00000016986.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111138"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031937">
        <property type="entry name" value="PNISR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31518">
        <property type="entry name" value="ARGININE/SERINE-RICH PROTEIN PNISR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="121" mass="13520" checksum="FC7BAF41606D663A" modified="2018-12-05" version="1">MWDQGGQPWPQWPLSSQQQWMQSFQHQHDPGQVDWAALAQAWIAQKESTGTQPHNIQPNGQDLPGHDSGPPAPPANHGAFQGEPGFGRMWQPGKRWKGWGGYFDLTVGFPESHFSGQFCSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEP4</accession>
    <name>A0A3B5MEP4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Elastase 3 like</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEP4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022142"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022411.1">
        <property type="protein sequence ID" value="ENSXCOP00000022142.1"/>
        <property type="gene ID" value="ENSXCOG00000016510.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234528"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000004">
        <property type="entry name" value="Tryptase gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050850">
        <property type="entry name" value="Peptidase_S1_Elastase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24257">
        <property type="entry name" value="CHYMOTRYPSIN-LIKE ELASTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24257:SF31">
        <property type="entry name" value="ELASTASE 3 LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="27"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="274" mass="29876" checksum="1CA1963BF94AE20D" modified="2018-12-05" version="1">LAAHLKLNQIRSLGCGTPPVEPLTSRVVNGVDAKPHSWPWQISLQYERDGEWRHSCGGSLIAANWVMTAAHCINTKFSYRVFVGKHNLVEEEAGSKAILPEKIIVHEKWHPIFVAFGNDIALIKLSEPVTLSDQVQLGCIPAPGTLLTNLYPCYITGWGRVYTGGPIADKLQQALMPVADHATCSQSDWWGIAVRTSMVCAGGDGIVAGCNGDSGGPLNCKNAEGFWEIHGIASFVSGFGCNYEKKPTVFTRVSAFNSWIDQVKKMADVFLKLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFX8</accession>
    <name>A0A3B5MFX8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PER33/POM33 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MFX8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020021"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020268.1">
        <property type="protein sequence ID" value="ENSXCOP00000020021.1"/>
        <property type="gene ID" value="ENSXCOG00000015027.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011368"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071786">
        <property type="term" value="P:endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051645">
        <property type="entry name" value="PER33/POM33_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005344">
        <property type="entry name" value="TMEM33/Pom33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12703:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03661">
        <property type="entry name" value="TMEM33_Pom33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="223" mass="25934" checksum="C160C61043E23C97" modified="2018-12-05" version="1">MSNKLETAMWLSRLFTVYFHHVTLYHTANFYQRALLANALTSALRLHQRLPRFQLSRAFLAQALQEDSCHYLLYSLILVNSYPITMSIFPVFLFSLLHATTYTKKVLDSMGPGSLMFIRNLLDRLTANQQNILKFIACNEIFLMPATVFMLFSGQGSLLLPFIYYRFLTLRYTSRRNPYCRTLFTELRILLEHFIMKPSCPAFLRRMCLSSIAFVSRLAPTGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHS5</accession>
    <name>A0A3B5MHS5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NALCN channel auxiliary factor 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NALF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027867317.1">
        <property type="nucleotide sequence ID" value="XM_028011516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHS5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023302"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023581.1">
        <property type="protein sequence ID" value="ENSXCOP00000023302.1"/>
        <property type="gene ID" value="ENSXCOG00000017404.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114141090"/>
    <dbReference type="KEGG" id="xco:114141090"/>
    <dbReference type="GeneTree" id="ENSGT00940000155696"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015275">
        <property type="term" value="F:stretch-activated, monoatomic cation-selective, calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055288">
        <property type="entry name" value="NALCN_aux_factor_1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15819:SF9">
        <property type="entry name" value="NALCN CHANNEL AUXILIARY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15819">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN FAM155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="385" mass="43012" checksum="2A1FD3BCE4B64C96" modified="2018-12-05" version="1">MTRGAGTCWQQEDDDGFQIWLEPPRDHQKPFTDSERAQRWRLSLASLLFLTVLLSDRLWFCAEARLARTPDKFASSHLRSPHTHSVHISLTENPHAIFLGNSTKHLCRLETRHRDSSSGDCFAFADADDLCRGLSDKQGPRVANMSDFYLSFCNSYSLLDLFDGSASPDNLNCSVRALKDGDATRCGECVQAFQRYDQHAQEKYEDFELLTEKYEPGAYSVRTCMEQCKMAYKPWLCAQYFPNIKQSCQRKIPCHRYCLEVQQSCPFILPDNDDLIHGGNPSFICTGLLGDHPSDGEAECCDVRWDSKMDSPSGSTLQRTASSCQPEGASVTSAASPRLCSGRLKICLLALVLLQTVIIISASHNSTLLDVAAIFSPEEAAPNEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJC2</accession>
    <name>A0A3B5MJC2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Protein kinase C and casein kinase substrate in neurons 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Ruffle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027859531.1">
        <property type="nucleotide sequence ID" value="XM_028003730.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027859532.1">
        <property type="nucleotide sequence ID" value="XM_028003731.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027859533.1">
        <property type="nucleotide sequence ID" value="XM_028003732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJC2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021221"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021481.1">
        <property type="protein sequence ID" value="ENSXCOP00000021221.1"/>
        <property type="gene ID" value="ENSXCOG00000015878.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114135994"/>
    <dbReference type="KEGG" id="xco:114135994"/>
    <dbReference type="CTD" id="619246"/>
    <dbReference type="GeneTree" id="ENSGT00950000182973"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060088">
        <property type="term" value="P:auditory receptor cell stereocilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048812">
        <property type="term" value="P:neuron projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900006">
        <property type="term" value="P:positive regulation of dendrite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030100">
        <property type="term" value="P:regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11998">
        <property type="entry name" value="SH3_PACSIN1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000014">
        <property type="entry name" value="Kinase C and casein kinase substrate in neurons protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000205">
        <property type="entry name" value="Protein kinase C and casein kinase substrate in neurons protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035743">
        <property type="entry name" value="PACSIN1/PACSIN2_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF16">
        <property type="entry name" value="PROTEIN KINASE C AND CASEIN KINASE SUBSTRATE IN NEURONS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="17">
        <location>
            <begin position="12"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="384"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="331"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="343"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="365"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="443" mass="51315" checksum="EDACED7D549A632D" modified="2018-12-05" version="1">MSGGYDEYASQEEATDSFWEVGNYKRTVKRIDDGNRLCNDLMNCIQERAKIEKGYAQQLTDWSKRWRQLVDKGPQYGSVEQAWMAVMTEAEKVSDLHTEMKNHLMSEDFEKVKNWQKDSYHKQMMGGFKESKEADEGFKKAQKPWAKKLKEVEVAKKSYHMACKEEKLATTREANTKGDGTTPAEQKKLQDKLEKCKQDSQKTREKYEKALDELNKCSPQYMENMEQVFNQCQQFEEKRLSFLREVLLDVKRHLNLTENQSYVTIYRDLERVITAASPEEDLKWFSSIHGPGMHMNWPKFEEYNPDLIHTISKREKSKKYNDEVMLTNVKAPRSNVQAEDRGSVSSYEKNQAYSSNEWSDDEGKGLNSGSDSNGGSNPFYEESGEGVRVKALYDYEGQEQDELSFKAGDLLTKLEDEDDQGWCKGRLDNGQLGLYPANYVEPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5ML99</accession>
    <name>A0A3B5ML99_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL22m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L22, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5ML99"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025174"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025479.1">
        <property type="protein sequence ID" value="ENSXCOP00000025174.1"/>
        <property type="gene ID" value="ENSXCOG00000018806.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002110"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.10:FF:000009">
        <property type="entry name" value="39S ribosomal protein L22, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047867">
        <property type="entry name" value="Ribosomal_uL22_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501">
        <property type="entry name" value="CHLOROPLAST 50S RIBOSOMAL PROTEIN L22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL22M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="211" mass="24655" checksum="F903639FEDFF89A5" modified="2018-12-05" version="1">MLFAIHKVNSASTKFFCSLFYRHGYRLQVLGGASQQLSCLHTSISLDDKHWEKNNRKVYPPQLPEEPRRPAEVYHSRRQIKYSKDKMWYLAKMIKGMSIDEAIAQLEFNDKKGAKVMREVLLEAQEMAVKNHNVEYKSNLYVAESFSGKGQYLKRIRYHGRGMFGIMDKVYCHYFVKLVEGVPPKPEERTGFDQAKEYVQGLKNRTIIHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPH8</accession>
    <name>A0A3B5MPH8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TNFR-Cys domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MPH8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025693"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026002.1">
        <property type="protein sequence ID" value="ENSXCOP00000025693.1"/>
        <property type="gene ID" value="ENSXCOG00000019198.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155167"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052459">
        <property type="entry name" value="TNFRSF_decoy_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23097">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23097:SF116">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00020">
        <property type="entry name" value="TNFR_c6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00208">
        <property type="entry name" value="TNFR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57586">
        <property type="entry name" value="TNF receptor-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50050">
        <property type="entry name" value="TNFR_NGFR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="TNFR-Cys" evidence="9">
        <location>
            <begin position="43"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="8">
        <location>
            <begin position="43"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="9">
        <location>
            <begin position="84"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="8">
        <location>
            <begin position="84"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="182" mass="20136" checksum="DBDA1B62479FB09F" modified="2018-12-05" version="1">MEFILTTIITDPITGNPLQCDRCPPGTFLRASCSSIKKSECVPCPQDSFTELWNYMGRCLRCGVCGRNQVVKKECTANSDCQCECKEGYFYHQDYDMCVRHSECPSGQGVLTKGTAERDTVCSVCSEDTFSDISSTHHNCTQHKSCSDAGLQLVLRGSSWHDSVCANCQQNYSTSSTALKHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQ55</accession>
    <name>A0A3B5MQ55_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Abhydrolase domain containing 16A, phospholipase</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. ABHD16 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQ55"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025661"/>
    <dbReference type="ESTHER" id="xipma-a0a3b5r3p5">
        <property type="family name" value="ABHD16"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSXCOT00000025970.1">
        <property type="protein sequence ID" value="ENSXCOP00000025661.1"/>
        <property type="gene ID" value="ENSXCOG00000019167.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160908"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052651">
        <property type="term" value="P:monoacylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006660">
        <property type="term" value="P:phosphatidylserine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000074">
        <property type="entry name" value="Abhydrolase domain containing 16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054518">
        <property type="entry name" value="ABHD16_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF72">
        <property type="entry name" value="BAT5L PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22990">
        <property type="entry name" value="ABHD16_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidylserine Lipase ABHD16 N-terminal" evidence="5">
        <location>
            <begin position="62"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="274"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="558" mass="63004" checksum="27EE5E133B0500E3" modified="2018-12-05" version="1">MLALAHLASSSALSGMELPTQESGKTHRQHSWLGQYNILLHLLGESIKLLNESVIILYTLQASAIWSISYYSSPLLLCYLYRKGYICSSKLVPVSQYLGTVLVCLLGVACIRGWGRWRNTDYVQFISVLEETKKNHTPANKEKLRCFNFDFSHWPSDFSWTEVSSPKHPKGGVSLLKPEPRLRGTADGFLHSVRTLPCHVIGFLIVHSFGRRMLYPGSVGLLQKAMRPMLQGGQAKLIEEYDGERNKLVACDGNEIDTMFVDRRKDGGPRGRTLVICCEGNAGFYEVGCMNTPLEGGYSVLGWNHPGFAGSTGVPFPQNEANAMDVVIQFAVHKLGFQLSDIVIYAWSIGGFTASWAVMSYPEIQSIVLDASFDDLLPLALKVMPESWRPLVQHTVRQYMNLNNAEQLLKYQGPVLLVRRTRDEIITTTHPEDIMSNRGNDLLLRLLQFRYPKIMTEEGVRVVRQWLGASSPIEEASVYSGYEVDDDWCLSVLKSYQTDRDVSFPWSVGEPAHRDDELQTELRTDEMLSLSARKYMRNFESTHCTPLPAAEFHPPWRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQE4</accession>
    <name>A0A3B5MQE4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQE4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025741"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026051.1">
        <property type="protein sequence ID" value="ENSXCOP00000025741.1"/>
        <property type="gene ID" value="ENSXCOG00000019239.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154621"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010820">
        <property type="term" value="P:positive regulation of T cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000114">
        <property type="entry name" value="serine/threonine-protein kinase OSR1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF14">
        <property type="entry name" value="STE20_SPS1-RELATED PROLINE-ALANINE-RICH PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="27"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="113" mass="12708" checksum="C3AE31E20B5F1D7E" modified="2018-12-05" version="1">MADQGESPVPVHHPQPTISWPITRESYELQEVIGSGATAVVQAALCTPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCNHPNIVTYYTSFVVKDELWLVMKLLSGGEFSMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MS47</accession>
    <name>A0A3B5MS47_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Focal adhesion kinase 1</fullName>
            <ecNumber evidence="4">2.7.10.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Protein-tyrosine kinase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">pp125FAK</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MS47"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026618"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026942.1">
        <property type="protein sequence ID" value="ENSXCOP00000026618.1"/>
        <property type="gene ID" value="ENSXCOG00000019872.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155113"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007172">
        <property type="term" value="P:signal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13190">
        <property type="entry name" value="FERM_C_FAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05056">
        <property type="entry name" value="PTKc_FAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.330:FF:000001">
        <property type="entry name" value="focal adhesion kinase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000058">
        <property type="entry name" value="focal adhesion kinase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000021">
        <property type="entry name" value="focal adhesion kinase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000047">
        <property type="entry name" value="focal adhesion kinase 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000039">
        <property type="entry name" value="Focal adhesion kinase, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000004">
        <property type="entry name" value="Protein-tyrosine kinase 2-beta isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.540">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041390">
        <property type="entry name" value="FADK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049385">
        <property type="entry name" value="FAK1-like_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041784">
        <property type="entry name" value="FAK1/PYK2_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036137">
        <property type="entry name" value="Focal_adhe_kin_target_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005189">
        <property type="entry name" value="Focal_adhesion_kin_target_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46221">
        <property type="entry name" value="FERM AND PDZ DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46221:SF9">
        <property type="entry name" value="NON-SPECIFIC PROTEIN-TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21477">
        <property type="entry name" value="FERM_C_FAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18038">
        <property type="entry name" value="FERM_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03623">
        <property type="entry name" value="Focal_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68993">
        <property type="entry name" value="FAT domain of focal adhesion kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="FERM" evidence="25">
        <location>
            <begin position="37"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="410"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="685"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="753"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="23">
        <location>
            <begin position="758"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00039644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00043012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="944" mass="107385" checksum="3E9AA76A596B3510" modified="2018-12-05" version="1">MATAYMDPNLNNAFLAGTKSRLSAGGSDRTMAGSVDRVLKVFHYFEANTEHSFWSSNIRYGDATDVRGIIQKILDINKVRWTSCFGLRLSNSQSRDQLHWLHPDMGVSHIREKYEQARPNEEWRYELRIRYLPKGFVQQFTEDKPTLNYFYHQVKNDYMTQSGDQAEQDVALKLGCLEIRRFFKEMRGNALDKKSNYELLEKDVGLRRFFPKDLLDSVKAKTLRKLIQQTFKQVANLNDEQCILKFLEILAPIYRYDKEFFKCALGSSWVIQVELAIGPEEGISYLTDKGSTPTHLANFNQVQSIQYSAMEEKDRKGMLQLNVAGAAEPLTVTTASLTTAENLADLIDGYCRLVSMETQSFIVRVQKGGLFLYSSISNLAETDDYAEIVDEEDTYTMPSTRDYEIQRDRIDLGRCIGEGQFGDVHQGVYNCPDKPPLPVAIKTCKNCTTDSVREKFLQEALTMRQFDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQVKKYSLDLSTLILFSYQLSTALAYLESKRFVHRDIAARNVLVSSVDCVMLGDFGLSRYMEDSSYYKASKGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMYGIKPFQGVKNNDVIGRIENGERLAMPPQCPPTLYSLMTKCWSYDPSKRPRFTELKTQLSAILEEEKSIQEERLREEMRRQVTVSWDSGGSDEAPPKPSRPGYPSPRISGSFFSTSQHIPFQCFSLQKPESRNSRGSIDREDGTLQAPVSQKHFLHEKHVAPPKKPPRPGAPLTNLANLSPVDSYNDGVKLQPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSNRIQPAAPEEYVPMVKDVGLALRTLLATVDETIPVLPASTHREIEMAQKLLNSDLAELISKMKLAQQYVMTSLQKDYKKQMLMAAHALAVDAKNLLDVIDQSRLKMISQIRPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSZ8</accession>
    <name>A0A3B5MSZ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Suppression of tumorigenicity 14b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSZ8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022624"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022900.1">
        <property type="protein sequence ID" value="ENSXCOP00000022624.1"/>
        <property type="gene ID" value="ENSXCOG00000016911.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164481"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.960">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF51">
        <property type="entry name" value="CUBILIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50024">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="SEA" evidence="6">
        <location>
            <begin position="57"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="163"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="302"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="389"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="401"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="426"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="438"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="456"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="463"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="475"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="570" mass="62670" checksum="8C593F1F008E2984" modified="2018-12-05" version="1">DVSEQFLQAPDSRDMEKDPGRRRRLWAGLGLLVAAGALALLTGLLVWHFHLRRDVRMTKLYVGSVGIGGRSFRADLEEPGSDGFSSLAQKVRAQLQQIYGQNSVLAKFYKDSTVQAFRSPDGMVAYYESEFELPVVQLSSLDQAMASLEEMGPVLEEIRLDPCRTVHPNLADFSEKAPISIHVRKNGHIDSPGFPDAPYGPNTAQKWQLRADPGHRVRLDFHTLILEDDCEQDFVQIYDSLAPIRPRLLTEQCGYPHGSLAFVSSGNVMLLMLVTSQDKNFPGFRVFYSQVPAAGGASVVYCGGTLSGERGSFSSPFFPANYPPKADCSWTVQVPEGRFVKVQFQKFSVGKDGSPCLHDYVTIDEERTRLDLLFQRPVRGFPCPGKFRCGTNLCINTTLRCDGFNDCGDNHDEIGCSDHCWLSMKCKNNICKAKFWMCDGVDDCGDNSDEDGCGKCGAAEFSCRNGHCVSDALRCDGRDDCGDASDESTCQKCKDTRTGSEPVQNQARTGPALSWFRPQWGGAHQNRVCCTSASTGMRAEPSGLTCRSRTAALWAAGELKGGTSTCRGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUF9</accession>
    <name>A0A3B5MUF9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Plays a role in regulating ADIPOQ and FABP4 levels in differentiating adipocytes and is also involved in regulation of adipocyte triglyceride storage. Weakly binds 25-hydroxycholesterol. Interacts with OSBPL9 to function as lipid transfer proteins. Together they form a heterodimer that localizes at the ER-trans-Golgi membrane contact sites, and exchanges phosphatidylserine (1,2-diacyl-sn-glycero-3-phospho-L-serine, PS) for phosphatidylinositol-4-phosphate (1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate), PI(4)P) between the two organelles, a step that is critical for sphingomyelin synthesis in the Golgi complex.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(out) + a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)(in) + a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:81667"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterodimer with OSBPL9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Late endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MUF9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027398"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027731.1">
        <property type="protein sequence ID" value="ENSXCOP00000027398.1"/>
        <property type="gene ID" value="ENSXCOG00000020455.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158398"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13291">
        <property type="entry name" value="PH_ORP10_ORP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2720:FF:000001">
        <property type="entry name" value="Oxysterol-binding OBPalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000154">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000002">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3490:FF:000001">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF46">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 15" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="17">
        <location>
            <begin position="43"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="668"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004198"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004414"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00050284"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00058048"/>
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            <dbReference type="ARBA" id="ARBA00065555"/>
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            <dbReference type="RuleBase" id="RU003844"/>
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            <dbReference type="RuleBase" id="RU003845"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50003"/>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027398.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="719" mass="80614" checksum="C4962162AD93ECC5" modified="2018-12-05" version="1">TMQGETAAVRVAENEGKVDAFPPNRTPSSGRAGGKSWQYSDHMENIDGYLMKYTNLVTGWQYRFFVLNNEAGLLEYFVNEQSRSQKPRGSLPLAGAVISPSDEDSHTFTVNAISGEQYKLRASDAKERQHWVSRLQICTQHHTEALGKVGKVPPGSRSDPDRASSPMSLRRPSQSASALFGWTQNHKGSVYSSRKSLMPDHLLDAREMMSQAQGQHRDLIQSIEGLPAAPGPGLSPLNQDLLMLKATSMATMSCLSECLHILQLQQVAQQRAALGGPTIEWLEPKVADDVLKNGSSPLGNHTPLDGGRLPLSAPDALSFALGFVWQEQEVVDPEDEVEDSFMDTEEDLGAVEEERSVILHLLSQLKLGMDLTRVVLPTFILEKRSLLEMYADFMSHPELFVTVTDGNSPEDRMIRFVEYYLTSFHEGRKGAIAKKPYNPIIGETFHCSWRVPRGPEALQGEAEPPAAAPEPYQVRFVAEQVSHHPPVSGFYAECPERQMCVNTHVWTKSKFMGMSIGVSMIGEGCLHLLEHDEEYTFTLPCAYARSILTVPWVELGGKVNIACNKSGYSAVITFQTKPFYGGKLHKVTAEVKHNSTNAVVCRVQGEWNGVLEFSYTSGETKVVDVTKLPVTRKRVRPIEKQAPTESRRLWQHVTEALRQKDIDKATEHKRVLEERQRAEERQRAETETPWRTRYFDREGEGWAYSKPLWKNSKPYVSCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MV07</accession>
    <name>A0A3B5MV07_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">2-oxoglutarate dehydrogenase complex component E1</fullName>
            <ecNumber evidence="6">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Alpha-ketoglutarate dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Thiamine diphosphate (ThDP)-dependent 2-oxoglutarate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 2-oxoglutarate + H(+) = N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:12188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:20092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="1.2.4.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:12189"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MV07"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023349"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023629.1">
        <property type="protein sequence ID" value="ENSXCOP00000023349.1"/>
        <property type="gene ID" value="ENSXCOG00000017434.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183125"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02016">
        <property type="entry name" value="TPP_E1_OGDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000007">
        <property type="entry name" value="2-oxoglutarate dehydrogenase e1 mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1150:FF:000001">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="entry name" value="Multifunctional 2-oxoglutarate metabolism enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1150">
        <property type="entry name" value="TPP helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008907">
        <property type="entry name" value="PRK12270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF7">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="16" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="23">
        <location>
            <begin position="646"/>
            <end position="861"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00030680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00040429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00041946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00042799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1019" mass="114633" checksum="B093335ADE10F51B" modified="2018-12-05" version="1">MHRLRTTVARLGPLAAAQTAQTAPQSRLLALEGGARTFQPVRSLSASVAAEPFLNGTSSNYVEEMYYAWLEDRRNVHKSWDIFFRNANAGAPPGAAYQSPPPLSGPSDGMTGVRMLVGAQPNVEKLVKDHLAVQSLIRAYQVRGHHIAKLDPLGISCVDFDDAPCTVGLYGLTEGDLDKVFRLPASTFIGGNASALPLREIIRRLEMAYCQHIGVEFMFINDVEQCQWIRQKFETPGIMQFSLEEKRTLLGRMIRSTRFEEFLQRKWSSEKRFGLEGCESLIPALKTIIDRSSQCGVESVILGMPHRGKLNVLANVIRKDLDQIFCQFDSKLEAADEGSGDVKYHLGMYHRRMNRVSDRYITLSLMANPSHLEAVDPVVQGKTKAEQFYSGDTEGKRVMSILLHGDAAFAGQGIVYETFHLSDLPSYTTHGTIHVVVNNQIGFTTDPRMARSSPYPTDVARVVNAPIFHVNADDPEAVMFVCNVAAEWRNTFHKDVVVDLVSYRRNGHNEMDEPMFTQPLMYKQIKKQKGVLQTFVEKLVAEGVVTTQHYEEEVARYDKICEDAFAHSKDEKILHIKHWLDSPWPGFFTLDGQPKTMSCPSTGIREDELCHIGNIAASVPVKDFMIHGGLSRILKGRADMVSQRVCDWALGEYMAFGSLLKEGIHVRLSGQDVERGTFSHRHHVLHDQNVDKRICIPMNSISPDQAPYTVCNSSLSEYGVLGFELGFAMASPKALVLWEAQFGDFHNTAQCIIDQFISSGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVFPVSLFTGDFAVHQLYDCNWIVANCSTPANYFHVLRRQILLPFRKPLIVFTPKSLLRHPEAKSSFDNMLPGTHFQRLIPDEGPAAVGPQNVKRVVFCTGKIYYDLIRERKSRGLEDAVAVVRIEQLSPFPFDLVKAEADRFQNADLVWCQEEHKNQGYYLYVKPRLRTTINRKRPIWYAGREPAAAPATGNKHTHLTELQRLLDAAFDLETFPGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MV88</accession>
    <name>A0A3B5MV88_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Secretin receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MV88"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023439"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023720.1">
        <property type="protein sequence ID" value="ENSXCOP00000023439.1"/>
        <property type="gene ID" value="ENSXCOG00000017500.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156402"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015055">
        <property type="term" value="F:secretin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF13">
        <property type="entry name" value="SECRETIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111418">
        <property type="entry name" value="Hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="219"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="13">
        <location>
            <begin position="8"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="14">
        <location>
            <begin position="106"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="382"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="389"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="402"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="423" mass="49420" checksum="C6231E763057DD0F" modified="2018-12-05" version="1">DPMKEEEKCMNDLILFMEQKAKVNSSVYCKGMWEDMHCWPPASLGETVSLPCLDIFGRVSKVHRNCTSNGWTDLLSLHEDACSYAFNDSLKYLEHFSASHWYFSYVKTMYTIGYTLSLITLAIAITIFCFFRKLHCTRNYIHIQLFVSFILRAIFIFIRDGLLFTDSELYDCDSSTVACKIVLMFCNYFILANYSWLLVEGHFLFTLVSRSFFSLKKHLAWSIFLSWGIPFIIIVCWGCAKYFYEDEGCWERRRHEWIWWILRVPVLLSICMNLVFFLGILRILISKLRMSDAHRSEFSQYMKLIKSTFFLVALFGLHCILFVFLPVNVSNSVFKIWTFAELALSSTQGFVVAVLYCFMNGEVQQEFKRRWKRWKLTRHLPSRSRQHHGSISQIGSPHTQVSLLPSSPTSLTNTGFPADTLEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVQ4</accession>
    <name>A0A3B5MVQ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Shisa N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVQ4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025407"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025714.1">
        <property type="protein sequence ID" value="ENSXCOP00000025407.1"/>
        <property type="gene ID" value="ENSXCOG00000018977.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162254"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032281">
        <property type="term" value="C:AMPA glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032591">
        <property type="term" value="C:dendritic spine membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048172">
        <property type="term" value="P:regulation of short-term neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026910">
        <property type="entry name" value="Shisa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053891">
        <property type="entry name" value="Shisa_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31774:SF2">
        <property type="entry name" value="PROTEIN SHISA-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31774">
        <property type="entry name" value="PROTEIN SHISA-9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13908">
        <property type="entry name" value="Shisa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017372663" description="Shisa N-terminal domain-containing protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Shisa N-terminal" evidence="6">
        <location>
            <begin position="19"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="91" mass="10759" checksum="AACBCB244AA2968F" modified="2018-12-05" version="1">FCYLLSCLVFLFYALFPRGYYDVMGHFDNTFNCSKGTYRYCCGTCQYRFCCEHRRNRLDQESCKNYISPVWADPQAPVSVRLSKKNPTDVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXY0</accession>
    <name>A0A3B5MXY0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="Apolipoprotein A-I"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
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            <dbReference type="Ensembl" id="ENSXCOP00000026647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="104" mass="12239" checksum="02A3CF6B3BBFB9D0" modified="2018-12-05" version="1">FAENRVNRMSQYIENIKTKISHDFHDKTANLMFRSFLRSFVEDRQTELEPLAVKIQGQLQDMEEHFKPLTENVKENVNPFIASLQQKMESILQMLEEHANASSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MY15</accession>
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            <fullName evidence="7">Cytosolic arginine sensor for mTORC1 subunit 2</fullName>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <citation type="submission" date="2025-09" db="UniProtKB">
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        <text evidence="2">Belongs to the GATS family.</text>
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        <property type="entry name" value="CASTOR_mTORC1"/>
    </dbReference>
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        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="9"/>
            <end position="68"/>
        </location>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="291" mass="31861" checksum="0980ACC251B54F7F" modified="2018-12-05" version="1">MELHILEHSLKVASIEKEGIEICTHGLIKLAFLASKTRCKFFSLTETPEDYTIIVDEEGFKELPQSNLISVADSTWLALNVVSGGGNASSSQPIGVTKIAKSVIAPLADHNISVFMLSTYQTDFILVRERDLPMVMHTLSSEFTLFRVVNGETVAAHNLGISNGFVKPKLVPRPIIHPLSSPSNMFCVTSLDPDTLPSVATLLMDVMFYSRGFPNNVLFTSASGELWKMVRIGGQPLGFDECGIVAQISEPLATADIPAYYISTFKFDHALVPEENIQSVIRALRTESTAQ</sequence>
</entry>
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    <accession>A0A3B5MYH8</accession>
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            <fullName evidence="3">F-box domain-containing protein</fullName>
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    </dbReference>
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            <begin position="1"/>
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    </evidence>
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        <source>
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    <sequence length="439" mass="50066" checksum="32347CBE3FC32471" modified="2018-12-05" version="1">MERNREIQTLSNTLLDSAEPLKGHGERKKKRNPDLLSRDPKAVKEEEDNRFPEEDRWGQLLPEEVLVNIFQMLVVQDGAVPFLCRVSRVCRLWNAAAATLTLKPPDALKYESLFFFRFSQLRDFTLCHWATSVDFALETVSEFCPHLRSVHLSYCRGVTGSGLRSLGLHSRTLVFPLLQFQVEGLVDFLEEHGKFMFLCLPTALNSSYRSMRLSLQKGFCPDLELLEVNKKLDSKDTELSVCFQSLQKACPKLKAMRKRDESEAGFPLLDEFCIANTEYSYITDQVLRDVLFGSTRLRVLDLRGSTRITTFGLAQLPCSKLECLFWGQYFTKNIGFTSVKKNLQLLMEKWGPTLQQLDITNQPFTEADLEVALTFLTQVAEADTLLSLNLSGTKITAPVIEQMTALGYLNLSSCRNLPRGVKKIYRSQEEIRELLANLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N1T9</accession>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017183779" description="Chemokine interleukin-8-like domain-containing protein" evidence="2">
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            <begin position="22"/>
            <end position="93"/>
        </location>
    </feature>
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        <location>
            <begin position="26"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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            <dbReference type="Pfam" id="PF00048"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="93" mass="10308" checksum="582EB978988E0BA4" modified="2018-12-05" version="1">MGYHNVLHCILCCVLTQGPNALKPGKCCFHFFTGRIPVGEIRSIAKTHENCSKRGFVINTPRGDYCVDMNQKWAIKAFLKTPKQVATSASLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N218</accession>
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        <recommendedName>
            <fullName evidence="9">C-type lectin domain-containing protein</fullName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N218"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027980"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028320.1">
        <property type="protein sequence ID" value="ENSXCOP00000027980.1"/>
        <property type="gene ID" value="ENSXCOG00000020876.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154368"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051663">
        <property type="entry name" value="CLec_Tetranectin-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22799:SF1">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 11 MEMBER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22799">
        <property type="entry name" value="TETRANECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0176">Collagen</keyword>
    <keyword evidence="4" id="KW-0430">Lectin</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017347212" description="C-type lectin domain-containing protein" evidence="8">
        <location>
            <begin position="24"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="9">
        <location>
            <begin position="119"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="45"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="232" mass="25281" checksum="ABD477519128994E" modified="2018-12-05" version="1">FSLFTSLISLEMMLLTYKVLIHCRVFCSVFFLPFYSHSGLPGPDGKPGLPGPDGKPGLPGLDGKPGLPGPDGKPGLPGLDGKPGLAGLCGPSVNIAALMAGEAEQYFPLLFVRKVGEKYFVSYKEREPFEKAVNFCSQRGLELALPQSEEENNALAEVFDDAYQTVWINVNKKKAQGDFTLDANDRPLTFAKWGDGQPDASVQDVGCTMVSRNGFWKVTKECFLNAYVVCQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N229</accession>
    <name>A0A3B5N229_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Pseudouridylate synthase 7 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Pseudouridylate synthase that catalyzes pseudouridylation of RNAs. Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs): pseudouridylated tRFs inhibit translation by targeting the translation initiation complex. Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3'. Acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions. Pseudouridylation of pre-mRNAs near splice sites directly regulates mRNA splicing and mRNA 3'-end processing. In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a uridine in tRNA = a pseudouridine in tRNA</text>
            <dbReference type="Rhea" id="RHEA:54572"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13934"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>uridine(13) in tRNA = pseudouridine(13) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42540"/>
            <dbReference type="Rhea" id="RHEA-COMP:10105"/>
            <dbReference type="Rhea" id="RHEA-COMP:10106"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with SIRT1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N229"/>
    <dbReference type="SMR" id="A0A3B5N229"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000028015"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028356.1">
        <property type="protein sequence ID" value="ENSXCOP00000028015.1"/>
        <property type="gene ID" value="ENSXCOG00000020897.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063554"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02576">
        <property type="entry name" value="PseudoU_synth_ScPUS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.20:FF:000002">
        <property type="entry name" value="Pseudouridylate synthase 7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.20:FF:000003">
        <property type="entry name" value="Pseudouridylate synthase 7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="entry name" value="TruD, catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020119">
        <property type="entry name" value="PsdUridine_synth_TruD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00094">
        <property type="entry name" value="tRNA_TruD_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326:SF31">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE 7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037016">
        <property type="entry name" value="Pseudouridin_synth_euk_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01268">
        <property type="entry name" value="UPF0024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TRUD" evidence="16">
        <location>
            <begin position="338"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00063455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00070906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000028015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="627" mass="70804" checksum="8B6A22672F0ABF66" modified="2018-12-05" version="1">MAESEPVSDAAPVGEKRTCLRHGDEETPAKKPKVDGENGNAPNPDGGGGGGEEEEEEQPEGQASDGEEDGESFADMMKHGLTETDVGILKYVSDHKGFSGILKERYSDFVVHEINKQGKMVHLDDLSIPADSEQQPTECDVLTEEQRQQLGELQLFKNKERNVSIEVKDDTKEKRTLVHKAIKTQFPGLETKTEEKEGHKFIVAYHAAGKKALAAPRKHFWPKNRGSYCHFALYKENKDTMDAINVLSKLRPNMFSYMGTKDKRAITVQEIAVLKITAERLAHLNKCLMNLKLGNFCYKNHPLKLGELQGNHFTVVIRNISGTDEQVQQAMGSLKQTGFINYYGMQRFGTTAVPTQQVGRAILRNDWNQVVDLILKPRPGAEKEYLVRCREEWAKTQDPEAALKKLPNKRCVEGQLLRGLSMYGKKNIVTAFGLIPRNNRLMYVHSYQSVVWNAMASRRIEAFGLKAVEGDLVLRGTHVLSAEEAESHSIHDIVMPLPGYDVIYPSHHIGEGYRELLSADGLDLDNMRHKVKDYSLAGAYRRIIVRPTDVSWLVIHYDDPRISLVHSDFEKLENKPAPVFNQDGQYRALRMEFSLPSSTYATMAIREVLKLDTSIKKQTQLNTVWFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KK56</accession>
    <name>A0A3B5KK56_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Xenotropic and polytropic retrovirus receptor 1a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYG1 (TC 2.A.94) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KK56"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000558"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000567.1">
        <property type="protein sequence ID" value="ENSXCOP00000000558.1"/>
        <property type="gene ID" value="ENSXCOG00000000479.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164519"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14477">
        <property type="entry name" value="SPX_XPR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF132">
        <property type="entry name" value="SI:DKEY-6N6.7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="440"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="10">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="9">
        <location>
            <begin position="367"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="594"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="594"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="625" mass="73544" checksum="97AC595A320DCE6A" modified="2018-12-05" version="1">MKFAEHLSSHITPEWRKQYLRYEALKEMLYSAQDHAPSIEVTDEDTVKRYYAKFEERFFQNCEKELLKINTFYSEKLAEAQRRFATLQNELQSSMDAQREQNTPYGLRKRKTLFHLTQEERSKHHNIMDLKLAFSEFYLSLILLQNYQNLNFTGFRKIVKKHDKILDTTRGVEWRLAHVDVAPFYTCKKITQLITETETLVITELEEGDRQRAMKRLRVPPLGAAQPALAWTTFRVGLFCGFFIILVVAFILTGAVVTRYENIWPLVRIYRGGFLLIQFLFLLGINTYGWRQNGVNHVLIFEIDPRDNLSHQHLFEIAGFLGVLWCLSILSCLYSEYTYLPMQVNPLILYGFMVLFLINPFKTCYYKSRFWLIKLLFRVFTAPFHRVEFADFWLADQLNSLVVVLMDFEYLICFYIFELQWSSSKGLIPPEQGHTDADMFLYLLIVFSTISSLYTLTWDLIMDWGLFDRGAGENTFLREEIVYPHKAYYYFAIVEDVLLRFAWTVQISLSTMTDPHSVGNIVATILAPLEVFRRFVWNFFRLENEHLNNCGEFRAVRDISVAPLNADDQTVLEQMMDQEDGIRNRSGKTWKRNQSLSLRRPQLSSSHSKKDTKVLIEDTDDEAFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KLQ0</accession>
    <name>A0A3B5KLQ0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Tumor necrosis factor receptor superfamily, member 21</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KLQ0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001052.1">
        <property type="protein sequence ID" value="ENSXCOP00000001038.1"/>
        <property type="gene ID" value="ENSXCOG00000000843.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156212"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006959">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030889">
        <property type="term" value="P:negative regulation of B cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031642">
        <property type="term" value="P:negative regulation of myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051402">
        <property type="term" value="P:neuron apoptotic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:oligodendrocyte apoptotic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="tumor necrosis factor receptor superfamily member 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000011">
        <property type="entry name" value="Tumor necrosis factor receptor superfamily member 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001368">
        <property type="entry name" value="TNFR/NGFR_Cys_rich_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022330">
        <property type="entry name" value="TNFR_21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034034">
        <property type="entry name" value="TNFRSF21_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46921">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46921:SF1">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
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    </dbReference>
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        <property type="entry name" value="TNFR_c6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01971">
        <property type="entry name" value="TNFACTORR21"/>
    </dbReference>
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        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00208">
        <property type="entry name" value="TNFR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57586">
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00652">
        <property type="entry name" value="TNFR_NGFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50050">
        <property type="entry name" value="TNFR_NGFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017256428" evidence="9">
        <location>
            <begin position="27"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="TNFR-Cys" evidence="11">
        <location>
            <begin position="85"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="repeat" description="TNFR-Cys" evidence="6">
        <location>
            <begin position="85"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Death" evidence="10">
        <location>
            <begin position="372"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="202"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="86"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="609" mass="66781" checksum="0915DD2CCFF84F20" modified="2018-12-05" version="1">MEIIHILKQLTCCFLFSSQLLCTVVADISARALTTSTADDQPLLSPRHYQHTDRDSGTQLVCDKCPAGTYVSVHCSPTTVRECSPCPRGTFTRGENGVQQCHRCRAPCLAGFIEKVPCTATQDRVCTCPPNTFLSKGGGLDCKPYSLCPPGSRVKRRGTEKEDVLCKPCTKGTFSQVESSAMSCRNHTDCKALARLLLTPGTRQTDNVCGPPSATLSSVPPTTPLGPLQADLKKDLGESDADPEPGANKPRMEGAVGPEVVRVGTGISNVVNLGNAGEASNYYRPNRKGSTRPTMHDHFDINEHLPWMIVLLLLLVLVVIVVCSVKRSSRVLKKGPVQDPSSIMEKAIHKKTSGTPTQAREKWIYYYSNGQGVDILKLVAAQVGNQWVDIYQSLANATEREVAAFSNGYSSDHERAYAALQHWTIRDSGANLAKLINALHRLRRIDVVEKIRCVMEDNPQLDINQLTTTVNVSQSLSPIHKLLESPTGGVGPIQSPMDRTKGFFADESEPLLRCDSTSSKDSALSRNGSFITKEKKDTVLRQVRLDPCDLQPVFDDMLHILNPEELHVLEEIPTAEDKLDRLFEIAGVKSQEASQTLLDSVYSHLPDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQ85</accession>
    <name>A0A3B5KQ85_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Flavin-containing monooxygenase</fullName>
            <ecNumber evidence="18">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Broad spectrum monooxygenase that catalyzes the oxygenation of a wide variety of nitrogen- and sulfur-containing compounds including xenobiotics. Catalyzes the S-oxygenation of hypotaurine to produce taurine, an organic osmolyte involved in cell volume regulation as well as a variety of cytoprotective and developmental processes. In vitro, catalyzes the N-oxygenation of trimethylamine (TMA) to produce trimethylamine N-oxide (TMAO) and could therefore participate to the detoxification of this compound that is generated by the action of gut microbiota from dietary precursors such as choline, choline containing compounds, betaine or L-carnitine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N,N-dimethylaniline + NADPH + O2 + H(+) = N,N-dimethylaniline N-oxide + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:24468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16269"/>
            <dbReference type="ChEBI" id="CHEBI:17735"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:24469"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>hypotaurine + NADH + O2 + H(+) = taurine + NAD(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:74111"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57853"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
            <dbReference type="EC" id="1.14.13.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:74112"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>hypotaurine + NADPH + O2 + H(+) = taurine + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:69819"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57853"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:507393"/>
            <dbReference type="EC" id="1.14.13.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:69820"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>trimethylamine + NADPH + O2 = trimethylamine N-oxide + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:31979"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15724"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58389"/>
            <dbReference type="EC" id="1.14.13.148"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:31980"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQ85"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000632"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000642.1">
        <property type="protein sequence ID" value="ENSXCOP00000000632.1"/>
        <property type="gene ID" value="ENSXCOG00000000550.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160836"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047822">
        <property type="term" value="F:hypotaurine monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034899">
        <property type="term" value="F:trimethylamine monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000159">
        <property type="entry name" value="Dimethylaniline monooxygenase [N-oxide-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000960">
        <property type="entry name" value="Flavin_mOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002253">
        <property type="entry name" value="Flavin_mOase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000332">
        <property type="entry name" value="FMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00370">
        <property type="entry name" value="FMOXYGENASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01121">
        <property type="entry name" value="FMOXYGENASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 18" id="KW-0274">FAD</keyword>
    <keyword evidence="4 18" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 18" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="10 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="420" mass="47812" checksum="C6E2F5A2461D416F" modified="2018-12-05" version="1">MVRRVAVVGAGSSGLACVKVCLEEGLDPVCFESSDDIGGLWKFKESPEPDRSSIYRSLVVNTSKEMMCFSDFPMPDDYPNYMHNSQLLQYFRLYAEHFHLLQHIRFQTTVRSVTQRPDFSESGQWDVVTMNKNNEEERHIFDAVLVCSGHYTHPVLPLSDFLGHETFLGRLLHSREYRDADSFRGERVVVVGFGNSGGDVAVEISRSAEKTFLSVRDGFWVMSRMAHRGLPVDMALISRFNILLLQLLPKTLINWAAERALNQKYDHELYGLKPRHRLLDKRPLVNDDLPGRILQGALIMKPNIRAFKDSGVTFEDGTVEDNIDAVVFCTGYKGIFPFLPSALSEGPHSELTLYKRVFPPSLQQPTLAVMGFFQVRGPIMPIVEMQVRWAVKVFSGLTRLPLTQKMLEVTETERRLNLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQY8</accession>
    <name>A0A3B5KQY8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQY8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000295"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000302.1">
        <property type="protein sequence ID" value="ENSXCOP00000000295.1"/>
        <property type="gene ID" value="ENSXCOG00000000267.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001511"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045179">
        <property type="term" value="C:apical cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008093">
        <property type="term" value="F:cytoskeletal anchor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045176">
        <property type="term" value="P:apical protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008356">
        <property type="term" value="P:asymmetric cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009786">
        <property type="term" value="P:regulation of asymmetric cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.200.10">
        <property type="entry name" value="Inscuteable LGN-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045789">
        <property type="entry name" value="Insc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031938">
        <property type="entry name" value="INSC_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038205">
        <property type="entry name" value="INSC_LBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039921">
        <property type="entry name" value="Inscuteable"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21386">
        <property type="entry name" value="INSCUTEABLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21386:SF0">
        <property type="entry name" value="PROTEIN INSCUTEABLE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19427">
        <property type="entry name" value="Insc_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16748">
        <property type="entry name" value="INSC_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein inscuteable homologue LGN-binding" evidence="1">
        <location>
            <begin position="25"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Protein inscuteable homologue C-terminal" evidence="2">
        <location>
            <begin position="88"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Protein inscuteable homologue C-terminal" evidence="2">
        <location>
            <begin position="158"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="477" mass="52615" checksum="B909CD1DFFBA865D" modified="2018-12-05" version="1">MLFVFFNASTNIFIFSPSSTRMQTLQVDSVQRWMEDLRHMTEVECMCVLQAKPIGVDEDSHQGELIVTSGIGAGDHVARNNLQTLLRRALVVSTELGKMFQRLEKGRWQRVHSTAVRANCHVRSLVHEYSAARTSRYVIILMSCRSLSATRMLSVELLTTVFLDGCRLNSVFHCIQEGAVVALFKICRQDRFRDLYAHALRTVASICCVEEGINQLDKVDGILCLADILTDESSVEAARAEAAAVVAQITSPHHTSTQHLASFLESMHDIVTALIKLCESASCGEVFLLASAALANITFFDSMACEILLQLNAIHILLQACKDRQRVDTPYSKDQVVTILANLSVLESCASEVLQEQGIERLLLLLGEKPSSSNPSEVAACERVQQKAAVTLARLSRDPDVAHTAIQLKAVPRLIELCRSPTERNNSDSVLVACLAALRRLAAGCPDSIKAADHHQLIKPRLVDSFLLCSNMEESFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRL2</accession>
    <name>A0A3B5KRL2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome c oxidase assembly factor 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027899932.1">
        <property type="nucleotide sequence ID" value="XM_028044131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRL2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000281"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000288.1">
        <property type="protein sequence ID" value="ENSXCOP00000000281.1"/>
        <property type="gene ID" value="ENSXCOG00000000241.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114161067"/>
    <dbReference type="KEGG" id="xco:114161067"/>
    <dbReference type="CTD" id="388753"/>
    <dbReference type="GeneTree" id="ENSGT00390000004094"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042775">
        <property type="term" value="P:mitochondrial ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008535">
        <property type="term" value="P:respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.140">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042289">
        <property type="entry name" value="COA6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048280">
        <property type="entry name" value="COX6B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036549">
        <property type="entry name" value="CX6/COA6-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46690">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46690:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02297">
        <property type="entry name" value="COX6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47694">
        <property type="entry name" value="Cytochrome c oxidase subunit h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="9319" checksum="DD58E6FF14DCA2E5" modified="2018-12-05" version="1">MAAPNSAQRKACWDARDQLWQCLDDNNDKIQSCQQVQGDFEAKCPAQWVKYFTKRRDFLKYKEKMQTEGFTPAEGPQVNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KUC9</accession>
    <name>A0A3B5KUC9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Protocadherin 2 alpha a 15</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSXCOT00000003763.1">
        <property type="protein sequence ID" value="ENSXCOP00000003723.1"/>
        <property type="gene ID" value="ENSXCOG00000002910.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165118"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF290">
        <property type="entry name" value="PROTOCADHERIN 2 ALPHA A 15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017179613" evidence="13">
        <location>
            <begin position="31"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="28"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="135"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="244"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="352"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="462"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="535"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="644"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="752"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="858"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="982"/>
            <end position="1081"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1293" mass="142506" checksum="5DB840B86105D7C0" modified="2018-12-05" version="1">MERGKFEARLGKGCITRFAVAMLLLSATSAQLRYSISEEVNDGTAVGNLVKDLGLDKSTLKSRKYRVVSGGADPLFYVNESDGVLYVTRRIDREEVCAQSSVCLINVKTVLEAPLEVHNIAVEVLDINDHSPNFSEAEKILEISESVLPGARFQLKPARDLDSGQFSIQQYKLSQDDHFRLEVRDKEDDGKIPILVLKKSLDREAARSHSLLLTALDGGKPPKSGDMKIRVNVLDVNDNSPVFSMDVYSATLMENSPLGNTVVQVNATDLDDGQNGEIFFSFGNSVSSNIFSIFDINKSTGQITVKGHVDYEQKEKYEIEIQASDKGLAPLTAEKNVIIKIIDVNDNAPEIEITSFSSSIPEDSRPGTTVALISVNDLDSGLNGKVICSIGEEAPFSLLPSMQHNMFSLVTKSPLDRENTSHYELTITAKDSGQPSLSSVKTINVEVSDVNDNYPEFLRSPYTFYITEGNELGDSLFHLSDNGVLHISRKIDREEVCDQTASCIINIKTVLENPLEVHYVTIEVLDVNDHSPTFPKNETHLEISESALSGARFQLQDARDPDSGLFSVQQYKLSQNDHFRLEIKDRGQDGKIPVLFLHKPLDREVARSHRLLLTAVDGGKPPKSGTMGIVINVLDVNDNMPVFTRESYTVELNENSPVGTTVIQVNATDKDEGLNGEVVYSFGDNVNNKLRKLFEIDTITGAITVKGLLDYEAKDRYEIDIKASDKGPVPLSTEKSVIIAVVDLNDNAPEIEVTSLSSAIPEDSKPGRTVALISVSDKDSGVNGKVICSIIDDVPFTLTPSLQENMFSIVTKSLLDREHKSKYEVTIIAKDAGEPALSVQKTISVTVSDVNDNNPEFSTNPYNFYVTENNVAGASLFSLNAFDYDEGDNAHISYKIPRSGAAHKTVTTFLNINSETGTIVALKSFDFETLKTFQFQVVATDSGTPSLSSNVTVNVFILDQNDNAPVILYPLSSNGSAEGVEEIPRNVNAGHLVTKVRAYDADIGYSGWLLFSLQEVTDHSLFGLDRYTGQIRTLRSFTETDEAEHKLVILVKDNGNVSLSATATVIVKLVEPKEAFAASDVKSAANDDEGNDVTFYLMITLGAVSVLFIISIIVLIAMQCSKSTDYSSKYLQETNYDGTLCHSIQYRSGDKRYMLVGPRMSIGSTIVPGSHANTLVLPDRRRTCLLNSLLYRNVILHQNQPLRARQTTSDFLFIHPAHRHNVYDPAIHFLNTFVPCGAGAYLQLTFRVTDWTGRQSVTGLPVYSFVKHFFVYENKSVYCLCKNLRSSSKATKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KV54</accession>
    <name>A0A3B5KV54_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Choline dehydrogenase</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KV54"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004003"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004047.1">
        <property type="protein sequence ID" value="ENSXCOP00000004003.1"/>
        <property type="gene ID" value="ENSXCOG00000003138.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063260"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008812">
        <property type="term" value="F:choline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF147">
        <property type="entry name" value="CHOLINE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00623">
        <property type="entry name" value="GMC_OXRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0274">FAD</keyword>
    <keyword evidence="3 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="6">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="7">
        <location>
            <begin position="315"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="418" mass="47228" checksum="C560ED33F2F068A8" modified="2018-12-05" version="1">MGQAGAQRLVTGTWRRFMKDGRCFLDALFQKKWPNIRDFFLTSPNASSSPASNQKTPSYSYVVIGAGSAGCVLANRLTEDAQESVCLLEAGPKDKWLGSSRLSWKIHMPAALTYNLCDDKYNWFYHTLPQAQMDNRVLYWPRGRVWGGSSSLNAMVYIRGHAEDYNRWQREGAEGWDYEHCLPYFRKAQCHELGENRYRGGSGPLHVSRGKTNHPLHKAFIEAGQQAGYPFTDDMNGYQQEGVGWMDMTVYKGKRWSSASAYLRPALGRPNLKTEVHCMTTKILFDGKRAVGVEYVKNGEKKRVFADKEVILSGGAINSPQLLMLSGVGNADELKDLGIPVIQHLPGVGNNLQDHLELYVQQQCTQPITLYKAQKPFHMIKIGLEWLTLFTGNQIHLANVFIHPDVFTVLSHYNYAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KV96</accession>
    <name>A0A3B5KV96_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Latent transforming growth factor beta binding protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KV96"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001925"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001953.1">
        <property type="protein sequence ID" value="ENSXCOP00000001925.1"/>
        <property type="gene ID" value="ENSXCOG00000001550.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155823"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000046">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 1 isoform x2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000056">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.290.10">
        <property type="entry name" value="TGF-beta binding (TB) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017878">
        <property type="entry name" value="TB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036773">
        <property type="entry name" value="TB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052080">
        <property type="entry name" value="vWF_C/EGF_Fibrillin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333:SF4">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47333">
        <property type="entry name" value="VON WILLEBRAND FACTOR C AND EGF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00683">
        <property type="entry name" value="TB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57581">
        <property type="entry name" value="TB module/8-cys domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51364">
        <property type="entry name" value="TB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 8" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="TB" evidence="10">
        <location>
            <begin position="36"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="212"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="30080" checksum="EA02B2E3965B4D8E" modified="2018-12-05" version="1">MFVGLTTRPCFVHPWIICFRCDSPESQSSDQDVNANICWRRLEGNNLCAEPIQELRTTFTECCCLYGVAWGQCALCPRKTSVDYASMCNLPWTEGADSLRERIGYEYGVEEPELSPYWDNYGGGIPESVPIFTDSDYNNPQPPFRVPVLRPGGSQPQPLDQRRYDSFEGLQAEECGILNGCDNGRCVRVREGYTCDCFDGYELNLDKMACIDINECEDISDKVPLCQNGQCINTEGSYKCTCLPGFVASAKPHKCISAIPESTFRGAEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KVM2</accession>
    <name>A0A3B5KVM2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KVM2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002060"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002090.1">
        <property type="protein sequence ID" value="ENSXCOP00000002060.1"/>
        <property type="gene ID" value="ENSXCOG00000001651.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286954"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043473">
        <property type="term" value="P:pigmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000001">
        <property type="entry name" value="Gap junction alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF39">
        <property type="entry name" value="GAP JUNCTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0303">Gap junction</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="12">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="13">
        <location>
            <begin position="154"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="358" mass="40509" checksum="5C91B4F99ED13FC6" modified="2018-12-05" version="1">MSDWSFLEHLLEEGQEYSTAIGRIWLTVLFLFRILVLGTAAESAWDDEQADFVCNTKQPGCTSVCYDKAFPISHFRYFVLQVIFVSTPSIFYYGYVAIRGGKDPKAQEEIAEQKEKENCGGKRIAPKKASAEAPKLKGRLLRAYAFSILLKVFLEAGFIAGLWFLYDGFFIAAKFECTRFPCPHTVDCFVSRPTEKTIFTVYAQVIAGISLLLNLIELIHLLSLAFSYRLEKWDEDCLPLREQVQVTPALRMETPQVYNTGRDISIPTKGREAKLPGNPCESYAETVIEVNWDAGETGDNLLPSYMNCMKSTGKASSKRSYLVSFPKHKKQTPGGHFYLTCASQHFVEPPFHNRHSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KWR4</accession>
    <name>A0A3B5KWR4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Beta-catenin-like protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Nuclear-associated protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. Participates in AID/AICDA-mediated somatic hypermutation (SHM) and class-switch recombination (CSR), 2 processes resulting in the production of high-affinity, mutated isotype-switched antibodies.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts directly with CWC15 and CDC5L in the complex. Interacts with AICDA; the interaction is important for the antibody diversification activity of AICDA. Interacts with PRPF31 (via its NLS). Interacts (via its N-terminal NLS) with KPNA1 and KPNA2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KWR4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000014"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000014.1">
        <property type="protein sequence ID" value="ENSXCOP00000000014.1"/>
        <property type="gene ID" value="ENSXCOG00000000014.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006931"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001136">
        <property type="entry name" value="Beta-catenin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039678">
        <property type="entry name" value="CTNNBL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013180">
        <property type="entry name" value="CTNNBL1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14978:SF0">
        <property type="entry name" value="BETA-CATENIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14978">
        <property type="entry name" value="BETA-CATENIN-LIKE PROTEIN 1 NUCLEAR ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08216">
        <property type="entry name" value="CTNNBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01156">
        <property type="entry name" value="DUF1716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta-catenin-like protein 1 N-terminal" evidence="11">
        <location>
            <begin position="68"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00083862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="551" mass="62158" checksum="F4DA4D6116E1FAAD" modified="2018-12-05" version="1">NVTLNVVLLQPDRGAKRPRGEDGGAAGGDDNSRGGKQQKGVGPREAARFREVGGGEGGAEMKETEEPTGDRKMILEKLMDQDGEEPEAEPVDESSVKKMILTFEKRSYKNQELRIKFPDNPEKFMESELDLNDIIQEMHVIATMPDLYHLLVELNAVHSLLGLLSHENTDILLNTVVDLLQELTDIDTLHESEEGAEVLIDALVKGQVVALLVQNMERLDEQVKEEADGIYNTLAITENMAEFRPGLCTEAAQQGLMQWLLKRIKAKMPFDANKLYCSEILAILLQNNDTTRELLGEMDGIDVLLQQLSVFKRHNPNTAEEQEMMENLFDALCSCLMLSSNRDRFLKGEGLQLMNLMLREKKMSRTSALKVLDHAMIGPEGADNCHKFVDVLGLRTIFPLFMKTPKKMKKTGTSEKEHEEHVCSVIASMLRNLKSQQRTRLLNKFTENDCEKVDRLMELYFKYLEAVQQADKRIEGEKHVRAIFLSSLLTDLVFLMKRFISVSVEIRQFDGGKVPRQRIGVHKVLKELLLSSNVERPGESHRNTVNTGGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KY19</accession>
    <name>A0A3B5KY19_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Solute carrier family 2, facilitated glucose transporter member 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Fructose transporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Glucose transporter type 5, small intestine</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose(out) = D-fructose(in)</text>
            <dbReference type="Rhea" id="RHEA:60372"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <location evidence="2">Sarcolemma</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KY19"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002531"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002565.1">
        <property type="protein sequence ID" value="ENSXCOP00000002531.1"/>
        <property type="gene ID" value="ENSXCOG00000002021.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166161"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005353">
        <property type="term" value="F:fructose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046323">
        <property type="term" value="P:D-glucose import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070837">
        <property type="term" value="P:dehydroascorbic acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990539">
        <property type="term" value="P:fructose import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:001511">
        <property type="entry name" value="Solute carrier family 2, facilitated glucose transporter member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF22">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="9 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="386"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="422"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="16">
        <location>
            <begin position="26"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="516" mass="56169" checksum="8613A4AB341BCC10" modified="2018-12-05" version="1">MSSTGTNETEERGNSAGGARTLALTVFFAAIGGTFQYGYNISIINAPTSYIQNFINDTYMERWGTGLDTPQVTLLWTFIVSAFSLGGLLGALLAGPMAVRFGRKVSLLLNNSFLLVGAVLVLTCKVAKSFEMIVLARFLVGINSGVSMNIQPMYFGESAPKHLRGAVAFSSAVFTAFGIFFGQVVGLTELLGSEPLWPYLLASNALPALIQLVTLPWFPESPRYLLIDRGDREACVQALGRLRGGKVPPLEMKEMLQEQQMSAALNSGSSAPAKTPWSLFKDPDLRLQLRTVMTASSAMMLCGNDSIYFYAYYIFLEAGIPPEKIQYITIGTGASEFTASLLSNVLIERVGRRYLLIGGYSLMVCWTIVFTVALTLQSHGVTGMPYLSMACVFAYILSFGLGPAGVTGILPAEIFDQTARPAAYMVAGSFMWISLFLVGMLFPFIVNGLGNYCFLPFLVVCVVSAVVLGRTMPETKGKTLAEITAEFDRKNGRAFQVEEPEQYQPDVMHFLVPLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L0D9</accession>
    <name>A0A3B5L0D9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein RIC1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000003780"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003820.1">
        <property type="protein sequence ID" value="ENSXCOP00000003780.1"/>
        <property type="gene ID" value="ENSXCOG00000002948.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002955"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034066">
        <property type="term" value="C:Ric1-Rgp1 guanyl-nucleotide exchange factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040096">
        <property type="entry name" value="Ric1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009771">
        <property type="entry name" value="RIC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22746:SF10">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR SUBUNIT RIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22746">
        <property type="entry name" value="RAB6A-GEF COMPLEX PARTNER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25440">
        <property type="entry name" value="Beta-prop_RIC1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07064">
        <property type="entry name" value="RIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RIC1 C-terminal alpha solenoid region" evidence="5">
        <location>
            <begin position="795"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="448"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1336"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1353"/>
            <end position="1369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1379"/>
            <end position="1393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1399" mass="155740" checksum="F4B03D4BDA5D1739" modified="2018-12-05" version="1">MYFLTGWPRRLLCPLRSAEEPFHIQPSSQRLYFALLSETQLSVWFSRPSVLIVSYIESAKAAAQFGYYQKAEWKPDDSMVAVATANGYILLFDVLGGGEDKYLYEPVYPKGSPRVKVTPGYKEEQCAPALSLEMKKPTLSLNHCLDFLLSEDLLVCTADGYLHVLHWDGLGSNGRKAICLTTIPFSLDLQSARGGPSLDLEGVYIRSMEYCLTLDGFAVVLSDGRLGFITPLSSSITADQLQGVWAADVTDGTCVAVNNKYRLMTFGCASGSVLVYMIDTTTGSMQLSHKLELTPKHYPDIYNKTGGVKLICWSPDYSVVMVTWECGGLSLWSVFGAHLICTLGEDFAHHSDGTKKDPLKISSMSWGAEGYHLWVIPSRQERRKQEDEEQDVEMISPPHPSLQAGILQFHFIKSALTVNPCTSNQEQVLLHGEDRLYLTCGDPRQIHSTSDAHTNLHPRDGSPLHHPPNPDSSLSQGLSTLLGHKHWHVVQIHSTYLESNWPIREQNMTVTGGLAWWKDFVVVSCYNFTDQQEQLRLYQRSSNLDNAFASVTKLHSDTLLLNVFRDMVILFRADCSICLYSIEKRNDSPTPSVSVELLQEVSMSRYIPHPALVVSVTLTSVRTETGISLKAPQQACMAESIMLNLAGQLIMLQRDRSGPQVRDKETPAVNKKLLPFCPPVVLAQCVENVWTTCRSNKKKRHLLEALWLSCGEAGMKVWLPLFPRDHRKPHSFLSRRIMLPFHINIYPLAVLFEDALVLGATNETVLYDGLQGSSEPLEALFPYCTVERTSQIYLHHILRQLLVRNLGEQALMLAQSCAALPYFPHVLELMVHVVLEEEATSREPIPDPLLPTVAKFITEFPLFLQTIVHCARKTEYALWNYLFAAVGNPKDLFEECLMAQDLDTAASYLIILQNMEVPAVSRQHATLLFNTALEQGKWDLCRHMIRFLKAIGSGEMESPPPTPSTQEPSSTGGFEFFRNRSISLSQSADSITTGKFNLQKTFSMPSGSSAKGRDVECAENMYIDMMLWRHARHLLEQVRLRDLGCFSAQLGFELIGWLCRERNRVARVDDFVSALKKLHKDFLWPFPVIPVGSLSSPLKNELLSTQLLKSQSADSLLNSNMDTAPPSTATANHTWMDGLEQSTKDMDTASSAHSNQNSPQTHDAFLSLLTNKVEEYSVGSATDLTETSSVVDGDWTMVDENSSTLSLSQAELEHISMELANKGPHKSQVQLRYLLHVFMEAGCLEWCVVIGLILRDANVIKQVISFLDSPEVPPETSQSLRNGLLAVDAWASTDCLGYRPFLNLIQPQLQQLLDSTVEQVQPEAFQPASQSCKLAASEGPGGVAAPRPEDSRGGAAPLGLALPALEPAGVFSRPPPEDSPPEQTEEQREEEGNYDCTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1A3</accession>
    <name>A0A3B5L1A3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA methyltransferase 10 homolog C</fullName>
            <ecNumber evidence="2">2.1.1.218</ecNumber>
            <ecNumber evidence="3">2.1.1.221</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Mitochondrial ribonuclease P protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">RNA (guanine-9-)-methyltransferase domain-containing protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">mRNA methyladenosine-N(1)-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">tRNA (adenine(9)-N(1))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">tRNA (guanine(9)-N(1))-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>adenosine(9) in tRNA + S-adenosyl-L-methionine = N(1)-methyladenosine(9) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43148"/>
            <dbReference type="Rhea" id="RHEA-COMP:10363"/>
            <dbReference type="Rhea" id="RHEA-COMP:10364"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74491"/>
            <dbReference type="EC" id="2.1.1.218"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>an adenosine in mRNA + S-adenosyl-L-methionine = an N(1)-methyladenosine in mRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55392"/>
            <dbReference type="Rhea" id="RHEA-COMP:12414"/>
            <dbReference type="Rhea" id="RHEA-COMP:12415"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74491"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>guanosine(9) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43156"/>
            <dbReference type="Rhea" id="RHEA-COMP:10367"/>
            <dbReference type="Rhea" id="RHEA-COMP:10368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.221"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.218" evidence="2"/>
    <dbReference type="EC" id="2.1.1.221" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1A3"/>
    <dbReference type="SMR" id="A0A3B5L1A3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003666"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003705.1">
        <property type="protein sequence ID" value="ENSXCOP00000003666.1"/>
        <property type="gene ID" value="ENSXCOG00000002876.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063169"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160106">
        <property type="term" value="F:tRNA (adenine(9)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052905">
        <property type="term" value="F:tRNA (guanosine(9)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097745">
        <property type="term" value="P:mitochondrial tRNA 5'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070131">
        <property type="term" value="P:positive regulation of mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18102">
        <property type="entry name" value="Trm10_MRRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.30:FF:000003">
        <property type="entry name" value="tRNA methyltransferase 10C, mitochondrial RNase P subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038459">
        <property type="entry name" value="MT_TRM10-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025812">
        <property type="entry name" value="Trm10_C_MTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007356">
        <property type="entry name" value="tRNA_m1G_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563">
        <property type="entry name" value="TRNA (GUANINE-9-) METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563:SF5">
        <property type="entry name" value="TRNA METHYLTRANSFERASE 10 HOMOLOG C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="21">
        <location>
            <begin position="88"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="31"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="284"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="292"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="308" mass="36280" checksum="FB2EBDD32AE03779" modified="2018-12-05" version="1">MTEEDLQTLAGLTTKSSRKKYLKYLAVKEGHKQARKEKQQKKKADKEASMKQRKELENSEEEEGDQDGHRLKNTMFLQFWDRTLDKLLGWRTAQAMRFGQPIVFDLSYASNMSRREIENTVSQLMEVEGWNRRATEPFHLHFCNLQPDSLYKKELVKRYGAETWDRLLITSTEQHYIDMFPREQLVYLTADSPNVLRSFDHSKVYIIGALVDKSIQSGLSLANAKRLKLATARLPLDEYLHWEMGAKNLTLDQMIRIMLTIKDSGKWEEALQFVPKRKHDGFYQRQTQTEVSNDRPRIVKKHQENRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L488</accession>
    <name>A0A3B5L488_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Protein phosphatase 1L</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Protein phosphatase 1-like</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Protein phosphatase 2C isoform epsilon</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Acts as a suppressor of the SAPK signaling pathways by associating with and dephosphorylating MAP3K7/TAK1 and MAP3K5, and by attenuating the association between MAP3K7/TAK1 and MAP2K4 or MAP2K6.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="RefSeq" id="XP_027875739.1">
        <property type="nucleotide sequence ID" value="XM_028019938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L488"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002579"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002614.1">
        <property type="protein sequence ID" value="ENSXCOP00000002579.1"/>
        <property type="gene ID" value="ENSXCOG00000002064.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114146127"/>
    <dbReference type="KEGG" id="xco:114146127"/>
    <dbReference type="GeneTree" id="ENSGT00940000157030"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000017">
        <property type="entry name" value="phosphatase 1L isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 20" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="21">
        <location>
            <begin position="92"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00070219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00076684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00081433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="363" mass="40647" checksum="8C9D1FD3D9FFB4EC" modified="2018-12-05" version="1">MIGDTMTLLSLLGRIMRYFLLRPETLFLLCISLALWSYFFHTDEVKTIVKSSRDAVKMVKGKVAEILQNDRFGGLSVLDAEFSKTWAFKNNNVAVYSIQGRRDHMEDRFEVLTDITNKSHPSIFGIFDGHGGEAAADYAKAHLPESLRQQLQALEREKRESAVSYASILEQRILTVDREMLDKLSANHDEAGTTCLMALLSDRELTVANVGDSRGVLCDKDGNAVALSHDHKPYQLKERKRIKRAGGFISFNGSWRVQGILAMSRSLGDYPLKNLNVVIPDPDIMTFDLDKLQPEFMILASDGLWDAFSNEEAVRFVRERLDEPHFGAKSIVLQSFYRGCPDNITVMVVKFKSGAGGSSKAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4V2</accession>
    <name>A0A3B5L4V2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphatidylserine decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.65</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine. May be involved in lipid droplet biogenesis at the endoplasmic reticulum membrane.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13">Phospholipid metabolism; phosphatidylethanolamine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.1.1.65" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L4V2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005425"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005487.1">
        <property type="protein sequence ID" value="ENSXCOP00000005425.1"/>
        <property type="gene ID" value="ENSXCOG00000004237.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013484"/>
    <dbReference type="UniPathway" id="UPA00558"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033177">
        <property type="entry name" value="PSD-B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00163">
        <property type="entry name" value="PS_decarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067:SF6">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE PROENZYME, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="12" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="259" mass="28713" checksum="2F19774D5E3DE85C" modified="2018-12-05" version="1">MTGLEAAVEDLHHYKNLGEFFRRRLKPAVRPVCAASCLTSPADGRILHFGQVQNSQVEQVKGVTYSLENFLGPPSRPSPDPASFQDRLLSSPDRALFHVVVYLAPGDYHCFHSPADWRVELRRHFPGSLMSVNPGVARWVKELFCLNERVVLSGQWRHGFFSLTAVGATNVGSIRVYFDQELRTNAPRYSKGSFQDRSYVATSDQVKASEGGVALCKGEPVGEFNLGSTIVLLFEAPADFTFDLQPGQRIRMGEPLGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6H7</accession>
    <name>A0A3B5L6H7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6H7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007913"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008011.1">
        <property type="protein sequence ID" value="ENSXCOP00000007913.1"/>
        <property type="gene ID" value="ENSXCOG00000006075.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287090"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF111">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Immunoglobulin V-set" evidence="8">
        <location>
            <begin position="59"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="172" mass="19557" checksum="33E197FE6C627DF7" modified="2018-12-05" version="1">MQITVILLCSHSKYDIKSSSTWTPFKLCSLTLSVFHSCLFTFVILELNLFVSGWICESLTVDVHPGEDITLFCSNISPSPTQTDWFRVVHGTKPSCISSMYGAEGEPSYCDGFHREKFIMSSNKTVFFLQIKQVDLMDSGLYFCGFYVKKHTVIGRATERNNRPKNIIHTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6P8</accession>
    <name>A0A3B5L6P8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GHMP kinase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6P8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007983"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008081.1">
        <property type="protein sequence ID" value="ENSXCOP00000007983.1"/>
        <property type="gene ID" value="ENSXCOG00000006129.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00540000072704"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047940">
        <property type="term" value="F:glucuronokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053034">
        <property type="entry name" value="Glucuronokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38710">
        <property type="entry name" value="WITH PUTATIVE URIDYL PYROPHOSPHORYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38710:SF1">
        <property type="entry name" value="WITH PUTATIVE URIDYL PYROPHOSPHORYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="1">
        <location>
            <begin position="398"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="494" mass="55993" checksum="E2D7AAE10C81FA3D" modified="2025-10-08" version="2">MLCSILSFETNITPGELIIYYELEEGERSSSRGIVEVCPETHRITRFLEKPEEGRTASRLASVVFYCLRRDTLSCMSNFLSLYTRATERTFGLFWEWLINEKKLDVFGMKLPTGFQLIGQVGLSDYTKWLTHYSTKKQDNPAQPITCRSYARVGLMGNPSDGFNGKTIAMTISNFWAEVTLVESQTLVTGLPHPLNDPTEFGSLQDLFCISRREGYLGGLRLLQATCKKFYQFCSKRGIALTKQNFTLKYDTNIPRQVNKYFPLSLYHHSAIVSATLKCLMKFYNITDNDLPKSIRANFILSVETDELFITAGLQDRVVQVYEGLVYMDFSKKLMDEQGYGNYASMDMSDLPPFWLAYLSDPSDSGRIHSNIRQRWLSGEPVVVEAMKTFAELTDQARSAIKDRDWTRLAQLMDQNFELRRSIYTDDCLGPGNLRMVQLARQFGSAVKLPGSGGAVVGLCPDQARLVEMRQAFQEAGCVFCVISPYIPSARGQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L702</accession>
    <name>A0A3B5L702_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L702"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003559"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003598.1">
        <property type="protein sequence ID" value="ENSXCOP00000003559.1"/>
        <property type="gene ID" value="ENSXCOG00000002803.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161002"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000049">
        <property type="entry name" value="Solute carrier family 25 member 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="99"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="195"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="288" mass="31327" checksum="84BA242EC6BA0E0A" modified="2018-12-05" version="1">MADATTSPLMAFAGAVGIAVGHPLDTVKVRLQAKSVYKGIFDCVTKTYLNEGFHGFFKGMSFPVLTTGLVNSIVFGSYSNALDYLSQSDRRLTSHRKQVSSLQVFTAGCFSGMVQVLVCAPIDLVKVRLQGQTTAKRYRRPIHCVAVILQQEGVRGLYRGGAALALRDVPCYGLYFLPYELIRKYYHNIDNTSSTGTFAILMAGGVAGVVTWACATPMDVVKARLQMSGAGGQQYSGVLHCMRVSLKEEGVRVFFKGLLLNSVRAFPVNAVTFLTYESLLKSFCPSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L807</accession>
    <name>A0A3B5L807_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha-1A adrenergic receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Alpha-1A adrenoreceptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L807"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006952"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007040.1">
        <property type="protein sequence ID" value="ENSXCOP00000006952.1"/>
        <property type="gene ID" value="ENSXCOG00000005378.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166031"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004937">
        <property type="term" value="F:alpha1-adrenergic receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002233">
        <property type="entry name" value="ADR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF16">
        <property type="entry name" value="ALPHA-1A ADRENERGIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01103">
        <property type="entry name" value="ADRENERGICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="34"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="192"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="51"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="370"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="374"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="400" mass="43623" checksum="F6F5B9FD6184C335" modified="2025-10-08" version="2">MKNLSSPKVGVLSPDDFCPNCSSSAQSEVHLAKAVALGAVLVVFLLFGVLGNILVILSVLLHHQWRSVTHYFIANLAAADLLLSSAVLPFSAASEALGRWVFGRSFCNVWAALDVLCCTASILSLCVISIDRYLAVSYPLRYPAIATGRRGLTAVAALWGVSAAISVGPLFGWKEPDPEDETDCRITQEPGYALFSALGSFYIPLAIILAMYCQVYTVARRETKSIRESTKGEEVEMGGVTLRMHRGNAAAQAGTHKDGEEAGTHKRSSFPLPKLLRFSGEEKAARTLGIVVGCFILCWLPFFLVLPIVWFVTENPPETIFKITFWLGYLNSCINPIIYPCFSQEFKKAFHNMLRGRCVRTAVPVTKQGHVAAHSSSPGPTSNASVSSVQNRYCHKPKHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8I3</accession>
    <name>A0A3B5L8I3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Prickle homolog 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prickle / espinas / testin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8I3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006760"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006843.1">
        <property type="protein sequence ID" value="ENSXCOP00000006760.1"/>
        <property type="gene ID" value="ENSXCOG00000005231.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153629"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09415">
        <property type="entry name" value="LIM1_Prickle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09418">
        <property type="entry name" value="LIM2_Prickle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09420">
        <property type="entry name" value="LIM3_Prickle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09827">
        <property type="entry name" value="PET_Prickle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000022">
        <property type="entry name" value="prickle-like protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000035">
        <property type="entry name" value="prickle-like protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000005">
        <property type="entry name" value="Testin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033725">
        <property type="entry name" value="LIM1_prickle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033726">
        <property type="entry name" value="LIM2_prickle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033727">
        <property type="entry name" value="LIM3_prickle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010442">
        <property type="entry name" value="PET_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033723">
        <property type="entry name" value="PET_prickle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047120">
        <property type="entry name" value="Pk/Esn/Tes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24211">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24211:SF18">
        <property type="entry name" value="PRICKLE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06297">
        <property type="entry name" value="PET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51303">
        <property type="entry name" value="PET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PET" evidence="9">
        <location>
            <begin position="18"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="128"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="8">
        <location>
            <begin position="194"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="314"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="508"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="683"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="346"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="384"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="434"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="516"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="552"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="695"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="884" mass="99894" checksum="0C0EB9E639DAC81B" modified="2018-12-05" version="1">VVTAMSLEMEKTITKLMYDFQRNSTSDDDSGCALEEYAWVPPGLNPEQVHQYYNSLPEEKVPYINSPGEKYRIKQLLHQLPPHDNEVRYCNALDEEEKRELKIFSNQRKKDNLGRGNVRPFPLTVTGMVCEKCGGQINGGEIVVFAARAGHGKCWHPACFVCCTCEELLVDLIYFLQDGKIYCGRHHSERLKPRCCACDELIFADECTEAEGRHWHMKHFCCYECETTLGGQRYIMKDGRPHCCNCFESLYAEYCDACGEHIGIDQGQMTYDGQHWHATEECFCCARCKRSLLGRPFLPKQGQIFCSRSCSAGQDPEESDSSDSAFQSARSRESRHSTKIGKKERRNAEQDRRSTEPRQSAPPPMPDRLSAEIDPLSVPMDRLSLSSSQTPSRTPNRTPMWMSRDEPYIPASYEGPKRDPSPTPASIHLLGQCNARQGYNPNTNAHPPAQSPANPGKRPDSWGKEQGNAKRTPMAALRGHSFNENWIHHSQDEFRPNKLRTQMSFNEMSSQNQGFSDKRSTSLHGFQRDGRPPLTRRNHITAMSFNEPLTPLEQTPRGSMDSITMSNTAGNSLDGATKRQEHLSRFSMPDLSKDSGVNVSEKSNMGTLSSSVQFHSTESLSSSRPYNNNMYTPLRVGYPLQYWEGPQDIGCDGKGRVGLMGSSGNLRMAPMSDRMPRRRINAQESISQQQQPQPRPHKHHRGTNGHHRSGRHHKRSRRSRSDNALHLVADRPTQMVELPYRRVQEDYDRFPSGQAARELYGVDSSGHRQMPHRPCPRTTSDLTLQNAGWQPVGLGGPCWNDGYMEAADPWCSSCSSSSESEADEGYYLSDPIPRPVQLCYINNEELRHRYSPSGIGAHHGPLHGPIHGQLHSRQRRKSKNCIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8J0</accession>
    <name>A0A3B5L8J0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pleckstrin homology domain containing, family H (with MyTH4 domain) member 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000008613"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008719.1">
        <property type="protein sequence ID" value="ENSXCOP00000008613.1"/>
        <property type="gene ID" value="ENSXCOG00000006587.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157675"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030835">
        <property type="term" value="P:negative regulation of actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13282">
        <property type="entry name" value="PH1_PLEKHH1_PLEKHH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000286">
        <property type="entry name" value="PH-protein kinase domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.530:FF:000001">
        <property type="entry name" value="Pleckstrin homology domain-containing family H member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.530">
        <property type="entry name" value="MyTH4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000857">
        <property type="entry name" value="MyTH4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038185">
        <property type="entry name" value="MyTH4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22903:SF3">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY H MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22903">
        <property type="entry name" value="PLEKHH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00784">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00139">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51016">
        <property type="entry name" value="MYTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="581"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="690"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="domain" description="MyTH4" evidence="7">
        <location>
            <begin position="834"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="domain" description="FERM" evidence="6">
        <location>
            <begin position="1000"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="190"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="230"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="343"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="545"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1323"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="88"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="232"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="354"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="448"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1344" mass="151150" checksum="BDCB115EB5254238" modified="2018-12-05" version="1">MAEGEEAMSQEDWKERCTVLEALLVKFRVQIVKIRELTADKIQQLETQVIDAEKRAFAANQQVKWMEEKLKAADGHLGDSEMRLFEQCQELQALVQEKEEVITQLEQQLEEQKQTRLQDAKSVEEKAAKIKEWVMLRLSEFEVENAALRETNKKQEAQILELCKPGEAQRLSSLTFGCFQVRGKNPQVLTGPAPCQRTLSTQEEMDTTRKSSHRGNSYFSIYLNKQEDTGLRKQQNTGGDVPEQNQSGLGISLQEDSNSPKKSPESASEGQEVCEGKGRVSGFSRPCSENYLTASDDSSSLFDDDMQRAERPVFGLVQLSDAAPADGLSRAEAKLEDCASEELNQRFQSQRLDSSSSSSEPTTPSPILTPALTPKRPNAPRDPTDNPASPKQPRLRTPAPLVKKHLSQPPVGSQAAHGQTRNALSMLRPLRPQETDLDLENSMETGRETPQSSPTSNQTSSESSSESPDCDASGPCKPPTPPLHRLPSWVRLQKHKILPSFFEVEPPAQPSRMHLVISNWEVKNKSLVFSSSRSVSVASMTSESDYAIPPDAYSTDTEFSEPEQKLPKTCSSASDNGKSEPMEKSGYLLKMVKTWKKTWKRRWFVLKDGELLYYKSPSDVIRKPQGQIEVNASSSITRGGGKQVLQIMTGKHVYYLKADSPNLLEEWLHVLQSVLRLKAASPLFTQPDIRPIMKGLLIKVKHGYSKRVSCALIGKTLYYFRSQEDKFPLGQIKLWEARVEEVAGSKESDDELKACGRSLEAAPFTIAVHPQEQSPTYLLIESRHEKDSWLYHLSVAAGTTVGKVGTEFEQLVGKLFQLDGDANSQIWRNPTLCFRKEALSSPLTTLPSQALQTEAVKLFKTCQLFINVAIDAPAIDYHVSLAQSALQVCLAHPELQNEFFCQLMKQTRMKQPQSHAGPLQGWQFLALCVGLFLPRHPFLWLLQVHLKRHGDSRTEVGKYAIYCQRSLERTQQKGERQARPSRMEILSILLRNPYHHSLPFSVPVHFLNNTYQVVSFDASTTVDEFQSRLNQDTGMRKTGLSGFSLYTDDPTGRELEHCLSGGIKICDILSKWEQASKEQHSGKSENTRTVRLTYKNRLYFSHQMRGESERERLLLAYQTNEAIVVGHFPVNKELALEMAALLAQVEFGDFERSLFASGSAQSKPNQSLKQVLDRFYPKHYRRTSSEDQQRQLMQRLSARWASLKGRGSSECVRIYLTVARKWPFFGAKLFQAEVRETKLLVTHPYKNLMTFGGCKEDFMLVVGQNIGKDKPTEKHLFAMDASKIREITLLISSYINSAHQQKAAAHHLSAPALMVAQPVSLKSKELRSKSPPALGRPSKAPTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8J4</accession>
    <name>A0A3B5L8J4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Forkhead box protein L2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027890144.1">
        <property type="nucleotide sequence ID" value="XM_028034343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8J4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006081"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006151.1">
        <property type="protein sequence ID" value="ENSXCOP00000006081.1"/>
        <property type="gene ID" value="ENSXCOG00000004707.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114154856"/>
    <dbReference type="KEGG" id="xco:114154856"/>
    <dbReference type="CTD" id="100004081"/>
    <dbReference type="GeneTree" id="ENSGT00940000162075"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20028">
        <property type="entry name" value="FH_FOXL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000016">
        <property type="entry name" value="Forkhead box protein I1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047515">
        <property type="entry name" value="FH_FOXL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF411">
        <property type="entry name" value="FORKHEAD BOX PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Fork-head" evidence="13">
        <location>
            <begin position="44"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="11">
        <location>
            <begin position="44"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="260" mass="29221" checksum="FCA8449E79DB5663" modified="2018-12-05" version="1">MAAEAKTLGTQEMQLLDISSSSPQTESKGSEETEYEDGTVQLEKPPYSYVALIAMAIKESQDKRRTLSGIYEYIISKFPYYEKNKKGWQNSIRHNLSLNECFIKVPRDSGADRKGNYWMLDPAFEDMFEKGNYRRRRRVRRPYRPASLPFLTGNPVEYPESLYLQPYVGGTWASGYPAPQVVSGSTRGLSIGGSYCPPPHFHPSVYGAYHPPVLVPHNGYPYGGVTQPVSPDGGTMSVPCPQFTSYSRQAESPLVYFSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8J8</accession>
    <name>A0A3B5L8J8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Monooxygenase, DBH-like 1, like</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the copper type II ascorbate-dependent monooxygenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8J8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006086"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006156.1">
        <property type="protein sequence ID" value="ENSXCOP00000006086.1"/>
        <property type="gene ID" value="ENSXCOG00000004712.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063085"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030667">
        <property type="term" value="C:secretory granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004500">
        <property type="term" value="F:dopamine beta-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042420">
        <property type="term" value="P:dopamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042421">
        <property type="term" value="P:norepinephrine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006589">
        <property type="term" value="P:octopamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09631">
        <property type="entry name" value="DOMON_DOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.230:FF:000001">
        <property type="entry name" value="Monooxygenase, DBH-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1210:FF:000001">
        <property type="entry name" value="Monooxygenase, DBH-like 1, like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1210">
        <property type="entry name" value="Cellobiose dehydrogenase, cytochrome domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.310">
        <property type="entry name" value="Copper type II, ascorbate-dependent monooxygenase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014784">
        <property type="entry name" value="Cu2_ascorb_mOase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000323">
        <property type="entry name" value="Cu2_ascorb_mOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036939">
        <property type="entry name" value="Cu2_ascorb_mOase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024548">
        <property type="entry name" value="Cu2_monoox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000945">
        <property type="entry name" value="DBH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045266">
        <property type="entry name" value="DOH_DOMON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008977">
        <property type="entry name" value="PHM/PNGase_F_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028460">
        <property type="entry name" value="Tbh/DBH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10157:SF41">
        <property type="entry name" value="DBH-LIKE MONOOXYGENASE PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10157">
        <property type="entry name" value="DOPAMINE BETA HYDROXYLASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03712">
        <property type="entry name" value="Cu2_monoox_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01082">
        <property type="entry name" value="Cu2_monooxygen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03351">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00767">
        <property type="entry name" value="DBMONOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00664">
        <property type="entry name" value="DoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49742">
        <property type="entry name" value="PHM/PNGase F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017465773" evidence="11">
        <location>
            <begin position="31"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="12">
        <location>
            <begin position="45"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="524" mass="59110" checksum="98DD404A68E6BBD6" modified="2018-12-05" version="1">AGVSLKGRDIMSAPLCFVSILLALMAGSRATDYNLPFMEYLDNGNLVHLKWGFDNVQGNITFQLTVNTTGWVGFGFSPRGDMDDADIVMGGLESNGSYFADYYSIGEKMPEKDEEQSYNLLSVTENDGQTIMTFQRPIETCDKKDFHITSIKLIYAYGATDNIKYHGTFRGTKEVNLLNYMPKTVPPDARYLSITVPSNKTYYHCKVMKLQDLPTKHHIYQIEPEIEHQEVVHHMLLYHCPSFVKEPYDKPCYMGEIGDACFGVVASWAVGGRAYELPENTGIPIGGTQSGTYYRLEIHYNNPQKKEGIIDSSGLRLYYTDKLRQHDVGILTTGVFPVSHMMYDIPPKAAEFHSYGICNTSFFHFHPQLVDPIPDLQVFSVLLHTHLAGRKVRVGHFRKGKQIDFLGVNENYDFDMQETVNLGSIKTIKPDDEIIVECIYNTSNRTQSTMMGLSTSDEMCLAFLFYYPAINITSCTSRPNTTLLSTTSTTLCVKITASKKICFNNFCYHCSTTHRFTDMALSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L984</accession>
    <name>A0A3B5L984_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NIPA like domain containing 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NIPA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L984"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006316"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006393.1">
        <property type="protein sequence ID" value="ENSXCOP00000006316.1"/>
        <property type="gene ID" value="ENSXCOG00000004870.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158233"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF14">
        <property type="entry name" value="NIPA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="381" mass="41745" checksum="66D899982BDAF8F3" modified="2018-12-05" version="1">LIGTLLAIFGSVIVSISLSIQKYSHVTLAGTKEQRTFYNTKTWWSGFVFICIGEGANFVSYAFAPIAVVAPLNAVSVLTSSILGFLFLREKSKAKDFAKNHGLAFLGYIFIIGGTYLFVSFGPNSHEKLQAENIVKHLIGWPVLLYMLLEIIAFCLLLYFYKQHNANYLVVVLLLVALLGSVTVITVKAVSGMLLLTLEGSMQLDYPIFSVMFVCMVASVIFQARFLSQACNLHDPSLAACVNYIFSTIFAVVAGELQLLRACKHDHNMSQNFSSFHPQVPFSTWSFRRKIHFLPHGLHQSCCGASSPSPRPDWSVRAAVGVSLVPPAHGSAGGVYAVTMPPMIIHGQQTPTHLTAPTKITASPIRLTQQSDGKILVSFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCA9</accession>
    <name>A0A3B5LCA9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCA9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008583.1">
        <property type="protein sequence ID" value="ENSXCOP00000008477.1"/>
        <property type="gene ID" value="ENSXCOG00000006498.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000203887"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="91" mass="10351" checksum="57BBFF8B957AC2DA" modified="2018-12-05" version="1">FQKVILEGIRVVSNLDSVPIAVVMLFSLIYGLNLANGADLRYTFELLPKIMMELVGGKLSNKALSLKNASMRHKFICYFLHIYLNCHYDIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCU9</accession>
    <name>A0A3B5LCU9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Acetylcholine receptor subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-1/CHRNB1 sub-subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCU9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008360"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008466.1">
        <property type="protein sequence ID" value="ENSXCOP00000008360.1"/>
        <property type="gene ID" value="ENSXCOG00000006405.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158661"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005892">
        <property type="term" value="C:acetylcholine-gated channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015464">
        <property type="term" value="F:acetylcholine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000026">
        <property type="entry name" value="Cholinergic receptor nicotinic beta 1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000031">
        <property type="entry name" value="Cholinergic receptor nicotinic beta 1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000012">
        <property type="entry name" value="Nicotinic acetylcholine receptor subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 21" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 21" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 21" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 21" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022257176" description="Acetylcholine receptor subunit beta" evidence="21">
        <location>
            <begin position="25"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="22">
        <location>
            <begin position="28"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="23">
        <location>
            <begin position="245"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00037951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00040199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="493" mass="57122" checksum="E289F795CC7C64B4" modified="2018-12-05" version="1">MRLSFNMDSFLIICCILCFTSTGASETERRLHEKLFQDYNVKVRPARHWKERVTVRVGMTLSQLIGLNEKNSEMTTNVFMNLNWTDYRLSWKPEEYDNIAVVRIPPNKVWRPDIYLINNHDGQFDVALYVNVLVYSNGMVSWLPPAIYRSSCSIEVAYFPFDWQNCSMVFRSYTYDASEVDLQVYLDENGTEIHEIVIDKNAFTENGEWAIRHKPSRKNVGDDLYEDITFYLIIERKPLFYIINIIVPCILTSVLAIFVFYLPPGAGEKMTLSISVLITLTVFMLLLADRVPETSLGIPIIVKYVMFTMILVTFSVILSVVVLNLHHRTPSTHIMPNWVRKVFIHILPRYIGMMRPNPEEPLLEKDSEKDDTSFRAFSGRQPGGEYLFRKINPDLVLPWRGRCESTVQLQQLPDSDRFCLILPPNLKSAIDAVTYIAEQLKKQDTDDSVRDWQFIALVVDRLFLWLFVIITTLGTLAVFLDASFNYTPDDPFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDH5</accession>
    <name>A0A3B5LDH5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000008615"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008721.1">
        <property type="protein sequence ID" value="ENSXCOP00000008615.1"/>
        <property type="gene ID" value="ENSXCOG00000006591.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155665"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="336" mass="37344" checksum="A54CEEE5688C53E7" modified="2018-12-05" version="1">MADKPSTLFVKLFAAGFYGFSSFLIVVVNKSVLTNYRFPSSICVGIGQMLATVVVLWVGKAARVITFPDLDESIPHKTFPLPLLYVGNQITGLFGTKRLLPMFTVLRRFSILFTMLAEGFLLKKFSRPVQLTVFTMILGAFIAAADLSFDLQGYVFILMNDVLTAANGAYVKQKLDAKELGKYGLLYYNALFMIVPTLLLAEVTGDMQKLQAAEFDGWSDAFFLSQFVLSCVMFILMYSTVLCTQYNSALTTTIVGCIKNVLVTYIGMVFSGDYIFSWTNFIGLNIIAGSLVYSYITLTEEQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LE11</accession>
    <name>A0A3B5LE11_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LE11"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007921"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008019.1">
        <property type="protein sequence ID" value="ENSXCOP00000007921.1"/>
        <property type="gene ID" value="ENSXCOG00000006081.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182856"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06893">
        <property type="entry name" value="PX_SNX19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003114">
        <property type="entry name" value="Phox_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037909">
        <property type="entry name" value="SNX19_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013937">
        <property type="entry name" value="Sorting_nexin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF50">
        <property type="entry name" value="SORTING NEXIN 19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08628">
        <property type="entry name" value="Nexin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02194">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00313">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51207">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PXA" evidence="4">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="392"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="553"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="795" mass="88395" checksum="916524E168465523" modified="2018-12-05" version="1">SFELKVHEKLFVFSQELTQKVLNLVGGHLQDYLTAKELAKEKYRSESEQLWKAYSSVATPHVAMSDDSAEVNYVRAIVALLLHILVPSPHLETNTGRFVVGELITSNVFLPLVAKLSDPDWLNLLIIEILCKSSTPLDTVASKLETCSLPLPAAESEAPTLQCVTQANSESLQQGAETELTDDTEAALPELHAYDATDTEEVKFPQTFSGEEEASRPFLRCYLRGRKSNPFYQETDSDPDSPLADYKPTSTDSLLMMGQEDTLYDRSRNYITTAEDNNSIDLEEVSPSPVDSSYPKVLLNSVLVDSPNDGCLSSLRDTEVTCSTDSLRDLKRENSSPGVSRSRELVLGVEPTGLGNPSELIMASPLQGCSPVSTFSFEPLSSPEGPVIIQNLRITGTITAKEHRGTGSHPYTLYTIKLMESIQIGSENPTPIQPVAYHMVNRRYSEFLNLQTRLEEKSELRKLIKGVKGPKKVFPEMPFGNMDSDKIEARKGLLETFLKQLCSISEIANSEEMQEFLALNTDARIAFVKKPFIVSRIDKIVVNAIVGTLKTAFPHSEPQSPTEDTESEVDGGKMGTDKKNRKGGICFLMPWFCCLLQVFNGMTLVDLQAFITEEEKSLQKETEMEGGPVLGQWVGCRGHGVRAEQCEEHDSAGGMSQAKVALNILCLLMKEQWSWLCSKNIQKTVRLLFGTLINRWLDVSVANLTCTKYWVVYLQVIQEAVWPGGTLPAAPPPHRSQQQKDSSKQQALDGLMKLLPDVVSDMLGSDKYRLSWQTALDSFQDPYINRQTYTTHPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LED0</accession>
    <name>A0A3B5LED0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sec1 family domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Syntaxin-binding protein 1-like 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. Involved in vesicular transport between the endoplasmic reticulum and the Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus</location>
            <location evidence="6">Golgi stack membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LED0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006219"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006293.1">
        <property type="protein sequence ID" value="ENSXCOP00000006219.1"/>
        <property type="gene ID" value="ENSXCOG00000004810.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074845"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.60:FF:000002">
        <property type="entry name" value="Sec1 family domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2060:FF:000002">
        <property type="entry name" value="sec1 family domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="617" mass="69832" checksum="474D38A942F001F9" modified="2018-12-05" version="1">MLNFNTPPLKNAAAEPVWKVLIYDRFGQDIISPLLSVKELRDMGITLHLLLHSDRDPIPDVPAIYFLMPTEENIDRICQDLRSQLYESYFLNFISAISRSKLEDIASAALAANAVCQVTKVFDQYLNFITLEDDMFILCHQNKELISYHAINRADIQDTDMEAIMDTIVDSLFCFFVTLGAVPIIRCPRGNAAEMVAVKLDKKLRENLRDARNSLFTGDSMTVGQFSFQRPLLVLADRNMDMATPLHHTWTYQALIHDVLDFHLNRVMMEEGAGTDPSQAGARPKKKTKKTYDLTAADTFWQKHKGSPFPEVAESVQEELDAYRAQEDEVKRLKSIMGLDGEDEGAISMLSDNTAKLTSAVSSLPELLEKKRLIDLHTNVATAVLDHIKSRKLDVYFEYEEKLMSKSTIDKSLLDVIGDPDAGTPEDKMRLFLIYYITAQQVPSETDLEQYRKALLNADCDLSPLNYIKQWKAFTKMASGPANYGNSGVKPMGLFSRVMNTGSQFVMEGVKNLVLKQHNLPVTRIVDNLMEMKSHPETDDYRYFDPKMLRGSESSIPRNKNPFQEAIVFVVGGGNYIEYQNLVDYAKSKPGRKVVYGCSELFNAAQFMKQLSQLGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEL1</accession>
    <name>A0A3B5LEL1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 13 member 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LEL1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009388.1">
        <property type="protein sequence ID" value="ENSXCOP00000009277.1"/>
        <property type="gene ID" value="ENSXCOG00000007068.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234550"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015139">
        <property type="term" value="F:alpha-ketoglutarate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015138">
        <property type="term" value="F:fumarate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017153">
        <property type="term" value="F:sodium:dicarboxylate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015141">
        <property type="term" value="F:succinate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071285">
        <property type="term" value="P:cellular response to lithium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF82">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="6" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="19"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="336"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="374"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="472"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="516" mass="57358" checksum="C4AF9CB71E1124F1" modified="2018-12-05" version="1">IIMGSMSDVFRWMWYHRNYIIIFITPLAFLPLPLIVPTSEAKCGYVIILMTLYWCTECMPLVVTALLPVILFPAMGIMKASDVSVEYLKDSSMLGVGGFLVAIAVENWNLHKRIALRVLLLVGVRPALLMTGFMIVSAFLSMWISNTATTAMMLPISQAVLHQLQATETRAEERDLQSAAEDNLGFEMESRKIKEGIPDEKNTDTKENALVTPESRRKAIEEKYNLLTKGMSLSVCYSASIGGTATLTGTAPNLILKGQIDKLFPGNGNVINFASWFGFAFPNMVLMLAISWLWLHFMFLGFNLKKSFGCGMKSDRDKEAYAVMKEEYKKLGGMKFAEGAVLTIFILLVLLWFTREPGFIPGWATVLFNKEDSFVSDGTVAIFMSMLFFVIPSQLPSCGSYGFTEAGETVTRVQNGKRIKAPPTLLNWQVVHERMPWSIILLLGGGFALATGKNGGPRVLDSLEYGSFLLQVKAGFILNIIGILCVNLGINTWGYIMFDMGTFPQWANNTHRSLIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEW7</accession>
    <name>A0A3B5LEW7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">YTH domain-containing family protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the YTHDF family. YTHDF2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LEW7"/>
    <dbReference type="SMR" id="A0A3B5LEW7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006404"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006484.1">
        <property type="protein sequence ID" value="ENSXCOP00000006404.1"/>
        <property type="gene ID" value="ENSXCOG00000004960.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156761"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990247">
        <property type="term" value="F:N6-methyladenosine-containing RNA reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098508">
        <property type="term" value="P:endothelial to hematopoietic transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008585">
        <property type="term" value="P:female gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071425">
        <property type="term" value="P:hematopoietic stem cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902036">
        <property type="term" value="P:regulation of hematopoietic stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.590.10:FF:000001">
        <property type="entry name" value="YTH domain family 1, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF8">
        <property type="entry name" value="YTH DOMAIN-CONTAINING FAMILY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="YTH" evidence="11">
        <location>
            <begin position="416"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="205"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="300"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="372"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="588" mass="62741" checksum="BE4DF48A301CBD93" modified="2018-12-05" version="1">MSASSLLEQVKRVQNGAVTQKDTLNDDEFEPYLNTQARQSNAYTAMSDSYMPSYYSPSIGFSYSLNEAAWSTGGDPPMPYLASYGQLSNGEPHYLPDAMFGQPGPLGSNPFLGQHGFNFFPSGIDFSAWGNSSSQGQSGTPQSSGYSSSYAYAPSSLGGAMIDGQSPFAPAANEPLNKAPGMNSLDQGMAGLKIGGAAPGGNGEMAPKVVGSGLPGGGPLGPVSSVGPPSMPPVSIAPAKPASWADIASKPAKPQPKLKTKGGMAGANLPPPPIKHNMDIGTWDNKGTMPKAATPHQVPSIPSNGQPPNQASPQPGATAAGNPQMPLSNGQLVPPGPPPPNHQQQQPSQPTRWVPPRNRANGFGDAGGSGSGQSPPSSSSVGVVPGVPSEPHPVLEKLRMVNNYNPKDFDWNLKQGRVFIIKSYSEDDIHRSIKYNIWCSTEHGNKRLDAAYRSLAGKGPLYLLFSVNGSGHFCGVAEMRSPVDYNTSAGVWSQDKWKGRFDVRWIFVKDVPNSQLRHIRLENNENKPVTNSRDTQEVPLDKARQVLKIIAGYKHTTSIFDDFSHYEKRQEEEECVKKSLNSFTGRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEX3</accession>
    <name>A0A3B5LEX3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 18">P2X purinoceptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">P2X purinoceptor 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">ATP-gated nonselective transmembrane cation channel permeable to potassium, sodium and with relatively high calcium permeability. Furthermore, CTP functions as a weak affinity agonist for P2RX1.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Receptor for ATP that acts as a ligand-gated ion channel.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Functional P2XRs are organized as homomeric and heteromeric trimers. Forms heterodimer with P2RX2. Forms heterodimer with P2RX4. Forms heterodimer with P2RX5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14 18">Belongs to the P2X receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LEX3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006409"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006489.1">
        <property type="protein sequence ID" value="ENSXCOP00000006409.1"/>
        <property type="gene ID" value="ENSXCOG00000004965.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230351"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004931">
        <property type="term" value="F:extracellularly ATP-gated monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001614">
        <property type="term" value="F:purinergic nucleotide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070588">
        <property type="term" value="P:calcium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033198">
        <property type="term" value="P:response to ATP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.490.10:FF:000001">
        <property type="entry name" value="P2X purinoceptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.940:FF:000006">
        <property type="entry name" value="p2X purinoceptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.940">
        <property type="entry name" value="atp-gated p2x4 ion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.490.10">
        <property type="entry name" value="atp-gated p2x4 ion channel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003044">
        <property type="entry name" value="P2X1_purnocptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027309">
        <property type="entry name" value="P2X_extracellular_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001429">
        <property type="entry name" value="P2X_purnocptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059116">
        <property type="entry name" value="P2X_receptor"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00863">
        <property type="entry name" value="P2X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10125">
        <property type="entry name" value="P2X PURINOCEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10125:SF9">
        <property type="entry name" value="P2X PURINOCEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00864">
        <property type="entry name" value="P2X_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005713">
        <property type="entry name" value="P2X_purinoceptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01308">
        <property type="entry name" value="P2X1RECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01307">
        <property type="entry name" value="P2XRECEPTOR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 14 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 14" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="338"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="70"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="294"/>
            <end position="296"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between two neighboring subunits of the homotrimer</note>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="17">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="118"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR005713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR005713-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005713-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR005713-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="387" mass="43134" checksum="24469E770AD588C1" modified="2018-12-05" version="1">MTMKNQITSALSDFFFEYETPRQVLVRNRRVGVVCRLIQLGVLVYIIGWVFIYEKGYQSTDTAVSSVLTKMKGVGYTNVSGIETVWDVADYVFPPQGDSSFVVMTNYIITEGQKMGKCPELKGMHDCTSNADCVGGSFKRTGHGQMTGVCVNKTQTCEVLAWCPIEDDHTIPNPPLLISAENYTLFIKNSVTFPLFGVTRSNLVEGIDGNYIGKCLFHPKKEPLCPIFKLGDIVKLSGFSFEKLAQEGGAIGIVVDWTCNFDVDVKHCKPEYNFHGLYGNPSETDSASASVGYNFRYAKHYMEDKIPKRTLLKVFGIRFDVIVKSLARKFDIIPTLTAIGSGVGIFGVATVVCDLVLLYLLPKREFYKNMKFKYTDTQTQVSSVSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFV3</accession>
    <name>A0A3B5LFV3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFV3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008667.1">
        <property type="protein sequence ID" value="ENSXCOP00000008561.1"/>
        <property type="gene ID" value="ENSXCOG00000006551.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177209"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="133"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="17"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="259" mass="30214" checksum="AA5982F6FDB7F04B" modified="2018-12-05" version="1">MVVLIIIIEWNTKQNVITSIEKVKEEMKSNTEKLLEEKSRISEQLNLTNTNFSILVAEYERLLGRIPCNLTLLEKVNQLTSQLKSTQNKYQLTELENKKQRSILSSKRLDYIWNRCDRTTLQCSRCLPGWTEHASRCFLLSNVEKTWEDARVDCLNYKGDLAVVLNAADQAFLTNLTFQFKKANPGMNFHSAWIGLQDMVKEGVHFWVNGERITWDVKYWRPGEPNNAKLGYTLGPVWFTQYADDCSIYYSNFFFTPLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGJ9</accession>
    <name>A0A3B5LGJ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fizzy-related protein homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cdh1/Hct1 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGJ9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009982"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010100.1">
        <property type="protein sequence ID" value="ENSXCOP00000009982.1"/>
        <property type="gene ID" value="ENSXCOG00000007573.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183104"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000025">
        <property type="entry name" value="FIZZY-related 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF35">
        <property type="entry name" value="FIZZY-RELATED PROTEIN HOMOLOG ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="11">
        <location>
            <begin position="170"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="216"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="299"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="429"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="483" mass="53381" checksum="D4C27AE2D8BE3AA1" modified="2018-12-05" version="1">MDQEYERRLLRQINHQNLHTERRMTKVSRTAAVKSGDRFIPTRAGSNWSINFHYANENCHSPAQNHKAKDAGSDSSKDAVAYAALLRNELLGAGIETVAEPHADDRRHEVLAPDSCSLFRYAVHTKRVPCDGGNEVSPFSLSPLSNKSHKLLRSPRKPARKISKIPFKVLDAPELQDDFYLNLVDWSAGNLLSVGLGACVYLWSACTSQVTRLCDLSVDGDSVTSVCWNERGSLVAVGTHKGYVQIWDAAGGRKLTCLEGHSARVGALAWNGEQLSSGSRDRVILQRDVRTPPATERRLQGHRQEVCGLKWSPDHQHLASGGNDNKLLVWNSSSLVPVQQYSDHLAAVKAIAWSPHQHGLLASGGGTADRCLRFWNTLTGQALQSTDTGSQVCNLAWSKHANELVSTHGYSQNQILVWKYPSLTQVAKLTGHSYRVLYLAVSPDGEAIVTGAGDETLRFWNLFSKTRCTKESKSVLNLFTRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGY8</accession>
    <name>A0A3B5LGY8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RAN binding protein 10</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RANBP9/10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGY8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010122"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010241.1">
        <property type="protein sequence ID" value="ENSXCOP00000010122.1"/>
        <property type="gene ID" value="ENSXCOG00000007678.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158257"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd12909">
        <property type="entry name" value="SPRY_RanBP9_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000011">
        <property type="entry name" value="RAN binding protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035782">
        <property type="entry name" value="SPRY_RanBP9/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="3">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="CTLH" evidence="4">
        <location>
            <begin position="247"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="311"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="311"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="361"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="397"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="579" mass="62677" checksum="586B517302E782DF" modified="2018-12-05" version="1">PHPGGLDAILTGVPIFSLQGHANSLPRLTVHKHSCSVESHGKNHKDAASVRATHPIPAACGIYYFEVKIVSKGRDGYMGIGLSAQGVNMNRLPGWDKHSYGYHGDDGHSFCSSGTGQPYGPTFTTGDVIGCCVNLINNTCFYTKNGISLGLSLPNLYPTVGLQTPGEIVDANFGQQPFVFDIEDYMSEWRAKIHGMITSFPIGERLGEWQAVLQNMVSSYLVHHGYCATATAFARATETTIQEDQTSIKNRQRIQKLVLAGRVGEAIDATQQLYPGLLERNPNLLFMLKCRQFVEMVNGTDSEVRCLTVRSPKSQDSYPGSPNLSPRHAASGTHVHSGGAESPTCSNGVAAPNKAKVHGGSSIKYPSASSSTSSSSSSSPSSINYSESNSSDSTKSQPHSANSNQETSDSEMEIEAEHYTNGVVDGSSARIMNGTYQHEEILQPDDNSIYEGRRSSKQLCGGNQAATERMIQFGRELQALSEQLCHDYGTNSTHKKMLQDAFSLLAYSDPWNCPVGQQLDPTQRESLCSALNSAILESQNLPKQPPLMLALGQATECVQLMARVRSGSCSFARIDNFLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHS6</accession>
    <name>A0A3B5LHS6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lateral signaling target protein 2 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Zinc finger FYVE domain-containing protein 28</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Negative regulator of epidermal growth factor receptor (EGFR) signaling. Acts by promoting EGFR degradation in endosomes when not monoubiquitinated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lst-2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHS6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011683"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011816.1">
        <property type="protein sequence ID" value="ENSXCOP00000011683.1"/>
        <property type="gene ID" value="ENSXCOG00000008820.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157217"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15731">
        <property type="entry name" value="FYVE_LST2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000092">
        <property type="entry name" value="Lateral signaling target protein 2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043269">
        <property type="entry name" value="FYVE_LST2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051118">
        <property type="entry name" value="LST-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46465">
        <property type="entry name" value="LATERAL SIGNALING TARGET PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46465:SF2">
        <property type="entry name" value="LATERAL SIGNALING TARGET PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="17">
        <location>
            <begin position="842"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="293"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="394"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="459"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="699"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="300"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="340"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="395"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="482"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="912" mass="100373" checksum="5628AFDCA820623D" modified="2018-12-05" version="1">SLNRKRNWRTDPQLLAQFYYADEELNQVATELDSLDGRKDPQRCTLLVNQFRSCQDNVLNIISQIMDECIPNDRANRDFCVKFPEEIRHDNLAGQLWFGAECLAAGSIIMNREIESIAMRPLAKDLTRSLEEVRSITRDQALRDLNYYTDRMKDALRHFDSLFAEFELSYVSAMVPVKSPKEYDVQQEVIVLFCETVERALRLGYLTQDMIDDYEPALMFTIPRLAIVCGLVVYPEGPLNLDRKSEDMSELFRPFRTLLKKIRDLLKTLTEEELLSLEKNLCISQDGELASNGIPVPVQENPSSHSLANDASNGQGSGEEDPLPVFVSPDQDKELAEVESGWEEVETQNGEEEPEQGLLCEEAEEAELACSMQYDEEELEQLNMMVYRVGDEMSTLLSPPSQGQSPARRPSRGEPAGSSGASSTQASPRRMAANRIRTGVYVEEEDRVFFMEDVDAAGDGSSLPKGSTPPLQQKPGPGSGINGWSSATTSEQPCPTAKRPLCSHAPRNEPLPYSNGWDRGLEGTASETAEVIAHRMGGMKLSATVIFNPRSPSLTELAVDKLLLPRPAASEIEPCGPLVATHCLLNSCVCCGSCEDAHEDGGVASDTAGLELGLALGLDKHCKRPASSAVIQSAACQLPPRAHQRFAKGELAQQTPPSALVSFFISVCFILVVFSFQKSPLSSVSGSDCDSVSVTTCSLSSSAYTPSPVSSLTPSSGTSEDLDQQEIRLALQDARVAARSKIRSRFHSSSDLIHRLFVCISGVADQLQTNYASDLRSILKTLFEVMATKCEQGDNNKQKKGPVLRSAVLEDCALCQETISSSELAAKAREGQFEDPPDWVPDDACNSCITCKAPFTVIRRKHHCRSCGKIFCSRCSSHSAPLPRYGQVKPVRVCTHCYMFHVTPFYSDKPAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHV2</accession>
    <name>A0A3B5LHV2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">BBSome complex member BBS1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="16">Part of BBSome complex, that contains BBS1, BBS2, BBS4, BBS5, BBS7, BBS8/TTC8, BBS9 and BBIP10. Interacts with the C-terminus of RAB3IP. Interacts with CCDC28B and ALDOB. Interacts with PKD1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
            <location evidence="2">Centriolar satellite</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHV2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010437"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010560.1">
        <property type="protein sequence ID" value="ENSXCOP00000010437.1"/>
        <property type="gene ID" value="ENSXCOG00000007907.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005232"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034464">
        <property type="term" value="C:BBSome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005113">
        <property type="term" value="F:patched binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005119">
        <property type="term" value="F:smoothened binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061512">
        <property type="term" value="P:protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001895">
        <property type="term" value="P:retina homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007608">
        <property type="term" value="P:sensory perception of smell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002248">
        <property type="entry name" value="Bardet-Biedl syndrome 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028784">
        <property type="entry name" value="BBS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032728">
        <property type="entry name" value="BBS1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056419">
        <property type="entry name" value="GAE_BBS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20870">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20870:SF0">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14779">
        <property type="entry name" value="BBS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23304">
        <property type="entry name" value="GAE_BBS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="15" id="KW-0844">Vision</keyword>
    <feature type="domain" description="Bardet-Biedl syndrome 1 N-terminal" evidence="18">
        <location>
            <begin position="40"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Bardet-Biedl syndrome 1 protein GAE" evidence="19">
        <location>
            <begin position="503"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF14779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF23304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="610" mass="67367" checksum="C7DB73AC7C3628CF" modified="2018-12-05" version="1">MTLIYFLYSCRSHLLSSANSLISCPVVMSSVDSSGDGGKWLDAHYDPVAGLYTFSSCIDLADLSGDRESRLVVGDLGTGSSGMKLKVYRGTALISENSLLDLPAGLVAFFMDLHEPRIPAVAVASGPCIYVYKNLRPYFKFTLPSLDINPLEQDVWQQAREGQIDPVMLKEMLESIRKKADVPLSVRSLRFLSLESDQVDEFVQLYKQQPIRRQTVITCIGTLKKSTADEDGISCLVIGTENSNVFILDPEAFTILCKMSLPAVPTMMDVTGQFDVEFRITVACRNGSIYILRRESEKPKYCIELSSHPVGLVRVGKNMVVGCTDESLQGFTQKGKKLWKIILPAPVTTMAAMDLPTRGLQAVLVGLANCQVHLYRDKNLLSTIKTPDTVTSICFGRYGREDGTLIMTTKGGGIMVKILKRTAVFDAMDSTPGPPLAQSVRLNVPKKTKLYVDQTLRERENGLAMHRAFHMDLSRTRVAAARAYVKALESSLTPMSSSLSKPLKMNAVVQGLGPSFKLTLNIQNTASCRPIMNLAINFLYDNNLYRIRNPYRKIPLLVPGLIYPVETFVECTSDKGISDIIKVFVLHEEKSSPLLTAHINMPVSEGLVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LI49</accession>
    <name>A0A3B5LI49_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane protein 53</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus outer membrane</location>
            <topology evidence="6">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMEM53 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027876639.1">
        <property type="nucleotide sequence ID" value="XM_028020838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LI49"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010290"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010413.1">
        <property type="protein sequence ID" value="ENSXCOP00000010290.1"/>
        <property type="gene ID" value="ENSXCOG00000007800.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114146602"/>
    <dbReference type="KEGG" id="xco:114146602"/>
    <dbReference type="CTD" id="79639"/>
    <dbReference type="GeneTree" id="ENSGT00390000000715"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008547">
        <property type="entry name" value="DUF829_TMEM53"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12265">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12265:SF30">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05705">
        <property type="entry name" value="DUF829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="32312" checksum="BA78AE0C65FF81C3" modified="2018-12-05" version="1">MAADDIDYNIVFPDAGTSERHWQGTKEPVVILLGWAGCKDKHLSKYSSIYNDKGCVTIRYTAPLKTVFISETFGYKELSGTALKLLEVLYDYEVENSPVFFHVFSNGGFMLYRYIVELLHSDKQFSSLRVLGAVVDSAPGSGNVRGALRALTATLGPRISPVLKYVLLALFAVSVFLLRIVLYPVTKFIHKNHYDAVRERPPAWPHFLLYSGADQVIRHRDIELFAETVVKKGVAVHSCDFASSAHVCHFRDFPEQYSRKCRDFLVACMKDSKGPETKKRQHVQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIB1</accession>
    <name>A0A3B5LIB1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIB1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010731.1">
        <property type="protein sequence ID" value="ENSXCOP00000010607.1"/>
        <property type="gene ID" value="ENSXCOG00000008022.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017222812" description="Secreted protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="97" mass="10361" checksum="8214E9D10558BDA6" modified="2018-12-05" version="1">FCVLFCVCVGAPFFPACVCQLCTMVSIACMCVCVCKCSFTYVFSCCAPKMAEASGTISSALYALLTQGTTYKLLTLPYCLLSLLSISLSASDTHLYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJP5</accession>
    <name>A0A3B5LJP5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Neurexin 3b</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Neuronal cell surface protein that may be involved in cell recognition and cell adhesion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJP5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011122"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011249.1">
        <property type="protein sequence ID" value="ENSXCOP00000011122.1"/>
        <property type="gene ID" value="ENSXCOG00000008405.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167343"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000015">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000029">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000004">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000005">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000007">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF48">
        <property type="entry name" value="NEUREXIN-3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 10" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Laminin G" evidence="11">
        <location>
            <begin position="45"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="241"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="11">
        <location>
            <begin position="283"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="11">
        <location>
            <begin position="469"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="647"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="740" mass="82210" checksum="AA2E0602AC92CC59" modified="2018-12-05" version="1">AATVTVVYKNNDIRLELSRLARIVDPKMKLQGDVVFKCENVPTLDPINFETPDSFLALPKWNTKRVGSISFDFRTSEPNGLILFTHGKPQNRRDAKGQKNNKVDFFAVELLDGGLYLLLDMGSGTIKVKATQAKVSDGAWHHVDIQRDGRSGIISVNSRRTPFTASGENEILDLEGDLYLGGLPDSRAGLVLPTELWTAMLDYGYVGCIRDLFIDGRSKDIRQISQSQNATGIKSSCSKMTGKQCDSSPCKNSGMCKEGWNRFICDCTGTGFWDSTCEREASILSYDGSMYMKVVIPNIMHTEAEDVSLRFMSQRAFGLLMAATSRESADTLRLELDSGRVKLTVNLDCVRINCNTSKGPEVLYAGQKLNDNEWHSVRVVRRGKNFKLIVDDDMAEGQMAGDHTRLEFSNVETGILTEKRFVSSTPSPFIGHLQSLKFNGMQYIDMCKNGDIDFCELNARFGMRFIVADPVTFKTKGSYLGLATLQAYTTMHLFFQFKTTSPDGFILFNSGDGNDFIAVELVKGFIHYVFDLGNGPSLLKGNSDNPLNDNQWHNVVITRDASNTHTLKVDTKSVTQNVNGAKNLDLKGDLFVGGLGPNMYQNLPKLVVSREGFQGCLASVDLNGRLPDLISDALFRSGHVERGCEGPSTTCQDDSCANMGVCIQQWENFTCDCSMTSYTGTHCNDREWTQMLAFFLDDSFTKKTNCLLKQQMQLQGQGFERKINNGCLQFLGESERGSVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LK44</accession>
    <name>A0A3B5LK44_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ADP-ribosylation factor-like protein 15</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LK44"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012448"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012599.1">
        <property type="protein sequence ID" value="ENSXCOP00000012448.1"/>
        <property type="gene ID" value="ENSXCOG00000009409.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156244"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000934">
        <property type="entry name" value="ADP-ribosylation factor-like 15 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042292">
        <property type="entry name" value="ARL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46693">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46693:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017281736" description="ADP-ribosylation factor-like protein 15" evidence="7">
        <location>
            <begin position="20"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="137"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00072404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="199" mass="22051" checksum="0852574098B8B49B" modified="2018-12-05" version="1">PSDMRVFLILPCFRALCCKGPPPPRPEYDVVCIGLTGAGKTSLLSRLCSESTDNIVPTTGFSIKAVPFPNAILNVKELGGADNIKKYWSRYYQGSQGVIFVLDSASSDEDLEAARNELHSALQHPQLCTLPFLILANHQDKPAARAPNQIKKYFELEPLARGKRWILEGCTVDNMESMKESFGQLISLLEDKETEPARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKE3</accession>
    <name>A0A3B5LKE3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="1">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">USP1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKE3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008359"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008465.1">
        <property type="protein sequence ID" value="ENSXCOP00000008359.1"/>
        <property type="gene ID" value="ENSXCOG00000006410.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00910000144243"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02671">
        <property type="entry name" value="Peptidase_C19O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033815">
        <property type="entry name" value="USP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF905">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="3">
        <location>
            <begin position="91"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="228"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="349"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="606"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="671"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="228"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="246"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="258"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="283"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="349"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="359"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="606"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="779" mass="86897" checksum="2674E5F534801629" modified="2018-12-05" version="1">MNRVHTHAHREVRTHRLTHTHQGLRGQGVSQSSKPLRRHSLKKEQVCIFLFLCGYVSLCTCDQVVYGPAPCPSSPGLPIPCEKKENLVPFVGLNNLGNTCYLNSILQVLYYCPGFREGVKNLYDLSKRPEKPNDETVKNEETVPAHIELLGSFSSLMTSVEQLQSSFLLNPNGFSEGELATPPRKILHTIRQLNPMYEGYLQHDAQEVLQCILGYIQEACDTIRKEQELERKEDDDRDVKKENGIPSDSSFSGTESRASTDEVSGKRKSDTEVGNAKKKPKSKKSESTEENVCRNKPFTRSKRKSSSDITVDSTQSKEGEQQKKRGTLGWLRSSGKQPSIFSKFCSVGKISSTNPKPQNKSEPENTPSEEPKQKSTQQSVARKFNFCLESSMFVRALIMIQVSLFASDVLGLMEKLFQGRLVLRTRCLECESFTERREDFQDISVPVLDDQPKSPEDLFEVSPDPKPEMKTLTWAISQFASVERIVGEDKYFCETCHHYTEAERSLLFDKTPEVITIHLKRFSASSLEVDPYAGLSKVNTPLQTPLTLSLNEWCTKPSAATGQQYELFAVVMHSGVTISSGHYTTYIRMSDLKDAKVWLQAKKEKVTEEDVKESKEGAQTDENTTFDDGEVSVSLSTRGQRRAGLASTKSGSKKLLEGGVGLLGGQRSLPNCDLGSGSKAANRDAAEGSKRRKTLSSSSQNAGGLKKEPEDAPMAPPGGMEAAEQQALTSLLEYEGKWLLFDDSEVRLFVEEDFLRACSPETCSSSTPYLLFYKRMPEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKJ9</accession>
    <name>A0A3B5LKJ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Neuronal vesicle trafficking associated 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NSG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027887972.1">
        <property type="nucleotide sequence ID" value="XM_028032171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKJ9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008525.1">
        <property type="protein sequence ID" value="ENSXCOP00000008419.1"/>
        <property type="gene ID" value="ENSXCOG00000006457.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114153554"/>
    <dbReference type="KEGG" id="xco:114153554"/>
    <dbReference type="CTD" id="51617"/>
    <dbReference type="GeneTree" id="ENSGT00390000000483"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009431">
        <property type="entry name" value="NSG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28546:SF2">
        <property type="entry name" value="NEURONAL VESICLE TRAFFICKING-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28546">
        <property type="entry name" value="NEURONAL VESICLE TRAFFICKING-ASSOCIATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06387">
        <property type="entry name" value="Calcyon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002383">
        <property type="entry name" value="Calcyon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="19816" checksum="2197AC3D3275A8C3" modified="2018-12-05" version="1">MVKLGSNLQDKGAKAVSVEDGFQNVPLITPLDVGSLQSQAPDKVVVKTRTEYQTEKKRLKVPKVEEFTISFTDGVSERLKVTILIILALAFLACIVFLVVYKAFTYDHACPDGFIYKHKRCIPASLEAYYAAQDANSRGRFYTVISHYSMAKQTTSHSVSPWLPGGAAGHEAKPPRGEGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LN95</accession>
    <name>A0A3B5LN95_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000009329"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009440.1">
        <property type="protein sequence ID" value="ENSXCOP00000009329.1"/>
        <property type="gene ID" value="ENSXCOG00000007100.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155936"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
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        <property type="term" value="P:endocytosis"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="EH"/>
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        <property type="entry name" value="SH3_Intersectin_3"/>
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        <property type="entry name" value="Intersectin-1 isoform 1"/>
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        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Dbl homology (DH) domain"/>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="5"/>
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        <property type="entry name" value="C2_domain_sf"/>
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        <property type="entry name" value="DBL_dom_sf"/>
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    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
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    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
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    <dbReference type="InterPro" id="IPR002048">
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    </dbReference>
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        <property type="entry name" value="EH_dom"/>
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    <dbReference type="InterPro" id="IPR051480">
        <property type="entry name" value="Endocytic_GEF_Adapter"/>
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    <dbReference type="InterPro" id="IPR001331">
        <property type="entry name" value="GDS_CDC24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
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    <dbReference type="PANTHER" id="PTHR46006:SF6">
        <property type="entry name" value="INTERSECTIN-2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46006">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR AT 64C, ISOFORM A"/>
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    <dbReference type="Pfam" id="PF00168">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="EH"/>
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        <property type="entry name" value="PH_13"/>
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    <dbReference type="Pfam" id="PF00621">
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        <property type="entry name" value="P67PHOX"/>
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        <property type="entry name" value="SH3DOMAIN"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="EFh"/>
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        <property type="entry name" value="RhoGEF"/>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
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    </dbReference>
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        <property type="entry name" value="PH domain-like"/>
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        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
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        <location>
            <begin position="78"/>
            <end position="166"/>
        </location>
    </feature>
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        <location>
            <begin position="110"/>
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        </location>
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            <begin position="307"/>
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        <location>
            <begin position="340"/>
            <end position="375"/>
        </location>
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        <location>
            <begin position="795"/>
            <end position="856"/>
        </location>
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    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="875"/>
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        </location>
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        <location>
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            <end position="1015"/>
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        <location>
            <begin position="1084"/>
            <end position="1143"/>
        </location>
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        <location>
            <begin position="1166"/>
            <end position="1352"/>
        </location>
    </feature>
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        <location>
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            <end position="1500"/>
        </location>
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        <location>
            <begin position="1508"/>
            <end position="1624"/>
        </location>
    </feature>
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            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
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            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
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            <end position="513"/>
        </location>
    </feature>
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            <end position="793"/>
        </location>
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        <location>
            <begin position="1335"/>
            <end position="1362"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="700"/>
            <end position="792"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    <evidence type="ECO:0000256" key="12">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        </source>
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        </source>
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    <evidence type="ECO:0000259" key="16">
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    </evidence>
    <evidence type="ECO:0000259" key="17">
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    <evidence type="ECO:0000259" key="18">
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
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    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009329.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1651" mass="188037" checksum="B12EE650247DCB90" modified="2018-12-05" version="1">MWPIIEHRTSIGLQQHKCQSVVASFEALHCPSYSQTIRPALIHAAWRLTGCGCGHASCMSQWPCAECGSNVWAITPEERGKHDKQFDTLAPILGYISGEQARKFFLQSGLPASVLAEIWNLADMDSDGKMDRLEFSIAMKLIKLTLQGRNLPSSLPVTMKQPPTLNSSSNMSSSARFGMGSMPNLSVGLSSMPTISTMPVLTPMLANRPLPSVQPLVPVPMTLPLITSFGSSGLPNGTVSLLTSPLVPSTPMSGFSSPMAFSPSPGVSKANSLLDLGSSSSNSSSTTSLASNSPKMGSGDWAVPQASRLKYRQQFNTLDKLMSGYLSGPQVRNALMASNLTQTQLATIWSLADVDKDGQLRAEEFILAMHLVDMAKTGHPLPLTLPQDLIPPSLRGGIKSSELLNGTGPYISPSLIDTTEIEPPQKNKSSVSYEDKLKENFARGSAELEKRRLALVEEQRKERERREREEKEAQDRREREARELENRRKLEEERRMERQREMERQREEERLRELERKEAAKQEMERQRREEWERAKLEELGRRKETEQEEIIRLRARKKSLELELEAVGNKHKQISDRLRDAQSKRRIQKAEVDLINQKRDARIAEINTLQLEFEEWQRKLSQLVPEQQQLSEKLRNISLKKLPSATLTSLMGSAAESSGNCRRLKDQLDILEKETTDKLTQMEQYNKELKVSGQLAKLEEEKREQERREQERREREEEEARQRRLQEEQRARQREEEEREAQARLRAAQEKAQEEERRRRDEEERKKRELEEEMKQKEMEEEKRKKQEQERGAAQLTSYRALYSFLGRNADELSIDADCLIEVDEHTVGEPGWLCGNYRGKRGWFPQSYAEKCPTPPTSEMALPSSSLPPLNSTVLLAARAVSSWSPASETHLNLSPDGDLISVLQQRDDWWLGQFNGTRGWFPKSYVTPEPGGNEVSFIHHPFCTKTKKIIAVKLSELEYVALYTYESPETDDLTFDEGDVIMVTEREGEWWRGCIGDQTGLFPSNYVRPVEPEAVTATEALMAYQLTLSPGQLIVVRAKNSTGWWLGELQARGKKRQKGWFHSSHVKLLGPSSNKTCLSPLPVCQVIAMYDYAAANQDELSFSKGQLINILDKTNPDWWKGEINGVMGLLPTNYVKMTTESDPSQQWCADLITLDTMTPQERKRQGYIHELFHTEETYVDDLELVLEVFHKPMSESGRLTEAEMATIFVNWRELIMCNTRLLNALRERKQTGGENMPVQLIGDLLASELAHMQPYVCFCSSQLNAAALLQTKTHNQPEFKNFLKKVATNYRCKGMPLSSFLLKPMQRLTRYPLLIKNILEHTPDGHEDREPLREALERAEELCSQVNEAVREKENADRLEWIQNHIQCEGPIEHLVFSSLTNCLGPRKLLHSGRLFKTKSSRELWAFLFNDFLLLTHSAKPFSSSGSEKLFSPKTNMQLKIYKTPLFLNEVLVKMPADPSSDEPLFHVSHIDRVYTLKTETLSERTAWVQKIKAASEHFIETEKKKREKAYQARSLKSSGIGRLLVTVTEAQELKACKPNGKSNPYCELTMGAQCYTSRPVLDTLNPKWNFNCQFFIKDIYQDVLSITVFEKDQFSPDDFLGRAEVPVATIKKDMDSKGAATRRLLLHEVPTGEVSVKLDLQLYEATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LS08</accession>
    <name>A0A3B5LS08_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LS08"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014027.1">
        <property type="protein sequence ID" value="ENSXCOP00000013857.1"/>
        <property type="gene ID" value="ENSXCOG00000010490.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="80"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="153" mass="17267" checksum="380015608587AF84" modified="2018-12-05" version="1">MTKDVLRGAKKNRNTEQEAGTTKLIEGLKAEMLELKKLVLETVEITKKHQTERATTHSYRKPRGCPACLEAQSGERCTHCYKCGQGGHLARGCRQNRGQQGNWTGLLGRDPHQKSKLLIYQSKFLPSSMIMSFGSCPKEQDQGYKRTKLVLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUA9</accession>
    <name>A0A3B5LUA9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Family with sequence similarity 135 member A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUA9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015781.1">
        <property type="protein sequence ID" value="ENSXCOP00000015588.1"/>
        <property type="gene ID" value="ENSXCOG00000011787.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157565"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="14798" checksum="8AD3D24B2A859F2E" modified="2018-12-05" version="1">MTEVQVTVEFSVELHKFYNVDLFQRGFYQMRGSLKVPPRVPHKVETSLLHPGGSDLAFPASVQDDVICSKTFQILYKNEEIVVNDVLLFKVMMLLDEKKVEESLNEMDFQLCLDLYFTDGDYTYVSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWH6</accession>
    <name>A0A3B5LWH6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027900210.1">
        <property type="nucleotide sequence ID" value="XM_028044409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWH6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015235"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015426.1">
        <property type="protein sequence ID" value="ENSXCOP00000015235.1"/>
        <property type="gene ID" value="ENSXCOG00000011544.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114161223"/>
    <dbReference type="KEGG" id="xco:114161223"/>
    <dbReference type="CTD" id="503566"/>
    <dbReference type="GeneTree" id="ENSGT00940000160974"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001591">
        <property type="term" value="F:dopamine neurotransmitter receptor activity, coupled via Gi/Go"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007195">
        <property type="term" value="P:adenylate cyclase-inhibiting dopamine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071875">
        <property type="term" value="P:adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051481">
        <property type="term" value="P:negative regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051967">
        <property type="term" value="P:negative regulation of synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060158">
        <property type="term" value="P:phospholipase C-activating dopamine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014059">
        <property type="term" value="P:regulation of dopamine secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043266">
        <property type="term" value="P:regulation of potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000066">
        <property type="entry name" value="Dopamine receptor D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002185">
        <property type="entry name" value="Dopamine_D4_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000929">
        <property type="entry name" value="Dopamine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF145">
        <property type="entry name" value="DOPAMINE RECEPTOR D4 RELATED SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00569">
        <property type="entry name" value="DOPAMINED4R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00242">
        <property type="entry name" value="DOPAMINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="367"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="36"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="284"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="298"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="406" mass="44996" checksum="C119A81CB915B7BF" modified="2018-12-05" version="1">MENVTLGSPAGEARMRDYNYLALVLGVLLILIIILGNVLVCLSVLTERSLKTATNYFIISLAVADLLLAVLVLPLYVYSEFLGGTWTLSTSICDALMTMDVMLCTASILNLCAISVDRYIAVVVPLKYNRNQFSVRQLTLITATWVLSLGVASPVIFGLNQVPGRDPTVCKLEDDYFVVYSSVCSFFVPCPVMLFLYYWMFRGLRRWSSRSRSQAHRVGRRGLSSHLALALRRAKDTPGSQEKVVYTVPPISPTSPSIFSMATPCATLLTEENQHGAVMAADSDPVTTQMDSVSDGEPTGRWEGSSRRENATKRGRRTSKTSRVSGRERKAMKVLPVVVGVFLACWTPFFTVHVTKVLCRSCNIGPTLISVVTWLGYVNSAVNPIIYTIFNAEFRNVFHRLLCCRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWN8</accession>
    <name>A0A3B5LWN8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Si:dkey-148a17.6</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWN8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016358"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016564.1">
        <property type="protein sequence ID" value="ENSXCOP00000016358.1"/>
        <property type="gene ID" value="ENSXCOG00000012357.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182966"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004974">
        <property type="term" value="F:leukotriene receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14975">
        <property type="entry name" value="7tmA_LTB4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000109">
        <property type="entry name" value="Leukotriene B4 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF946">
        <property type="entry name" value="LEUKOTRIENE B4 RECEPTOR 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="74"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="212"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="27"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="339" mass="37578" checksum="F5A0701D3C7F5E71" modified="2018-12-05" version="1">MALEEFDGGTVVACVILGLSFLVGAPGNLLVIWTILKHVKKRSHTVVLILHLAAADLVVLITLPLWIYSLAQSWVFGVTCCKAMVFVINACMYSSVFLITLMSVERFVAVRYPFVSVVWKKKQALNKVLLALWSAGFLFSIPIIPTQTIGVDDGKTHCLYREYTSDTQELVCLLLETVVGYMLPFSILVVCYGCLCSRITQMNFRSKRKSTVLIASVVVAFAICWTPHHIGNIISIVILAIDDSKSNIALNLEAARQTMAFVAGAMVFISSSINPVLYMFAARSFRESLRDTGIQKLFRHISSTSPGEGNREISFVSRRQSNQTNCSQLMTESKDDTNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXP5</accession>
    <name>A0A3B5LXP5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Torsin family 2 member A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ClpA/ClpB family. Torsin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027890019.1">
        <property type="nucleotide sequence ID" value="XM_028034218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXP5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015680"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015874.1">
        <property type="protein sequence ID" value="ENSXCOP00000015680.1"/>
        <property type="gene ID" value="ENSXCOG00000011846.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114154801"/>
    <dbReference type="CTD" id="27433"/>
    <dbReference type="GeneTree" id="ENSGT00950000182888"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002276">
        <property type="entry name" value="Torsin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049337">
        <property type="entry name" value="TOR1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010448">
        <property type="entry name" value="Torsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10760">
        <property type="entry name" value="TORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10760:SF4">
        <property type="entry name" value="TORSIN-2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21376">
        <property type="entry name" value="TOR1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06309">
        <property type="entry name" value="Torsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017453586" description="Torsin family 2 member A" evidence="9">
        <location>
            <begin position="20"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Torsin-1A C-terminal" evidence="10">
        <location>
            <begin position="259"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="314" mass="35212" checksum="19F60E41C93004BD" modified="2018-12-05" version="1">MLAVLPALLLLLLLSSRSACGVFQKLYCSFSDSCDCDFKPNIRDLEWDLYKNVFGQHLAQDLVAEEVSRFLLNKSPDRPLVLSFHGSSGTGKTLVSTMLGNHLYGSAMSSPFVHQFVPALHFPSAKRVAQYQAELKNWVKGNLTACSRSVFIFDEMEKMPAGLIDVLEPFLGPSHVVFRTNYRKAIYVFISTAGQEAIHRLVLENRRAGRDRDEIKPSDLQEVVQQSVYSNKTSGLYLSSIVQQNLITRFVPFLPLTRTHVERCVRAQLCQRGSCNRDDVVEAVGRDMTYDPAEGHYFSTTGCKAVAAKISFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYA3</accession>
    <name>A0A3B5LYA3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">UPAR/Ly6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYA3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016342.1">
        <property type="protein sequence ID" value="ENSXCOP00000016137.1"/>
        <property type="gene ID" value="ENSXCOG00000012195.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163304"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050918">
        <property type="entry name" value="CNF-like_PLA2_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016054">
        <property type="entry name" value="LY6_UPA_recep-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035076">
        <property type="entry name" value="Toxin/TOLIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20914:SF9">
        <property type="entry name" value="COILED, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20914">
        <property type="entry name" value="LY6/PLAUR DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00087">
        <property type="entry name" value="Toxin_TOLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00021">
        <property type="entry name" value="UPAR_LY6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="UPAR/Ly6" evidence="8">
        <location>
            <begin position="4"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Snake toxin/toxin-like" evidence="9">
        <location>
            <begin position="80"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="19089" checksum="7B927747EA222268" modified="2018-12-05" version="1">CLSSSPGICTGKTTECPSQDYRYGVVVPIVNFKGCMMPELCVENSVNYGTYKVVKNSVCCNGDLCNAQIPEYKSAPNGKKCFTCEGENCMKTLKCEGNENYCVKATRAQYEGKNVTLKGCASKLICSDQRASRVNQFSTEQLSCCQGDYCNSASRAFTYLLLLLVPLLFSNLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYI3</accession>
    <name>A0A3B5LYI3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSXCOT00000016184.1">
        <property type="protein sequence ID" value="ENSXCOP00000015985.1"/>
        <property type="gene ID" value="ENSXCOG00000012075.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163737"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635:SF196">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="132"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="391" mass="44613" checksum="67384ADDEAAA1193" modified="2025-10-08" version="2">MCADSRLFLFRSKGNFSILPKEKRNWTLINEKGEEKCEEQQILTEIYDYYKNLYRYQAAQEDEMVELLRKLTDKLTHTEKSRLDQEITINEITEALISMKNNKTPGLDGLPKEFYTIFWESLKAPLLQVYREALSEGKLPNSMNQGMISLLYKKGEKTDLKNWRPLTLLGVDVKIIAKVLFFRLQTVITTIIGKEQTCGVKGRKMTDSLALIRDGYLYSQERQLSTAILGIDLEKAFDRVNHTFLERILDHFNFGETFKRWIKTLYNECSSVVVVNGTLTPPLAIKAGVRQGCPLSPLLFIIAIEPLACAIRENKMIKGITPPGNEADNASICESLKISEKFTKASGMKVNKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFETFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYW2</accession>
    <name>A0A3B5LYW2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Signal peptidase complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 9">Component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Enhances the enzymatic activity of SPC and facilitates the interactions between different components of the translocation site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Endoplasmic reticulum membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SPCS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYW2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017078"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017298.1">
        <property type="protein sequence ID" value="ENSXCOP00000017078.1"/>
        <property type="gene ID" value="ENSXCOG00000012707.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000038181"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009582">
        <property type="entry name" value="Spc2/SPCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13085">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 25 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13085:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06703">
        <property type="entry name" value="SPC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="258" mass="28768" checksum="952CA5A2D5BF87A0" modified="2018-12-05" version="1">MSDSLKKSKLCVGETRSNVVRHRPAGGHQPQLRSRHPAVVREQPLHLGAHHGAGPQAHLEGGADIIGASGSAAAAGRLSFQWRIDEKPVKIDKWDGAAVKNSLDDAAKKVLLEKYGYVENFQLVDGRLLICTISCLFAMLALVWDYLYPFPESRPVLACCVISYPSPEKNIFLMAMQKDPAGMDPDHVWQLSSSLKRFDDQYSLRVFFTDGKTRNSRDAEFTRSVSTFFDESGTLVMDHFEAYVSKLHDTLATEKKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZP5</accession>
    <name>A0A3B5LZP5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DDIT4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZP5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016642"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016857.1">
        <property type="protein sequence ID" value="ENSXCOP00000016642.1"/>
        <property type="gene ID" value="ENSXCOG00000012555.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063652"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.40">
        <property type="entry name" value="RTP801-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012918">
        <property type="entry name" value="RTP801-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038281">
        <property type="entry name" value="RTP801-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12478">
        <property type="entry name" value="DNA-DAMAGE-INDUCIBLE TRANSCRIPT 4 PROTEIN DDIT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12478:SF18">
        <property type="entry name" value="REGULATED IN DEVELOPMENT AND DNA DAMAGE RESPONSE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07809">
        <property type="entry name" value="RTP801_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="187" mass="20887" checksum="C2A12D1133BA7FC8" modified="2018-12-05" version="1">MVYIAAMPFGHGVSVLTEEESVVEMIGKYFFHHNSPGRGSSSLKTSNSSLKSDEQQLLHQDLTRQMECCLTEAKPSVLRCQVLLLPLNMITRVGHHILLSSANEPCGLRGASIRLYVEGKDGLKSVGTVVPDPNVTPTFELSVVFKVDKEDGWHIFDSGKVLKLRPEYRLMKRKLYSSESPVIHEFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0L5</accession>
    <name>A0A3B5M0L5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0L5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017866.1">
        <property type="protein sequence ID" value="ENSXCOP00000017643.1"/>
        <property type="gene ID" value="ENSXCOG00000013304.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182898"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005337">
        <property type="term" value="F:nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015862">
        <property type="term" value="P:uridine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002259">
        <property type="entry name" value="Eqnu_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332:SF9">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01733">
        <property type="entry name" value="Nucleoside_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016379">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01130">
        <property type="entry name" value="DERENTRNSPRT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="372" mass="41354" checksum="B6DF659D551C28DF" modified="2018-12-05" version="1">MTAINAPQDRYHAVWIIFFILGLGTLLPWNFFMTATMYFTSRLKDPITEHNNATSERNMLESKFNNVMTLCAMVPLLIFTCLNSFIHQRIPQKLRISGSLAAIFLVFMVTAILVKVDIEPVPFFTLTMIKIVCINSFGAVLQGSLFGLAGILPASYTTPIMSGQGLAGTFAAFSMICALASGSELQDSAFGYFVTACVVILLAITSYFMLPKMEFFQYYMESSRSAPSADEENKMDLLKKDTYFIPVSCFLLFNMMDWAGRSLTAFSMWPGKDSIWLPVLCGVRVVFIPLFMLCNVHPRENLPVVFVHDAWYIGFMILFSFSNGYLASLCMCFGPKKVPQHQAETAGAIMAFFLSLGLALGAAVSFGIRVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0R8</accession>
    <name>A0A3B5M0R8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ATP-binding cassette sub-family G member 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Sterolin-2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0R8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017693"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017917.1">
        <property type="protein sequence ID" value="ENSXCOP00000017693.1"/>
        <property type="gene ID" value="ENSXCOG00000013339.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159739"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120020">
        <property type="term" value="F:cholesterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033344">
        <property type="term" value="P:cholesterol efflux"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03234">
        <property type="entry name" value="ABCG_White"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000831">
        <property type="entry name" value="ATP-binding cassette sub-family G member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF71">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY G MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="389"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="425"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="489"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="568"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="19">
        <location>
            <begin position="28"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00073175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00075114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="603" mass="68653" checksum="CAF6951073BB9C2B" modified="2018-12-05" version="1">YSHKDTELFSSDEDSSLYFTYSGEGNELEVSNLHYEVDTSAQIPWYEKLSEFKLPWEVKGNKQTAINNLSLRVRSGQMLAVIGSSGCGKTSLLDIITCRDEGGRMTCGHVLINGKPNTPQLVKKSIAHVRQDDRLLPHLTVRETLCFVAKLRLPSHFSQAQRDQRVDDVIAELRLRQCAHTRVGNDYVRGVSGGERRRVSIAVQLLWNPGILILDEPTSGLDSFTAHNLVITLSRLAKGNRLILLSVHQPRSDIFQLFDLVVLLSSGSAVYFGAARDMVPYFTALGHPCPRYCNPSDFYVDLISIDRRSPEREAECLERSRLLSEQFKEKVQDSNDHMWKPAETSAAYIKSRNREEVVNISRDRNELPGELQQFTTLIRRHMYNDFRDLVSLLVRGFEALLMSLLVGCLYYGAGEERLNIQDTVALVYMIGALTPFAVVLDVIAKCHTERAMLYHELEDGMYSVTSYFFAKGSLLLHDRFMPFQCWNTSAFMGNALFTVFYLTGGFVISLENMWLVASWLSYASFMRWGFDGMLQVQFRGNKYPVTIGNFTINIDGIHVVEAIKLNQYPLFSCYLVLVAVCFGFMVLYYVCLKFIKQKSSQDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2C5</accession>
    <name>A0A3B5M2C5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Family with sequence similarity 167 member B</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM167 (SEC) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M2C5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015716"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015910.1">
        <property type="protein sequence ID" value="ENSXCOP00000015716.1"/>
        <property type="gene ID" value="ENSXCOG00000011872.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159693"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024280">
        <property type="entry name" value="FAM167"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051771">
        <property type="entry name" value="FAM167_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32289">
        <property type="entry name" value="PROTEIN FAM167A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32289:SF4">
        <property type="entry name" value="PROTEIN FAM167B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11652">
        <property type="entry name" value="FAM167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="82"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="183" mass="21134" checksum="0DBE42E138AEB9B3" modified="2018-12-05" version="1">ELGADCSEGDAEDLDSVKALTEKLKLQTRRPSYLEWQERLQSRPWTDRSPTDCPDSAEGDVSMPAILRNDNSELVVRNICGFDTIDDALEFLRKELREMQVQDNRLARQLIRLRGEIHRLKVEQVCHRHKEMLDDATYELEECEEESDLLCDIPMKAAFALSTPLKHLGLTKMNINSRRFSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2N8</accession>
    <name>A0A3B5M2N8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">LON peptidase N-terminal domain and ring finger 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M2N8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017682"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017906.1">
        <property type="protein sequence ID" value="ENSXCOP00000017682.1"/>
        <property type="gene ID" value="ENSXCOG00000013331.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16514">
        <property type="entry name" value="RING-HC_LONFs_rpt2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.30.130.40">
        <property type="entry name" value="LON domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046336">
        <property type="entry name" value="Lon_prtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327:SF41">
        <property type="entry name" value="LON PEPTIDASE N-TERMINAL DOMAIN AND RING FINGER PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327">
        <property type="entry name" value="RING FINGER PROTEIN 127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="52"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Lon N-terminal" evidence="7">
        <location>
            <begin position="131"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="345" mass="39917" checksum="0F694F3E0635669F" modified="2018-12-05" version="1">LKRKRRDVEEEGEPQEEISHQKKSNCGELTTLVQPCCFSLESDLWDPTDLECSLCMRLFYEPVTTPCGHTFCLQCLERCLDHNPKCPLCKEELSEYLVQRQYCKTVLMENLISKYLPSEFIERQKMHEEEIAELSNLNKSVPIFVCTMAFPTVPCPLHIFEPCYRLMIRRCMETGTNCFGMCLGDDLKGFVGYGCLLEIRDVKFFSDGRSVVDTIGRRRFKIIQHRERDGYNTADVEYLEDVKVEGIAERELQSLHDAVYDQALVWVDSLKAEQKQRIEGHFGPMPRKDSELQASPNGPSWCWWLLAVLPLEGRAQLPFLALTSLKDRLSGIRKVLLFMSQSRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3D9</accession>
    <name>A0A3B5M3D9_9TELE</name>
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        <submittedName>
            <fullName evidence="4">ABI family, member 3 (NESH) binding protein b</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3D9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014674.1">
        <property type="protein sequence ID" value="ENSXCOP00000014489.1"/>
        <property type="gene ID" value="ENSXCOG00000010860.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063558"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010811">
        <property type="term" value="P:positive regulation of cell-substrate adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
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        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049109">
        <property type="entry name" value="TARSH/FNDC1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23197:SF10">
        <property type="entry name" value="TARGET OF NESH-SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23197">
        <property type="entry name" value="TARSH-RELATED FIBRONECTIN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21731">
        <property type="entry name" value="TARSH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017227191" evidence="2">
        <location>
            <begin position="27"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="120"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="375"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="607" mass="67822" checksum="FC044A887EEC54CF" modified="2018-12-05" version="1">VMVALLLRVLLLLLIGGTVLLGGSPAQRIRVRRQNMKVRINATGDTIVMKFVRPGPDVKLEGYILGYGRSVFSKQFIQLPENGQLYETELDAEPKYLVAVQPIPVNDMKKHCTGKVNLEKPLHLVIGSISPTAVLLSWGNHLKTPYEKNVMSECLEDGFYTIRYRERNRKWIYQTCPASDTVIDHLKPDTQYEFGVRSNKDDRSSLWSKPVIHSTNTAKNLERPVKAPASWLLVNRPEKSRNPEIPKYKVPAVTTKPTKQERRQTTTTAAPTAGESSEPQRHGEGLEGIRKLEGSGSATSSNMQQQAATSSTSGINRTSVLLPVELIEPFRPAAPHVVYQTGKKPDEPCSITSSLNYFPEDEPGEVNVTSPPTSPPANLTVVTVEGCPSFIILDWEKPDNHTTEYEVISTAKGPDGERVSILTTNQTHTAVENLNPESSYEFTVKPKNELGEGPVSEPVSFNTESADPRVSENVSGKDAIWTQFPFKTDSYSDCHGKQYVKRTWYRKFVGVQLCNSLRYKIYLSDALNGKFYHIGDQSGFGEDHCQFVDSFLDGRTGRQLRADQLPSRNGFFRALRQEPVHFGQIGGHSHINYVSWYECGTPIPGKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4N1</accession>
    <name>A0A3B5M4N1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 24">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="6 24">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21 24">Lysyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22 24">
            <text>tRNA(Lys) + L-lysine + ATP = L-lysyl-tRNA(Lys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="6 24"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4N1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014929"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015115.1">
        <property type="protein sequence ID" value="ENSXCOP00000014929.1"/>
        <property type="gene ID" value="ENSXCOG00000011313.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234618"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003877">
        <property type="term" value="F:ATP:ADP adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002276">
        <property type="term" value="P:basophil activation involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015966">
        <property type="term" value="P:diadenosine tetraphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043032">
        <property type="term" value="P:positive regulation of macrophage activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00775">
        <property type="entry name" value="LysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04322">
        <property type="entry name" value="LysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000050">
        <property type="entry name" value="Lysine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000029">
        <property type="entry name" value="Lysine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00252">
        <property type="entry name" value="Lys_tRNA_synth_class2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002313">
        <property type="entry name" value="Lys-tRNA-ligase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034762">
        <property type="entry name" value="Lys-tRNA-ligase_II_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044136">
        <property type="entry name" value="Lys-tRNA-ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00499">
        <property type="entry name" value="lysS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001756">
        <property type="entry name" value="PRK00484.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918:SF9">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918">
        <property type="entry name" value="LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039101">
        <property type="entry name" value="LysRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0007">Acetylation</keyword>
    <keyword evidence="19" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0436">Ligase</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="26">
        <location>
            <begin position="214"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00030563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00059215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU003748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="559" mass="63775" checksum="893761B1BAAFE7CD" modified="2018-12-05" version="1">RRMKADKKAAEKESKGKDLGEQTDSNNANAQNASALDDETLDPNQYFKIRTQAIQELKGTAEDPYPHKYHIDLSLTDFIQKYNHLQPGDQLSDVVLAVSGRVHAKRASGAKLIFYDLRGEGVKLQVMANSRNYKSEEAFVAINNKLRRGDIIGVRGNPGKTKKGELSIIPIEMTLLSPCLHMLPHLHFGLKDKETRFRQRYLDLILNDYVRQKFLTRAKIITYLRSFLDQMGFLEIETPMMNIIPGGAVARPFVTYHNELDMNLFMRIAPELYHKMLVVGGLDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKLLSGMVKHITGGYKVTYHPDGPEGQAYEVDFTPPFKRVSMTHDLEKIMGVKFPPTDSYDSDETRKFFDDLCAQKGVECPPPRTTARLLDKLVGDFLEVTCISPTFICDHPQIMSPLAKWHRSEKGLTERFELFVMKKEICNAYTELNDPIRQRELFEQQAKAKAEGDDEAMFIDETFCTALEYGLPPTAGWGMGIDRLTMFLTDSNNIKEVLLFPAMKPDDNKKSAPSEETSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M574</accession>
    <name>A0A3B5M574_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily domain-containing protein 4A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M574"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018826.1">
        <property type="protein sequence ID" value="ENSXCOP00000018592.1"/>
        <property type="gene ID" value="ENSXCOG00000013993.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063320"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000200">
        <property type="entry name" value="Major facilitator superfamily domain-containing protein 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23121:SF10">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23121">
        <property type="entry name" value="SODIUM-DEPENDENT GLUCOSE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="396"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="429"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="457"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="492" mass="54034" checksum="F42BE42B050D032B" modified="2018-12-05" version="1">MFIDERIVTLFKRNAHHTLTYWSVFFSFGLCIAFLGPTILDLQCQTNSTLSQITWVFFSQQFCLLIGSSVGGVFKRTLFSALAALFVSALLISVIFAIIPVCNNVLILAIAMAVSGLAMGVIDTIANIQLVTIYQKDSAIFLQALHFFIGFGALVSPLIADPFLSETGCGNHTGNETEIMHHFRSMLRYSPIAEHNITQSHLPHDGVEKEEPIVQYAFWIMALINLPVPIAVLFLMYRENLLPCCPGSSPRLLDKDELAMETQQGAEGPDLEQKEHEAGGHGSIFSCCQNDNLHGLPVSFFMIHILGGMVLFMTDGIVGAYAGFVYTYAVAPPMSLPNKTAGYLASIFWGAITAGRLLSIPLSYRFQPVRLLVFNLAGAFVTVLMLLIFYTSSIFLFVGTCLCGLFLSSIFPCMLAYTEDILDYQGCATSVLVTSAGMGEIVMQVLVGSIIQTEGSYSFLLCGMIIACFGFILFFGLLLFHRMHRNYLTGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5G0</accession>
    <name>A0A3B5M5G0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CBF1-interacting co-repressor CIR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC25 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M5G0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015219"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015410.1">
        <property type="protein sequence ID" value="ENSXCOP00000015219.1"/>
        <property type="gene ID" value="ENSXCOG00000011533.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000039055"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="CIR_N_dom"/>
    </dbReference>
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        <property type="entry name" value="CWC25_splicing_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16196">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16196:SF0">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC25 HOMOLOG"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cir_N"/>
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        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="11"/>
            <end position="47"/>
        </location>
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        <location>
            <begin position="23"/>
            <end position="57"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00006695"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01083"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015219.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    </evidence>
    <sequence length="64" mass="7661" checksum="FE76A82031A05D01" modified="2018-12-05" version="1">MGGGDLNLKKSWHPQTMKNIERVWKAEQKYEAERKKIEELQKELKEERAREEITRYAEETGAIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7F9</accession>
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        <submittedName>
            <fullName evidence="3">Glycerophosphodiester phosphodiesterase 1</fullName>
        </submittedName>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:glycerophosphodiester phosphodiesterase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006580">
        <property type="term" value="P:ethanolamine metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:N-acylethanolamine metabolic process"/>
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        <property type="term" value="P:phospholipid metabolic process"/>
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        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
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    </dbReference>
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        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE 1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR46320:SF1">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE 1"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="63"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS51704"/>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019432.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="329" mass="37174" checksum="643933390EC1FC7E" modified="2018-12-05" version="1">MLQLGDEVTLYSLVFVLVLLGTRSPLWSTALTTSLYLFVAMFRFPQLATSRARQVLNPAAGKASAVAHRGGGHDAPENTIAAIREASRNGAAGVELDLEFSADGIPILMHDDTVDRTTNGSGPLNKMSFSELLKLDASAKHRFRSKFVGEKIPTLEEAVKECIKLKLTIYFDVKGHPDESAAALKDLYKKYPVLYNSSIVCSFQPKVIYRMRQSDPEVLTALTHRPWSLSCLGDGTPRFSSLWQHHWMRMMDILLDWAHHHLLWKLCGTSAFLIQKNVVSQDYVQYWAQRGVEVVAWTVNTKLEKDYFQEVLHINYITDSLVEDCEPHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M922</accession>
    <name>A0A3B5M922_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ubinuclein 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ubinuclein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M922"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016254"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016460.1">
        <property type="protein sequence ID" value="ENSXCOP00000016254.1"/>
        <property type="gene ID" value="ENSXCOG00000012285.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="HRD"/>
    </dbReference>
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        <property type="entry name" value="UBN_middle_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21669">
        <property type="entry name" value="CAPZ-INTERACTING PROTEIN AND RELATED PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21669:SF10">
        <property type="entry name" value="UBINUCLEIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08729">
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    </dbReference>
    <dbReference type="Pfam" id="PF14075">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hpc2-related" evidence="4">
        <location>
            <begin position="146"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Ubinuclein middle" evidence="5">
        <location>
            <begin position="408"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="37"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
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            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="529"/>
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        </location>
    </feature>
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        <location>
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            <end position="724"/>
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    </feature>
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        <location>
            <begin position="765"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="823"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="870"/>
            <end position="983"/>
        </location>
    </feature>
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        <location>
            <begin position="1059"/>
            <end position="1086"/>
        </location>
    </feature>
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        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
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        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
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        <location>
            <begin position="304"/>
            <end position="318"/>
        </location>
    </feature>
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        <location>
            <begin position="530"/>
            <end position="539"/>
        </location>
    </feature>
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        <location>
            <begin position="700"/>
            <end position="724"/>
        </location>
    </feature>
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        <location>
            <begin position="778"/>
            <end position="797"/>
        </location>
    </feature>
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        <location>
            <begin position="887"/>
            <end position="899"/>
        </location>
    </feature>
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            <end position="1074"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    <sequence length="1086" mass="116202" checksum="6E54FC756BC803D7" modified="2018-12-05" version="1">MAEPRKVQFVTLSAFAAGTAAESRKRRLDEEADFNFDRAGEAEAGGGAGGSASNGRLGKTSDRDKAAAERRATVRLNLSLSEPDERTSAEFNYGELIQNLQNKSNLPSLTSSLNRLNDDEEERERLEVEALAKKFENKYGNTGMKKRKDRMQDLIDIGFGYDETDPFIDNSEAYDELVPASLTTKLGGFYINTGTLQFRAASDSEGEEDKVRQEQAMKKRKKIEVNCVEEKNLTMNKVTKQGVTTLNLQRPEKKKRKKLMKDSLYLAAMLRRFTREKEEIKKRNPSIDHLRLAAGTASKPPNPGNATQLPLASNSTHLQGGASTGNLPLTDLTSDPAVMSLLGSANEKELQDLLGDLDFSLLDTDQQHAVVTARENGIMGIGVPSSKAVGGSGQGRSLASSGGLFSPLPDGLPAPLIKRIEDLRAASRQFDQEGRKKFFTLDMNNILLDIELQVQEQPPGIRSDIYSHMEAFVPCNKEALLKRLKKLSLNIQDDRLRAPLLKLKLAVCSAMPEQIAKYNMECMAKVAKHQSGEGDRNGSEEEDDEKPGKRVMGPRKKFIWDDMLRTLLCNLVRVKLSCYEMENQCTLSVEDYLKAFLENEVKPLWPKGWMQTRILLKESLDGLWINKLPLTMTSNLAVTSARQPLLMASPPEPICLSDSLDEDLTAPSLDSISHALALLSNASKGLGLADPTPLPPPPAASVGNVSGTSSSTSSSSSTPPVVSSMVRLHTPGLSLASRTADCHYGQVVKGCLAQTQRHVTMATPSHRPAAAMGKMHPHPSPSPPKQRPPPTASPLLPPHQKGFTCPAGILGTLGTLGGVSKSTVKPSGGVSSSNGGGTLSGPVQPPSSSSKPQSHHHHQANFITPMQATLTKSSLSSNSSPIIKLTPRPPVPTPPPSSSPSPSSSPSHQLSHQQHQYASKTPKTFRTPFSVSGGGPVKPVPPVSCSFIQKPQPPPSSSANSKGDKTPPSSTASVSANQRQRVSVGGVIQSSKAGNGWAPSGTLATSATTSHLSQVSAAGSPLLTNHSALPLGFGMMGGLVPVSLPFQFPPLLNFSAPGAASAASMGSTPSSNSGYPLAQRSGKRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9D2</accession>
    <name>A0A3B5M9D2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription factor SOX-10</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
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    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9D2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017896"/>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9S4</accession>
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        <name type="scientific">Xiphophorus couchianus</name>
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    </dbReference>
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        </location>
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    </evidence>
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    </evidence>
    <sequence length="416" mass="49033" checksum="E1DEEFC69489B571" modified="2018-12-05" version="1">VNDDIQIGDSITDMMQKALFDFLKHRFEGRITITRVTADISLAKRSILNNPGKKALMDRSNTRSNLAQIQGEVERIFELARSMQLVVLDADTINHPLQVSKTSLAPILVYVKISSPKVVFNKLTPALNIITHMHPGEMFDVILDENQLTDACEHIADYLESYWRATHPPEMEPSNALVAQLAENTLPSSPTTPGASKRKGSRENHLDKETPPAPDQEDRVEEGQRKEEEERLLRTEAKRSQHTHHHHHHHHHRRMEHHSYAQHSQISGGMNVKSGRDYSQRPPRRPLHEEREEEVEEVVVEGDEEEIHYNHRGHTHHHHSSNHHHHHHHHQRHRGQLHDREGYEQEHNERNRHQRHHQHIPTRTHHADLYPAHNHHSYHHQGRDQDRDRYKDRESDRDRIRDRDARQWHKDSYMDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCM8</accession>
    <name>A0A3B5MCM8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein 184bb</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027897940.1">
        <property type="nucleotide sequence ID" value="XM_028042139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCM8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021372"/>
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        <property type="protein sequence ID" value="ENSXCOP00000021372.1"/>
        <property type="gene ID" value="ENSXCOG00000015999.1"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00940000153861"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="379"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="417" mass="47269" checksum="9F4783896F2D01EE" modified="2018-12-05" version="1">MSQLWWRAKRLSERLGSDSPDGLPPGIPSTVAPQGSNSSWAPIPLVFRPEEPFFLMTSTAQIISGFFVWTALLITCHQIYMHLRYYSSPNEQRHIVRILFIVPIYAFDSWLSLLFFTNEEYYVYFDTVRDCYEAFVIYNFLSLCYEYLGGESAIMAEIRGKPIESSCMYGTCCLWGKAYSIGFLRFCKQATLQFCVVKPLMAVITVILQAFGKYKDGDFNVASGYLYVTIIYNISVSLSLYALFLFYFATRELLVPYSPVLKFFMVKSVIFLSFWQGMLLAILEKCGAIPQINSADFSVGEGTVAAGYQNFIICIEMFFAAVALRHAFTYRVYMDKRLDSYGRGAPMKSISSSLKETMNPGDMVQDAIHNFSPAYQQYTQQSTLERGGGPSLSRSHSNLSARGDNEKTLLLSSDDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MD84</accession>
    <name>A0A3B5MD84_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MD84"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017664"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017888.1">
        <property type="protein sequence ID" value="ENSXCOP00000017664.1"/>
        <property type="gene ID" value="ENSXCOG00000013319.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154738"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:calcium-independent phospholipase A2 activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185:SF1">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="147" mass="16720" checksum="348783F33BAF48A8" modified="2018-12-05" version="1">MNNPCTLAVHESRLMWPNTPFQCVLSLGTGRYDDVKRSPSTFTSLRANINNLIFSATDTEGVHTLLGDLLPPDVYFRFNPMMNADVSLDENHPETLDKLVTDTQDYLNINRSKVAKLCLVLSTQRSSMSQAKDWLSQRTWEIKKTLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MD91</accession>
    <name>A0A3B5MD91_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase M41 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MD91"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022233"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022502.1">
        <property type="protein sequence ID" value="ENSXCOP00000022233.1"/>
        <property type="gene ID" value="ENSXCOG00000016609.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160625"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005745">
        <property type="term" value="C:m-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF9">
        <property type="entry name" value="AFG3-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M41" evidence="8">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="131"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="134"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="151"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="182" mass="20739" checksum="07E9A85028A00133" modified="2018-12-05" version="1">MCMTLGGRVAEEIFFGRVTTGAQDDLRKVTQSAYAQIVQFGMNPKVGQVSFDLPRQGEMVLEKPYSEATARLIDSEVRTLIGEAYRRTQLLLTDKKAEVDKVAQRLLQREVLSKEDMEELLGRRPFAERSTYEELVEGTGGEDEDTALPDGLRDWNQDRNQDRNQESPEEQVAQKIRTQADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5ME04</accession>
    <name>A0A3B5ME04_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000017914"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018138.1">
        <property type="protein sequence ID" value="ENSXCOP00000017914.1"/>
        <property type="gene ID" value="ENSXCOG00000013494.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156887"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
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        <property type="term" value="F:hydrolase activity"/>
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        <property type="term" value="P:nervous system development"/>
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        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
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        <property type="entry name" value="probable global transcription activator SNF2L2 isoform X1"/>
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        <property type="entry name" value="Putative global transcription activator SNF2L2"/>
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        <property type="entry name" value="SNF2-related domain-containing protein"/>
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        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Tandem AAA-ATPase domain"/>
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        <property type="entry name" value="BRG1_ATP-bd"/>
    </dbReference>
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        <property type="entry name" value="BRK_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037259">
        <property type="entry name" value="BRK_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
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        <property type="entry name" value="Bromodomain-like_sf"/>
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        <property type="entry name" value="Bromodomain_CS"/>
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        <property type="entry name" value="Gln-Leu-Gln_QLQ"/>
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        <property type="entry name" value="Helicase_ATP-bd"/>
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        <property type="entry name" value="Helicase_C-like"/>
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        <property type="entry name" value="HSA_dom"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="SNF2-like_sf"/>
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        <property type="entry name" value="SNF2/RAD54-like_C"/>
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        <property type="entry name" value="SNF2_N"/>
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    <dbReference type="PANTHER" id="PTHR10799">
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        <property type="entry name" value="BROMODOMAIN"/>
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        <property type="entry name" value="BROMO"/>
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        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HELICc"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="BROMODOMAIN_1"/>
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    </dbReference>
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        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HELICASE_CTER"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="10 15" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="QLQ" evidence="21">
        <location>
            <begin position="163"/>
            <end position="198"/>
        </location>
    </feature>
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        <location>
            <begin position="391"/>
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            <begin position="693"/>
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            <begin position="1011"/>
            <end position="1173"/>
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            <begin position="1357"/>
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            <begin position="1"/>
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            <begin position="1254"/>
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            <begin position="1292"/>
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        <location>
            <begin position="1444"/>
            <end position="1532"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
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        <location>
            <begin position="36"/>
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        </location>
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        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
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            <begin position="126"/>
            <end position="136"/>
        </location>
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    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
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            <end position="230"/>
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            <begin position="1506"/>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
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    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
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    <evidence type="ECO:0000259" key="18">
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    </evidence>
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            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51666"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1532" mass="173922" checksum="4028B03A91BC3530" modified="2018-12-05" version="1">MSTPDPPMGGTPRPGPSPGPGPSPNAMLGPSPGPSPGSSHSMMGPSPGPPSSGHSQPVPSGYGPENMHPLHKPMEGMHEKSLCDDSRFSQMKGLSMRQGGHSGMGPPPSPLDQHSQGYHSPLGGSDHSSPVASNGPPSGPLIVPSLGSGLDGSGPGGPGGPTPFNQNQLHQLRAQIMAYKMLARGQPLPDHLQMAVQGKRPMPGMQQQQPMSSLVSGTSAGPGAAPSNAPQKLIPPQPTGRPSPAPPSVPPAASPVMPPQTQSPGQPAQPPPMMPYHAKQNRITPIQKPCGLDPVEILQEREYRLQARIAHRIAELENLPGSLAGDLRTKATIELKALRLLNFQRQLRQEVVVCMRRDTALETALDAKAYKRSKRQSLREARITEKLEKQQKIEQERKRRQKHQEYLNSILQHAKDFKEYHRSVTGKMQKLTKAVSTYHANTEREQKKENERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAYLLQQTDEYVANLTELVRAHKAAQALKEKKKKKKKKKKLENAEGQTPALGPDGEPLDETSQMSDLPVKVIHVDSGNILTGVDAPKAGQLETWLEMNPGYEVAPRSDSEDSEEEEEEEEEEEEPQPSSAPVEEKKKITDPDSEDVSEVDVRHIIENAKQDVDDEYSGAAFARGLQSYYSVAHAVTEKVEKQSTLLINGQLKQYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTIALITYLMEHKRLNGPYLIIVPLSTLSNWVYEFDKWAPSVVKVSYKGSPAARRAFVPQLRSGKFNVLLTTYEYIIKDKQVLAKIRWKYMIVDEGHRMKNHHCKLTQVLNTHYLAPRRVLLTGTPLQNKLPELWALLNFLLPTIFKSCSTFEQWFNAPFAMTGEKVDLNEEETILIIRRLHKVLRPFLLRRLKKEVEAQLPEKVEYVIKCDMSALQRVLYRHMQAKGVLLTDGSEKDKKGKGGTKTLMNTIMQLRKICNHPYMFQQIEESFSEHLGFSGGIVQGPDLYRASGKFEVLDRILPKLRATNHKVLLFCQMTTLMTIMEDYFAYRNFKYLRLDGTTKAEDRGMLLKTFNEPGSEYFIFLLSTRAGGLGLNLQSADTVIIFDSDWNPHQDLQAQDRAHRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQKSSSHERRAFLQAILEHEEQDEEEDEVPDDETVNQMIARSEEEFEQFMRMDLDRRREEARNPRRKPRLMEEDELPTWIMKDDAEVERLTCEEEEEKMFGRGSRQRKEVDYSDSLTEKQWLKAIEEGTLEEMEEEVRHKKTTRKRKRDRDRDDDGKRQRKRGRPPAEKLSPNPPGLTKKMRKIVDAVIKYKDSTSGRQLSEVFIQLPSRKELPEYYELIRKPVDFRKIKERIRSHRYRSLGDLERDVMLLFQNAQTFNLEGSLIYEDSIVLQSVFTSLRQKIEKEEESEGEESDEEEEEQEDGSESESRSVKVKIRLSRKDKGGDRGKGRSRRTGRTRAKPVVSDDDSEDEQEEVTKAVCGHALQHNKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5ME19</accession>
    <name>A0A3B5ME19_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox protein aristaless-like 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Binds DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5ME19"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022154.1">
        <property type="protein sequence ID" value="ENSXCOP00000021887.1"/>
        <property type="gene ID" value="ENSXCOG00000016360.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159662"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000127">
        <property type="entry name" value="homeobox protein aristaless-like 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050649">
        <property type="entry name" value="Paired_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329:SF322">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 13 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 13 14" id="KW-0371">Homeobox</keyword>
    <keyword evidence="10 13 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="166"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="17">
        <location>
            <begin position="342"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="13">
        <location>
            <begin position="168"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="110"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="362" mass="39804" checksum="EEBB110401063DB1" modified="2018-12-05" version="1">MNADTCVSYCDMTSMDSYYSASTAQSRDHQANSFRTFQTTETKYSPTFIPGKGSRSPFQTECQSLDGTEESNFNKYQLFMQRPTCKTPPDGGKLHPDSGHNGTLIPCYGKDHSGLTESELPPSVDPPGMEGSYLNLKEPVVKGPSERSGSDLSSPLEKSEAESNKGKKRRNRTTFTSYQLEELEKVFQKTHYPDVYAREQLALRTDLTEARVQVWFQNRRAKWRKRERFGQMQQVRTHFSTAYELPLLTRPENYAQIQNPSWIGSSSAASPVPGCVVPCDSVPSCMAPHPHAPGGVSDFLGVPSPGSSHMGQTHMGSLFGGAGIGGGINGYDLNVDPDRKSSSIAALRMKAKEHSAAISWAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEP3</accession>
    <name>A0A3B5MEP3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Formin-like 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEP3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022030"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022297.1">
        <property type="protein sequence ID" value="ENSXCOP00000022030.1"/>
        <property type="gene ID" value="ENSXCOG00000016444.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156292"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2220:FF:000001">
        <property type="entry name" value="Formin-like 1, isoform CRA_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000036">
        <property type="entry name" value="Formin-like protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010472">
        <property type="entry name" value="FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043592">
        <property type="entry name" value="FMNL_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857">
        <property type="entry name" value="FORMIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857:SF2">
        <property type="entry name" value="FORMIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="3">
        <location>
            <begin position="19"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="4">
        <location>
            <begin position="595"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="148"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="413"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="486"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="157"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="486"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="511"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1010" mass="113088" checksum="9674810807228712" modified="2018-12-05" version="1">WAKIPPQKSKEIGKGSNIHFSIKFLKNYFSFSFLQSYMNLPPDKLKLLSQYDNDKKWELVCDQERFQVKSPPSTYLSKIKSFYQDQGGVSRRKKIQDATQVLKALEISLRTNHIGWAQEFLNEQNKGLDVLVEYLSHAQSDTSFDLETVENGGTLSDRQKPAERSMEDLTKSSSGGHSHGMSRAARALTVRISSTLANRMHKKSHLSQRRDDIHVCIMCLRAIMNYQSGFNLVMTHPRCVNEITLSLNSKNPRTKALVLELLAAVCLVRGGHDIILSAFDNFKEVSKERNRFEKLMEYFINDDNNIDFMVACMQFINIVVHSVENMNFRVHLQYEFTHLGLDKYLESLKQTESEKLQVQIQAYLDNVLDVGALLEDAENRGGVLEHVDELQNHNIHVKYYILHNSYLRESCSQVSSLQQRERERELDRERERDRERRGSSVGQSHSELEQKIQELQHKGVIKLGRTPSGGLDIQVVPVKVVEYVQSPSSSTEPSIQTSAPTTAGGPGETATPPPPPPPPSDGSSVAPPPPPPPPPPPPPGAGPPPPPPPPPPPPGAGPPPPPPPPGAGPPPPPPPPGGGPPPPPGAPNAQSGFKTKKPIQTKYRMPLLNWQALKPNQVTGTVFNELDDEQILGELNMETFEEQFKTRAQGNSADLAKVKKKVAQKAPTKTSLIDANKAKNLAITLRKGGMDPSAICTAIETYDQQSLSIDFLELLEHFIPSDFEMKLLVNYEKEGRPLEELTKEDQFILHFGKIARLNQRINTLTFMGNFPESVKRLQPQLNSIIAASMSIKSSAKLKKILEIVLAFGNYMNSSKRGAAYGFRLQSLDLLLETKSTDRSQTLLQFITNIVLEKYPDLVNFHTDIHFIDKAALVSLDSILQDIRSLERGMEMTKKEFLVQDDSPVLKEFIKTNSEHLESLIKDSKTAQEAYASVVEYFGENPKTTQPSMFFPLFGRFIKAYKGPMMPKMDLIAELKKRQVKSPVREGKDGALEDIITGTVTRNTVEFYTKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEX7</accession>
    <name>A0A3B5MEX7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">3'-5' exoribonuclease 1</fullName>
            <ecNumber evidence="5">3.1.13.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Histone mRNA 3'-exonuclease 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.13.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.13.1" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEX7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019756"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020002.1">
        <property type="protein sequence ID" value="ENSXCOP00000019756.1"/>
        <property type="gene ID" value="ENSXCOG00000014835.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063205"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008859">
        <property type="term" value="F:exoribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06133">
        <property type="entry name" value="ERI-1_3'hExo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.30:FF:000015">
        <property type="entry name" value="3'-5' exoribonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000034">
        <property type="entry name" value="3'-5' exoribonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051274">
        <property type="entry name" value="3-5_Exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047201">
        <property type="entry name" value="ERI-1_3'hExo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044">
        <property type="entry name" value="3'-5' EXONUCLEASE ERI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044:SF61">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="15" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="7" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="SAP" evidence="21">
        <location>
            <begin position="64"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Exonuclease" evidence="20">
        <location>
            <begin position="119"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="323"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00070944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00080754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="334" mass="38514" checksum="4F6E3AE9A71CC306" modified="2018-12-05" version="1">MDEHKENIQRTDVNVKMSEEKEDEKTKPRSRICPDDETAPETSQSNKDFSHPVYKEIALANGHINRMSKDELRVKLSELKLDTRGVKDVMKKRLKNHYKKQKLLQSAAEGGPTDTYYDYICVVDFEATCEEDNPSDFLHEIIEFPMVLINTHTLQIVDTFQAYVKPEVNPQLSDFCVKLTGITQETVDGADPFPDVLHRVVEWLQERELGTKYKYAMLTDGAWDMSKFLNIQCRISRIRYPQFAKKWINIRKSYCNFYKTKLSTMLEKLGMKYEGRPHCGLDDSHNIARIAVRMLQDGCQLRVNERMHGGQLLSVPSSAPVEGAPPPRNPGNRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MF43</accession>
    <name>A0A3B5MF43_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glutamate decarboxylase like 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MF43"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022908"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023184.1">
        <property type="protein sequence ID" value="ENSXCOP00000022908.1"/>
        <property type="gene ID" value="ENSXCOG00000017115.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158391"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06450">
        <property type="entry name" value="DOPA_deC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000016">
        <property type="entry name" value="Glutamate decarboxylase like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.170">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45677:SF1">
        <property type="entry name" value="ACIDIC AMINO ACID DECARBOXYLASE GADL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45677">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="454" mass="51197" checksum="53DFC9E16E36274B" modified="2018-12-05" version="1">IVDNDPLDDPPLDFAATESFLRQAMTMILEDAVCEWHPPEELKELLDLELRDGGESEARILELCRDAIRYSVKPTHPHFFNQLFAGLEPYSLVASFITEALKPSLYTYEVSPVFTLMENAVLKKLIQTVGWEEGGDGLFNAGGSMSNMYAMNLARYRHFPEIKELGNSAAPRLAVFTSQECHYSISKAAALMGIGTKNVFVVPSDKRGKMIPSALEEQIKSVKSEGGVPFIVNATAGTTVLGAFDPIDEIADICEKYKLWLHVDACWGGAVLMSNKHKLLMKGIHRVDSVAWNPHKMLMACLQCSAFLVRDKTRLLQRCHSARASYLFQQDKFYNLSYDTGDKSVQCSRKPDAFKFWLMWKALGASELEQRVDRALAMARYLADEITKREGFRLLMEVAPVVKERMMKKGSMMVGYQPHRGQANFFRMVVISPQVSRQDMDFVLDEIHSLGKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHW1</accession>
    <name>A0A3B5MHW1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Molecule interacting with CasL protein 1</fullName>
            <ecNumber evidence="7">1.14.13.225</ecNumber>
            <ecNumber evidence="6">1.6.3.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-methionyl-[F-actin] + NADPH + O2 + H(+) = L-methionyl-(R)-S-oxide-[F-actin] + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:51308"/>
            <dbReference type="Rhea" id="RHEA-COMP:12953"/>
            <dbReference type="Rhea" id="RHEA-COMP:12956"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.225"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>NADPH + O2 + H(+) = H2O2 + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:11260"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.6.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Mical family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.225" evidence="7"/>
    <dbReference type="EC" id="1.6.3.1" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHW1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020711"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020964.1">
        <property type="protein sequence ID" value="ENSXCOP00000020711.1"/>
        <property type="gene ID" value="ENSXCOG00000015525.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159117"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120501">
        <property type="term" value="F:F-actin monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016174">
        <property type="term" value="F:NAD(P)H oxidase H2O2-forming activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000004">
        <property type="entry name" value="protein-methionine sulfoxide oxidase MICAL2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022735">
        <property type="entry name" value="bMERB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057494">
        <property type="entry name" value="Rossman_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF35">
        <property type="entry name" value="[F-ACTIN]-MONOOXYGENASE MICAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12130">
        <property type="entry name" value="bMERB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25413">
        <property type="entry name" value="Rossman_Mical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01203">
        <property type="entry name" value="DUF3585"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51848">
        <property type="entry name" value="BMERB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="18 26" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="21" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="17 25" id="KW-0440">LIM domain</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="14" id="KW-0521">NADP</keyword>
    <keyword evidence="15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 25" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="28">
        <location>
            <begin position="491"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="29">
        <location>
            <begin position="650"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="BMERB" evidence="30">
        <location>
            <begin position="989"/>
            <end position="1137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="743"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="847"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1125"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="26">
        <location>
            <begin position="1002"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="859"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="916"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="1125"/>
            <end position="1140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00044245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS51848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1154" mass="130453" checksum="001D54BA3891158E" modified="2018-12-05" version="1">MANKEPVNPSHATFDLFVQAKTCQEVKKHFTELCRQLQLDPKDYSNFYTKLKESLNYWKAKAVWTKLDKRASQDVYEQGKACAQNKCLVLGAGPCGLRTAIELSLLGAQVVVLEKREEFTRNNVLHLWPFTIYDIRELGAKKFYGKFCSGSLDHISIRQLQLILLKVCLILGVEVHTGVEYKGLIEPSVEKGWMAKLEPPSHPAAAFQFDVFISAGGGRFVPEGFKHKGLRGQLAIGITANFVNRNTAAEIQVPEISGVARIYNQKFFQDLLNETDIDLENIVYYKDDTHYFVMTAKRKSLLKRGVIKQDYSDADQLLAPANVDHAALYLYAHDAAYFSTGGQLPDLQFALNPAGQRDVAMFDFTCMHRAEHASLVRERRGKKLLIALVGDCLMEPFWPLGTGIARGFMASFDTAWTVRSWGMGVQHMTVIAERESMYQLLSQTTTENTSKKYPLYSIDPKTRYQRINLSAIHTRQVKKPFIKAEGKSDTMSGFNELLRWCQNSTRGYDNVNIKDFSQSWKSGLALCALIHHFKPHLIDISSLDEANRVHNNQLAFSVINKELGIPPVMSPSSQIDKLSMVVYLTQIKDAVTLPPKGPLPPSTSQSLSRTQSAVFFLSKLKHLSLQKQKVTAASPPPEPEPAEILTTDNEQCYFCGKMVYAVERISAEGRFFHRSCFTCHSCGITLRLGGYAFDKNSGELSIVWRFYCEMHSEDLLLTDGVEASYSVSSTHSDDQEEPDQIIHHSKGDSQEPQNPSTFPDPAAKPEILEPTQDEAVTSPVPKPRLSRQTTPTCEPSPPVAKPRKVVLTPQSPFLEAAPEETSKVGPDVSLKPLRKLQLSVEERNELGNLQSFSADSDSETQGGSSSCSSSSANAGGPPKQEGQDGQEEEGYWSGSTASLIREKKNRHCFKRKEMPSGQNRVRSKFSPWNLSSPRISRDCRLTVHPGRVGMWIPDLLLQGKTYFTQYSLEPSDPVQAKKFELKRMKTLQRRAKMSIMDRFCKAQSIQRRLEEIEVSYKDLEEKGVKLERSLRRQPNDNGSSETIEEWIQLVHEKNALVSEESDLMVESRQLELEDKRRTLELEYRKLMEIEEKTPEQEEEENQIFQQILEVVEMMDSLVTFLDEKRQKEMSENEEALSIKEAKRHSKAGAQVQWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MI18</accession>
    <name>A0A3B5MI18_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Platelet-derived growth factor subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">PDGF-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Platelet-derived growth factor B chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Platelet-derived growth factor beta polypeptide</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Antiparallel homodimer; disulfide-linked. Antiparallel heterodimer with PDGFA; disulfide-linked. The PDGFB homodimer interacts with PDGFRA and PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with XLKD1. Interacts with LRP1. Interacts with SORL1 (via the N-terminal ectodomain). Interacts with CD82; this interaction inhibits PDGFB-mediated signaling pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the PDGF/VEGF growth factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MI18"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023958"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024243.1">
        <property type="protein sequence ID" value="ENSXCOP00000023958.1"/>
        <property type="gene ID" value="ENSXCOG00000017890.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157367"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005161">
        <property type="term" value="F:platelet-derived growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048008">
        <property type="term" value="P:platelet-derived growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000041">
        <property type="entry name" value="Platelet-derived growth factor beta polypeptide b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
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        <property type="entry name" value="PD_growth_factor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000072">
        <property type="entry name" value="PDGF/VEGF_dom"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11633">
        <property type="entry name" value="PLATELET-DERIVED GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11633:SF2">
        <property type="entry name" value="PLATELET-DERIVED GROWTH FACTOR SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04692">
        <property type="entry name" value="PDGF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50278">
        <property type="entry name" value="PDGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017262278" description="Platelet-derived growth factor subunit B" evidence="12">
        <location>
            <begin position="26"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Platelet-derived growth factor (PDGF) family profile" evidence="13">
        <location>
            <begin position="72"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="306"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="424"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="360"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="424"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="447"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="465"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046967"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="476" mass="53964" checksum="0C7053308EC2BAD5" modified="2018-12-05" version="1">GSKMSSWVQLLLLAWLAACARFGVAEEDALPAALVELVRNSPINSIEDLQLLLLTDSVDEEEANSAANGGHRLPRSLDAQPAQQAQCKVRTEVVEVTRAMLDRSNANFLLWPPCVEVQRCSGCCNTKSLQCVPVVTHTRYLQVMKIEYINKRPTYAKAVVSVVDHVECRCQPAPRPTVLKKKSSHRLHSYPHRNQTLDELHQLDELKPNQRAHLEHLLGQRWDLRGDTFPQPGEGYSLAGEDVSRSEETVLFPPHWDHNFTRLLGTEEQIETHENVGMKHDEVFKDNAGPPAGNVDVEAKTKLGEDGFNITEGNPKVGHKHTQTHSPPGLDDKSKPFESHTAGLSEGFAENSRAMLDPSEETRARSRVRPTKEPNPEPREAAGRRENETPDVERMSQIAEEKLEAERKELLLLHKRLDEEKELLRQQKMKQEEEEMLKEKETDSQHLQHRKHHHLHTTTPKPGKRIKETQSRLLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIZ3</accession>
    <name>A0A3B5MIZ3_9TELE</name>
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        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIZ3"/>
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    <dbReference type="Ensembl" id="ENSXCOT00000023866.1">
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        <property type="gene ID" value="ENSXCOG00000017621.1"/>
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        <property type="component" value="Unplaced"/>
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    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
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    </dbReference>
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        <property type="entry name" value="IM:7136021-RELATED"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="2"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="406" mass="46485" checksum="354873D20D2B8BB0" modified="2018-12-05" version="1">MERPPPHLPAEIWTHIFGYLSVSDKLSTRACCMHFRKLIDHWSLWRGWTAVLTFKNGSYSKRFWNSLRRRKVTSVVVRSPRAKHWRQLSSELPHLSSLVLEQSVMENLGFGKDFPHLERLAIRNSDVVIDVHKRTHLEQLTHLSLCDVTFPIKSSFLPFISHLRNLTSLTCHNSGTSVEPICIMETILSYLPKLKHLSLSSKRACVYVPKRPLNEGISFLSTLELVQYSDHFFPVNTMKKVPHLNSLSVFSGNEHQRMPSLSSHLVFLLSKWLQDLPKLTKLAVTRGPPVRTYVTSIPATVTSLALCSSGLSSEDMSAVSAQLPNLLHLHIDPRPSHLGVNIAQIPELFPKLRSLKLRHERVPEEDFLHLRRLQHLEHLEILDGARSPSHLAQYIFLEIIVLKTMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJ08</accession>
    <name>A0A3B5MJ08_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="ENSXCOG00000014687.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="DENN domain-containing protein 2C isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051942">
        <property type="entry name" value="DENN_domain_containing_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15288:SF3">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03455">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00801">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="2">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="309" mass="35312" checksum="0A786779B26A5A86" modified="2018-12-05" version="1">PFMRAIMEAQFPAPGRTINIKTFLPGSGTEVMELCRPSDSRLEHVDFECLFSCLSLRLLLRVFSSLLLERRVIFTADKLSTLSQCCHAVVALLYPFVWQHTYIPVLPSAMLDIVCTPTPFIVGLLSSSLAQLNELPLEEVRLKALDDEDSILPSKLQSALENVLERRRELANDRGADSINTGHLSTVVSEAFVRFFVELVGHYPLFITAEREDGYSSSSSSSPASCAFQREGFRKAIPSKTVRRFLEVFMETQMFGWFIQERELHRQALRGLFEVRVQEYLDSINENEHRRVNRFLKGLGNRMKFLSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MK69</accession>
    <name>A0A3B5MK69_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E3 ubiquitin-protein ligase RNF182</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">RING finger protein 182</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase RNF182</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Interacts with ATP6V0C.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MK69"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024260.1">
        <property type="protein sequence ID" value="ENSXCOP00000023975.1"/>
        <property type="gene ID" value="ENSXCOG00000017903.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111020"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042285">
        <property type="entry name" value="RNF182"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46675">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46675:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="16"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="238" mass="26437" checksum="5C2F15B5D9936F61" modified="2018-12-05" version="1">MSSFSSHFYQNTELECKICYQRYNANNRKPKILDCLHRVCSRCLVKILDVTDGVGCIPCPFCRHQTEITEYQVSALPDDVNIMSHLTMREKSCSLDKKREVVLTPKSLSSTSPSHDSSNCLVITIMEVQRDLQHSPSHNGSSDIYADQSLDSVSIGSNGPTDHDALSKFCNHVPRILVWLLGFLYFGSLPLGIYLLVIQRVTLGIVCVSLVPSSLTVCLVYGFCQCLCQGMCDCSARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MMI2</accession>
    <name>A0A3B5MMI2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MMI2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024766"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025067.1">
        <property type="protein sequence ID" value="ENSXCOP00000024766.1"/>
        <property type="gene ID" value="ENSXCOG00000018498.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01060000250476"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16513">
        <property type="entry name" value="RING-HC_LONFs_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327:SF5">
        <property type="entry name" value="LON PEPTIDASE N-TERMINAL DOMAIN AND RING FINGER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327">
        <property type="entry name" value="RING FINGER PROTEIN 127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="64"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="164" mass="18201" checksum="A6B8F6E8D7ADEE49" modified="2018-12-05" version="1">MSLIEILRPEELLVLVDTIARTLRGKELGLPVTLNGQTKSDGGQDGAHNDGECEEDEALDLFSCRLCKCLLQEPATLECGHTFCKRCVDGDAVKYCISCKQKPSKGDRRVNVVLSGLLDKLFETESKARKMWIEGEVLPLTQKPGCSSQVNARTVQPSTHFRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MN20</accession>
    <name>A0A3B5MN20_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Telethonin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MN20"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022826.1">
        <property type="protein sequence ID" value="ENSXCOP00000022551.1"/>
        <property type="gene ID" value="ENSXCOG00000016859.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012014"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031432">
        <property type="term" value="F:titin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070080">
        <property type="term" value="F:titin Z domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060048">
        <property type="term" value="P:cardiac muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055008">
        <property type="term" value="P:cardiac muscle tissue morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055003">
        <property type="term" value="P:cardiac myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035995">
        <property type="term" value="P:detection of muscle stretch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048769">
        <property type="term" value="P:sarcomerogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003009">
        <property type="term" value="P:skeletal muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030241">
        <property type="term" value="P:skeletal muscle myosin thick filament assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030240">
        <property type="term" value="P:skeletal muscle thin filament assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.160.10">
        <property type="entry name" value="titin domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015667">
        <property type="entry name" value="Telethonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023111">
        <property type="entry name" value="Titin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15143">
        <property type="entry name" value="TELETHONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15143:SF0">
        <property type="entry name" value="TELETHONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09470">
        <property type="entry name" value="Telethonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="15740" checksum="6B29DAD995F820ED" modified="2018-12-05" version="1">MHCVSRGRVCLVKSYSDLQEDDLCKKESYSACWLNMVLETRPEYMTLSETDSARRESYKQQQVVHLMVERRPSQTLKLGIEGGVMREYQLPFSNRTKAPQRASDTEEPPVRENFRASRLMSSPRNVSHREQRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MN63</accession>
    <name>A0A3B5MN63_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MN63"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020899"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021156.1">
        <property type="protein sequence ID" value="ENSXCOP00000020899.1"/>
        <property type="gene ID" value="ENSXCOG00000015648.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012436"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21982">
        <property type="entry name" value="HMG-box_HMGXB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042477">
        <property type="entry name" value="HMGXB4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048016">
        <property type="entry name" value="HMGXB4_HMG-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46584">
        <property type="entry name" value="HMG DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46584:SF1">
        <property type="entry name" value="HMG DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00886">
        <property type="entry name" value="HIGHMOBLTY12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="2">
        <location>
            <begin position="3"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="1">
        <location>
            <begin position="3"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="179" mass="19464" checksum="FAFC69168801EC6B" modified="2018-12-05" version="1">MTKKKTTTAYQVFCKEYRVNINAEHPGLDFGELSKKLAEVWKAMPEKDKLVWRQKAQYLQHKQNKAEATTVKQKSFSEGKTKGTAAGLVSPTRTPGTVSLSPARVPDVDPIDAAAHLQLLGESLSLIGHRLQETEGMVAVSGSLSVLLDSILCALGPLTCLTAQIPQLNGCPRNVLVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MP88</accession>
    <name>A0A3B5MP88_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ets2 repressor factor like 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MP88"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025598"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025907.1">
        <property type="protein sequence ID" value="ENSXCOP00000025598.1"/>
        <property type="gene ID" value="ENSXCOG00000019124.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163212"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000059">
        <property type="entry name" value="ETS translocation variant 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF165">
        <property type="entry name" value="ETS DOMAIN-CONTAINING TRANSCRIPTION FACTOR ERF-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ETS" evidence="9">
        <location>
            <begin position="39"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="257"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="329"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="418"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="357"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="436"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="468" mass="50728" checksum="B2E15C976C910E49" modified="2018-12-05" version="1">MDCNCVSDLLLPPVPALWTPFAFPDWAYKPESSPGSRQIQLWHFILELLQKEEYQGVIAWQGDYGEFVIKDPDEVARLWGIRKCKPHMNYDKLSRALYYYNKRILHKTKGKRFTYKFNFSKVVLVNYPILDMANSPFFLAQNHFNGGSAAPDCSPEAIQSLFPRLPDSGRGTSLFDRGSAPPGPEGDKLRLDTFPFLGSAPCYSKPPSLLGPYPRNPPFDYPWSFNPYLPGAFSLNNCPKLPPGSLYPSQFYPNPLQSSLSQLPHPFSSLLPPGEAAGGERGAPGQTGGTNGTAGGQPARLCLPPYPGGLPIGRTDIGNGATLGERERVEANPPSTGLGLGLGAVSLGAGTGQQSPTERRGGVKQDPESDSDLEITDLSDCSSDNENEHDFGIGKESSLVSRSPLVLDGKKGSLLPPITSLPPPVSPHPLKSLMPLTPPPPSLPPSLSPSMALHDRHREAETLKMIHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPN3</accession>
    <name>A0A3B5MPN3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MPN3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025748"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026058.1">
        <property type="protein sequence ID" value="ENSXCOP00000025748.1"/>
        <property type="gene ID" value="ENSXCOG00000019245.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161022"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000162">
        <property type="entry name" value="E3 ubiquitin-protein ligase RING1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032443">
        <property type="entry name" value="RAWUL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043540">
        <property type="entry name" value="RING1/RING2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46076:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RING1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46076">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RING1 / RING 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16207">
        <property type="entry name" value="RAWUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="RAWUL" evidence="6">
        <location>
            <begin position="110"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="134"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="134"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="247" mass="27220" checksum="08A25470EF1BCC20" modified="2018-12-05" version="1">MSLNHRVKKPTQESDNTTFSGGEDNGDSRSHLSHDSAPSNTPHPRGQTPPEAGPSRKRQRASDDGSGPEADSGSPTPPLRRHKEGPASEIELVFRPHPQLVHGNDYNQTRYVKTTANATVDHLSKYLALRIALEERRTDGPTDNRGKEEGGAEGEGETGAASRSGEGTSLSNISEKQYTIYITTRGGQFSTLNGSLTLELVNEKYWKVRKPLELYYAPTKEQQQPQCPQDRLSLHHSSVHLPVFEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQF2</accession>
    <name>A0A3B5MQF2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Integrin beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="primary">ITGB1</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="26">Cell membrane</location>
            <topology evidence="26">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Invadopodium membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Ruffle membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Melanosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 26">Belongs to the integrin beta chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQF2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026644"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026968.1">
        <property type="protein sequence ID" value="ENSXCOP00000026644.1"/>
        <property type="gene ID" value="ENSXCOG00000019898.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286983"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019960">
        <property type="term" value="F:C-X3-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098639">
        <property type="term" value="F:collagen binding involved in cell-matrix adhesion"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001968">
        <property type="term" value="F:fibronectin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043236">
        <property type="term" value="F:laminin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.100:FF:000002">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000043">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000075">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000155">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000002">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040622">
        <property type="entry name" value="EGF_integrin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057073">
        <property type="entry name" value="EGF_integrin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015812">
        <property type="entry name" value="Integrin_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014836">
        <property type="entry name" value="Integrin_bsu_cyt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012896">
        <property type="entry name" value="Integrin_bsu_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036349">
        <property type="entry name" value="Integrin_bsu_tail_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002369">
        <property type="entry name" value="Integrin_bsu_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057243">
        <property type="entry name" value="Integrin_I-EGF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082:SF62">
        <property type="entry name" value="INTEGRIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082">
        <property type="entry name" value="INTEGRIN BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23105">
        <property type="entry name" value="EGF_integrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18372">
        <property type="entry name" value="I-EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08725">
        <property type="entry name" value="Integrin_b_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07965">
        <property type="entry name" value="Integrin_B_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00362">
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    </dbReference>
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        <property type="entry name" value="Integrin_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01186">
        <property type="entry name" value="INTEGRINB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00187">
        <property type="entry name" value="INB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01241">
        <property type="entry name" value="Integrin_b_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01242">
        <property type="entry name" value="Integrin_B_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69687">
        <property type="entry name" value="Integrin beta tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00243">
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    </dbReference>
    <dbReference type="PROSITE" id="PS52047">
        <property type="entry name" value="I_EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="14" id="KW-0106">Calcium</keyword>
    <keyword evidence="16 26" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="17" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="24" id="KW-0966">Cell projection</keyword>
    <keyword evidence="21 25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="23" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="19 26" id="KW-0401">Integrin</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="20 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0517">Myogenesis</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-0675">Receptor</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="10 26 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18 27" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="682"/>
            <end position="704"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="705"/>
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        </location>
    </feature>
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        <location>
            <begin position="3"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="444"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="455"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
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    <feature type="disulfide bond" evidence="25">
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    <feature type="disulfide bond" evidence="25">
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    <feature type="disulfide bond" evidence="25">
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    <feature type="disulfide bond" evidence="25">
        <location>
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    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
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    </feature>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="26">
        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SMART" id="SM01241"/>
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    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="SMART" id="SM01242"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="751" mass="83191" checksum="3B634D86D865B426" modified="2018-12-05" version="1">SSCSSLNFLTVGSPKSARCDDLEVLKERKCQQVENPRGKIIINKDTPVTARNDGPVKLKPEQITQIQPQNLTLKLRSGEPQTFSLKFKRAEDYPIDLYYLMDLSFSMRDDLENVKKLGTDLMREMKKITSDFKIGFGSFVEKTVMPYISTTPARLLNPCQGSENCTSPFSYKNVLKLTENGEEFNRLVSQQQISGNLDSPEGGFDAIMQVAVCQDQIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGKCHLENNMYTMSHYYDYPSIAHLVQKLSEHNIQTIFAVTEEFQSVYKELKNLIPKSAVGTLSADSNNVMKLIIDAYNSLSSEVVLENPKRPAGASISYKSICKDNVVNTGENGNKCVNISIGDEVRFDITVESQKCPSNGNPEVFTIKPQGFREEVTVILDYICDCDCATKGEPNSQRCSAGSGSYQCGSCKCNEGRIGQTCECSKDDVRTEDLDANCRKDNGTDICSNNGDCVCGTCECRKRDNPAEAYSGQFCECDNFNCDRSNNKLCGGHGRCECRKCICDANYTGSACDCSMDTSTCVASNGQICNGRGECECGGCKCTDSKFQGPTCEACPTCPGVCTEHKDCVECLTFQTGVKKDVCKDECKNFRLFRVQDQGQLPQPNDQTFPQVICKERDENDCSFYFSFAVRNNTNEVYVVEKPECPSSPDIIPIVAGVVAGIVMIGLALLLIWKLLMIIHDRREFAKFEKEKMNAIWDTGENPIYKSAVTTVVNPKYEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRA6</accession>
    <name>A0A3B5MRA6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">Guanine deaminase</fullName>
            <shortName evidence="12">Guanase</shortName>
            <ecNumber evidence="4 12">3.5.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Guanine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10 12">Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>guanine + H2O + H(+) = xanthine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:14665"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Purine metabolism; guanine degradation; xanthine from guanine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the metallo-dependent hydrolases superfamily. ATZ/TRZ family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.3" evidence="4 12"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRA6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022019"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022286.1">
        <property type="protein sequence ID" value="ENSXCOP00000022019.1"/>
        <property type="gene ID" value="ENSXCOG00000016453.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017130"/>
    <dbReference type="UniPathway" id="UPA00603">
        <property type="reaction ID" value="UER00660"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008892">
        <property type="term" value="F:guanine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006147">
        <property type="term" value="P:guanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000021">
        <property type="entry name" value="Guanine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014311">
        <property type="entry name" value="Guanine_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051607">
        <property type="entry name" value="Metallo-dep_hydrolases"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02967">
        <property type="entry name" value="guan_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11271">
        <property type="entry name" value="GUANINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11271:SF6">
        <property type="entry name" value="GUANINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="13">
        <location>
            <begin position="32"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="377" mass="41947" checksum="E029C619F05EB38F" modified="2018-12-05" version="1">IAFIGEGKDLQKLSENFGFSPSAVTQLQQEFFMPGMIDTHIHASQYSYAGTALDMPLLQWLNMYTFPVESRFQDVEFAKKVYTQVVKRTLRNGTTTACYFATIHTDASLLLGQIADFGQRALVGKVCMDRNDAVKCYRETSEESQEETYQYPLVKPVVTPRFAPSCTEALLAKLGEIAKNNKLHIQSHISENLEEVELVKELFPNSESYTDIYHKCNILTDKTIMAHGCHLSDEELVLFRKTGAALAHCPNSNILCSGVLNVRNVLNHKVKLGLGTDMAGGYSPSILDAMRRSLDSSKVLTIQDPEHKTLSFEEVFRLATLGGLSLDERTGNFKVGKDFDALRVNVAAPGGPIILLEKFLNLDDRNIVEVFVAGKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSN0</accession>
    <name>A0A3B5MSN0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Neuropeptide Y</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">NPY</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the NPY family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSN0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026501"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026822.1">
        <property type="protein sequence ID" value="ENSXCOP00000026501.1"/>
        <property type="gene ID" value="ENSXCOG00000019786.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156475"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031841">
        <property type="term" value="F:neuropeptide Y receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007631">
        <property type="term" value="P:feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00126">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001955">
        <property type="entry name" value="Pancreatic_hormone-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020392">
        <property type="entry name" value="Pancreatic_hormone-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10533:SF12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10533">
        <property type="entry name" value="NEUROPEPTIDE Y/PANCREATIC HORMONE/PEPTIDE YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00159">
        <property type="entry name" value="Hormone_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00278">
        <property type="entry name" value="PANCHORMONE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00309">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00265">
        <property type="entry name" value="PANCREATIC_HORMONE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50276">
        <property type="entry name" value="PANCREATIC_HORMONE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017236997" evidence="5">
        <location>
            <begin position="21"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="92" mass="10395" checksum="3EB5DA711BDC8635" modified="2018-12-05" version="1">MSPTLLALCLLACFLSGRDAYPVKPANPREGAPPEELAKYYSALRHYINLITRQRYGKRDNPDTVFSDLFVKESAETLPGSNYIRYEGLPGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MTD6</accession>
    <name>A0A3B5MTD6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027892535.1">
        <property type="nucleotide sequence ID" value="XM_028036734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MTD6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027361.1">
        <property type="protein sequence ID" value="ENSXCOP00000027028.1"/>
        <property type="gene ID" value="ENSXCOG00000020189.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114156356"/>
    <dbReference type="KEGG" id="xco:114156356"/>
    <dbReference type="GeneTree" id="ENSGT00940000162818"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002353">
        <property type="entry name" value="AntifreezeII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00356">
        <property type="entry name" value="ANTIFREEZEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="37"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="162" mass="18175" checksum="4E515FC27153E2B6" modified="2018-12-05" version="1">MILLFFLFGLSLAAVAPSDRQEMKLQRGGCPPFWFSFNGRCYKYVATLMTWADAEQHCVNQGANLVSIHSLEEENFVKMLIRNFDHAEGANWIGLSDAQKDGTYFWSDGSRFNFKLWNAGEPNNGGGSEPCVHTNYGPSKGWNDKVCTVNYSSVCKARLDSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MTH7</accession>
    <name>A0A3B5MTH7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ferritin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the ferritin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MTH7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026806"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027135.1">
        <property type="protein sequence ID" value="ENSXCOP00000026806.1"/>
        <property type="gene ID" value="ENSXCOG00000019791.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182841"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01056">
        <property type="entry name" value="Euk_Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.10:FF:000002">
        <property type="entry name" value="Ferritin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014034">
        <property type="entry name" value="Ferritin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431:SF80">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00540">
        <property type="entry name" value="FERRITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00204">
        <property type="entry name" value="FERRITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="2 8" id="KW-0409">Iron storage</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="9">
        <location>
            <begin position="9"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601519-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="179" mass="20863" checksum="BE5485F80804E282" modified="2018-12-05" version="1">IEMTSQIRQNFHQDCEAAINRQINLELYASYVYLSMAYYFDRDDKSLENFAKFFNAQSKEEREHAEKLMGLQNKRGGRIFLQDIKKPDRDEWGSGLEALECALQLEKSVNQSLLDLQKMAAEHNDPHMCDFVETHFLDEQVKSIKQLADWISNLRRMGAPQNGMAEYLFDKHTMGMEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MU03</accession>
    <name>A0A3B5MU03_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sodium/potassium-transporting ATPase subunit beta-1-interacting protein</fullName>
            <shortName evidence="9">Na(+)/K(+)-transporting ATPase subunit beta-1-interacting protein</shortName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Interacts with atp1b1 C-terminus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the NKAIN family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MU03"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026976"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027309.1">
        <property type="protein sequence ID" value="ENSXCOP00000026976.1"/>
        <property type="gene ID" value="ENSXCOG00000020149.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159949"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002028">
        <property type="term" value="P:regulation of sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008516">
        <property type="entry name" value="Na/K-Atpase_Interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13084:SF4">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT BETA-1-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13084">
        <property type="entry name" value="T-CELL LYMPHOMA BREAKPOINT-ASSOCIATED TARGET 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05640">
        <property type="entry name" value="NKAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="205" mass="23591" checksum="F4E7C3E8BEBC4B1F" modified="2018-12-05" version="1">MGKCDGRCTLLVICSLQLVAALQRQVFDFLGYQWAPILANFLHIMAVILGMFGTVQFRFRYLIFYAVWLVLWVGWNSFIICFYLEVGNLSQDRDFLMTFNTSLHRSWWMEHGPGCLVTPVLDSRMAPDDHHVITVSGCLLDYQYIEVLSSAIQILLALFGFVYACYVSKVFQDDEDSFDFIGGFDSYGYQPPQKSSHLQLQPLYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUQ5</accession>
    <name>A0A3B5MUQ5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquinol-cytochrome c reductase complex assembly factor 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBP3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MUQ5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023224"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023502.1">
        <property type="protein sequence ID" value="ENSXCOP00000023224.1"/>
        <property type="gene ID" value="ENSXCOG00000017352.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018118"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021150">
        <property type="entry name" value="Ubiq_cyt_c_chap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007129">
        <property type="entry name" value="Ubiqinol_cyt_c_chaperone_CPB3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12184">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX ASSEMBLY FACTOR 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12184:SF1">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME-C REDUCTASE COMPLEX ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03981">
        <property type="entry name" value="Ubiq_cyt_C_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquinol-cytochrome c chaperone" evidence="2">
        <location>
            <begin position="72"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="234" mass="27041" checksum="78C4A69732E3B597" modified="2025-10-08" version="2">MTLLGQVLPNQKVCSQVATEKQPGPPRPSHRYLLQSCTGGRCRSPPGKIKIAALRMYTCCVERINYDEFFDKCFLPDTLNSWFLIAQLHVWMCLVRMRQEGREGKFMCRYIVHSMWEDVEQRSKIMGVSRVWKEAMKAMTETFYAAIFGYDEGILSDDCVLAAALWRNLFNCQCDDPQQLELMVEYVRKQMQFIDSLDGEDLLLTGEVKWRPLVEENAQSILKVVSPTYNDTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUZ2</accession>
    <name>A0A3B5MUZ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribonuclease H2 subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Ribonuclease HI subunit B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The RNase H2 complex is a heterotrimer composed of the catalytic subunit RNASEH2A and the non-catalytic subunits RNASEH2B and RNASEH2C.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNase H2 subunit B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MUZ2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025092"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025397.1">
        <property type="protein sequence ID" value="ENSXCOP00000025092.1"/>
        <property type="gene ID" value="ENSXCOG00000018732.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011439"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09270">
        <property type="entry name" value="RNase_H2-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.120:FF:000002">
        <property type="entry name" value="Ribonuclease H2 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040456">
        <property type="entry name" value="RNase_H2_suB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019024">
        <property type="entry name" value="RNase_H2_suB_wHTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041195">
        <property type="entry name" value="Rnh202_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13383">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13383:SF11">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09468">
        <property type="entry name" value="RNase_H2-Ydr279"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17745">
        <property type="entry name" value="Ydr279_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rnh202 triple barrel" evidence="10">
        <location>
            <begin position="30"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Ribonuclease H2 subunit B wHTH" evidence="9">
        <location>
            <begin position="89"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="229"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="299" mass="33973" checksum="7D987F644C5462DD" modified="2018-12-05" version="1">MAAKKKRTPNTHNDSWVVIAASAVETQKHDCDPDFVKLRNPSTLYMLGAGDVQLYEVKAFEEDFHSWFVGQTVQRDGKLLFVTQMDPLYLILPYMIKSGQEQGKFQPVDQVVKDDEFPASSRLLSCTRSLTSLHHIAEGKVGSLKFHRYSQERTLNWLQKKVERTIVALKKKHICVGEGVKSTTYVRVKTESDNQEDYLRYAHGLISEYISEELSKALLKHLLPELKSAKETEPPSKKRKLSDKPVEAGEDYTKFNSGDFARKPPKKMTAAQKTLAKVDKSGMKPMSSFFSPKTKAEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MV44</accession>
    <name>A0A3B5MV44_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Polypeptide N-acetylgalactosaminyltransferase 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GALNT10</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MV44"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025157"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025462.1">
        <property type="protein sequence ID" value="ENSXCOP00000025157.1"/>
        <property type="gene ID" value="ENSXCOG00000018793.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156690"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000248">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF41">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="11">
        <location>
            <begin position="5"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="262" mass="30470" checksum="C89D1AA23C257A9E" modified="2018-12-05" version="1">MTRVPKVHILRTKKREGLIRTRLLGATAAKGEVITFLDSHCEANVNWLPPLLRIAQNRKTIVCPMIDVIDHDHFGYDTQAGDAMRGAFDWEMYYKRIPIPPEMKRDDPTEPFRSPVMAGGLFAVDRKWFWELGGYDTGLEIWGGEQYEISFKVWMCGGRMEDIPCSRVGHIYRKYVPYKIPGGISLAKNLKRVAEVWMDEYAEYVYQHRPEYRHLSAGDMTAQKELRSRLSCKNFRWFMSEVAWDLPRHYPPVEPPAAAWGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVE8</accession>
    <name>A0A3B5MVE8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">G protein-coupled receptor 161b</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVE8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025282"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025588.1">
        <property type="protein sequence ID" value="ENSXCOP00000025282.1"/>
        <property type="gene ID" value="ENSXCOG00000018883.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157829"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:recycling endosome"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007368">
        <property type="term" value="P:determination of left/right symmetry"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051924">
        <property type="term" value="P:regulation of calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
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        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 161"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
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    <keyword evidence="6 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 14" id="KW-0675">Receptor</keyword>
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    <keyword evidence="12 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
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        <location>
            <begin position="36"/>
            <end position="315"/>
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        <location>
            <begin position="225"/>
            <end position="247"/>
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    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="481"/>
            <end position="515"/>
        </location>
    </feature>
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        <location>
            <begin position="235"/>
            <end position="247"/>
        </location>
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        <location>
            <begin position="406"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="493"/>
            <end position="504"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004272"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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            <dbReference type="ARBA" id="ARBA00023040"/>
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            <dbReference type="ARBA" id="ARBA00023069"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="PROSITE" id="PS50262"/>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025282.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="515" mass="56463" checksum="A1A898213FEC3F60" modified="2018-12-05" version="1">LTMNASRNCTSVGNGDGLAVLESVSILTITLLACLGNLLIVATLYRRPYLLTPSNKFVFSLTLSNLLLSVLVLPFVAVSSAKREWVFGVVWCNFTALLYLLISSASMLTLGAIAIDRYYAVLYPMIYPMKITGNRAVVVIVYVWLHSLVGCLPPLFGWSSFEFDCLKRTCVAAWSREPSYAAFWVTWCILPPFFIMLACYGVIFRVARMKARKIHCGTVIVAQDDSSGTQRNGRKNSSTSTSSNGSRRSLVYAGSQCKAFVTILVVIGTFLITWGPYVGVVCTEALWGQGNVSQELETLVAWLSFCSAVCHPLIYGLWNKTVRKELLGMCFGDRYYRESFATRQRTSRLFSISNRITDLGMSPHLTAMLAGGGHLLAQGSSTGDTGFSFTQDSGTDVMLLENSCTDGSSFPSQNGNPSGKRRSSVTFEDQVEHSKAENTSSAQVHAETHKSLDSFASCLAKAIESDAKLTLFEQSLAPQGGGLFVTRGAQKPRYPDGQRLRLESIDEAAEQTQRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVG7</accession>
    <name>A0A3B5MVG7_9TELE</name>
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        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tropomyosin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVG7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027818"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028153.1">
        <property type="protein sequence ID" value="ENSXCOP00000027818.1"/>
        <property type="gene ID" value="ENSXCOG00000020742.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="F:actin binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="8"/>
            <end position="91"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00009036"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027818.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="129" mass="14980" checksum="AA3F7B3599BDEAA6" modified="2018-12-05" version="1">MSGGMNSVDAVKKKIKVLQDQAEEAEERAEQLQLEVEKEKRTREEAEGEVTSLSRRLQLSEENLDRVQERLAAALRKLDEVEKVADESERSPVFRYLKQNKNQYLSILTGMKRLYQLYRLALPLADRNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWV0</accession>
    <name>A0A3B5MWV0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hsp70-binding protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Heat shock protein-binding protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Hsp70-interacting protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Interacts with the ATP-binding domain of HSPA1A. Detected in a ternary complex containing STUB1, HSPA1A and HSPBP1. Interacts with PGLYRP1; this interaction blocks the cytotoxic activity of the PGLYRP1-HSPA1A complex.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027898141.1">
        <property type="nucleotide sequence ID" value="XM_028042340.1"/>
    </dbReference>
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    <dbReference type="STRING" id="32473.ENSXCOP00000024274"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024565.1">
        <property type="protein sequence ID" value="ENSXCOP00000024274.1"/>
        <property type="gene ID" value="ENSXCOG00000018124.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114160006"/>
    <dbReference type="KEGG" id="xco:114160006"/>
    <dbReference type="CTD" id="23640"/>
    <dbReference type="GeneTree" id="ENSGT00940000153909"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000178">
        <property type="entry name" value="hsp70-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050693">
        <property type="entry name" value="Hsp70_NEF-Inhibitors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013918">
        <property type="entry name" value="Nucleotide_exch_fac_Fes1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316:SF18">
        <property type="entry name" value="HSP70-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19316">
        <property type="entry name" value="PROTEIN FOLDING REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08609">
        <property type="entry name" value="Fes1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nucleotide exchange factor Fes1" evidence="8">
        <location>
            <begin position="14"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="53"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00064806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00069271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00075420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00081319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="329" mass="37248" checksum="1B1F0910B2117CC5" modified="2018-12-05" version="1">MSDNRQDRRYPQNLQGVLQLAVEAGSAAEGPVPSEPMSEERKTWLREALSDICKGQMDEVEQMKQSLAVLQKEQDGEEEREEDEEDERELAFEMLSELCENLDNARDLMTLGGLELCVSRYLCHVQSGLRWRAAQLLASCAQNMPQVQEYLLKADVLPKLLQLTDSDPNPTVRVKALYAVSCLVREQEAGLQAFVSHDGFSVLMRGMQSENEKVRTKSAFLLLNLLTSNPEHKETVVSMGMVQQLVSVLRTPHSPFHEHVLGALCCLVEDFQQGLEDCRSPALGLEEFLKQRTKELQGKEESQEELDFCERLRVLCFNGQQTDDVGMDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXJ2</accession>
    <name>A0A3B5MXJ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flavodoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXJ2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026382"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026702.1">
        <property type="protein sequence ID" value="ENSXCOP00000026382.1"/>
        <property type="gene ID" value="ENSXCOG00000019701.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000178129"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017460190" description="Flavodoxin-like domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Flavodoxin-like" evidence="3">
        <location>
            <begin position="86"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="143" mass="15890" checksum="316BA91276231323" modified="2018-12-05" version="1">MLALFTVISFLAGSLSTSDTKVPQDVRSPTENYLLWTWNNRLYVYSAAALLLGFCFLLQVTRRKVQKAAKNQISSDKDGDVHVSGVKIFYGSQTGTAKGFASELSQEVQTLGIPAEVIDMKDYDPDDRLADEVIWWKSLPLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXL3</accession>
    <name>A0A3B5MXL3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXL3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024770"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025071.1">
        <property type="protein sequence ID" value="ENSXCOP00000024770.1"/>
        <property type="gene ID" value="ENSXCOG00000018500.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157684"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900181">
        <property type="term" value="P:negative regulation of protein localization to nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046620">
        <property type="term" value="P:regulation of organ growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000391">
        <property type="entry name" value="Large tumor suppressor kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000024">
        <property type="entry name" value="Probable serine/threonine-protein kinase cot-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF138">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE LATS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="25"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="188" mass="21873" checksum="8CC97963D96774B5" modified="2018-12-05" version="1">MRMMLSQKESNYIRLKRAKMEKSMFKRIKTLGIGAFGEVSLARKEDTGALYAMKTLRKKDVLLRNQVAHVKAERDILAEADNEWVVRLYYSFQDKDNLYFVMEYIPGGDMMSLLIRLGIFKEELAQFYIAELTCALESVHKMGFIHRDIKPDNILIDRDGHIKLTDFGLLKRILKNCLKISSVCCLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZP2</accession>
    <name>A0A3B5MZP2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 17">NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial</fullName>
            <ecNumber evidence="4 17">7.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:29092"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="15">Core subunit of respiratory chain NADH dehydrogenase (Complex I) which is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme. Interacts with RAB5IF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Mitochondrion inner membrane</location>
            <topology evidence="17">Peripheral membrane protein</topology>
            <orientation evidence="17">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.2" evidence="4 17"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MZP2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026090"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026404.1">
        <property type="protein sequence ID" value="ENSXCOP00000026090.1"/>
        <property type="gene ID" value="ENSXCOG00000019489.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010641"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.230:FF:000001">
        <property type="entry name" value="Mitochondrial NADH dehydrogenase flavoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.600:FF:000001">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11540:FF:000001">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.230">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050837">
        <property type="entry name" value="ComplexI_51kDa_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001949">
        <property type="entry name" value="NADH-UbQ_OxRdtase_51kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011537">
        <property type="entry name" value="NADH-UbQ_OxRdtase_suF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037207">
        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054765">
        <property type="entry name" value="SLBB_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01959">
        <property type="entry name" value="nuoF_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010120">
        <property type="entry name" value="PRK13596.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE FLAVOPROTEIN 1 NDUFV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22461">
        <property type="entry name" value="SLBB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="Nqo1 FMN-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142984">
        <property type="entry name" value="Nqo1 middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="Nqo1C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00644">
        <property type="entry name" value="COMPLEX1_51K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00645">
        <property type="entry name" value="COMPLEX1_51K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="17" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 17" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 17" id="KW-0288">FMN</keyword>
    <keyword evidence="11 17" id="KW-0408">Iron</keyword>
    <keyword evidence="12 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13 17" id="KW-0520">NAD</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="17" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="17" id="KW-0813">Transport</keyword>
    <keyword evidence="14" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="NADH-ubiquinone oxidoreductase 51kDa subunit iron-sulphur binding" evidence="18">
        <location>
            <begin position="340"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU364066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="443" mass="49115" checksum="0732378F3DCCD304" modified="2018-12-05" version="1">MKPKKAKFGPLSDQDRIFTNLYGRHDWRLKGALKRGDWYKTKEIVLKGPEWILNEVKISGLRGRGGAGFPTGMKWSFMNKPSDGRPKYLVVNADEGEPGTCKDREILRHDPHKLIEGCLIAGRAMGARAAYIYIRGEFYNESSNLQVAINEAYKARLIGKNACGSGYNFDVFVMRGAGAYICGEETALIESLEGKQGKPRLKPPFPADIGVFGCPTTVANVETVAVAPAICRRGGAWFASFGRERNSGTKLFNISGHVNFPCTVEEEMSIPLRELIERHAGGVRGGWGNLLGVIPGGSSTPIIPQHVCSDVLMDFDDLVRAQTALGTAAVIVMDKSTDVIRAIARLVEFYKHESCGQCTPCREGVDWMDKMMWRFVRGDAAVSEIDMIWELSKQIEGHTICALGDGAAWPVQGLIRHFRTDMENRIAQHKEKNPPRKQKIEMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N1X0</accession>
    <name>A0A3B5N1X0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thyroglobulin type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N1X0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028196.1">
        <property type="protein sequence ID" value="ENSXCOP00000027860.1"/>
        <property type="gene ID" value="ENSXCOG00000020777.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177230"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00191">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.10">
        <property type="entry name" value="Thyroglobulin type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051950">
        <property type="entry name" value="Dev_reg/Prot_inhib"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036857">
        <property type="entry name" value="Thyroglobulin_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12352:SF3">
        <property type="entry name" value="NIDOGEN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12352">
        <property type="entry name" value="SECRETED MODULAR CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
        <property type="entry name" value="Thyroglobulin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00211">
        <property type="entry name" value="TY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
        <property type="entry name" value="Thyroglobulin type-1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00484">
        <property type="entry name" value="THYROGLOBULIN_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Thyroglobulin type-1" evidence="6">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="78" mass="8572" checksum="682B43442FB152E8" modified="2018-12-05" version="1">MNFEERRAALDSSSVFVPSCEAGGRFSPKQCQQGALCWCVDPTGRQLTGPRRPGDGLVCGEDEPRRFGRLLLSVQTEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KJF3</accession>
    <name>A0A3B5KJF3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Calcitonin peptide-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the calcitonin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KJF3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000268"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000275.1">
        <property type="protein sequence ID" value="ENSXCOP00000000268.1"/>
        <property type="gene ID" value="ENSXCOG00000000255.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156267"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031716">
        <property type="term" value="F:calcitonin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021118">
        <property type="entry name" value="Calcitonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021117">
        <property type="entry name" value="Calcitonin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021116">
        <property type="entry name" value="Calcitonin/adrenomedullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018360">
        <property type="entry name" value="Calcitonin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001693">
        <property type="entry name" value="Calcitonin_peptide-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10505:SF16">
        <property type="entry name" value="CALCITONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10505">
        <property type="entry name" value="CALCITONIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00214">
        <property type="entry name" value="Calc_CGRP_IAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00270">
        <property type="entry name" value="CALCITONINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00113">
        <property type="entry name" value="CALCITONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00258">
        <property type="entry name" value="CALCITONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0027">Amidation</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0372">Hormone</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Calcitonin peptide-like" evidence="12">
        <location>
            <begin position="16"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="41"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="18"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR621116-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="71" mass="7851" checksum="CB07997FF38B74F4" modified="2018-12-05" version="1">MLPCRYISAMDRPLTKRCSNLSTCVLGKLSQELHKLQTFPRTNVGAGTPGKKRGAPERDSYASYGETFDSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KKL0</accession>
    <name>A0A3B5KKL0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KKL0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000713"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000723.1">
        <property type="protein sequence ID" value="ENSXCOP00000000713.1"/>
        <property type="gene ID" value="ENSXCOG00000000601.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155924"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000284">
        <property type="entry name" value="probable ATP-dependent RNA helicase YTHDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF119">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="61"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="251" mass="28037" checksum="5566ACE09515576B" modified="2018-12-05" version="1">AAAPHFSSDPSLSSCRSESARLADPPPPSRPDPNVYPPPAVLQILSARAQLPVQQFEEEIMATVEQNPVVIIRGATGCGKTTQVPQYILDRFIKGGRASDCNIVVTQPRRISAVSVAERVAYERGEDLGKSCGYSVRFESVLPRPHASVLFCTVGTQSRQQGEPCCRSHHVIVDEIHERDINTDFLLVVLRDVVQAFPDVRIILMSATIDTTMFREYFFNCPIIEVFGRTFPVQDYTVKATRTNHIEKKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KMW3</accession>
    <name>A0A3B5KMW3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calponin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KMW3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001438"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001461.1">
        <property type="protein sequence ID" value="ENSXCOP00000001438.1"/>
        <property type="gene ID" value="ENSXCOG00000001165.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154539"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001997">
        <property type="entry name" value="Calponin/LIMCH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000557">
        <property type="entry name" value="Calponin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF25">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00402">
        <property type="entry name" value="Calponin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00889">
        <property type="entry name" value="CALPONIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51122">
        <property type="entry name" value="CALPONIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="84" mass="9315" checksum="01BAB7F87AEC1C6D" modified="2018-12-05" version="1">MGTNKCASQAGMTAYGTRRHLYDPKTHTDKPYDQTTISLQMGTNKGASQNASGPLALQNRTVPLQIIERKFAKNRNRKYSGLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KP12</accession>
    <name>A0A3B5KP12_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KP12"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001901.1">
        <property type="protein sequence ID" value="ENSXCOP00000001873.1"/>
        <property type="gene ID" value="ENSXCOG00000001523.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183297"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="88" mass="10071" checksum="2D01C0157C5DB971" modified="2018-12-05" version="1">MAQQGGTGVAHQDTLAVPPMPKHSGLNEDLVKILRENLLQPERPRGHRRSPSSISPRLSPRNSPRLLRRMLLNNNIHKQRRFTVAHTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KPW7</accession>
    <name>A0A3B5KPW7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Bleomycin hydrolase</fullName>
            <ecNumber evidence="3 9">3.4.22.40</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.</text>
            <dbReference type="EC" id="3.4.22.40"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.40" evidence="3 9"/>
    <dbReference type="RefSeq" id="XP_027854951.1">
        <property type="nucleotide sequence ID" value="XM_027999150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KPW7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002178"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002209.1">
        <property type="protein sequence ID" value="ENSXCOP00000002178.1"/>
        <property type="gene ID" value="ENSXCOG00000001739.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114133331"/>
    <dbReference type="KEGG" id="xco:114133331"/>
    <dbReference type="CTD" id="642"/>
    <dbReference type="GeneTree" id="ENSGT00390000001735"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070005">
        <property type="term" value="F:cysteine-type aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043418">
        <property type="term" value="P:homocysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00585">
        <property type="entry name" value="Peptidase_C1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000021">
        <property type="entry name" value="Bleomycin hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004134">
        <property type="entry name" value="Peptidase_C1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363:SF2">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03051">
        <property type="entry name" value="Peptidase_C1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005700">
        <property type="entry name" value="PepC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0788">Thiol protease</keyword>
    <feature type="active site" evidence="10">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005700-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="453" mass="52282" checksum="57227AE0C25789E6" modified="2018-12-05" version="1">MFSKGLSPEKVTTFTKRLRSEPRYLLAQNVSTCIDPLEVCLHRQTVQDTVHIFQHSIPTEGKPITNQKNSGRCWIFSCLNVLRLPFMKKHNLEEFEFSQSYLFFWDKIERCYYFLHACVETAQRKEPVDGRLVQFLLSNPTNDGGQWDMLVNLIEKYGVIPKKCFPESHSSEASRRMNDILNHKLREYCLRLRNMVASDATKAELSKAIDSMIEEVFRVASVCLGSPPDTICWEYRDKDKNFHRMGPHTPQQFYREHVKPLYNIQDKVCLVNDPRPQNPYGKLYSVEFLGNMVGGRRTLYNNQPIQLLKKAAADSIKEGEAVWFGCDVGKHFHSKLGINDMNVFNHELVFGVSVKNLSKAERLIYGDSLMTHAMILTAVTDKGGKEGYEKWRVENSWGDDRGNKGYLIMTDDWFSEYVYEVVVDKRFLSSDVLEVMQQEAVVLPAWDPMGSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KPX9</accession>
    <name>A0A3B5KPX9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kelch-like family member 24a</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027876626.1">
        <property type="nucleotide sequence ID" value="XM_028020825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KPX9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002193"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002224.1">
        <property type="protein sequence ID" value="ENSXCOP00000002193.1"/>
        <property type="gene ID" value="ENSXCOG00000001746.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114146595"/>
    <dbReference type="KEGG" id="xco:114146595"/>
    <dbReference type="CTD" id="768198"/>
    <dbReference type="GeneTree" id="ENSGT00940000154345"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd18463">
        <property type="entry name" value="BACK_KLHL24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18253">
        <property type="entry name" value="BTB_POZ_KLHL24_KRIP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030596">
        <property type="entry name" value="BTB_POZ_KLHL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047071">
        <property type="entry name" value="KLHL24_BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF215">
        <property type="entry name" value="KELCH-LIKE PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="63"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="596" mass="67206" checksum="0E50622D1A0B7529" modified="2018-12-05" version="1">MVLVLGRRINSSISDTKESPLVKRKVLPVECKVGDVFDFCSGASHSEGILQVFNEFRDCRLFTDVVISVEGREFPCHRAVLCACSSYFKAMFCNDHRESREMLVEINGIVAEAMETFLNYVYTGRAKITTENVQFLFETANLFQIATLRDACAKFLEDQLDPCNCIGIQRFADTHSLKQLASRCKSYALANFSEVSHHEEFLDLRVEELKDYIGKEDLSVSKEEIVFEAVMKWVYHNVDQRRSMLKSLLQHVRLPLLHPNYFVQTVEGDELIQNAPECYQLLHEARRYHVLGNEMMSPRTRPRRSTGYSEVIVVVGGCERVGGFNLPYNDCYDPVTGEWKSLAKLPEFTKSEYAVCTLRNDILVSGGRINSRDVWLYNSQLNLWIKVASLVKGRWRHKMGVLLGKVYAVGGYDGQSRLSSVECYDSFSNRWTEVAPMKEAVSSPAVASCSGKLYVIGGGPDDETCSDKVQCFDPNTNTWLLRASIPIAKRCITAVSLNNLIYVCGGLTKNLYCYDPTQDYWMLVCHTFTKQESCGMSVVNGKIYILGGQGENGEATDTIVCYDPATGLMSGDHGMPRPICYHGCVTIHRYCDKTKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KSK0</accession>
    <name>A0A3B5KSK0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027895130.1">
        <property type="nucleotide sequence ID" value="XM_028039329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KSK0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003078"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003115.1">
        <property type="protein sequence ID" value="ENSXCOP00000003078.1"/>
        <property type="gene ID" value="ENSXCOG00000002442.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114158022"/>
    <dbReference type="GeneTree" id="ENSGT00540000073610"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005127">
        <property type="term" value="F:ciliary neurotrophic factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070120">
        <property type="term" value="P:ciliary neurotrophic factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000151">
        <property type="entry name" value="Ciliary_neurotrophic_fac_CNTF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15196">
        <property type="entry name" value="CILIARY NEUROTROPHIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15196:SF1">
        <property type="entry name" value="CILIARY NEUROTROPHIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="191" mass="21575" checksum="9377AE0129D538D7" modified="2018-12-05" version="1">MAEQEKQPLTASDSTVSGSTQTGRAVALARLLHQECIQLLELFKERETSLSDQTPDGGRMVSLSPEATESTTEEQVQLLHSALRQCLGMIHCVIQKEEEEWGRLEDNYETLKASVRARLENLLHTTKNLLRSKDKILDVTPDHQCNEDVDGIGGTFGLKMWTYRVLLELIHWADQAAKTLHVLHEEREGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KT78</accession>
    <name>A0A3B5KT78_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Antp homeobox family. Proboscipedia subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KT78"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001697"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001722.1">
        <property type="protein sequence ID" value="ENSXCOP00000001697.1"/>
        <property type="gene ID" value="ENSXCOG00000001368.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155029"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000145">
        <property type="entry name" value="homeobox protein Hox-A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664:SF3">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664">
        <property type="entry name" value="PROTEIN ZERKNUELLT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="143"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="145"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="30"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="197"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="274"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="31"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="370" mass="40412" checksum="2CB3F42C0405D271" modified="2018-12-05" version="1">MNYEFERESGFINSQPSLAECLTSFPPVADSFQSSSIKSSTLSRPTLIPPPFEQTIPSLNPGSHPRHGRPRHSPDGCSPLPTASLPPEYPWMREKKASKKNHQVSNSTTAAAAAATTISNGPNLLAVYFSGSPEIVEGAAGGGGSRRLRTAYTNTQLLELEKEFHFNKYLCRPRRVEIAALLDLTERQVKVWFQNRRMKHKRQTQTKENHGGGDAKGPGADDGIHSEEDEEGSGLLYEQSGPLLERDTCSFQNNTQQQPQQQQQLHNNGEAASYDKNLKHFPNPSPTVPGCMSTMGPGSASGPDNGDSPSALDVSLHDFQPFSSDSCLQLSDTASPSLSESLDSPVDISADTFYFFSESLTTIDLQHLNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTP1</accession>
    <name>A0A3B5KTP1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cyclin-dependent kinase 2-associated protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CDK2AP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027874062.1">
        <property type="nucleotide sequence ID" value="XM_028018261.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027874063.1">
        <property type="nucleotide sequence ID" value="XM_028018262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTP1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000981"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000993.1">
        <property type="protein sequence ID" value="ENSXCOP00000000981.1"/>
        <property type="gene ID" value="ENSXCOG00000000818.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114144977"/>
    <dbReference type="KEGG" id="xco:114144977"/>
    <dbReference type="CTD" id="10263"/>
    <dbReference type="GeneTree" id="ENSGT00940000160334"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000134">
        <property type="term" value="P:negative regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017266">
        <property type="entry name" value="DOC_1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22607:SF4">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 2-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22607">
        <property type="entry name" value="DELETED IN ORAL CANCER 1/CDK2-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09806">
        <property type="entry name" value="CDK2AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037709">
        <property type="entry name" value="CDK2-associated_p2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="111" mass="11938" checksum="5553D8EEF50801EA" modified="2018-12-05" version="1">MSYKPIAPAPSGSNHTPPGSSVPSPSLPSSSNFRPAFSDFGPPSMGFVQPVKVSQGSTYSELLSVIEEMSREIRPTYAGSKSAMERLKRGIIHARALVRECLAETERSART</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KU84</accession>
    <name>A0A3B5KU84_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">[histone H4]-N-methyl-L-lysine20 N-methyltransferase KMT5B</fullName>
            <ecNumber evidence="3">2.1.1.361</ecNumber>
            <ecNumber evidence="4">2.1.1.362</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">[histone H4]-lysine20 N-methyltransferase KMT5B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(6),N(6)-dimethyl-L-lysyl(20)-[histone H4] + S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(20)-[histone H4] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61992"/>
            <dbReference type="Rhea" id="RHEA-COMP:15556"/>
            <dbReference type="Rhea" id="RHEA-COMP:15998"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:61993"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N(6)-methyl-L-lysyl(20)-[histone H4] + S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl(20)-[histone H4] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60348"/>
            <dbReference type="Rhea" id="RHEA-COMP:15555"/>
            <dbReference type="Rhea" id="RHEA-COMP:15556"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
            <dbReference type="EC" id="2.1.1.362"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:60349"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.361" evidence="3"/>
    <dbReference type="EC" id="2.1.1.362" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KU84"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001176"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001192.1">
        <property type="protein sequence ID" value="ENSXCOP00000001176.1"/>
        <property type="gene ID" value="ENSXCOG00000000960.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156431"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140944">
        <property type="term" value="F:histone H4K20 monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140941">
        <property type="term" value="F:histone H4K20me methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1700:FF:000001">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000006">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1700">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041938">
        <property type="entry name" value="Hist-Lys_N-MTase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039977">
        <property type="entry name" value="Suv4-20/Set9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025790">
        <property type="entry name" value="Suv4-20_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12977:SF4">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE KMT5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12977">
        <property type="entry name" value="SUPPRESSOR OF VARIEGATION 4-20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51570">
        <property type="entry name" value="SAM_MT43_SUVAR420_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="19">
        <location>
            <begin position="171"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="13"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="339"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="382" mass="43638" checksum="EC51BF97F92560B1" modified="2018-12-05" version="1">MKWLGESKNMVLNGRRHGNKLTSSDQPVSQTRSQQPQRTSLRHSRSHPRSRRCSRCNGASLEAERRYVPSSGMTAKELCENDDLTTSLILDPYLGFQTHKMNTRFRPIKGRQEELKEIIERFKKHDNMERAYKALTTGDWCRNLFLHKSKAQEKLFKQHVFVYLRIFTADSGFEILPCNRYSSEQNGAKIVATKEWKRNDKIEFLVGCIAELSESEENMLLRHGENDFSVMYSTRKNCAQLWLGPAAFINHGRLLAAPDWLPDTACVKVLRDIEPGEEISCYYGDGFFGESNEFCECYTCERRGTGAFKSKAGLPAEVPVINSKYGLRETDKRLNRLKKLGEGNRSSDSHSVGSHTDVDSQEMPKTHQCKAATHHGSIISQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KU89</accession>
    <name>A0A3B5KU89_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <property type="component" value="Unplaced"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="418" mass="45478" checksum="1D2ACB1D8600B917" modified="2018-12-05" version="1">MRQTVVRVTQNLAFNNRSVLCLKIFPLSSPGTAPTRGFPGLRNQTTYTGRPGIHGDNTTGAPGLHTTKSEDGFPGVASPAVTPRSQPLSSGYCDCLILLCRLNYLLDCIMSPWTAWSGCSVTCGLGSLFRRACFTRACPVHGHWSAWTEWSECDALCGGGVRQRSRTCSAPPPKNGGRECEGMTRQSQTCNIQPLHGGWSLWSRWSPCSSDCDSGVQTRERLCSSPTPQHRGSNCSGPHIQTKDCNSHPCSGWFIPYLDCGWSSWTQWSACTRTCDVGIRRRYRSGTDPPPAFGGRPCKGERVGVDTCSVEPCLGIREPWSSWSECSVTCGEGYRTRTRGPIRTHGTAQQFSAFDGAWSRWSDWTDCSKSCGGGIQSRRRLCDSPSPEGAGNYCEGLGTEVRACNTDHCPGSLEDLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KV69</accession>
    <name>A0A3B5KV69_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KV69"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001900"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001928.1">
        <property type="protein sequence ID" value="ENSXCOP00000001900.1"/>
        <property type="gene ID" value="ENSXCOG00000001540.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050467">
        <property type="entry name" value="LRFN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842:SF12">
        <property type="entry name" value="KEKKON 5, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842">
        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="28"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="159" mass="17109" checksum="7C43C1ED9B913DB0" modified="2018-12-05" version="1">VSAIWLNTAGRDFDVLQLRLAVQESAFPYTGELSGPPVTGTIKDVVTLPCRISGSPEPLASWILPDGNVVWQGLAVSGGLKVYSNGSLSLLTSTLKDTGYYRCIAVNQYGKETAQELLLLSILVEKSQLFCSLSLSPPSLKPTLSLHHPLLLLLTMRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KW43</accession>
    <name>A0A3B5KW43_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WAPL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WAPL family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KW43"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004338"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004388.1">
        <property type="protein sequence ID" value="ENSXCOP00000004338.1"/>
        <property type="gene ID" value="ENSXCOG00000003421.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015768"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039874">
        <property type="entry name" value="WAPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022771">
        <property type="entry name" value="WAPL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012502">
        <property type="entry name" value="WAPL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22100">
        <property type="entry name" value="WINGS APART-LIKE PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22100:SF13">
        <property type="entry name" value="WINGS APART-LIKE PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07814">
        <property type="entry name" value="WAPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51271">
        <property type="entry name" value="WAPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WAPL" evidence="2">
        <location>
            <begin position="30"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="293" mass="32530" checksum="E153EEE56380B83D" modified="2018-12-05" version="1">FNLFHYSAIALGLLPASQNKGANQTCVSGVSVHNPENQGYLIAYKDSSLIVSSAKALRRCEQMIQRYSREVKSDGGTVGKAVENCMRAIIGVLLNLTHDNEWGSTKTGEQEDLIATALNCVLKVPQYLPQEQRFDVRVLLFLQRERAAMLAEAQTDDLIKEAPKPALDQSGEWQETGGEIQWVTNDNASDKQEAKKKNNDEEDEELDLNKALQHAGKHMEDSIVASYTAQLLGCLCQGSPMNVTTVRENLPKGDFSIMTEMLKKFLNFMNLTCDVGTAGQKSISRIIDYLEHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KX50</accession>
    <name>A0A3B5KX50_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KX50"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002635.1">
        <property type="protein sequence ID" value="ENSXCOP00000002600.1"/>
        <property type="gene ID" value="ENSXCOG00000002085.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000174828"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029193">
        <property type="entry name" value="TEX12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37349">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37349:SF1">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="86" mass="9575" checksum="26AFBF95BDFEBA50" modified="2018-12-05" version="1">MCAVTNVYLHIKLVNRVSFTIFITLCLVSLPAVSLVCSSERGAADTTQMKELEGILTEARNLESYLKEKKCNLRQTLALISNKLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXZ7</accession>
    <name>A0A3B5KXZ7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXZ7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002540.1">
        <property type="protein sequence ID" value="ENSXCOP00000002506.1"/>
        <property type="gene ID" value="ENSXCOG00000002001.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000267317"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34488">
        <property type="entry name" value="SI:CH211-245H14.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34488:SF1">
        <property type="entry name" value="SI:CH211-245H14.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="150" mass="17149" checksum="AE100973F3B51EB3" modified="2018-12-05" version="1">MKRYIDLTLKRLKKRGAKLQRSPDQSDYTIVFCPIVSRFETDIQAALSTVPGSRNVILVAMHHTYDPNYTLPDKRSCDNTAVKLLVEFLFFEKKGLLKCPRNSKAKKAIWKEVISKTAPLWRYQHATYVLLLACCCQIHSTLLFSSPVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYD1</accession>
    <name>A0A3B5KYD1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="3.1.3.48" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KYD1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000559"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000568.1">
        <property type="protein sequence ID" value="ENSXCOP00000000559.1"/>
        <property type="gene ID" value="ENSXCOG00000000491.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000176289"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF449">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="3">
        <location>
            <begin position="16"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="4">
        <location>
            <begin position="23"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="72" mass="7744" checksum="970F936C331E1693" modified="2018-12-05" version="1">CGPLYESRLTGEGGKHAANIAGAGVGRTGVFITLSIVLERLRYEGVVDIFQTVKILQTQRPAMVQTEVLLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYX4</accession>
    <name>A0A3B5KYX4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small integral membrane protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003240.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="73" mass="7930" checksum="DD6B8DA4E939BCD9" modified="2018-12-05" version="1">MESSGEGSVHYDCWNESNINMNVEASESTARRLYNRLCIGNTGIALKAVGALAALVGIYVIGYVTGYYVHRCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L028</accession>
    <name>A0A3B5L028_9TELE</name>
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        <name type="scientific">Xiphophorus couchianus</name>
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    <sequence length="426" mass="47072" checksum="E45716C5BC9EABDD" modified="2018-12-05" version="1">MSSTSFLFTLERLGRGSLLWNISDKNGKTTAEVFKNSCSHFFICIFLGSATVPIRGPGDGMETEKPPVTLEVITDSPPKHSPKSPHVTAPTTPQPLGVLHLGKVNREACTEVEAVRIIVPRAAITRSSRAGPTEGKGEAGQQAEEQGSPPLVDDWRGQLEKLQNSERRLLQDKEGLSNQIRVQTEVNRELKKLLVASVGDDLEYQFERLAREKNQLILENEALGRSLSHTAEQLERMSIQCDVWRSKFLAISKAELTNARAALQRQIREAQGAIQDLLLEREEFSRDMVSLEQLLVSLQWGRQQTYYPSAQPLSIGELAAANHKLADAINSHLLGKNNANSTVSKGSSAPAEHLCSTPAEKMAEKVLKVLNPITCSENKAEPAFSDSTSPNFLSSKKSIGRFHPYTRYENITFNCCERCTGDIIVL</sequence>
</entry>
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    <accession>A0A3B5L0Y0</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="350" mass="40347" checksum="98F570046259DE2E" modified="2018-12-05" version="1">GCRGEREPARAATEDDIPLGERKTVTDFCYLLDKSKQLFNGLRDLPQYGHKQWQSYFGRTFDVYTKLWKFQQQHRQVLDTRYGLKRWQIGEVASKIGQLYYHYYLRTSETSYLNEAFSFYSAIRQRSYYYQVNKEDRAELVVKKLRYYARYIVVCLLLNKMDLVKVLVKELSEEIEDYTQRFNTEDQLEWNLVLQEVAAFIEADPAVVLNDHNSMLVTSNRLQDGSVPPLEQGMVVGQLVLADALIVGNCNNQVKFSELTVDMFRMLQALEREPVNLATQTSKQATLVGFWLNAFTDKQPGRAPWVKARPPDARHRAPPPGLAPEWGPGDLRPGEGTPSPMFLSIIGVFK</sequence>
</entry>
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    <accession>A0A3B5L3A4</accession>
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        <recommendedName>
            <fullName evidence="6">FH2 domain-containing protein</fullName>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="FMNL_animal"/>
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    </dbReference>
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            <begin position="1"/>
            <end position="375"/>
        </location>
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        <location>
            <begin position="406"/>
            <end position="435"/>
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    </evidence>
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        </source>
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    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="464" mass="53031" checksum="0D407D217E3E24B9" modified="2018-12-05" version="1">VSVTNQMAKPNKIHSASNITLILLSIMDMEAFEEQFKTKAQAPPVDLGNLKKKMAGKTPSRVSLMEPNRAKNLAITLRKKCVATSDICSAIETYVLNFKTVTLSLDFLELLERFIPTEYEMKLIQTYESEGRSLNELSEEDRFMARFGKIPRLSQRISTLTFMGNFTESIQNIQPQLDAIIAASMSVKSSIKLKKILEVNIFIYILYFFFNCSADSFKLRDTKSTDRRHTLLDFIVRIIREKYPDLQNFYTELHFLDKAALVSFDSILRNLRALERGMELTRSEFSAEKGNPVLKTFLDTNSPLLDSLTAEEKTAQETYESVVEYFGEDPTMTPPSEFFSVFIRFIKVYKVRDFIAKKLSKQVLMFNSNESDSTLDFSFQTPIMCRQPQMDLIAELKRKQGSPPVREGKDGAIEDIITALKSVPFTARSAKRSSRLLCDSFFSDEVTSSHSEHYCVIDRRICPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L3K6</accession>
    <name>A0A3B5L3K6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">T-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="common">Monterrey platyfish</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L3K6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004431"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004484.1">
        <property type="protein sequence ID" value="ENSXCOP00000004431.1"/>
        <property type="gene ID" value="ENSXCOG00000003498.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166269"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001707">
        <property type="term" value="P:mesoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001756">
        <property type="term" value="P:somitogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20192">
        <property type="entry name" value="T-box_TBXT_TBX19-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.820:FF:000002">
        <property type="entry name" value="T-box transcription factor Brachyury"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002070">
        <property type="entry name" value="TF_Brachyury"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018186">
        <property type="entry name" value="TF_T-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF169">
        <property type="entry name" value="T-BOX TRANSCRIPTION FACTOR T-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00938">
        <property type="entry name" value="BRACHYURY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00425">
        <property type="entry name" value="TBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01283">
        <property type="entry name" value="TBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01264">
        <property type="entry name" value="TBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="8">
        <location>
            <begin position="39"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="253"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="263"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="392" mass="42729" checksum="55B33EFF7AD58720" modified="2018-12-05" version="1">MSSSSPDQRLEHLLSAVESEFQKGSEKGDVTERDIKLTLEDAELWGKFKELTNEMIVTKTGRRMFPVLRVSVSGLDPNAMYSVLLDFVAADNNRWKYVNGEWVPGGKPEPQSPSCVYIHPDSPNFGAHWMKAPVSFSKVKLSNKLNGGGQIMLNSLHKYEPRIHIVKVGGIQKMISSQSFPETQFIAVTAYQNEEITALKIKHNPFAKAFLDAKERYGSPICPNNSPPQFSSTASHPSGSYCERYSSLRSHRAAPYPSHYPHRSSNNYMDNNSGTLPTHDSWSALQIPNSTGMGTLSHTTNSTSNSGQYPSLWSVAGSSLTPSGSSSSSVPGGLTSQFLRGSSYTGLTSSLPVSSPSTMYEPSLSEVGVGDAQFENSIARLTATWAPVAQSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L484</accession>
    <name>A0A3B5L484_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein tweety homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Probable chloride channel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the tweety family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L484"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005256.1">
        <property type="protein sequence ID" value="ENSXCOP00000005195.1"/>
        <property type="gene ID" value="ENSXCOG00000004073.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183060"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005229">
        <property type="term" value="F:intracellularly calcium-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072320">
        <property type="term" value="F:volume-sensitive chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07912">
        <property type="entry name" value="Tweety_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006990">
        <property type="entry name" value="Tweety"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12424:SF6">
        <property type="entry name" value="PROTEIN TWEETY HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12424">
        <property type="entry name" value="TWEETY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04906">
        <property type="entry name" value="Tweety"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 13" id="KW-0868">Chloride</keyword>
    <keyword evidence="9 13" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="506" mass="56746" checksum="132807759F2BA743" modified="2018-12-05" version="1">MSLTRVDYIAPWWTYWLHNFPHINLRFHPTDKSFQPEDENYQQSLIFLGCVAAAGLGLNLLCLAVYLSFLCCCRKDEEEETKKPNSCCVTWSAVAAGLITCISVGVGFYGNSETNDGVYQLTYSLYNANHTLGGVDSLVSTMGNMRTGLHQHLARLDEIFATRGDYVQTLQFMQQMADNVIKQLLGLPDWDQAKVDLATIADQTANIEYYRWLTYLLLLILDLIICLLACLGLAKQSRWLLTTDFCVSPDKYVMGQTKGIITADIVHYYIYCNQTLSNPFQQPLTIFQRSLTTMQIQIHGLLQFAVPLFPTAERDLLGIQHLLNSSEASLHQLTALLDCRGLNKDYLDAIVGVCYDGVEGVLYLCLFSILAACAFTVMLCAIPRALRQIASRERDYDDIDEEDPFNPRARRIGSQNPNHTNMHSFCSFSSSMGSQNSLHPPAPTVSNPPMSEYMNQAALFGGNPRYENVPLIGRGSPPPSYSPTMRATYLSMTEEQSRHFGSDFQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L613</accession>
    <name>A0A3B5L613_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Guanylate cyclase activator 1C</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027900754.1">
        <property type="nucleotide sequence ID" value="XM_028044953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L613"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007753"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007851.1">
        <property type="protein sequence ID" value="ENSXCOP00000007753.1"/>
        <property type="gene ID" value="ENSXCOG00000005955.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114161591"/>
    <dbReference type="KEGG" id="xco:114161591"/>
    <dbReference type="CTD" id="9626"/>
    <dbReference type="GeneTree" id="ENSGT00940000164751"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008048">
        <property type="term" value="F:calcium sensitive guanylate cyclase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000052">
        <property type="entry name" value="Guanylate cyclase activator 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF80">
        <property type="entry name" value="GUANYLYL CYCLASE-ACTIVATING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0519">Myristate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="49"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="85"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="189" mass="21969" checksum="8089C364154DF415" modified="2018-12-05" version="1">MGSYSSNLDDILEEDMHHWYTKFMRESPSGLITLFELKTMLDMNGMTEEANSYVDQVFYTFDMDGDGYLDFVEYIAAISLLLKGEVNQKLKWYFKLFDQDGNGKIDKDELETIFKAIQDITRSYEIPPETIVTLIYEKIDIKGEGELTLEEFISGAREHPDIMDMLTRMMDLTQVLEIIVKGQRRNAVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6T7</accession>
    <name>A0A3B5L6T7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6T7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008023"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008122.1">
        <property type="protein sequence ID" value="ENSXCOP00000008023.1"/>
        <property type="gene ID" value="ENSXCOG00000006163.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166439"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032747">
        <property type="entry name" value="NUFIP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28333:SF2">
        <property type="entry name" value="FMR1-INTERACTING PROTEIN NUFIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28333">
        <property type="entry name" value="NUCLEAR FRAGILE X MENTAL RETARDATION-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15293">
        <property type="entry name" value="NUFIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="637" mass="68602" checksum="0C06033D0B69B8AB" modified="2018-12-05" version="1">MEEQPKDRAQDRQQQHHHNGEDRSSIQRTACLKHDQSQFQCQHQETQAKKTSKNVIINDEEGEKNPHLPYTVGMSHSSSSNGNRHISNSNVKQKGPQKHYISVQKVSSKFSEHKKNMDLKNDKEKALDLSNYEIQPSNKKDSALLQNGLVNCGLITNGYSSKDNDGSGSEGGYTTPKKRKSRCGNTKSADNVTKDKERDMQPRNTTPGASSVETPEKGTASRLDGFRSSYKAEAQTTAKRAVASETSVAEPQRKTSEGKAAGTFGKKAEEKHKGKLSSPSKEDSWTLFKPPPVFPVDNSSAKIVPKISYASKVKENLNKVAQGGGEAAPPPVRLSQVPMSAMKTITSASFTNGPVPGNGNGCPSVGTFFAPAASCVQSAPSVPSGENVASSLESSCSSTTSPVDGEASELRKCTVLIYPLNMQPVLPSARHLDPPAAQTNQKALGDIFQNQWGLSFINEPNPALDGGNGFVPAEDQTSVTSHNGSQAVPAKVAQPLFDISPFLEAEAFSQEAEKRTCAPCQVSSVCSPASTASEEETKLQPSVQEKTKVELKGVGSSLLAPSKDNGAKPAVGQQTTVLFGSSKEQIHPKDVGRRSSWGSFDLKAAVTYHTKMESIFNLQKQPKRIVFYDETKDGPDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8K3</accession>
    <name>A0A3B5L8K3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027888786.1">
        <property type="nucleotide sequence ID" value="XM_028032985.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSXCOT00000007244.1">
        <property type="protein sequence ID" value="ENSXCOP00000007152.1"/>
        <property type="gene ID" value="ENSXCOG00000005526.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114154155"/>
    <dbReference type="KEGG" id="xco:114154155"/>
    <dbReference type="GeneTree" id="ENSGT00390000008748"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040010">
        <property type="entry name" value="ZN608/ZN609"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21564">
        <property type="entry name" value="BRAKELESS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21564:SF2">
        <property type="entry name" value="ZINC FINGER PROTEIN 609"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="480"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="761"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="935"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1030"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1078"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1203"/>
            <end position="1286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1304"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="376"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="570"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="625"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="652"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="673"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="762"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="791"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="826"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="862"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="891"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="939"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1030"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1080"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1125"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1232"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1249"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1304"/>
            <end position="1317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1324" mass="143099" checksum="A257E88238060607" modified="2018-12-05" version="1">MSLSSSPAGGKGVDSNAVDTYDSGDEWDIGVGNLIIDLDADLEKDKLEMSSSKEGGGMAAPPGAVAALPDNIKFVSPVAAMQSKDGKCKSKRSKNSKESGKTTDGTKKEAQSRAQGDMAPQNTTSTLTKGTDKSGKASRIPPAIKKDKDVACGKTKKEKIEAVATRMVNTEKESGVLPLGAPRNSSFENQQNPELNPADQFGNLALDSAGIGQAVAMMTEQEEVDDADCRNLKKVASNEKMESPFSTCAPPPLHLLGPPANSSDISSPCEQIMVRTRSVAVNTAEAALATEPECLGPCEPGTSVNLEGIVWQETEDGMLVVNITWRNKTYVGTLLDCTRHDWAPPRFCESPTSDMEMRSGRGRGKRMRPSTNTPLNDHSNSSDSKGSGSKTRGAAATSKGRRGVVVGCGEDAKASPSSAKRKTKPVSDMEPTSSSEDSKASKRMRTNSSGVGTLVPVGKTEALAPPQLERTCPSPVLIDCPYPNCNKKYKHINGLKYHQARAHNDHDVRLDQDGDSEYGDDSVLQHDPTSCNGAAFSPARSNTPKGRGFDAPSPSSGKQTLKGKKKAGDGEPEATDGGEEGGCLTDEASNDGVDDRKARRMSVNSKPDKSSQKSAKMGRPLPLGVASSYSPQAPSPALSSVVQSVPSSPQLKNSQTKPSPLSDPSCSPTLGKDKKKKDKKKRDSWKEEDSPVAMNKIGRLEEGKSPYSETTDSLLNGCMEAHQSRLASIKAEADKVYSFSDNAPSPSIGVASRIEAGLATPLHQNQNGADNVSVKTSSPAYSDISDAGEDGEGKAEGVKVKPDPDQGPREGAKKTLFPSQAPSKESPYYPNYDSYYSPSYPNPSPGAVPGATAHMEATQVKVKKEEDLEVVEEGKLKVEPPEERKAELGPQQPSVIQPRSNMYSQPLYYNQYYVAPYSYASDPAYHNHLLASNPAYRQQYEERQRQSDKKLEGKDRDSIGRDEWKQKTSGPPSVSGAPGLTDLGSKGLVTPAKSKDPAAASEQAKSVIMAKGEEMKLPSTQAEGLKMKLHETSHHGKEEAKPGIELGRPTGVDSTMWYRQEPDSHLWPYVYPSKYSEAARQQEEERWKDERERERKAKEDRPRVKESLQKDEVKEGVEGRTLLPPEEHQGGGKEVRVPHMQFASPLAQHQGYMSYMQGPYGYSQGYDPSHPGYRGMPAVMMQNYPASYLKAGYPFSSYGGKVAGGEDGEKPSRSSPTVKPPSEAKALDLLQQHASQYKSKSPSVQDNKTAQERERDWERDREREREGARPRSSPSHRVIPPHHHLGYPLLSGQYDLSYAAGLSSSAIVASQQASAPSMYPPPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9I6</accession>
    <name>A0A3B5L9I6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9I6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004489"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004545.1">
        <property type="protein sequence ID" value="ENSXCOP00000004489.1"/>
        <property type="gene ID" value="ENSXCOG00000003552.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159666"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000337">
        <property type="entry name" value="Solute carrier family 16 member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF21">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="33"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="394"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="423"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="36"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="459"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="484" mass="52142" checksum="3E2A2CB823006B45" modified="2018-12-05" version="1">AEAAADCERGRGSESGDPVAITGGAVTAPDGGWGWAVLVATILVLAMTLGFPSCVGIFYTDLQNEFHASNSETSWVPSIMTSALHAGGPFCSVLVGKLGCRATVMLGGVLSGLGMAASSFTNSIAELYVTAGVITGLGFCFSFQPAVTILGHYFVRRRAFANAMSSTGTALGLCTLPFLGNYLHSEFGWRGSFLILGAVLLNCCVCGAVMRPLQPKKHRGQPLMDHTPPPSEKQTKRELRLETRWRSLVASVKEHMAFDQFLNNRHYRVFAIGITFMMLGFVVPLIYLVPYATAYGMEPSRAALLLSILGIVNIMVRPPFAIMFNMPWFKGRHVYVFASALLFNGLSNCICCIGAGFPVLVAYVVTYGLSMSVVGSLMFTVLMDIVEMRRFPSALGLLAVMESITLLIGPPLAGVLVDTTQQYFYVFIACSAVVASSALFLILSFLWLDKKERKASLQGQLSAQPEAEKPAADAPPVCEYSDRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9I7</accession>
    <name>A0A3B5L9I7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cerebral endothelial cell adhesion molecule</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 25 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9I7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006416"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006496.1">
        <property type="protein sequence ID" value="ENSXCOP00000006416.1"/>
        <property type="gene ID" value="ENSXCOG00000004966.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234558"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd06532">
        <property type="entry name" value="Glyco_transf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000048">
        <property type="entry name" value="Glycosyltransferase 25 family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050757">
        <property type="entry name" value="Collagen_mod_GT25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002654">
        <property type="entry name" value="Glyco_trans_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10730:SF9">
        <property type="entry name" value="INACTIVE GLYCOSYLTRANSFERASE 25 FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10730">
        <property type="entry name" value="PROCOLLAGEN-LYSINE,2-OXOGLUTARATE 5-DIOXYGENASE/GLYCOSYLTRANSFERASE 25 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01755">
        <property type="entry name" value="Glyco_transf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 25" evidence="7">
        <location>
            <begin position="354"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="600"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="623" mass="72460" checksum="0967FDEFA3F2A791" modified="2018-12-05" version="1">MTEICALSLSLSLAGEKICFQIVLGQFLTVCVLCVLWWLGFTLTSRAECYFVEEKFPEESGMQPPTVVIAIIARNAAHSLPYYLGALERLNYPKDRISVWAATDHNSDNTTAILREWLTVMQRFYHYVEWRPMDQPTSYAGEFGPKHWPNSRYEYVMKLKQAALNFARKRWADYILYADTDNILTNAETLNLMIAENKSVIAPMLDSPGAYSNFWCGITPQGYYRRTAEYFPTRYRHRHGCYPVPMVHSTLLLDLRKEGMKKLAFYPPHEDYSWPYDDIIVFAFSCRAAEVQMYVCNKERYGYLNVPGKPHFTLEDDRLNFVHVHLESLIDGPTMYPSRYVHMFPKQRDLVGFDEIYLINLRRRPDRRDRMLYSLNELEVDVKVVDAIDGNALNSSDIKFLGVDLLPGYYDPFSGRTLTKGEVGCFLSHYYIWKEVVDMQMDKALIFEDDVRFEANFKRRVLRLMEEVEQVELDWDLIYLGRKQVNPGKEVAVENVRNLVVADYSYWTLSYAISQQGAQKLLNAEPLSKMLPVDEFLPIMYDKHPNEDYKSHFPNRNLQAFSTRPLLVQPCQYAGDPEWVSDTETSTLWDDDSVRTDWRGSHKTLKGAAPPPEMLSASYRDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9T4</accession>
    <name>A0A3B5L9T4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycoprotein hormone beta-5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Thyrostimulin subunit beta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Functions as a heterodimeric glycoprotein hormone with GPHA2 able to bind and activate the thyroid-stimulating hormone receptor (TSHR), leading to increased cAMP production. Plays a central role in controlling thyroid cell metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer with GPHA2; this heterodimer interacts with thyroid-stimulating hormone receptor (TSHR), and hence stimulates cAMP production.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycoprotein hormones subunit beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9T4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006506"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006586.1">
        <property type="protein sequence ID" value="ENSXCOP00000006506.1"/>
        <property type="gene ID" value="ENSXCOG00000005040.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111179"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002155">
        <property type="term" value="P:regulation of thyroid hormone receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00069">
        <property type="entry name" value="GHB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000029">
        <property type="entry name" value="glycoprotein hormone beta-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006208">
        <property type="entry name" value="Glyco_hormone_CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001545">
        <property type="entry name" value="Gonadotropin_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11515">
        <property type="entry name" value="GLYCOPROTEIN HORMONE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11515:SF14">
        <property type="entry name" value="GLYCOPROTEIN HORMONE BETA-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00007">
        <property type="entry name" value="Cys_knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00068">
        <property type="entry name" value="GHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0372">Hormone</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycoprotein hormone subunit beta" evidence="12">
        <location>
            <begin position="16"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="111" mass="12802" checksum="899E917954370AD7" modified="2018-12-05" version="1">TVVCVSMTTPLQSFRGCAVREFSFVAQKPGCKGLHITTEACWGRCHTWERPIIEPPYIHRHHRVCTYSRIRHMTARLPGCLPNVSPLYHYPMALQCHCTFCSMEDTECQTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAG5</accession>
    <name>A0A3B5LAG5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phospholipid phosphatase-related protein type 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Inactive 2-lysophosphatidate phosphatase PLPPR1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Lipid phosphate phosphatase-related protein type 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May play a role in neurite outgrowth and neurogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Neuron projection</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAG5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006731"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006814.1">
        <property type="protein sequence ID" value="ENSXCOP00000006731.1"/>
        <property type="gene ID" value="ENSXCOG00000005209.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158875"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03384">
        <property type="entry name" value="PAP2_wunen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000005">
        <property type="entry name" value="phospholipid phosphatase-related protein type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF41">
        <property type="entry name" value="PHOSPHOLIPID PHOSPHATASE-RELATED PROTEIN TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="15">
        <location>
            <begin position="97"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="294" mass="32214" checksum="EB1F4E92A1272A71" modified="2018-12-05" version="1">MFFVQLVIMSGTVLLAYYMECTDLFSVHVQGFFCNDAELMKPYPGTEESSFVPPLILYCVVAAAPTVIVTLLAQEKTIVTGDCCYLNPLIRRIIRFIGVFAFGLFATDIFVNAGQVVTGGLSPYFLSVCKPNYTGTECRFNHQFIVNGNICTGNPIVVENARRSFPSKDASLSVYSAVYLTMYITSTIKTKSSRLAKPVLCLGTLCSAFLTGLNRVSEYRNHCSDVVAGFILGSAIALFLGICVVNNFKGVQNPATKQKTEEYRGLPLMTFPRVESPLETLSAQNHSASMTEVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAR9</accession>
    <name>A0A3B5LAR9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Centromere protein O</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-O/MCM21 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAR9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005024.1">
        <property type="protein sequence ID" value="ENSXCOP00000004964.1"/>
        <property type="gene ID" value="ENSXCOG00000003887.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016702"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031511">
        <property type="term" value="C:Mis6-Sim4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd23836">
        <property type="entry name" value="DRWD-C_CENP-O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23835">
        <property type="entry name" value="DRWD-N_CENP-O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018464">
        <property type="entry name" value="CENP-O"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14582:SF1">
        <property type="entry name" value="CENTROMERE PROTEIN O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14582">
        <property type="entry name" value="INNER KINETOCHORE SUBUNIT MAL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="276" mass="31159" checksum="89C106A0F9C4D4B3" modified="2018-12-05" version="1">KCFLPPGVLSHLSLLEVQARSRGSQVPQQPSRVKELKAKVEALTSQRDQLKAEAPVDKQRVDGEDEEMDVDSESSELFHLMARHSELMDLLHAHNLIGGYDAITTNRGKSMCFSLATQYEGVYLDTYNLELNLKPNVRISRHNIPPFIPLNSLAEQSDLQTDVRAFLATVSQHLNAFAGRRQQLKLVKEQHKSVEVMESNLLCSILVLMFTVPKKTPLLCTLEYTDHTRCLPTQVHLNCEDELLPDSPKWKKNCSLLMEVPVHRALTAIKKASDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAS0</accession>
    <name>A0A3B5LAS0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase M1 membrane alanine aminopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAS0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006826"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006909.1">
        <property type="protein sequence ID" value="ENSXCOP00000006826.1"/>
        <property type="gene ID" value="ENSXCOG00000005280.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155211"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000014">
        <property type="entry name" value="aminopeptidase O isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033577">
        <property type="entry name" value="AOPep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46627">
        <property type="entry name" value="AMINOPEPTIDASE O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46627:SF1">
        <property type="entry name" value="AMINOPEPTIDASE O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="2">
        <location>
            <begin position="40"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="352" mass="40931" checksum="2D0BAB6C14FD2FD8" modified="2018-12-05" version="1">ALQNFMSFVFYSPHIIFLSQSVLCKECPGSGLNRLTLSGSRICHEIAHSWFGLVIGARDWTEEWISEGFATFLEDIIWAQAQQVLVPKNEYTAEQFDLKALLRWRRLSDELQNSNEELQILRPNMERTGQVSDSGSSTIKHALNPDKTFMQVHYLKGYFLLRFLASEVGDQHLIDFFRLFVKKYHGQLVLSQVRPKYFREIFLSASIDVLQSLFITSSCFFSLFWLQVRHRWCELVVKHTYSQAYCDVEDFLLHDQTLFHVPMDHGMLSFLPPWVLPRLLSCRHSFFKQVHCVLPFFPRCHSAPCFFLHLPVLLIIFLVTTFLPCALLSLLPSFLTLILMSFLSSTVPSFIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBX5</accession>
    <name>A0A3B5LBX5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBX5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009797.1">
        <property type="protein sequence ID" value="ENSXCOP00000009683.1"/>
        <property type="gene ID" value="ENSXCOG00000007368.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183052"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000012">
        <property type="entry name" value="RB-associated KRAB zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000966">
        <property type="entry name" value="ZFP90 zinc finger protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001384">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002110">
        <property type="entry name" value="Zinc finger protein 1053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000759">
        <property type="entry name" value="zinc finger protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000512">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001325">
        <property type="entry name" value="zinc finger protein 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000303">
        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050752">
        <property type="entry name" value="C2H2-ZF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384">
        <property type="entry name" value="FINGER PUTATIVE TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384:SF218">
        <property type="entry name" value="ZINC FINGER PROTEIN 502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00614">
        <property type="entry name" value="ZnF_BED"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="22"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="163"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="303"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="331"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="415"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="443"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="472"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="500"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="528"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="556"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="584"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="612"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="640"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="667" mass="76068" checksum="727CB8F300FC83C0" modified="2018-12-05" version="1">MSIREPEPIRDRLQNASNKSCQKSRVQSDDAHGSKHRRRSRSLSQTEKSLFSCKVCGKSFSQSSHLNNHMRSHASKKQYPCEVCGKFLNDKGSLTVHMRTHTGEKPFTCPTCGKSFGARSSFIAHVRTHTGEKPFPCPSCEKSFIVKSHLITHMRIHTGEKPFQCLTCGKRFGAKSNLNTHMRTHTGEKPFACVSCGKSFSVRSHLITHLRTHTGEKPYSCPYCGRSFSLRSHLNVHMSTHTGEKPFPCVACGKSFRVRSSLTIHMRTHTDEKLHICDVCCKGFTQSSRLASHMRIHTGERPFPCPTCGKSFSAKTYLNTHMRTHTDEKSYLCVICGNSFSRKSNLNRHMRTHTGEKPYDCEVCGKSFGCSGSLTVHMRSHTGEKPFPCVTCGKSFSVRSYLVIHMRTHTGEKPFPCVTCRKSFLLRSHLTRHMRVHTGEKPFPCSTLCGKRFRNSCGLTKHLRIHTGEKPFTCQTCGMQFRFQYAMRRHRKIHTGEKPFSCKTCGKSFIRKFALLTHMRVHTGEKPYSCSVCGKCFRCSSDLSKHTSIHSSEKPYSCETCRKSFTTKRSLILHSRCHSEEKPYFCPICHKGFRRSDNLSVHMRTHMDEKLFTCEFCGKSFNQNVHLVIHRRTHTGESPFPCSMCEKRFKNRSALNSHLRLHAADPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCN4</accession>
    <name>A0A3B5LCN4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RAB GTPase activating protein 1 like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCN4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008300"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008399.1">
        <property type="protein sequence ID" value="ENSXCOP00000008300.1"/>
        <property type="gene ID" value="ENSXCOG00000006354.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154611"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47728:SF1">
        <property type="entry name" value="RAB GTPASE ACTIVATING PROTEIN 1 LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47728">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="112"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="40420" checksum="A411761D697A2994" modified="2018-12-05" version="1">DPSPLPCPTPPPSPPRSPPIMEALCLVFVLHVSHPPLLVSLQTSKEDLLQADFEGALKFFRVQLPKRYRAAENARRLMEQACNIKVPTKKLKKFEKEYQTLRESQLQQEDPIDRYQKENRRLQEASMRLEQENDDLAHELVTSKIALRNDLDQAEDKADVLNKELLSTKQRLVETEEEKRRHEEETAQLKEVFRRELEKAEMEIKKTTAIIAEYKQICSQLSTRLEKQQAATKEELDIVRNKVMSCEHCRDLFSPVGSLQASSPGGDGTSSEPLDEEKDGLKEQLRQLELELAQTKLQLVEAKCRIQELEHQRGVLMTEIQAAKNSWFSKTLGSLKTGPPPLCPPYIPLQDAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCN5</accession>
    <name>A0A3B5LCN5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Myc target 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCN5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005634"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005700.1">
        <property type="protein sequence ID" value="ENSXCOP00000005634.1"/>
        <property type="gene ID" value="ENSXCOG00000004391.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011642"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035162">
        <property type="term" value="P:embryonic hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029180">
        <property type="entry name" value="Myc_target_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14869">
        <property type="entry name" value="MYC TARGET PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14869:SF0">
        <property type="entry name" value="MYC TARGET PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15179">
        <property type="entry name" value="Myc_target_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="195" mass="21300" checksum="617A499DF0284611" modified="2018-12-05" version="1">MAENNTNVILDILKSFDAPLIIAFCISIAVGLLLGATVYLILTWMSRRKAGSASITRRPPRRSQNSPRSRPGFNRSSSYERRSNNSLISAAFSFHRPTSSPDHLDAVGHKSSFRASTFHPLIQCSQIAREAEEGSQTTLPRTPPLNTSPGLAQTAAEPAVNHSRPESFWGNNGLRGLGVTQTPPPAYESIIRAYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCY4</accession>
    <name>A0A3B5LCY4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Calcium release-activated calcium channel protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Protein orai-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Transmembrane protein 142A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orai family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCY4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005734"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005800.1">
        <property type="protein sequence ID" value="ENSXCOP00000005734.1"/>
        <property type="gene ID" value="ENSXCOG00000004469.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015354"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015279">
        <property type="term" value="F:store-operated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002115">
        <property type="term" value="P:store-operated calcium entry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.140:FF:000001">
        <property type="entry name" value="Calcium release-activated calcium modulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.140">
        <property type="entry name" value="Calcium release-activated calcium channel protein Orai"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012446">
        <property type="entry name" value="CRAC_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038350">
        <property type="entry name" value="Orai_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31501">
        <property type="entry name" value="CALCIUM RELEASE-ACTIVATED CALCIUM CHANNEL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31501:SF3">
        <property type="entry name" value="CALCIUM RELEASE-ACTIVATED CALCIUM CHANNEL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07856">
        <property type="entry name" value="Orai-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0391">Immunity</keyword>
    <keyword evidence="15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="113"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="225" mass="25060" checksum="F65151EB3A63B70A" modified="2018-12-05" version="1">MKICLNEHSLQALSWRKLYLSRAKLKASSRTSALLSGFAMVAMVEVQLDNKYPYPPALLIAFSACTTVLVAVHLFALMVSTCILPNIEAVSNVHNLNSVKESPHERMHRHIELAWAFSTVIGTLLFLAEVVLLCWVKFLPIKPDQSNNSTVSSGVAAAITSTSIMVPFGLIFIVFAVHFYRSLVSHKTDRQFQELEELSNLTRLQNELDNRGESSILQSPSSQFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LD16</accession>
    <name>A0A3B5LD16_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">SH3 and PX domains 2B</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SH3PXD2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LD16"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007586"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007682.1">
        <property type="protein sequence ID" value="ENSXCOP00000007586.1"/>
        <property type="gene ID" value="ENSXCOG00000005846.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158396"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016176">
        <property type="term" value="F:superoxide-generating NADPH oxidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
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    <dbReference type="GO" id="GO:0060349">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0061448">
        <property type="term" value="P:connective tissue development"/>
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    <dbReference type="GO" id="GO:0042554">
        <property type="term" value="P:superoxide anion generation"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SH3 and PX domain-containing protein 2A"/>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="4"/>
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    <dbReference type="PANTHER" id="PTHR15706:SF26">
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    </dbReference>
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        <location>
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            <begin position="282"/>
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        <location>
            <begin position="439"/>
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        </location>
    </feature>
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        <location>
            <begin position="126"/>
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        </location>
    </feature>
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            <begin position="290"/>
            <end position="309"/>
        </location>
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        <location>
            <begin position="484"/>
            <end position="499"/>
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        <source>
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    <sequence length="885" mass="98462" checksum="94CC4EC275970EB9" modified="2018-12-05" version="1">MPRRTVQEVTVQDVQKRRSPNKHYVYIIKVSWSDGSTETIYRRYSKFFDLQMELLDKFPVEGGQKDPKRRIIPFLPGKILFRRSHIRDVAMKRLRPINEYCRALIQLPVYISQCEEVRVFFETRPEDLNPPKEEPIGKKKSGDPTSADALFLEQYVAVTDYEKQESTEVSLHVGQTVEVIEKNESGWWFVSSEDAQGWVPATCLEAQDDPDDFTIPGEEEEKYSVIYPYSARDQDEIDLERGMIVEVIQKNLEGWWKIRYQGSEGWAPASYLKKSDIQSQRQSAGAAAHASTNDLEGVSKQNLQNNASRDNQKENRLSIFSDNNKIKVGSRHGLPPRRDLTIPRGANLPKPPVPPKVEEEYYTIADFQTTIPDGISFQAGVKVEVVEKNTSGWWYIQIDEKEGWAPATFIDKYKKTSNALRPNFLAPLPNEMEKLRLEDGGADDDTWSKPLPDEPTTASDSFARPKLRDWKSSANKTTPAFAGPLPPAPAAPPAPAPPPAEEKPALPPRRESINKSFELEAAEKPKPDHCKPLPPKPPAPGVIVPLVTPKSAPFKPEKPPEIKKEDKSKALPLRNEMGLECGHKVSAKEVKKFNLKPVPKQPPKPKAENREEKPEPAGPAAFMKGKPVVRPKPVPAAKPDPPAENKVDITNLRSKLRPSKAADVGSPSSEGNHQNDSSAAGSSPPPSSSKFSDEPKLPPKHINGLSQEKSSPPPKPAVPPHKEPPQRPPAMAPKRPPPPKKTDPSSPTENKPPSLREPQETPKPGPPPQLSRPPPPKPKAFVQPPSSKEKDGPKGNKAPIPPKPQMKHEKSGPPKDKLPPLLKEPSKDELYLAVADFEGDEQTSGFKVGTVFEVLEKNSSGWWFCKNVGEDVEGWIPSNYLSKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDV2</accession>
    <name>A0A3B5LDV2_9TELE</name>
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        <submittedName>
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        </submittedName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <text evidence="1">Belongs to the opioid growth factor receptor family.</text>
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        <property type="entry name" value="OGFr"/>
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        <property type="entry name" value="OPIOID GROWTH FACTOR RECEPTOR OGFR ZETA-TYPE OPIOID RECEPTOR"/>
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            <begin position="97"/>
            <end position="300"/>
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            <begin position="27"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    <sequence length="386" mass="43726" checksum="325751BD66968861" modified="2018-12-05" version="1">MGNLVGSWRFKEPSTVEECDSTWGSDSEGDEPTAEDDSGISDSVSPAESERAAGHSGDAALQVHGGKSAPKMKRSFYAARDLYKYRHSYPNFRKSRQPNEYRNLRFYMNKIPLVPDGIYIEEILSKWRGDYDKLEHNHTYIQWLFPLREQGLNFYAHELTQEEIKEFQSTREAKRRFLAAYSMMLDFYGIKLLDKSGNVARAPNWQERFQHLNESHHNYLRITRILKSLGELGYEAFKAPLVRLFLEESLCSGTLPNVQHSVLEYFVYTIRLPATRRRLLRFARKHYRPAHAFLWGPPSKKGACSGSGVGGGSSGIRAPAPTPEHHRRGEENTASSRINVSSHDAIMCQDLVGGGIKGFPDLGSYEAGVPGQLMLVSGKRERSDFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LE21</accession>
    <name>A0A3B5LE21_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulator of microtubule dynamics 2</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Poeciliinae</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="entry name" value="RMD1-3_a_helical_rpt"/>
    </dbReference>
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        <property type="entry name" value="RMD1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="103"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="253" mass="27990" checksum="D1828012C7E1BAD8" modified="2018-12-05" version="1">MAQTDSKVLVLGALAGVAGISLAVVCYRGLRSKRRVSWPGFYQNRTNDPGSGLLLVDGPVLPSGQAEVLERLEALIQCVSELKEEMKALKTALPSLPDQVREELKGHSEVRRVSAQHRTAATRRKRTAGATSSPGGQSSEDAESEGGYMTALTDSEDEEQSDEEQTQGKLSVLLDRIDRLRQGGESDKRESLSVLLEQRDEFGQNSEFLWRLTRAYCDVHDITVTLEEKKTHAENGNLQTSLQYFTKRLTLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEP2</accession>
    <name>A0A3B5LEP2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 17">Receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2 17">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2 17"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LEP2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009040"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009149.1">
        <property type="protein sequence ID" value="ENSXCOP00000009040.1"/>
        <property type="gene ID" value="ENSXCOG00000006878.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156107"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009925">
        <property type="term" value="C:basal plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038132">
        <property type="term" value="F:neuregulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038131">
        <property type="term" value="F:neuregulin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd12095">
        <property type="entry name" value="TM_ErbB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.20.20:FF:000013">
        <property type="entry name" value="Erb-b2 receptor tyrosine kinase 3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000001">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000002">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.20.20:FF:000004">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000233">
        <property type="entry name" value="receptor tyrosine-protein kinase erbB-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000276">
        <property type="entry name" value="Receptor tyrosine-protein kinase erbB-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.20.20">
        <property type="entry name" value="Receptor L-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044912">
        <property type="entry name" value="Egfr_JX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006211">
        <property type="entry name" value="Furin-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032778">
        <property type="entry name" value="GF_recep_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000494">
        <property type="entry name" value="Rcpt_L-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036941">
        <property type="entry name" value="Rcpt_L-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016245">
        <property type="entry name" value="Tyr_kinase_EGF/ERB/XmrK_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF88">
        <property type="entry name" value="RECEPTOR TYROSINE-PROTEIN KINASE ERBB-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00757">
        <property type="entry name" value="Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14843">
        <property type="entry name" value="GF_recep_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01030">
        <property type="entry name" value="Recep_L_domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000619">
        <property type="entry name" value="TyrPK_EGF-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 17 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12 17" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="615"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="950"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="683"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="978"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1047"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="978"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1054"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="1069"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="689"/>
            <end position="697"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18 19">
        <location>
            <position position="716"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR000619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000619-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1180" mass="132086" checksum="C43B0503A98D8662" modified="2018-12-05" version="1">CPNNMTLTPLEDFLTFLLPEVCPGTQNGLSSTGSQETQYNLIKDRYSGCEIIMGNLEITQIESNWDFSFLKTIREVTGYVLIAMNHVQEIPLGQLRVIRGNTLYERRFALSVFFNYPKEGSNGLRQLGLLNLTEILEGGVQIINNKFLSYGPLIFWQDIVRDNSAPIDIQNNGERGPCHKSCGDYCWGPNKDQCQILTKMVCAPQCNGRCFGRSPRDCCHIECAAGCKGPLDEDCFACRHFNDSGACVPQCPQTLIYNKQTFQMETNPNAKYQYGSICVSQCPTHFVVDGSSCVSVCPPDKTEMERDGQRQCELCSGFCPKVCNGTGAEHRQTVDSSNIDSFVNCTKIQGSLHFLVTGILGDDFKNIPPLDAKKLEVFRTVREITDILNIQSWPKELNNLSVFSSLSTIQGRSLYKRFSLMVMRIPTLTSLGLRSLRYINDGSVYISQNTNLCYHHTVNWTQLFRGRRVRVNNLNNNKPFAECVTEGHVCDPLCSDSGCWGPGPDQCLTCRNYSRHGTCVGSCSFLSGSAREFARSDKECVACHPECKPQRGKVSCTGTGADECVACSNLQDGPYCMSSCPSGVFDEHRGLIFKYPNKEGHCEPCHPNCTQGGQITGIALAVPACLIFCLGLFFLGVLYHRGIAIRHKRAMRRYLESGESFEPLGPGEKGTKVFARILRPSELKKMKLLGYGVFGTVHKGFWTPEGETVKIPVAIKTIQDSSGRQTFTEITDHLLSMGSLDYPYIVRVLGICPGPSLQLVTQLSPRGSLLEHIRQHKNSLDPQRLLNWCVQIAKGMFYLEEHRMVHRNLAARNILLKNDYQVQISDYGVADLLYPDDKKYVYTDTKTPIKWMALESILFQRYTHQSDVWSYGVTVWEMMSFGAEPYVSVQPQEVPSLLEKGERLSQPHICTIDVYMVMVKCWMIDENVRPTFKELASDFTRMARDPHRYLVIKVQMMLFLVSELFFNLDLTSSVRTLPERSEVRGNGKEAEGLRAGSLRRTRLGSERSSPRVALSRHRKLSTASTPSSYKVWTAQEDEEDLDHYGYVLPGSPETPERKGGESELERCRSQTSSTTGAETDQTAGGLVKERKGEEETEICHTNKQAAVTEDPSGGVVPGADVLAAGAGTVEEYEEMSRFEKVPGERGHVCAGIREPGNNTSFDNPDYWHSRLFLKPDAVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LF18</accession>
    <name>A0A3B5LF18_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane protein 179Ba</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TMEM179 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LF18"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006454"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006534.1">
        <property type="protein sequence ID" value="ENSXCOP00000006454.1"/>
        <property type="gene ID" value="ENSXCOG00000004995.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048151"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059010">
        <property type="entry name" value="TMEM179-179B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029776">
        <property type="entry name" value="TMEM179B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31056">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 179B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31056:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 179B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26158">
        <property type="entry name" value="Claudin_TMEM179-179B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="196"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="229" mass="25029" checksum="4316DD8AD98D92D9" modified="2018-12-05" version="1">MTVSVLLVLELSLYTCCFVCGIVAAASLTIVQGNFGGLCMLYGRVSYNATGSVVGVQASTSASLCYFVSAISIMVAVVCFSLSVYWVYAFCMEGEMRRERLWLNVMVFVSGIFLFFLLITGCMLKIGRDSLCASILRTVTDVKSCDEVQSKNWVSPLQGTRIYTNLHKSETAVWVNFFFWIIIGALVIVQKRQSSGAKSVPTPAGSLFGEPGATAAETEPFFNRPPRPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFD3</accession>
    <name>A0A3B5LFD3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 13 member 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. NADC subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFD3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009396.1">
        <property type="protein sequence ID" value="ENSXCOP00000009285.1"/>
        <property type="gene ID" value="ENSXCOG00000007072.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234550"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015139">
        <property type="term" value="F:alpha-ketoglutarate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015138">
        <property type="term" value="F:fumarate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017153">
        <property type="term" value="F:sodium:dicarboxylate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015141">
        <property type="term" value="F:succinate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071285">
        <property type="term" value="P:cellular response to lithium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10283:SF82">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 13 MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="6" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="297" mass="33473" checksum="9B12651EE12E8AC0" modified="2018-12-05" version="1">MALYWCTECMPLAVTALLPVVLFPMMGIMKAGDVSIEYLKDSNMLFVGGLLVAIAVEHWNLHKRIALRVLLLVGVRPALLMMGFMIVSAFLSMWISNTATTAMMLPISQAVLHQLFASSDSRVHEGEHEERNRGKCVLLWFKLFSCFCSSNHYFFTQRLFPGNGDVINFASWFGFAFPNMVLMLAISWLWLQFMFLGFNLKKSFGCGMKSDRDKEAYAVMKEEYKKLGGMKFAEGAVLTIFILLVLLWFTREPGFIPGLSPCSGGRRFDSRPGDICRMSSPSLITLFPVKLLSNKGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGB0</accession>
    <name>A0A3B5LGB0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Arginine vasopressin receptor 2a, duplicate a</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027873625.1">
        <property type="nucleotide sequence ID" value="XM_028017824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGB0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008711"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008817.1">
        <property type="protein sequence ID" value="ENSXCOP00000008711.1"/>
        <property type="gene ID" value="ENSXCOG00000006653.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114144727"/>
    <dbReference type="KEGG" id="xco:114144727"/>
    <dbReference type="GeneTree" id="ENSGT01050000244882"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005000">
        <property type="term" value="F:vasopressin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045907">
        <property type="term" value="P:positive regulation of vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001992">
        <property type="term" value="P:regulation of systemic arterial blood pressure by vasopressin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15388">
        <property type="entry name" value="7tmA_V2R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001817">
        <property type="entry name" value="Vasoprsn_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF20">
        <property type="entry name" value="VASOPRESSIN V2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00896">
        <property type="entry name" value="VASOPRESSINR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="89"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="478"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="482"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU046427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="497" mass="55615" checksum="4AC7A5C8A6FD7FED" modified="2018-12-05" version="1">MERISVETDWDGLGLSSPGAKGRNNISSFVTELNSFNISRGGGSLFGILIENGSNATSHTVPQPRARDQGLARAEIAILGVVLALTTLGNSFVLWVLLRRRKHNAPMHLFMVNLCVADLVVALFQVLPQLIWDITERFQGPDFLCRSIKYLQIVGMFASSYMIVAMTVDRHHAICCPLQAYRVGAMSRWNTPVIVAWALALVLSIPQVFIFSRSEVAPGEFECWGHFTEPWGLKAYVTWMTVAVFLLPALIITICQIRIFREIHNNIYLKSERIMMAELKRSEILLRFQGLRKEDEREGERGRQATGREGGGRLLKDNDAHDCKVGEHYDYVPSVIQYSSCCGEHQTAATTLMQEQIQNGSDFRESCANSARCSLDHAPPPHQVTPVPSITKAMSKTVRMTLVIVLVYTVCWSPFFIVQLWAAWDPDPPDQGVAFTILMLLASLNSCTNPWIYTAFSSSVSRELQNLLQCRSRLGRRGSLPDDSTATHTSTTKDNLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHJ9</accession>
    <name>A0A3B5LHJ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VWFD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Atherinomorphae</taxon>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHJ9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009156"/>
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        <property type="entry name" value="TILa_dom"/>
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        <property type="entry name" value="VWF_type-D"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="VWFD" evidence="4">
        <location>
            <begin position="71"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="143" mass="15552" checksum="A900805F36964676" modified="2018-12-05" version="1">MDGVCVPHSDCGCSFEGHYYRSGETVILDADCGRRCTCSYGSMTCSSHSCGQHESCRVEDGVRGCTPNSFATCWIRGLGSYNTFDGVMYQYPGACRLTLAKVMGSSDRTHFMITVEKVPQGSQGFSNVLKWLPGRSLCGKQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHY9</accession>
    <name>A0A3B5LHY9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHY9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010225"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010348.1">
        <property type="protein sequence ID" value="ENSXCOP00000010225.1"/>
        <property type="gene ID" value="ENSXCOG00000007759.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="DUF4559"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35083:SF2">
        <property type="entry name" value="CHROMOSOME 17 CXORF38 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35083">
        <property type="entry name" value="RGD1565685 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15112">
        <property type="entry name" value="DUF4559"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="267" mass="31122" checksum="32A3ED77B0CFAEA2" modified="2018-12-05" version="1">MGREELTFRLNDEEYKNWLKAGRCLLILKDGLLPFTDQQLRAFHGDLLNQYPPLRRPCETSSCKPRGNTVGFDPLFPVCAEWQRVILKHHRQPDGTIHWTNCFPSYWRTDHWELAKAYMPRGQTKVSRADEFDASALLNLINYCDCFSSVDPKLVRQVIQCRNELMHSCEFRVKDDWMRRYRTALRHFVQQLSFVPHMLTVGRQIEDVSLFGEQRQLHVRALYGCASIANQLKRLRGFLQANKDLGEQFSSELQAIDSLEAKGGEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKV8</accession>
    <name>A0A3B5LKV8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKV8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011305"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011436.1">
        <property type="protein sequence ID" value="ENSXCOP00000011305.1"/>
        <property type="gene ID" value="ENSXCOG00000008512.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:perinuclear region of cytoplasm"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:WW domain binding"/>
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    </dbReference>
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        <property type="term" value="P:metal ion transport"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22305">
        <property type="entry name" value="NDFIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019325">
        <property type="entry name" value="NEDD4/Bsd2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396">
        <property type="entry name" value="NEDD4 FAMILY INTERACTING PROTEIN 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396:SF3">
        <property type="entry name" value="NEDD4 FAMILY-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10176">
        <property type="entry name" value="NEDD4_Bsd2"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="117"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="222" mass="25212" checksum="E9A84C6102AF7C4F" modified="2018-12-05" version="1">MKEPGNKKLYCCVLLFQQLVNEEEPAQPSQESSGQDAPPPYSSVAAANAAFFEYKEEGGIFPNPPPYNVATTLPSYDEAERSKAEAAVPLVTGREDDFVARDDFEDSDQLRIGNDGIFMLTFFMAFLFNWIGFFLSFCLTTSAAGRYGAISGFGLSLIKWVLIVRFSTYFPGYFDGQYWLWWVFLALGFMLFIRGFVNYSRVRKLVDPAYANLPRTRVLFIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLK7</accession>
    <name>A0A3B5LLK7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GOLPH3/VPS74 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLK7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011585"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011717.1">
        <property type="protein sequence ID" value="ENSXCOP00000011585.1"/>
        <property type="gene ID" value="ENSXCOG00000008745.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007153"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048194">
        <property type="term" value="P:Golgi vesicle budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3630.10:FF:000001">
        <property type="entry name" value="Golgi phosphoprotein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3630.10">
        <property type="entry name" value="yeast vps74-n-term truncation variant domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008628">
        <property type="entry name" value="GPP34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038261">
        <property type="entry name" value="GPP34-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704:SF3">
        <property type="entry name" value="GOLGI PHOSPHOPROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704">
        <property type="entry name" value="TRANS-GOLGI PROTEIN GMX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05719">
        <property type="entry name" value="GPP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="281" mass="32533" checksum="1053C6A6CFEDB98D" modified="2018-12-05" version="1">MSVWVIIPPLVTDHTHMHTHTSCWWPFVVCHVRFCHIKIANHDVFHLHFVCKTSSFFFGYTSFWNDCISSGLRACMLIELALRGRLQLEPCGVRRRGLLSRKVLCKSDAPTGDVLLDEALKHIKDTQPPETVQSWIELLSGETWNPLKLHYQLRNVRERLAKNLVEKGVLTTEKQNFLLFDMTTHPLTNNNIKQRLIKKVQEAVLDKWVNDPHRMDKRLVALIFLAHSSDVLENAFAPLLDDQYDLAMKRVRQLLELEPEAESMKANTNEMLWAVVAAFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPD9</accession>
    <name>A0A3B5LPD9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Nectin cell adhesion molecule 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Cell adhesion molecule that promotes cell-cell contacts and plays important roles in the development of the nervous system. Acts by forming homophilic or heterophilic trans-dimers.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Cis- and trans-homodimer. Can form trans-heterodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nectin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPD9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011311"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011442.1">
        <property type="protein sequence ID" value="ENSXCOP00000011311.1"/>
        <property type="gene ID" value="ENSXCOG00000008532.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156933"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902414">
        <property type="term" value="P:protein localization to cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000268">
        <property type="entry name" value="Nectin cell adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000304">
        <property type="entry name" value="Nectin cell adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051427">
        <property type="entry name" value="Nectin/Nectin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277:SF69">
        <property type="entry name" value="NECTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23277">
        <property type="entry name" value="NECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="26"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="163"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="263"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="437"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="518" mass="57188" checksum="BB4B3C0A3BC87E9B" modified="2018-12-05" version="1">MYLLLTEELVLGLGLLLIVINFFSSPLFLSFSLSLCTAVYGQSVQMDPSKSGFVGTQVELRCIFVNSNPPVKISQVTWQKLSNGTKQNVATANPTLGVSILSPFKERVGFKQAAALQRHQMEDTTIVFSNLQLSDEAAYICEYTTFPAGNRENMVNLTVFARPMTRMTLTSPTIVARGKKRNMTVATCTSANGKPPSTIKWNTLLKGEATLQETRNPNGTVTVRSSYVVVPSRETHKQKLTCIVTYRGERITDSVVLNVQYEPEVKIEGFDGNWYLNRQNVQLTCRADANPPVTIYQWKLLNGSLPSNVEIKNNTLFFKGPVTYDLAGTYVCVAINGIGTRTGVVDVNITDLPINPTPRGNGIPPEPHNAGAAIGGAVGGVVLVIVAAILLFVFLRRRQRTFKGDYSTKKHVFGNGYSKAGAMPAHPPIPKNLQYPDDSHDEKKPTQISNTGGFEASERNFDTESEDLKRPYFTVDEGESRDYGERTLAFQYDPEPEIADDMISQTDGSVISKKEWYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQ13</accession>
    <name>A0A3B5LQ13_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Stem cell protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027888538.1">
        <property type="nucleotide sequence ID" value="XM_028032737.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027888539.1">
        <property type="nucleotide sequence ID" value="XM_028032738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQ13"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012865"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013022.1">
        <property type="protein sequence ID" value="ENSXCOP00000012865.1"/>
        <property type="gene ID" value="ENSXCOG00000009728.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114154006"/>
    <dbReference type="KEGG" id="xco:114154006"/>
    <dbReference type="CTD" id="6887"/>
    <dbReference type="GeneTree" id="ENSGT00940000160867"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000015">
        <property type="entry name" value="T-cell acute lymphocytic leukemia 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040238">
        <property type="entry name" value="TAL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13864:SF25">
        <property type="entry name" value="PROTEIN LYL-1-LIKE ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13864">
        <property type="entry name" value="T-CELL ACUTE LYMPHOCYTIC LEUKEMIA/STEM CELL LEUKEMIA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="2"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="85"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00075195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="112" mass="12801" checksum="2EE1780DA6CD24E2" modified="2018-12-05" version="1">MTRKVFTNTRERWRQHNVNTAFAELRKLIPTHPPEKKLSKNEILRLAMRYINFLVQLLESQSGQPAGHSSTALLSFLRGNMERLHSPPRTWTATSDTEAPSPGSRSDSSEAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQ30</accession>
    <name>A0A3B5LQ30_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQ30"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011526"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011658.1">
        <property type="protein sequence ID" value="ENSXCOP00000011526.1"/>
        <property type="gene ID" value="ENSXCOG00000008704.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154973"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19521">
        <property type="entry name" value="RecA-like_VPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000043">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000015">
        <property type="entry name" value="vacuolar protein sorting-associated protein 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF72">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="123"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="40"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="401" mass="44364" checksum="F237730EDC68DD10" modified="2018-12-05" version="1">IVILCVVAADEAQGDRAKQSIRAKCADYLDRAEQLKVYLKQKEKSPPAKPVKESGDKGLDESDEGEDQEKKKFKNQLSGAIVMEKPNVKWNDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSVSSSDLVSKWLGESEKLVKNLFALAREHKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGNDNDGILVLGATNIPWTLDSAIRRRFEKRIYIPLPEEHARSFMFKLHLGSTPNGLTDDNFVTLGKKTDGYSGADISIIVRDALMQPVRRVQSATHFKKVRGSPFSNPDVVVDDLLTPCSPGDPDAVEMTWMEVPGEKLLEPVVSMADMLRSLSNTKPTVNEQDLDKLKKFTEDFGQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRG9</accession>
    <name>A0A3B5LRG9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Low molecular weight phosphotyrosine protein phosphatase</fullName>
            <shortName evidence="11">LMW-PTP</shortName>
            <shortName evidence="11">LMW-PTPase</shortName>
            <ecNumber evidence="3 11">3.1.3.2</ecNumber>
            <ecNumber evidence="4 11">3.1.3.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">Low molecular weight cytosolic acid phosphatase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the low molecular weight phosphotyrosine protein phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="3 11"/>
    <dbReference type="EC" id="3.1.3.48" evidence="4 11"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRG9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013400"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013564.1">
        <property type="protein sequence ID" value="ENSXCOP00000013400.1"/>
        <property type="gene ID" value="ENSXCOG00000010156.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162690"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004726">
        <property type="term" value="F:non-membrane spanning protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048793">
        <property type="term" value="P:pronephros development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd16343">
        <property type="entry name" value="LMWPTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000105">
        <property type="entry name" value="Low molecular weight phosphotyrosine protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050438">
        <property type="entry name" value="LMW_PTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023485">
        <property type="entry name" value="Ptyr_pPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002115">
        <property type="entry name" value="Tyr_Pase_low_mol_wt_mml"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017867">
        <property type="entry name" value="Tyr_phospatase_low_mol_wt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11717:SF7">
        <property type="entry name" value="LOW MOLECULAR WEIGHT PHOSPHOTYROSINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11717">
        <property type="entry name" value="LOW MOLECULAR WEIGHT PROTEIN TYROSINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01451">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00719">
        <property type="entry name" value="LMWPTPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00720">
        <property type="entry name" value="MAMMALPTPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00226">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="Phosphotyrosine protein phosphatases I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphotyrosine protein phosphatase I" evidence="12">
        <location>
            <begin position="7"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR617867-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="155" mass="17226" checksum="71BDE0570A908A6C" modified="2018-12-05" version="1">MAATGSKSVLFVCLGNICRSPIAEAVFRKMATDSGVWVIDSGATSDWNTGSSPDTRGLACLRKHGIETSHRARQVTKDDFMTFEYILCMDESNLSDLNKKAKSVKTSTAKIELLGSYDPQNQRIIKDPYYGSDADFENVYEQCLRCCKAFLEKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRT5</accession>
    <name>A0A3B5LRT5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dishevelled associated activator of morphogenesis 1b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <begin position="1002"/>
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            <begin position="1012"/>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRV1</accession>
    <name>A0A3B5LRV1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Calcitonin peptide-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calcitonin family.</text>
    </comment>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0027">Amidation</keyword>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
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    </feature>
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            <begin position="68"/>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014798.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="117" mass="13220" checksum="462A1CD085767BA0" modified="2018-12-05" version="1">MCTSLLFHTFRIIMFTNHNLSACLYNIIQSCFVNACSQRSISACYCPVSLCMFLSISNSWNRPVSKRCTGLSTCVLGRLSQDIHKLQTYPRTDVGAGTPGKRRSLSELYENYISTYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSP5</accession>
    <name>A0A3B5LSP5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Adenylate cyclase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSP5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013835"/>
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        <property type="protein sequence ID" value="ENSXCOP00000013835.1"/>
        <property type="gene ID" value="ENSXCOG00000010475.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="AC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF7">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16214">
        <property type="entry name" value="AC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="186"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="413"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Adenylate cyclase N-terminal" evidence="4">
        <location>
            <begin position="50"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="440" mass="50266" checksum="46BF3FB8686A394A" modified="2018-12-05" version="1">RLEHPKGADAPCEMLAADRFADPPQPAAAGHLAVGHRLCGVSGLTMSCWRGDTHGVMPEVNVSLVACCVSVMRVFKSKKFQSEKLERLYQRYFFRLNQSSLTMLMGVLVLVFTVMLAFHCSGAPTVTYVVVFSLAIFLTLVLMVVCNRNAFHQDHMWVVCYAVILLVIVIQVIGVLLVQPRSASEGIWWTVFFIHVIYTLLPVRMRAAVVTGVILSAIHVAISWMLNGVDSFLWKQVLSNILVFSCSNIVGVCTHYPAEGSQRQAFQETRECIQARLHSQRENQQQERLLLSVLPRHVAMEMKADINAKQEDMMFHKIYIQKHDNVRNLPHEPNLKFVLDSASIETVLHFAFSSFALILFRIKVINCQMQSSNAQLRMCCVGNFLFCTINVFPEKYMCKTYCFHFIWWEMDSFIWPVLIGWFLFIYSPLMFIFNVSVKPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LT86</accession>
    <name>A0A3B5LT86_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000014322"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014506.1">
        <property type="protein sequence ID" value="ENSXCOP00000014322.1"/>
        <property type="gene ID" value="ENSXCOG00000010853.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156447"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22541">
        <property type="entry name" value="SP5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058018">
        <property type="entry name" value="AAA_lid_TANC1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058056">
        <property type="entry name" value="WH_TANC1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25520">
        <property type="entry name" value="AAA_lid_TANC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25521">
        <property type="entry name" value="WHD_TANC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TANC1/2-like AAA+ ATPase lid" evidence="6">
        <location>
            <begin position="510"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="TANC1/2-like winged helix" evidence="7">
        <location>
            <begin position="607"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="831"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="949"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="982"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="1015"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="1048"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="1081"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="323"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1326"/>
            <end position="1353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1369"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1441"/>
            <end position="1471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="327"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1326"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1369"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1389"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1406"/>
            <end position="1416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1447"/>
            <end position="1457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1862" mass="204858" checksum="B603A6AC0298AAEB" modified="2018-12-05" version="1">SCPYLIVRGHRSAPLLLTMDPAEELGPPPSVDEAADALRTRLGFLLGEKVTVGESCLPYHSPNDGQGNNKGKQASLYHASITSPSSTLESRDSGIIAMLTSYSADSAVEREDGQPVVASTLSSSARSTSHTNGSSMYRIEESLYASTYSLNKLHPNRGFSCSRSSGSTHSIALNLIPRPNSVAATSSAHLEDLAFLDDQQRHLASKMSLKMAGENSGNRNQQDPGVDFTPALNLKPLHFEVPGLLSDWLFMGREWLFQEVDTHLHGNDPTTNRGILITGNMGYGKTAIIARLVALSCHGHHMWPSTTGKQIIPKYGESVLLSTESSGRGGGAGEENGGGSCPGTPEKKRRQQEALWRLAGQVVAFHFCQADNCYTCLVPEFVHNMAAMLITAPQLTAYRKLLQHSPQLQSILSLRSCIQDPSLALQRGILDPLDALHKERKLQIDVEGLIVLIDGLNEAEFHRPDYGDTLTSFLSRNIQKFPLWLKVISTVRTSQQDIAQCLPFHHISLDGKEENNAIDQDLEDYLMERIHSSPGIQSNVSLGNGRLDNTALTKMISHLQSLSKGSYLYLKLTLDLIEGGYLVLKSSSFKVVPVNLAEVYLLQLNMRFPTQSSFQRVLPLLNVTVSSLHPLTDQQLFEVVNAGCLSGETFQWGEFTHRLDQLSSFLLQRSDGSRMLNHASFREWLVWREEGQDDKFLCDPRSGHTLLAFWFCRQEGKLSRQQTLQLGHHILKAHIYKGLSKKLGVSSSILQGLWMSYSTGRLCPVLSSLRNLYTPNIKVSRLLIMGGANVDYRTEVLNGAPLLCVHAHLGHKDVVALLLDHRAQVDAQSHDGLTALGFAAAAGHLDIATLLSHHKAKIGHMDSSGRCVLVLAAQHGHLEVLRFLLKRPDWSCTSCCSQKGTSRSQAVQQALIAAANMGHAEMVSYLLDLPEEDEKEEQRPEINTYDCLWGETALTAAAGHGKIAVCRLLLDQGAAAEQGNRHGITPLFSAVRRGHTRVVEMLLNYGVEVNSVDQQGRTALMTAASEGHIISAQLLLDHGASLNQIDREGLTALSWACLKGQFTLVRELVERGAATDHADRSSRTPLDLAAFCGDPEVVQYLVDHGALVEHMDCSGMRPLDRAVGCRNTSAVIALLKKGAKIGPATWAMATSKPDILMVLLSKMIQEGDRLYKQGKVREAAQSYQSALHKFPRDELKTFKQLRVCVLLNLSRCHRKMNDFSLAEEFATKALELKSNSYEAFYARARAKRSRRQFHAALEDLMDASRLCPSNREIQRLLSRVKEECRQVSQQQDPPSAYQHQMAVSTNEARCRDFNCLHVQNMESFNREDKEVEKEKDGDVREDEDFLSSHPPHTVYSLDTHCYPGLKSPSSLSPTHMHQCLSNPTHLADLSSPSHSLLPPTSPSQPHVSETPSSPLQHPQRAGSMFESRGQHHLQTVSVLSPADHNQGRQQNNLGSDQKSLLKRSPSKSLGLQNTKAEVVLSSQPSFSDHSDLVLGSSAYSQFTNLPEELAELWEDFYPRPFDVRHGPLVRGGVCSRVTHVLDNVGVSPVCQTQATPVHGRGTAADRMVVNDFSQSRQFSRNQSKAAYYPMEVTEAAMGNKSLQDFHYKHQGGVHLPLNAHPTSTFTPPPRSIIPVNVRFSSNIASGLLTNEGQGFRTSASVQHMDLPSESMGDAYGCHDDPFLNSSLQSEICMTGGGTYPGEAGRSSRNTPFMGITDKSVRVHQHYQQQVPSSSASCLSLSRSWAVSSVDTIVTSPSKSSANQRGLAPEALISNAYYNCSNNNAHNSHLHLTQVHLDLCEVAPGNSRWNEDSGHVSSQNPSYLDVKVAQTLPVCQNCSDRSVERIVPTSPVKPKKPFVESNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUA2</accession>
    <name>A0A3B5LUA2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SCD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SCC3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUA2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014717"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014903.1">
        <property type="protein sequence ID" value="ENSXCOP00000014717.1"/>
        <property type="gene ID" value="ENSXCOG00000011176.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182972"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039662">
        <property type="entry name" value="Cohesin_Scc3/SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020839">
        <property type="entry name" value="SCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199:SF3">
        <property type="entry name" value="COHESIN SUBUNIT SA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199">
        <property type="entry name" value="STROMAL ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21581">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51425">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCD" evidence="2">
        <location>
            <begin position="28"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="174" mass="20186" checksum="19CBD0A2A6C66A62" modified="2018-12-05" version="1">MVCFNPSSYRCLGFPFDLLLFTLKKKNSSVLFQDSIAEIRAICIEEIGVWMKLYSDAFLNDSYLKYVGWTMHDKQGEVRLKCLTALQGLYHNRELNARLELFTSRFKDRIVSMTLDKEYDVAVQAIKLLTLVLNTDEVLTPEDCESVYHLVYSAHRPVAVAAGEFLYKKYVRTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUU4</accession>
    <name>A0A3B5LUU4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SUEL-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EVA1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUU4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015758"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015952.1">
        <property type="protein sequence ID" value="ENSXCOP00000015758.1"/>
        <property type="gene ID" value="ENSXCOG00000011908.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163305"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22828">
        <property type="entry name" value="Gal_Rha_Lectin_EVA1_EVA1C_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22829">
        <property type="entry name" value="Gal_Rha_Lectin_EVA1_EVA1C_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.740:FF:000001">
        <property type="entry name" value="Adhesion G protein-coupled receptor L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.740:FF:000003">
        <property type="entry name" value="Protein eva-1 homolog C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.740">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039500">
        <property type="entry name" value="EVA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46780">
        <property type="entry name" value="PROTEIN EVA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14851">
        <property type="entry name" value="FAM176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0348">Hemagglutinin</keyword>
    <keyword evidence="5" id="KW-0430">Lectin</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="252"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="11">
        <location>
            <begin position="18"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="11">
        <location>
            <begin position="118"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="294"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="303"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="372" mass="41469" checksum="709063C4A5167742" modified="2018-12-05" version="1">MSDVRLLKMIITSHSAHACDGTPLRLHCPRHSTISIQSAFYGSGEVRLCTEDPTSGPRNHRCSALTALQKILSECQNLRDCQLPVSHLLFGKDPCPGKNKYLHVNYKCKPTEHKTQVVCEGETIILHCKTPNVLIIYAAVYGRGLGQTDTCPSLSARPPPFECLNYDAVQVVSKSCYSKQKCTIAVGNQTFRDPCFPGTRKYLSVLYSCVPKSLLKEADPNILSSTSLPTTEKDCVLNLLLFIFSHIDHPEMAALLFTSSVCVGLLFTLLALSVQVTCRRRRLRDHTLGAKSNGLTPNCSKQDDEEDEAEDDEDDKEETESSLLSTAERKGIKGWEEVTYVSEEAELAERIERRDMIIQEIWMNSYLNGSSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LX80</accession>
    <name>A0A3B5LX80_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LX80"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012585.1">
        <property type="protein sequence ID" value="ENSXCOP00000012434.1"/>
        <property type="gene ID" value="ENSXCOG00000009395.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160308"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008349">
        <property type="term" value="F:MAP kinase kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000040">
        <property type="entry name" value="Dual specificity mitogen-activated protein kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF15">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="20"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="178" mass="20262" checksum="66B3EDBB4AE12193" modified="2018-12-05" version="1">MDFHQRAALDISTKNPQDDFEILLRVGGGTYGEVYKARNKQNGELAAIKVIKMEPEDDFSIIQQEIVIVKSCKHPNIVAYYGSYIRANKLWICMEFCGGGSLQDIYHVTGSLPEPQIAYICREMLQVSALFSRSLCIFLRTFKVKVSCVCVSRAWTICTHRRKSTEISRSASRNRDKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXR4</accession>
    <name>A0A3B5LXR4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXR4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012614"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012766.1">
        <property type="protein sequence ID" value="ENSXCOP00000012614.1"/>
        <property type="gene ID" value="ENSXCOG00000009536.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166909"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098831">
        <property type="term" value="C:presynaptic active zone cytoplasmic component"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019992">
        <property type="term" value="F:diacylglycerol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017075">
        <property type="term" value="F:syntaxin-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061789">
        <property type="term" value="P:dense core granule priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099525">
        <property type="term" value="P:presynaptic dense core vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035249">
        <property type="term" value="P:synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016081">
        <property type="term" value="P:synaptic vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016082">
        <property type="term" value="P:synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08394">
        <property type="entry name" value="C2A_Munc13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000031">
        <property type="entry name" value="Protein unc-13 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027080">
        <property type="entry name" value="Unc-13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10480">
        <property type="entry name" value="PROTEIN UNC-13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10480:SF1">
        <property type="entry name" value="PROTEIN UNC-13 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="357" mass="40686" checksum="2F0F3A409F2E7AC7" modified="2018-12-05" version="1">FFFNFYTVKKGKLDGPQEKFNTYVTLKVQNVKSTTIAVRGIFLFHGLGLTVEVWNKGLIWDTMVGTVWIPLQSIRQSNEEGPGQWLTLDSNVIMAENEICGTKDPTFHRLLLDTRFELPLDIPEEEARYWAKKLEQLNAMRDQDYAYQEEQEQSLHAPSTQCYEDPDSAVDDRDSDYRSETSNSIPPPFYTTSQPNASMHQYPIFLRLIIPNSNTFFLNQRIPPTDSRYASSAELSRGSSQLSEEFPPEDGESFQGSEFYDENDSYHSCHSSVSYGKEDSPGWDGEDHQGVYSDEGGYLKDGGEEGALYDEEEGDIYAEEGQEIMEPGPARAKANWLRLFSRVRLQLQEVQTAHVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZE6</accession>
    <name>A0A3B5LZE6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Iron-sulfur cluster co-chaperone protein HscB</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a co-chaperone in iron-sulfur cluster assembly in mitochondria. Required for incorporation of iron-sulfur clusters into SDHB, the iron-sulfur protein subunit of succinate dehydrogenase that is involved in complex II of the mitochondrial electron transport chain. Recruited to SDHB by interaction with SDHAF1 which first binds SDHB and then recruits the iron-sulfur transfer complex formed by HSC20, HSPA9 and ISCU through direct binding to HSC20. Plays an essential role in hematopoiesis.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Acts as a co-chaperone in iron-sulfur cluster assembly in the cytoplasm. Also mediates complex formation between components of the cytosolic iron-sulfur biogenesis pathway and the CIA targeting complex composed of CIAO1, DIPK1B/FAM69B and MMS19 by binding directly to the scaffold protein ISCU and to CIAO1. This facilitates iron-sulfur cluster insertion into a number of cytoplasmic and nuclear proteins including POLD1, ELP3, DPYD and PPAT.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; iron-sulfur cluster biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. Interacts with ISCU (cytoplasmic form); this interaction stabilizes the (Fe-S) clusters on ISCU. Interacts with the CIA complex member CIAO1 (via LYR motif).</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with ISCU and HSPA9 to form an iron-sulfur transfer complex. Interacts with SDHAF1 (via the first LYR motif); the interaction recruits the iron-sulfur transfer complex composed of HSC20, HSPA9 and ISCU and mediates the incorporation of iron-sulfur clusters into SDHB which also interacts with HSC20. Interacts with the cytoplasmic form of ISCU and with CIA complex member CIAO1 (via LYR motif).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HscB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZE6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013134"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013295.1">
        <property type="protein sequence ID" value="ENSXCOP00000013134.1"/>
        <property type="gene ID" value="ENSXCOG00000009955.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008206"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051259">
        <property type="term" value="P:protein complex oligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.20:FF:000002">
        <property type="entry name" value="HscB mitochondrial iron-sulfur cluster co-chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000042">
        <property type="entry name" value="Iron-sulfur cluster co-chaperone protein HscB, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.20">
        <property type="entry name" value="HscB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004640">
        <property type="entry name" value="HscB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036386">
        <property type="entry name" value="HscB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009073">
        <property type="entry name" value="HscB_oligo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00714">
        <property type="entry name" value="hscB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14021">
        <property type="entry name" value="IRON-SULFUR CLUSTER CO-CHAPERONE PROTEIN HSCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14021:SF15">
        <property type="entry name" value="IRON-SULFUR CLUSTER CO-CHAPERONE PROTEIN HSCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07743">
        <property type="entry name" value="HSCB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47144">
        <property type="entry name" value="HSC20 (HSCB), C-terminal oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="14">
        <location>
            <begin position="70"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="234" mass="26733" checksum="B57784FFB1429453" modified="2018-12-05" version="1">MSTFHKKDTGHRDANLGGPGYRTNKVFSSRSYSTSEVKLNCWNCKQLLDTTPSFFCDSCKAIQPPEEGTSYFRILDCDYTFTLDTHKLQKRYLQLQRTLHPDNFSQKSVKEQEYSESQSALVSKAYKTLLKPLSRGLYMLELEGMHLKEGTDSVAESHFLMELMEINEALEEAQTPEEVNKIGQDTKGKLADLTELIDTALHEGKLDTAKELLTQMKYYASIEEKVKAKLSDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M367</accession>
    <name>A0A3B5M367_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Developing brain homeobox 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the H2.0 homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027869773.1">
        <property type="nucleotide sequence ID" value="XM_028013972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M367"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018827.1">
        <property type="protein sequence ID" value="ENSXCOP00000018593.1"/>
        <property type="gene ID" value="ENSXCOG00000013992.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114142610"/>
    <dbReference type="CTD" id="30394"/>
    <dbReference type="GeneTree" id="ENSGT00950000183093"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021515">
        <property type="term" value="P:cell differentiation in spinal cord"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000177">
        <property type="entry name" value="Homeobox protein DBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051662">
        <property type="entry name" value="H2.0_Homeobox_NeuralPatt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24331">
        <property type="entry name" value="DBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24331:SF6">
        <property type="entry name" value="HOMEOBOX PROTEIN DBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="157"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="159"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="218"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="270"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="289"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="299" mass="32868" checksum="4E2F60B7ED38AEFF" modified="2018-12-05" version="1">MMIPSVLAPPALYPGLYRPAAALPSAFPAHSSFLVEDLLRISRPTAFITRTAPPVGASLPAASYNGASAVTRDSCSPKTSKDPSFLKFGVSAILAPSPKTASSPPAIHSLHSKTFPVPCFDGTFHPIFRTPYLPATSSVVPIPGTFSWPLAARGKPRRGMLRRAVFSDVQRKALEKMFQKQKYISKPDRKKLASKLGLKDSQVKIWFQNRRMKWRNSKERELLSSGGCREQTLPTKSNPHPDLSDVGKKSSAEDEEEEEEDEEFRRERMSAAGCSISSPSMSSKHSDFSESDDEEITVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M404</accession>
    <name>A0A3B5M404_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M404"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016156"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016362.1">
        <property type="protein sequence ID" value="ENSXCOP00000016156.1"/>
        <property type="gene ID" value="ENSXCOG00000012209.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271823"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243:SF207">
        <property type="entry name" value="PYROKININ-1 RECEPTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="242"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="289"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="1"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="365" mass="42683" checksum="8D99CA96CD4B5584" modified="2018-12-05" version="1">MLLLHLNVPSNKGYFYSNIPILIFFWYFALIKNFLSILIVTDILQLLTIPVTLYWYYWESYPWRLGQAVCKVYFMVRQIYCATTSWVIVTFTTERYAAICHTMWSVSSLKKSRLPCLVTVWIVSLVSAVPFALVYDQARACILDYTATTQEDAFKVSTMCEMTEPDPAHIYKGALLLRAGLFFLVPLVSIFTLYMFILLHLRKNGRQRRNMGLMRPDLEGRRDIQCHRNGKLLLNEKRALKLMGAVVVSFFLFNFPDIASSLMQVYVVVWSDRVLSIYTVLKSYLSLPLWYINGALDPLLFCISSHTFRRACWRTLRRLRPRCFLRYRGLSWLRTQSSSEDASTMAKEKIDRKFCKAKSDKKEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4L8</accession>
    <name>A0A3B5M4L8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DEAD (Asp-Glu-Ala-Asp) box polypeptide 28</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4L8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016366"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016572.1">
        <property type="protein sequence ID" value="ENSXCOP00000016366.1"/>
        <property type="gene ID" value="ENSXCOG00000012364.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161738"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="178"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="395"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="564" mass="62275" checksum="34C5050304F5F8B0" modified="2018-12-05" version="1">NMFVKEGALKALGSGRLFRSELLTASLSHHFSATQAHFCQTEPNPEDTVIIRVPRYLLKRVENVKQARGKPKISTVKAGKLLIQSRNPSLNQSAGFTAGKFEQPVLCSKGWKHNKSFGDYFTINNIKEVAPFIAASQSGDTEQKSPTTFQNLHICKELVETLERVEITHPTIVQLQTIPKVMRGHNILCAAETGSGKTLSYLLPVVHRLQENTQDKTTDRIRAVVVVPSRELAEQVAAVSRTLCMPLGLRTSTVGGGRGVGHIKTVFKRDHPDILVATPGALVKGLRRNCLDLSELNFLVVDEADTMFDPSFSDLLSEILSHTNIASNPKETRGLGHKTQLLLIGATFPDGVGEVLSKVTDLGKIITIKSKMLHYLMPHVKQTFLKVKGADKVLELYQYLTKLQHEKGGGAALVFCNKSSTVNWLGYSLEEMKVQHARLQGEMPAVVRTGIFHSFQKGAVNVLICTDIASRGLDTSRVRLVVNYDFPESHTDYIHRAGRVGRAGGVEDGEVLSFVTHPWDVELVQNIETAARRKTSLPGMKSEIQEPKAKVENTEQMIHMLGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7S1</accession>
    <name>A0A3B5M7S1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7S1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015834"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016030.1">
        <property type="protein sequence ID" value="ENSXCOP00000015834.1"/>
        <property type="gene ID" value="ENSXCOG00000011958.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182937"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000106">
        <property type="entry name" value="serine/threonine-protein kinase TBK1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051180">
        <property type="entry name" value="IKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22969">
        <property type="entry name" value="IKB KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22969:SF14">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE TBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="9"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="134" mass="15226" checksum="C184B21E78D16D74" modified="2018-12-05" version="1">MQSTTNYLWLISDLLGQGATANVYRGRHKKTGDLYAVKVFNNLSFLRPLDVQMREFEVLKKLNHKNIVKLFAVEEETNARHKVLVMEYCPCGSLYTEMEGVMKELAENNHFLERSASPCVTGSVWVRTGSGPDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8N1</accession>
    <name>A0A3B5M8N1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DAGKc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M8N1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019892"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020139.1">
        <property type="protein sequence ID" value="ENSXCOP00000019892.1"/>
        <property type="gene ID" value="ENSXCOG00000014939.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156976"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001729">
        <property type="term" value="F:ceramide kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006672">
        <property type="term" value="P:ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045363">
        <property type="entry name" value="CERK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057465">
        <property type="entry name" value="CERK_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF25">
        <property type="entry name" value="CERAMIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19280">
        <property type="entry name" value="CERK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25382">
        <property type="entry name" value="PH_CERK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAGKc" evidence="1">
        <location>
            <begin position="101"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="466" mass="52306" checksum="8429DEDC76AECAF9" modified="2018-12-05" version="1">MNLCTCNFSGSYQAAVTIKLNCQFNLSSEHRQTVEVCEIVAVHKTEDEVDKLFLLSFAVSYVRRTRQHLWRCADVTFHCADQQICEQWIQVTNEQLSILTNRPKNLLVYINPYGGKRRGERIYEQKVVPVFRRAGIFTDVIVTERANHARDHLKTEANLDDYDGVVCVGGDGMFSEILHGLVTRIQTDHGVDQNQPDAELVPCSLRIGIIPAGSTDCICFATQGTNDPVTSALHIVVGDSQPMDVCSVHHNGVFLKYSVSLLGYGFYGDVLADSERNRWLGPARYDLSGFKTFLSHNHYGGTVSFLPADDNVGHPRDKLCAPVSSDSDRGWSVINGKFIAINSASMSCACPRSPKGLSPSAHLADGTTDLILVRKCSRLDFFKHLLRHTNNNDQFDHWFVEVHRVRKFRFQPWHHNFVPLEDASERPESPDLSPISSLQETCDDCTAESSWNCDGEILHHTAIQVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9N6</accession>
    <name>A0A3B5M9N6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9N6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016686.1">
        <property type="protein sequence ID" value="ENSXCOP00000016479.1"/>
        <property type="gene ID" value="ENSXCOG00000012437.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244874"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF528">
        <property type="entry name" value="C-FAMILY ODORANT RECEPTOR OLFCD2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017386440" description="G-protein coupled receptors family 3 profile domain-containing protein" evidence="13">
        <location>
            <begin position="18"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="574"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="608"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="641"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="685"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="730"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="765"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="797"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="14">
        <location>
            <begin position="571"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="847" mass="94823" checksum="91247BF9C3555A8E" modified="2018-12-05" version="1">MGLIIILFLTLLARTKPENQTCKVIEKAELMEFSRDGDLNIAGVFSITSTRTLVDNDYQAIPYSYCKGLVNLKFARTMIFTVEEINRDPSLLPGLRLGYKVYNGCGTENLIRAAIEAINGNDPLACGGQTLVVLGHSSSGVSSDINTIISAVSVPQISHLSTCACLSDKARYPTFFRTVPSDKFQITALVQLMKYFDWRWVGIIHSVGTYAEEGASEFVKEAQKEGVCVEYTMVYLETSRSRLRTIIETLKESSSKVVLMFMPLSFAKTFLYHMEEYNITGKQWVGSESWISAKYLASAERKHILHGAIGYAIPQASIPGLGEFLLSLKPSDEPHGALIKNIWEEFFDCSFTPSNTSTLCTGAEDLRTVLSDYTDVAKFREENNVYTAVYSVAFALHLLMQCENGLNPTMGNPCLTKDERINFTTKNGARVFFDENGDSIAKYDLVNWQMKEDGSADIFIIGQFDNSSPEGKKLKLKEYAKIVWGASCILFKCNQVVRSVCREPCPPGTRKAVNKNKPVCCFDCFQCSEGTISNKTNSLDCSPCPPEVWPNEMRDNCVPKPTEYLSYTETTGTLLTGFGTFGVFLSLLITTIFFTHKETPIVRANNSELSFLLLFSLKLCFLCSLTFIGRPTQWSCMLRHTAFSITFVLCISCVLGKTIVVLMAFSARRPGSNMTKWFGPVQQRVSVLILTLVQVVICIIWLTINPPFPKRNMQYYKDRIILECALGSAIGFWAVLAYIGILATLCFVFAFLARKLPDSFNEAKLITFSMLIFCAVWITFIPAYFSSPGKFTVAVEIFAILASSFGLLFCIFAPKCYIILLRPEQNSKKRIMGKIRRKKCEAMFECI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDG6</accession>
    <name>A0A3B5MDG6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sister chromatid cohesion protein DCC1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DCC1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027867550.1">
        <property type="nucleotide sequence ID" value="XM_028011749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDG6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021570"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021834.1">
        <property type="protein sequence ID" value="ENSXCOP00000021570.1"/>
        <property type="gene ID" value="ENSXCOG00000016142.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114141253"/>
    <dbReference type="KEGG" id="xco:114141253"/>
    <dbReference type="CTD" id="79075"/>
    <dbReference type="GeneTree" id="ENSGT00390000017400"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034088">
        <property type="term" value="P:maintenance of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019128">
        <property type="entry name" value="Dcc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395:SF6">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09724">
        <property type="entry name" value="Dcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="390" mass="45045" checksum="CB8F10376499509E" modified="2018-12-05" version="1">MRTLEEVQATLEIAKLKEEDLLSTVHCLSFGDNVSSADYCLMELDETLCKHIESGESLVIRGDKDERAVLCSSDKTYDLKIADTSNLLLVVPGCRTPDQLTDNQDSTHVVHTQIWGFCNSYWELRKQRPKLKKLKKLLMENPYEGPAIRGQEDNTENRYTMEDLLERIQASEEELKTHLETIHACRIDGYWRVLDFDYEMKLLGHVTQLVDSESWSFNKVPLHTSLEELAPLEPREMIEHCLNCYGKRYIESGEVFYALHEDRVCWGLALMLLQNAVKFNLKEFQDVWQQSVPEGMTTRLEQLKGVALVDRTSRPETISLLRVEDLPEDTVERFNHLFSLREKWTEDDITPYIQDLCGEKQTTGALLTKYARSSMQSGVKVFNSRRPVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEM8</accession>
    <name>A0A3B5MEM8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hydroxyacid-oxoacid transhydrogenase, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Alcohol dehydrogenase iron-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEM8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022122"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022391.1">
        <property type="protein sequence ID" value="ENSXCOP00000022122.1"/>
        <property type="gene ID" value="ENSXCOG00000016534.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000179003"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496:SF83">
        <property type="entry name" value="HYDROXYACID-OXOACID TRANSHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA" evidence="4">
        <location>
            <begin position="46"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00021046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00032098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="112" mass="12598" checksum="3E182ECB90DA9DA5" modified="2018-12-05" version="1">MAGRDRIFHLLRQLERAACRCPAHSHTFHQAAGDRKTDYAFEMASSNIRYGAGVSAEVGMDLQNLGAQNVCLMTDRTLTRLPPVQAVLESLVRNRVHFQVFDDVRVEPSDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MF93</accession>
    <name>A0A3B5MF93_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 21">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MF93"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022973"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023250.1">
        <property type="protein sequence ID" value="ENSXCOP00000022973.1"/>
        <property type="gene ID" value="ENSXCOG00000017170.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159017"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000363">
        <property type="entry name" value="E3 ubiquitin-protein ligase ZNRF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051878">
        <property type="entry name" value="ZNRF_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46661">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZNRF1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46661:SF3">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZNRF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15" id="KW-0458">Lysosome</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="28"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="71" mass="8062" checksum="303A4066E12EBBCE" modified="2018-12-05" version="1">MDLHLVLCLTKPRVTYNEDVLSKDAGECAICLEELQQGNTIARLPCLCIYHKGCIDEWFEVNRSCPEHPAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFY8</accession>
    <name>A0A3B5MFY8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MFY8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020031"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020278.1">
        <property type="protein sequence ID" value="ENSXCOP00000020031.1"/>
        <property type="gene ID" value="ENSXCOG00000015034.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271828"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF237">
        <property type="entry name" value="MHC CLASS I ANTIGEN TRANSCRIPT VARIANT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00129">
        <property type="entry name" value="MHC_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="176"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="327" mass="37560" checksum="ECA8FB6A9DD1E3E7" modified="2018-12-05" version="1">TRIKLLTFKNSLTFFAIISTGIKNLPNLFTTVVDDIQVDYYDSNGTRKQKTLNSLENLTISYFNKTANLNRTEGTVLLPDIYMLNTLFFKTSHSNFLILSVLAVLQCMSGCELDEKSAEVVTFQKCGYNGEDFMKLDFKNLTWIAQHTLAENFTLELNLDQQRLNNTKVFLSLTCPKVLNEFVIRSALQREDLTSVVLLQKTPSSLVNCFATGFYPNTARMFWMRDGMEIQNDKEPSEILPNHDNTYQMSVYLNVSSIASEDWKRYDCVFRLGNKTKLTRLKKEITEDGKLSSQIAQIKYLFFNLVIKRINKAIQSALQELTGVLYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MG51</accession>
    <name>A0A3B5MG51_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Neural EGFL like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NELL1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MG51"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018579"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018813.1">
        <property type="protein sequence ID" value="ENSXCOP00000018579.1"/>
        <property type="gene ID" value="ENSXCOG00000013986.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00810000125439"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005080">
        <property type="term" value="F:protein kinase C binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045778">
        <property type="term" value="P:positive regulation of ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045667">
        <property type="term" value="P:regulation of osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.200.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024731">
        <property type="entry name" value="NELL2-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051586">
        <property type="entry name" value="PKC-binding_NELL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24042">
        <property type="entry name" value="NEL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24042:SF2">
        <property type="entry name" value="PROTEIN KINASE C-BINDING PROTEIN NELL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12947">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="VWFC" evidence="8">
        <location>
            <begin position="25"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="188"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="230"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="271"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="303"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="8">
        <location>
            <begin position="401"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="273"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="291"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="519" mass="57316" checksum="C633675D47B70D64" modified="2018-12-05" version="1">VISQQIILNYAESRLTQLEGCHCERTCSANGLVYRDKELWVEPENCRNCACKNGVVECRRIFCPPANCSDDMLPVHVDGTCCKTCRPKCTYMGQTFSEGQRFLSRTCRECKNGLMVKVTETCPEINCTTGEQILPEGRCCNVCRGYDFCAEGLICGENSECKNRNTKAECECKSGYASIHGDSTYCEDIDECATQMHYCQSNTMCVNLPGSYRCDCLPGFTRVDEYSCTEHDECASGQSLCNENAICTNTIRGHLCTCKPGYVGNGTICRAFCEDGCRSGGTCVSPNTCVCPSGFTGHRCETDIDECAEGLIECHNHSRCVNLPGWYHCECRSGFHDNGSYQLDGSSCIGEHTELKSPLSFLLVTCIAQDGQTFCRRKPCDCADPDEDLFCCPSCDNRPSSQCLDQSGRTLYRSGASWMYGCQQCRCMEGEVDCWPLVCPVLLCEYTAVAEGECCPRCVTDPCLADNLSYDIRQTCTDPTGVTRLSGDTWHMPKSPCTTCKCKNGNVCCLVDLDCLQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGE7</accession>
    <name>A0A3B5MGE7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Enkurin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
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            <taxon>Cyprinodontiformes</taxon>
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            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGE7"/>
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        <property type="protein sequence ID" value="ENSXCOP00000018669.1"/>
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        <property type="term" value="C:axonemal microtubule"/>
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        <property type="entry name" value="Enkurin_dom"/>
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        <property type="entry name" value="Enkurin_domain-protein"/>
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        <property type="entry name" value="ENKURIN"/>
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        <location>
            <begin position="21"/>
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        <location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51665"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018669.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="267" mass="31009" checksum="4A672ED12CA330BB" modified="2018-12-05" version="1">MNKNILTECLSATITFHRRHIAFRVILFYSFLLLFFSASVIEDILFCDRYVPKHLATILLDQKPNKQAWKTMGPAKLELPSPSNYLKKNAKRPKAPESKFLKQLPPLHVRANYPLSPIKRDVIQTADVKPKPSHVDFAQTHKQLLQSAGLSPKYVMKKDFGQVPMYLQQRRAAEQQARDDLERLEKEKQATRQVSEDERKAALEGLKKKWHEVHHEYQGLPFILYTPSQKARKIHMEEQLTELEKHIRLLEKFKTIYISDADAGGCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGT0</accession>
    <name>A0A3B5MGT0_9TELE</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_028005997.1"/>
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    <dbReference type="STRING" id="32473.ENSXCOP00000018794"/>
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        <property type="protein sequence ID" value="ENSXCOP00000018794.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="entry name" value="Ribosomal_mS37_met"/>
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        <property type="entry name" value="CHCHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31278:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="122" mass="13583" checksum="C7C63FCD554131A2" modified="2018-12-05" version="1">MAAQGGVLFQEKVSRLLSRENGKPVLKSNRPLVLKDSVANRKPKKGEASCITEMSLLMACWKQNNFVDSLCSNEVNTFYTCVHKAEAARKNKSEQSNVTGGRLTPKQATTLLKRFPNLRSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MH01</accession>
    <name>A0A3B5MH01_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP-dependent helicase RENT1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA2/NAM7 helicase family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A3B5MH01"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020391"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020639.1">
        <property type="protein sequence ID" value="ENSXCOP00000020391.1"/>
        <property type="gene ID" value="ENSXCOG00000015275.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:hydrolase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:RNA helicase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903313">
        <property type="term" value="P:positive regulation of mRNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21407">
        <property type="entry name" value="1B_UPF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEXXQc_UPF1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21400">
        <property type="entry name" value="ZBD_UPF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Regulator of nonsense transcripts 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000097">
        <property type="entry name" value="Regulator of nonsense transcripts 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040812">
        <property type="entry name" value="UPF1_1B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018999">
        <property type="entry name" value="UPF1_CH/ZBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF364">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18141">
        <property type="entry name" value="UPF1_1B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09416">
        <property type="entry name" value="UPF1_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51997">
        <property type="entry name" value="UPF1_CH_RICH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Upf1" evidence="16">
        <location>
            <begin position="94"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="C3H" evidence="14">
        <location>
            <begin position="102"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="CC/SHH/C" evidence="14">
        <location>
            <begin position="116"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="C4" evidence="14">
        <location>
            <begin position="162"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="996"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1002"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1026"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1042"/>
            <end position="1052"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1086" mass="120431" checksum="8B661DDB90A4427A" modified="2018-12-05" version="1">MSVEAYGPSSQTLTFLDSEESELLGADTQGSEYDFTDFTLPSQTQTQGQTHGQLDNQVNGSDVVLQNGDDPVGKASQLLAELNFEEDEEDAYYSKDLPLHACCYCGIHDPACVVYCNTSKTWFCNGRGNTSGSHIVNHLVRAKSKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPTATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQENITIRWDLGLNKKRIAYFTLPKTDSDMRLMQGDEICLRYKGDLAPPWKGIGHVIKVPDNYGDEIAIELRSSAGAPVEIPHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVLIKCQLPKRFTVQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGTGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMESMPELQKLQQLKDETGELSSSDEKRYRALRRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGTKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTRLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFNKPRKLVNAINPGGRFMSTAMYDAREALIPGSVYDRSSNGRPSNMYFQTHDQIGMIGSGPGPMSSLNIPIPFNVMMPPIPPPGYLGQLNGPSAGERSVPVNVGIDGGHMTGSQASQDQGSQPFSQGPLTQGYISMSQPSQMSQPGLSQPELSQVSSSTRCLSHGGGSLEIATPSRRRIIACEGGARSTQAFLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHB4</accession>
    <name>A0A3B5MHB4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHB4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020506"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020756.1">
        <property type="protein sequence ID" value="ENSXCOP00000020506.1"/>
        <property type="gene ID" value="ENSXCOG00000015366.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005949"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051:SF0">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35, MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="214" mass="24528" checksum="80F160B3D0F6E166" modified="2018-12-05" version="1">MDWVFVMNRMSSQGSTAAQRRRMALGVVILLLVDVIWVYIFKRQDYNKPFFSTFTKTSMFVLYLLGFLLWRPWRQQCSGALKRRQSAFFADAEAYFAPCTTDSAVNNCLSEPLYVPMKFQDAAADPYDCLPQRVRFSNIMEVRQLPSTQALEAKLSRMSYPAAKDHEALLRTVGKLTITDVAKISFFFCFVVRKAGPPHRGANCSPLMFRALIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJ90</accession>
    <name>A0A3B5MJ90_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trafficking protein particle complex subunit 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TRAPPC13</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the multisubunit TRAPP (transport protein particle) complex.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000023827"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024111.1">
        <property type="protein sequence ID" value="ENSXCOP00000023827.1"/>
        <property type="gene ID" value="ENSXCOG00000017799.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015280"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010378">
        <property type="entry name" value="TRAPPC13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055429">
        <property type="entry name" value="TRAPPC13_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055427">
        <property type="entry name" value="TRAPPC13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13134">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13134:SF3">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23647">
        <property type="entry name" value="TRAPPC13_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06159">
        <property type="entry name" value="TRAPPC13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trafficking protein particle complex subunit 13 N-terminal" evidence="3">
        <location>
            <begin position="10"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Trafficking protein particle complex subunit 13 middle" evidence="4">
        <location>
            <begin position="172"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="373" mass="42000" checksum="37F2232DAB8ED5DB" modified="2018-12-05" version="1">MDVNQAKQEHLLALKVMRLTKPTLFTNLPVTCEDRDLPGDLFAQLMREDPSTIKGAETLMLGEMLTLPQNFGNIFLGETFSSYISVHNDSNQVVKDILVKADLQTSSQRLNLSASNSAVAELKPECCIDDVIHHEVKEIGTHILVCAVSYTSQFGEKLYFRKFFKFQVLKPLDVKTKFYNAETDEVFLEAQIQNITTSPMFMEKVSLEPSMMYNVTELNSVASGDEGESTFGKMSYLQPMDTRQYLYCLKPKPEYAEKAGVIKGVTVIGKLDIVWKTNLGERGRLQTSQLQRMAPGYGDIRLSLEVIPDTVNLEEPFDIVCKITNCRCIRSSITLIITYLIIPVELIKHYSKTVGVSXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKA4</accession>
    <name>A0A3B5MKA4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Carboxypeptidase Q</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Plasma glutamate carboxypeptidase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="20">Homodimer. The monomeric form is inactive while the homodimer is active.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase M28 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKA4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021571"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021835.1">
        <property type="protein sequence ID" value="ENSXCOP00000021571.1"/>
        <property type="gene ID" value="ENSXCOG00000016143.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018110"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070573">
        <property type="term" value="F:metallodipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03883">
        <property type="entry name" value="M28_Pgcp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000036">
        <property type="entry name" value="Carboxypeptidase Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000009">
        <property type="entry name" value="Carboxypeptidase Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039866">
        <property type="entry name" value="CPQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12053:SF3">
        <property type="entry name" value="CARBOXYPEPTIDASE Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12053">
        <property type="entry name" value="PROTEASE FAMILY M28 PLASMA GLUTAMATE CARBOXYPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="19" id="KW-0458">Lysosome</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="11 22" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="17" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017449256" description="Carboxypeptidase Q" evidence="22">
        <location>
            <begin position="19"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="23">
        <location>
            <begin position="256"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00025833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00033328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="459" mass="50726" checksum="D0729BB555E29EE8" modified="2018-12-05" version="1">MFLLLGFVLTLNSRPANSQTPDVAAEMGRYEDVAKRIIELAVFGAAQNRSYRRLADFTDTIGNRVSGSRNLEMAIKYMYTAMKQDGLDVHLEPVKIPHWVRGKESAEMIMPRTKSLAILGLGSSVGTPPEGVEAEVLVVQSFEELKRRASEATGRIVVFNQPFISYSETVAYRAFGASEAAKVGAVATLIRSVTPFSINSPHTGLQIYQDGVKRIPTACITVEDAELMWRLAQNKQKIVVRLIMGAQTLPDADSFNTVAEIRGWQHPEQVVLLSGHLDSWDVGQGAMDDGGGVMISWEALSLIKDLGLRPRRTLRAVLWTGEEQGGVGAQQYFNLHKENISNFDLVMESDLGTFTPMGLQFLGSEAARKVMEEVIKLLAPINVTKLETDAEGTDIFLWMQAGIPGASLHVADSRYFWFHHSEGDNMTVQDPHDMDLCSALWAVVAYVVADLEDMLPRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLQ6</accession>
    <name>A0A3B5MLQ6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLQ6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025339"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025646.1">
        <property type="protein sequence ID" value="ENSXCOP00000025339.1"/>
        <property type="gene ID" value="ENSXCOG00000018923.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="110" mass="12429" checksum="58F916F39D230E90" modified="2018-12-05" version="1">DWKKILLSDGAKFEIFKMVPQCVVLTVKHGGGRVIHHAAPSGMWLIVQGFILQQDIDPKHNTKNDIRKNEEGGKIENIKWQTQSPDLIPIKLVVLQVALSVRRANLNRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNN1</accession>
    <name>A0A3B5MNN1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cilia- and flagella-associated protein 91</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CFAP91 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNN1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025393"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025700.1">
        <property type="protein sequence ID" value="ENSXCOP00000025393.1"/>
        <property type="gene ID" value="ENSXCOG00000018964.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003024"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026720">
        <property type="entry name" value="CFAP91"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032840">
        <property type="entry name" value="CFAP91_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22455">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22455:SF10">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14738">
        <property type="entry name" value="CFAP91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CFAP91" evidence="8">
        <location>
            <begin position="117"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="518"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="649" mass="77279" checksum="C676025BCEA1DD1E" modified="2018-12-05" version="1">PSEPHHHRPRVRAYTTKERFRKLHESDYLLSGEPLLTFKTDPAEPMPSRSDYRWQAHAERLSLCIATVHPSDYWKYYKRPWIPFEEKFPADVVFEFAEEDFITTTKEQLPAKRSLGIQTDFRESETQTDPYSPEYVVQRGMTPTELLQLATLTWGHGLPAGLAEVEMIERAREKRIWETTLPPRHDLTQIDKRRRMMEEMEAKEWAFRESEIEKVQEIRLAVLGELLRQRDEAQKDTAYDRLTQLYAKHLKDRELKLEKTHHRYIRCKTSHRFDENNTLMKAFSRSSHSLLCLQQLENWKLRGKMWRENCSDGTSLLIFRLTMTSLQELETGLAASLKQDYRVKIKLSECIIWHLLPYSLQIVVKQAVRDEEGKEIEVKGHRFPFRKEKPLPTPPVTPLVEIPSEEDEKTELAIIFLQKLLRGRSIQFEGKENQLELIRELRKVHALQAREQNMQKAEKDFILMLKRERDQQMEKAAIKEARQAAVIGVDLAQVFFILSQELIHLQEERRVHAFMLLAERERRRREAEESGRRQVEERRRQERDEIFREIVQVHQESVDMYLEDMILRNVDYLAEQQAREEIHKKAQQLNDIAYAMEEKRWEREETFILCRIGRPLKRRSTLSCQCFHKSPAPVLLQTVKQFHVVRLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNP5</accession>
    <name>A0A3B5MNP5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diamine acetyltransferase 1</fullName>
            <ecNumber evidence="5">2.3.1.57</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Polyamine N-acetyltransferase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Putrescine acetyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Spermidine/spermine N(1)-acetyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SAT1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>an alkane-alpha,omega-diamine + acetyl-CoA = an N-acetylalkane-alpha,omega-diamine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11116"/>
            <dbReference type="Rhea" id="RHEA-COMP:9766"/>
            <dbReference type="Rhea" id="RHEA-COMP:9767"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:70977"/>
            <dbReference type="ChEBI" id="CHEBI:70988"/>
            <dbReference type="EC" id="2.3.1.57"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:11117"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>spermidine + acetyl-CoA = N(1)-acetylspermidine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28150"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:58324"/>
            <dbReference type="EC" id="2.3.1.57"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:28151"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>spermine + acetyl-CoA = N(1)-acetylspermine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33099"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:45725"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58101"/>
            <dbReference type="EC" id="2.3.1.57"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:33100"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amine and polyamine degradation; putrescine degradation; N-acetylputrescine from putrescine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.57" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNP5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021104"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021363.1">
        <property type="protein sequence ID" value="ENSXCOP00000021104.1"/>
        <property type="gene ID" value="ENSXCOG00000015788.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183121"/>
    <dbReference type="UniPathway" id="UPA00188">
        <property type="reaction ID" value="UER00363"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004145">
        <property type="term" value="F:diamine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019809">
        <property type="term" value="F:spermidine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009447">
        <property type="term" value="P:putrescine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032918">
        <property type="term" value="P:spermidine acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000011">
        <property type="entry name" value="Diamine acetyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051016">
        <property type="entry name" value="Diverse_Substrate_AcTransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10545:SF36">
        <property type="entry name" value="DIAMINE ACETYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10545">
        <property type="entry name" value="DIAMINE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="16">
        <location>
            <begin position="4"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="172" mass="19872" checksum="06F8576E8C5F0951" modified="2018-12-05" version="1">KMEYKLRKAEPKDVSDILRLVKELAKYEEMESQVTLTEKDLLEDGFGETPFYHCIIAETPGENSSDEPSVVGFAMYYFTYDPWVGKQLYLEEFYVMDKCRGLGIGSGILRHLSDLAMKTRCTGMMFVVAENNEPSVQFYKRRGAEDLSQEEGWRLFRFDKDSLVKMATPAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MP02</accession>
    <name>A0A3B5MP02_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MP02"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022886"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023163.1">
        <property type="protein sequence ID" value="ENSXCOP00000022886.1"/>
        <property type="gene ID" value="ENSXCOG00000017094.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155721"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="162"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="333" mass="37552" checksum="734DB377D148696A" modified="2018-12-05" version="1">MKSPVHRCRDVEHGRGQAGSGAGSGGGGGSNCLQPEQRIPVSKDVITSSSSSALWFIRDTCGIVCAIITWLLVFYAEFVVLFVMLVPSKNLTYSIVNGTLFNTLAFLALASHLRAMCTDPVCILRWICFCYCFLFSGAVPKGNATKEYIESLQLKPGQVVYKCPKCCSIKPDRAHHCSVCKRCIRKMDHHCPWVNNCVGENNQKYFVLFTMYIALISLHSLAMVVFHFLYCFEDDWTKCSSFSPPATVILLILLCFEGLLFLIFTSVMFGTQVHSICTDETGIEQLKKEERRWAKKTKWMNMRAVFGHHFSLLWLSPFSTPDHGKAETYQYVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPR1</accession>
    <name>A0A3B5MPR1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Protocadherin-8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Calcium-dependent cell-adhesion protein. May play a role in activity-induced synaptic reorganization underlying long term memory. Could be involved in CDH2 internalization through TAOK2/p38 MAPK pathway. In hippocampal neurons, may play a role in the down-regulation of dendritic spines, maybe through its action on CDH2 endocytosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">The N-terminal extracellular domain forms homophilic interactions; these interactions activate p38 MAPK via TAOK2 and trigger endocytosis. Interacts with CDH2; this interaction may lead to CDH2 cointernalization. Interacts with CDH11. Interacts with TAOK2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Presynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MPR1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025516"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025823.1">
        <property type="protein sequence ID" value="ENSXCOP00000025516.1"/>
        <property type="gene ID" value="ENSXCOG00000019066.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165888"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000120">
        <property type="entry name" value="Protocadherin 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000003">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000117">
        <property type="entry name" value="protocadherin-8 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF244">
        <property type="entry name" value="PARAXIAL PROTOCADHERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 21" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 24" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017451346" description="Protocadherin-8" evidence="24">
        <location>
            <begin position="18"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="689"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="25">
        <location>
            <begin position="25"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="25">
        <location>
            <begin position="124"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="25">
        <location>
            <begin position="233"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="25">
        <location>
            <begin position="347"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="25">
        <location>
            <begin position="452"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="25">
        <location>
            <begin position="568"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="806"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="830"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00056898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00064553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00067805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="948" mass="104462" checksum="7272D84F667B1ED1" modified="2018-12-05" version="1">MKLSVLYLLSLLHCCCLTVLYFTYEEDAPGTEIGNLSQDLKIDPSDAHDTSFRFMQESNTSVIHMRESDGLLTVADVIDREQLCPRSPRCFITFNIVAHSREKFQLIHVEIEVKDINDHSPYFPLNETKLEIVENVPLDSRFPLQIALDEDVGDNYIQSYNISPCSHFAVEVRELEDGVKFAELVLVSELDREVEDSYTVQVTASDGGAPPKSGSMTVNIKVLDFNDNSPTFAHSSLKVELEEDSPVGHRVLKVHAFDPDQGINGEVVYAFPDELSSEAAQTFHIDPYSGDVTLKALVDFEKRKSYELIIRASDQGENSVPSTCTVVIEVVDVNDNAPEIIIKPMTSSSDSVAYITEAAAAESFVALISTSDRDSGSNGYVRVSLLGHEHFTLQQAYGETFMIVTTGTLDREKIQEYNLTVVAEDLGTAPFKTARQYTISVTDENDNPPLFSKSVYEVSVLENNIPGSYITTVVARDPDMGKNAKVSYKLLDSEIPGGSPVSTYVSVDSISGSLYTLRSFDYETLQQIEVVIQAEDRGSPSLSSTSMIRIRVVDQNDNYPYFTFPVLLNNSADIPLPYNAPAGYLALQVVAEDADEGVNGELSHQILEGDPKLFTMNKNTGEIALKQWLTAEIGDVLEIKITVSDNGRAPLSSSATIRFAVSDTEPPEDQVVIVLQSSEEEGSSFDGSLIIIIVLSGGCAFLLIAIVAVALTCKLSRGGRRSRGRKREVCQGLFDGRPHSMLSSAEPNIYTGQQGLFHERTSLSLDDSCLYEERSRDSESKVSHPVFYFSAFFIFLASNNDQLSVKDSGKGDSDFNDSDSDVSGDGGKKNFSTFQPRLKSNSSLSRDCQSAYCVIPQRSYRNSRENAYAIGFSPMPVFSNPHSIRKDCGYNTNQPKPRSNMQTFSRSGTLPSYFPQQLHETDAGQATVHQETPNIVTVATESEVATIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPT0</accession>
    <name>A0A3B5MPT0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MPT0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025793"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026103.1">
        <property type="protein sequence ID" value="ENSXCOP00000025793.1"/>
        <property type="gene ID" value="ENSXCOG00000019282.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160453"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019885">
        <property type="term" value="P:antigen processing and presentation of endogenous peptide antigen via MHC class I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05771">
        <property type="entry name" value="IgC1_Tapasin_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050380">
        <property type="entry name" value="Immune_Resp_Modulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008056">
        <property type="entry name" value="Tapasin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23411">
        <property type="entry name" value="TAPASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01669">
        <property type="entry name" value="TAPASIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="194"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="298"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="427" mass="46741" checksum="026E950DF0E84A78" modified="2018-12-05" version="1">MIELCFYVCLPADGGADVVLSCSLVEEGVGMGGMGGGSLFSRTPATLVLRDVPVGPDESLDTLTPFVPPSVPDPDLILFENKVSSPEIPNAGMLLHADCNEQEVMCEISRYTPRGLQESSDPAYFMVSLSVEGLDFSTSLILQTLPVEKDQTTLTQSKLSLPLSQSGTLLTKVIFLVFSDINSVSAPLRGDVLLNCGFKQEETPLAPDVGLEWRLQHRGRGRKVLEMKTKLDDTEEATVVHGERNGSSVDAALLLSEGNVSMSLTQLKVVDEGTYICTVNLGHFHAQQVIQLHIIQPPQVSLSEEKLVLNTNSPQTLSCHCAKYYPLDAQMEWFFLPPTETEPKLFPNQGSLSSHRQHGDGTFTLSSHVTILPSFSPGTKITCRVTHPALDDPLHISIVVEPAQPDSYWWVLGFLVVTVLFFYQVMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPU8</accession>
    <name>A0A3B5MPU8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="22">1.2.1.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = (2R)-3-phospho-glyceroyl phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>S-nitroso-L-cysteinyl-[GAPDH] + L-cysteinyl-[protein] = L-cysteinyl-[GAPDH] + S-nitroso-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:66684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:17089"/>
            <dbReference type="Rhea" id="RHEA-COMP:17090"/>
            <dbReference type="Rhea" id="RHEA-COMP:17091"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:149494"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:66685"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 22">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 21">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.12" evidence="22"/>
    <dbReference type="RefSeq" id="XP_027868142.1">
        <property type="nucleotide sequence ID" value="XM_028012341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MPU8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025813"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026123.1">
        <property type="protein sequence ID" value="ENSXCOP00000025813.1"/>
        <property type="gene ID" value="ENSXCOG00000019295.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114141659"/>
    <dbReference type="KEGG" id="xco:114141659"/>
    <dbReference type="CTD" id="2597"/>
    <dbReference type="GeneTree" id="ENSGT00940000153298"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00184"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035605">
        <property type="term" value="F:peptidyl-cysteine S-nitrosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18126">
        <property type="entry name" value="GAPDH_I_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05214">
        <property type="entry name" value="GAPDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000001">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000319">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836:SF111">
        <property type="entry name" value="GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12 22" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="11 19 22" id="KW-0520">NAD</keyword>
    <keyword evidence="19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10 22" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0702">S-nitrosylation</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="23">
        <location>
            <begin position="2"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="17">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="11"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="149"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="209"/>
            <end position="210"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="20">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU361160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="333" mass="35976" checksum="332C9CBA076FDF8F" modified="2018-12-05" version="1">MVKVGINGFGRIGRLVTRAGFTTKKVEIVAINDPFIDLEYMVYMFQYDSTHGRFKGEVKAEGDKLVIDGHKITVFHERDPTNIKWGEAGAEYVVESTGVFTTIEKASAHLKGGAKRVIISAPSADAPMFVMGVNHEKYEKSLQVVSNASCTTNCLAPLAKVINDNFGIIEGLMSTVHAITATQKTVDGPSGKLWRDGRGASQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTVRLEKPAKYDDIKKVVKAAAEGPMKGILGYTDHQVVSTDFNGDSHSSIFDAGAGIALNDNFVKLVSWYDNEYAYSNRVCDLMAHMASKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQL3</accession>
    <name>A0A3B5MQL3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome c oxidase subunit NDUFA4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the complex IV NDUFA4 subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQL3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023466"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023747.1">
        <property type="protein sequence ID" value="ENSXCOP00000023466.1"/>
        <property type="gene ID" value="ENSXCOG00000017515.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154268"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010530">
        <property type="entry name" value="B12D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14256:SF4">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT NDUFA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14256">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE MLRQ SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06522">
        <property type="entry name" value="B12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="96" mass="11384" checksum="B4C19200BBEE365A" modified="2018-12-05" version="1">RLFCREKADFSDVKMLLTIHKQLRNHPALIPLFFFIGGGAAMSLMYLARLGLRNPDVSWDRKNNPEPWNKLSPNYQYKFFTVNMDYSKLKKDRPDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRQ0</accession>
    <name>A0A3B5MRQ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sorting nexin-27</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the retrograde transport from endosome to plasma membrane, a trafficking pathway that promotes the recycling of internalized transmembrane proteins. Following internalization, endocytosed transmembrane proteins are delivered to early endosomes and recycled to the plasma membrane instead of being degraded in lysosomes. SNX27 specifically binds and directs sorting of a subset of transmembrane proteins containing a PDZ-binding motif at the C-terminus: following interaction with target transmembrane proteins, associates with the retromer complex, preventing entry into the lysosomal pathway, and promotes retromer-tubule based plasma membrane recycling. SNX27 also binds with the WASH complex. Interacts with membranes containing phosphatidylinositol-3-phosphate (PtdIns(3P)). May participate in establishment of natural killer cell polarity. Recruits CYTIP to early endosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRQ0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026458"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026779.1">
        <property type="protein sequence ID" value="ENSXCOP00000026458.1"/>
        <property type="gene ID" value="ENSXCOG00000019757.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183212"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:signal transduction"/>
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    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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        <property type="entry name" value="PX_dom_sf"/>
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        <property type="entry name" value="RA_dom"/>
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        <property type="entry name" value="SNX27_RA"/>
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        <property type="entry name" value="Ubiquitin-like_domsf"/>
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        <property type="entry name" value="SORTING NEXIN 17 AND 27"/>
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        <property type="entry name" value="SORTING NEXIN 27A"/>
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        <property type="entry name" value="PDZ"/>
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        <property type="entry name" value="PDZ domain-like"/>
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        <property type="entry name" value="PX domain"/>
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        <property type="entry name" value="Ubiquitin-like"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="41"/>
            <end position="134"/>
        </location>
    </feature>
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        <location>
            <begin position="157"/>
            <end position="265"/>
        </location>
    </feature>
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        <location>
            <begin position="269"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004220"/>
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            <dbReference type="ARBA" id="ARBA00004514"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    </evidence>
    <sequence length="521" mass="58917" checksum="7AFB96063BED46A1" modified="2018-12-05" version="1">MADVGDYEIRPAHPTASRYNGPVVGSSAGQNAATVTSGPRIVRIVKSESGYGFNVRGQVSEGGQLRSINGELYAPLQHVSAVLPGGAADRAGIAKGDRILEVNGVNVEGATHKQVVDLIRAAEKELILAVLSVPPQEADGLEGGEDVQPNYDYSDKQAVPISIPMYKHVEQHSERFVVYNVYMSGRQLCSKRYREFAILHQNLKREFSNFNFPKLPGKWPFSLSEQQLDARRRGLEEYLERVCSVRVIGESDIMQEFLSESDENYNGVTDVELRIALPDKTTISVRIRKNSTTDQVYQALVLKVGMDSIMASYFALFEVINHSFVRKLAPNEFPHKLYVQNYTSAVPGTCLALRKWLFSVQEEELLRDNPLALHYCFHQALDDVKKGFIKTEDKSYQLQKLAEQRKMATYLSLLRTCEGYNEVVFPHCSCDSRRKGHVITAISIHHFKLHACTEDGTLENQVIAFEWAEMQRWDTDEEGMAFCFEYARGEKKPRWVKIFTPYVRFGFTSSLVLSCICLLEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRU1</accession>
    <name>A0A3B5MRU1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein associated with LIN7 2, MAGUK p55 family member a</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRU1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026503"/>
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        <property type="protein sequence ID" value="ENSXCOP00000026503.1"/>
        <property type="gene ID" value="ENSXCOG00000019787.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158500"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043226">
        <property type="term" value="C:organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ_MPP6-MPP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3_MPP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MAGUK p55 subfamily member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MAGUK p55 subfamily member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000146">
        <property type="entry name" value="MAGUK p55 subfamily member 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050716">
        <property type="entry name" value="MAGUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23122">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE MAGUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00569">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="L27" evidence="10">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="L27" evidence="10">
        <location>
            <begin position="54"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="9">
        <location>
            <begin position="129"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="211"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="8">
        <location>
            <begin position="337"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="539" mass="60822" checksum="8587185D7830BFC1" modified="2018-12-05" version="1">MQQVLDNLKDMPSGTGAKDIDLIFLKGIMESPIVRSLAKAHERLEEVKLEAVGDDNVQLLTEILDSLNNLEERDPAAAELARILQEPHFKSLIEAHDKVAAKSYEMANAMDNSNSLSPSSLMPADAVRMIGIQKKTGEPLGVTFRAVNGEMVITRILHGSSIDRQGMLHTGDVIREVNGQEVGSNPEELQELLRNCSGSVTLKVLPSYRDIPAPPQVYLKPYFSYNPTTDNLIPCKEAGLAFAKGDILHVVNKEDPNWWQARKVVGGATGLIPSQFLEEKRKAFVRRDWGTAGMLCGTLTAKKKKKRMMYLTSKNAEFDRYELQIYEEVARMPPFRRKTLVLIGAQGVGRRSLKNRLVVMNPLRYGATVPFTSRHPREEEKDGQNYCFVTREEMERDIKESRYLEHGEYDGNLYGTKIDSIHEVVQAGRTCILDVNPQALKVLKTAEFMPFVVFIAAPELDTLRGMHKAVIDAGLTTKILTENDLKKTVDESARIRRAYSHYFDLTIVNDNLDKAFDKLQDTVERLFVEPQWVPVSWVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSD9</accession>
    <name>A0A3B5MSD9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BRICHOS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the chondromodulin-1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSD9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022404"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022678.1">
        <property type="protein sequence ID" value="ENSXCOP00000022404.1"/>
        <property type="gene ID" value="ENSXCOG00000016731.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00480000042679"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016525">
        <property type="term" value="P:negative regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001937">
        <property type="term" value="P:negative regulation of endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007084">
        <property type="entry name" value="BRICHOS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043405">
        <property type="entry name" value="Chondromodulin/Tenomodulin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14064">
        <property type="entry name" value="CHONDROMODULIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14064:SF3">
        <property type="entry name" value="TENOMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04089">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01039">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50869">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="BRICHOS" evidence="9">
        <location>
            <begin position="95"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="288" mass="33400" checksum="EDF37A858E068F25" modified="2018-12-05" version="1">QATNFYLNHKSSAQEGDVEAEKQRKTRFHNYHWVALVLAVVFLVLAVCIFSLMYLWGPSLGKVYDHEYKAVVDGVETNSVMEIDPADLTEIFRMGNGSKEVVEVHDFKNGITGIRFAEHQRCYIRTQTKKLPTVAEVDEAEAVQMEDSQVWVPAEEPVVNLDFLLDSKIWEICQQMPIHWIHPSPMTDAEAQDVDTPDAEVTGQRFTRDVLDHVPVNDYRDVGIELDSRLDDRGYCCQYCRRGYRFCRRYFEPLGGFNPWPYYYQGGRVICQVVMPCDWWIARMLGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MT57</accession>
    <name>A0A3B5MT57_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Inhibitor of Bruton agammaglobulinemia tyrosine kinase</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MT57"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022679"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022955.1">
        <property type="protein sequence ID" value="ENSXCOP00000022679.1"/>
        <property type="gene ID" value="ENSXCOG00000016943.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156277"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030292">
        <property type="term" value="F:protein tyrosine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18500">
        <property type="entry name" value="BACK_IBtk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18301">
        <property type="entry name" value="BTB1_POZ_IBtk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18302">
        <property type="entry name" value="BTB2_POZ_IBtk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000105">
        <property type="entry name" value="inhibitor of Bruton tyrosine kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051625">
        <property type="entry name" value="Signaling_Regulatory_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872">
        <property type="entry name" value="BTK-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872:SF2">
        <property type="entry name" value="INHIBITOR OF BRUTON TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="314"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="4">
        <location>
            <begin position="529"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="440"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="449"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1088" mass="119297" checksum="0E0D18E34DAC2A09" modified="2018-12-05" version="1">GHLTFQNNPPKTWVMAFLIYLFFAFVSRHTCAVFSKMLKGRAVIGVAAGRFHTVLWTREAVYTVGLNGGQLGYLLDPNGEKCVTTPRQVSALHHKDVGIAMAAASDGATVVVTGKGDVYLLADYQCKKMASKQLNIKKVLISGGSLDHWVHPQILNDSGGEKIAILALDEAGRVFCWRSSGSAVRQCRWAYPRQVFMSDIALSNNGMMFVTPDGEGFSGVWAGEYKKYEEKKEVSVEVSGPSDAGTMYERIRLEKLPFVHRAVSITMDSKGRNFGVLQADPRTSLFEVPSISPSSFAQHFRSLLEEADETDSIHDVTLQAGERNFPAHKYILSMRSEFFRKLFMSERCGVNEELDKEVRRGEDAVGCDLFILEKIAADMLEYALHFIYTDSCELLVPGARPRGSVMSTGQNQVSRELISSLQDLGLRGCSALEVYQSLSPSTKGDGNKAKNKGSKLSKKGKGGKGDKTRASEGGANPVKTLQAVAKHLGLGSLSARLDGVKYENGKINVVQKKTGSKPKFFQKKCSYLCDVTLKSDDGKEFPCHKSVLCARLEYFNSMLGNPWIEATSCSALEMPTSSEILQVILEYIYTDESPTIKDSPNVEFVCNVLVVADQLLITRLKEMCEVIITENLTLKNVAVLLEFATMYNAEQLKLSCLQFIVLNIGALLESKALDILSEEVLIELSAAYRRAIPAMQRRVITPYPGAPDLSAYEEEDLDLAFSPRPEGELDQTCRYTNFICAIKRKKQVVLVSCLSSSFCPAVSPVLVKTGKANSTESLQEVLTSDSEGSYMAVGSPRDMQSPVFHDKPEVILHLSFSQRLKTPPSTPTSLRFSLPVLPPTPPTSIPKDLPNPAHAPPVLDLRTIMDMEASSLQTLGDGLRTDWIRAGTVKHSTVSSKLSQKQRKMLAMASREAIVDSPPSKSVPIATPSKSSEKAWATAVQSPPPSCSFRALVEEEENRSARVGHPGPQRVQNALGTSASATPAARRVTFKNNKSGEPEKPTGPWVLGAVGSPPLSSLVTFASIVEEEKQQEAALIRSREKPLALIQIEERAIQDLLLHYKARDNPDELIAVETTSRGPMAAPTWHKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MT85</accession>
    <name>A0A3B5MT85_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Structural component of lens fiber gap junctions. Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane. Small molecules and ions diffuse from one cell to a neighboring cell via the central pore.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 11">Cell membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MT85"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022704"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022980.1">
        <property type="protein sequence ID" value="ENSXCOP00000022704.1"/>
        <property type="gene ID" value="ENSXCOG00000016962.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286930"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000002">
        <property type="entry name" value="Gap junction protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034634">
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    </dbReference>
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        <property type="entry name" value="GAP JUNCTION ALPHA-3 PROTEIN"/>
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    </dbReference>
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    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
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        <property type="entry name" value="CNX"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Connexin43"/>
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    </dbReference>
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        <property type="entry name" value="CONNEXINS_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0303">Gap junction</keyword>
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    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="14">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="15">
        <location>
            <begin position="173"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="110"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="110"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00045560"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000630"/>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="SAM" id="Phobius"/>
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        <source>
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            <dbReference type="SMART" id="SM01089"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022704.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="489" mass="54768" checksum="62B2F4B5EF12F95B" modified="2018-12-05" version="1">MGDWSFLGRLLENAQEHSTVIGKVWLTVLFIFRILVLGAAAEEVWGDEQSDFTCNTQQPGCENVCYDEAFPISHIRFWVLQIIFVSTPTLIYLGHVLHIVRMEEKRREREEDLRKSGRHQEDHDPFYNNGVSDGGGGGGRKEKPPIRDEHGKIRIRGVLLRTYIFNIIFKTLFEVGFILGQYFLYGFQLRPLYKCGRWPCPNTVDCFISRPTEKTIFIIFMLVVACVSLVLNLLEIYHLGWKKVKHGVTKEFAPDSLSLIGINEPGDAETIPEQISPTGLQYLPVSSNVCVVRGEATRGGTYSPTGTSHTVISLNTNMATVPMSEGLNVGSAACHQEGFLMESLPASIYGESEDSRGHLEEMQQNRRNMALELHNLNEENPPTYPLPSPFPSTSSTSQEEMTCLEEKQRSTFPTLPRNTPLSLLAPEETAVDPENTVATSPSIEPHDDFTVITRAEMHQPPFAVGTDLRRPSMVNKTCGARARPDDLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MTD0</accession>
    <name>A0A3B5MTD0_9TELE</name>
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        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Atherinomorphae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00023130"/>
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    <evidence type="ECO:0000256" key="3">
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            <dbReference type="ARBA" id="ARBA00043265"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="97" mass="10684" checksum="EF82C59D3EED3B57" modified="2018-12-05" version="1">EQLQQPDSLTVQPGQSLTVSCQVSYSVSSYYTAWIRQPAGKGLEWIGSKYTGDSYYKKSLENKFSIAVDASSNTVTLNGQNMQPGDSAVYYCARDPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUQ8</accession>
    <name>A0A3B5MUQ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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        </lineage>
    </organism>
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        <scope>IDENTIFICATION</scope>
    </reference>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="RRM1_RBM28_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RNA-binding motif protein 28"/>
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    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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        <property type="entry name" value="RRM_MRD1_RNA_proc_ribogen"/>
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        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN 14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48039:SF5">
        <property type="entry name" value="RNA-BINDING PROTEIN 28"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="4"/>
            <end position="78"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="89" mass="10035" checksum="DB6AFC1C301A0DE3" modified="2018-12-05" version="1">MPALTIFVASLPESATNERLEEIFSEIGPVKQCFVVREKGKCRGFGFVTYSMEEDAKRALKEIKEYDGKKLSLSVAKKKVNNKKKTGVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVZ1</accession>
    <name>A0A3B5MVZ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative treble-clef zinc-finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
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            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVZ1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023749"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024033.1">
        <property type="protein sequence ID" value="ENSXCOP00000023749.1"/>
        <property type="gene ID" value="ENSXCOG00000017736.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011031"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026049">
        <property type="entry name" value="C2orf42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029269">
        <property type="entry name" value="Zf-tcix"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13518:SF1">
        <property type="entry name" value="C2ORF42 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13518">
        <property type="entry name" value="PUTATIVE TREBLE-CLEF ZINC-FINGER C2ORF42 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14952">
        <property type="entry name" value="zf-tcix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative treble-clef zinc-finger" evidence="2">
        <location>
            <begin position="43"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="289"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="532"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="537"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="570" mass="62316" checksum="C82D8A1A4415301F" modified="2018-12-05" version="1">MDSEVIVTPSSASPQTPATRSSLAAKQRESRRSTATTPAFLSNLGRATLRGIRKCPQCGVYNGTRGLSCKNKACGESFGKEQEAGGGGAQVFSVCHRGRGTTQWGFVELVPTDTAIATGDGATLLTRINLGRCFLPSCRQGESLCVHIKQAIDCRSRATPLTFKSSVLEALQASVQAREELWRLATESPGPLVQRVSKDTLVVKCHTDSQHPLGLLHLTVAQVETQKNNRSAFHCSCQLSAGRMKHGADGAGSPPAASQPCLHFYACVCAFSSNEKLAAEFAAFQNAAGDKPAPQAQPNNSPHKAKKQRLDEPASGSRSEPDETFPVNCVGFSSGSAQVVDELTVTMGFQQWLASVTERIHQTMHYQFDGKPQPLVYHIPQEFFNALQQRLSLGSKKRRLPNLTTAFVRNDGLPLGSFSKYTWHFTNLLHVKHIFDTPELPLEVTQSFVRNLDGSYSRFHLPELPPEPDSLHGYRTERPPTIRPMEHRTFLKVGTSGDQGSSPFVIEWIPDVLPRCQMGELRVSFEFGHQQSGQSECQEKAGGAEKKARRKSDGSQPKVPKSVDVLQVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWW7</accession>
    <name>A0A3B5MWW7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Beta-2-glycoprotein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Apolipoprotein H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Beta-2-glycoprotein I</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWW7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026305.1">
        <property type="protein sequence ID" value="ENSXCOP00000025992.1"/>
        <property type="gene ID" value="ENSXCOG00000019424.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175157"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050350">
        <property type="entry name" value="Compl-Cell_Adhes-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015104">
        <property type="entry name" value="Sushi_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325:SF502">
        <property type="entry name" value="BETA-2-GLYCOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325">
        <property type="entry name" value="COMPLEMENT COMPONENT-RELATED SUSHI DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09014">
        <property type="entry name" value="Sushi_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="Sushi" evidence="14">
        <location>
            <begin position="60"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="14">
        <location>
            <begin position="183"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="185"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="214"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="335" mass="36694" checksum="54C4A2F05ED03059" modified="2018-12-05" version="1">CGSTDHLSAFYSGPCVERLQGGERKTRACPRTCKSDAECGSKRQCLCDGECGLSCVAPGRTCPWPLHSNENLVFRLVSATPSFSALLEVHCKPGLTLPDGLDVTVRRCQGDRQWSGDEPICAGGFTSPGHTPPPTCPLPEEVINTFSIHGDASVGTSIRYSCLSGSGGINTLTTIKPLCLVELFCQPPKEVQQGYVVAVQKTEYEVAFDIHYLCKKNYRLDGPQKITCLSNGSWSASPPHCRARCVIPAERSRVVIGGVKRWPFDVTDAMVPHGENVTFYCKHPKKQCSFTADQACFDGKLQTPECYLEPTWLQYKLFPHRLVSEIETCAPGDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXW7</accession>
    <name>A0A3B5MXW7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Solute carrier family 35 member F2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Putative solute transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC35F solute transporter family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXW7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024945"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025249.1">
        <property type="protein sequence ID" value="ENSXCOP00000024945.1"/>
        <property type="gene ID" value="ENSXCOG00000018625.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015655"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009262">
        <property type="entry name" value="SLC35_F1/F2/F6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052221">
        <property type="entry name" value="SLC35F_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14233">
        <property type="entry name" value="DUF914-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14233:SF12">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06027">
        <property type="entry name" value="SLC35F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="32499" checksum="6023D6363F1E2900" modified="2025-10-08" version="2">MYFSRELFKNTEKRRSAPPGSCVEQLEQIPQKYRLLKTIAMGQVLSLLICGTAGSCQYLADAGVETPMLQSFLNYALLLLTYTPTLCIHRGDANILKILRTKWWKYLLMGLADVEANYMVVKAYQFTTLTSIQLLDCFVIPVLMVLSWIFLKTRYRPLHFVAVVVCLLGVGAMVGADIVAGRNQGSGKDAFSLVFHFSSWVFTAEMIEVTWKCFCLFQSFSSERFFTTKSSIMSSVVLLDVLQVSVPVWKNLEFGPEEARLTSLFSTSIPSFVVSCFRSFLSPSLLSTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MYJ0</accession>
    <name>A0A3B5MYJ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MYJ0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027365.1">
        <property type="protein sequence ID" value="ENSXCOP00000027032.1"/>
        <property type="gene ID" value="ENSXCOG00000020193.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162818"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000087">
        <property type="entry name" value="Snaclec rhodocetin subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002353">
        <property type="entry name" value="AntifreezeII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00356">
        <property type="entry name" value="ANTIFREEZEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017228244" description="C-type lectin domain-containing protein" evidence="4">
        <location>
            <begin position="16"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="5">
        <location>
            <begin position="39"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="164" mass="18556" checksum="99A28C2526D043D0" modified="2018-12-05" version="1">TPMILLFFLFGLSLAAVAPSDKQEMKLQRGGCPLFWFIFNGRCYKYVATLMTWADAEQHCVTQGANLVSIHSLEEENFVKLLIKNFDPAQGVNWIGLSDAQKDGTYFWSDGSTFNFNFWNTGEPNNAGGSEPCVHTNWGPARRWNDKVCTDAHSFVCKLRPYCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MYT1</accession>
    <name>A0A3B5MYT1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TIL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MYT1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027187"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027520.1">
        <property type="protein sequence ID" value="ENSXCOP00000027187.1"/>
        <property type="gene ID" value="ENSXCOG00000020302.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160698"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036084">
        <property type="entry name" value="Ser_inhib-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002919">
        <property type="entry name" value="TIL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01826">
        <property type="entry name" value="TIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57567">
        <property type="entry name" value="Serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017387857" description="TIL domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="TIL" evidence="2">
        <location>
            <begin position="24"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="88" mass="9858" checksum="5FEA895309AE4155" modified="2018-12-05" version="1">CSFTTPLSTVATILPHLSAVSLRCPPGKEYQPCVSTCNSRTCLNKDYYEETTCSFIREECVCRSGTILHRADSPYCVTEDRCGEWRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N082</accession>
    <name>A0A3B5N082_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Membrane bound O-acyltransferase domain containing 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027896488.1">
        <property type="nucleotide sequence ID" value="XM_028040687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5N082"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026410"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026730.1">
        <property type="protein sequence ID" value="ENSXCOP00000026410.1"/>
        <property type="gene ID" value="ENSXCOG00000019715.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114158811"/>
    <dbReference type="KEGG" id="xco:114158811"/>
    <dbReference type="CTD" id="692278"/>
    <dbReference type="GeneTree" id="ENSGT01030000234564"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF7">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="382"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="420"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="452"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="482"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="493"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="505" mass="57470" checksum="6B7756F9DE607BD8" modified="2018-12-05" version="1">MADESTRTTACTGSSLLQPVSEMTNLPLDQVNFVVCQLCALISAFWFRLFLHPSKTSPFIRHVVATVLGLYFALFCFGWYALHFLLQSGLTYGIMILTGVEHMHKYCLVVALSYLSLCQITRVYVFDYGMYSADFTGPMMVITQKITSLAFEIHDGMARKEEHLTAGQKILAIRRMPSLLEYFSYNCNFMGILAGPTCSYNDYIAFIEGKPHRHREQEADKSKTRLGQGEPSPNMEVVRKVATSFFCLLVFLSVCKVFPVERNIEDDFIANTPFYAQVVYLYLSMLTTRPKYYFVWTLADAINNAAGFGFNGYDSNGAPRWDLISNLRILNIELATSFKVFLDNWNIQTVQWLKRVCYERCPYHPTAATFILSAMWHGAYPGYYLTFLTGIVVTLAARAVRHKVRPHFLHSAAHKLFYDVITWAATQIAICYTVVPFVLLSVGPSLKFYRSWYFSLHIMCILLAVALPVKPRHLRLKEQQKSCSQDAGLKPLEAADTHSHKEKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N089</accession>
    <name>A0A3B5N089_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DnaJ homolog subfamily C member 8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Suppresses polyglutamine (polyQ) aggregation of ATXN3 in neuronal cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with SRPK1. Interacts with HSP70 (HSPA1A or HSPA1B).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027891227.1">
        <property type="nucleotide sequence ID" value="XM_028035426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5N089"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026425"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026745.1">
        <property type="protein sequence ID" value="ENSXCOP00000026425.1"/>
        <property type="gene ID" value="ENSXCOG00000019679.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114155513"/>
    <dbReference type="KEGG" id="xco:114155513"/>
    <dbReference type="CTD" id="22826"/>
    <dbReference type="GeneTree" id="ENSGT00390000012569"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000026">
        <property type="entry name" value="dnaJ homolog subfamily C member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042858">
        <property type="entry name" value="DNAJC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15606:SF4">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15606">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 8/LIPOPOLYSACCHARIDE SPECIFIC RESPONSE-7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="10">
        <location>
            <begin position="55"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="175"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="175"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="250" mass="29583" checksum="9000EC473ADBCD51" modified="2018-12-05" version="1">MAAAGGESSSQSVSDELFQNFYTEVKQIEKRDSVLTSKQQIDRLLRPGSSYFNLNPFEVLQIDPEATDEELKKRFRALSILVHPDKNQDDPDRSQKAFEVVDKSYKLLLDPEQKKRAVDVIQAGKEYVEHMVKQKKKQLKKDGKPQDVEEDDPDMFRQAVYKQTMKLFAELEIKRKEREAKDMHERKRAREEEIEAAEKAKREREWQKNFEESRDGRVDSWRTFQTKGKSKEKKNRSFLKPPKVKMEQRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N0G4</accession>
    <name>A0A3B5N0G4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Junctional cadherin 5 associated a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000026605"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026929.1">
        <property type="protein sequence ID" value="ENSXCOP00000026605.1"/>
        <property type="gene ID" value="ENSXCOG00000019864.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166891"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903589">
        <property type="term" value="P:positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028221">
        <property type="entry name" value="JCAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34757:SF1">
        <property type="entry name" value="JUNCTIONAL CADHERIN 5-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34757">
        <property type="entry name" value="JUNCTIONAL PROTEIN ASSOCIATED WITH CORONARY ARTERY DISEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15351">
        <property type="entry name" value="JCAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="416"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="470"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="588"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="748"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="948"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1303"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="484"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="550"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="588"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="610"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="639"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="691"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="748"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="777"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1047"/>
            <end position="1064"/>
        </location>
    </feature>
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        <location>
            <begin position="1066"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1164"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1313"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1350"/>
            <end position="1366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1366" mass="151409" checksum="B31BD181399585CA" modified="2018-12-05" version="1">MYSVEDLLISHGYKLPKSGPPSAAPYDKRPADCQRELVDSRAGRGTLNGYEAERGASITGIYDSRQALVKGYPATDESGERILRHRQHWFIQDSVLLTLPLYFVCIYRFYDVPSLTYSEPLSHDERDISYWRRRGQDFSILLDYADGRELRASAGAWRPQALITAEEYRAERQAQQLWEEISWKCQSLGTEEWKPAVGMGRQLSDGDGERWAKDQYRLRTPEGFFHPRTKAKSQSLPRVLSPDRTDCRDLILSRPSLPDSRYVYGGGRNGWGRNVGPSSHVALLPKPRFSRPLKPPSYEIHQQTRGSAEMLAIDQGAKQKDRSIYYPRSGDLRQDYYAQHSAITGMEPPGYIPPPSYKRAPPPRAVSVNRNEMINLRWRAEALQMPSSDPGRWLSRQGSSSWVGHYGERSASYRKLVQPGPDEQPVQARQLSIEDPRVKQISGGPGGHSLTDLDKLRNINKEIQFAKTLGQSTHDSAFPPQQGPAPNTEANKTAPSDNESSNRWCNRGIKKSESVGPEQSRPIIFPSSFLGKPPPPPCKPADQGASETVTEAKKVEQHEPPEKEKTKNLKKRLSETIFCLVSVPVTPQLTGTIRDQNNNNEKSPDPAGSPSDNKTGHLTNQSLQSTSSAEAELQALTGSFASSKTSSRASSKMFKKLPCRPPKINPYKELKLSGTWPANQYRDQETQTSPEARKPAPENKEAQQEPVPSGTDVAPDSSAVVGTAFSFPIKGVKSLKLSSNSAFSLTSTFSSQLNKSTAQQPAVPPSGNVEEAKPAASSEQFLIKPTTQRPWDAVKELESMKKEIQDQQQQQISKQQSVDKCIEDLNEAYKDILELGTASNKVPNGSVQIPERIKIRLTSEPLNKPSSLRRSAVSWSVDPEYREVKSAFSRPATKSVTFSKQLREELPVPPRETGFREYRVVAHLSRRRSNDGRTVKLDLPDEPIETPLCDFSPMTHNTAAEVPWADRQPMQDASTLTSPPDYEDICQTLRQSKDSADGNRVSTGQSRPNDTEPLSGESEEECPICKRELENQMRQGPLPPLHEENSSDSSANQNGSPPQCSTLESPGKDSNIEEPKDLSSNQPGTDLNPEALEQQSVTQDANAGEKGGDAQAENSGTVDPAEGKPENEANDDPSSASAVLQTDPPADLQVTEEQHANEIVATKVELKEEKEKAISGKTPEVKKKMVVKGERQNNTLPREKKAQEKKSVSVPELRIGLRSHLGRDHPGLPEFPPDRLPLSVPPNLDRRLSLSLEGERRTKGPSQRIEALQNKLAISPGHVPVERLARMREVDVICRVRRLSIRSTDSGEGEAEGEGKEDHGEELHRVPQEGKENNQEVTHSEQGAEESVLQDGQKSSLSGENKNSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N0Q6</accession>
    <name>A0A3B5N0Q6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">VWFD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5N0Q6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026820"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027149.1">
        <property type="protein sequence ID" value="ENSXCOP00000026820.1"/>
        <property type="gene ID" value="ENSXCOG00000020026.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156076"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050780">
        <property type="entry name" value="Mucin_vWF_Thrombospondin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014853">
        <property type="entry name" value="VWF/SSPO/ZAN-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339">
        <property type="entry name" value="EXTRACELLULAR MATRIX GLYCOPROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339:SF384">
        <property type="entry name" value="MUCIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08742">
        <property type="entry name" value="C8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00832">
        <property type="entry name" value="C8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="VWFD" evidence="5">
        <location>
            <begin position="43"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="4">
        <location>
            <begin position="378"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="54848" checksum="A15EA27789207CCF" modified="2018-12-05" version="1">EKGKEVTEHVPCKSAPIPVCDNGFEPIKVFDKGGCCFQYECKCLCTGWGDPHYNTFDGQYYSFQHNCTYVLVKEIIPKYNLKILIDNENCDPSGTVTCPKALIIYYKNYEVTLTQNRTPKTQNLIYVNGTKVTPPYSNKDFIITSAGIQMVLEIPEIDATVSFTGLLFSIDLPFSLFHSNTEGQCGYCDNNKKNDCRLPNGEIHPLCSEMAYNWHVKDKNKSYCESVPTRPPPIIEPTKPPCKPALCEIINHEMFKNCHKKIAPNSYYEACKFDVCQMPNSTVGCTSLAAYAMMCAGESECIDWRNLTKGECGYKCPENKVYKACGPSIVETCNAGFNNKLKQQCAGQEATCNGLVEGCFCPVGTMLFGPGSDTCVSSCCTGPAGEPKQIGDTWKSGCQQCNCDKSSLSVKCEPLVCPTQKPIKCTEDGEVLVNQTVNCCQELTCGKQFQLPGANFSKNPCEHCLCTNKQDPKTKLNTIECQQIQCNVICKKVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KPX8</accession>
    <name>A0A3B5KPX8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histone H3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KPX8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000522"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000531.1">
        <property type="protein sequence ID" value="ENSXCOP00000000522.1"/>
        <property type="gene ID" value="ENSXCOG00000000456.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286903"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000047">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="4">
        <location>
            <begin position="44"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="120" mass="13963" checksum="E277AD732B83C3C5" modified="2018-12-05" version="1">MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQNGGLVVPWILSWRTLRWRSAPPLPYNMLQKQFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRP5</accession>
    <name>A0A3B5KRP5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ethanolamine kinase</fullName>
            <ecNumber evidence="5">2.7.1.82</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the choline/ethanolamine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.82" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRP5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000316"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000323.1">
        <property type="protein sequence ID" value="ENSXCOP00000000316.1"/>
        <property type="gene ID" value="ENSXCOG00000000283.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182939"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004305">
        <property type="term" value="F:ethanolamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603">
        <property type="entry name" value="CHOLINE/ETHANOALAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603:SF68">
        <property type="entry name" value="ETHANOLAMINE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01633">
        <property type="entry name" value="Choline_kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="2" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="349" mass="40493" checksum="1CA087EF850966C0" modified="2018-12-05" version="1">SSGVTKCSGDQLRHLDVAVDEREPRRGILDLLSKLRPEWKTQHIQMKKFTEGITNQLIGCYVGSLQEPGCVLVRLYGRMTELYVNRDREVEMFQVFNAHGCGPEIYCSFQNGICYEFVRGNVLEDELLRQPSIYRLIAAEMGKIHSIQPKRGQTTEPQLWAKMSHFLTLVKSSITRNPSLILSNHFFDKNQSQLLSLRSTLFLHHILAYSDLLTGFQSTVVGGKFFPLFSVLRFFILLSQNWSNSVTDVDYSLYPSQELQRDWLTAYLESYKRSTGLNVTVTDEEVTKLYVEVCKFSLASNFFWGLWAILQSRFSSIDFDFQRYAIARLSYYFEKKEEYFELTLSQLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRT6</accession>
    <name>A0A3B5KRT6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRT6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000615.1">
        <property type="protein sequence ID" value="ENSXCOP00000000605.1"/>
        <property type="gene ID" value="ENSXCOG00000000521.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160991"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF14">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="9">
        <location>
            <begin position="41"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="279"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="132"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="197"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="389" mass="41932" checksum="B4B9A27F9B24EFF0" modified="2018-12-05" version="1">MDPTCCDSVAPPSLTVQVCFPGVRAAVLDNLNRQREEGRLCDLSIQVQGQVFRAHRCVLAASSPYFHDQVLLKNVTTVSLPSVLDPVAFQSVLSSAYTGQLSIIHDDIVNYVTVASFLQMWHIVDKSAPSACTPSEKAAAGTGSRMRCRRWRHGGGRSRRRGGPGRGLPPSRTRPTPNDGDIDLECFGSFDEIEDGTGHVSQRPLLSPDFNMTDGNWPSSSAAFPPQPPSPPTPSSSSSASGAGGYAGKVHLCHCGKAYTLKSMRDRHVKMQHLNLRPFGCPVCTKSFKMKHHLTKHLKTHGGLRPYECSLCGKKVVWRDSFLRHQARCQRLASVGGGVATATAAELEDRYGLGFDDGEVVGLTPADPPLLPGVVLVPSDLSGGGRRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KSC3</accession>
    <name>A0A3B5KSC3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Leucine rich repeat containing 43</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KSC3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001377"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001400.1">
        <property type="protein sequence ID" value="ENSXCOP00000001377.1"/>
        <property type="gene ID" value="ENSXCOG00000001122.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008994"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454:SF19">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="452" mass="50815" checksum="946927D0E5228D85" modified="2018-12-05" version="1">MSSKTLSAAIEKNIRRLGLTDFPCGNGPWVSFHCPRRAKNGPDEAETESPDVLLDLLRYPRSPWMYSETWSPQASELRELAVKEPKRLSKNFILNYFTTIRITGKNVSSIDKGLLKFSKLEELVLSGNLISEIPAKHLPSTLKILELRANRLSSLSDLASCPPPQLQYLGLGSNSLGSHQDICDLTGRHWPHLACLDLSNCNFQEQQFLVATLETLPCLKTLTLKGNPFTMTPSYPGFTVDSLPQISCLDSSWLTTEERYNFFGLARMNDLVEDVASVTLSLTRLRGIPDPLMHVDKKAVEFPIVTYKYFISYQFFSHPTPGYQCSVLLLVSIINLFDKNSCFSNKYKFIYEMLFLTKENVQVDGVKIENMTSSFCSVLKVKTPPKPKNKKKKGPPELVHDPPIRKLLNSVHVPMQSLVDGGQRVNTVCDFGPLQVEPPPKQEEASFSCIVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTW5</accession>
    <name>A0A3B5KTW5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1</fullName>
            <ecNumber evidence="5">3.1.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">HD domain-containing protein 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Metazoan SpoT homolog 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Penta-phosphate guanosine-3'-pyrophosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">ppGpp hydrolyzing enzyme involved in starvation response.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>guanosine 3',5'-bis(diphosphate) + H2O = GDP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14253"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:77828"/>
            <dbReference type="EC" id="3.1.7.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MESH1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.7.2" evidence="5"/>
    <dbReference type="RefSeq" id="XP_027870077.1">
        <property type="nucleotide sequence ID" value="XM_028014276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTW5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003558"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003597.1">
        <property type="protein sequence ID" value="ENSXCOP00000003558.1"/>
        <property type="gene ID" value="ENSXCOG00000002804.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114142805"/>
    <dbReference type="KEGG" id="xco:114142805"/>
    <dbReference type="CTD" id="374659"/>
    <dbReference type="GeneTree" id="ENSGT00390000011608"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008893">
        <property type="term" value="F:guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000012">
        <property type="entry name" value="HD domain containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052194">
        <property type="entry name" value="MESH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46246">
        <property type="entry name" value="GUANOSINE-3',5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE MESH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46246:SF1">
        <property type="entry name" value="GUANOSINE-3',5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE MESH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13328">
        <property type="entry name" value="HD_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="13">
        <location>
            <begin position="32"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="179" mass="20340" checksum="105FE81F750D82C3" modified="2018-12-05" version="1">MNSEAALLLETVNFAAEKHRKQRRKDPEETPYINHPIGVARILSYEGGVTEIEVLQAALLHDTVEDTDTTPEELVDRFGPIVARIVQEVTDDKSLPKHERKRLQVEHAPHCSRQAKLVKMADKLYNLRDLNACTPVGWSAERVQEYFVWAAEVVKGLKGTNSALEEKLEDQFKQRGVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTY0</accession>
    <name>A0A3B5KTY0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Voltage-dependent calcium channel gamma-4 subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Regulates the activity of L-type calcium channels that contain CACNA1C as pore-forming subunit (By similarity). Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs), including GRIA1 and GRIA4. Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization and by mediating their resensitization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027898941.1">
        <property type="nucleotide sequence ID" value="XM_028043140.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027898942.1">
        <property type="nucleotide sequence ID" value="XM_028043141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTY0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001071"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001084.1">
        <property type="protein sequence ID" value="ENSXCOP00000001071.1"/>
        <property type="gene ID" value="ENSXCOG00000000877.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114160515"/>
    <dbReference type="KEGG" id="xco:114160515"/>
    <dbReference type="CTD" id="794222"/>
    <dbReference type="GeneTree" id="ENSGT01050000244893"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032281">
        <property type="term" value="C:AMPA glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016247">
        <property type="term" value="F:channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099590">
        <property type="term" value="P:neurotransmitter receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098943">
        <property type="term" value="P:neurotransmitter receptor transport, postsynaptic endosome to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051968">
        <property type="term" value="P:positive regulation of synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098970">
        <property type="term" value="P:postsynaptic neurotransmitter receptor diffusion trapping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019226">
        <property type="term" value="P:transmission of nerve impulse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000004">
        <property type="entry name" value="Voltage-dependent calcium channel gamma-4 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051072">
        <property type="entry name" value="CACNG_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005423">
        <property type="entry name" value="VDCC_g4su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008368">
        <property type="entry name" value="VDCC_gsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12107">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12107:SF7">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-4 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01792">
        <property type="entry name" value="VDCCGAMMA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01603">
        <property type="entry name" value="VDCCGAMMA4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 15" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="6 15" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <keyword evidence="10 15" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="178"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="237"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="320" mass="35570" checksum="CDEA1CBE29534DE3" modified="2018-12-05" version="1">MGWCDRGVQTLLATAGAFAAFSLMTIAIGTDYWLYSRAYICNGTNVTTDEIKTKKGDLTHSGLWRICCIEGINQGSCYWINHLSDDDDYDTDRSEYLLRVVRAFSVFPILSTTLLMLGGLCVGVGRAYNKTNNVLLSAGILFVAAGLSNIIGIIVYISSNTGDPGDKRDDDKKYTYSYGWSFYFDALSFIVAETVAVLVINIYIEKNKEIRWKARREFIRSLSSSSPYSRIPSFRYRRRNSRASSHSTEASRENSPAGGMKGATSSSGPLDELSMYALARDSVTENTYSPEHEGSAEFLQVHNCFPNDLKDGVNRRTTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KU85</accession>
    <name>A0A3B5KU85_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Synaptosomal-associated protein 29</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Soluble 29 kDa NSF attachment protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Vesicle-membrane fusion protein SNAP-29</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. SNAP29 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Also plays a role in ciliogenesis by regulating membrane fusions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Forms a SNARE complex, composed of VAMP8, SNAP29 and STX17, involved in fusion of autophagosome with lysosome. Interacts with multiple syntaxins including STX6. Interacts with EIPR1. Interacts with STX17; this interaction is increased in the absence of TMEM39A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cell projection</location>
            <location evidence="16">Cilium membrane</location>
            <topology evidence="16">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Cytoplasmic vesicle</location>
            <location evidence="17">Autophagosome membrane</location>
            <topology evidence="17">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNAP-25 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027890543.1">
        <property type="nucleotide sequence ID" value="XM_028034742.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027890544.1">
        <property type="nucleotide sequence ID" value="XM_028034743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KU85"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002067"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002097.1">
        <property type="protein sequence ID" value="ENSXCOP00000002067.1"/>
        <property type="gene ID" value="ENSXCOG00000001663.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114155073"/>
    <dbReference type="KEGG" id="xco:114155073"/>
    <dbReference type="CTD" id="9342"/>
    <dbReference type="GeneTree" id="ENSGT00950000182843"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031629">
        <property type="term" value="P:synaptic vesicle fusion to presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016082">
        <property type="term" value="P:synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15856">
        <property type="entry name" value="SNARE_SNAP29C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15887">
        <property type="entry name" value="SNARE_SNAP29N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000041">
        <property type="entry name" value="Synaptosomal-associated protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000051">
        <property type="entry name" value="synaptosomal-associated protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305">
        <property type="entry name" value="SYNAPTOSOMAL ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305:SF9">
        <property type="entry name" value="SYNAPTOSOMAL-ASSOCIATED PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0969">Cilium</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="23">
        <location>
            <begin position="48"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="23">
        <location>
            <begin position="195"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="147"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00037808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00037854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00043032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00046522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="258" mass="29083" checksum="90F4A8027CA48C38" modified="2018-12-05" version="1">MAYPTSLNPFADDNEEDFKPKRSGFEDESDSGMTDAERQQRYLQQEVMRTAQSAVDSSYRSLSLIYESEKMGVETAEELVRQGEVLKRTDKMLDNMDEDLKTSQRHITSIKSVWGGLVNYFKGKPETKPTPEEPKAYQANERLQTAMSGSREHEDKYQASHPNLRKLDTGGFGAAASMDDSSSRQNGYPQNRYLRDAHQTLDNNLDEMSSGLARLKNLGLGLQAEINDQDDSIGSLLNKVDTMDAKIINTNQQLKNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KV32</accession>
    <name>A0A3B5KV32_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KV32"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002387"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002421.1">
        <property type="protein sequence ID" value="ENSXCOP00000002387.1"/>
        <property type="gene ID" value="ENSXCOG00000001905.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01080000257344"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001230">
        <property type="entry name" value="Immunoglobulin kappa variable 8-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="1">
        <location>
            <begin position="12"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="115" mass="12731" checksum="19F5CEFF59D50052" modified="2018-12-05" version="1">TFNLCFWYQSRGQVTVTQPSAVNTGFGGSATISCKTSQAVYYDGSDYFLAWYQKKDTEPPKMLIYWGNRRASGIPNRFTGSGSNSDFTLTISGVQAEDAGVYYCQSYHSGPVFTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXC0</accession>
    <name>A0A3B5KXC0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXC0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003234.1">
        <property type="protein sequence ID" value="ENSXCOP00000003197.1"/>
        <property type="gene ID" value="ENSXCOG00000002516.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154852"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="309" mass="34819" checksum="A8A944CDD3845C5E" modified="2018-12-05" version="1">MLPYFGAAIVFSPNSFQAVITLLLGETIRNRFFLSVLQVGLPEEMVKRCMQQLGLALDFMHSKNLVHRDVKPENVLLFDRECRRIKLADFGMTRRVGCRVKRVSGTIPYTAPEVCRANRAEGFLVTTSMDVWAFGVLVFCMLTGNFPWEAAMPADAFYEEFRRWQKAGCPAGTYPSQWRRFTDDALRMFQRLLAAEPEKRCGVKDIFCFIKYELVSELRRRASYRAKRGERSSSGVCTGSCTASSSRSSIRHPEPSTPPGTSCLRPAPLKRSVLSDPLSPREEAGQHQAPGRDKNKSQMVMATAIEICV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXK7</accession>
    <name>A0A3B5KXK7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">E3 ubiquitin-protein ligase component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling. The retrotranslocation channel is composed of PEX2, PEX10 and PEX12; each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane. PEX10 also regulates PEX5 recycling by acting as a E3 ubiquitin-protein ligase. When PEX5 recycling is compromised, PEX10 catalyzes polyubiquitination of PEX5 during its passage through the retrotranslocation channel, leading to its degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXK7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002765"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002802.1">
        <property type="protein sequence ID" value="ENSXCOP00000002765.1"/>
        <property type="gene ID" value="ENSXCOG00000002196.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048446"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16527">
        <property type="entry name" value="RING-HC_PEX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000332">
        <property type="entry name" value="Peroxisome biogenesis factor 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025654">
        <property type="entry name" value="PEX2/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23350">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23350:SF0">
        <property type="entry name" value="PEROXISOME BIOGENESIS FACTOR 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="274"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="327" mass="37099" checksum="CE12B2F49694A481" modified="2018-12-05" version="1">MPLAPANQAQLIRSSQKDEFYRSLLRNSANEACQSVAGSKHWLDWRREVELLSDLAYYGLTTLSGYQTLGEEYVGIVQVDPSRRQIPSRARRCLFVLCHAFVPYLLEKALVCLENELEGGLEARRRQAASGAWSLERWLRRAAQSAVGLLTERQRKACQPAVFVIQQSLALLHRLHVALFYVSSSFYHMSKRAAGIGYLCVTGPNADDSSIRRSYRLLGCVSFLQLLVTLSLQLNNLRQRQRARQDATTRHSLLCVISPRHPASSASAPRPARCILCLEARRNSTCTPCGHLFCWECIAEWCNTKAECPLCREKFLPHRLVFLRNYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXY6</accession>
    <name>A0A3B5KXY6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXY6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003422"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003460.1">
        <property type="protein sequence ID" value="ENSXCOP00000003422.1"/>
        <property type="gene ID" value="ENSXCOG00000002700.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168428"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000228">
        <property type="entry name" value="obscurin isoform X4"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:000707">
        <property type="entry name" value="Obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
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    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052385">
        <property type="entry name" value="Obscurin/Obscurin-like_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35971:SF5">
        <property type="entry name" value="OBSCURIN LIKE CYTOSKELETAL ADAPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35971">
        <property type="entry name" value="SI:DKEY-31G6.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="29"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="121"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="217" mass="24613" checksum="D3AF5E01D2C9AD30" modified="2018-12-05" version="1">LFSMHNQMMTKQKTQGNVNNKHFLHIVAPPVTFKTKLRNQQVEEENSVMLSCELSKPGLAVEWMKGKELLKSDFKYQIKNRNSIMELTIKNTQLEDSGLYSCMCGDVKTTAHVTITCKKLPPFFKDNLKSLTIEEGGTASLCCEVSKPGLPVQWKKNRLPLRANRKYEMKHDDCFLQLYIKDLTLDDSGSYSCQAGDVETTATVTVKGYNLLLPKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KY00</accession>
    <name>A0A3B5KY00_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SP-RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KY00"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002905"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002942.1">
        <property type="protein sequence ID" value="ENSXCOP00000002905.1"/>
        <property type="gene ID" value="ENSXCOG00000002314.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="F:SUMO ligase activity"/>
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        <property type="term" value="F:transcription coregulator activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:protein sumoylation"/>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
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    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
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        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782:SF12">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE PIAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10782">
        <property type="entry name" value="ZINC FINGER MIZ DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02891">
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    </dbReference>
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        <property type="entry name" value="ZF_SP_RING"/>
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    </dbReference>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
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        <location>
            <begin position="82"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="100"/>
            <end position="115"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="115" mass="13108" checksum="DF295DA118DD469C" modified="2018-12-05" version="1">MCPLGKMRLTVPCRAVTCSHLQCFDAALYLQMNEKKPTWICPVCDKKADYENLIIDGLFLEILNDCSDVDEIKFQEDGTWCPMRPKKDPVKRSSKYSPHRPTTSRVEVSSTPSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYD5</accession>
    <name>A0A3B5KYD5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Zebrafish testis-expressed 38</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KYD5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002646"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002681.1">
        <property type="protein sequence ID" value="ENSXCOP00000002646.1"/>
        <property type="gene ID" value="ENSXCOG00000002111.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
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        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051294">
        <property type="entry name" value="HORMA_MeioticProgression"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48225">
        <property type="entry name" value="HORMA DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48225:SF4">
        <property type="entry name" value="ZEBRAFISH TESTIS-EXPRESSED 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0469">Meiosis</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="HORMA" evidence="7">
        <location>
            <begin position="19"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="308" mass="35700" checksum="B8A91AC8F50AD744" modified="2018-12-05" version="1">VFNEQKSREFHLIILHETGCSLVFVKRMMAIAVSSVTYLRGIFPESAYRSRYLDDLCIKVLRENSNTPGANKVVKWMMGCFDALEKQYFVLFNLQCIIESYQFNFRYTEMGPEMDISRWGRISFCSKASVLLIRKLFLLLQNLDALPSNVYLTMKLYYYDDITPADYQPPGFQEGVHDRLWFQGVPVHFKVGQVHTAFHSLRVRVSVEQGQVKKLNRNGDYATSVFLIPLFCFCVYIFFSLIAHRPCKDACSQTHCCVVGSVQNLLFCFKGQHRHDRTEDFISHTRHVVCAVACVKVKDGQQLYLDNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYW4</accession>
    <name>A0A3B5KYW4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NOP14 nucleolar protein homolog (yeast)</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KYW4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003230"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003267.1">
        <property type="protein sequence ID" value="ENSXCOP00000003230.1"/>
        <property type="gene ID" value="ENSXCOG00000002527.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017459"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007276">
        <property type="entry name" value="Nop14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183">
        <property type="entry name" value="NOP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04147">
        <property type="entry name" value="Nop14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="137"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="318"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="188"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="356"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="863" mass="98378" checksum="17D7296E003D5256" modified="2018-12-05" version="1">MGKAPKKKNLADKVRKAKAASEIKNNPFEVKINRKKFDVLGRKSKHDVGLPGVSRSKAISKRKETLLKEYKQKNKSNKFIDRRLGEYDAKMTPEDKILQRFAIERQHSHGKRDVYNLNEEEELTHYGQSLAEIEKFNDTVGSDDESEEKGLLSAELTASHFGGGGGLLRKKTSGGEQEDEQGGQKAKSRQELIEELIQKSKQEKRERQVQKEEAQELTEKLDQDWKSIQALMVKKTPKAEHVDKPEEKPEEYDMMVRELGFEMKAQPSEKMKTPEELAKEEKEKLQKLEADRLRRMMGDEDGASVQSQVHMSADDLNDGFVLDKNDKKTLAYQDGKWNIEEETEDDKEEEGKTGEEEGSEAEDENEEDDDDVEEEEEEGSGEDEDGHSDLESEQESENEENDEEEEASSSRQQQTLSREELKAQQEAAKAELPYTFTAPESYEDLKDLLHGHTPDNQRLIMARTQKCNHPSLAAGNKLKLQKLVGFLMEYMGELATRSPPELCTIDKLIPELYALCQMFPEAAGKSMQSILGDAAHSMEEAVEVKGHAAFPTLDMLVYLKVTALLFPTSDFRHPVTTPALLFISQTLTKCPVRSLQDLTSGLVLCCLGVEYISFSKRFLPELINFLSGTLHLAVQDKASTGCMVVPPFRLSGKYSDLLVLSNSESCSSWSKKNLPLSATQQLELKSDLNKDHYRLNCLSTCLDLVKRCCLLYKDLTSFTHIFQPIRTLLSKHLLFVVLLQELHSEILDAISSASVTHTRLVFEKKKPIPLKLFTPKIVEVLDYGKKRGSTREEREKERLKHKYKKEFKGALREIRKDSRFLAREKLNEVMNRDAERKRKVKELLGSLATQEGEWKALKRKKRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYX5</accession>
    <name>A0A3B5KYX5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the phosphatase 2A regulatory subunit B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KYX5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002836"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002873.1">
        <property type="protein sequence ID" value="ENSXCOP00000002836.1"/>
        <property type="gene ID" value="ENSXCOG00000002254.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182864"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000009">
        <property type="entry name" value="PP2A_PR55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018067">
        <property type="entry name" value="PP2A_PR55_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11871">
        <property type="entry name" value="PROTEIN PHOSPHATASE PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037309">
        <property type="entry name" value="PP2A_PR55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00600">
        <property type="entry name" value="PP2APR55"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01024">
        <property type="entry name" value="PR55_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01025">
        <property type="entry name" value="PR55_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00064415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU331113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="425" mass="49074" checksum="186FC7C542E800A6" modified="2018-12-05" version="1">ADIISTVEFNHTGELLATGDKGGRVVIFQREPESKSEPFSQGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQQNAAHFLLSTNDKTIKLWKVSERDKRPEGYNLKDEEGRIKDISTVTSLQVPVLKPMDLMVEVSPRRVFANAHTYHINSISVNSDYETYMSADDLRINLWHLDITDRSFNIVDIKPANMEDLTEVITAAEFHPLHCNLFVYSSSKGTLRLCDMREAALCDKHSKLFEEPEDPSARSFFSEIISSVSDVKFSHSGRYLLTRDYLTAKVWDLNMESKPLETYQVHDYLRSKLCSLYENDCIFDKFECAWNGSDSVIMTGAYNNFFRMFDRNTKRDVTLEASRESSKPRAILKPRRVCATGGKRRKDDISVDSLDFTKKILHTAWHPNENIIAIAATNNLYIFQDKLNSEMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZY9</accession>
    <name>A0A3B5KZY9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZY9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004161.1">
        <property type="protein sequence ID" value="ENSXCOP00000004117.1"/>
        <property type="gene ID" value="ENSXCOG00000003234.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155735"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051493">
        <property type="term" value="P:regulation of cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051234">
        <property type="entry name" value="TAO_STE20_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47167">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TAO1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47167:SF10">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE TAO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="93" mass="11056" checksum="1C66132C534F6F72" modified="2018-12-05" version="1">MELLDAYQKKTKSQMETQHERELQKLEQKVSIRRAHLEQKIEEELAALQKERTERIKHLLERQDRELCAFDSESRSLGFGSLGSLDFPKEDNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L013</accession>
    <name>A0A3B5L013_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L013"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003216"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003253.1">
        <property type="protein sequence ID" value="ENSXCOP00000003216.1"/>
        <property type="gene ID" value="ENSXCOG00000002533.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159664"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF33">
        <property type="entry name" value="VOLTAGE-DEPENDENT T-TYPE CALCIUM CHANNEL SUBUNIT ALPHA-1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="295"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="13">
        <location>
            <begin position="75"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="312" mass="35968" checksum="0A7E19B94AB977E8" modified="2018-12-05" version="1">YRGLCLRTLVHPSFSLHSSVISKLAPFARTYAQQPFHKSKNVEVVGNIKRLISCWRVWCMSFSNIWLVDAALTHVFWSLSVSFSSFFLFSRMLEYSLNLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPMLGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFVKDNFSFPLSVELGKYYHTENDDENPFICSMPRDNGMRDCGSIPKLYDEAGQQCSLDIDSYNSTDNTTCVNWNQYYTNCSAGLINPFKGAINFDNICYAWIAIFQVITLEGWVDIMYFVMDAHSFYNFIYFILLIIVSPSCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1X2</accession>
    <name>A0A3B5L1X2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Metallo-beta-lactamase domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Endoribonuclease MBLAC1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Endoribonuclease that catalyzes the hydrolysis of histone-coding pre-mRNA 3'-end. Involved in histone pre-mRNA processing during the S-phase of the cell cycle, which is required for entering/progressing through S-phase. Cleaves histone pre-mRNA at a major and a minor cleavage site after the 5'-ACCCA-3' and the 5'-ACCCACA-3' sequence, respectively, and located downstream of the stem-loop. May require the presence of the HDE element located at the histone pre-RNA 3'-end to avoid non-specific cleavage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a ribonucleotidyl-ribonucleotide-RNA + H2O = a 3'-end ribonucleotide-RNA + a 5'-end 5'-phospho-ribonucleoside-RNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68096"/>
            <dbReference type="Rhea" id="RHEA-COMP:15179"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="Rhea" id="RHEA-COMP:17428"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:68097"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1X2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004802"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004860.1">
        <property type="protein sequence ID" value="ENSXCOP00000004802.1"/>
        <property type="gene ID" value="ENSXCOG00000003772.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016193"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd07711">
        <property type="entry name" value="MBLAC1-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039344">
        <property type="entry name" value="MBLAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23200">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23200:SF48">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="8">
        <location>
            <begin position="46"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="211" mass="22728" checksum="9DEB078D65BFD5F4" modified="2018-12-05" version="1">MAAVCGQYQKVCLSETQLDFPGQPYSVSVLKVGYCVPQTDGSFRADGTITLIRGVMDILVDTGGPWNREFLLQSLKRRGLEPGDLQLVVGTHGHSDHIGNLNLFPSALTIVGYDISEGDIYRPNQLAEGQIYTVDDHVCVVPTPGHTGQDVSVQVEGTSVGTVLVAGDLFEGWSDDNSWKDLSMNSAVQEVSRQKALNTADVIIPGHGLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L254</accession>
    <name>A0A3B5L254_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Lipoyl-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L254"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006388"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006468.1">
        <property type="protein sequence ID" value="ENSXCOP00000006388.1"/>
        <property type="gene ID" value="ENSXCOG00000004944.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00900000141164"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.600.10:FF:000001">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.600.10">
        <property type="entry name" value="pyruvate carboxylase f1077a mutant domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003379">
        <property type="entry name" value="Carboxylase_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055268">
        <property type="entry name" value="PCB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778">
        <property type="entry name" value="PYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778:SF2">
        <property type="entry name" value="PYRUVATE CARBOXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02436">
        <property type="entry name" value="PYC_OADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89000">
        <property type="entry name" value="post-HMGL domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0092">Biotin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="2">
        <location>
            <begin position="250"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="320" mass="35390" checksum="C2FCF9B43A7BC9E2" modified="2018-12-05" version="1">MVLLYFKATLKLTFAQHFLQHNGLSRGFKSPFKSLKLFNTNTDFFLQVTPSSKIVGDLAQFMVQNNLTRAEVEERADELSFPQSVVEFLQGHVGIPHGGFPEPFRSKVLKSLPRIEGRPGASLSPMDFKTLEDGLRAAHGDDITPEDVMSAAMYPKVFQEFKEFTSNFGPVDCLSTRLFLDGPKIAEEFEVELERGKTLHIKALALGDLNKGGQREVFFELNGQLRSVLVKDTVAMKEMKFHPKAQKSIKGQVGAPMPGKVLEVKVKVGSKVEKGQPLCVLSAMKMETVVNSPVSGTVKAVHVEPEASLEGDDLILEIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2I5</accession>
    <name>A0A3B5L2I5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA2/NAM7 helicase family. SDE3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005012"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005072.1">
        <property type="protein sequence ID" value="ENSXCOP00000005012.1"/>
        <property type="gene ID" value="ENSXCOG00000003930.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156024"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032574">
        <property type="term" value="F:5'-3' RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18038">
        <property type="entry name" value="DEXXQc_Helz-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049080">
        <property type="entry name" value="MOV-10-like_beta-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026122">
        <property type="entry name" value="MOV-10/SDE3_DEXXQ/H-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049079">
        <property type="entry name" value="Mov-10_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45418">
        <property type="entry name" value="CANCER/TESTIS ANTIGEN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45418:SF1">
        <property type="entry name" value="CANCER_TESTIS ANTIGEN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21634">
        <property type="entry name" value="MOV-10_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21635">
        <property type="entry name" value="Mov-10_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0943">RNA-mediated gene silencing</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="280"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="616" mass="70396" checksum="1903E79FF6D55819" modified="2018-12-05" version="1">KETTQKALKTLRATSFFTRWKQNGGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSIKVLYAEVFVVKVSTLRDCRCFHQMTCVSPMPYCQEETVCTYCFIVLESPLSWENYTEKFKLLLYLEELQMEFDIRRYNIPNEDRKFAQLNRDLNNPRLLLLEVPGVSENRPSVLRGDSMLVYPQGEKEVKYRGYVYSVQLDSVRLGFASDLIEGIKFNVEFIINRLTVRLQHRAAEFARIHKLSSVLFPAAPPILFDYQLEKNPEQYQAVQHIVAGSSRPAPYLVFGPPGTGKTVTLVEAIKQIEKTKTFCHILACAPSNSAADLLCSKIREHVDEHKVYRVYASSRDPKLVPEHLKVSACSNLLGDSYIFPAKEELMEFRIIVTTLLTAGRFVSGDIPSGHFTHIFVDEAGHAVETECLVPLAGLLNTETGQLVLAGDPKQLGPILRSPFALKYRMGMSLLERLMTDFPLYQKNDGVYNNCFVTKLLRNYRSHPAILKIPNELFYEGELQACAEEYSRNLYCRWEYLPKPVFILPSKTPHTERSAFRSRGKELINYKGLIVLSCPASQLVSVTWFREKEEREEHQRPPHASPYSDYPTGCKEACHFVDMIEKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2L7</accession>
    <name>A0A3B5L2L7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2L7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002029"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002059.1">
        <property type="protein sequence ID" value="ENSXCOP00000002029.1"/>
        <property type="gene ID" value="ENSXCOG00000001633.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287280"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF18">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017212293" description="Ig-like domain-containing protein" evidence="6">
        <location>
            <begin position="19"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="14"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="115" mass="12588" checksum="7FC951EAA32BF36D" modified="2018-12-05" version="1">LALILGYIILILFPCVNCQQLTAAKTEEFSSEGSSVTLTCSYSKAAVYDDYIFWYRQYPGKPPDFLISHSGTGEKLQDPVPGFSFNMNADKTQMDLLISSAAVTDSAVYYSASSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L524</accession>
    <name>A0A3B5L524_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine-protein phosphatase 4 regulatory subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">SMEK homolog 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Regulatory subunit of serine/threonine-protein phosphatase 4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMEK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L524"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005505"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005568.1">
        <property type="protein sequence ID" value="ENSXCOP00000005505.1"/>
        <property type="gene ID" value="ENSXCOG00000004299.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018199"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030289">
        <property type="term" value="C:protein phosphatase 4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072542">
        <property type="term" value="F:protein phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:DNA damage response"/>
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        <property type="entry name" value="Serine/threonine-protein phosphatase 4 regulatory subunit 3B"/>
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    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055236">
        <property type="entry name" value="EVH1_PP4R3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006887">
        <property type="entry name" value="P4R3-like_central_dom"/>
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        <property type="entry name" value="PH-like_dom_sf"/>
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        <property type="entry name" value="PP4R3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23318">
        <property type="entry name" value="ATP SYNTHASE GAMMA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23318:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EVH1_PP4R3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04802">
        <property type="entry name" value="PP4R3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PP4R3 EVH1-like" evidence="7">
        <location>
            <begin position="5"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Serine/threonine-protein phosphatase 4 regulatory subunit 3-like central" evidence="6">
        <location>
            <begin position="143"/>
            <end position="633"/>
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    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="724"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="727"/>
            <end position="744"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008809"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00042013"/>
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            <dbReference type="ARBA" id="ARBA00057252"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00068937"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Pfam" id="PF04802"/>
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        <source>
            <dbReference type="Pfam" id="PF22972"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="757" mass="88074" checksum="B636622F08C5B45E" modified="2018-12-05" version="1">MTDTRRRVKVYTLNEDRQWDDRGTGHVSSCYVERLKGTSLLVRAESDGSLLLESKINPNTAYQKQQDTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVDITQEVVDESEEERFDDMSSPGLELPPCELNRLEDLAELVASSLPSPLRREKLALAVENEGYIRKLLELFRVCEDLENREGLHQLYEIIKGIFLLNRTALFEVMFSDECIMDVIGCLEFDPALPQPRRHREFLTKTARFKEVIPISDPELRQKIHQTYRVQYIQDMVLPTPSVFEENMLSTLHSFIFFNKVEIVGMLQDDEKFLTDLFAQLTDEATDDDKRQELVNFLKEFCAFSQTLQPQNRDAFFKTLSNMGILPALEVILGMDDVQVRGAATDIFSYLVEYNPSMVREFVMQESQQNDDDILLINLIIEHMICDTDPELGGAVQLMGLLRTLLDPENMLATANKTEKTEFLSFFYKHCMHVLSAPLLANTTEEKPSKDDFQTAQLLALILELLTFCVEHHTYHIKNYIINKDILRRVLVLTASQHAFLALCALRFMRRIVGLKDEFYNRYIMRNFLFEPVVKAFRNNGSRYNLLNSAMIEMFEYVRVEDIKSLTAHIVENYWKSLEDVDYVQTFKGLKLRYEQQRERQDNPKLDSMRSILRNHRFRRDARTLEDEEEMWFNTDEDDLEDGEAVVPPSDKMKSEEDLMEPISKFMERKKCKYQKMALQYGGLVGLVDYPDDDEEEEEEEDGESQEDALPPTKKSKLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L664</accession>
    <name>A0A3B5L664_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-actin-capping protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the F-actin-capping protein alpha subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L664"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006302"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006379.1">
        <property type="protein sequence ID" value="ENSXCOP00000006302.1"/>
        <property type="gene ID" value="ENSXCOG00000004872.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cortical cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:F-actin capping protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:actin cytoskeleton organization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="F-actin-capping protein subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.60">
        <property type="entry name" value="F-actin capping protein, alpha subunit"/>
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        <property type="entry name" value="F-actin capping protein, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002189">
        <property type="entry name" value="CapZ_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037282">
        <property type="entry name" value="CapZ_alpha/beta"/>
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        <property type="entry name" value="CapZ_alpha/beta_2"/>
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        <property type="entry name" value="CapZ_alpha_1"/>
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        <property type="entry name" value="F-actin_cap_asu_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10653">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10653:SF2">
        <property type="entry name" value="F-ACTIN-CAPPING PROTEIN SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F-actin_cap_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00191">
        <property type="entry name" value="FACTINCAPA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90096">
        <property type="entry name" value="Subunits of heterodimeric actin filament capping protein Capz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00748">
        <property type="entry name" value="F_ACTIN_CAPPING_A_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00749">
        <property type="entry name" value="F_ACTIN_CAPPING_A_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0117">Actin capping</keyword>
    <keyword evidence="3 4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010479"/>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="258" mass="29654" checksum="BC2037816D56C29B" modified="2018-12-05" version="1">FNEVFNDVRLLLNNDNLLREGAAHAFAQYNLDQFTPVKVEGYEEQVLITEHGDLGNGRVLDPKNKISFKFDHLRKEASDPRPHDVDGSMEGWRSAVDSAVRAYVKEHYPNGVCTIYGKTIDGHQTIIVCIECHQFQPKNFWNGRWRSEWKFTISPSTTQVAGIMKIQVHYYEDGNVQLVSHKEVQESMSVSNEASTAKEFVKVMEAAENDYQTAINENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6F2</accession>
    <name>A0A3B5L6F2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 2 member 11, like</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6F2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005995"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006063.1">
        <property type="protein sequence ID" value="ENSXCOP00000005995.1"/>
        <property type="gene ID" value="ENSXCOG00000004653.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166787"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046323">
        <property type="term" value="P:D-glucose import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070837">
        <property type="term" value="P:dehydroascorbic acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF54">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="17"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="425" mass="47830" checksum="5F7D19E7A8216607" modified="2018-12-05" version="1">VWCVYLQLNYPVLIAAIFICSIGGTFQYGFSVSVMTSPSQFIKDLVNKSCVERYDRILMDWEVSLIWSFTVSIFCFGGLLGSLVASSISSRFGRKRCLLVNNFWAILGAILMISTRTANSFEMIMVGRFLYGINSGVGLSAQTMYLTESSPKNLRAMVGVTIATALAIGKFCGQLLGIRELLGTEKSWPWLLGFNGFIALFQFCTLPLLPESPRFLLLERGDLQASENAFRKLWGKKDYSKEVVEMLEEKAALQSIRSCSVLELIQNRTVRWQVLTIVIVFFALQFSGINAVYFYSFDVFRAAGIQEHRLAYAALGTGLCELTTSIGCVSPPSLIFTYYLLTFHTCKLIYHFKCFQENLGNFCFLIFLAVCLICGFHVRFNVPETKARTALEVSAEFDRMHAKDKTSQKKNSSKICEIKAQETKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9T2</accession>
    <name>A0A3B5L9T2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Gamma-aminobutyric acid type A receptor subunit beta2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">GABRB2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9T2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007592"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007688.1">
        <property type="protein sequence ID" value="ENSXCOP00000007592.1"/>
        <property type="gene ID" value="ENSXCOG00000005848.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154245"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19053">
        <property type="entry name" value="LGIC_TM_GABAAR_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000004">
        <property type="entry name" value="Gamma-aminobutyric acid receptor subunit beta-2 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000025">
        <property type="entry name" value="Gamma-aminobutyric acid type A receptor beta3 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002289">
        <property type="entry name" value="GABAAb_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01160">
        <property type="entry name" value="GABAARBETA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="270"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="418"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="43"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="212"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="437" mass="50765" checksum="8294413E94A7A433" modified="2018-12-05" version="1">PFRMPENMDLLQESKVHMSVDHRCPGIKSGTNPLIIRWSQCFDYTLTMYFQQAWRDKRLSYSEISLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHPDGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGGNNAVTGVDKIELPQFSIVDHKLISKNVVFSTGSYPRLSLSFKLKRNIGYFILQTYMPSILITILSWVSFWINYDASAARVALGITTVLTMTTINTHLRETLPKIPYVKAIDMYLMGCFVFVFLALLEYALVNYIFFGQGPQRQKKAAEKTATANNEKMRMDPNKMDTHENILLGTLDIKNDMGVTELALGLNDPRNTMLTYDSSNLQYRKSGLTRHNFGRNALERHMTQKKSRLRRRASQLKITIPDLTDVNSIDKWSRMIFPTVFSFFNIVYWLYYVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBD8</accession>
    <name>A0A3B5LBD8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the POU transcription factor family. Class-3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBD8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009508"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009620.1">
        <property type="protein sequence ID" value="ENSXCOP00000009508.1"/>
        <property type="gene ID" value="ENSXCOG00000007239.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164385"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000005">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000001">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016362">
        <property type="entry name" value="TF_POU_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF115">
        <property type="entry name" value="POU DOMAIN, CLASS 3, TRANSCRIPTION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002629">
        <property type="entry name" value="Transcription_factor_POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="14">
        <location>
            <begin position="212"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="302"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="304"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="95"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="160"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="361"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="104"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="175"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="390" mass="42799" checksum="F861C07A3A2F469B" modified="2018-12-05" version="1">MATAASNHYSVLSTPSSAPPPPPHSESGSMQQAAAYRDAHTLLQNDYGTLPGGGHPLSHAHQWITALSHGDSGTPWPSSPLREQDVKPILHDSDREELQNSSSLQQQQQQQQQQQRHPHLAHQQAHHDARAWRTSTATTHITGMATSEGQSLVYTQSGFALMPGGEQGGMHHHPLRDEDHHSHSPHLSEHGGGPGAHHHHQQHGGHQDQSDEDTPTSDELEQFAKQFKQRRIKLGFTQADVGLALGTLYGNVFSQTTICRFEALQLSFKNMCKLKPLLNKWLEEADSTSGSPTSLDKIAAQGRKRKKRTSIEVGVKGALESHFLKCPKPGAAEINSLADSLQLEKEVVRVWFCNRRQKEKRMTPAGGQISGGEDMYGDTPPHHGGQTPVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LC76</accession>
    <name>A0A3B5LC76_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LC76"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005474"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005536.1">
        <property type="protein sequence ID" value="ENSXCOP00000005474.1"/>
        <property type="gene ID" value="ENSXCOG00000004273.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165011"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01252">
        <property type="entry name" value="PH_GRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000309">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051707">
        <property type="entry name" value="PI-Interact_SigTrans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14336">
        <property type="entry name" value="TANDEM PH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="44"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="181" mass="20993" checksum="658869011479D65B" modified="2018-12-05" version="1">MNRGINNGADLPVDLLTKLYASIRSEPFKIPEDDGNDLTLTFFNPDREGWLLKMGGRVKTWKRRWFILTESCLYYFQYTTDKDPIGIIPLENLCVRKLQDSSKPFCLELYSPKGQKVKACKTENKGRVVEGKHQFYRLNAASEEERDDWISAIRASITKDPFYDLVSMRKRKVIRNASSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCG5</accession>
    <name>A0A3B5LCG5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sorting nexin-3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Phagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027896373.1">
        <property type="nucleotide sequence ID" value="XM_028040572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCG5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005559"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005625.1">
        <property type="protein sequence ID" value="ENSXCOP00000005559.1"/>
        <property type="gene ID" value="ENSXCOG00000004336.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114158750"/>
    <dbReference type="KEGG" id="xco:114158750"/>
    <dbReference type="CTD" id="8724"/>
    <dbReference type="GeneTree" id="ENSGT00940000153609"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030218">
        <property type="term" value="P:erythrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042541">
        <property type="term" value="P:hemoglobin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034499">
        <property type="term" value="P:late endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051246">
        <property type="term" value="P:regulation of protein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051223">
        <property type="term" value="P:regulation of protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07293">
        <property type="entry name" value="PX_SNX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000002">
        <property type="entry name" value="Sorting nexin 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042137">
        <property type="entry name" value="PX_SNX3_Vert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051074">
        <property type="entry name" value="Sorting_Nexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45963">
        <property type="entry name" value="RE52028P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45963:SF1">
        <property type="entry name" value="SORTING NEXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="13" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="PX" evidence="15">
        <location>
            <begin position="27"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="162" mass="18774" checksum="C3AD3BE5BF199BFB" modified="2018-12-05" version="1">MADAIADTRRLYTKPQNLNDAYGPPSNFLEIDVSNPETVGVGRGRYTTYEVRLKTNLPIFKLKESSVRRRYSDFEWLRAELERESKVVVPPLPGKALFRQLPFRGDDGIFDDSFIEERRQGLEQFLNKVAGHPLAQNERCLHMFLQDEIVDKNYTPSKIRQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDC6</accession>
    <name>A0A3B5LDC6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G protein-coupled receptor 158b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDC6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010173"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010292.1">
        <property type="protein sequence ID" value="ENSXCOP00000010173.1"/>
        <property type="gene ID" value="ENSXCOG00000007716.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155918"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043458">
        <property type="entry name" value="GPR158/179"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32546:SF27">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 158B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32546">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 158-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017299665" description="G protein-coupled receptor 158b" evidence="9">
        <location>
            <begin position="18"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="216"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="417" mass="46572" checksum="8C5CD04550F37D43" modified="2018-12-05" version="1">MALILFALLLHTGLVLGSNYGYVEWSDNDRDEKHPPAAFSTQSVNKEPPHRPTPHPLQPTERAVVAHKIEEDLPRVVTAFLHTGDSSALRQVNCSRRYELSSLRGGLHVASHYSLHSVLDTVAHATNFLNMILQTNRSRERTFRGDTGWYHALVQSILEGDPKIHRAVVTLHADAPTTPDPHVFLQATRTGNEVVLQDLSGSAHRRLKNRSPESDWFHDFRDGRRPQMHSGVPEGSGTGGGSYLLNKSQIKWSAPYLECEHGAFVPYWLLTLSAGFYGLKSNSCTPVGGHGFVLDKYKCLCRKGFYHPSRVALNGFKSRLQISFSYRDETSDVSNKCLPCREGCKYCQDDTPCLVQEDGGLRLAVASFQGLCMMLDLASMLVVYHFRRNKHPYFWSHPAGSHLVWGVAAVLPSKSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDG2</accession>
    <name>A0A3B5LDG2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Post-glycosylphosphatidylinositol attachment to proteins 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDG2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010208"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010330.1">
        <property type="protein sequence ID" value="ENSXCOP00000010208.1"/>
        <property type="gene ID" value="ENSXCOG00000007742.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160060"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021910">
        <property type="entry name" value="NGX6/PGAP6/MYMK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14319">
        <property type="entry name" value="FIVE-SPAN TRANSMEMBRANE PROTEIN M83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14319:SF7">
        <property type="entry name" value="POST-GPI ATTACHMENT TO PROTEINS FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12036">
        <property type="entry name" value="DUF3522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="361"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="447"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8 9">
        <location>
            <begin position="250"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS01186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="511" mass="56596" checksum="40453B5E69908A71" modified="2018-12-05" version="1">LSELPQHGVLLFSKWCLINSDVLNRGRLFVPEFTSSLSISVAECSSGEASESAGCSLLLRLGSATLQHGQTELNCSGVSCSVSLSNPPWDSWLRVVVEIKNVNSMIAVTFSGSNVGKKPHQGSISNATATGNGSAPAPPASACVWSVPVLYEELDVLSLRYTPVNGSNVSVTETHPTLLTYPLPPQATGGTLNLQLTVPKAVVRLPFPQSTTWYLTLHIMTCFSLACVDDCGPYGECRLLRSYTYLYSACVCSAGWKGWGCTDGSTAQSYARQLTATMLLTLSNLFFLPAIVVAIRRSYITEAFVYLFTMSFSTFYHACDQPGAVVLCIMDYDALQYCDFLGSICSIWVTILCMARLGDTAKYTLFLLGALLIAMSMQLDRKGLWNLLGPILCAILLMVTSWVYRGVRRRHCYPPSWRRWVFYLLPGALCALIGVCLYVFAETESNYYYTHSLWHILVASCVVFLLPPREKDRKALGWSRGLSWSWRWSWRPRVCGYTLCESSKDELYTVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEF9</accession>
    <name>A0A3B5LEF9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LEF9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009227"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009338.1">
        <property type="protein sequence ID" value="ENSXCOP00000009227.1"/>
        <property type="gene ID" value="ENSXCOG00000006997.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234570"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000019">
        <property type="entry name" value="Myosin light chain 1 skeletal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF30">
        <property type="entry name" value="CARDIAC MYOSIN LIGHT CHAIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="88"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="155" mass="17276" checksum="B7472C4437A6AF75" modified="2018-12-05" version="1">IVVVEFSPDQVEEFRDAFSLFDETPGGEMKISYAQCGDVLRALGHNPTNDEVLKLLGRPKAEEMHASLLDFQAFLPMLQHVARAKEQGNMEDFVEGLRVFDKEGNGTVMGAELRHVLATLGERMTEDEVDRLMAGQEDANGHVNYTEFVKHILAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LF63</accession>
    <name>A0A3B5LF63_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">N-terminal E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
            <ecNumber evidence="10">2.3.2.25</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrate and DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N-terminal-amino acid = [E1 ubiquitin-activating enzyme]-L-cysteine + N-terminal-ubiquitinyl-[acceptor protein].</text>
            <dbReference type="EC" id="2.3.2.25"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="EC" id="2.3.2.25" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LF63"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009215"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009326.1">
        <property type="protein sequence ID" value="ENSXCOP00000009215.1"/>
        <property type="gene ID" value="ENSXCOG00000007028.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156908"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23808">
        <property type="entry name" value="UBCc_UBE2W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000022">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="13">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="141" mass="16204" checksum="871FEB4AC939278C" modified="2018-12-05" version="1">KRLQKELLALQNDPPPGMTLNEKSVQNTITWIVDMEGAPGTLYEGEKFQLLFKFSGRYPFDSPQVMFTGENIPVHPHVYSNGHICLSILTEDWSPALSVQSVCLSIISMLSSCKEKRRPPDNSFYVRTCNKNPKKTKWWYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFB7</accession>
    <name>A0A3B5LFB7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFB7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008381"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008487.1">
        <property type="protein sequence ID" value="ENSXCOP00000008381.1"/>
        <property type="gene ID" value="ENSXCOG00000006425.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164867"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097120">
        <property type="term" value="P:receptor localization to synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099072">
        <property type="term" value="P:regulation of postsynaptic membrane neurotransmitter receptor levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06723">
        <property type="entry name" value="PDZ1_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06724">
        <property type="entry name" value="PDZ2_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000049">
        <property type="entry name" value="disks large homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000002">
        <property type="entry name" value="Disks large homolog 4 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019583">
        <property type="entry name" value="DLG1-4_PDZ_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019590">
        <property type="entry name" value="DLG1_PEST_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050614">
        <property type="entry name" value="Synaptic_Scaffolding_LAP-MAGUK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119">
        <property type="entry name" value="DISCS LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119:SF5">
        <property type="entry name" value="DISKS LARGE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10608">
        <property type="entry name" value="MAGUK_N_PEST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10600">
        <property type="entry name" value="PDZ_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01277">
        <property type="entry name" value="MAGUK_N_PEST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="157"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="252"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="387"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="47"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="343"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="80"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="523" mass="56332" checksum="C111B49140CA89BC" modified="2018-12-05" version="1">MPVRQKDAQRALELLQHRIINVFQSQLFSALLGKNILDGGTLGRDGGTLGRSGGTLGRQGGTLGRDRGTWRRGGGGIGSMERERDRSLGRDGSLGHHGDLRGAELVQVAERQLSHIQHVHGYVTHTHIAPTQVTRCGTLCDFSSYVNGTEADYEYEEITLERGNSGLGFSIAGGTDNPHIGEDPSIFITKIIPGGAAAQNGRLRVNDCIVRVNDTDVREVTHSGAVEALKEAGGLVRLCIRRRRSLAERIMDIKLVKGPKGLGFSIAGGVGNQHVPGDNSIYVTKVIEGGAAHKDGRLQIGDKLVAVNASCLEEVTHEEAVAALKSTPDVVYLRVSKHTYSPHQDNHVSPYMSGSQSVSPAPLTTPRYSPLPRSLTTDEDVPREPRRVVLQRGSTGLGFNIVGGEDGEGIFISFILAGGPADLCGELRKGDRILSVRTKISSRTVLVSIIIYNGNNSTFGRNDSSNDSSTSIIKNPRGKYICLEALVNYVLLCSAAFRPDHHLWRAALLFPPMSCCEVLAARC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGV7</accession>
    <name>A0A3B5LGV7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGV7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009943.1">
        <property type="protein sequence ID" value="ENSXCOP00000009825.1"/>
        <property type="gene ID" value="ENSXCOG00000007459.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234555"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001609">
        <property type="term" value="F:G protein-coupled adenosine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001634">
        <property type="entry name" value="Adenosn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24246:SF54">
        <property type="entry name" value="ADENOSINE RECEPTOR A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24246">
        <property type="entry name" value="OLFACTORY RECEPTOR AND ADENOSINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00424">
        <property type="entry name" value="ADENOSINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="31"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU201114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="329" mass="36445" checksum="5E78B45A1746E710" modified="2018-12-05" version="1">QGANSTSMMADWAWVTYTVLEVLIAVACCLGNVLVVCAVAVGIHDCFREPTFCFLVSLAVADFLVGAAAVPLAVLLDGWVNLTSHQCLFLSCVILVLTEASVLSLLAIAIDRYLRLHMPFRYKAIVTQRRTLLAMSICWILSFLLGFTPLLGWHNLSAAGHYSTNTSSTLPSSSQCTFLSVISLPFMVYFNFLGCIMAPLLVMTLLYIIIFWSLHKRLKNSCPQAQALLLREKKLACSLALVLTLFAFCWIPLHLMNCLLFLWGPQAVTQGTLYTGILLSHANSAVNPVVYACRIPKIQKAYCQIWKHLALKLNCFTRNESIQQAITSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LI19</accession>
    <name>A0A3B5LI19_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LRRNT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LI19"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011773"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011909.1">
        <property type="protein sequence ID" value="ENSXCOP00000011773.1"/>
        <property type="gene ID" value="ENSXCOG00000008897.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
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        <property type="entry name" value="LRR_dom_sf"/>
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        <property type="entry name" value="LRR_TM_domain-containing"/>
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        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF213">
        <property type="entry name" value="INSULIN LIKE GROWTH FACTOR BINDING PROTEIN ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="3"/>
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    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
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    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017255571" description="LRRNT domain-containing protein" evidence="4">
        <location>
            <begin position="44"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="5">
        <location>
            <begin position="43"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="332" mass="36923" checksum="2B6118232A1929FF" modified="2018-12-05" version="1">FFFYVTLFGLGSWELWGGDSCCHPAVVAMLLLLCLGFVPTVAPCPPHCLCASDIISCSGSNLSALPFGLPSYATRLDLSHNALRTVQVAWVSQPFQRLTTLVLSWNSISQIEMAAFTATPRLLHLDLSSNQLRVLNSSVFSGLTELKELLLIGNQIAQIMPGAFSDVNSLQKLYLSANRLTSFPLGLFEDPSGPRNLTFLDLSYNRLSKLPENPLLCDCALIALLEYWLWKQYRPLVDFQVGYQCRGTAVREVNWLIIFWVTPRTALNSSASDLRSHLSVFPNGTLEIQGALMEDSGIYECNLNSVYSLCGVCFIFRVHMEMSQLSLRIVHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LID0</accession>
    <name>A0A3B5LID0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Polypyrimidine tract-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LID0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010627"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010751.1">
        <property type="protein sequence ID" value="ENSXCOP00000010627.1"/>
        <property type="gene ID" value="ENSXCOG00000008030.1"/>
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    <dbReference type="GeneTree" id="ENSGT01050000244924"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903312">
        <property type="term" value="P:negative regulation of mRNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12693">
        <property type="entry name" value="RRM2_PTBP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12695">
        <property type="entry name" value="RRM3_PTBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000036">
        <property type="entry name" value="polypyrimidine tract-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000162">
        <property type="entry name" value="polypyrimidine tract-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000018">
        <property type="entry name" value="Polypyrimidine tract-binding protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000032">
        <property type="entry name" value="Polypyrimidine tract-binding protein 2 isoform 1"/>
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    </dbReference>
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        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006536">
        <property type="entry name" value="HnRNP-L/PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055204">
        <property type="entry name" value="HNRNPL_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021790">
        <property type="entry name" value="PTBP1-like_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035001">
        <property type="entry name" value="PTBP1_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01649">
        <property type="entry name" value="hnRNP-L_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15592">
        <property type="entry name" value="MATRIN 3/NUCLEAR PROTEIN 220-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22976">
        <property type="entry name" value="RRM_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13893">
        <property type="entry name" value="RRM_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11835">
        <property type="entry name" value="RRM_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="9 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="56"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="167"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="345"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="462"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="539" mass="58843" checksum="75B6238A5A2AF372" modified="2018-12-05" version="1">MSGIIPDKLMLGRKLRGSDELFSSVSNGPYIMSTTANGNDSKKFKGDIRGPSLPSRVIHIRKLPSDITEAEVISLGLPFGDVTNLLMLKAKNQAFLEMNSEEAAQNMVGYYSTMMPVIRHQPVYLQFSNHKELKTDNSPNQERAQAALRALSSSHVDTLMAAPSTVLRVVVENLLYPVTLDALCQIFSKFGTVLRIIIFTKNNQFQALLQFSDGSSAQAAKLTLDGQNIYNGCCTLRISFSKLTSLNVKYNNEKSRDFTRPDLPSGDSQPALEHPAMAAAFAPGIISAAPYAGATHGFPPAFAFQPAVSSAYPGLTLPALPGALASLGLPATARLGFPSIPAGHSVLLVSNLNPERVTPHCLFILFGVYGDVMRVKILFNKKENALVQMCDSTQAQLAMSHLNGQRLHGKPLRITLSKHTSVQLPREGHEDQGLTKDYSNSPLHRFKKPGSKNYSNIFPPSATLHLSNIPPSVVEDDLKMLFSSSGAIVKAFKFFQKDHKMALIQMGSVEEAIDCLITFHNHDLGETHHLRVSFSKSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIU4</accession>
    <name>A0A3B5LIU4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LysM and putative peptidoglycan-binding domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027868176.1">
        <property type="nucleotide sequence ID" value="XM_028012375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIU4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007794"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007892.1">
        <property type="protein sequence ID" value="ENSXCOP00000007794.1"/>
        <property type="gene ID" value="ENSXCOG00000005985.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114141681"/>
    <dbReference type="KEGG" id="xco:114141681"/>
    <dbReference type="CTD" id="388695"/>
    <dbReference type="GeneTree" id="ENSGT00940000160002"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045030">
        <property type="entry name" value="LYSM1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20932:SF2">
        <property type="entry name" value="AND PUTATIVE PEPTIDOGLYCAN-BINDING DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20932">
        <property type="entry name" value="LYSM AND PUTATIVE PEPTIDOGLYCAN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="LysM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="3">
        <location>
            <begin position="40"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="93"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00040996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="213" mass="23243" checksum="F19F5A944BAD9DE4" modified="2018-12-05" version="1">MSGERASLPSGGGGLLRGSRTRSYGSLTSSSLSPVRQKVIEHTVKPGETLQGLALKYGVTVELIKRANRLYTNDSIFLKKSLSIPVLSDLNDHSNGVDSSEEDHKQGKTNCDTEDYRKPASDLTPGDFLKRLDNLISQSKEAAARGCKDAEKRVAAVEAACSSTTSDWRPLTRSQSVISSSRFQQQAQLGAVPLTTTKLTKKLKEREDEIFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJ26</accession>
    <name>A0A3B5LJ26_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Proto-oncogene tyrosine-protein kinase receptor Ret</fullName>
            <ecNumber evidence="14">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation in response to glia cell line-derived growth family factors (GDNF, NRTN, ARTN, PSPN and GDF15). In contrast to most receptor tyrosine kinases, RET requires not only its cognate ligands but also coreceptors, for activation. GDNF ligands (GDNF, NRTN, ARTN, PSPN and GDF15) first bind their corresponding GDNFR coreceptors (GFRA1, GFRA2, GFRA3, GFRA4 and GFRAL, respectively), triggering RET autophosphorylation and activation, leading to activation of downstream signaling pathways, including the MAPK- and AKT-signaling pathways. Acts as a dependence receptor via the GDNF-GFRA1 signaling: in the presence of the ligand GDNF in somatotrophs within pituitary, promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Required for the molecular mechanisms orchestration during intestine organogenesis via the ARTN-GFRA3 signaling: involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Mediates, through interaction with GDF15-receptor GFRAL, GDF15-induced cell-signaling in the brainstem which triggers an aversive response, characterized by nausea, vomiting, and/or loss of appetite in response to various stresses. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Also active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Involved in the development of the neural crest. Regulates nociceptor survival and size. Phosphorylates PTK2/FAK1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 24">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJ26"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007884"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007982.1">
        <property type="protein sequence ID" value="ENSXCOP00000007884.1"/>
        <property type="gene ID" value="ENSXCOG00000006056.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158499"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05045">
        <property type="entry name" value="PTKc_RET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000190">
        <property type="entry name" value="Proto-oncogene tyrosine-protein kinase receptor Ret"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000234">
        <property type="entry name" value="Proto-oncogene tyrosine-protein kinase receptor Ret"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041163">
        <property type="entry name" value="Ret_CLD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040667">
        <property type="entry name" value="Ret_CLD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041317">
        <property type="entry name" value="RET_CLD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055162">
        <property type="entry name" value="RET_CRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016249">
        <property type="entry name" value="Tyr_kinase_Ret_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF485">
        <property type="entry name" value="PROTO-ONCOGENE TYROSINE-PROTEIN KINASE RECEPTOR RET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17756">
        <property type="entry name" value="RET_CLD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17812">
        <property type="entry name" value="RET_CLD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17813">
        <property type="entry name" value="RET_CLD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22540">
        <property type="entry name" value="RET_CRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000631">
        <property type="entry name" value="TyrPK_receptor_Ret"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0106">Calcium</keyword>
    <keyword evidence="14" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10 14" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="21">
        <location>
            <begin position="185"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="697"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="847"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="731"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="16">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR000631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000631-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000631-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1087" mass="122120" checksum="0A9F0364EE323475" modified="2018-12-05" version="1">PGSSSGYFIIWFFLLPAGVAGLYFPQKEYTETVYVGQPAGTPVLQVHAMLDGRSERPHFYLCGSRRPPYALWFQMDVQTGELSLSKTLEETDFLRSGSNSWPAKRLTLQVMALNSVSKRAPCSRNSAHITLDFVNGSMPRCAQTDMKELCFPHRDTSNPHIIENRFPGVLRQLRRLTRLTVCPNYTIAYGVESETPAPFAVNGNTSELVVTAPLDREESENYRLLLVCTVQTESVVTKVETPLDVFVDDEDDNAPYVNGTDTVFVLVFQGAAFGTLFVFDRDLTTLFPKDLSHNRYVGTLLSSESWIKDTFDIKGTFSERRASPGGIRESVYDYQLILKRNLDITENRTVFLDYLVNDTTYPGLEGTVLLHFNVTILPVQIRFANITHVFTLSRKASIYAQVGRICVENCLEFDGFSVSYQLEVPDKNMSSELLSCYSAISITQAPDRMLGLLYVNNSGALSKPGCQDLDYTVVAQEEQTQLVASTQIPNVSQESQQLLSCAENRQRDDCEASQGLGATTGACQWRQGSEKGISENYSTCSPDLRTCPDSVCDAVESRDTSICPQDCTTESVTGGHERGLSGSGIRAGYGTCYCFSEKCFWFPDVICDDMCKTIVATALLCCFIISVLLSSYFIHRYHKNSPKAPIAFAEMTFRRPAQSYPISFPANNVRRGSQESIEADTFKIPEDPKWEFPRKNLVLGKTLGEGEFGKVVKATAFRLKGKAGYTTVAVKMLKENASHTELRDLLSEFTLLKQVNHPHVIKMYGACSQDGPLYLIVEYAKFGSLRNFLRESRKVGPSYMGRDANRNSSYLENPDDRALTMGDLISFAWQISRGMQYLAEMKLVHRDLAARNVLVAEGRKMKISDFGLSRDVYEEDSYVKRSKGRIPVKWMAIESLFDHIYTTQSDVWSFGVLLWEIVTLGGNPYPGIAPERLFNLLKTGYRMERPENCSEEMYNLMLRCWKQEPDKRPTFSDISKELEKMMVKSRDYLDLAASTPADALLYDDALSEEDTPLVDCNNAPLPRTLPSTWIENKLYGMSYPSWPETSPVPLNRHDATNPVFTRYANDSVYANWMALPSPAKAVDKLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJF1</accession>
    <name>A0A3B5LJF1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Sortilin related VPS10 domain containing receptor 3b</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS10-related sortilin family. SORCS subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJF1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009741"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009856.1">
        <property type="protein sequence ID" value="ENSXCOP00000009741.1"/>
        <property type="gene ID" value="ENSXCOG00000007222.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234563"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00146">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000083">
        <property type="entry name" value="Sortilin-related VPS10 domain containing receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.80:FF:000001">
        <property type="entry name" value="Sortilin-related VPS10 domain-containing receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031777">
        <property type="entry name" value="Sortilin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031778">
        <property type="entry name" value="Sortilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006581">
        <property type="entry name" value="VPS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050310">
        <property type="entry name" value="VPS10-sortilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106">
        <property type="entry name" value="SORTILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106:SF10">
        <property type="entry name" value="VPS10 DOMAIN-CONTAINING RECEPTOR SORCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00801">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15902">
        <property type="entry name" value="Sortilin-Vps10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15901">
        <property type="entry name" value="Sortilin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00602">
        <property type="entry name" value="VPS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110296">
        <property type="entry name" value="Oligoxyloglucan reducing end-specific cellobiohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="PKD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017366642" evidence="11">
        <location>
            <begin position="26"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1128"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="12">
        <location>
            <begin position="858"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="161"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="169"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="180"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1249" mass="141965" checksum="862C4ECF034C43EC" modified="2018-12-05" version="1">MTLWSFWSCFLLAFIQLFPFHLKLATRAEISFPRLANRLYPMEVTSPMGHMIRKSAGIKVSLKVNDRNEVLHMSETDGPPRPKYKQEVKNETVITNGSTVNNFSTLKIPDKKCDNISTAVIETPKATFSIGERKKRSVGVSYNEHLDRSITGEARRSVRTVQGASNFRSEYRRTGEESRGSNPKQSEPHLDTSTFALSGDSTHNQAMVHWSGQNSSVILILTKLYDLTMGTVTESSLWRSTDYGSTYTKLNDKVTSRTVLSYLYVCPTNKQKILMLSDPEVESTVLISSDEGASFEKFPINFNVLSLLFHPAQEYWILAYSHDYKLYSSMDFGKKWQLVHENVMPGRFYWAVMGLDRESDVVHIETRIAKGRESQYVKCRAQRCTEGDRQYLFPGHIDTNSLVVQDEYVFTQVTKSGRASYFVSYMRESFRQMKLPKYCLPKDMHIISTDEKQVFAAVQEWNQNNTYSLYISDSPGVYFILSLENLRTSRGPAGNLLVDFYKVEGINGMYLANKIVDNHIKTFITYNKGQTWALLPAPATDVSGKNLHCILPFCSLHLHLEMSENPYTSGPITSKKSVPGIIVATGNIGNELSSTNLGMFITSDAGNSWRQIFDEEYNIWFLDNGGALLAVLHAAMPIRQLCLDEGKKWDRHSFSLVPLYVDGVLMEPESDNHIITFFGHFSHRSEWQLIKIDYKGLFSRRCMDGDYQTWSLHNKEEQCVMGEKQKYMKRRPGNRCMLAQDYSRIYSSEPCPCNSYDFECDYGWERQADGKCSPAFWFNPNGVAKSCSSSQSFLNSTGYRKVLSNNCKEEGRNVYLPKRVACQPKSPQGLHLSTSHGELSAAIESNVTFMIHLDNGESPSTSIQLDFGDGIKITYSNLSRTDDGIKHIYRKTGIYRVIATAENNLGSDSSALFLHITSPVDRVYLSAPIVAIREKAANLTAVVWPSHTRTLTFFWWFENSSEPIITLEGSISHMFLREGENKVTVQVACGSSVLEDSKVITVKEFFRSLLLSFSPALDEHNPDIPEWREDIGRVVRTALSQVHQLMVSLYPGLPTTAELFILSDEQMSSEHKKRSEEALDTISDIFVTALNQGLVHFELKANIHIVVSMTQQTLAPLVDSNSIHSGPAMLMLISVVFVGIAAFFIYKFKRKIPWIHVETEDPHEKDPEVISTVGQNDAMSKVKLSEFPSQKELMDKELEGRSRGNQFQTFTICSFVQHTVLKHYWKKYRDQFLLQFKIYSNDECHKINT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LK76</accession>
    <name>A0A3B5LK76_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Semaphorin 6A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SEMA6A</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LK76"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011317"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011448.1">
        <property type="protein sequence ID" value="ENSXCOP00000011317.1"/>
        <property type="gene ID" value="ENSXCOG00000008540.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156565"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001224">
        <property type="term" value="P:positive regulation of neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000028">
        <property type="entry name" value="semaphorin-6A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF12">
        <property type="entry name" value="SEMAPHORIN-6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017315298" evidence="15">
        <location>
            <begin position="19"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="16">
        <location>
            <begin position="24"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="346"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="714"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="822"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="871"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="872"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="915"/>
            <end position="928"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="970" mass="108307" checksum="A4C9B76E1325E622" modified="2018-12-05" version="1">MRSEALVLYFTLLQIAGAGFPEDSEPISISHGNYTKQYPAFVGHKPGRNNTQRHKLDIQLILIMNRTLYIAARDHIYTVDTETANGDEIFFSKKMTWKSRQADVDTCRMKGKHKDECHNFIKVLLQQNGDTLFVCGTNAFNPSCRTYKMDSLEPLGEEISGMARCPYDAKHANVALFADGKLYSATVTDFLAIDAVIYRSLGDSPTLRTVKHDSKWLKEPYFVQAVNYGDFIYFFFREIAMEYNTMGKVVFPRVARVCKNDRGGSPRVLEKQWTSFLKSRLNCSIPGDSHFYFNILQAVTDVIHINGRDVVMATFSTPYNSIPGSAVCAYDMAEVARAFTGRFKEQKSPDSTWTPFPEERVPKPRPGSCAGTPSMERYKVSNEFPDDTLNFIKMHPLMDEAVPSIAHRPWFLKTMVRYRLTRIVVDNEAGPHRNHTVVFLGSEKGIILKFLAKMNKGFLNDSLFLEELNVYNPDKCSIEGVEDKRIIGIQIDSKSNALWVAFTSCVVKVPLSRCERHGRCKKSCIASRDPYCGWVSEGACRQVTTNTKSAFEQDVEHGNTDGLGDCQSFVFFEKVSHFALLSDFCLSMCLSAGVIRESYQKGRDQLVPVTLLAIAVILAFAMGAIFSGLIVYCVCDHRRRDLDLTGRKEKDSLHLHSRRGSMNSVTKLTGLFETQAKDGRPEAILTPLMHNGRLTPNAKMLIKADQHHLDLAALPTPESTPMQPRRKPSRGSREWERNQNLINACTKDMPPIGSPVISTDLPLRASPGHIPSVVVLPLPQHQHQLPPHLQQSYQHEYVEQPHHSDLSMAHGVMEDQGATLEYKPGKSHMMDPDGMLPPRVPQREASLTVPPAVPQMGKRLEVHSSVYGLRKGSASSASGSSALKKHNTNSSNSSHLSRHHSFRVETPPPAPQRVDSIQMTTQGISLSRQPGMITYGSLPRTGSKHLKPDVPPKPSVVSLSTKAKSSDSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKH5</accession>
    <name>A0A3B5LKH5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Nuclear inhibitor of protein phosphatase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Protein phosphatase 1 regulatory inhibitor subunit 8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027865404.1">
        <property type="nucleotide sequence ID" value="XM_028009603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKH5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011160"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011288.1">
        <property type="protein sequence ID" value="ENSXCOP00000011160.1"/>
        <property type="gene ID" value="ENSXCOG00000008435.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114139577"/>
    <dbReference type="KEGG" id="xco:114139577"/>
    <dbReference type="CTD" id="799896"/>
    <dbReference type="GeneTree" id="ENSGT00940000156115"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22674">
        <property type="entry name" value="FHA_PPP1R8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000012">
        <property type="entry name" value="Nuclear inhibitor of protein phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050923">
        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23308">
        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FHA" evidence="15">
        <location>
            <begin position="48"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="266"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="349" mass="38723" checksum="D80EA49082B95D44" modified="2018-12-05" version="1">MASKTSKDGPPPFDCPSWAGKPPTGLHLDVMKGDKLIEKLIIDEKKFYLFGRNPDWCDFTIDHQSCSRVHSALVYHKHLKRVFLIDLNSTHGTFLGHIRLEPHKPQQVPIDSTISFGASTRTYTIREKPQTQGTAGTGDSKLGDEDELKGLLGLPEEETELENLTEFNTAHNKRISIMTIEEGNLEIQRPKRKRKNSRVTFSEEDSIINPEDIDPSVGRFRNMVQTAVIPMKKRRFDSQNTLGFDDLASKRIHGYSLGGGLYGDLPPTSHENKPTGAPGGATMQGGLPLPFPNPAPEVDLAPEAPQPPITLNPTPVTAPYLPETVNEPRKKKYAKEAWPGKKPTPSLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKM0</accession>
    <name>A0A3B5LKM0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKM0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012618"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012770.1">
        <property type="protein sequence ID" value="ENSXCOP00000012618.1"/>
        <property type="gene ID" value="ENSXCOG00000009539.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017669"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045022">
        <property type="term" value="P:early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043271">
        <property type="term" value="P:negative regulation of monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006622">
        <property type="term" value="P:protein targeting to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051837">
        <property type="entry name" value="SortingNexin/PXDomain-PKLike"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999:SF40">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN KINASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22999">
        <property type="entry name" value="PX SERINE/THREONINE KINASE PXK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="5">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="86"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="432"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="435"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="445"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="465" mass="52498" checksum="BDB736B0B350EEAC" modified="2018-12-05" version="1">METEKIFSVIYNFFLRKERDGKKSVEADRMCFLQVIRRYSDFDILNSSLMVCGISLPLPPKKLIGNMDREFIAERQRGLQAYLDSITQHPLICTSLFVKKFLDPNNYSANYTEIALQQVSMFFRSDPKWEVLEPLKDMGWRIRKKYFLIKNKEQPKERFLLSWVSRTPDSSGTPYLSPLLFSSTSESSALLVRPFGERGSLRDQICKVKPKESHLKKYCNPKKSQGLELEQIQLFGRQILESLKLLHDSGLFFGHLHASNVMVDDGVCRLVDVENGMLGVPSVLRPAFTQLRKINTTESIDVFCFGHLLYEMTYGRPPDSVPLDQFPSAPHAAVVSVLQSILSADACKSGMPTVPALIQTPNLTESVLRQIPSRLKEVLKSAKGSLEKRLHEEQRMLHQHRRLTRAQSHYGSEEEKKRRKILARKVKHGAAVFLSPPGSGASSPPTCPSSPTPPPATGTPVSLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLH7</accession>
    <name>A0A3B5LLH7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLH7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011923.1">
        <property type="protein sequence ID" value="ENSXCOP00000011787.1"/>
        <property type="gene ID" value="ENSXCOG00000008908.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154446"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000966">
        <property type="entry name" value="ZFP90 zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001450">
        <property type="entry name" value="zinc finger protein 774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 208-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="253"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="281"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="309"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="363"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="30"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="59"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="470" mass="52403" checksum="2991B602C811DA20" modified="2018-12-05" version="1">MFGGPESSTRLSRVQLPDLTIFALVVKEEVPEMSSSSLDQQNLTCSQVKKEEEEQWISHSEKNEVDEKPQLAEVHHIKSEDSRQTETPTSSSAEQMKTESNGEISGGTEPNREPNPNPNLLTNIEERHSASSETEVSDEKEEKNLSGSGSENEDNDESEDDNISGSGSENEDSDESEDDNISGSGSENEDSDESEDDNISDSGSENEDWREPRTRQSGVNSKVGREKSSSCSVEKNHSRGKQNSDAQMGRKSFSCDDCGKIFQKLTALKSHMKIHRSKKLVVCKECGKSFRSQFQLKNHLTLHTGERPFACDKCGKSRKIHLKIHMTIHKGEKPFACIDCGRCFRLKSDLKGHMTTHLDVKPFICGYCGVRFTRKASLCLHSSAPLPTHPHPACSVFVLLLLEPFFAHLPNLQMMDLVNWSINAIDHIFSTRRLGTGEPSCPTGWIHPGFIENRISAVCSWWIPGLSTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLI4</accession>
    <name>A0A3B5LLI4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CTF/NF-I domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLI4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011802"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011938.1">
        <property type="protein sequence ID" value="ENSXCOP00000011802.1"/>
        <property type="gene ID" value="ENSXCOG00000008923.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182916"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000647">
        <property type="entry name" value="CTF/NFI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020604">
        <property type="entry name" value="CTF/NFI_DNA-bd-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003619">
        <property type="entry name" value="MAD_homology1_Dwarfin-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11492:SF3">
        <property type="entry name" value="NUCLEAR FACTOR 1 X-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11492">
        <property type="entry name" value="NUCLEAR FACTOR I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03165">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51080">
        <property type="entry name" value="CTF_NFI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CTF/NF-I" evidence="5">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="98" mass="11494" checksum="D347084F2FC5C431" modified="2018-12-05" version="1">MSKDEERAVKDELLGEKPEIKQKWASRLLAKLRKDIRPEFREDFVLTITGKKPPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLRRRQGHAPTPETES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNH0</accession>
    <name>A0A3B5LNH0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Serine/threonine-protein kinase TAO3</fullName>
            <ecNumber evidence="6">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Thousand and one amino acid protein 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22 29">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21 29">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane raft</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNH0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013638"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013805.1">
        <property type="protein sequence ID" value="ENSXCOP00000013638.1"/>
        <property type="gene ID" value="ENSXCOG00000010331.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155735"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051493">
        <property type="term" value="P:regulation of cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000029">
        <property type="entry name" value="Serine/threonine-protein kinase TAO2, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000877">
        <property type="entry name" value="TAO kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051234">
        <property type="entry name" value="TAO_STE20_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47167">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TAO1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47167:SF10">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE TAO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16 24" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="18" id="KW-0234">DNA repair</keyword>
    <keyword evidence="14" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="28"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="307"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="396"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="554"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="24">
        <location>
            <begin position="450"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="24">
        <location>
            <begin position="747"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="324"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="337"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00040009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00043013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="887" mass="103713" checksum="E17023836D8E01A1" modified="2018-12-05" version="1">MPSSTRKGVPKDPELADLFFKDDPEEVFCDLHEIGHGSFGAVYFARNSYSNEVVAIKKMSYNGKQTTEKWQDIIKEVKFLGQLRHPNTIEYKGCYLKDNTAWVNLFNFNFCIFSEVHKKPLQEMEIAAITHGALLGLAYLHSHNMIHRDVKAGNILLTELGQVKLADFGSASIASPANSFVGTPYWMAPEVILAMDEGQYEGKVDIWSLGITCIELAERKPPLFNMNAMSALYHIAQNDSPTLQSNECNSLTNSFSPTFVRLAQHEFVRRERSPRILIDLIQRTKDAVRELDNLQYRKMKKILYQEKHNGPLGESQEEEEDSETPSCKMNSLGSNHSIPSTSVSTGSQSSSVNSIQEVLDDGCSDMTMMHPQDYSSTLESSAHTKKDHQYNWDDGIHRDHRPEPMHASSDKSSQASNYKHRAGFATIKSASLVTKQIHEHEQESELREQMSGYKRMRRQHQKQLIALENKLKAEMDEHRLKLQKEVETQANNAYIELEKLAKRHVVQTEKEMKTALADEKKFQQQIVAQQKKELTTFLDNQKKQYKLCKEKIKEEMNEDHSTPKKEKQERLSKHKENMQHSQAEEEAHLLTSQRVFYDRNCRAFKRKVMIKRHDVEQEQIREELNKKKTQKEMEHAMLIRHDESTQELEQRQLKTLQKLRMDLIRLQHQTELENQIEYNNRRERELHRKHVLELRQQPKNLKVLELQIKKQFQDTCKVQTKQYKALRHHQMEVTPKAEHKTVLKALKDEQTRKLAILAEQYEQSINEMMASQALRLDEAQEAECQALRQQLQQEMELLNAYQSKIKMQTEAQHEREQQKLEQKVSLRRAHLEQKIEEELGSLQKERTDRIKHLLDRQEREIDSFDMESMRQGFGNLGTLDFPKDDYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQB8</accession>
    <name>A0A3B5LQB8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Diencephalon/mesencephalon homeobox 1b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQB8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013237"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013401.1">
        <property type="protein sequence ID" value="ENSXCOP00000013237.1"/>
        <property type="gene ID" value="ENSXCOG00000010038.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155505"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030901">
        <property type="term" value="P:midbrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061074">
        <property type="term" value="P:regulation of neural retina development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050767">
        <property type="term" value="P:regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060041">
        <property type="term" value="P:retina development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000125">
        <property type="entry name" value="diencephalon/mesencephalon homeobox protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052488">
        <property type="entry name" value="DMBX_homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46639">
        <property type="entry name" value="DIENCEPHALON/MESENCEPHALON HOMEOBOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46639:SF3">
        <property type="entry name" value="DIENCEPHALON_MESENCEPHALON HOMEOBOX PROTEIN 1-A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="64"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="11">
        <location>
            <begin position="366"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="66"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="123"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="155"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="252"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="42902" checksum="4636A1559A274383" modified="2018-12-05" version="1">MQHYGVNGYSLHAMNSLSAMYNLHQQAAQQAQHAPDYRPSVHALTLAERLADIILEARYGSQHRKQRRSRTAFTAQQLEALEKTFQKTHYPDVVMRERLAMCTNLPEARVQVWFKNRRAKFRKKQRSLQKEQLQKQKEASGAAEDSPEDSKTDLTNTPNTTTTTTVVPEVRSPPPPSSSSSCHSESERLAAARPQPGEMSVEVNVTSPEPSDSESATEDNAEREEEEISGESRVKLREEVQADGSIQAGRNSPLSSPALPTSSSVTSGLVQSNSYASSPLSLFRLQEQFRQHMAATNNLVHYPSFDMSAPSSLPYLGMNVNMPSPLGSLPCQSYYQTLSQAQQVWNSPLLQAAPGSLPGSLNSKTTSIENLRLRAKQHAASLGLDTLPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQV5</accession>
    <name>A0A3B5LQV5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin specific peptidase like 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQV5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013175"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013336.1">
        <property type="protein sequence ID" value="ENSXCOP00000013175.1"/>
        <property type="gene ID" value="ENSXCOG00000009984.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002316"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032183">
        <property type="term" value="F:SUMO binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030576">
        <property type="term" value="P:Cajal body organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028890">
        <property type="entry name" value="Peptidase_C98"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033505">
        <property type="entry name" value="USPL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15294">
        <property type="entry name" value="RETINOVIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15294:SF3">
        <property type="entry name" value="SUMO-SPECIFIC ISOPEPTIDASE USPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15499">
        <property type="entry name" value="Peptidase_C98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="2">
        <location>
            <begin position="137"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="649"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="800"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="507"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="540"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="610"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="685"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="901" mass="99030" checksum="C791E6B3CA93C01F" modified="2018-12-05" version="1">MVNGYHKESPDVKTADWDDKDVQDRDDPAPPACSSSVGSSSDVLLASNEAEPPSLSPHLVYPDAAKQNNVPASNTCSRSNSSQAIYPDNIKTPSPLCNEPTMKESLVADVFPCRDVNGFRSESRDSLDKFVSVPKQFLWRNSNNLCWLDSLLAVLVNCKSLRKLRPEHQPQRSSVWRLLRKHEGIYGVPRVPSHVLQNAHVDLQNLRMSVFKLLQPKLHCKLGKTMETPVFAMPLLLKLDSWVERLFQATFLWEFKCSACKAITKERVEKALPTFTNIVPDWTPLNAAHLAPCNACCKKNERRTMLLESVPPVFALHFVEGLPHNNIRNYAFSFKGKRYSVTAVIQYSQQLKHFITWIYNEDGSWLEYDDLKNACETHQHLQIPTQEIHVVFWEEEEETQPSFCSLSKVLLEPPSADIEAVPSLKDLSTEELPTQTPDQSFLTSHNDTDIVCALSGDCSTSADVNTSIGDSTLLDTFEGLTHDDLITLTLVEFQPDSVQSEMRPVKETQQAEDPSSPVITENVVSSLSDSSVPAAGGNLCHNTDAQSAPSSSPCDSELSDNLVSDPTFVPNAKKRRSRTPNLRKTVRRQKGKKDPPPEPSETPKPLVCPAQSNTAAVTEQTSTVSSINNSPPLLTQKDRWSYILSRHPVNQSHKNISHPPPTQNGVISEVKQSHPVHSTPKPVKKPPIPARVPRPPLSTEERTGLPPKAAEMYGGFGAKNPNPVIPLPSSLPPAISSNHPAPVTWTPSTSPRLTEISSFKTPGSSKVSSGLGDTETLRYKLLKKLKAKKKKLAKLNQLLGTGGGAHLRPDSTDLNSPSTVSSSTYDGSACSDLLSPATTASNLSPDSTGFLEMLVSGQDGANQLDCVVSGAGAVTPTNYRTTQHEDHNFLEEFEFFSDFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRI8</accession>
    <name>A0A3B5LRI8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SEFIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRI8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014673"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014859.1">
        <property type="protein sequence ID" value="ENSXCOP00000014673.1"/>
        <property type="gene ID" value="ENSXCOG00000011138.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111286"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030368">
        <property type="term" value="F:interleukin-17 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039465">
        <property type="entry name" value="IL-17_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013568">
        <property type="entry name" value="SEFIR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15583">
        <property type="entry name" value="INTERLEUKIN-17 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15583:SF12">
        <property type="entry name" value="INTERLEUKIN-17 RECEPTOR C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08357">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51534">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017406119" description="SEFIR domain-containing protein" evidence="9">
        <location>
            <begin position="21"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="26"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="SEFIR" evidence="10">
        <location>
            <begin position="196"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="451" mass="49626" checksum="B6DE27491793D404" modified="2018-12-05" version="1">MFNNSFACVIFLSVQPTGESETVIPSYLIAPCLCFLVTRILTRKNIHIHKMKGMIKTRMQHNMSVTMVESPLRIGETGLSWNVTSVCKVQAEVQLCKKDMAGGQCEEVTGSKQQLKTTGWTAAHRVHWVRKHPSICHTLQPQCPFETHRERWILSLLVGFILICLAILGVYLIQGVLKGYVWRWLKDDDVKGAVGGGHVMLLYPPDDDKRLPELLCHLGSSLQALGFTVSLDLWSQGELSVLGPVPWLHSRLDRLKRQGGKVVLVLTQTTWTRAEEWGACSCENNEESIKGAEGDSALSRHVDVFGASLSCILADYLQGRAGERFMLVQFESLPPAPPGGYRPLPRLFCGLHLYSLPSQSLGFLTELAGSKQIASASARRKRAGGLRMASRVFAKRLSGFTAGTNVLHLAGVSPGCVGREEEDSGETLLLEPNLITPPSSPDTVVGKMDWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRP4</accession>
    <name>A0A3B5LRP4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Neural cell adhesion molecule 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRP4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013475"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013641.1">
        <property type="protein sequence ID" value="ENSXCOP00000013475.1"/>
        <property type="gene ID" value="ENSXCOG00000010217.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155743"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070593">
        <property type="term" value="P:dendrite self-avoidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000086">
        <property type="entry name" value="Neural cell adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001932">
        <property type="entry name" value="Neural cell adhesion molecule 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000636">
        <property type="entry name" value="Neural cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009138">
        <property type="entry name" value="Neural_cell_adh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075">
        <property type="entry name" value="BASIGIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075:SF100">
        <property type="entry name" value="FASCICLIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01838">
        <property type="entry name" value="NCAMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="13"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="108"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="202"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="295"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="467"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="593" mass="66015" checksum="CC97EF3584D969CB" modified="2018-12-05" version="1">MILNSTRTLFFSLEVQITPTHGEISLGESKFFMCDVVGVAKHVDWYGPSGERIAPNRPDLTVTRNDESSSTLTFYEAKTDSAGTYKCVATNGDQQAEATVKVKIFQRITFTNAPSRQEFTEGDNANIVCDVTSSPPPTVFWKYKGAKIQMEKDVRFKVLSNNHLQIRGIKKTDEGMYMCEARLMARGEIDLRPISVVVNVLPTIRIWQSEVNATAEIAQPATLTCAVDGYPEPMVTWLRNGVELRDEEKYGFNEDGSEMTIMDVTKLDEGEYTCVAKNKAGESEKELSLRVFVKPKITYIVNRTASETEEQVTVTCEASGDPTPTISWSYGEQYSHSLSSEETTPDGNVVVRSDARVSSLTLKYVKYTDAGQYLCTARSAIGEDEQTAYLEVRCEYCIVLTGPLAFFMPAESHSATAQMNQVSMWLFVICQLVQPDSIFGQATKGQLNKRGPEQEEWLVLNFEFNGNPNPPKLEGSVEPKGNSLKVSWLKQDDGGSPILHYLIRYKPIQTSQWKPEIRMPSGSEYVVLRGLDWDTEYDVYVVAENLKGKSPPATMSFRTSAEPEAFPATQGSSSVTYTLVLLISPLVIALLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSR9</accession>
    <name>A0A3B5LSR9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Actinin alpha 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSR9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012431"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012582.1">
        <property type="protein sequence ID" value="ENSXCOP00000012431.1"/>
        <property type="gene ID" value="ENSXCOG00000009392.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159343"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000005">
        <property type="entry name" value="Actinin alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="324" mass="37136" checksum="6791C13611B3ADDF" modified="2018-12-05" version="1">MVDYHTANNQSAGGAQTYMEQENDWDRDLLLDPAWEKQQRKVSELDYYDAASVNTRCQKICEQWDILGSLTHRRKESLERTEKQLESIDELYLEYAKRAAPFNNWMEGAMEDLQDMFIVHNIEEIQGLISAHEQFKSTLPEANKEREAIQAIQAEVQRIAQSNGIKLSSANPYTTITPQSIDSKWEKAMAMVPQRDNALQHELNKQNSNDSLRATFAAQANSVGAYIQAKMEEIGRISIEMNGTLEDQLTNLKEYQKTILSYMPEINKLEAHHQHIQEALIFDNQYTSYTMEVCRKGCRTQSIAPVILRILIGPQKTLKACTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LV00</accession>
    <name>A0A3B5LV00_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dual specificity protein phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.16</ecNumber>
            <ecNumber evidence="7">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Dual specificity phosphatase able to dephosphorylate phosphotyrosine, phosphoserine and phosphothreonine residues, with a preference for phosphotyrosine as a substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="7"/>
    <dbReference type="EC" id="3.1.3.48" evidence="7"/>
    <dbReference type="RefSeq" id="XP_027863120.1">
        <property type="nucleotide sequence ID" value="XM_028007319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LV00"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015998.1">
        <property type="protein sequence ID" value="ENSXCOP00000015803.1"/>
        <property type="gene ID" value="ENSXCOG00000011936.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114138196"/>
    <dbReference type="KEGG" id="xco:114138196"/>
    <dbReference type="GeneTree" id="ENSGT00940000166490"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033549">
        <property type="term" value="F:MAP kinase phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14515">
        <property type="entry name" value="DUSP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020405">
        <property type="entry name" value="Atypical_DUSP_subfamA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682">
        <property type="entry name" value="AGAP008228-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682:SF12">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01909">
        <property type="entry name" value="ADSPHPHTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="9">
        <location>
            <begin position="28"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="10">
        <location>
            <begin position="96"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="6">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR620405-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="184" mass="20883" checksum="A1C524E3146CD733" modified="2018-12-05" version="1">MSRNGQRQNLVVIKELELILDSCTLELTPVDEVWPNLYIGNVAVAQNKNRLHKLSITHVLNAAHSKQGSIGDQSFYGNTCVYFGIPAEDSDHFDLSQYFKSATDFIHKGLKSKDGNVLVHCIMGVSRSATLVLAYLMLRHRFSLRDALRQVVKKRAIYPNRNFLTLLLKLDEELTQKRTLCPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LV92</accession>
    <name>A0A3B5LV92_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">procollagen-proline 4-dioxygenase</fullName>
            <ecNumber evidence="5">1.14.11.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the P4HA family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.2" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LV92"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015883"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016080.1">
        <property type="protein sequence ID" value="ENSXCOP00000015883.1"/>
        <property type="gene ID" value="ENSXCOG00000010262.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156635"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000006">
        <property type="entry name" value="Prolyl 4-hydroxylase subunit alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000001">
        <property type="entry name" value="Prolyl 4-hydroxylase subunit alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013547">
        <property type="entry name" value="P4H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059068">
        <property type="entry name" value="TPR_P4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF221">
        <property type="entry name" value="PROCOLLAGEN-PROLINE 4-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08336">
        <property type="entry name" value="P4Ha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23558">
        <property type="entry name" value="TPR_P4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="7 14" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="9" id="KW-0847">Vitamin C</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017432754" description="procollagen-proline 4-dioxygenase" evidence="16">
        <location>
            <begin position="25"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="14">
        <location>
            <begin position="215"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="17">
        <location>
            <begin position="426"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="268"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="549" mass="63356" checksum="06E8E978B001E517" modified="2018-12-05" version="1">PLSFRMELPCWCLLLLSCLQLLSAHNDFFTSIGQMTDLLYTEKDLVTSLKDYIRAEENKLERVKRWADKLDSLTATATQDPEGFLGHPVNAFKLMKRLNTEWGDLESLVLSDTTDGFISNLTIQRQYFPTEEDQTGAAKALLRLQDTYKLDANTISTGDLPGKSCYFSKYQMTVEDCYELGKIAYSDADYYHTELWMAQALKQLDEGEESTIDKVTVLDYLSYAIYQQGEIERALEYTLRLLKLDPEHQRAKGNVKYFEFQLEKQKKEETARTQEESGKRVTSEKNKKKKQNNSFSLIPERKKYEMLCRGEGLKMTPRRQSRLFCRYYNNNRHPRFVLAPVKQQDEWDRPYIVRYLDIISDAEIEKVKQLAKPRLRRATVHDPQTGKLTTAQYRVSKSAWLTGYEDPVIERINERIEGITGLEVDTAEELQVANYGVGGQYEPHFDFGRKDEPDAFKELGTGNRIATWLFYMSDVTAGGATVFPDVGAAVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWIHERGQEWRRPCSLNETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LW10</accession>
    <name>A0A3B5LW10_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">BRG1-associated factor 57</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LW10"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015060"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015249.1">
        <property type="protein sequence ID" value="ENSXCOP00000015060.1"/>
        <property type="gene ID" value="ENSXCOG00000011412.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003628"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016922">
        <property type="term" value="F:nuclear receptor binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0031492">
        <property type="term" value="F:nucleosomal DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21983">
        <property type="entry name" value="HMG-box_SMARCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000011">
        <property type="entry name" value="Putative SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46232">
        <property type="entry name" value="SMARCE1 REGULATOR OF CHROMATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46232:SF1">
        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY E MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="HMG box" evidence="15">
        <location>
            <begin position="64"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="12">
        <location>
            <begin position="64"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="39"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="151"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="326"/>
            <end position="412"/>
        </location>
    </feature>
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        <location>
            <begin position="222"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="151"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00067740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="412" mass="45956" checksum="9685D488354E8F21" modified="2018-12-05" version="1">TTERPSYAPPPPPAPTTQMPNTPGFVGYNPYSHLAYNNYRLGGSQSSNSRNSSGINIPKPPKPPDKPLMPYMRYSRKVWDQVKASNPDLKLWEIGKIIGGMWRDLTDEEKQDYLNDYEAEKIEYNESMKAYHNSPAYLAYVNAKSRAEAALEEESRQRQSRLDKGEPYMSIQPAEDPDDYDDGFSVKHTAAARFQRNHRLISEILSESVVPDVRSVVTTARMQVLKRQVQSLMVHQRKLEAELLQIEDRHQDKKRKFIEATESFNNELKRLCCMKVEVDMEKISAEIAAAEEAARKRMAEREKDAGDRGAREAPTCVLAEEEKHVCATHGKKSQQNSHSDSSSKEGEDAESNTADPPGSEEGASEDKESVPEGAMEEGTSDSNTGSETTSAQTEDATTSEPSEGPGKARAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LX18</accession>
    <name>A0A3B5LX18_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Myosin light chain 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">MYL7</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LX18"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012379"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012528.1">
        <property type="protein sequence ID" value="ENSXCOP00000012379.1"/>
        <property type="gene ID" value="ENSXCOG00000009341.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
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    </dbReference>
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        <property type="entry name" value="Putative myosin regulatory light chain sqh"/>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="MYOSIN REGULATORY LIGHT CHAIN 2"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="21"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="218" mass="24636" checksum="8352B0919E838FF5" modified="2018-12-05" version="1">MFLQRGNQKSCSNVFSMFEQSQIQEFKEAFGCIDQDRDGVIKKQDLKETYAQLGKLNIKDEELEEMLNEGKGPINFTVFLTLFGEKLNGTDPEDTILAAFKPFDPNGTGFVNKDDKTLLCTCYYYYFYSSIPLPAPPPAMALRATLGGAPHFGNHCTKASETWSWALIWMSHFSKLSYVVVSGRYNTQNLPTCLLPEIQQLGQLHKDKQAKGPMRNIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXU8</accession>
    <name>A0A3B5LXU8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Cytochrome c oxidase subunit 5A, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15 16">Cytochrome c oxidase polypeptide Va</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of a catalytic core of 3 subunits and several supernumerary subunits. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Mitochondrion inner membrane</location>
            <topology evidence="1 16">Peripheral membrane protein</topology>
            <orientation evidence="1 16">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the cytochrome c oxidase subunit 5A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXU8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016738"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016953.1">
        <property type="protein sequence ID" value="ENSXCOP00000016738.1"/>
        <property type="gene ID" value="ENSXCOG00000012611.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001424"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00923">
        <property type="entry name" value="Cyt_c_Oxidase_Va"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.40:FF:000002">
        <property type="entry name" value="cytochrome c oxidase subunit 5A, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.40">
        <property type="entry name" value="Cytochrome c oxidase, subunit Va/VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003204">
        <property type="entry name" value="Cyt_c_oxidase_su5A/6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036545">
        <property type="entry name" value="Cyt_c_oxidase_su5A/6_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14200">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14200:SF17">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02284">
        <property type="entry name" value="COX5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48479">
        <property type="entry name" value="Cytochrome c oxidase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 16" id="KW-0349">Heme</keyword>
    <keyword evidence="12 16" id="KW-0408">Iron</keyword>
    <keyword evidence="14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 16" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 16" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU368103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="141" mass="15939" checksum="83FFF97589E4E749" modified="2018-12-05" version="1">MFRALSRLSTSGFRSVARTRTGYSAPLASRCYSHGKVETEEEFDSRWVTYFSKPDIDAWELRKGMNTLIGYDLVPEPKILDSALRACRRLNDLASAIRILEAVKDKAGSHKEIYPYVIQELKPTLTELGISTPEELGIDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LY48</accession>
    <name>A0A3B5LY48_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LY48"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017050.1">
        <property type="protein sequence ID" value="ENSXCOP00000016833.1"/>
        <property type="gene ID" value="ENSXCOG00000012701.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183048"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF885">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="319" mass="36357" checksum="66C4B225CAEA8F38" modified="2018-12-05" version="1">MINSTQVLFFTLTAYFHSDVLNYLYFSVVLCLYALIVCSNVLLIVVICVNRTLHEPMYMFLCSLFVNELFGSLGLFPFLLVQILSDVHIISAPLCFLQIYCVYTYAHIEFCNLAIMSYDRYLAICRPLQYHALMTLSRIARLVALTWLFPLLESVAGILMTVSLQLCGNVIHKIYCHNYSIVKLACADTTASNVGGIVYMFTIILGIIILILYSYAEILKVCFSGSKQTRQKALSTCAPHLASLINFSFRCFFDLFQSRLDVRGVPEVLQVVLSLYFLTCQPLFNSLLYGLNLTTEIRQHILNTLTHWKRFVQTGPWCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LY56</accession>
    <name>A0A3B5LY56_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Caveolin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cell membrane</location>
            <topology evidence="1 6">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Caveola</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the caveolin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LY56"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012744"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012898.1">
        <property type="protein sequence ID" value="ENSXCOP00000012744.1"/>
        <property type="gene ID" value="ENSXCOG00000009630.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183006"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070836">
        <property type="term" value="P:caveola assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001612">
        <property type="entry name" value="Caveolin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018361">
        <property type="entry name" value="Caveolin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10844">
        <property type="entry name" value="CAVEOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10844:SF29">
        <property type="entry name" value="CAVEOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01146">
        <property type="entry name" value="Caveolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01210">
        <property type="entry name" value="CAVEOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="203" mass="23283" checksum="C2213F3C82952937" modified="2018-12-05" version="1">MDTLKKDEDSEEVEIDLEDSSDPEESEDGEEPQTLWRAQPSLDEEENIHTSTLVEISDTKPLITSRDARGVNNCLKVGFEDVIAEPPSVRSGDRVWIWSNALFEVSRVWIYRIVTAFLALPMSVYFFCLFLNINYIWMFSTELCNVCVFLFSYRMIRPCIHCVLIGNRWIQGFWDIMLQVVVVPFLTSAGKCCGSLGIHLAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYV4</accession>
    <name>A0A3B5LYV4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Post-GPI attachment to proteins GalNAc transferase 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027872702.1">
        <property type="nucleotide sequence ID" value="XM_028016901.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027872703.1">
        <property type="nucleotide sequence ID" value="XM_028016902.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027872704.1">
        <property type="nucleotide sequence ID" value="XM_028016903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYV4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016329.1">
        <property type="protein sequence ID" value="ENSXCOP00000016125.1"/>
        <property type="gene ID" value="ENSXCOG00000012185.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114144267"/>
    <dbReference type="KEGG" id="xco:114144267"/>
    <dbReference type="CTD" id="84302"/>
    <dbReference type="GeneTree" id="ENSGT00500000045018"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22190">
        <property type="entry name" value="PGAP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029675">
        <property type="entry name" value="PGAP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31410:SF1">
        <property type="entry name" value="POST-GPI ATTACHMENT TO PROTEINS FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31410">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="396" mass="45511" checksum="926353FD037B389E" modified="2018-12-05" version="1">MRQWKVFFSQHMRWSSALTQALTLSVITFCVVLPLCCHRLLYSYYFFRFTYLNSMSDEVLQKSLERGQDALHFWQSGSPMESARFSDVAQNPELLVTVVTARRNEGRDFHYLLQVMQQLSRIAGGCGGQRCAEVLLCDVESGPEENQDAKLLEGHFKVIRRSSQEQQANRESPNTFEREKRDYVFCLRQGWELVKPKNMILLEDDALPGEDFFRVVKDLLSRRFALHTLYIKLYHPERLQRYWNPEPYRILEWVGLGLVGATALLLSIPYCNPGSLSFTLSANHLVFFTLYFMVTAELIGRHYLLEVRRISPQLYAVSPATECCTPAMLFPGNASLRVADFLDGSFCYKGNAKDTVLYRKARTTPGERAHSVEPNLVTHIGAYSSVRPNPLRPKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZ81</accession>
    <name>A0A3B5LZ81_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphatase and actin regulator</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="5">Binds PPP1CA and actin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the phosphatase and actin regulator family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZ81"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016674.1">
        <property type="protein sequence ID" value="ENSXCOP00000016467.1"/>
        <property type="gene ID" value="ENSXCOG00000012429.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157628"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2130">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004018">
        <property type="entry name" value="RPEL_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12751:SF5">
        <property type="entry name" value="PHOSPHATASE AND ACTIN REGULATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12751">
        <property type="entry name" value="PHOSPHATASE AND ACTIN REGULATOR PHACTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02755">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00707">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51073">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RPEL" evidence="4">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU301113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="134" mass="16112" checksum="0ABD96D89DE91F37" modified="2025-10-08" version="2">MIVRGPVRLLFSIVHNVFLRLSQRPTTEELEQRNILKQINEAEEKEAKQEIKRRLTRKLSVRPTVAELIARRILRFNEYVEVTDAKDYDRRADKPWTRLTPADKAAIRKELNEFKSREMEVHEDSKQFTRFHRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZJ8</accession>
    <name>A0A3B5LZJ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tetratricopeptide repeat protein 39B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTC39 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZJ8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017298"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017519.1">
        <property type="protein sequence ID" value="ENSXCOP00000017298.1"/>
        <property type="gene ID" value="ENSXCOG00000013036.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182917"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019412">
        <property type="entry name" value="IML2/TPR_39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31859:SF4">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 39B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10300">
        <property type="entry name" value="Iml2-TPR_39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="552" mass="63537" checksum="13E498C5046BF9C1" modified="2018-12-05" version="1">MDLHTAIQETQCALNLVLNNRFSQALDLLQPWKHSMYHALGYSSILVMQATMTFDQKDIQTAMETIKQALQTCHRFRKRNSVVGSLSSLISKQSNLQEEEMHAELCYAECLLQKATLTFIQDENMISFIKGGIKIRTSYQIYKDCQNVLNISQDKAEQFNSFKQFEGGVKLGIGSFNLMLSLLPQKILRLLEFIGFSGNRSFGLSQLREGAASQSLRSIICVLTLLFYNTYVSLILGTGEGNLMEAEALLEPYQQKYPKGSIILFYSARIATLRGNFEKARSRFEECISSQQEWKQIHHLCYWELMWTHSFQQEWQQAYRYADLLCKESRWSKAIYMYQKAAILSMMTEDDVRKTGEDIVEVFRLQVEALKQRLAGKSIPMEKFAVRKSRRYKAAQPVPLVVPALEMMYVWNGFTIVGKRADYTESLLVTIEKAEQQLRNDTNPSEFHPDDSSLVQMLKGLCLKHLGRLLQAELCFTQVLSSESRIRYDHYLIPFSLYELGLLYKQQGDFVKAATYIEKAKTNYKDYSMESRLHFRIHAALSSLKGSPVSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZS1</accession>
    <name>A0A3B5LZS1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Somatostatin-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Somatostatin II</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Somatostatin inhibits the release of somatotropin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the somatostatin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZS1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017363"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017584.1">
        <property type="protein sequence ID" value="ENSXCOP00000017363.1"/>
        <property type="gene ID" value="ENSXCOG00000013084.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175991"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004250">
        <property type="entry name" value="Somatostatin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018142">
        <property type="entry name" value="Somatostatin/Cortistatin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10558">
        <property type="entry name" value="SOMATOSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10558:SF6">
        <property type="entry name" value="SOMATOSTATIN 1, TANDEM DUPLICATE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03002">
        <property type="entry name" value="Somatostatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001814">
        <property type="entry name" value="Somatostatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="7 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0372">Hormone</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Somatostatin/Cortistatin C-terminal" evidence="12">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="75"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="113"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001814-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="124" mass="13998" checksum="2A1F3D381F727011" modified="2018-12-05" version="1">IPDPRSSWILNLTFVSPLGVSSQADRDQDPYQNQNLDLDLELRHHRLLQRARSAGLLSQDWSKRAVEDLLAQMSRSEVEAQQDGPEAASRGTGSRLNLERSVETTARERKAGCKNFYWKGLTSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0R7</accession>
    <name>A0A3B5M0R7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase</fullName>
            <ecNumber evidence="2">2.7.8.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">4'-phosphopantetheinyl transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>apo-[ACP] + CoA = holo-[ACP] + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="EC" id="2.7.8.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:12069"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>apo-[ACP] + acetyl-CoA = acetyl-[ACP] + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46564"/>
            <dbReference type="Rhea" id="RHEA-COMP:9621"/>
            <dbReference type="Rhea" id="RHEA-COMP:9690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:78446"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:46565"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the P-Pant transferase superfamily. AcpS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.7" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0R7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016997"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017216.1">
        <property type="protein sequence ID" value="ENSXCOP00000016997.1"/>
        <property type="gene ID" value="ENSXCOG00000012819.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004663"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.20:FF:000003">
        <property type="entry name" value="L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="entry name" value="4'-phosphopantetheinyl transferase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055066">
        <property type="entry name" value="AASDHPPT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050559">
        <property type="entry name" value="P-Pant_transferase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12215:SF10">
        <property type="entry name" value="L-AMINOADIPATE-SEMIALDEHYDE DEHYDROGENASE-PHOSPHOPANTETHEINYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12215">
        <property type="entry name" value="PHOSPHOPANTETHEINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22624">
        <property type="entry name" value="AASDHPPT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="4'-phosphopantetheinyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="4'-phosphopantetheinyl transferase N-terminal" evidence="10">
        <location>
            <begin position="21"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="4'-phosphopantetheinyl transferase" evidence="9">
        <location>
            <begin position="120"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="309" mass="34561" checksum="3AAA9457186093BF" modified="2018-12-05" version="1">CTSPAERICMGSVRWGFRCGSWTPSRSEWLFAARCVQQEEKDRIGQFVFAKDAKSAMAGRLLLRKFVCEVMGIPWSQIRLNRSPRGKPYLAVTTGSDPPWSFNISHQGDYAVLAAQQGMQVGVDVMKTTLPGSNSIPEFFRIMTRQFTAHEWSTIQSAGSERQQLAAFYRHWTLKESFIKAIGTGLGFNLQRAEFHLSPELLTQGKVLRQTKMHLDEEPDDLWIFEESLLDVDHHVAVALGPADEAGSAKRKPLVLLVSSSQPLPPPAAFTLLSFGELIASASPLTEEDAAYWDSFRMKAEAPQRQKDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M180</accession>
    <name>A0A3B5M180_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein FAM33A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SKA2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M180"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017162"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017382.1">
        <property type="protein sequence ID" value="ENSXCOP00000017162.1"/>
        <property type="gene ID" value="ENSXCOG00000012939.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175043"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000940">
        <property type="term" value="C:outer kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026762">
        <property type="entry name" value="Ska2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042091">
        <property type="entry name" value="Ska2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32017">
        <property type="entry name" value="SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32017:SF3">
        <property type="entry name" value="SPINDLE AND KINETOCHORE-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16740">
        <property type="entry name" value="SKA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="12" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ska2 N-terminal" evidence="14">
        <location>
            <begin position="2"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="133" mass="14855" checksum="8A662CBF2980B7C2" modified="2018-12-05" version="1">MEQTVDKLESMFLKSEADLEYIERRLKLDFINGASESGSFAKENVTGILENLKSIKAKHSLLRCQVSQITDAQKGSMELIRNRLGTAMELIKHCQQTSHLEVESLAESEQGSAMFLDSTISSITAEVRFLTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3Y8</accession>
    <name>A0A3B5M3Y8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Splicing factor 3b, subunit 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RSE1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3Y8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018080"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018309.1">
        <property type="protein sequence ID" value="ENSXCOP00000018080.1"/>
        <property type="gene ID" value="ENSXCOG00000013628.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183151"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000027">
        <property type="entry name" value="Splicing factor 3B subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000031">
        <property type="entry name" value="Splicing factor 3b subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="8">
        <location>
            <begin position="14"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="9">
        <location>
            <begin position="445"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="7">
        <location>
            <begin position="864"/>
            <end position="942"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="976" mass="108397" checksum="CC30DA38C5C8B16B" modified="2018-12-05" version="1">MFLYNITLQRATGITHAIHGNFSGTKMQEIVVSRGKILELLRPDANTGKVHTLLTMEVFGIIRSLMAFRLTGGTKDYIVVGSDSGRIVILEYHPSKNQFEKVHQETFGKSGCRRIVPGQFLAVDPKGRAVMIGAIEKQKLVYILNRDAAARLTISSPLEAHKANTLVYHVVGVDVGFENPMFACLEMDYEEADNDPTGEAAANTQQTLTFYELDLGLNHVVRKYSEALEEHGNFLITVPGGSDGPSGVLICSENYITYKNFGDQPDIRCPIPRRRNDLDDPERGMIFVCSATHKTKSMFFFLAQTEQGDIFKVTLETDEEMVTEIRLKYFDTIPVATAMCVLKTGFLFVASEFGNHYLYQIAHLGDDDEEPEFSSAMPLEEGDTFFFQPRPLKNLVLVDEQENLSPIMSCQIADLANEDTPQLYVSCGRGPRSTLRVLRHGLEVSEMAVSELPGNPNAVWTVRRHVEDEFDAYIIVSFVNATLVLSIGETVEEVTDSGFLGTTPTLSCSLLGEDALVQVYPDGIRHIRADKRVNEWKTPGKKTIVRCAVNQRQVVIALTGGELVYFEMDPSGQLNEYTERKEMSADVVCMSLANVPPGEQRSRFLAVGLVDNTVRIISLDPSDCLQPLSMQALPAQPESLCIVEMGGVEKQDEIGDKGTIGFLYLNIGLQNGVLLRTVLDPVTGDLSDTRTRYLGSRPVKLFRVRMQGQEAVLAMSSRSWLSYSYQSRFHLTPLSYETLEYASGFASEQCPEGIVAISTNTLRILALEKLGAVFNQVAFPLQYTPRRFVIHPETNNLILIETDHNAYTETTKAQRKQQMAEEMVEAAGEDERELAAEMAAAFLNENLPEAIFGAPKAGAGQWASLVRLVNPIQGSTLDQVQLEQNEAAFSVAVCRFPNTGDDWYVLVGVARDMILNPRSVGGGFIYTYRLTAGGEKLEFVHKVGLITGNVVMKCFLLGKKEQHFFTVIFSFILDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4A8</accession>
    <name>A0A3B5M4A8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Frizzled class receptor 3a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor Fz/Smo family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4A8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016256"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016462.1">
        <property type="protein sequence ID" value="ENSXCOP00000016256.1"/>
        <property type="gene ID" value="ENSXCOG00000012283.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156491"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042813">
        <property type="term" value="F:Wnt receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035567">
        <property type="term" value="P:non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000006">
        <property type="entry name" value="Frizzled-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000539">
        <property type="entry name" value="Frizzled/Smoothened_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF22">
        <property type="entry name" value="FRIZZLED-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01534">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00489">
        <property type="entry name" value="FRIZZLED"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01330">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="321"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="361"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="403"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="12">
        <location>
            <begin position="56"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="13">
        <location>
            <begin position="232"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="562"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="562"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="597"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="620"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="636"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="61"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="69"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="660" mass="74582" checksum="5C13067B5BB1460F" modified="2018-12-05" version="1">MSSVKKKTPVSRGDGRTKTVINGCRQNVPSCYLSILLLLLLLFSPGSPHMEFIESHSEFSCEPIRLRMCQDLPYNTTFMPNLLNHYDQQTAALAMEPFHPMVNLVCSADLRMFLCALYAPVCTEYGRMSMPCRSICQRAKDECHKLMEIFGVAWPDDMECSRFPDCDEPYPRAEDLLTGPEPTDQSSMTVQRDYGFWCPRELKVDPDLGYSFMGRKDCSAPCPSMFFSQQELTFMRYFIGVVSIICLSATLFTFLTFLIDVTRFRYPERPIIFYAVCYVMVSLVFFLGFLLEDRVACNAVSPAQFKASTITQGSHNKPCTLFFMVLYFFTMAGSVWWVVLTITWFLAAVPKWGSEAIEKKALLFHAVAWGLPGALTVSLLALNKIEGDGISGVCFIGLYDIDALRWFVLAPLCFNVAVGISLLLVGIIALNRVRMEIPLEKENQAKLVKFMIRMGVFSVLYLAPLFTVIGCYIYEHTYRGIWETTWMEENCRRYHIPCPYMVKENKGIAQHSIRNCHLFHSFNLCEKDDGAHHNSKTMLITVSFKKAFLLCISLQGTSTHASSTHLAPSSVHSKASYHGSLRRNRDDRYDSLAYRGTPDRNLHGSMPRLDHPLSTHSSLHKIDSHSRHSSQRDTSEVSPTLITHGTTGSDGRPAEDGTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5H6</accession>
    <name>A0A3B5M5H6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Syntaxin-1A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Epimorphin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Syntaxin-2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Essential for epithelial morphogenesis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.</text>
    </comment>
    <comment type="function">
        <text evidence="20">Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis. Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, STX1A and VAMP2 which mediates the fusion of synaptic vesicles with the presynaptic plasma membrane. STX1A and SNAP25 are localized on the plasma membrane while VAMP2 resides in synaptic vesicles. The pairing of the three SNAREs from the N-terminal SNARE motifs to the C-terminal anchors leads to the formation of the SNARE complex, which brings membranes into close proximity and results in final fusion. Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm. Also plays an important role in the exocytosis of hormones such as insulin or glucagon-like peptide 1 (GLP-1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Interacts with SYT6 and SYT8; the interaction is Ca(2+)-dependent.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Synaptic vesicle membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Synapse</location>
            <location evidence="18">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 25">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M5H6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016676"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016891.1">
        <property type="protein sequence ID" value="ENSXCOP00000016676.1"/>
        <property type="gene ID" value="ENSXCOG00000012577.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234627"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006836">
        <property type="term" value="P:neurotransmitter transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15880">
        <property type="entry name" value="SNARE_syntaxin1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00179">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000005">
        <property type="entry name" value="Syntaxin 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.70:FF:000001">
        <property type="entry name" value="Syntaxin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF84">
        <property type="entry name" value="SYNTAXIN-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00804">
        <property type="entry name" value="Syntaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00503">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15 26" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="17" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="16 28" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0770">Synapse</keyword>
    <keyword evidence="9" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="10 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 28" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="29">
        <location>
            <begin position="197"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="26">
        <location>
            <begin position="74"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00040530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00046172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00060077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00066301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00072672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00082918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU003858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="293" mass="33408" checksum="2DCF3C574A0904F4" modified="2018-12-05" version="1">SGRRAQECGRAKVKETGDGFQGEMTTASWRRIHLEPGLSAEQVEEIRGFIETLAEKVEEVKRKHSAILASPNPDEKTKAELEDLMADIKKLANKIRSKLKSIQQTIEQEEGQNRSSADLRIRKTQHSTLSRKFVEVMSEYNTTQSDYRERCKGRIQRQLEITGRNTTNEELESMLESDNPAIFTSGIIMDNITQQAMNEIETRHNEIIKLENSIRELHDMFMDMAMLVESQGEMIDRIEYNVDHSVDYVERAVSDTKKAVKYQSKARRKKIMIIICCVVLGIVIASIVGGTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M632</accession>
    <name>A0A3B5M632_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Family with sequence similarity 171 member B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM171 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M632"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019903.1">
        <property type="protein sequence ID" value="ENSXCOP00000019658.1"/>
        <property type="gene ID" value="ENSXCOG00000014767.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183184"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd01671">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018890">
        <property type="entry name" value="FAM171"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049175">
        <property type="entry name" value="FAM171_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048530">
        <property type="entry name" value="FAM171_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31626:SF2">
        <property type="entry name" value="PROTEIN FAM171B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31626">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20771">
        <property type="entry name" value="FAM171A1-2-B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10577">
        <property type="entry name" value="FAM171A1-2-B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="FAM171 N-terminal" evidence="10">
        <location>
            <begin position="47"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="FAM171 C-terminal" evidence="11">
        <location>
            <begin position="427"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="439"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="533"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="537"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="677" mass="73961" checksum="2A43232EC95A675E" modified="2018-12-05" version="1">IKSSSAPPPAAGVLFLFALSQTNLFPSWPVGALHQEPLKTLSSSFILRVQVSDMLTGRPLSKAAVELFVNHTLRSSAFSGDGGDARLHVPFHAGLPVAVATSKQGFISALLLWETSRKPIFSSVTLSLLALTRGNIWLFDDSVLITSRTAVSQATVSFPQRLLNLTHGGNLTAIRSYLTVPRLTSDQGDSLNTLGIVSSKSGFVSVELSPVAAVSVRLFSGDVELNVSGPIQISLNVPESRGLQESDVVPAWFFSRTTGGWMRRGLGKMTLVDGKLMWTFTAPHLGYWIAAPVTSSFFGLQTLVDFILQHSFFLVVLVGGTLVVIVCLLFGLLCYCRKDQTTSTDDEESETSGQNQNQRNGFCVPAKSSDVIANPSAVVVSLDGNELDFIGATSEQTRTPDGLFFYNQPVAILPAPAFFHLEEHPDQSDWSRSATLPRATNGATAAEPQRKESFTQTLQTEEQLRTADGSEPASRTTFGLPESASVPGTLNKLAGNRHSVHAVMGLSKVALPQPPRAWFVSLEGKPAAEIRYAGSEQQRRRQTMDSRETSLDSGVDLSELNQTTGRRVTLERNATFIKNPTNKNTQHLWLSADSAHILNECGDILSVNEHKKITEKPKEEQMSLLLEMIIRKGPDTCEAFMDILRSNQGRYHQLQQFFSSSSDGQETSHQIYHLNFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6K7</accession>
    <name>A0A3B5M6K7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">KPNA2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the importin alpha family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6K7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019858"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020104.1">
        <property type="protein sequence ID" value="ENSXCOP00000019858.1"/>
        <property type="gene ID" value="ENSXCOG00000014908.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244891"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Importin subunit alpha"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032413">
        <property type="entry name" value="Arm_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Arm"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="220"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="496" mass="53846" checksum="68C28FD4F15226A7" modified="2018-12-05" version="1">MSADTDNGARLFQFKNKGKDVNVSSAYISVITKLTGNVIFNFSSVDTNPFWFLFPKPTQDWSLEDIIGGVSSVNTEHQLLATQAVRKLLSKEKQPPVDSVIAAGLIPRLVGFLAQFDFPHIQFEAAWALTNVASGTSEQTAAVVEHLSEQAVWALGNIAGDGPNGRDLVIKHGVLHPLLALVTTSDLSVLPPAYLLNVAWTLSNLCRHKNPAPPLAAVQQMLPALVKLLHHDNSEVLADTCWALSYLTNDSNERIEVVVKAGPVPRLVQLLACGELSIVTPALRTIGNIVTGTDEQTQCVLNAGALTMFPALLRHHKPSVQKEAAWTVSNITAGKSSQIQEVINAGLIPILVEVLQQGDYKTQNEAVWAVTNYTSGGTVDQVAYLVHCNILEPLLNLLTVKDSKAILVILEAVSNILQMAQSTGEVEKLCLMIEELGGLDKIETLQTHDNEAIYKSALKIIEKFLSEENADEPPAPDAAQDSYAFVIPDNKNTFQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M705</accession>
    <name>A0A3B5M705_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Forkhead box C1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M705"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020023"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020270.1">
        <property type="protein sequence ID" value="ENSXCOP00000020023.1"/>
        <property type="gene ID" value="ENSXCOG00000015028.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162303"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030325">
        <property type="term" value="P:adrenal gland development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001568">
        <property type="term" value="P:blood vessel development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061300">
        <property type="term" value="P:cerebellum vasculature development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061386">
        <property type="term" value="P:closure of optic fissure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061371">
        <property type="term" value="P:determination of heart left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071910">
        <property type="term" value="P:determination of liver left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035469">
        <property type="term" value="P:determination of pancreatic left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060059">
        <property type="term" value="P:embryonic retina morphogenesis in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048387">
        <property type="term" value="P:negative regulation of retinoic acid receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033339">
        <property type="term" value="P:pectoral fin development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072149">
        <property type="term" value="P:podocyte cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072015">
        <property type="term" value="P:podocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039021">
        <property type="term" value="P:pronephric glomerulus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030500">
        <property type="term" value="P:regulation of bone mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040036">
        <property type="term" value="P:regulation of fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008593">
        <property type="term" value="P:regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000583">
        <property type="term" value="P:regulation of platelet-derived growth factor receptor-alpha signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043114">
        <property type="term" value="P:regulation of vascular permeability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001756">
        <property type="term" value="P:somitogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035886">
        <property type="term" value="P:vascular associated smooth muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd20044">
        <property type="entry name" value="FH_FOXC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000016">
        <property type="entry name" value="Forkhead box protein I1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047391">
        <property type="entry name" value="FOXC1/C2-like_FH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF68">
        <property type="entry name" value="FORKHEAD BOX PROTEIN C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="74"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="74"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="174"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="174"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="264"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="277"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="452" mass="49956" checksum="44EFCA98C87A45BC" modified="2018-12-05" version="1">MQARYSVSSPNSLGVVPYISSDPSYYRAAAGGGYTGMAAPMNMYSHAAHDQYPASMARAYGPYTPQPQPKDMVKPPYSYIALITMAIQNSPDKKVTLNGIYQFIMERFPFYRDNKQGWQNSIRHNLSLNECFVKVPRDDKKPGKGSYWTLDPDSYNMFENGSFLRRRRRFKKKDALKEKEERLQKDVPPRQQGRDQDQPVQGSQPVRIQDIKTENGAVTPPQADSPSLNAVPKIESPDSSSSMSSGSPHSIPSSRSIAMDSSEHPQQQHHSQASTQGFSVDNIMTSLRGSPQGVTELPSSLTTSVYSSPCNQNSISTTSNATYHCNMSLYAGDRSSHLAPSTVDETLPDYSVTTTSSSLTHANLNSGQEGPHTHQGRLASWYLNQAGDLGHLGATYPAQQQNFHSVREMFESQRIGLNNSPVNGNNSCQMSFPPSQSIYRTSGAFVYDCSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAR8</accession>
    <name>A0A3B5MAR8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Sterol regulatory element-binding protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Sterol regulatory element-binding transcription factor 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 2), which is embedded in the endoplasmic reticulum membrane. Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">COPII-coated vesicle membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the SREBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAR8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021343"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021604.1">
        <property type="protein sequence ID" value="ENSXCOP00000021343.1"/>
        <property type="gene ID" value="ENSXCOG00000015973.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157339"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010886">
        <property type="term" value="P:positive regulation of cholesterol storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18922">
        <property type="entry name" value="bHLHzip_SREBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000016">
        <property type="entry name" value="Sterol regulatory element-binding transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46062">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46062:SF3">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="19" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="16" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="BHLH" evidence="24">
        <location>
            <begin position="67"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00038460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00039750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00042214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00045168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="311" mass="34034" checksum="7288CD3DB8ED4C42" modified="2018-12-05" version="1">LKPDGTQVLSTMQSPAGITTLTTPIQNTTLMGSNILTTVPVMMGGGDKLPIKQLQPGSAPCATAEGERRTTHNIIEKRYRSSINDKIVELRDLVMGSDAKMHKSGVLRKAIDYIKYLQQVNHKLRQENLSLKMANQKNRSILCAPEPVILSEDVDLKQEGVMMSPPASDSGSSSPHQFSPYCVDSEPGSPLLDHHQVKHVRAGLMKYLDVIRTGFIQFHCFFCCVSKVKSEPDSPSTVGVMDRSRLLLCALTFFCLSLNPLPSLLGSEDPGSHGLAAAHVPTRTLVWFPTHTQDFGEQQSLPRASRVCGIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAR9</accession>
    <name>A0A3B5MAR9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAR9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016804"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017021.1">
        <property type="protein sequence ID" value="ENSXCOP00000016804.1"/>
        <property type="gene ID" value="ENSXCOG00000012679.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158330"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000007">
        <property type="entry name" value="Leucine-rich repeat and calponin homology domain-containing protein 1 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF44">
        <property type="entry name" value="LEUCINE RICH REPEATS AND CALPONIN HOMOLOGY DOMAIN CONTAINING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="342"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="372" mass="40665" checksum="A4CFDD4AE00DBF2C" modified="2018-12-05" version="1">MAASVLLGSAESTGLNFTVGAGGNAILGNSNGLGQAGPASWNRSLDRALEEASVSGCLNLSGRKLKEFPRSAANHDLTDTVRADLSRNRLSELPLEVCLFVSLEYLNLYQNCLRSLPDGLVNLQALTHLNLSRNQLSILPAVVCSLPLKVLIASNNKLVSLPEELGQLRHLTELDVSCNEIRTLPSQVGQLEALRDLNIRRNHLVRLPSELAELPLVRLDFSCNKVTSIPVCYRGLTQLQAIVLDNNPLQSPPAQICIKGKVHIFKFLNLEASKTTPELPEFDRRPLTSWSDPTHTRTIMLLLQNKSLMTDCSCVPAVLTRRIQGDRMARWTRASTAWTVETSGGWATRSDRTAGADPAGSDPELRSVLQGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBL6</accession>
    <name>A0A3B5MBL6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">G protein-coupled receptor 182</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBL6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020992"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021250.1">
        <property type="protein sequence ID" value="ENSXCOP00000020992.1"/>
        <property type="gene ID" value="ENSXCOG00000015705.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154307"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902037">
        <property type="term" value="P:negative regulation of hematopoietic stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001350">
        <property type="entry name" value="G10D_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047143">
        <property type="entry name" value="GPER1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24226:SF0">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24226">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 182 AND ESTROGEN RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00643">
        <property type="entry name" value="G10DORPHANR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="283"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="55"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="332"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="356"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="368" mass="42385" checksum="0B51F8DB832775E8" modified="2018-12-05" version="1">SQEHDTMTFDHNHSLDFLNGTPWFIYECTIELDANYRRIALFLIYLFIFMVGLLENLLVVWVNWRRRHSASGVLFCIINVSLSDLIVIVIQPFFMMEVTLDKVWLWGSFLCKVTNLIYVVNFYSSSFFLALMTLERYLALTKPSLPTLLPVVGRQRWLICGGLWLLSLFLALLENVHVDILEWDEPGCYMLPEHNFTEWFVSVAFLCTIFQFLGPAAIIITCNVLIARAVRTAPDVEGRRDVWLVHVYSLVFVVCWLPYHLVMLLMIIDDLDPFMFSCNVVEVLYFSFIVVQGLSLFHCVANPFLYNFLSKSFRRNLINTVVSYIPKDQMAEQLEVGPKPNTPNGGKEDPGRQRKMSNASTSQSDVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCJ4</accession>
    <name>A0A3B5MCJ4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCJ4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018991"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019228.1">
        <property type="protein sequence ID" value="ENSXCOP00000018991.1"/>
        <property type="gene ID" value="ENSXCOG00000014277.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156138"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="177" mass="20596" checksum="3995D32821FC9832" modified="2018-12-05" version="1">MIEKIADFHPYIQQVLGRKSPFPLILLPQFGGYWIEGTNHELSEAGDPEQHRPPSPSSRTKLECNTTATLYRKHFLGKVSCMTCKLYLQYQCSFFSFDLNFIKNGEKHLDCCRKARLCICKLHPQCIEVIQKHTNNRYHFCHKNTNKTFLGLVFISMSEEKIPGKAQVWYFFVPSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEF1</accession>
    <name>A0A3B5MEF1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Myosin tail domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEF1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021930"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022197.1">
        <property type="protein sequence ID" value="ENSXCOP00000021930.1"/>
        <property type="gene ID" value="ENSXCOG00000016388.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163461"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000010">
        <property type="entry name" value="Myosin heavy chain, isoform G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349">
        <property type="entry name" value="CINGULIN-LIKE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349:SF6">
        <property type="entry name" value="MYOSIN-6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="5" id="KW-0518">Myosin</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin tail" evidence="9">
        <location>
            <begin position="1"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="99"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="267" mass="30634" checksum="79691AB823F1F184" modified="2018-12-05" version="1">MEKSDIRLRAALEQTDRVRKTCENEMLEAAERVGLLSTQNAALLNQKRKLETDLSLLTGEVDEAVQESRSAGEKAKKAVTDAALMAEELKKEQDSSSLLERMKRNMEATVKDLQVKLDEAEQAAQRGGRKQLHKLETRVRELQLELLEERKRSEEFQKSLRRSERRAKELSLQSEEDRKNLLRMQELIEKLQAKAKSYKRQAETAEEQVSNTSVRFRKIQQDLDGAEERADIAETAVNKLRLRTREHSGAVVLVGPARSALWFTSNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEL2</accession>
    <name>A0A3B5MEL2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rho-related BTB domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027889648.1">
        <property type="nucleotide sequence ID" value="XM_028033847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEL2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021995"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022262.1">
        <property type="protein sequence ID" value="ENSXCOP00000021995.1"/>
        <property type="gene ID" value="ENSXCOG00000016432.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114154600"/>
    <dbReference type="CTD" id="792631"/>
    <dbReference type="GeneTree" id="ENSGT00940000158918"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01873">
        <property type="entry name" value="RhoBTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000069">
        <property type="entry name" value="Rho related BTB domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000177">
        <property type="entry name" value="Rho-related BTB domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000014">
        <property type="entry name" value="Rho-related BTB domain-containing protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="262"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="470"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00074748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="692" mass="79368" checksum="27089F5D3DC3903B" modified="2018-12-05" version="1">MDYERPNVETIKCVVVGDNAVGKTRLICARACNATLTQYQLLATHVPTVWAIDQYRVCQEVLERSRDVVDDVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLYHVKTMWYPEIKHFCPRAPVILVGCQLDLRYADLEAVNRARRPLARPIKSNEILPPERGREVAKELGVPYYETSVVAQFGVKDVFDNAIRAALISRRHLQFWKSHLRNVQRPLLQAPFLPPKPPPPIITVPPPPSTTEEHPVGLLEDPHCADVILVLQERQKIFAHKIYLATSSSKFYDLFSMDTQNEETERPPRGPLSGRELLMRAASFDVCESSDDGDRANLRACTSDGTLKDSEGTRRGKLLSSWSRAFVSIQEELVDDPVTYTPRFMTVVHMDLSIQLGPFRAVLRYLYTGQLDENEKELMQIAHIAELLEVFDLRMMVANILNNEAFMNQEITKAFHVRRTNRVKECLAKGTFSDVVFKLDDGTIMAHKPLLISSCDWMAAMFGGPFVESCTKEVLLPNTTRSCMRAVLEYLYMGRFCSRSDLDAMELIVLANRLCLPHLVALTELYTVTVLTESAMMGTDIDGDVLIYLDMAQFHGAQQLTGWCLHHICTNYNSVCRKFPREMKAKSTENQDYFEKHRWPPVWYLKEDDHYQRARKEREKEDYLYQRRQCKRKWLFWNLPSSPNSNSPSGSSAII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEU2</accession>
    <name>A0A3B5MEU2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Coagulation factor VIIi</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEU2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022202"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022471.1">
        <property type="protein sequence ID" value="ENSXCOP00000022202.1"/>
        <property type="gene ID" value="ENSXCOG00000016581.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154474"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000120">
        <property type="entry name" value="Putative serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.740.10:FF:000001">
        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000404">
        <property type="entry name" value="Weary, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.740.10">
        <property type="entry name" value="Coagulation Factor IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017857">
        <property type="entry name" value="Coagulation_fac-like_Gla_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035972">
        <property type="entry name" value="GLA-like_dom_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000294">
        <property type="entry name" value="GLA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012224">
        <property type="entry name" value="Pept_S1A_FX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050442">
        <property type="entry name" value="Peptidase_S1_coag_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278">
        <property type="entry name" value="COAGULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278:SF26">
        <property type="entry name" value="COAGULATION FACTOR VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00594">
        <property type="entry name" value="Gla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001143">
        <property type="entry name" value="Factor_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00001">
        <property type="entry name" value="GLABLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00069">
        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57630">
        <property type="entry name" value="GLA-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00011">
        <property type="entry name" value="GLA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50998">
        <property type="entry name" value="GLA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 13" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017284418" evidence="14">
        <location>
            <begin position="22"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Gla" evidence="17">
        <location>
            <begin position="67"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="113"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="16">
        <location>
            <begin position="221"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="472" mass="52501" checksum="CF049BE12CAAE4CC" modified="2018-12-05" version="1">MLLRSFCLVSALLWAVHSAEGQTKHPLTQTYAFRNTPEWLVLRMDAPSAVFVEKHEAASVLRRWRRANSGFLEELKRGNLERECIEEICDYEEAREVFEDDQQTRDFWKTYEKRDPCLVNPCFNNGTCIYMGASYECQCPEGYEGRYCQTVFEDSLGCLYQNGHCEHFCDGSGARRKCFCADGYQLADNGKKCVPKVAFPCGRVASPATGPNQTMQDQVRLVGASYCNHGDCPWQVLIKLNGSSHCGGVLVRPDWVITAAHCVHGKNLQQLVVVAGENNLDLKGDTEQTFDVNVVILHENYNQATGDSDIALVRINASVFLSQYAVPVCLPTKDFAERELLPVRYHTVSGWGKRTTGGNAFASSGHPLSPVLRKMNVPIVQNSQCSQKAKFNFTDNMLCAGYLDGNQESCRGNDGSPLVTKYGSTSFLTGVVAWGRGCSHPGYYGVYTKVAMNSFLLIMNEQSEQTDSRVQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEZ1</accession>
    <name>A0A3B5MEZ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chemokine interleukin-8-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Ligand for cxcr3.2. Chemotactic for macrophages.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intercrine alpha (chemokine CxC) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEZ1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018194"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018425.1">
        <property type="protein sequence ID" value="ENSXCOP00000018194.1"/>
        <property type="gene ID" value="ENSXCOG00000013704.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175443"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042056">
        <property type="term" value="F:chemoattractant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00273">
        <property type="entry name" value="Chemokine_CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000004">
        <property type="entry name" value="C-X-C motif chemokine"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001089">
        <property type="entry name" value="Chemokine_CXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033899">
        <property type="entry name" value="CXC_Chemokine_domain"/>
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        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015:SF210">
        <property type="entry name" value="C-X-C MOTIF CHEMOKINE 9"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="INTERLEUKIN8"/>
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    <dbReference type="PRINTS" id="PR00437">
        <property type="entry name" value="SMALLCYTKCXC"/>
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        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="6">
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            <begin position="34"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        <source>
            <dbReference type="ARBA" id="ARBA00010665"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="106" mass="12126" checksum="ED60D34668A1FC69" modified="2018-12-05" version="1">MFVSMSAIFLCKDTDFLTLSSKTSGSVVGNQVVNLRCRCITKERKPIGRRIVALEVHPVSSHCAEIQIIATLKMEKRKVCLDPKAPWVQKVLKNRRFPSQRHRILH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MF32</accession>
    <name>A0A3B5MF32_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MF32"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022302"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022575.1">
        <property type="protein sequence ID" value="ENSXCOP00000022302.1"/>
        <property type="gene ID" value="ENSXCOG00000016664.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166263"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:lamellipodium"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048812">
        <property type="term" value="P:neuron projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045773">
        <property type="term" value="P:positive regulation of axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061003">
        <property type="term" value="P:positive regulation of dendritic spine morphogenesis"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098974">
        <property type="term" value="P:postsynaptic actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of actin filament polymerization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11281">
        <property type="entry name" value="ADF_drebrin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11960">
        <property type="entry name" value="SH3_Abp1_eu"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Drebrin-like protein isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035717">
        <property type="entry name" value="Drebrin-like_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829:SF9">
        <property type="entry name" value="ADF-H DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829">
        <property type="entry name" value="CORTACTIN AND DREBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
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        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="ADF-H" evidence="11">
        <location>
            <begin position="3"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="659"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="293"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="358"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="499"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="382"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="411"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="500"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="528"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="571"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="618"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011039"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="717" mass="81672" checksum="66843271A7BB5A6B" modified="2018-12-05" version="1">MQTVNLDTYSLSLLTAKEDILNPRSSTNWALFTYEGVTNKLKLADSGAGGVAELAEKFHISKPQYGLCKVGGVDGGALRIALISWVGQNVDDYRKNECASHIPAIKHFFKEAHAFISAEKPEDVTEDRVRAELSKTQTQAPTQWVRRSSRSADKEELVGTNYRKTNAAMEMRLINRDSFWARAEREEEARKDEEKRRATEERRRLERERILKERRDAEERDRKMNEKLQMIEEQRFEHCMELYIHVLFSYQEQQQREHEEDMRARLRRSESIEKAAEAAALVSQRSMNPREFFRQLSSSSSQSPTSPGSSRSGKPFRRYQRSLTDTAFIFSKAEESSASSPYTSPLVSPFSRAPHSPVFQAVSPPRSPDFYPVTSPQKPRAPMSPPTSPIRPAPPVSALPSIPQSTNQFQAAAEPEASEPTEPSAPPASPSLLDFLPPTEIDENISQLLSEAEQQPLNFEPMQFLTNRPDLDTAEFNTPSLGNTKLVSDTGFKVQAVLVEADEEEELQEEQEGDEAETQPEVDSVTAEPERIDTTEVDKEEVQEVKEDEKEEQNELDKEEEHEEEGKGREPEEEEKEEKYEEEDNKKELEEDEEEKQEGSRQETDPSSLLEPAAMPQVEEEAEEAEEENNYSGYSQTLAEPAEFSEEEEESVRTGSSSDRHMCVRALYDYQAEDESEISFEPGDIIRDVETVDKAWWRGWSKDGRQGLFPANYVETI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGI7</accession>
    <name>A0A3B5MGI7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
            <location evidence="1 11">Extracellular space</location>
            <location evidence="1 11">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGI7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020226"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020474.1">
        <property type="protein sequence ID" value="ENSXCOP00000020226.1"/>
        <property type="gene ID" value="ENSXCOG00000015165.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159618"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060536">
        <property type="term" value="P:cartilage morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003144">
        <property type="term" value="P:embryonic heart tube formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048702">
        <property type="term" value="P:embryonic neurocranium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001958">
        <property type="term" value="P:endochondral ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060218">
        <property type="term" value="P:hematopoietic stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060037">
        <property type="term" value="P:pharyngeal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000002">
        <property type="entry name" value="Protein Wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF75">
        <property type="entry name" value="PROTEIN WNT-9A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 13" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017292114" description="Protein Wnt" evidence="13">
        <location>
            <begin position="29"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="182"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="297" mass="33148" checksum="E43C31CEEF316C30" modified="2018-12-05" version="1">MLDGHLLLGWLSFTVIVHLLSLPGPTTAYFGKHLEICLFFKPNFKTKQRISFLYAISSAGLTHALAKACSAGRMERCTCDEAPDLENRKAWQWGGCGDNLKYANKFVKEFLGKRSNKDLRARVDTHNTNVGMKVIKAGVETTCKCHGVSGSCTVQTCWRQLLPFHEIGNRLKLRYETSVKVGSSTNEATGEGDISTGRNQQQQQQHSLPGLSDQIPRTMDLLHIEDSPSFCKPSKYSAGTAARKCYKDQNCDAICCGRGHNTQSREVTGPCQCQVRWCCYVECKQCTQREEVYTCKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJ12</accession>
    <name>A0A3B5MJ12_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BCL2 like 10</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Bcl-2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJ12"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024318"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024611.1">
        <property type="protein sequence ID" value="ENSXCOP00000024318.1"/>
        <property type="gene ID" value="ENSXCOG00000018164.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01130000278292"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051400">
        <property type="term" value="F:BH domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090504">
        <property type="term" value="P:epiboly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097192">
        <property type="term" value="P:extrinsic apoptotic signaling pathway in absence of ligand"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007369">
        <property type="term" value="P:gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008630">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051279">
        <property type="term" value="P:regulation of release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001756">
        <property type="term" value="P:somitogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06845">
        <property type="entry name" value="Bcl-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.437.10">
        <property type="entry name" value="Blc2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036834">
        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046371">
        <property type="entry name" value="Bcl-2_BH1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026298">
        <property type="entry name" value="Bcl-2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002475">
        <property type="entry name" value="Bcl2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256">
        <property type="entry name" value="BCL-2 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11256:SF61">
        <property type="entry name" value="BCL2-LIKE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00452">
        <property type="entry name" value="Bcl-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00337">
        <property type="entry name" value="BCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50062">
        <property type="entry name" value="BCL2_FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Bcl-2 Bcl-2 homology region 1-3" evidence="4">
        <location>
            <begin position="38"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="194" mass="21422" checksum="6EAB92027BBC8384" modified="2018-12-05" version="1">MSCGLWKETVVVAEDYIRLRCSSPHPAPPPPSEPAAAMRRLAQDVEAKHQARFHSLAQGFLKHCGSDLCSNLRKVMDEMVGDGHFNWGRVVSLFAFAGVLARQLREQTGKNPVPDSGKQQELQQEPVSCRALAETIADYLEKHKKDWLQENNGWEGFCSYARNAREASQDSSMKTALVAVAGVGIAGLTFLLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJ31</accession>
    <name>A0A3B5MJ31_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Atos homolog protein A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Transcription regulator that syncronizes transcriptional and translational programs to promote macrophage invasion of tissues.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATOS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJ31"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024338"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024631.1">
        <property type="protein sequence ID" value="ENSXCOP00000024338.1"/>
        <property type="gene ID" value="ENSXCOG00000018171.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157573"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033473">
        <property type="entry name" value="Atos-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025261">
        <property type="entry name" value="Atos-like_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051506">
        <property type="entry name" value="ATOS_Transcription_Regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13199:SF13">
        <property type="entry name" value="ATOS HOMOLOG PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13199">
        <property type="entry name" value="GH03947P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13889">
        <property type="entry name" value="Chromosome_seg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13915">
        <property type="entry name" value="DUF4210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01177">
        <property type="entry name" value="DUF4210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Atos-like conserved" evidence="7">
        <location>
            <begin position="699"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="257"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="409"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="475"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="263"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="496"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="517"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="897" mass="99679" checksum="098A601EF20EE062" modified="2018-12-05" version="1">PSPNRIHATEEFFEYDAEEFLVFLTLLITEGRTPEYSVKGRTEGLHCPPAQSGMPPLHKHECSDKLPQCRQARRTRSEVILLWRNNIPIMIEVMLLPDCCYGDECPPSDPISDPVIKQDALLLERWTLQAVPRQSGDRFIEEKTLLLAVRSYVFFSQLSAWLSASHGIVPRNILYRISAADEDLVWQFSQPPSEHIFPVPNVSQSVALQVRVQSLPRQPTYPTLACSIHTGLPPIYKNYTFHNGELPQVNIPQCQGSPLFYQPARSPTPSRSNSPSPLPSGKAGKWLYTALNGSSDPTPVDDYTCGTSNTDQSKAPIPEPLRSFKNLSMAESSRCPSPRPSGSETNPLIGSLLQERQEVIARIAQRLNFCDPTAPPLPPGLFATDSPHKAMWGGNHDDKAAGKTKELETRPYESVGRAKPAPFSTPVTETSRFIKRESSSPKPAACRKLRMAEDSLIAQAVQDITRLIQERLAVPPLSPRHSRSTTSNGCANGHVPSHEPQRDGTHNAASHSPVCTEKPRGGPRAERRSPRAQNGAQFTLEETSLNGQADTRVPCRLRASPQEKFFVSRNCASALFFPLLLQNIIYHFLHHSPLFIAVLQRPHSPSPLRPYNSWKKQNRHSLDATATKAFHPCTGLPLLSSPVPQRKNQTGYFDLDISLTGCKGLPWASGKRVCPKRDENTDESHQLFSASAPPASLSLLGNFEECVLNYRLEPLGTVEGFTAEVGASGSFCPSHLTLPVDVSFYSVSDDNAPSPYMGVINLESLGKRGYRVPASGTIQVTLFNPNKTVVKMFVVMYDLRAMPAGHQTFLRQRTFSVPVRRDANNQTNRKPLIPGQSRTLRYLIHLRFQSSKSGKLYLHRDIRLLFSRKSMEVDSGAAYELQSFTESPIDPPFSPRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJ40</accession>
    <name>A0A3B5MJ40_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJ40"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023913.1">
        <property type="protein sequence ID" value="ENSXCOP00000023630.1"/>
        <property type="gene ID" value="ENSXCOG00000017654.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="80" mass="9184" checksum="C42E4B71D562901B" modified="2018-12-05" version="1">QKEKKKGISKKKHLQRGIMTLKTWSSWDVRLRLNLSRSTYRLNSKPTTQKGQNNPAGGQKGPVSPPPFYSFFLSIIHIVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJK2</accession>
    <name>A0A3B5MJK2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJK2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019724"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019970.1">
        <property type="protein sequence ID" value="ENSXCOP00000019724.1"/>
        <property type="gene ID" value="ENSXCOG00000014819.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157354"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="428"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="507"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="553"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="629"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="555"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="645"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="678"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="776" mass="84007" checksum="8EC5ED3F074E3475" modified="2018-12-05" version="1">MFSTLRFFEGFCLAGITLSLYVLRIELCLPTWRFSMTMVASFVVLGGQLLMPGVAYLCHDWQVLQAVIICPLLLMLSYIWIFPESLRWLLATQQYCRSKWIMGHIEKKNKVNLERDADNILAELQNALQKKPKRTCIVKMAGTRNLWKNIVVLCVNSLTGYGIHHCFARSMVSPENEEKTIFRDFYAEYYTKAGIAVASCIALCPAVSLMGRRGGLLMFMIITALASLLQLGLLNFKLLHVSAVSSEQNESTLNRDFSVAFSIIGMFSSHAVSNLSIFFCAEITPTVIRGGGVGLVLASAGFGMLTAPIMDLHNQKGYFLHHIIFACCTLICIICILLLPETRNQSLPETIDDGENYTRQPLLLQRKLGEQHLLLHQLEPNRDYSRVQDTPLHETANVALSTMASTASSAIDLTALATGDLSVTNNLKEGPDLLKPQDLSGHSSPFPLSASAMTRGKDTIIQPNKECLLSSSPLHKAPCATDPLLADAEELLAVQESADLLKENIHLEVNDTTPSPKSKDGTGTAALHGSSPPVIPQTLPASLLICTTPVIEPRPSSTLESPNPLENDTDEIITTSKSPVSEDAPGILEDLLPTSDLDQPAQPSPSITQQFDIAPKVEKIFETACTPYIRNSEPPHLDPAPSNGKDLSSHSSTPPSLVAVSPTHTCAPPADSDPTPFADSSSVNTTARSPTVLPVTDIVNTLHLESSTNIVQDSVVSVDLNSVQSNSNVDVVPAEAAPISLMDSTVSSPIDSGVIAIMDRETSSTENNTDNSTALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLH3</accession>
    <name>A0A3B5MLH3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-rRNA-processing protein TSR2 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in 20S pre-rRNA processing.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TSR2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLH3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021986"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022253.1">
        <property type="protein sequence ID" value="ENSXCOP00000021986.1"/>
        <property type="gene ID" value="ENSXCOG00000016428.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175071"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019398">
        <property type="entry name" value="Pre-rRNA_process_TSR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21250">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10273">
        <property type="entry name" value="WGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="108" mass="12671" checksum="6DEB7D2AEDD3CFA5" modified="2018-12-05" version="1">MMAASTASRELFTEGVRAVLQTWPVLQIAVDNGFGGVYGQQKADWMVDVVQQYFHDNGTTTFQKQDNFYCLFYLCFIRTYWKINYTLFNNQRKILFSQLLALKQKSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MM04</accession>
    <name>A0A3B5MM04_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein Z, vitamin K-dependent plasma glycoprotein b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MM04"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022176"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022445.1">
        <property type="protein sequence ID" value="ENSXCOP00000022176.1"/>
        <property type="gene ID" value="ENSXCOG00000016568.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159516"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000006">
        <property type="entry name" value="Versican core protein-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.740.10:FF:000001">
        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.740.10">
        <property type="entry name" value="Coagulation Factor IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017857">
        <property type="entry name" value="Coagulation_fac-like_Gla_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035972">
        <property type="entry name" value="GLA-like_dom_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000294">
        <property type="entry name" value="GLA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050442">
        <property type="entry name" value="Peptidase_S1_coag_factors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278">
        <property type="entry name" value="COAGULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278:SF35">
        <property type="entry name" value="PROTEIN Z, VITAMIN K-DEPENDENT PLASMA GLYCOPROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00594">
        <property type="entry name" value="Gla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00001">
        <property type="entry name" value="GLABLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00069">
        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57630">
        <property type="entry name" value="GLA-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00011">
        <property type="entry name" value="GLA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50998">
        <property type="entry name" value="GLA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="2" id="KW-0301">Gamma-carboxyglutamic acid</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Gla" evidence="11">
        <location>
            <begin position="45"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="91"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="18895" checksum="1F6A58EE20FD29F0" modified="2018-12-05" version="1">AVSVMAVHIMSASLLAVCLLGCFLQVFRFRVAPGAHAVFLRSKRANQFLLEEILQGNLERECYEELCNYEEAREVFEDDERTKSFWTIYYDGNQCLPNPCLNGGNCTDKVGGFFCACSPPNYGHICELGPVEVAGQELLKPQYKAPGKAAATAGGRRWFSNRFIQQRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MM15</accession>
    <name>A0A3B5MM15_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pleurocidin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MM15"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025756.1">
        <property type="protein sequence ID" value="ENSXCOP00000025449.1"/>
        <property type="gene ID" value="ENSXCOG00000019013.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012515">
        <property type="entry name" value="Antimicrobial12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08107">
        <property type="entry name" value="Antimicrobial12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0044">Antibiotic</keyword>
    <keyword evidence="4" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017286530" evidence="6">
        <location>
            <begin position="23"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="57" mass="6520" checksum="A7FA524692D59864" modified="2018-12-05" version="1">MKCSAAFLVLSWVVLLAQPGQGFFGLIIRLHAKSSLQKHTKYFFHLDFTANVFIHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MMA2</accession>
    <name>A0A3B5MMA2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Citramalyl-CoA lyase, mitochondrial</fullName>
            <ecNumber evidence="4">2.3.3.9</ecNumber>
            <ecNumber evidence="18">3.1.2.30</ecNumber>
            <ecNumber evidence="19">4.1.3.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">(3S)-malyl-CoA thioesterase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Beta-methylmalate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Malate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">CLYBL</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Mitochondrial citramalyl-CoA lyase indirectly involved in the vitamin B12 metabolism. Converts citramalyl-CoA into acetyl-CoA and pyruvate in the C5-dicarboxylate catabolism pathway. The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, a vitamin B12-poisoning metabolite. Also acts as a malate synthase in vitro, converting glyoxylate and acetyl-CoA to malate. Also displays malyl-CoA thioesterase activity. Also acts as a beta-methylmalate synthase in vitro, by mediating conversion of glyoxylate and propionyl-CoA to beta-methylmalate. Also has very weak citramalate synthase activity in vitro.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(3S)-citramalyl-CoA = pyruvate + acetyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:22612"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58668"/>
            <dbReference type="EC" id="4.1.3.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>glyoxylate + acetyl-CoA + H2O = (S)-malate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>propanoyl-CoA + glyoxylate + H2O = 3-methylmalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47628"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
            <dbReference type="ChEBI" id="CHEBI:87810"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit-like subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.9" evidence="4"/>
    <dbReference type="EC" id="3.1.2.30" evidence="18"/>
    <dbReference type="EC" id="4.1.3.25" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MMA2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024913"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025217.1">
        <property type="protein sequence ID" value="ENSXCOP00000024913.1"/>
        <property type="gene ID" value="ENSXCOG00000018601.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017163"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047777">
        <property type="term" value="F:(S)-citramalyl-CoA lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004474">
        <property type="term" value="F:malate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106064">
        <property type="term" value="P:regulation of cobalamin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000014">
        <property type="entry name" value="Citrate lyase subunit beta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005000">
        <property type="entry name" value="Aldolase/citrate-lyase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040186">
        <property type="entry name" value="Citramalyl-CoA_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011206">
        <property type="entry name" value="Citrate_lyase_beta/mcl1/mcl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11105:SF0">
        <property type="entry name" value="CITRAMALYL-COA LYASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11105">
        <property type="entry name" value="CITRATE LYASE SUBUNIT BETA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03328">
        <property type="entry name" value="HpcH_HpaI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015582">
        <property type="entry name" value="Cit_lyase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 25" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="HpcH/HpaI aldolase/citrate lyase" evidence="26">
        <location>
            <begin position="51"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00055540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00066460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00066840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00072098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00076231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00076788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00083020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR015582-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR015582-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF03328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="345" mass="38234" checksum="0E77BC18C7E79230" modified="2018-12-05" version="1">MSLCQCALTFVYICADRKNGWLPPAVWQLYTQACCHHHQSPGSSLRYIPRRAVLYCPGNDERKLKKLALLDVDCAVLDCEDGVALTKKAEARETIAQILGELDLGRTEKCVRINSVSSGLAEADLQVVLQAKVLPPAIMLPKVEDIQEAKWFVDRFQHNLKGRTLTEPVRLVTFVETAAGLLNFKAVCEEIRQLAPRAGLHHDAVVFGSDDFCASIGATRTKDARELLYARQKVVVTTKAFGLQAIDLVYIDYKDVEGLRLQAKEGALMGFTGKQVIHPGQIQAVQEEFSPSQDRVQWAEELITAFDQHQKEGKGAFTFRGSMIDLPSLKQAQNILTLAAAVSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNG9</accession>
    <name>A0A3B5MNG9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Tyrosine-protein kinase receptor</fullName>
            <ecNumber evidence="22">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 22">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="22"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025086"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025391.1">
        <property type="protein sequence ID" value="ENSXCOP00000025086.1"/>
        <property type="gene ID" value="ENSXCOG00000018729.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155404"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005899">
        <property type="term" value="C:insulin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005009">
        <property type="term" value="F:insulin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043560">
        <property type="term" value="F:insulin receptor substrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043548">
        <property type="term" value="F:phosphatidylinositol 3-kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042593">
        <property type="term" value="P:glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05061">
        <property type="entry name" value="PTKc_InsR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000050">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000005">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000087">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000108">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000026">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.20.20:FF:000001">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.20.20:FF:000002">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.20.20">
        <property type="entry name" value="Receptor L-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006211">
        <property type="entry name" value="Furin-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000494">
        <property type="entry name" value="Rcpt_L-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036941">
        <property type="entry name" value="Rcpt_L-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016246">
        <property type="entry name" value="Tyr_kinase_insulin-like_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002011">
        <property type="entry name" value="Tyr_kinase_rcpt_2_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF535">
        <property type="entry name" value="INSULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00757">
        <property type="entry name" value="Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01030">
        <property type="entry name" value="Recep_L_domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000620">
        <property type="entry name" value="Insulin_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00239">
        <property type="entry name" value="RECEPTOR_TYR_KIN_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 20 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 20 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 22" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16 22" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="594"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="25">
        <location>
            <begin position="809"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="974"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="731"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1276"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="731"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="19">
        <location>
            <position position="1110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="984"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20 21">
        <location>
            <position position="1008"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="1055"/>
            <end position="1061"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="1114"/>
            <end position="1115"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="1128"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000620-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000620-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU000312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1334" mass="151202" checksum="B8E52DC61EFBC672" modified="2018-12-05" version="1">MFLHFLVSSVCSSKDIRNNATNLRKLENCTVIEGHLKILLMFGTKPKDFQGLSFPKLRVVTDYLLLFRVYGLESLSSLFPNLTVIRGNNLFFNYALVIFEMLQLREIGLHSLMNITRGAVRIEKNSDLCYLSTLDWSKILDSVEDNYIVANKNDRECGDVCPGASIGKTTCPSTTINGHFSERCWTQSHCQRMCPINCTHRACTKDDDCCHYQCLGGCLRPNSPRHCVACRGLLHEDSCVERCPENYFTYKGWRCVSLAFCQDLHNRCKQEKSSDCYEYVIHNGACIPECPSGYSIGNSSTLNCTPCAGPCPKVCMGLKTVDSVTAAQALRGCTVLNGSLVINLRGGNNIAAELEASLGQLEEITGYLTVRRSYALVSLSFFRKLRLIRGEEQEIGNYSFYALDNQNLRQLWDWSKHKLTILQGRMFFHYNSKLCMTEIRKMEEVTGTKERRVKNEIASKTNGDQASCENRVLKFTQIKTMSDKIMIKWEPFWPPDFRDLLGFMVLYKEAPFQNVTEFDGQDACGSNSWVIADVDPPRRDKEQVEPGHLILPLKPWTQYAIMVKTQLSASDEHQVHGAKSDIIYVRTNATKPSVPLDPMSSSNSSSQIILKWKPPNDPNGNITHYLVFYQRQPEASELYKFDYCQKGMKLPSRVPTQVDSDEEQKWNQTEDQGQGKKCCACPKTDKELKKEKEDSEYRKTFENYLHNEVFEVKRPRQRRSVMGIANRTQPFLTTAASPPGSTGVPNDEDENKEYPKTLVVVYSKESTVISNLRHFTSYQIEVHACNDPTDQGRCSMAAYVSARTMPEDKADNILGDITHEVTENTVHLHWKEPAAPNGIIILYEVNYKRVGDAEELPHCVSRNMYKASRGCKLKVMHPGNYSVRIRATSLAGNGSWTEPSYFYVQDPSNAQLLHPFQSFILCIFITLSSQGVCSLPFSQTQGPSGPIYASSNPEYLSANDVYEEDEWEVPREKIAILRELGQGSFGMVYEGIAKDIVKGEGETHVAVKTVNESASLRERIEFLNEASVMKAFTCHHVVRLMGVVSKGQPTLVVMELMTHGDLKSYLRSLRPDAENNPGRPPPTLKEMIQMAAEIADGMAYLNAKKFVHRDLAARNCMVAHDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMAPESLKDGVFTAHSDCWSFGVVLWEISTLAEQPYQGLSNEQVLKFVMDGGFLDRPDNCADRLHNLMQMCWQYNPKMRPTFQEIIEMLKDDLHPSYREVSFFYSEDNKLPESEDFDLDMENMESIPLDPSSYSQREQCSDRDEASSMGLRGSYEEHHIPFTHMNGGKTNGRILALPRSSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPD1</accession>
    <name>A0A3B5MPD1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MPD1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025391"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025698.1">
        <property type="protein sequence ID" value="ENSXCOP00000025391.1"/>
        <property type="gene ID" value="ENSXCOG00000018965.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155252"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022613">
        <property type="entry name" value="CH_CAMSAP_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45818:SF5">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR VAV3 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45818">
        <property type="entry name" value="PROTEIN VAV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11971">
        <property type="entry name" value="CAMSAP_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="1">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="152" mass="17533" checksum="0C976E6484F9E496" modified="2018-12-05" version="1">MEYWRQCAMWLIGCNVLPANHRVTADTAQVFDLAQTLRDGVLLCQLLNNLKPHTINLKEINLRPQMSQFLCLKNIRTFLSSCCDVFGMKKSDLFESFDLFDVRDFGKVSLPPILSVYYERVVVTSCSLRILGIWSVTHQGSMLMESDCKHYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQL2</accession>
    <name>A0A3B5MQL2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Toll-like receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQL2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025806"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026116.1">
        <property type="protein sequence ID" value="ENSXCOP00000025806.1"/>
        <property type="gene ID" value="ENSXCOG00000019271.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163999"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002224">
        <property type="term" value="P:toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000001">
        <property type="entry name" value="Toll-like receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF522">
        <property type="entry name" value="LOW QUALITY PROTEIN: TOLL-LIKE RECEPTOR 13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="13" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="TIR" evidence="14">
        <location>
            <begin position="790"/>
            <end position="931"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="945" mass="108280" checksum="51BACA4C93CAE519" modified="2018-12-05" version="1">LTVLGTGALLFLLLFCLFLHINPCLTYSLKNCSILYEDNPSAGVSVDCANRDLVSIPDDIPKEAVSIELRGNNIQTIKDDFRGMSRLRYLGLRKSGITSVDDGCFNDLVSLETLMMRNNYLFALTANMFQGLSNLTTLDLSHSGICFIHGSTFRFLISLKVLNLDYNTLKNTADIQPIFQLPQIQTLSLRCNKLSSFESKNFLLNKSSSLQQLFISGVSLRKFSITTPIFPYLQVVELSTCLPVTKLKWDISDKTLLENITQFYVDENALSFKGMQNVMQNLISLRHFKVNMLVSWIKRGLLSAVCKIQSLRRLDFFYNNMNNKTLKLAPCSQLAELYLQETNLNELTNGSIQSMKMLQVLNMSDNKLTRVSNDIRSLAFLKILHLDKNFISKLDCEDFENTTRLMELNLNTNQIPSLERCVLEHLTDLKHLDLGYNKLRAFGDTFKVALHKLEFLDMSENDIDILSSNTLGGLQSLKHLKMASDDIMLMENETFPELRNLVDLNVSFPNGYKPELQGLQRLENLIVYFGKGFTFKNLYQNKHGGLSDLKLLKSLTVIGCTDLTFHLKNLKEMLQAMGHLEAFEAVDVFEEAPDVDTFQFNPHLSSLTLTNTNMSHLKPELFRPIPNLQRLDLTNSMLRSMDFLLQANLSALKYLKLTDNKIIMINEAVWEALPSLTYLDLSYNLFTCECSNAGFIQWVKTNKQTQIVDAHRYECSFPVSKEGTLLLDLHTQSCFNDASFLCFISTTCLVVLVLFTSLIYHFLRQHLVYGFYLFLAFLYDSKRKKKNDGHQFDAFVSYNVRDEGWVYREMLPVLEGQQGWRLCLHHRDFQPGKPIIENITDAIYGSRKTICVISRSYLQSEWCSNEFQMASFRLFDEKKDVLILLFLEEIPAHHLSPFYRMRKLVKKRTYLSWPQAAQHPGVFWKNVQRALQAGGAVTEPVPAGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSS7</accession>
    <name>A0A3B5MSS7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Transmembrane protein 237b</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the transition zone in primary cilia. Required for ciliogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMEM237 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSS7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027454"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027787.1">
        <property type="protein sequence ID" value="ENSXCOP00000027454.1"/>
        <property type="gene ID" value="ENSXCOG00000020498.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005159"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060027">
        <property type="term" value="P:convergent extension involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029409">
        <property type="entry name" value="TMEM237"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28388">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28388:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15383">
        <property type="entry name" value="TMEM237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="46"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="415" mass="46382" checksum="DAD7C5DC430A7F30" modified="2018-12-05" version="1">MRIIRKLFFLSLFHTSQDTAGGFNFFFRSHIVDLFLFLNSNGKPLTDFPAPKRKKKKPRKDANGEGDEMEMGGLGSRRASEVGDHLTLGATTDAPTQRKRKKKKAMSTDLEDNQADLVNGDTGDQTTDGEEVVKKTKKKKSKVAESPLPDEFCVEEDDIINDSCSPIPQQALFSAPQGQSQPVGKLFVERTKRFQAAERSDWRTTGEQMDGAAELQHMPPLWTTRDVSVRVHEGFRVFGLFCHGFLAGYAVWNVVVVYMLAGQHLTALRNLLEQYHGLAYPSQSLLYMLLAICTVASFDRVNLAKGPVALRQIITLDPVALASFLYFSALILSLSQQMTSDRINLYASSNTTIWPQGSEHQILHPWVTINLVVALLVGLAWIFIATRPETDYTEGYLRAMEVEPARPEDKPEITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MT86</accession>
    <name>A0A3B5MT86_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pumilio homolog 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic granule</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MT86"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026978"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027311.1">
        <property type="protein sequence ID" value="ENSXCOP00000026978.1"/>
        <property type="gene ID" value="ENSXCOG00000020151.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158079"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035196">
        <property type="term" value="P:miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043488">
        <property type="term" value="P:regulation of mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF1">
        <property type="entry name" value="PUMILIO HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="PUM-HD" evidence="9">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="71" mass="8276" checksum="47C9671D54B4B16C" modified="2018-12-05" version="1">MKDQYANYVVQKMIDVAEPTQRKIVMHKIRPHIQTLRKYTYGKHILAKLEKYYMKNGVDLGPICGPPNGIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MU57</accession>
    <name>A0A3B5MU57_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">YY1 associated factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">YAF2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MU57"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027939"/>
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        <property type="protein sequence ID" value="ENSXCOP00000027939.1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:transcription coregulator activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="YY1 associated factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RYBP/YAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033774">
        <property type="entry name" value="YAF2_RYBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12920">
        <property type="entry name" value="RYBP AND YAF2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12920:SF2">
        <property type="entry name" value="YY1-ASSOCIATED FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17219">
        <property type="entry name" value="YAF2_RYBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="11">
        <location>
            <begin position="19"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="64"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="120"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="148"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="164" mass="18070" checksum="4870731DC17F494D" modified="2018-12-05" version="1">MGDKKSPTRPKRQSKPFTDDGYWDCSVCTFKNSAEAFKCLMCDVRKGTSTRKPRPVAQLVAQQVNQQFASDKDPTLKKKNQKRMRPRLKNVDRSSAQHLEVTVGDLTVIITDFKEKVKSTSSSVSGASADQHSQSGSSSDNTERGVSRCSSPRGETSSINGETH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUA8</accession>
    <name>A0A3B5MUA8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
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        </lineage>
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    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:coenzyme A diphosphatase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
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    </dbReference>
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        <property type="entry name" value="CoAse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992">
        <property type="entry name" value="NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992:SF24">
        <property type="entry name" value="PEROXISOMAL COENZYME A DIPHOSPHATASE NUDT7"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="199" mass="22122" checksum="95A8CDAAFAC0630F" modified="2018-12-05" version="1">MNLKEETVATLKRFDIGDKFSYLPVLPKASVLIPLFVRDLLSSRDPSDRDDVDTALREAQEEIGLPPDQVEVVCTLFPVFSKRGLLVSPVVGFIKETFTPTPNPSEVSAVFAVPLDFFTREKDHCITHGVAGMTGPLHSFYFRDPASGSQYHIFGLTAMLATLVAVLALKKKPQFDVGFDLVDPLAFFKKILHKRLSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVC0</accession>
    <name>A0A3B5MVC0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Trimeric autotransporter adhesin YadA-like head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVC0"/>
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        <property type="protein sequence ID" value="ENSXCOP00000023479.1"/>
        <property type="gene ID" value="ENSXCOG00000017536.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000288905"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="Serralysin-like metalloprotease, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011049">
        <property type="entry name" value="Serralysin-like_metalloprot_C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101967">
        <property type="entry name" value="Adhesin YadA, collagen-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="8590" checksum="A50B8E6E73773664" modified="2018-12-05" version="1">MGNTSITEGKTALAVGNTSIARGKTTVSLGKSSIFRGVTTTSMGDSTIQRQKTTVALGRASFSRGTTTTSFRKALTSKRRTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MX67</accession>
    <name>A0A3B5MX67_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAD synthase</fullName>
            <ecNumber evidence="3">2.7.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">FAD pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">FMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>FMN + ATP + H(+) = FAD + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the MoaB/Mog family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.2" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MX67"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026172"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026490.1">
        <property type="protein sequence ID" value="ENSXCOP00000026172.1"/>
        <property type="gene ID" value="ENSXCOG00000019555.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007266"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00885">
        <property type="entry name" value="cinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23948">
        <property type="entry name" value="FAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056596">
        <property type="entry name" value="FLAD1_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293:SF9">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293">
        <property type="entry name" value="FAD SYNTHETASE-RELATED FMN ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24102">
        <property type="entry name" value="FLAD1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0288">FMN</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="MoaB/Mog" evidence="16">
        <location>
            <begin position="22"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="503" mass="55914" checksum="0AEB4052547ECAB7" modified="2018-12-05" version="1">MSTNSQGASSSSAVSADAPTAAILIIGDEILKGHTVDTNSTFLTRALRRLGVSVQRITVIPDMHEVIAKEVVVLSSQYTHLITSGGVGPTHDDITFESIAAAFQEEVYPHPDLTQLVEEYFGVTDTDSPAMKLAMVPRSAKLNYGIDPQTGKRQRYPVVSVRNVYVFPGTPSFMELAFTGLEHLFASSGTTFHSREVFVDADETEIAPVLTKLQASWGRRVALGSYPEWLSNYCKVRLVLDSGSQEEVDKARAQLVDQLPKGSVVSLIKDPVTIAPAEVYRLANSGTALGEKVKCALKTIETALDQYATEQVCVGFNGGKDCTALLHLYYSTLKRRYPDGKDKVKALYIRNVSPFPEMERFLQGTTKKYDLELISVEGSIRQALNELHDKRPELKAVLMGTRRSDPYSHTLTPMCPTDPGWPEYMRVNPLLVSHVHVNSSCLISIHCTFVFLFTYFFVQYTSLGSMDNSYRNKALQVVDERGVTRYKPAYLLENEEEERNSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MY63</accession>
    <name>A0A3B5MY63_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">SAP30 like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAP30 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027887821.1">
        <property type="nucleotide sequence ID" value="XM_028032020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MY63"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025150"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025455.1">
        <property type="protein sequence ID" value="ENSXCOP00000025150.1"/>
        <property type="gene ID" value="ENSXCOG00000018785.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114153462"/>
    <dbReference type="KEGG" id="xco:114153462"/>
    <dbReference type="CTD" id="79685"/>
    <dbReference type="GeneTree" id="ENSGT00390000006633"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1800.30:FF:000001">
        <property type="entry name" value="Histone deacetylase complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1800.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024145">
        <property type="entry name" value="His_deAcase_SAP30/SAP30L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038291">
        <property type="entry name" value="SAP30_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025718">
        <property type="entry name" value="SAP30_Sin3-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025717">
        <property type="entry name" value="SAP30_zn-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13286:SF6">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT SAP30L-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13286">
        <property type="entry name" value="SAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13867">
        <property type="entry name" value="SAP30_Sin3_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13866">
        <property type="entry name" value="zf-SAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Histone deacetylase complex subunit SAP30 zinc-finger" evidence="12">
        <location>
            <begin position="21"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Histone deacetylase complex subunit SAP30 Sin3 binding" evidence="13">
        <location>
            <begin position="109"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="20446" checksum="0A7E9EF50C8B3FB4" modified="2018-12-05" version="1">MNGFSTEEDSHDGPPAPPFYGQSCCLIEDGERCGRSAGNASFSKRIQKSISQKKLKLDIDKSVRHLYICDFHKNFIQSVRNKRKRKTSDDGGESPDHDVEVPEVDLFQLQVNTLRRYKRHYKLQTRPGLNKAQLAETVSRHFRNIPVNEKETLTYFIYMVKSSKSRLDQKADAGKPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MY78</accession>
    <name>A0A3B5MY78_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Runt-related transcription factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">Interacts with DDX5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MY78"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026822"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027151.1">
        <property type="protein sequence ID" value="ENSXCOP00000026822.1"/>
        <property type="gene ID" value="ENSXCOG00000020028.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166083"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007178">
        <property type="term" value="P:cell surface receptor protein serine/threonine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002062">
        <property type="term" value="P:chondrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
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    <sequence length="442" mass="48003" checksum="9B408E6FA6DFD604" modified="2018-12-05" version="1">MRIPVDPSASRRFSPPSSSLQPVPGKMNDVSSPAGQPDAAAAVPRLRPPHENRSMAEIIADHPAELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGDIPDGTVVTVMAGNDENYSAELRNASGVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYHRAIKVTVDGPREPRRHRQKLEDPPKTGLFSDRLSELERMRVRVTVPTQTPRPALNTAANTFNPQGQTQITDPRQSQSSPPWSYEQPYPSYLSPMASPSVHSTTPLSSSRGTGLPAIRSSDLSPFPGQFERQFPSLSSITDSRFSSPRMHYPATFTYTPPVTSAMTLGSAHYHTYLPPPYPGSTQSQSGPFQTSSTPYLYYGASSNSYQFSMVPGGDRSPSRMVPPCTSASTGTSLVNPNLPSQTEGGVDGDGSHSNSPTVLNPGGRMDEAVWRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Neoteleostei</taxon>
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        <source>
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    </evidence>
    <sequence length="256" mass="29479" checksum="B54D898BB42468E3" modified="2018-12-05" version="1">MHSLDIFSTPSPKQVQRADRRDTSKNKKNAQILSIFPVTQEPSIITASRLLGHHGLFNHEVKSISIERVLSEQSKLEQNEEKTNEENNNTPYLSSSPHNPILLSNNVSGPELWETNKCKTQESDLLDSFQNKFVNQTRNLRERLSAQQPHHSNAQPFFSHQVRHSADLHGFPQEQNPSKTDRFYFAPFFPSKSHRQPQSNHLQAFGQFSPVHQNFRPDPTDMMHYPPSHMLERNLAPTSSSLPSPAHWSFPPMRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
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        <name type="scientific">Xiphophorus couchianus</name>
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    </dbReference>
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    <keyword evidence="8 15" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0306">Gastrulation</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14 15" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="53"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000028017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="392" mass="43964" checksum="F808E465FE7A1DC8" modified="2018-12-05" version="1">MSGDNYTYIKMSDFPTSSTPTPSLDHCDYDDWYPSFSIIPSVYVLAFLVGCLGNSLVLSVYLASRQHLPSPYSLIASLALADLCFLVTLPLWATYTAMGYHWPFGQPLCQISSFLTALNMYASVFSLSTLSVERYWVLTGRRHSGHHPLTRCPSRTLWILVAVWVVAAVLALPGLLLRSVREVELDPKSEDDPGSVVLSCQMDYSMLVGAELEEAEKDRAEVWWAAVLSLKSTLIGFLLPLVIMLVCYCSLAQLLSKHFGHGPRPDRRRQRRLLRVIVTLVTAFFLCWMPLNVNKTVSILLELGFVPYSCSLDQALLAAHPYVTCLAYLNSCLNPLLYAACDPSFRERCRATLLTCCGMCRRELLLILHSSLFMKLLHSSSVFSQRRLPDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KLQ5</accession>
    <name>A0A3B5KLQ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Golgi integral membrane protein 4a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KLQ5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001043"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001056.1">
        <property type="protein sequence ID" value="ENSXCOP00000001043.1"/>
        <property type="gene ID" value="ENSXCOG00000000864.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164637"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042336">
        <property type="entry name" value="GOLIM4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22909">
        <property type="entry name" value="GOLGI INTEGRAL MEMBRANE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22909:SF24">
        <property type="entry name" value="GOLGI INTEGRAL MEMBRANE PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="91"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="18436" checksum="0510187F595FD6AE" modified="2018-12-05" version="1">MGNGVCSRRQRRIFQCLLLVTVVCGMMYGGLMSYEMHKQLRRTEATALKYQQHQESLSAQLQVYEHRSRLEKSLQKERLEHKKAKEYLGYKLEAEQSLNKEKNQHDELKRQYYELNEQHQVQGEDHSRLLDEHRDRYNKLQQAKEVEVSQLKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQV8</accession>
    <name>A0A3B5KQV8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA wybutosine-synthesizing protein 3 homolog</fullName>
            <ecNumber evidence="3">2.1.1.282</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36635"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.1.1.282"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TYW3 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.282" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQV8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000857"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000867.1">
        <property type="protein sequence ID" value="ENSXCOP00000000857.1"/>
        <property type="gene ID" value="ENSXCOG00000000715.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153304"/>
    <dbReference type="UniPathway" id="UPA00375"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1960.10:FF:000001">
        <property type="entry name" value="tRNA wybutosine-synthesizing protein 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1960.10">
        <property type="entry name" value="tRNA wybutosine-synthesizing-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003827">
        <property type="entry name" value="tRNA_yW-synthesising"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036602">
        <property type="entry name" value="tRNA_yW-synthesising-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418:SF1">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02676">
        <property type="entry name" value="TYW3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111278">
        <property type="entry name" value="SSo0622-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA wybutosine-synthesizing protein" evidence="12">
        <location>
            <begin position="9"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="255" mass="29071" checksum="854D669989B3B8CC" modified="2018-12-05" version="1">MDRTFSRIKTQSLNRLDLSKKGSVDEDIEHVVSLLNSCEQFFTTSSCSGRIILIDGAAGSSDAHKQNCVWLFVSHQKCTCDDLVRYSLSRACGDAVLKFEPFVLHVQCRRLEDAQLLHSVAVNSGFRNSGLTVGKTGKIVSAVRSTHGLEVPLSRHGKLLVDEDYIQFLTQIANQKMEENLRRVQRLAFWSLQENQHHLIFGPLDVLCSLRSSSCYRRRVRYLLRPPGAPKSNFFFFFLIHKIMISTYITKYILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KR19</accession>
    <name>A0A3B5KR19_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM110 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KR19"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000081"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000081.1">
        <property type="protein sequence ID" value="ENSXCOP00000000081.1"/>
        <property type="gene ID" value="ENSXCOG00000000066.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183056"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025740">
        <property type="entry name" value="FAM110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025741">
        <property type="entry name" value="FAM110_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025739">
        <property type="entry name" value="FAM110_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14758">
        <property type="entry name" value="AGAP005440-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14758:SF4">
        <property type="entry name" value="PROTEIN FAM110A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14160">
        <property type="entry name" value="FAM110_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14161">
        <property type="entry name" value="FAM110_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centrosome-associated FAM110 N-terminal" evidence="4">
        <location>
            <begin position="13"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Centrosome-associated FAM110 C-terminal" evidence="3">
        <location>
            <begin position="310"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="292"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="368"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="183"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="439" mass="47416" checksum="530929F3766CA1E3" modified="2018-12-05" version="1">MPVETLRPSDGRLAGVAFTSGRPFRILNKGPNYFRPPAEPGARKLSAVERLEADKAKYVKSQQVALTRQAPIQAPLIRKPLVPPVMMLQGSVGAPTARKVPRCPADVENRGGGVVLGERRPFNLDILNNLINDVCDGAQSSSSTSPSSSSPSSGVKSICSSLSAEQDKSNRLVNNLKPLNHGTNNSSSTSSCTSSLLSNNRCVPAAELSRRPPPIPARVVRGVQAPYNAPNSVTVRRVDVRPHAEVKKPPRVALHPHLRPRQIPQGQVAHSQLPPPPPPMLVRAGIIPPASPAFTRISNASSKGSSRKHPSLHRSKSDLSDRYSRATADLERFFNYCGLDPEEVEGLGGVERFTRANSDIVSVSKLRSVSTPSSECGDDVERIRGYGGGDEEEDDEDGPLRTNDRVPYGISVIERNARVIKWLYGIRQAREANSGVSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRN4</accession>
    <name>A0A3B5KRN4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRN4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000550"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000559.1">
        <property type="protein sequence ID" value="ENSXCOP00000000550.1"/>
        <property type="gene ID" value="ENSXCOG00000000477.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155724"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070139">
        <property type="term" value="F:SUMO-specific endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090234">
        <property type="term" value="P:regulation of kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090169">
        <property type="term" value="P:regulation of spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051947">
        <property type="entry name" value="Sentrin-specific_protease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46896">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46896:SF1">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="26"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="322" mass="36791" checksum="83DC6AE1220AD939" modified="2018-12-05" version="1">MTDLQPTFSGPVMLMVYPPPPAKGGISVTNEDLHCLNDGEFLNDVIIDFYLYLVLERLKKEDAQRIHVFSSFFYKRLNQRERRSGPDTVNLIQKRKHNRVKTWTRHVNLFQKDFIFVPINEAHWYLAVICFPGLDGPVLEKNPMYLDPPAASGSPAELQLEENIPDHDECSDDGNAADDTGACETSLASKPVTCKPCILIMDSLRGPARSNVVKTLRYLEVEWEVKQGTQRGFGKEVMKGSSPRVPQQDNFSDCGVYVLQYVESFFENPIPSFHLPMNLSDWFPQQRMKTKREEIKELILKIKDQQEVDRKESGRAEEPPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTD3</accession>
    <name>A0A3B5KTD3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">G protein-coupled receptor 63</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GPR63</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTD3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001230"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001248.1">
        <property type="protein sequence ID" value="ENSXCOP00000001230.1"/>
        <property type="gene ID" value="ENSXCOG00000001009.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183001"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000080">
        <property type="entry name" value="probable G-protein coupled receptor 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051880">
        <property type="entry name" value="GPC_Orphan_Receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24245">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24245:SF1">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 63-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="269"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="55"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="378" mass="42680" checksum="1188FB1F4A467531" modified="2018-12-05" version="1">MEGVSMANMSLGSGSSPQPVAPTEKSLAIAPGLGLPLQVFFSLVMATILLVALAGNVVVCLMVYQRSAMRSAINILLANLAFADMMLAIFNMPFALVTVMTTTWIFGNTFCRLSAMLFWLFVIEGGAILLIISIDRFLIIVQKQDKLSPQKAKLLIVITWVLSSVFSSPLAVGSPRLQIPPRAPQCVFGYSADPGYHAYVLILMLVFFFIPFLVMLYTFMGILNTIRHNAIRIHSHPDSMCLSQASKLGLLSLQKPFQMNIDMSFKTRAFTTILILFSIFTVCWAPFTTYSLVTTFSFGFYHKDSFFQISTWLLWLCYLKSALNPLIYYWRIKKFRDIKYSQSEREKMKNRPELPELQVPACGHCFFQFPKVHCLSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KVJ3</accession>
    <name>A0A3B5KVJ3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KVJ3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001631"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001656.1">
        <property type="protein sequence ID" value="ENSXCOP00000001631.1"/>
        <property type="gene ID" value="ENSXCOG00000001325.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159747"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="10">
        <location>
            <begin position="28"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="216" mass="25135" checksum="75F271D3FDFE984E" modified="2018-12-05" version="1">SAAAGIFLLNIFYSSVSSLYFHIGETEKKCFIEEIPDETMIIGNYRTQLYDKQKEEYMPATQGLGMFVEVKDPDDKVILSRQYGSEGRFTFTSHTPGEHQICLHSNSSKFSLFAGGMLRVHLDIQVGEHANNYAEIAAKDKLTELQLRVRQLVEQVDQIQKEQNYQRYREERFRQTSESTNQRVLWWSIVQTLILVAIGIWQMRHLKSFFEAKKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KX30</accession>
    <name>A0A3B5KX30_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">STK38L</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 23">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027899979.1">
        <property type="nucleotide sequence ID" value="XM_028044178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KX30"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000129"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000130.1">
        <property type="protein sequence ID" value="ENSXCOP00000000129.1"/>
        <property type="gene ID" value="ENSXCOG00000000115.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114161113"/>
    <dbReference type="GeneTree" id="ENSGT00940000153544"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05627">
        <property type="entry name" value="STKc_NDR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000057">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000086">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000118">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF76">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_135, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="90"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="20">
        <location>
            <begin position="384"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="463" mass="53738" checksum="DA71755C741DBD59" modified="2018-12-05" version="1">MAMTGGTAAALPMSNHTRERVTVAKLTLENFYSTLLTQHEEREMRQKKLEKAMEEEGLPDEEKVMRRSQHARKETEFLRLKRTRLGLDDFESLKVIGRGAFGEVRLVQKKDTGHIYAMKILRKADMLEKEQVAHIRAERDILVEADSAWVVKMFYSFQDKRNLYLIMEFLPGGDMMTLLMKKDTLSEEATQFYIAETVLAIDSIHQLGFIHRDIKPDNLLLDSRGHVKLSDFGLCTGLKKAHRTEFYRNLTHNPPSDFSFQNMNSKRKAETWKKNRRQLAYSTVGTPDYIAPEVFMQTGYNKLCDWWSLGVIMYEMLIGYPPFCSETPQETYRKVMNWKETLVFPPEVPISEKAKDLILRYCTDAENRIGAVSVEEIKSHPFFESVDWEHIRERPAAISIEIKSIDDTSNFDDFPESDILQPANATEPDFKSKDWVFLNYTYKRFEGLTQRGTIPTYMKAGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KX98</accession>
    <name>A0A3B5KX98_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GDP-L-fucose synthase</fullName>
            <ecNumber evidence="4">1.1.1.271</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Fucose synthase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.271" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027884293.1">
        <property type="nucleotide sequence ID" value="XM_028028492.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027884295.1">
        <property type="nucleotide sequence ID" value="XM_028028494.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027884296.1">
        <property type="nucleotide sequence ID" value="XM_028028495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KX98"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004728"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004785.1">
        <property type="protein sequence ID" value="ENSXCOP00000004728.1"/>
        <property type="gene ID" value="ENSXCOG00000003722.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114151342"/>
    <dbReference type="KEGG" id="xco:114151342"/>
    <dbReference type="GeneTree" id="ENSGT00390000004681"/>
    <dbReference type="UniPathway" id="UPA00128">
        <property type="reaction ID" value="UER00191"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050577">
        <property type="term" value="F:GDP-L-fucose synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042351">
        <property type="term" value="P:'de novo' GDP-L-fucose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05239">
        <property type="entry name" value="GDP_FS_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00956">
        <property type="entry name" value="GDP_fucose_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028614">
        <property type="entry name" value="GDP_fucose/colitose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43238">
        <property type="entry name" value="GDP-L-FUCOSE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43238:SF1">
        <property type="entry name" value="GDP-L-FUCOSE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="9">
        <location>
            <begin position="3"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="315" mass="35586" checksum="3A4C10D4280841EF" modified="2018-12-05" version="1">MKVLVTGGSGLVGRAIRHVIEEEGGAKKGEEWIFLSSKDANLMNKEETQAVFEKHRPTHVIHLAAMVGGLFKNMKHNLDFWRNNIYINDNVLQAAHEVGTVKVVSCLSTCIFPDKTTYPIDETMIHNGPPHESNFGYAYAKRMIDVHNRAYYQQHGRSYTAVIPTNVFGPHDNFSIEDGHVLPGLIHKAYIAQKEGKPLVVWGSGSPRRQFIYSLDLARLFLWVLREYPEVDPIILSVGEEDEVSIKEAAEAVVESLSFKGEVVYDTSKADGQFKKTASNAKLHQYLPDFKFTPFRQALKETCDWFVANYDAARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYU1</accession>
    <name>A0A3B5KYU1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KYU1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000719"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000729.1">
        <property type="protein sequence ID" value="ENSXCOP00000000719.1"/>
        <property type="gene ID" value="ENSXCOG00000000607.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164311"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21066">
        <property type="entry name" value="NURR_hnRNPQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000024">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein q isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041337">
        <property type="entry name" value="hnRNP_Q_AcD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21245">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18360">
        <property type="entry name" value="hnRNP_Q_AcD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="166"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="252" mass="27622" checksum="BD622C3290F05C10" modified="2018-12-05" version="1">MSADLATDHVNGNGTEEPMETMATVTRSEHFQALLDAGLSQNVAEKLDELYVAGLVAHSDLDERALEALKEFNEDGALQVLVQFKESDLSHVQNKSAFLCGVMKTYRQREKQGTKVSPASKGPDEVRIRELLDRTGYTLDVTTGQRKYGGPPPDAVYAGSQPTVGTEIFVGKIPRDLFEDELVPLFEKAGPIWDLRLMMDPLSSLNRGYAFVTFCAKDSAQEAVKLVGPQAARCTWTQPGPVPQPLLSLLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZG8</accession>
    <name>A0A3B5KZG8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zinc phosphodiesterase ELAC protein 2</fullName>
            <ecNumber evidence="5">3.1.26.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ElaC homolog protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Ribonuclease Z 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">tRNA 3 endonuclease 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">tRNase Z 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Zinc phosphodiesterase, which displays mitochondrial tRNA 3'-processing endonuclease activity. Involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA. Associates with mitochondrial DNA complexes at the nucleoids to initiate RNA processing and ribosome assembly.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="19">Homodimer. Interacts with PTCD1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
            <location evidence="3">Mitochondrion nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RNase Z family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZG8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005483"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005545.1">
        <property type="protein sequence ID" value="ENSXCOP00000005483.1"/>
        <property type="gene ID" value="ENSXCOG00000004279.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111191"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990180">
        <property type="term" value="P:mitochondrial tRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047151">
        <property type="entry name" value="RNZ2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027794">
        <property type="entry name" value="tRNase_Z_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553:SF49">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13691">
        <property type="entry name" value="Lactamase_B_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1135">Mitochondrion nucleoid</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="21" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017188431" description="Zinc phosphodiesterase ELAC protein 2" evidence="21">
        <location>
            <begin position="20"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="tRNase Z endonuclease" evidence="22">
        <location>
            <begin position="54"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00046098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF13691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="151" mass="16870" checksum="56593A7663A0B822" modified="2018-12-05" version="1">HLALCSLAWCSFGTCMGSAFSAKRPQKEPLRRLKTKESRDKRGDVHGPSNVYVQVVGAGSRDSAASLYVFSEYNRYLFNCGEGTQRLMQEHKLKAARLDNIFLTRLSWENVGGLSGMILTLKDTGVPECVLSGPPQLLFDRTLRRCTKMKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L046</accession>
    <name>A0A3B5L046_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L046"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004172"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004218.1">
        <property type="protein sequence ID" value="ENSXCOP00000004172.1"/>
        <property type="gene ID" value="ENSXCOG00000003282.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156133"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030837">
        <property type="term" value="P:negative regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043587">
        <property type="entry name" value="Phosphatase_SSH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45864:SF7">
        <property type="entry name" value="PROTEIN-SERINE_THREONINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45864">
        <property type="entry name" value="SLINGSHOT PROTEIN PHOSPHATASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="112" mass="13006" checksum="440554BEE886DAEF" modified="2018-12-05" version="1">FTWIHIQRAASPCGESFCDSEGDLSHVIRFRPFVLLFSSISESFFMVKGAALFLQQGSNTQGPKTPTHHKHAGDLPQHLQVMFKILRSEDRIKLVSFLFFYKHKHHYIYVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L0B2</accession>
    <name>A0A3B5L0B2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Myozenin 3a</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the myozenin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L0B2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004237"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004284.1">
        <property type="protein sequence ID" value="ENSXCOP00000004237.1"/>
        <property type="gene ID" value="ENSXCOG00000003328.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183027"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051373">
        <property type="term" value="F:FATZ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031433">
        <property type="term" value="F:telethonin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008438">
        <property type="entry name" value="MYOZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15941">
        <property type="entry name" value="MYOZENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15941:SF16">
        <property type="entry name" value="MYOZENIN 3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05556">
        <property type="entry name" value="Calsarcin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="214" mass="24294" checksum="956EF4E473FCBCB2" modified="2018-12-05" version="1">MYAFHSQNESVNHLLLCSLLTGLNLGKKISVPKDVMMEELNLSSNRGSRMFQERQRRAEKFTLENVANGPYNPNVRNRKEQTHCKMLKYSFFPESLSKKGNPNVLAPGYSGPLKEIPHEKFNTTVIPKSYCSPWREALGGSEELQPANYRCFNRTPMPFGGTMASKRVIPVIGFEAVEPQKLPGVALDRMCRRPNFNRAPRGWGIDYSPESNEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2E1</accession>
    <name>A0A3B5L2E1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Dual specificity protein phosphatase</fullName>
            <ecNumber evidence="9">3.1.3.16</ecNumber>
            <ecNumber evidence="9">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="9"/>
    <dbReference type="EC" id="3.1.3.48" evidence="9"/>
    <dbReference type="RefSeq" id="XP_027897307.1">
        <property type="nucleotide sequence ID" value="XM_028041506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2E1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004520"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004576.1">
        <property type="protein sequence ID" value="ENSXCOP00000004520.1"/>
        <property type="gene ID" value="ENSXCOG00000003573.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114159540"/>
    <dbReference type="KEGG" id="xco:114159540"/>
    <dbReference type="CTD" id="1848"/>
    <dbReference type="GeneTree" id="ENSGT00940000158342"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033550">
        <property type="term" value="F:MAP kinase tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008330">
        <property type="term" value="F:protein tyrosine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009953">
        <property type="term" value="P:dorsal/ventral pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905179">
        <property type="term" value="P:negative regulation of cardiac muscle tissue regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070373">
        <property type="term" value="P:negative regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042663">
        <property type="term" value="P:regulation of endodermal cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040036">
        <property type="term" value="P:regulation of fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060420">
        <property type="term" value="P:regulation of heart growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01446">
        <property type="entry name" value="DSP_MapKP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14566">
        <property type="entry name" value="DSP_MKP_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000011">
        <property type="entry name" value="Dual specificity phosphatase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000011">
        <property type="entry name" value="Dual specificity phosphatase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008343">
        <property type="entry name" value="MKP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF45">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000939">
        <property type="entry name" value="MAPK_Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01764">
        <property type="entry name" value="MAPKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="14">
        <location>
            <begin position="31"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="12">
        <location>
            <begin position="211"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="13">
        <location>
            <begin position="267"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="196"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="10">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000939-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="388" mass="43209" checksum="F9B51D7C85559C47" modified="2018-12-05" version="1">MLDKLKPVIQLDSVMAISKTVGWLREQLETRRDGLLVMDCRAQELYESSHVETAINVAIPSLMLRRLKKGNLPVRSLLSDGEDRESFVRRCKTDTIVLYDEYSREWNENVDGGSVLGLLLRKMKDEGYKAFYLEGGFSKFQAEFPALCETNLDGSSSSSSSSSSSSPTTAQVLGLGGLRISSDSSDIESDFDPSSATDSDGSPLSNPQPSFPVEILPHLYLGCAKDSTNLDVLEEYGIKYILNVTPNLPNLFENAGEFKYKQIPISDHWSQNLSQFFPEAISFIDEARGQKCGVLVHCLAGISRSVTVTVAYLMQKLNLSMNDAYDIVKMKKSNISPNFNFMGQLLDFERTLGLKSPCDNRMDPPSQQLYFTTPTNHNVFQLDPLQST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2N3</accession>
    <name>A0A3B5L2N3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Heat shock protein family A (Hsp70) member 12A.2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2N3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002044"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002074.1">
        <property type="protein sequence ID" value="ENSXCOP00000002044.1"/>
        <property type="gene ID" value="ENSXCOG00000001646.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154551"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA12"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="ATPase_NBD"/>
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    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
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        <property type="entry name" value="ALPHA KINASE/ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187:SF5">
        <property type="entry name" value="HEAT SHOCK 70 KDA PROTEIN 12A"/>
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        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002044.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="559" mass="64121" checksum="5E562CF9F0C849B5" modified="2018-12-05" version="1">MGDSFIIAIDFGTAYSGYAFNITPRNQKTEPCVRYWGEKSARETPKTPTCILFDKYKQFISFGYEAKEAYVSRMGTEAKDKLFFESFKMSLYKRVTANGKEMKALQVFTEALKFLKEDALTTIAQHTAGRQFTASDFTWVLTVPAIWEESAKQFMREAATQAGIVTKGKEDKLVIALEPEAASVYCKKLPSDGFIAENLGDGKLDQSPGTQYIVVDCGGGTIDITFHEVLEEGNLKELHKASGNDLGGQTVDRKFKQFLQEIFTGVWDDYEKKFPNEAQKIMYDFTLFKKHEDDVEIPCPFNLGKLAEKKKKIEKYFKKVHGATWEDGSIKISKEKLREFFEESLQGITRCLREILEKDFKIKYILLVGGYADSKILRNHITDEFGDDYIILCPFRPQEVIMKGAVEFGRNKGVVASRKNPFTYGVGIFQRFDKFRHREDKKFTNKDGVWCRDIFMKLVDVGEDVRWNETREHTLYPVEAAQKAMSFSFYRTKKKHVAYIDEPDTEEIGSFIVESPDTTLGLNREIRLKIKFGFTEMTATGTDRESGSSQTIRINFMRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L577</accession>
    <name>A0A3B5L577_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Neudesin neurotrophic factor</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L577"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005560"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005626.1">
        <property type="protein sequence ID" value="ENSXCOP00000005560.1"/>
        <property type="gene ID" value="ENSXCOG00000004337.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162504"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF72">
        <property type="entry name" value="NEUDESIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017231401" evidence="8">
        <location>
            <begin position="27"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="9">
        <location>
            <begin position="43"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="168" mass="18985" checksum="17002C069DBAE07C" modified="2018-12-05" version="1">SGRRGGWWWVFLHVWVLFVLLSVSLAEEVKLNYKPATKPVRLFTEEELQRYDGSEEGQPIYMAIKGVVFDVSKGKEFYGKDASYNALVGKDSTRAVAKMSLNPADLTSDTTGLTEEQLQSLDSVFEGTYKAKYPIVGYTAARLLNADGSPNKDFRPEDQPRFQTRDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L605</accession>
    <name>A0A3B5L605_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SAM and SH3 domain containing 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L605"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005835"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005901.1">
        <property type="protein sequence ID" value="ENSXCOP00000005835.1"/>
        <property type="gene ID" value="ENSXCOG00000004521.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156778"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014002">
        <property type="term" value="P:astrocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036269">
        <property type="term" value="P:swimming behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd11967">
        <property type="entry name" value="SH3_SASH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000024">
        <property type="entry name" value="Putative sam and sh3 domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000021">
        <property type="entry name" value="Putative sam and sh3 domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051725">
        <property type="entry name" value="SAM-SH3_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058666">
        <property type="entry name" value="SASH1/NUB1_homeodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035720">
        <property type="entry name" value="SASH1_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021090">
        <property type="entry name" value="SPIDER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12301:SF3">
        <property type="entry name" value="SAM AND SH3 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12301">
        <property type="entry name" value="SAM-DOMAIN, SH3 AND NUCLEAR LOCALIZATION SIGNALS PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26285">
        <property type="entry name" value="SASH1_Homeodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12485">
        <property type="entry name" value="SPIDER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="548"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="6">
        <location>
            <begin position="627"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="6">
        <location>
            <begin position="1087"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="186"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="445"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="612"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="692"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="857"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="204"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="463"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="499"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="518"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="616"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="941"/>
            <end position="959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1156" mass="127874" checksum="3FD1F1E37E9698A2" modified="2018-12-05" version="1">MEGDQGLDAFSEEPQTDTAAASDSFSQLWTDVMGMLDGSLGNIDDLAQEYSEYYNTCFSDVSDRMEELRKRQVSQELDMVSHCTCNSTDFIFLFFYFFLCRMPLHKSSSEDGSGGKWDNKKKNKSFWQNFRKSQHKPVARQSSKGEDIGYVASEITMSDEERIQLMMMVKEKMITVEEALARLKEYERSKQSSSTDTAEWTEGSAANLNLSSNCNSREQSDDEQSEDSVKFKRLHKLVNSTRRVRKKLIKVEEGKKHSPEGFLNLETPSCEDNTALYTGVLKKPPLPQEASLPSLIHDQLSLDGDTDSLTTSPSSSSLDTWSGHKLVKTFSKSSSTHGLIRPPRRTAAGSRDLGGSISGVAGSGSSFSELDGCGLDDDGKLSRSTTDSEMRKALSSISHGRTCSFGGFDLSNRSLHVVNAASENKEQEAIYREVVKSPNTTRISLGKKVKSVKETMRKRMSKKYSSSLSEQSSPDGAPGSPQSPLPDTDSLEKPKLKAGGSVESLRSSLSGQSSMSGQTVSTTDSSASNRESVKSEDGDDEEPPYRGPFCGRARVHTDFTPSPYDTDSLKLKKGDVIDIISKPPMGTWMGLLNNKVGTFKFIYVDVLSEEEEKPKRPIRRRRKGRPPKPTSVEELLDRINLKEHMPTFLFNGYEDLDTFKLLEEEDLDELNIKDPQHRAVLLTAVELLQEYDSSSDPERSGLSGSQEKLLSEGRGLVGDSPRDSGCYESNENLENGKNRKASRSSRSSAGMQSPDYPTLPMTLSTEALQQNSKNQRSKFPKSFFIKPSLKGFNLLGLRKTQKHSPIPASRSCEDLDGLPQPSGLWKRSHSLGDLHNLQQKDDLNVVVKTAKDVANSPVKVYREERSPTHKRTPAVSPKGRSVRPPIPSQLPLQPLSSPTPSPPDSSASGERIILTHPKKPPIPPPVPAKKSRERLVNGVRHPSLSLPSSPSPTASPTHSVNRSQPSREDSGSPPAVQPPWLSDLGGKMAVTRKLSHTKMSPDLHTLLEQRLQAESIDLTEEPYSDKHGRCGIPQALIQRYSEDLEQSVKDVASSMDQLRVKELRKQHRMAIPSRGLTEMSRKPVNLGNISTVFDWLVYIGLPMYGPSLAAAGVDTLSRVALLTESSLRDAGVRDERHTRRLISEARLVNTHRGMQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6F8</accession>
    <name>A0A3B5L6F8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">G protein signaling modulator 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GPSM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6F8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003359"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003397.1">
        <property type="protein sequence ID" value="ENSXCOP00000003359.1"/>
        <property type="gene ID" value="ENSXCOG00000002653.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154667"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001965">
        <property type="term" value="F:G-protein alpha-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002932">
        <property type="entry name" value="Adenylate kinase isoenzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000043">
        <property type="entry name" value="G-protein-signaling modulator 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003109">
        <property type="entry name" value="GoLoco_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052386">
        <property type="entry name" value="GPSM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45954:SF2">
        <property type="entry name" value="G-PROTEIN-SIGNALING MODULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45954">
        <property type="entry name" value="LD33695P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02188">
        <property type="entry name" value="GoLoco"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00390">
        <property type="entry name" value="GoLoco"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50877">
        <property type="entry name" value="GOLOCO"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="223"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="402"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="466"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="561"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="595"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="470"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="623" mass="69910" checksum="638621DC7B3349E1" modified="2018-12-05" version="1">MEASCLELALEGERLCKAGDFKGGTAFFEAAVQVGTEDLKTLSAIYSQLGNAYFYLKEYGKALEYHKHDLTLARTIGDRIGEGKASGNLGNTLKVLGRFDEAVVCCQRHLDISQEQSDKVGEARALYNIGNVFHAKGKQQLWGCTQEPGELPSNVRETLQKATGFYEMNLCLVKELGDRAAQGRAYGNLGNTHYLLGNFVEAIKFHRQRLSIAKEFGDKAAERRAYSNLGNALIFLGQFNTATEYYRKTLQLSRQLRDQVMEAQACYSLGNTYTLLQQYERTIDYHLKHLCIAQELNDRVGEGRACWSLGNAYVSLGNHKQALHYARKHLDISKEIGDRNGELTARMNVEQLMEALGVNESDLSPSSSEFEMQGNTQKVDCFLRNMDDYWFFKRSRNQLSQAGKRKGYSDSQSSDERPWLDSPVDTDDITVQVTPPKLGRDPSDEDCFFDLLSKFQSSRMDDQRCHLDETQNGDNTEGAANTSLSEMIDPSITTSPQTEELFDLIASSQSRRLDDQRVSVGNLPGLRITHNNLGHLVGEGDHQEPSDDFFNMLIKCQSSRIDDQRCSPPEAGPHAPTVPDEDFFSLIQRVQAKRMDEQRVQLPSDDQDSSNSPDQEPPGDFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6W8</accession>
    <name>A0A3B5L6W8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6W8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005521"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005587.1">
        <property type="protein sequence ID" value="ENSXCOP00000005521.1"/>
        <property type="gene ID" value="ENSXCOG00000004313.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155581"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015175">
        <property type="term" value="F:neutral L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Y+L amino acid transporter 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF519">
        <property type="entry name" value="LARGE NEUTRAL AMINO ACIDS TRANSPORTER SMALL SUBUNIT 1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017191099" evidence="10">
        <location>
            <begin position="21"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="289"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="411"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="446"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="474"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007040"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
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        <source>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005521.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="528" mass="57385" checksum="D5C37806830BE7B7" modified="2018-12-05" version="1">MCFTLLIFCSAVLTSPATEAEPNQDGLIVEHPASPSDPMVSAPAATDPTDSLPGKGEKIKLKRSITLFNGVGMIIGTIIGSGIFVTPTGVVKETGSAGLSLIVWAACGVVSTMGALCYAELGTTIAKSGGDYTYILEVYGELAAFLKLWVEMLIIRPSSQYVVSLVFATYLLKPLYPTCPVPDSAAKLIACVCLTSLTFVNCISVRAATKVQDLFTASKLLALIIIILFGFIQISTGDVPYLTPEKAFEGSKMGVDNIVLALYSGLFAFGGWNYLNYVTEEMINPERNLPLAIIISMPIVTVVYVLTNLAYFTTISPQVMVESEAVAVKFGEYHLGVMAWLIPVFVGLSCFGAVNGSLFTSARLFYAGAREGQLPAALGLVHTDLFTPVPSLIFTCLLSMMYAISQDIFSVINLFSFFTWLCVGMAIAGLVWLRITKPDLRRPIKVCLFIPVTFVLGCVFMIVVSFWAAPFESLVGSSIILTGIPAYLLGYKWKKPHMVKKMLEIFTMFCQKIFMSVPEEKEKHEQAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L898</accession>
    <name>A0A3B5L898_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dehydrogenase/reductase (SDR family) member 13b.1</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L898"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006680"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006762.1">
        <property type="protein sequence ID" value="ENSXCOP00000006680.1"/>
        <property type="gene ID" value="ENSXCOG00000004193.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="327" mass="36114" checksum="D9DBEC427CBDF8E7" modified="2018-12-05" version="1">MIFSFCGWCRTMSVVLLVVGVVIGVAYIYRHIVVKGKRCTSKAKLHGKTVIVTGSNTGIGKTTAIDLARRGARVILACRCKQRGEAAMEEIRRESGSSQVVFMQLDLGSLKSIRSFAESFLKSEPRLDILINNAGRTGQTEDGFGLMFGVNHLGHFLLTNLLLDRLKECAPSRIINVSSMAHNFGKIDFDCLNSHKALGLGTSFIRLLQIYCDSKLCNALFNHELAKRLRGTQVTCYTLHPGAISSELGRNTGLFLQIVLKPITTFFFKNTVQGCQTTLHCALQEGIEPLSGRYFSNCTVRDLFAKARDDAAAKKLWEISERLCGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8A1</accession>
    <name>A0A3B5L8A1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSXCOT00000004063.1">
        <property type="protein sequence ID" value="ENSXCOP00000004019.1"/>
        <property type="gene ID" value="ENSXCOG00000003157.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="TMEM109"/>
    </dbReference>
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        <property type="entry name" value="TRANSMEMBRANE PROTEIN 109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14550:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 109"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BRI3BP"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017458983" evidence="2">
        <location>
            <begin position="21"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="212" mass="22995" checksum="E4723E1F694B1DD6" modified="2018-12-05" version="1">MARVWFSVATLVVGVSLCWAGGQQAKAESPPVVTLQCCPSSLLQKHASHSFPSLSFPSPPKFLSTAIRFLAEGAASGLNVIAVYVTEILRVTGFDVATLPRFTPEGVTAVAQWGLVGLIAYWSLTILLRLLISVFKQLFWVVKTVLALWLFGLIVTDKHASAETTAIRVGVLVLVVALLTLFTSSSEKTSGLESRLNKLEGRLRAVEKRKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8F6</accession>
    <name>A0A3B5L8F6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8F6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008578"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008684.1">
        <property type="protein sequence ID" value="ENSXCOP00000008578.1"/>
        <property type="gene ID" value="ENSXCOG00000006567.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167777"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050488">
        <property type="entry name" value="Ig_Fc_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481:SF64">
        <property type="entry name" value="FC RECEPTOR-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481">
        <property type="entry name" value="IMMUNOGLOBULIN FC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="298" mass="33177" checksum="843F5E60C6FB13E7" modified="2018-12-05" version="1">MQWASPGASVTLSCEVKPSSPGWKFYWYKAVPEQPDTYKYLPLNEGTHDTLQDSYVVHDQKHTAGYACRAGWGVPEYFTNYSEPKIVWSADSHPSAALTVSPARDNLYTVDEVQLTCTGSSGVWRVRRFRPYYQSDVSDCSVWGTMTESSCIFSSPQYHKGVYWCESGSGEFSNAVNITIHGKFTYVFLNFFLLSNFIPCCRGRLLTVAYIQSSTGTKPFPICRRTSTDTSCCLTASAGPLRRPTSSTERRTRLCTPAGLEEKKGSISPITVNQSLSGPQVSVLLFNKLNLGSRVRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9G9</accession>
    <name>A0A3B5L9G9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9G9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006396"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006476.1">
        <property type="protein sequence ID" value="ENSXCOP00000006396.1"/>
        <property type="gene ID" value="ENSXCOG00000004954.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165326"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043593">
        <property type="entry name" value="ASAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854:SF1">
        <property type="entry name" value="ARF-GAP WITH SH3 DOMAIN, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45854">
        <property type="entry name" value="ASAP FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAR" evidence="1">
        <location>
            <begin position="45"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="207" mass="24097" checksum="FF8EBE115B7A67F4" modified="2018-12-05" version="1">FQGEIFFKENGALNEIKKKGKLEKERREKARQLGVIRRDGSDGGEDMERERRIFQLQMCEYLLKIQELKVRQGPDLLQSLIKYFQAQLNFFQDGLKAAENLTPFIEKLASSIHTMRLEQDEEVKKLLQLRDSLRLLLQVEGKEEYLNRKNSGNGYSIHQPQGNKKYGTEKSGFLQKKSDGYINSSGFNKSIIFIRIAHTAFVSFRRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9P5</accession>
    <name>A0A3B5L9P5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Elongation of very long chain fatty acids protein 6</fullName>
            <ecNumber evidence="14">2.3.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">3-keto acyl-CoA synthase ELOVL6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">ELOVL fatty acid elongase 6</fullName>
            <shortName evidence="14">ELOVL FA elongase 6</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Very long chain 3-ketoacyl-CoA synthase 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Very long chain 3-oxoacyl-CoA synthase 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ELOVL6</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that elongates fatty acids with 12, 14 and 16 carbons with higher activity toward C16:0 acyl-CoAs. Catalyzes the synthesis of unsaturated C16 long chain fatty acids and, to a lesser extent, C18:0 and those with low desaturation degree. May participate to the production of saturated and monounsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>dodecanoyl-CoA + malonyl-CoA + H(+) = 3-oxotetradecanoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:60140"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:62543"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:60141"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hexadecanoyl-CoA + malonyl-CoA + H(+) = 3-oxooctadecanoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:35315"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:71407"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:35316"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tetradecanoyl-CoA + malonyl-CoA + H(+) = 3-oxohexadecanoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:39167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57349"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:39168"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="14">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endoplasmic reticulum membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="14">N-Glycosylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the ELO family. ELOVL6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.199" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9P5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007210"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007302.1">
        <property type="protein sequence ID" value="ENSXCOP00000007210.1"/>
        <property type="gene ID" value="ENSXCOG00000005573.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244965"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035338">
        <property type="term" value="P:long-chain fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03206">
        <property type="entry name" value="VLCF_elongase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033675">
        <property type="entry name" value="ELOVL6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF68">
        <property type="entry name" value="ELONGATION OF VERY LONG CHAIN FATTY ACIDS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 14 15" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 14 15" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="10 14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 14 15" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 14 15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 14 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14 15">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14 15">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14 15">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14 15">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14 15">
        <location>
            <begin position="192"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14 15">
        <location>
            <begin position="230"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="265" mass="30927" checksum="7C17F0CFE032A3E1" modified="2018-12-05" version="1">MNESELNLFDFERRFDETRALEWMQENWSKSFIFCGLYAALVFGGRHFMRERPKLNLRRPLALWSLSLAIFSIIGAVRTGKYMLHIITKSGFKQSVCDNSFYNAPITKFWSYAFAISKAPELGDTAFIILRKQRLIFLHWYHHITVLLYSWFSYKDLVAGGGWFMTMNYMVHSIMYTYYAARAAGLRVPRPLAMIITTAQILQMVMGLAVLGLVYLWMNEVHCPSNMENIMWGTLMYLSYLVLFASFFYNSYVKDPSRDKGSKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LA49</accession>
    <name>A0A3B5LA49_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dilute domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LA49"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007370"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007465.1">
        <property type="protein sequence ID" value="ENSXCOP00000007370.1"/>
        <property type="gene ID" value="ENSXCOG00000005653.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159293"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051020">
        <property type="term" value="F:GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034446">
        <property type="term" value="P:substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052072">
        <property type="entry name" value="Vascular_dev_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN YPR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027:SF3">
        <property type="entry name" value="RAS-ASSOCIATING AND DILUTE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dilute" evidence="1">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="307" mass="35246" checksum="FC339698F307022C" modified="2018-12-05" version="1">MSNSIEILYFIQQRAPESLLSATITANEEAMTILEEVIMYTFQQCVYYITKALYVVLPGLLDCNPFPVDSSEPCWKGGVGFPEPVRRVLQVFQSAQELLHGYLVHPEIQAQMFAYLFFFSNVSLLNQLLDKGPARGWFQRSKSLQIQACLRMLMEWATKSGLGHLAEKFFTKLNIRLFFSLLCLQLSWRVLSSEHPSLKPVQLHRILTQYQLTAEIGPVPVWQPSIDLLESFENHPPIVLPSSGFRVDLDSECVEDSIYRQLLYVRHYLWGLRTKTQTHTTTPSVHTQSNGTNTADWPDIQVRKIHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LA80</accession>
    <name>A0A3B5LA80_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Lysosomal trafficking regulator</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000009098"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009209.1">
        <property type="protein sequence ID" value="ENSXCOP00000009098.1"/>
        <property type="gene ID" value="ENSXCOG00000006925.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156359"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF86">
        <property type="entry name" value="LYSOSOMAL-TRAFFICKING REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="3">
        <location>
            <begin position="2873"/>
            <end position="2921"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="117"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="925"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1017"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1149"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1961"/>
            <end position="2030"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="2064"/>
            <end position="2118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1186"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1961"/>
            <end position="1972"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1997"/>
            <end position="2011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="2064"/>
            <end position="2074"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="2082"/>
            <end position="2091"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="2921" mass="324487" checksum="CDEC358C3D0F8A0A" modified="2018-12-05" version="1">MSGASNTLAREFLTDVHQLCSAVAQRAEARDEDEEESHMVALGEYLVRGRGFLLLSTLDAIIDQELTCREELLTLLLSLLPLVWKIPVQEEKAPDFNLPSLLEAFLSREVKVVSLRTGQKAASDEQSSGKRSRIGSGTWKSRRSRRTSQRYSVKDARKSQVSTSDSEANAEERVVGGSRSRRPHSSTIQVSCQHHRSEASQLTSTATNTTETMSALYPHPRAPGSQMIDHETLTDPAAISIFNRMENSPFDLCHVLLSLLEKVCKFDMSINHNPGLAISVVPTLTEILTEFGDCCGLGGGGGMGAEELAGGWTEEPIALVQRMLLRTILHLMSVDISQSDRLPDSLRRSLTDLLRATLKIRSCLERQNNPFAPRSKKTLQEVQDDFSFSRYRHRALLLPELLEGVLQVLLSCLQASTPNPFFFSQVLELIHEFVQHRGLELFEATVLRLEGLSRASSDVGREASERIRGLIAGVFKIISAIKKAKSEQLHQSVCARRRHRRCEYSHFLHHHRDLSGLPISAFMQVARCNPFEEVVEDSEESEGDTVRYPERCCCLAACAHQCLRLLQRLSPSGPSVLQVLAGIQAVGICCCMDPRSVVGPLLHAFQSPGLRSYQSHVLSVLSRLILDQLGGGQPSEKAKLASCNICTLDSSQLPGLEETLQQGEPANISTSLCYRSQGILPSGGGAEDMLWKWDALEAYQELVFGEEWQLSQQIAGHVCHLTLRGNAIVQWQLYTHIFNPVLQRGVELVHHAQQLGVSTVCTQVCSYHTQCLPVEVLLIYLQTLPALLKSRVVRDLFISCNGLNQVTELVYLDQTRSLALKVFETLIIGVGHQQSDGLLHELESADTKEREAILGLAEELGSRGVGEGPQSLSKFYEGLKEVYPRHKSRTGQNPGPGRSRTETHLHVINLFLCVAFLCVSKEADSDRDSANDSEDTSGYDSTASEPLGGRLPYLSPDSVALPSKEQLRRAADVWSVCRFIYMASPLFQRQFFRLGGLDVCLRLMAMVIQKLSCKSKDGKTKKKREGKGKGSPESAVPAASLGSEDPPYESGDFHSLGPTEGKTKDPGKRFDEQWQLQSIRLLEALLAICLHSANSAMQRIEPELSSQPQSVEETLLEVRDQLSRSGVVNSDLAVPLFDSLLRVALAQVSSCTDTPEEKPERGAAPAGDLSEEVDEVQSCSVRLPGEEEGYDADSESNPETMKQQEEGWSLEANGVNGDGPERGVLLFPEICTMELRLLASGSPDLEVLGHVFHSLLGAVHANRRNATLLYDQVRLNTSSSLCWCSPSCRFDVTLHFGFSQGGMVLVELLVAMVSQRITAEELALLIRLFLEKTPPTAILLKGILQIVEANMKMEPLHFLTFPILHGVSTPGGSSPGSRQSGAAGGKAVSLLWKGKLSPGRREGEPEHRPSHLWSSPWHTAPLHLPLVGQNCWPHMAPGFSASLWLRVTELEEKSDDKGWNSLMLDLLERWTVYKISKSTEENLVHVLSMGSKALMLQVWADFSKGSLTFRICIDPNDDIKAGLLTQAESGEGLFRRGQWQHLGLTYSQQPEGKKNIHGRVVVFTLFVLYLPLSGSRIGSEEAFFLYSSGPDLTSIMPCKYGKSSGSISKFVSLEGLRCEHVREILMKNKDIDTTALVESLAIVYTPSSPRVYTIYEPVIRLKGQAKTVVTQRPFSSKEVQSISLDANAFRSLLPVETHGLQTVLHKIGGTGNFVFLFAQTVELSDCENTQALALRVLLSLCKHNQHRIHEMDCYHGYSMIHQVLIKSKCIVGYHMLKTLLDGCCSGPVLILGVDGQFRLDPESVAVVQDIRLLSDILLDWKIWAMAECGVWETLLAALEILIRIHHPHQVYNIRQFLNAEVVHRFLLTCQVLQEHRDDHLTSIPQEVSLSFIKIIQEVLGSPPDMNLLKLICNFLLAVHPPTNTYVCHTPSSFYFSLHIDGKLYQEKVQSIMYLRRSNSGGRSASSSVMSLSPTVFADGPHEGTSPSPEHGGDDQSLRPPSAAPSPYSSPLVMPCVGHGSSSDAAEGDNVSLASQQALGSTETLKKVGGDEQLLSSCESAKTICDSKDAEEGRAPRTPSISVEEERDEAAEVDSPVEGSVGGVESEDWASEEAPRRPDSLKGIQSFQRSHSNLTSLGLAFPFPNGSLAVARWPSAADRASMPEDWESYTYSPGSDRTHSKDCLVLICCGLYDLLRGVLLLLPDLMLEEVMDKLIQPEALIVLVNHPSPLIQQGVMKLLDAYFSRALKEQKEKFLKNHGFSLLANQLYIHQGSQGLLECFLEMLFGRPMGLEEDLDLEEMENISPFRRRCIIPVLGLIENSLYENSLVHNILCMLLQLVNACPKLADILLDHGLLYVLFNTLSTLNGMENGIPLNDYKLLVCDIQQMLVAVTIHSCSSSGSQYFRIIEDLITLLGFMQTSKMRRTQEMAVALQFRVLQSAIEFIKTTANQEPSKLSSAVNTPTSPHHAIYQKRKSIAGRRRFSLAQTDSLLMRMRSVASDELHQMMQRRMSQENPIRASETEFVQRLQRLVVLAVNRLIYYGKLRLILGVIPSNSHNESGTCSVPHSPTPFILPHASKKSFQKDILKLMTDGIKISLGSTGRGGAPNHQWRRILWSCRDTFRVQIGRLLVHTLSPSLPLQDRKEALEFVFDHKHIDILKESLSPGLEVNKKPSLLLDYSFEPAVGVFMTSLKLCGHRCIPLNAPHKHDLLKVIKEVKEVIVEEKMGASRVEITVTILSLIQRLDGKSKDISKIAADITQNVSLRQGMERKKVIQHIRGLYKTDLSASRHWQELVQQLTHDRAVWYDQTSYPTSWQLDPTEGPNRERRRLQRCYLTIPNKYLLKDRRKPEVKPPLSFLFEDKTHSFFSSTVKDKATSESIRFTRRCTSVAPSRETAGELLLGKSGMYFVEDNAADAHDSQVNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBC1</accession>
    <name>A0A3B5LBC1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">PR domain containing 9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBC1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005174"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005234.1">
        <property type="protein sequence ID" value="ENSXCOP00000005174.1"/>
        <property type="gene ID" value="ENSXCOG00000004050.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158211"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000340">
        <property type="entry name" value="zinc finger protein 473 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF45">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE PRDM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="10">
        <location>
            <begin position="70"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="228"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="285"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="314"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="391"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="420"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="464"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="585"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="489"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="664" mass="73576" checksum="D250577768D6DE0B" modified="2018-12-05" version="1">CSASPEPEPNEEKQLSASMHHKFGTNFQKTECLPLFQDQSNSDDINGPSFILDFPTTMGVPQRALLTLPFGLMIGRSSIPSAGVGVINQGLALSPGMHFGPYEGEVTVKENAITSDFSWEIYKGEDQYEYIDATSDSYSNWMRYVNFARSKDEANLLAVQYKGSILFHCCRAVQTGDELTVWPSSKLRAHLSKDWSDMLSLKMSRTGSFHFPNRSPKSSGESASSPIFLCSNCLLSFTTESFLLRHIEHFHSQPKADEAAGEENPGSRDEPPASLVMLVDPVKSKTCDDCGKVFKQVPHLKRHKLCVHSNKRPYCCSHCRRSFSQASGLVRHQSVHFGRAAATDKPVSTAESSALGSELSNWAEAASSEETERSNPTVVAQVDVYVETQQLDCSVCGKSFTNEAHLKKHEAAVHEKLRPYVCSVCLKCFGQYNDLARHLQSHQKKNEHKEKMDEDSEDAATMPFSCGECAVAFSSVDDLQLHINELHSEESTAETQGSSSAPPVETKDLTLNSAPLRPQRLKARSKVSAVTQLVAPKRKAPNQQEDSVARNAKIKKYKWFSCNLCKQTYGNPDDLKAHKCTSKPLNCGQCSAAFWKSSLLRRHKQMVHGRVKSFCCDSCDKAFSTSAKLRHVFPCATRGGCQVQLLVRDENFSAGNDFGKGQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDY2</accession>
    <name>A0A3B5LDY2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP synthase peripheral stalk subunit F6, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">ATP synthase peripheral stalk subunit F6</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Subunit F6, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the ATP synthase complex composed at least of ATP5F1A/subunit alpha, ATP5F1B/subunit beta, ATP5MC1/subunit c (homooctomer), MT-ATP6/subunit a, MT-ATP8/subunit 8, ATP5ME/subunit e, ATP5MF/subunit f, ATP5MG/subunit g, ATP5MK/subunit k, ATP5MJ/subunit j, ATP5F1C/subunit gamma, ATP5F1D/subunit delta, ATP5F1E/subunit epsilon, ATP5PF/subunit F6, ATP5PB/subunit b, ATP5PD/subunit d, ATP5PO/subunit OSCP. ATP synthase complex consists of a soluble F(1) head domain (subunits alpha(3) and beta(3)) - the catalytic core - and a membrane F(0) domain - the membrane proton channel (subunits c, a, 8, e, f, g, k and j). These two domains are linked by a central stalk (subunits gamma, delta, and epsilon) rotating inside the F1 region and a stationary peripheral stalk (subunits F6, b, d, and OSCP).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ATPase subunit F6 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDY2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008785"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008891.1">
        <property type="protein sequence ID" value="ENSXCOP00000008785.1"/>
        <property type="gene ID" value="ENSXCOG00000006707.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008902"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.110:FF:000001">
        <property type="entry name" value="ATP synthase-coupling factor 6, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.110">
        <property type="entry name" value="Mitochondrial ATP synthase-coupling factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008387">
        <property type="entry name" value="ATP_synth_f6_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036204">
        <property type="entry name" value="ATP_synth_f6_sf_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12441">
        <property type="entry name" value="ATP SYNTHASE COUPLING FACTOR 6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12441:SF14">
        <property type="entry name" value="ATP SYNTHASE-COUPLING FACTOR 6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05511">
        <property type="entry name" value="ATP-synt_F6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111357">
        <property type="entry name" value="Mitochondrial ATP synthase coupling factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="85" mass="9398" checksum="0043A4362F05133A" modified="2018-12-05" version="1">MDPVQRLFLDSIREYSTNSQAAGGLVDAGSQYQKVLEEEIAKLQRLYGGGDLTSFPNFKFTGKLTIGYLVFSTDGKFLTIPVKIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEP3</accession>
    <name>A0A3B5LEP3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SUMO-activating enzyme subunit 2</fullName>
            <ecNumber evidence="12">2.3.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterodimer of SAE1 and UBA2/SAE2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1. Interacts with UBE2I.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.-" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LEP3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008156"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008254.1">
        <property type="protein sequence ID" value="ENSXCOP00000008156.1"/>
        <property type="gene ID" value="ENSXCOG00000006258.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074924"/>
    <dbReference type="UniPathway" id="UPA00886"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033334">
        <property type="term" value="P:fin morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061035">
        <property type="term" value="P:regulation of cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061074">
        <property type="term" value="P:regulation of neural retina development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01489">
        <property type="entry name" value="Uba2_SUMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.520:FF:000002">
        <property type="entry name" value="SUMO-activating enzyme subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.20:FF:000002">
        <property type="entry name" value="SUMO-activating enzyme subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000002">
        <property type="entry name" value="SUMO-activating enzyme subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.520">
        <property type="entry name" value="Ubiquitin activating enzymes (Uba3). Chain: B, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.80">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.20">
        <property type="entry name" value="Ubiquitin-like 2 activating enzyme e1b. Chain: B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028077">
        <property type="entry name" value="UAE_UbL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042449">
        <property type="entry name" value="Ub-E1_IAD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023318">
        <property type="entry name" value="Ub_act_enz_dom_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030661">
        <property type="entry name" value="Uba2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032426">
        <property type="entry name" value="UBA2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF5">
        <property type="entry name" value="SUMO-ACTIVATING ENZYME SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14732">
        <property type="entry name" value="UAE_UbL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16195">
        <property type="entry name" value="UBA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039133">
        <property type="entry name" value="SUMO_E1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8 12 15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="18">
        <location>
            <begin position="1"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin/SUMO-activating enzyme ubiquitin-like" evidence="19">
        <location>
            <begin position="394"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="SUMO-activating enzyme subunit 2 C-terminal" evidence="20">
        <location>
            <begin position="491"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="156"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="491"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="501"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="526"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="541"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="582"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="13 16">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="2"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="10"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="49"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="71"/>
            <end position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR039133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR039133-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR039133-2"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR039133-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF14732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF16195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="593" mass="65833" checksum="2DCE9E5C4941C5C7" modified="2018-12-05" version="1">IDLDTIDVSNLNRQFLFQKKHVGKSKAQVAQESALQFCPSANITAYHDSIMNPDYNVEFFRKFMLVMNALDNRAARNHVNRMCLAADIPLIESGTAGYLGQVTVIKKGMTECYECQPKPAQKTFPGCTIRNTPSEPIHCIVWAKYLFNQLFGEEDADQEVSPDTADPEAAWNPEDTAARATASEQDGDIRRVSTKEWARSTGYDPVKLFNKLFKDDIMYLLTMDKLWKKRKAPAPLDWQQLENSATSGLKDQQVLDVWGYCRLFRHSVETLRAQLQEKGEGAELVWDKDDPPAMDFVTAAANLRMNIFSMNMKSRFDVKSMAGNIIPAIATTNAVIAGLIVLKGLKILSGELESCRTIFLNKCPNLRKKLLVPCILDKPSPNCYVCASKPEVTVKVNVQKTTVLCLQDKILKERFGMVAPDVQIEDGKGTILISSEEGETETNNNKFLSDFGIRNGSRLQSDDFLQDYTLLINVLHTEELERDVEFEVVGEAPDKAPPPQTNQEEVNSITNGNKDSAQPSTSSKADDDDIMIVDSDEEVGAPSSSAAPPSATKRKHQEDSETGETSTKRPRTDQSAAATAADNDDDDDIIALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LG67</accession>
    <name>A0A3B5LG67_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">COMM domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">COMMD3</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the COMM domain-containing protein 3 family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000008671"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008777.1">
        <property type="protein sequence ID" value="ENSXCOP00000008671.1"/>
        <property type="gene ID" value="ENSXCOG00000006627.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015971"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017920">
        <property type="entry name" value="COMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037355">
        <property type="entry name" value="COMMD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31159">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31159:SF1">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07258">
        <property type="entry name" value="COMM_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21672">
        <property type="entry name" value="COMM_HN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51269">
        <property type="entry name" value="COMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COMM" evidence="3">
        <location>
            <begin position="110"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00093469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="333" mass="37269" checksum="8B0853F7A4A8C775" modified="2018-12-05" version="1">MELSESVQKGLQLLADQSAVNHRSFQVLLDVSSRGLLSSRADPTVLDQPELKHMDRILLKQSHAALTTFILEAVKHNADKSTTSSCLEELTFSTDRIEIFFNAYQKHKNDIERLLSRTKVDGFALYFPKPTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPPPPQQNGQLDKINEPFYLISLNTENEGSSEDINFSCTTEQLQDLVSKLKDAAKSVEKASQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LH21</accession>
    <name>A0A3B5LH21_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ena/VASP-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Ena/vasodilator-stimulated phosphoprotein-like</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Stress fiber</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Ena/VASP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LH21"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009890"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010008.1">
        <property type="protein sequence ID" value="ENSXCOP00000009890.1"/>
        <property type="gene ID" value="ENSXCOG00000007496.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157826"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005522">
        <property type="term" value="F:profilin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030838">
        <property type="term" value="P:positive regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01207">
        <property type="entry name" value="EVH1_Ena_VASP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22185">
        <property type="entry name" value="WH2_hVASP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000071">
        <property type="entry name" value="Enah/Vasp-like, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038023">
        <property type="entry name" value="VASP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014885">
        <property type="entry name" value="VASP_tetra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202:SF4">
        <property type="entry name" value="ENA_VASP-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08776">
        <property type="entry name" value="VASP_tetra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118370">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein, VASP, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0729">SH3-binding</keyword>
    <feature type="domain" description="WH1" evidence="12">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="139"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="177"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="247"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="340" mass="36767" checksum="DD572E0EAD4F4124" modified="2018-12-05" version="1">IEQSICQARASVMVYDDTSKKWVPIKPGQQGFSRINIYHNTASNTFRVVGVKLQDQQVVINYSIVKGLKYNQATQTFHQWRDARQVYGLNFASKEEATTFSNAMLFALNVLSAQDGGPALQRQVQNGPTSDEIDAQRARQMMEQQQQMQAHMERERRSSNSGSPFQGHPAVLSVAPPVVPPPLNMGGPPPPPPPPGPPPPSAAAPPLPPPLPIGGGSQGDEGPAPTGLAAMIAGAKLRRVQRPEDGSSGTKNEANRTSGGSGGLMEEMNALLARRRKAASEKPDDSQNVNGARKPWDRANSPGKLISHEEILDEVFRELHKVKDEIIDAIRNELSGVSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LH69</accession>
    <name>A0A3B5LH69_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">YY1 transcription factor a</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">YY1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YY transcription factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LH69"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009935"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010053.1">
        <property type="protein sequence ID" value="ENSXCOP00000009935.1"/>
        <property type="gene ID" value="ENSXCOG00000007537.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154763"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000104">
        <property type="entry name" value="Transcriptional repressor protein YY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000109">
        <property type="entry name" value="Transcriptional repressor protein YY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000163">
        <property type="entry name" value="transcriptional repressor protein YY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000174">
        <property type="entry name" value="Transcriptional repressor protein YY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017114">
        <property type="entry name" value="YY1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF19">
        <property type="entry name" value="YY2 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037113">
        <property type="entry name" value="TF_Yin_yang"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="272"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="300"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="330"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="118"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="361" mass="40193" checksum="3C2BF73E21256847" modified="2018-12-05" version="1">MASGDTLYIETDGSEMPAEIVELHEIEVETIETTVVGEDGEQQPMIALQPLDDPHHLHSHQEVILVQTREEVVGEEDSELHTEDGYEDQILIPVPAVEEDYIEHTLVTVAGRSTSAGRVKKSGSGKKAGKKSYMSGGEMGRKWEQKQVQIKTLEGEFSVTMWASDDKKDIHEEQIAGENSPPDYSEYMTGKKLPPGGIPGIDLSDPKQLAEFARGFEANMVKAAPSALMKPRKVKEDDAPRTIACPHKGCTKMFRDNSAMRKHLHTHGPRVHVCAECGKAFVESSKLKRHQLVHTGEKPFQCTFEGCGKRFSLDFNLRTHVRIHTGDRPYVCPFDGCNKKFAQSTNLKSHILTHAKAKNNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LH88</accession>
    <name>A0A3B5LH88_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LH88"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007234"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007326.1">
        <property type="protein sequence ID" value="ENSXCOP00000007234.1"/>
        <property type="gene ID" value="ENSXCOG00000005593.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166669"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001221">
        <property type="term" value="F:transcription coregulator binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051638">
        <property type="entry name" value="CTBP_dehydrogenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46029">
        <property type="entry name" value="C-TERMINAL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46029:SF3">
        <property type="entry name" value="C-TERMINAL-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="523"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="567" mass="60789" checksum="0869E8FE6511668E" modified="2018-12-05" version="1">MIGGTSVPAQDYYLADASLSGQPPEESGFYRDNQHSLTRSASHYGGVRPAWDQGQARATPSILASASAPTSVPPPPLPPPPPLPPPPLHELSRLYREALGSKMIPDVQRIGGSSFTPAAIYGVQPPLPIYASEPPSLPAHQIYNSITSLPLEPRQPTGIGSVVDPVSQVMDGVLPRAYGALASQRLPYDPNYDPASVMVAATAGMPSNLPPVHPSAADPKKMVDPTFLAFLRAEGLAESTITLLLQHGFDSTATLTMMEDHDVRSVAPNLAQARVLSRLVMGCKTSGAATRTRSNSFSHRNDLYMQPQGLTMDTSLMQQPSTTIQTVSPRMGEFLGRRPSSAPSQHLLETTTYPGARPLATGAFPVSPGGYNNVVPQARPLSMYNPHTGLAMSALGQQPPPAPGTPGTAPKTFSGSYSPMELMKRAPNLPPASPIAAASPLHSPQLLRKGISAALESTIVPSTSNTTLQGQTTTNNKLVGRRTGPPVIVSTMTTAPDTNYNRHCLFLFASFKIKFKPLFNKKICLILELTFSVIISSYFLLFFSNQYIKYLLCVFISQLIASHQLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHV6</accession>
    <name>A0A3B5LHV6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Small ribosomal subunit protein mS27</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">28S ribosomal protein S27, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Mitochondrial ribosomal protein S27</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the mitochondrion-specific ribosomal protein mS27 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHV6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010190"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010309.1">
        <property type="protein sequence ID" value="ENSXCOP00000010190.1"/>
        <property type="gene ID" value="ENSXCOG00000007727.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007246"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000232">
        <property type="entry name" value="28S ribosomal protein S27, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034913">
        <property type="entry name" value="mS27/PTCD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019266">
        <property type="entry name" value="Ribosomal_mS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21393">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21393:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10037">
        <property type="entry name" value="MRP-S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0820">tRNA-binding</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="18">
        <location>
            <begin position="84"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00074987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00075386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="204" mass="23143" checksum="31223392A4925540" modified="2018-12-05" version="1">MDRTYDRNLPVSSLTISRFVDNISSREEVDQAEYYLYKFRHSPNCWYLRDWTVHTWIRQCLKYGARDKALHTLKNKTQYGIFPDDFTFNLLLDSYIKDGDFKSACSVVEELMLQEAFDLPSTQILSLNALGSFLATKPQLSVSEERALGASLLICGLKQDNTVGFSAQLLGNALLGVYLTVFFSLFCLQFFIGAVIGLCITLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LI82</accession>
    <name>A0A3B5LI82_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPII-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SEC23/SEC24 family. SEC24 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LI82"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007569"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007665.1">
        <property type="protein sequence ID" value="ENSXCOP00000007569.1"/>
        <property type="gene ID" value="ENSXCOG00000005838.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182924"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01479">
        <property type="entry name" value="Sec24-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000020">
        <property type="entry name" value="protein transport protein Sec24D isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.380:FF:000003">
        <property type="entry name" value="SEC24 homolog D, COPII coat complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041742">
        <property type="entry name" value="Sec24-like_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF5">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC24C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="10">
        <location>
            <begin position="391"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="11">
        <location>
            <begin position="468"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="13">
        <location>
            <begin position="717"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="12">
        <location>
            <begin position="813"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="9">
        <location>
            <begin position="931"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="90"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="180"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="206"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="260"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1061" mass="115657" checksum="F904AA9BA3443A8B" modified="2018-12-05" version="1">QCLCVSLPSAPPHSSQPPISTYSAPPQPSYTQAPPPASSAPTQPPPPGVPAASQQYYGVPPLPSQQPFDSSRPPTSQQQFTSSAPPPPSQQTFPPSSFSGPPTRPQAVPVSQPQPPFSAALPSLSSAPPPVSQPGFAGGPPPIAQGSFPPQLTPPSSQPGSFPPPGPPPTSVASGQYPPMSQPQQPPAAQPSPYHSGPPPPRAQMPPTSMGQSNHLPPGPQGPLGQQGPPGPLQQPPVQPGMQTGYPPQQNGAFGQARSPQPGYAGPYPGQPNYGAPAPAPTAAPAPQKRLDPDAIPSPIQVIEDDKAKSNEPFTTGLRGQAPPLVTTNFQVKDQGNASPRFIRCTAYNMPCTSDMAKQSQVPLAAVIKPLATLPPDEAPPYVVDHGETGPIRCNRCKAYMCPYMQFIEGGRRFRCGFCSCVTEVPPHYFQHLDHTGKRVDCYDRPELSLGSYEYLATVDYCKNNKLPQPPAFIFLIDVSYNAVKSGMVSIICNELKSLLDLLPRYPNPVDSVIRVGFVTYNKVLHFYNVKSTLAQPQMLVVSDVSDMFVPLLDGFLVNVNESRQVIESLLDQIPEMFADTRETETVFGPVIQAGLEALKAADCAGKLFVFHTSLPIAEAPGKLKNREDKKLIGTDKEKTLFQPQVGVYNNLAKECVAQGCCVDLFLFPNQYVDVATLGVVPVSTGGSIYKYTYFQAQTDQERFLNDLRRDVQKQVGFDAVMRVRTSTGIRATDFFGSFYMNNTTDVELAGLDCDKAITVEFKHDDKLSEETGALMQCAVLYTSCSGQRRLRIHNMAVNCCAQLADLYRNCETDTIINYFAKYAFRGVANNPTKAVRDTLVNQCAQILACYRKNCASPSSAGQLILPECMKLLPVYLNCMMKSDVLLPGADVSLDDRAYLKQLIGCMDVAETHVFFYPRLLPVMKLESGSLPVAVRDSEERLSKGGVYLMETGLHLFLWVGASVQQELLLNIFGTPSFSQIDPNMISLPVLDNPFSQRLRELVDSFRAQRSRYMKLMVVKQEDRAELTFRHFLVEDKSASGGASYVDFLCHMHKEIRQLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIW1</accession>
    <name>A0A3B5LIW1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">small monomeric GTPase</fullName>
            <ecNumber evidence="5">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Phagosome membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="5"/>
    <dbReference type="RefSeq" id="XP_027864494.1">
        <property type="nucleotide sequence ID" value="XM_028008693.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027864496.1">
        <property type="nucleotide sequence ID" value="XM_028008695.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027864497.1">
        <property type="nucleotide sequence ID" value="XM_028008696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIW1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012018"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012156.1">
        <property type="protein sequence ID" value="ENSXCOP00000012018.1"/>
        <property type="gene ID" value="ENSXCOG00000009094.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114139061"/>
    <dbReference type="KEGG" id="xco:114139061"/>
    <dbReference type="CTD" id="51209"/>
    <dbReference type="GeneTree" id="ENSGT00940000160481"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04116">
        <property type="entry name" value="Rab9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000360">
        <property type="entry name" value="RAB9B, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041824">
        <property type="entry name" value="Rab9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF17">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB-9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="202" mass="22689" checksum="281B40DFB6B4FA4C" modified="2018-12-05" version="1">MSRNNVLLKVILLGDGGVGKSSLMNRYITDRFDSQSFHTIGVEFLNRDLEVDGRPVTLQIWDTAGQERFKSLRTPFYRGADCCLLTFSVNDLQSFLNLSCWKKEFMFYSDVKEPESFPFVVLGNKVDMEQREVGEDEARAWCEENGCCPYFETSAKDDTNVTAAFEAAVRVVLAAEDQIGHALLGSTIDLHGNRKTSRGSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJK1</accession>
    <name>A0A3B5LJK1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fibroblast growth factor</fullName>
            <shortName evidence="3">FGF</shortName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027882649.1">
        <property type="nucleotide sequence ID" value="XM_028026848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJK1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012263"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012409.1">
        <property type="protein sequence ID" value="ENSXCOP00000012263.1"/>
        <property type="gene ID" value="ENSXCOG00000009280.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114150449"/>
    <dbReference type="KEGG" id="xco:114150449"/>
    <dbReference type="CTD" id="27006"/>
    <dbReference type="GeneTree" id="ENSGT00940000161721"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033278">
        <property type="term" value="P:cell proliferation in midbrain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021872">
        <property type="term" value="P:forebrain generation of neurons"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042063">
        <property type="term" value="P:gliogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030917">
        <property type="term" value="P:midbrain-hindbrain boundary development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021509">
        <property type="term" value="P:roof plate formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd23321">
        <property type="entry name" value="beta-trefoil_FGF22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000004">
        <property type="entry name" value="Fibroblast growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00263">
        <property type="entry name" value="HBGFFGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0339">Growth factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="195"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="198"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="221" mass="24399" checksum="6EC045150D4AF5BE" modified="2018-12-05" version="1">MCRWMTSAAHLCPELTLRAATTASFTGTLIPQPSASSFALVCLTLLLVTAGSGACPPRAGHESLQGLGSGTNCSWTLERHTRSYNHLEGDVRLRRLYSANKFFLCIDKTGKVDGTRRKNYADSLMEIRSVSVGVVAIKSVNTGLYLAMSKKGTLFGSVTYSPSCKFKERIEENGYNTYASLRWKHGGRQMFVSLNGRGKPRRGHKARRRHPSTHFLPMLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJW1</accession>
    <name>A0A3B5LJW1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJW1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010935"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011060.1">
        <property type="protein sequence ID" value="ENSXCOP00000010935.1"/>
        <property type="gene ID" value="ENSXCOG00000008260.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271963"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046427">
        <property type="term" value="P:positive regulation of receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003531">
        <property type="entry name" value="Hempt_rcpt_S_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF27">
        <property type="entry name" value="INTERLEUKIN-7 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01355">
        <property type="entry name" value="HEMATOPO_REC_S_F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="10">
        <location>
            <begin position="81"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="313"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="356" mass="39454" checksum="4CC93F58D34E5464" modified="2018-12-05" version="1">SANNLTCQVLGRHGDEDDEDGDRDAIDKMINDRTVEKCVRTHGDTVSGLCPVTIQKVHFNFQVKLAKVLMFQNLLLCVPVKPRSPWIVNVTMDPVMNRAVIYFQIPYRKDYLNLNTQSFQLNLWTDGSNMTQNVSSKDSLQIDRQHLRQDSEYRVRVRSIPVGVLQGTWSAWSPEFSFHTPPASDIISCPSAEEKPPSPDESERTVSQLVLSSVSVLVVLCCIVVFWKTGIFSYMWPSIPHPKQALVQICKPNKGLLLDLHHEVFSSLRVLPLEKLQAEELGSGSGSSHSSASSAGTEELEVSALLSCEDGCVPGGAPSPVDVPRPEDKSQNLLGRRGGPGQEEAYVTMSSFYQNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKC1</accession>
    <name>A0A3B5LKC1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKC1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011100"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011227.1">
        <property type="protein sequence ID" value="ENSXCOP00000011100.1"/>
        <property type="gene ID" value="ENSXCOG00000008389.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000270661"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="118" mass="14385" checksum="98473898A1B90A30" modified="2018-12-05" version="1">MHITLFLFLFVKKKKRREREKSHLSFSFHPKLTPYYCVELIYHMRDPIKYIEVCGFNVRGSSAKHCRYIILLDPQSGPRRRRSWLWNQFFVIEEYRGPEPVLIGRVFILLELFDINKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKQ0</accession>
    <name>A0A3B5LKQ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7 9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKQ0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011245"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011376.1">
        <property type="protein sequence ID" value="ENSXCOP00000011245.1"/>
        <property type="gene ID" value="ENSXCOG00000008496.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012063"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019258">
        <property type="entry name" value="Mediator_Med4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13208">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13208:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10018">
        <property type="entry name" value="Med4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="212"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="78"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="244"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="255" mass="28512" checksum="622363CA49840BF7" modified="2018-12-05" version="1">MAGRRRRIKSTKERLLSVLDDLEVLSRELIEMLALSRSQKLPQPGEDTQILELLVQRDKEFQELMEVAQQQGKVHQEMQLLEKEVEKRDSDIQQLQKQLKEAEHILATAVYQAKEKLKSIEKARKGSISSEEIIKYAHRISASNAVCAPLNWVPGDPRRPYPTDLEMRSGMLGHMANLPSNGMNGHLPGDALAAGRLPDVLTPHYPWQPSDVSVGMLPPHHGNDFGLEPPGHNKENEDDVEAMSTDSSSSSSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKX3</accession>
    <name>A0A3B5LKX3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Stathmin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the stathmin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKX3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012733"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012887.1">
        <property type="protein sequence ID" value="ENSXCOP00000012733.1"/>
        <property type="gene ID" value="ENSXCOG00000009623.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234597"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031110">
        <property type="term" value="P:regulation of microtubule polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030514">
        <property type="entry name" value="Stathmin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000956">
        <property type="entry name" value="Stathmin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036002">
        <property type="entry name" value="Stathmin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10104">
        <property type="entry name" value="STATHMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10104:SF21">
        <property type="entry name" value="STATHMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00836">
        <property type="entry name" value="Stathmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002285">
        <property type="entry name" value="Stathmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00345">
        <property type="entry name" value="STATHMIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101494">
        <property type="entry name" value="Stathmin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01041">
        <property type="entry name" value="STATHMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51663">
        <property type="entry name" value="STATHMIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="123"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="156"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="172" mass="19927" checksum="CC002CB6BF6C9E0A" modified="2018-12-05" version="1">MNPHILLLTLEKMRELPLVSIFCSCILPQPREQTANKQEGVVDLNLCNIRDMEVIELSKRASGQAFEVILKPPTFDGAPELRATTPPRQKPSLAEIQKKLDAAQERRKCQEAELLKHLAERREHEREVAQKALTKDRQEQRVNAKPLQMHLNATQEEDKHSVEVRMRMRRQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LL30</accession>
    <name>A0A3B5LL30_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LL30"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012952.1">
        <property type="protein sequence ID" value="ENSXCOP00000012798.1"/>
        <property type="gene ID" value="ENSXCOG00000009674.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286981"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="MDS1 and EVI1 complex locus"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF44">
        <property type="entry name" value="ZINC FINGER PROTEIN 271-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="9"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="65"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="113" mass="13219" checksum="720A005FEA86925F" modified="2018-12-05" version="1">RRRMPPKPFTCHICGARFNLNNNLKRHLRIHTGEKPFTCKDCGKSFSDNNKLKSHMLVHGARKPFMCDLCGKTFLFNCRLQMHQRYVHADNSHASDAAQTEIFIPLLLKKKFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LL31</accession>
    <name>A0A3B5LL31_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">N-ethylmaleimide-sensitive factor attachment protein, alpha b</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SNAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LL31"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011380"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011512.1">
        <property type="protein sequence ID" value="ENSXCOP00000011380.1"/>
        <property type="gene ID" value="ENSXCOG00000008598.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005826"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070044">
        <property type="term" value="C:synaptobrevin 2-SNAP-25-syntaxin-1a complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005483">
        <property type="term" value="F:soluble NSF attachment protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010807">
        <property type="term" value="P:regulation of synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035249">
        <property type="term" value="P:synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15832">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000028">
        <property type="entry name" value="beta-soluble NSF attachment protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000744">
        <property type="entry name" value="NSF_attach"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768:SF23">
        <property type="entry name" value="ALPHA-SOLUBLE NSF ATTACHMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13768">
        <property type="entry name" value="SOLUBLE NSF ATTACHMENT PROTEIN SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14938">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00448">
        <property type="entry name" value="NSFATTACHMNT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="278" mass="31376" checksum="1BC13A4242EFB32E" modified="2018-12-05" version="1">MDNSGKEKEAAALMAEAEKKLKSSQSFFGSMFGGSSKLEEACDMYVRAANMYKMAKNWCAAGNAFSKAAHLHLQMQSKHDAATNLIDAGNAFKKADPQEAINCLNRAIEIYTDMGRFTIAAKHHMSIAEIYETELVDIDKAIAHFEQAADYYKGEESTSSANKCLLKVATYAAQLEQYQKAIEIYEQVGTHAMDSTLLKYSAKDHFFKAALCHFCVDMLNAKLAVQKYEEMFPAFSDSRECKLLKVKEYDTISRLDQWLTTMLLRIKKTIQDDENDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLT5</accession>
    <name>A0A3B5LLT5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Solute carrier family 40 member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May be involved in iron transport and iron homeostasis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ferroportin (FP) (TC 2.A.100) family. SLC40A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLT5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011912"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012048.1">
        <property type="protein sequence ID" value="ENSXCOP00000011912.1"/>
        <property type="gene ID" value="ENSXCOG00000009016.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17480">
        <property type="entry name" value="MFS_SLC40A1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009716">
        <property type="entry name" value="Ferroportin-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11660">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 40 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11660:SF47">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 40 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06963">
        <property type="entry name" value="FPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="57"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="309"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="341"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="248"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="257"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="271"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="569" mass="61529" checksum="58127BF03C88A162" modified="2018-12-05" version="1">NNYNNNTNNNISSESIRHFFTSAKFLIYMGHALSTWGDRMWNFAVAVFLVELYGNSLLLTAVYGLVVAGSVLLLGANIGNWVDRNPRLKVAQTSLLVQNSCVIMCGVLLMLVFHFKAQLVELYNGWILTVCYILVISIANVANLASTATSITIQRDWVVVVAGQDSSSLADMNATVRIIDQLTNILAPMLVGQIMAFGSHFVGCGFISGWNLASMCVEYGLLWKVYQKTPALALKAVHKEQQHELKQLSPLKGTDCENGQSPEESSQPLMDASPPGAADPGKAAGCCGAASQPLRTFRAGWVAYYKQDIFLAGMSLAFLYMTVLGFDCITTGFAYTQGLNGSILSLLMGASAISGICGTVAFTWIRRKCGLIRTGFISGTAQLCCLLLCVFSVFAPGSPFDLSVSPFQDLYAHLMGQQSLPEAEHSLGAAFSTNATTPPPLAQEPPPLQSYMSVSLLFAGVIAARVGLWSFDLTVTQLIQENVIESERGVINGVQNSMNYLLDLLHFIMVILAPNPEAFGLLVIISVSFVAMGHAMYFRFAFKSLGSRLFLCCSPEQKAETDASPPTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMW8</accession>
    <name>A0A3B5LMW8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Netrin receptor UNC5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the unc-5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMW8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012055"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012193.1">
        <property type="protein sequence ID" value="ENSXCOP00000012055.1"/>
        <property type="gene ID" value="ENSXCOG00000009124.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182815"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005042">
        <property type="term" value="F:netrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033564">
        <property type="term" value="P:anterior/posterior axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000001">
        <property type="entry name" value="Unc-5 netrin receptor B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000002">
        <property type="entry name" value="Unc-5 netrin receptor C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.30:FF:000003">
        <property type="entry name" value="Unc-5 netrin receptor C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000037">
        <property type="entry name" value="Unc-5 netrin receptor C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000039">
        <property type="entry name" value="Unc-5 netrin receptor C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037936">
        <property type="entry name" value="UNC5A-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057755">
        <property type="entry name" value="UNC5A-D-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033772">
        <property type="entry name" value="UPA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582">
        <property type="entry name" value="NETRIN RECEPTOR UNC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12582:SF4">
        <property type="entry name" value="NETRIN RECEPTOR UNC5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25609">
        <property type="entry name" value="Unc5_NetrinR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17217">
        <property type="entry name" value="UPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00218">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 13" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="281"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="134"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="ZU5" evidence="15">
        <location>
            <begin position="418"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="819" mass="91846" checksum="3A59E3F6432A81DB" modified="2018-12-05" version="1">WHLLHKHPATAALPAAFSDLLPHFLVEPEDEYIVKNKPVTLSCRSTPATQIYFKCNGEWVHQDDHLIERTVDPATALPVMQVTIEITRQQVEKIFGLDEYWCQCVAWSTTGTQKSQKAYIRIAYLRKNFEEEPQGKEVALDQEVLLHCHPPEGVPQAEVEWQRNEDVLDPLSDPNFFITPDNNLVIKKARLLDTGNYTCVAKNIVARRKSNSATVLVYVDGGWSEWSKWSACGADCSMWRSRECSQPSPGTGGKDCQGLDLQSMNCTNEQCPQTVSGAEDVALYVGMVAVALCLTLLLIVVVLVYRRKKEGLDADVADSSILTTGFQPMGIKPSKPENSHLLTIQPDLTTTTTSYQGTLRSRHDTAGKFSMTNGPLLDPLPNGSHTLHNGKLNSDTTEFVSRLSTQTYFKTLPRDVANTSYGTFNFLGGRLTIPNTGISLLIPPEAIPRGKIYEIYLTVQRKEDLRLPLAGCQTLLSPIVSCGPPGVVLSRPVILSMDHCFDACLDNWAVRLKKQNYEGAWEDVLLMGEELVSEPYYCQLEAETCRLFTEQLGTFALVGESLHMGAAKRLRLVLFSDAYCSTLEYNIRVYCMDDTQDVFKEVMQMERQLGGRLIDEPHVLLFKDSYHNLRLSIHDMPQAHWRSKLLAGYQEIPFYHIWNGSQRYLHCTFTLERLSLSTCELTCQLCVWQVEGEGQSFALDFNIAKDTRGLDSEFLLMDSNATALAGPSAFQIPYLIRQKICGSLDAPCPNGADWRMLAQRLKLERHMSFFASKASPTSVILDLWEAQHFHSGNINQLATVMADIGKQEAMIFLVSDGEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQ17</accession>
    <name>A0A3B5LQ17_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IF rod domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027890826.1">
        <property type="nucleotide sequence ID" value="XM_028035025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQ17"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012870"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013027.1">
        <property type="protein sequence ID" value="ENSXCOP00000012870.1"/>
        <property type="gene ID" value="ENSXCOG00000009735.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114155237"/>
    <dbReference type="KEGG" id="xco:114155237"/>
    <dbReference type="CTD" id="767716"/>
    <dbReference type="GeneTree" id="ENSGT00950000182969"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF354">
        <property type="entry name" value="KERATIN, TYPE I CYTOSKELETAL 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="5">
        <location>
            <begin position="72"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="76"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="267"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="428" mass="48494" checksum="4AC0B3390654B6B5" modified="2018-12-05" version="1">MASTLSVRSLSVHQPSFSSVTVRDSGRSLRSKPPVSSLSTVSTLSLSRSVSMGNGLNMLSSSLSLNGLGASEKETMQGLNDRLANYLDKVRSLERSNAELEVKIKHLMMERTPKGHDIEGMMAQAHTIGQEMRKRTLENARIMLEIDNAKLAADDFRVKWEAEASLCQSVERDCQALRRAKSDHDQIIATLRGDLDSLKEEMYYLKKNHDEEKSTLKARLATEQVSVEVEAAQGPDLGAIMAELRVQYEGIARKNKEDAEAWYLKKLEAVQCEVKESNEALRCAQGELSERRRFLQALEVELDNLRKQVSVLEGNLGETGHKYTVEMDRLQATLTQLEDELSQLRLDMQRNKTDYEQLLRIKQNLEMEIATYRRLLEGEEMVKETLPPPKKDPEVRTRKIVKVVTQTMINGKVVEESSEVEQIEDSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQ25</accession>
    <name>A0A3B5LQ25_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane protein 184C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TMEM184C</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQ25"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009884"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010002.1">
        <property type="protein sequence ID" value="ENSXCOP00000009884.1"/>
        <property type="gene ID" value="ENSXCOG00000007497.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155201"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="383"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="383"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="437" mass="49470" checksum="96F3259B5A437402" modified="2018-12-05" version="1">MPCTCAEWRRWIRPLVLVLYALLLVAVVPLCVWELLKDKVGTHSKAWFIAGVFVFLTIPVSLWGILQHMVHYTQPELQKPIIRILWMVPIYSLDSWLALRYPSLAIYVDTCRECYEAFVIYNFLVFLLNFLSNQYPSLVLMLEVQQQQPHLPPLCCCPPWPMGEVLLFRCKLGVLQYTVVRPVTTVIALICQLCGVYDEANFSFRNAWSYLVIVNNISQLFAMYCLVLLYKALRDELTPIRPVGKFLCVKLVVFVSFWQAVFIAFLVKVGVISDKHTWDWDSVEAVATGLQDFIICIEMFLAAIAHHYTFTYKPYVQEAEEGSCFDSFLAMWDFSDIRADVTEQVRHVGRTFLGRPNKMYFGAAARPEHTEHTGLLNSTSQDPVVAAATSMPSSPSSVGRYQGLGHTTAPHSISAPAGFTASSWEDDSDGTPPQPGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQB2</accession>
    <name>A0A3B5LQB2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Actin-related protein 2/3 complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Arp2/3 complex plays a critical role in the control of cell morphogenesis via the modulation of cell polarity development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ARPC5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQB2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011606"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011738.1">
        <property type="protein sequence ID" value="ENSXCOP00000011606.1"/>
        <property type="gene ID" value="ENSXCOG00000008760.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154654"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.190:FF:000001">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.190">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006789">
        <property type="entry name" value="ARPC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036743">
        <property type="entry name" value="ARPC5_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12644">
        <property type="entry name" value="ARP2/3 COMPLEX 16 KD SUBUNIT P16-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04699">
        <property type="entry name" value="P16-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039096">
        <property type="entry name" value="p16-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69103">
        <property type="entry name" value="Arp2/3 complex 16 kDa subunit ARPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="155" mass="17418" checksum="3D6485D795578C15" modified="2018-12-05" version="1">MSKNTVSARFRRVDVDEYDENKFVDEEDGGENQLGPDEAEVDSFIRQYPLYYKHSTEALHAVLKNPPINTKNQNVKDRAEGLVLKVLSAFKSSDIEKAVQSLDKNGVDLLMKYIYKGFERPSENSSAVLLQWHEKALTAGGVGSIVRVLTARKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQP6</accession>
    <name>A0A3B5LQP6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">B box-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQP6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014617.1">
        <property type="protein sequence ID" value="ENSXCOP00000014433.1"/>
        <property type="gene ID" value="ENSXCOG00000010944.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234583"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="15"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="6">
        <location>
            <begin position="69"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="22776" checksum="35F247162B3F2BC7" modified="2018-12-05" version="1">MEADTACQDVDGSCCAVCGETFLAPVVLSCIGCGGSFCNFCLEQFWTQLCVKECPLCFKDSLGLPYKSCKAHGERMLLFCVADLEPVCCVCSRSGHHMNHTVFPIREAFRNHKVGESFRSLKPKHTEAQIKEEFEALHKFLKEQETARLLDLKAEEDQKNLMISEKIEEMSNEVASLSNIIKAAEQEMNSQDIPFLKVKHLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRR2</accession>
    <name>A0A3B5LRR2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 11">Adenylosuccinate synthetase</fullName>
            <shortName evidence="9">AMPSase</shortName>
            <shortName evidence="9">AdSS</shortName>
            <ecNumber evidence="9 11">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Plays an important role in the de novo pathway of purine nucleotide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9 11">
            <text>IMP + L-aspartate + GTP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9 11">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 11">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="9 11"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRR2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014753"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014939.1">
        <property type="protein sequence ID" value="ENSXCOP00000014753.1"/>
        <property type="gene ID" value="ENSXCOG00000011159.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015553"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046040">
        <property type="term" value="P:IMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046716">
        <property type="term" value="P:muscle cell cellular homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048741">
        <property type="term" value="P:skeletal muscle fiber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.170.10:FF:000001">
        <property type="entry name" value="Adenylosuccinate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.300.10:FF:000002">
        <property type="entry name" value="Adenylosuccinate synthetase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="entry name" value="Adenylosuccinate Synthetase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033128">
        <property type="entry name" value="Adenylosuccin_syn_Lys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002223">
        <property type="entry name" value="PRK01117.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00184">
        <property type="entry name" value="purA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846:SF2">
        <property type="entry name" value="ADENYLOSUCCINATE SYNTHETASE ISOZYME 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00513">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 9 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 9 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 9 11" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="43"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="44"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="69"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="71"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="332"/>
            <end position="338"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="364"/>
            <end position="366"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="446"/>
            <end position="448"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="458" mass="50498" checksum="ACB6C87E9CF2A025" modified="2018-12-05" version="1">MSNLSKQKNSLCLNLFIYSTGGQKRVRNDAGNKVTVVLGAQWGDEGKGKVVDLLATEADIICRCQGGNNAGHTVVVEGKEYDFHLLPSGIINTKGISLIGNGVVIHLPGLFEEGDKNEKKGLKGWEKRLIVSDRAHLVFDFHQVVDGLQETERQAQEGKNIGTTKKGIGPAYSSKASRIGLRVCDLLGDFSDFSTRFKNLVHHYQSMYPSLTVDADDQLKKLKDFAERLRPMVRDGVYYMYEALHGPPKKILVEGANAALLDIDFGTYPFVTSSNCTVGGVCTGLGIPPLNIGDVFGVSKAYTTRVGIGAFPTEQLNAVGELLQTRGHEVGVTTGRKRRCGWLDLVILKYAHMINGFTAIALTKLDILDVLDEIKVGVAYKLSGKRIPHFPANMDVLHKVEVEYETFLGWKTDTSAARKWNDLPAKAQNYIRFIENHIGVPIKWVGVGKSRECMIQMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LS16</accession>
    <name>A0A3B5LS16_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LS16"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013595"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013762.1">
        <property type="protein sequence ID" value="ENSXCOP00000013595.1"/>
        <property type="gene ID" value="ENSXCOG00000010289.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154851"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF46">
        <property type="entry name" value="INTERLEUKIN 5 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5027597772" description="Fibronectin type-III domain-containing protein" evidence="9">
        <location>
            <begin position="18"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="10">
        <location>
            <begin position="112"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="327" mass="37209" checksum="65E8B5420D5B1E32" modified="2018-12-05" version="1">MMLWLLFFLLPLGGSSSEEADPGAPGTSATSRPQIYYCRSPNMEDFTCWWLWTSCHWCPDYTSGGLHSCYFDKSHTSIWKYYCMTVTAMTPHRNYTSQQHCLDVAEIVETEAPVNLSSSLRDAGGDEVGHSALLSWTYPAPEDLRYGWITLVYELQYRRIGEDDNWKAKHPLREAHVELLGLRPGDYVVRVRCRSHNSRLWSKWSAALLVSVPNRPPAGKVLVLVLVVGVGAVALLVVAFGVIPQSRRIKDYFLPPIPKPRIMGIDPLLLKKGNFDEINRHFSNFHGYRPPGYSVELWDQVRISEISVENIIHNQSLHENDSSCRKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUJ6</accession>
    <name>A0A3B5LUJ6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 subunit J</fullName>
            <shortName evidence="6">eIF3j</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">eIF-3-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">eIF3 p35</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">EIF3J</name>
        <name evidence="6" type="synonym">EIF3S1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eIF-3 subunit J family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUJ6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014525"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014710.1">
        <property type="protein sequence ID" value="ENSXCOP00000014525.1"/>
        <property type="gene ID" value="ENSXCOG00000011013.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018400"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.60:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.60">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03009">
        <property type="entry name" value="eIF3j"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023194">
        <property type="entry name" value="eIF3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013906">
        <property type="entry name" value="eIF3j"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08597">
        <property type="entry name" value="eIF3_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00065260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="206" mass="23882" checksum="A1024465D7544332" modified="2018-12-05" version="1">EEEEKKVEVKKTETKVSEKKRLTEKIKEKENQLRKKQEEQKKETEENPEEFTQEEQLAEKLRVKKLQEDADLELAKDAFGIQNPLKCVNTVAGIDAMCPSSKEEFTEFEKLLKEKITQFEKSVHYSSFLDSLFRELCISLEVEDLKKISSSLSVLLSEKQKQEKQNKGKKKKKGVVPGGGLKAQMRDDLDYAEFDGGYAQDYEDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUX8</accession>
    <name>A0A3B5LUX8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Translocase of outer mitochondrial membrane 6 homolog (yeast)</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="64" mass="7125" checksum="3085ED644F30F9EB" modified="2018-12-05" version="1">MSGSNVKGSFPSRVSAWISSTYRFATDRNDFRRNLLVNLGLFAAGVWVARNLSDFDLMSPQPVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LW06</accession>
    <name>A0A3B5LW06_9TELE</name>
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        <submittedName>
            <fullName evidence="10">Regulator of G protein signaling 7a</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
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            <location evidence="2">Cell membrane</location>
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        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
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            <topology evidence="1">Peripheral membrane protein</topology>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="6" id="KW-0734">Signal transduction inhibitor</keyword>
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        <location>
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            <end position="86"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="438" mass="51405" checksum="FCB34451B69C7FE5" modified="2018-12-05" version="1">MEELIARMQDEKNGIPIRTVKSFLTKIPSVFSGSDIVQWLMKNLDIEDQVEALHLGTLMAAHGYFFPISDHVLTLKDDGTFYRFQTPYFWPSNCWEPENTDYAVYLCKRTMQNKARLELADYEAESLARLQRAFARKWEFIFMQAEAQAKVDKKRDKIERKILDSQERAFWDVHRPVPGCVNTTEVDIKKSSRMKNPHKTRKSVYGLQNDIRTHSPTHTPTPEAKQPTKEELQEQITFWQLQLDRHRLKMSKVAESLLGYTEQYVEYDPCLTPQDPSNPWMSDDTTLWELEASKEPGQQRVKRWAFGIDEVLKDPVGREQFLKFLESEFSSENLRFWLAVQELKKRPIREVPTRVQEIWQEFLAPGAPSAINVDSKSYDKTTQNVKDPGRYAFEDAQEHIYKLMKSDSYSRFIRSSAYQELLQAKKKVQENASLNFRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWB0</accession>
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        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Xiphophorus couchianus</name>
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        <scope>IDENTIFICATION</scope>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51290">
        <property type="entry name" value="CRIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="5">
        <location>
            <begin position="11"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="CRIC" evidence="7">
        <location>
            <begin position="84"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="203"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="463"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="331"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="387"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="586"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="681"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="758"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="348"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="388"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="407"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="597"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="622"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="643"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="727"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="776"/>
            <end position="788"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="914" mass="103388" checksum="299E52F9E7C19A2E" modified="2018-12-05" version="1">MALVMEPISKWTPKQVLDWMKGLDDCLQQYVKSFEREQIGGEQLLHITHQELEELGVTRIGHQELILEAVDLLCALNYGLETENLRTLSHKLNASAKNLQNFILGRRRGGHYDGRASRRLPNDFLTSVVDLIGAAKNLLAWLDRSPFASVTEYSLLKNNIVQLCLELTTIVQQQQLFFRFSAKLCIVSNVCSFSGLFPLFFQGMYIKSTYDGLHVITGTTENSPADQCKKIHAGDEVIQVNHQTVPIPTSPTSTSPTPVGTLGMSKMDAPSMQDVYILSPVSGLYSTREEMGLMSCDDIGHYSVGSQSGSKGSEAVSYYLDQDSGQYFSLLEGDGPAGTDYDRCRSTGVRRRDKTPTHGKAHRKNSDNSLLRYLSDDKILVIEEEPEGLGRESRRDSTRRSRRKSRNPSSPSFPISPSMLSSTLRLDQPPEPLPVRTKTRKTFQSCSLTSASRRRISCKDLGHGDCEGWLWKKKDAKGYFTQKWRKYWFILKESCLYWYTNQNDEKAEGFISLPEFRIDRAIECRRKFAFKACHPKIKSFFFLLLSFLLVNSHACVPFVFVLTSDYWSESDQDEVDGSMTLKQEGPSSLCDTYHRTPSVSNVNSSSPFPESKHGRHFSSESTHSHSSAEDQRGDGMGMGTGTGSTSTHSSSCRSSHRERRSWQDLIETPLTSAGLHFLQTAPGESEQGMSPERRRQATLPVRRHHAAERDRERDGPFPLERGPYTHNHTHRRSHKQRSQSLPRNRDPILGKFLHSAAHMEERSEDEEAESSAADMLEAEDDGRDPEPLDGLEQLYRALEQANVNALGDPRPCSKQELRRCFTQRVRDPLLNDRLHRVRALRSTLKAKESELEVICALLEDPTLCSQTFREWKQWHSELYAEICQLSPGTNGQDDLSPMGAPLMTHTHSFIETHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWI8</accession>
    <name>A0A3B5LWI8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Group XIIB secretory phospholipase A2-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Group XIII secretory phospholipase A2-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">sPLA2-GXIIB</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Not known; does not seem to have catalytic activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phospholipase A2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWI8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015250"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015441.1">
        <property type="protein sequence ID" value="ENSXCOP00000015250.1"/>
        <property type="gene ID" value="ENSXCOG00000011555.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008798"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070328">
        <property type="term" value="P:triglyceride homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.90.10:FF:000003">
        <property type="entry name" value="Group XIIB secretory phospholipase A2-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010711">
        <property type="entry name" value="PLA2G12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12824">
        <property type="entry name" value="GROUP XII SECRETORY PHOSPHOLIPASE A2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12824:SF2">
        <property type="entry name" value="GROUP XIIB SECRETORY PHOSPHOLIPASE A2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06951">
        <property type="entry name" value="PLA2G12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017345059" description="Group XIIB secretory phospholipase A2-like protein" evidence="13">
        <location>
            <begin position="22"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="22"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="211" mass="22954" checksum="E97E8B9CF297FCC7" modified="2018-12-05" version="1">MTRWALVVPLLVGLLTLRAAGQEAGNPEASPPPPGPPSDGSTQAGDESEDWGLNSLRGGFEAVSGYFDSMLEFMGGKDGVCQYRCRHGKAPLPRPGYNMPEPDGCTSYFFGLPVPDGMDVGIPAMTKCCNQLDTCYDTCGSNKYRCDSKFRWCLHSICSDLKKSLGFVSKVEACETVADTLFNTVWTLGCRPYMNSQRAACYCPGEEKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXD5</accession>
    <name>A0A3B5LXD5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3'-phosphate/5'-hydroxy nucleic acid ligase</fullName>
            <ecNumber evidence="1">6.5.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:68076"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10">Binds 2 manganese ions per subunit.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.8" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXD5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015812"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016007.1">
        <property type="protein sequence ID" value="ENSXCOP00000015812.1"/>
        <property type="gene ID" value="ENSXCOG00000011944.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155911"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072669">
        <property type="term" value="C:tRNA-splicing ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170057">
        <property type="term" value="F:RNA ligase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1860.10">
        <property type="entry name" value="tRNA-splicing ligase RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001233">
        <property type="entry name" value="RtcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036025">
        <property type="entry name" value="RtcB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118:SF1">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01139">
        <property type="entry name" value="RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103365">
        <property type="entry name" value="Hypothetical protein PH1602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="GMP-histidine intermediate" evidence="8">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="77"/>
            <end position="78"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="152"/>
            <end position="155"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="228" mass="25073" checksum="96837C5AEC36BBBA" modified="2018-12-05" version="1">MEKAMKRDKITVNDRQLACARITSQEGQDYLKGMAAAGNYAWVNRSSMTFLTRQAFSKVFGTTPDDLDMHVIYDVSHNIAKVEEHMVDGKQRTLLVHRKGSTRAFPPHHPLIPVDYQLTGQPVLIGGTMGTCSYVLTGTNQGMTETFGTTCHGAGRALSRAKSRRNLDFQDVLDKLADMGIAIRVASPKLVMEEQTVDLNVTDVVNTCHDAGISKKAIKLRPIAVIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZA2</accession>
    <name>A0A3B5LZA2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSXCOT00000016699.1">
        <property type="protein sequence ID" value="ENSXCOP00000016492.1"/>
        <property type="gene ID" value="ENSXCOG00000012449.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158290"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000621">
        <property type="entry name" value="Immunoglobulin superfamily member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000076">
        <property type="entry name" value="Leucine-rich repeat and Ig domain-containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001377">
        <property type="entry name" value="Matrix remodeling associated 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000063">
        <property type="entry name" value="neural cell adhesion molecule L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050958">
        <property type="entry name" value="Cell_Adh-Cytoskel_Orgn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45080">
        <property type="entry name" value="CONTACTIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45080:SF30">
        <property type="entry name" value="HEPARAN SULFATE PROTEOGLYCAN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="246"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="353"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="1244"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="1342"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="1440"/>
            <end position="1532"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="1540"/>
            <end position="1633"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="1641"/>
            <end position="1736"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="1742"/>
            <end position="1832"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="1835"/>
            <end position="1932"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="1938"/>
            <end position="2017"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="2034"/>
            <end position="2120"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="2130"/>
            <end position="2220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="481"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="977"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1033"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1159"/>
            <end position="1181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="488"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="503"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="519"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="978"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1074"/>
            <end position="1091"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="2225" mass="246194" checksum="79B5CE8283D34834" modified="2018-12-05" version="1">SFLYYPALFIYLEKSMNSITEKNINEQTKQGQLCPVCETPALHHGQSVFILHRDAFVCTKPWIKSHLKQKNISLVEGDFIPVYPKDFIAPLGSIQMNLTDQSYQDASLTCTVQTPTAFENLTQTATEDEGRVVTGLTTGITTYLVCNVDHEHVQQLWQILATYSDSPMRLERGVMLARTPEMIYRYSQAKAGEEGITTNIKADIKATPAWLMQGEVSLQLDRTTTTFSTLHIKYQSIVSLHLEAIPTKRGRYSWTMIKRDNQTKTEHIVIAGGVAKLSCHIQGDPKPLMEWILPDGSKVRAPFYSEDRRIVISADGVLTLRGADASDTGLYRCIATNYLDADVLTFRVTVLSPDVEEAGVNGLQLSKSTDDKLTLDCSSTGSPRGSVSWILPDHSVLDKSQANRKVYENGTLLIQGLTARDRGFYRCLVANHLGIDLLVSQVTLSAERFEGMTDLKSEGSGMATDSKMDSSLEDHTVKLKENAFSAPSEKTSQESRTVTSDRSYPRLRLEGRGSPHGRMGQRRRGAAHNRRIWSSRVFAQASRKIDPQKFAKLMEKAHDGSKVKTSTVNESVKNKNPNTVYIAEEETGSGGGYKEEHLIVAPWKVKPTTETMETYKPIKPVYTHIPSQFRITEFYPLERTSVPPTDLNPVTLQLGRRDDTQVTHLSILEQPTAKMETSTGAALALTTDPNVTPVMDDPDPLELVLHTDPKSQMTFTAITTTEREKDIITFHTTQTIKSPQLPAGSTIISKQQIKIIPHKSNRGKGGRRTLHGRRRLIKPNRITDIKAFINRFKQPSVKKEENTTGKTVQLLSAVFCFLGGFLKTGTTNAQPVTPTLSSNLFLSKRERPTSTQKTATSVTNHYIANTPVIHAQSGSEVSSDYITSAEAPEIYSTICPFPTPKEEQQISTTKTNTTKSKVIHGKIPWNRLFESSERVKLLGKLKKPFVQPKATVQTTSVTTLPTDETTTMTIPQGILWSLSPSKSANGKESSLDDDEGLASGDFEFTTLGTEYYPTKTSASDYSRSLDTAQTPTEIQTLPAPPTVDQHSVDNPDRVLTSGSGGSSNQLASNQSFGGRRRQQGRRKKPFGGRRLFKRPKFTKVISKTNVVSTGEVTTKKNANDETTQSVSFYSPAYTISNPHHTTTLMPRTTKQPYVTVQSRVSNSPHRTIEPIENSSNAKGKAEKGLAFDTMTTFTSEEDNNVLSKDKKVTYNSIPGAPNHFMDNEATTARTVPLNPTAKPTRSKPRIVGGNAASFTVLSNSDAFLPCKAVGNPPPVMTWKHFSSFTGNTISISEKTGRFEVLSNGTLTIQNANIKDRGQYLCLAENDLGSDKLLVTLSVVAYPSRILEPKMREIKSQTGDRVEMKCKAEGRPLPMISWILANRTQVRGENTERGRVLVSSEGTLIIDHVSVYDRGYYKCIASNPAGADTATVRLQVVAAPPGIVEQKRQQLKARWRQNLWLPCSSYGTPHPSIHWVLHDGSVVRSHQRMSEKRISVLANGTLFIKDVNAEDDGKYECIATSSTGSERRVVTLSVEKQEHPPEIVETSPNMTELSFGDQLRLDCSATGEPKPKILWRLPSKAVVDQWHRMSSRIQVLVNGTLVVNTVSDKDAGDYLCVAQSPIGDALQFTRVTVSMKPAKIEPKFNRKKQVHYGKDLRVDCKASGAPKPEISWGLPDGTMVNSALQADAGHGGGRARRYTLFENGTLFLNQVSTSEEGDYTCYAENQVGKDEMHVHITVVTAAPRIRPPSQTYARVKPGGNIRFDCETFGEPKPKILWRLSNNDVIAASNERYLIHANGSLGIRDVTLIDAGEYVCMARNPVGENRRIYKLDIDGDPPVINGYQQNRTVMKDVAIKHSRKLLDCKAEGDPTPTVTWIMPDSIFLSAPYFGSRINVHHNGTLEIRNVRPSDSAEFICLARNDGGEAVMVVQLEVTNVLRRPIFKNPFNERIMSRTGKTTILNCSADGQPRPEIIWTLPNGTRITSGSRHSSRLIPGDDGTLVIYSSREEDSGKYRCGARNFVGYIEKLIILNIGQKPYIFTRPKGVIRSISGEPLYLHCLSDGSPRPRIYWTIPGGHTFSQPQILGRYHLLENGTLVVRDTTVHDKGNYICRARNNAGEALITVPVLVIAYPPQITMGSPPRLTAIIGTPLKLNCIATGIPKPEITWELPDHSVLSTAEQGRPMGNELLHPQGTLVIQRPSSSDSGQYKCSAKNYLGTDSKVTFVLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZD6</accession>
    <name>A0A3B5LZD6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZT6</accession>
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            <fullName evidence="13">Metal transporter</fullName>
        </recommendedName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Vertebrata</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        <scope>IDENTIFICATION</scope>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="13">Metal transporter.</text>
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        <subcellularLocation>
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            <topology evidence="1 13">Multi-pass membrane protein</topology>
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    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
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    <keyword evidence="7 12 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017381648" description="Metal transporter" evidence="15">
        <location>
            <begin position="28"/>
            <end position="801"/>
        </location>
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            <end position="205"/>
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        <location>
            <begin position="239"/>
            <end position="260"/>
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            <begin position="266"/>
            <end position="285"/>
        </location>
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            <begin position="297"/>
            <end position="317"/>
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    </feature>
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        <location>
            <begin position="177"/>
            <end position="357"/>
        </location>
    </feature>
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        <location>
            <begin position="444"/>
            <end position="510"/>
        </location>
    </feature>
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        <location>
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            <end position="656"/>
        </location>
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            <begin position="91"/>
            <end position="113"/>
        </location>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50042"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="801" mass="89981" checksum="DBD18B4990C17175" modified="2018-12-05" version="1">MASLSRVRALAMIFLTVTGCCVTPTSGKEGSEETVIIGLRLEDTDDISFMDKGYLRVSERSRVKLRVYGQNINNETWSKIAFTEHERSRAVGGFDSSSGDNQSREDSSGSHPCGIRTSDIIILPNIILSRKTSGVVEIEVKPLRKTERSKAYYLCINTWIYHDGDDTKVIVVEEKKFLLPFWLQVIFISMLLCLSGMFSGLNLGLMALDPMELQIVQNCGTEREKNYAKKIEPVRSQGNYLLCSLLLGNVLVNTTLTILLDDIAGSGLIAVVMSTIGIVIFGEIVPQAICSRHGLAVGANTIFLTKFFMLLTFPASYPVSKLLDYLLGQEIGTVYNREKLLEMLRVTDPYNDLVKEELNIIQGALELRTKTVEDVMTPLRDCFMMPGDTILDFNTMAEIMRSGYTRIPVFEGERSNIVDLLFVKDLAFVDPDDCTPLKTITKFYSHSLHFVFNDTKLDAMLEEFKKGTSHLAIVQRVNNEGEGDPFYEVLGIVTLEDVIEEIIKSEILDETDLYTDNKTKKKITHRDRKQDFSAFKPTDNEMKVKISPQLLLAALRFLATELEPFFPAQMSEKILLRLLKLPNVIQELKYDANNKRATGHYLYHRNKPVDYFILVLQGKVEVEAGKEEMKFEAGPFSFYGMMALTAPPENKSPPRSFGLNHSDSLNRSDRIEAITPTLGNSNNQLNSLLHMYVPDYSVRARSDLRYIKVTRQQYHNAVMASRMDKTPQSTDSELTKIELTLTEFHDGVEIPAIVTTTASTTSTSPAVAVATANETSHLLNQQQNCVGLSRSNHSAHNEGHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZZ5</accession>
    <name>A0A3B5LZZ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">High mobility group protein B2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">High mobility group protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HMGB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZZ5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017428"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017650.1">
        <property type="protein sequence ID" value="ENSXCOP00000017428.1"/>
        <property type="gene ID" value="ENSXCOG00000013144.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153299"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21978">
        <property type="entry name" value="HMG-box_HMGB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21979">
        <property type="entry name" value="HMG-box_HMGB_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000006">
        <property type="entry name" value="High mobility group protein B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000018">
        <property type="entry name" value="High mobility group protein B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF3">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09011">
        <property type="entry name" value="HMG_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00886">
        <property type="entry name" value="HIGHMOBLTY12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="14 22" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0391">Immunity</keyword>
    <keyword evidence="18" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="9" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="19 22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0558">Oxidation</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <keyword evidence="16" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="24">
        <location>
            <begin position="9"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="24">
        <location>
            <begin position="96"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="22">
        <location>
            <begin position="9"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="22">
        <location>
            <begin position="96"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="163"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00040400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00041514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="199" mass="23137" checksum="54940EDC3FF20120" modified="2018-12-05" version="1">MMRKDVNKPKGKTSAYAFFVQTCREEHRKKNPEQSVNFAEFSKKCSERWKALSPGEKKRFEDMAKADKVRYNREMRDYVPPKGFGKRGRKRKDPNAPKRPSAFFVFCSEYRPSVKQQYPGLSIGDCAKKLGEMWSKLGQTEKLPYEEKAQKLREKYDRDMVAYRGGGTFARNPDSSAQGGEEEEDEEGEEDDEDEEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M1S0</accession>
    <name>A0A3B5M1S0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M1S0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017352"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017573.1">
        <property type="protein sequence ID" value="ENSXCOP00000017352.1"/>
        <property type="gene ID" value="ENSXCOG00000013076.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183109"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021684">
        <property type="entry name" value="WBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051994">
        <property type="entry name" value="WW_domain-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16209">
        <property type="entry name" value="VESICULAR, OVEREXPRESSED IN CANCER, PROSURVIVAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16209:SF5">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11669">
        <property type="entry name" value="WBP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="332" mass="36143" checksum="6800F956BE52020A" modified="2018-12-05" version="1">MEYHSGGSLPLLGRPRYCPGANANGGYLCETGHCCGETGCCTYYYELWWFWLLWTVLILFSCCCAYRHRRAKLRVQQQQRQREISLLAYHGANSFPSSMLDLSFLASLKLPSYEEVAAQPSTPPPPYSSVFTTPRYPQPSESGSPTLAPVPVDGSSSCPAITIECETCFMSSPSPLAVLSPSSPRSPLVAPALLLDPLTALHPSDKGGSSSGHASSLSPSPRATQSPPKQTVFSPCVDVFEPGPAEEDEDDDMGADESQYRHRRLTGDSGIEVCRCRVEEEDEDEEEEEEKDGDTDVHDSVDCPARSQLSTGDELTPSRYLDLGEAEEDASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M240</accession>
    <name>A0A3B5M240_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA/RNA-binding protein Alba-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone-like Alba family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M240"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017355"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017576.1">
        <property type="protein sequence ID" value="ENSXCOP00000017355.1"/>
        <property type="gene ID" value="ENSXCOG00000013077.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002564"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="entry name" value="Alba-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051958">
        <property type="entry name" value="Alba-like_NAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13516:SF5">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13516">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="AlbA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding protein Alba-like" evidence="4">
        <location>
            <begin position="53"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="222" mass="24311" checksum="0CD81DF392894D0C" modified="2018-12-05" version="1">KTWVTKHEHHLSLSLNEASPTAVKLGQEGFKKVGRIEEDSPCPFPGLAAGVLEMRVKEGSKIRNLMGFAMARIQGEKTGSAVGGTVTGLRQVVFTGSGRAVTKTITCAEIMKRKVGCLHQMTKLRYKVVKEVWESNQGTASEMTVHRTVPSISILLSKDPLDPQEPGYQPPETLSALWEDRAGTEPSAQTTYKRPLGQLPYSGFPHCKKLCLEEEVSVLPPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3B7</accession>
    <name>A0A3B5M3B7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent RNA helicase SUPV3L1, mitochondrial</fullName>
            <ecNumber evidence="5">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Suppressor of var1 3-like protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
            <location evidence="3">Mitochondrion nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="5"/>
    <dbReference type="RefSeq" id="XP_027871664.1">
        <property type="nucleotide sequence ID" value="XM_028015863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3B7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015926"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016123.1">
        <property type="protein sequence ID" value="ENSXCOP00000015926.1"/>
        <property type="gene ID" value="ENSXCOG00000012026.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114143635"/>
    <dbReference type="KEGG" id="xco:114143635"/>
    <dbReference type="CTD" id="6832"/>
    <dbReference type="GeneTree" id="ENSGT00390000003100"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045025">
        <property type="term" value="C:mitochondrial degradosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000965">
        <property type="term" value="P:mitochondrial RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17913">
        <property type="entry name" value="DEXQc_Suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18805">
        <property type="entry name" value="SF2_C_suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1740.140:FF:000001">
        <property type="entry name" value="ATP-dependent RNA helicase SUPV3L1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1080:FF:000001">
        <property type="entry name" value="ATP-dependent RNA helicase SUPV3L1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000269">
        <property type="entry name" value="ATP-dependent RNA helicase SUPV3L1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000446">
        <property type="entry name" value="ATP-dependent RNA helicase SUPV3L1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1740.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055206">
        <property type="entry name" value="DEXQc_SUV3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022192">
        <property type="entry name" value="SUV3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041082">
        <property type="entry name" value="Suv3_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044774">
        <property type="entry name" value="Suv3_DEXQc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041453">
        <property type="entry name" value="Suv3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE SUPV3L1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22527">
        <property type="entry name" value="DEXQc_Suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12513">
        <property type="entry name" value="SUV3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18147">
        <property type="entry name" value="Suv3_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18114">
        <property type="entry name" value="Suv3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1135">Mitochondrion nucleoid</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="357"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="684"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="746"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="755"/>
            <end position="764"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="773" mass="86432" checksum="3C2D8C8BB39D68E5" modified="2018-12-05" version="1">MSANRTVYFVFSRLQCIFNTRTAHTTAGGCRVISGSRLLLQHSLPGSAACCRNVSSKSVPPKAPDTSLFVPVSLKTDNPADGTVGAELTQPLDKNELLKVLNKFYKRKEMQKLAADHGLDARLFHQAFISFRKYVLEMTALPADLHIILSDICCNAGHIDDIYPYFMRHAKQIFPMLDCMDDLRKISDLRVPANWYPEARAIQRKVIFHSGPTNSGKTYHAIQRYLAAKSGVYCGPLKLLAHEIFEKSNGAGVPCDLVTGEERTFVDPEGRAAGHVACTIEMCSVNTPYEVAVIDEIQMIRDPSRGWAWTRALLGLCAEEIHVCGEAAAVDFIRELMFTTGEEVEVHTYKRLTPFSVLDHAVETLDNLRPGDCIVCFSKNDIYSLNRQIEARGQECAVIYGSLPPGTKLSQAKKFNDPDDPCKILVATDAIGMGLNLSIRRIIFNSLVKPNINEKGEKQMETISTSQALQIAGRAGRFSSVFKEGEVTTMHRDDLPVLKEILSHSVDPIETAGLHPTAEQIEMFAYQLPDATLSNLVDIFVSLSQVDGLYFVCNIDDFKFLADMIQHIPLNLRSRYVFCTAPINKKQPFVCTSFLKFARQFSRDEPLTFDWVRRHIGWPLAAPRNIKDLVHLEAVHDVLDLYLWLSYRFMDMFPDTAAIRGIQQELDNIIQQGVRNITRLIRASDPNLSDPPEQNAAAAAGSRSRSRGQRSSTGTMEKSLASRLVKDGLLTPDLLKQLQREWSKSLRQEGGGQSLLGEELNNKSIKGKRKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5N8</accession>
    <name>A0A3B5M5N8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M5N8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018757"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018993.1">
        <property type="protein sequence ID" value="ENSXCOP00000018757.1"/>
        <property type="gene ID" value="ENSXCOG00000014118.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155489"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF11">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="5">
        <location>
            <begin position="53"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="207" mass="23813" checksum="6C2E4221BEE4A603" modified="2018-12-05" version="1">ISWRSIHRPPVLSEEMEKECQISILDLIYIQTFVSEALAQRDPNFVLSSAFKLRITQRDLSTLLEGGWLNDEVMNFYLSLVMERRGESGRLKVYSFSTFFYPKLRGVVGGEQGGGHVAVKRWTKAVDLFMFDFILVPLHLGVHWALAVMDLKSKTDIPQQTNGSDCGVFVCKYADYIAKGKPLTFKQCHMPLFRKLMMWEILNQKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M668</accession>
    <name>A0A3B5M668_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sodium/calcium exchanger membrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Ca(2+)(out) + K(+)(out) + 4 Na(+)(in) = Ca(2+)(in) + K(+)(in) + 4 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:69967"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M668"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019703"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019948.1">
        <property type="protein sequence ID" value="ENSXCOP00000019703.1"/>
        <property type="gene ID" value="ENSXCOG00000014799.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234532"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060292">
        <property type="term" value="P:long-term synaptic depression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060291">
        <property type="term" value="P:long-term synaptic potentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000002">
        <property type="entry name" value="Sodium/potassium/calcium exchanger 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000004">
        <property type="entry name" value="Sodium/potassium/calcium exchanger 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00367">
        <property type="entry name" value="calcium/sodium antiporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF41">
        <property type="entry name" value="SODIUM_POTASSIUM_CALCIUM EXCHANGER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0769">Symport</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="468"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="503"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="537"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="570"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="600"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="17">
        <location>
            <begin position="137"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="17">
        <location>
            <begin position="469"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="405"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="416"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="630" mass="70005" checksum="DF9932D29FCB73F0" modified="2018-12-05" version="1">MLPIRSQEMIAFSCSDPFLDPHSVNTRFPHQAVKRKLRPGRILGFVISLVAVCTVCSLSAFSLATAVATEQRESVAESPADAAVPQRTLLQRQNVSGAPEDTPVAMKSSHLDMNQGDYPTDYFSVEDRRQGYVVLHMFGMLYMFIALAIVCDEFFVPALTVITEKLEISDDVAGATFMAAGGSAPELFTSIIGVFVSHSNVGIGTIVGSAVFNILFVIGMCALFSKEVLNLTWWPLFRDVSFYIIGLLLLIYFFLDNQITVSESISLLMCYTCYVTFMKFNSQIENLIKTRLGSNQVVELEAAPKVSLAKPRLQREGSCASLHNSLMRNSIFQLMIHTLDPLSNEGSTRGEDTFTDLWFLAGRFKEKASILHKMAKHKCKEEAATANGVVDKKLTNSSKVAVEVTPPMNGVAGGDQEGEEEEDEDQPLSLAWPDTPRKRITYLLILPIVFPLWLTLPDVRRETSERFFPVTFIGSICWIAFFSYLMVWWAHQVGETFWITEEIMGLTILAAGTSIPDLITSVIVARKGLGDMAVSSSVGSNIFDITVGLPFPWLLYNIINDFKPVEVSSNGLFCAIVLLFLMLLFVIISIAACKWKMSKFLGLLMFLLYFVFLVVSVMLEDKVIMCPVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7C2</accession>
    <name>A0A3B5M7C2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">KH RNA binding domain containing, signal transduction associated 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">KHDRBS2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7C2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015694"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015888.1">
        <property type="protein sequence ID" value="ENSXCOP00000015694.1"/>
        <property type="gene ID" value="ENSXCOG00000011857.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157134"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032571">
        <property type="entry name" value="Qua1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032335">
        <property type="entry name" value="Sam68-YY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF34">
        <property type="entry name" value="KH DOMAIN-CONTAINING, RNA-BINDING, SIGNAL TRANSDUCTION-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16274">
        <property type="entry name" value="Qua1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16568">
        <property type="entry name" value="Sam68-YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KHDRBS Qua1" evidence="5">
        <location>
            <begin position="27"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Sam68 tyrosine-rich" evidence="6">
        <location>
            <begin position="223"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="138"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="139"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="171"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="279" mass="32069" checksum="7FA106A9A29FC445" modified="2018-12-05" version="1">MLFWIYPWTFIFDFSLFLNKLFEFHQLHPSFLSIGILVSRIGKYEGDELKKDADTKKYLDIISNKNIKLSERVLIPIQQYPKVRNYSFKLLDYFSCNANTLCLFVLLSLSMPPGQIHRKANKDYNDEIRQEQLRELSLLNGSDESSRGRSIQGRSLRTTTTASTMGHGGASRRAATPRGTAAATHSKLPKTALTRDLQAPRIRGAAANAGYRPPLLAVTHESYDDYGYEDGYDEEYEDETYEEYDENYRTQTKSPRPILKAPALRLTRGGYRKHPYGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M899</accession>
    <name>A0A3B5M899_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M899"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017526"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017748.1">
        <property type="protein sequence ID" value="ENSXCOP00000017526.1"/>
        <property type="gene ID" value="ENSXCOG00000013213.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156166"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="zinc finger protein Gfi-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000208">
        <property type="entry name" value="zinc finger protein Gfi-1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000432">
        <property type="entry name" value="zinc finger protein Gfi-1b isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="128"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="156"/>
            <end position="183"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="184"/>
            <end position="211"/>
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    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
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            <end position="238"/>
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    </feature>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="238" mass="27172" checksum="AFC2C5CC79A48429" modified="2018-12-05" version="1">MPRSFLVKRGGLHHLRVAVRSPSPGSTPVVFGKLEKNVESWDASLGYSAAKEPDEKQASILTSQADIYVSSCTDANMQLQQFSPKSSEVFTQGQGQSHRCFTDDLYSESSTECCILTTLTFTFQEKTFGCEVCGKVFKRSSTLSTHLLIHSDTRPYPCQYCGKRFHQKSDMKKHTFIHTGEKPHVCQICGKAFSQSSNLITHSRKHRDDRPYRCTLCHYSFQHKVDLRQHQEHHCTYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8A8</accession>
    <name>A0A3B5M8A8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Platelet-derived growth factor C</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PDGF/VEGF growth factor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M8A8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019747"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019993.1">
        <property type="protein sequence ID" value="ENSXCOP00000019747.1"/>
        <property type="gene ID" value="ENSXCOG00000014830.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158645"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005161">
        <property type="term" value="F:platelet-derived growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048008">
        <property type="term" value="P:platelet-derived growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000010">
        <property type="entry name" value="Platelet derived growth factor C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000072">
        <property type="entry name" value="PDGF/VEGF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11633">
        <property type="entry name" value="PLATELET-DERIVED GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11633:SF5">
        <property type="entry name" value="PLATELET-DERIVED GROWTH FACTOR C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00341">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50278">
        <property type="entry name" value="PDGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0339">Growth factor</keyword>
    <keyword evidence="11" id="KW-0497">Mitogen</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017282574" description="Platelet-derived growth factor C" evidence="13">
        <location>
            <begin position="21"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="14">
        <location>
            <begin position="42"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Platelet-derived growth factor (PDGF) family profile" evidence="15">
        <location>
            <begin position="242"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="337" mass="38299" checksum="93E9B7309B488847" modified="2018-12-05" version="1">MILLLIFLLASLLQQYGTEAESSDVSKLQLSNIKEPNQESVQEKVLTVSGEGMIHSPDFPHTYPRDTMLVWRLVAADNMRIQLTFDENFGLEDPEDGICYDFVEIEDPTEKTILGRWCGSQSAPTSQKSKGSQVIVRFISDEYFPSEPGFCLHYSLLTASEPEATTQPPPSLQGVEELLEAVAVLSTVEDVMKYLEPERWQLDMEELYKPTWHVLGKSFILNRKARGADLNLLREEVRLYSCTPRNYSVSLREELKRTDVIFWPSCLLVKRCGGNCACCSHRCIDCQCVPARVTKKYHEQVLLLRHRGGGRSLQKSMTDVALEHHEECSCVCKDDQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9P3</accession>
    <name>A0A3B5M9P3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DnaJ heat shock protein family (Hsp40) member C2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DNAJC2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9P3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020245"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020493.1">
        <property type="protein sequence ID" value="ENSXCOP00000020245.1"/>
        <property type="gene ID" value="ENSXCOG00000015178.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155441"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000180">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily C, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044634">
        <property type="entry name" value="Zuotin/DnaJC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43999">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43999:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23082">
        <property type="entry name" value="Myb_DNA-binding_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="167"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="167"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="6">
        <location>
            <begin position="175"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="240" mass="26825" checksum="B4D3B70D4C251A80" modified="2018-12-05" version="1">MMEEVEKLCDRLELTSLQSLNEILASASKEDSKAAVEKQVEEVNAQLQKEKDAEVQARQAARSAEQASGGAGGGKGWNEDDLQLLIKAVNLFPAGTNARWEVIANYMNLHSTSGMKRTAKDVINKAKNLQKLDPVQKDEINRKAYEKFKKEHTSVPPSIDNALPSERFDGNTAPWTTEEQKLLEQALKTYPVSTPERWEKIAVAVPGRSKKDCMKRYKELVEMVKAKKAAQEQVAAKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAM4</accession>
    <name>A0A3B5MAM4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the paired homeobox family. Unc-4 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027872132.1">
        <property type="nucleotide sequence ID" value="XM_028016331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAM4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021298"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021558.1">
        <property type="protein sequence ID" value="ENSXCOP00000021298.1"/>
        <property type="gene ID" value="ENSXCOG00000015942.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114143924"/>
    <dbReference type="KEGG" id="xco:114143924"/>
    <dbReference type="GeneTree" id="ENSGT00940000161420"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000057">
        <property type="entry name" value="Short stature homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46799">
        <property type="entry name" value="HOMEOBOX PROTEIN UNC-4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46799:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN UNC-4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="99"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="101"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="157"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="367"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="434"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="225"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="461"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="502" mass="54732" checksum="98BDA98B61A3CA09" modified="2018-12-05" version="1">MDSRVLEHPHAQFGGSLGGLVGFPYSLGSHHHHHHHQHVYELASGHQLQSAAAVPFSIDGLLNGSCSASAVSSSPLLSSDSQQFKLSDSGDPDKDSPGCKRRRTRTNFTGWQLEELEKAFNESHYPDVFMREALALRLDLVESRVQVWFQNRRAKWRKKENTKKGPGRPAHNSHPTTCSGEPMDPEEIARRERERAEKKKRKHERKLLKSQNKLLSGDSFHTPGGSESDSGVSQFTDSEQQPPLPSIHVELPASRGESSCDQTRHDFGPPQNQPNQRTMGEPEQVSPGSTHSPGPGGQRSPGMQKRNPFSVESLLSDTMPRRKSQLDFSPLPNQRTLVGKGHFLLYPITHQPLGFLVPQAALKAAPCQENSDRLAQGQRRSPSEGSPAPSDLSAFGSTTDRLNSARMEHTLHNHLGVTAEAGDARVRVGSCKNAAFPVPPPSDGTDQTVLVGEDGGPTSRTEVEQSSKERSAREEPGSPGLAEANTDCGETPGTDGEDVDMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MB26</accession>
    <name>A0A3B5MB26_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Nipsnap homolog 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Protein involved in mitophagy. Accumulates on the mitochondria surface in response to mitochondrial depolarization and acts as a 'eat me' signal by recruiting proteins involved in selective autophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NipSnap family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MB26"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016909"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017126.1">
        <property type="protein sequence ID" value="ENSXCOP00000016909.1"/>
        <property type="gene ID" value="ENSXCOG00000012750.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183018"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000003">
        <property type="entry name" value="Protein NipSnap homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000007">
        <property type="entry name" value="protein NipSnap homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012577">
        <property type="entry name" value="NIPSNAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051557">
        <property type="entry name" value="NipSnap_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21017">
        <property type="entry name" value="NIPSNAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21017:SF14">
        <property type="entry name" value="PROTEIN NIPSNAP HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07978">
        <property type="entry name" value="NIPSNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017288889" evidence="7">
        <location>
            <begin position="32"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="NIPSNAP" evidence="8">
        <location>
            <begin position="174"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="273" mass="32651" checksum="E978CB3280127962" modified="2018-12-05" version="1">MHCIILSCIVAVRDVALRLFFLCSWFRGFSASNSRNREDSWFRSLFVRKVDPRKDAHSTLLTKNEESNLYKIQFHNVKPDEDVLPSIHADKYYPCELVGTWNTWYGEQDQAVHLWRYRGGYPALTEVMNKLRQNKEFTAYRKERGKMLLSRRNQLLLEFSFWNEPMPREGPNIYELRSYQLRPGTMIEWGNYWARAIRYRQRNREAVGGFFSQIGDLYMVHHLWAYKDLLSREATRNAVWQQEGWHEVVYYTVPLIQHMDSRIMIPTKASPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBR8</accession>
    <name>A0A3B5MBR8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBR8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021042"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021300.1">
        <property type="protein sequence ID" value="ENSXCOP00000021042.1"/>
        <property type="gene ID" value="ENSXCOG00000015746.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006548"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_eS17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="76" mass="8639" checksum="FB3BECD5C82D3307" modified="2018-12-05" version="1">MFFCTLKEFLINLNSKLLEIDKENRTVVVVSALDQELIEVDPDTKEMLKLLDFGSLSNLQVTQPPVGMNFKQPRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MD33</accession>
    <name>A0A3B5MD33_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PCI domain-containing protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">CSN12-like protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN12 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MD33"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022173"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022442.1">
        <property type="protein sequence ID" value="ENSXCOP00000022173.1"/>
        <property type="gene ID" value="ENSXCOG00000016565.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001101"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000146">
        <property type="entry name" value="PCI domain-containing protein 2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045114">
        <property type="entry name" value="Csn12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732:SF0">
        <property type="entry name" value="PCI DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732">
        <property type="entry name" value="UNCHARACTERIZED PROTEASOME COMPONENT REGION PCI-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="207"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00026186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00033214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="395" mass="45623" checksum="67ADE78AFB66BAD8" modified="2018-12-05" version="1">MAHITINQYLQQINQAIENHEGSFCAELLSFKHPHVANPRLQLASPEEKCQQILEPPYDEMVAAHLCTYAVANHDFVEAYKFQTLVVSFLRAFQSHKEENALPVMSAVTLDLRIFANNAEQQLQKKGKGQPGEMLEKAAEQLMSCFRVCASDRAGIDDSKKWGMMFLSNQLFKIYFKQINKLHLCKPLIRAIDSSNLKNEYSPAQKVTYKYYVGRKAMFDSDFKTEELLSFAFDRCHRSSQKNKRMILIYLLPVKMLLFQGHMPTHQLLRKYDLIQFADVTKAVEGNLLLLNEALSKHETFFIRCGIFLILEKLKIITYRNLFKKYLLLKTHQLPLDAFLVALRMMQVEDVDIDEVQCLLANLIYMGHIKGYISHQHQKLVVSKQNPFPPLSSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDE7</accession>
    <name>A0A3B5MDE7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ARB2 cotranscriptional regulator A</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDE7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022288"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022561.1">
        <property type="protein sequence ID" value="ENSXCOP00000022288.1"/>
        <property type="gene ID" value="ENSXCOG00000016656.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063907"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035197">
        <property type="term" value="F:siRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048263">
        <property type="entry name" value="Arb2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053858">
        <property type="entry name" value="Arb2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21357:SF6">
        <property type="entry name" value="COTRANSCRIPTIONAL REGULATOR FAM172A HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21357">
        <property type="entry name" value="FAM172 FAMILY PROTEIN HOMOLOG CG10038"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22749">
        <property type="entry name" value="Arb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017418026" evidence="2">
        <location>
            <begin position="25"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Arb2" evidence="3">
        <location>
            <begin position="56"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="349" mass="40149" checksum="906AC2151A62B6AC" modified="2018-12-05" version="1">MSSHLISATSALYILWITMAQVAGQEDREKTTALKDLLSRIDLDELMKKDEPPFTFPKTLEEFEYAFNENGQLRHIQTGEPFVFNAREDLHRWNQKRYEALGEIITKYVYELLEKKCNLTKTFLPVDATDDEPRSFIYLSTDALTNPNKLLVLIQGSGVVRAGQWARRLIINQDLDSGTQIPFIMKAMEENTSSEEHVLYVWDHFVSKAAAKNVFIIAHSYGGLSFVELMNQREMQVKNRVCAVALTDSAHNIWHQETSKGTQDWMQQCCCNWVSSPEPLDTQLEPMLPDCPRVSAGTERHELTSWMSFESIFRFFNEILKTKEEEEAEENANVVTTRSGSLKNKHQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDN0</accession>
    <name>A0A3B5MDN0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">cis-Golgi network membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDN0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019366"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019604.1">
        <property type="protein sequence ID" value="ENSXCOP00000019366.1"/>
        <property type="gene ID" value="ENSXCOG00000014553.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158445"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GOLD" evidence="11">
        <location>
            <begin position="44"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="249" mass="28170" checksum="DE9E05560240B8C9" modified="2018-12-05" version="1">CCLSFTLSVAVRLKQSHPLFDRYTSLLPQSQDSEFTFLLPAGRSECFYQTAIKNGTMEVEYQVIAGAGMDVDFTIISPQGARLIIENRRSDGVHVVEPMEEGDYEICFDNGFSRFSEKMVFFEIIIEGQGGDVGGDDEFPGVEEPDGSLLEYKLDDIRETMDSLHKRLERSRQMQTVLRAFEARDRNLLEDNLWRVSFWSCASVLVMLCVAVTQVTATRLVHSLHFNQASQSMTHVHPPSIFLSISLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEM7</accession>
    <name>A0A3B5MEM7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEM7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019681"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019926.1">
        <property type="protein sequence ID" value="ENSXCOP00000019681.1"/>
        <property type="gene ID" value="ENSXCOG00000014783.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="75" mass="7280" checksum="57A64C47E96B4FCC" modified="2018-12-05" version="1">MEICPIIPAKTRICSFSCCGPVGEAGPQGAPGKEGPPGLRGDHGSPGRQGERGPAGPSGSPGDKGDPGEDGPTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEU0</accession>
    <name>A0A3B5MEU0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Receptor ligand binding region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEU0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022798"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023074.1">
        <property type="protein sequence ID" value="ENSXCOP00000022798.1"/>
        <property type="gene ID" value="ENSXCOG00000017036.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234648"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000196">
        <property type="entry name" value="Glutamate metabotropic receptor 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050726">
        <property type="entry name" value="mGluR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24060">
        <property type="entry name" value="METABOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017406784" description="Receptor ligand binding region domain-containing protein" evidence="7">
        <location>
            <begin position="36"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Receptor ligand binding region" evidence="8">
        <location>
            <begin position="79"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="205" mass="22242" checksum="658D083029654966" modified="2018-12-05" version="1">MPRSGSRAMGRQASRPPRLLRMLWLLPALLDAAGSQEIYAPHSIRVEGDVTLGGLFPVHGRGAPGAPCGDIKKENGIHRLEAMMYALDQINGDEELLPNVTLGARVLDTCSRDTYALEQSLTFVQALIQKDTSDVRCTNGEPPVFVKPEKVVGVIGASASSVSIMVANILRLFQVRLRPGSSGEPRCVSDAVWVRTRPLLFILTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFG0</accession>
    <name>A0A3B5MFG0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Hypocretin neuropeptide precursor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Hypocretin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Orexin precursor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Prepro-orexin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Preprohypocretin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Binds to orexin receptor HCRTR2/OX2R only. Stimulates food intake. Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Binds to orexin receptors HCRTR1/OX1R and HCRTR2/OX2R with a high affinity. Stimulates food intake. Modulates pituitary luteinizing hormone secretion in an ovarian steroid-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Rough endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the orexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MFG0"/>
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    </dbReference>
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        <property type="term" value="P:positive regulation of transmission of nerve impulse"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HYPOCRETIN NEUROPEPTIDE PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0027">Amidation</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0527">Neuropeptide</keyword>
    <keyword evidence="8" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017284980" description="Hypocretin neuropeptide precursor" evidence="19">
        <location>
            <begin position="47"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034336"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034351"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034354"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034367"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00046224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="128" mass="14110" checksum="428DE71318723029" modified="2018-12-05" version="1">IITHHTRVKMTWYTTNFHNTAGMDTPNRKTLAFMLILLLAQLGCSTQSVSECCRQPARSCRLQVLLCRSGNKSIGGTIAGDAAAGILTLGKRNEDELHLQSRLHQLLHGRKNQAAGILTMGKRTAQRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFW1</accession>
    <name>A0A3B5MFW1_9TELE</name>
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        <submittedName>
            <fullName evidence="8">Microtubule actin crosslinking factor 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
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            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
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    </dbReference>
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        <property type="entry name" value="MICROTUBULE-ACTIN CROSS-LINKING FACTOR 1, ISOFORMS 1_2_3_5"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00243">
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    </dbReference>
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    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="24"/>
            <end position="59"/>
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    <feature type="domain" description="EF-hand" evidence="6">
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            <end position="94"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="217" mass="25094" checksum="AC3C9500AE22ED50" modified="2025-10-08" version="2">MQMEEFANFDFNIWRKRYIQWISHLKSRILDVFRSIDRDQDGRISRKEFTDYVLASFPTNSLEMNAVANIFDLNNDGFIDYYEFVSALHPSRDPYRKTLDADQINEEVSRQVAQCNCPKRFQVEQISANRYRFGDSQQLRMVRILRSTLMVRVGGGWTALDEFLVKNDPCRKGRTNLKIKEKYLSPTGSTTKGLTVSRSNSSLSLYSSASAPTSPMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGA6</accession>
    <name>A0A3B5MGA6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TRAF-type zinc finger domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Negative feedback regulator that controls excessive innate immune responses. Regulates both Toll-like receptor 4 (TLR4) and DDX58/RIG1-like helicases (RLH) pathways. May inhibit the LTR pathway by direct interaction with TRAF6 and attenuation of NF-kappa-B activation. May negatively regulate the RLH pathway downstream from MAVS and upstream of NF-kappa-B and IRF3.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGA6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023353"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023633.1">
        <property type="protein sequence ID" value="ENSXCOP00000023353.1"/>
        <property type="gene ID" value="ENSXCOG00000017437.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063869"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045824">
        <property type="term" value="P:negative regulation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051986">
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    </dbReference>
    <dbReference type="InterPro" id="IPR049439">
        <property type="entry name" value="TRAFD1-XIAF1_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16295:SF19">
        <property type="entry name" value="TRAF-TYPE ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16295">
        <property type="entry name" value="TRAF-TYPE ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21366">
        <property type="entry name" value="TRAFD1-XIAF1_ZnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRAFD1/XAF1 zinc finger" evidence="9">
        <location>
            <begin position="91"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="567" mass="63029" checksum="0E1A59F349C7F7C9" modified="2018-12-05" version="1">MADENTQFCGNCKHDIPEANFTTHEIHCRRNIGLCDVCQEPVPHSELQEHKIQEHTQITCKCGLKIEKNQSEVHQSSECSQRLVPCPYCELELVFSQSKEHEDYCGARTEPCPNCKCNVMLREKAVHPVLCGSLTPPQERNNNRMGCGQTENRSSDDWFEAHSIRNPLRAQEGAPKNNNISAAERPHFLLPFEPSVYSGSSGDREAGSWKSSTSHSTTSNLCELILNYLIDTEPQESSSVRPPDEESSGLDYDYMLALSLQSDGEAAGGGAEDSVWRNIWDHEDKKTNSSVNSFPLAHNKNTNSPEGTSAVQGSGSADTMLPCEFCEELFPEEDLILHETGCSPASAFASFSKKPSSPPKDDRMGRKASSGLMQSFPNTLASNIPTFPRSVSPASYSPPASPLEGDVVIPCEFCGVALEEAVLFHHQDKCDMRTEAGHQLNNTAIQKPLGPAGDAFGQTSADFQRIKHQGKVGYFFRLLIKIKTIIYLKKMLVLSQILQCYIYILLFHSFKHTSTNCENRCGWSCRCISGCCLLFLHTHDLCSCIHLTFRMKTFGTQNLDKRLEDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MH49</accession>
    <name>A0A3B5MH49_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pre-mRNA-splicing factor ISY1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">Identified in the spliceosome C complex. Component of the XAB2 complex, a multimeric protein complex composed of XAB2, PRPF19, AQR, ZNF830, ISY1, and PPIE. Identified in a pentameric intron-binding (IB) complex composed of AQR, XAB2, ISY1, ZNF830 and PPIE that is incorporated into the spliceosome as a preassembled complex. The IB complex does not contain PRPF19. Interacts with CPSF3; this interaction is in an RNA independent manner. Interacts with the microprocessor complex subunits DGCR8 and DROSHA; this interaction is in an RNA dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ISY1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MH49"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023082"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023359.1">
        <property type="protein sequence ID" value="ENSXCOP00000023082.1"/>
        <property type="gene ID" value="ENSXCOG00000017248.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014109"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.660:FF:000001">
        <property type="entry name" value="pre-mRNA-splicing factor ISY1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009360">
        <property type="entry name" value="Isy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037200">
        <property type="entry name" value="Isy1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13021">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ISY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06246">
        <property type="entry name" value="Isy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140102">
        <property type="entry name" value="ISY1 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00065310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00070851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="115" mass="13310" checksum="6795E4AD35BB86BA" modified="2018-12-05" version="1">QARNAEKAMTALARFRQAQLEEGKVKERRPFLASECSELPKAEKWRRQIISEISKKVAQIQNAGLGEFRIRDLNDEINKLLREKGHWEVRIKELGGPDYRVTPEPSEPEPELMVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHP8</accession>
    <name>A0A3B5MHP8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHP8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023277"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023555.1">
        <property type="protein sequence ID" value="ENSXCOP00000023277.1"/>
        <property type="gene ID" value="ENSXCOG00000017384.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048121"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030681">
        <property type="term" value="C:multimeric ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3250">
        <property type="entry name" value="Ribonuclease P, Pop5 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002759">
        <property type="entry name" value="Pop5/Rpp14/Rnp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
        <property type="entry name" value="Rnp2-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441:SF1">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01900">
        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="Rnp2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="144" mass="16176" checksum="E60315EDC7B8B3CD" modified="2018-12-05" version="1">MFCDLFLYRVSSLQKMRAGTEEAESSGYQRVVLKNASPHHYLKVRLVLEDESARVSAVNLKTFITSCLRSLFGEVGAALNVDVLKYDEETLTALLRVPSRGLVKLWSSLTLQGFYQNRACAFRVLQVSPFLLALTGNSRDLQLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJL4</accession>
    <name>A0A3B5MJL4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Solute carrier family 7 member 10a</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJL4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023962"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024247.1">
        <property type="protein sequence ID" value="ENSXCOP00000023962.1"/>
        <property type="gene ID" value="ENSXCOG00000017892.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156469"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015175">
        <property type="term" value="F:neutral L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042941">
        <property type="term" value="P:D-alanine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042942">
        <property type="term" value="P:D-serine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000008">
        <property type="entry name" value="large neutral amino acids transporter small subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF518">
        <property type="entry name" value="ASC-TYPE AMINO ACID TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="194"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="274"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="476" mass="52221" checksum="2D0740394961D192" modified="2018-12-05" version="1">APPPSTNNVNSVHEGNIIGSGIFISPKGVLEHAGSVGLSLIVWVCGGGICALGSLCYAELGVTIPKSGGDYSYVTEIFGGLVGFLLLWSAVLIMYPTTLAVIALTFSNYVLQPAFQNCLPPFIATRLLATICVLFLTWVNCSSVRWATRIQDIFTVGKLLALVLIIVVGLVQIFKGHYDALRPSVAFEFSQDPSFGQIALAFLQASFAYSGWNFLNYVTEEVVEPRRNLPLAIYISIPLVTLVYTMTNIAYFTSMTPEELLASNAVAVTFGEKLLGMFSWVMPISVALSTFGGINGYLFTSSRLCFSGAREGHLPYLLAMIHLKNCTPIPALLVCCIATVLIMCIGETHNLINYVSFINYLSYGVTIAGLLYLRKKRPNLARPIKVNLLVPITYLIFWALLLFCSLYSEPVVCGLGMVIMLTGVPVYFVGVHWKEKPKWIYILLERVTYMGQKLCYVVFPQADSSETEPLTAKAID</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKY6</accession>
    <name>A0A3B5MKY6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sorting nexin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKY6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025049"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025354.1">
        <property type="protein sequence ID" value="ENSXCOP00000025049.1"/>
        <property type="gene ID" value="ENSXCOG00000018701.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000271729"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="74" mass="8193" checksum="76FF0CC30E4BB92A" modified="2018-12-05" version="1">MADTARDPPPDRTDFHEVEDGEDLFPEPSSQPASLSAVDISTNANGPKQDSFFDDDNEDLFAGMNTHRIIITNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MMJ6</accession>
    <name>A0A3B5MMJ6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein disulfide-isomerase A5</fullName>
            <ecNumber evidence="3">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MMJ6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024781"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025082.1">
        <property type="protein sequence ID" value="ENSXCOP00000024781.1"/>
        <property type="gene ID" value="ENSXCOG00000018507.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156797"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02997">
        <property type="entry name" value="PDI_a_PDIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd03067">
        <property type="entry name" value="PDI_b_PDIR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000029">
        <property type="entry name" value="protein disulfide-isomerase A5 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000105">
        <property type="entry name" value="protein disulfide-isomerase A5 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051063">
        <property type="entry name" value="PDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046374">
        <property type="entry name" value="PDI_a_PDIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041865">
        <property type="entry name" value="PDI_b_PDIR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672:SF2">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="133"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="11">
        <location>
            <begin position="269"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="508" mass="57757" checksum="2AC8E90CC573D0F0" modified="2018-12-05" version="1">CSHSSIKHRHSSMLVLSSSLAAVKVSPLIEKVSDHKDFKKLLRTRTNVLVLYTKTATSGDSYLKLLSDVAQTVKGQGTIAWVNCGDSEGRKLCKKVKVDPSSRREGAELLHYKDGTFHTQYNRPATFKSMVAFLKDPSGPPLWEENPEAKDVVHVETEKDFRKLLKKEERPVLIMFYAPWCGVCKRMQPVFQQAATETKGKYVLAGMNVHPAEFDALKQEYNVKGYPTFCYFEKGKFMHHYENYGATPKDIADWLKNPQPPQPKTPEVPWSETDSPVFHLTDESFDSFLEEHPAALVMFYAPWCGHCKKMKPEYDEAAEVLNKSPGVLAAVDATVHKAAGDRFKISGFPTVKYFEKGEEKYTLPHLRSKDKIIEFMHNPQAPPPPEQSWEDRPSSVSHLGSEDFREALKKKKHALVMFYAPWCPHCKNAVPHFTTAAELFKEDRKVNCLGLFPNLPNSVTHQLSLPHTHTHTHAEETKGTCLAILFATIYHLKLRGMDEKKESNVFWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNY1</accession>
    <name>A0A3B5MNY1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Membrane-associated guanylate kinase inverted 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000021189"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021449.1">
        <property type="protein sequence ID" value="ENSXCOP00000021189.1"/>
        <property type="gene ID" value="ENSXCOG00000015851.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156496"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06730">
        <property type="entry name" value="PDZ0_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06731">
        <property type="entry name" value="PDZ1_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06732">
        <property type="entry name" value="PDZ2_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06733">
        <property type="entry name" value="PDZ3_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06734">
        <property type="entry name" value="PDZ4_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06735">
        <property type="entry name" value="PDZ5_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000005">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000006">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000012">
        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000001">
        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000131">
        <property type="entry name" value="membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316">
        <property type="entry name" value="MEMBRANE ASSOCIATED GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316:SF65">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE, WW AND PDZ DOMAIN-CONTAINING PROTEIN 3 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0796">Tight junction</keyword>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="65"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="15">
        <location>
            <begin position="111"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="284"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="14">
        <location>
            <begin position="330"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="412"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="514"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="687"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="808"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="16">
        <location>
            <begin position="952"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="217"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1075"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1148"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1268"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1076"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1189"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1215"/>
            <end position="1225"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1233"/>
            <end position="1243"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1244"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1268"/>
            <end position="1294"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1297"/>
            <end position="1310"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00016171"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00033438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
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        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1336" mass="149060" checksum="6A7DA15880251AB9" modified="2018-12-05" version="1">MSRTLKKKKHWSTKVVECAVSWGSLGDFGSVVEILGGAELGQFPYIGQMKLDVLVCHVGKLPYYGDVLLEVNGTPVSGLTNRDTLAVIRHFREPIRLKTVKPGKVLNTDLRHYLSLQFQKGSLDHKLQQIIRDNLYLRTIPCTTRLPREGEVPGVDYNFISVGDFRILEESGLLLESGTYDGNYYGTPKPPAEPNLVQPDLVDQVLFDEDFSGEVPRKRTTSVSKMDRKDSAVPEEEDDDERPPLVNGLPEHKDWRKTVPSYNQSSSTMDFRTWSSLPRDDSLEPLPYNWEMAYTETGMVYFIDHNTKTTTWLDPRLAKKAKPPEKCEDDELPYGWEEIDDPQYGTYYVDHINQRTQFENPVLEAKKKLSKDTASIQQAAAASSQGQICSFCKGSMLGFTADPSQLKGEMYHTALKKSSQGFGFTIIGGDRTDEFLQVKNVLSDGPAALDNKMASGDVIVEINGTCVLGKTHPEVVQMYQSIPINQYVDMVLCRGYPLPPDQPLHGMLQPELVSVPLVKGPGGFGFAIADCPLGQKVKMILDAQWCRGLQKGDVIKEINRQNVQSLSHAQVVDILKDFPVGSEVSVLVLRGGQTSPVKSLKPVSKNALFPLSYPLFPHGDISFNLISKHFVLHTETLDTIADATPQALPYHSNTSTLRSSDSPKRDATEMYLKSKALLEKPYTKDIDVFLKRDIETGFGFRVLGGEGPQQPVYIGAIVPSGAAEKDGRLRAGDELIGIDGVMVKGRSHKQVLDLMTNAARNGQVMLTVRRKVIYRDEDIQEMTPVLVNGSPKLPRLPMPSALDHESFDITLHRKENEGFGFVILTSKSKPPYGVIPHKIGRIIEGSPTDRCGLLHVGDRISAVNGRSIIELSHNDIVQLIKEAGTVVTLTVVPEDGEYLLSTEKKPLIIIPAFCYHNVKDKRDVVNWSDHKTLPPGEQGTLCVTGPNQGCLTVELERGPRGFGFSLRGGTEYNMGLYILRLAEDGPAQLDGRIHVGDEIVEINGEPARGISHTRAIDLIQAGGNKVVLLLRPGAGLPRHSRDLGSLEESDGWAEKNINRLSPLLYDQLKFSKRTRSITDSRELSSPKKTEETFPKAKEQHQSPKITVSQSEEAKPSRSKMPQMNNTKIQTVNHEMARFRPQLVSNLQQMENQQGHHRRIKDPFSRESLGNENKNDEKGTPKIRRYTIGNEKRTRPGLEQESFKRSEGEFLRHRTQSVTVRESQTSRSKDSELRQVNGNPAENKTNSKDTGNKRDSLSFFKDIWSRKDYKTSPQETKGRDGKLSNFKERRKKEDVVASTDSIPKTTQSQKGTVGPGPWKVPSSAKILTKAEVLRDPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPJ4</accession>
    <name>A0A3B5MPJ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Collagen, type XIV, alpha 1b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSXCOT00000026017.1">
        <property type="protein sequence ID" value="ENSXCOP00000025708.1"/>
        <property type="gene ID" value="ENSXCOG00000019206.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153769"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005614">
        <property type="term" value="C:interstitial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd01482">
        <property type="entry name" value="vWA_collagen_alphaI-XII-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000489">
        <property type="entry name" value="collagen alpha-1(XII) chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000444">
        <property type="entry name" value="Collagen alpha-1(XIV) chain isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000008">
        <property type="entry name" value="Collagen type XII alpha 1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000234">
        <property type="entry name" value="Collagen, type XII, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Collagen, type XII, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000001">
        <property type="entry name" value="Collagen, type XII, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048287">
        <property type="entry name" value="TSPN-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF15">
        <property type="entry name" value="COLLAGEN ALPHA-1(XIV) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00210">
        <property type="entry name" value="TSPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="27"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="12">
        <location>
            <begin position="153"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="421"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="507"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="598"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="702"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="794"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="12">
        <location>
            <begin position="1012"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="101"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1409"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1501"/>
            <end position="1624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1642"/>
            <end position="1672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1689"/>
            <end position="1714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="111"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1524"/>
            <end position="1554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1608"/>
            <end position="1618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1714" mass="187153" checksum="7FD4069103CCADA8" modified="2018-12-05" version="1">MYILTRCVRLFRPVNMIYFPLSVTVSSPRRFRVKVLGQDKLEVSWKEPKGDFEGYKVIYITRPGGQQKVLELAKQETKLIIKDYNPSKDYNFKISAVNGGKESKPLLGKQEAQQSGPETAQTQGTKKSDVTRENNEISEAEDGFMCKTPAIADIVILVDGSWSIGRINFRLVRAFLENLVNAFSVEFDKTRIGLAQYSGDPRIEWHLNAHTTKEAVIEAVKNLPYKGGNTLTGLALTFILENSFKVESGSRPGVPKIGILITDGKSQDDVIPPAQSLKDAGIELFAIGVKNADENELKAIASPPEETHVYNVADFSVMSDIVEGLTKTVCDRVEQLDKKIKGSTRLINPSFSISKHDWKAVFGDPDPDFKAVFQVVVGTENSVELTYLNSLTEYQVAVFAVYRSSASEALRGSATTLALPTVNNLELFDITHSTMRVRWKIAAGASGYMILYAPLTEQEVKVGDSVNEVELEGLTPATEYTVTVYAMYGEEASDPMTSQQTTLPLIPASNLRFQDVDHSSVRITWDSPSKLVRGYRIMYVKTSGVQTTEVDLGAVTSYLLKNLTSLTEYTVGVFAVYKEGEAPAVTESFTTKMVPDPMDLKSSDISSDGFRLSWQHAASDVVLYRLTLTPTDGGESKEVLVNGNINTYLTKGLEPASEYEVLLAAIYANQEESDEVVLIETTAKRTTTVATTTTTTLTPRYAVKNLKIDEETTFSMRVSWQAVDSRNVRHYQLTYISARGDRAQEMRTVPSGQTSLVLQPLLSDTEYKVTVIPVYNEGDGPVSSQMGRTLPLSAPRNLRVSEEWYSRFRVSWDVPPSPTMGYRVVYQPLSAPGQALETFVGEDVNTMLILNLLSGTEYSVKVIASYTTGSSEALTGKAKTLYLGVTSLSTYQVRMSGVCAQWVPHRHASTYRLVIQSVTGSQKQETKLGGGASRHCFSSLKPNTEYKISIYSQLQDGTEGPAVTANVKTLPIPTLAPAKPPTTSPPPTIPPAKEGETSCHSFCSTCLLPLDRVAIAQFSDDARTEFKLNSYGDKERLLDAINKISYKGGNTKTGRAIQHVKENIFTAEGGARRGIPNVLVVLTDGRSQDDVNKVSKEMQMSGYIVFAIGFADADYGELVSIASKPSDRHVFFVDDLDAFQRIEEKLVTFVCEAATATCPSIPMSGSTTPGFRMMELFGLVENKYNSISGVSMVPGTFNAFPCFHLHSNALMAQPTRFIHPEGLPSDYTITLLFRVLPDTPEEPFAIWEILNKNNDPLTGVILDNGGKTLTFFNNDFRGDFQTVTFEDPEIKKLFFGSFHKLHIAISKTSAKVFIDCKMVAEKFINAAGNITTDGVEVLGRMVRSRENKDNSAPFQLQNFDIICSTSWANRDKCCELPGLVRSNFKAAELDLNSFTFIFFFYQGPAGPVGDTGVPGPQGPPGPQGPSGRSIIGPPVRTSGFLIYILQFLCDQIHFQYLDMLSAGFCRREGGDLQSTASVQAIARQVCEQLIQSHMSRYNTILNQAPSPPVSIRTVPGPPGEPGREGAPGPQGEQGPPGRPGFPGQNGQNGNPGERGQPGEKGEKGSAGVGVQGPRGPPGPPGPQGLGRPGSQGPTGRPGNPGTSGRPGVPGPVGPPGPPGYCDQNSCLGYNVGVQLPSSVFQNYGEAEEDDPYRYYQPNYPAPQPVSPDDPALALDDVELRSPGVYRSKRKAEEVQAGTKRRIKRGVKNLPKLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQY2</accession>
    <name>A0A3B5MQY2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQY2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026824"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027153.1">
        <property type="protein sequence ID" value="ENSXCOP00000026824.1"/>
        <property type="gene ID" value="ENSXCOG00000020030.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160562"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF125">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE_PHOSPHODIESTERASE FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="416"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="466" mass="52943" checksum="FF92D70DFFE3C4B1" modified="2018-12-05" version="1">INVCLSRFSVCVLQVERPKLLLVSFDGFRWDYIYRVPTPNFRSIMDEGVKVEFVENIYITKTYPNHYSMVTGLYAETHGVVANEMYDPALNLSFSMETDSVYDSRWWEQAVPLWVTVQKDGGRSGAAMWPGSDVLIHGVFPTRYLPYNASVSFEARVRRIIEWFSAPEGEAVDFGILYWEEPDESGHNLGPESPLMDSIIAGIDEKLGFLLDELKKAGLYEKVNLVVTSDHGMTQLSPEKIIELDQYVDRDLYTWVDKSPVVGILPKEGKLDEVYAMLADANPNMVAYRKDDIPEHFHYQHNVRIMPILLEAKEGWTIMQNRTGLFMLGNHGYNNTLRDMQPVFVARGPAFRQNYIKASMRSVDLYPLMCHILAIRPLPNNGSLLNVRDLLSSEPTTSVPASAHSTPSPKASRYSYAPVVGSFIGVVMVLGFLVVYVILVTLKQRPVLKHRSWEMSQPLLQEDLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MR12</accession>
    <name>A0A3B5MR12_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Discs, large (Drosophila) homolog-associated protein 4b</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MR12"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023631"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023914.1">
        <property type="protein sequence ID" value="ENSXCOP00000023631.1"/>
        <property type="gene ID" value="ENSXCOG00000017652.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155308"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099572">
        <property type="term" value="C:postsynaptic specialization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005026">
        <property type="entry name" value="SAPAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12353:SF19">
        <property type="entry name" value="DISKS LARGE-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12353">
        <property type="entry name" value="DISKS LARGE-ASSOCIATED PROTEIN DAP SAP90/PSD-95-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03359">
        <property type="entry name" value="GKAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="61"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="267"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="413"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="468"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="657"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="860"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="468"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="491"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="538"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="657"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="690"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="725"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="880"/>
            <end position="890"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="933" mass="101232" checksum="51E83FB8A565F2E5" modified="2018-12-05" version="1">PPSLLDFEKQLPGGRDGFSTLQFHRTSAAVAAKQRTDSPGRIRYMLHSVQKLFAKSQSLESHNMKGNVNGRSTGSGGGTSSGTEDGGKHTRRSKSKDRITKSEATAKRRPRSNMSGYWSSDDLDSSDLSSYHNTMTMMTLGRPSGHESHSGQSKYIHSGYNTISSSKSSNDMKYPALPGPGDIGGGGGISGPVGMLMNDTEYVKGGSWSTLTMGQPRQVLQKGSTTLDRSMLKSKSCQQELTCNYLQVGRREIPCRRMRSGSYVKAMGDMEDSDDSEGSPKPSPKSAARRQSYLRATQQSLSDQLPPRCLPSLRELSNNRSLDNLDCIGGPGALAPWDEDSFSQACSTLGRRSGIEQLRDLEKSHHYEDRSSVSAFRDIHSQDISEPPDMPMPTCFRSRSHSYLRAIQAGCSQDDDTASIDSGCSPPPTDSSVRTYSTSTSTCITSCKKAAPPPVPPRTTSKPYISVTVQSSTESAQDNYLDQQDRRSEVNSQSSHAHSNSSDSLDSTHANSLARGIPRPPHIIPIPIATPREPIIPSTANASTETSDSAVHNESLKSGSSKGNLAAEEPFVAPLPRRKLSSIGIQVDCIQEVPREETPPLARFQSIGVQVEDGWLSRSSSMASKQETDSDTQDISAISHISIAKTFEKKVMVNSASQSMSSLPGQDSLDNGDQTGNTSSPPPPRQILNRSTTRSSSSSFSESLDPALDPSSLPPPDPWLDSGNGSSGGVSQSGGGGTLCRRDGHWFLKLLQAETARMEGWCQQMEQETKDNQLSEELGKVRSAVGSAQLLMSQKFQQFRGLCEQNLNVTANPRPTAQDLAGFWDLLQLSIEDISLKFDELYHLKSNDWQPLQNEDKEALLASKKPSKTRPTLAREKSADSSSTSSSASAEKQRQEARKRLLAAKKAASFRQNSATESADSIEIYVPEAQTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUU6</accession>
    <name>A0A3B5MUU6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prospero domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MUU6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027639.1">
        <property type="protein sequence ID" value="ENSXCOP00000027306.1"/>
        <property type="gene ID" value="ENSXCOG00000020395.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154790"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="18897" checksum="1DCA38DFB0657D50" modified="2018-12-05" version="1">MPDHDSTTLLTRQTKRRRVDIGVKRTVSSAIFPRARETLLEAMNQSHGPDQDGDLSLVRHGHGDGEKTNVLRKLLKRANSYEDVMMPFPGATIISHLLKNNLGKNGGSDSGFQSELTLSSGGSEIQAEDACSNSSRDSPSDCLSPGPKDQPMCSLFFLCSETIAPTSREVLPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVF9</accession>
    <name>A0A3B5MVF9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVF9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027551"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027885.1">
        <property type="protein sequence ID" value="ENSXCOP00000027551.1"/>
        <property type="gene ID" value="ENSXCOG00000020559.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183152"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089701">
        <property type="term" value="C:U2AF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009145">
        <property type="entry name" value="U2AF_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12620">
        <property type="entry name" value="U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01848">
        <property type="entry name" value="U2AUXFACTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="150"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="195" mass="23404" checksum="7FF41C4BD258E0F9" modified="2018-12-05" version="1">MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQTILIQNIYRNPQNSAQTADASRCAVSDVEMQEHYDEFFEFRREEDAEKAVMDLNNRWFNAQPIHAELSPVTDFREACCRQYEMGECTRGGFCNFMHLKPISRELRRELYGRRRKKHRSRSRSRERRSRSKDRRRGDRERRKSRDRERSGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVQ8</accession>
    <name>A0A3B5MVQ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ABM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVQ8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027676"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028011.1">
        <property type="protein sequence ID" value="ENSXCOP00000027676.1"/>
        <property type="gene ID" value="ENSXCOG00000020635.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183131"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042984">
        <property type="term" value="P:regulation of amyloid precursor protein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000028">
        <property type="entry name" value="N-terminal EF-hand calcium-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039862">
        <property type="entry name" value="NECAB1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12178">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12178:SF2">
        <property type="entry name" value="N-TERMINAL EF-HAND CALCIUM-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51725">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="2">
        <location>
            <begin position="154"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="254" mass="28825" checksum="C3100650D8F44ECC" modified="2018-12-05" version="1">MFLDYLLLASKFTHLIFFVSSQVYERGTNVEQFVTRFLLKESANQIQSLLSSVESAVDAIDEQHGQSGHQPAKVSPRMPEKRYQNTVPDYPPNNRVSAREAVRTINTASEVRKEGLEAQINRLAELIGRLENKTVSFDLHQRLTDTDGTPSSLLLIRQEMVVSQKKLGEFCEALKQYLKNVSAQRDCFHVTAVRLPDGLSFVVYEFWDGEEEWKRHLQSAPNKAFQHVKVDTLCQPEAISSVAVPAAWCSVNRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MW06</accession>
    <name>A0A3B5MW06_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>17beta-estradiol 17-O-(beta-D-glucuronate)(in) + ATP + H2O = 17beta-estradiol 17-O-(beta-D-glucuronate)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60128"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:82961"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:60129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an S-substituted glutathione(in) + ATP + H2O = an S-substituted glutathione(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19121"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.6.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:19122"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>leukotriene C4(in) + ATP + H2O = leukotriene C4(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38963"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57973"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:38964"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MW06"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025522"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025830.1">
        <property type="protein sequence ID" value="ENSXCOP00000025522.1"/>
        <property type="gene ID" value="ENSXCOG00000019073.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153931"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015431">
        <property type="term" value="F:ABC-type glutathione S-conjugate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000163">
        <property type="entry name" value="Multidrug resistance-associated protein member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF357">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="35"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="299" mass="33430" checksum="C214E5BA978F625C" modified="2018-12-05" version="1">MTSVERVVEYAELESEAPWETDKQPPGDWPQKGSIIFDSVSFSYSDSSPLVLKNLSFVFTSREKVGIVGRTGAGKSSLISAMFRLGEPEGRITIDGILTSEIGLHTLRQKMSIIPQDPVLFTGTMRKNLDPFKQHTDEDLWNALQEVLQPSLLTTMVEELPNKLETVLTESGSNFSVGQRQLVCLARAILRENRILIVDEATANVDPRTDSLIQQTIQNRFQECTILTIAHRLNTIIDCDRILVLDAGRIQEYDKPYVLLQNQDGLFYQMVQKTGKAEAASLLQVAKQVSASVPEQVSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWD2</accession>
    <name>A0A3B5MWD2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox protein Hox-D4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Antp homeobox family. Deformed subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027879763.1">
        <property type="nucleotide sequence ID" value="XM_028023962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWD2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025712"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026021.1">
        <property type="protein sequence ID" value="ENSXCOP00000025712.1"/>
        <property type="gene ID" value="ENSXCOG00000019214.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114148581"/>
    <dbReference type="KEGG" id="xco:114148581"/>
    <dbReference type="CTD" id="30329"/>
    <dbReference type="GeneTree" id="ENSGT00940000157270"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060216">
        <property type="term" value="P:definitive hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060217">
        <property type="term" value="P:hemangioblast cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001570">
        <property type="term" value="P:vasculogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000029">
        <property type="entry name" value="Homeobox protein Hox-D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050609">
        <property type="entry name" value="Antp_homeobox_Deformed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017995">
        <property type="entry name" value="Homeobox_antennapedia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45771:SF5">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45771">
        <property type="entry name" value="HOMEOTIC PROTEIN DEFORMED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00025">
        <property type="entry name" value="ANTENNAPEDIA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="141"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="143"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="201"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="217"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="232" mass="26599" checksum="DD97FA0FE817E11D" modified="2018-12-05" version="1">MAMSSYMVNSKYVDPKFPPCEEYSQNNYIPEQGSDFYSPAQDADFQHPGILYPRSNYTEQPFSCSTVPDSAVQPRGHAGHTAHFDAQTEQGPPVQTAGARTCGQQQNAKSQNGLQAKQPAVVYPWMKKVHVTTVNPDYTGSEPKRSRTAYTRQQVLELEKEFHFNRYLTRRRRIEIAHTLCLSERQIKIWFQNRRMKWKKDHKLPNTKGRSAPASGHLQNIQKDNQTDITAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MY50</accession>
    <name>A0A3B5MY50_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dynamin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MY50"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026767"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027096.1">
        <property type="protein sequence ID" value="ENSXCOP00000026767.1"/>
        <property type="gene ID" value="ENSXCOG00000019995.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013727"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008053">
        <property type="term" value="P:mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051646">
        <property type="term" value="P:mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09912">
        <property type="entry name" value="DLP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002843">
        <property type="entry name" value="Mitofusin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027094">
        <property type="entry name" value="Mitofusin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465:SF2">
        <property type="entry name" value="MITOFUSIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10465">
        <property type="entry name" value="TRANSMEMBRANE GTPASE FZO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="7">
        <location>
            <begin position="64"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="300" mass="33428" checksum="470AA995EF1EC260" modified="2018-12-05" version="1">MTTLDSLNLSLVSVWTFRTAHVGLSTVIPEAVQNQDLGPVAVEEQNLEMQSCATKLAAIREVLLRRTMKVAFFGRTSNGKSTVINAMLRERVLPSGIGHTTNCFLSVEGTDAAEPYLTTEASAERRTVNQLAHALHMDPALDSGSLVRVFWPKNRCALLRDDLVLMDSPGTDVTLELDSWIDKFCLDADVFVLVGNAESTLMNTEKLFFHKVSERISKPNVFILHNRWDASVTEPELIDQVRSRYHALAWLTVTDRPAGGALAEGFQDRLTEFQQFERTFELLLLPLLGTANRHVNVTSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MYJ5</accession>
    <name>A0A3B5MYJ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glycogen synthase kinase-3 beta</fullName>
            <ecNumber evidence="5">2.7.11.26</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[tau protein] + ATP = O-phospho-L-seryl-[tau protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12801"/>
            <dbReference type="Rhea" id="RHEA-COMP:13701"/>
            <dbReference type="Rhea" id="RHEA-COMP:13702"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-threonyl-[tau protein] + ATP = O-phospho-L-threonyl-[tau protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53904"/>
            <dbReference type="Rhea" id="RHEA-COMP:13703"/>
            <dbReference type="Rhea" id="RHEA-COMP:13704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.26"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.26" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MYJ5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025405"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025712.1">
        <property type="protein sequence ID" value="ENSXCOP00000025405.1"/>
        <property type="gene ID" value="ENSXCOG00000018974.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00520000055635"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030877">
        <property type="term" value="C:beta-catenin destruction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003146">
        <property type="term" value="P:heart jogging"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001947">
        <property type="term" value="P:heart looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003170">
        <property type="term" value="P:heart valve development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010975">
        <property type="term" value="P:regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000055">
        <property type="entry name" value="Glycogen synthase kinase-3 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000009">
        <property type="entry name" value="Glycogen synthase kinase-3 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF8">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17 24 25" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="19" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="16" id="KW-0221">Differentiation</keyword>
    <keyword evidence="15" id="KW-0418">Kinase</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="14 24 25" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 25" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="56"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="423" mass="47585" checksum="1716EB776D3FE86A" modified="2018-12-05" version="1">MSGRPRTTSFAESCKPVPQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGDKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPMVYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPETAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNIKLPNGREKPSLFNFTTQELSSNPSLASILIPAHARNQAVASTPTNASATTGQSRTSFSRLSSPLSTWKHSLPFLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N009</accession>
    <name>A0A3B5N009_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N009"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026290"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026608.1">
        <property type="protein sequence ID" value="ENSXCOP00000026290.1"/>
        <property type="gene ID" value="ENSXCOG00000019633.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153776"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000103">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="52"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="397" mass="44788" checksum="ED6862E584536CCA" modified="2018-12-05" version="1">MTLTQERPLTYAKMKGLVTFISGRSSFLIINTFLATYHCFLFVFIRVKPSDFDYLKVIGTGSFGKVLLARHRKEGGYYAVKVLDKQMIIKRKEQRRVMVERSVLLKGLKHPFLVGLHFSFQTPKTLYFVLDFANGGELFYHLQREGSFPEPRAAFYAAEIAAALGYLHSLSIVYRDLKPENILLDSEGHVMLTDFGLCKEGVAVGGITHTFCGTPEYLAPEVLLGQPYSLAVDWWGLGAVLFEMLSGLPPFYSCSRLEMLENILYSPLRLPSGLSEGAHSLLKGLLERNISKRLGQRLDIEELKEHQFFASINWDDLVARKVRPLFIPKVVRINQKMVRNIISTSSRLVLIVLSFSRPVLVMCATSLLRSHCNLSLPPSTTGRRQPLPAQPFLDSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N0C2</accession>
    <name>A0A3B5N0C2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CEP170 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CEP170 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N0C2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026500"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026821.1">
        <property type="protein sequence ID" value="ENSXCOP00000026500.1"/>
        <property type="gene ID" value="ENSXCOG00000019784.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157058"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051176">
        <property type="entry name" value="Cent_Immune-Sig_Mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029300">
        <property type="entry name" value="CEP170_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 170 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715:SF18">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 170 KDA PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15308">
        <property type="entry name" value="CEP170_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CEP170 C-terminal" evidence="3">
        <location>
            <begin position="576"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="79"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="203"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="318"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="414"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="463"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="529"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1037"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="532"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="620"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="652"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="672"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="713"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="761"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="805"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="881"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="970"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="994"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1049"/>
            <end position="1058"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1211" mass="131937" checksum="08A73FEED9FC29EF" modified="2018-12-05" version="1">VPEEALKVRSHFNLLAVSLCSRPTPLYGQPSWWGEEDYGSKVQSSDEPHSALSDSQPKTVFPSYHREPSYFEIPTKDFQHQKTSEAELHEIPTKDTDAPNAPPTPTPPVVQSHASFTIEFDDCMPGKIKIKDHVTKFSTRQRKGQAPPAKTAATATPAEMMSAQSKVADWLVHSDVSMMRRHPPCEDVYSTKSDLAMNIKTLKGHHHEDGTQSDSEDPVLKAKSKSQHSPPSPTEMSRQGLSEHLTHQGHSGPQRSSSLKREKTEGEAGSSSSSSRSSSGIIVRPFGSVGKKSKLAQEFATEFLKDSASKSFKPAIGPAQVAPLGHPSGPHMSPMLSLGVHGGDPKISQRIVRNEEDDSLSDAGTYTIETESQDKEVEEARNMIDQVQIHAADLEGPKWVSRWASLADSYAEPVGSYSYDTSESETSHSSRTRRLLPQVPPEKVESVTPSILIRVYVPPCSQDSTQRLTVQDDVDPDSLSDASRSEDGPALVNAKKIDVITGNVSPVAPGYQFKVQEKVSPTNKSTFFYIGAEDHPGKPEQARSPVQGERTRDPPAKTPPTTVLIRHLSGHEPRRTDSVPTKDSCIVRQESFTKDQPSDTVQMKKLPHISSHPSIRDMEQSIQETQPFLQETEGALSSLDTKVPSSGSGRSSKKEGSSTHMDDSLSGESDVDTASTVSQVSSKNAPVSSTSKKRPAISSFQKEKSSSSPSIQEKGRQLSARERLSEKRRTQTTDVPSKAEATKRFQMRRSTGNRGSLDLSEGQQGTAPNWTETTSSDHEISHPSSRTKKVIAPLQKEDNGKTPKSAAQQVLTRSNSLSAPRPTRASMLRRARLGDASDNEGTETDRTSQNSDHISAPPKVSAEGKKLSRLDILAMPRKRTGSFTTPSDNESSTSRSGFSGRNSEPAVTARKTSVGDARQAASKGGGAPLTPFADYFSGSRRKQKGSDFSSSSEEEYDTNTGFSKAKRSSHSTTAGQSQRSQRTAASRTKSVSLETEEDEDQNDIDPYQNWSTHSAEIAKLSQDLAKDLAILAKEIHDVAGDGDPPPTATSPSSLPNTPGSNISAREEVGIKHPSQHGHYHHTTKTLQFRCSTEALVRSCRKLFVCLQVDLQELHSASDSYNLSYVCSSAKTPCVAYLHGVRPAQVSNSDSSPTTDPNTHLHCVGLFCTLELLSCHLVLCVQSTKLESVCWMNIQFSLRLNTSNRGCLCQIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N1F5</accession>
    <name>A0A3B5N1F5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Inositol 1,4,5-trisphosphate receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Inositol 1,4,5-trisphosphate-gated calcium channel that upon inositol 1,4,5-trisphosphate binding transports calcium from the endoplasmic reticulum lumen to cytoplasm. Exists in two states; a long-lived closed state where the channel is essentially 'parked' with only very rare visits to an open state and that ligands facilitate the transition from the 'parked' state into a 'drive' mode represented by periods of bursting activity.</text>
    </comment>
    <comment type="function">
        <text evidence="21">Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="20">Homotetramer. Interacts with CABP1. Interacts with BOK; regulates ITPR2 expression. Interacts with BCL2L10. Interacts with TRPC4. Interacts with CHGA and CHGB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 21">Endoplasmic reticulum membrane</location>
            <topology evidence="1 21">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="21">The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the InsP3 receptor family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000027460"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027793.1">
        <property type="protein sequence ID" value="ENSXCOP00000027460.1"/>
        <property type="gene ID" value="ENSXCOG00000020503.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156039"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070679">
        <property type="term" value="F:inositol 1,4,5 trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005220">
        <property type="term" value="F:inositol 1,4,5-trisphosphate-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000002">
        <property type="entry name" value="Inositol 1,4,5-trisphosphate receptor type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000005">
        <property type="entry name" value="Inositol 1,4,5-trisphosphate receptor type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.30:FF:000001">
        <property type="entry name" value="Inositol 1,4,5-trisphosphate receptor, type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.30">
        <property type="entry name" value="IP3 receptor type 1 binding core, RIH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014821">
        <property type="entry name" value="Ins145_P3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000493">
        <property type="entry name" value="InsP3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013662">
        <property type="entry name" value="RIH_assoc-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000699">
        <property type="entry name" value="RIH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035910">
        <property type="entry name" value="RyR/IP3R_RIH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816:SF3">
        <property type="entry name" value="INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816">
        <property type="entry name" value="MIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08709">
        <property type="entry name" value="Ins145_P3_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08454">
        <property type="entry name" value="RIH_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01365">
        <property type="entry name" value="RYDR_ITPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00779">
        <property type="entry name" value="INSP3RECEPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100909">
        <property type="entry name" value="IP3 receptor type 1 binding core, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 21" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 21" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="5 21" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="22" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="17" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9 21" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16 21" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 21" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 21" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 21" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 21" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MIR" evidence="23">
        <location>
            <begin position="113"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="23">
        <location>
            <begin position="232"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="2491"/>
            <end position="2539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00059076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00061937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU368044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="2559" mass="294317" checksum="F279F88F842FCBC2" modified="2018-12-05" version="1">MSEHRSSFLHIGDIVSLYAEGSVNGFISTLGLVDDRCVVQPDAGDLTNPPKKFRDCLFKVCPMNRYSAQKQFWKAKQGKQGNNNTQAELFKKLQHAAELEQKQNDTENKKLLGDVVKYSSVIQLLHIKSNKYLTVNKRLPALLEKNAMRVSLDPAGNEGSWFYIQPFWKLRSEGDNVVVGDKVVLMPVNAGQPLHASNIELLDNRGCKEVNALNCNTSWKVTLFMKFSDYREDVLKGGDVVRLFHAEQEKFLTGDEYKKQQHVFLRTTLRQSATSATSSKALWEIEVVHYDPCRGGAGQWNSLFRFKHLATGNYLAAEMNPEFKNGTTDAGFSNRKHQAAERIAYTLVSVPHGNDIASLFELDATTLQRADCLVPRNSYVRLRHLCTNTWVTSTNIAIDTEEERPVMLKIGTCSTKEDKEAFAIVSVPLSEVRDLDFANDANQVLESTVRKLKFANITQNERRFVTTLLEDLIFFVCVVPNNGQDVLSVVTSTPNRERQKLMREQNILSQIFGILTAPFKDNGDGPMLRLEELGDQRYSHFKYMFRLCYRVLRHSQQDYRKNQEYIATKFPIMQSQMGYEILAEDTITALLHNNRKLLEKHITAREIETFVSLLRRNREPRFLDYLSDLCASNKTAIPVTQELIIKFMINPINADILIQTKLISNSENSMDSSFSPDDGDEEEVWLYWIDIHKEPHGKSIRHLAQDAKNNHKIDADLLTYYRCQLNLFAKMCLDRQYLAINQINDKLPVDLILRCMFDDCLPYNLRASFCRLMLHMHVDCEPQEAVVPVRYARLWTEIPSKISIKEFKYELTVDSREEMKKKFKSTMEFVDDYLKDVFNQQYPFGDKEKNELTLEVVNLARHLVYFGFYNFSELLRLTRTLLAILDIVQQPFGNNVLRTIHGVGEMMTQMVMSRGVLHGLPDTPPSLRYGIGHFLPDNEDVVVMDTKLKIIEILQFILSVRLDYRITYLLSIYKKEFGESTVPDSSSEPDMDEIAAKAESMFVGSVLELDDEGGRTFLRVLIHLIMQDYPPLVSGSLQLIFKHFSQRAEMLQAFKQVQLLVSEQDVLNYTQIKEDLDKLRLTVEKSELWVEKSGIYGNDELGDAVLSPVQDGENKPQMDSNQENNYEVLKCILERFSRLCCVSKKNRLQLQRLLKNMGAHTVVLELLQIPYEKNDEKMNEIMKLAHSFLQNFCKGNPENQVLLHKHLNLFLTPGLLEAETMRHIFMNNNQLCNEISDRVVHHFVHCIETHGRHVQYLKFLQTIVKCDGKYMKKCQDKVMTELVNAGEDVLVFYNDRSSFSVLLQMMASEQERADENGSLAYHNTLVDLLAACTEGKNVYTELKCNSLLPLDDIVKVITDVNCVPEVKTSYVNFVNHCYVDTEVEMKEIYTSSHIWRLFENFLVDMATVRKLTQKDPLDKYVTETVMAIIRSFFESPFSVNYSMLQVLCSCQTCGSFLLLHGSSISATAKARSIPIPVDLDSQVNALSLNVHTNAVQRAAKDWRTSARSRPRKEPLGSPDYKNIIEKLQGVVSYLEDQFCPRVQAEFSVLVDVLQSPELLFPEAARLRCESGAFMSKLIKHTKKLMEQEEKLCIKILETIREMMEKKESFQESGNELRKILLTRYFGTQKLYSKSEGGNKPCSSMVEIQCLLDKVGATELVIDLIVSTKNDRVFEQSILLGIALLKGGNTQIQNSFYNQLFKQKNSEKFFKVFYDRLRVAQQEIRSTVSVNMFDFSSRKREDDNNFCSTKFRKGKQSNYFLGQNSDAGGADEVEEEPEMSWLIAIMKPVLRYLQLLCENHNSDLQNFLRHQHNKTNYNLVCETLQFLDCICGSTTGGLGLLGLYINENNVDLVRQTLETITEYCQGPCHENQTCIAKHESNGIDIIIALIVNSINPLGENRLDLVLDLKNNASKLLLAIMESRHDSENAEKILYKMRPTELVDVMKEAYTQGYETEEDMDNAGERIKPRDVGHNIYILAHQVIKKATAVVRLWLIHNKILQQSLRPSLGLDDDKDEALHYYAKHTAQIEIVRHDRSMEQIVFPVPNICEYLTEESKNRVFTTTERDDQGSKVNDFFQQFDNLYNEMRWQKMIRKNKALFWFSRHFSLWGSISFYLACVIVDLYVLFIFFCPGTLSPFWNIFLWVALGISTALLPVLPKQWGIVSFLVTLILRAIYTMGLTPALFLLGSINLFNKLVFIVSFVGNKGTFARGYKAVVMDLFFIYHLGYALICVLGLFAHEFFYSFLLFDLVFREETLLNVIKSVTRNGRSIILTAILALFLVYFFSIIGFIFFKNDFRMEVDHLHTSSMLYKISGATCFDNTGDCVGVLMNKDDEVERVCDTLRMCIITVLNQGLRNGGGVGDILRRPSKEEPLFAARVIYDLLFFFIVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTSCFICGLERDKFDNKTVSFEEHIKSEHNMWHYLYFLVLVRVKDPTEYTGPESYVAQMIAEKNLDWFPRMRAMSLVSNEGDNEQNEIRSLQEKLENTVGLVSQLSSQLYELKEQMTEQRKNKQRLGFLGPSQHNHHHIPTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N1J6</accession>
    <name>A0A3B5N1J6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Unc-45 myosin chaperone A</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N1J6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027555"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027889.1">
        <property type="protein sequence ID" value="ENSXCOP00000027555.1"/>
        <property type="gene ID" value="ENSXCOG00000020563.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159320"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000043">
        <property type="entry name" value="Unc-45 myosin chaperone B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024660">
        <property type="entry name" value="UCS_central_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45994">
        <property type="entry name" value="FI21225P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45994:SF3">
        <property type="entry name" value="PROTEIN UNC-45 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11701">
        <property type="entry name" value="UNC45-central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UNC-45/Cro1/She4 central" evidence="3">
        <location>
            <begin position="172"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="811" mass="88883" checksum="99E2AF4B19601009" modified="2018-12-05" version="1">LNSTDARVQQMFSVLLDSSAKDSDRQKAAQNLVVLSRDEGGAEQIFRNDGVKLIQKLLSSKQEDVVLSGLRTLVGLCTGHQSRTMAIVNELGMEQLCSVMGSAASTVSLAACHLLQVMFEALTEGMKREIRGKDEAILPEPSKELRSMLRHLLEMIPAQNVSGPGRDSAINLLVKQVPRKSLKNPDNSLSLWVIDQGLKKILQVAGTVAELSEGPPLTENTHMSCSVLLSKLYDDLKSDKERENFNKLCEEYVQEHFRRSGLDGKLRAIQTVSVLLQGPSDVGNITLEMSGMMDAVISLCASEDVSHQQVAVEALIHAAGKAKRASFITSNGVALLKDLYKKSENDRIRVRALVGLCKLGSAGGTDFSMKQFAEGSTLKLAKQCRKWLCNESLPPTSRRWSVEGLAYLTFDADVKEDLVEDKNALLAMFDLAKSEDKTVLFAVGSTLVNCTNSYDVEKPDPQLVELAKYAKQHVPEEHPKDAPSFVEKRLGKLLEAGVVSALVCMVKQESPALTEACREFIARVFLALVDRQEDRGTVVAQGGGKALIPLATDNTDVGKVKAAQALAKIAITSNPEIAFPGERIYEVVRPFVNLLQLECTLLQNFESLMALTNLAGISERLRQKIIKEKALPKIEGYMFEENELVRAAATECMCNLVLSTEVQKLFVATGNDRLKLLVLYCGEEDERLRKAAAGTLAMLTAEQPELCPRIPGTTTHWLEIFQALLLSEISDLRHRGVVIVQNMMQAEKGLAETLMESEALEILTVLAKGGEGAVGPVSKVAQNCLNKAVEYGIIRNREDRIEKEKGKGTEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KP98</accession>
    <name>A0A3B5KP98_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KP98"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000292"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000299.1">
        <property type="protein sequence ID" value="ENSXCOP00000000292.1"/>
        <property type="gene ID" value="ENSXCOG00000000271.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183047"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd11619">
        <property type="entry name" value="HR1_CIP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000017">
        <property type="entry name" value="Formin-binding protein 1-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057870">
        <property type="entry name" value="HR1_TOCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF22">
        <property type="entry name" value="FORMIN-BINDING PROTEIN 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25610">
        <property type="entry name" value="HR1_TOCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="310"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="180"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="378" mass="43082" checksum="61837D0849EAC205" modified="2018-12-05" version="1">VYTRRHLVAFTSQFNKLSSYNLSSNLSNTKLLWQRDLRKEKWLNVLSVIDSVWSLQRIQDMEERRIERIAEAMRSAAESERKVLPAASRSLDAMMDAAESIQPRKDTGHVVEVYKSGFDPPGDVEFEDYSANMRRSISESSYLDNRAEGQKHSRKLWPFLRKNKGSSVTDGSQLPPEQRRKKLLARINSINQEIQREREDALLKMREVYERSPQMGDARSLEPRLEEVKLNLQRLEEELRRSQVALPAERGDRNPLSTDPLCLGSGPAQAELCSPFSPDGSYTEDQAAEITFKSRSSEFDDDFDDEEPLPSIGTCKSLYPFQGTNEGTLSMVEGELLSVVEEDKGDGWTRVRRNLEEEGYVPTSYIKVFLDGSAKGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQH1</accession>
    <name>A0A3B5KQH1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQH1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000120"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000121.1">
        <property type="protein sequence ID" value="ENSXCOP00000000120.1"/>
        <property type="gene ID" value="ENSXCOG00000000092.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154862"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050321">
        <property type="term" value="F:tau-protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000003">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF98">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="55"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="187" mass="21109" checksum="8D94BEE3C3EB828D" modified="2018-12-05" version="1">MSTRTPLPTVNERDTEQHAPGEDGRGEALPRPVRSARCKNAAAVTADDAPHVGNYRLLKTIGKGNFAKVKLARHIPTGREVAIKIIDKTQLNPTSLQKLLREVRIMKNLNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKHIVHRDLKVTPFSQPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KSR5</accession>
    <name>A0A3B5KSR5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KSR5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001552.1">
        <property type="protein sequence ID" value="ENSXCOP00000001527.1"/>
        <property type="gene ID" value="ENSXCOG00000001239.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234530"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF127">
        <property type="entry name" value="NITR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="46"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="151"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="294" mass="32913" checksum="160BF19A9A110201" modified="2018-12-05" version="1">MISSYCLHRKHSCVFEIINVNECIFTAFFSSAMASHLISLTLVDIGDNITFDCIVSEKELKFVNWYKQSLGHIVEIVATVILGQTTITEKFKGSHFAIKPEGNRYDLTIFNISKEDEATYFCHIGTTYSQKFISGTFLAVNGKMLFSTELVQQGETVTLQCSLLSKNKEKSQCPTKPSMYWFRTESERFHPGIIYTQRNISDKDLDRSCSYSLSVRDSSDAGIYYSAVVACGSIVIGEGTKVEMRKDSNPLVVGLGVLLAFCVSVIFLLIFNNKDKNICQHLKGRPAIFGDCSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KSX4</accession>
    <name>A0A3B5KSX4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MACPF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complement C6/C7/C8/C9 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KSX4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003245.1">
        <property type="protein sequence ID" value="ENSXCOP00000003208.1"/>
        <property type="gene ID" value="ENSXCOG00000002433.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063725"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022829">
        <property type="term" value="F:wide pore channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001771">
        <property type="term" value="P:immunological synapse formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001913">
        <property type="term" value="P:T cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020863">
        <property type="entry name" value="MACPF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052784">
        <property type="entry name" value="Perforin-1_pore-forming"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096:SF1">
        <property type="entry name" value="PERFORIN 1.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00279">
        <property type="entry name" value="MACPF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017307657" description="MACPF domain-containing protein" evidence="8">
        <location>
            <begin position="24"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="9">
        <location>
            <begin position="27"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="547" mass="61548" checksum="A75000D1175A0C29" modified="2018-12-05" version="1">MLSCLGLAFLLLSLLIVQPQVLSCRVGDQRECDAAPFVPGHNLIGEGFDMVTMQRKGAYVIDMETYLNPNRTCTLCSNQLKGHQLQKLPVSALDWRAFSQTKTYSYGGFHTSVRTATHSAHKRGCSAITGPLRTLLNYSIEFNYQSSFIMFMYIELFAGCLFVPSYRVSTAPPFSSEFLKDLARLPRYYNSSTSSHYKDFLHTYGTHYIHQVRLGGYVARVTAARTCLSTLNGMSSNDSLAFFKTLQVHSCVSMGIEVGLGNFKLSNVPSPCSKFLQNHGFSTVFSSYIHHHYTVISGGNGWSGEFSLTHNDSLGFKNWQHTLKDHPDVVFFFLRPIHLLIPTKTKRVGIKVTATKYLEDNAMESSPREPECTGNTPNLARNCCPQQVLKGTLRVTSIRAWGLSGDYAGATERLANKRLEIFVNITDVINSDYPYWTVDYNFGKVYTLQVLAVEIWDEDLQYDDLLGSCVRYLSQGDNSFTCAAESGRFEVQYTLTCDPYLTGERCGQYKPSPYADTQTFSILCPKYCRGILIFYFLKNPIRPSCLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTQ0</accession>
    <name>A0A3B5KTQ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Interferon-induced GTP-binding protein Mx</fullName>
            <ecNumber evidence="2">3.6.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Interferon-inducible Mx protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.5" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTQ0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003483"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003521.1">
        <property type="protein sequence ID" value="ENSXCOP00000003483.1"/>
        <property type="gene ID" value="ENSXCOG00000002749.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155214"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016185">
        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01256">
        <property type="entry name" value="PH_dynamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000014">
        <property type="entry name" value="Dynamin 2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000010">
        <property type="entry name" value="dynamin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000045">
        <property type="entry name" value="dynamin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="14">
        <location>
            <begin position="28"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="515"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011980"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00015210"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
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            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="638" mass="72576" checksum="C612B806B9CDA5AE" modified="2018-12-05" version="1">MGNRGMEDLIPLVNRMQDAFSAIGQNANLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPLVLQLMNCPTEHAEFLHCKGKKFTDFDEVRQEIEAETDRITGANKGISPVPINLRVYSPHVLNLTLVDLPGMTKVPVGDQPADIEAQIRDMLLQFVTKENCLMLAVSPANSDLANSDALKIAKEVDPQGMRTIGVITKLDLMDEGTDAKDILENKLLPLRRGYIGVVNRSQKDIDGKKDINAAIAAERKFFLTHPAYRHLAERMGTPYLQKVLNQQLTNHIRDTLPGLRAKLQSQLLSIEKEVEEYKNFRPDDPSRKTKALLQMVQQFSVDFEKCIEGSGDQIDTAELSGGARINRIFHERFPFELVKMEFDEKELRKEISYAIKNIHGIRTGLFTPDMAFETIVKRQIGKIKEPCTKCVDMVISELVNTVRQCTKKLAQYPMLREEMERIVTQHIRDRENRTKGQVLLLIDIELSYMNTNHEDFIGFANAQQRINQMNKKKTAGNQVIRKGWLTINNIGIMKGGAKEYWFVLTAESLSWYKDDEEKEKKYMLPVDNLKLRDVEKGFMSSKHIFALFNTEQRNVYKDYRQLELACESQEDVDAWKASFLRAGVYPERVTVSLMSLLTTMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KWH8</accession>
    <name>A0A3B5KWH8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Netrin 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000001976"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002004.1">
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        <property type="gene ID" value="ENSXCOG00000001587.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016358">
        <property type="term" value="P:dendrite development"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:motor neuron axon guidance"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Netrin 5"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF383">
        <property type="entry name" value="NETRIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017467647" evidence="4">
        <location>
            <begin position="30"/>
            <end position="726"/>
        </location>
    </feature>
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        <location>
            <begin position="52"/>
            <end position="264"/>
        </location>
    </feature>
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        <location>
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            <end position="359"/>
        </location>
    </feature>
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            <end position="407"/>
        </location>
    </feature>
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            <end position="621"/>
        </location>
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        </location>
    </feature>
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            <end position="313"/>
        </location>
    </feature>
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            <begin position="350"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="396"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="596"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="655"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="717"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="726" mass="80575" checksum="0C2D42E288F75C53" modified="2018-12-05" version="1">MVRPSSLPPSISLIPVLLRLLLLPPSLISSSFSNAPLSWTSPHEPCYHPDGRPRHCLSEFINAAYGVQVNVTHSLHGPDNNPNITTLTDLHNPNNLTCWRARAGGDSGDWVFTLPLGRRFEITYISLQFCSQGQPSDPISISILKSMDHGRTWRPMQHYSSNCLEDFKQPSLTIAQTRHQETEPLCSDPRPLQRQRGSTVLAFSTLDGRPSSPDFDYSPTLQDWVTATDIRVVFHRTSNNVKATDKMEEIQWRDGLPDSKRTGILKWKLDTKSDTGDQVDKLRDNALAFFDKDKRKNSEISRRNKEERIDKQRQKDHRKGSGHGQDGHNVTSKEGEDTFGTTDLLASPKKGGKARKRNRNKENNFWVPCSNGDCTWTVEGRSRKNGGWELRKRRNNNPNTKQNLRTSQPSGLILPVRAPLAISDLQVGGRCKCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXHSGGRSNPCVPTSVQHRAEGAGERDGAFRTVVAVLHRCTNRLPDRLLPCPEGLPVPLGSRPRPQLRLPRPPRGPDIPPHRRRGRGAELGSGRESPGGGSLRTGPPVAGTLEPQAQGLPGAGQEGPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXP2</accession>
    <name>A0A3B5KXP2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXP2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004932.1">
        <property type="protein sequence ID" value="ENSXCOP00000004873.1"/>
        <property type="gene ID" value="ENSXCOG00000003821.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153713"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024836">
        <property type="entry name" value="JAKMIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18935">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18935:SF7">
        <property type="entry name" value="JANUS KINASE AND MICROTUBULE-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="260"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="16"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="206"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="261"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="288" mass="33418" checksum="0B36EC47A308730D" modified="2018-12-05" version="1">MAKKGRTKGEKPEALISALQAANEDLRSKLTDIQIELHQEKCKVSKLERDKVQEVKRVREQEQHRHTAMLTEQRAKWHEEKQKEQQALRENLTRQHEQELARHAKIKDQENQRLKAALNAIRDGSGEKVRTALTLEAREEARRFFDQERVKLLQEIVELKNTKKQTDEALSNMIQADKMKAGDLRVEHQQHQEEISKIKWDCERDVRRLVDEIKSKNRTIFSLEKELESTAGYLQKLQLQKDALDEQLFLVKEAECGLGSPKREIPGRAGDGAEHCGSPVNYRDSTGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZK9</accession>
    <name>A0A3B5KZK9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Nephronophthisis 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZK9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003485"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003523.1">
        <property type="protein sequence ID" value="ENSXCOP00000003485.1"/>
        <property type="gene ID" value="ENSXCOG00000002751.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048827"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097730">
        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904491">
        <property type="term" value="P:protein localization to ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029775">
        <property type="entry name" value="NPHP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31043">
        <property type="entry name" value="NEPHROCYSTIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31043:SF3">
        <property type="entry name" value="NEPHROCYSTIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="230" mass="25439" checksum="D88452DE902B8991" modified="2018-12-05" version="1">MADSWEELFHKNRLVPPSSQTVRLAVENHLTANHGFQLSFSRLTVAQLPQVIPNIYVTYQLRVTLYDGKLQHFFGKTWKSSSQKMKNNKISFNEVLYLHTSVLLPSTLVVVELVSLSTKPDGSYLSLGRGFSILELFTNRPEGPGTNGDRRLNLHHGSPRGLLHPSLKDSVDCKNNLVIDGAHLDCVMKTHPSLNSAVHLLPEHALVSGEDNIPGLIPSPTGELFTSGKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZR9</accession>
    <name>A0A3B5KZR9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZR9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003121"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003158.1">
        <property type="protein sequence ID" value="ENSXCOP00000003121.1"/>
        <property type="gene ID" value="ENSXCOG00000002466.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00500000044913"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003011">
        <property type="entry name" value="Cell_cycle_checkpoint_Rad1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003021">
        <property type="entry name" value="Rad1_Rec1_Rad17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10870">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10870:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02144">
        <property type="entry name" value="Rad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01245">
        <property type="entry name" value="RAD1REC1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01246">
        <property type="entry name" value="RAD1REPAIR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017360467" evidence="6">
        <location>
            <begin position="24"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="202" mass="22558" checksum="B7A932D9B60DD7B8" modified="2018-12-05" version="1">MVIFQVNLTVLLDCLNIFGGSTAAGASTALRMCYMGYGHPLTLFLEEGGVVTVCKINTQEPEEPIDFEFSSSNVTNKVILQSESLKEAFSELDMTSEVLQITMSPSQPYFRLSTFGNSGNAHYDYSKDSDMMELFQCSTTQTNRYKMSLLKPSTKALAISCKVSVRTDSRGLLSLQYLVRNDDGQICFVEYFCCPDEEVEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZU1</accession>
    <name>A0A3B5KZU1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ERCC4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EME1/MMS4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZU1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004072"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004116.1">
        <property type="protein sequence ID" value="ENSXCOP00000004072.1"/>
        <property type="gene ID" value="ENSXCOG00000003200.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063937"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048476">
        <property type="term" value="C:Holliday junction resolvase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.670:FF:000002">
        <property type="entry name" value="Crossover junction endonuclease EME1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1620.30:FF:000001">
        <property type="entry name" value="Essential meiotic structure-specific endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.670">
        <property type="entry name" value="Crossover junction endonuclease EME1, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1620.30">
        <property type="entry name" value="ERCC4, Mus81-Eme1 complex, nuclease domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042530">
        <property type="entry name" value="EME1/EME2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043086">
        <property type="entry name" value="EME1_nucdom_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033310">
        <property type="entry name" value="Mms4/EME1/EME2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21077:SF7">
        <property type="entry name" value="CROSSOVER JUNCTION ENDONUCLEASE EME1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21077">
        <property type="entry name" value="EME1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21292">
        <property type="entry name" value="EME1-MUS81_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="8">
        <location>
            <begin position="82"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="362" mass="40886" checksum="59DA484733FB1E4F" modified="2018-12-05" version="1">MYSDSPQREDLRSSSDSDEELPVFDFLQPRPSTDVASQKARERQQQDREALKQEQERQKAERRALVEAAKALRPEECIRHMVVAVDPALLQLEGGGTLLASVQALGCSCAIEKQPLPRSVTWARQAPSLDLKRVSPSACVTQEERHSGPACSPTLTSWVREQQRRHPDKRLSLAVVELESYFRRKLGIRFTIFTDPRAKSHQAVVHLQLHTGVSVRFLSTWKEFSDHITMTTKAVAEAPFKRAREQTGFSFYLESDWAGGQRVDKAGKGLLQAWKRQIQQFNRVSSDVAAAILAAYPSPQLLKKAYSQCRTENEKLSLLSDLLIRRGEGVTSTTRRVGPELSKRLFLLMHSRDPEQNLDASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L0A1</accession>
    <name>A0A3B5L0A1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cilia- and flagella-associated protein 45</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CFAP45 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L0A1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001239"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001257.1">
        <property type="protein sequence ID" value="ENSXCOP00000001239.1"/>
        <property type="gene ID" value="ENSXCOG00000000998.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111174"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="539" mass="56538" checksum="DE971D9043D85745" modified="2018-12-05" version="1">MGGKLSKKKKGYDVSDPKDKKDESPASGAGAEESAKVEDGAPPTGAQESAGKDGVVEEIAVSADAAVTEAAVPPEEPKSAHTEEQAPAAEEEAVREQKPEAAPVKEEPAAVAVETTPAVDKPVAVSENKALDGEEKGVSPEIASPVAEIVPEEPKSPPPEEVAATEQKVTAVEVTPVEAVVQAKQAPVEAPAEEAPLAEQEPASPAEKPVAAETAPAVETEKSPVAVEESFPSEEPVKAEAEPEEPPVEEPPVALGSVPQPDAAQVRTEAASEDQQLGPAQETVAEPEAIEVERVSAAAVPEPEPEAEPVAELVSQPEPEQHTLQTTEEESMKAPTPEPEQTTEPESKETTEPEPEQFKEKKQLEEPEPQPTLAAPDTVELETVEAESSEAVVEHASAENHHAEEESASAEAPGSDDPIESQAAEPVPEIVISQSASANSMTVESDKTAETSLEEVPGQEKKSKMENGDLGSPSSTENGVDPVIAVNGECTDNTNVVASHAEEFVNGNEKSEERPLEEQRDFELKKDVNLCGDVQEVPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2N5</accession>
    <name>A0A3B5L2N5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2N5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006658.1">
        <property type="protein sequence ID" value="ENSXCOP00000006578.1"/>
        <property type="gene ID" value="ENSXCOG00000005092.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183102"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="8">
        <location>
            <begin position="75"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="301" mass="33438" checksum="C08BB05C7D4CBEC5" modified="2018-12-05" version="1">MSQPPRSNRGHRERNGEQRRPGDNRRSTPDRWDSILYMRSSSFTASHYSRRESEPPTRYLREDPRMEHQASKCTNICSRRGIVLICSVLTNALVLICVVAAQMVMSGLSSMGGMGGISINSNFNLQGTELQQVRDLDMKYSQMRAPGIYGGIAFSLTFGVVSLLFVVAGNKPAHHLNQKLLVGALVFQAVGAVGYVVAVGLYLHFVIQVNSTDVCKLRQALYSRNGYTWMNCDVSGGDAAVALFGLITAILYTAGTVLTVQTIRGVKRYQQEKKRRHAEKQEGQGNHHRAPLRADITFYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L698</accession>
    <name>A0A3B5L698_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000005930"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005996.1">
        <property type="protein sequence ID" value="ENSXCOP00000005930.1"/>
        <property type="gene ID" value="ENSXCOG00000004597.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182963"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000049">
        <property type="entry name" value="NHS-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024845">
        <property type="entry name" value="NHS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23039">
        <property type="entry name" value="NANCE-HORAN SYNDROME PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23039:SF3">
        <property type="entry name" value="NHS-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15273">
        <property type="entry name" value="NHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="665"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="742"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="836"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1114"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1157"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1228"/>
            <end position="1495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1511"/>
            <end position="1542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="548"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="753"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="863"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="893"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="936"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="968"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="986"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1019"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1115"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1181"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1243"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1280"/>
            <end position="1294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1323"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1333"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1355"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1371"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1411"/>
            <end position="1424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1432"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1483"/>
            <end position="1495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1513"/>
            <end position="1526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1542" mass="166432" checksum="993F700E686DC0CD" modified="2018-12-05" version="1">MPFHQRTIEPRRVSRLSARDGWKPREGSGRRKLRRPVLFSSLDEVGCHTLTSLIQQLSDLSRHASDIFLGIEMEAGMVFRRSCRIQGRLHNLQNIRVLSINFWCVHITFFCQINAKIIAFSSKFHRNIHLGVTFLQPLMFFFIFIFICLCPPECDKLRKDGFRSSQYYSQGPTFSDPKQSTSSLQDDEDASSAEDDKSQLSVRSPTPQGGSEVGEASEVDGQVVWNKAPPLPTPEEKMRQAAQAVPTDVVPINITGAVFDRQASIRRSLINTDTVPRRPKKVKRRKTISGLPDNINQKLGTDLLGSVNSTLRHSETRDSSCQTEEVKIVPPSMRRIRAQRGQGIAAQMAGISASSSTGSISISSSDSSGVVMMPHLFNGDPSRFHSLPRQGARVSLSADPIYSSTPKKPEDQPQRQIGKLQVDDTVVHMRNTPRMNSLPRPKSQEVSGTHPAEWGGSTACVVSPHAAYSTSYIPNATLSSSTEVISLKTSSQSVSAYPISRALSQASSTNTDHLMSSPTAFSQSPSLTTSTPMHTPQDICVTPARPASESGHSDSSIHSCSTLAPTPPSFLPEENWIYDTPENVMTPHRTLTSSCSTPINQLYSSLEHSSRTTTDSSSLYSQDNDGYYTSMHLDLGLRSRSHGSGHGATAGRASRHSMYECREMVGEEDSGSLYSDRSLSRSISLRKSRKPPPPPTRTDSLRRKPATKKPLGGVAAISGANMPNGSMLSETLIASLQQSLQMGLREGKGKGASPSSPSHSPSSDYDDPWVMRSRSQSSISAGSSAASLAASANGAGMPSVYSLCHVTPTPSDTSSLRSDYAESWGYYSDYPRSQVDQRVQTPPGHAADRVTAGGHPGKFRKAGQTQGATSQEVEVSAKSKPSNSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPAFVRSMSPSGGKPRPKVPERKSSLLSSVSVSSSSTSLSSNTSDSLKCSAPTTPLNVPPPFPPPPSSTPPPPQDTSFPAHSTSPEFPPPPSPDMLIHTSSSFNGSLSPPPHPPLPAIGPPPPPPLPAFASVCTSPPKKTAKDPPMADLSNSPSNSPKPLITPFALQSVQLRSVRRPENEINSKSDNIETSLETILGLKHQDQELSYSQGHPTVLTSLTGSSEQDSLKAPPSPVSQLLEELSLDCSFTDETPDGAPINGETDEQSYRMLNGEENKERSSPIKQKPPAVAKKPKVSLILPFSTGEHVQLQLLDATPLTQADDQVDAPHRQIDEEVIEQSEEQEEEDTQESFELSGRGETSPDQDDSCYTNEDRSVDHELANGEVHEEEEEEDGMSSTTGSISSKDDDIGGRLCVHRSKRKVLGRRESEEDKSLTGSQPQSPPSTPTSLSPGTVSPLPRQAGSIQRNLRKTSTSSDSFKALLLKKGSRSETSFRMSAAEMLRSTDPRFQRTRSDGSLDPALGSPTSPITPNSPSNSPSRGKRASELEWGRYEGLSSPTSSPYSMGISKFGRSRTPPSAASSKYNARNRILSSPMTVICEREGELAESENGETAEDLSDPAVRTLPVLTDSNGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6R8</accession>
    <name>A0A3B5L6R8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Direct IAP-binding protein with low pI</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Smac/DIABLO protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6R8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008003"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008101.1">
        <property type="protein sequence ID" value="ENSXCOP00000008003.1"/>
        <property type="gene ID" value="ENSXCOG00000006137.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007237"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
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        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to oxidative stress"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:neuron apoptotic process"/>
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        <property type="term" value="P:positive regulation of apoptotic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Smac/DIABLO-like_sf"/>
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        <property type="entry name" value="Smac_DIABLO"/>
    </dbReference>
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        <property type="entry name" value="DIABLO HOMOLOG, MITOCHONDRIAL"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR32247:SF3">
        <property type="entry name" value="DIABLO IAP-BINDING MITOCHONDRIAL PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF09057">
        <property type="entry name" value="Smac_DIABLO"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Smac/diablo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="215"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033049"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="239" mass="26823" checksum="40D4713B22A2F226" modified="2025-10-08" version="2">MFDLFSPRCLLVYLSVYDPIFVLFRISFRYLSRIKPAVRKSPRWTSVLASLAVGGSLCAVPFKQVENLSHDSLVRRAASLVTDSSSTFLSQATLALTDALTEYAKAVHTLIALQRQYLASLGKLSPAEEDTIWQVIISQRAEVAHRQDEQERFELTWVTAVRLCEAAAEAAYISGAENASITVRTNLQLAQSHVEEAQKVSRDAEKKLAETKVMEVERMTQHQGSNEEEETPEAYLRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8X2</accession>
    <name>A0A3B5L8X2_9TELE</name>
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        <submittedName>
            <fullName evidence="3">Ras association domain family member 9</fullName>
        </submittedName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="32473.ENSXCOP00000007262"/>
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        <property type="gene ID" value="ENSXCOG00000005616.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Ubiquitin-like_domsf"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="18"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="428" mass="48667" checksum="A934839432B00C5D" modified="2018-12-05" version="1">SALYGRTFVMMKHTDPVVGKEIQVTVCNEEKVVCGVTKHTTCTDMIQALLEDHKSLPESKRLLDGEPKDFCLVERWRGFERALPPLTRILRLWYAWGDEKPFIQFVLVKTSDFVPQTAKKGTKSKGARPKRWEFGRAQGAQPLPADKQKRMVRKAFRKLEQLHNENRNSPGAEEVERMVELILHQDHTIREQIQRMREVDSELECFQYRPRDDSKSLSDQACGLSLAANSEEQLEKYFYACDGIEQLETQVQQHKDFILQLSREIDGELRKFGLPPVPEEEGEQKGAAAATSLSETDDSFYKAEVERLQDELKRSLFSGMTLQNQVAEMDKQLIYVDGSLMAKDQECWQLAAQLNSLQTMDSMEETKLSLVLLKHEPEDSLTRAVKLKPSQSPADATDTDSDTGISSTHSQDSLSPCLDFPPPLDTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9W1</accession>
    <name>A0A3B5L9W1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="32473.ENSXCOP00000007270"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007363.1">
        <property type="protein sequence ID" value="ENSXCOP00000007270.1"/>
        <property type="gene ID" value="ENSXCOG00000005624.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000204465"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635:SF196">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017278638" description="Reverse transcriptase domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="269" mass="30850" checksum="901827593468CE66" modified="2018-12-05" version="1">MRNFSLGCPLSSLLYSLVAEPLAILIKQDETVKGIKSSQGNISKIFQFADDTTITVEDEASMDRVIVHLHNYGLASGAKINENKSEIMYCGVATRTPGRWEFREVVDEVKVLGVYLGKQQGEARDKTWENVISKVQKLLNLWNQRKLTIKGKTVIINSLIISKIIYPLSVYDLPDRILNKFNSQITTFIWRRNRNLINHKSLIAPYKEGGLKLVDILSKKQAIRIKLVTKFLTSSTKHIWFDYFRSYLESFGTKTVFNFEIKAAICNFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LA71</accession>
    <name>A0A3B5LA71_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LA71"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009088"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009199.1">
        <property type="protein sequence ID" value="ENSXCOP00000009088.1"/>
        <property type="gene ID" value="ENSXCOG00000006933.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000271625"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071300">
        <property type="term" value="P:cellular response to retinoic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045930">
        <property type="term" value="P:negative regulation of mitotic cell cycle"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045666">
        <property type="term" value="P:positive regulation of neuron differentiation"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033237">
        <property type="entry name" value="BRINP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15564:SF10">
        <property type="entry name" value="BMP_RETINOIC ACID-INDUCIBLE NEURAL-SPECIFIC PROTEIN 3 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15564">
        <property type="entry name" value="MACPF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017313274" evidence="1">
        <location>
            <begin position="21"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="83" mass="9336" checksum="11BBD66EF2BF3313" modified="2018-12-05" version="1">MGLAVLSLWLCCGWTSGVAGQGEGAPGSLEWLLSDKGPFHRSLEFQEALERYQQGFSTRYKIYRASKRLAPALLLAKLFWQML</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAX5</accession>
    <name>A0A3B5LAX5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TATA box-binding protein-like 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">TBP-like factor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAX5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006876"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006964.1">
        <property type="protein sequence ID" value="ENSXCOP00000006876.1"/>
        <property type="gene ID" value="ENSXCOG00000005313.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155712"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009792">
        <property type="term" value="P:embryo development ending in birth or egg hatching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd04517">
        <property type="entry name" value="TLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000005">
        <property type="entry name" value="TATA box-binding protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015445">
        <property type="entry name" value="TBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="182" mass="20224" checksum="31CD4F796D1CD759" modified="2018-12-05" version="1">MDSNNDEALDIIVTNVVATFRTRCHLNLRKIALEGTNVIYKPEVGKVLMKLRKPKITASIWSSGKIICTGATSEEEAKLGARRLARCLQKLGFKVRFSAFRVVNVLAVCSMPFAVHLVDFTKNNRPIASYEPELHPAATYRIKNLKATVQVFSTGSLTVTGMLLSHDHTHTHLPSLPSFIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBD4</accession>
    <name>A0A3B5LBD4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DBB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBD4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007820"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007918.1">
        <property type="protein sequence ID" value="ENSXCOP00000007820.1"/>
        <property type="gene ID" value="ENSXCOG00000006004.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008787"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036312">
        <property type="term" value="F:phosphatidylinositol 3-kinase regulatory subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052446">
        <property type="entry name" value="B-cell_PI3K-Signaling_Adptrs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017893">
        <property type="entry name" value="DBB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041340">
        <property type="entry name" value="PIK3AP1_TIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16267">
        <property type="entry name" value="BANK1/PIK3AP1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16267:SF12">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE ADAPTER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14545">
        <property type="entry name" value="DBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18567">
        <property type="entry name" value="TIR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01282">
        <property type="entry name" value="DBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51376">
        <property type="entry name" value="DBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DBB" evidence="3">
        <location>
            <begin position="163"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="481"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="647"/>
            <end position="670"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="735" mass="82915" checksum="7AAD34EDE03F03C2" modified="2018-12-05" version="1">MLCFAAISEFSSAFELLILHTEEAQEWAAYLQQILKSSKHFRRGSVLLYALSSADQLHGYNFEDFQSCKCIVVLLTGFFLDVLYDPDLHGALQRLLYPPHRVVALLCGVTEADLNVEYFEGWPSWRKLYADDEPGVYVSTVLESIEESEEQSETFLKEEERAIFTLVCSVQDQETIFIVFRSRVGFGSPVEVEFSPEGAASKRVLASVENEFTISVSAPGVTSLALHADGSRVGLSPVTFYTSMGEVSRHLKQATDPVNFICQAFNLTSNATESVDTLLTDSLKSRMPPSGLQLFGVKQIEEDNMSAYQRKEELPTLLHFAAKYGLKKLMSSLLRCPGALQAYSVMNKHGDYPNTLAEKSGFQDLRRFMDEFVETADLLQSHLEDNLNLDNDAEIYEAEIYEPMSNSSQDIMMKYSGCTEDIYESMLAKPHAAENQVNAPPPAAENNDRNEHVNFDEIEEEEDPYNICPEDIYDIVDTNSTYNPGVQNRPPAPIPRPESEPEPERPATYISRGLLFTVKFLHTGPAQARPVSEAPCSTYDPYGGMKTPGQRQLICLQERVKVGEITVDEAVQEFKAWQFDHERRANSLRYQQDNLKKLRDSITRRHKEREKTGQEIDYEISAPLQRSLYWGSNSTLECSVYEPTPRMVAPPPPATQPIKRGSWKTGSTSSTSSESLVILFLGMNIIEYGVIRSEKSCPCDEQAVCSWCRHMGGRNGYQDTHAKKLFLFRKDMETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBP9</accession>
    <name>A0A3B5LBP9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBP9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009727.1">
        <property type="protein sequence ID" value="ENSXCOP00000009613.1"/>
        <property type="gene ID" value="ENSXCOG00000007327.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000271297"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="327" mass="33672" checksum="284E9D9D7D112944" modified="2018-12-05" version="1">MQQDSQLGPSPGYTHGSSPGSSPGPRDGPSPGPGDGPSSGSRPGPSPGPRHGPSPGSRYGPSPGSSPGPRDGPRDGPSPGAQRWTQPWIQCWFQTWTNPRFQAWSQTWSQPWTQPWFPTWTQPYMDPAQVPSLVPDMVPAMDPALVPALVPEMDQEMDPDLVPGNGPSTGSSAGSRHRPSPGSKPGPRHGPGHGPSPGSNGPSPGSSPGLRHGPSPGPRYGPGTLSALVPAILPALVPALFQPLSRPFFQPWSWSSSTPVSQISPGPGSRHGFSPCSSPGFSPGPGDGPNPVPSHGPRHGPSPGSRYGPSTGPRDGLRDGPSPGARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LC45</accession>
    <name>A0A3B5LC45_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Clarin 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CLRN3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the clarin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027884933.1">
        <property type="nucleotide sequence ID" value="XM_028029132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LC45"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007281"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007375.1">
        <property type="protein sequence ID" value="ENSXCOP00000007281.1"/>
        <property type="gene ID" value="ENSXCOG00000005637.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114151750"/>
    <dbReference type="GeneTree" id="ENSGT00850000132319"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026748">
        <property type="entry name" value="Clarin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31548">
        <property type="entry name" value="CLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31548:SF3">
        <property type="entry name" value="CLARIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25807">
        <property type="entry name" value="Clarin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="229" mass="25246" checksum="82ECA29626FEB1B0" modified="2018-12-05" version="1">MPSSTKILYFLLSAMLTAISIGLLGYAMSTTWSKTLMDCEGFGTNNGSAEITMGLFSGNLVRNTCPSFGSEETFPVFPALSAIEITPLVLHGLCVGLLAVCFLTSALSILISFYNSVSNPYETYMGPNGIYTCSSISACSSVLVIIIYVLNLLLTKMQEELVPSIADISNVELTHKRLEVRLGYFLLIPYAVLSLLAILFIYLYDHAAYTHRREQERPTEDAPKEIMMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LD48</accession>
    <name>A0A3B5LD48_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alkyl transferase</fullName>
            <ecNumber evidence="4">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>n isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate = a di-trans,poly-cis-polyprenyl diphosphate + n diphosphate</text>
            <dbReference type="Rhea" id="RHEA:53008"/>
            <dbReference type="Rhea" id="RHEA-COMP:19494"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:136960"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.87"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LD48"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010103"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010222.1">
        <property type="protein sequence ID" value="ENSXCOP00000010103.1"/>
        <property type="gene ID" value="ENSXCOG00000007664.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007879"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF43">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT DHDDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="317" mass="36847" checksum="C77AA2A80B790927" modified="2025-10-08" version="2">MQWSGPMPKHVAFIMDGNRRFARKKKLERHDGHMQGFNKLAETLRWCKHLNIPEVTVYAFSIENFKRSKEEVDGLLDLARQKFERLLEEQENLEKHGVCIRVLGDLNMLPLDLQQVIAKAVVMTKNHNKCFLNVCFAYTSRYEITNAVREMAWGVEQGLIKASDVSEALLSECLYSNNSPNPDLLIRTSGEVRLSDFLLWQTSHSCLVFQSVLWPEYSFWNLCEAIVQYQLNHKSIQKARELHREEQALQQLEADCACIAEHLQHQGNGKPADAQRRQEALQHYTVTREERVQDFLQALKHKRDTFFTNLCSETVLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDE9</accession>
    <name>A0A3B5LDE9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Amine oxidase</fullName>
            <ecNumber evidence="11">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the flavin monoamine oxidase family. FIG1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDE9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010198"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010318.1">
        <property type="protein sequence ID" value="ENSXCOP00000010198.1"/>
        <property type="gene ID" value="ENSXCOG00000007733.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160928"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001716">
        <property type="term" value="F:L-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1620:FF:000001">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.405.10:FF:000004">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000242">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000450">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001613">
        <property type="entry name" value="Flavin_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF342">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00757">
        <property type="entry name" value="AMINEOXDASEF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017321112" description="Amine oxidase" evidence="12">
        <location>
            <begin position="20"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Amine oxidase" evidence="13">
        <location>
            <begin position="62"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="82"/>
            <end position="83"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="106"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="477"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601613-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="513" mass="58001" checksum="23D486B2B66DC23D" modified="2018-12-05" version="1">NEPHLIPLLLLSECRTVAAISVKEDLAKCLNDTDYNDLMNTMTHGLPAIQTPHHVVIVGAGMAGLTAAKLLEKAGHQVTIVEASGRVGGRVQTHRNENEGWYADLGAMRIPSYHYIVRSLIQEFGLKMNKFRMVDMNTFYYVHGLRSKTYSVMQDPDVLKYNLPKRERHIAPLKLLEMALRKVKEEVKAHGCKAMLKKYDHYSVKEYLLTEGGLSAEAVRMIGDLLNENSLMHLALTEMIYIENDVSDATTYDEITGGTDLLAKAFLTVLDAPILLDSRVRRIQRSHKGVTVWYQRENDPSLESLQADAVLVTTTAKAALFIEFDPPLSTRKMEAMRAVHYESSTKIILTFKEKFWTKDGIIGGKSITDRPIRYTYYPSHSFPGNDRIGVLLASYTWGDDSHLFQGASDEELKELALRDLMHIHGKNIRNLCTGVIVKKWELDPYSLGAFALFTPYQHIEYSAELFRHEGHIHFAGEHMAFPHAWIETSMKSAIRAAQNINNAVHKDSCKTEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDJ8</accession>
    <name>A0A3B5LDJ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDJ8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008751.1">
        <property type="protein sequence ID" value="ENSXCOP00000008645.1"/>
        <property type="gene ID" value="ENSXCOG00000006610.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007479"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042415">
        <property type="term" value="P:norepinephrine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042428">
        <property type="term" value="P:serotonin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16554">
        <property type="entry name" value="RING-HC_RNF180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033263">
        <property type="entry name" value="RNF180"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045790">
        <property type="entry name" value="RNF180_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46717">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46717:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19332">
        <property type="entry name" value="RNF180_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="266"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="416" mass="47482" checksum="CDD064A3836A8521" modified="2018-12-05" version="1">MEYIYFDTFIKPPHPSVKFIVVLPVCVFLRSSLCLQAADCGPVSCCIWHMDVDNLPEWIWISVQQAVWTSGKLNCFHCGARLGGYSFLNRYRCPCGRDATVHFSRSRVDLDQKPRLLIVPRRRMGRGSAQAQQEGPALDLVCAGGTPQHGAARDTDVKKLNGSEPKPGETLVELLVLTRFWLSPGQVSSSQGPVVLSSLARTRLSKTDQNRLKSRRRKQRRRERWLQSQLQEEEEEEPVRKNYKLLLLLLKVDLFQSDVDRDGLTCAVCLDVFFRPRSCLPCAHVFCEPCLRRLARNRAAHTPCPLCRRLISNTGFHRELDHRAKTLFPKVWATRRQNFQNSLSARWPLPSSREQTRYLCCQDIFTFILLKGNQDEVPHRVFRGLLVVSFSGSWCSAPTGQEGNRLVCLTITTKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDN2</accession>
    <name>A0A3B5LDN2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tumor necrosis factor, alpha-induced protein 8-like 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TNFAIP8 family. TNFAIP8L2 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027868177.1">
        <property type="nucleotide sequence ID" value="XM_028012376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDN2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007796"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007894.1">
        <property type="protein sequence ID" value="ENSXCOP00000007796.1"/>
        <property type="gene ID" value="ENSXCOG00000005988.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114141682"/>
    <dbReference type="KEGG" id="xco:114141682"/>
    <dbReference type="CTD" id="393345"/>
    <dbReference type="GeneTree" id="ENSGT00390000003488"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.160:FF:000001">
        <property type="entry name" value="Tumor necrosis factor alpha-induced protein 8-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.160">
        <property type="entry name" value="Tumor necrosis factor alpha-induced protein 8-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008477">
        <property type="entry name" value="TNFAIP8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038355">
        <property type="entry name" value="TNFAIP8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12757:SF4">
        <property type="entry name" value="TUMOR NECROSIS FACTOR ALPHA-INDUCED PROTEIN 8-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12757">
        <property type="entry name" value="TUMOR NECROSIS FACTOR INDUCED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05527">
        <property type="entry name" value="TNFAIP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="186" mass="21184" checksum="1F6E681A4F00E43E" modified="2018-12-05" version="1">MEAFNSKDLAMKAQKKIFSSMANKSTVQRFIDDTTSEILDELYRVSKEYTGNKGVAQKVIKNLIKIAVKIGVLFRNNCFSEEELKLAQEFKKKLHTGAMTAISFYEVDFTFDKTVMSDNLTECKDLLLKLVNSHLTSKSHDRISHVFNHYSDPQLLTKLYDPSGPFRPHLTKICLGLNKLVEDGTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LE86</accession>
    <name>A0A3B5LE86_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Nucleoside diphosphate kinase homolog 7</fullName>
            <shortName evidence="13">NDK 7</shortName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Major role in the synthesis of nucleoside triphosphates other than ATP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 13 15 16">Belongs to the NDK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LE86"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007996"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008094.1">
        <property type="protein sequence ID" value="ENSXCOP00000007996.1"/>
        <property type="gene ID" value="ENSXCOG00000006138.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158946"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005879">
        <property type="term" value="C:axonemal microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04412">
        <property type="entry name" value="NDPk7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000004">
        <property type="entry name" value="Nucleoside diphosphate kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006602">
        <property type="entry name" value="DM10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011410">
        <property type="entry name" value="NDPK7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037993">
        <property type="entry name" value="NDPk7B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43109">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43109:SF2">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036503">
        <property type="entry name" value="NDK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00676">
        <property type="entry name" value="DM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51336">
        <property type="entry name" value="DM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DM10" evidence="17">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="14">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR036503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR036503-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="341" mass="38174" checksum="2FA61E58B6F92ECC" modified="2018-12-05" version="1">ALLRRYQLFFYPKDNSVEMFDLKYQRMFLRRTKYDDLHLEDLFVGNRVTVFSRQLKLIDYGDQFTANKLGSRIEKTFALVKPDVLNKIGDIFGLIYSANLIVTKAKMTKLTCTLIQFIISGPVVAMELTGDEAICIWRTLLGPPDSAAARQEAPQSIRAKFGTDAVRNVCHGSESLAAAARELEFFFPSTVGRGPVNSAVCEDSTCCIIKPHAITEGLTGKILNTIHEARFKISALQMFNMDLANAEEFYEIYKGVVTEYPGMVSELCSGSCIVLEIQGADTPKSFRELCGPSDPEIARHLRPNTLRGLYGKDKVRNAVHCTDLPEDGVLEVQYFFRILDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LED5</accession>
    <name>A0A3B5LED5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Transforming growth factor beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Required to maintain the Transforming growth factor beta-1 (TGF-beta-1) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-1 and regulates its activation via interaction with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS, that control activation of TGF-beta-1. Interaction with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-1.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Required to maintain the Transforming growth factor beta-3 (TGF-beta-3) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-3 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-3. Interaction with integrins results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-3.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Transforming growth factor beta-3 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains, which constitute the regulatory and active subunit of TGF-beta-3, respectively.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Transforming growth factor beta-3: Multifunctional protein that regulates embryogenesis and cell differentiation and is required in various processes such as secondary palate development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-3 (TGF-beta-3) chains remain non-covalently linked rendering TGF-beta-3 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP that control activation of TGF-beta-3 and maintain it in a latent state during storage in extracellular milieus. TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3. Once activated following release of LAP, TGF-beta-3 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Latency-associated peptide: Homodimer; disulfide-linked. Latency-associated peptide: Interacts with Transforming growth factor beta-1 (TGF-beta-1) chain; interaction is non-covalent and maintains (TGF-beta-1) in a latent state; each Latency-associated peptide (LAP) monomer interacts with TGF-beta-1 in the other monomer. Transforming growth factor beta-1: Homodimer; disulfide-linked. Transforming growth factor beta-1: Interacts with TGF-beta receptors (tgfbr1 and tgfbr2), leading to signal transduction. Interacts with EFEMP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16 18">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LED5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006224"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006298.1">
        <property type="protein sequence ID" value="ENSXCOP00000006224.1"/>
        <property type="gene ID" value="ENSXCOG00000004816.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155747"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005160">
        <property type="term" value="F:transforming growth factor beta receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000004">
        <property type="entry name" value="Transforming growth factor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000006">
        <property type="entry name" value="Transforming growth factor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016319">
        <property type="entry name" value="TGF-beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015618">
        <property type="entry name" value="TGFB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF34">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR BETA-3 PROPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001787">
        <property type="entry name" value="TGF-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01423">
        <property type="entry name" value="TGFBETA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01426">
        <property type="entry name" value="TGFBETA3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 16 18" id="KW-0339">Growth factor</keyword>
    <keyword evidence="11 16" id="KW-0497">Mitogen</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 16" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017107046" description="Transforming growth factor beta" evidence="16">
        <location>
            <begin position="20"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="19">
        <location>
            <begin position="292"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="310"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="339"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="343"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain" evidence="17">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR001787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR001787-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="407" mass="46716" checksum="6E91C6C693113BCC" modified="2018-12-05" version="1">MHLGKALLFVLLLNCATLSSPLSTCATVDIDHVKRKRVEAIRGQILSKLRLSSPPHSLGPSKVPYQIQALYNSTKELLEQLGRDRKQSCGQDNTETEYIAKEIYKINMVYGTPDNNNLPCPKSLTSKVFRFNVSEMERNSTNLFRAEFRALRVPNPKAMRSEQRIELYQIMRLNEHIRKQRFIAAKNVQTRGSPEWVTFDVTETVREWLINKGSNLGLEISVHCPCHTFNSNGDIIDNEKEVLEVKFRGVDGDEEQSRLDLDNLKKSKQQHPPHLILMMIPPHRMDTHSSRRRKRALDTNYCFSNTEESCCVRRLHIDFRRDLDWKWIHEPSGYDANYCSGPCPYLRSSDTTHSSLLSLYNTLNPEASAAPCCVPQDLEPLTILYYSGRTPKVEQLSNMIVKSCKCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFY4</accession>
    <name>A0A3B5LFY4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFY4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009767"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009881.1">
        <property type="protein sequence ID" value="ENSXCOP00000009767.1"/>
        <property type="gene ID" value="ENSXCOG00000007420.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286981"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003288">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002274">
        <property type="entry name" value="Zinc finger protein 432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="60" mass="6859" checksum="0CEF26E4F41B82EE" modified="2018-12-05" version="1">YGQTPFSCPSCRKQFIQKGHLTGHMRIHTGEKPFLCEICGKSFSQKSYLSQHHRAHDGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGD5</accession>
    <name>A0A3B5LGD5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Splicing factor 3B subunit 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pre-mRNA branch site protein p14</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">SF3b 14 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGD5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011203"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011333.1">
        <property type="protein sequence ID" value="ENSXCOP00000011203.1"/>
        <property type="gene ID" value="ENSXCOG00000008477.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005908"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12241">
        <property type="entry name" value="RRM_SF3B14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000106">
        <property type="entry name" value="Splicing factor 3b, subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034150">
        <property type="entry name" value="SF3B6_RRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="19"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00070534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00082208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="129" mass="14713" checksum="778785BB747C76F8" modified="2018-12-05" version="1">MAMQAAKRANIRLPPEVNRILYVRNLPYKITAEEMYDIFGKYGPIRQIRTGNTPETRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYNANRVRLSGTAFHSLVPKQSQIITLCRKGLIRHVGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGH8</accession>
    <name>A0A3B5LGH8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
            <location evidence="1 11">Extracellular space</location>
            <location evidence="1 11">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGH8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009705"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009819.1">
        <property type="protein sequence ID" value="ENSXCOP00000009705.1"/>
        <property type="gene ID" value="ENSXCOG00000007382.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159166"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000001">
        <property type="entry name" value="Wnt homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009140">
        <property type="entry name" value="Wnt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF93">
        <property type="entry name" value="PROTEIN WNT-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01842">
        <property type="entry name" value="WNT2PROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0879">Wnt signaling pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="391" mass="43653" checksum="F7652F8920580745" modified="2018-12-05" version="1">MLGLRRILGLRAARIHRGGSPGAGLSPGPSSCQNQGASSRMYCACLLLLLLVTPRVDSSWWYIGALGARVICDNIPGLVNKQRQLCQRHPDIMQAIGEGTKEWIRECQHQFRHHRWNCSTLDRDHTVFGRVLLRSSREAAFVYAISSAGVVYALTRACSQGELKLCNCDPLRRGRANDGKGDFDWGGCSDNVNYGIKFAKTFIDAKERPIRDGRALMNLHNNRCGRTAVKRFMNMECKCHGVSGSCTLRTCWMALSDFRKTGDYLRRKYNGAVEVIMNQDGTGFTVANKAFRKATKNDLVYFENSPDYCLQDKAAGSLGTAGRVCNKTSRGTDGCEVMCCGRGYDTTRVMQITKCECKFKWCCAVECKDCEEAVDIHTCKAPKRAEWLDKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGQ8</accession>
    <name>A0A3B5LGQ8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGQ8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010161.1">
        <property type="protein sequence ID" value="ENSXCOP00000010042.1"/>
        <property type="gene ID" value="ENSXCOG00000007620.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063187"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031462">
        <property type="term" value="C:Cul2-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051341">
        <property type="entry name" value="Zyg-11_UBL_adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12904">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12904:SF22">
        <property type="entry name" value="ZYG-11 FAMILY MEMBER B, CELL CYCLE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="342" mass="37479" checksum="02709275C02A76AE" modified="2018-12-05" version="1">MHHLITFRCVLQDGEGPQTLCDLCLAQVCSRLDALCRKGADGSMSLIWAPLFPQELADQLLQMMATKGMLNDVTVGIFRNSEKLRLRSASIRYCKVSAKAFRLALCPHRLLELDASWVPGGLTGADVILGLASNPDCRASLQMLALNGLSLNLGSPEKDGGVYVGFSSLQGLKTLHLVNTDLTDDILEDICSLPRLETLDISCSSVSELNALLKCKNTLRSLIAHRLYRLDTSPVRLLFIFAQLHALRHLDFSNNHFANDDSESRVDGDETVRQLLEAGPQILPSLLSLDISGRKTINDSALRGFVESRSGLVFLGLLATGLSSSPVLSSHENLKVSRCFWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LI10</accession>
    <name>A0A3B5LI10_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ATPase family AAA domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000010245"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010368.1">
        <property type="protein sequence ID" value="ENSXCOP00000010245.1"/>
        <property type="gene ID" value="ENSXCOG00000007777.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074694"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05528">
        <property type="entry name" value="Bromo_AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19517">
        <property type="entry name" value="RecA-like_Yta7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000021">
        <property type="entry name" value="ATPase family AAA domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000016">
        <property type="entry name" value="ATPase family AAA domain-containing protein 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000699">
        <property type="entry name" value="ATPase family AAA domain-containing protein 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000061">
        <property type="entry name" value="ATPase family, AAA domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045199">
        <property type="entry name" value="ATAD2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069:SF5">
        <property type="entry name" value="ATPASE FAMILY AAA DOMAIN-CONTAINING PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 17" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Bromo" evidence="19">
        <location>
            <begin position="923"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="160"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1031"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1099"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1206"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="181"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="261"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="291"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1165"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1206"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1226"/>
            <end position="1237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1333" mass="149837" checksum="F52028C19234BAD8" modified="2018-12-05" version="1">DNFTNKEPSSDASSPNSPRLAPPAKRTRRQTASEPSSSDTSPSPQAKGQRVSWGLPTYRTRLSSRIMQNGHGNSESGGADHSRMNGHVELKRSCRVRKSQFQHLNQSLLFDQLVNSTAEAVLQEMDNITSIRQNRELERLRMWNGETEEENMDMYSRVKRRKSMRRSTFNMPAHHKVMSKDEEEEEEEEGTEESNGEEEEEEDAEDDDEDDEGDEAEEAGEENDRPYNLRQRKTVQRYEAPPIEPVNRKQSNPSLFDTHRSPARRSHIRVKKHAIHSSDTTSSSDEERFERRKSKSMTRARNRCLPINLTPDSLSSGVLRDRVKVGTSLADVDPMNLDNSVRFDNVGGLSNHIQSLKEMVVFPLLYPEVFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDRKVAFFMRKGADCLSKWVGESERQLRLLFDQAYLMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDSRGEIVVVGATNRLDSIDPALRRPGRFDREFLFSLPDKKARKQILEIHTRDWDPKLSEPFLNELAEKCVGYCGADIKALCTEAALMALRRRYPQIYGSSVKLKLDVASIVLGPGDFSKAMRTIVPASQRALAPAGRALSPTLRPLLGCSFSSVLKTLLRVFPHAQCTDRDNLHAGDNQLLEEEFYSDDDNEDGSASIYEVPPSACPKSHLSSSAMHRPFLHFSSSSLQQPTAYRPRMLLAGAPGSGQSSHLAPALLHHLDKLPVHRLDLPTLYSVSAKTPEESCAQVFREARRSVPSVVYMPHISEWWDTVSDTVKSTFLTLLNDVPSFSPVLILATAETQYSRLTEEVKSIFQRTYGEVVLLCPPGDQERRSFFSDLLLVQAARSPPRPRNSERCEEVLAVAETPAPRVLSAEEQRRLAEQEDNTLRELRLFLRDVTKRLATDKRFSIFSKPVDIEEVSDYLEVIRQPMDLSTVMTKIDTHQYLTAKDFLADIDLISSNALEYNPDKDPGDKVIRHRACSLKDTAHAIFAAELDPEFDRMCEEIKEARKKRDFQTVGQPTSAPGALPTRKQHPAGDEQAKTNTQGEAAFKHCTANHLKRKICRRPSWKRGIIRKKKNYKKGTEEEEAEAEAEATVPSDSCLTQDEESSCDTPGPQANGHHTHGPSTEEESSNEPPIATAAKEGQAETPEKRTEQRCLCGTPPPNSEEVDCVNGTESMDSVDLHSLKRSNEADTRTPPSSADGCNKPEQEEHKDINVSKQTQEQHPLDGDAVTQTVEEDQGKEGACVLLSLVVKKSEGYSVEQLERLYSLLSQCIYRHRREYDKTRLLQVRTPLPPGIPSHWSRVQILVPTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LI78</accession>
    <name>A0A3B5LI78_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Calx-beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Ca(2+)(in) + 3 Na(+)(out) = Ca(2+)(out) + 3 Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:69955"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC8 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LI78"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007564"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007660.1">
        <property type="protein sequence ID" value="ENSXCOP00000007564.1"/>
        <property type="gene ID" value="ENSXCOG00000005831.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164137"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005432">
        <property type="term" value="F:calcium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000003">
        <property type="entry name" value="sodium/calcium exchanger 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051171">
        <property type="entry name" value="CaCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038081">
        <property type="entry name" value="CalX-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003644">
        <property type="entry name" value="Calx_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004836">
        <property type="entry name" value="Na_Ca_Ex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032452">
        <property type="entry name" value="Na_Ca_Ex_C-exten"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00845">
        <property type="entry name" value="caca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878:SF73">
        <property type="entry name" value="NCX4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11878">
        <property type="entry name" value="SODIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03160">
        <property type="entry name" value="Calx-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16494">
        <property type="entry name" value="Na_Ca_ex_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01259">
        <property type="entry name" value="NACAEXCHNGR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00237">
        <property type="entry name" value="Calx_beta"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141072">
        <property type="entry name" value="CalX-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="12" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="547"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="624"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="654"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="695"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="21">
        <location>
            <begin position="203"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Calx-beta" evidence="21">
        <location>
            <begin position="320"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="723" mass="80583" checksum="683F332400FF8C47" modified="2018-12-05" version="1">MFIIIALCVYVVPESETRKIKHLRVFFVTAAWSVFAYIWLYLILSVFSPGEVEVWEAVLTFLFFPLCVLQAWIADRRLLFYKYVNKRYRANKNRGIIIESEGDEALDGMLEGVEEGGGMSAEQDQEEEARREMARTLKDLKQRHPDKDMEQLIEMANYQVLVQQQKSRAFYRIQATRMMIGAGNILKKHAADQARKVVSCHEASGQEEDPNTIYLQFDPSHYQCFENCGSLKLTVSRHGGEAGCTVKVDYRTEDATATAGSDYEFAEGTLVFKPGETTKDFTVGVIDDDIFEEDEHFYVRLSNPRIVHRAEAALGNAPVATVTIYDDDHAGIFTFESNSTRVSESVGNMQVKVHRTSGARGKVAVPYHTVEGTAKEGEDYDEVSGKLEFQNDETMKLLNVKIIDDEEYEKNKTFTIVLEEPVLLEFLLLLICAGDTNDNKMAVGPEDVSKMGCPVLGEHTKLEVVIEESYEFKSTVDKLIKKTNLALVVGSSSWREQFVSAVTVSAGDDDEEESGEERLPSCFDYIMHFLTVFWKVLFAFVPPTEYWNGWACFFVSISLIGVLTAVTGDLASHFGCTVGLKDSVTAVVFVALGTSVPDTFASKVAAIQDQYADASIGNVTGSNAVNVFLGIGVAWTIAAVYWKTKGQTFKVNPGSLAFSVTLYTVMALVCVLVLLYRRRPSVSGGELGGPRTAKLLTFFLFLSLWFIYILLASLEAYCHVPGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LII3</accession>
    <name>A0A3B5LII3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRM elav family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LII3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010682"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010806.1">
        <property type="protein sequence ID" value="ENSXCOP00000010682.1"/>
        <property type="gene ID" value="ENSXCOG00000008068.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155528"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000480">
        <property type="entry name" value="Fne, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002343">
        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="10"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="96"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="144" mass="15962" checksum="808A435659015C68" modified="2018-12-05" version="1">MMEEDDDSRTNLIVNYLPQSMSQDELRSLFSSVGEVESAKLIRDKVAGHSLGYGFVNFVNHSDAERAISTLNGLRLQSKTIKVSFARPSSDTIKDANLYISGLPRTLSQQDLEDMFSSFGRIINSRVLVDQQRSAEPGSSDSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIY3</accession>
    <name>A0A3B5LIY3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription initiation factor IIB</fullName>
            <ecNumber evidence="4">2.3.1.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">General transcription factor TFIIB</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions. Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element. Modulates transcription start site selection. Also exhibits autoacetyltransferase activity that contributes to the activated transcription.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIY3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009611"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009725.1">
        <property type="protein sequence ID" value="ENSXCOP00000009611.1"/>
        <property type="gene ID" value="ENSXCOG00000007322.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006671"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061733">
        <property type="term" value="F:protein-lysine-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000979">
        <property type="term" value="F:RNA polymerase II core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990114">
        <property type="term" value="P:RNA polymerase II core complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001174">
        <property type="term" value="P:transcriptional start site selection at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20551">
        <property type="entry name" value="CYCLIN_TFIIB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20552">
        <property type="entry name" value="CYCLIN_TFIIB_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000008">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000019">
        <property type="entry name" value="transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000003">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF77">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Cyclin-like" evidence="20">
        <location>
            <begin position="84"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="20">
        <location>
            <begin position="178"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00056112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="281" mass="31151" checksum="054B403AAD67281C" modified="2018-12-05" version="1">CIFLFHSDRVIDVGSEWRTFSNEKALKDPSRVGDAQNPLLNGGDLTTMISRGTGAASFDEFGNSKYQNRRTMSSSDRAMLNAFKEISTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLGLPKQVQMAATFIARKAVELDLVPGRSPISVAAAAIYMASQASAEKKTQKEIGDIAGVADVTIRQSYRLIYPRAAELFPPDFKFDTPVDKLPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKW6</accession>
    <name>A0A3B5LKW6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Retinoic acid receptor gamma a</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">Heterodimer; with an rxr molecule. Binds DNA preferentially as a rar/rxr heterodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027891415.1">
        <property type="nucleotide sequence ID" value="XM_028035614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKW6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011315"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011446.1">
        <property type="protein sequence ID" value="ENSXCOP00000011315.1"/>
        <property type="gene ID" value="ENSXCOG00000008543.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114155510"/>
    <dbReference type="CTD" id="30606"/>
    <dbReference type="GeneTree" id="ENSGT00940000156458"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035259">
        <property type="term" value="F:nuclear glucocorticoid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071376">
        <property type="term" value="P:cellular response to corticotropin-releasing hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001889">
        <property type="term" value="P:liver development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060037">
        <property type="term" value="P:pharyngeal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048384">
        <property type="term" value="P:retinoic acid receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06964">
        <property type="entry name" value="NR_DBD_RAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06937">
        <property type="entry name" value="NR_LBD_RAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000001">
        <property type="entry name" value="Retinoic acid receptor beta isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000004">
        <property type="entry name" value="Retinoic acid receptor beta isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047159">
        <property type="entry name" value="NR_DBD_RAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047158">
        <property type="entry name" value="NR_LBD_RAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003078">
        <property type="entry name" value="Retinoic_acid_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24085">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24085:SF7">
        <property type="entry name" value="RETINOIC ACID RECEPTOR GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01292">
        <property type="entry name" value="RETNOICACIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="15">
        <location>
            <begin position="74"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="16">
        <location>
            <begin position="173"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="410"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="420"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="457"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="503" mass="56469" checksum="6FE9AF6DEFFE9D41" modified="2018-12-05" version="1">MFDCMEALGMGPRQLYDVTSRGACMLRKASPFFPALDPFAWTGSASVRSVETQSTSSEEMVPSSPSPPPPPRVYKPCFVCQDKSSGYHYGVSSCEGCKGFFRRSIQKNMVYTCHRDKNCQINKVTRNRCQYCRLQKCFEVGMSKEAVRNDRNKKKKDVKEEVVLPESYELSGELEELVNKVSKAHQETFPSLCQLGKYTTNSSSDHRVQLDLGLWDKFSELSTKCIIKIVEFAKRLPGFTTLTIADQITLLKSACLDILMLRICTRYTPEQDTMTFSDGLTLNRTQMHNAGFGPLTDLVFAFAGQLLPLEMDDTETGLLSAICLICGDRMDLEEPEKVDKLQEPLLEALKIYARRRRPNKPHMFPRMLMKITDLRGISTKGAERAITLKMEIPGPMPPLIREMLENPDAFDDQTECNENAPPPPPPPLPPPPPALVVKQEAEDEDDSWGTENCSEPSPEEEDEDDDDDLGDEERDRGSDSDGEPWGVLDNLDGSRKGLVGRAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMX1</accession>
    <name>A0A3B5LMX1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">May be involved in the intracellular transport of sterols or other lipids. May bind cholesterol or other sterols.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMX1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012322"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012471.1">
        <property type="protein sequence ID" value="ENSXCOP00000012322.1"/>
        <property type="gene ID" value="ENSXCOG00000009316.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159159"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120020">
        <property type="term" value="F:cholesterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070508">
        <property type="term" value="P:cholesterol import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043556">
        <property type="entry name" value="StARD5/6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002913">
        <property type="entry name" value="START_lipid-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46374">
        <property type="entry name" value="PROTEIN CBG07384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46374:SF3">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01852">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00234">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50848">
        <property type="entry name" value="START"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="3" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="START" evidence="5">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="193" mass="21572" checksum="A8926AE163DF73C4" modified="2018-12-05" version="1">MDYEHKAKAVADCLLSYRRDESGWKVCRKSNDVVVSWRPSSEFPGNVYKGEGIVSGTPENVWECLKPVPNGLRVKWDSNVKRFELLEQIKEDISICRTVTPSAAMGIIAPRDFVDVILVKQYEDGSISSNGAFPSYPPALHTHMEPSKTQVSSFFQTDLSGLLPRSVVDSFFPSSMTEFYSNLAKAVKSLKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNX5</accession>
    <name>A0A3B5LNX5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Nuclear factor, interleukin 3 regulated, member 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family. NFIL3 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027882775.1">
        <property type="nucleotide sequence ID" value="XM_028026974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNX5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012722"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012876.1">
        <property type="protein sequence ID" value="ENSXCOP00000012722.1"/>
        <property type="gene ID" value="ENSXCOG00000009614.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114150533"/>
    <dbReference type="KEGG" id="xco:114150533"/>
    <dbReference type="CTD" id="100000898"/>
    <dbReference type="GeneTree" id="ENSGT00940000164108"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14694">
        <property type="entry name" value="bZIP_NFIL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000025">
        <property type="entry name" value="nuclear factor interleukin-3-regulated protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047229">
        <property type="entry name" value="NFIL3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047106">
        <property type="entry name" value="NFIL3-like_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15284:SF0">
        <property type="entry name" value="GH23983P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15284">
        <property type="entry name" value="NUCLEAR FACTOR INTERLEUKIN-3-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="41"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="105"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="198"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="105"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="245" mass="27788" checksum="D4125C9317FBE1C5" modified="2018-12-05" version="1">MESLSFPSQRGEDSNVFHVESDLVRKGPRRKREFIPEEKKDALYWEKRRKNNEAAKRSREKRKLNDYALESHFMALKEENTRLSAELMAIKLHFGLSQSTAYTGHQSNQLQHHSHSSIQPSTYNQSLQGNPYWSARDSSSVANHQSSYPFLIPAYALHTMRGYSYLNGSGTSGSGVLAPLVLPRNLLPPYSACPGAPLPKPYPTRANSDEEEEQQVPGVLYRSVPRHKTSTRKDKGSPLRQYISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPR8</accession>
    <name>A0A3B5LPR8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000014103"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014279.1">
        <property type="protein sequence ID" value="ENSXCOP00000014103.1"/>
        <property type="gene ID" value="ENSXCOG00000010684.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000271184"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="213" mass="23951" checksum="6164F081C26002B1" modified="2018-12-05" version="1">MVSQLLLPNQPALQPAHPRPNQPAHQPAHPRPRPSVFLPRPRPSVFLPRPRPSVFLPRPRPSVFLPRPRPSVFLPRPRPSVFLPRPRPSVFLPRPRPSVFLPRPRPSVFLPRPRPSSDLVTPPLADKVGYVAGPAKNLLPGFSFPGPIDSGRARIQRVFLCLTAGEVRDPGFRHXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNMWNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPW8</accession>
    <name>A0A3B5LPW8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPW8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013047"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013207.1">
        <property type="protein sequence ID" value="ENSXCOP00000013047.1"/>
        <property type="gene ID" value="ENSXCOG00000009888.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156617"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13948">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13948:SF21">
        <property type="entry name" value="RNA-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="G-patch" evidence="4">
        <location>
            <begin position="38"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="107" mass="11819" checksum="1B547D0F50B6425C" modified="2018-12-05" version="1">NFLQEASQLLTILQGLQPPLKLDGKTIGVDYAKNGLTSDNIGNKMLQAMGWKEGKGLGRNQQGITAPIEVHTVKLEIMGSISLSSINYYTYKDAVRKAMFARFTELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LT29</accession>
    <name>A0A3B5LT29_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LT29"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015163"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015353.1">
        <property type="protein sequence ID" value="ENSXCOP00000015163.1"/>
        <property type="gene ID" value="ENSXCOG00000011491.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063891"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039878">
        <property type="entry name" value="RBM33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22014">
        <property type="entry name" value="RNA-BINDING PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22014:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="99" mass="11134" checksum="AE5617C03BA45A8E" modified="2018-12-05" version="1">GRGRGRGTGPMGRGHPLPTRQSQRGAEKEHSTVFSCVLDEYLSLFSSFLLCLLQMFKMMPQQRKAIAKFSNPQHAASFQMSFHRHMIDLSHIDVSLTDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LT63</accession>
    <name>A0A3B5LT63_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transmembrane protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TMEM11</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in mitochondrial morphogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMEM11 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027898721.1">
        <property type="nucleotide sequence ID" value="XM_028042920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LT63"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010999"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011126.1">
        <property type="protein sequence ID" value="ENSXCOP00000010999.1"/>
        <property type="gene ID" value="ENSXCOG00000008308.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114160352"/>
    <dbReference type="GeneTree" id="ENSGT00390000006617"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026120">
        <property type="entry name" value="TMEM11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15099">
        <property type="entry name" value="PROTEIN PM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15099:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 11, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14972">
        <property type="entry name" value="Mito_morph_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="187" mass="21004" checksum="6071D162777530DD" modified="2018-12-05" version="1">MASLGRRRGVPVSRERAVMADSNCYIVHEIYNGESAQDQFEYELEQALEAQCKYIVIEPTRMGDETARWITVGNCLHKTAVLSGTVCLLTPLSLPIEYSRYVALPAGALSVACAALYGISWQFDPCCKYQVEYDSQKLSRLPLHTLTSSTPVVLVRRDDVHRKRLHNTIALAALAYCVKKIYELYAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LU69</accession>
    <name>A0A3B5LU69_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Paired domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LU69"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015548"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015741.1">
        <property type="protein sequence ID" value="ENSXCOP00000015548.1"/>
        <property type="gene ID" value="ENSXCOG00000011755.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165785"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048593">
        <property type="term" value="P:camera-type eye morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030902">
        <property type="term" value="P:hindbrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00131">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000003">
        <property type="entry name" value="Paired box protein Pax-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000069">
        <property type="entry name" value="Paired box protein Pax-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001523">
        <property type="entry name" value="Paired_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043565">
        <property type="entry name" value="PAX_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636:SF41">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-6-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00292">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00027">
        <property type="entry name" value="PAIREDBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00351">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51057">
        <property type="entry name" value="PAIRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0563">Paired box</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Paired" evidence="10">
        <location>
            <begin position="4"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="163" mass="17709" checksum="22D3C02BF3D4733E" modified="2018-12-05" version="1">LHVGHSGVNQLGGVFVNGRPLPDSTRQKIVELAHSGARPCDISRILQTHDEVQVLDSEKVSNGCVSKILGRYYETGSIRPRAIGGSKPRVATPEVVAKIAQYKRECPSIFAWEIRDRLLSEGICTNDNIPSVSALTGSCVHDLICHMNLSLSLSVLSGFSRVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVB6</accession>
    <name>A0A3B5LVB6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phosphodiesterase</fullName>
            <ecNumber evidence="16">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:25278"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>3',5'-cyclic GMP + H2O = GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16957"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:16958"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a nucleoside 3',5'-cyclic phosphate + H2O = a nucleoside 5'-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14653"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57867"/>
            <dbReference type="ChEBI" id="CHEBI:58464"/>
            <dbReference type="EC" id="3.1.4.17"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:14654"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="16">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVB6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014810"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014996.1">
        <property type="protein sequence ID" value="ENSXCOP00000014810.1"/>
        <property type="gene ID" value="ENSXCOG00000011225.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159336"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047555">
        <property type="term" value="F:3',5'-cyclic-GMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000008">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0114">cAMP</keyword>
    <keyword evidence="4" id="KW-0140">cGMP</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="193"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="PDEase" evidence="19">
        <location>
            <begin position="590"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="381"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="475"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="951"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1016"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="401"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="478"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="497"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="953"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1021"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1040"/>
            <end position="1049"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1049" mass="117314" checksum="2A3773BEB752F23B" modified="2018-12-05" version="1">MAAREEGEVLHGKLRHHDGTGSVNSSEGFRNGYVKSCVTPLKQDHQRGFLFSVCRFCNEDVLNSKLLKVSALYVGAFVVFAVSFLISRSLQSDSVWDLRTFLLGFSQSVSPLFSIGCAFFFLTFYLRRKKKQSSRSWLLSLPASCYLGDFLVLRFLQWGEDQHQMQMVGRLLFVLGCVGLLTLIPTLKLKHSVLVLVFASVVWLLSLTSLSGLPALLRPLLACFAGVSGSLLGICFDRFYQTREAPCGISSAEEKVPVIKPRRRSSCVSLGETSTSYYGSCKMPRRPSLPCISREQMILWDWDLKHWYKPQYQVMSGNGVDLSVINEARNLVSELLMDSSLSPNTAATLRSISSLMGTFSGSCRPRVNPFTPFPGFYSVEAEDSAERSDRKGMKGLSSRNSLPTPQPRRSSTSSTPSALPPLDSASSRWERNNGKRPLCDLSLTSSSLTNGPNANLLTIPKQRSSSVTLTYHAVPRRTGTSPSLSPVTSPSYSPPTLNTGSSSSLTPRLPVEFPDTADFLTKPSVNLNLHKPLVSSTDFQQAFRSSTFSLCTSCGRQMLKGISSSESGDSHQLLNSELFLLFMCLLCGCLQGEQEFRLDSMLEDHEGLMEKMDTWNFQIFELVDTTGGKTGRILSYVTYTLFQDMCLFEIFKIPVKEFMSYFCALESGYRDIPYHNRVHATDVLHAVWYLTTRPIPGFQQIHSEHVTGSDTDSGISPGRISYATSKSSSISDDSYGCLAWNIPALELMALYVAAAMHDYDHPGRTNAFLIATNAPQAVLYNDRSVLENHHAASAWSLYLSQPEYNFLANLDHVEFKRFRFLVIEAILATDLKKHFDFLAEFNAKVNDVNSPGIDWTNENDRLLVCQVCIKLADINGPAKVRDLHLKWTEGIVNEFYEQGDEEARLGLPISPFMDRSAPQLAKLQESFITHIVGPLCNSYDAAGLLPGHWVNESESDEEDDEAQEDTDEEEDEEDELEVDLGTGRTKGRRRLFSCIMQHLTENHKVWKKIIEEEDKNKELGKQQQPDSLPTSPSDDIQVIQEEEEGEERQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWA0</accession>
    <name>A0A3B5LWA0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">BCL2 interacting protein 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NIP3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027862322.1">
        <property type="nucleotide sequence ID" value="XM_028006521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWA0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012119"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012257.1">
        <property type="protein sequence ID" value="ENSXCOP00000012119.1"/>
        <property type="gene ID" value="ENSXCOG00000009163.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114137730"/>
    <dbReference type="KEGG" id="xco:114137730"/>
    <dbReference type="CTD" id="568155"/>
    <dbReference type="GeneTree" id="ENSGT00390000013415"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097345">
        <property type="term" value="P:mitochondrial outer membrane permeabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010548">
        <property type="entry name" value="BNIP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15186:SF4">
        <property type="entry name" value="BCL2_ADENOVIRUS E1B 19 KDA PROTEIN-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15186">
        <property type="entry name" value="RE48077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06553">
        <property type="entry name" value="BNIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="191" mass="21359" checksum="94F24EA5883DCDA9" modified="2018-12-05" version="1">MSLQKDASSDESLQGSWVELHFSGTGSQGTSHRGSQEHIPTSSQEGDVEKMLLEAQHESGRSSSKGSSQCNSPLRSQTPLLLWRGSEGNSSQSDEDFQERRREVERLMKKNADWIWDWSSRPENSPPKEFLLKYPKHSTSLSIRNTSVMKKGGILSADFLKIFLPSLIISHILALGLGIFIGKRLTSHNTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYA0</accession>
    <name>A0A3B5LYA0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Target of myb1 membrane trafficking protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the TOM1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYA0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016132"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016336.1">
        <property type="protein sequence ID" value="ENSXCOP00000016132.1"/>
        <property type="gene ID" value="ENSXCOG00000012191.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156865"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14233">
        <property type="entry name" value="GAT_TOM1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000003">
        <property type="entry name" value="TOM1-like protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014645">
        <property type="entry name" value="TOM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13856:SF32">
        <property type="entry name" value="TARGET OF MYB1 MEMBRANE TRAFFICKING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13856">
        <property type="entry name" value="VHS DOMAIN CONTAINING PROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036948">
        <property type="entry name" value="TOM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VHS" evidence="6">
        <location>
            <begin position="20"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="GAT" evidence="7">
        <location>
            <begin position="217"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="160"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="445"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="163"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="196"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="463"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="477" mass="53492" checksum="658B5B00253349F0" modified="2018-12-05" version="1">MEFLLGNPFSTPVGQRIDRATSGSLESEDWGLNMEICDIINETDEGPRDAVKAIKKRIVGNKNFREIMLALTVLETCVKNCGHRFHVLVASQEFVEGVLVRSILPKYNPPTALHDRVLSLIQSWADAFRSSPSLVGVVYVYDDLRRRGLEFPMTDLDALSPIHTPNRSIPENGTPEETSVAAPTRPSPEQVPAAAPTSQNTSSPVQVSNGPVPLTPEQEQKLRSELALVKGNLAVMSQMLNELKPGQSTADDADLLQQLFSVCKKMQSRVVELIPQLVDEGFMEELLVVNDDLNNTFIRFERFERLSKAQSTNTQQVKIIRSCLINLSVLFEMKQDIICFYHLKFCFAEVEEEFDMFAQTRGSSLAEQRKSVKYEDPEMVEGLAEALDSRLQVTGGVCTPRRHSSNLQEDHHLELKTISEGLHKTHIFLEFDKFLDDRAKAADHSNLSIRNVPPSTPRPQAQPAKQQDATHDQLFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYY6</accession>
    <name>A0A3B5LYY6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rho GTPase-activating protein 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Rho-type GTPase-activating protein 19</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYY6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016366.1">
        <property type="protein sequence ID" value="ENSXCOP00000016160.1"/>
        <property type="gene ID" value="ENSXCOG00000012211.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157331"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04392">
        <property type="entry name" value="RhoGAP_ARHGAP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000022">
        <property type="entry name" value="rho GTPase-activating protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047941">
        <property type="entry name" value="ARHGAP19_RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14963">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN 18,19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14963:SF7">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="5">
        <location>
            <begin position="102"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00055252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00070235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00083391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="400" mass="45411" checksum="A480D81151953BED" modified="2018-12-05" version="1">MLAATKSTEQNTNCSVFLCHEKPDANQPVIFNPDFFVERFRHEHPQIFADLVLSNITRLIDLPGDEFAQLTGELVPKVPTSTGFLRSFNFRKRKEKGVIFGAPLTEEGIAQICQLIEYLSKNLHVEGLFRVPGHSLRQAALREMLNNGTEIDLETGDFHPNDAATLLKVFLGELPEPLLMHRHYHAHLKIGELTCFDEKGNKTNVPDKARQIEAFQLLFMLLPPTNRSLLKLLLDLLYHTARSQNMNKMSAINLATMFAPHIIWPKNVTASDLQGNIEKLNNGVAFLIRHSQKMFKAPVYIKEYARLFYTGSKTLQSNVSMSIAGETSSTSNIKPSENQSYTESCLRELYKQVNSMPESAKKKKLIRRTRTPFIRRHWRSRSVGGIIKVGGRAFIILHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0N3</accession>
    <name>A0A3B5M0N3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuolar protein sorting-associated protein 53 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the VPS53 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0N3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016962"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017181.1">
        <property type="protein sequence ID" value="ENSXCOP00000016962.1"/>
        <property type="gene ID" value="ENSXCOG00000012788.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015165"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007041">
        <property type="term" value="P:lysosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.110:FF:000001">
        <property type="entry name" value="vacuolar protein sorting-associated protein 53 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.110">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 53, C-terminus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039766">
        <property type="entry name" value="Vps53"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031745">
        <property type="entry name" value="Vps53_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038260">
        <property type="entry name" value="Vps53_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007234">
        <property type="entry name" value="Vps53_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12820:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 53 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12820">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16854">
        <property type="entry name" value="VPS53_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04100">
        <property type="entry name" value="Vps53_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vps53 N-terminal" evidence="13">
        <location>
            <begin position="95"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Vps53 C-terminal" evidence="14">
        <location>
            <begin position="663"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="344"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="762"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="368"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="768"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="801" mass="90821" checksum="4480B2C62405F69E" modified="2018-12-05" version="1">MMEEEEFEFTEDLEAILHLTPEVQLAIEQVFPSQDPLDRADFNAVEYINTLFPTEQSLANIDDVVNKIRLKIRRLDDDIRTVVRGQTNVGQDGRQVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRKRQYGEVANLLQGVVNVLEHFQKYMGIPQIRQLSERVKAAQSELGTQILADFEEAFPAQGSKRPGGPSNVLRDACLVANVLDPRIKQEIIKKFIRQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPEEWCMTERIAVEFCHITKVELAKVMRTRAKEIEVKLLLFAIQRTTNFEGLLAKRFTGCTLTDIPGVRKRPDSPLDQTNPFLEDDPGEDVGSEKDEDLPRKPKAPDNPFHGIISKCFEPHLYVYIESQDKNLGELIDRFVADFRAQGPPKAGTEEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKFLREYAWKILSGNLPKSSSNSGGLTITSLLKEKEGYEAAKFNVDELCLICSILSTAEYCLATTQQLEEKLKEKVDKILVERINLTGEMDTFSTVISNSIQLLVQDLDAACDPALSAMSKMPWQSVEHVGDQSPYVTSIIMHIKQNVPIIRDNLASTRKYFTQFCIKFTNSFIPKFINHLFRCKPISMVGAEQLLLDTHSLKTVLLDLPSIGSQVLRKAPASYTKIVVKGMTRAEMILKVVMAPHEPAVVFVDNYIKLLADGNPETFQKILDMKGLKRSEQSSMLELFRQRLPTPPSGSDSGPSLSFSAPTPEQESSRIRKLEKLIKKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3J2</accession>
    <name>A0A3B5M3J2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Kelch-like ECH-associated protein 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting nfe2l2/nrf2 for ubiquitination. Keap1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR(KEAP1) complex mediates ubiquitination and degradation of nfe2l2/nrf2, a transcription factor regulating expression of many cytoprotective genes. In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting nfe2l2/nrf2 nuclear accumulation and expression of phase II detoxifying enzymes.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the KEAP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3J2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018032"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018259.1">
        <property type="protein sequence ID" value="ENSXCOP00000018032.1"/>
        <property type="gene ID" value="ENSXCOG00000013584.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159543"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071379">
        <property type="term" value="P:cellular response to prostaglandin stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071466">
        <property type="term" value="P:cellular response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030536">
        <property type="term" value="P:larval feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18458">
        <property type="entry name" value="BACK_KLHL19_KEAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18248">
        <property type="entry name" value="BTB_POZ_KLHL19_KEAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000024">
        <property type="entry name" value="Kelch-like ECH-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000001">
        <property type="entry name" value="Kelch-like family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kelch-type beta propeller"/>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
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    </dbReference>
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        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047098">
        <property type="entry name" value="KEAP1_BACK"/>
    </dbReference>
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        <property type="entry name" value="KEAP1_BTB_POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF13">
        <property type="entry name" value="KELCH-LIKE PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="BTB"/>
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    </dbReference>
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        <property type="entry name" value="Kelch_1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="11">
        <location>
            <begin position="48"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="604" mass="67011" checksum="F85983546AD45D40" modified="2018-12-05" version="1">MTECLTECKALVTPSTRNGHRVFSYTLESHTSAAFAIMNELRLEKQLCDVTLRVRYKDLEAVDFVAHKVVLASSSPVFRAMFTNGLKECGMELVPIEGIHPRVMDRLIEFAYTASISVGEKCVIHVMNGAVMYQIDSVVKACCDFLVQQLDPSNAIGIASFAEQIGCTELHQKAREYIYMNFSQVATQEEFFNLSQCQLVNLISRDELNVRCESEVFQACVAWVRYDHENRRPYVQALLQAVRCHSLTPNFLQAQLQSLDWDPQCKDYLAQIFQDLTLHKPTKAVPCRTPKVPQLIYTAGGYFRQSLSYLEAYNPCTGAWLRLADLQVPRSGLASCVISGLFYAVGGRNNAPDGNMDSNTLDCYNPMNNCWHPCAPMSVSRNRIGVGVIDGMIYAVGGSHGYDPERDQWQLVAPMLTRRIGVGVAVINRLLYAVGGFDGANRLSSCECYYPEKDEWRTMAPMNTVRSGAGVCALGNQIFVMGGYDGTNQLNTVERYDVETDTWSSAASMRHRRSALGVTALHGRIYVLGGYDGNTFLDSVESYDPETDSWSEVTHMTSGRSGVGVAVTMEPCQKGLSQSQMTESFEGSIVQSVSSDFSQCGPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3Z7</accession>
    <name>A0A3B5M3Z7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DRBM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSXCOP00000016146.1"/>
        <property type="gene ID" value="ENSXCOG00000012203.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="dsRBD_dom"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DRBM" evidence="3">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="67"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="154" mass="16984" checksum="300AA382C08A484E" modified="2018-12-05" version="1">MRLNQYQSRLEYRLISQTGPVHEPVFTMSVDVKGKTYEASGPSKRAAKLNVAIKVKLINLLYISEVKSSSTYSSPPVPSTSDTKGQSYRSMPPPVDQQSPYSYGYGEEKKKMLTQSNPVVQGGNSSAYSTAYPSSVTAGHSYDYGNNRLLLRCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4B3</accession>
    <name>A0A3B5M4B3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4B3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016467.1">
        <property type="protein sequence ID" value="ENSXCOP00000016261.1"/>
        <property type="gene ID" value="ENSXCOG00000012290.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
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    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784:SF8">
        <property type="entry name" value="C-TYPE MANNOSE RECEPTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="45"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="164" mass="19888" checksum="2479E49046724FDA" modified="2018-12-05" version="1">MFKSCHRFFKVFSLDVIKKVQFWFYDKLQIIFLMLSFQQCLYDQHNFFCVNMVPVAEKKTWEDALDHCREKNTNLTSLLSLTESFMAQSEIKSSDYTEQVWIGLRYLGNSWMWVNGDPLPYEAWPNGDQDHINFKIQEELWIFLEKRKRFHIRTMTSPAKHRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4F0</accession>
    <name>A0A3B5M4F0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Voltage-gated hydrogen channel 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Hydrogen voltage-gated channel 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4F0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018481.1">
        <property type="protein sequence ID" value="ENSXCOP00000018250.1"/>
        <property type="gene ID" value="ENSXCOG00000013745.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159403"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030171">
        <property type="term" value="F:voltage-gated proton channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010043">
        <property type="term" value="P:response to zinc ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000054">
        <property type="entry name" value="voltage-gated hydrogen channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031846">
        <property type="entry name" value="Hvcn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46480">
        <property type="entry name" value="F20B24.22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46480:SF1">
        <property type="entry name" value="VOLTAGE-GATED HYDROGEN CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="14">
        <location>
            <begin position="57"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="207" mass="23861" checksum="7345C990EEF7EFBD" modified="2018-12-05" version="1">MSRYLRHFTAVGDVHPGQWVEEELHVDSEELSPATGQYPAAPTFRASLKRLYTSDRFQIAVVCLVVLDAIFVMAELLIDLSVIKLEHGHLAPEVFHYLSLALLTFFMVELAGKLFVFRLEFFHHKFEVFDGLVVVVSFVLDIVFIFHEDAFDGVGLLILLRLWRVARIVNILVSVKTRADQKIHKLKESYDHLVERATELQKRNDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4Z6</accession>
    <name>A0A3B5M4Z6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transportin 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4Z6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019238"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019475.1">
        <property type="protein sequence ID" value="ENSXCOP00000019238.1"/>
        <property type="gene ID" value="ENSXCOG00000014460.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156708"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="7">
        <location>
            <begin position="37"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="331"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="357"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="882" mass="99620" checksum="A96250E86768D8F8" modified="2018-12-05" version="1">MCYLHRMEWQPDEQGLQQVLQLLKDSQSPDTATQRAVQEKLEQLNQFPDFNNYLIFVLTSLKSEDEPTRSLSGLILKNNVKAHYQNFPPNVADFIKRECLNNIGDPSPLIRATIGILITTIASKGELQTWPELLPQLCNLLNSEDYNTCEGSFGALQKICEDSSELLDSDALNRPLNIMIPKFLQFFKHCSPKIRSHAIACVNQFIIGRAQALMDNIDTFIESLFALAGDEDSEVRKNVCRALVMLLEVRIDRLIPHMHSIIQYMLQRTQDPDENVALEACEFWLTLAEQPICKEALSGHLVQLIPILVNGMKYSEIDIILLKGDVEEDDTIPDSEQDIKPRFHKSRTVTLQHEGGEGEEGEDIDEDEDDDDDTLSDWNLRKCSAAALDVLANVFREELLPHLLPLLKGLLFHPDWVIKESGILVLGAIAEGCMQGMVPYLPELIPHLIQCLCDKKALVRSIACWTLSRYAHWVVSQPPDAHLKPLMTELLKRILDGNKRVQEAACSAFATLEEEACTELVPYLSFILDTLVFAFGKYQHKNLLILYDAIGTLADSVGHHLNQPEYIQKLMPPLIAKWNELKDEDKDLFPLLECLSSVATALQSGFLPYCEPVYQRCVTLVQKTLAQAMMYSQQPDQYEAPDKDFMIVALDLLSGLAEGLGGHVDTLVARSNIMTLLFQCMQDTMPEVRQSSFALLGDLTKACFPHVKPCIAEFMPILGTNLNPEFISVCNNATWAIGEICMQMGVEMQPYIAMVLNQLVEIINRPNTPKTLLENTAITIGRLGYVCPQEVAPMLPQFIRPCCEKTVEICCSLRKTLPFVGFDFIFFCDAVASWVNPKDDLRDMFYKILHGFKEQVGEENWQQFSEQFPPLLKERLAACYGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6K4</accession>
    <name>A0A3B5M6K4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cdk5 and Abl enzyme substrate 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6K4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019065"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019302.1">
        <property type="protein sequence ID" value="ENSXCOP00000019065.1"/>
        <property type="gene ID" value="ENSXCOG00000014329.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00400000022086"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000020">
        <property type="entry name" value="CDK5 and ABL1 enzyme substrate 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012388">
        <property type="entry name" value="CABLES1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22896">
        <property type="entry name" value="CDK5 AND ABL1 ENZYME SUBSTRATE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22896:SF4">
        <property type="entry name" value="CDK5 AND ABL1 ENZYME SUBSTRATE 2-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025798">
        <property type="entry name" value="Cables"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="7">
        <location>
            <begin position="285"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="43"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="396" mass="43781" checksum="30828AEEDDFA0DD1" modified="2018-12-05" version="1">MATALCGLQSSNGKQTRARREHRRKTKDSLRRRAAIFFLSNISLDGRPQGQPTAGNADQIAGEETGLGDGEGGAAVKPLRTDSGGPVNQVTDPAAEPGSGSSFSLPGALGFVRPSNISGSPGPKVPKKVHFIKSMRQYDTRGSRIMLICTKRSLYAAFSVLPYGERAHLSDPKLDAQRQRLSSVVAADLLPSLEGVELGATGKVSIQPYIQPYNQPTILTNIQPYNQPSITQPVCIELLVYFPSLQTTIIEYVKPSDLKKDMNETFKEKFPHIKLTLSKIRSLKREMRAVSEECGLQPVTIAMAFVYFEKLVLQGRLNKQNRKLVAAACVLLAAKISSDLRKPEVKQLIDKLEECFRINRRELIPLEFPVLVALEMGLYLPESKVMPHYRRLVQQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7L5</accession>
    <name>A0A3B5M7L5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lipocalin/cytosolic fatty-acid binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 9">Belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7L5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020511.1">
        <property type="protein sequence ID" value="ENSXCOP00000020263.1"/>
        <property type="gene ID" value="ENSXCOG00000015198.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047107"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019841">
        <property type="term" value="F:retinol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034632">
        <property type="term" value="F:retinol transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000005">
        <property type="entry name" value="Retinol binding protein 4, like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022271">
        <property type="entry name" value="Lipocalin_ApoD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022272">
        <property type="entry name" value="Lipocalin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002449">
        <property type="entry name" value="Retinol-bd/Purpurin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11873:SF1">
        <property type="entry name" value="PURPURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11873">
        <property type="entry name" value="RETINOL-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036893">
        <property type="entry name" value="Lipocalin_ApoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00179">
        <property type="entry name" value="LIPOCALIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01174">
        <property type="entry name" value="RETINOLBNDNG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00213">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0683">Retinol-binding</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017107053" description="Lipocalin/cytosolic fatty-acid binding domain-containing protein" evidence="7">
        <location>
            <begin position="22"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="10">
        <location>
            <begin position="40"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="23"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="89"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR036893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036893-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="195" mass="21749" checksum="64B7AB80EDD1B2C6" modified="2018-12-05" version="1">MDFQLLGLVMLFLACVEQSLASCVVDRFTVKQDFDPQRYAGKWYALQKKDPEGLFLQDNISAEYTVADDGAMVASSRGRVTLFGFWVVCADMAAQYSVPDPNTPGKMFMNYQGLASYLSSGGDNYWIIDTDYDNYAITYACRTLKEDGSCEDGYALVFSRNPRGLPPAIQRVVRQKQEEICMAGQFQPVLQSGAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8A9</accession>
    <name>A0A3B5M8A9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BMERB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M8A9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016014"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016213.1">
        <property type="protein sequence ID" value="ENSXCOP00000016014.1"/>
        <property type="gene ID" value="ENSXCOG00000012096.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022735">
        <property type="entry name" value="bMERB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF51">
        <property type="entry name" value="[F-ACTIN]-MONOOXYGENASE MICAL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF3585"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BMERB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BMERB" evidence="3">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="116" mass="13969" checksum="121382E7E819A0D6" modified="2018-12-05" version="1">MGKKDDPKLMQEWFKLVQEKNALVRYESELMIARELELEDRQSRLQQELRERMAVEHLKTEKELAQEKQILNEMLEVVEQRDALVALLEEQRLREREEDKDLEAVMLSKGFNLNWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9D5</accession>
    <name>A0A3B5M9D5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin-conjugating enzyme E2 variant 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027876742.1">
        <property type="nucleotide sequence ID" value="XM_028020941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9D5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020125"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020372.1">
        <property type="protein sequence ID" value="ENSXCOP00000020125.1"/>
        <property type="gene ID" value="ENSXCOG00000015086.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114146652"/>
    <dbReference type="KEGG" id="xco:114146652"/>
    <dbReference type="CTD" id="7336"/>
    <dbReference type="GeneTree" id="ENSGT00740000115534"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070534">
        <property type="term" value="P:protein K63-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd23807">
        <property type="entry name" value="UEV_UBE2V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000012">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 variant 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="45"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="180" mass="20150" checksum="25FA104BCDCD06E0" modified="2018-12-05" version="1">MQCAGPPVCCCCCAFLGATRSDVHTSVCFWTRLKKMAASSGVKVPRNFRLLEELEEGQKGEGDGTVSWGLEDDEDMTLTRWTGMIIGPARTNYENRIYSLKVECGPRYPETAPTVRFVTKISMNGINNSNGTVDSRSIPILAKWQNSYSIKIVLQELRRLMMSKENMKLPQPPEGQTYST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MA84</accession>
    <name>A0A3B5MA84_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">palmitoyl-protein hydrolase</fullName>
            <ecNumber evidence="2">3.1.2.22</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:19234"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MA84"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020477"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020727.1">
        <property type="protein sequence ID" value="ENSXCOP00000020477.1"/>
        <property type="gene ID" value="ENSXCOG00000015342.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164487"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101005">
        <property type="term" value="F:deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF41">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE 2-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="7">
        <location>
            <begin position="14"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="176"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="209" mass="23402" checksum="33CD1C163902865D" modified="2018-12-05" version="1">MFGRLTARFGSLRRPMDLNMIDMPRYWINEYTSNLGIGVFHSGIEIYGREFAYGGHPYPFSGIFEINPGNAAELGETFKFKEAIVLGTTDFTEEDIDKIMEELGKEFKGNAYHLMHKNCNHFSSSLSELLCGREIPRWVNRLAYFSSCIPFLQSCLPKEWLTPAALQSHISLGLHKEEQNDSSDEDPTSPSGAAAAGSGSSHSCRHTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MB01</accession>
    <name>A0A3B5MB01_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MB01"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016884"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017101.1">
        <property type="protein sequence ID" value="ENSXCOP00000016884.1"/>
        <property type="gene ID" value="ENSXCOG00000012732.1"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
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        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="255" mass="30042" checksum="9BF17662ED2BF5AD" modified="2018-12-05" version="1">MGRRSSDSEDDSRSRRKRKQRRRSSSSSSSSSSGSRVRSSRSRRSTRRSRSRSKDRRRRSSSSSSQSSSRRRRSRSADRDKGRSHRSHRSRSRERSHSRHRRSRSRSNSRGHRTSHSRKRSRSNSRERDRSRRKGRDRDREKDRERDRGRDRDKDKSKDKKDSGSIKDKLEHLGTSLEEQVRRIKDIEAIESDSFVPQAFKSSRDDTKLPMSIVYDDSGTLAHPSLFMDKERAEDLWLSRLISLRQERLMGSPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBX2</accession>
    <name>A0A3B5MBX2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBX2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021366.1">
        <property type="protein sequence ID" value="ENSXCOP00000021107.1"/>
        <property type="gene ID" value="ENSXCOG00000015791.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="30433" checksum="2A7502176E492676" modified="2018-12-05" version="1">MPSIGSTCPLLAVSCLTVSDCVQFESDFHLLELILLAEASKHKPLINAFQVKCRSQQRKQAKKVRKLDLEKAVGSHKAVCNRAVVAERTFIYLCSKLWPALEQQHPTFRHFVPLKNTVATALWKLATGSDYRSVGQLFGVSIMTTCICAQEFCAAGETLLVPEQVRLPLCVGAIIVNIGKGLCWNVLAGLGILHTAKVLRLSTQNIGTVTPSYYTLDDSMCNENVCSPRVVVENAFGWLKGRWKMDSDVQLVRITALTFLPQENQQQVTRPETGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MC30</accession>
    <name>A0A3B5MC30_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Purine nucleoside phosphorylase</fullName>
            <ecNumber evidence="4 14">2.4.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Inosine-guanosine phosphorylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Preferentially acts on 6-oxopurine nucleosides including inosine and guanosine.</text>
    </comment>
    <comment type="function">
        <text evidence="14">The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 14">
            <text>2'-deoxyguanosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:27738"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17172"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>2'-deoxyinosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27750"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:28997"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>guanosine + phosphate = alpha-D-ribose 1-phosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:13233"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:16750"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 14">
            <text>inosine + phosphate = alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27646"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 14">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the PNP/MTAP phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.1" evidence="4 14"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MC30"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018831"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019067.1">
        <property type="protein sequence ID" value="ENSXCOP00000018831.1"/>
        <property type="gene ID" value="ENSXCOG00000014177.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182991"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047975">
        <property type="term" value="F:guanosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004731">
        <property type="term" value="F:purine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09009">
        <property type="entry name" value="PNP-EcPNPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1580:FF:000004">
        <property type="entry name" value="Purine nucleoside phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011270">
        <property type="entry name" value="Pur_Nuc_Pase_Ino/Guo-sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011268">
        <property type="entry name" value="Purine_phosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018099">
        <property type="entry name" value="Purine_phosphorylase-2_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01700">
        <property type="entry name" value="PNPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01697">
        <property type="entry name" value="PNPH-PUNA-XAPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006054">
        <property type="entry name" value="PRK08202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904">
        <property type="entry name" value="METHYLTHIOADENOSINE/PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904:SF13">
        <property type="entry name" value="PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000477">
        <property type="entry name" value="PurNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01240">
        <property type="entry name" value="PNP_MTAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="16">
        <location>
            <begin position="10"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="67"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>a purine D-ribonucleoside</name>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>a purine D-ribonucleoside</name>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000477-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="277" mass="30013" checksum="E5E6B866A7E2AE87" modified="2018-12-05" version="1">MSHTKHRPIVAIICGSGLGMLADTLKCQDSFDYSDIPGFPQSTVQGHAGRLVFGELKGKSCVCMQGRFHMYEGHSLLKTTFPIRVFKLMGVETLIVTNAAGSLADSLRPGDIMIIKDHINFPGLVGLNPLCGPNDEKFGPRFPAMSGCYDKGLRSLMMELAKQMGVSSLVQEGVYAMVGGPNFETIAEAKLLHRLGVDAVGMSTAPEVVVATHCGLRVFGLSLITNKVVKSYEDSESVNHEGVLEVGRLRSQTVQQLVTELISRMEINNNENTNNVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDX3</accession>
    <name>A0A3B5MDX3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Kainate-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Postsynaptic cell membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDX3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018113.1">
        <property type="protein sequence ID" value="ENSXCOP00000017889.1"/>
        <property type="gene ID" value="ENSXCOG00000013477.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163942"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13685">
        <property type="entry name" value="PBP2_iGluR_non_NMDA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000143">
        <property type="entry name" value="Probable glutamate receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000364">
        <property type="entry name" value="Si:dkey-183j2.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000398">
        <property type="entry name" value="Si:dkey-183j2.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017466065" description="Kainate-binding protein" evidence="18">
        <location>
            <begin position="27"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="19">
        <location>
            <begin position="20"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="20">
        <location>
            <begin position="28"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00082164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="376" mass="41566" checksum="9EF910EF260A6A46" modified="2018-12-05" version="1">VNRARMLFAFLLLSSGLFLDLEVCTQDPYTMSKGGQLEGFCMDLLSEVAKTVGLKYKVKLVKDGAYGRQDESGNWNGMIGEVVRGEADLAIAPLTLTAAREKVVGMTKPFMQTGISILLRKDISEGAGFFHFLSPFAPETWMGILIAYMATAASIFIAARVSPCEWSEAQSERNRFSFLYSLWYAAGALTLQGVGPHPKALSGRVICSSWWLFSVVLLACYFSNLSSSKTSESSHLTVKGFEDLAGQNTIEYGCLAGSSTLAFFKNSNNPVYRRIYEHMERTQSFVSSMDEGVKQAKEGNFAFIGESVSLDLAVARHCELVRVHEVIGMRGYSIAAPLGKEPPLKMSFILIYQMLIKYKTFYNNKVTYCASLNRLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5ME12</accession>
    <name>A0A3B5ME12_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger protein 362a</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5ME12"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017919"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018143.1">
        <property type="protein sequence ID" value="ENSXCOP00000017919.1"/>
        <property type="gene ID" value="ENSXCOG00000013503.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156275"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000233">
        <property type="entry name" value="Putative zinc finger protein 362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000158">
        <property type="entry name" value="Zinc finger protein 362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000377">
        <property type="entry name" value="zinc finger protein 362 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000216">
        <property type="entry name" value="Zinc finger protein 384 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381:SF401">
        <property type="entry name" value="ZINC FINGER PROTEIN 384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="238"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="294"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="324"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="354"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="49"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="114"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="50"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="404" mass="44229" checksum="62D3354D4D703E14" modified="2018-12-05" version="1">MAEPRFNNPYFWPPPPSMPGQLDNLVLINKIKEQLMAEKIRPPHLPAVSAASQQPLMSPSGQAEGSQHGMSKAQQGSGLHSPSPLQPDIALHARPASSSVTGVALASRTGNLSTSEIITPTTPTSSSHSRLGGAPTPHLISGLAGSHGIEPGKTNGGFVGLLGPPPKQERGRKKIKAENGSSLLVVPYPILASGNDQSCVTITTKEGKTYRCKVCPLTFFSKSDMQIHSKTHTEAKAHKCPHCTKTFANASYLAQHLRIHLGLKPYRCSYCERCFRQLSHLQQHTRIHTGDRPYKCLHPGCEKAFTQLSNLQSHQRQHNKDKPFKCSNCFRAYSDSASLQIHLSVHAIKNAKAYCCSMCGRAYTSETYLMKHMPKHAMVEHVMSHHSPQNRTGSPNIPIRISLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MF39</accession>
    <name>A0A3B5MF39_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MF39"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023179.1">
        <property type="protein sequence ID" value="ENSXCOP00000022903.1"/>
        <property type="gene ID" value="ENSXCOG00000017110.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244823"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF381">
        <property type="entry name" value="TRACE AMINE ASSOCIATED RECEPTOR 19P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="269" mass="29927" checksum="AAC1460E6E7DDB5F" modified="2018-12-05" version="1">SRQLHTPTNILLLSLAVSDFLVGFLIVFQILLIDGCWYLGDFMCVLYYIIDYVITSASVENMVLISVDCYVAVCNPFQYPTTVTQKRVCVCVSLCWMFSAVCVFIVFKENLGQPGRFNSCIGECVVGFDKTAGIIDILFFFIGPVTIIIILYVRVFVVAVSQARAMRVAAVSLQGSVRGNAKKSELKSARTLGVVAAAFLICICPYFFLSVTGGETQFSASSFAFFLFYLNSCLNPLIYVLFFPWFRKTIKLIFTLQILKAGSSQTNIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MG11</accession>
    <name>A0A3B5MG11_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SET binding protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000022545"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022821.1">
        <property type="protein sequence ID" value="ENSXCOP00000022545.1"/>
        <property type="gene ID" value="ENSXCOG00000016852.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158784"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042800">
        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46147">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE ASH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46147:SF2">
        <property type="entry name" value="SET-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="186"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="570"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="680"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="976"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1020"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1070"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1209"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1280"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="209"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="310"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="330"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="595"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="681"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="976"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="987"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1217"/>
            <end position="1226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1319" mass="145372" checksum="3CCAA3D8FACBEB16" modified="2018-12-05" version="1">MEPRDLVGSARPKEADLRGGRAGPSEEHQLAAGGAGPVHGDDVINGAASEGEGLEEQGEGLMEEQEFSIKEASFQEGSLKLKIQTTKRTKKPPKSLENYICPPEIRMTIRPPAGEGKGGRQGRTGGGAGAGRGPKDEERGPPRKRVSPPLFFLLLLYSLRVSNKSPGRGKGLQEVSEQLFGNIKRKYGRKDSQRIFANPQSADAPWGRQSERELESPANSEERQRYRQEGTAEQFQEAREERRKDEREQANIGRRGDEEERGMPVQAEDGKGRKRRRRPSVDESFSMEERSQQQTLDTPEKPPAGPPEPKIAHKIEAHKSDSRLSSQRDGSAERVEKAEKADRRDKAERAERGEAATCGPDQETDLNSNRVKKNPVGRPKSLTDSLKLREATYNQINKPSLDPPSAFPITPSSPLYTNTDSLTVHAPIKRKRGRPKKQPLLTVETIHEGTSTSPPSPLAKESNPGISRRRKMHTLNPLVHAASSSPSDCFNSLKVKRGRGHPRAVIKTKLGKMQSILNEILSGSSQNGSLALKSASAPVTSAMSAMATTIEARLGKQINVSKRGTIYIGKKRGRKPRAETQGSVALKASRDKPQMSLSMSSFYDSPAVPSTNSSPSSCALSLRASNTDATMPSLQPISALPTKPSGRGFLSGGWKLSPPRLLANSPSHLSEGASVKEVTLSPISESHSEETIPSDSGIGTDNNSTSDQTEKGPASRRRYSFDLCGFESPEAAALDTSTRGSRPRCERKVTAVDSYLSQQEKKQKHHRRKRKCMQTRDHLHFLSELEEVVLKFQQLRVSHRLYTCYPQHPYPSIFRLNFHHYYPLSYDSYACDSGSYLRRSADLKAKRRRGRPAKAGEPITSKLPFVPGYGYALGGGNYYAAPYAMPYAPPLNLGYFPPTPPFYLPHHSLGPAPPSPFMRPAVPPPKAFLSSGHPKLQVGAKIRSANGPLQGPTVRSEALGSLSAGSAGGMAGVRLHKRKHKHKHKHKDEPLLSPRDRQELGGLFSGAKTNVRLGMLSERRDLAGQGSSKHLEKQRGNARGPNLGSGLGIFESDQLSGHALADGQFHSRQTAQPAKGFMSSYGSQSQRSEEASDLFSGLREDDCAGRSRRTRLAVFGDQGLMSFQTARQEPEQMNERSSPALTGKRHIMTFLFVSEEKALTLFDFKQCSMLQRRYKRSEVEQIQKDVRRMHSLNFDHVQKILRAKRLQRQAKTGNNVIKRRPGRPRKQPLEESTLGMPVLERCDNLPGRHSLRPSLALESQELPAHDSIAATIETVVHQARCVPPPGNKEGKRRGRGHSRDQLWAPAPQVLPKDSQLLPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGE2</accession>
    <name>A0A3B5MGE2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGE2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018664"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018899.1">
        <property type="protein sequence ID" value="ENSXCOP00000018664.1"/>
        <property type="gene ID" value="ENSXCOG00000014049.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00720000109813"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF118">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Immunoglobulin V-set" evidence="4">
        <location>
            <begin position="58"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="159" mass="18198" checksum="FB5CA49E4F96B7B6" modified="2018-12-05" version="1">MEELRSWNPPHPLELFTYLLRFVLFTEKTHCKTLNQTAYRTAKTTIKCDYPHMYLSKTKFICKENNFTCKSILTTKSSKNSTGRFTLTHTSSDFNLSISPVSLNDAGVYWCGVIESNGSYQAGISKIQLEIHLFPFPPRCVFPFFLLWCEEASPVCASC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGZ9</accession>
    <name>A0A3B5MGZ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Carboxypeptidase</fullName>
            <ecNumber evidence="7">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of a 32 kDa chain and a 20 kDa chain; disulfide-linked.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGZ9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023027"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023304.1">
        <property type="protein sequence ID" value="ENSXCOP00000023027.1"/>
        <property type="gene ID" value="ENSXCOG00000017209.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00880000138014"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904715">
        <property type="term" value="P:negative regulation of chaperone-mediated autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000335">
        <property type="entry name" value="Carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF502">
        <property type="entry name" value="LYSOSOMAL PROTECTIVE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017106568" description="Carboxypeptidase" evidence="7">
        <location>
            <begin position="26"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="459" mass="51688" checksum="14563EF4E8E1F7D3" modified="2018-12-05" version="1">MSLPVPPQMQVFLWFLLTFLLGAGAAPSADEVSFLPGLQKQPSFRQYSGYLNVADGKHLHYWFVESQNKPSSDPVVLWLNGGPGCSSLDGFLTEHGPFLIANMLYLESPVGVGFSYSDDKNYKTNDTEVSMNNYLALKEFFRLFPEYSSNELYLTGESYGGIYIPTLAERVMEDSSLNLQGVAVGNGMSSYELNDNSLVYFAYYHGLLGSQLWAELQMYCCSGGKCDFHNNQNPNCTEKLSDVQTILYSSGLNIYNLYAPCAGGVPHRFSVDHGQLVVRDMGNIFINHQETRQWNQVLAAVHLSVRLDPPCTNSTPSSLYLNNAYVKKALHISPDALDWAICSSEVNMMYGRLYMDVRKQYLKLLSALKYRVLVYNGDVDMACNFMGDEWFVESLQQQVEVQRRPWVYDGLDGHQIGGFVKEFDNIAFLTVKGSGHMVPTDKPVAAFTMFTRFIKKQPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHV4</accession>
    <name>A0A3B5MHV4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ankyrin repeat, family A (RFXANK-like), 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHV4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023893"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024177.1">
        <property type="protein sequence ID" value="ENSXCOP00000023893.1"/>
        <property type="gene ID" value="ENSXCOG00000017848.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157156"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000031">
        <property type="entry name" value="Ankyrin repeat, family A (RFXANK-like), 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001995">
        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124:SF3">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY A PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50175">
        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="171"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="204"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="5">
        <location>
            <begin position="237"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A2" evidence="6">
        <location>
            <begin position="254"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="303" mass="32830" checksum="4994328870AC13F7" modified="2018-12-05" version="1">MDNLTVAAVEGTAVCPLVAEDMEGICVMPDLSSIKTEQSVGPTPDEPSPQNVAMGIKFILPNRFDMNVCSRFVKSLNEEDSKNIQDQVNSDLEVASVLFKAECNIQTSPSPGIQVRHVYTPSTTKHFSPIKQSTTLTNKHRGNEVSSTPLLILISGVFLCTETVINLQDDEGFTPLMWAAAHGQIAVVEFLLQNGADPNLLAKGRESALSLACSKGYTDIVKMLIDCGVDVNEYDWNGGAPLLYAVHGNHVRCVEILLDSGADPTVESDSGFNAMDMAVAMGHRNVQQVMEAHLLKLLMGIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIH6</accession>
    <name>A0A3B5MIH6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">STEAP3 metalloreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">STEAP3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the STEAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIH6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023425"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023706.1">
        <property type="protein sequence ID" value="ENSXCOP00000023425.1"/>
        <property type="gene ID" value="ENSXCOG00000017490.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008042"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008823">
        <property type="term" value="F:cupric reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051267">
        <property type="entry name" value="STEAP_metalloreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239">
        <property type="entry name" value="DUDULIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239:SF8">
        <property type="entry name" value="METALLOREDUCTASE STEAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ferric oxidoreductase" evidence="6">
        <location>
            <begin position="86"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="250" mass="28587" checksum="FFD32E009FDDB00E" modified="2018-12-05" version="1">MMKSICHRMGFIPVDMGLLSSSLEIENLPLYLFPSWRLPVLCMLLLFVFFYLYNFVRDVLHPFITKHKGAFYKMPIEVVNVTLPSVALVMLALVYLPGLLAAVLQLCSGTKYNRFPNWLDRWLTSRKQLGLCSFLCAVLHAVYSLCLPLRKSARFTLINMAYKQVEMGKEDSWVDEDVWRMELYLSVGIMALGLLSLLAVTSLPSVAGTINWREFSFIQVCFMFSFTSKVISPQRHTIPSVSKTLNVPHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIZ0</accession>
    <name>A0A3B5MIZ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phospholipase A1 member A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1,2-di-(9Z)-octadecenoyl-sn-glycero-3-phospho-L-serine + H2O = 2-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40491"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:74905"/>
            <dbReference type="ChEBI" id="CHEBI:77342"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:40492"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine + H2O = sn-glycero-3-phospho-L-serine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40499"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:64765"/>
            <dbReference type="ChEBI" id="CHEBI:74617"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:40500"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phospho-L-serine + H2O = 2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phospho-L-serine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41187"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:75032"/>
            <dbReference type="ChEBI" id="CHEBI:77830"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:41188"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIZ0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024298"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024589.1">
        <property type="protein sequence ID" value="ENSXCOP00000024298.1"/>
        <property type="gene ID" value="ENSXCOG00000018143.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159279"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008970">
        <property type="term" value="F:phospholipase A1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF111">
        <property type="entry name" value="PHOSPHOLIPASE A1 MEMBER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 16" id="KW-0732">Signal</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017276262" description="Phospholipase A1 member A" evidence="16">
        <location>
            <begin position="20"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="320"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Lipase" evidence="17">
        <location>
            <begin position="20"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="380" mass="42118" checksum="C9C4CCD256AD2CB5" modified="2018-12-05" version="1">MHWEDKAILFSILLVYTSGESQEDGQCAELNNTRWQEYRQQRVEVQYLLLTRKNPDCAQKFDQDSVGKPTSYFNTSRQTKVIIHGFSALGRRPSWVTDLAQALLEAQDANVVVVDWIYGASFAYNLVVENYKEVALQISVLVNQLQRHGCRLESFHLIGVSLGAHVAGFVGTVFEGKIGRITGLDPAGPMFKGADTYDRLDPTDAVFVDAIHTDSDHFGISIPVGHVDFFLNGGMDQTGCARSRFASMYGYVICDHMRALHVYMSALNGTCQLMGIPCASYEDFLKGSCVDCGVFKGRCPAIGKDSPQSLTHAMFKSLDALLYLCIMTLLTPTLILPVFHLCSLCYFILKNTDVCDYQGRIYKDVSPRAGASYGAISTTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJC5</accession>
    <name>A0A3B5MJC5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJC5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024151.1">
        <property type="protein sequence ID" value="ENSXCOP00000023867.1"/>
        <property type="gene ID" value="ENSXCOG00000017830.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="105" mass="11617" checksum="EAFF4069A8BF30D5" modified="2018-12-05" version="1">MTKSPSESEFKYIINPIKPSRFWIRGDPPPQRSAQGCERATRKRETGSWRRDGGRHAGRNAADGMVIVSLKDISSSFPLMIHRNFRDTAEMDVAVGDAAGTQTGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJG2</accession>
    <name>A0A3B5MJG2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma</fullName>
            <ecNumber evidence="9">3.1.4.35</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7 9">Participates in processes of transmission and amplification of the visual signal. cGMP-PDEs are the effector molecules in G-protein-mediated phototransduction in vertebrate rods and cones.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>3',5'-cyclic GMP + H2O = GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16957"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="EC" id="3.1.4.35"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Tetramer composed of two catalytic chains (alpha and beta), and two inhibitory chains (gamma).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the rod/cone cGMP-PDE gamma subunit family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.35" evidence="9"/>
    <dbReference type="RefSeq" id="XP_027897266.1">
        <property type="nucleotide sequence ID" value="XM_028041465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJG2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021261"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021521.1">
        <property type="protein sequence ID" value="ENSXCOP00000021261.1"/>
        <property type="gene ID" value="ENSXCOG00000015913.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114159505"/>
    <dbReference type="KEGG" id="xco:114159505"/>
    <dbReference type="CTD" id="393810"/>
    <dbReference type="GeneTree" id="ENSGT00390000013260"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042622">
        <property type="term" value="C:photoreceptor outer segment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047555">
        <property type="term" value="F:3',5'-cyclic-GMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045742">
        <property type="term" value="P:positive regulation of epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045745">
        <property type="term" value="P:positive regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1120.10:FF:000002">
        <property type="entry name" value="Retinal cone rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1120.10">
        <property type="entry name" value="Retinal cGMP phosphodiesterase, gamma subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006952">
        <property type="entry name" value="PDE6_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037030">
        <property type="entry name" value="PDE6_gamma_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12122">
        <property type="entry name" value="RETINAL CONE RHODOPSIN-SENSITIVE CGMP 3',5'-CYCLIC PHOSPHODIESTERASE GAMMA-SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12122:SF5">
        <property type="entry name" value="RETINAL CONE RHODOPSIN-SENSITIVE CGMP 3',5'-CYCLIC PHOSPHODIESTERASE SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04868">
        <property type="entry name" value="PDE6_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000969">
        <property type="entry name" value="35-cGMP_Pdiase_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0140">cGMP</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="6" id="KW-0844">Vision</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="86" mass="8934" checksum="AC954EE340CA98F7" modified="2018-12-05" version="1">MNASPPAGSALSPGGTAGPTTPKKGPPKFKQRQTRTFKSKAPKPGQKGFGDDIPGMEGLGTDITVVCPWEAFGDMELSDLAKYGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKV4</accession>
    <name>A0A3B5MKV4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">XK-related protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the XK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKV4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021771"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022035.1">
        <property type="protein sequence ID" value="ENSXCOP00000021771.1"/>
        <property type="gene ID" value="ENSXCOG00000016273.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003231"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018629">
        <property type="entry name" value="XK-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051773">
        <property type="entry name" value="XK-related_adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14297">
        <property type="entry name" value="MEMBRANE TRANSPORT PROTEIN XK FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14297:SF4">
        <property type="entry name" value="XK-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09815">
        <property type="entry name" value="XK-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU910716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="414" mass="47799" checksum="9EE09298AFCDE63A" modified="2018-12-05" version="1">MDYHKSNDVIWMSFTITFMLVPAVLIQLTLTFIHRDLGRDRPLVLFLHLLQLGPVIRCFEALVIYFKAGQNEEPYVTLSRKTNLQKGREIELEIGQTERTLATHRNAFKRTAVIQAFLGSTPQLTLQLYATIQEKYILSDRLALMIITLISITYGALVCSVLAIQITYDSIKVRLRPAAYLCMVVWRALEITTRVTTLVLFSTALTHWVILVGALNLLFFFFLPWAEFWARRGSLAEGVENNLSKLGTAVVLCMFTLLYACINVFCWSAVELDLADQELIDRKQSWLRLALYYSGRFLENFVLITLWYFFKSDFYQYVCAPLVVVQLLIGYSVAVLFMLLFYQFCHPCRSLFKFNVHDCLHCVCCRRREEPLELLDPQNCLPPDGAGPLGEREHVMLVDTIMLDTENLHMERKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKY0</accession>
    <name>A0A3B5MKY0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amyloid beta A4 precursor protein-binding family B member 1-interacting protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">APBB1-interacting protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MRL family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKY0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020174"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020421.1">
        <property type="protein sequence ID" value="ENSXCOP00000020174.1"/>
        <property type="gene ID" value="ENSXCOG00000015118.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156105"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01259">
        <property type="entry name" value="PH_APBB1IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000048">
        <property type="entry name" value="Ras association (RalGDS/AF-6) and pleckstrin homology domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000027">
        <property type="entry name" value="Ras association (RalGDS/AF-6) and pleckstrin homology domains 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039664">
        <property type="entry name" value="GRB/APBB1IP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039665">
        <property type="entry name" value="PH_APBB1IP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11243:SF14">
        <property type="entry name" value="AMYLOID BETA A4 PRECURSOR PROTEIN-BINDING FAMILY B MEMBER 1-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11243">
        <property type="entry name" value="GROWTH FACTOR RECEPTOR-BOUND PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-associating" evidence="12">
        <location>
            <begin position="143"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="11">
        <location>
            <begin position="273"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="78"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="471" mass="53724" checksum="C17982FAD2CA7F18" modified="2018-12-05" version="1">CFFQMDDIDAMFSDLLGEMDLLTQVKKLSAILLPFLKKSPLGFMSSLHDLEDNDLDALMADLVADLSETEKKLEAEIQGLKAPPSSSSDLPPPPKGLGLQPASSITSPPSPGNNRSSSEEIEAQMKADKIKLALEKLKEAKVKKLVVKVLMNDGSSKNLMVDERQTVREVLDNLFEKTHCDCNVDWSLCETNHELQLERTFEDHENLVEPLLAWTRDSENKVLFQERKDKFEVFKNPQNFYLWKRDKKTLKDMKDKDKELLVQENFCGTSIIVPDLEGVLYLKEDGKKSWKQRLFQLRASGIYYVPKGKTKSSRDLICFVQFDNVNVYYGKDVKNRYKAPTDFCFVLKHPQIQKESQYIKYLCCDDPWTMNLWVTGIRIAKYGVSLHENFKTAERKAAVSAAMWTNRSTPSSSSPSTPSPTIKGTAARRTLLYLVVMSLHSVDSRFCFLQPKQQLRPTATPPNSSLDRRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLW9</accession>
    <name>A0A3B5MLW9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Vav 3 guanine nucleotide exchange factor b</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLW9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025404"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025711.1">
        <property type="protein sequence ID" value="ENSXCOP00000025404.1"/>
        <property type="gene ID" value="ENSXCOG00000018975.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153601"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000112">
        <property type="entry name" value="Netrin G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000005">
        <property type="entry name" value="Netrin G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:001161">
        <property type="entry name" value="Netrin-G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF28">
        <property type="entry name" value="NETRIN-G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017404275" evidence="7">
        <location>
            <begin position="20"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin N-terminal" evidence="9">
        <location>
            <begin position="46"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="390"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="415"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="469" mass="53465" checksum="AEB8B6817A9DF7C1" modified="2018-12-05" version="1">MFWPILLTLQAVCTSVTHAMQHYPAAWGHYDVCKSQIYTEEGLAWDYMACQPEATDMTKYLRVALDPPNITCGDPPETYCALENPYMCNNECDAATEELAHPPELMFDFEGRNPTTFWQSTSWKKYPKPLQVNITLSWNKTIELTDDIVITFESGRPEQMVLEKSLDYGRTWTPYQFYATDCLDAFTMEPKTVNDLSQRTLLDIICTEEYSRGYVWKNDKTVRFEIKDRFALFAGPRLHNMASLYGQLDTTKNLRDFFTITDLRIRLLKPATGATMVDENNLSRYFYAISDIKVQGRCKCNLHANSCVFDKGKLGCECEHNTTGPDCSRCKRHYHGRAWSVGSYLPIPKGTANICKQEFDQTIKTPPKVNIVCPLFLNTEVLFCCWASLSARVCDNAMLRCQNGGTCHHHQRCHCAPGFTGVLCERARCQGPGECDDQLSGRAALHHHPIKHRLFFPLVMILLSVVSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLX5</accession>
    <name>A0A3B5MLX5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLX5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025716.1">
        <property type="protein sequence ID" value="ENSXCOP00000025409.1"/>
        <property type="gene ID" value="ENSXCOG00000018980.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000181026"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000286">
        <property type="entry name" value="Zinc finger protein 770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF58">
        <property type="entry name" value="ZINC FINGER PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="164"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="31"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="219" mass="23457" checksum="0601D526F17F12A6" modified="2018-12-05" version="1">MGRFDFTGSSSGGAAGVIGVTPAPPPPPLHPGLSVPQPSPGPSSSSPSPSTSASASNNPSGSTAVPLVGQSDPRSLHQQFSCMLAPEPEPLYHCGECGKTFTHLSSLRRHLRSHGLTPESQSRKSDCNSPSPERIFCCGECGKRFKKRGHLIQHSVTHSENRPFVCSILCGKMFREPFHLTKHLTVHSAPGKNACMHGETPYMLHKVGIQIQDLHPDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MM71</accession>
    <name>A0A3B5MM71_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024883"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025184.1">
        <property type="protein sequence ID" value="ENSXCOP00000024883.1"/>
        <property type="gene ID" value="ENSXCOG00000018573.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155375"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056850">
        <property type="entry name" value="ARM_UBP34_24_USP9X_Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021905">
        <property type="entry name" value="DUF3517"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055176">
        <property type="entry name" value="UBP24/USP9X/USP9Y_UBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF925">
        <property type="entry name" value="UBIQUITINYL HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25010">
        <property type="entry name" value="ARM_UBP24_USP9X-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12030">
        <property type="entry name" value="DUF3517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22900">
        <property type="entry name" value="UCH_UBL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="10">
        <location>
            <begin position="1551"/>
            <end position="1865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="965"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1586"/>
            <end position="1606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="2385"/>
            <end position="2425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="32"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="972"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1595"/>
            <end position="1606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="2385"/>
            <end position="2399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="2413"/>
            <end position="2425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="2434" mass="276850" checksum="F39949618C897A79" modified="2018-12-05" version="1">MTATTRGSPVGGNDSQGQGQAPDAQSQPPLPQNQTSSPNSSNENSPVSPPDEQGQGEGPPQLEEEEPAFPHTDLAKLDDMINRPRWVVPVLPKGELEVLLEAAIDLSKKGLDVKCEACQRFFRDGLTISFTKILTDEAVSGWKFEIHRCIINNTHRLVELCVAKLSQDWFPLLELLAMALNPHCKFHIYNGTRPSETVPAGAQLADDELYARPPDPRSPKGWLVDLINKFGTLNGFQILHDRFMSGQALNVQIIAALIKPFGQCYEFLTLHTVKKYFLPVIEMVPQFLENLTDEELKKEAKNEAKNDALSMIIKSLKNLASRVPGQEETVKNLEIFRLKMILRLLQISSFNGKMNALNEVNKVISSVSYYTHRHNPEEEEWLTAERMAEWIQQNHILSIVLRDSLHQPQYVEKLEKILRFVIKEKALTMQDLDNIWAAQAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCFKASWTNASKKQREKLLELIRRLAEDDKDGVMAHKVLNLLWNLAHSDDVPVDIMDQALSAHIKILDYSCSQDRDTQKIQWIDRFIEELRTNDKWVIPALKQIREICSLFGEAPQNLSQTQRSPHVFYRHDLINQLQHNHALVTLVAENLSAYMETMRQLSKAEFDPQTVRPGSRYSHVQEVQERLNFLRFLLKDGQLWLCAPQAKQIWKCLAENAVFLCDREACFKWYSKLMGDEPDLDPDINKDFFENNVLQLDPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMDDLELIGLDYLWRVVIQGSDDIANRAIDLLKEIYTNLGPKLQVNQVEIHEDFIQSCFDRLKASYDTLCVLDGDKDSINCARQEAIRMVRVLTVLKEYINECDSDYHEERTILPMSRAFRGKHITLIVRFPNQGRQVDDLDIWSHTNDTIGSVRRGILNRIKANAAHTKIELFIGGEIVDPADDRKLIGQLNLKDKTLITAKLTQVSANMPSSPDSSSDSSTGSPGNHGNHYSDGPNPEVESCLPGVIMSLHPRYISFLWQVADLGCNLNMPQLRDGARVLMKLMPPDNTTVENLRAVCLDHAKLGENSLSPSLDSRFFGPSPSQVLYLIEVVYALLMPASATLGEDASDFQYNFLKSGGLPLVLSMLTRNNFLPSADMETRRGAYLNALKIAKLLLTAVGFGHVKAVAEACQPNADGNIPVSPINQATHDQALVLQSALQNIPNPTSECMLRNVAIRLAQQISDEASKYIPDICVIRAVQKIVWASGCGTVQLVFSNNDEISKIYEKTNAAKEPDGEDEQVCCEALEVMTLCFALMPTALDTLSKEKAWQTFIIDLLLHCHSKSVRQMAQEQFFLMATRCCMGHRPLLFFITLLFTVLGSTAKERAKHAGDYFTLLRHLLNYAYNSNINLPNAEVLLNNEIDWLKRIRDEVKRTGETGVEETILEGHLGVTKELLAFQTPEKKYYIGCEKGGANLIKELIDDFIFPASNVYLQYMKSGEFPTEQAIPVCSTPASINAGFELLVALAVGCVRNLKQIVDTLTDMYYLGCETLTEWEYLPPVGPRPNKGFVGLKNAGATCYMNSVIQQLYMIPPIRNGILAIEGTGTDVDDDMSGDEKQENESNVDPRDEVFSYHHQFDDKPSSKSEDRKEYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFREFFNPVSRCPSEISKSLLIFQVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPRELDMEPYTVAGVAKLEGDDVNPENQVIQQNEPSEPTPPGSSKYRLVGVLVHSGQASGGHYYSYIIQRNGGDGEKNRWYKFDDGDVTECKMDDEEEMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERMDSLDKDSELVKYISELNLSSTKPHQVKMPSVIECSVRKQNVQFMHNRMQYSLEYFQFIKKLLTCNSVYLNPPPGQDHLLPEAEEIAMISAQLAARFLFSTGFHTKKLVRGPASDWYDALCILLRHSKNVRYWFAHNVLFAYPNRFSEYLLECPSAEVRGAFSKLIVFIAHFSLQDGPCPSPTASPGSSTQGCDNLSLSDHLLRAVLNLLRREVSEHGRHLQQYFNLFVMYANLGLAEKTQLLKLNVPATFMLVALDEGPGPPIKYQYAELGKLYTVVSQLVRCCDVSSRMQSSINGNPPLPNPYGDTNLTAPVMPVQQLVAEILFVRTSYVKKIIEDCSNSEETVKLLRFCCWENPQFSSTVLSELLWQVAYSYTYELRPYLDLLLQILLIEDSWQTHRIHNVLKGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSNCSVAYQILQSNGDLKRKWTWAVEWLGDELERRPYTGNPQYTYNNWSPPVQSNETSNGYFLERSHSARMTLAKACELCPEEEPDEQEAPDDQDSSPPEDTSLYPHSPGTTQFQQVGYDRTNSPIVLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPG1</accession>
    <name>A0A3B5MPG1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MPG1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026299"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026617.1">
        <property type="protein sequence ID" value="ENSXCOP00000026299.1"/>
        <property type="gene ID" value="ENSXCOG00000019639.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160560"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14241">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14241:SF32">
        <property type="entry name" value="VWFA DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="29715" checksum="CDEF4659593C7A32" modified="2025-10-08" version="2">MFQVWDVLTPKLFLHVNRETDLQFINDYKPKGGHRQFRILLHGPVGAGKSSFINSVFSVLEGKICHKATACNIIQTSFTKKYMTYKLKKGDQKNVYPFVLNDMTGLSNINWKHRRVYEKDMKMAMKGHIRDEYKFHPQSALSETDPFFNRNPTVNDKAHILVTVFDANTINQLDESVAETIQEIRDEATELGIPHVAILSKIDEACPEIREDIKNVCQSRALQKKPMDGGFILNVCLFLVNSTKPTALKKENRVTCILW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQ35</accession>
    <name>A0A3B5MQ35_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Lysosomal acid phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Lysosome membrane</location>
            <topology evidence="11">Single-pass membrane protein</topology>
            <orientation evidence="11">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQ35"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023291"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023570.1">
        <property type="protein sequence ID" value="ENSXCOP00000023291.1"/>
        <property type="gene ID" value="ENSXCOG00000017389.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158446"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043202">
        <property type="term" value="C:lysosomal lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007040">
        <property type="term" value="P:lysosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050645">
        <property type="entry name" value="Histidine_acid_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567">
        <property type="entry name" value="ACID PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567:SF180">
        <property type="entry name" value="LYSOSOMAL ACID PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="366" mass="41838" checksum="CAC24589D07FD6DB" modified="2018-12-05" version="1">LQLYRHGDRSPVRAFPTDPYQENFWWFQMSGLCFLNESYIRHEILVRSTDVDRTLMSAEANLAGLYPPEGQQIFQPSLKWQPIPVHTVPENEEKLLSFPLNDCPRYKQLMNETENTAEFINVTTKYRDFIEMVKNKTGLKVATVESVWSVYDTLFCESRHNMSAPHWVTPDVMEKLRFLKDFGFQVLFGIYKQQEKSRLQGGLLLGEIVKNLTKMALFFLVATQHDTTVVALQASLNVFNGKQAPYASCHIIELYREDNGSASVSMFYRNDTSVPPYPVQLPGCAHVCPLEDFVRITKPFISDDRDKECQVPSKGRDRQVIIGLALSGCLLLAVIAVLLGVICRHKESNRNRGYHHVINQEAGEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQW9</accession>
    <name>A0A3B5MQW9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Nuclear receptor subfamily 5, group A, member 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR5 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027880498.1">
        <property type="nucleotide sequence ID" value="XM_028024697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQW9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021879"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022146.1">
        <property type="protein sequence ID" value="ENSXCOP00000021879.1"/>
        <property type="gene ID" value="ENSXCOG00000016349.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114149115"/>
    <dbReference type="KEGG" id="xco:114149115"/>
    <dbReference type="CTD" id="405759"/>
    <dbReference type="GeneTree" id="ENSGT00940000153391"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07167">
        <property type="entry name" value="NR_DBD_Lrh-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06944">
        <property type="entry name" value="NR_LBD_Ftz-F1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000006">
        <property type="entry name" value="Nuclear receptor subfamily 5 group A member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000011">
        <property type="entry name" value="Nuclear receptor subfamily 5, group A, member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016355">
        <property type="entry name" value="NR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24086:SF48">
        <property type="entry name" value="FF1D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24086">
        <property type="entry name" value="NUCLEAR RECEPTOR SUBFAMILY 5 GROUP A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002530">
        <property type="entry name" value="Nuc_orph_FTZ-F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="13">
        <location>
            <begin position="10"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="14">
        <location>
            <begin position="218"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="330"/>
            <end position="333"/>
        </location>
        <ligand>
            <name>a phospholipid derivative</name>
            <dbReference type="ChEBI" id="CHEBI:16247"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>a phospholipid derivative</name>
            <dbReference type="ChEBI" id="CHEBI:16247"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>a phospholipid derivative</name>
            <dbReference type="ChEBI" id="CHEBI:16247"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR002530-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="452" mass="51585" checksum="589D64C6447E28C6" modified="2018-12-05" version="1">MNFPCEVDLEEQCPVCGDKVSGYHYGVLTCESCKGFFKRTVQNNKTYVCVENQQCKIDTSKRKRCPFCRFQKCLNVGMRLEAVRADRMRGGRNKFGPMYKHNRALKQQKKALIQARRLRIESNQKEVTFTSNIHSIAILHNTKLNPNQLPSLSLSQPCNSPAVIQHQITSPSNWAMRLENCSNSADLASFAEAIVGLHHTSSRSISTQSPRIPQLEIEFRHCDPHELQLQKKIFSCLQQEQACRHREANTFSSVCVMADQLLCSIVEWARTSIFFKELKVSDQMRLLHSCWSELLVLEIISRQVLYGIDGSLLLITGQQGELSYVASHAGSALVSLNQRGTELVEKLRMLKVDHQEFACIRFLILFAPDVKELEEHHFVESVQQQVEGALLKYTLASSSQSCNRFTCLQLYLSELRSLSILAEDYLYYKHLSGEVPCNNLLNEMLHAKCCWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MR17</accession>
    <name>A0A3B5MR17_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MR17"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023636"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023919.1">
        <property type="protein sequence ID" value="ENSXCOP00000023636.1"/>
        <property type="gene ID" value="ENSXCOG00000017657.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158733"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01927">
        <property type="entry name" value="cyclophilin_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000003">
        <property type="entry name" value="Peptidylprolyl isomerase domain and WD repeat-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000368">
        <property type="entry name" value="Peptidylprolyl isomerase domain and WD repeat-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000119">
        <property type="entry name" value="peptidylprolyl isomerase domain and WD repeat-containing protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF4">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE DOMAIN AND WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0697">Rotamase</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="182"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="329"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="9">
        <location>
            <begin position="543"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="72"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="699" mass="79094" checksum="EC57DFA5F02261D9" modified="2018-12-05" version="1">MSQIKLENLFRIYSSLRACLESILGKAKIYIYVHYNELFGAIKEIDWKPEPTGSTAIKEGPDGSFAEMAATENSVEMKRKADDSQEKERGTDGDGDGEDADWVGPMPSEATKAKKRKVLEFERVYLDNLPSAAMYERSYMHRDVITHLVCSKTDFIITASQDGHVKFWKKKEDEGIEFVKHFRSHLSVIESIAVSAEGALLCSVGDDQAMKVFDVVNFDMINMLKMGFHPGQCEWIYNPGDAICTVACSEKPTGKIFVYDGRGSNEPLHLFDKLHSSPLTQIRLNPKFRVIVSADKAGMLEYWTGLPNEFKFPKHVGWAYKTDTDLYEFAKHKTYPVSLAFSPDGKKMATIASDRKVRIFRFLTGKLMRVFDESLTMFTELQQMRPQLPDMEFGRRMAVERELEKVDAVRLTNIIFDETGHFVLYGTMLGIKVINVETNRCVRILGKQENIRVVQLCLFQGVAKAMEVAPTIEMKASDNPALQNVEPDPTIFCTAFKKNRFYMFTKREPEDTKSADSDRDIFNEKPSKEEVMAATQAEGPKRVSDSAIIHTTMGDIHLKLFPVECPKTVENFCVHSRNGYYNGHIFHRVIKSFMIQTGDPTGTGMGGESIWGGEFEDEFHSTLRHDRPYTLSMANAGAGTNGSQFFVTVVPTPWLDNKHTVFGRCIKGMEVVQRISNAKVNPKTDKPYEDISIINITIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRI0</accession>
    <name>A0A3B5MRI0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MutL C-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRI0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023796"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024080.1">
        <property type="protein sequence ID" value="ENSXCOP00000023796.1"/>
        <property type="gene ID" value="ENSXCOG00000017774.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155381"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032389">
        <property type="term" value="C:MutLalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.100:FF:000001">
        <property type="entry name" value="Mismatch repair endonuclease pms1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1540.20:FF:000019">
        <property type="entry name" value="PMS1 homolog 2, mismatch repair system component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="entry name" value="MutL, C-terminal domain, dimerisation subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.100">
        <property type="entry name" value="MutL, C-terminal domain, regulatory subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042121">
        <property type="entry name" value="MutL_C_regsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF52">
        <property type="entry name" value="MISMATCH REPAIR ENDONUCLEASE PMS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08676">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="DNA mismatch repair protein MutL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MutL C-terminal dimerisation" evidence="1">
        <location>
            <begin position="7"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="191" mass="21983" checksum="A056726C9476FDDB" modified="2018-12-05" version="1">MFKEMEIIGQFNLGFIITKLNSDIFMIDQHATDEKYNFEMLQQHTVLHGQRLIVPQKLHLTAVSENVLLENVEIFQKNGFEFLIDEDAQVMERVKLVSLPTSRNWTFGPADIEELIFMLSDSPGVMCRPSRVRQMFASRACRKSVMIGTALSVGEMKKLVIHMGEIEHPWNCPHGRPTMRHLANLEIISQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRY0</accession>
    <name>A0A3B5MRY0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRY0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022518.1">
        <property type="protein sequence ID" value="ENSXCOP00000022249.1"/>
        <property type="gene ID" value="ENSXCOG00000016617.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244823"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF381">
        <property type="entry name" value="TRACE AMINE ASSOCIATED RECEPTOR 19P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="229" mass="25879" checksum="E76068C87DB36E20" modified="2018-12-05" version="1">MCVSLCFLICNLISASIWNIVLISVDRYIAICYPLDYSRKMSLTRVKYCVCLCWFCASSCSFVYSNDVMVQFGKSKSCTGECKFTISYILGTFDLILNFILPVTTIIVLYLRVFVVAVSQARAMRSHITVASFHSATSGTKKSELKAARTLGVLVVVYLMCYCPYYCYSIVQVNITSTSYSSILFILFYSNSCLNPVIYALFYPWFRKAVKVIVTLQILQPGSREAKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MS43</accession>
    <name>A0A3B5MS43_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 242</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TMEM242</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Scaffold protein that participates in the c-ring assembly of mitochondrial ATP synthase (F(1)F(0) ATP synthase or complex V) by facilitating the membrane insertion and oligomer formation of the subunit c/ATP5MC3. Participates in the incorporation of the c-ring into vestigial complexes. Additionally influences the incorporation of subunits MT-ATP6, MT-ATP8, ATP5MJ, and ATP5MK in the ATP synthase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM242 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MS43"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026316"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026635.1">
        <property type="protein sequence ID" value="ENSXCOP00000026316.1"/>
        <property type="gene ID" value="ENSXCOG00000019650.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008642"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009792">
        <property type="entry name" value="TMEM242"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13141">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13141:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07096">
        <property type="entry name" value="DUF1358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="141" mass="14774" checksum="0425201B4FC1AE51" modified="2018-12-05" version="1">METRRRHVTDSPGFLFAGAVFLTTVASAGMIAGFGSTLALAKKKSPDWFSKGVAATAAAPESGASLALRALGWGSLYAWCGVGLLSVAVWKALGVHSLAEFRQKMELFFPAIPRNPEAAAGSEPLDWDAIFGSKSQGSARD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MTW3</accession>
    <name>A0A3B5MTW3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">RUNX1 partner transcriptional co-repressor 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MTW3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026936"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027267.1">
        <property type="protein sequence ID" value="ENSXCOP00000026936.1"/>
        <property type="gene ID" value="ENSXCOG00000020120.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183176"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.140.2220:FF:000001">
        <property type="entry name" value="CBFA2/RUNX1 translocation partner 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1110:FF:000001">
        <property type="entry name" value="RUNX1 translocation partner 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1110">
        <property type="entry name" value="TAFH/NHR1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013290">
        <property type="entry name" value="CBFA2T1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013289">
        <property type="entry name" value="CBFA2T1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014896">
        <property type="entry name" value="NHR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037249">
        <property type="entry name" value="TAFH/NHR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003894">
        <property type="entry name" value="TAFH_NHR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10379">
        <property type="entry name" value="MTG8 ETO EIGHT TWENTY ONE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10379:SF5">
        <property type="entry name" value="PROTEIN CBFA2T1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08788">
        <property type="entry name" value="NHR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07531">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01875">
        <property type="entry name" value="ETOFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01876">
        <property type="entry name" value="MTG8PROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00549">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158553">
        <property type="entry name" value="TAFH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51119">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TAFH" evidence="13">
        <location>
            <begin position="83"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="12">
        <location>
            <begin position="481"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="203"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="371"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="527"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="420"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="203"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="375"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="527"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="554"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="578"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="589" mass="65135" checksum="09DD811BE0DADC35" modified="2018-12-05" version="1">MPDSPADVKTQSRLTPPTMPPPPSTQGATRNSSYTPTTLTNGSSHSPTALNGAPSPPNGYSNGPSSSSSSSLANQQLPPACGARQLSKLKRFLTTLQQFGNDISPEIGERVRTLVLGLVNSTLTIEEFHSKLQEATNFPLRPFVVPFLKANLPLLQRELLHCARLAKQNPAQYLAQHEQLLLDTSTTSPVDSSELLLDVNENGKRRTPDRTKENGFERDGAIHPDVHPSKRPCTMSPAQRFSPSNGLSYQPNGLPHPGPLPPQHYRLDDMAIAHHYRDTYRHPASNHREIRERARPVGMHAGSRQEEVIDHRLTDREWAEEWKHLDHLLNCIMDMVEKTRRSLTVLRRCQEADREELNYWIRRYSDAEELKKGASSGQSRQQSPAAQENATSEIHRELLHRPVSGYVPEEIWKKAEEAVNEVKRQAMSELQKAVSEAERKAHEMISSERAKMERTVAEAKRQAAEDALSIINQQEDSSESCWNCGRKASETCSGCNTARYCGSFCQHKDWEKHHHVCGQTLQAQQQQQQQQSSQQQGGAPGSETPAPISSSACTPSSGTGSPAATPPAATPRSSTPGIPSSSALDSTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MU06</accession>
    <name>A0A3B5MU06_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Ionotropic glutamate receptor C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Postsynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MU06"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022964"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023241.1">
        <property type="protein sequence ID" value="ENSXCOP00000022964.1"/>
        <property type="gene ID" value="ENSXCOG00000017163.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155610"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015276">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000240">
        <property type="entry name" value="Glutamate receptor ionotropic, kainate 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="19">
        <location>
            <begin position="7"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="16">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="16">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="124"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="265" mass="29755" checksum="684BF7EF9DAFB22A" modified="2018-12-05" version="1">MPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGVVEDGATMIFFKKTKISTYDKMWEFMSSRRHSVMVKNIEEGIHRVLTSDYAFLMESTTIEFVTQRNCNLTQIGGLIDSKAYGVGTPMGSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEESKEASALGVQNIGGIFIVLAAGLVLSVFVAVGEFLYKSKQNAQLEKVKNSCDIFSEEFKPMKAEFLTVSTYAMYGSLEANTSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUP3</accession>
    <name>A0A3B5MUP3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA transcription, translation and transport factor protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RTRAF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MUP3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023209"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023487.1">
        <property type="protein sequence ID" value="ENSXCOP00000023209.1"/>
        <property type="gene ID" value="ENSXCOG00000017339.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005163"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019265">
        <property type="entry name" value="RTRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15924">
        <property type="entry name" value="CLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10036">
        <property type="entry name" value="RLL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="239" mass="27698" checksum="6EDDD784A7A7773F" modified="2018-12-05" version="1">MFRRKLTALDYHDPNGFDCSDETQFRNCVVWLEDQKIRHYKIEDRGNLRNIPSSDWPKAYEKYLQDLNCPFGVQEKKEALDWLLGLAVRYEYGDNVEKYKNCQPLDTSANSDKAVDPLVNLDSNSPDFKAGVTALANILKIQRHDDYLVMLKAIRILIQERLSPEAITKANQNREVMRLNLSFFWFQTHATLNEAAQILRLLHIEELRELQTKINEAIVAVQAIIADPKTDHRLGKVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MV54</accession>
    <name>A0A3B5MV54_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MV54"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027683"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028018.1">
        <property type="protein sequence ID" value="ENSXCOP00000027683.1"/>
        <property type="gene ID" value="ENSXCOG00000020641.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183131"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042984">
        <property type="term" value="P:regulation of amyloid precursor protein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039862">
        <property type="entry name" value="NECAB1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12178">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12178:SF2">
        <property type="entry name" value="N-TERMINAL EF-HAND CALCIUM-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="58"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="182" mass="20372" checksum="C07A3B96B398FA3C" modified="2018-12-05" version="1">MNSVLCKSCSSLGLVVFLYLYDLLSPDNLKAILFSVSPADDGKLSLDEFQAFFSDGTLNEEELEKLFHTIDSDNTSNVDTKELCDYFAKHMGDYEGVLASLETLNLSILRAMDFTKRVGMRNDCSRPLHCTKEFSSSVKEWTSTHGWVGNENGPVDHSAKIYVTTVLAKLRCLKKNKPDKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVV8</accession>
    <name>A0A3B5MVV8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Parathyroid hormone</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Parathyroid hormone elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Acts by binding to its receptor, PTH1R, activating G protein-coupled receptor signaling. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with PTH1R (via N-terminal extracellular domain).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the parathyroid hormone family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVV8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027741"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028076.1">
        <property type="protein sequence ID" value="ENSXCOP00000027741.1"/>
        <property type="gene ID" value="ENSXCOG00000020683.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000170472"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003625">
        <property type="entry name" value="PTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001415">
        <property type="entry name" value="PTH/PTH-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10541">
        <property type="entry name" value="PARATHYROID HORMONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10541:SF2">
        <property type="entry name" value="PARATHYROID HORMONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01279">
        <property type="entry name" value="Parathyroid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00087">
        <property type="entry name" value="PTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="7" id="KW-0372">Hormone</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017200869" description="Parathyroid hormone" evidence="10">
        <location>
            <begin position="17"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00093407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="96" mass="11381" checksum="42E951724F1C932E" modified="2018-12-05" version="1">MKLLLFLSLCLRNISLKRTVSEVQLMHNLRHHQQVQERREWLQTRIQDIHNAAGRASSGDTEGWKRRLHPEELTELTPEEIKEALDFLENLLKSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MW16</accession>
    <name>A0A3B5MW16_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Purine nucleoside phosphorylase</fullName>
            <ecNumber evidence="3 13">2.4.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Inosine-guanosine phosphorylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Preferentially acts on 6-oxopurine nucleosides including inosine and guanosine.</text>
    </comment>
    <comment type="function">
        <text evidence="13">The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>2'-deoxyguanosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:27738"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17172"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 13">
            <text>2'-deoxyinosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27750"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:28997"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 13">
            <text>guanosine + phosphate = alpha-D-ribose 1-phosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:13233"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:16750"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 13">
            <text>inosine + phosphate = alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27646"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the PNP/MTAP phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.1" evidence="3 13"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MW16"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027821"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028156.1">
        <property type="protein sequence ID" value="ENSXCOP00000027821.1"/>
        <property type="gene ID" value="ENSXCOG00000020745.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182991"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047975">
        <property type="term" value="F:guanosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004731">
        <property type="term" value="F:purine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09009">
        <property type="entry name" value="PNP-EcPNPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1580:FF:000004">
        <property type="entry name" value="Purine nucleoside phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011270">
        <property type="entry name" value="Pur_Nuc_Pase_Ino/Guo-sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011268">
        <property type="entry name" value="Purine_phosphorylase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01700">
        <property type="entry name" value="PNPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01697">
        <property type="entry name" value="PNPH-PUNA-XAPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006054">
        <property type="entry name" value="PRK08202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904">
        <property type="entry name" value="METHYLTHIOADENOSINE/PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904:SF24">
        <property type="entry name" value="PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000477">
        <property type="entry name" value="PurNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017219435" description="Purine nucleoside phosphorylase" evidence="14">
        <location>
            <begin position="21"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="15">
        <location>
            <begin position="5"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="266" mass="29083" checksum="CA6CE584C1AF3E00" modified="2018-12-05" version="1">RHRPKVAVICGSGLGLLAEAATDKQTFRYQDIPNFPVSTVPGHEGCLVFGTIEGTPCVFMKGHFHLYEGYSLCQVTFPVRIFKLLGVDSVLVTNASGGICPEFKVGDIMVIRDHINLPGFAGQHPLCGPNDERFGTRFPCMSDAYSKELRTLALDVASDLGCSVRTGVYCMVSGPNFETIAEARMLLTLGCDCVGMSMVPEVTVAKHCGLRVLGLTLVTNKVSLDYSREEKVNHDEVLEICKMRAELLQKLVSTLIGRCQQNINTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWI4</accession>
    <name>A0A3B5MWI4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cationic amino acid transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-arginine(in) = L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:32143"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-lysine(in) = L-lysine(out)</text>
            <dbReference type="Rhea" id="RHEA:70935"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-ornithine(in) = L-ornithine(out)</text>
            <dbReference type="Rhea" id="RHEA:71199"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWI4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024044"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024330.1">
        <property type="protein sequence ID" value="ENSXCOP00000024044.1"/>
        <property type="gene ID" value="ENSXCOG00000017957.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155349"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015189">
        <property type="term" value="F:L-lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097638">
        <property type="term" value="P:L-arginine import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000024">
        <property type="entry name" value="High affinity cationic amino acid transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000009">
        <property type="entry name" value="Low affinity cationic amino acid transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004755">
        <property type="entry name" value="Cat_AA_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00906">
        <property type="entry name" value="2A0303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF88">
        <property type="entry name" value="HIGH AFFINITY CATIONIC AMINO ACID TRANSPORTER 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="97"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="293"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="342"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="389"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="495"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="522"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="556"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="581"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="14">
        <location>
            <begin position="554"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="652" mass="70665" checksum="0D95704D44F5986E" modified="2018-12-05" version="1">MVLEKLLGFGKQLLRVKVVNSNSEDSRLSRCLNTFDLVALGVGSTLGAGVYVLAGAVARDNSGPAIILSFLIAASASVLAGLCYAEFGARVPKTGSAYLYSYVTVGELWAFITGWNLILSYIIGTSSVARAWSATFDELVGKRIEEFCREHMSMNAPGVLAEYPDMFAVLIIFTLTGLLAFGVKESAMVNKVFTCVNVLVLLFMVISGLVKGTLKNWQVVPEEILHVNHTNSSSQNLTAILPSKESIGQGGFMPFGFTGVLSGAATCFYAFVGFDCIATTGEEVKNPQRAIPIGIVSSLLICFVAYFGVSAALTLMMPYYLLDSNSPLPVAFKYVGWGGAKYAVAVGSLCALSTSLLGAMFPMPRVLWSMADDGLLFKFMAEISPRTKTPLTATFASGTGAAIMAFLFDLKDLVDLMSIGTLLAYTLVAACVLVLRYQPEIHGPVYQIANTHDDPDSSDGISVPSMGILPGVEERFNFQTLLFPNNPEPSALSGFTVNICTSAIGMLILAFSILAVQGGLTSWNMVALSVIFMVCLVLVFIIWRQPESKTKLSFKVPLLPFIPVISMFVNVYLMMQLDRGTWVRFSIWMAIGLVIYFCYGIRHSSESTSARLSLVTEMNGFNLDHELEAMSIEKEAFLNDGLDVREENDRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWV7</accession>
    <name>A0A3B5MWV7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PiggyBac transposable element-derived protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWV7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024289"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024580.1">
        <property type="protein sequence ID" value="ENSXCOP00000024289.1"/>
        <property type="gene ID" value="ENSXCOG00000018136.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000172289"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd19757">
        <property type="entry name" value="Bbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029526">
        <property type="entry name" value="PGBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46599:SF6">
        <property type="entry name" value="DUAL SPECIFICITY PHOSPHATASE 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46599">
        <property type="entry name" value="PIGGYBAC TRANSPOSABLE ELEMENT-DERIVED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13843">
        <property type="entry name" value="DDE_Tnp_1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PiggyBac transposable element-derived protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="431" mass="49760" checksum="17E355D7AD8A0129" modified="2018-12-05" version="1">MLRSIQEWTTQHARHTEQQDWFMGLPELMAFISVVILREVNKVPSLCDSWSANLGNPRIIATMARCRFQNIMRHLRFDDMLTRRERAETDKFAAISDVWRAFVTNCIASFNPGRHITIDEQLYPSKTRCCFLQYIATKPDKFGIKFWVACDLKSKYICNVFPYLGKDPSRPSGERLSESVVMRLMEPFMDKGRTVTTDNFFTSLSLAQRLLSRKTTILGTVNKRRREIPQSASQTDRTAFTTQVFSTTGATLTVYAPKRKKAVYILSSMHSVVETEDTTKRKPNTVTDYNKTKCGVDVMDQMVREYSVRAGTRRWPVAVFYNMIDMAALNAHVLYQACIGVKERRVDFLVELAKELGNFHVNEKKAHKEKLLREQPSTPSPGKRAKCQVNHRCKTNNATVRCVDCYKYTCGKCTRVIPWLCQVFYHANFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXF5</accession>
    <name>A0A3B5MXF5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Guanine nucleotide-binding protein-like 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXF5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024665"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024963.1">
        <property type="protein sequence ID" value="ENSXCOP00000024665.1"/>
        <property type="gene ID" value="ENSXCOG00000018425.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158320"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04178">
        <property type="entry name" value="Nucleostemin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1580.10:FF:000002">
        <property type="entry name" value="Guanine nucleotide-binding protein-like 3 (nucleolar)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014813">
        <property type="entry name" value="Gnl3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF11">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08701">
        <property type="entry name" value="GN3L_Grn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="8">
        <location>
            <begin position="133"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="9"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="57"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="115"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="467" mass="52121" checksum="3B47EB8050BFA0B5" modified="2018-12-05" version="1">SFSLELRKASKRVSCSKRYKIQKKVREHNKKLKKEAKKKGLKKPVKKDPGVPNSAPFKEDVLREAEQRRLQIEEEKERKRQAKKEERAQKRKNEKEAAVKEAEPSAKKARKDTTENNTVKRSAPNKNSKQHLCSELNKVIDASDVVIEVLDARDPLGCRCPQLEEAVLLGGKRKLLFVLTKIDLVPKENAEKWIECLQREFPVVAFKASTQMRDKTVVRQSKRSHFTALVESKGATCFGSECLTELLTAYAAKTQSEASLKVGVVGFPNVGKSSLINNLKGMMACHVGIAKGTTKTVQEVHMSKTVNLMDSPGIVASPSNPAASMALRSLQVEEGQETVLDAVNTLLKQCDKSQVMLQYTVPDFRNSLEFLTLLAKKRGFLQKGGVPNTVQAANTFLTDWTGAKLRYHCKVPGNYSLPNYVTEEVITEMQNGWDLDKVKAGNMKTLKGINVTAEFTNLDACEQFIVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXS9</accession>
    <name>A0A3B5MXS9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">The B regulatory subunit might modulate substrate selectivity and catalytic activity, and might also direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
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    </dbReference>
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        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B'' SUBUNIT BETA"/>
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        <location>
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        <location>
            <begin position="68"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="531" mass="61370" checksum="1289403FAFE7E35B" modified="2018-12-05" version="1">MPSPQVLQPVLKVKVDELFLNWLSDPGTQSVLKDFLDLIKSGEYNGLSSGNAQDKKSLSFNENNNVASQKNLSEKKPLGTNSSPAPNSTLPSRSSSNARVTGPNGRVLRRSVSTKKAQVRTEEPVTTALSENIPKFYFPQGRPQANLNIDSLISKIEKIFSQFPNERATIEDMGLVAKACECPLYWKMPLFYSAGGDRTGFVSVHKFVAMWRKTLQTCHDDASKFVHLLAKPGCNYLEQDDFIPFLQDVVNSHAGLAFLKEAPDFHSRYITTVIQRIFYNVNRSWTGKITFSELKKSNFLQNVALLEQEEDVNQLTEYFSYEHFYVIYCKFWELDSDHDLYIDQKDLVRHNDQAISQKLIERIFSGTVTRDRRVYKEGRLSYADFVWFLISEEDKKTDTSIEYWFRCMDLDGDGVLSMYELEYFYDDQCQKLEAMAIEPLPFEDCLCQMLDLVKPEVEDKITLRDLKRCKLAHIFFDTFFNIEKYLNHEQRDPFSVIRTDGQEVSDWEKYAAEEYDILVAEEAENNHCNDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZ85</accession>
    <name>A0A3B5MZ85_9TELE</name>
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        <submittedName>
            <fullName evidence="10">Twisted gastrulation BMP signaling modulator 1a</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
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        <scope>IDENTIFICATION</scope>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
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            <location evidence="1">Secreted</location>
        </subcellularLocation>
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        <text evidence="2">Belongs to the twisted gastrulation protein family.</text>
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    <dbReference type="STRING" id="32473.ENSXCOP00000025820"/>
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    </dbReference>
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        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
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    <proteinExistence type="inferred from homology"/>
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        </location>
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            <end position="217"/>
        </location>
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    <feature type="domain" description="Tsg N-terminal" evidence="9">
        <location>
            <begin position="26"/>
            <end position="84"/>
        </location>
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    </evidence>
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            <dbReference type="Pfam" id="PF23782"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="217" mass="23847" checksum="2EFE8760FAE7A78D" modified="2018-12-05" version="1">MRSARLILPATAALLFLLAGLSLTSGCNKALCASDVSKCLIQELCQCRPSDGNCSCCKDCMLCLGNLWEECCDCVGMCNPKNYSDSPATSKSTVEELHRPIPSLFRALTEGDAPINMMVVSFPVAEEFTHHENLVSFLESLNSQPQNVSVPGNSIHTEKMCTVVYFDDCVSIRQCKQYCESMGGSKYRWFHNACCQCIGPECLDYGSKAVKCLNCLF</sequence>
</entry>
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    <accession>A0A3B5N051</accession>
    <name>A0A3B5N051_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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            <taxon>Xiphophorus</taxon>
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    </organism>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSXCOT00000028317.1">
        <property type="protein sequence ID" value="ENSXCOP00000027977.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="124" mass="13520" checksum="BF396EC1887BF3A7" modified="2018-12-05" version="1">MTKSESKRSGVSAGKCASAASAAEQVNNGSFDVGFPEPSPAAKAKVLKQTSKESLYRRNAECEVYDDGTNTFFWLSELHRTGICSPPLLLCGCQPGGKQRALPSSSCRTGWIRRWNQEKILRPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N0D7</accession>
    <name>A0A3B5N0D7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 11">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 11"/>
    <dbReference type="RefSeq" id="XP_027868475.1">
        <property type="nucleotide sequence ID" value="XM_028012674.1"/>
    </dbReference>
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    <dbReference type="STRING" id="32473.ENSXCOP00000026540"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026862.1">
        <property type="protein sequence ID" value="ENSXCOP00000026540.1"/>
        <property type="gene ID" value="ENSXCOG00000019811.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114141826"/>
    <dbReference type="KEGG" id="xco:114141826"/>
    <dbReference type="CTD" id="55033"/>
    <dbReference type="GeneTree" id="ENSGT00940000157858"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000006">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052273">
        <property type="entry name" value="PPIase_FKBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46222:SF1">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46222">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP7/14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017320271" description="peptidylprolyl isomerase" evidence="12">
        <location>
            <begin position="20"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="13">
        <location>
            <begin position="45"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="135"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="179"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="211" mass="24418" checksum="E2B41354EC3E9A1E" modified="2018-12-05" version="1">MMLFTLCSLLPSLFVFVTGGKLPEADVKIEVMHKPFQCHRKSKYGDMLLVHYEGFLESNGTMFHSSRKHGDRNPVWFTLGIREVLKGWDKGLQNMCAGERRKLTVPPSLGYGKEGQGKIPPSSTLIFDIELMEIQNGPRSHDSFRDMDLNDDWKLSRQEVKHFLKKEFEKHGYSPNDTHHEVMVDDIFKNEDEDKDGFISVREFTSIHDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KJ30</accession>
    <name>A0A3B5KJ30_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GDP-fucose protein O-fucosyltransferase 1</fullName>
            <ecNumber evidence="4">2.4.1.221</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Peptide-O-fucosyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-seryl-[protein] + GDP-beta-L-fucose = 3-O-(alpha-L-fucosyl)-L-seryl-[protein] + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63644"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:17914"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:189632"/>
            <dbReference type="EC" id="2.4.1.221"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:63645"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonyl-[protein] + GDP-beta-L-fucose = 3-O-(alpha-L-fucosyl)-L-threonyl-[protein] + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70491"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:17915"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:189631"/>
            <dbReference type="EC" id="2.4.1.221"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:70492"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 65 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.221" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027872587.1">
        <property type="nucleotide sequence ID" value="XM_028016786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KJ30"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000103"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000104.1">
        <property type="protein sequence ID" value="ENSXCOP00000000103.1"/>
        <property type="gene ID" value="ENSXCOG00000000081.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114144217"/>
    <dbReference type="KEGG" id="xco:114144217"/>
    <dbReference type="CTD" id="23509"/>
    <dbReference type="GeneTree" id="ENSGT00390000015634"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046922">
        <property type="term" value="F:peptide-O-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008593">
        <property type="term" value="P:regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11302">
        <property type="entry name" value="O-FucT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11340:FF:000001">
        <property type="entry name" value="GDP-fucose protein O-fucosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11350:FF:000004">
        <property type="entry name" value="GDP-fucose protein O-fucosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019378">
        <property type="entry name" value="GDP-Fuc_O-FucTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039922">
        <property type="entry name" value="POFUT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21420">
        <property type="entry name" value="GDP-FUCOSE PROTEIN O-FUCOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21420:SF3">
        <property type="entry name" value="GDP-FUCOSE PROTEIN O-FUCOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10250">
        <property type="entry name" value="O-FucT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0294">Fucose metabolism</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017369070" description="GDP-fucose protein O-fucosyltransferase 1" evidence="17">
        <location>
            <begin position="28"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="397" mass="44793" checksum="FB608406475B3706" modified="2018-12-05" version="1">MAVRFQAGDVFRGKLGFLFLLLHACLGSSEELKWDENGYVLYCPCMGRFGNQADHFLGSLAFAKMLNRTLAVPPWIVYRHHTPPYTNVHVPYQEFFQLGALSAYHRVVSLEDFMEVLAPRHWPPGQRKAYCFETAAQRSVDKKTCPMKDGNPFGPFWDYVGVEFDQSILFGGISFSAYHQPQWIKKFPASEHPVLALPGAPAQFPVVEEHVGLQRYVVWSENMVKEGEEHIAALLVRPYVGIHLRIGIDWQNACRLLESGDTGPHFMASPQCVGYNRQTAPPLPMSACLPNLKEISRTVQRWVRKTAARSVYIATDSESHSRDIEKLFKGKVKVVSLQPDLAQTDLYILGQADHFIGNCVSSFSAFVKRERDVSGLPSSFFGIETPTNTKKEPKQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KK83</accession>
    <name>A0A3B5KK83_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the IFIT family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KK83"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000593"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000602.1">
        <property type="protein sequence ID" value="ENSXCOP00000000593.1"/>
        <property type="gene ID" value="ENSXCOG00000000514.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182946"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000036">
        <property type="entry name" value="interferon-induced protein with tetratricopeptide repeats 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10271">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN WITH TETRATRICOPEPTIDE REPEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10271:SF14">
        <property type="entry name" value="INTERFERON-INDUCED PROTEIN WITH TETRATRICOPEPTIDE REPEATS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="306" mass="35822" checksum="DB49279656FD07B7" modified="2018-12-05" version="1">MTFISSPQSLQSRLKALESHFTWDLTPSRDKLSRIRDKLEDIGTEEGNIWLGHVYNLLGFIQYQLGSSEDALNLFNRAAETFQRQKNADEGPWLMVNFGNLAWLHHHLGEDEKSEDYLSKVDGLMKKKGDLYPEVLAEKAWTLMKFDKEKKQQALELFQRAIRMQPDTVEWRSSRMIGLLSTFKHSWVEPEPDIWEDLRVAREEDPENLYLAAVDLKQRAKRGERVKEEAQELSEKILLNPVSSHSGIKPLLRIYRQIESYDDAIDVAERALTKDPDSRYLKRCAALVYKWKIVFFQRWSPKPKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KKV8</accession>
    <name>A0A3B5KKV8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein 39A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM39 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KKV8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000793"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000804.1">
        <property type="protein sequence ID" value="ENSXCOP00000000793.1"/>
        <property type="gene ID" value="ENSXCOG00000000671.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018895"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902902">
        <property type="term" value="P:negative regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901097">
        <property type="term" value="P:negative regulation of autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019397">
        <property type="entry name" value="Uncharacterised_TMEM39"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12995">
        <property type="entry name" value="FI21814P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12995:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 39A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10271">
        <property type="entry name" value="Tmp39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="321"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="418"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="449"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="490" mass="56197" checksum="B98D2837A59D2E2F" modified="2018-12-05" version="1">MPGGRRGPSRQQLSRSSLPSLQTLVGGSVSNGTGLRNSNSVGLSAPPISTLITPEPVRHSRIPELPLDSSFLFEFLLFLYLLVALFVQYINIYRTVWWYPYSHPAASTSLNFHLMDYHLAIFITVMLARRLVWTIVSEVNKLGGSLFRYIVLITARLSLLTMCGWVLCWTLVNLCRNHSVLNLLFLGYPFGVYVPLCCFHQEGGKSQAPGSDCEYPSEHQQADLAESQLFRPRDFLFLLRENIREQFSSPPHMPTHTCPQHIHSDTPELIRAEVEELKSDFNRRIKEVLFNSLFSAYYVAFLPLCFVKSTQYYDMRWSCEHLIMVWINAFVMLMSQLLPPSYCDLLHRSAAHLGRWQKLEPGSYSNTPQHVWSESTVWPQGVLVRHSRCLYKAVGSYNVALPSDVSHSRFYFLFHKPLRILNLLIGIESSVVLYQLYSLLRSERWNHTLSLGLILLCNYYVLFKLLRDRIVLGKAYSYPLSANSSGLKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KNF1</accession>
    <name>A0A3B5KNF1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSXCOT00000001673.1">
        <property type="protein sequence ID" value="ENSXCOP00000001648.1"/>
        <property type="gene ID" value="ENSXCOG00000001226.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182774"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000065">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002282">
        <property type="entry name" value="Wu:fb97d07 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000765">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000645">
        <property type="entry name" value="Zinc finger and BTB domain containing 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000512">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001158">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001174">
        <property type="entry name" value="zinc finger protein 527 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF416">
        <property type="entry name" value="FI01424P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="186"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="214"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="242"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="270"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="326"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="354"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="385"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="413"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="441"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="469"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="497"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="525"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="52"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="656" mass="74858" checksum="05DDFA5D459348CB" modified="2018-12-05" version="1">GLAELVALRLVHFIYDQWFWCILKPEQEEPEHQQFKENQLCISQDEEQLVQKQDTDSPLVENRDQEEIDSENQGKTRDDQKQHEICQTTKQQKGTLPGHRLYSCEVCDKIFSGQKGFTIHMRNHMREKTHGCTTCGKRYYSRSGLNLHMRAHTGEKPFTCATCGKSYGSRVSLNFHMRTHTGERRFKCVVCGKDFRQSSALHTHMRTHSDQKPYSCTTCGKRFSNKSVLNSHIIIHTGEKRYSCIACGKRFSQKSQLRYHMMTHTGEKPVSCMTCGKTFTHKGNLSEHIKTHTGEKPFSCSTCGKCFSHKSLLRNHMRRHTGERPFSCTTCGKTYSERSGLAYHVKTHTAERPFSCVTCKATFRKQSFLTNHMKIHTKCELEKPFSCVTCGKGFLRKSHLICHMRSHTGEKPFSCMTCGKSFSVKCNLTFHMKTHTNEKPFSCATCGKGYHNKSSFNYHMRNHTDEKPFPCVTCGKGFVHNSHLILHMRIHTGEKPFTCISCGKSFTQKSNLAIHMRFHTGEKPFTCISCGKSFTQKSHLATHMRIHTGEKPFTFISCGKSFTQKSNLAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXTYHSQQTAWAQHTPGLTHRKLTLGHHPSSFPFMEAQAVESATPNGTTCYTHQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQ91</accession>
    <name>A0A3B5KQ91_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQ91"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000030"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000030.1">
        <property type="protein sequence ID" value="ENSXCOP00000000030.1"/>
        <property type="gene ID" value="ENSXCOG00000000027.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000179415"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017341109" description="Kazal-like domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="2">
        <location>
            <begin position="15"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="67" mass="7137" checksum="7646693DAD926E9C" modified="2018-12-05" version="1">RLTSLTRLIPLTGLALPTTSCPNPQLYRPPESCRPESEPLCASDGHTYPSECAMTATGVQKGISSCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQX9</accession>
    <name>A0A3B5KQX9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="15">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 15">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Membrane</location>
            <topology evidence="2 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQX9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000877"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000887.1">
        <property type="protein sequence ID" value="ENSXCOP00000000877.1"/>
        <property type="gene ID" value="ENSXCOG00000000729.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160101"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000014">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF80">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE IM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 14 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="297"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="366"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="16">
        <location>
            <begin position="183"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="521" mass="58883" checksum="414290A3528FB36D" modified="2018-12-05" version="1">PETIACLNLADIKIWVLTGDKLETAMSIGYSCNMLRDDMDEVFVISGLTQLEVQQQLRNAKENILGLRRLSSTGDVQKGDVFAEDSVFEETIISEYALVINGHSLAHALEPQLEHTLLDLACLCKTVICCRVTPMQKAQVVELVRRHKMAVTLAVGDGANDVSMIKTAHIGVGISGQEGMQAVLASDYSFAQFRYLQRLLLVHGRWSYFRMCNFLAYFFYKNFAFTLVHFWYGFFCGFSAQTVYDQWFITLFNIVYTSLPVLAMGLFDQDVNDQNSLRYPSLYKPGQQNLLFNKRQFFLCTLQGMVTSFLLFFIPYGAFSVMLKDDSSDFSDQQAFAVTIATTLVIVVSVQIGLDTHYWTAVNHLFMWGSLIVYFAILFAMQSDGLFGIFPSNFPFIGTARNCLSEKSVWLVILLTTVVCILPGLVVSFLRIAGLKKQRPQEQNLRRVRRTSSRRSAYAFSHQQGYGELITSGKNMRVSTLSSSRSLERIAQSSSWIENILKRKNEVSAQPAESHKADRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KR44</accession>
    <name>A0A3B5KR44_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KR44"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000106"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000107.1">
        <property type="protein sequence ID" value="ENSXCOP00000000106.1"/>
        <property type="gene ID" value="ENSXCOG00000000084.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183032"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000015">
        <property type="entry name" value="Protein-L-isoaspartate (D-aspartate) O-methyltransferase domain-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF2">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="325" mass="37048" checksum="CC1D487A9BEDF1B4" modified="2018-12-05" version="1">MGGAVSAGEDNDDLIDNLKEAYYIRTDLVERAFRAIDRADYYLDEYRDNAYKDLAWRHGNIHLSAPCIYSEVMEALELHPGLSFLNLGSGTGYLSTMVGLILGPFGVNHGIELHADVIEYAYQKLDSFIKTSDSFDKFEFCEPSFVVGNCLEIPPESRHYDRVYCGAGVQKEHEEYMKNLLKVGGILVMPLEEKLTKIIRTGPSSWETKKIISVSFAPLVLPKHSPKSKPKTAPLRKSHFRFAEIVLLFVNVPRIITHTKTTQHLRLIKSSHIKLGIILEEEEEEEKETGELLRPQPAVNVLRERILGLPLPEPLKMYLLYYREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTN5</accession>
    <name>A0A3B5KTN5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein lin-28 homolog A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Rough endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the lin-28 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTN5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000976"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000988.1">
        <property type="protein sequence ID" value="ENSXCOP00000000976.1"/>
        <property type="gene ID" value="ENSXCOG00000000815.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153295"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005791">
        <property type="term" value="C:rough endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031054">
        <property type="term" value="P:pre-miRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000007">
        <property type="entry name" value="Protein lin-28 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000087">
        <property type="entry name" value="Protein lin-28 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051373">
        <property type="entry name" value="Lin-28_RNA-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054081">
        <property type="entry name" value="Lin-28A-like_Znf-CCHC_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46109">
        <property type="entry name" value="PROTEIN LIN-28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46109:SF2">
        <property type="entry name" value="PROTEIN LIN-28 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21890">
        <property type="entry name" value="Lin-28A-like_zf-CCHC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="13" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CSD" evidence="19">
        <location>
            <begin position="31"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="18">
        <location>
            <begin position="130"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="96"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="167"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="170"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="202" mass="22209" checksum="E35288943BE39C62" modified="2018-12-05" version="1">MGSVSNQEFPGVCKTEGDEGPSTEEDTQSFHGVGVCKWFNVRMGFGFLSMTNREGVPLDEPVDVFVHQSKLHMEGFRSLKEGEAVEFTFKKSSKGLESQRVTGPGGIHCVGSERRPKGKKIQKRRSKGDRCYNCGGLDHHAKECKLPPQPKRCHFCQSIEHMVASCPVKAQQSSPVSQGKPSPLKGDEEEHSQPAPPPETSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KU11</accession>
    <name>A0A3B5KU11_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Plac8 onzin related protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cornifelin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KU11"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001987"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002017.1">
        <property type="protein sequence ID" value="ENSXCOP00000001987.1"/>
        <property type="gene ID" value="ENSXCOG00000001592.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163927"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006461">
        <property type="entry name" value="PLAC_motif_containing"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01571">
        <property type="entry name" value="A_thal_Cys_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15907">
        <property type="entry name" value="DUF614 FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04749">
        <property type="entry name" value="PLAC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="128" mass="14031" checksum="CFD73346A52F4F1A" modified="2018-12-05" version="1">MLTLILPDMAYHPPSSSPGTWSTGLFDCCKDTSGCLFGLCCFPCMQCQTAKEYGWCCLMPLLDICGVVSCILRSNIRDRHNIPGSGCDDCLKIACCYACVWCQMNRELKIRGSNQSGDNVVTAQVGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KUP8</accession>
    <name>A0A3B5KUP8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KUP8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001751.1">
        <property type="protein sequence ID" value="ENSXCOP00000001725.1"/>
        <property type="gene ID" value="ENSXCOG00000001392.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286915"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001524">
        <property type="entry name" value="Si:dkey-126g1.7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017374861" description="B30.2/SPRY domain-containing protein" evidence="7">
        <location>
            <begin position="20"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="9">
        <location>
            <begin position="117"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="8">
        <location>
            <begin position="794"/>
            <end position="995"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="997" mass="112752" checksum="2B6AC78A6C6F1776" modified="2018-12-05" version="1">HLLPILYLVLLTQTHPTNTFSHNLFYADAAVCQRKLKSALTKKFQCVSEGIDKAGNSTPLNNIYTDLYITESGTKKVNEEHEFRHIETVSRKQNGEEATIGLEQLFRGSPGQNNPVRTVITRGVAGIGKTVLTQKFTLDWAENKTNQDIQFIFPFTFRELNALKGKEFSLVELVHHFFPETKEICEFIEFQVIFIFDGLDECRLPLDFKNNDILTDITKPVSVDALLTNLIRGKLLPTARLWITTRPAAASRIPPDYVDMVTEVSGFDDPQKEEYFRKRFRDEELASKIISHIKTSKTLHNMCHIPLFCWIAATVVENVFKTSQTKELPKTMTEMYIHYLLVQTKVKMVKYDGNNETDEHWSSKRKEMMKSLGKLAFEQLQKGNLIFYESDLRECEISLQGASEYSGMFTQIFKVERVLSDVKLFSFVHLSIQEFLAALHVHLTFTESGVNRLKENTLPTLSALLKKPTILSFYHSAVNKTLQSPNGHLDLFLRFLLGLSLETNQKLLHGLLKQTERSSQINQKTAQYIKEKINENLSTEKSINLFHCLNELKDGSLMQEIQQFLSAGTLRTDKLSPAQWSALVCFLLSSEENLDVFNFTRFSVSENILLRLLPLIKVSKKAVLTFCVLSQSSLEALSSVLSTTSLSRLRELDMSNNILQGSAVNKLSEGLRRSFCQLEILRLNLCCLSETSCEALSAVLSSESASLKELDLSNNNLRDSGVELLSTGLESSYCRLETLRLSGCMVTGEGFASLASALNSNSSHLRELDLTFNHSDDAGVKLLSANLKDPQSKLEVLRYGKWLTPGLRKYYCELTVDTNTMNRNVKLSNNNTKASYVEEAQPYPDHPDRFEKNHQVMCSNGLTGRCYWEVELMGGVHTSVSCKGIIRRGEGNDGWFGGNDQSWTLFFDGNSYSVWHNKKGISIPYTTVVTPTSSHRVAVYLDWPAGTLSFYRVSSDTLIHIHTFHTTFTEALYPGFGLWSWVNAPKGSSVQLISLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KVA8</accession>
    <name>A0A3B5KVA8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="4">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="34 35">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 is involved in the regulation of cell adhesion and cell migration, and plays a central role in heart morphogenesis, angiogenesis and blood vessel remodeling and permeability. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. Involved in somitogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KVA8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002457"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002491.1">
        <property type="protein sequence ID" value="ENSXCOP00000002457.1"/>
        <property type="gene ID" value="ENSXCOG00000001960.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155297"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005005">
        <property type="term" value="F:transmembrane-ephrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05065">
        <property type="entry name" value="PTKc_EphR_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09551">
        <property type="entry name" value="SAM_EPH-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000041">
        <property type="entry name" value="ephrin type-A receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1770:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000015">
        <property type="entry name" value="Ephrin type-B receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000004">
        <property type="entry name" value="Ephrin type-B receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000110">
        <property type="entry name" value="Ephrin type-B receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1770">
        <property type="entry name" value="ephrin a2 ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027936">
        <property type="entry name" value="Eph_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042819">
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        <property type="entry name" value="Ephrin_rcpt_lig-bd_dom"/>
    </dbReference>
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        <property type="entry name" value="Ephrin_rcpt_TKs"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="SAM/pointed_sf"/>
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        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
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        <property type="entry name" value="Tyr_kinase_AS"/>
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    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
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        <property type="entry name" value="Tyr_kinase_ephrin_rcpt"/>
    </dbReference>
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        <property type="entry name" value="Tyr_kinase_rcpt_V_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46877">
        <property type="entry name" value="EPH RECEPTOR A5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EPHRIN TYPE-B RECEPTOR 1"/>
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        <property type="entry name" value="EphA2_TM"/>
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        <property type="entry name" value="FNTYPEIII"/>
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        <property type="entry name" value="TYRKINASE"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="15" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
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    <keyword evidence="27" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
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    <keyword evidence="18 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0524">Neurogenesis</keyword>
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    <keyword evidence="20" id="KW-0675">Receptor</keyword>
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    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
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    <keyword evidence="19" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="484"/>
            <end position="506"/>
        </location>
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    <feature type="domain" description="Eph LBD" evidence="33">
        <location>
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            <end position="144"/>
        </location>
    </feature>
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        <location>
            <begin position="265"/>
            <end position="375"/>
        </location>
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        <location>
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            <end position="471"/>
        </location>
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        <location>
            <begin position="562"/>
            <end position="825"/>
        </location>
    </feature>
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        <location>
            <begin position="854"/>
            <end position="918"/>
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    <feature type="active site" description="Proton acceptor" evidence="25">
        <location>
            <position position="687"/>
        </location>
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        <location>
            <begin position="568"/>
            <end position="576"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="26 28">
        <location>
            <position position="594"/>
        </location>
        <ligand>
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        <location>
            <begin position="4"/>
            <end position="126"/>
        </location>
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        <location>
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            <end position="49"/>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KVM9</accession>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Metazoa</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
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        <location>
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            <end position="27"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="86" mass="9371" checksum="5CF29BBAADA4C788" modified="2018-12-05" version="1">MGQHESKSSVEARVQVLLLGLDNAGKSTLLYKMKHNVGVSTVPTIGFNVEMLDARKNRKNISVTVWDVDSSNLFAPTANLAVILCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KVV5</accession>
    <name>A0A3B5KVV5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ras-associating domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KVV5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002697.1">
        <property type="protein sequence ID" value="ENSXCOP00000002662.1"/>
        <property type="gene ID" value="ENSXCOG00000002124.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182839"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033593">
        <property type="entry name" value="N-RASSF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048945">
        <property type="entry name" value="RASSF8/10_RA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15286:SF11">
        <property type="entry name" value="RAS ASSOCIATION DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15286">
        <property type="entry name" value="RAS-ASSOCIATING DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21712">
        <property type="entry name" value="RASSF8-10_RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-associating" evidence="2">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="330" mass="37300" checksum="E35442E6F8112691" modified="2018-12-05" version="1">VIIEMELKVWVDGVVRVVCGLSLNTSCQEVVISLAQAIGQTGRYVLIRKLRGLERPLVADDCPMQLLAQMGQLAAEVQFVLRKTGPSLSEGPHRPSKESHRPLLVPSEPEPQHKGPQKALSFSLGPSTFPRRNRPTLEKDTELFEQKVTNSVVPDPIPDLTDDLQELEYRRKQYEVELMYGEDWEEELKVELEREQGRQSINQSINQSINQSINQSINQSINQSINQSINQSINQSSLFVEHISAMTQVKILEQIDGLNKELRQCDLQQFIQQAGGNPYADQGAPLLVSEVQLSKTRVRNASLSYSSIVSPRMLEAEKFNPNRCLFPRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KWA6</accession>
    <name>A0A3B5KWA6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Chloride channel K</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KWA6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002817"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002854.1">
        <property type="protein sequence ID" value="ENSXCOP00000002817.1"/>
        <property type="gene ID" value="ENSXCOG00000002235.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164106"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000012">
        <property type="entry name" value="Chloride channel K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050970">
        <property type="entry name" value="Cl_channel_volt-gated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720:SF3">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC-KB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0129">CBS domain</keyword>
    <keyword evidence="10" id="KW-0868">Chloride</keyword>
    <keyword evidence="9" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="166"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="293"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="683" mass="74705" checksum="7FA68558B0811E1E" modified="2018-12-05" version="1">MKSFDKSLEIETEEEDNVQKHENNSEASHNITPLLRDSWKPYKGTRANVKEQLLKLRDCLESVCRIQWYGFAILGILTAVLSFLMDFSVAKLLRAHLWLYAKLEGHVLLQFLCWTLYPACISAFASTFSHNICPFSTGSGIPEVRTMLSGIELPHYLSLTHMFTKFLGLICTLAAGSTVFLGKVGPFVHLSTMLGAYLDRLCRLMEGKKKEAGTEMMVVAAAVGVASCFGAPISGVLFSVEVMSSHFAVRHYCPCFFSAACGALTFHLLSMWSSDGETLQALFKTNFPTASPFFPLETLLFALLGLLCGAVSCCYLFCHRRILKFTKTNRVFSTMLTTEKGLYSGLVVFFLASLTFPYSAGQYLASKCTTKQLLTSLLDSRQWSSQSHNASVQLELGTLDWGSSGAPIFLPLAFFLLMKMWMLVLACTLPLPAGYFMPVFVYGATLGRLFGEGVAYITTGRDILGQRWTSVNPGGYALAGAAAYSGAVTHTLSPALLAMELTGQFTHAVPALLSTLLANALARSRNLPSFYDGLSISKKLPHLPSLKKACPQLPSTPLGQVMGVNSVQLQKAAGPAEVQSAVSNSNEARIPVVDTHGTQRAESYFTNWMIFAFAQCIEGRLDEVCSIHPNSVMLSPHNTVQEAYNMLSVAPAQTIFVAERGKLVGQITWSEMKLILEGLAKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYK4</accession>
    <name>A0A3B5KYK4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KYK4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002711"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002747.1">
        <property type="protein sequence ID" value="ENSXCOP00000002711.1"/>
        <property type="gene ID" value="ENSXCOG00000002155.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000178325"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="71" mass="7820" checksum="140938A705804CA1" modified="2018-12-05" version="1">MASDRGVPGSGVFGDLPPSYTRSQPPAHPDLQRRPSYCHAAFALKQISKVMHFTRSLATHTHALLYVSQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZC0</accession>
    <name>A0A3B5KZC0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZC0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005495.1">
        <property type="protein sequence ID" value="ENSXCOP00000005433.1"/>
        <property type="gene ID" value="ENSXCOG00000004243.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168279"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029377">
        <property type="entry name" value="TMEM220"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34262">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34262:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15071">
        <property type="entry name" value="TMEM220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="166" mass="18871" checksum="B4203409AC4D7FE9" modified="2018-12-05" version="1">FICFNLIQFNLLKLISNFLMSVFFTLASYVQINDPDAGLWMVGYAVPAVLCGFIGFRPHVTETLPWRRVADLHLMISSAAIFMLGWKLYAERATQIFQQEEGREFSGLTLTAAWLLLCRRSGRAPVGKLRVSTAVAITVFPIVAWLYYHINKELRSDWPAHCKTAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZW6</accession>
    <name>A0A3B5KZW6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZW6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003171"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003208.1">
        <property type="protein sequence ID" value="ENSXCOP00000003171.1"/>
        <property type="gene ID" value="ENSXCOG00000002101.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158078"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050748">
        <property type="entry name" value="Glycosyltrans_8_dom-fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13778">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 8 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13778:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 8 DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="241" mass="27624" checksum="80C6FA2798C99C35" modified="2018-12-05" version="1">ITFDQSLAFFGGGFYLSLRQIVTTIAMEKFWSTFLFLSIMTKCPSPEAARPPLTITPPLPCVTVSLLLFCKTDDGVYVDSLLFSELVPQTTYMGFLDYRKQEVKDLAINPNHCSFNPGVFVAEIKEWKKQKITKQLERWMEKNFRQNIYSSAMAGGVATPPMLIVFHDKYTALDPLWHVRHLGWSPDARYAESFLQGAHLLHWNGPFKPWNDPAVHSDLWERWFIPDPSGRFTLTRPGSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L0C6</accession>
    <name>A0A3B5L0C6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription cofactor HES-6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Hairy and enhancer of split 6</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Does not bind DNA itself but suppresses both HES1-mediated N box-dependent transcriptional repression and binding of HES1 to E box sequences. Also suppresses HES1-mediated inhibition of the heterodimer formed by ASCL1/MASH1 and TCF3/E47, allowing ASCL1 and TCF3 to up-regulate transcription in its presence. Promotes cell differentiation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family. Interacts with HES1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L0C6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005849.1">
        <property type="protein sequence ID" value="ENSXCOP00000005783.1"/>
        <property type="gene ID" value="ENSXCOG00000004490.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161398"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000081">
        <property type="entry name" value="transcription cofactor HES-6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00511">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="13">
        <location>
            <begin position="27"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="14">
        <location>
            <begin position="98"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="158"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00081410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="222" mass="24336" checksum="F0F7008882E26AB8" modified="2018-12-05" version="1">MLTAALTPPVFFSVSSRPNVGPGGPAEIQHRKPLVEKKRRARINESLQELRTLLADIDLHSKMENAEVLEMTVKKVEDVLKNRSQEADALSREASERFAAGYIQCMHEVHMFVSNCPGIDATVAAELLNHLLECMPLNEDHLQDVLMDLITDTPGTNSSTWLGAAEGPCPTPALSNSSNDELCSDLDETDSEHNQSSTEGAESGEALSVPTVTNPQCMWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2D2</accession>
    <name>A0A3B5L2D2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA-binding region-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2D2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006473"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006553.1">
        <property type="protein sequence ID" value="ENSXCOP00000006473.1"/>
        <property type="gene ID" value="ENSXCOG00000005013.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063786"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097157">
        <property type="term" value="F:pre-mRNA intronic binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030626">
        <property type="term" value="F:U12 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048565">
        <property type="term" value="P:digestive tract development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001889">
        <property type="term" value="P:liver development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031016">
        <property type="term" value="P:pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd12238">
        <property type="entry name" value="RRM1_RBM40_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12239">
        <property type="entry name" value="RRM2_RBM40_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000948">
        <property type="entry name" value="RNA-binding region (RNP1, RRM) containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000207">
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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        <property type="entry name" value="RBD_domain_sf"/>
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        <property type="entry name" value="RBM40_RRM1"/>
    </dbReference>
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        <property type="entry name" value="RBM41/RNPC3"/>
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    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
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        <property type="entry name" value="RNA-BINDING REGION-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16105:SF0">
        <property type="entry name" value="RNA-BINDING REGION-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="14"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="409"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="208"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="261"/>
            <end position="313"/>
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    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="326"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="490"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="208"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
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        <location>
            <begin position="263"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="336"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="509" mass="57134" checksum="38E2D3C65F4178DD" modified="2018-12-05" version="1">MEKNEDSSKSNGSRTLLVRHLPAELNTDEKEDLLTYFGAESVRVFSNRGRLKHAAFATFKSENMAAKALSRLHQLQILDQTLIVEFAQGQDNLTVLKDPPVSDGFGFNLFSFLHSHVKEKQKTQEFKQPKIPIIETGVSPNLGMRFHSNPTLKYLYPPPSNGVLTNITHALIAVPKFYVQVLHLMNKMNLPCPFGPVTARPPMLLPPAPPFPMPPPPPTDYPPLPEEEMERSSEEESEYESGDEEDKERIARLMGLVNQACKRPLRPKTASKRKKPKIKDLLYAPKPDSQSAPVLQPSDVFESPNPAGPRKIEFHISVPEAAAAVEGSGLARADSAPSSSSQEVTVTEGFGKLYPSTQTNQQQDERSDDEEDLVSDVISRKELEKGRLSRDEIKRMSVFKNYEAGEPTCRLYVKNIAKQVEEKDLRYIYGRYIKPSSEAERNMFDVVLMKEGRMKGQAFIGLPNERSAEKALRETNGFVLHDKPLVVQFARSARPKPDSTNSKKGPKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L422</accession>
    <name>A0A3B5L422_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Collagen IV NC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
            <location evidence="2">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L422"/>
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    <dbReference type="Ensembl" id="ENSXCOT00000002548.1">
        <property type="protein sequence ID" value="ENSXCOP00000002514.1"/>
        <property type="gene ID" value="ENSXCOG00000002004.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164076"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030020">
        <property type="term" value="F:extracellular matrix structural constituent conferring tensile strength"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.240.10:FF:000001">
        <property type="entry name" value="Collagen IV alpha 1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.240.10">
        <property type="entry name" value="Collagen IV, non-collagenous"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001442">
        <property type="entry name" value="Collagen_IV_NC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036954">
        <property type="entry name" value="Collagen_IV_NC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1019">
        <property type="entry name" value="COLLAGEN ALPHA-5(IV) CHAIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01413">
        <property type="entry name" value="C4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00111">
        <property type="entry name" value="C4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="NC1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Collagen IV NC1" evidence="10">
        <location>
            <begin position="300"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="229"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
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        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
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            <end position="98"/>
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            <end position="155"/>
        </location>
    </feature>
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        <location>
            <begin position="280"/>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="523" mass="53821" checksum="F2553CE2206571D7" modified="2018-12-05" version="1">PGPKGHKGDPGDPGGIHMGPPGLNGDPGDQGPRGNRGKPGRFGPIGLPGLPGQLGQKGEPSSNVSPGLKGDKGEPGLPGRPGPNGSPGSLGLPGSKGTPGPPGELGDSGHGHPGRPGPPGPPGENGAPGPAGDPGIPGLPGSPGIIGKSGPPGEKGNCRLKGVKGDPGIPGRPGPDGVPGTTGSKGAAGEPSYVYGPPGPPGQKALMWFFSAVFVLFFSFFNSGPPGDIGPPGLFGRGSPGPEGDRGIPGLPGRPGESGLPGREGTCIPGPKGDGGFPGNPGNQGPRGLPGRAATHYTDGFLIARHSQSIKVPDCPNGSSLIYSGYSLLFINGNERAHGQDLGTMGSCLPRFSTMPFLFCDTENTCRYASRNDYSYWLSTDKLMPANMVAVTADKVAPYISRCSVCETTSKIIAFHSQNTLIPECPRGWETLWTGYSFIMQTGAGAEGSSQPLMSPGSCMENFRRVPFIECHGRGTCNYYPDSYSYWLASLDRNNIFSKPVPRTVKGFSLERVISRCRVCRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6J0</accession>
    <name>A0A3B5L6J0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6J0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007928"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008026.1">
        <property type="protein sequence ID" value="ENSXCOP00000007928.1"/>
        <property type="gene ID" value="ENSXCOG00000005656.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163009"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000024">
        <property type="entry name" value="Serine protease 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253:SF144">
        <property type="entry name" value="CHYMOTRYPSIN-LIKE PROTEASE CTRL-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="63"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="360" mass="38970" checksum="343B293667CEB014" modified="2018-12-05" version="1">MLICSVTTPGPNRRARLGRPRLNFFRNFPVLYFSSVTVNAAHTAACTPTNEVSKRAENSRHWSSWPWQVSLHLSSHFCGGSLVNDQWVLTAAHCVGGLAGIPSLVTVYLGRQSQEGSNPNEVVRKVVQITSHPNYSFPNNDIALLKMSEPVNFTSYISPVCLGASSSSFYSGDPSWVTGWGNIGFNDPLPSPGNLLEVEADIVGNGQCSCDYSELGDLINDNMICAGFREGGKGPCFGDSGGPLVTKQGDRWIQAGIVSFTVDCAKSYFPSGYARVSQYESWINSVINTNQPGFITFTSNRTNSDLNVSCTTSNLTTHPPTTTTEAQRECLDLIDFVIKQKNLPLLGLPPYCGGGVSVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7V2</accession>
    <name>A0A3B5L7V2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Orofacial cleft 1 candidate gene 1 protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L7V2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006990.1">
        <property type="protein sequence ID" value="ENSXCOP00000006902.1"/>
        <property type="gene ID" value="ENSXCOG00000005329.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163681"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031390">
        <property type="entry name" value="OFCC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33862">
        <property type="entry name" value="OROFACIAL CLEFT 1 CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33862:SF3">
        <property type="entry name" value="OROFACIAL CLEFT 1 CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="416"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="474"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="563"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="659"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="781" mass="87306" checksum="33F8A4375B87EE67" modified="2018-12-05" version="1">YYDPFLAEFVLCPFKIYMLHDSQGDQGALPQSIEVCVCCLRAARDKLPRGLYSIRVALQSTLGGSSLHLRSSKEQTWSASTEPVEHRGRFYDNDLHINQSVFITSLPATSEINPSMVLLFQLISLPGHSSHFSSVLAWGVFPICSMSLSLVQGRFKTPLIRGEPSPQLDQFKKIEAIISSDLDHWLCNLYFQVKRIPLGTSGVTELFVAPSRSLSNPKIVSKPEDHHDPTLTQRPTAQSQICPHPLLLQSQQHLHVGPGCRAGESAVVLSQHGSPLHLSADSACSSSSLPGRKVFSGSAVEKTNPCREKAEVGFQYKKKPIKKTNSCGPSMSGRSAPPLQADKQKMQPTEENLSMEEMEGYTFFLQTSPTTPRSRPPRLSDRARLALRMLPSELGLPLSWQQQQPHRRWHDSVQQLGLIILLLALMWFVRLYLHYCSQWLYLQAIAVPVNKFHFHAHTVDLIYQSSLLHTREELAMVVVGPLTLNAVTFLLVLIRWGCQLIFGSLPSFTSNFIMAQGVWTVLDPLAVFAVDAVLGHLAYSPDTPVGDAAKLYWHFYRADGSGAAGVIITLFLYAVHVLLSITILTIYLLRFHNDGRMLDVYQRLTAKEGVYFLPEDLELSNQELSYIVKKAEQWRGFNGERRKVAVYDSIWTAEDFLSSPSAPGDEPHTEDGLPPESETSTHVVIQTLYLSGLKQRYRHFLRQPDGTIIEVIGELHGLDNATKEVPSCLHQSSSRQREDKAGASISLQLRKRKKLFGRSNRVEPIAGSGCGSSFARLRLHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9E8</accession>
    <name>A0A3B5L9E8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000007437"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007532.1">
        <property type="protein sequence ID" value="ENSXCOP00000007437.1"/>
        <property type="gene ID" value="ENSXCOG00000005733.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157733"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000013">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000020">
        <property type="entry name" value="Dachsous cadherin-related 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000152">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000154">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000024">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000015">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000021">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000026">
        <property type="entry name" value="FAT atypical cadherin 1"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000032">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000037">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000041">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000051">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000052">
        <property type="entry name" value="FAT atypical cadherin 1"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000064">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000066">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000067">
        <property type="entry name" value="FAT atypical cadherin 1"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000071">
        <property type="entry name" value="FAT atypical cadherin 1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FAT atypical cadherin 3"/>
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    </dbReference>
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        <property type="entry name" value="FAT atypical cadherin 3"/>
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    </dbReference>
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        <property type="entry name" value="FAT atypical cadherin 3"/>
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    </dbReference>
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        <property type="entry name" value="FAT atypical cadherin 4"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000057">
        <property type="entry name" value="protocadherin Fat 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="30"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026">
        <property type="entry name" value="FAT ATYPICAL CADHERIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026:SF136">
        <property type="entry name" value="PROTOCADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="24"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <text evidence="10">Plays a role in coupling actin fibers to cell junctions in endothelial cells, via its interaction with AMOTL2 and CDH5. May regulate acid-induced ASIC3 currents by modulating its expression at the cell surface.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:signal transduction"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WW"/>
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        <property type="entry name" value="Membrane associated guanylate kinase, WW and PDZ domain containing 1"/>
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        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
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        <property type="entry name" value="MEMBRANE ASSOCIATED GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="17"/>
            <end position="100"/>
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    <sequence length="1253" mass="136632" checksum="5F87CB847481C2AF" modified="2018-12-05" version="1">MSKVIQKKNHWITKVNECAVIRDENGELNAELHGGAENGEFAYIGQIREDAVVYQDGKLSEGELLLEVEGLSVSGLPLYDIYTVINCCKGPVRLKTVRQGHKLNKDLKHFLSLRFQKTSPDHELQKTIRANLYRHAVPCTTRPPRDEEVPGLDYNFLSVEDFLQLEESGTLLEVGTYEGNYYGTPKPPRQPIIGTVTLSDAGSHPSTPKRTKSYHDMQKVHVVPADDDDDDQATEMNNSFTGEGNPNDQDESRSGILRPYPVRDNALPCGLNNAATSTAESGQQTLAEPQVSPEDPLGPLPENWEMAYTENGELYFIDHNTKTTSWLDPRCRDKAPRPLEECDDDECLCLMLCALCIPELPPGWERIDDPVYGVYYVDHINRKTQYENPVLEARRQKILEQQQPPQPPEEWIEEHSSAAAPLATLCSLDPSLSCCLLSLGGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLSLCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDAPTNQAGPINGMREPRPHSPLAEVASNGSHGYSSDVVTLASAIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGMCVVPAKRSPKLHQLERKDSQSSSQHSVCSHRSAHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPLQDPADGTLTLQKKPDPFKIWAQSRSMYESRREYISLVLLEEELVEQDIFLWRKDTGFGFRILGGNEAGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGVSKGEGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVIPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPQTTELRYILFLTPQDAEYYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKNMKHSRAIELIKNGGRRARLVLKRGDGSVPEYVICATYGKCNSNLFAFFKSLSLLPFLQTAAVTGTLPHPGHKTLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBE1</accession>
    <name>A0A3B5LBE1_9TELE</name>
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        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL38</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L38</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">RPL38</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eL38 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBE1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005194"/>
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        <property type="protein sequence ID" value="ENSXCOP00000005194.1"/>
        <property type="gene ID" value="ENSXCOG00000004072.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003718"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:protein-RNA complex assembly"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="60S ribosomal protein L38"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002675">
        <property type="entry name" value="Ribosomal_eL38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038464">
        <property type="entry name" value="Ribosomal_eL38_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10965">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10965:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL38"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01781">
        <property type="entry name" value="Ribosomal_L38e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="71" mass="8329" checksum="D68C1988D6F8DE7A" modified="2018-12-05" version="1">MNSNQFSSPRKIEEIKDFLLTARRKDAKSVKIKKNKDNVKFKVRCSRYLYTLVITDKEKAEKLKQSLPPGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBJ1</accession>
    <name>A0A3B5LBJ1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Islet cell autoantigen 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBJ1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009558"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009670.1">
        <property type="protein sequence ID" value="ENSXCOP00000009558.1"/>
        <property type="gene ID" value="ENSXCOG00000007276.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005530"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000015">
        <property type="entry name" value="Islet cell autoantigen 1, 69kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010504">
        <property type="entry name" value="AH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024114">
        <property type="entry name" value="Islet_autoAg_Ica1/Ica1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10164">
        <property type="entry name" value="ISLET CELL AUTOANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10164:SF3">
        <property type="entry name" value="ISLET CELL AUTOANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06456">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01015">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50870">
        <property type="entry name" value="AH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AH" evidence="1">
        <location>
            <begin position="49"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="238" mass="28102" checksum="636BD684EC1300D9" modified="2018-12-05" version="1">KETNKCKYLDRFIQNRDSSVVNKFQKKFWKTKQTLIKVTGKKEDEHVVASDADLDAKLEVFHSIQRTCMELLKVIEQYQRRICFLSQEENELGRFLRFQGSQDKSRAGKIMQATGKALCFSSQQRLALRKPLCRLYQEIETFRYRAISDTWLTVNRMEQSRTEYRGALLWMKDVSQELDPDTHKQMEKFRKVQAQVRTTKSSFDKLKNDVCQKVDLLGASRCNLLSHILSTYQVHLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCV1</accession>
    <name>A0A3B5LCV1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of very long chain fatty acids protein 4</fullName>
            <ecNumber evidence="10">2.3.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">3-keto acyl-CoA synthase ELOVL4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">ELOVL fatty acid elongase 4</fullName>
            <shortName evidence="10">ELOVL FA elongase 4</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Very long chain 3-ketoacyl-CoA synthase 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Very long chain 3-oxoacyl-CoA synthase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ELOVL4</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that specifically elongates C24:0 and C26:0 acyl-CoAs. May participate to the production of saturated and monounsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Oligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family. ELOVL4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.199" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCV1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010003"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010121.1">
        <property type="protein sequence ID" value="ENSXCOP00000010003.1"/>
        <property type="gene ID" value="ENSXCOG00000007588.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244838"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035338">
        <property type="term" value="P:long-chain fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03204">
        <property type="entry name" value="VLCF_elongase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033678">
        <property type="entry name" value="ELOVL4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF61">
        <property type="entry name" value="ELONGATION OF VERY LONG CHAIN FATTY ACIDS PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10 11" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10 11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="306"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="315" mass="36563" checksum="9E822FAFF882C17D" modified="2018-12-05" version="1">LRSLITSNGRAMEVVTHLVNDTVEFYKWSLTIADKRVENWPMMSSSSPTLAISCLYLIFLWAGPRYMQDRQPFTLRKTLIVYNFSMVVLNFYIAKELLLASRAAGYSYLCQPVNYSNDVNEVRIASALWWYYISKGVEFLDTVFFILRKKFNQVSFLHVYHHCTMFILWWIGIKWVPGGQSFFGATINSSIHVLMYGYYGLAALGPHMQKYLWWKKYLTIIQMIQFHVTIGHAGYSLYTGCPFPCWMQWALIGYAVTFIILFANFYYHAYRRKPSSHKGGKPVANGTSAVTNGHCNMEEVEENGKRQKKGRAKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFD8</accession>
    <name>A0A3B5LFD8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFD8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008401"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008507.1">
        <property type="protein sequence ID" value="ENSXCOP00000008401.1"/>
        <property type="gene ID" value="ENSXCOG00000006444.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183202"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000134">
        <property type="term" value="P:negative regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000049">
        <property type="entry name" value="Retinoblastoma-like 2, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002720">
        <property type="entry name" value="RB_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002719">
        <property type="entry name" value="RB_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028309">
        <property type="entry name" value="RB_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742">
        <property type="entry name" value="RETINOBLASTOMA-ASSOCIATED PROTEIN RB -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742:SF8">
        <property type="entry name" value="RETINOBLASTOMA-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01858">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01857">
        <property type="entry name" value="RB_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01368">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Retinoblastoma-associated protein A-box" evidence="3">
        <location>
            <begin position="99"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="2">
        <location>
            <begin position="484"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="605"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="731"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="333"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="608"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="774" mass="85892" checksum="BCE2385832C592B1" modified="2018-12-05" version="1">MEMQPSTPIKHVPPARLLRLSLSRLYEEHVLATGSLDERIFTGEGANENIGTPGPCFCDGMESQDSATYRLSTNLTASALKVSTPLTGRKYIQEVRMSSPLSYAMKSVGRLHTLLSGTKHGPSAKLTETLRGCARDPSDVISQRLKEMFDVYSQHYEDSELAVKYFHLAEALYYKILESIIEREKMILGDADLSCILEQDIFHRSLLACCLEIVTFSYRPPGDFPNVISIFQLPAYHFYKVIEVLVRSEQGLFREVVKHLNQVEEQVLESLAWTSDSPLWQSLRSAKVPSCQEVMPPQYLEQSEETNNGSIPLTPVKSGPDLRANSIVKGVSPSPTSLQDRYSSPPTGGFRRRLFVDPVEGEPSVTSTGSSALTSPPQTPIPKTGPSSIVTTIPAGQTVFTVTTNNGQTMTIPLQGIANESGSITLIPVQMGGATLQPLSAQTLTGTLTIQSPVTKPAAKAASSSLRKGSLSLFFRKVYHMASVRLRDLCAKLDISSELRRRIWTCFEYSVVHCTDMMKDRHVDQLLMCAVYVMAKVTKEDRSFQNIMKCYRTQPQASSNVRSHALSQDSILRFGSRPNSEDNNLPLHRLTSMWFSSPVPIHSSSTLSAPQPDSAASTPTSASDRNFPSSVGEDERGDLILFYNNVYIKHMRSFALRYSPSSPSTPPLCPYPTLRTGSPRRMLLSSKHSIYISPHRTGSSPLPTHRDRISYYICTSPPNRLQEINSMIRTGETPSRKRSIPLEEEASPKRVCPDSHTTLLRRLQDIANDRSSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFS7</accession>
    <name>A0A3B5LFS7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009702"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009816.1">
        <property type="protein sequence ID" value="ENSXCOP00000009702.1"/>
        <property type="gene ID" value="ENSXCOG00000007380.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155705"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05609">
        <property type="entry name" value="STKc_MAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000012">
        <property type="entry name" value="microtubule-associated serine/threonine-protein kinase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000012">
        <property type="entry name" value="microtubule-associated serine/threonine-protein kinase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037711">
        <property type="entry name" value="MAST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015022">
        <property type="entry name" value="MAST_pre-PK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023142">
        <property type="entry name" value="MAST_pre-PK_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF136">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED SERINE_THREONINE-PROTEIN KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08926">
        <property type="entry name" value="DUF1908"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140482">
        <property type="entry name" value="MAST3 pre-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="135"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="406"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="105"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="478"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="713" mass="81298" checksum="102179EA254A9BB2" modified="2018-12-05" version="1">GRSPVSFDHEIMMMNHVYKERFPKAHERSESEEVTFVTQLVKKLMIIIARPARLLECLEFDPEEFYHLLEAAEGHAKEGQGIKSDIPRYIISQLGLTRDPLEMTQLSSYDSGNPETPETDDSVDTKPKTPREEDFENIKLISNGAYAVYLVRHRETRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMFCSFETRRHLCMVMEYVEGDCATLLKNIGALPVDMARMYFAETVLALEYLHNYGIVHRDLKPDLLITSLGHIKLTDFGLSKIGLMSLTTNLYEGHIEKDTREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGVILYEFLVGCAPFFGDTPEELFGQVISDEIVWPEGDEALPQDAQDLIAKLLHQNPLERLGTGGAFEVKQNTFFTDLDWNGLLRQKAEFIPQLESEDDTSYFDRSDRYHHVDSEEEDTNDDEHVELRQFSSCSPRFSKLQVYSSMERLSLHEEKRTPPPTKRSLSEEGGDCVDSLSGLKTRDRSWLVGSPELRKRLSVSESSHTESDSSPPLTVRRRCCSAIIEMPRFAISAEEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRHRQTRGERRRPAVHQGHQVGVSQYAVAGDPRTPRSVAVGQPHVSTLAVVQSVVAGLLAQPRPFPGHQQRPTCHHHPPNREEVRLHAESHPRLHGRLQHLQRPSHGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFU4</accession>
    <name>A0A3B5LFU4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the apolipoprotein L family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFU4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008551"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008657.1">
        <property type="protein sequence ID" value="ENSXCOP00000008551.1"/>
        <property type="gene ID" value="ENSXCOG00000006542.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234599"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042157">
        <property type="term" value="P:lipoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008405">
        <property type="entry name" value="ApoL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14096">
        <property type="entry name" value="APOLIPOPROTEIN L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14096:SF61">
        <property type="entry name" value="APOLIPOPROTEIN L6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05461">
        <property type="entry name" value="ApoL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="146" mass="15677" checksum="A4B8185A4B0C6900" modified="2018-12-05" version="1">MHISADRVFMAARLFVRLFNQRGASLQQRILELLSLADLADDFHKKTVTASVGGGVASVAGSITTITGLILAPFTAGTSLIVTAVGIGIATAGGVTSASANITDTLHSKTDRKKVEKMIQDYQDEIKDIKDCLEFLQVRTCTHSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFW5</accession>
    <name>A0A3B5LFW5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">EEIG family member 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EEIG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFW5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011028"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011155.1">
        <property type="protein sequence ID" value="ENSXCOP00000011028.1"/>
        <property type="gene ID" value="ENSXCOG00000008332.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156132"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039931">
        <property type="entry name" value="EEIG1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019448">
        <property type="entry name" value="NT-C2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21456:SF3">
        <property type="entry name" value="EEIG FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21456">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10358">
        <property type="entry name" value="NT-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51840">
        <property type="entry name" value="C2_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 NT-type" evidence="3">
        <location>
            <begin position="3"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="227"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="238"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="266"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="343" mass="37385" checksum="ACA7FC8BECA3EB5F" modified="2018-12-05" version="1">MSFILMKKKKFKFKIDFDLEELSSVPFVNGVLFCKVRLLDGGFAQESSREPVQANCVHWKKRFSFICKMSANAGTGVLDPCMSRVSVRKLGFADLNLSEFAGSGSTVRRCLLEGYDTKNTRQDNSILKVVIHTQLMSGDPCFKTPPSTAMTLGIPPAEAGGLLEDRKGGDFRTSHSGAGTYIFIHSARQEILKSRSVPEEMIALSHSRTSSYASQLSKMSGYCSSYSSSTDLSHRRNSSGGSASTGIGSIEPGDQQGERGEKENRTTPTSTGSPALKQNSVENQLKRVDATRVDADDIIEKILQGQDFSHEEGLSLFVGPGGSTALGSQHMRWVWLCNTGYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGN3</accession>
    <name>A0A3B5LGN3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Myeloid associated differentiation marker a</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MAL family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027893824.1">
        <property type="nucleotide sequence ID" value="XM_028038023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGN3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008942.1">
        <property type="protein sequence ID" value="ENSXCOP00000008836.1"/>
        <property type="gene ID" value="ENSXCOG00000006738.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114157171"/>
    <dbReference type="KEGG" id="xco:114157171"/>
    <dbReference type="GeneTree" id="ENSGT00950000182933"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047123">
        <property type="entry name" value="MYADM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17068:SF3">
        <property type="entry name" value="MYELOID-ASSOCIATED DIFFERENTIATION MARKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17068">
        <property type="entry name" value="MYELOID-ASSOCIATED DIFFERENTIATION MARKER MYADM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="9">
        <location>
            <begin position="6"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="9">
        <location>
            <begin position="145"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="282" mass="31152" checksum="A83312C73D1378B5" modified="2018-12-05" version="1">MPVTWFPTSQLLWVRIAAVLFTCTAFSVVANGGWLPHGGMADWCIFSWAFSFACTLLVLLVEQCSLQSRIPVSWSNFPITVACYASLLCLSASIIFPVFFLKGQNLSQEVRDHRIASTVFSCLATVAYMVEVSLSKARPGEVAGYMKTAPGLMKVCQTFVACIIFILVSDPVSYDHHPALKWCMAVYCICFILSMAVVVLCVGECTGCLPVPFSKFLSAYGLLAVIMYLTATILWPIFQFDKKYSRWGDQTKLIAVAVLTGLNFLLYLADLAYSARLVFVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGY2</accession>
    <name>A0A3B5LGY2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">RAB39B, member RAS oncogene family b</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Autolysosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle membrane</location>
            <topology evidence="12">Lipid-anchor</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027893847.1">
        <property type="nucleotide sequence ID" value="XM_028038046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGY2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008931"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009037.1">
        <property type="protein sequence ID" value="ENSXCOP00000008931.1"/>
        <property type="gene ID" value="ENSXCOG00000006807.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114157185"/>
    <dbReference type="KEGG" id="xco:114157185"/>
    <dbReference type="CTD" id="566010"/>
    <dbReference type="GeneTree" id="ENSGT00940000158132"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120281">
        <property type="term" value="C:autolysosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04111">
        <property type="entry name" value="Rab39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000358">
        <property type="entry name" value="RAB39B, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041818">
        <property type="entry name" value="Rab39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="213" mass="24426" checksum="BA1C39C362CB56DA" modified="2018-12-05" version="1">MEAIWLYQFRLIVIGDSTVGKSCLIRRFTEGRFAQVSDPTVGVDFFSRLVEIEPGKRIKLQIWDTAGQERFRSITRAYYRNSVGGLLLFDITNRRSFQNVHDWLEEARSHVQPHSIVFLLVGHKCDLEAQRQVTRQEAEKLAGAYGMRYIETSARDAINVEHAFTELTREIFALVRSGDITIQEGWEGVKSGFVPNVVHSSEEVTKSDRRCLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIL3</accession>
    <name>A0A3B5LIL3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom40 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIL3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010480"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010603.1">
        <property type="protein sequence ID" value="ENSXCOP00000010480.1"/>
        <property type="gene ID" value="ENSXCOG00000007931.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003308"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07305">
        <property type="entry name" value="Porin3_Tom40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.10:FF:000005">
        <property type="entry name" value="mitochondrial import receptor subunit TOM40 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037930">
        <property type="entry name" value="Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10802">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="318" mass="35060" checksum="9A7BF6AEF964EA9A" modified="2018-12-05" version="1">MGSVLALSSNPGHQNWPFSKDHLPSRWDRRDGRLPNPGSFHSLHRSCKDVFPHQIEGVKMIINKTLSSFFKVSHTLHLSAVSPSYYRFHVEHLQSDDYSKDKDAPALIGEMDSSGSLNAHALLHLTERVRARTVFQTQQSQFVTWQFETEYRGSDFTAAVTVANPDILRESVILVAHFLQSVSSGLVLGGELVYHRGRAEEGGILTLAGQYSGPNWVATLNAGKGGAHASYYHRANKQIQVGVEFEASTRTQETTASFGYQMDLPEANMVFRGMINSRCIIGGVLEKRLTPLPATLIMGAFVNHKGDKLQVGLGVNVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJG6</accession>
    <name>A0A3B5LJG6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJG6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009870.1">
        <property type="protein sequence ID" value="ENSXCOP00000009756.1"/>
        <property type="gene ID" value="ENSXCOG00000007413.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286959"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001480">
        <property type="entry name" value="Si:cabz01071911.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000744">
        <property type="entry name" value="zinc finger E-box-binding homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000099">
        <property type="entry name" value="Zinc finger protein 79"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF5">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING PROTEIN 44 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="293" mass="33955" checksum="A8F2D517D1461D1B" modified="2018-12-05" version="1">DQEKPEPLQLKQEKEEPEPLQIKQEKEEPEPLQIKQEKEEPEPLQIKQEQEEPEHQHTTESSNQKLYSCKICNKIFPRKNSLPRHMRIHTGEKPFTCTTCGKSYRYKKNLIYHLKIYRGEKPFTCSICGKQYHLKDSLKNHMRIHTGEKLFTCGICGKSFPLNGNLSAHFKVHEGQKSFSCVTCGKSFRFQSHFLRHVRSHTGEKPFSCVMCGKSFTRRSNLAVHIREHMMVLRDAFFISALVVLSNVPQFRATMISRFKAFKLSIGQTLWSTGVCRNQWKVIITPTSGAVCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LK29</accession>
    <name>A0A3B5LK29_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Bone morphogenetic protein/retinoic acid inducible neural-specific 3b</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BRINP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LK29"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009956"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010074.1">
        <property type="protein sequence ID" value="ENSXCOP00000009956.1"/>
        <property type="gene ID" value="ENSXCOG00000007554.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165058"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071300">
        <property type="term" value="P:cellular response to retinoic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045930">
        <property type="term" value="P:negative regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045666">
        <property type="term" value="P:positive regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033237">
        <property type="entry name" value="BRINP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057671">
        <property type="entry name" value="BRINP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057450">
        <property type="entry name" value="BRINP_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15564:SF10">
        <property type="entry name" value="BMP_RETINOIC ACID-INDUCIBLE NEURAL-SPECIFIC PROTEIN 3 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15564">
        <property type="entry name" value="MACPF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19052">
        <property type="entry name" value="BRINP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25415">
        <property type="entry name" value="EGF_BRNP1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0338">Growth arrest</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="MACPF" evidence="7">
        <location>
            <begin position="56"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="624"/>
            <end position="645"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="756" mass="85040" checksum="B9AA9140110C1EF1" modified="2018-12-05" version="1">LVLQCYDFILAILHFILGTLQLSSHTVTTLIMFHKISQYCDFILIQLYSHDTTSLFSEFGRWKVNSLAVEKRDSFGGSGGLALPLDPDLMHTIRQLGRRPTLQMITESLIKKYGTHLLLSATLGGEESLTIFVDKRKLSLESSPVGADGGGGRGSNTTGAVTLEALHQLAASYFTDRESTLRRLHHLQIASTAIRVTETRTGPLGCSNYDNLDTVSSVLVHSPENKVQLQGLQVILPGYLQSRFIQAALNYIGCKSEGQFVCRNNDCWCECGGDFPQCNCPHSDLDTLENSLLHIRDALRLSNKDFEESEEFRGFVGKLPVHYAVNTSVVEHLWRTDGGLLQRHRQLETSSKQLLARARRTTNKLFSLSRRCRKPPRIILPRSLHFWLNCALSTLYCSENKQVGVYSERSRSCSCPYDHPPCQGPLPCSVGDGPRCASCSAENRTRCAGCNPGFTLTQGACRPAVPDPTDPYLGMESDRDLQDLELRFLMQRRDPRLALHGVFVSNDVRINTWFDPSWRKRMLLTLKSNRVKSNRVHLLLGLALQFCLTRNSTLEPTLSLFVNPFGGSHSESWTMPIGQHGYPDWERTRLDIPLDCYNWTLTLGNRWKSFFETVHFYLRSYSRDSAGGSKNASLYSDPPEDPEPSSNVGYMKVNSMQLFGYSVHFDPEAIQDLILQIDYPYTQGSQDSALLQLVELRYRVNRLSPPGSPHVDLFACLLRHRLKLSGADVARILTALQAFSAKHPNYTEYEANKLCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMI7</accession>
    <name>A0A3B5LMI7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMI7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009069"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009179.1">
        <property type="protein sequence ID" value="ENSXCOP00000009069.1"/>
        <property type="gene ID" value="ENSXCOG00000006915.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155231"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045180">
        <property type="term" value="C:basal cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22713">
        <property type="entry name" value="FHA_PHLB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000004">
        <property type="entry name" value="pleckstrin homology-like domain family B member 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052212">
        <property type="entry name" value="PH-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12156:SF23">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY-LIKE DOMAIN FAMILY B MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12156">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY-LIKE DOMAIN, FAMILY B, MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="1">
        <location>
            <begin position="65"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="173" mass="18990" checksum="623390F17EA42D30" modified="2018-12-05" version="1">MDRMRRNKVEHERQTHQVLQNTPLDLIETGKSLKVQAERPHLVSLGSGRLSTAITLLPLLEGRTTLGSGGTDIPLQGPGIAAQHCYIENQGGNITLYPCGNQCSVDGLPITKPYRLTQGCMLCFGQSAFFRFNHPEEAQRMKSMLPAGSLRVNTVKPLPSGKKNKQTKNINSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LN84</accession>
    <name>A0A3B5LN84_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LN84"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013553"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013720.1">
        <property type="protein sequence ID" value="ENSXCOP00000013553.1"/>
        <property type="gene ID" value="ENSXCOG00000010267.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158602"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44464">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44464:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="42"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="100" mass="10791" checksum="11F90154DFA2DC54" modified="2018-12-05" version="1">EPGLLSAARSQMGLPERLGTPPAERLAGQEASRFALTSVFKFQGHVETIFDCKFKPDDPNLLATASFDGTIKIWDTNTLTAVSTSPDSKRIATCSGDGFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQN6</accession>
    <name>A0A3B5LQN6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dolichyl-phosphate beta-glucosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.117</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Asparagine-linked glycosylation protein 5 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-alpha-D-glucose = a di-trans,poly-cis-dolichyl beta-D-glucosyl phosphate + UDP</text>
            <dbReference type="Rhea" id="RHEA:15401"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19502"/>
            <dbReference type="ChEBI" id="CHEBI:57525"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.117"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15402"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.117" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQN6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013357"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013522.1">
        <property type="protein sequence ID" value="ENSXCOP00000013357.1"/>
        <property type="gene ID" value="ENSXCOG00000010123.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153481"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004581">
        <property type="term" value="F:dolichyl-phosphate beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04188">
        <property type="entry name" value="DPG_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000068">
        <property type="entry name" value="ALG5, dolichyl-phosphate beta-glucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035518">
        <property type="entry name" value="DPG_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="15">
        <location>
            <begin position="35"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="289" mass="32878" checksum="A296D2FF4226BABF" modified="2018-12-05" version="1">MVNLTCHENEKYFLTATGERKPFPSLHDPFSRELSVVIPAYNEELRSDISVKIPLIISLQKQNPSFTYEVIVVDDGSRDKTTEVALRYTNKYSADKVRVLTLVKNRGKGGAVRMGTLSSRGKLILMADADGATKFSDLEKVEAGLKDLNTGPENLAISCGSRAHLEKDSVAQRSVFRTFLMYGFHFLVWFFCVRGIKDTQCGFKLFTREAALKTFSSLHVERWAFDVELLYIAQCFKIPIAEVAVNWTEIEGSKLVPFWSWLQMGRDLIFIRLCYFTGAWKLHSPHKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQU6</accession>
    <name>A0A3B5LQU6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000013165"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013326.1">
        <property type="protein sequence ID" value="ENSXCOP00000013165.1"/>
        <property type="gene ID" value="ENSXCOG00000009975.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161664"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47166">
        <property type="entry name" value="ZINC FINGER PROTEIN 831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47166:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="157"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="95"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="173"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="298"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="452"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="840"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1085"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1248"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1384"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="228"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="298"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="362"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="452"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="847"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1091"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1109"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1129"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1140"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1248"/>
            <end position="1258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1429" mass="154470" checksum="3D1456E846582CA4" modified="2018-12-05" version="1">METGKPTLSSVPVHTGSVRAQAEKRMDIQAPLTAVYIQTVPAVPMQPFPQTPADAREANALHLAMPPLYSKETLPFLTLHLSGGLQPQLGLSAAAAPAPAPAPAPAPAPAPVPTPTPTPAAKPKSAGKHVCPHCGRDCMKPSVLEKHLRCHTGERPYPCTTCGVSFKTQSNLYKHRRTQAHARQSSESEQSNLGSLDSMSSSRETCSSSLSLDEQCEDLGSEEKHATLSATESPTPAVWTQSSKQSETSPTVPRAETNGSDKLTVSGDKQKVENTKESLTISRHLQLQRQEATLFSKQWERSVSRGKSQSHESTDSGFSESSDHYPSPSSVLPEHCVDSLSKPSREHLEETQNTHLCSEQGHGGRDEKDAAREQVHKRLEERISKLISDNSAVVEDKHLENVRPRKTVLSKQGSIDLPMPYTYKDSFHFDIKINPAQNTGMQRHTKSGLFNSVPTQCSSTTEHTPLTRSSSLPFSVTLLQPDRSSPTSSSQSDYATDIGRGSSGQINPTGLLKKPVNQHSSSHRPLVRQTAIDCNHTTDSLLTTSSVEEACTGSLSCDRDASDIIGEPGSRKFRRKKAQKFAYSKWYMYGGGTFKKLYDSDKSGDSTALKGRKCLTNLVHEAAQGVQKRVSMLQKETVTRSGSTVNFSTSSATAGHLSANLSLVSSVDLNVERCCLHSTNGSAKAPLTRHLSLSVLPSSATRSVPVHKTECFNRTEAGRLRDVPKHSDSTTELCEAHVPSDRKKQKTGEKIISPLEMEIAPNTLTQHPVSVTVSTPTQGTDLTYVQAKEIPNSPGLKGTLFLPCLLNANVSQIGTSATTSVSVASKSSFLPKYQLKLPNAHEPGSVSLPQAQSKPKGTDDCSPSPSDQTLVTSYENISKNTLPSMQTQDHLAFSRTVTSICQVKTSQHTHSLSSNVSAMQKQLAATTITTSCFQDYSSGLCSALLHCSKTATDSMHKHLPRPLATAAPSVSLPTGNNQTSAATITGLSHHLSNHSSNTLGDRQLSTASILANPTNKANVDSNASVVRCRIVPLDQQAQNVFHVHTADLQICLQIISDEQMALIAPQIERQSGPSLSQLREIEVVTPEGAPSKSQTFDSMKMSNATEGEQQPKDLKEGVRQEHLSRLNSERASPPQSVQLVKEKTEHSDAYRAASSTKSFPPQPPSRHLHNSGHLITVTQTRNSVGSIMATSASVRGENSGGSHTSEAKQVLFPNTFAEEKFLSTKRVNQIGMASQMLTGQQKICERSFSTSPANQSSMKIEPLDKSQQKAKHPGASCNANVLNSQDGALACKISRTESGEFLKQANSDAGIDASVSQLSNSNPNVVRQLNPQAFVFHSHQMEPAKSTSQSSLKHANLHLSRVELSDNSSSSMEYCPQEIQNAMRREHSEKSPGQFNLQPASVDMKRPGDVTSFQSSTTGMNYSLIMLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQW0</accession>
    <name>A0A3B5LQW0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQW0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013432"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013598.1">
        <property type="protein sequence ID" value="ENSXCOP00000013432.1"/>
        <property type="gene ID" value="ENSXCOG00000010186.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000174817"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015026">
        <property type="term" value="F:coreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042288">
        <property type="term" value="F:MHC class I protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050776">
        <property type="term" value="P:regulation of immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042414">
        <property type="entry name" value="CD8B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11292">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD8 BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11292:SF7">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD8 BETA CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="197" mass="22381" checksum="DF6893774A6EEDD8" modified="2018-12-05" version="1">FTLNHISFLKSIECDCGKLSCDSVYWFRTVPSESKAEFIGRSNNADRSNHGPSYEADKHRFKLSKRGSSTFVLRIINVTKADAGIYSCIVTASKNMTFNPGVVFRPGGYKSQSLLLWENIRITKHFIIKCHIFFSFFFNPSNCFSLFFPNRIAQKMSPPLCEVSPDVKLLASFYKKKQNGFISSISIGILFRCITIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRL4</accession>
    <name>A0A3B5LRL4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRL4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010434"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010557.1">
        <property type="protein sequence ID" value="ENSXCOP00000010434.1"/>
        <property type="gene ID" value="ENSXCOG00000007905.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155989"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047149">
        <property type="entry name" value="KIF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970:SF29">
        <property type="entry name" value="KINESIN FAMILY MEMBER 20B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="82"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="166" mass="18568" checksum="769EDF33E56E7F85" modified="2018-12-05" version="1">MKITFLLACVTIEGPNSVVIRAPRFCQPNRQGDKSLPQTAQRFTFTQVFDPDASQRTVFEGSVRGLVQDALQGGNCLVFTYGVTNAGKTFTFLGPDHNSGLLPRSLSVIFNSIKGHQYSRSDLKPQRCRDFSRLTPEQQAAEISTKKNLLRLLKEVGHEFCYHDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSB8</accession>
    <name>A0A3B5LSB8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein AAR2 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">AAR2 splicing factor homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the U5 snRNP complex that is required for spliceosome assembly and for pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with PRPF8 (via RNase H homology domain). Component of a U5 snRNP complex that contains PRPF8.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAR2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027880453.1">
        <property type="nucleotide sequence ID" value="XM_028024652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSB8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010704"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010828.1">
        <property type="protein sequence ID" value="ENSXCOP00000010704.1"/>
        <property type="gene ID" value="ENSXCOG00000008078.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114149080"/>
    <dbReference type="KEGG" id="xco:114149080"/>
    <dbReference type="CTD" id="25980"/>
    <dbReference type="GeneTree" id="ENSGT00390000007796"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13778">
        <property type="entry name" value="Aar2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13777">
        <property type="entry name" value="Aar2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.550:FF:000001">
        <property type="entry name" value="AAR2 splicing factor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.20:FF:000001">
        <property type="entry name" value="protein AAR2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.550">
        <property type="entry name" value="Aar2, C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007946">
        <property type="entry name" value="AAR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033648">
        <property type="entry name" value="AAR2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038514">
        <property type="entry name" value="AAR2_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033647">
        <property type="entry name" value="Aar2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038516">
        <property type="entry name" value="AAR2_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12689">
        <property type="entry name" value="A1 CISTRON SPLICING FACTOR AAR2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12689:SF4">
        <property type="entry name" value="PROTEIN AAR2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05282">
        <property type="entry name" value="AAR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20981">
        <property type="entry name" value="AAR2_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="AAR2 N-terminal" evidence="10">
        <location>
            <begin position="20"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="AAR2 C-terminal" evidence="9">
        <location>
            <begin position="211"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="387" mass="43704" checksum="3FA08AD08C470AFA" modified="2018-12-05" version="1">MAGSSCVDMDPDVALRLFEEGATLVLLGVPQGTELGIDCKSWQVGPRFRGVKMIPPGLHFLHFCSVNSPSCGGEIGPKSGLFLTLKPREILLAHWDPKEEDLDFSASKNDEEVNRIRSTLRELDPYLGPYPYEVMRKWVSLTDQISQELAVNLQPLSGRVCAFSDIIPELQFRHTKDRAEQPRNDTACQSMREGLDRLPKMKQREGTELRFSDIPKKTYPPGASPAEITRCSMDLSYSLETVVEQNYPDQPLNLLGELQFAFVCFLVGNVYEGFEHWKSLLALLCRSEDAMRKRKELYLGLITVLYHQLGEVPPDFFVDIVSQSNFLTSTLQDFFQFATGPGVDSTLRKKAEKFKAHLTKKFRWDFDADLDDCAPVVVEIPEGVTVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTI2</accession>
    <name>A0A3B5LTI2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proline and serine rich 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTI2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015313"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015504.1">
        <property type="protein sequence ID" value="ENSXCOP00000015313.1"/>
        <property type="gene ID" value="ENSXCOG00000011603.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01010000222533"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16095:SF9">
        <property type="entry name" value="PROLINE AND SERINE-RICH PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16095">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 143 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="41"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="115"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="161"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="181"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="267"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="385" mass="43111" checksum="5EE309FD82BBA057" modified="2018-12-05" version="1">MVFVWYKNNFLNVLQIFREEQECLQYFEETIESLEDGIDEEQQRLGQRSRPELNSRPFDSVGRHGSLAPNSEVSPNYIIDLVHSEPDLVHAAEPVYSPAHQDFYHMPYTPESNFEAKPRHDVFPSEYNPPLRSASFGTTDSHSSYHPPGSVPTPVLIAQQLAENQGDGTSNSHPSSLLRRLSMESEKTPRDRSNISVKHGPGPSSKPAHYPPNIHVVHGGKEPQIPVANVNIHERQEQMLAHLAGTSHPLLQHIEDDTRNSPSRSISFKDPEPDKSRMEALSKLGLTRGRLPAGPFISNNVPSSKSRAKSVKPPPPTPAPRPPRQSYHGMLPPQKPPARASSPEYRRRPSSMFRPQGITVQFCGRGAMNESRREALRRLGLLKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTU4</accession>
    <name>A0A3B5LTU4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DnaJ (Hsp40) homolog, subfamily C, member 27</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027866527.1">
        <property type="nucleotide sequence ID" value="XM_028010726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTU4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014270"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014447.1">
        <property type="protein sequence ID" value="ENSXCOP00000014270.1"/>
        <property type="gene ID" value="ENSXCOG00000010812.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114140618"/>
    <dbReference type="KEGG" id="xco:114140618"/>
    <dbReference type="CTD" id="51277"/>
    <dbReference type="GeneTree" id="ENSGT00940000157133"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04119">
        <property type="entry name" value="RJL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000019">
        <property type="entry name" value="dnaJ homolog subfamily C member 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000697">
        <property type="entry name" value="DnaJ homolog subfamily C member 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="217"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="273" mass="30868" checksum="B1BA8A6C4D96AA9B" modified="2018-12-05" version="1">METTATKRRDNKKSLRVKVISLGNAEVGKSCIIKRYCEKRFVPKYLATIGIDYGVTKVQVRDREIKVNIFDMAGHPFFYEVRNEFYKDSQGVLLVYDVGLRESFDALDSWLGEMKQEMGSQANMDSIVFVVCANKVDLTKRRVVDEGEGRLWAESRGFHYFETSAQSGEGINEMFQAFFSSITDMCENGGKRPVSEVSVGFTKEQADTVRRIRNSKDSWDMLGVKPGATREEVNKAYRKLAVLLHPDKCVAPGSEDAFKAVVNARTSLLKNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUN1</accession>
    <name>A0A3B5LUN1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Adhesion G protein-coupled receptor F7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUN1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013176.1">
        <property type="protein sequence ID" value="ENSXCOP00000013016.1"/>
        <property type="gene ID" value="ENSXCOG00000009855.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154603"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000058">
        <property type="entry name" value="Adhesion G protein-coupled receptor F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051587">
        <property type="entry name" value="Adhesion_GPCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45813:SF4">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45813">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="378"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="416"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="9">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="10">
        <location>
            <begin position="174"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="454" mass="50377" checksum="580C2078922FBAE5" modified="2018-12-05" version="1">MMILFSVLSNNNRNSTFITETFLFLEDLKNATVRNNDLIINVYNSSVDIEIPQAGGEEKFITIIIFASMYNVLPGRDQLNTSTNRVHGRVALVRPEVEISNISLTFDVINYTNDTIGNPKCVFWDFSLYDGLGGWSDNGCSLVYFENETVSCNCNHTTSFSILMSPDSPRSLILDYITYIGVGISMACLVICLIIEAIIWRKINKNATSYLRHVSIVNIALSLLIANIWFIIGAAISDADQKNPPACTAATFFIHFFYLALFFWMLASALLLLYRTLSVFDGGLSKLTMLIIGFSLGYGAPLIIVTITIAATAPSNEYIRENVICWLNWYESKALLAFVVPALLIVVINFVILIVVLYKILRRRVGGNAAQTGEKHVLYVIARSLAVLTPFFGLTWGLGVGVMVSPQNFGINVAFAFFNSLQVGILLLCPLLCSSVLSLKKKLIMENSHMCGRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVT0</accession>
    <name>A0A3B5LVT0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">3-hydroxybutyrate dehydrogenase, type 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027860432.1">
        <property type="nucleotide sequence ID" value="XM_028004631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVT0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014975"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015163.1">
        <property type="protein sequence ID" value="ENSXCOP00000014975.1"/>
        <property type="gene ID" value="ENSXCOG00000011352.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114136578"/>
    <dbReference type="KEGG" id="xco:114136578"/>
    <dbReference type="CTD" id="622"/>
    <dbReference type="GeneTree" id="ENSGT00940000156929"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000074">
        <property type="entry name" value="Retinol dehydrogenase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313:SF36">
        <property type="entry name" value="D-BETA-HYDROXYBUTYRATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE FAMILY 9C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="361" mass="40133" checksum="A018C0B1F5F1FBC4" modified="2018-12-05" version="1">MAPQSAFRVTLLVSFSVLLTVVLGFGLPALLNAIMRLLGLPETSVTECIVVIYATFVLYVATPRFPRGSVEVQGKAVLITGCDTGFGLSLAKHLHTLGYTVFAGCLLKDKDGEGAKELEEFHSERMKVVQLDVCSDDQVKKAVEHIKGNLEDSQRGLWAVVNNAGVSTFGEVEFTSMDTYRQLSEVNLWGTIRVTKAVLPLIRNAKGRVVNIASMYGRMGNRLRSPYCVSKYGVEAFSDCLRYEMKTWGVKVSVIEPGNYIVATGILTRDIVATTANKLWNEAPAEVKDDYGKSHFEQYMALMRSYCNSGEKDMTPVLDDIADAIGSKRPYTRYSPMEPHWWIRMQVMTHLPGALSDLLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LW70</accession>
    <name>A0A3B5LW70_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Discoidin domain-containing receptor 2</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">CD167 antigen-like family member B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Neurotrophic tyrosine kinase, receptor-related 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Receptor protein-tyrosine kinase TKT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Tyrosine-protein kinase TYRO10</fullName>
        </alternativeName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="20">Binds hydroxyproline-rich sequence motifs in fibrillar, glycosylated collagen, such as the GQOGVMGFO motif, where O stands for hydroxyproline. Interacts with SRC. Interacts (tyrosine phosphorylated) with SHC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LW70"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012089"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012227.1">
        <property type="protein sequence ID" value="ENSXCOP00000012089.1"/>
        <property type="gene ID" value="ENSXCOG00000009136.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154842"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    </evidence>
    <sequence length="820" mass="93146" checksum="D34D1476E7C4322D" modified="2018-12-05" version="1">MHIFDVPGVCRYALGMSSGQIPDEDISASSQWSESTAARYGRLDGEEGDGAWCPEITVEPDHLKEFLQIDLRSLHFITLVGTQGRHAGGMGNEFAQMYKIKYSRDGSRWISWRNRQGKQVIEGNRNTYDIALRDMQPPIIARLVRFMPVIDHSMNVCMRVELYGCEWLDGLVSYNAPAGQQMNLPAYTVYLNDSVYDGAVIRSMTEGLGQLTDGVCGEDDFTLSHVHNGWPGYDYVGWSNESFPGGFVEIMFEFDRIRNFTSMKVHCNNMFSRHVKAFQQVVCHFRSDSDWETTPLAFSPVEDEKNPSARFITVKLANHMASAIKCQFYFADAWMLFSEITFQSDTAMYNTTPPSTPKPDDPTHKVDDSNTRILIGCLVAIIFILVAIIVIILWRQVWQKMLEKASRRILDDELTASLSIQSETFSYNHTGNRPGTAGEQESNSTYERIFPLGPDYQEPSRLICKLPEFAQSSEEPGKPDGAPHYAEADIVNLQGVTGGNTYAIPALTMDLLSGKDVVVEEFPRKLLAFKEKLGEGQFGEVHLCEAEGMQEFINEDYLFDVPEGQPVLVAVKMLRSDANKNARNDFLKEIKIMSRLKDPNIIRLLAVCIYSDPLCMITEYMENGDLNQFLSRHEPEGQLALLSNAPTVSFSDLCYMASQIASGMKYLSSLNFVHRDLATRNCLVGKNYTIKIADFGMSRNLYSGDYYRIQGRAVLPIRWMSWESILLGKFTTASDVWAYAVTLWEILNFCKEQPYSQLTDEQVIENTGEFFRDQKRQIYLPQPVLCPVSLYKVMLNCWRRNAKERPSFQELHRALVDLQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXC4</accession>
    <name>A0A3B5LXC4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Otolin 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXC4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016563"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016777.1">
        <property type="protein sequence ID" value="ENSXCOP00000016563.1"/>
        <property type="gene ID" value="ENSXCOG00000012497.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000001">
        <property type="entry name" value="Complement C1q B chain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
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        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF51">
        <property type="entry name" value="OTOLIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017427202" evidence="8">
        <location>
            <begin position="24"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="9">
        <location>
            <begin position="348"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="184"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="184"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="485" mass="51322" checksum="403D74B0BC32D869" modified="2018-12-05" version="1">MRIISCQWTLVAVAVVSITVAFCTKTKPTAKPKYLYTKKPAPHVTPHNPVTTSVPKTLKIVVTPNPVQPQPQPPPPPQPTAVRTTYMNHNLPQHYSENTEPTGVGPDNYTLDYNECYFNFCECCPPERGPRGSKGERGSAGIKLNEVVNGIILHKNTCVSGDIGPAGPQGIPGPPGMSGPMGFKGDKAGLKGEKGDVGFQGVKGKQGEKGDHGQNGTAGEKGEPGLEGPVGPPGTAMGQGPKGEKGDKGDCGIFGERGPKGDRGETGAPGIQGAMGIPGLHGKHGTPGPVGIRGDPGHPGPPGEPGVSGPQGPQGIRGMPGPKGDRGYPGIRGERGFRGLKGAKGSGMLQKRSAFSVGISPKKSFPPSGFPIRFDKIFYNEENHFNMSSNSFTCVHPGVYVFSFHITVRNQPLRATLVVNGLRKVRTRDSLYGQDIDQASTLVVLRLAAGDQVWMETFRDWNGVYASSEDDSIFSGFLLYSDKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZ08</accession>
    <name>A0A3B5LZ08_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
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            <taxon>Metazoa</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSXCOG00000012235.1"/>
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    <sequence length="1523" mass="168040" checksum="E4C37C9C3DD9EA42" modified="2018-12-05" version="1">MAKLCPHHQRRIVDAIGFLQTEVKAHASSNVQKTSDSTSGVRGKASLNSNSSSTIPEKSHPELKLPNKSTPKAEVLEFPNSVPNSDSQKNILEDAVSLKTSITPGPPLDVFSLGYGSNQPQVSSTPSSVDGENNRQSDQPPVRMKIKTSSAAAGKKLSCVLDNPLSSPFHFLEEKRGSSNRTETHSARLSSSVKRHSQLNVTPQASQKEVLEHQKDKMAKVFPTHVSFPSNSIRTARKTVRVSSEHQVRDNACREIVDPDIGNCDIVFIDKPITDCLKEKRRGMAPRRNARKSTRGHMYSDDIWELKTVRTLAGRGNCPNPMPEMTTLVTPKQILTKPEGLPPVDMPFAGTFSETVNQQIPSEESNEHVISGAGDVVEVAASKVDSVEVEMSQTDQGQNKVQSGSPTFMNLLVESQETLSDTLTDQETMNDLRMAKEIGESVGPPSYESTTSNEPESQQVVLDNTEQTVTETQVDSLESLTPGEAEHQLSSDKLLSCEDDIQNSSVTVNLVEEETKKDKEEKTQEEQSQEIQPQMELHCDNVERTEMSVSTGIAEELTVRRIETEMPETKNCVEPVETTNNEAECNEPAKPVDELPKELPPWHRKKDTKAQLPDQLRQPEPVIVGFVNGKPVSASDRSLRHRADRNPTPSSKTPIKSCQNLLMCSVSDSTPTSAVENKKLEEPQLETNTLETAMIQQVMEPSSDLLSSETSKLPSRTKETPKQKSSPRNQGRLPVPSDQSADQTESTVSKRQLRSDTQNSASISSIITSISSPKPSTLILPPAEQLPSLRIPPFPLPLTISSLNKHSDPSVSECSQQHKVSETMRPNTKNTQPIKEISEDTQKNKLVSESETKQTLRSGKVVSDNSKNEKHLSTEELNSSEKPSPVKTENQTEFVPTSNKRGLRKNSDVGESDLIQKQNVILVEDRLASEDKDVSKSERPRRMPLRSNTEMSDEAVSQSPPPDNKKLVLRSQRSTPHFTSAADTPKQNDVVSPVRIIPERTAKAQLKPTSGTVAPLTHSAQTPVIAPKQEPPKQTVNKFFQALTGEENQHLITNLNTKYDKMLKGWVQMDKEGQPTAKYKNKSDRQAAIWKSKRRARKPKSSEQQKYSPVQMLFMKGFSLTSICRWFLDSTETKSLVIVKKVNTRLPSETQLCFHSSSTGSGSAQDAFPSLQAERLKKHLKKFPIASPVKSNPRSQKLIAKALEQETNTVKRAELPSSTQNLSKYSAAKASAQKGESQKPSGKSKNPASARILRKYSNIRGKMQGQQSTVRMKRGSKMLKNKKSVVVTKSSAKSNLNPRLKMKKSTVPVSKKIKESAAKLARRKILTGSKAAKPSLPKRAVKTQANRVSKDKSKTKLPKRCSQRLGSPKVPEYQPVDTSKSKISSKKIYEVEREVSKKKPQTKESAHSTIVESKGKEIAAETPQQGVDVKASGSSDQVQTRSQRKMEAATPLSGSLGNHSKRAKKTSLVESPKAATELDEPKLTRSGALKRSQASSLPRSGTKVATKRAQELQETPAKRTRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZH1</accession>
    <name>A0A3B5LZH1_9TELE</name>
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        <submittedName>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
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        <subcellularLocation>
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    <keyword evidence="5" id="KW-0677">Repeat</keyword>
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    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
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            <end position="603"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1069" mass="115387" checksum="77A53C4C80A41F09" modified="2018-12-05" version="1">MTTANCPGNEELDFRLIFGEDGQQQPLGPADLEPDDNTSFYILNMGQPQAMVTNHQSIGLPRHGMHTHPQVISTSPRLPSHKTGTDLLSYEAQGSKYGTSPLLPSAPVEAPKAFECPSIQITSISPSCQQELDASEGELTANGPDGDYHGDRPLSRDHLYLPLDHSYRDSSLSPSPCSSLSSRSWFSDASSCESFSHVYDDVDSELNEAAARFSLGSPLTSPVCVSPQAVGGVLEEQSPWQHHHPPFVHHSHSTLSPRQSPCHSPRNSVTDENWLSPRSPSRPSSRPTSPCGKRRHSSADICYPGSLSPHHSPTPTPGHSPRGSVTEDTWAGSPTIGMSAFQCCPSETDIPSKTRKTSQDRTSLQTGKGELALDDQENLSSNLDSPPDDTLHSMKKDGPAEQFLSVPSHFSWNKPKPGHTPIFRTTSLPPLDWPLPSQFGQYELKIEVQPKAHHRAHYETEGSRGAVKAASGLTSVYFLSLPLSVVMFIGTADDRYLRPHAFYQVHRITGKTVATASQEILLSSTKVLEIPLLPENGMSASIDCAGILKLRNSDIELRKGETDIGRKNTRVRVVFRVHIPQPNGKVLSLQAASIPVECSQRSAQELPQVEKFSLTSCLVSGGEEMVITGSNVFPESKVIFLEKGPDGRPQWEVEAKIIREKTQGSCIVVEIPPYHSKTVTSAVQVQFYVCNGKRKRSQSQRFTYLSVMVKQEHRDELDLTAVPPISMPLAHAPSLVHPQLPSPDQGHPSDNLMSSSLHSLATGSGPGLLPLPGPCSSLSSPPFQHLPHLQGHSLHHPPADCHMTFHQGSAPAPPRPTYQPLQHSPSHNLSFNGQPSLSSQSYERMPFQQSPTAASHPIGLGIVYSSSCSSPSALSSSPTNPIVGSPQNHQPMLSPSSPHLHTLGGFHCSPNPSQVPSQGGHPLSGQHSSQLQRGLGYHPVSQRSASCPTPSTAPQQRMIPSPHSGPSSPQVHPLPYQSPPSSSPTSAGSPLGPLQQHSGQPSPQATSPVMTSLSPAAVGPLVHPLSPQGPFPSEGERLNIKQEPEEREPTFRSIGLQDITLDDGKRNGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M112</accession>
    <name>A0A3B5M112_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial ribosomal protein 63</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027863574.1">
        <property type="nucleotide sequence ID" value="XM_028007773.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027863575.1">
        <property type="nucleotide sequence ID" value="XM_028007774.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027863576.1">
        <property type="nucleotide sequence ID" value="XM_028007775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M112"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017783"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018007.1">
        <property type="protein sequence ID" value="ENSXCOP00000017783.1"/>
        <property type="gene ID" value="ENSXCOG00000013394.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114138480"/>
    <dbReference type="KEGG" id="xco:114138480"/>
    <dbReference type="CTD" id="78988"/>
    <dbReference type="GeneTree" id="ENSGT00390000008171"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005761">
        <property type="term" value="C:mitochondrial ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016576">
        <property type="entry name" value="Ribosomal_mL63"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14520:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14520">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN 63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14978">
        <property type="entry name" value="MRP-63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="12605" checksum="423E2381EB70E650" modified="2018-12-05" version="1">MFLTLMLLRKGIPGKQWIGKHRRPRQITWQMKSKMVEHLEREAETEYWLSRPYLTKEQEVRHAAERRAQTWLQIKEPNFANFPEHKRITDHLSHLRITKTWSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M263</accession>
    <name>A0A3B5M263_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M263"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017601.1">
        <property type="protein sequence ID" value="ENSXCOP00000017380.1"/>
        <property type="gene ID" value="ENSXCOG00000013102.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155929"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="101" mass="11526" checksum="DC263B8AD16F7877" modified="2018-12-05" version="1">MAAAIASSLIRQKRQARESNNEKVATNKKRSSPSKDPRSLCERHIFSVFSKVRFCSGKKRPVRRKPVCMYKEPSLHELEEKLLKSSRSPTLNSGHVSMNQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M355</accession>
    <name>A0A3B5M355_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Low-density lipoprotein receptor-related protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="18">Homooligomer. Interacts with MUSK; the heterodimer forms an AGRIN receptor complex that binds AGRIN resulting in activation of MUSK. Interacts (via the extracellular domain) with SOST; the interaction facilitates the inhibition of Wnt signaling. Interacts with MESD; the interaction promotes glycosylation of LRP4 and its cell-surface expression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDLR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000018578"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018811.1">
        <property type="protein sequence ID" value="ENSXCOP00000018578.1"/>
        <property type="gene ID" value="ENSXCOG00000013983.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158287"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000029">
        <property type="entry name" value="LDL receptor related protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000017">
        <property type="entry name" value="LDL receptor related protein 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000092">
        <property type="entry name" value="LDL receptor related protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000009">
        <property type="entry name" value="Low-density lipoprotein receptor isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000009">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000034">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000008">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 4"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000085">
        <property type="entry name" value="low-density lipoprotein receptor-related protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000098">
        <property type="entry name" value="low-density lipoprotein receptor-related protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000006">
        <property type="entry name" value="Putative low-density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722:SF15">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3A0</accession>
    <name>A0A3B5M3A0_9TELE</name>
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            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    <proteinExistence type="predicted"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1085" mass="124463" checksum="FFCC429950460E86" modified="2018-12-05" version="1">MAAQSRSPPLLLLVSTLCLWSSVTVLGLKVANSPAETPKQSDVRAVDHPDRNAPVAAAAGASQPRRASGWKLAEEAACREDLTRLCPKQTWSNNLAVLECLQDRREEIELAPDCNHLLWNYKLNLTTDPKFESVATEVCRSTIAEIKECNEEERGRGYLVSCLVDHRGNISEYQCNQYITKITSIIFSDYRLICGFMDKCKEDINSLRCGSINVGQKDTHSQGEVISCLERALVRQAELQERVHPIKEECQKAILRVAELSSDDFHLDRHLYFACRDDRERFCQNVQAGEGKVYKCLFNHKFEEAMSEKCRDALTTRQKLISQDYRVSYSLAKACKLDLRKQRCSLDSSLPRAREARLSYLLLCLEAAVHRGRPVSGECQGEMLDFRRMLMEDFSLSPEIVLHCRTEIEAQCSGLHRKGRTLHCLMRLGRGDLLLQLQTLIQSADPGADYRIDRALNEACESVIQTACKHIRSGDPMILSCLMEHLYTEKMVGDCEHRLLELQYFISRDWKLDPILYRKCQGDAARLCHTLGWNETSELMPPGAVFSCLYRHAYRTEEQGRFPSSQQFAGVQLSRDCKVEVQRILHQRALDVKLDPELQKRCMTDLGKWCSDKTDTGQELECLQEHLEDLVSSCREVVGNLTELESEDIQIDALLIRACEPVIQAHCHDVADNQIDTGDLMECLVQNKHQKEMNEKCAVGVTHFQLIQMKDFRFSYKFKMACKEDVLRLCPNIKKKVDVVICLSTTVRNDTLQEVREQRVSLKCRKQLRVEELEMSEDIRLEPELYEPCKSDISRLCPNVAFGNAQMIECLKEQKKQLSQRCHQRIFKLQEVEMSDPELDYQLMRVCKQMIKRFCTEADARNVLQCLKQNKNSELMDPKCKQMITKRQITQNTDYRLNPVLRKACRADIPKFCQSILNKASEDSELEGQIIACLKLKYADQISRLCAEEAAAQEQTGQVEECLKGNLLKIKQEACKKVNHHPRRTYLLTRFSTRPMSCLMEALQDKRIRLQPECKKRLQDRIDMWSYAAKVAPAEGFSDLAVQVMTSPSKNYILLVMALSVCVLFLVGLLCGRITKRVTRELKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3C0</accession>
    <name>A0A3B5M3C0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="12">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPP phosphatase family. PP-1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3C0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018648"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018883.1">
        <property type="protein sequence ID" value="ENSXCOP00000018648.1"/>
        <property type="gene ID" value="ENSXCOG00000014039.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154644"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000144">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF510">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="14">
        <location>
            <begin position="121"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="258"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="274"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="283" mass="32302" checksum="A731A97EB211DB91" modified="2018-12-05" version="1">KMAEGELNVDSLISRLLEVRGCRPGKIVQMTEAEVRGLCIKSREIFLSQPILLELEAPLKICGDIHGQYTDLLRLFEYGGFPPEANYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRFNIKLWKTFTDCFNCLPIAAIIDEKIFCCHGGLSPDLQSMEQIRRIMRPTDVPDTGLLCDLLWSDPDKDVQGWGENDRGVSFTFGADVVSKFLNRHDLDLICRAHQILKPSEKKAKYQYGGVNSGRPVTPPRTTQAPKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3D3</accession>
    <name>A0A3B5M3D3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fibrinogen C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3D3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018663"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018898.1">
        <property type="protein sequence ID" value="ENSXCOP00000018663.1"/>
        <property type="gene ID" value="ENSXCOG00000014048.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160129"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.530.10:FF:000001">
        <property type="entry name" value="angiopoietin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000001">
        <property type="entry name" value="Tenascin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.530.10">
        <property type="entry name" value="Gamma-fibrinogen Carboxyl Terminal Fragment, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057439">
        <property type="entry name" value="ANG-1/2/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037579">
        <property type="entry name" value="FIB_ANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020837">
        <property type="entry name" value="Fibrinogen_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040941">
        <property type="entry name" value="GGGWT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF6">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25443">
        <property type="entry name" value="ANG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00514">
        <property type="entry name" value="FIBRINOGEN_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="8">
        <location>
            <begin position="96"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="318" mass="36409" checksum="A4BC6576F16A6A86" modified="2018-12-05" version="1">MFSVKGFFSASFLCVSFSHLESKVQLLESQQRGELEDMRLEKNRLQVKTQMVAIESLERQLRVATSNNTALQKQQAQLMESVHTLIDMVSTTTGSPPRSQMWKDCADAYKAGHSASGLYLIYIGNRTEPVQVYCDMETSGGGWTVFQRRFNGSVDFQRSWREYKMGFGDVSGEHWLGNEVLYLLTSQGQYSLRVELQDWEGISAHSQYDRFTMASERRQYRLYLRGYSGTAGRQSSLITHGAGFSTRDHDNDNCDHCKCALMLTGGWWFDACGFSNLNGIYYTVGHNIRKLNGIKWHHFRGPSYSLRSTSMMVRPYDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3G8</accession>
    <name>A0A3B5M3G8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ubiquitin-conjugating enzyme E2 H</fullName>
            <ecNumber evidence="9">2.3.2.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">(E3-independent) E2 ubiquitin-conjugating enzyme H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">E2 ubiquitin-conjugating enzyme H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Ubiquitin carrier protein H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Ubiquitin-protein ligase H</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N(6)-monoubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with MAEA and WDR26, components of the CTLH complex that contains GID4, RANBP9 and/or RANBP10, MKLN1, MAEA, RMND5A (or alternatively its paralog RMND5B), GID8, ARMC8, WDR26 and YPEL5.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.24" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3G8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018007"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018234.1">
        <property type="protein sequence ID" value="ENSXCOP00000018007.1"/>
        <property type="gene ID" value="ENSXCOG00000013567.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004852"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000078">
        <property type="entry name" value="ubiquitin-conjugating enzyme E2 H isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000079">
        <property type="entry name" value="ubiquitin-conjugating enzyme E2 H isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="18">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="121"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00076312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00078369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00082119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="152" mass="17204" checksum="C252A348099D31F0" modified="2018-12-05" version="1">MSSPSPGKRRMDTDVVKLIESKHEVTILSGLNEFVVKFYGPQGTPYEGGVWKVRVDLPDKYPFKSPSIDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSEDEAQDMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3M6</accession>
    <name>A0A3B5M3M6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Synaptotagmin-7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Synaptotagmin VII</fullName>
        </alternativeName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="24">Homodimer. Can also form heterodimers with SYT6, SYT9 and SYT10. Interacts with calmodulin (CALM1, CALM2 or CALM3). Interacts with CD63; required for localization to lysosomes. Interacts with APP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="23">Cytoplasmic vesicle</location>
            <location evidence="23">Phagosome membrane</location>
            <topology evidence="23">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Synaptic vesicle membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Peroxisome membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Presynaptic cell membrane</location>
            <topology evidence="22">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3M6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014574"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014760.1">
        <property type="protein sequence ID" value="ENSXCOP00000014574.1"/>
        <property type="gene ID" value="ENSXCOG00000011059.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157180"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071912">
        <property type="term" value="P:asynchronous neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990927">
        <property type="term" value="P:calcium ion regulated lysosome exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001778">
        <property type="term" value="P:plasma membrane repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051050">
        <property type="term" value="P:positive regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017158">
        <property type="term" value="P:regulation of calcium ion-dependent exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030100">
        <property type="term" value="P:regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050796">
        <property type="term" value="P:regulation of insulin secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08386">
        <property type="entry name" value="C2A_Synaptotagmin-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08405">
        <property type="entry name" value="C2B_Synaptotagmin-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000027">
        <property type="entry name" value="Synaptotagmin 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000028">
        <property type="entry name" value="Synaptotagmin 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037732">
        <property type="entry name" value="C2A_Synaptotagmin-7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037741">
        <property type="entry name" value="C2B_Synaptotagmin-7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF375">
        <property type="entry name" value="SYNAPTOTAGMIN VIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
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        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19" id="KW-0966">Cell projection</keyword>
    <keyword evidence="21" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="18" id="KW-0458">Lysosome</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0564">Palmitate</keyword>
    <keyword evidence="17" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0770">Synapse</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="C2" evidence="28">
        <location>
            <begin position="267"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="28">
        <location>
            <begin position="398"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="109"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="207"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="24"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="27">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="27">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="211"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00046300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00060464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00064595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00067957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00080491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="535" mass="59725" checksum="14DEF0C322BB2AB6" modified="2018-12-05" version="1">MQLYKSFGKRNKPGMEAASSPESVRGRGENKAINDLDRDFWNNNDSSNVQQRWSSYPPKEFLLNMSPYAPYGDPRLTPNFGDASLSSASTLEHIPSSAVARPRPLVRQQSLQQPLTHQPPPGPNDPPVTSQSLGQLHTGPGGGGHRGGPRGVRGSPAGASRYRGTGAGRSRSNPGSWDHMMEQIRHRGLDVKSFLICLFCVHFTRTPGNKPGSSPRGQTPDETGRRQGQNHEANSTVTDLANSVTSDVLMLSPGSEEDEHEGPVCEKLGRIQFSVGYSFQDSTLTVKILKGQDLPAKDFSGTSDPFVKIYLLPDKKHKLETKVKRKNLNPHWNETFLFEGFPYEKVVQRTLYLQVLDYDRFSRNDPIGEVSIPLNKLDLANMQTFWKELKPCSDGSGSRGDLLVSLCYNPTANTITVSIIKARNLKAMDIGGTSDPYVKVWLMHKDKRVEKKKTVVMKRCLNPVFNESFPFDVPAHVLRETTIIITVMDKDRLSRNDVIGKIYLSWKSGPAEVKHWKDMISHPRNTVAQWHSLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3U7</accession>
    <name>A0A3B5M3U7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSXCOT00000018262.1">
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        <property type="gene ID" value="ENSXCOG00000013587.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00470000042444"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048599">
        <property type="term" value="P:oocyte development"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="P:spermatogenesis"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
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        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
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    </dbReference>
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        <property type="entry name" value="GATA-type_Znf_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
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        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47341">
        <property type="entry name" value="GATA-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47341:SF1">
        <property type="entry name" value="GATA-TYPE ZINC FINGER PROTEIN 1"/>
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    </dbReference>
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        <property type="entry name" value="GATAZNFINGER"/>
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        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="4">
        <location>
            <begin position="268"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="196"/>
            <end position="222"/>
        </location>
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        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="345" mass="38692" checksum="AA40D96ACEAF0F1F" modified="2018-12-05" version="1">MGSSEEVVEDSGEGCSLQIYSAEKTSAHAVALCECSSAVTLQSLQSYREKPSVGKQQSLIQECKKDWFLTEKDHLNVLFPENALQQAQTSSVCSTAQSTFSSTYMECTDVLTLDHNANVTLSTEKLCNTQLPPTSAGLLSPQLGQYDQFSASKTECCHVPQEESHPVADYSTVSSCSLSYATDIWKVSNDEKDQLSTNKCRTKTRRKQPHPSRSADSQDPDFQGVTFRIDTELDDNRQQCRLLITSKYRWKWLRRILMITFLNCNSVLSEGKVCASCCTRKTPMWRDAEDGTPLCNACGIRYKKYRVRCVKCWHIPRKEGNSSSLCIRCGNLVKLTSAHGWPQTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAJ3</accession>
    <name>A0A3B5MAJ3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibroblast growth factor</fullName>
            <shortName evidence="2">FGF</shortName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAJ3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018291"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018522.1">
        <property type="protein sequence ID" value="ENSXCOP00000018291.1"/>
        <property type="gene ID" value="ENSXCOG00000013775.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157821"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="68" mass="7937" checksum="DBD13A33D065ACBF" modified="2018-12-05" version="1">MNSRGRLYGTRLFKDECKFKETLLPNNYNAYESFVYKGFYIGLSKHGRVKRGNKATTAMTVTHFLPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MB32</accession>
    <name>A0A3B5MB32_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UPAR/Ly6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MB32"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021715.1">
        <property type="protein sequence ID" value="ENSXCOP00000021453.1"/>
        <property type="gene ID" value="ENSXCOG00000016059.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163304"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050918">
        <property type="entry name" value="CNF-like_PLA2_Inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016054">
        <property type="entry name" value="LY6_UPA_recep-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035076">
        <property type="entry name" value="Toxin/TOLIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20914:SF9">
        <property type="entry name" value="COILED, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20914">
        <property type="entry name" value="LY6/PLAUR DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00087">
        <property type="entry name" value="Toxin_TOLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00021">
        <property type="entry name" value="UPAR_LY6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00134">
        <property type="entry name" value="LU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="9">
        <location>
            <begin position="65"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="173" mass="19088" checksum="E91E090A0F69542A" modified="2018-12-05" version="1">LGREFYGAKIERNSKGCIISELCISYSVNFGAERIVQNTKCCSEDLCNNNYLCILEYNSTTNGKKCFSCDGENCMKTLNCAGDENYCIKVTAGVSLTLKGCASESVCSDRAASLVNQITGSNISCCQSDYCNGASSTSINYHLFALPLFYNIIHFIYLCIYILIMCFECLEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MB99</accession>
    <name>A0A3B5MB99_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MB99"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021787.1">
        <property type="protein sequence ID" value="ENSXCOP00000021523.1"/>
        <property type="gene ID" value="ENSXCOG00000016116.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164390"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF140">
        <property type="entry name" value="UDP-GLUCURONOSYLTRANSFERASE 2A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="131" mass="15023" checksum="AE85BDFED47B1FEA" modified="2018-12-05" version="1">MKKLKDGNYDVLFADPIYPGYDLTAEILGIPLVLSLRFSLAHYWERMCGQLPAPPSFVPAAMSKLTDKMDFSERLWNVLFYALQDVMLGKADIWLLRTYWDFDFPRPLLPNFKFVGGIHCKPAKPLPKVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MC75</accession>
    <name>A0A3B5MC75_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SH2 domain containing 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MC75"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021399.1">
        <property type="protein sequence ID" value="ENSXCOP00000021140.1"/>
        <property type="gene ID" value="ENSXCOG00000015815.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155314"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13157">
        <property type="entry name" value="PTB_tensin-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00173">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15832:SF3">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15832">
        <property type="entry name" value="SHC (SRC HOMOLOGY DOMAIN C-TERMINAL) ADAPTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="336" mass="38039" checksum="7F2FCA8C731C4562" modified="2018-12-05" version="1">YVGSFLVDDCCLDDQIKQLHTQLKYLKQACKKIRPVTLKFSLKGVKIYDEDEMTLLMAHALRRVSLSTARPIDGQFAFVSHNPGSTYGQLYCHVFKARHARAAQFLNLLLCRCFQLFYLEKHPEEAQESSSGSLPQRNPSLLNHGFPLSVSALVSFRRAPFRGLRLSTKSSPSLTRKKVIRAKGLHSGAYRSFTFTPLKQRSVHEQLSVSQKGQDNVPVRRSRAPSLAETEEALAQAVWSWAGLADSSYSLLADDVMGSYLLCPHPKKPKSGSLIVRFPSGLKTHLIENTRKGKFMLEKCRREFHSLGELIEYYSVTPDELPCQLCCARVNHCYEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MC80</accession>
    <name>A0A3B5MC80_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L17</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MC80"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021853"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022117.1">
        <property type="protein sequence ID" value="ENSXCOP00000021853.1"/>
        <property type="gene ID" value="ENSXCOG00000016326.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183010"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.10:FF:000003">
        <property type="entry name" value="60S ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_uL22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_uL22_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593:SF10">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="166" mass="19157" checksum="886767F2F78EA9DC" modified="2018-12-05" version="1">MDLGSGFCSNTRETAQAIKGMHIRKANKYLRDVIVKHQCVPFRRYNGGVGRCAQAKQFGWTQGRWPKKSAEFLLHMLKNAESNAELKGLDVDSLVIEHIQVNKAPKMRRRTYRAHGRINPYMSSPCHIEMILTEKEQIVPKPEEEVAQKKKVSQKKLKKQKLMARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCN6</accession>
    <name>A0A3B5MCN6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Alpha glucosidase 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCN6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021305"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021565.1">
        <property type="protein sequence ID" value="ENSXCOP00000021305.1"/>
        <property type="gene ID" value="ENSXCOG00000015943.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164305"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004558">
        <property type="term" value="F:alpha-1,4-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06602">
        <property type="entry name" value="GH31_MGAM_SI_GAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00111">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000072">
        <property type="entry name" value="lysosomal alpha-glucosidase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000001">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1760:FF:000001">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.110.10">
        <property type="entry name" value="Spasmolytic Protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030458">
        <property type="entry name" value="Glyco_hydro_31_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030459">
        <property type="entry name" value="Glyco_hydro_31_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000519">
        <property type="entry name" value="P_trefoil_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044913">
        <property type="entry name" value="P_trefoil_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF104">
        <property type="entry name" value="P-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00088">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00018">
        <property type="entry name" value="PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57492">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00129">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00707">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51448">
        <property type="entry name" value="P_TREFOIL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="P-type" evidence="10">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="862" mass="97090" checksum="84E2B06A297FC66C" modified="2018-12-05" version="1">PPAPGQALGPAWRFDCYPEKAVVVTRELCQARSCCFIPASGSTRGRSRKNGIPWCFYPPDFPSYSLVSSKETSMGLKGTLVKDVKTYYPGDILTLEMEIRHETDTRLRVRITDPSSSRFEVPLSVPTATKKAENTKYVVELSKQPFGLVVKRKSTGTVLLNTTVAPLFYADQFLQLSTALPSQFIYGLGEHRSTFLHDVQWNTLTMWARDVPPTEQTNLYGVHPFYLVMEDGGDAHGFFLLNSNAMDVALQPGPALTWRSIGGILDFYVFLGSDPGSVVQQYVEVIGQPAMPIYWALGYHLCRWGYKTSNATWEVVKRMRNYGIPQDVQWNDIDYMDQAKDFTFDPTNFNTLPDLVRDLHAHNQTYVMIMDPGISSTQPEGFYWPFDEGLKRGVFIKDAEGKTLIGKVWPGLTAFPDFSDEATHEWWYENLQRFHEKVPFDGLWIDMNEPSNFLDGSTVGCPSNSLENPPYTPGVLGGLLRSKTVCASAQQKLSTHYNLHSLYGLMEAKATSSALKRILAKRPFVISRSTFPSHGSYSGHWLGDNRSHWKDLYTSIAGMLTFNLLGIPLVGADVCGFSEEPQEELCVRWTQLGAFYPFARNHNSLDMNPQDPTAFSPSARTAMKQALLLRYSFFPFLYTLFHHAHVKGHTVARPLMFEFPKDVKTYGIDSQFLWGRSLLVTPVLEPGVDYVDGYFPEGLWYDYYTGDSVSSKGEKLRLSAPMDKINLHLRQGSIIPTQEPNVTLWISSGQPLHLVSALSDDGSASGDLFWDDGETIDTYETDRYTYVVFNVSQNTMTSSVLHSDQEGLFLSIDSVSFYGVKQKPSAVLVNSQEFPFIYRNNQVLSVASLSLRLTQNFTISWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDH1</accession>
    <name>A0A3B5MDH1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable ribosome biogenesis protein RLP24</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Ribosomal L24 domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the biogenesis of the 60S ribosomal subunit. Ensures the docking of GTPBP4/NOG1 to pre-60S particles.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Associated with nucleolar and cytoplasmic pre-60S particles. At the end of biogenesis it dissociates from cytoplasmic pre-60S particles and is likely to be exchanged for its ribosomal homolog, RPL24.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027901144.1">
        <property type="nucleotide sequence ID" value="XM_028045343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDH1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022313"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022586.1">
        <property type="protein sequence ID" value="ENSXCOP00000022313.1"/>
        <property type="gene ID" value="ENSXCOG00000016532.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114161767"/>
    <dbReference type="KEGG" id="xco:114161767"/>
    <dbReference type="CTD" id="51187"/>
    <dbReference type="GeneTree" id="ENSGT00950000183105"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000001">
        <property type="entry name" value="probable ribosome biogenesis protein RLP24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023442">
        <property type="entry name" value="Ribosomal_eL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF8">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN RLP24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01073">
        <property type="entry name" value="RIBOSOMAL_L24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="TRASH" evidence="9">
        <location>
            <begin position="6"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="164" mass="19548" checksum="665B4FAF7B13771A" modified="2018-12-05" version="1">MRIEKCYFCSGPVYPGHGVMFVRNDCKMFRFCKSKCHKNFKKKRNPRKTRWTKAFRKAAGKELTVDNSLEFEKRRNIPVKYNRQLWDKTVEAMKKVEEIKVKRQARFIMNRLKKGKQLEKEEAISEVKKNIHLIKAPHAGKAKQLEDKMVQRLQEDVEMGDEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDZ0</accession>
    <name>A0A3B5MDZ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Methionine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Methionyl-tRNA synthetase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Mitochondrial methionyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDZ0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022478"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022753.1">
        <property type="protein sequence ID" value="ENSXCOP00000022478.1"/>
        <property type="gene ID" value="ENSXCOG00000016798.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075136"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.220.10:FF:000001">
        <property type="entry name" value="methionine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000022">
        <property type="entry name" value="Methionyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023457">
        <property type="entry name" value="Met-tRNA_synth_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43326:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43326">
        <property type="entry name" value="METHIONYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 15" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Methionyl/Leucyl tRNA synthetase" evidence="16">
        <location>
            <begin position="34"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl-tRNA synthetase anticodon-binding" evidence="17">
        <location>
            <begin position="419"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF19303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="577" mass="66244" checksum="196278DF9E0FBE2D" modified="2018-12-05" version="1">MMQRGVLSADQRLWSSALKCSRSSSQLCKEKCFYITTPIFYVNAAPHLGHLYSAVIADCLHRWKLLQGFESRFATGTDEHGLKIQQAADAAGKDPLTFCTEVSQRFETLFSSCSVSFTDYIRTTEQRHRRAVEHFWSVLWHKGLIYKGSYEGWYSTQDESFLMPSQVGDALDPSGKEVKVSLESGHKVEWMKEENYMFRLSSFRSLLLDWLVKNPGAVQPERFHQAVLQWLQEDLPDLSVSRQRSRLQWGIPVPGDPEQTIYVWLDALVNYLTVVGYPEKHERWWHVAHHVIGKDILKFHAIYWPAFLLGAGLPLPQVIHVHSHWTVGGRKMSKSLGNVVDPFEHSQMFTNDGMRYFLLRQGVPDTDCDYTHDKVLKLLNAELADSLGGLLNRCTAPALNPAQVYPAFCSHSFPRDRRGRAVEEDYHMVETVRSLSTVVEKHYESMHVYKALEAISACVRQTNGFIQRHTPWKLDRRDSKDVRWLDTIIHLTLECLRVYGTLLQPVVPEISDRLLTRLGVQSQERSWKDLDFLGRYFGTDCSFEGRPLGSNCGLLFSRLETQSGDKQKTMKSKKPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5ME28</accession>
    <name>A0A3B5ME28_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha-2A adrenergic receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Alpha-2A adrenoreceptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5ME28"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019506"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019746.1">
        <property type="protein sequence ID" value="ENSXCOP00000019506.1"/>
        <property type="gene ID" value="ENSXCOG00000014656.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161451"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004938">
        <property type="term" value="F:alpha2-adrenergic receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051379">
        <property type="term" value="F:epinephrine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15322">
        <property type="entry name" value="7tmA_alpha2A_AR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002233">
        <property type="entry name" value="ADR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF24">
        <property type="entry name" value="ALPHA-2A ADRENERGIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01103">
        <property type="entry name" value="ADRENERGICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 15" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 15" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 15 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="32"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="204"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="244"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="263"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="367" mass="42246" checksum="3ADFDD1DDB1D7E18" modified="2018-12-05" version="1">MRRKNLGNLAPYSLRIALPLLVGFMILLIVFGNVLVVIAVFTSRALRAPQNLFLVSLASADILVATLVMPFSLANELMGYWYFGDVWCEIYLALDVLFCTASITHLCAISLDRYWSITQAIEYNLKRTPRRIKCIIFIVWVIAAVISFPPLITMEKNNNEAYPECKINDQKWYVISSCIGSFFLPCVIMVLVYVRIYQIAKKRTRAPPGDRKPNENEEAKGEINGVDLEDSSSSDHKVNNPCSIRKKTNKGKPRLSQIKPGDSNVQKQESNTKGSRWKGRQNREKRFTFVLAVVIGVFVICWFPFFFTYMLKTLCISCNVPATLFKFFFWFGYCNSALNPLIYTVFNNDFRRSFKKILCKRDTRRYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEJ1</accession>
    <name>A0A3B5MEJ1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Forkhead box L1</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEJ1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018074"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018303.1">
        <property type="protein sequence ID" value="ENSXCOP00000018074.1"/>
        <property type="gene ID" value="ENSXCOG00000013622.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162251"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20027">
        <property type="entry name" value="FH_FOXL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000016">
        <property type="entry name" value="Forkhead box protein I1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047514">
        <property type="entry name" value="FH_FOXL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF204">
        <property type="entry name" value="FORKHEAD BOX PROTEIN L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="47"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="47"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="138"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="310" mass="34179" checksum="E0AD514300966870" modified="2018-12-05" version="1">MALFPGRLPPLAVSSSALSLSGTPLIYLYGGDSTLSLVPGSQEPPQKPPYSYIALIAMAIRSAPGQRATLSGIYQFIMDRFPFYQDNKQGWQNSIRHNLSLNDCFIKLPREKGRPGKGSYWTLDSSCLDMFENGNYRRRKRRAKSQQDAPDSKDPGSRRAKVAVSSRETLNSDWGTAPVPDKEPNTEPVLLKFTQQNPVAQRQKAHISTEPHADPGSAQRANLLAAAQQTPRANEQRTDRSKGFTIESILSKNQDEARGRVLDVSAESLCAPPLVSAHPHHLVQVGLPFCSYLSLTCSDSALLTNKLFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFI4</accession>
    <name>A0A3B5MFI4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sodium/calcium exchanger membrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Ca(2+)(out) + K(+)(out) + 4 Na(+)(in) = Ca(2+)(in) + K(+)(in) + 4 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:69967"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MFI4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018596.1">
        <property type="protein sequence ID" value="ENSXCOP00000018364.1"/>
        <property type="gene ID" value="ENSXCOG00000013830.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234532"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000037">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000005">
        <property type="entry name" value="sodium/potassium/calcium exchanger 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00367">
        <property type="entry name" value="calcium/sodium antiporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF42">
        <property type="entry name" value="SODIUM_POTASSIUM_CALCIUM EXCHANGER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="388"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="494"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="526"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="11">
        <location>
            <begin position="41"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="11">
        <location>
            <begin position="390"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="312"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="312"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="323"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="557" mass="61838" checksum="9F81FE0F47289572" modified="2018-12-05" version="1">LSLFPDVYTFFCFCVIAAIHEFPRDYFTNQERTEGAVGLHVLCAVYMFYALALVCDDYFVPSLEKICERLQLSEDVAGATFMAAGSSAPELFTSVIGVFITKGDVGVGTIVGSAVFNILCIIGVCGIFALQTIRLSRWPLLRDSVYYTLSISALIVVSADGILYQSTSLLDGKILLSNIINHISDSFPLRSDYQSPHHLNGVNIFSVSHAAAVVCVRLLTANHHSKPSVLMVDELLSAYPHQLTFSEAGMRIMITSHFSPRTRLTMASRMLITERQRLINTRTLTNGESDVSVKGGGRRGIENGLSRAERRLSIRRDDREAGNETENEDNENNENDEEEEEDERDGPFVPFQCPAGVFNKLKWLLAWPLCLVLFFTVPNCSKPRWENWFMLSFFFSTLWIASFSYIMVWMVTVIGFTLGIPDVIMGITFLAAGTSVPDCMASLIVARQGMGDMAVSNSIGSNVFDILVGLGLPWALKTLAINYGSAIKLNSKGLIFSVGLLLASVFLTVLGVHLNKWTLDKRLGLTCLLFYSIFLCFSCLIEYNIFTFVNLPTCRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGG6</accession>
    <name>A0A3B5MGG6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transforming acidic coiled-coil-containing protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TACC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGG6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022705"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022981.1">
        <property type="protein sequence ID" value="ENSXCOP00000022705.1"/>
        <property type="gene ID" value="ENSXCOG00000016964.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156991"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021987">
        <property type="term" value="P:cerebral cortex development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1700:FF:000001">
        <property type="entry name" value="Transforming acidic coiled-coil-containing protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039915">
        <property type="entry name" value="TACC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007707">
        <property type="entry name" value="TACC_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13924">
        <property type="entry name" value="TRANSFORMING ACIDIC COILED-COIL CONTAINING PROTEIN 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13924:SF12">
        <property type="entry name" value="TRANSFORMING ACIDIC COILED-COIL-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05010">
        <property type="entry name" value="TACC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transforming acidic coiled-coil-containing protein C-terminal" evidence="8">
        <location>
            <begin position="92"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="209"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="298" mass="34154" checksum="784B74113B40B45B" modified="2018-12-05" version="1">MSWLSPVQWAKWTWSAVTGGAGDNGQHTGRDDKEDNLMLFKDDHHEGHATDEEKLAGTKSEKEQNYTCELKTSEGSEMQKTGSQVLDSICISEAEKQAVLTLIREEIITKEAETNDWKKKYEQCKQEVDEMRKIVAEYEKTIAQMIEDEQRNSVNFQKALHAVTVEKDAALADLNSVERSLSDIFRRYENMKSTLDGFKKNEEVLKKCAQDYLARVKQEEQRYQTLKLHAEEKLDKANEEIAQVRTKASSETIALTASLRKGQMRNESLEQALLQKNQEIEELTKICDELIAKMGKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGR1</accession>
    <name>A0A3B5MGR1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGR1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022800"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023076.1">
        <property type="protein sequence ID" value="ENSXCOP00000022800.1"/>
        <property type="gene ID" value="ENSXCOG00000017037.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158008"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000004">
        <property type="entry name" value="collagen alpha-6(VI) chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000041">
        <property type="entry name" value="matrilin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036337">
        <property type="entry name" value="Matrilin_CC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF87">
        <property type="entry name" value="COLLAGEN ALPHA-1(VI) CHAIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="VWFA" evidence="9">
        <location>
            <begin position="89"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="327" mass="36490" checksum="374AC7AB9FE6A1DB" modified="2018-12-05" version="1">MNKQVHCFTAASVTHVKLPGVDLCQTLDHGCEHLCVSTTDSYICKCNEGFLLAENGKSCGSKQTKKFYINTFFMSLHLTEPGCHDGVMDLVFVIDGSKSLGPANFELVKQFVNSIVDSLDVSRTATRVGLIQFSTKVRTEFSLGQHAAAQDVKRAVSQMQYMGRGSMTGSALRHMFQFSFSEKEGARLNVPRVCVVFTDGRSQDDVSEWASKAKTLGVTIYALGVGKAIEQELREIASEPAEMHLYYAEDFEKMGEITKKLKSRMCKGKKKKHVLTVFTFAPTDKPADESLCRCESVIMFQSQVTEKLKNLAQSNILLFCWFPPQRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIK9</accession>
    <name>A0A3B5MIK9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">exo-alpha-sialidase</fullName>
            <ecNumber evidence="3">3.2.1.18</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of alpha-(2-&gt;3)-, alpha-(2-&gt;6)-, alpha-(2-&gt;8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.</text>
            <dbReference type="EC" id="3.2.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 33 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.18" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIK9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020951"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021209.1">
        <property type="protein sequence ID" value="ENSXCOP00000020951.1"/>
        <property type="gene ID" value="ENSXCOG00000015684.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182944"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004308">
        <property type="term" value="F:exo-alpha-sialidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006689">
        <property type="term" value="P:ganglioside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009313">
        <property type="term" value="P:oligosaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15482">
        <property type="entry name" value="Sialidase_non-viral"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011040">
        <property type="entry name" value="Sialidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026856">
        <property type="entry name" value="Sialidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036278">
        <property type="entry name" value="Sialidase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10628">
        <property type="entry name" value="SIALIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10628:SF23">
        <property type="entry name" value="SIALIDASE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13088">
        <property type="entry name" value="BNR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50939">
        <property type="entry name" value="Sialidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sialidase" evidence="8">
        <location>
            <begin position="70"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="222"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="246"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="268"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="405" mass="45734" checksum="106EC6D3EFBD476F" modified="2018-12-05" version="1">MGKTSSKSDEQTIEKQIVFKSKRGKVYKIPAIYYNENDKRLFAFAEKTQPKTLNKYATLSSKSYVIDLFQWSRPTEVVRKCSSGYRPMNPCVVYENHTQTLFLFFIYVIETELWQIEHHQNKARLCYITKKKNEEKWSEFTELTDLVPVFNNWSTFAIGPGHGIQTKTGRMIVPAYAYTKDKEPSPHAFCFYSDDYGSTWKCEEMLSETSMECQMTEILDSSSKDTHCQGRTEASASRRQPHHAAQSRTGQVYPSYQQDGQFRHRHQAQSTPISSYSGVQSQHGGFGTDGTRRTNPRTITAEVFHPGCSGGSCPTTVSHRGTDDDKGECKGIGCKLPHRASQKQQQQQQCVGSGCRTHAGDDGRRDSSPVQATDRAAQFLGELPDFGLDRGAWIQLACDMMPGEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLN4</accession>
    <name>A0A3B5MLN4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 16">Amyloid-beta A4 protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Functional neuronal receptor which couples to intracellular signaling pathway through the GTP-binding protein G(O).</text>
    </comment>
    <comment type="function">
        <text evidence="16">Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cell membrane</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15 16">Belongs to the APP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLN4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025314"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025621.1">
        <property type="protein sequence ID" value="ENSXCOP00000025314.1"/>
        <property type="gene ID" value="ENSXCOG00000018898.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063252"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005798">
        <property type="term" value="C:Golgi-associated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099503">
        <property type="term" value="C:secretory vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030546">
        <property type="term" value="F:signaling receptor activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051246">
        <property type="term" value="P:regulation of protein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.140:FF:000001">
        <property type="entry name" value="Amyloid beta (A4) protein b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.570.10:FF:000001">
        <property type="entry name" value="Amyloid beta A4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.770:FF:000001">
        <property type="entry name" value="Amyloid beta A4 protein-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.770">
        <property type="entry name" value="Amyloid precursor protein, E2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.140">
        <property type="entry name" value="Amyloidogenic glycoprotein, copper-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.570.10">
        <property type="entry name" value="Amyloidogenic glycoprotein, heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036669">
        <property type="entry name" value="Amyloid_Cu-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008155">
        <property type="entry name" value="Amyloid_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013803">
        <property type="entry name" value="Amyloid_glyco_Abeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011178">
        <property type="entry name" value="Amyloid_glyco_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024329">
        <property type="entry name" value="Amyloid_glyco_E2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008154">
        <property type="entry name" value="Amyloid_glyco_extra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015849">
        <property type="entry name" value="Amyloid_glyco_heparin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036454">
        <property type="entry name" value="Amyloid_glyco_heparin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019745">
        <property type="entry name" value="Amyloid_glyco_intracell_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019543">
        <property type="entry name" value="APP_amyloid_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019744">
        <property type="entry name" value="APP_CUBD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036176">
        <property type="entry name" value="E2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103">
        <property type="entry name" value="ALZHEIMER'S DISEASE BETA-AMYLOID RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103:SF7">
        <property type="entry name" value="AMYLOID-BETA PRECURSOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10515">
        <property type="entry name" value="APP_amyloid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12924">
        <property type="entry name" value="APP_Cu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12925">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02177">
        <property type="entry name" value="APP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03494">
        <property type="entry name" value="Beta-APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00203">
        <property type="entry name" value="AMYLOIDA4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00204">
        <property type="entry name" value="BETAAMYLOID"/>
    </dbReference>
    <dbReference type="SMART" id="SM00006">
        <property type="entry name" value="A4_EXTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56491">
        <property type="entry name" value="A heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89811">
        <property type="entry name" value="Amyloid beta a4 protein copper binding domain (domain 2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109843">
        <property type="entry name" value="CAPPD, an extracellular domain of amyloid beta A4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00319">
        <property type="entry name" value="APP_CUBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51869">
        <property type="entry name" value="APP_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51870">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00320">
        <property type="entry name" value="APP_INTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16" id="KW-0034">Amyloid</keyword>
    <keyword evidence="16" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0186">Copper</keyword>
    <keyword evidence="12 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="572"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="E1" evidence="19">
        <location>
            <begin position="23"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="E2" evidence="20">
        <location>
            <begin position="302"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="GFLD subdomain" evidence="15">
        <location>
            <begin position="23"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="CuBD subdomain" evidence="15">
        <location>
            <begin position="126"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="209"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="327"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="227"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="68"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="93"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="128"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="139"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="153"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00053261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="641" mass="73047" checksum="192F61F01F643FA6" modified="2018-12-05" version="1">VLPGFFTAAVFLIQVPMDDSVGLLTEPQVAMFCGKLNMHINVQTGKWESDPSGTKSCIGTKEGILQYCQEVYPDLQITNVVEANQPISIPNWCKKGRKQCRSHTHIVVPYRCLVGEFVSDALLVPDKCKFLHQEQMNKCESHLHWHTVAKESCGDRSMNLHDYGMLLPCGIDRFRGVEFVCCPAEEERKSDSAEQNEEESDVWWGAAENEYSDNSMTRPVDTQPAAAEDDEDDDEEEEAFERDEDGDGDEDDEEDDDDDVIDEQDSDERSASVAMTTTTTTTTESVEEVVRVPTIAPSPPDAVDQYLESPGDDNEHTDFRKAKESLEAKHREKMSQVMREWEEAERQVKNLPRSDKKAVIQHFQEKVEALEQEAAEERQQLVETHMARVEALLNSRRRLSLENYLSALQANPPRSRQVLSLLKKYVRAEQKDRQHTLKHYEHVRTVDPKKAAQIRPQVLTHLRVIDERMNQSLGLLYKVPSVASEIQKQVGNYRKFSLVLFNIVFLFLSAVEPVDARPNPDRGLPTRPVSALKPEEMPEVRMDTEERQSARYEVYHQKLVFFAEDLGSNKGAIIGLMVGGVVIATVIVITLVMLRKKQYTSIHHGVIEVDAAVTPEERHLAKMQQNGYENPTYKFFEQMQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MMR4</accession>
    <name>A0A3B5MMR4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox protein Hox-D3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MMR4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025714"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026023.1">
        <property type="protein sequence ID" value="ENSXCOP00000025714.1"/>
        <property type="gene ID" value="ENSXCOG00000019218.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160027"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000094">
        <property type="entry name" value="Homeobox protein Hox-A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025281">
        <property type="entry name" value="DUF4074"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664:SF5">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664">
        <property type="entry name" value="PROTEIN ZERKNUELLT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13293">
        <property type="entry name" value="DUF4074"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="174"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="176"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="148"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="233"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="379"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="72"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="390"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="411" mass="44692" checksum="F86A96BC38DD846D" modified="2018-12-05" version="1">MQKATYYDNSGLFGGYTYPKPDSYSYGPAHQSYPAPNIDTDYQGSVCPIQTSAVRPPPALKDSDLNGDCMRQSSSQSNNNNNNNSSSSSSSGSSQANSISEQQVPPLSAASPSSNSSTSQKKKSPNNSASGPATPALTKQIFPWMKETRQNSKQKSNNNYAAAEVSDEKSPPGPASKRVRTAYTSAQLVELEKEFHFNRYLCRPRRVEMANLLNLTERQIKIWFQNRRMKYKKDQKSKGLAHSPMGHSPDRSPPLSGPNHTGYAGQLQNVNSLSYDAPSPPPFAKPQQNMYGLAAYTAPLGGCIPQQKRYPGSEYEHHSMQSNGGFANANLQGSPVYVGGNFVDSMPASGPMFNLGHLPHPSSTSVDYSCAAQIPGNHHHGPCDPHPTYTDLTSHQVSQGRIQEAPKLTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MMZ2</accession>
    <name>A0A3B5MMZ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VWFD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MMZ2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022521"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022796.1">
        <property type="protein sequence ID" value="ENSXCOP00000022521.1"/>
        <property type="gene ID" value="ENSXCOG00000016834.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164871"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050780">
        <property type="entry name" value="Mucin_vWF_Thrombospondin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339">
        <property type="entry name" value="EXTRACELLULAR MATRIX GLYCOPROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339:SF371">
        <property type="entry name" value="MUCIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00216">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017204715" description="VWFD domain-containing protein" evidence="3">
        <location>
            <begin position="19"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="VWFD" evidence="4">
        <location>
            <begin position="25"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="218" mass="24944" checksum="C36D86990192A2DC" modified="2018-12-05" version="1">MCVYIYIYIALTVSLTFIYDNHVNNICSTWGTQHFKTFDGDVFQFPGLCEYNLVKDCRESEKKFSVHIRREENDGNITIRYVVVTISENTFNLTETEVTENGNPVTLPHYSGGVKVEESTFYIRLRATKVGIIVMWNREDAVMVEVSNRYKNQTCGLCGDFNGVPVYNEFMDNGFKVTPIELGIKHRVHLPNDECKDPPVEDESKTNVSHSCKAFVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MN19</accession>
    <name>A0A3B5MN19_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein lifeguard 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Fas apoptotic inhibitory molecule 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">Interacts with FAS/TNFRSF6 and BAX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane raft</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Postsynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the BI1 family. LFG subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MN19"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025255.1">
        <property type="protein sequence ID" value="ENSXCOP00000024951.1"/>
        <property type="gene ID" value="ENSXCOG00000018628.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244890"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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    <sequence length="231" mass="26186" checksum="45DF32DF7D02617C" modified="2018-12-05" version="1">QVGNENEPPSYQEATAYPEMEAQFAWDDKTIRRTFIRKVYAILMLQLSVTVGIVCLFTFAPVRFYIQTNPSLYMASIMFFATYIALSCCGDLRRQFPWNITLLVLFTLSMAFMMGFVSFYNTKSVMLCLGITALVCLSVTVFSFQSKIDVTSCQGVLFSLCMAMLLCAITISIVVPFGYLLQFLAFDTQLLLGNKRYAISPEEYIFATLSLYLDIIYLFSFLLQLTGTGRD</sequence>
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</entry>
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    <sequence length="149" mass="15927" checksum="27126CEC37AB1DF7" modified="2018-12-05" version="1">MFQFSKYPMDILEMLSGHQAHQFKGLGLERQLSHQQVQLQHQQQLQQQHQPSADTSGSLLSGLGLGSLQSSRSNAFADSSSLFAKMSAPPPSISHQSQSSSSSHSSRKSSKISSSSSSGSGSSSSGYPQFLRPFHPAEAALAQEQLHSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MP91</accession>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="38"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="136"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="312"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="148"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="196"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="274"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="562" mass="64922" checksum="7C1972F813D22304" modified="2018-12-05" version="1">MNGADTQRRSCFLPVNCCVQTKGNETDALPSCLQPDLLNFKKGWMSKLDDCGEWKKHWFVLTDAGLKYYRDSTAEEAYDLDGEIDLKSCLKVSEFDVEKNYGFQIQTREAVFTLSAMTAGIRRNWIEVLKKSIRPSSSPDLTQLPDNNNKENNDKEDSHKRFPPSSRRPSSRHADSQPETPSSAHPAERRFDYVELSPVPASSSAPLPSSQRESGEGQGREHSQWQEERSVSSQWEAILSRKGSGVGSNQKPHLDDEIERKWYELERMPLKDPSSLSSMGSRTSSQSANEALQREVVVGGDCSTEAPCSRSLAAMERAHRHALEELQRQHDRQMRELETEKERLLLEETRDTARVMDALKKKHKEELEREVEKVRSFTFSHFFFFIFLISLTRRCRAETLALQRELAGLSERYSQKCLELNKAEQRNAEREREVSHRERDMEQLKKENMELKGRLTDEMSRTRSALTDRGSEDDRDKMSCELEVLLRMKDNEIEYLHKEISCLRNELQFLNMLACERYVELHGELNGMRGRTEREIQSLKEHLRLAMAALQEGQKLGNSLDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPU0</accession>
    <name>A0A3B5MPU0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation machinery-associated protein 16</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the biogenesis of the 60S ribosomal subunit in the nucleus.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Associates with pre-60S ribosomal particles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMA16 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MPU0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021494"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021757.1">
        <property type="protein sequence ID" value="ENSXCOP00000021494.1"/>
        <property type="gene ID" value="ENSXCOG00000016090.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004179"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.170:FF:000001">
        <property type="entry name" value="Translation machinery-associated 16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.170">
        <property type="entry name" value="Translation machinery-associated protein 16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021346">
        <property type="entry name" value="Tma16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038356">
        <property type="entry name" value="Tma16_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349:SF2">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11176">
        <property type="entry name" value="Tma16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="143" mass="16637" checksum="0215B050DAEE3A93" modified="2018-12-05" version="1">MKSKAADKVVHPFSRKAAYLNREDVRLKRKERMKSEKATRLSNIGEKLLWFQSQLDPNKTSYTKRDACQVVERYLHRFDSELEQIELVNGIKGRQGRLHGAREAAIKQSVERERAQFEGAGLEIPDIINTKHLKSFRSDFEAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQE9</accession>
    <name>A0A3B5MQE9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQE9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025746"/>
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        <property type="protein sequence ID" value="ENSXCOP00000025746.1"/>
        <property type="gene ID" value="ENSXCOG00000019242.1"/>
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    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="265" mass="30551" checksum="9DDE37AD5DD754A2" modified="2018-12-05" version="1">MKTGSQIRLLLWKNWTLRKRQKIRFLVEISWPVLLFIGLVWLRKANPLYQQHECHFPNKALPSAGILPWIQGIFCNANNPCFRYPTQGESPGIVSNYNNSVLAHFYVDIQELLLNETEVRQYGRLWREMASFSNFMDTLRNNPSAIAGRGLKIDDILKDDEILTAFLLRDAGLSESIVYQLVNAQLRLEQFAFGVPDLQLKDIACSQALLEHFIIFPSRMGLHGVRNAMCALSQQRLQRIEDILYANLDFFKIFRLVGGLLKINP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQZ8</accession>
    <name>A0A3B5MQZ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Plexin-A1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm. Acts as coreceptor of TREM2 for SEMA6D in dendritic cells and is involved in the generation of immune responses and skeletal homeostasis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts directly with NRP1 and NRP2. Interacts with PLXN1B. Interacts with FARP2, RND1 and KDR/VEGFR2. Binding of SEMA3A leads to dissociation of FARP2. Interacts with CRMP1, DPYSL2/CRMP2, DPYSL3/CRMP3 and DPYSL4/CRMP4. Interacts (via TIG domains) with TREM2; the interaction mediates SEMA6D binding and signaling through TYROBP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027857778.1">
        <property type="nucleotide sequence ID" value="XM_028001977.1"/>
    </dbReference>
    <dbReference type="STRING" id="32473.ENSXCOP00000023616"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023899.1">
        <property type="protein sequence ID" value="ENSXCOP00000023616.1"/>
        <property type="gene ID" value="ENSXCOG00000017643.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114135059"/>
    <dbReference type="GeneTree" id="ENSGT01050000244850"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002116">
        <property type="term" value="C:semaphorin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001763">
        <property type="term" value="P:morphogenesis of a branching structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00603">
        <property type="entry name" value="IPT_PCSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01180">
        <property type="entry name" value="IPT_plexin_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01179">
        <property type="entry name" value="IPT_plexin_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01181">
        <property type="entry name" value="IPT_plexin_repeat3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12790">
        <property type="entry name" value="RasGAP_plexin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000005">
        <property type="entry name" value="Plexin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000006">
        <property type="entry name" value="Plexin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000123">
        <property type="entry name" value="Plexin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000320">
        <property type="entry name" value="Plexin A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000006">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000071">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000018">
        <property type="entry name" value="Plexin A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000329">
        <property type="entry name" value="Plexin A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031148">
        <property type="entry name" value="Plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013548">
        <property type="entry name" value="Plexin_cytoplasmic_RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046800">
        <property type="entry name" value="Plexin_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041019">
        <property type="entry name" value="TIG1_plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041362">
        <property type="entry name" value="TIG2_plexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625">
        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22625:SF35">
        <property type="entry name" value="PLEXIN-A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08337">
        <property type="entry name" value="Plexin_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20170">
        <property type="entry name" value="Plexin_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24479">
        <property type="entry name" value="PSI_PlexinA-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01833">
        <property type="entry name" value="TIG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18020">
        <property type="entry name" value="TIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17960">
        <property type="entry name" value="TIG_plexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 19" id="KW-0732">Signal</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017378461" description="Plexin-A1" evidence="19">
        <location>
            <begin position="34"/>
            <end position="1891"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1238"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="20">
        <location>
            <begin position="22"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="1259"/>
            <end position="1308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1891" mass="211779" checksum="C7D9BC77AB97792C" modified="2018-12-05" version="1">MCPLSRTLAFPGQGNLLFLLGVLLLMVSSLSGGQPPPFKRFAPADHFLTHLAIHNKTGEVYVGAVNWIFKLSSNLTKLRSHMTGPVVDNEKCYPPPSVQSCPHDLAQTPNVNKLLLIDYPQNRLIACGSTSQGICQFLRLDDLFKLGEPHHRKEHYLSSVAESGTMSGVIISSPQSPTSKLFIGTPIDGKSEYFPTLSSRKLMENEENADMFSFVYQDEFVSSQLKIPSDTLSKFPAFDIYYVYSFSSEQFVYYLTMQLDTKLTSPDASGEQFFTSKIVRLCMDDPKFYSYVEFPIGCTKDGIEYRLVQDAFLARPGRQLANSLGISENEDILFTVFSQGQKNRAKPPKESALCLFTLRKIKEKIKERIQSCYRGEGKLSLPWLLNKELDCINSPLQIDDNFCGQDFNQPLGGTSTIEGTPLFIDKDDGMTSVAAYDYRGNTVAFVGTRNGKLKKILVNSVSSNRPTVLYEKVTVSEGGSPLQRDMLFSPDLQHIYTLTDRQVVRVPVESCEQYTTCGECLGSRDPHCGWCVLHNVCSRKDRCERASEPQRFASDQRQCVELTVQPRNISVTMSEVQLVLQARNVPDLSAGVNCSFEDYVETEGRIQGGHIFCLSPSARDIAPITRNKGDKRVVKLFLKSKETGIKFASVDFVFYNCSVHQSCLSCVNGSFPCHWCKYRHMCTQNANDCSFQEGRVNDSEDCPQILPSTQIYMPAGVTKPITLAARNLPQPQSAQRNYECVFDIQGLTKSVPALRFNSTSIQCQNTEYTYEGDEISDLPVDLSVVWNGNFVIDNPYNIKAHLYKCFALRGSCGKCLKANPRFECGWCVGDKKCSLRQECTPPENTWMHATTGNSRCTHPKITKLYPETGPRQGGTMLTINGENLGLQFKDIIGGVRIGKVQCIPQEDQFISAEQIVCRLLDATGYRVQEAQVEVCVGEQPCINPDYKTVSSKAFTFVSPYFSRVHPSAGPLSGGTRITIEGSHLNAGSAVIVRIGLHQCTFERRSNKEIVCVTPAGQSPTPTPVMVDINSAELRNPEVKFNYTDDPTILKIEPDWSIASGGTMLTITGTNLATIKEPKMRAKYGAGQSENNCTVLNNTVMVCLAPSVAKSGKSFSEMGTSPDEIGFVMDGVRSVLVINETFSYYPDPEFESLGPKMELKPSSPLILKGRNLIPAAPGNSKLNYTVLIGEMPCVLTVSESQLLCESPNLTGEHKVTVRVGGFEASPGTLQIYSDSLLTLPAIIGIGIGGGLLLFVIIAVLIAYKRKSRDADRTLKRLQLQMDNLESRVALECKEAFAELQTDIHELTQELDGAGIPFLDYRTYAMRVLFPGIEDHPVLKEMEVQANVEKALTLFGQLLTKKHFLLTFIRTLEAQRSFSMRDRGNVASLIMTALQGEMEYATGVLKQLLSDLIDKNLESKNHPKLLLRRTESVAEKMLTNWFTFLLYKFLKECAGEPLFMLYCAMKQQMEKGPIDAITGEARYSLSEDKLIRQQIDYKTLTLHCVNPENENAPEVTVKTLNCDTVTQVKEKLLDAVYKGTPYSQRPKASDMDLEWRQGRMARIILQDEDVTTKIDNDWKRLNTLAHYQVTDGSVIALVPKQNSAYNISNSSTFTKSLSRYESMLRTASSPDSLRSRTPMITPDLESGTKLWHLVKNHDHSDQREGDRGSKMVSEIYLTRLLATKGTLQKFVDDLFETIFSTAHRGSALPLAIKYMFDFLDEQADKHSITDPDVRHTWKSNCLPLRFWVNVIKNPQFVFDIHKNSITDACLSVVAQTFMDSCSTSEHKLGKDSPSNKLLYAKDIPNYKNWVERYYSDISRMPVISDQDMSAYLAEQSRLHANQFNSMSALHEIYSYIVKYKDEILSALERDEQARRQRLRSKLEQVIDTMALAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MR01</accession>
    <name>A0A3B5MR01_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">D-isomer specific 2-hydroxyacid dehydrogenase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MR01"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026844"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027173.1">
        <property type="protein sequence ID" value="ENSXCOP00000026844.1"/>
        <property type="gene ID" value="ENSXCOG00000020045.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162740"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF257">
        <property type="entry name" value="GLYOXYLATE REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="3">
        <location>
            <begin position="17"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="139" mass="15100" checksum="D9B5E2649C6EA00D" modified="2018-12-05" version="1">MADNKPWVLISEVGDKGVHEDVVDIISQHFHIICLKEFLQNPALHGPKIQGLFMWYYSPAAEPSLMSCLPSLKVIANGGVGIDHLDVPYINSFGVKVTNTPGVVSDATADMAMALLLASARKVLEGESNTFPSYNYSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MR07</accession>
    <name>A0A3B5MR07_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Elastin microfibril interfacer 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MR07"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021914"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022181.1">
        <property type="protein sequence ID" value="ENSXCOP00000021914.1"/>
        <property type="gene ID" value="ENSXCOG00000016372.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234633"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011489">
        <property type="entry name" value="EMI_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF36">
        <property type="entry name" value="EMILIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07546">
        <property type="entry name" value="EMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51041">
        <property type="entry name" value="EMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="EMI" evidence="8">
        <location>
            <begin position="22"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="111"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="795"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="652"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="127"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="803"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="898" mass="100301" checksum="67EB7E32E2880FA2" modified="2018-12-05" version="1">SWLQPHLTSPKYCQLFSFIPTCLNWCAYVVQRNVSCVVQGNGASFQEPVVAPCPAYQPDCQQQVTYQTVFRPTYKIAIKTVTQLEWKCCPEYRGPNCKDLKPPTDRQTVQGALSLQPNPGYSPRHTQRAERRETPHYETQHGVTDKVRFLEREVQRLSQTVSDLQSTLTGLATNLRTDLQDDTKKMLETLLNNMRPPETSSNAEETPVVIDGHQATRGGIGGEKTLEKIEARLDDIDNALRNKDEALEDLRGISLSHEGQIRVLMDASQSQTPAIADFEGIQTYVDGKIEKLKKELDENVEEQMVKLQNLCNDKISAVQKTCEDSHDQVGVKLSKLLESKEADLRKEIQTIRLNMAASDGPVQTSRQTEPSEKKDYSDHKDIWREIDRIAEAHRILNVRFDNELGHLSSLQEIGDLGVLIQELEARVNITEQNAETHCFYIEDKLTRIIADEVEALRQLLDQRLITAEDQFTNILVEMSNNSFPRVSGDSTAALETEINNNKLLLKGLNQKVKAVEDLYSAGCPGSKIITDQGRIAFMPGRVENIVTDLNRYRNDLDVLRTDVSSNTQKLQQLENLFIRQPVGNERQVKTTEDFQKGLINLQDNVLALADAVTGLTDSMSKSKQDIQHISSTCCNAEVTGRGMPLQPNLASVDGTSRQIQELKNRLDTLNTQVSSELNNCRQNTDGISAIGGRVSRLENICGRLDGVSLNIRELKNGLEQHVGGLQNCVQRMNVTCGNHGAEITSLSNSLRKLQAQMSMITKHVLKDVAAKEPDRPSSLPDTSQRRAQIKQIHFPVIIPPPSSGSNQPTQGNKHAIRISKPNQPLNPQHPRLPTLPLVPVRPVVETGEAGPPGYIRRLNVRRGAEDSLSKPVQGFAGAPGELCWDRRSALRCMRQHAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MS89</accession>
    <name>A0A3B5MS89_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">NUBP iron-sulfur cluster assembly factor 1, cytosolic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NUBP1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Implicated in the regulation of centrosome duplication. Negatively regulates cilium formation and structure.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11">Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotetramer of 2 NUBP1 and 2 NUBP2 chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MS89"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024061"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024347.1">
        <property type="protein sequence ID" value="ENSXCOP00000024061.1"/>
        <property type="gene ID" value="ENSXCOG00000017966.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183193"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000427">
        <property type="entry name" value="Cytosolic Fe-S cluster assembly factor NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03038">
        <property type="entry name" value="NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028601">
        <property type="entry name" value="NUBP1/Nbp35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF35">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="58"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="66"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="315" mass="33627" checksum="54290F9B1AB0D6ED" modified="2018-12-05" version="1">MADVPENAPEHCPGTSSEEAGKSASCQGCPNQQLCASGATKAPDPAIEEIGEKLSVVKHKILVLSGKGGVGKSTFSAHLAHALASDSTKEVALLDVDICGPSIPRIMGLEGEQVHQSGSGWSPVYVDDNLAVMSIGFLLSSPDDAVIWRGPKKNGMIKQFLRDVDWGQLDYLVVDTPPGTSDEHLSIVQYLSSTNVDGAVIITTPQEVSLQDVRKEIRFCQKVKLPIIGVVENMSGFVCPKCKNTSHIFPPSSGGAEKMCADLNLPLLGKVPLDPRIGRSCDEGKSFLQEVPDSPAAEVYRRIVQSIQDYCSNAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSL8</accession>
    <name>A0A3B5MSL8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Kynureninase</fullName>
            <ecNumber evidence="10 11">3.7.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">L-kynurenine hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">KYNU</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 11">
            <text>3-hydroxy-L-kynurenine + H2O = 3-hydroxyanthranilate + L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25143"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36559"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58125"/>
            <dbReference type="EC" id="3.7.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10 11">
            <text>L-kynurenine + H2O = anthranilate + L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16813"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="3.7.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10 11">Amino-acid degradation; L-kynurenine degradation; L-alanine and anthranilate from L-kynurenine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10 11">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10 11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the kynureninase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.7.1.3" evidence="10 11"/>
    <dbReference type="RefSeq" id="XP_027865752.1">
        <property type="nucleotide sequence ID" value="XM_028009951.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027865753.1">
        <property type="nucleotide sequence ID" value="XM_028009952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSL8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022489"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022764.1">
        <property type="protein sequence ID" value="ENSXCOP00000022489.1"/>
        <property type="gene ID" value="ENSXCOG00000016808.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114140222"/>
    <dbReference type="GeneTree" id="ENSGT00390000008033"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00329"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00334">
        <property type="reaction ID" value="UER00455"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030429">
        <property type="term" value="F:kynureninase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097053">
        <property type="term" value="P:L-kynurenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000031">
        <property type="entry name" value="Kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000203">
        <property type="entry name" value="Kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01970">
        <property type="entry name" value="Kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010111">
        <property type="entry name" value="Kynureninase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01814">
        <property type="entry name" value="kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14084">
        <property type="entry name" value="KYNURENINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14084:SF0">
        <property type="entry name" value="KYNURENINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22580">
        <property type="entry name" value="KYNU_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038800">
        <property type="entry name" value="KYNU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 10 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10 11" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="5 10 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="165"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR038800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="464" mass="52039" checksum="FC4F2E5FF7DC6359" modified="2018-12-05" version="1">MDDIFDLSPTAAVKRVSALLDCSPTSEKVADYFDKHDKLRHLRENFLVPKIADLPPTDLSLVDGSQECIYLCGNSLGLQPKNARKYLEEELDNWAKIAIHGHTVGSRPWAWAENNLEDFMANIVGAKSGEVALMNGLTVNLHLLMLSFYKPTPTRYKILLESKAFPSDHYAIESQIRLRGFPPESSMLMLSPRPGEDTLRTEDILDVIKKEGDVIAMVMLPGVQYYTGQLFDMHAITKAGHKKGCFVGFDCAHAVGNADLKLHEWDVDFACWCSYKYLNSGAGGLAGAFIHEKHKDTIKPALLGWWGHDLATRFRMTNVMELQPGVSGFRLSNQPILLVCPLQASLELFNMASMQSLRRKSVLLTGYLEYLMKEFYTEDPAQPHKAYVRILTPSDPQQRGCQLSLSFSIPIRRVFQELEKRGVACDMREPSVLRVAPVPLYNSFRDVHRFIETLGKALAASSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSX5</accession>
    <name>A0A3B5MSX5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">small monomeric GTPase</fullName>
            <ecNumber evidence="5">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasmic vesicle membrane</location>
            <topology evidence="13">Lipid-anchor</topology>
            <orientation evidence="13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Phagosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSX5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026873"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027202.1">
        <property type="protein sequence ID" value="ENSXCOP00000026873.1"/>
        <property type="gene ID" value="ENSXCOG00000020041.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155363"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01867">
        <property type="entry name" value="Rab8_Rab10_Rab13_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000202">
        <property type="entry name" value="ras-related protein Rab-8A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="201" mass="23039" checksum="D235226E71CF572D" modified="2018-12-05" version="1">MAKTYDYLFKLLLIGDSGVGKTCLLFRFSEDSFNTTFISTIGIDFKIRTIELDGKRVKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDISNEKSFDNIKNWIRNIEEHASSDVEKMILGNKCDMTDRRQVSKDRGEKLAIDYGVKFLETSAKSGLNVEEAFYTIARDILNNLCSKTVGSEDILKKKMLLFFLMTLSLLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MT09</accession>
    <name>A0A3B5MT09_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nuclear receptor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="22"/>
            <end position="97"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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            <dbReference type="ARBA" id="ARBA00022771"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
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    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026903.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="124" mass="14227" checksum="64B2165AEDB7E6F4" modified="2018-12-05" version="1">MYCTVLRLAQSKGCEKDTQIEIIPCKICGDKSSGIHYGVITCEGCKGFFRRSQQSNAAYSCPRQKNCLIDRTSRNRCQHCRLQKCLAVGMSRDGELFYGRGIDRWVQRAKIPRGEELTFRHLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MT88</accession>
    <name>A0A3B5MT88_9TELE</name>
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        <recommendedName>
            <fullName evidence="9">E3 ubiquitin/ISG15 ligase TRIM25-like</fullName>
        </recommendedName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
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            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <end position="49"/>
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    </evidence>
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        </source>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="27030" checksum="88B2EE6734AEBFBA" modified="2018-12-05" version="1">METLSCSICLDLLKSPATIPCGHSYCMNCIKTHWDKEDQKGIYSCPQCRETFIPRPVLKKNTVFAALVDQMKTGLQATAADHCYAGPEDVACDFCTGRKLKAIKSCLVCLASYCEKHLQPHYDSSAFKKHKLVEPSKNLQENICSRHNEVKKMFCRTDQKCICSLCLIEEHKGHDTVSVAAERTERQRELEEKQKKIQQVLQEKEKDMXXXXXXXXXTLRTWQQLCQSSELNYRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MTV8</accession>
    <name>A0A3B5MTV8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DNA-binding protein SATB</fullName>
        </recommendedName>
        <alternativeName>
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        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1 15 16">Nucleus</location>
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        <text evidence="2 17">Belongs to the CUT homeobox family.</text>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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    </dbReference>
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        <property type="entry name" value="DNA-binding protein SATB"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="CUT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51983">
        <property type="entry name" value="CUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0160">Chromosomal rearrangement</keyword>
    <keyword evidence="11 15 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12 15 16" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="14 15 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="13 17" id="KW-0804">Transcription</keyword>
    <keyword evidence="10 17" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="CMP" evidence="21">
        <location>
            <begin position="61"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="CUTL" evidence="22">
        <location>
            <begin position="166"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="CUT" evidence="20">
        <location>
            <begin position="315"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="CUT" evidence="20">
        <location>
            <begin position="440"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="19">
        <location>
            <begin position="573"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="15">
        <location>
            <begin position="575"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="402"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="545"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="632"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="406"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="711" mass="78937" checksum="D17CFA7F7AD2579C" modified="2018-12-05" version="1">MEQCGGAGGGPESPDLPDRASPEERGAKPESPACSGPPQCKMSRLEVNGSPANPQIQQNGGLMIPIYCVVEHADAAGNCDSRSDRHAEFVLVRKDVLFTQLVEMALVALGYSHNSAAQARGIIKVGRWKPMPIHYLTDAPEATVADMLLDVYHMVTLRILLHSFSRLEDLPSEQWTHATVRNALKELLRETNQSTVAKESPLSQSMISAIVNSSYYATVSTSKCHEFGRWYKRYKPLPIKSTLGDASTLLGHGGLLSSQLSPQLVRQQLAMAHLINQQLAVSRLLAHQHPQGVNQQFLNHPPISRTCKVSGVAADPGLSCSGAEVSSDIYQQVRNELKRASVSQAVFARVAFNRTQGLLSEILRKEEDPRSASQSLLVNLKAMQNFLNLPEAERDRIYQEERERSTSTNHATTHISSSSSCSSSTIRHTQTKCTISGPELQLKLDSLANITSGIYEEIQQEMKRAKVSQALFAKVAANKSQGWLCELLRWKESPSPENRTLWENLCTIRRFLALPQVDRDQVYEEESRQQHSDRLHTVLHIPDQQPFHRHPLPPLTTPSPVHEEPHPGLGGGAKKSRSRTKISLEALGILQSFIGDVGLYPDQEAIHTLSAQLDLPKHTIVKFFQNQRYNVKHHSQTREDDQESSSPSEGGACATESREEEALSPSEELSEDGRGSVEMFRTERDVNDAKVNPFYHLFCISNNSVSVTTHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVH6</accession>
    <name>A0A3B5MVH6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">small monomeric GTPase</fullName>
            <ecNumber evidence="5">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Phagosome membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVH6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027571"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027905.1">
        <property type="protein sequence ID" value="ENSXCOP00000027571.1"/>
        <property type="gene ID" value="ENSXCOG00000020575.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158619"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04116">
        <property type="entry name" value="Rab9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000360">
        <property type="entry name" value="RAB9B, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041824">
        <property type="entry name" value="Rab9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF9">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB-9A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="220" mass="24944" checksum="987B2BFF9C81A311" modified="2018-12-05" version="1">ITPVWPLGVQPLTSHITAAMMSKSILLKVILLGDGGVGKSSLMNRYVTNKFDSHLFHTIGVEFLNRELEVDGRRVTLQIWDTAGQERFRSLRTPFYRGSDCCLLTFSLDDGQSFQNLSNWKKEFAYYADVKDPDSFPFVVLGNKLDVPERQVSGEEARRWCRENGGHPYFETSAKDATNVTSAFEEAVRRILVSEDRDDHLIQTDTVNLQKTPHPEPSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MW08</accession>
    <name>A0A3B5MW08_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Selenoprotein S</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the selenoprotein S family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027870826.1">
        <property type="nucleotide sequence ID" value="XM_028015025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MW08"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023769"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024053.1">
        <property type="protein sequence ID" value="ENSXCOP00000023769.1"/>
        <property type="gene ID" value="ENSXCOG00000017751.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114143205"/>
    <dbReference type="KEGG" id="xco:114143205"/>
    <dbReference type="CTD" id="55829"/>
    <dbReference type="GeneTree" id="ENSGT00940000166109"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036513">
        <property type="term" value="C:Derlin-1 retrotranslocation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036502">
        <property type="term" value="C:Derlin-1-VIMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009703">
        <property type="entry name" value="Selenoprotein_S"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28621">
        <property type="entry name" value="SELENOPROTEIN S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28621:SF1">
        <property type="entry name" value="SELENOPROTEIN S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06936">
        <property type="entry name" value="Selenoprotein_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="93"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="93"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="190" mass="21435" checksum="A5BA7BDD128DF994" modified="2018-12-05" version="1">MDDDVEILDVDDDNSPRQVPLKNQDLSQLSFTVGEFLSLYGWYLLAVTVTVLLLIQHLRKRRTGQSRQDSVPHNLQDVSLVTKRQEAMEAARRRMQAELDAKAAAFKEKQREQEEEKRRQKIEEWESMKQGKSSKGHKLSENTEEASAAAAGVKPKTDKKPLRSNDYNPLMGQGGSCTWRPGRRGPSAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MW65</accession>
    <name>A0A3B5MW65_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Golgi reassembly stacking protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GORASP2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GORASP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MW65"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025612"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025921.1">
        <property type="protein sequence ID" value="ENSXCOP00000025612.1"/>
        <property type="gene ID" value="ENSXCOG00000019135.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008686"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000026">
        <property type="entry name" value="Golgi reassembly stacking protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000056">
        <property type="entry name" value="Golgi reassembly-stacking protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007583">
        <property type="entry name" value="GRASP55_65"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024958">
        <property type="entry name" value="GRASP_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893">
        <property type="entry name" value="GOLGI REASSEMBLY STACKING PROTEIN GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893:SF1">
        <property type="entry name" value="GOLGI REASSEMBLY-STACKING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04495">
        <property type="entry name" value="GRASP55_65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51865">
        <property type="entry name" value="PDZ_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ GRASP-type" evidence="10">
        <location>
            <begin position="16"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ GRASP-type" evidence="10">
        <location>
            <begin position="112"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR607583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="344" mass="38051" checksum="D559DD08FF646885" modified="2018-12-05" version="1">DMGGSQSVQIPGGGSEGYHVLRVQEHSPGYHARLEPFFDFIISVCDTRLNRDNDTLKELLRVNVERPVKMLLYNSRTQTVRETTITPSNTWGGQGLLGLSIRFCSFERANQNIWHVLEVEPNSPAGLGDLRPYVDYIIGADTAMRESEDLFSLVEAHEGKELKLYVYNTDADNCREVIITPNSDWGGDGSLGCKIGFGYLHRIPTLKEEKNSRSPSQNHKASHIPKDGYTEVRRHVRGNSRVGLFNKYSQNLKDAFSLLKDKINLLMQLLFLLLSAGLPPLNLPAAVSSVTPPDFIYPPVAANSHTAIYPSSTIHMNSSLTKAPNHSSVPNSGAINFTVTVDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MX95</accession>
    <name>A0A3B5MX95_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Serine/threonine-protein kinase receptor</fullName>
            <ecNumber evidence="30">2.7.11.30</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="33 34">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-seryl-[receptor-protein] + ATP = O-phospho-L-seryl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18673"/>
            <dbReference type="Rhea" id="RHEA-COMP:11022"/>
            <dbReference type="Rhea" id="RHEA-COMP:11023"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25 30">
            <text>L-threonyl-[receptor-protein] + ATP = O-phospho-L-threonyl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44880"/>
            <dbReference type="Rhea" id="RHEA-COMP:11024"/>
            <dbReference type="Rhea" id="RHEA-COMP:11025"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="30">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="30">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane raft</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="30">Membrane</location>
            <topology evidence="30">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 30">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="30"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MX95"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026227"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026545.1">
        <property type="protein sequence ID" value="ENSXCOP00000026227.1"/>
        <property type="gene ID" value="ENSXCOG00000019587.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157527"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005026">
        <property type="term" value="F:transforming growth factor beta receptor activity, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14055">
        <property type="entry name" value="STKc_TGFbR2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23538">
        <property type="entry name" value="TFP_LU_ECD_TGFR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000260">
        <property type="entry name" value="TGF-beta receptor type-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000213">
        <property type="entry name" value="TGF-beta receptor type-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017194">
        <property type="entry name" value="Transform_growth_fac-b_typ-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015013">
        <property type="entry name" value="Transforming_GF_b_rcpt_2_ecto"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF55">
        <property type="entry name" value="TGF-BETA RECEPTOR TYPE-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08917">
        <property type="entry name" value="ecTbetaR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037393">
        <property type="entry name" value="TGFRII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00653">
        <property type="entry name" value="ACTIVIN2R"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="16 27 29" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0221">Differentiation</keyword>
    <keyword evidence="20 28" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="22" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="14 30" id="KW-0418">Kinase</keyword>
    <keyword evidence="17 30" id="KW-0460">Magnesium</keyword>
    <keyword evidence="23 30" id="KW-0464">Manganese</keyword>
    <keyword evidence="19 30" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 30" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 27 29" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21 30" id="KW-0675">Receptor</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 30" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="8 30" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 30" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18 30" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="32">
        <location>
            <begin position="220"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="178"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="31">
        <location>
            <begin position="185"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="26">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <begin position="226"/>
            <end position="234"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27 29">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="28">
        <location>
            <begin position="33"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="28">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="28">
        <location>
            <begin position="43"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="28">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="28">
        <location>
            <begin position="103"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047681"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048773"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR037393-2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR037393-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="RuleBase" id="RU361271"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="547" mass="62281" checksum="6C9838C5DEC1F513" modified="2018-12-05" version="1">MISHSDLLCRCDCSLKFGPVLFYTSMIAIKQLCRFCDLQATTCLGKGICKVDCDITSICPDDNDVCVSIWRKKDNNITFETVCHNPANPLYGLYLDDYNNTKCIMKEKKAKDSKFFICSCSKEEEDGFIRSVNVFILAVFVTDSQVGPVILVSLLPLLVIAIFVITSFYCYRIYSQQGNPRKKGKPLDNTDKHPIIDNEGSDSSSTHANNLNHNTELLPIELDVLVGKGRFAEVYKAKLKQGSSVREEHGFETVAVKIFQFEEYASWKNEKDIFSDADLRHENVLHFLTAEERKGQRQYWLITAYHPLGNLQEYLTNHIISWHDLWLLGSSLARVVAHLHSDRTSCSRFKVPIAHRDIKSSNILVKKDMTCCLCDFGLALRLDNSLSVDELANSGQVGTARYMAPEVLESRINLENIESFKQADVYSMALVLWEIISRCSAIGEVKEYKPPFGNLREHPCVESMKDSVLRDRGRPEIPNSWTSHMVCGSIEECWDHDPEARLTAHCIAERFDDVEYLDKLSDCSDSEEKMLEEIIVLETPHYKVGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXC6</accession>
    <name>A0A3B5MXC6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
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        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXC6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026277"/>
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        <property type="protein sequence ID" value="ENSXCOP00000026277.1"/>
        <property type="gene ID" value="ENSXCOG00000019627.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:protein ubiquitination"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="B30.2/SPRY"/>
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        <property type="entry name" value="SPRY DOMAIN CONTAINING SOCS BOX PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="SPRY DOMAIN-CONTAINING SOCS BOX PROTEIN 2-LIKE ISOFORM X1"/>
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    </dbReference>
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        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="1">
        <location>
            <begin position="31"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="241" mass="25915" checksum="86605D4B2895F0C2" modified="2018-12-05" version="1">MGLILSAWLNGPAADGVPSSSSAFIPLAVPTSSRVALILNSSAVSPGDRRSHWSSQHHSPHFQLSQCKQEVTRAPAELSSDGVRAEVGVESGLHVWEVTWNPEHRGSHAVIGISRQDCPLQDSGYKVLVGGDSLSWGWELQTNQLWHRGETLACFPCERRGSLRLKSSDSLHSKETDAPLLIPERVQLVLDADAGTLGFAVHGSFLGVAFRDLPPGVELFPAVSSVRGGARIVLRYLNGTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MYQ5</accession>
    <name>A0A3B5MYQ5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thymosin beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MYQ5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025505"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025812.1">
        <property type="protein sequence ID" value="ENSXCOP00000025505.1"/>
        <property type="gene ID" value="ENSXCOG00000019056.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282933"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:actin monomer binding"/>
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        <property type="term" value="P:actin filament organization"/>
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        <property type="term" value="P:regulation of cell migration"/>
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        <property type="entry name" value="Thymosin beta"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Single helix bin"/>
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        <property type="entry name" value="Beta-thymosin"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <source>
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        <source>
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    </evidence>
    <sequence length="36" mass="4183" checksum="A3A847E5429C0D2D" modified="2018-12-05" version="1">MSDKPDITEVTTFDKTKLKKTETQEKNTLPTKEIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N053</accession>
    <name>A0A3B5N053_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Poeciliinae</taxon>
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    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="PROSITE" id="PS50211"/>
        </source>
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    </evidence>
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    </evidence>
    <sequence length="1014" mass="115630" checksum="2026243324F5A96B" modified="2018-12-05" version="1">ERKAGSEDVGVPKNIDLMALPQLCFPGGLLVTGEEKKEQFHFLVFTDVFGNKTHGVVMQYYRSILVLLTLFYDKIIHILIQSYCFTCVSHFFSLFASLLFQLSSCRLSEMEEKLNEFAIKLSLVPIPPPGQLHLMFTLRPLTIVLPSSEDKSLQAVDLDLHLPFLCFRPQQLLLVLSSLLQEQRVVLFSADLARLTLVAESLLIFLQPLTWQHPYVPVLARGMLDFLMAPTAFLMGCHLSHYAEVAAETDDLISVNIDDGTVSTSLSDAIDIPEIPLAAADYFIQRSRSLQIHFDLGQYHSASCMGINEQRAQRRAWQHKLNHEIQKISLELLVNIFRDVCSHLNYEHRVFHSEEFLKTREPDELLFYKVVLDTHIFHSFLKDRLNKKMDYFARMELSTRAEIMSVVDVQRRPTMQEILTRRKSSVVGNKLSKRLGMSLPNLGGYQVINSEKSPLLTRGVMSDSVLKTPPDPVKVFKLPEFPVFLSSHSIQAYYSELIQLLGNAIFSVQTDNSSLLARFYYLRGFVNTLCSRRLDALSDFQSLNKTDTAIFPTHLVTWLVDSLHQDELQQAQTRPELKSFLFKPAVRTDNRIKKFKLPQTPLDQDEFVRCIQECGVVKDVATISRLFDALTDEQHVKVDPKVFKMFYTFWKETEAEAQDVNLLSEVISRLDDSECVYKLSSRVKTSYGVGKIAMTQKRLFLLTEGQPGFLEIAKFRDIQEVKMGSAPFLLVRILSLRIKTFSRPELFEANLKMETKLWNLIIKEMWAGRKMSDQDKDPQCMTQALTNVLLMDAVVGCLNPHRSISAASKLVYFDRILHEVHTIVPNTTSETLKHKINPSLDLAEPQTVCVLLYTPGQLWDKKSPIVMNPKLWVALSGGRVVVFDAASWSMLQDCIHTGELQVTCMMGLVPDQVWMGSQDSAIYIIDIHSMSCNKQLTEHRHEVTGLSVDTTDHSNGQLAYSCSCDGTILQWDSATLKVKRQFYLSCDRLCSIRFYDGVIWCCEYTLFIQSVISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KPD3</accession>
    <name>A0A3B5KPD3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027895449.1">
        <property type="nucleotide sequence ID" value="XM_028039648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KPD3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000327"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000334.1">
        <property type="protein sequence ID" value="ENSXCOP00000000327.1"/>
        <property type="gene ID" value="ENSXCOG00000000303.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114158286"/>
    <dbReference type="KEGG" id="xco:114158286"/>
    <dbReference type="CTD" id="566903"/>
    <dbReference type="GeneTree" id="ENSGT00950000183136"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010972">
        <property type="term" value="P:negative regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000065">
        <property type="entry name" value="G-protein coupled receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24234:SF7">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 132-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24234">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID RECEPTOR 5/SPHINGOSYLPHOSPHORYLCHOLINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="231"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="42"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="338"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="353" mass="39646" checksum="65A9B34770B8DD0D" modified="2018-12-05" version="1">MLNVTGGFSVNQSSVSCERPYGDDRLPLLVLYIVVLVVGLPANVLTLFLTWQQVRRKNVLGVYLWSLSLCDLTYLFTLPLWADYVNNGHEWRWSSTVCKLTGYIFFTNMYISIFLLCCISCDRYWAVAYSLESRGQRRLCHAVVITVVIVLVVALGHTAVFTMREGNVKSSKRCFEPTQSSVKVMGFNYARFVIGFLLPLLLLAVTNRRVLTNVQRSTGLHGDQKRRVRRLAVAVVLLFLVCFAPYHLILLARAVITQFPQLVGTPCLFEETMYSSYTISLGLSTINSAVNPILYVLSSNNIRKECRRGLTQICVSACHGPSTNSSQIKSLELNAGTDTERHPVGPEETLETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQ87</accession>
    <name>A0A3B5KQ87_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQ87"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000025"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000025.1">
        <property type="protein sequence ID" value="ENSXCOP00000000025.1"/>
        <property type="gene ID" value="ENSXCOG00000000022.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030510">
        <property type="term" value="P:regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
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        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Prot_Inhib_GrowthFact_Antg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913">
        <property type="entry name" value="FOLLISTATIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913:SF81">
        <property type="entry name" value="KAZAL-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017183762" description="Kazal-like domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="3">
        <location>
            <begin position="15"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="76" mass="8370" checksum="77BD934EBDCAE043" modified="2018-12-05" version="1">LKLSIISLFFKFGVSVASAPVCGSDGITYSNECELNRSACEHKRKIDVAKHGSCEEGRKWTLKCCNYVNQSAIEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KR07</accession>
    <name>A0A3B5KR07_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Death associated protein 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027879998.1">
        <property type="nucleotide sequence ID" value="XM_028024197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KR07"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000315"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000322.1">
        <property type="protein sequence ID" value="ENSXCOP00000000315.1"/>
        <property type="gene ID" value="ENSXCOG00000000289.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114148740"/>
    <dbReference type="KEGG" id="xco:114148740"/>
    <dbReference type="CTD" id="58094"/>
    <dbReference type="GeneTree" id="ENSGT00940000154574"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070513">
        <property type="term" value="F:death domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097190">
        <property type="term" value="P:apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="DAP1/DAPL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13177">
        <property type="entry name" value="DEATH-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13177:SF2">
        <property type="entry name" value="DEATH-ASSOCIATED PROTEIN-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15228">
        <property type="entry name" value="DAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="111" mass="12164" checksum="61ECD3C758D446B7" modified="2018-12-05" version="1">MVQQLSKSGAKEARLLKAGHPPAVKAGGKRVAKKTLEDGSAHGTPEKETKSRSLATSNRMQQVGLLLTGTLDKLSHDFPETPVSVRHSKVRPAVERSHCPRNFCIQQPRKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KR71</accession>
    <name>A0A3B5KR71_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSXCOT00000002653.1">
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    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:actin filament binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052223">
        <property type="entry name" value="Actin_Cytoskeleton_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17271:SF9">
        <property type="entry name" value="MYOSIN PHOSPHATASE RHO-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17271">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="50" mass="5966" checksum="73AC792311609F34" modified="2018-12-05" version="1">IMSLKENVCRKFQANIFNKSKCQNCFKPRESHLLNDEDLNQVLFMSINLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KSA1</accession>
    <name>A0A3B5KSA1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Milk fat globule EGF and factor V/VIII domain containing b</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KSA1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000805"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000815.1">
        <property type="protein sequence ID" value="ENSXCOP00000000805.1"/>
        <property type="gene ID" value="ENSXCOG00000000681.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156049"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000002">
        <property type="entry name" value="Coagulation factor VIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000226">
        <property type="entry name" value="EGF-like repeat and discoidin I-like domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050633">
        <property type="entry name" value="Neuropilin_MCO_CoagFactor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806:SF11">
        <property type="entry name" value="MILK FAT GLOBULE EGF AND FACTOR V_VIII DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
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        <location>
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            <dbReference type="PROSITE" id="PS50026"/>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="445" mass="49836" checksum="90C010D5202F9078" modified="2018-12-05" version="1">VWLYAGFSLFLILFVCVFLGVALALLAGDLCEVNVCNHGGTCVTGTGTPFICICPDGFSGETCNETETGPCSPNPCQNDGICEATGQSRRGDVFTEYVCKCQPGYEGVHCQTNVNDCADQPCENGGTCRDLDGDFKCHCPSPYVGKHCQLRCISLLGMEGGGIAESQITASSIRYTMLGLQRWGTELARLHNKGLVNAWSAAPHDKNPWIQINMQRTMRFTGIVMQGASRIGTAEFIKAFKVASSLDGRTYTMYRTEGQRRDQIFAGNVDNDSTKTNLFDPPIIAQYIRIIPVVCRKACTLRVELVGCELNGCSEPMGMKSRLVLDRQITASSTFRTWGMDAFTWLPQYARLDKQGKTNAWIPATNRRSEWLQVGGDAPETLFKFFYLIFWFVKLSFFIFTGNSDNNVHKKNIFEPPFYSRYVRVLPWEWHERITLRMELLGCDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTM3</accession>
    <name>A0A3B5KTM3_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <begin position="1"/>
            <end position="24"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001852.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="115" mass="12817" checksum="9C391CADACFB0EB6" modified="2018-12-05" version="1">MAHVHQGGRGHKDHLEHPVPDQGDGECLVVADVTAARLLRVADEVRLFVVPHILSSHTQHQHPEDEQDSKPDLAYHGGVDMDLLQDPTKEVPVSHFFTLAGLELVEKHTFRIVNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KUZ9</accession>
    <name>A0A3B5KUZ9_9TELE</name>
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        <submittedName>
            <fullName evidence="4">Deleted in azoospermia-like</fullName>
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        <name type="scientific">Xiphophorus couchianus</name>
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        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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            <authorList>
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        <property type="project" value="Ensembl"/>
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        <location>
            <begin position="60"/>
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        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="230" mass="25547" checksum="9D7550FAF06800B5" modified="2018-12-05" version="1">MLAKNGGIFPLANVSLTKNEFNLYSRLDSPKKKSETITQTVPSLKLSNGYILPEGRLTPNALFVGGIDMKVDENEMRDFFTRYGNVKEVKIITYRGGVCKGYGFVYFNEDVNIQSIIEQQISFKGRKLKLGPAIMKQRSPRSIPSRLVGSTPWMSPSQYIYCACCSPMGGGVTQPSPVYSGGNPYNQPYSYSSFSGVMVPQMPMSYAQNPYPYQVNSFVDCGVQTMLTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KWM0</accession>
    <name>A0A3B5KWM0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
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    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
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            <taxon>Poeciliinae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="ENSXCOP00000002927.1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="Si:dkey-126g1.7"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
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        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
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        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="8">
        <location>
            <begin position="169"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="7">
        <location>
            <begin position="778"/>
            <end position="972"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="972" mass="110642" checksum="8BF836A72070FE98" modified="2018-12-05" version="1">MYVLVLGLDVLGNPFGVIRGLSEGVEAFFYEPFQVKHLAYCEIHMKPDQGGGSDGRGSVCLPGSRSGSRGVSRMFSVVSETQAAICQVKLKSSLAKRCQYLFEGNSWRGKLTPVNQIYTELNVTEQETGEVDNEHEVQQTEAASLKPDDPETTISCEDIFKPGRDEQIRTLVTKGAAGIGKTLLTQKYTLDWAEGKTNRNIQFIFPFTFRELNLLKSQSITLVGLIHQFFPEIRKAGICSFEEFQIVFIFDGLDELQPPLDFHHTPVLIDVTEPSSVHTILVNLIRGKLLPSAHLWITTRPAAVHQIPIQYVDSEKEIRGFTDQRKKEYFRKRFQDKEHTNKIISHIETSRSLHTMCHIPVFCWITATVLDQVLKENKKTEMPKTLTELYIHFLVVQVSQDNEKYHKKVLTTDAIWNPETEKIFLAIGKLAFGQLEKRNLLFYEEDLKEYGIDINTASAYPGVFTETFKEESVLNRKRLFCFVHRSIQEFLAALYVFLVFMKTGDNLMRRSRSALQPESKFKQFYQSAVDKALESPDGYLDMFTRFLLGLSLKTNQDLLQGLLQKTKNAPQVQKTLVQHIKKNIRVCPSVEKSIRMFHWLNELNDHSLEEEIRLYLSGKVLEKELTSSQWSALIFVLLSSQKVLDVFDFKKFSKSKEALVWLAPVVKASKKSLYVSHKVQENRSSIHVVPVLTAENSCLRELDLSDNDLHDEGVDLLSFGLRSPKCRLKVLSLSGCLVTEKGCDYLASALTSNPSYLRELDLSFNHPGESGLDAFSAGADHCGKHRLNPSPQRFFLEQLLDPNTANRNLVLLPDNKHVKAVKEEQPYPDHPERFDTWKQVLCTDGLTGRCYWEVQWIGTVRIGLSYKGIRRKGEDDECCIGGNDQSWALSCSPQSFTAWHNNTPTEIKPLEKTNSNRLAVYLNWSAGTVSFYSLPGIVSSIKKIHLHTFETTFTEPLYAKHHHQPLLCCNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXF4</accession>
    <name>A0A3B5KXF4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTH2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="RefSeq" id="XP_027857560.1">
        <property type="nucleotide sequence ID" value="XM_028001759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXF4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003232"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003269.1">
        <property type="protein sequence ID" value="ENSXCOP00000003232.1"/>
        <property type="gene ID" value="ENSXCOG00000002547.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114134895"/>
    <dbReference type="GeneTree" id="ENSGT00940000165995"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1490.10:FF:000002">
        <property type="entry name" value="Peptidyl-tRNA hydrolase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="entry name" value="Bit1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649:SF29">
        <property type="entry name" value="AMINOACYL-TRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22562">
        <property type="entry name" value="UBA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="Peptidyl-tRNA hydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBA" evidence="5">
        <location>
            <begin position="17"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="184" mass="20206" checksum="9B96E8A50A29AE69" modified="2018-12-05" version="1">MEPSEDTGSNLASQEVNPVFLQQLRELDIPEEAAKQALLHTRNVSAEEAAMYYFNKLENEEEGDEDLLFKMVFVVNMDLGMGVGKIAAQVGHAAVGLYQALQEKNSWREMAWKWDQSGAKKVVLQGTNVAHLLELQALALSLNLPTYLVQDAGLTQVESGSRTVLAIMGAEEMVNNVTGSLKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYC6</accession>
    <name>A0A3B5KYC6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Microfibril associated protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-mRNA splicing as a component of the spliceosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the spliceosome B complex. Interacts with PRPF38A (via N-terminal interaction domain).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MFAP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KYC6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003562"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003601.1">
        <property type="protein sequence ID" value="ENSXCOP00000003562.1"/>
        <property type="gene ID" value="ENSXCOG00000002808.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00690000102225"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033194">
        <property type="entry name" value="MFAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009730">
        <property type="entry name" value="MFAP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15327">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06991">
        <property type="entry name" value="MFAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Micro-fibrillar-associated protein 1 C-terminal" evidence="6">
        <location>
            <begin position="190"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="43"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="202"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="131"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="437" mass="51788" checksum="F7CBBC3E708B1B1C" modified="2018-12-05" version="1">MSSHEAANMKLPPIQSTAGAVPIRNEKGELTMEKVKVKRYVSGKRPDYAPMESSDEEDEEFKFVKKGVEAEPEVEPQEEDVSDPRLRRLLNRTSEDVEERLARHRQIVEPEVVAESSEDSDEGTWHPEREDSSEEEEEEEEEEIERRRAMMRQRAVERKNEEMEVMEVEEEVKSGEESESESEYEEYTDSEDEAEPRLKPVFIRKKDRITVAEREAEELKQRELEAEAKRQTEERRRYTLKIVEEEAKKEFEENRRTLAALEALDTDGENEEDEYEAWKVRELKRIKRDRETRETMEKEKSEIERFHNLTEEERRAELRNSGKVVTNKAQKGKYKFLQKYYHRGAFFMNEEEDVYKRDFSAPTLEDHFNKTILPKVMQVKNFGRSGRTKYTHLVDQDTTSFDSAWAQESAQNSKFFKQKAAGVRDVFDRPTVKKRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZL1</accession>
    <name>A0A3B5KZL1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein phosphatase CPPED1</fullName>
            <ecNumber evidence="4">3.1.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Calcineurin-like phosphoesterase domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallophosphoesterase superfamily. CPPED1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZL1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003992"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004036.1">
        <property type="protein sequence ID" value="ENSXCOP00000003992.1"/>
        <property type="gene ID" value="ENSXCOG00000003135.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165873"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07395">
        <property type="entry name" value="MPP_CSTP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041867">
        <property type="entry name" value="MPP_CSTP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051918">
        <property type="entry name" value="STPP_CPPED1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43143">
        <property type="entry name" value="METALLOPHOSPHOESTERASE, CALCINEURIN SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43143:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE CPPED1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="12">
        <location>
            <begin position="76"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="314" mass="34977" checksum="9703D15316B1BA6F" modified="2018-12-05" version="1">LLGSDPVFWVGTALTRCVGASPEAERQWTGPFCFVQAADPQLGLMKAWRIGDAVGGGDEWEEEVALTKQAVAGVNQLRPRPRFMVLCGDLVHAMPGTEFREAQERDLKEALRETDPSIPLVFVSGNHDLDQVPTPSTVEQYCRAWGDDYFSFWVGGVLCLVLNSQLYFNASACPQQKEAQETWLEAQLSRASSAEPKPKHILVFQHIPLYVKSHDEQDEYFNLPQETRQNLMERFKRAGVTAVFSGHYHRNAGGCHDGLDMVVTSAIGCQLGDDTHGVRLVAVTANAVVHRYYSLEQLGQRGVDEELRKMLQAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L129</accession>
    <name>A0A3B5L129_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L129"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004502"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004558.1">
        <property type="protein sequence ID" value="ENSXCOP00000004502.1"/>
        <property type="gene ID" value="ENSXCOG00000003560.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175860"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029073">
        <property type="entry name" value="DUF4661"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14307">
        <property type="entry name" value="C6ORF47 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14307:SF0">
        <property type="entry name" value="SI:CH73-25F10.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15576">
        <property type="entry name" value="DUF4661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="256" mass="28932" checksum="0E4F36A75D1A3504" modified="2018-12-05" version="1">MESFVTSHNASDVSSYAGHTFGMLDVGTSTRAVAARLNIHYRTIGRLTKGSQLKGTELQVSSWWSKYLPTSYLWSRKPPQSSLRQRKGDVDGDFSDYGTPPPSPTPSQLTSPFRFFTDSWNLEIIPEHYEICFNFLRHLFDLFVVAFLWTVSPPAKLILEVFGVQGALRLWFHGMAMFFVSTAGMAGLLWLIQEYLPQFALIYGIIQALVISVSVRQSVILGVEEDKENSQEVKETGEKQDTQMTWHPKPSLTVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1P8</accession>
    <name>A0A3B5L1P8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Hormone-sensitive lipase</fullName>
            <ecNumber evidence="11">3.1.1.23</ecNumber>
            <ecNumber evidence="10">3.1.1.79</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Monoacylglycerol lipase LIPE</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Retinyl ester hydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="45 46">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="45" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="45" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = di-(9Z)-octadecenoylglycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38575"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:75945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:38576"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>1,2-di-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+) = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O</text>
            <dbReference type="Rhea" id="RHEA:38379"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:52323"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="34">
            <dbReference type="Rhea" id="RHEA:38381"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>1,2-di-(9Z-octadecenoyl)-glycerol + H2O = (9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38455"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:52323"/>
            <dbReference type="ChEBI" id="CHEBI:75937"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:38456"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>1,2-di-(9Z-octadecenoyl)-glycerol + H2O = 2-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38659"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:52323"/>
            <dbReference type="ChEBI" id="CHEBI:73990"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:38660"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + H2O = (9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39935"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:75937"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="41">
            <dbReference type="Rhea" id="RHEA:39936"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>1,3-di-(9Z-octadecenoyl)-glycerol + H2O = 1-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39939"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75342"/>
            <dbReference type="ChEBI" id="CHEBI:75735"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:39940"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>1-(9Z-octadecenoyl)-glycerol + H2O = glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38487"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75342"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:38488"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1-O-hexadecyl-2-acetyl-sn-glycerol + H2O = 1-O-hexadecyl-sn-glycerol + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38563"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:34115"/>
            <dbReference type="ChEBI" id="CHEBI:75936"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:38564"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>2,3-di-(9Z)-octadecenoyl-sn-glycerol + H2O = 2-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38383"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:73990"/>
            <dbReference type="ChEBI" id="CHEBI:75824"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:38384"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = glycerol + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:52392"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:26133"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>2-(9Z-octadecenoyl)-glycerol + H2O = glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38491"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:73990"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="40">
            <dbReference type="Rhea" id="RHEA:38492"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Hydrolyzes glycerol monoesters of long-chain fatty acids.</text>
            <dbReference type="EC" id="3.1.1.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>a diacylglycerol + H2O = a monoacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17408"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.79"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>a monoacylglycerol + H2O = glycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15245"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17408"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.79"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.79"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>all-trans-retinyl hexadecanoate + H2O = all-trans-retinol + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13933"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:17616"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="36">
            <dbReference type="Rhea" id="RHEA:13934"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>cholesteryl (9Z-octadecenoate) + H2O = cholesterol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33875"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:46898"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:33876"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Glycerolipid metabolism; triacylglycerol degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Lipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="27">Monomer and homodimer. Interacts with CAVIN1 in the adipocyte cytoplasm. Interacts with PLIN5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <location evidence="4">Caveola</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.23" evidence="11"/>
    <dbReference type="EC" id="3.1.1.79" evidence="10"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001729"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001755.1">
        <property type="protein sequence ID" value="ENSXCOP00000001729.1"/>
        <property type="gene ID" value="ENSXCOG00000001396.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111056"/>
    <dbReference type="UniPathway" id="UPA00256"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004771">
        <property type="term" value="F:sterol ester esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010468">
        <property type="entry name" value="HSL_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025:SF2">
        <property type="entry name" value="HORMONE-SENSITIVE LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23025">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06350">
        <property type="entry name" value="HSL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="19" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="17" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="21" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="46" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="23" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="22" id="KW-1207">Sterol metabolism</keyword>
    <feature type="domain" description="Hormone-sensitive lipase N-terminal" evidence="43">
        <location>
            <begin position="7"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="44">
        <location>
            <begin position="350"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="44">
        <location>
            <begin position="606"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="active site" evidence="42">
        <location>
            <position position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00013088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00013254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00015845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00030031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00031112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00046695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00047438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00047458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00047476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00047653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00047674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00048386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00048657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00048674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00049053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00049143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00049208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00049372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="ARBA" id="ARBA00049461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="ARBA" id="ARBA00049519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="43">
        <source>
            <dbReference type="Pfam" id="PF06350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="44">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="45">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="46">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="703" mass="76770" checksum="D431C2D9051FD6E2" modified="2018-12-05" version="1">MDHSAVFTALDKVCEENICVLSGPSESPYGTVTKRLVACMRKIRQHGRALEPVVASFTAVYHHYDFDAQTPANGYRTLVKVLQSCLVHIIHKGRYIASNCRGAFFRAEHNAAEMEAYCSALCQLRALLHLAQRLIHDNAHGHLYSPQDADLSDRFVQEYSSMHKACFYGRCLGFQFSPALRPFLQTIVISMVSYGETYGRQQSGIGTAALSLLTSGKYVIDPELRGAEFERITQNLDLHFWKSFWNVTESDILSGFTRIASNPVQVNLTLTVPPESLRLPLASDPTLSTSVSPPVAHWGPGPVHMRLISYDLRDGQDSEELLGFSPGDVAPIASPRLPWLQKKPPSPWLLIHFHGGGFVAQTSRSHENYLRNWSKELSVPVLSVDYSLSPEAPFPRALEECFYAYCWALKHCHLLGSTAERVCLAGDSAGGNLCITVSMKALTAGIRVPDGIMAAYPATLLTTDASPSRLLTLIDPLLPLGVLSRCINAYAGSDFQTIQPSERRGSLSALGRDTALLLSDLTQGASNWIQSLLDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAGAAAAGAANILHYPDGFEPLRSECHAYVLPTSYPVIRNPFVSPLLAPTGLLKGLPPVHLVASALDALLDDSVMFAKKLRAMGQAVTLTVVDDLPHGFLSLAQICKETQFACDICVSRIRMVFQPDSAGASPQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L221</accession>
    <name>A0A3B5L221_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L221"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006426.1">
        <property type="protein sequence ID" value="ENSXCOP00000006348.1"/>
        <property type="gene ID" value="ENSXCOG00000004908.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="63" mass="7020" checksum="F59B7217BF52DAC9" modified="2018-12-05" version="1">MPLAQIAEPWPTVELVQLDTEVRKCEINGLLCTCVASFSPNRVVPFKERELISFSLKSGLSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2T1</accession>
    <name>A0A3B5L2T1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RWD domain containing 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2T1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004670"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004727.1">
        <property type="protein sequence ID" value="ENSXCOP00000004670.1"/>
        <property type="gene ID" value="ENSXCOG00000003685.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015545"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd23817">
        <property type="entry name" value="RWD-RWDD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RWD_dom"/>
    </dbReference>
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        <property type="entry name" value="RWDD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21275">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21275:SF1">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="2">
        <location>
            <begin position="9"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="182" mass="20852" checksum="71015C2E229E6CD3" modified="2018-12-05" version="1">MTANEDQEMELEALRSIYEGDECFKEISPVSFQFRVGDLEDTKAFILDISWPDTYPETAPQISLDAFFNNRISAETKQLILSKLEPQVEASLGSAMMYTLFEWAKENQEELMENHRPVVTALTSNTEVTTPSAAKKREKKEQLTKAQKRRMINRTDNKGELPRGWNWVDVIKLSKTGGKDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L3H2</accession>
    <name>A0A3B5L3H2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cortactin-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Regulates the dendritic spine distribution of CTTN/cortactin in hippocampal neurons, and thus controls dendritic spinogenesis and dendritic spine maintenance. Associates with the striatin-interacting phosphatase and kinase (STRIPAK) core complex to regulate dendritic spine distribution of the STRIPAK complex in hippocampal neurons.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with CTTN/cortactin SH3 domain. Interacts with STRN, STRN4/zinedin and MOB4/phocein; this interactions mediate the association with the STRIPAK core complex and may regulate dendritic spine distribution of the STRIPAK complex in hippocampal neurons. Activation of glutamate receptors weakens the interaction with STRN and STRN4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendritic spine</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000005357"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005418.1">
        <property type="protein sequence ID" value="ENSXCOP00000005357.1"/>
        <property type="gene ID" value="ENSXCOG00000004026.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158293"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050807">
        <property type="term" value="P:regulation of synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019131">
        <property type="entry name" value="Cortactin-binding_p2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057568">
        <property type="entry name" value="CortBP2_NAV1-like_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050889">
        <property type="entry name" value="Dendritic_Spine_Reg/Scaffold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166:SF27">
        <property type="entry name" value="CORTACTIN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24166">
        <property type="entry name" value="ROLLING PEBBLES, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25408">
        <property type="entry name" value="AAA_lid_NAV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09727">
        <property type="entry name" value="CortBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 14" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Cortactin-binding protein-2 N-terminal" evidence="16">
        <location>
            <begin position="12"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="593"/>
            <end position="625"/>
        </location>
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    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="626"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="659"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="692"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="domain" description="CortBP2/NAV1-like AAA+ ATPase lid" evidence="17">
        <location>
            <begin position="1064"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="144"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="197"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="254"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="294"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="376"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1140"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1207"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="519"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1144"/>
            <end position="1156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF09727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF25408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1423" mass="152764" checksum="369B1784100B3D77" modified="2018-12-05" version="1">LCNNSNIGFFLKEVFLQDRYGHYTLTDPFLALQRDFDCRVPGGDKERRPLGSSPISILEAVMAHCRRMQERMSAQLAAAESRQKRLEMEKLQLQSLEQEHRKLTAQLKDEREKNKHIVMMLVRECKQLATRVVEESQRFDELQARLDEESRTSGRLQEELGGERQRSHQMEAKMEKQLSEFDTECEQLRARLGRVEAESQGLRQQVEQLRTERSDEKDDAAAASPAAATSTPPKPKAMISVSIATESISCQAASCQTDVPPTEAEGPKKTPLSIPAKPAPANYVGLSLPKTTGRGIVHSSSGGLAQTENGSEGQTTHGLPTGVSPRVQAARYKFQEQDQNGTASQSPPARDLSPTNRDNFAAKQQARHTVTQVLSRFTSPPAGGAGPLRPALPHSASEGGPFPGRLSHPIGLKSPTVTRIDRGNPPPIPPKKPGLSQTPSPPHPSIKVVGESSRSPGAGLKPATPQLPPKPALDLVPALTASQVGACPRSPGPGRGPQRQAATACAESLQDISPATTVSSPSSIHPPSTSISAPSSYSSRASAGSPLATASGWCPSIVSSLSSGGPAALDSRPLLLLQAASQGNVTLLSMLYNQTSSLFAAAQNGQTECVKLLLSAGLPADILDENGFTPLHLAAAHGHASCVEVLLASRAAMDPVAAQGQTPLFLACEAGNLDCAQALLTAGANRSLPTTDGCTTLHAAVRSGHVDALRLLLHHGDPPDTLDGSALSAALLNQANSDGWTPAHMAAALGLKECLEVLCSHIEQDIERRDKCNRTIHDVATDDCKDLLENLGEYFFSYLCFNRCSAVEIGPMCPMGAVEDEEEAKVLSCSQTALGRLTVDRGTCWAQLSAALSSMFISHLQILCGEDAQRSLLGLTPASISSILIGTLSLGSNVICFLFDVQVEQYGNLFFHGLEGSCQEYIANLVAHCIKVRPSGCFLVPAAGSGFGEVCGYSDQCVVVLLEGVEKASSLTGLLGDLCLSLDNRGSASPLILSTGTTNTRLSLHHVRENSFLIGTLSKPRLQGSELRLQQHFRWVMLRWDQEPLHGLLGRHLRRKVLHKVGQRSADGLMERSVLWVSQVWQQLNACLSRLGTHEALLGPQLFLSCPVVPSNAQPIVRWLARLWNAVVVPRVEGAVISRVTTKRSTSSPSQRLSPSNCGLSTGQQAVVKAALSILVNKAVMPGCPLPRHEMDRYLLEFQGGSSPLPALSSCREGAGGGRRGRDGSKLRRSNTSPRKKGSPSVTWSSGGSFREGQYRKQHLGPTLTSSSTGLPVFFDDETDLIRELQTMCSSRSEPDISQSAVCKFNKQYVCSNCHHGLTDNQTSLAPPCGSTAICSNEMHRLAETTNQSQEEGLNAINQLCVNAPALLMAVILRCQGKRQFHLCREASESCIICTTLCWAVTLNLFKINVCGCDLIKCKVQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L438</accession>
    <name>A0A3B5L438_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CDK5 regulatory subunit associated protein 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDK5RAP3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L438"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004606"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004662.1">
        <property type="protein sequence ID" value="ENSXCOP00000004606.1"/>
        <property type="gene ID" value="ENSXCOG00000003638.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000713"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008491">
        <property type="entry name" value="CDK5RAP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14894">
        <property type="entry name" value="CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14894:SF0">
        <property type="entry name" value="CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05600">
        <property type="entry name" value="CDK5RAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="496" mass="56729" checksum="3CF2CE82D2F310C0" modified="2018-12-05" version="1">MFRLFLFYSSFHQNIQNLPIDIQTSKLLDWLVDRRHCNIKWQSTVKTVREKINTAIQDMPENEEIKQLLSGSYIHYFHCLRIVEILKGTEASSKNIFGRYSSQRMKDWQEIVSLYEADNVYLAEVASLLFRNVSYEGPALRKQIAKAKQLQQELSRREVECQSSAAELRERYYAACKQYGITGENVQRELQALVKDLPAVLEEVGKDAAKLEEQIQLYAAFTNFVCDWSKPVLPMLTFSQKAGNVTFYEWRTGKVPSLVERPAVEEAPPDAATEDTIDWGDFGNKPSDDLGVTAGITVEEGIDWGISLEPTSEVRQTNISPEGVARGDDALSILENSQSRSQFIDELMELEAFLTQRVTEMGEEGDVVAMSQFQLAPSIIQSQTREHIQELLSQVQDLLGRLTSLRMQHLFMIQASPRYVERVSEMLRQKLKQADILVLKAVTMVERREEALQEQSRLEPCVDLLAGRTRELQKLIEADISKRYHNRPVNLMGVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4H1</accession>
    <name>A0A3B5L4H1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAUS augmin-like complex, subunit 7</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000002659"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002694.1">
        <property type="protein sequence ID" value="ENSXCOP00000002659.1"/>
        <property type="gene ID" value="ENSXCOG00000002121.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003937"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070652">
        <property type="term" value="C:HAUS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031023">
        <property type="term" value="P:microtubule organizing center organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029711">
        <property type="entry name" value="Haus7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14352">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14352:SF2">
        <property type="entry name" value="HAUS AUGMIN-LIKE COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="299" mass="33088" checksum="F9BB9554F138E096" modified="2025-10-08" version="2">MXXXLTAIQGENGGGFDRAAALSCPLVQDLHLQEEESMLELLCRPSALRTDILTWICRRYTHLTPHRVIGKLIAEMAAMGQELMLCKADDLDLIKGKASPQRQLGLLEQLLSLVAGSDGRRTDVEALMCEVFAAENLPHLRQMLQPALDPWPAHIEYVCCAALSTIRADQTGCLVPSQSINATAVQTFTSACCCRSQCDFLNGAEENPAAVSPSSLRLAAGDLQQQMMTFSHLYETDLRVYCSREPPSFSAETDLFQRVQQQLLACNTVLNSCVTALNWGSTMIDLLFRALYHVIYQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5L1</accession>
    <name>A0A3B5L5L1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ess-2 splicing factor homolog</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESS2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5L1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005690"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005756.1">
        <property type="protein sequence ID" value="ENSXCOP00000005690.1"/>
        <property type="gene ID" value="ENSXCOG00000004427.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009387"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019148">
        <property type="entry name" value="Nuclear_protein_DGCR14_ESS-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12940">
        <property type="entry name" value="ES-2 PROTEIN - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12940:SF0">
        <property type="entry name" value="SPLICING FACTOR ESS-2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09751">
        <property type="entry name" value="Es2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="91"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="411"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="91"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="452"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="465"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="474" mass="52743" checksum="4558580CE118F786" modified="2018-12-05" version="1">MEGSASVRRALSGTLVPSAVTTVALCQPEDKEEGKLKRKVLDEEDYIQSLEKIIQRDFFPDITKLQAQREYLEAEESGDLEKMREISIRYGSSLNKSTPQTSAPYLTPASFETPVGRSGSPSSTYCSKEDIRRKDDDKEEKELPSLDRFLAKNTSEDNASFEQIMDLAKDKEKLKHAWLYEAEAEFKQRHEENLALPSMEKAALECVKAGLETWEYKAKNALMYYPEGVKDDDAIFKKPREVVHRNTRFVGDPFSKALNKSQIQQAAALNSQFKQGKVGPDGKELIPHESSTVNGYGFERTPSPAPGVADSPLMTWGEIESTPFRLDGSDTPYVERNHGPSFKIPEPGRRERLGIKMANEAAAKNRAKKQEALRKVTENLASLTPKGLSPALTPALQRLVNRTSSKYTDKALRASYTPSPSHRVIPCKSPFAGPATPSGTPTPTKAKTPSSQDLTSLTDNLLQLPKRRKASDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5L8</accession>
    <name>A0A3B5L5L8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sterile alpha motif domain containing 11</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5L8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006107"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006177.1">
        <property type="protein sequence ID" value="ENSXCOP00000006107.1"/>
        <property type="gene ID" value="ENSXCOG00000004726.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000235135"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10417">
        <property type="entry name" value="GLUCOCORTICOID MODULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10417:SF15">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN-CONTAINING 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="50"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="83" mass="9276" checksum="B40706012DE6BC5E" modified="2018-12-05" version="1">MSKGILQVHPPICDCPGCRISSPVNRGRLAEKRTIPLPPNRVPKKELASIFSSDDSEGSERASGDHAHIKQEEDLHYSIMRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5Q1</accession>
    <name>A0A3B5L5Q1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Component of a complex that binds and activates STK11/LKB1. In the complex, required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mo25 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5Q1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005730"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005796.1">
        <property type="protein sequence ID" value="ENSXCOP00000005730.1"/>
        <property type="gene ID" value="ENSXCOG00000004464.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004360"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000025">
        <property type="entry name" value="Calcium-binding protein 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013878">
        <property type="entry name" value="Mo25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182:SF11">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10182">
        <property type="entry name" value="CALCIUM-BINDING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08569">
        <property type="entry name" value="Mo25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="338" mass="39480" checksum="18155FA96772F159" modified="2018-12-05" version="1">MPFPFGKSHKSPADIVKNLKDSMTVLEKHDISDKKAAEEVSKSLVAMKEILYGTNEKEPQTEAVAQLAQELYNSGLLSTLIADLQLIDFEGKKDVAQIFNNILRRQIGTRTPTVEYLCTQQNILFMLLKGYESPDIALNCGIMLRECIRHEPLTKITLWSEQFYDFFKYVEMSTFDIASDAFATFKDLLTRHKLLSAEFLEQHYDKFFIEYEKLLHSENYVTKRQSLKLLGELLLDRHNFTIMTKYISKPENLKLMMNLLRDKSRNIQFEAFHVFKVFVANPNKTQPILDILLKNQAKLIEFLSKFQNDRTEDEQFNDEKTYLIKQIRDLKRPAPQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L637</accession>
    <name>A0A3B5L637_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the synuclein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L637"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003234"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003271.1">
        <property type="protein sequence ID" value="ENSXCOP00000003234.1"/>
        <property type="gene ID" value="ENSXCOG00000002549.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183175"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903136">
        <property type="term" value="F:cuprous ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.700">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001058">
        <property type="entry name" value="Synuclein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13820:SF5">
        <property type="entry name" value="ALPHA-SYNUCLEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13820">
        <property type="entry name" value="SYNUCLEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01387">
        <property type="entry name" value="Synuclein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01211">
        <property type="entry name" value="SYNUCLEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118375">
        <property type="entry name" value="Synuclein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="118" mass="11814" checksum="07E5C0E243EF5506" modified="2018-12-05" version="1">MDALMKGFSKAKDGVVAAAEKTKQGVTGAAEMTKDGVMYVGTKTKDGVSTVAEKTVSGVSHVGGAVVTGVTAVAQKTVEGAGNIAAATGLVKKDPAKQTDKGSPTEEQTVDTVMLCPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6U3</accession>
    <name>A0A3B5L6U3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP synthase subunit b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9 10">Subunit b, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the eukaryotic ATPase B chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6U3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006160"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006231.1">
        <property type="protein sequence ID" value="ENSXCOP00000006160.1"/>
        <property type="gene ID" value="ENSXCOG00000004758.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001958"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2210:FF:000003">
        <property type="entry name" value="ATP synthase subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008688">
        <property type="entry name" value="ATP_synth_Bsub_B/MI25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013837">
        <property type="entry name" value="ATP_synth_F0_suB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12733:SF3">
        <property type="entry name" value="ATP SYNTHASE F(0) COMPLEX SUBUNIT B1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12733">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE B CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05405">
        <property type="entry name" value="Mt_ATP-synt_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161060">
        <property type="entry name" value="ATP synthase B chain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0138">CF(0)</keyword>
    <keyword evidence="4 10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="241" mass="27102" checksum="407EEC45D23197BB" modified="2018-12-05" version="1">LNHRFPLFASGSLKSSGPIGAGLVQASRSLQTSSQSLAPVPPLPEYGGKVRHGLIPEEFFQLLYPKTGVTGPYMLGTGLLVYLLSKEIYIINHETIAAITIGTIIVYGVKKFGPSVAAFADKLNEDKMAKAQEAKDVAMSSLTQAIENEKKEQWRVEGRAMLFDAKRNNVAMLLETNYRERLHMVTNEVKRRLDYQIALQHLNRRMQQEHMINWVEKNVIGSITPQQRVIHKCFSDHLSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7S0</accession>
    <name>A0A3B5L7S0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Protein FRG1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Binds to mRNA in a sequence-independent manner. May play a role in regulation of pre-mRNA splicing or in the assembly of rRNA into ribosomal subunits. May be involved in mRNA transport. May be involved in epigenetic regulation of muscle differentiation through regulation of activity of the histone-lysine N-methyltransferase KMT5B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
            <location evidence="1">Z line</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Cajal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FRG1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L7S0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006872"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006960.1">
        <property type="protein sequence ID" value="ENSXCOP00000006872.1"/>
        <property type="gene ID" value="ENSXCOG00000005312.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004552"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055120">
        <property type="term" value="C:striated muscle dense body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23338">
        <property type="entry name" value="beta-trefoil_FSCN_FRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000031">
        <property type="entry name" value="FRG1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008999">
        <property type="entry name" value="Actin-crosslinking"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010414">
        <property type="entry name" value="FRG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12928">
        <property type="entry name" value="FRG1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12928:SF0">
        <property type="entry name" value="FSHD REGION GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06229">
        <property type="entry name" value="FRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50405">
        <property type="entry name" value="Actin-crosslinking proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0517">Myogenesis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00059473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="252" mass="28052" checksum="AD53484936464665" modified="2018-12-05" version="1">MSEYSHVKSTKLVLKGQNKGFIKGHFSWPHTVPWFLCSPGGWWCVSSFGDVAGTVAIEMQNNSYIHALDTGLFTLGAPHGDDEGPDPPEQLTAIKLSDTRIAFKSGYGKYLGINSDGLVMGRSDAIGSREQWEPVFQDGKMALLAANGCFISYSDDGDIVANSKTAGDAEMLKIRTSTEREVKHKDELADEDRGNVKSCEVNYVKKFQSFQDRRLRVNEDDASTLKKARVDGKFHEALLDRRSKMKADRYCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L871</accession>
    <name>A0A3B5L871_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GRB2-associated binding protein 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the GAB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L871"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008498"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008604.1">
        <property type="protein sequence ID" value="ENSXCOP00000008498.1"/>
        <property type="gene ID" value="ENSXCOG00000006512.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157792"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005068">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000166">
        <property type="entry name" value="GRB2-associated-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046355">
        <property type="entry name" value="Gab1-4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45960">
        <property type="entry name" value="GRB2-ASSOCIATED-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45960:SF1">
        <property type="entry name" value="GRB2-ASSOCIATED-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="5"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="305"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="488"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="547"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="338"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="553"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="575"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="661" mass="73091" checksum="32048EADB355C2F4" modified="2018-12-05" version="1">MSGGEVIFQGWLRKSPPEKKLRRYAWKKRWFILRSGRMSGDPDVLEYYKNDHSKKPIRVIDLHCCEQVDAGLTFKRKEFQDSFVFDIKTSDRTFYLVAETEDEMNKWVRSICQLCGFNQSDDTHDWTSNLSSCSISDSPDVTGSMAPVSGERKSSAPVHSSQPVLFTFDVPVRHSHHGSLSNSAPQDYLLLHQCMSRKTESARSASFSQSTRSNTFLGSDSPVQKFSYGFSNCLNGTGAQLHGFYSLPKPGKHQLSVHDDSSQEACYVLPRGYSSEAPPHSSLGDPDFENEEVYTFKTPCNTLATMHSNERSPDNYDFPTTPGSFYQIPRTFDKNHNSLTPSSSESSCAPPPRPPKPNQGSEGNWGSPQSAGSQNGEMTSAVSALPRRNTLPAVENIRLHRGRQTDESFTTVRFLSFMSQTPLTRSDSGNSDDNYVPMNPGSSPLSAAQADSPKNVYIPMSPGPHHFDFPGFSATLPARKASSASLCHRPGRLSDVTPPPINRNLKPNRKPKPTPLDLKNNGIIDELPYKSPVTMSWTRPMHEMNASHHCRPISTQSITSTDSGDSEENYVAMQNPASTSPAMSGTSSPAPRKSGNVDYLALDFQPGSPSPHRKPSTSSVTSDEKVDYVQVDKEKTQALQNTMQEWTDVRQSAEPVKGVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8A8</accession>
    <name>A0A3B5L8A8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Serine/threonine-protein kinase</fullName>
            <ecNumber evidence="19">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="19">Activated by DAG and phorbol esters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 19">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8A8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006064.1">
        <property type="protein sequence ID" value="ENSXCOP00000005996.1"/>
        <property type="gene ID" value="ENSXCOG00000004652.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183024"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20839">
        <property type="entry name" value="C1_PKD1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01239">
        <property type="entry name" value="PH_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14082">
        <property type="entry name" value="STKc_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000151">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000056">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000137">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000019">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015727">
        <property type="entry name" value="Protein_Kinase_C_mu-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057764">
        <property type="entry name" value="Ubiquitin_PRKD1-3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968">
        <property type="entry name" value="PROTEIN KINASE C, MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968:SF9">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25525">
        <property type="entry name" value="Ubiquitin_PRKD1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000552">
        <property type="entry name" value="PKC_mu_nu_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 19 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="16 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 19 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="25">
        <location>
            <begin position="89"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="25">
        <location>
            <begin position="214"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="23">
        <location>
            <begin position="364"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="522"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="645"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="528"/>
            <end position="536"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21 22">
        <location>
            <position position="551"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR000552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000552-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000552-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="855" mass="97191" checksum="781B3BEA59605481" modified="2018-12-05" version="1">MAEYCKNIAVTPFPKADKRFLTKNIFSSFCQLPECGFYGMYEKILLFRHDQTSDNMLQLLRSASQIQEGDLVEAILSASATMEDFQIRPHSLFVHSYRAPTFCDHCGEMLWGLVRQGLKCEGCGLNYHKRCAFKIPNNCSGVRRRRNSTVSLTGGIVNVGRPLSAEPSPPQYTDDALLNQLDYYSGRERRASTQSYIGRPIELDKILLSKVKVPHTFLIHSYTRPTVCQHCKKLLKGLFRQGLQCKAQFCFEIVFQSELNSLSFDFNCCVTDLLSPGAESDAVMVEGCLDDPSGDRRGSLLDDMDEALTSDGGLMLDTGSTDLSDLRDPDLDDSNRAISPSTSNNIPLMRIVQSVKHTKRKNSNVMKEGWMVHYTSKDTLRKRHYWRLDSKCITLYQNDTGSKYYREIPLSEILSLEPAQNFSLLPEGANPHCFEITTAMLVYYVGENLLRGEASMTGSSILVSGVGQDVSRMWEMAIQHALMPAVSTGICHNSHRKEISISISVSNSQIQENVDINSVYQIFPDEVLGSGQFGIVYGGKHRKSGRDVAIKIIDKLRFPTKQESQLRNEVAILQSLHHPGVVNLECMFETPERVFVVMEKLHGDMLEMILSSEKGRLPERITKFLVTQILVALRHLHFKNIVHCDLKPENVLLASADSFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLRNKGYNRSLDMWSVGVIIYVSLSGTFPFNEDEDINDQIQNAAFMYPPHPWKKVSQEAIDLINNLLQVKMRKRYSVDKTLSHPWLQDYQMWLDLRNLEGRMNERYITHESDDLRWLHHAQLSGLDYPSNFMNGPLNDIDPGMERYHEQEEELQTLSERVSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8B4</accession>
    <name>A0A3B5L8B4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8B4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007062"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007150.1">
        <property type="protein sequence ID" value="ENSXCOP00000007062.1"/>
        <property type="gene ID" value="ENSXCOG00000005457.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155275"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000012">
        <property type="entry name" value="Voltage-dependent N-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000011">
        <property type="entry name" value="Voltage-dependent N-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF6">
        <property type="entry name" value="VOLTAGE-DEPENDENT N-TYPE CALCIUM CHANNEL SUBUNIT ALPHA-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="312"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="423"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="17">
        <location>
            <begin position="68"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="17">
        <location>
            <begin position="389"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="493" mass="56756" checksum="4F01815EF5DBD8E6" modified="2018-12-05" version="1">MSDIYIFCCFAPVNNIDCGNFQLNEEKKDLEEEEAANGPRQILPYSSMFVFGQTNPVRRLCHYVVNLRYFEMCILIVITMSSITLAAEDPVEANADRNIALKYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFACSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFYCTDESKGLEKDCRGQFLEYDGEEVTAQPREWKKYEFHYDNVLWAFLTLFSVSTGEGWPLVLKHSVDATYEDQGPSPGFRMETSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINARPLTRYMPENKQSFQYKMWKFVVSSPFEYAIMTLIALNTVVLMMKFYKAPTVYESMLKYLNIIFTGLFTLECILKIIAFNPLNYLKEPWNVFDFVTVIGSITDILFTEINVRPAATLCNVTYPLHPTGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8Z1</accession>
    <name>A0A3B5L8Z1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Leucine-rich repeat flightless-interacting protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LRRFIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8Z1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006930"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007018.1">
        <property type="protein sequence ID" value="ENSXCOP00000006930.1"/>
        <property type="gene ID" value="ENSXCOG00000005359.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063564"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019139">
        <property type="entry name" value="LRRFIP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19212">
        <property type="entry name" value="LEUCINE RICH REPEAT IN FLII INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19212:SF6">
        <property type="entry name" value="LEUCINE-RICH REPEAT FLIGHTLESS-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="11"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="102" mass="11985" checksum="C9C93E8D61B0CF1A" modified="2018-12-05" version="1">MHVVLYANRQISEYKFKLSKAEQEMGTMEQNISRLEGQVARYKSAADNSEKVEDELKAEKRKLQRELRTALDKIEEMEMTNSHLVKRLEKMKANRNALLSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9P1</accession>
    <name>A0A3B5L9P1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9P1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006466"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006546.1">
        <property type="protein sequence ID" value="ENSXCOP00000006466.1"/>
        <property type="gene ID" value="ENSXCOG00000005002.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157685"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035613">
        <property type="term" value="F:RNA stem-loop binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1790:FF:000001">
        <property type="entry name" value="roquin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000004">
        <property type="entry name" value="roquin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000047">
        <property type="entry name" value="Roquin-2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041523">
        <property type="entry name" value="ROQ_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048575">
        <property type="entry name" value="Roquin_1_2-like_ROQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052249">
        <property type="entry name" value="Roquin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13139">
        <property type="entry name" value="RING FINGER AND CCCH-TYPE ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13139:SF2">
        <property type="entry name" value="ROQUIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18386">
        <property type="entry name" value="ROQ_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21206">
        <property type="entry name" value="Roquin_1_2-like_ROQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14634">
        <property type="entry name" value="zf-RING_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="13">
        <location>
            <begin position="14"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="408"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="11">
        <location>
            <begin position="408"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="595"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="597"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="615"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1007" mass="112190" checksum="AFFAEBAA82A79ECD" modified="2018-12-05" version="1">MPVQAAQWTEFLSCPICYNEFDSSGHQPISLGCSHTVCKTCLHKLHRKACPFDQTPISTDIDLLPVNCALLQLVGAQVPDVPPVSLSSAAYVEHYDLCRLCVEELALYLKPISGAKGVANLSPSVLSRPMQRKLVTLVNCQLVEEEGRVRAVRAARSLGERTVTELILQHQNPQQLSANLWAAVRARGCQFLGPAMQEDALKLVLLALEDGSALSRKVLVLFVVQKLEARFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRTYEALRREHDAQIVHIAMEAGLRISPEQWSSLLYGDLVHKSHMQSIIDKLQSPESFAKSVQELTIVLQRTGDPANLTSLRPHLELLANIDHNPDAPPPSWAELESVMLAVKLVVHGLVEFIQNFSKKSHETPQANSKYKTSMCRDLRQQGGCPRGTSCTFAHTQDELEKFRLRNKKSGSVVRPFSLASKGLKDAEENMEEASSMTHPLLISRGSGSVEELKRALTNGTSGANGPNRTKSGSTELYEYYSTSFLMNEHASRCGDNLIFIFYVLVFFLIIRKPMSPPRTPVSHSSASSPLSSYYPSALHPPHKSCMARFLRSSNVQESSLPPSSGISSYHNDHQAPHPASSPSSGFPPRDQMGAPTFHPHPGQGQYRHLAPVHGVYAPLYDSRRVWRPQLYHREDARSNSLPPEVLHSSVYQPPLRERFNSLDSNYCSGAEHRAGLHRDYGRVPLGYEDLFRQKQEHWTHIHHHHNPNRPSQSSPIFTTDFGAEHVESSGGQCMGCRFRGEDSLARYSPWSCGSAGPSLSVVSGTGKRWPHSLDHYRRLKEEDPIIPFSEAPIISKWGAISRASRTGYHTTDPVQATACQGSASTTAINFKDYNHHFDHSDYRWSSRGSDSSSHSSFLERYGISTLCQLLFESDPDRDIELELRALDMEDSEHHGIKSQVTKIYSCFKPVIFPCLLLFFNSAFLNLGFSRKSLSPIVLGSGSLGISKLVLQTGPPQLRDSSTRTHPET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAA3</accession>
    <name>A0A3B5LAA3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cerebellin 2b precursor</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAA3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009123"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009234.1">
        <property type="protein sequence ID" value="ENSXCOP00000009123.1"/>
        <property type="gene ID" value="ENSXCOG00000006958.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159988"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099558">
        <property type="term" value="P:maintenance of synapse structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050822">
        <property type="entry name" value="Cerebellin_Synaptic_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923:SF50">
        <property type="entry name" value="CEREBELLIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923">
        <property type="entry name" value="CEREBELLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="C1q" evidence="4">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="113" mass="12993" checksum="FB67C96F80FB7248" modified="2018-12-05" version="1">MEWESNQWVLVNIGNHFDLKASVFQAPKRGIYSFSFHVVKAYNRQTIQVNLMQNDHEVISAFAGDQDVTREAASNGVLLMMERDDRVYLKLERGTLMGGWKFSTFSGFLVFPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LB77</accession>
    <name>A0A3B5LB77_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Neuralized E3 ubiquitin protein ligase 1Aa</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LB77"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009448"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009559.1">
        <property type="protein sequence ID" value="ENSXCOP00000009448.1"/>
        <property type="gene ID" value="ENSXCOG00000007199.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156696"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000022">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEURL1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000056">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase NEURL1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037962">
        <property type="entry name" value="Neuralized"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006573">
        <property type="entry name" value="NHR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12429:SF13">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEURL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12429">
        <property type="entry name" value="NEURALIZED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07177">
        <property type="entry name" value="Neuralized"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00588">
        <property type="entry name" value="NEUZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51065">
        <property type="entry name" value="NHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="NHR" evidence="6">
        <location>
            <begin position="211"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="422"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="475" mass="52941" checksum="90674AF517D05411" modified="2018-12-05" version="1">FTLKQILLPSLPHFVPPHLLTKLFFLFVDSLNGPFPAAPHRFHHKPKRYPPIQPCGNLSSFPLQFHPSTKGSQIIMDLSQRTVKRQASFCNAITFSNRPIAVYEQVRLKQCLWLGARDGYINLFQPIGQVNDSEMVPLDCLRPRSYAATRRSSARRNSHDPRLTISLCDLKDDEGDEEEQLQPLSSNSCHVPQNSQNSQQCLLLPGHLDTDLQFHTIHGLHVTALDKHTVARPDHRGDERTLVFTSRPMHCSEIVFVKVKAGVSRVGSLSYGVTSCDPATLRPSDLPSNPESLVDRKEFWAVCRVAVQLHSGDILSFVVNAEGELMMSHNGSSAGMALCVDNSRPLWMFYGLHGSITQLRILGTLIFPCFDPCHLASCVSISALFLMSLLSSFSDINPSSPFSSHPQSPTFPSSSSSWSDECTICYENMVDTVLYACGHMCLCYACGLKLKKMANACCPICRRTIKDIIKTYRST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBK7</accession>
    <name>A0A3B5LBK7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Rho family GTPase 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBK7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008227"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008325.1">
        <property type="protein sequence ID" value="ENSXCOP00000008227.1"/>
        <property type="gene ID" value="ENSXCOG00000006314.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157020"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000407">
        <property type="entry name" value="Rho-related GTP-binding protein RhoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="191"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="191"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="26202" checksum="F255BB2551378791" modified="2018-12-05" version="1">MESKALLRCKIVVVGDSQCGKTALLHVFAKDSYSENYVPTVFENYTASFEIDKQRIELNMWDTSGSAYYDNVRPLAYPDSDAVLICFDISRPETLDSVVKKWQGEKQEFCPNAKVVLVGCKLDMRTDVSVMRELSKHRLIPVTHEQGTNLARQIGAVAYTECTSKFSENSVRDVFHVTTLASVFNIHRPQVKRATTRRTHKRISQQPPRTEIHEHPPAIRKDRAKSCVLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDA8</accession>
    <name>A0A3B5LDA8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Homeobox protein prophet of Pit-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Pituitary-specific homeodomain factor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Possibly involved in the ontogenesis of pituitary gonadotropes, as well as somatotropes, lactotropes and caudomedial thyrotropes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027861818.1">
        <property type="nucleotide sequence ID" value="XM_028006017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDA8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008530"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008636.1">
        <property type="protein sequence ID" value="ENSXCOP00000008530.1"/>
        <property type="gene ID" value="ENSXCOG00000006529.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114137444"/>
    <dbReference type="CTD" id="5626"/>
    <dbReference type="GeneTree" id="ENSGT00940000165391"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000138">
        <property type="entry name" value="Homeobox protein prophet of Pit-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050649">
        <property type="entry name" value="Paired_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329:SF340">
        <property type="entry name" value="ARISTALESS RELATED HOMEOBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="61"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="63"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="28"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="167"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="224" mass="25156" checksum="2780132382125F35" modified="2018-12-05" version="1">MAHIKAAGATPRSHQDFAELYSDMATVSSSADIGEPGRKAATSETSMAAGISRGGKAYPSPARRRHRTTFSHKQLEQLELAFQQNQYPDIYCREELARITKLNEARVQVWFQNRRAKQRKHERASQKVFPVGVMTGHRTLMAGMPRQYYPQPLAHIPHLSSMLPPGPYSRHQTQVSRCPCPGSPPQPAPQRQHDDWYGSLRTNLPSPMFPLGSMQPLETASPWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDH1</accession>
    <name>A0A3B5LDH1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fibrinogen alpha/beta/gamma chain coiled coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Heterohexamer; disulfide linked. Contains 2 sets of 3 non-identical chains (alpha, beta and gamma). The 2 heterotrimers are in head to head conformation with the N-termini in a small central domain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDH1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010218"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010341.1">
        <property type="protein sequence ID" value="ENSXCOP00000010218.1"/>
        <property type="gene ID" value="ENSXCOG00000007752.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157467"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005577">
        <property type="term" value="C:fibrinogen complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072377">
        <property type="term" value="P:blood coagulation, common pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042730">
        <property type="term" value="P:fibrinolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070527">
        <property type="term" value="P:platelet aggregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034116">
        <property type="term" value="P:positive regulation of heterotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037579">
        <property type="entry name" value="FIB_ANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012290">
        <property type="entry name" value="Fibrinogen_a/b/g_coil_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF6">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08702">
        <property type="entry name" value="Fib_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01212">
        <property type="entry name" value="Fib_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58010">
        <property type="entry name" value="Fibrinogen coiled-coil and central regions"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017199887" description="Fibrinogen alpha/beta/gamma chain coiled coil domain-containing protein" evidence="9">
        <location>
            <begin position="19"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen alpha/beta/gamma chain coiled coil" evidence="10">
        <location>
            <begin position="40"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="401" mass="43735" checksum="97CF9A4D44ED30A1" modified="2018-12-05" version="1">MKKLSLLLCLVSVAITQATLLDPRGARPVEPATRDQKCATQREWPFCTDDDWYSKCPSGCRIQGLMDKNDHELLKKIAKIRSLLDQHKARHRSVDLVSKQTYDILTEKLTMDSGNDNMYYNLAQTLRQRITEMKVRIDRQHKALAALKARVKDQVVEMQKLEIDIDIKLRSCKGSCGAHTVYQVDRDSYVALDKQVNQLDAQVAQSVETVGTLYVMKSRPLQDVIVDSRFKSKDVAGQRTADLFPQVRNALGSPETTGQPSVGVSTKTVSCTKTVRRTVVHTKDGPVEQVEEVGGGPGCDGMGGLTKGGMGSLFPTLTGSSSSSSFSSSSSSLSFHSGGSKGSILADSKSGLDPFGADFGAFMTDNAEDDIPDFHARSVKSTVVERQADFVGTGTKTTELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDI2</accession>
    <name>A0A3B5LDI2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Heme binding protein 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HEBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027896482.1">
        <property type="nucleotide sequence ID" value="XM_028040681.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027896483.1">
        <property type="nucleotide sequence ID" value="XM_028040682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDI2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005924"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005990.1">
        <property type="protein sequence ID" value="ENSXCOP00000005924.1"/>
        <property type="gene ID" value="ENSXCOG00000004589.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114158806"/>
    <dbReference type="KEGG" id="xco:114158806"/>
    <dbReference type="CTD" id="23593"/>
    <dbReference type="GeneTree" id="ENSGT00940000160412"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.80.10:FF:000002">
        <property type="entry name" value="Heme-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.80.10">
        <property type="entry name" value="Regulatory factor, effector binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011256">
        <property type="entry name" value="Reg_factor_effector_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006917">
        <property type="entry name" value="SOUL_heme-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220:SF69">
        <property type="entry name" value="HEME-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11220">
        <property type="entry name" value="HEME-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04832">
        <property type="entry name" value="SOUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55136">
        <property type="entry name" value="Probable bacterial effector-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="192" mass="21967" checksum="5FFB66ED1988F818" modified="2018-12-05" version="1">MLKALGQAFFSTGLKSPKFTAEEAKGQDYEIRTYHPTRWMSTTVSGMQGDECTSTGFRRLFRYIQGNNKNKAKVEMTAPVTCHVVPGAGPACESQFTVSFYIPDELQENPPEPTDPDVFLEDRKEFTAYVRTYGGFSNDNMKREELLKLLESLKRDGVEFVDKPYYTAGYDSPFKLTNRRNEVWVLKKAEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDQ2</accession>
    <name>A0A3B5LDQ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dual specificity protein phosphatase</fullName>
            <ecNumber evidence="13">3.1.3.16</ecNumber>
            <ecNumber evidence="13">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Dual specificity phosphatase able to dephosphorylate phosphotyrosine, phosphoserine and phosphothreonine residues within the same substrate, with a preference for phosphotyrosine as a substrate. Involved in the modulation of AMPK and MAPK1/2 signaling pathways.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Dual specificity phosphatase able to dephosphorylate phosphotyrosine, phosphoserine and phosphothreonine residues, with a preference for phosphotyrosine as a substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 13">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="13"/>
    <dbReference type="EC" id="3.1.3.48" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDQ2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007816"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007914.1">
        <property type="protein sequence ID" value="ENSXCOP00000007816.1"/>
        <property type="gene ID" value="ENSXCOG00000006002.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154628"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033549">
        <property type="term" value="F:MAP kinase phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000037">
        <property type="entry name" value="dual specificity protein phosphatase 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020405">
        <property type="entry name" value="Atypical_DUSP_subfamA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682">
        <property type="entry name" value="AGAP008228-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45682:SF6">
        <property type="entry name" value="DUAL SPECIFICITY PHOSPHATASE 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01909">
        <property type="entry name" value="ADSPHPHTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6 13" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="14">
        <location>
            <begin position="27"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="15">
        <location>
            <begin position="97"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="12">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR620405-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="181" mass="20816" checksum="F0B22F872D71A200" modified="2018-12-05" version="1">ITADDYVTPGTYELEKILNRVSVAYTRVNEVWPNVYIGDEETAMDKCTLKRLGVTHILNAAEGTWNNVDTGPGYYKDMDIVYYGVVAEDVPTFDLSQHFFPATKFIRETLRNPQNKLLVHCVMGRSRSATLFLAYLMIYENMTIVDAIEHVKGHRRIIPNWGFLKQLRELDQQLLEERGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEK5</accession>
    <name>A0A3B5LEK5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">receptor protein serine/threonine kinase</fullName>
            <ecNumber evidence="5">2.7.11.30</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[receptor-protein] + ATP = O-phospho-L-seryl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18673"/>
            <dbReference type="Rhea" id="RHEA-COMP:11022"/>
            <dbReference type="Rhea" id="RHEA-COMP:11023"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[receptor-protein] + ATP = O-phospho-L-threonyl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44880"/>
            <dbReference type="Rhea" id="RHEA-COMP:11024"/>
            <dbReference type="Rhea" id="RHEA-COMP:11025"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LEK5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009000"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009108.1">
        <property type="protein sequence ID" value="ENSXCOP00000009000.1"/>
        <property type="gene ID" value="ENSXCOG00000006866.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157032"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004675">
        <property type="term" value="F:transmembrane receptor protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14143">
        <property type="entry name" value="STKc_TGFbR1_ACVR1b_ACVR1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23536">
        <property type="entry name" value="TFP_LU_ECD_ALK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000045">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000023">
        <property type="entry name" value="Receptor protein serine/threonine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003605">
        <property type="entry name" value="GS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF22">
        <property type="entry name" value="ACTIVIN RECEPTOR TYPE-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08515">
        <property type="entry name" value="TGF_beta_GS"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6 22" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 23" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="99"/>
            <end position="123"/>
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        <location>
            <begin position="150"/>
            <end position="179"/>
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            <end position="470"/>
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        <location>
            <position position="207"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <source>
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    <sequence length="478" mass="53899" checksum="CCBE1E53F3D5BD31" modified="2018-12-05" version="1">LSAALHCNCTNCNGVNSGECETKGACLVSTYKLEGKDVLMRSCLDPDRMIPPIFCKSAEGHLNAHCCFEDYCNNIVLKVPDPTTGEWVTSGSSWGPVELVAVIAGPVFLLCVLLMVGVFLFQYHQRAYSHRQRLEVEDPSCDHLYLAKDKTLQDLIYDMSTSGSGSGLPLFVQRTVARTIVLQEIIGKGRFGEVWRGKWRGGDVAVKIFSSREERSWFREAEIYQTIMLRHENILGFIAADNKDNGTWTQLWLVSDYHEHGSLFDYLNRYSVTIEGMIKLALSAASGLAHLHMEILGTQGKPGIAHRDLKSKNILVKKNGTCAIADLGLAVRHESITDTIDIAPNQRVGTKRYMAPEVLDETINMKHFDSFKCADIYALGLVYWEIARRCNTGGIHEEYQLPYYDLVPSDPSIEEMRKVVCDQKLRPNVPNWWQSYESLRVMGKIMRECWYANGAARLTALRIKKTLSQLSVEEDVKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFD4</accession>
    <name>A0A3B5LFD4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Gamma-aminobutyric acid receptor subunit rho-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">GABA(A) receptor subunit rho-1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">Three rho subunits (rho-1/GBRR1, rho-2/GBRR2 and rho-3/GBRR3) coassemble either to form functional homopentamers or heteropentamers. Rho-1/GBRR1 subunits can also associate with alpha-1/GBRA1 subunits to form a functional GABAAR. Interacts with SQSTM1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRR1 sub-subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFD4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008396"/>
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        <property type="protein sequence ID" value="ENSXCOP00000008396.1"/>
        <property type="gene ID" value="ENSXCOG00000006441.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158591"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19059">
        <property type="entry name" value="LGIC_TM_GABAAR_rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000015">
        <property type="entry name" value="gamma-aminobutyric acid receptor subunit rho-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000005">
        <property type="entry name" value="Putative gamma-aminobutyric acid receptor subunit rho-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0868">Chloride</keyword>
    <keyword evidence="10" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 21" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 21" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 21" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 21" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022254550" description="Gamma-aminobutyric acid receptor subunit rho-1" evidence="21">
        <location>
            <begin position="20"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="444"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="22">
        <location>
            <begin position="66"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="23">
        <location>
            <begin position="273"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00064308"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00070181"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00076172"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008396.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="462" mass="53617" checksum="1DC255239BBAA289" modified="2018-12-05" version="1">MHTDVVLFSVLLWMVEVSGHKRTRGQSLQPYRHIRSFPLFSLSPIFKRSPDITKARATKSEQLLRIDDHDFTMRPGFGGPAVPVGVDVQVESLDAISEVDMDFTMTLYLRHYWKDDRLSFKSNTNQSMTFDGRLVKKIWVPDMFFVHSKKSFTHDTTTDNVMLRVYPDGKVLYSLRVTVTAMCSMDLSRFPLDTQTCSLEIESYAYTDDDLMLYWKEGNRSLNTDERISLSQFLIQEFHTTTKLAFYSSTGWYNRLYINFTLRRHIFFFLLQTYFPATLMVMLSWVSFWIDRRAVPARVPLGITTVLTMSTIITGVNASMPRVSYIKAVDVYLWISFVFVFLSVIEYAAVNYLSTLQERKERKLRDKLPCTCGIAHPGMMSDCYSEVDVNTTGNYGMPEENGMRRERMLVELAMETDQMTGHVGSRAYSSIWIDTHAIDKYSRVIFPGSYILFNIIYWSIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGK2</accession>
    <name>A0A3B5LGK2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Rho GTPase activating protein 42b</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGK2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011263"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011394.1">
        <property type="protein sequence ID" value="ENSXCOP00000011263.1"/>
        <property type="gene ID" value="ENSXCOG00000008508.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155492"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01249">
        <property type="entry name" value="BAR-PH_GRAF_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000114">
        <property type="entry name" value="Rho GTPase activating protein 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000008">
        <property type="entry name" value="Rho GTPase-activating protein 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000074">
        <property type="entry name" value="Rho GTPase-activating protein 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047234">
        <property type="entry name" value="GRAF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047225">
        <property type="entry name" value="PH_GRAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12552">
        <property type="entry name" value="OLIGOPHRENIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12552:SF3">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="7">
        <location>
            <begin position="270"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="6">
        <location>
            <begin position="705"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="466"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="512"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="466"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="513"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="581"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="597"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="763" mass="85791" checksum="AA4FDFD9C7C5E4B3" modified="2018-12-05" version="1">MGLPALEFSDSFLDSPDFRERLKYHEIELERTNKFIKELIKDGNMLITALKSLSAAVQKFSQSLQEFQFECIGDAETDDEVNIAQSFKEFSQLLNTVEEERRRLVQNADDVLITPLEKFRKEQIGAAKVRDPLRIFQGEKLVHQFVLIFEFLWLCSYSRLILPKYAFFSYLTFFLAVNHITVPSLLQFINFLRPMTFVTILLAPFNGFVLNQPETFKLKSCIRRKTDSIDKRFCFDIEVVERHGVMTLQALSESNRRLWLEAMDGKEPIYNLPAILSKKEETQFVYFNELIISFFHFHLGIHTLGLYRIGGVNSKVQRLMSTVFSSKAPVDMELDPEMWDNKTITSGLKNYLRCLSEPLMTFKLHKDFIMAVKSDDQNYRVCAVHALVHKLPERNREMLELLIKHLVIVSAQNQSNLMTVSNLGVIFGPTLMRSQEETVAAMMNIKFQNIVVEILIENFKKIFHQPPDPNVPLPQPQSWTGSRRSRAICLSKGPRKPRSLYTPTLCLADAESDTLSSSPSSTPMGSMESLSSHSSEQNTSSKTASSGAGRCLGRSDLLRQTPSQLSYGPGNAACVSSPESSSREEGGRTDGESEDTFSLSSVSTTPRLSPAPKKAPHCRSDLKPALPSRSVSPSLKSLCSGSVQSLSTLETKESLKPGDHPNLPPKQMIRRRIDASVNNGYQRPGSVVAARALFFENPSSSQSVPIGRDAKAMYSCEAEHSNELSFPQGAHFSNVYPSVEPGWLQATYEGKTGLIPENYVVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIE2</accession>
    <name>A0A3B5LIE2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIE2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011994.1">
        <property type="protein sequence ID" value="ENSXCOP00000011858.1"/>
        <property type="gene ID" value="ENSXCOG00000008972.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00970000193381"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="11"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="6">
        <location>
            <begin position="80"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="160" mass="18502" checksum="2254ADD4FAD69AC6" modified="2018-12-05" version="1">MASRSDDDLCCPVCLEIFKDPVLLSCSHSFCKECLQKYWRDKPGRECPVCKRRSSKDFPPVNLGLKNLCESFLQQREQRASEDLCSLHSEKLKLFCLNHQQPVCLVCRDSEKHTNHRIRPIDEAAQQHKKNLQETLENKLFICSLVSQSPQTLKAHKCSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIV9</accession>
    <name>A0A3B5LIV9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ezrin/radixin/moesin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIV9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010580"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010704.1">
        <property type="protein sequence ID" value="ENSXCOP00000010580.1"/>
        <property type="gene ID" value="ENSXCOG00000008001.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01090000260082"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011174">
        <property type="entry name" value="ERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011259">
        <property type="entry name" value="ERM_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008954">
        <property type="entry name" value="Moesin_tail_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23281">
        <property type="entry name" value="MERLIN/MOESIN/EZRIN/RADIXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00769">
        <property type="entry name" value="ERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48678">
        <property type="entry name" value="Moesin tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ezrin/radixin/moesin C-terminal" evidence="2">
        <location>
            <begin position="40"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="115" mass="13220" checksum="58A00D9BC5AB1298" modified="2018-12-05" version="1">SFSRLCFPPPPAVTFQHDDEHDELNSEAEAELVSDGVSSQRSEEERVTEAEKNDRVKKQLQALSSELAEARDDTKKTQNDMLHAENVRAGRDKYKTLRQIRQGNTKQRIDEFESM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJ03</accession>
    <name>A0A3B5LJ03_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Beta-carotene 15, 15-dioxygenase 2, like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJ03"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007854"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007952.1">
        <property type="protein sequence ID" value="ENSXCOP00000007854.1"/>
        <property type="gene ID" value="ENSXCOG00000006031.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182913"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003834">
        <property type="term" value="F:beta-carotene 15,15'-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010436">
        <property type="term" value="F:carotenoid dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016121">
        <property type="term" value="P:carotene catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042574">
        <property type="term" value="P:retinal metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF148">
        <property type="entry name" value="BETA-CAROTENE OXYGENASE 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16662" checksum="04256104E1D50230" modified="2018-12-05" version="1">TGCYRQRTLTSAQKNPKHFTDVHGLPCIEKIVTSVEDIPEPISTNIIGMIPNWINGNFLRNGPGKFEMGNHRFNHWFDGMALLHQFKISNGEVTYRSRFLSSDSYQTNKEQNRIVMSEFGTLTMPDPCKNFFQRFVSRFELPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJE4</accession>
    <name>A0A3B5LJE4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027875342.1">
        <property type="nucleotide sequence ID" value="XM_028019541.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027875343.1">
        <property type="nucleotide sequence ID" value="XM_028019542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJE4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010780"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010905.1">
        <property type="protein sequence ID" value="ENSXCOP00000010780.1"/>
        <property type="gene ID" value="ENSXCOG00000008136.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114145888"/>
    <dbReference type="KEGG" id="xco:114145888"/>
    <dbReference type="GeneTree" id="ENSGT00940000161259"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043549">
        <property type="entry name" value="C2C4C/C2C4D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46291:SF11">
        <property type="entry name" value="C2 CALCIUM-DEPENDENT DOMAIN-CONTAINING PROTEIN 4C-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46291">
        <property type="entry name" value="C2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="2">
        <location>
            <begin position="311"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="174"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="427" mass="47019" checksum="504D542B04819714" modified="2018-12-05" version="1">MWLLEKLRSSVESSGTHSQPPQTTEAIPVAVYANVLTPERIPDFFIPPKLICCPPEESTSPEPKPRTNLRPSSSDNAICSQSPRARSSKSICSPRLFCRNLQKSANRHIIQIETADEPGTGTMDKHTVNINTNADPQSQTAMSLPYVPKAQTSYGFSTLVESPHTRRKESLFHSDPNSPLNSPSSQRRSQGGTLLAPADPNPYRYFSGGESDTCSSAESSPFNSPLLSRSASLLRSITQETQAKVSRARRSLARHSSLSTDECSSADNSPNMQRRRARCPPSPAFRGAKAGSSRPAASDLLQREHTINLHKGGTLKLSTHYDPEAARLRVRVLAAEALYNRQTDLKSINCCVALYLNPGKQQKQRSTIIKNSRNPVFNEDFFFDALPQAQVKNLAMKIKVVNKGTSLKRDVLLGEREVLLSELLANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKL8</accession>
    <name>A0A3B5LKL8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">IgLON family member 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the immunoglobulin superfamily. IgLON family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKL8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010136"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010255.1">
        <property type="protein sequence ID" value="ENSXCOP00000010136.1"/>
        <property type="gene ID" value="ENSXCOG00000007689.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160467"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000013">
        <property type="entry name" value="cell adhesion molecule 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000305">
        <property type="entry name" value="neurotrimin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050876">
        <property type="entry name" value="IgLON_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42757:SF12">
        <property type="entry name" value="IGLON FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42757">
        <property type="entry name" value="IGLON FAMILY OF IMMUNOGLOBULIN SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="41"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="128"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="224"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="347" mass="38651" checksum="0AAD897F15FDA275" modified="2018-12-05" version="1">MKGIFSDFDVTVDSFTCGMSLLDAHCTLLSFLLAGSNVLGAEFSHLPDNITVLEGESVTLRCRIDEEVTHKAWLNRSNILFTGTDKWSLDKRVTLVNTSNREFSILIDKVQVTDEGPYTCTFQANNKPRITHVYLIVQVPARIVNISKSVSVNEGENVNLYCLAVGRPEPTVTWKDQKYGLVSEGEFLDITEIKRQQAEDYECITHNGVAPPDQQKVRVTVNYPPMITDMKNMPANLGKTAILRCDAMAVPPATFEWYRDDHRPVENDNTLRIKNEKTRSLLLFTNVTEKHFGNYTCFASNRLGSSNASMLLFRPGALQGRGAGLHAGMSASLCVWVSLSAVLLVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LM58</accession>
    <name>A0A3B5LM58_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Translocated promoter region a, nuclear basket protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000010606"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010730.1">
        <property type="protein sequence ID" value="ENSXCOP00000010606.1"/>
        <property type="gene ID" value="ENSXCOG00000008018.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111014"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901673">
        <property type="term" value="P:regulation of mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012929">
        <property type="entry name" value="Nucleoprot-TPR/MLP1-2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057577">
        <property type="entry name" value="Nucleoprot-TPR/MLP1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18898:SF3">
        <property type="entry name" value="NUCLEOPROTEIN TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18898">
        <property type="entry name" value="NUCLEOPROTEIN TPR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25481">
        <property type="entry name" value="Nucleoprot-TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07926">
        <property type="entry name" value="TPR_MLP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoprotein TPR/MPL1" evidence="4">
        <location>
            <begin position="163"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoprotein TPR/MLP1-2" evidence="3">
        <location>
            <begin position="944"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="483"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1071"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1359"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1492"/>
            <end position="1666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1702"/>
            <end position="1770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1788"/>
            <end position="1819"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="22"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="418"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="513"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="645"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="493"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1077"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1376"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1492"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1519"/>
            <end position="1564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1583"/>
            <end position="1595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1603"/>
            <end position="1614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1615"/>
            <end position="1647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1654"/>
            <end position="1665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1708"/>
            <end position="1721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1734"/>
            <end position="1745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1746"/>
            <end position="1761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1807"/>
            <end position="1819"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1978" mass="224695" checksum="479A19200A1F27CC" modified="2018-12-05" version="1">MAVVLLQALERTELNKLPKGVQNKLEKFVADLQNANEALKTQHERFKADSEQRYFDFEKQLTESQEQILSATNDLQILKEENKKLSEFLFILLHLCIFFNLSLQQTKAKYEIEAEKRELERLLEKRTQEVENLTEDMKRLNEKLTDTNKLKMELQLKLDEIQSSTASVQHREKRMEQEKELLEKKVDWLTAELSTKTEELLNTNREKGKEILELQGSLQSSNEQVSRLESQLTSLKELSESQSKRAEDLNNKLKQAKDEQVAMEEKYRVELNAHVKLSSLYKGAATDMEAKNQELSRAVEELSKLIKETGEANKGLEKKVSDAEEQKVRVEAELREKIKKMEKELENATMKAAGKNCGPSLTEEQLDSMCPSATAIAAIVKPGMKFFDLYNAYAECQTHLQLEKQETRRVSKVLDEIVQEVESKAPVLKRQREEYESMQRSMASLCNKLEQARTVSNPDLEFNVLTCLAQLCNLLVELEEARGNRVSKDDGSSSDISSTSEVNSSSQLSFRSVMELQRQNQSLLGRLRELEEEKSRERITELESVVDKLQKEVEQLREQRNQQKQLADSNARQRDMFKALLTQSTGFSLPPQGQELILRPSAPATRSTPQRAAAAESSQTIHTKAALKQLNDSFALYKKEKAENDRMLNETNDRLQKQLAELRSSHAKLTSQLDFSNKRYEILQETVSAYRREINALQKSNQTMAATVQRHEHNIHTMVQDLRKANEKLALEQVRVENLTKERDMLRQAECRLDKEKEAVLAEQRNQNLLLTNLKTIQLAMERTETETRQRLNNKIEQLEAELASIKTKLEEEVAQRHTLGRTMDAQLLEAKKQLETQNILLQKTKELLRGSEQQVTALKAQLASASSSEAPGQSEQELSDLKAQLRSAEEQNAELAEQLKNANATVEQYRSVVLTLEDSLKKEKESRSPLEVRLKQSEDVQKQLEKKMLEVEKLKQQELEERKKAVDAVEKQVHFKETTGEAQAKYERELMLHAADVEALQQLKKTSQHEAARKREIEEELKKATSLLQVKTNVWITTEKRLKEELSSLKSRFEELGKQNSLLLQQMDEMAARSRQQQQQQQQQQQQQLDLSFSEDGKTTEQILEILRFVRREKEIAVAQSEASEGEALRYKQQVEHKDRELKELQEALNAERERMQLTSKTLCEQQEQLKKMESISALQENNRMLKLDRDKLELELQQAQAKVTKLQSDIAPLHNSLSILSEKNGSLQADKRLLEDDLKRWKAKVQHLVSQQKEGDAEEKQKITAEKEAQQKRIAQLIEEAAKLRTELARSNASSNSAQAQLQGLKDSVVRLTSEKDSFKKDLETKNNEILEKNRTITQVKKIGRRYKTQYEELKAQNDKVRQSTFKSQQDLEAAQKEHQQSKEKLQELQTQLTQSQNELTQVTHTDIKIKNREDQLKLQMAEKEEKTKKAILGAKLKIGQVNSAKEQLSQENEELKQSKEELEVRMNALKSQYEGRLLRMDRELRELRETQAHSDGREETQDQGVAKLDQPRSSDQRQISLKSPAQDRGSLSTSEPPTANIRPTPSTQSPSNKPSPSTGSKATPRASIRPMVTPAPVPNQTPTATVMPTTQTESQEVLISTGASVHSTSSSLMNPLTSLTQPTSTQATAFVQPTQQQAANQDAGSSIEADCPSTSSSCMPSTGDQQHRLQIYIYTVQMCFNLSVFALQELPEEGFQVFSEEDEGVSQSVPSDQGSQGSALIRDVIVIDTDSESRESKEGDQRVEEEDEDEEEDEEEEERSTTRRVRRLKMRERKPALLTPHRAVSPKDYLHHPPTCSTAHSPCSSSLMPRLSQPRRPSPRLFIQAPAPELGPPHTQMLMSAENVCVFLQPFFDDDDRMVPSTPTLVVPHRTDGFAEAIHFTFGPGTADIAFVHVGLGMYESPLFLAAHDEDGGGRSVPTTPLQVAAPVTVFTENMPSDSGDNLASQSVPMVTASTGMAAAADDGDEVFMEHEGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMJ9</accession>
    <name>A0A3B5LMJ9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMJ9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012337.1">
        <property type="protein sequence ID" value="ENSXCOP00000012192.1"/>
        <property type="gene ID" value="ENSXCOG00000009230.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="67" mass="7334" checksum="FFC1338FD6D3D1A2" modified="2018-12-05" version="1">ITQFVLQHDLFLLLSIQVEEGAISFHSLFVAACVSACVLSVGVQQHRSCLTACMVLQAPLCLYEIPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMY5</accession>
    <name>A0A3B5LMY5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL37</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L37, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the mitochondrion-specific ribosomal protein mL37 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMY5"/>
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    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
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        <source>
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            <dbReference type="ARBA" id="ARBA00023274"/>
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            <dbReference type="ARBA" id="ARBA00037985"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00039442"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00041617"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="429" mass="48787" checksum="F827D44917074204" modified="2018-12-05" version="1">MIPKATVSLFSPNIFLNASKLSAHGALSPQTRRNLRVSHCLAAKVPPPKKPREKVEIPGLEMVTYGDRMHYVPGLAKPVYPPWEMSFKDPRYYRSPPAQEMALYKDKPCYVFNQRTSILEGVRQAAWLTKTHVNPGLLPHLISLAAKPENQLPDQDERVQNAIKHARFWDTTEPRPRKEKYSNALLLNLLHLCASLQPTHPAVGRRILAENYSLAATWKRGEDLFQIRGQNGLLHCSMDPLPQICGEQEVLETSDRVLESFYPVSPTIDLQQVSVYKEEMNYTGFRGEYPYPHAHTLYFLETSDPNCQLRPEQFRAKMIMFTFGNALARAHKLYGVRPHSLLECPITVQAVGTNGRIFQFLVFQLNTTDLSGDDGIKNQAWLDEDVELYDFAKVRPFIKRKEVKVPAGLAGYKPETFGKFLALYLHGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LP39</accession>
    <name>A0A3B5LP39_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">SATB homeobox 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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        </subcellularLocation>
    </comment>
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        <property type="entry name" value="DNA-binding protein SATB"/>
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        <property type="entry name" value="DNA-binding protein SATB"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
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        <location>
            <begin position="72"/>
            <end position="173"/>
        </location>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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            <end position="321"/>
        </location>
    </feature>
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        <location>
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            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="263"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="291"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="472"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="Proteomes" id="UP000261380"/>
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    </evidence>
    <sequence length="679" mass="75652" checksum="57A34586CC50873F" modified="2018-12-05" version="1">MDHLSESCLGKENSELLVGGLAEAKAPPAKLPRLEQNGSPLGRARLGSAGAKLAGIPYKPAGHLLKACHKRGNMLPVFCVVEHYENPMDFDSKEEHAEFVLLRKDMLFNQLIEMALLSLGYSHSSAAQAKGMIQVGKWNPVPLSYVTDAPDATVADMLQDVYHVVTLKIQLHSCPKLEDLPPEQWSHSTVRNALKELLKDMNQSSLAKECPLSQSMISSIVNSTYYANVSAAKCQEFGRWYKHFKKTKDMMAEMDSLSDLSPPGSNHNHNHLGFSQQQPIPGSTAEQTPSSPVPPLPHAPPHGGQTGGRQPQLPGLHHPGLVSTPISPQLVNQQLVMAQILNQQYAVNRLLAQQSLSQQYLNHPPVNRNSHTKPMEPQVGSNTEVSSEIYQWVRDELKRAGISQAVFARVAFNRTQGLLSEILRKEEDPKTASQSLLVNLRAMQNFLQLPEAERDRIYQEERERSLTAASAMGSTPLISTPTSRPIQVKPSPLPSEWNGKTENCILNITSTIYDEIQQEMKRAKVSQALFAKVAASKSQGWLCELLRWKEDPSPENRTLWENLSMIRRFLSLSQAERDAIYEQESSAGQQHHNERPPHLIHVSTDQLQVNSTHKTEVFNYFLRGPVPLGPWTGTGPRPGGRGRLLYIIKTQKSTTETLNFSNCRHLQIKMLVSVSLVMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPN0</accession>
    <name>A0A3B5LPN0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histone-arginine methyltransferase CARM1</fullName>
            <ecNumber evidence="4">2.1.1.319</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Coactivator-associated arginine methyltransferase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Protein arginine N-methyltransferase 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Atherinomorphae</taxon>
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            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11991"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
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            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
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        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
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        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0035242">
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:regulation of skeletal muscle fiber development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH_CARM1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Coactivator-associated arginine methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Probable histone-arginine methyltransferase CARM1"/>
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    </dbReference>
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        <property type="entry name" value="Putative Histone-arginine methyltransferase CARM1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
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        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF51">
        <property type="entry name" value="HISTONE-ARGININE METHYLTRANSFERASE CARM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11531">
        <property type="entry name" value="CARM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 18" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 18" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="19">
        <location>
            <begin position="289"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="605" mass="66471" checksum="87E221A5BFE22EDC" modified="2018-12-05" version="1">GRCSARAYLPGVGPSGSSTECGEQAMAVSVFRGVRLLSIGDANGDIQRHSEQQPLRLEVKTSQDAALINLSNEETSVFKCSVSRDTECSRVGKQSFIITLGCNSVLLQFSSPADFQSFYNLLKNCRGHAGEHSVFSERTEESSAVQYFQFYGYLSQQQNMMQDYVRTGTYQRAILQNHTDFKDKVVLDVGCGSGILSFFAAQAGARKVYAVEASTMAQHAEVLVNSNRLSERVVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKFLKPSGKMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAVDEYFRQPIVDTFDIRILMAKSVKYTVNFLEAKEEDLYRIEIPFKFHMMHSGLVHGLAFWFDVAFMGSVMTVWLSTAPTEPLTHWYQVRCLLQSPLFAKAGDTLSGTAMLVANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPNPPPGSHYTSPSENMWNTGATYSMSQGMAGSGMPTAYDLSTVIGSGPAVSHNNLIPLVNTGIVNHTHSRMGSIMSTGIVQGATTGQSGPSSSGTYYPITNQFTMGGAAISMASPMVIPSNTMHYGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQF6</accession>
    <name>A0A3B5LQF6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphatase and actin regulator</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="5">Binds PPP1CA and actin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the phosphatase and actin regulator family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQF6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013441.1">
        <property type="protein sequence ID" value="ENSXCOP00000013277.1"/>
        <property type="gene ID" value="ENSXCOG00000010050.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157562"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2130">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004018">
        <property type="entry name" value="RPEL_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12751:SF7">
        <property type="entry name" value="PHOSPHATASE AND ACTIN REGULATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12751">
        <property type="entry name" value="PHOSPHATASE AND ACTIN REGULATOR PHACTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02755">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00707">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51073">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RPEL" evidence="4">
        <location>
            <begin position="349"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="repeat" description="RPEL" evidence="4">
        <location>
            <begin position="387"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="RPEL" evidence="4">
        <location>
            <begin position="425"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="67"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="220"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="271"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="398"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="74"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="164"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="271"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU301113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="507" mass="57596" checksum="5DC17C2A204447A4" modified="2018-12-05" version="1">LFPCRFPKHSIVSGSLAQEDPLLSVVDVMDQQRALHSDCLVSGIRTPPVRRNSKLASLGRIFKPWKWRKKKSEKPRPPGDELFKRSPGEMETDSDLACGGNGEDPDTPTQSDGEDRDEDLMAPLASASEDMGSDSEDNFVAAGEINRGEDEDAEDGNVSLSDSSEERTMETAEAAEAKQEMVAAKPQRRASTPPPPSLPVKLLTRLGSLDVSVKKSPATLPRNFTLPKEPHGSLLRGRISTPTGSPHLGALHQPLPPSCIIEELHRALATKHRQESFQTKEARCSPKRRADGRLSRTSSMERESLGEMDGKKRSDENKENCSLDQYASDQDSWNESVISGTLPRRMRKELLAVKLRNRPSKQELEDRNIFPVRSDQERQEIRQQIEMKLAKRLSQRPAVEELESRNILKQRNDQIEQEERREIKQRLNRKLNQRPTVDELRDRKILIRFSDYVEVAKAQDYDRRADKPWTRLSAADKAAIRKELNEFKSNEMEVHSSSKHLTRFHRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQV9</accession>
    <name>A0A3B5LQV9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase</fullName>
            <ecNumber evidence="15">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + H2O = 1D-myo-inositol 1-phosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58433"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:43485"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:33180"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="17">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQV9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010169"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010288.1">
        <property type="protein sequence ID" value="ENSXCOP00000010169.1"/>
        <property type="gene ID" value="ENSXCOG00000007714.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155428"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120548">
        <property type="term" value="F:phosphatidylinositol phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16208">
        <property type="entry name" value="EFh_PI-PLCbeta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08591">
        <property type="entry name" value="PI-PLCc_beta"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.10.238.10:FF:000048">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
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        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1230.10">
        <property type="entry name" value="Phospholipase C beta, distal C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
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        <property type="entry name" value="C2_domain_sf"/>
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        <property type="entry name" value="PI-PLC_fam"/>
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        <property type="entry name" value="PLC-beta"/>
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        <property type="entry name" value="PLC-beta1_EF"/>
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        <property type="entry name" value="PLC-beta_PH"/>
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        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
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        <property type="entry name" value="PLCB1-4-like_EFh"/>
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        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
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        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
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        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE BETA-1"/>
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        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PHPHLIPASEC"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C-terminal domain of PLC-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 17" id="KW-0106">Calcium</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 15 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 15 18" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="9 15 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PI-PLC Y-box" evidence="22">
        <location>
            <begin position="554"/>
            <end position="670"/>
        </location>
    </feature>
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        <location>
            <begin position="670"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
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            <begin position="451"/>
            <end position="548"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="20">
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="20">
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        <location>
            <begin position="1141"/>
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        </location>
    </feature>
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        <location>
            <begin position="928"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="458"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="475"/>
            <end position="490"/>
        </location>
    </feature>
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            <end position="532"/>
        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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        <ligand>
            <name>Ca(2+)</name>
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        </ligand>
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        <location>
            <position position="346"/>
        </location>
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    </evidence>
    <sequence length="1180" mass="134343" checksum="7CA432F973CC42C7" modified="2018-12-05" version="1">MRGKRENHDYLIDLEVWNPTKKKRHYKITLVHAPKYYIYLSDFHYTHKKSFVIIFFSASLCVSSPKDAKLRELLDVSTLAGKLENRMLTVVSGTDMVNITYLNFMAFQEEIAKEWAEELFSLASNLLAQNMSREACLEKAYTRLKLQLNPEGRIPVKNIFRMFSADRKRVETALENCNLPSGRNDSVPQEDFTPEVYSMFLSNICSRPELDNIFSEVGAKSRPYLTVEQMTEFINSKQRDPRLNEILYPPLKPEQVQLLVDKYEPNMSLAQKGQISMEGFARYLNGEENSIIPPEKLDQSEDMTFPLSQYFINSSHNTYLTAGQLAGNSSVEMYKQVLLSGCRCIELDCWKGRTTEEEPVITHGFTMTTEISFKEVIEAIADCAFKTSPFPVILSFENHVDSPKQQAKMAEYCRSIFGDALLMEPLEKYPLDAGVPLPSPMELMGKILVKNKKKHHKTEGSTKKKLSEQVSNTCSDSSSVCEPSSPSAGSTKEEMADSSQPSDGAELNLKPHGRKSIGEVEAESDDEDDDDDDCKKGSMDEGTAGSEAFATEEMSNLVIYIQPVKFYSFEMSKKINRSFQMSSFVETKALEQLTKSPVEFVEYNKLQLSRIYPKGTRVDSSNYNPQLFWNAGCQLVALNFQTIDLSMQLNLGMYEYNGKCGYRLKPEFMRRPDKHFDPFAESTVDGIVANTLSVKIISGQFLTDKKVGTYVEIDMFGLPVDTKRKAFKTKTSQGNAINPLWEEEAIVFKKVVLPTLASLRIAVFEEGGKFIGHRIMPVSAIRPGYRYINLRNEKNQSLMLPALFVYVEVKDYVPDTFADVIEALSNPIRYVNLMEQRANQLAALTLEEGGEEEGDKEVEAGSDAPSESKVDQKAAVPSENGVSHTPIIAPKPPSLVGHQPQSAGSLKPSAKTEDIIQSVLTELEAQTVEELKQQKGFVREQRKQYKEMKELVRKHHRKTSELIKEQTARVSELQSQHQRRRSTLQKSHKRDCKKSRSENSLSTLDEELCELDQECGQKLAELKEQQQQQLLKLRQEQYYSEKYLKREHIKQLVEKLTTIAEECQSIKYLKLCVCILQLEDAQCKRQERLQEKHKDTRQQILDEKPKLQSDLDQEYQDKFRRLPVEIQEFVQDSSKTKLCDDSLPGNLISSSTTERLNHKASQSEDDMEEGSSEHAFDTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRJ7</accession>
    <name>A0A3B5LRJ7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zinc finger protein 330</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Nucleolar autoantigen 36</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOA36 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRJ7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014869.1">
        <property type="protein sequence ID" value="ENSXCOP00000014683.1"/>
        <property type="gene ID" value="ENSXCOG00000011146.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017043"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010531">
        <property type="entry name" value="NOA36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13214">
        <property type="entry name" value="ZINC FINGER PROTEIN 330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13214:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06524">
        <property type="entry name" value="NOA36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="260"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="269"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="317" mass="35524" checksum="F988F3AE0E622513" modified="2018-12-05" version="1">MPKKKTGARKKAENRKEREKQIRANREHVEVAKHPCNVNMECDKCQRKQKNRAFCYFCNSVQKLPVCAQCGKTKCMKASDCVIKHPGIHSTGMAMVGAVCDFCEAWVCHGRKCLSTHACSCPLADADCIECERGVWDHGGRIFRCSFCRNFLCEDDQFEHQASCQILQAETFKCVSCNRLGQHSCLRCKACYCDDHAKSKVFKQEKGKAPPCPKCGHETQETKDLSMSTRTLKFGRQAGGDDDDDYEGASGYDAYWKNLAAGGSRQDDYGDDDDDGDEEEEDDGYEEDDEEEEDEGEEEDETASESLAELKIDGTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTI5</accession>
    <name>A0A3B5LTI5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTI5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015318"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015509.1">
        <property type="protein sequence ID" value="ENSXCOP00000015318.1"/>
        <property type="gene ID" value="ENSXCOG00000011608.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160081"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032183">
        <property type="term" value="F:SUMO binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18627">
        <property type="entry name" value="CD_polycomb_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000006">
        <property type="entry name" value="Chromobox protein homolog 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043531">
        <property type="entry name" value="CBX4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017984">
        <property type="entry name" value="Chromo_dom_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46727">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE CBX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46727:SF1">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE CBX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00504">
        <property type="entry name" value="CHROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="6">
        <location>
            <begin position="11"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="60" mass="7201" checksum="C9B019E99C749D14" modified="2018-12-05" version="1">MELPAAGEHVFAVESIEKKRSRKGRVEYLVKWRGWSPRYNTWEPEENILDPRLLDAFQDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LU06</accession>
    <name>A0A3B5LU06_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Scavenger receptor class B, member 2a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD36 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027888519.1">
        <property type="nucleotide sequence ID" value="XM_028032718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LU06"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012801"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012955.1">
        <property type="protein sequence ID" value="ENSXCOP00000012801.1"/>
        <property type="gene ID" value="ENSXCOG00000009672.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114153998"/>
    <dbReference type="KEGG" id="xco:114153998"/>
    <dbReference type="CTD" id="192340"/>
    <dbReference type="GeneTree" id="ENSGT00940000153372"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006622">
        <property type="term" value="P:protein targeting to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002159">
        <property type="entry name" value="CD36_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005429">
        <property type="entry name" value="LimpII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923:SF56">
        <property type="entry name" value="LYSOSOME MEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS B TYPE-1 SR-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01130">
        <property type="entry name" value="CD36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01609">
        <property type="entry name" value="CD36FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01611">
        <property type="entry name" value="LIMPII"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="435"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="494"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="530" mass="60019" checksum="FA01FE04CBCFCBA0" modified="2018-12-05" version="1">MTRRSCAIYATGIVCAHLLIVGIALVVAQVFQTMINSRLKKEITLTEKSQMFESWKNPPPPVYMEYYFFNVTNPEEFLAGGKASVKQIGPYTYREYRPRENVTFLENSTKVYALNPKSFVFVPEKSAGDPEVDIIRTVNIPYVAVMNEMNSYSFYVRPMVSMYVGSLGVEIFMTRTVHEILWGFKDPLLSKIHRLKREVDEYFGLMWKKNGTHEGEFVFHTGEQNYLDYGKIDTWNGLRKMTWWSSNQSNMINGTDGAVFHPLIDRNELLYIFAADLCRSIHLAYVEDVEVKGIQAYRFAPPNDVLMNPKENPTNAGFCVPAGECLGTGVLKVSVCREGAPIVVSFPHFYQADPAYINAVGGMNPNKEEHETYLDLQPTTGVPIRACKRAQLNIILKRVPGFPKTKFINETIFPIMFVNETATIDDDSASQMRTLLLILTLVSNFPLIIVGLGIILLLVLVVLFCRNRQKKNEVKRIDFTEAFHSFTTAKDDTAYTQVSDKPDDSSEKPANQPMKNGSYIAMSPVEAQKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWD0</accession>
    <name>A0A3B5LWD0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Interferon regulatory factor 8</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWD0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015437"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015629.1">
        <property type="protein sequence ID" value="ENSXCOP00000015437.1"/>
        <property type="gene ID" value="ENSXCOG00000011679.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158140"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014005">
        <property type="term" value="P:microglia development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045649">
        <property type="term" value="P:regulation of macrophage differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045658">
        <property type="term" value="P:regulation of neutrophil differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00103">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000041">
        <property type="entry name" value="Interferon regulatory factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.10:FF:000013">
        <property type="entry name" value="Interferon regulatory factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019817">
        <property type="entry name" value="Interferon_reg_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001346">
        <property type="entry name" value="Interferon_reg_fact_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019471">
        <property type="entry name" value="Interferon_reg_factor-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11949">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11949:SF7">
        <property type="entry name" value="INTERFERON REGULATORY FACTOR 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00605">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10401">
        <property type="entry name" value="IRF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00267">
        <property type="entry name" value="INTFRNREGFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00348">
        <property type="entry name" value="IRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01243">
        <property type="entry name" value="IRF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00601">
        <property type="entry name" value="IRF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51507">
        <property type="entry name" value="IRF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="IRF tryptophan pentad repeat" evidence="7">
        <location>
            <begin position="6"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="411" mass="46956" checksum="857A1D32F167495B" modified="2018-12-05" version="1">MSNSGGRRLKQWLIEQIQSAQYSGLQWEDESRTMFRIPWKHAGKQDYNQEVDASIFKAWAVFKGKFKEGDKAEPATWKTRLRCALNKSPDFEEVTERSQLDISEPYKVYRIVPEEEQKHGKIPVMTMTGTASSGDHTDMACSPTEIEELIKEEDSCSIQASPEYWPQSSMNGKKKNCFSQLMISFYYGGKLMYNTLVTHPEGCRISPQQHLGRGAPYSSDSMQNIHFPPAELIEYERQRYFTRKLLGHLERGVLVRANQEGIFIKRLCQSRVFCSGLGDGSSQYGSMPWKLERDSVVKIFDTGRFFQALQLYQEGQIAAPDPSVTLCFGEELNDVSNAKSKLIVLQITMVNCQHMLESLNLRRSQPYTSSPNVEMSDDVATEQMARIYQDLCSYSVPQRPACYRDNMPITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWD1</accession>
    <name>A0A3B5LWD1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Si:ch211-129c21.1</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWD1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012149"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012288.1">
        <property type="protein sequence ID" value="ENSXCOP00000012149.1"/>
        <property type="gene ID" value="ENSXCOG00000009187.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165728"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043931">
        <property type="term" value="P:ossification involved in bone maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001703">
        <property type="entry name" value="Semaphorin 4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF135">
        <property type="entry name" value="SEMAPHORIN 4D ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017395670" evidence="9">
        <location>
            <begin position="25"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="691"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="11">
        <location>
            <begin position="25"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="555"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="797" mass="89338" checksum="BCA376E93E51255B" modified="2018-12-05" version="1">MSLLLTLSIVCGLMLQHYFSLLSAQYCVPRKTVNYQSHLNLFKEDGIFNYSTMLIRDDLGVVLLGAREAIYALDINNISDQKAAVYWRVTEEKQRECTYKGKHAEVECRNYIRTLHRVNDTTLYVCGTNAFSPVCDYLTFANGQLTLKGKQEEGKGKCPFDPFQRYSSLMVGTELYSATSINFLGSEPVILRSSNSPLRTEFKSSWLSEPNFVYMDFLEESFDSPDGDDDKVYLFFSENAMEYDFYRKVTVSRVARVCKGDMGGLRTLQKKWTSFLKARLDCSFPEPSLPPVVQDVFLLRHKDWRKSVFYTVFTPQSSLSQISAVCAFTVASIREIFNEGKFKTTVAVETSHVKWVMYTGEVPEPRPGACINSIDRKKGMNRSLDLPDKTLQFIRDRPLMDEAVRPLTGGPLLLKRGPLLTRIVVDSVLALDGDTYNVMFIGTENGYIQKAVNFDGEMFIIEQIQVYENPEAVKVLRLSSSKGQLYAGSESGVIQMPVSNCSRYQSCQDCILSRDPYCAWDSSAERCSSISSLSASLEAAVQSLKEGNVSQCPPPGASIMTPASFMRNNIQLPCQRHSNLAEVHWRFSDLQVSSTHKYYIYSDGLLILNTTASDAGSYFCDSVEQINSRMYNRTVAVYHLQISSEPVSPTPGTAATKYFIQTLKTAATGSQPDLPTPENQSDSGRMSRLEVAVALLSLLCLSLVAVILWFWKRGHWNCFGLAQSSKGGEGKRPLSGYTHDQNRNSENKLLGAEVKPCTANNNHTTVDFKKNGEYHFPPLANISNLNGLGYIHDESEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LX19</accession>
    <name>A0A3B5LX19_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">V-type proton ATPase subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9 11">Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex. The V1 complex consists of three catalytic AB heterodimers that form a heterohexamer, three peripheral stalks each consisting of EG heterodimers, one central rotor including subunits D and F, and the regulatory subunits C and H. The proton translocation complex V0 consists of the proton transport subunit a, a ring of proteolipid subunits c9c'', rotary subunit d, subunits e and f, and the accessory subunits ATP6AP1/Ac45 and ATP6AP2/PRR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the V-ATPase e1/e2 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027873778.1">
        <property type="nucleotide sequence ID" value="XM_028017977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LX19"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015692"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015886.1">
        <property type="protein sequence ID" value="ENSXCOP00000015692.1"/>
        <property type="gene ID" value="ENSXCOG00000011856.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114144796"/>
    <dbReference type="KEGG" id="xco:114144796"/>
    <dbReference type="CTD" id="553767"/>
    <dbReference type="GeneTree" id="ENSGT00940000162476"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008389">
        <property type="entry name" value="ATPase_V0-cplx_e1/e2_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017385">
        <property type="entry name" value="ATPase_V0-cplx_e1/e2_su_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12263:SF2">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT E 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12263">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05493">
        <property type="entry name" value="ATP_synt_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038097">
        <property type="entry name" value="V-ATP_synth_e1/e2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR038097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="81" mass="9101" checksum="6B0F4F528497A504" modified="2018-12-05" version="1">MASHSLALPMICFTTLWALVGVVAPFLVPKGPNRGVIVTSLILTAVCCYLFWLIAILAQANPLFGPQLKNETIWYLQYYWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M053</accession>
    <name>A0A3B5M053_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Fignl1 interacting regulator of recombination and mitosis</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M053"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013374"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013539.1">
        <property type="protein sequence ID" value="ENSXCOP00000013374.1"/>
        <property type="gene ID" value="ENSXCOG00000010130.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004791"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027902">
        <property type="entry name" value="DUF4487"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16071">
        <property type="entry name" value="CHROMOSOME 1 OPEN READING FRAME 112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16071:SF2">
        <property type="entry name" value="FIGNL1-INTERACTING REGULATOR OF RECOMBINATION AND MITOSIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14868">
        <property type="entry name" value="DUF4487"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="808" mass="91185" checksum="901EE5641D1ED277" modified="2018-12-05" version="1">MSQTNTSLLDEVVQWSQETCRQEIKSVLPKLTISSRILKIITEMFLPHIGLSELEEECFSKILPKAVAMFDNMMKEIMNQVGELSSQNTELCTFLRNILQAMVQLIDALSICVRHVASFEEGLCLDAIRSLPTCILKVLRETFQHCKDSEIVYCGRLSLVADLLQGLFKEAYSLQKSLLEVLDRISLDSSASDEEVSDIVTVIHSLLDICSIISNLDIALHANTWKFLIKQSLKYQSLVEEQLHHGDIVNSLSDNLLASLQSCVETAEQIKLAGLQETVQSPEYKLFQKTAKMCRFFANTLVHYIKEFKYFLTKKSSCFHQLYLQIFSLCAPSLPTALAGELSASALVPMDAFLLQLLPLRPFAEVVLQPDVWLSSEHELPHFLMLVNILGQLLSQPEEVMELWYSGSQFPEDTPRLPLYQAIFISFRQCYTERKVPVRLPGVMLKGQAQVEVTLHQHICVQLCASVAVLPPVFFPVLERCLMDAVLQADTPTALLAVDVWCFTARLVKACNSECYQLLHLGLLLRRMVFLMTPSHQVRLELVAEFSPSKVENLPVWHHVLVRALSQDVRLRVETEIIALMQRTLMEWEDGGYKLGQVDKLQTHLVLQDTLKLLLSATTVIVKNIKPETISQGLLCLEAVVSQKCPDYLLLAALGFLSSLGKIFFPEDCQGQILPRLSGVFGALLADKSWLLHQHALEAFSYFAGNTNHEEVISQSLCVDETKTKVINYLSKVVVDIPSPKRARQETNAEEEYDRHIQTAQNSLKALHALVESKANTTAPPPTWLKAKLQQLMTLIREITSEQVKHLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M111</accession>
    <name>A0A3B5M111_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the phosphatase 2A regulatory subunit B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M111"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013704"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013872.1">
        <property type="protein sequence ID" value="ENSXCOP00000013704.1"/>
        <property type="gene ID" value="ENSXCOG00000010367.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182864"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001892">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000009">
        <property type="entry name" value="PP2A_PR55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018067">
        <property type="entry name" value="PP2A_PR55_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11871">
        <property type="entry name" value="PROTEIN PHOSPHATASE PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037309">
        <property type="entry name" value="PP2A_PR55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00600">
        <property type="entry name" value="PP2APR55"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01024">
        <property type="entry name" value="PR55_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01025">
        <property type="entry name" value="PR55_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU331113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="432" mass="50593" checksum="E571FBF0EF574EC5" modified="2018-12-05" version="1">MEEESDTRKINNSFLRDHNYATEADIISTVEFNSTGELLATGDKGGRVVVFQREQESKTQPHRRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIKWLPQQNAAYFLLSTNDKTVKLWKISERDKRPEGYNLKDEDGRFRDPSTITTLRVPVLQPMDLMVEATARRVFSNAHTYHINSISVNSDLQTFISTDDLRVNLWNLEITDRQGLTHTHTHTHPHTHRCIYTYTLPLIPKHPFHRHIYAHRNPTGLICDFEEPEDPSTRSFFSEIISSISDVKFSHNGRYLMTRDYLTVKVWDLQMENKPLETYQVHDYLRGKLCSLYENDCIFDKFECVWNGSDSVIMTGSYNNFFRMFDRNTKRDVTLEASRENSKPRAILKPRKVCVGGKRRKDEISVDSLDFSKKILHTTWHPHENIIAVAATNNLYIFQDKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M120</accession>
    <name>A0A3B5M120_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ER lumen protein-retaining receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPI-coated vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 14">Endoplasmic reticulum membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the ERD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M120"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013714"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013882.1">
        <property type="protein sequence ID" value="ENSXCOP00000013714.1"/>
        <property type="gene ID" value="ENSXCOG00000010374.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004010"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030663">
        <property type="term" value="C:COPI-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046923">
        <property type="term" value="F:ER retention sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000133">
        <property type="entry name" value="ER_ret_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10585">
        <property type="entry name" value="ER LUMEN PROTEIN RETAINING RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00810">
        <property type="entry name" value="ER_lumen_recept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00660">
        <property type="entry name" value="ERLUMENR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00951">
        <property type="entry name" value="ER_LUMEN_RECEPTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00952">
        <property type="entry name" value="ER_LUMEN_RECEPTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="216" mass="24766" checksum="B23B250E4E34E249" modified="2018-12-05" version="1">MNIFRLTGDLSHLAAIIILLLKIWKSRSCAGISGKSQILFALVFTTRYLDLLTSFISLYNTFMKVVYIGCSYATVYLIYMKFRATYDGNHDSFRVEFLIVPVGGLSVLINHDFSPLEILWTFSIYLESVAILPQLFMISKTGEAETITTHYLFCLGLYRALYLFNWIWRYYFEGFFDMIAIVAGVVQTVLYCDFFYLYVTKGGSCSQFLPGDLVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2W6</accession>
    <name>A0A3B5M2W6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M2W6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017991.1">
        <property type="protein sequence ID" value="ENSXCOP00000017767.1"/>
        <property type="gene ID" value="ENSXCOG00000013385.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156481"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004683">
        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000001">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type II subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000001">
        <property type="entry name" value="calcium/calmodulin-dependent protein kinase type II subunit gamma isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013543">
        <property type="entry name" value="Ca/CaM-dep_prot_kinase-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08332">
        <property type="entry name" value="CaMKII_AD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00056581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="487" mass="55155" checksum="B97CB0A99D2F1E10" modified="2018-12-05" version="1">FEKTNKPKKKTLLSLLAERGRTLYKCVDECLRTLQKYKLEREARICRMLKHPNIVRLHESIAEEGFHYLVFDLVTGGELFEDIVAREYYSEADASEREELAASLCVRSSQMIRLRVLQPENLLLASKMKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPYGKPVDIWACGVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITAEQALKHPWVCQRSTVASMMHRQETVECLRKFNARRKLKGAILTTMLVSRNFSGDSCALLCLYLGLFCMSVFSWNMLASSIPSLHGKVSPASPQEAQTTVVHNPADGTKVSPRKQEIIKMTEQLIEAINNGDFDAYTRICDPGLTSFEPEALGNLVEGMDFHKFYFENLLSKNSKPVHTTILNPHVHLIGEDAACIAYIRLTQYMDSQGRPRSCQSEETRVWHRRDAKWLNVHFHCSGAPAAPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4C6</accession>
    <name>A0A3B5M4C6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4C6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014824"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015010.1">
        <property type="protein sequence ID" value="ENSXCOP00000014824.1"/>
        <property type="gene ID" value="ENSXCOG00000011233.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155312"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042869">
        <property type="entry name" value="ARHGAP11A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15670">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN 11A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15670:SF4">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 11A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="2">
        <location>
            <begin position="44"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="446" mass="49309" checksum="F66C26565C718FD5" modified="2018-12-05" version="1">MNVMEKNVMRLAAVQHLRTVYGIKIKNWNKNKATSCKSTANHLVSLKTFNQHYHICLVFLVDACMKLLAHVETEGLFRKSGSVVRLKELRVSVSHGEECLPTARPCDVAGLVKQFFRELPEPILPTELQDALLKVQQLPSAEERTSATMLLSCVLPDKNICVLRHFFDFLHSVSQRSAENKMDSGNLSVILAPNLLHSGDGTEKMNANTEKCLKLQAAIVHCFIENAQNFGVLPQFLQEKVPAMMGCEPGARSPSQEELEEVNLNSGMKKKNRRSFGDMVCGALNKIKTNRTPTNISQTEGLVFSSTTPVIATPSSKRKLPLEPRNSFGFSNKKRRSVKKTLGFELLPSSLFNGTSTPGSACSASGVLDSSQNTFSSTGRSKRQPSTSTIRKSKRFSHRHIVNRVESGRAGCFSPKVTKKEAPLKSLRLRFSLGRSNKDSVSVAPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M535</accession>
    <name>A0A3B5M535_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M535"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018485"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018717.1">
        <property type="protein sequence ID" value="ENSXCOP00000018485.1"/>
        <property type="gene ID" value="ENSXCOG00000013911.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159376"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000012">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF311">
        <property type="entry name" value="CADHERIN-22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="29"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="186" mass="20256" checksum="A1E34EE17377DE62" modified="2018-12-05" version="1">MAGQMGGLSGSTTVTIVITDVNDNPPRFPQKMYQFSVSEGAAVGTPVGRVIATDADMGENTDMSYLIIDEGDLFKVTTDVETQEAVVSIKKPLNFESKRIHSVIVEAVNKHVDSRFVDLGSFRDQTIVKVTVWDIDEPPIFLPAEGAVMEVQEDAKVGSLIGVVTARDPDVMNKPVRYERDNSPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5T5</accession>
    <name>A0A3B5M5T5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Keratin 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M5T5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015329"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015520.1">
        <property type="protein sequence ID" value="ENSXCOP00000015329.1"/>
        <property type="gene ID" value="ENSXCOG00000011597.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161090"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030280">
        <property type="term" value="F:structural constituent of skin epidermis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031101">
        <property type="term" value="P:fin regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031424">
        <property type="term" value="P:keratinization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000004">
        <property type="entry name" value="Keratin, type II cytoskeletal 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032444">
        <property type="entry name" value="Keratin_2_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003054">
        <property type="entry name" value="Keratin_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616">
        <property type="entry name" value="GATA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616:SF21">
        <property type="entry name" value="KERATIN, TYPE II CYTOSKELETAL 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16208">
        <property type="entry name" value="Keratin_2_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01276">
        <property type="entry name" value="TYPE2KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="8">
        <location>
            <begin position="158"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="543"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="162"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="360"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="562" mass="58684" checksum="48A0F0B76AF4D853" modified="2018-12-05" version="1">MSTRTMSVKTSRTSYGSSGGGGFGGSVRVGGGGGGFSSKSAISIRPSYQMASSSAGGFMQRSSMGGGFSGGSSYGGYGGGAGGGYGGGAGGGYGGGAGFGGGFGAGGGFGAGGGAGGFGGGAGGFIAPPITNVQVNQSLLAPLNLEIDPNIQTVRTQEKEQIKTLNNRFASFIDKVRFLEQQNKMLETKWSLLQEQTTTRSNIDAMFEAYIANLRRQLDGLGNEKMKLEGELKNMQNLVEDFKNKYEDEINKRAGVENEFVLLKKDVDAAYMNKIELEAKVDALQDEINFLRAIYEAELNELQGQIKDTSVIVEMDNSRGLDMDAIVAEVRAQYEEIANNSRAEAEKWYQQKFQEMQTSASQAGDDLRNTKSEIADLNRMISRLQNEIEAVKGQRSNLEAQIAEAEERGELAVKDAKLRIKDLEEALQRAKQDMARQVREYQELMNVKLALDIEIATYKKLLEGEESRIASGGGSATIHVQSSSSSFGGSGGGMGGGMGGGFGMGSGMGGGMGGGYGSGGGYGSGGGYGSGGGYGSGMSIGGGGGSISSKSSSRVSSSRLRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5X5</accession>
    <name>A0A3B5M5X5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">D-2-hydroxyglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="11">1.1.99.39</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the oxidation of D-2-hydroxyglutarate (D-2-HG) to alpha-ketoglutarate. Also catalyzes the oxidation of other D-2-hydroxyacids, such as D-malate (D-MAL) and D-lactate (D-LAC). Exhibits high activities towards D-2-HG and D-MAL but a very weak activity towards D-LAC.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(R)-malate + A = oxaloacetate + AH2</text>
            <dbReference type="Rhea" id="RHEA:67460"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15588"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:67461"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.39" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M5X5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018785"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019021.1">
        <property type="protein sequence ID" value="ENSXCOP00000018785.1"/>
        <property type="gene ID" value="ENSXCOG00000014141.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075086"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051990">
        <property type="term" value="F:(R)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="498" mass="54711" checksum="3EF4CCA36F0EC7E1" modified="2018-12-05" version="1">MKGKYRRTATFSRELHLGPGGSASSPAAAPQRLPFSRVTVEDLTFFRKTLPGRAITDPDLVQSSNEDWLKSVRGSSEVLLRPQTTQEVSQILSYCQSRNLAVNPQGGNTGLVGGSVPVYDEIILSTSLMNNILSFDKISGILTCQAGCVLENLSLYLEERDYIMPLDLGAKGSCQIGGNVATNAGGLRLLRYGSLRGTVLGLEVVLADGQVLDCLSTLRKDNTGYDLKQLFIGSEGTLGVITAVSILCPRKPKSVNVVFLGCETFDQLLKSFQLCKGMLGEILSAFEFLDSECMRLLNTHLKLANPISDCPFYIVIETSGSDSDHDSQKLHNFLEEAMTSLLVTDGTVATEESKIKTLWSMRERVTEALTHDGFTYKYDISLPVERIYQLVTDMRQHLGERARSVVGYGHVGDGNLHLNITSSAKDPALLASIEPFVYEWTARFQGSISAEHGLGLKKRNYIYYSKSRPTVAIMGKMKTLLDPKGILNPYKTLPDDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5Z3</accession>
    <name>A0A3B5M5Z3_9TELE</name>
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            <fullName evidence="8">Dorsal inhibitory axon guidance protein</fullName>
        </recommendedName>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSXCOT00000019098.1">
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        <property type="gene ID" value="ENSXCOG00000014197.1"/>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:axon guidance"/>
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        <property type="term" value="P:Wnt signaling pathway"/>
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        <property type="project" value="InterPro"/>
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    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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            <begin position="48"/>
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    </feature>
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            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018862.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="17823" checksum="A2D3F8D79A51BB4C" modified="2018-12-05" version="1">MVTTATNEHPPTRPPGSAKPQKSGQGQGEVMPTLDMTLFDWTDYEDMKPVDSWPSSRKKDKRRSKNLSSGNLTADVDAIEPCDHHLDCLPGSCCDLRQHECKPHNRGLNNKCYDDCMCEEGFRCYAKFHRKRRVTRRRGRCVVPESVSNDHGGFITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M625</accession>
    <name>A0A3B5M625_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Bromodomain containing 7</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M625"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016856"/>
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        <property type="gene ID" value="ENSXCOG00000012715.1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="BROMODOMAIN CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR22881:SF12">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
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    </dbReference>
    <dbReference type="Pfam" id="PF12024">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
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    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="8">
        <location>
            <begin position="131"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
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        <location>
            <begin position="240"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="261"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="274"/>
            <end position="287"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="583" mass="65657" checksum="3E776854E3CC48C5" modified="2018-12-05" version="1">MGKKHKKHKSEKHAYEEYGERPLKLVLKVSGNEVTTGSSSLDTFYEEQPVDSDKPKDKKKKKKKDKDRSFGSPEEDRGKKKMTKKKKGHDADGEEDREQSRTPIRSELDKLEEKEQTPLQEALNQLIRQLQRKDPNAFFAFPVTDLIAPGYSSIIKRPMDFSTMKDKVKKEFYQSLDELKVDFRIMCENAMIYNKPETIYHKAARKLLHSGMKILSQERLDSLKQSIDFMSGLDRFAKQPVKSEDQGVTSSDQSKEGAMTAGSSERSQTPITPRQDKDSKDEAAKAEKELEEIRKVIEESGGKLSNRVVQCDLEFARRKSDGSTTLAVLNPADPSPGDVGYCPVKLVMMSNRLQNGVNSLQSFREDKRNRVTPVSYLSYGPFTSYAPAYDSSFSNVSKDDSDLIYSFYGEESSMQGSDSLSEFLAKSNEYVYKMGDSLLDAMTNGEHSRCLKETEVDFEGSQHNELMLIIIHKSVRADPPLSVTDALHFQQKLDETTKLLRNLQEVQRERLSAKQPPNMICLLAPTAKELELAEKVTGNLTELTGQVAPCDVSSIYGIRKAMGITVPPELPEPLIDLTTGKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M734</accession>
    <name>A0A3B5M734_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arginine vasopressin-induced protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in MAP kinase activation, epithelial sodium channel (ENaC) down-regulation and cell cycling.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M734"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019280"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019518.1">
        <property type="protein sequence ID" value="ENSXCOP00000019280.1"/>
        <property type="gene ID" value="ENSXCOG00000014501.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234958"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039579">
        <property type="entry name" value="AVPI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020282">
        <property type="entry name" value="Avpi1/C8orf4_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14350">
        <property type="entry name" value="ARGININE VASOPRESSIN-INDUCED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15063">
        <property type="entry name" value="TC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginine vasopressin-induced protein 1/transcriptional and immune response regulator" evidence="5">
        <location>
            <begin position="17"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF15063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="144" mass="16384" checksum="069E4241E5AE25C0" modified="2018-12-05" version="1">MAEALEVSSTEDCLPAEWKPFSRRSRKSGCSNIFSGVNLHQLHKLFRTAGDRNAEHRAKLVWQDVEGNPEGAEEGKQSEEEQEPLAVYSVEDNADQSESLETFKLPADEDITGNQNPFKPSSWRLGIRMESARDSERYLHRILH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M801</accession>
    <name>A0A3B5M801_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RFX-type winged-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000020408"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020656.1">
        <property type="protein sequence ID" value="ENSXCOP00000020408.1"/>
        <property type="gene ID" value="ENSXCOG00000015286.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016138"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052406">
        <property type="entry name" value="Chromatin_Remodeling_Comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003150">
        <property type="entry name" value="DNA-bd_RFX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22970">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22970:SF14">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02257">
        <property type="entry name" value="RFX_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51526">
        <property type="entry name" value="RFX_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RFX-type winged-helix" evidence="6">
        <location>
            <begin position="405"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="495"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="600"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="713"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="780"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1062"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1143"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1215"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1389"/>
            <end position="1414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="495"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="520"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="570"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="733"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="781"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="799"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="812"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1068"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1215"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1237"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1390"/>
            <end position="1403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1495" mass="158255" checksum="36BEAE179EA29C82" modified="2018-12-05" version="1">DFSTSGNTRPSIHLDRLPSDYLRQSYGLSTDFTQPCDYNKLVLSLLSGLPNEVDFAVNVCTLLSNESKHAMQLDKDPKLVTLLLAHTGVFDDSLGNFSTVFGTDWKEKTSRDFVRFWKEVVEDTEVRELIWDKSSSAQDGTFREDHWQSLFHPPRNPGTSDMEAQRVMQVAVILRNLSFEEANVKLLAANRTCLRFLLLCAHCNLISLRQLGLDTLGNVAAELQLDPVDFRTTHLIFHTITKCLMSRDRFLKMRAMEILGNLSKADDNGVLICEYVDQESYREVIMLLTLPDLMLLMASLEVLYLLAQLGEIPCSKIASVDHSIDLLVRLVSVDLHTFGPDALTAVRLIEHQANAEQAAEVRPQLVEQVPAAVQGATAPGKVTRVPVQSNVPPPGIVELDGEKFTLQWLNAHFETNPEGSVSRSEMYSEYLATCSKMGRGNILNSTGFLKCLRTVFPNHTMRRQDEAKASNPLQILLVGIRRRAIPLPIQLYYQQTQQQHQNPAAAPPPPVARHEAPGDPQAPPPGTSGLPPGHPNPGPQFVRSLGVSNSAPPMASPAQESSRVNHPAVPRHPGPPQASPQLPPTLVRTAPGVQITTSAAAAQNHSPQNPAPPPVPQQQHSPMLPTGAPVTLFQPVPQGHILTARVQGVCSQVTQQPPLPQTLPVSGPQGGAPQVDAQCTATVSGPPSIQVGGSQAVGIMGVANSQGSRVTFQNIAPKPAPNQSGGPTATLAPPNQQPQSQQSVVIVSPNPQQNAAYTPAIHQIVLANPSSIPGAQTVQIAGQPGAASSPCSPAASHSNTQVQSNQAVSHALSTIRQQQQQPLPPLQIISQTPPTQPTSTESSLIKQLLLPKRGPSTPGGKLILPAPQVPLPNNTIAPNPQVIYQNPSPQPVSVQLVPGQLPAAGAPPLQAVQLLPGQLISTSTPGGAAIIQAPTAAGQVTFTVVPNTGFTTSAAAVAAVSQGAVAPGVPPPPFSTGTVPHHVSTGAPLPPPLPAPLRGDKIICQKEEEAKDATGLHIHERKIEVMENSSLAEGDPSNNKTRNGDVAAGAKLLNGGKCVEPNLPPYHSGNSQGVLNGPVTESLSSNGKQALSPGPNAPLEGNADPKKTLVNGVCDFERGDSGGNFNKNIPNHIASKQYLGNGEVGASEKTSDQSLPSPPPPQQDTAKAQQAERLANGPQAAAHRPPSELTNGPLGPALGHGTPVLRQQLLPNSSLPCAVTSQNPAASPANGVASEPRGLKRPAENDERGPTAVATSSGIPSKVGVRIITISDPNNAGSSATMVAVPAGTDPSTVAKVAIENATQQRNCSPTQAAGATVSHSGVSSMWFETPSQVFYHAATQHGGKEVYGGHCLWEGCEPFPRQRLSFITHLQDKHCSREALLAGLKLEEQQAQSPNQTSSQTPPAAGSTPAQRAPKAIVNHPSAALMALRRGSRNLVFRDFTVSTTRLTHVSVLSYSGFIRVLEILVKALNSIGGLESVSYSNCSFVIKRSLKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M919</accession>
    <name>A0A3B5M919_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M919"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020037"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020284.1">
        <property type="protein sequence ID" value="ENSXCOP00000020037.1"/>
        <property type="gene ID" value="ENSXCOG00000014898.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159633"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:MAP kinase kinase kinase activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="C1_A_C-Raf"/>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
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    <keyword evidence="8" id="KW-0418">Kinase</keyword>
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    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="23"/>
            <end position="95"/>
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            <begin position="102"/>
            <end position="148"/>
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    </feature>
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        <location>
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            <end position="570"/>
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        <location>
            <begin position="1"/>
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            <begin position="247"/>
            <end position="288"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="13"/>
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        <location>
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            <end position="283"/>
        </location>
    </feature>
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        <location>
            <position position="330"/>
        </location>
        <ligand>
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        </ligand>
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        </source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="586" mass="66528" checksum="9B0AD4E3D1C47263" modified="2018-12-05" version="1">MSSTSSYSSSGETSPEDVPRGGGTIRVYLPNKQRTVVNVRQGQTVYESLNKALKVRGLSQDCCAVFRLLEGRKRLTDWDTDITPLVGEELLVEVLDDIPLTMHNFVRKTFFKLAYCDFCHKFLFNGFRCQTCGYKFHQHCSSKVPTVCVDMDTVSKRCDSNSCTDYPQILLPENSPSQSNLALTPESGLLSPTFNFPTTGGDGQSLQRHRSTSTPNVHMVSTVDITNVVIIEVSFVWTVLLYIKPSPKPPTSPPSSLGSPGRRLQKSPLEHKERKSSSSDDKKKVHRGGYRDSSYYWEVPYREVTLQKRIGTGSFGTVFKGKWHGDVAIKILKVKEPTPEQLQAFKNEMQVLRKTRHVNILLFMGFMTKPNFAIITQWCEGSSLYRHLHVTETKFDTMRRIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGWTVKIGDFGLATVKYRWSGSQQVEQPSGSILWMAPEVIRMQDSNPYTFQSDVYGYGVVLFELMSGTLPYSNINNRDQILFMVGRGYLSPDLGKLCSTSPKSMKRLIIDCLKFKREERPLFPQHIIPQILQSDHFTVSNENITVRLLLKGHKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9G5</accession>
    <name>A0A3B5M9G5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Delta-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Putative Notch ligand involved in the mediation of Notch signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9G5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016404"/>
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        <property type="gene ID" value="ENSXCOG00000012387.1"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:diencephalon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:midbrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="Delta-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000347">
        <property type="entry name" value="delta-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000004">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="7"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="EGF"/>
    </dbReference>
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        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011651">
        <property type="entry name" value="Notch_ligand_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12916:SF9">
        <property type="entry name" value="NEUROGENIC LOCUS NOTCH HOMOLOG PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21700">
        <property type="entry name" value="EGF_DL_JAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07657">
        <property type="entry name" value="MNNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
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        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
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    <sequence length="634" mass="69827" checksum="8C673CCCC03FE576" modified="2018-12-05" version="1">MYFTVYLTMITVALSQVWSSGVFELKIHSFHTAQRICRRHRDCHIFFRICLKHPEDVISAEPPCTFGTGHTNVIRADHTSISSSAPIRVPFHFKWPGTFSLIIEAWNAESPTEYTDNQNNLVSRLATRRRLAIGEDWSQDVHFGEQSELRYSYHVYCDEYYFGDGCADYCRPRDDTLGHYTCDEEGNRICLEGWKGNYCSEPICSADCSEKHGYCEAPGGCTCRMGWQGPSCNECVRYPGCLHGTCSQPWQCNCQEGWGGLFCDQDLNYCTNHKPCANGATCTNTGQGSYTCTCRPGFGGTNCELETNECDSNPCKNGGSCNDLENDYSCTCPQGFYGKNCEIIAMTCADGPCFNGGTCVETMTGGYTCRCPPSYTGSNCEKKLDRCSNRPCLNGGDCLDLGQSILCRCQPGFTGANCQVNIDDCTSNPCQNAGTCQDGVNDYTCSCTLGYTGKNCSIRSDACGARPCQNGGTCFTHFTGPVCQCPKGFMGPSCEFTLQPSFKPALRQASQPSSTTTVTISCLLAVLVLVLVAGIFFLRRRRRRMEGRKQLSDIAVYNDLEAVNNLGGSERDAFLSPNSLFKISNSTARLSLTLCPDGRPGYRHNPVESSLARAERQDFMWRDDAMLGSAPGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9J1</accession>
    <name>A0A3B5M9J1_9TELE</name>
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            <fullName evidence="20">Voltage-dependent P/Q-type calcium channel subunit alpha-1A</fullName>
        </recommendedName>
        <alternativeName>
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        </alternativeName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Membrane</location>
            <topology evidence="24">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1A subfamily.</text>
    </comment>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="6 24" id="KW-0107">Calcium channel</keyword>
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    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
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    <keyword evidence="8 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
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    <keyword evidence="12 27" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="11 24" id="KW-0851">Voltage-gated channel</keyword>
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            <end position="121"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="980"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="26">
        <location>
            <begin position="1437"/>
            <end position="1451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="1490"/>
            <end position="1528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="1550"/>
            <end position="1562"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="609"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="23">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00037936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00039688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00041622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU003808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="SMART" id="SM01062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1562" mass="177127" checksum="D2F119C5E7CE4BEB" modified="2018-12-05" version="1">MARFGDELPNRYGGGGGGGGGGGGGAGGQGGPGRGGSRQGGPPGAQRMYKQSMAQRARTMALYNPIPVRQSCLTVNRSLFLFSEDNVVRKYAKKITECCTPFEWMILTTIIANCIVLALEQHLPDGDKTPLSERLEETEPYFIAIFCFESGIKILALGFALHKGSYLRNGWNVMDFVVVLTGHLEEILGMSSLKVVLLPSLPLSLLLCSHLKPHDEKVEDRPCGPKLPSRLCPNDSSCESGWIGPNYGITQFDNILFAILTVFQCITMEGWTDMLYYSNDVEGSMWNWMYYIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLRRQQQIERELNGYLEWICKAGQFMLMDTFHSNTHSSRRRPTIKTRNNKTELLNQEEGEDNIGDAVGFARSSIKSGKDGANYSTKERRMRFFIRKMVKTQAFYWTVLCLVGLNTICVAVVHYNQPELLSDFLFYAEFIFLGLFMSEMLIKMYGLGIQPYFHSSFNCFDCVIVGSIFEVVWATIKPGTSFGISVLRALRLLRIFKVTKYWAPLRNLVVSLLNSMKSIVSLLFLLFLFIVVFALLGMQLFGGLFNFEEGTPPTNFDTFAAAIMTVFQILTGEDWNMVMYDGIESQGGVKDKGMVFSIFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEEQEEAANQKTALQKAKEVAEVSPLSAANLSIAAKEQQKNHTKPNKSVWEQRTSEIRRQNLMTSREALYNELEQDDWKVSFPRKARGDVKTHLDRPLVVNPQDNCNNNTNKTPPGELPLDQQYRRQDVDHCRRAAHHCHYHRHHHQKSTNPDGVDATQLMETNMGHGFSCTSLGNVEGNVERHGDEEKHSHQSHRGHRHRNRECREARSVSPHHGIGADGNGEARRCRQHRRAAREADEGRRHQCRATEGDGEKSEEEGGQKARRHRHCNQERCRGHRVRKVRPPSVSLSLIYPLCLPSSLCSVNKNANTDQKKSEEKKKEEDEGEDEDNAKRMPPYSSCFIMSTTNPFRKCCHYILTLKYFEFSILSVIAMSSIALAAEDPVKPDSPRNNVLRYFDYVFTGVFTFEMLIKMVVLGLFLHQGSYFRDLWNILDFIVVSGALVAFAFTKAQNLPKKIIVFLCVIHMLSYCLCHFIAKITTLDSRKPRCQGHYYFLFYCVFEEETAINEHNNFRTFIMALMLLFRSATGEAWHDIMLACLGEKKCDPESGSTGKECGSTFAYTYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKHQMDAELRKEMMAIWPNLSQKTLDLLVTPHKATDLTVGKIYAAMMIMEYYRQSKTKKLQALREEQNRTPLMFQRMEPPSEGGGTEGQAGPGQNGLPGVQPDNINSGLTESQSWMTAKAQEMFQKTGNWSPDRPYPDDVHENRHNPQTVEMREMGRDGYSDTEPCHPVDGHGRTLSMPRLSADNQRRRHRPRGNNLSVCTSLSVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9Q4</accession>
    <name>A0A3B5M9Q4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Si:ch211-145o7.3</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9Q4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020993"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021251.1">
        <property type="protein sequence ID" value="ENSXCOP00000020993.1"/>
        <property type="gene ID" value="ENSXCOG00000015706.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166209"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047119">
        <property type="entry name" value="FOXN2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13962:SF26">
        <property type="entry name" value="FORKHEAD BOX PROTEIN N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13962">
        <property type="entry name" value="FORKHEAD BOX PROTEIN N3-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="120"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="120"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="18"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="43"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="314" mass="34316" checksum="CE380868B3994F3A" modified="2018-12-05" version="1">MKNGSHTLPPFSSSLAALGGSLTLTSSPPSLPLSPVSFPPSPTSLSPAAAESARSSSPVSDLAASQCLGQGDTSDQDDLTCLSWLHQRGDLLPLQPLSKVTTLPQRQPGQLPPPVAASSKPPYSFSSLIFMAIEDSPQKRLPVKDIYGWVVSNFPYYKTATGGWRNSVRHNLSLSKSFRRIQRDKSQLQSVGKGSLWCVCPEYRPALLEGLRKTHSYHGTSSCLINKPALPCPLTPDHEELVTMEPHEYQLHEVSEDTEKDPLSDSGYIELHYYESDQCQYLVLPSESELDLETVEILQLDVEAQEAAGSLLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MA07</accession>
    <name>A0A3B5MA07_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MA07"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021083"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021342.1">
        <property type="protein sequence ID" value="ENSXCOP00000021083.1"/>
        <property type="gene ID" value="ENSXCOG00000015771.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271896"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070915">
        <property type="term" value="F:lysophosphatidic acid receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15342">
        <property type="entry name" value="7tmA_LPAR2_Edg4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000025">
        <property type="entry name" value="Lysophosphatidic acid receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004065">
        <property type="entry name" value="LPA_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002277">
        <property type="entry name" value="LPA_rcpt_EDG2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22750">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01148">
        <property type="entry name" value="EDG2RECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01527">
        <property type="entry name" value="LPARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="195"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="240"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="55"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="354" mass="41491" checksum="1AF4B4FBE475F86C" modified="2018-12-05" version="1">MVRTEFCLGVTVCYYNETVEFFYNNSNKTIAPFWRKRDYAVVSLGLLVCFIVIFSNLLVIFAIFKNRRFHFPIYYLLGNLAVSDLFSGLAYLHLMFHTGPWTIRLSQYQWFVRQGLIDTSLTASVLNLLAIAMERHQTIFNMQLHSEMSTRRVFIIIFFIWMVSIIMGLFPTMGWNCLCDLERCSTMAPLYSRSFLVFWALTNLITFSIMVAVYTRIFFYVRHKSRQMSEHTTPARHRETVLGLMKTVSMILGLFVLCWTPGLVLLMLDGLYCETCLVLNYEKYFLVLAECNSFVNPIIYCFRDKDMRKTFKELLCCLCRRGKGPSDNDFTTLEVMSETNKDDNTNHKQKNYSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MB46</accession>
    <name>A0A3B5MB46_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial glycine transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Solute carrier family 25 member 38</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SLC25A38</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">May play a role as pro-apoptotic protein that induces caspase-dependent apoptosis.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Mitochondrial glycine transporter that imports glycine into the mitochondrial matrix. Plays an important role in providing glycine for the first enzymatic step in heme biosynthesis, the condensation of glycine with succinyl-CoA to produce 5-aminolevulinate (ALA) in the miochondrial matrix. Required during erythropoiesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>glycine(in) = glycine(out)</text>
            <dbReference type="Rhea" id="RHEA:70715"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the mitochondrial carrier (TC 2.A.29) family. SLC25A38 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MB46"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020812"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021065.1">
        <property type="protein sequence ID" value="ENSXCOP00000020812.1"/>
        <property type="gene ID" value="ENSXCOG00000015597.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075117"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015187">
        <property type="term" value="F:glycine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030218">
        <property type="term" value="P:erythrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904983">
        <property type="term" value="P:glycine import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042541">
        <property type="term" value="P:hemoglobin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000036">
        <property type="entry name" value="Mitochondrial glycine transporter B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03064">
        <property type="entry name" value="SLC25A38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030847">
        <property type="entry name" value="Hem25/SLC25A38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46181">
        <property type="entry name" value="MITOCHONDRIAL GLYCINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46181:SF1">
        <property type="entry name" value="MITOCHONDRIAL GLYCINE TRANSPORTER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 11 13" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="15"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="116"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="202"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="289" mass="31053" checksum="EDE18CF620C370CE" modified="2018-12-05" version="1">MNGRAPTHTHTHTAHPAVKAFVCGSFSGTCSTLLFQPLDLVKTRLQTSQSGMQPGSSRVGMTSVLLGVVRTERLLGLWKGSSPSFVRTIPGVGIYFSSYHSLKQHFFQDSRPGAAQAVMLGGGARTVAGVVMLPITVIKTRFECGRYSYVSVSGALRSVCRSEGPAALFSGLMATLLRDVPFSGIYVMFYSQTKALLPLSPSAPVANFSCGVLAGVLASLVTQPADVVKTHVQVNPQLRTTKAVRHIYTAHGLQGFFRGAVPRCLRRTMMAAMAWTVYEQMMAHVGLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDP3</accession>
    <name>A0A3B5MDP3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDP3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018043.1">
        <property type="protein sequence ID" value="ENSXCOP00000017819.1"/>
        <property type="gene ID" value="ENSXCOG00000013426.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234575"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03590">
        <property type="entry name" value="CLECT_DC-SIGN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033989">
        <property type="entry name" value="CD209-like_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017339502" description="C-type lectin domain-containing protein" evidence="4">
        <location>
            <begin position="20"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="5">
        <location>
            <begin position="98"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="233" mass="26070" checksum="21770A6B970D3C15" modified="2018-12-05" version="1">MLASVSLLVLIRFCSKCSSGENPTRTSKLPAGGVVLLVLVGLLAAALIVIYRLCEFILINFSWILLYQMSAEKLQSSLCSDLFEGETFLKCEAGWEKHGGNCYYFSTSKSSWINSRRSCVDLGSDLVKIDSREEQLFLEIRLRGLMMKDTDKFWIGLTDSVEEGRWFWVDGSPLDESQKEPDDWKGENPAGEDCVRVGERGGSHDLTCWFDKACNVSLKSICEKPAAPGRRCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5ME08</accession>
    <name>A0A3B5ME08_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transmembrane protein 236</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5ME08"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021877"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022144.1">
        <property type="protein sequence ID" value="ENSXCOP00000021877.1"/>
        <property type="gene ID" value="ENSXCOG00000016346.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015525"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020394">
        <property type="entry name" value="Uncharacterised_FAM23-like_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31453">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31453:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="349" mass="39539" checksum="10DD5875235D0922" modified="2018-12-05" version="1">MVPSGRTVKLIVYEVLQFAALIVPVFVVMERFARIIRDVKGQNVTAYWLVVAASIAYVTSATLLVWVPLKYVILKKRRFILEITQWRPTALAYLLLSTLPCFAILIASSKVIPSRLITAQQKLDHFTELPVSLVLFSLICVDIIERIRPHKLMGMKQKGPVLTQLTHVTSVSGQLPADEGQNSVTPGQAEARNGSTTNKRQDVTESPHSTRPNASYLYSSTPRLMPYSGPLSFLWRKDGRAEVFVECFLFWLDTVEMVRVSGEPVIFYSAWVFPVYILAFLSCLRMIIAPHSFLLPFAGAMLQDLPFFIIRVALIIVFGFVTPLLYPLKNVLVSLTFIYFTWMTKLRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEF9</accession>
    <name>A0A3B5MEF9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase</fullName>
            <ecNumber evidence="9">3.6.1.62</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + H2O = N(7)-methyl-GDP + a 5'-end phospho-ribonucleoside in mRNA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:67484"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:63714"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="EC" id="3.6.1.62"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67485"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DCP1 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.62" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEF9"/>
    <dbReference type="SMR" id="A0A3B5MEF9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018044"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018271.1">
        <property type="protein sequence ID" value="ENSXCOP00000018044.1"/>
        <property type="gene ID" value="ENSXCOG00000013593.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158409"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140933">
        <property type="term" value="F:5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09804">
        <property type="entry name" value="Dcp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000097">
        <property type="entry name" value="Putative mRNA-decapping enzyme 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010334">
        <property type="entry name" value="Dcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290:SF5">
        <property type="entry name" value="MRNA-DECAPPING ENZYME 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290">
        <property type="entry name" value="TRANSCRIPTION FACTOR SMIF DECAPPING ENZYME DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06058">
        <property type="entry name" value="DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="125" mass="14214" checksum="63E7A6BFFD123A75" modified="2018-12-05" version="1">MAASNSCLAAKGLDISLSALQRQDPYINNIVDVASQVALYTYNNRANEWEKTEVEGTLFIYTRLASPRHGFTIMNRLNMENLTEPITKDLDFQLQDPFLLYRNARRNGLNGPVKVQTSVNLRFCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGM5</accession>
    <name>A0A3B5MGM5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tripartite motif containing 35-12</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGM5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023749.1">
        <property type="protein sequence ID" value="ENSXCOP00000023468.1"/>
        <property type="gene ID" value="ENSXCOG00000017523.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00970000193381"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="B302_SPRY"/>
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        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="11"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="79"/>
            <end position="120"/>
        </location>
    </feature>
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        <location>
            <begin position="269"/>
            <end position="456"/>
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    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="121"/>
            <end position="148"/>
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    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="175"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50119"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50188"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023468.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="458" mass="51431" checksum="56898167D2BBB6E0" modified="2018-12-05" version="1">MASGSEKNLSCPICHEIFTDPVVLSCSHSFCQACVQSWWKEKLINECPVCKERPLSNPPISSALRNLCLAEKTRLSAGAEPGLCSQHSEKLKLFCLDHQQSVCVVCRDSKAHSNHRFRPIDEAAEERREEVRESLTALREQLKLFEEAKGSRDQNAKHIKSQNRNTEKQIRDQFRKFHQLLQEEEEARIAALREEERQKSKALREESEALSRIISDFSTTIRETEKQLKADSVSFLRSYKAALTTAEQRRLQDDPAPSAGALLDVAKHLGNLAFNIWIQMKAAVCFSAVVLDPNTAHPELTPDDDLSAATHGERLKLPDNPERFGWCRAVLGSDGFTSGSHSWEVEVGEGGGWEVGVAAESADRKGSVGSGSWRIGLDGAFLAFFPPGRSSVLSVKPPRRIRVHLDWERGKLSFSDADTGGHIHTFSHCFTEKLFPFFSFKNRLFIYIWTVPTKSNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKB5</accession>
    <name>A0A3B5MKB5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">[histone H3]-trimethyl-L-lysine(4) demethylase</fullName>
            <ecNumber evidence="4">1.14.11.67</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 2-oxoglutarate + 3 O2 = L-lysyl(4)-[histone H3] + 3 formaldehyde + 3 succinate + 3 CO2</text>
            <dbReference type="Rhea" id="RHEA:60208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.67"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JARID1 histone demethylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.67" evidence="4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024819"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025120.1">
        <property type="protein sequence ID" value="ENSXCOP00000024819.1"/>
        <property type="gene ID" value="ENSXCOG00000018532.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157076"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034647">
        <property type="term" value="F:histone H3K4me/H3K4me2/H3K4me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16874">
        <property type="entry name" value="ARID_KDM5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15603">
        <property type="entry name" value="PHD1_KDM5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15687">
        <property type="entry name" value="PHD3_KDM5B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Lysine (K)-specific demethylase 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000035">
        <property type="entry name" value="PHD transcription factor Rum1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.60:FF:000001">
        <property type="entry name" value="Putative lysine-specific demethylase 5b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000001">
        <property type="entry name" value="Putative lysine-specific demethylase 5b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048615">
        <property type="entry name" value="KDM5_C-hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047981">
        <property type="entry name" value="KDM5B_ARID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047978">
        <property type="entry name" value="KDM5B_PHD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047979">
        <property type="entry name" value="KDM5B_PHD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013637">
        <property type="entry name" value="Lys_sp_deMease-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004198">
        <property type="entry name" value="Znf_C5HC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF136">
        <property type="entry name" value="[HISTONE H3]-TRIMETHYL-L-LYSINE(4) DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21323">
        <property type="entry name" value="KDM5_C-hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08429">
        <property type="entry name" value="PLU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02928">
        <property type="entry name" value="zf-C5HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="22">
        <location>
            <begin position="27"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="ARID" evidence="21">
        <location>
            <begin position="92"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="20">
        <location>
            <begin position="291"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="23">
        <location>
            <begin position="435"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="20">
        <location>
            <begin position="1158"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="20">
        <location>
            <begin position="1382"/>
            <end position="1436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1313"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1313"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="1343"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1363"/>
            <end position="1376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012902"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1442" mass="164215" checksum="EDFBDA62105215CC" modified="2018-12-05" version="1">DDGSTCLHPLYWFTASSLNEFIPPPECPVFEPSWEEFADPFAYINKIRPIAEKTGICKIRPPPEWQPPFACDVDRLKFTPRIQRLNELEAQTRVKLNFLDQIAKFWELQGCTLKIPHVERKILDLYQLNKLVNEEGGFDAVCRERRWTKISVKLGFAPGKAIGSHLRAHYERILYPYNLFQIGSNLYCYIIFSHQIVLLEYTPHDLPQRQSVQPQETCSIARRAKRMRSERLSFKKEPGEICENRPNLRRRMGTYVGTEIKQEPTDHMDPKEHEETDLNKINKPPQNKVDQYMCLICGSGSAEDRLLLCDGCDDSYHIFCLIPPLHDVPKGDWRCPKCLAQECGKPPVAFGFEQASRSYTLQAFGDMADSFKSDYFNMPVHMVPTELVEKEFWRLVSTIEEDVTVEYGADIASKEFGSGFPVRNSHFEVSPEDEHYLTSGWNLNNMPVLDASVLTHITADICGMKVPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGAPGYAAEHLESVMKKLAPELFESQPDLLHQLVTIMNPNTLMNNGIPIYRTNQCAGEFVITFPRAYHSGFNQGFNFAEAVNFCTMDWMPIGRNCVDHYRQLSRYCVFSHDEMVCNMAFKADTMDVELASALLEDMTAMIQEERELREKIDKMGVVQSRQVDYELLPDEGRQCCKCRTTCYLSGIVCSCSPDKMACLYHAQYLCSCPNSNLTLHFKFTLDELYPLMESVKLRSQSYKEWLSAVEDIVENKGAKKKGELHSLVEQAETKAFPKISLLDQLRTVTSEADKVAVMAQQLLNGKRQTRYRSGGGKSQNQNELTVEELRSFIQQLDNLPCNIRQAPLLKDLLTRVDDFQQRSNRLLSDEAPSPQELQELLDVSLGLDVELPQLPLLRERLEQARWLEAVQQASSRPDSLCLDTMRRLIDQGVGLAPHSSVERAMARLQELLTVSEQWEERVLGLMDTRPYPSIEMLDAALQEVENIPAYLPNCLQLKDVVTKAKKWLNEAEMLQMGGRIPVLDSLSELVLRADGIPVRLDLLGRLEALISDVQSWKESAAKTFLLKNSPLSLLEVLCPRCDVGSGHQKCRSKKVKEALQLNKKASLKLELVCDVERALSESKDSPSAMSALADVRQREMELLLALRASNESKLLPAENSSALTVCVCQKAPSGAMVQCELCRDIFHRDCVTTAAELEYGQAWLCPLCQRSRKPPLDKVLPLLASLQRIRVRLPEGDALRFLIERTVRWQHKVQQACTDGLLERVATTFLFNSNGNAFVAALGPELEELMVEGFLLQVTLPETEQLYRYLLYKLAPPSSHNSPSRNGAEQSDEGSPRHNKVQIDLWAKKSRKKKPKKSKERSDESKRTSSPSHTSSDPALSDSEEDFSLCAAPWCREPEGDEVNWVQCDGNCNQWFHQVCVGLSAERAEKEDYICISCTQPDYDRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5ML13</accession>
    <name>A0A3B5ML13_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5ML13"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025079"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025384.1">
        <property type="protein sequence ID" value="ENSXCOP00000025079.1"/>
        <property type="gene ID" value="ENSXCOG00000018726.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153424"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000110">
        <property type="entry name" value="Death-associated kinase 3, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000250">
        <property type="entry name" value="Death-associated protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342:SF18">
        <property type="entry name" value="DEATH-ASSOCIATED PROTEIN KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24342">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="309"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="438" mass="50813" checksum="54DE2927AF78EC7D" modified="2018-12-05" version="1">LSLSSGQFAIVRKCKEKRTGVEYAAKFIKKRRLSSSRRGVSREEIEREVNILREIQHSNIITLNDIFENKTDVILILELVSGGELFDFLAEKESLTEEEATQFLKQILDGVHYLHSKRIAHFDLKPENIMLLDKNVPNPRIKLIDFGIAHQIKAGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGETKQETLTNISAVNYDFDEEYFSNTSELAKDFIRRLLVKDPKKRMTIDDSLEHPWIKVIKRRNVRQEDRNHKTERRRLKTTRLKEYTIKSHSSMPPNNTYVNFERFSQVLEEIAAAEEGLKELERNQRSCQEDVAALLSIYEEKEGWYKEENQSISSDLSHIRQELQRSQTQRRKCQEDARLTMQSANNLKRKFGRLENRYDALSEQVALEVRWVEELLRSMSAEKDALGSGSVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLZ5</accession>
    <name>A0A3B5MLZ5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Leucine rich repeat containing 58b</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027880104.1">
        <property type="nucleotide sequence ID" value="XM_028024303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLZ5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025429"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025736.1">
        <property type="protein sequence ID" value="ENSXCOP00000025429.1"/>
        <property type="gene ID" value="ENSXCOG00000018997.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114148823"/>
    <dbReference type="CTD" id="564300"/>
    <dbReference type="GeneTree" id="ENSGT00940000156556"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050715">
        <property type="entry name" value="LRR-SigEffector_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45752">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45752:SF13">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="353" mass="39955" checksum="80E8D45C4E17E617" modified="2018-12-05" version="1">MESQEGAAAATRDCVLDFSRLSLNTFNVNNVSEDRQRDTRQLFLNYNRLYALPSSVSLFYNLELLDISNNGLTTICEGITRLAKLKTLLAKNNRLDEFSLPKEFGSLQLEVLNFSGNRFQEIPLQCTKLLRLQSLSIGGNRLKSIPAEIEYLTSLEMLYLGGNLISDIPPELAHLPYLTYLVLCDNRIQSVPPQLARLHSLRSLSLHNNLLTYLPREILSLVHLQELSLRGNPLVVRFVKEMIYDPPSLLELAGRTIKSRNIPYFPYDMPSNLVLYLDLASKCPNPKCAGVYFDSCVRQIKFVDFCGKYRLPLMHYLCSPECTSPCSSNPQSDAESEEENSVPADRLQRVLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MM48</accession>
    <name>A0A3B5MM48_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">F-box and WD repeat domain containing 9</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MM48"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024636"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024934.1">
        <property type="protein sequence ID" value="ENSXCOP00000024636.1"/>
        <property type="gene ID" value="ENSXCOG00000018409.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006806"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
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        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044715">
        <property type="entry name" value="WDR86-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44489">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44489:SF11">
        <property type="entry name" value="WD REPEAT DOMAIN 86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="28"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="265"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="501" mass="53947" checksum="6DC226682D9189E4" modified="2018-12-05" version="1">YRDEDDLSPPVSSPSAGPSPSPSASAESSDLLLLPWEILTHIASYLPAHCLINVLPKVCRTLSTVGEDATAWQLRAQRLIGSRVRFPVGPRDDFDWPTSCLEMEQLITFWRGQVAEGHENEGGEGVARVGVAAQEVAYGADEGIEVVMEVENDEIQPVLDGDQVEQLREGLEGRLEVDAAALIEDGRMEVEMVQDQPSRSPSPPPALECITLPSSHIALVNSVLLLGGKGAVCATGSRDYDVKLWDLEEGSNGALLHTLGRKGDFTSHQGWVWCLASEGPLLASGSFDSTVRLWDLQAGGTNRGLIRTGSAVLCLSCQNDVLLAGTFNKRINMYDTRAAEPLIKSVHLHGNAVMCLAADDTYIVSGSEDCTVAVYDCRAMKALKKIKLSSYLSSMSYSGYEVWAGDKKGMLHSFSMQTSTLKLLSRFDVGHTALVTGIHKSAGCLYTCSTDKTVKIHIPSGPPRTLCTLCHAGVVHGLSVEAGIVAVSTGDETVNIWRPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MN75</accession>
    <name>A0A3B5MN75_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 15">Membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MN75"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025006"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025310.1">
        <property type="protein sequence ID" value="ENSXCOP00000025006.1"/>
        <property type="gene ID" value="ENSXCOG00000018666.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282491"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015272">
        <property type="term" value="F:ATP-activated inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000036">
        <property type="entry name" value="ATP-sensitive inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000002">
        <property type="entry name" value="ATP-sensitive inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003268">
        <property type="entry name" value="K_chnl_inward-rec_Kir1.1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF6">
        <property type="entry name" value="ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005465">
        <property type="entry name" value="GIRK_kir"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01321">
        <property type="entry name" value="KIR11CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 15" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 15" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <keyword evidence="7 15" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="17">
        <location>
            <begin position="27"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="18">
        <location>
            <begin position="176"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="site" description="Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium" evidence="14">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR005465-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="384" mass="43642" checksum="D0832F0E93A5571C" modified="2018-12-05" version="1">VRKTLRLWKYREHMFPPGHRTRKSRLVTKDGHCNIEFGNIEYSNHFAFVVDFWTTFVEIRWRFVLVLFVAAFTGSWFMFSLLWYWIAKSNGDLTAQNQTEEHVRCIDNVNGLTTAFLYSLETQTTIGYGGRALTGHCAGTVALIIIQSLVGVFINCFMCGIILAKISLPKKRAKTVTFSNTAVICLKKGSLCLLIRVANLRKTLLIGSQIYGKLLRTTTTPDGETIILDQVDVDFMVDAGKDNLFFVCPLTLYHVINRSSPFYELSADTLPLQDFELVVFLDGTAESTSSSCQVRTSYIPQEIQWGYSFLPIISRTKTGKYRVDFSNFSKSVRVTTPHCVHCFETDVDHRMPNKENHINQLKLGIDNLGFQVINIPEPVDVTKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQU1</accession>
    <name>A0A3B5MQU1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fibronectin type-II domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQU1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021854"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022118.1">
        <property type="protein sequence ID" value="ENSXCOP00000021854.1"/>
        <property type="gene ID" value="ENSXCOG00000016328.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177582"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00062">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.10.10:FF:000001">
        <property type="entry name" value="Fibronectin 1a isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.10.10">
        <property type="entry name" value="Fibronectin, type II, collagen-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000562">
        <property type="entry name" value="FN_type2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036943">
        <property type="entry name" value="FN_type2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00040">
        <property type="entry name" value="fn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00059">
        <property type="entry name" value="FN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51092">
        <property type="entry name" value="FN2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-II" evidence="5">
        <location>
            <begin position="31"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="36"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="82" mass="9902" checksum="D29710B73008300E" modified="2018-12-05" version="1">EIRWTTYLITTTFFLLFFIHFSELYTIEGNAAGRPCMFPFFYENQWYSSCTLIDSINNQHWCSVETKFEHQTWGFCPTNSEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRB2</accession>
    <name>A0A3B5MRB2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Apolipoprotein F</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRB2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026328"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026647.1">
        <property type="protein sequence ID" value="ENSXCOP00000026328.1"/>
        <property type="gene ID" value="ENSXCOG00000019661.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00500000045104"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026114">
        <property type="entry name" value="APOF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15011">
        <property type="entry name" value="APOLIPOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15011:SF3">
        <property type="entry name" value="APOLIPOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15148">
        <property type="entry name" value="Apolipo_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017408709" evidence="1">
        <location>
            <begin position="22"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="323" mass="35075" checksum="4A86999111A86E76" modified="2018-12-05" version="1">MFSKLKWLFLIQLLLTEQSLCRVPPPPPKTDNLLQTTPASEDAEQKQDIQVLLGSSPQPDKSEPTSTRSQNVEKIRSAQLATWTISATLQGQIRDHLQIRGNVSCEELLYASAVDDPLSSVFPQELMGLSLVPVLVASGCPQEAQLLVLKLYDLLGKADTEELLMDLVALIERKMSRSTSASTSALFQSSGQRMKRSPQRSCINKDEERVYNVVEWIPAVSTLYSLGTAVYYASINCSATAKERAILSAVDLGTDALMVATGGTVGVAGYAVGAGVKTGVKAGVKYLMNSMKAEDDVLVNQSSLERGTFTAEKHVEKGFLFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSD8</accession>
    <name>A0A3B5MSD8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Solute carrier family 22 member 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Organic anion transporter 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Renal organic anion transporter 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Basal cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSD8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024111"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024397.1">
        <property type="protein sequence ID" value="ENSXCOP00000024111.1"/>
        <property type="gene ID" value="ENSXCOG00000017994.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165810"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009925">
        <property type="term" value="C:basal plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000023">
        <property type="entry name" value="Solute carrier family 22 member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="398"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="87"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="548" mass="60734" checksum="33C1A77209483BBD" modified="2018-12-05" version="1">MGFTDLLDQVGGFGRYQWIHVILISLPGLMTASQNLLNNFVSGIPVHHCKPATNQSLHDLGRTYQVTVSPPGSGRATQPIRRYVEPQWHMLSLNISLNASELQTEGCVDGWSFDQSEFSATTVSEWDLVCSLRPLKQMIQTIYMGGVLTGAIVFGGLSDRFGRKSVLIWSYLQLAVLGCSSALSPSYTAYCIFRFLSGMAMSGIILNGVSLKVEWIPTKTRTVVGTLTSFFFTFGQMILAGLAYWLRDWRKLQLVVCAPQFLFFTYSWWYVESARWLVLNRRSEEALKGLHRVARINRRPEAINKLTLEVLHSHMSKEIESSRSSFTAFDLVKTRRIRRISICLTAVWFSTSFAYYGLAMDLQKFGVSIYLMQIIFGAVDIPAKFLALGMLSYLGRRVSQVSCLFLSAVIIFANIFVPTDMQSLRTTLACLGKAFTSASFTTVYLYTGELYPTVIRQTGMGFVSTMARIGSMAAPAVLILDEVFPALPSMVYGGAAVLASGFACFLPETLNKPLPDTIEDVEENSVVTKEGVALKGLNEKEGSGLNAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSW7</accession>
    <name>A0A3B5MSW7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSW7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022594"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022870.1">
        <property type="protein sequence ID" value="ENSXCOP00000022594.1"/>
        <property type="gene ID" value="ENSXCOG00000016887.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286942"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007193">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000059">
        <property type="entry name" value="G protein-coupled receptor 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003909">
        <property type="entry name" value="GPR37_orph"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46216:SF2">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 37-LIKE 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46216">
        <property type="entry name" value="PROSAPOSIN RECEPTOR GPR37 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01421">
        <property type="entry name" value="GPR37ORPHANR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
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            <begin position="126"/>
            <end position="411"/>
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    <sequence length="473" mass="52782" checksum="EEB8928B5EA3E349" modified="2018-12-05" version="1">PRSASVESEVSSVEFIIGTPPVPDMVDLDRRVRRVRLARGADEDEQQSTFSHSFENEWVTMPQLTEFSNTTRVELNRRDEINLQANTSNPDDEGPLNPFFPLVESSYVAYVVLFLAGLVLAVGVVANMAVMCVVWNNYYMRSAWNYLLASIAFWDFLVLVVCLPVVVFNHLSHRRILGDITCRLVPYMEVASLGVTSFTLCALGIDRFHSATSSPQQKARRVERCRSVLLKMLLVWLSALLLSSPEIFLWQLSQAVSPSTGRMVDTCTITPSSPLSLYLPDSLHSLLLRYHQGRMWWCFGCYFCLPVLFTVLCQMATRNVNSDSSSAQKQRNHDDRANSQKRQQHQAVERQLNCTLLALAVVYGICALPEHVCNITLAYTHITVSEGTAAILVLLHHFLLFLKCAVTPVLLLCLCKALGQAFMDCCCCCCQECQPTTAQDSPGSAQVKLKAANETSIFFDKAKDTTAILSISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MT21</accession>
    <name>A0A3B5MT21_9TELE</name>
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        <recommendedName>
            <fullName evidence="10">E3 ubiquitin/ISG15 ligase TRIM25-like</fullName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    <sequence length="237" mass="26996" checksum="6DF2C3F1EBCF5885" modified="2018-12-05" version="1">MAHIGHNHLAQQTLCCSICLDLLKDPVAIACGHNYCMNCIKTHWDEEDEKGIHSCPQCRETFTPRPALKKNTMLAALVEQMKKTGLQAAPADHRYAGPEDVGCDFCTERKLKAIKSCLVCLASYCEKHLQPHYDSDTFKKHKLVEPSKNLQENICSKHDKVTDIFCRTDQKCICYLCSVDEHKGHDTVSAAAEIAERQRELEERRGNIQQRIQDREKDVKLLQQEVESSVNPTRFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MTK8</accession>
    <name>A0A3B5MTK8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Calcium and integrin-binding family member 2</fullName>
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    </protein>
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        <name evidence="16" type="primary">CIB2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Acanthomorphata</taxon>
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            <taxon>Atherinomorphae</taxon>
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            <taxon>Poeciliinae</taxon>
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    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <location evidence="1">Sarcolemma</location>
        </subcellularLocation>
        <subcellularLocation>
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            <location evidence="4">Cilium</location>
            <location evidence="4">Photoreceptor outer segment</location>
        </subcellularLocation>
        <subcellularLocation>
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            <location evidence="5">Stereocilium</location>
        </subcellularLocation>
        <subcellularLocation>
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        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Photoreceptor inner segment</location>
        </subcellularLocation>
    </comment>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45791">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
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            <begin position="144"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00004645"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="187" mass="21900" checksum="178C40C8C054AB62" modified="2018-12-05" version="1">MGNKQTIFTDEQLDAYQDCTFFTRKEILRLHSRYHELAPHLVPMDYTNEPDCKLPLALIVDMPELKENPFRNRIVESFSEDGMGNLSFNEFVDMFSVLSEMAPRELKAIYAFKIYDYNVDNYLCKEDLEKTLNKLTKEELTPEEVELVCEKAIEEADLDGDNKLSYADFENMITRAPDFLSTFHIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVF4</accession>
    <name>A0A3B5MVF4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027868538.1">
        <property type="nucleotide sequence ID" value="XM_028012737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVF4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027803"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028138.1">
        <property type="protein sequence ID" value="ENSXCOP00000027803.1"/>
        <property type="gene ID" value="ENSXCOG00000020731.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114141863"/>
    <dbReference type="KEGG" id="xco:114141863"/>
    <dbReference type="GeneTree" id="ENSGT00940000159088"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903531">
        <property type="term" value="P:negative regulation of secretion by cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08404">
        <property type="entry name" value="C2B_Synaptotagmin-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000039">
        <property type="entry name" value="Synaptotagmin 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000051">
        <property type="entry name" value="Synaptotagmin 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF115">
        <property type="entry name" value="SYNAPTOTAGMIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="16"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="195"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="328"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="167"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="167"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="468" mass="51559" checksum="A7F38DEFF387C3D1" modified="2018-12-05" version="1">MADIIELGPAYAMSPVLAGFLGAGVLVLVVVVLVLLWSFCQRRYLRVTGRYKLNGDRYCDAEDPPYKFIHMLKGISIYPESLSGSKRIVRGIRRADRSERDGERGGSAGGAAGRGMVLVDAENNILDVPGQLQMSHLVPPTGSGPAHSQARLERALPVRADYCCLDSSSAGSSQTSSKTASPFTPASSEPEPEPNLGSVSLSVDYNFPKKALVVTVVGARGLPAVDEQAGSSDPYVKMTILPEKKHRVKTRVLRKTLDPIFDETFTFYGVAYSALPDLTLHFLVLSFDRFARDDVIGEAVVPLKGVDPSTGRVHLSQQITKRNMQCESRGELLASLSYQPVSHRLSVVVLKARHLPKMDMTGLSANPYVKVNVFYGRKRIAKKKTHVKKCTLNPVFNESFIYDIPPELLPDVSVEFLVVDFDRTTKNEVLGRLLLGLHSPCPSGASHWREVCENPRRQISKWHNLSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVY4</accession>
    <name>A0A3B5MVY4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal fusion of the chorion and the allantois during placenta development. Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
            <location evidence="1 11">Extracellular space</location>
            <location evidence="1 11">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVY4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000028003"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028344.1">
        <property type="protein sequence ID" value="ENSXCOP00000028003.1"/>
        <property type="gene ID" value="ENSXCOG00000020887.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158861"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048797">
        <property type="term" value="P:swim bladder formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000001">
        <property type="entry name" value="Wnt homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013300">
        <property type="entry name" value="Wnt7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF73">
        <property type="entry name" value="PROTEIN WNT-7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01891">
        <property type="entry name" value="WNT7PROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0879">Wnt signaling pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000028003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="358" mass="40386" checksum="92B44CA226725A59" modified="2018-12-05" version="1">MCDAVGQCFSQKKLETCQTAWNLEPFKGALYIMALSSVVALGANIICNKIPGLAPRQRAICQSRPDAIIVVGEGAQMGINECQYQFRYGRWNCSALGERTVFGQELRVGSREAAFTYAITAAGVAHAITAACSQGNLSQCGCDREKQGYYNQEEGWKWGGCSADIKYGIEFSRRFVDAREIRKTPRRLMNLHNNEAGRKVLEERMKLECKCHGVSGSCTTKTCWTTLPKFREIGYILKEKYNDAVHVEVVRASRLRQPTHLKVKQTQGYRKPMETDLVYIERSPNYCEEDTATGSVGTQGRLCNRTSPHTDGCDLMCCGRGYNTHQYTKVWQCNCKFQWCCFVKCNTCSERTEVFTCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWM5</accession>
    <name>A0A3B5MWM5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier organic anion transporter family member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the organo anion transporter (TC 2.A.60) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWM5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024139"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024428.1">
        <property type="protein sequence ID" value="ENSXCOP00000024139.1"/>
        <property type="gene ID" value="ENSXCOG00000018023.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286901"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015347">
        <property type="term" value="F:sodium-independent organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015732">
        <property type="term" value="P:prostaglandin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043252">
        <property type="term" value="P:sodium-independent organic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004156">
        <property type="entry name" value="OATP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00805">
        <property type="entry name" value="oat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388">
        <property type="entry name" value="ORGANIC ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388:SF86">
        <property type="entry name" value="SOLUTE CARRIER ORGANIC ANION TRANSPORTER FAMILY MEMBER 3A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03137">
        <property type="entry name" value="OATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Kazal-like" evidence="10">
        <location>
            <begin position="457"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="700" mass="75825" checksum="92EAED0483356249" modified="2018-12-05" version="1">MQVKSQICTERSSTDDPEQDDNQKKTSCFSNIKIFLVAECALMLAQGTVGAYLVSVLTTLERRFNLQSADVGVIASSFEIGNLALILFVSYFGAKAHRPRLIGCGGIVMALGALLSALPEFLTHQYEYEAGDSWHAEDGRVICSNKTRSENRDSGFNCGNRANTNMMYLLLIGAQVLLGIGATPVQPLGVSYIDDHVHKKDSSLYIGILFSTLVFGPACGFILGSICTKVYVDAVFIDTSKLDITPDDPRWIGAWWGGFLLCSALLFLSALFMFGFPQALDEQDMDTRGESEQAMLPTSLSLESQDSKPNGDFRGFDMNSGLSVWEHLRVIPRVTRHLLSNPVFSCITLAACMEIAVVAGFAAFLGKYLEQQFNLTTSSANQLLGMTAIPCACLGIFLGGLLVKKLNLSALGAVRMAMLVNLVSTACYVSFLFLGCDTGPVAGVTVAYNNETLQSKKQPESGCNSNCNCYTASVSPVCGSNGVTYISACFAGCTKPNLSSCGCIPSIGGENEASPGKCSSPRCQHAFLTFLSVICVCSMIGAMAQTPSVIILIRTVSPELKSYALGVLFLLLRLIGFIPPPLIFGMGIDSTCLSWSSVCGEKGACMLYDNVAYRHLYVSIAIVLKSSAFLLYATTWQCLKSNYRRYIKNNEGYLTPTELFASNVTLDNLGKEITQNPSNRTKFIYNLEDQETSDNMESVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MYS1</accession>
    <name>A0A3B5MYS1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Gamma tubulin complex component protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MYS1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027167"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027500.1">
        <property type="protein sequence ID" value="ENSXCOP00000027167.1"/>
        <property type="gene ID" value="ENSXCOG00000020283.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157872"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF14">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="6">
        <location>
            <begin position="242"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="208"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="353" mass="39033" checksum="B646D13128A929EE" modified="2018-12-05" version="1">MANLDQKSPNVLLQSLCCRITGKSEAEVAHQFQYAVRVIGSNYAPTIERDEFLVSEKIKKELLRQRREADAALFSELHRKLQTQGVLKHRWSVLYLLLSLSEDPRKPSSRVGNYGALFAQALPRDAHSTPFYCTRPQSLALSYGDKGAGLSGSVGTSGISSLGVYTLNGPTPTPPQLLSAAAVTQSLGSRLAWALPISSSPSAVLAPPITRPPLGPQVPTIRPVRPRRGGDATGEVNEAALVRDILYVFQGIDGKFIKMNAQDNGYKMDSKVVLCKSLRDTSSRLSELGWLHNKVRKYTDARTLDRAFGLVGQSFCASLHQELKEYYRLLSVLHSQESWPLRFTPTGRRETRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZM8</accession>
    <name>A0A3B5MZM8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calbindin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MZM8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027682"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028017.1">
        <property type="protein sequence ID" value="ENSXCOP00000027682.1"/>
        <property type="gene ID" value="ENSXCOG00000020640.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183108"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900271">
        <property type="term" value="P:regulation of long-term synaptic potentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099509">
        <property type="term" value="P:regulation of presynaptic cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000054">
        <property type="entry name" value="Calbindin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000116">
        <property type="entry name" value="calretinin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051001">
        <property type="entry name" value="Calbindin_Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19972">
        <property type="entry name" value="CALBINDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19972:SF4">
        <property type="entry name" value="CALRETININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="16"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="63"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="107"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="266"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="285" mass="33052" checksum="9C850F7E5EC74768" modified="2018-12-05" version="1">MANKAQQPPHLHLAEVTASQFLDIWKHFDVDGNGYIEGKELENFFRELETARRGAGVDPTNPTFRERMKEFMQKFDKNKDGRIEMSELAQILPTEENFLLCFRQFVSSSAEFMAAWRRYDTDRSGYIEANELKGFLSDLLEKANRHYDDQKLQEYTQTILRMFDLNGDGKLVLLNSIPNIKMFFVIFYIFVFWVLLQGVKLTSEQFNSIFNYYDKVGGICFQVPLIGFIRGDEVENVETAIASLFKGLAGFALNRSWFDVVCQRQTSSNTDLSPGPKPYKHHRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N0J6</accession>
    <name>A0A3B5N0J6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5N0J6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026680"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027007.1">
        <property type="protein sequence ID" value="ENSXCOP00000026680.1"/>
        <property type="gene ID" value="ENSXCOG00000019929.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154089"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14089">
        <property type="entry name" value="STKc_MAPKAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000094">
        <property type="entry name" value="MAP kinase-activated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000156">
        <property type="entry name" value="MAP kinase-activated protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1170.10:FF:000001">
        <property type="entry name" value="MAP kinase-activated protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1170.10">
        <property type="entry name" value="MAP kinase activated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027442">
        <property type="entry name" value="MAPKAPK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="64"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="381"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="390"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="417" mass="46998" checksum="EF4D96C69AB23935" modified="2018-12-05" version="1">MLHIEEEEEEEEQEGTPSPGQHGTALRSSGSSSLGSSSSSTAGLLPPAYPKVEFRRNAVTDDYKITSQVLGLGINGKVLECYCKKTGEKCALKILYDTPKARREVDLHWRVSGGPHIVRILSLYENMHQGKKCLLIVMECMEGGELFSRIQVRGDQAFTEREVSEIMHDIGTAIAYLHHVDIAHRDVKPENLLYTTKDSNATLKLTDFGFAKETKMHNSLQTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGFPPFYSNTGQAISPGMKQRIRLGQYEFPNPEWVDVSEEAKQLIVQLLKTDPSERMTIGQFMNHPWISQSMAVPQTPLHTSRVLTEDMELWDDVKEGMTSALATMRVDYDQVKIKDLDTSNNPLLNKRRRKERLEAAELSRRPSGSDGAQSDLPVSYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N0M0</accession>
    <name>A0A3B5N0M0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RAN binding protein 9</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RANBP9/10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N0M0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026735"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027062.1">
        <property type="protein sequence ID" value="ENSXCOP00000026735.1"/>
        <property type="gene ID" value="ENSXCOG00000019969.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157305"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd12909">
        <property type="entry name" value="SPRY_RanBP9_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000011">
        <property type="entry name" value="RAN binding protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035782">
        <property type="entry name" value="SPRY_RanBP9/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LisH"/>
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    </dbReference>
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        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="3">
        <location>
            <begin position="90"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="CTLH" evidence="4">
        <location>
            <begin position="346"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="404"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="639" mass="70390" checksum="73AC5E8647E6D981" modified="2018-12-05" version="1">MFIPALAEWISISISVIVDRKKTKFPQKNSDILSIRLGSNLFFKNSGLLLTSHFTKSRVVAMSGQSTGCGFLMSVVVHGDSALNEQEKELNQRLRRLYPAVNENETPLPRSWSPKDKFSYIGLSQNNLRVHYKGHGKTPKDAASVRATHPIPAACGVYYFEVKIISKGRDGYMGIGLSAQGVNMNRLPGWDKHSYGYHGDDGHSFCSSGTGQPYGPTFTTGDVIGCCVNLINNTCFYTKNGHSLGIAFTDLPPNLYPTVGLQTPGEVVDANFGQHPFVFDIEDYMREWRTKIQAQIDRCPIGEREGEWQSMIQKMVASYLVHHSYCATAEAFAKSTDQAVHEELASIKNRQKIQKLVLSGRMGEAIETTQQLYPNLLERNPDLLFMLKVRQFIEMVNGTDSEVRCLGGRSPKSQDSYSGSPRPFSSPSHKASSSQPYLTGTPPMSGCFLVLWNAASPFSLHFLFPLSNDVDMEVDHFTNGVTESSSNGFLNGTSKHATEPDDCDADMGGFDSAQSKRQLCGGSQAAIERMIHFGRELQSMSEHLRRECGKNSANKKMLKDAFSLLAYSDPWSSPVGYQLDAVQREPVCSTLNSAILETHNLPKQPPLAQAVGQAAQCLAIMARTGIGSCAFASVDDYLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N0R3</accession>
    <name>A0A3B5N0R3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5N0R3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027164.1">
        <property type="protein sequence ID" value="ENSXCOP00000026835.1"/>
        <property type="gene ID" value="ENSXCOG00000020037.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160977"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="hematopoietic SH2 domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14388:SF6">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14388">
        <property type="entry name" value="T CELL-SPECIFIC ADAPTER PROTEIN TSAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="4">
        <location>
            <begin position="60"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="167"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="300"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="387"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="258"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="352"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="435" mass="49149" checksum="21714C44B50C6191" modified="2018-12-05" version="1">SPPLGLPTVLYRDKVKMEQREPTVDPAAEVTEGRFRELATKWFIDTQLPLIVNNGLFPSWFLGFISRKDAEDMLREKDLGCFLIRLSDKAIGYILSYKGRDRCRHFVISQSETGRFIVCGDTEEHNTLFDLTEHYKTKPVEPFGEYLTSPCYGDLYDIIQVSPKEKPAAPLRAAKKVHKQQMNSATEQQPVRAPRINRTQEVIPPLPRRTRHFESSPVSDNDGVLYAQLRKQTPRAHHASRDKSPGSNQGKVERLNTKEQNNGRCSPSSDPESLYSELNALDSKSMSLPLLDSISDGEQCYRLSVPPDTPPRLSPRPVRQASRGISQSNSSHSLDGLSDHSVYHLAGSHSPTASETASSTEQLDDSLYAEVSIEAPVIDNDNTYEILPNHKEPVKPKAKRNTQEPGEETKRKFNNGSWGIKNDKWKWLFPETKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N1A5</accession>
    <name>A0A3B5N1A5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">F-box/LRR-repeat protein 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">F-box and leucine-rich repeat protein 14</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="6">Part of a SCF (SKP1-cullin-F-box) ubiquitin-protein ligase complex. Interacts with SKP1 and CUL1. Interacts with SNAI1; the interaction requires the phosphorylation of the two serine residues in the substrate destruction motif D-S-G-X(2,3,4)-S.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027857282.1">
        <property type="nucleotide sequence ID" value="XM_028001481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5N1A5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027355"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027688.1">
        <property type="protein sequence ID" value="ENSXCOP00000027355.1"/>
        <property type="gene ID" value="ENSXCOG00000020427.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114134715"/>
    <dbReference type="KEGG" id="xco:114134715"/>
    <dbReference type="GeneTree" id="ENSGT00940000159857"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22125">
        <property type="entry name" value="F-box_FBXL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000051">
        <property type="entry name" value="F-box and leucine-rich repeat protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000075">
        <property type="entry name" value="F-box/LRR-repeat protein 14 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000059">
        <property type="entry name" value="Partner of Paired"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050648">
        <property type="entry name" value="F-box_LRR-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047932">
        <property type="entry name" value="FBXL14_F-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13382">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE COUPLING FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="9">
        <location>
            <begin position="13"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="10">
        <location>
            <begin position="121"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="10">
        <location>
            <begin position="203"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00065882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00080419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="403" mass="44651" checksum="385F1A560E0D24F3" modified="2018-12-05" version="1">MFQTETHISCLFPEILAIIFSYLDVKDKGRVAQVCAAWRDASYHKSVWRGVEAKLHLRRANPSLFPSLQTRGIKKVQILSLRRSLSYVIQGMPQIESLNLCGCFNLTDNGLGHAFVQDIPSLRVLNLSLCKQITDSSLGRIAQYLKNLEVLELGGCSNITNTGLLLIAWGLHRLKSLNLRSCRHVSDVGIGHLSGMTRSAAEGCLTLEKLTLQDCQKLTDLSLKHVSKGLNKLKVLNLSFCGGISDAGMIHLSQMTQLCSLNLRSCDNISDTGIMHLAMGSLRLSGLDVSFCDKIGDQSLAYIAQGLYQLKSLSLCSCHISDDGINRMVRQMHELKTLNIGQCVRITDKGLELIADHLTQLTGIDLYGCTKITKRGLERITQLPCLKVLNLGLWQMTESERVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N1B8</accession>
    <name>A0A3B5N1B8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Myosin, light polypeptide 3, skeletal muscle</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="32473.ENSXCOP00000027385"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027718.1">
        <property type="protein sequence ID" value="ENSXCOP00000027385.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043292">
        <property type="term" value="C:contractile muscle fiber"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:myosin II complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="CALM/Myosin/TropC-like"/>
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        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
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    </dbReference>
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        <property type="entry name" value="MYOSIN LIGHT CHAIN 1_3, SKELETAL MUSCLE ISOFORM"/>
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    </dbReference>
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        <property type="entry name" value="EF_HAND_2"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="120"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="187" mass="20467" checksum="AA2C22712A2C8CF5" modified="2018-12-05" version="1">AFPKKVKDVPTCHLIPPPSPGAGALTMAQDHSVSGLQLLFSFLFAPPYFKEAFGLFDRVGDSQVAYNQVADIMRALGQNPTNKDVVKILGNPSADDMANKRLNFDAFLPMLKQVDTLPKGTYDDYVEGLRVFDKEGNGTVMGAELRIVLSTLGEKMNETEIDALMAGQEDENGSVHYEAFVKHIMSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KJS3</accession>
    <name>A0A3B5KJS3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alkylated DNA repair protein AlkB homologue 8 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000000418"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000426.1">
        <property type="protein sequence ID" value="ENSXCOP00000000418.1"/>
        <property type="gene ID" value="ENSXCOG00000000373.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000235216"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1820:FF:000004">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015095">
        <property type="entry name" value="AlkB_hom8_N"/>
    </dbReference>
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        <property type="entry name" value="Transposase_22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11505">
        <property type="entry name" value="L1 TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09004">
        <property type="entry name" value="ALKBH8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alkylated DNA repair protein AlkB homologue 8 N-terminal" evidence="3">
        <location>
            <begin position="322"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="384"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="99"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="396"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="407" mass="46053" checksum="75990EF25798126E" modified="2018-12-05" version="1">MGGNKYAHSCPTPLTVGNSRVAVCPAPPKEAGSRTRAVCRGETDCQPHTNDVAEDMEQGSIESDVEEANTLKEDIIVERVTKNIEEILDRKVAAILKPVSELAEKFDSMTRRMAAAEQRISDLEDVTTKSAPRMDGMEKTIERALERLDSLENQSRRQNIKIMGLKEGREPAAFFEEWIPKVLGLQQTPIKIERAHRTGPPAGRSDRDHPRAVLVRLHNYIDKQKILNAARNKGIIQVEDRRVSFYQDFSAEIVRKRQESSGLVDWCRDNSLILNVDKTKEMIVDFRKNRPSHAPLLINSSAVEVVSSTKFLGVHITNDLTWTVNTTSLVKRAQKRLYFLRRMRRAHXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRTACVHQSDQRPLQKQEVNYSAGHSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQ64</accession>
    <name>A0A3B5KQ64_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQ64"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002278"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002312.1">
        <property type="protein sequence ID" value="ENSXCOP00000002278.1"/>
        <property type="gene ID" value="ENSXCOG00000001822.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158902"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034458">
        <property type="term" value="F:3'-5' RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000615">
        <property type="entry name" value="pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000009">
        <property type="entry name" value="Probable ATP-dependent RNA helicase DHX40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000145">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF271">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX40-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="27"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="225"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="742" mass="84055" checksum="72F28C3945A6EAAC" modified="2018-12-05" version="1">MSKQTRWTPKDCEPKQLPIYQHKNKLIQAVRSSTFLIVTGETGSGKTTQLPQYLHQAGFCKDGKIGVTQPRRVAAITVAQRVAQEMKCTLGREIGYQVRFDDCTSQDTVLKYMTDGCLLREVLADPVLSQYSVVILDEVHERSLNTDILLGLLKKVFSNPREAMKGRSFPLKVVVMSATLETDKLSSFFNNCPVFEIPGRVFPVASMFGTAVGPKDVESTFYVKEVVKVALDVHTSEEAGDILVFLTGQSEIEHACDMLFEKAENIDYRYDVQDRTVEGLLILPLYGSMPTDQQRQIFQAPPPGIRKCVVATNIAATSLTINGIKYIVDSGFVKQLNHNSRVGMDVLEVVPISKSEAQQRAGRAGRTSAGKCFRIYTKGFWEKCMPEYTIPEIQRTSLTAVILTLKCLGVHDVIRFPYLDCPEERFILEALKQLYQFDAIDRRGRVTKLGELMVEFPLHPGLTRALLKAASLGCQDLLLPVAAMLSVENIFIRPGQPDKQKEAAKQHRELAAKTGSMNDFATLLSVFNACKSSDRPSGWCKENWIHWRALKSAFSVETQLREILLRLQQRRDFPFETFDGNKSELFRRCLCAGYFTNVARRSVGKVFCTMDGHGSMVHIHPSSSLFEQDVELNWVIFHDVLVTSKMYIRTVCLIRYEWVKDLLPKLHEVDVYELSSVARQEVTEDEMVKWESREAAKRQQEASAEDVMKKLEKRNNEATVSEARAPKQPVFIWCNLHFLSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQG3</accession>
    <name>A0A3B5KQG3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pirin</fullName>
            <ecNumber evidence="8">1.13.11.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Probable quercetin 2,3-dioxygenase PIR</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Transcriptional coregulator of NF-kappa-B which facilitates binding of NF-kappa-B proteins to target kappa-B genes in a redox-state-dependent manner. May be required for efficient terminal myeloid maturation of hematopoietic cells. Has quercetin 2,3-dioxygenase activity (in vitro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>quercetin + O2 = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO</text>
            <dbReference type="Rhea" id="RHEA:15381"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:57628"/>
            <dbReference type="ChEBI" id="CHEBI:57694"/>
            <dbReference type="EC" id="1.13.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="11">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Flavonoid metabolism; quercetin degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">May interact with NF1/CTF1. Interacts with BCL3. Identified in a complex comprised of PIR, BLC3, NFKB1 and target DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the pirin family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.24" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQG3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000712"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000722.1">
        <property type="protein sequence ID" value="ENSXCOP00000000712.1"/>
        <property type="gene ID" value="ENSXCOG00000000598.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008044"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008127">
        <property type="term" value="F:quercetin 2,3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030224">
        <property type="term" value="P:monocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02247">
        <property type="entry name" value="cupin_pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02909">
        <property type="entry name" value="cupin_pirin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000055">
        <property type="entry name" value="pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903:SF8">
        <property type="entry name" value="PIRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin N-terminal" evidence="13">
        <location>
            <begin position="21"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin C-terminal" evidence="14">
        <location>
            <begin position="191"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR006232-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="275" mass="30515" checksum="4EE4AC5FDC2B985D" modified="2018-12-05" version="1">MIVRKLLKTVLSVEQAEGVGARVRRSIGRKELSNLDPFLMLDEFKVVKPAGFPDHPHRGFETVTYVLGGITAHEDFCGHSGRLMPGDLQWMTAGRGVVHAEMPVSEEPVVGLQLWVNLRKQEKMIEPAYQELKSSEIPKRSQGGVTVAVISGEALGAKSKVYTRTPTLYLDFKLQAGAMHVQPVPSGSDQDQQPVGPHHTVVFGDGDCVRFENKGSEVSHFVLIAGEPINEPVVQQGPFVMTTQEEIHQAKMDFYNGTNGFERAKHWRSKIRDSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQQ6</accession>
    <name>A0A3B5KQQ6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the interleukin-1 receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQQ6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000807"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000817.1">
        <property type="protein sequence ID" value="ENSXCOP00000000807.1"/>
        <property type="gene ID" value="ENSXCOG00000000685.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01090000259985"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015621">
        <property type="entry name" value="IL-1_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890:SF6">
        <property type="entry name" value="INTERLEUKIN-18 RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017486544" evidence="5">
        <location>
            <begin position="19"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="6"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="6">
        <location>
            <begin position="282"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="440" mass="48974" checksum="D834534DB5E8E639" modified="2018-12-05" version="1">IQVLLPTVLLLSICPVTAKEIYANAGDLVMLNCRESKHDGTVMLWKMEKDQEMHLYSNMSASEQQKLGLIFYRRSLVILSASTNHQGNYSCGPLGYELMLTLHTLRVSLNKYPMRCFAKQACKLICPEGSIPDAGIPGMQSMEHVWHKNGEPSVNYFPSVGVENSGVYICMRSFLYSGEIYNTSSSLELDVQTGLIDVPFVLSISENEKMPKWMNTSLIFREVSPENLSKTFTCAYSSHKLISTVNITLVKIVIVFNLHQNGFLKANMGEVNNGFFVFLDGRSYDAFLMCYKSITDGGLSEEDRKCLASTLENEFGYSICLYDRDVLPGQAVADAVLDCIQQSRAVVLIPGFPDPEPGSAVLSAIHSSLVERKTRLIFINTEQTEASTSGSFPEALQLLSKAGNSVTWKGRPPPTSFWKQLRYHLPAPQQTPKIPNTHPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQZ0</accession>
    <name>A0A3B5KQZ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQZ0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002572.1">
        <property type="protein sequence ID" value="ENSXCOP00000002538.1"/>
        <property type="gene ID" value="ENSXCOG00000002024.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286915"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001524">
        <property type="entry name" value="Si:dkey-126g1.7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT" evidence="7">
        <location>
            <begin position="136"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="640" mass="74296" checksum="53413AD3E9883F6D" modified="2018-12-05" version="1">MTVNTKEIGNPEMSPLRVTHQGQMLFKCDALKPSTLKLSFFLYLLESFITCQQTLKSNLKKKLQFVFEGIAKSGNQTFLNQIYTELYITEGDSGDVNKEHEVRQIESVSWKPDRPETTIRHEDIFKHSPDRDEPIRAVMTKGVAGIGKTVLAQKFTLDWAEDQANQDIQFTFPFTFRELNLLKDQQFSLVELVHHFFSETRGISTFEELKVVFIFDGLDESRLPLDFNNNEILTDVRKSTSVDVLLTNLIRGNLLPKAHLWITTRPAAASRIPPEFIGMMTEIRGFTDSQKDEYFTKKFRYKKQARMVISHVKESRTLHIMCHIPIFCWITAKVVEDMLNKKDGEELPKTLTEMYIHFLVVQTKLKVIKYDGGAETDSHWSPKSRKMIESLGKLAFDQLMKRNLIFYESDLAECGIDARAASTYSGVFTQIFREERGLYQNKVFCFVHLSVQEFLAALHELEKRSHEFPETNIYKNGHLDLFLRFLLGLSLETNRRHLRGFVAQTEESLYRAFIKAFKQGVEIKTTNADCLHYQEQFTKGQPRELVRSCQHMDPDYGYTVAKTLLQEHFRNPYKAATAYINKALSWPTIRTEDIRALQSYSLFLCGCCNVMEQLEYVQELDMPINMRAILAKLPYKLREC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRL7</accession>
    <name>A0A3B5KRL7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="18">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17 18">Belongs to the peptidase C12 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRL7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000286"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000293.1">
        <property type="protein sequence ID" value="ENSXCOP00000000286.1"/>
        <property type="gene ID" value="ENSXCOG00000000262.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157306"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09616">
        <property type="entry name" value="Peptidase_C12_UCH_L1_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057254">
        <property type="entry name" value="UCH_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF19">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE ISOZYME L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00707">
        <property type="entry name" value="UBCTHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00140">
        <property type="entry name" value="UCH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 17 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0636">Prenylation</keyword>
    <keyword evidence="7 17 18" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 17 18" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="8 17 18" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="19">
        <location>
            <begin position="2"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="17">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="17">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="17">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="17">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="200" mass="22209" checksum="C6EB7A98266B352F" modified="2018-12-05" version="1">MEWTAMEINPEIMRKLGVSGRWRFVDILGLEGEQLTAVPKPSCALMLLFPLTQQHEAFRTQQANKISGGSDVYFLKQVASNSCGTIALVHAIANNKAKMTFENGSVLKTFLDETAGMSPDERSKHLEGNKPEGEQVNFHFIAFVNVNGQLYEFDGKMNGPVNHGATKDETFVTDAAKVCRSFMEREKDEVRFSAVALCQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KS41</accession>
    <name>A0A3B5KS41_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Androgen receptor alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the nuclear hormone receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KS41"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000735"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000745.1">
        <property type="protein sequence ID" value="ENSXCOP00000000735.1"/>
        <property type="gene ID" value="ENSXCOG00000000614.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155516"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042562">
        <property type="term" value="F:hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033993">
        <property type="term" value="P:response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07173">
        <property type="entry name" value="NR_DBD_AR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
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        <source>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="392" mass="44098" checksum="97F826C95C2433FA" modified="2018-12-05" version="1">MPRFAAGELRGSVQFGGHGRCAVCDARLGHLQICLQGLEHVHELDSVFLSDRRVCQVCGDDASGCHYGAVTCGSCKVFFKRAAAGKQNHLCASRNDCTIDKLRRKNCASCRLKRCFMSGMSLKGTQLAGPQLLGIPPTMRSCLSLLTILQSIEPAVVNAGHDPAQPDSPASLLTSLNELGERQLVTVVRWAKAIPGFRDLHVDDQMSVIQLSWMGVMVFALGWRSYTLTNCSMLYFAPDLVFNDQRMQVSSMYEHCVRMKLLAQRFCKLEVTEEEFLCMKALVLFSIMPVEGLRSQRCFDELRTSYIKELDRLASHHGETTRTQRLFQLTQLLDYLQSVVRKLHQFTYDLFIQAQSLQMRVNFPEMISEIVSVHVPKILSGMVKPILFHNTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KSV3</accession>
    <name>A0A3B5KSV3_9TELE</name>
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        <submittedName>
            <fullName evidence="21">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
    </comment>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
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    </dbReference>
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        <property type="entry name" value="Hormone receptor domain"/>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
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        <property type="entry name" value="IG_LIKE"/>
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    </dbReference>
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        <property type="entry name" value="LRR"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017477912" evidence="16">
        <location>
            <begin position="22"/>
            <end position="1303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="724"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="839"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="879"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="960"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="987"/>
            <end position="1008"/>
        </location>
    </feature>
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        <location>
            <begin position="225"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="18">
        <location>
            <begin position="306"/>
            <end position="401"/>
        </location>
    </feature>
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        <location>
            <begin position="551"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="19">
        <location>
            <begin position="721"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1060"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1142"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1213"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1061"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1142"/>
            <end position="1153"/>
        </location>
    </feature>
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        <location>
            <begin position="1154"/>
            <end position="1164"/>
        </location>
    </feature>
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            <begin position="1218"/>
            <end position="1230"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00023319"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
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    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1303" mass="144541" checksum="9492BC2D85FB517B" modified="2018-12-05" version="1">MRLDWLQLVVVLLLSGLRGSAVPFACKTSDEGPKSGGKSPASDRKVVCSNMELHQVLPADSFPNRTVILILNNNKIQELRNGAFSGLSTLEKLDLRNNLISHIEPGAFLGLKTLKKLDLSNNSISCLNGDVFRGLESLSRLILSGNMFSSLAQGMFDRLVSLKALEFQTPYLLCDCNLLWLLRWIKDRNVSVKNTKCSYPQSLQGQLITSLRPELLTCDAPLELPSFQLTPSQRQVVFQGDSLPFQCQASLVAEDMQVLWYQNGRMVKPDATQGIFIEKRIVQNCSLIASALTISNIQPGFTGNWECRVRTSRGNTTRAVHIVVLQSSAKYCAPERISNNKGEFRWPRTLAGIKAYLPCNLPTSSARSYSGILSEERQAWRHCSPEGLWTEGDYSQCQFQKEVTRGLYVINQMPLNETNVVIKAQSLLVYTIDAANLSDKMDIIFMAEMIEKLGEFVEKFKDLGEVMVNMASNLMLADERVLWMAQREAMACSRIIASLQKISAYRLSTAQAFSLTSPNIALEAHTVRVNDWNGMTCILFQRPSPERLPGHDRQLTFKCNTTSSFASGLYKSTIVEASLQLPRSLFSQAATPGQTDDNVYKLYLLGFRNGKFFPSTWNTSLLADGGRKRSVATPVIMAKMDGISLRSLKTPINITLRRFARGSDAVSARWNFSLAGDQGGWRSEGCRILENHDNFTTMSCNSLGNYGLLMVGGFSPTIQPLHPVIYATSVILLLCLFTIIISYIYYYRSVCVSRKFWHMLVNLCFHMALTCGTFVGGINQTRYASVCQAVGILLHYSTLATALWVGVTARNIYKQLTRKAKRYEELDEPPPPPRPMLRFYLIGGGIPIIVCGITAAANIKNYGSQRYSPYCWMAWEPSIGAFYGPAGFIVFVDCMYFLSILLQVHRHPERRYELKESSEEQQHLASAGSEPNEALSSVGSAPHAVPLSALENEHTFTAQLIGAVGALGLYSGLWVFGAMAVSQDHPFDMAFTCLFGLTALALGAFMMAHHCVNRQDMRRFWLQVYCSRRRVYSAQEEVLLPQTGVAVMVTAEKADGKSVKCSHSSADSSYTNRSAPTSIRNSGHGSKLTNLHAEAAQCKAASAPATANGTAVLDSSLTEHSLDNEIKMHVAPVEVQFRPLSNISNPAANGSTSRHQKNRARAHRASRLTVLREYAYDVPTSVEGSVQSAPSRRHHYYDMAARNSRRAAYMAYRERHQSQLQQDSSDSASLPRRCRFSEKGTASKDTVLLPGSSKSENKSSKWYGLNLVTQNNGTLRKNGQAMPLVNGDSACIKTGLWKHETTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTX2</accession>
    <name>A0A3B5KTX2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CUB and Sushi multiple domains 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTX2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001947"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001975.1">
        <property type="protein sequence ID" value="ENSXCOP00000001947.1"/>
        <property type="gene ID" value="ENSXCOG00000001563.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161110"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000002">
        <property type="entry name" value="CUB and Sushi multiple domains 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24255">
        <property type="entry name" value="COMPLEMENT COMPONENT 1, S SUBCOMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24255:SF31">
        <property type="entry name" value="CUBILIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017429205" description="CUB and Sushi multiple domains 2" evidence="4">
        <location>
            <begin position="18"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="22"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="6">
        <location>
            <begin position="125"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="5">
        <location>
            <begin position="182"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="243" mass="27326" checksum="636663E11703321F" modified="2018-12-05" version="1">TLFQHFGLMLFKQFITAFCSQCGGIREEMEGVILSPGFPGNYPSNSDCTWRIYLPVGYGEHHQRLSKHNKLTFYVPIRNGPLDTSAVIGRFSGQDVPPPLLSTSHETTVYFHSDHSQNKPAYELQECPDPEPFRYGVVVGAGYNVGQSISFECLPGYQLMGHSILTCEHGTTRNWDHPFPRCEGRSQNNVLRHNGTIFSPGYPEEYPNSADCTWLISVAPSFGVKLNFTLLQVHGPHDFITVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTY3</accession>
    <name>A0A3B5KTY3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTY3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003578"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003617.1">
        <property type="protein sequence ID" value="ENSXCOP00000003578.1"/>
        <property type="gene ID" value="ENSXCOG00000002822.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156922"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052440">
        <property type="entry name" value="Trans_Reg/Chrom_Remod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14955">
        <property type="entry name" value="RETINOIC ACID INDUCED 1/TRANSCRIPTION FACTOR 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14955:SF8">
        <property type="entry name" value="SI:CH211-165G14.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13771">
        <property type="entry name" value="zf-HC5HC2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="125"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="231" mass="25123" checksum="DF9790F502B8BC25" modified="2018-12-05" version="1">MSLILGHLRFVSAVVPTTSCLYLGRLSTHGQHQRALVCCLCGRSANSIDLGDLHGPYYPEGYRPNIKAPANVSGLKEDEEDSSYSDSSSSTTRARRWAAPLKQKGLLGSRKWSSGRISSPEAKRARSEGGLADAEDWYSPPVLPVEPCEYWLHEDCGIWSAGVFLVRGKVYGLEEAVKVAQETMCTACHNPGATLGCFFKGCPNKYHYRCALESGEGSSFAFCVAVPLHST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KWN0</accession>
    <name>A0A3B5KWN0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Thioredoxin-interacting protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homodimer; disulfide-linked. Interacts with TXN/thioredoxin through its redox-active site. Interacts with transcriptional repressors ZBTB16, ZBTB32 and HDAC1. Interacts with DDIT4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KWN0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002937"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002974.1">
        <property type="protein sequence ID" value="ENSXCOP00000002937.1"/>
        <property type="gene ID" value="ENSXCOG00000002339.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158522"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF14">
        <property type="entry name" value="THIOREDOXIN-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="14">
        <location>
            <begin position="177"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="412" mass="45865" checksum="7219535AFCF9C4AC" modified="2018-12-05" version="1">MVAMTKKVKTFQITLSDSNKSFYCGGDKVCGRIEVEVSEVTRVSAMKILALGCAKVEYAKGKQRCRQEAEYLRYEEVLQLSEQPTDSDGSVVLRPGNKYEYKFGFDLPQQGQLVSSYKGKFGYVHYYVKALMERPQQPILECKKGFEVEEPLDVNTPDLLSPTGGTKEKKVTCMFIPDGQVSLNAKIDRRGFCEGEDICINAKFENTCSRIVVPKAAIIAKHTYQANGRTKIFRQKLSSVRGNHIISGMCDAWQGKTIRVPKIKPSMLGCNIIRVEYALMIYVHIPGSEKLILELPLVIGTAGLGSRSNSVSSQEGSVSNASQSWVSLRMPSEPPSYCDITKDCRLDQPLTPLLDDVDCDDSPIFMNAPSFQYPSPPTYTEVGISRQVGLLSDIIRHRCLIFTAGFLVLHII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KY26</accession>
    <name>A0A3B5KY26_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Basic leucine zipper transcription factor, ATF-like 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KY26"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000454"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000462.1">
        <property type="protein sequence ID" value="ENSXCOP00000000454.1"/>
        <property type="gene ID" value="ENSXCOG00000000399.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161120"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000837">
        <property type="entry name" value="AP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23351:SF13">
        <property type="entry name" value="BASIC LEUCINE ZIPPER TRANSCRIPTIONAL FACTOR ATF-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23351">
        <property type="entry name" value="FOS TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00042">
        <property type="entry name" value="LEUZIPPRFOS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="2">
        <location>
            <begin position="11"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="107" mass="12716" checksum="590B867E3A3D8D57" modified="2018-12-05" version="1">LQVWFLGLKDEGRRMKRKEKNRVAAQKSRKRQTQRADQLHQACELLEQRNRKLKREVDSLSEEQNFLSEALRAHEPFCPIMHFPLTLYFGPSDVIFIDNLISYSVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZR2</accession>
    <name>A0A3B5KZR2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(+) kinase</fullName>
            <ecNumber evidence="2">2.7.1.23</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZR2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003540"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003579.1">
        <property type="protein sequence ID" value="ENSXCOP00000003540.1"/>
        <property type="gene ID" value="ENSXCOG00000002783.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013792"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000003">
        <property type="entry name" value="NAD kinase b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF30">
        <property type="entry name" value="NAD(+) KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20143">
        <property type="entry name" value="NAD_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="435" mass="48689" checksum="B04E1E6D46AA6654" modified="2018-12-05" version="1">MGSSHAHQSGYTGHYCFVTIRAQWLNSVDLSVHLQPGNQRWTVDQSWMFVPLSSRRTQWLHGPSPVTTFGPKACMLQNPHAVMHIQDPASQRLTWNKPPKSVLVIKKIRDASLLQPFKELCTFLTEVKDLMVYVEKKVLDDPAISGDEVFAAITKKFCTFREDLDDISNRVDFIICLGGDGTLLYASSLFQESVPPVMAFHLGSLGFLTPFKFDTYQSQVTQIIEGNAAIVLRSRLQVRVLKENWEKKDTVDEKGIILTNKDLETSRKAMQYQVLNEVVVDRGPSSYLSNVDLFLDGHLITTVQGDGLIVSTPTGSTAYAVAAGASMIHPNVPAIMITPICPHSLSFRPIVVPAGVELQIKLSPDARNTAWVSFDGRKRQEICHGDSITITTSCFPVPSICFRDPVNDWFESLATCLHWNVRKKQNYLSSEDEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L0X8</accession>
    <name>A0A3B5L0X8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L0X8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004452"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004505.1">
        <property type="protein sequence ID" value="ENSXCOP00000004452.1"/>
        <property type="gene ID" value="ENSXCOG00000003517.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154649"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
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    </dbReference>
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        <property type="entry name" value="ZnF_C3H1"/>
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    </dbReference>
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        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="646"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="14">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
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        <location>
            <begin position="285"/>
            <end position="370"/>
        </location>
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        <location>
            <begin position="395"/>
            <end position="608"/>
        </location>
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        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="379"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="608"/>
            <end position="629"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
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        <source>
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        <source>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
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            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
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    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="667" mass="76279" checksum="C2E1DFA5B570D9F8" modified="2018-12-05" version="1">MAEILKLICANQGAVDADELMLNLFPGQSTTELISNHSKFALCSSKGKQRVVARTSLRLCRKKDCPGTCEGLHLCRNFLFSGSCHFLQRELQYDLIKPMTSLRNLFGRCASGVDCERLHICEKSLTQSCNCFRAHDFTAFQPKKILHDKMVPDDMLRSLRSVYSNREALRYYNRGEAGRGHRAEGAAETEVTTSRSYEAHKTEICMYFIKGYCKHDDKCFKAHDKMPYRWQVQEGGQWNALPDNETIEKEYCDPSNSYSSGSPPVHFDTMTRGENKVRRLTTVNSLVEPTFIHTTEWLWYWQDEFGKWNMYGSDSARGKSSDIRSETLEQKFLDNESEVEFSAGSQSYSLSVQDMIQTNRHYGTKRLVSRRPRFVSAADVRTKKVRKPAENSSPMPDHWDKAHIPPTGFSRVAVPRTSDEFQKIETLFRTTLRGFDIMKLERIQNKALWEVFQWQKTQMKNNNSGREVEEKQLFHGTDSKHIGAICLNNFDWRICGVNGTAYGKGSYFARDAKYSHSYTGDSDVKTMFVSRVLVGSYTRGDSSYVRPPSKDGGDINFYDSCVNDIANPSIFVVFEKHQIYPEYLLTYKTTHPLVDIISSTSAQQLKPVIAPTPRPSSKKGISVLRTPPPPPSKKCFIKSLDNLQHLFLFQTCFAYFLVVKFLLALLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L172</accession>
    <name>A0A3B5L172_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hippocalcin-like protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the recoverin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L172"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004547"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004603.1">
        <property type="protein sequence ID" value="ENSXCOP00000004547.1"/>
        <property type="gene ID" value="ENSXCOG00000003592.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165764"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Visinin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
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        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF84">
        <property type="entry name" value="HIPPOCALCIN-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0519">Myristate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
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    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
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            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022331"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="213" mass="24539" checksum="4BE8C5D23ABCF742" modified="2018-12-05" version="1">MGKHNSKLAPEVLDDLTKSTEFNEAELKQWYKGFLKDCPSGILNLEEFQQLYVKFFPYGDASKFAQHAFRTFDKNGDGTIDFREFICALSITSRGSFEQKLNWAFNMYDLDGDGKITRMEMLEIIEAIYKMVGTVIMMRMNEDGLTPQQRVDKIFSKMDKDHNDEITLEEFKELCVCVCMWCVCVCACVFKGLLFECSVFCICIICVTRDASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L186</accession>
    <name>A0A3B5L186_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nucleoside diphosphate kinase B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Major role in the synthesis of nucleoside triphosphates other than ATP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 12">Belongs to the NDK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L186"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001564"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001589.1">
        <property type="protein sequence ID" value="ENSXCOP00000001564.1"/>
        <property type="gene ID" value="ENSXCOG00000001267.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177394"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902176">
        <property type="term" value="P:negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46161">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46161:SF1">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE HOMOLOG 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="13">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="99" mass="10786" checksum="350234DF9374676D" modified="2018-12-05" version="1">MGPSNPEKAKEISPQSLRARFAADILHNSVHGSSNEQHAEEKIRFVFGDISADAAPADDSETSRTTSGILIDFNNLLTTTELKSLLDSSVRMRQPADQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1P1</accession>
    <name>A0A3B5L1P1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA repair endonuclease XPF</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1P1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004255"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004302.1">
        <property type="protein sequence ID" value="ENSXCOP00000004255.1"/>
        <property type="gene ID" value="ENSXCOG00000003345.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004394"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20078">
        <property type="entry name" value="XPF_nuclease_XPF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000002">
        <property type="entry name" value="DNA repair endonuclease XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006167">
        <property type="entry name" value="XPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047520">
        <property type="entry name" value="XPF_nuclease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00596">
        <property type="entry name" value="rad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150:SF0">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="12">
        <location>
            <begin position="660"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="435"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="621"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="451"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="476"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="489"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="832" mass="94568" checksum="94768178D35E3DA7" modified="2018-12-05" version="1">MKFCCSRVLSEELQLTGSEHTRICLQISGNLFILKSKLPNSFIYFYRRFRPELLQRRLGPGFHLDQTPGRSRVGPDHPAQLGFMRRCFHRIRVKSQDWSSFMTISGKTDEIITLILHECIHVFTGILVYRAHKIMESCQEAFILRLFRQKNKTGFIKAFTDKATSFCSGFCQVERVMRNLFVKKLYLWPRFQASVNAALDRHKPEVVELHVSLTPAMRAIQSSILDIMSACLKELKRYNPSLEAEDLSLENALGNAFEKTIRHYLDPLWHQLGAKTKALVQDLKVLRVLLLYLTQYDCVTFLNLLESLRSSQKTFGSNSGWLFLDCSTAMFVNARSRVYRCPDGGKKPKLTAENHKGQRVPEFKRELVLEKSPKWEVLTEVLDEIKKENQNSQQEPGRVLICASDDRTCAQLQQFIKHGSDWLLNRLYARTVGKRDSAAAAVTSDPDSLKKGKGRLKKGGKGKEPAQKRSTKTGKSRASLTLTQMMSKEEEEERGGDDDEDHLMEEEEEQLNLNLSSDSYYGVLKEPLTVIHPLRGCSDPHSLTRVLQEVQPAFVVLYDAELSFVRQLEVHRAGRPGKPLRVYFLIYGGSTEEQRYLTALSHEKKAFELLIREKATMVIPEEREGRENTNLDLARSLEPANSATNSRRAGGQEPGAAPSRVIVDMREFRSELPALLHRRGLDIEPVTLQVGDYILTPDICVERKSVSDLIGSLQSGRLYTQCLAMTRCYRKPLLLIEFDPAKPFSLTARSDFRSEISSADVSSKLTLLTLHFPRLRILWCPSPHATADLFQELKLVSVVIMMSSSAAEKQTIILQIFQVICGSASCFLFSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1T4</accession>
    <name>A0A3B5L1T4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">chymotrypsin</fullName>
            <ecNumber evidence="9">3.4.21.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.1" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1T4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004762"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004820.1">
        <property type="protein sequence ID" value="ENSXCOP00000004762.1"/>
        <property type="gene ID" value="ENSXCOG00000001740.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154494"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000176">
        <property type="entry name" value="Chymotrypsinogen A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000181">
        <property type="entry name" value="Chymotrypsinogen A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24250:SF66">
        <property type="entry name" value="CHYMOTRYPSIN-LIKE PROTEASE CTRL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24250">
        <property type="entry name" value="CHYMOTRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0222">Digestion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017230168" description="chymotrypsin" evidence="11">
        <location>
            <begin position="19"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="12">
        <location>
            <begin position="34"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="263" mass="28490" checksum="A649F90EF9C0792F" modified="2018-12-05" version="1">TVMFLLISCFALVASTLGCGVPTIKPQVSGYNKIVNGENAVSGSWPWQVSLQDSTGFHFCGGSLINEYWVVTAAHCRVSPSNHRVILGEHDRQYNSEPIQVKTISRAITHSYFNSNTLNYDITLLRLSSPAQFNSRVSPVCLVSSSSSIPSGTKCVTTGWGRTGQTSSPRYLQQTSLPLLSPAQCRNYWGTSRITDVMICAGASGVSSCQGDSGGPLVCESSGVWYLVGIVSWGTTDCNVYTPAVYARVSYLRSWIDQTLAYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2K8</accession>
    <name>A0A3B5L2K8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MSL complex subunit 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the msl-1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2K8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004590"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004646.1">
        <property type="protein sequence ID" value="ENSXCOP00000004590.1"/>
        <property type="gene ID" value="ENSXCOG00000003624.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018292"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072487">
        <property type="term" value="C:MSL complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000047">
        <property type="entry name" value="male-specific lethal 1 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026711">
        <property type="entry name" value="Msl-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031840">
        <property type="entry name" value="MSL1_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029332">
        <property type="entry name" value="PEHE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21656:SF2">
        <property type="entry name" value="MALE-SPECIFIC LETHAL 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21656">
        <property type="entry name" value="MALE-SPECIFIC LETHAL-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16801">
        <property type="entry name" value="MSL1_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15275">
        <property type="entry name" value="PEHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01300">
        <property type="entry name" value="PEHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52052">
        <property type="entry name" value="PEHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PEHE" evidence="8">
        <location>
            <begin position="324"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="152"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="225"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="240"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS52052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="465" mass="53083" checksum="124EF6BE6B7791AC" modified="2018-12-05" version="1">MTSKVALAATAPETVQGDKPLGITSPVRPMGGEGTPVKGKPLSVDNMDNPQMAVNNNPKDAGAEDSTKGVVPGVLGTASIELSSEGKWRNIRKTPASPHAQANCLRQILLLQLDLIEQQQQQLQSKDKEIDDLKADKETLLARIERMERRLQLTRKDMPRDKRLFQPLEPWTPDKEDMWDLDIEESPQPNAATPLPFSRGGKGQKRKTCFGEAKVQKSRGKSAKLSPQKSETEPGSPNQRELRSKETPEKSVPARSGAERDALLPCKEEPELSCQMEDLPFMSTTEMYLCCWNQPPLSPLRETSPKKEEEVASEWTPHVVHDMLIVFPSWRENHIEPLEEDACNIPEPLDDGTFLKRHAKLELDEKRRKRWDIQRIREQRVFQRLQQRMNRKKTVQETEPELSSFYPETEDVEAIVITPFLPVVAFGRPLPKLSQQNFELPWLDERSRCRIEVPKKHTPHRTCRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2V8</accession>
    <name>A0A3B5L2V8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Radical S-adenosyl methionine domain containing 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2V8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005137"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005198.1">
        <property type="protein sequence ID" value="ENSXCOP00000005137.1"/>
        <property type="gene ID" value="ENSXCOG00000004023.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013670"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050778">
        <property type="term" value="P:positive regulation of immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026372">
        <property type="entry name" value="RSAD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051196">
        <property type="entry name" value="RSAD2/Viperin_antiviral"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04278">
        <property type="entry name" value="viperin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038283">
        <property type="entry name" value="viperin_w_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21339">
        <property type="entry name" value="RADICAL S-ADENOSYL METHIONINE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21339:SF0">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT NUCLEOTIDE DEHYDRATASE RSAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="8">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="247" mass="28772" checksum="42F7095ED02B14AF" modified="2018-12-05" version="1">MRMEKINFSGGEPFLHERGDFLGKMVQYCKTELQLPSVSIVSNGSMIKEKWFQKYGDFLDILAISCDSFDEETNQMIGRSQGKKSHIENLFKIRSWCQEYKVAFKINSVINTFNVHEDMTEQINQLNPVRWKVFQCLLIDGENAGEAALREAETFVITDQQFQDYLDRHSAVSCLVPESNEKMRNSYLILDEYMRFLDCREGRKDPSRSILDVGVKDAISFSGFDEKMFLKRGGKYVWSKADMKLEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2Z7</accession>
    <name>A0A3B5L2Z7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome activator complex subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Proteasome activator 28 subunit alpha</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterodimer of PSME1 and PSME2, which forms a hexameric ring. PSME1 can form homoheptamers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PA28 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2Z7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006688"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006770.1">
        <property type="protein sequence ID" value="ENSXCOP00000006688.1"/>
        <property type="gene ID" value="ENSXCOG00000005176.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183098"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:proteasome activator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000045">
        <property type="term" value="P:regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061136">
        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.180:FF:000002">
        <property type="entry name" value="Proteasome activator complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.180">
        <property type="entry name" value="Proteasome activator pa28, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003186">
        <property type="entry name" value="PA28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036997">
        <property type="entry name" value="PA28_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009077">
        <property type="entry name" value="Proteasome_activ_PA28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003185">
        <property type="entry name" value="Proteasome_activ_PA28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036252">
        <property type="entry name" value="Proteasome_activ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10660:SF5">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10660">
        <property type="entry name" value="PROTEASOME REGULATOR PA28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02252">
        <property type="entry name" value="PA28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02251">
        <property type="entry name" value="PA28_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47216">
        <property type="entry name" value="Proteasome activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome activator PA28 N-terminal" evidence="8">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator PA28 C-terminal" evidence="9">
        <location>
            <begin position="115"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="69"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="259" mass="29655" checksum="3A9F9E739E4A4D53" modified="2018-12-05" version="1">MTLNLRNRLSGTTSVDKAQFLPKQLKVLRSETKNIHGVFYEKTSPLLGVLLLWQTSFSCDDLASLKSPLDIPIPDPAKEEAKRKKKEEKEAKEGKKDKDSDKEEEDSGPPCGPICSNERVESLLNEVKPEIQTLKEKLNTVSMWVQLQIPRIEDGNNFGVAVQEKVFELLTNTRTKIEAFQTQISKYYSERGDAVAKASKQPHVGDYRQLVHELDQYQYRELRLVVLDIRNTYAVLFDIINKNYDKIKKPRGDGKALIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L392</accession>
    <name>A0A3B5L392_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">QKI, KH domain containing, RNA binding 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L392"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005277"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005338.1">
        <property type="protein sequence ID" value="ENSXCOP00000005277.1"/>
        <property type="gene ID" value="ENSXCOG00000004131.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:skeletal myofibril assembly"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="KH-I_Hqk"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="protein quaking isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032367">
        <property type="entry name" value="Quaking_NLS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF152">
        <property type="entry name" value="PROTEIN QUAKING-B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
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    </dbReference>
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        <property type="entry name" value="Quaking_NLS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="6">
        <location>
            <begin position="75"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="326" mass="36622" checksum="9129BFA438199E91" modified="2018-12-05" version="1">WFLMLFAMVSHDRTQLDSLIQVMQLKRLNTVQSKCISVPDEIGRVRKDMYNDTLNGGMFNGRDMEELPEAIGPVAQLQEKLYVPVKEYPDFNFVGRILGPRGLTAKQLEAETGCKIMVRGKGSMRDKKKEEMNRGKPNWEHLSEDLHVLITVEDTHNRAKIKLQRAINEVKKLLVPAAEGEDNLKKMQLMELAILNGTYRDANVKTPTAFPLTTPQAPRIITGPTPVLPPTLRNPAPVTTPTIMPLIRQIQSSALVPGANPHPALVQQGPESGIIYTPYEYPYTLTPSILEYPIDSTGVLGAMTTKVRRHDKRIHPYQRVVTTDRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L3T5</accession>
    <name>A0A3B5L3T5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSXCOG00000003956.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:monoacylglycerol catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:phosphatidylserine catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF69">
        <property type="entry name" value="PROTEIN ABHD12B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="283" mass="31461" checksum="762D65F0AFE5CFE8" modified="2018-12-05" version="1">RRRMKKVLSFLLTAYVSVPVLLYLFPWILSYAIFSHLLRLPYSVDLRRPQDVLNHTCNFYLSPEEGVSVGVWHTLPASQWEAAAGRNLDWYQEALGDGRPVIIYLHGNVGTRWTVALVVVGFGDSSGEPSEAGLTNDALYLHQWAKARSRGIICLWGHSLGSGRVFGTSGSAVDALILEAPYTRIGEIENILGCLFFFFALIISDICCRFCSLQTLSSPLLILHAEDDKVVPFHMGQKLYQISLRAQRESETGDRVQMISYGANLGLSHSRICSARDLAGVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L441</accession>
    <name>A0A3B5L441_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L441"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007088"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007176.1">
        <property type="protein sequence ID" value="ENSXCOP00000007088.1"/>
        <property type="gene ID" value="ENSXCOG00000005453.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158601"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09913">
        <property type="entry name" value="EHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000147">
        <property type="entry name" value="EH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000038">
        <property type="entry name" value="EH domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF5600"/>
    </dbReference>
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        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
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    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF57">
        <property type="entry name" value="EH DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18150">
        <property type="entry name" value="DUF5600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="15">
        <location>
            <begin position="41"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="13">
        <location>
            <begin position="430"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="462"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="509"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="531" mass="60465" checksum="6370B6546252D254" modified="2018-12-05" version="1">RKATLKEKRHYMEVDGNLYLYSAGTWCAFPVHHANIMPAGCVYVFLNIHLLSTKIQSFDSKQYLLEQDFPGMRIGPEPTTDGFIAVMHGETEGVVPGNALVVDPKKPFRKLNAFGNSFLNRFICSQMPNQVLQSISIIDTPGILSGEKQRISRGYDFAEVLRWFGERVDRIILLFDAHKLDISDEFSEAIKAFKGQDDKIRVVLNKADQVDTQQLMRVYGALMWSLGKVINTPEVVRVYLGSFWAKPLQNTENRRLFEAESQDLFRDIQSLPRNAALRKLNDLIKRARLAKVHAYIISYLKKEMPSLFGREKKKEELIMRLPEIYVTLQREHHISPGDFPNVTKMQDMLQHYDFSKFPSLKIKLIESVDKMLATKIAVLMAMIREEESKLPPAMVSGGAFEGSEDGPFGRGYGEGICAGADADDWIVSRDKHRYDEIFYTLMPVNGKITGVNAKKEMMNSRLPNTVLGKIWKLADCNQDGMLDDEEFALAQHLIQIKLEGYELPNELPNHLVPPAHRKNPTADALYNHTED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L862</accession>
    <name>A0A3B5L862_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Selenoprotein K</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the selenoprotein K family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L862"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003979"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004023.1">
        <property type="protein sequence ID" value="ENSXCOP00000003979.1"/>
        <property type="gene ID" value="ENSXCOG00000003129.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016119"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032469">
        <property type="term" value="P:endoplasmic reticulum calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Se_SelK/SelG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16875">
        <property type="entry name" value="SELENOPROTEIN K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16875:SF0">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10961">
        <property type="entry name" value="SelK_SelG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="51"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="92" mass="9829" checksum="C325BA118F51AC8F" modified="2018-12-05" version="1">LFFVSPSGQVLDSRSQSPWSLSFLTDMFWGAVEFISLFFRTILHPDLSKKGNVASSRFSDGRGPPGPPGGRRRMGRITHGAGPNPPPMGGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9V3</accession>
    <name>A0A3B5L9V3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Melatonin receptor 1A a</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">High affinity receptor for melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibits adenylate cyclase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9V3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007612"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007708.1">
        <property type="protein sequence ID" value="ENSXCOP00000007612.1"/>
        <property type="gene ID" value="ENSXCOG00000005740.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160321"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008502">
        <property type="term" value="F:melatonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000056">
        <property type="entry name" value="Melatonin receptor type 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002278">
        <property type="entry name" value="Mel_1A/1B_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000025">
        <property type="entry name" value="Melatonin_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228">
        <property type="entry name" value="B2 BRADYKININ RECEPTOR/ANGIOTENSIN II RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24228:SF53">
        <property type="entry name" value="MELATONIN RECEPTOR TYPE 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01149">
        <property type="entry name" value="MELATONIN1AR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00857">
        <property type="entry name" value="MELATONINR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="185"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="236"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="44"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="377" mass="42719" checksum="7019A05A5355290B" modified="2018-12-05" version="1">MVINGSHLNSSAQDPSDAALSRPPWVTTTLGCFLIFTIVVDILGNLLVIFSVFRNKKLRNAGNIFVVSLAVADLVVAIYPYPLVLTSIFHNGWNLGNVHCQISGFLMGVSVIGSIFNITGIAINRYCYICHSLKYDKLYSDKNSVCYVILIWALTVVAIVPNLFVGSLHYDRRVYSCTFKQSASSAYTIAVVFFHFILPIMIVTYCYLRIWILVIQVRRRVKPDNRPKLTPNDVRNFVTMFVVFVLFAVCWAPLNFIGLAVAIKPEVVVPLIPEWLFVASYFMAYFNSCLNAIVYGVLNQNFRREYKRIVVSVCTARIFFQDSSNDAAERLKSKPSPLMTNNNQVKRELSLHPVKSLYCMHGYKNVFICKIKSNIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LA51</accession>
    <name>A0A3B5LA51_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus membrane</location>
            <topology evidence="1 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="RefSeq" id="XP_027884232.1">
        <property type="nucleotide sequence ID" value="XM_028028431.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027884233.1">
        <property type="nucleotide sequence ID" value="XM_028028432.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027884235.1">
        <property type="nucleotide sequence ID" value="XM_028028434.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027884236.1">
        <property type="nucleotide sequence ID" value="XM_028028435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LA51"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006616"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006697.1">
        <property type="protein sequence ID" value="ENSXCOP00000006616.1"/>
        <property type="gene ID" value="ENSXCOG00000005122.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114151306"/>
    <dbReference type="KEGG" id="xco:114151306"/>
    <dbReference type="CTD" id="8707"/>
    <dbReference type="GeneTree" id="ENSGT00940000155117"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008499">
        <property type="term" value="F:N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000001">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045821">
        <property type="entry name" value="B3GT2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF19">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19341">
        <property type="entry name" value="B3GALT2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-1,3-galactosyltransferase 2 N-terminal" evidence="15">
        <location>
            <begin position="76"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF19341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="441" mass="50340" checksum="4B702EFEFCFD7836" modified="2018-12-05" version="1">MQWRRRHLCSIKMTWTIKRSLFRTHVAGLLSLALLFTVFLFFSHQEWLPGRSGPRDYPLAYTIRGLRAPKGEANQSSSLRSLWKETGYGAPKPLLNLSSQQAEGTAAEPGGGGAGGGSRTGIMGLGDSMSSNNSLQKEMDVGEKLSAQPYRYILNEPYKCRDSTPFLILLIAAEPRQADARNAIRQTWGNESVAGGLGFVRLFLLGTGKSSDAFLQSSIEEESRIYHDIIQQDYQDTYYNLTTKTLMGMNWVATYCPRVSYVMKTDSDMFVNTEYLIQKLLKPELPPKQKYFTGYLMRGYAPNRNKDSKWYMPPELYPSERYPIFCSGTGYVFSGDMAELIYQASLSIRRLHLEDVYVGICLAKLRIDPSPPPNEFLFNHWRVSYSSCKYSHLITSHQFQPNELIKYWNHLQTNKHNACVNTAKEKNGRHRHRKLHGERLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LA58</accession>
    <name>A0A3B5LA58_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">IKAROS family zinc finger 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the Ikaros C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LA58"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009073"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009184.1">
        <property type="protein sequence ID" value="ENSXCOP00000009073.1"/>
        <property type="gene ID" value="ENSXCOG00000006917.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158308"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000073">
        <property type="entry name" value="IKAROS family zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000080">
        <property type="entry name" value="IKAROS family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002707">
        <property type="entry name" value="IKAROS family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000168">
        <property type="entry name" value="zinc finger protein Eos isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050589">
        <property type="entry name" value="Ikaros_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404:SF28">
        <property type="entry name" value="ZINC FINGER PROTEIN EOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="212"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="304"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="314"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="336"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="441" mass="48590" checksum="26DFCD8874D5AA18" modified="2018-12-05" version="1">MNADECNGRSYSQGESSAEQDFYAGLRATSVRSPNSQQSSPHRSLSDSIKVELCSDEESSGARQSENKEAARSDGGRDDRVGPADEGGPAFAGTGGDRGNIYSEMATPNSSSPGPIRLPNGKLQCEVCGMVCIGPNVLMVHKRSHTGERPFQCNQCGASFTQKGNLLRHIKLHSGEKPFKCPVCNYACRRRDALAGHLRTHSVPSPTVGKPFKCNYCSRSYKQQSTLEEHLERCHSYMKTLDSQMPPGNTSYISVNMESITKPVLQPSNEKRPFVDRLTVSITKRKRSTPQKFLAMSLGLHGREAGEARDDQPSAHSHTTSPNGCPDSTDTESTAEEQSTRATAPTSTSNNHHLHYLTPALPRGSPLSSRNNFQVLDRGGRPVRSFYCRHCCILFLDHVMYTIHMGCHGFRQPFECNICGHPSQDRYEFSSHISRGEHQVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LB36</accession>
    <name>A0A3B5LB36_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">G protein-coupled receptor 61</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LB36"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008042"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008140.1">
        <property type="protein sequence ID" value="ENSXCOP00000008042.1"/>
        <property type="gene ID" value="ENSXCOG00000006178.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182998"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15220">
        <property type="entry name" value="7tmA_GPR61_GPR62-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752:SF5">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="63"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="464" mass="51440" checksum="F385BD9D5EB6D935" modified="2018-12-05" version="1">MEHTIRPRPSWNNASAASTFQNSFRPNVSNITRQIGTTDNGIDLNQILGLCAMLVMDVLAVVGNLAVMIVITKTPQLRKFAFVFHLCLVDLLAALLVMPLGMLSDRILEDEALCRSYLCLSVCLVSAAILTICAINVERYYYIIYPMRHEVKMTVGVVVAVLVGIWIKALVMSMLPLLRYLFQDSPTVGTPEVLLPSKRHCSLHWTGGRTTRLVFMVFFTVIYFLCPMLIILVVYCNMFKVARVASMHHGPNPTWVDTSRQRSDSVSSHSTMAASLGGTGARTTPQRTFSGGKAAAVLVAVSGQFFCCWLPYFSFHLYSAVISTAPASLTQLEDLVTWIGYFCFTSNPFFYGCLNRQIREELGRHLACIFKRAGSVEGEQLPSREASIEENFLQFLQGTGCNLEPCNSHSRASPEEAGTEGLQESAVEQNTPADFPIPGQILEETAEFIQQQQLNNELCVSENC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCT5</accession>
    <name>A0A3B5LCT5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FERM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCT5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005684"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005750.1">
        <property type="protein sequence ID" value="ENSXCOP00000005684.1"/>
        <property type="gene ID" value="ENSXCOG00000004421.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230354"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13194">
        <property type="entry name" value="FERM_C_ERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000013">
        <property type="entry name" value="radixin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011174">
        <property type="entry name" value="ERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011259">
        <property type="entry name" value="ERM_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041789">
        <property type="entry name" value="ERM_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046810">
        <property type="entry name" value="ERM_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008954">
        <property type="entry name" value="Moesin_tail_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23281">
        <property type="entry name" value="MERLIN/MOESIN/EZRIN/RADIXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00769">
        <property type="entry name" value="ERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20492">
        <property type="entry name" value="ERM_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BAND41"/>
    </dbReference>
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        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48678">
        <property type="entry name" value="Moesin tail domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="5">
        <location>
            <begin position="5"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="282"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="406"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="406"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="438"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="469"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="540" mass="63666" checksum="FF6A51F37B356237" modified="2018-12-05" version="1">FPPQVNVRVTTMDAELEFSFNGNTKGRQLLEQVARTIGLREIWYFGLQFVDNRGLASWLVEDKKVTEQNVKNETPLQFRFRVQYYPEDVDEELIQDVTRKLFFLQVKEHILSDEIYCPPETAVLLASYAVQAKYGEYDKSVHRREYLSSERLLPKREDAIIEYLKIAQDLEMYGVSYFEIRNKKGTELWLGVDALGINIYPKDDRLTPKIGFPWNEIGNVSFDNKKFVIKPMDKKSPHIMFHGTQPRIHKGIMQLAKGNHRLYARRRVADNIEVQQMKAQAKQDRLQKKMERDQLESEKKKRENIEREKQQMEREKQELMMKLYEFEETTKRAERDLQEQLDRAMRLEEERRRVEQEAARLEAERMEAIIAKEELARQAEEQMKSQEQLAAELAEYTAKITELEEAKRAKEEEAETWHMKVSTLTGGQGKTARRSGGKKSESDHEHSEENSTYSTELHSQDISEDLLEEERLTEAEKNERLRKQLMDLSSDLEQSRHDSKNTQNDLLHAENVRAGRDKYKTLRQIRLGNTKQRVDEFEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDC7</accession>
    <name>A0A3B5LDC7_9TELE</name>
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        <recommendedName>
            <fullName evidence="4">Troponin I type 2a (skeletal, fast), tandem duplicate 2</fullName>
        </recommendedName>
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    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="32473.ENSXCOP00000008555"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008661.1">
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        <property type="gene ID" value="ENSXCOG00000006547.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="entry name" value="Troponin coil-coiled subunits"/>
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    </dbReference>
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        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="12696" checksum="DD2411F47BDD90FE" modified="2018-12-05" version="1">VSACRKRLSARRKHTLKSCMLVVANNLLEAESQVKAKERETFLAEKCPPLDVPYSREELVELCKKLHQQINISEEERYCTEFKLNMVLNEVRTPQSAGSVWRETGRDVTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEL9</accession>
    <name>A0A3B5LEL9_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSXCOT00000009398.1">
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        <property type="gene ID" value="ENSXCOG00000007074.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017329999" evidence="1">
        <location>
            <begin position="19"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="62" mass="7266" checksum="601261017D1486BB" modified="2018-12-05" version="1">MKGLSLVLLVLLLMFATAEEDPEMQYWTCGYRGLCRRFCYAQEYIVGHHGCPRRYRCCAVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEM3</accession>
    <name>A0A3B5LEM3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arylamine N-acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the arylamine N-acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.5" evidence="2"/>
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        <property type="nucleotide sequence ID" value="XM_028005475.1"/>
    </dbReference>
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    <dbReference type="STRING" id="32473.ENSXCOP00000009020"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009129.1">
        <property type="protein sequence ID" value="ENSXCOP00000009020.1"/>
        <property type="gene ID" value="ENSXCOG00000006879.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114137003"/>
    <dbReference type="KEGG" id="xco:114137003"/>
    <dbReference type="GeneTree" id="ENSGT00390000012054"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004060">
        <property type="term" value="F:arylamine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2140.20:FF:000001">
        <property type="entry name" value="Arylamine N-acetyltransferase 1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Arylamine_N-AcTrfase"/>
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        <property type="entry name" value="Arylamine_NAT_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786:SF3">
        <property type="entry name" value="ARYLAMINE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786">
        <property type="entry name" value="N-HYDROXYARYLAMINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00797">
        <property type="entry name" value="Acetyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01543">
        <property type="entry name" value="ANATRNSFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="283" mass="31967" checksum="F35CD8A355454821" modified="2018-12-05" version="1">MEVDRYLSRIGFEGPSGVPSLELLRSVHTCHLLSVPFEDLTVHSGGQVKLDLPVLYDKVVNQRRGGFCFENNSLFYWLLTQLGFQVTVLSGQVKNSITSCYGPPFDHLIIMVNLEGKRWLCDVGFGAAGFSAPLSLDTCGPQEQGHRVYRLRRIDDVHFLEWQKEENRGEDGDWTEIYKFTLEPRRLEDFTQMCQYHQSSPSSIFFCKSLCTVLRPGGRVSYIGRRLITTRFPSEATGGGLETTTRELEDEEIPAVLAENFGIVLQSPLIPKDDTITPPPVVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LF24</accession>
    <name>A0A3B5LF24_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DEP domain containing 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LF24"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010768"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010893.1">
        <property type="protein sequence ID" value="ENSXCOP00000010768.1"/>
        <property type="gene ID" value="ENSXCOG00000008126.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182976"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000182">
        <property type="entry name" value="DEP domain-containing protein 1B isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16206">
        <property type="entry name" value="DEP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16206:SF12">
        <property type="entry name" value="DEP DOMAIN-CONTAINING PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEP" evidence="3">
        <location>
            <begin position="24"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="742" mass="84051" checksum="335D3530FCB7C46E" modified="2018-12-05" version="1">MDSHIITPGPYRATKLWNEVTRLFRAGMPVRKHRQNFRHHASCFTAAAAVDWLHQLLQSNSNFGPEVTRQQTLQLLKKFLKNHVIEDVKGRWGTEDLEDNHMLFRFPSASPLKPIPCPAPAPDSRSIKKRPSLRDREGFFRLRNFKKQEKDSMQQHQRRELTLEDEQEIWRDVTLTHLQRILGVSSLDEVLDQRHINPQNIIHNMTNVNKHGVVTLEDKSNDLPHWVLSAMKSLANWPKYDSDQPSYPGFERDVFKTVSDYFYSLPQPLLTYELYELFINVLVMCGYVAAPPTLQRGKRKSSELPSVAAPPAKSLFLSTECLLLSLLRQGSCDEVESPMRDVLSGTLQSRKESGGTNLKTVRLRTRSCSLETILDRSPPPPCGLQPFPSSDENLVFPPQEGHLNTTFTTKANSFVHENAAGVVTRRKRSAGSRVRPLDGQASARPRSASSCLDIVMETKEEEYGGMKWSSSHGSAPACSTSNLWALPAPAVRRCLSSLDVSKPPQPHRSASQLNLPVCVLQPQCERVAIEALQLCTLLLPPASRRKLQLLMRMASRISQNVDMPRLHPAIGTRTLMVHTFSGCVLSSAEDCDLDELLATRLVSFLMDHQQSVLSVPEYLRSAIRDHIHYLLKSDSSCFIDGADPVCVPMPMYAFCKQISGAEFEQQKAESSQKAMEELLEILLTDRNLTEKDRRKKLKQFQRQYPDIYSRRFPSCGQQSKTDSKPKIKPPLLNIKKTFSIRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LG25</accession>
    <name>A0A3B5LG25_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-processing factor 39</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">PRP39 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PRP39 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LG25"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009540"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009652.1">
        <property type="protein sequence ID" value="ENSXCOP00000009540.1"/>
        <property type="gene ID" value="ENSXCOG00000007266.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005033"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030627">
        <property type="term" value="F:pre-mRNA 5'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000063">
        <property type="entry name" value="Pre-mRNA processing factor 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000091">
        <property type="entry name" value="Pre-mRNA-processing factor 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059164">
        <property type="entry name" value="HAT_PRP39_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204">
        <property type="entry name" value="PRE-MRNA PROCESSING PROTEIN PRP39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204:SF21">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23241">
        <property type="entry name" value="HAT_PRP39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="666"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="83"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="684"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00080852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="745" mass="85625" checksum="CBF02D906A17CC58" modified="2018-12-05" version="1">MESNGNEFLPDLPAQAPDWSLEQVPPQPAASVAPEGSDSSQDDLAQEQQQSQQTNSSELGSVEKAVEQFQLANASLFQEEHPPPPPPPPPPPPTEDPDQPLQFATEPQPEAFMEDGMELEEPARESSQEIPVPTEPTPSSEFEKLFKICEDSPEDFNAWVSVLQYVEQENLLLPARKSFDVFFLRYPYCYGYWKKYADMEKKHGNLQVSEEVYRRGLQAIPLSVDLWLHYLTFIKENSDPNDPETEGRIRASYEHAVLAAGTDFRSDRLWESYISWETDQQKLANITTIYDRVLGIPTQLYSQHFQRFKDHVQGNHPKHFLSEEEFVQLRLELSKSSLSGMVGEDGESNVPQEELPPGTEDLADPAKRMTEIENMRHKVIEVRQEVFNHNEHEVSKRWSFEEGIKRPYFHVKALERTQLNNWKEYLDFEIENGTPERVVVLFERCLIACALYEEFWIKYAKYLEGFSTDGARHVYKKACSIHLPKKPAIHLLWAAFEEQQGNVEEARGILKALEETIPNLAMVRLRRVSLERRRGNLDEAEALLKEGMETANNDMESSFYAVKLARHLVKVQRSLSKAKKVLLDAIEKDQTSSRLYLNLLELEFSGDVMQNQAEILACFDRALRSPLSLDSRIIFSQRKVEFLEDFGSDINALVAAYEEQQKLQKESDSLKRTAENGYDSSQEPDAKRQRLDDPSAVAANAMTDMQANSSAYNYNWYQYGGWGQNSWGQYNQYAQYNQYYPPPPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGA3</accession>
    <name>A0A3B5LGA3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Myosin XVAb</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000006889"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006977.1">
        <property type="protein sequence ID" value="ENSXCOP00000006889.1"/>
        <property type="gene ID" value="ENSXCOG00000005326.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155335"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000201">
        <property type="entry name" value="Myosin XVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.530">
        <property type="entry name" value="MyTH4 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059004">
        <property type="entry name" value="MYO15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000857">
        <property type="entry name" value="MyTH4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038185">
        <property type="entry name" value="MyTH4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051567">
        <property type="entry name" value="Unconventional_Myosin_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692">
        <property type="entry name" value="MYOSIN VII, XV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22692:SF21">
        <property type="entry name" value="MYOSIN XVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26570">
        <property type="entry name" value="MYO15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00784">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00139">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51016">
        <property type="entry name" value="MYTH4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="8 11" id="KW-0518">Myosin</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Myosin motor" evidence="14">
        <location>
            <begin position="10"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="MyTH4" evidence="13">
        <location>
            <begin position="789"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="domain" description="MyTH4" evidence="13">
        <location>
            <begin position="1525"/>
            <end position="1662"/>
        </location>
    </feature>
    <feature type="domain" description="FERM" evidence="12">
        <location>
            <begin position="1667"/>
            <end position="1986"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="101"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1986" mass="226918" checksum="72EF5C86509D4F98" modified="2018-12-05" version="1">MPTTNHRVVRWAEGMEDMTELNETTVLMNLKKRFDHELTYTYIGNILVSVNPYKLLNIYGTDMVHQYEGHGLSDNPHLFAIANLSYTSMMDAKKDQCIVIGESGSGKTEATKLILRYLTAINYKRNVTQQILEATPLLESFGNAKTVRNDNSSRFGKYTQIYMEGVISGAITSQYLLEKSRIVFQAKSERNYHIFYEMLAGLPPHEKRSLYLQEAETYYYLNQGGNCTIEGKDDGEDFRRLLSAMDILCFTPEEQNGIYRLLSSVLHLGNVYFQVEGQEAAAVVSAQEIRVVAELLQVSPESLQKAVTYKMTDTGREQIYTPLTVDSALDADAVAKILYSLLFSWLTERINGRVYPRTEALSISVLDIYGFEELQVNSFEQLCINYANETLQFYFNRVVFQEEQLQEEYMREQIQWQPQLFSNNQACLDLIAAKPHGILRILDDQCGFPQATDHTFLQKCHYHHGNDPLYARPKMPLPEFTLKHYAGKVTYQVHKFLDKNFDLVRQDVLDLFIQSRNRMVSSLFLKHSESLTQQRSNLRRNSAARRHQTNTVSAKFQSSLQELLDKMKRCNPYFVRCIKPNRHKHRYKALLCLKDPLPADGENCVIMLHKLCPVKEGSYQVGIFLKEELYQLLEGKRDRVLDIAAMVLQRYTRMCFARQKFLKFRQGITRFEARCRGYLVKKFALRRKYLIRLRSAVLLIVNRQRYMRVFCVQDRINREVMNVTTLPIPAELAGLLQAASGEELHSDCLAVVQAPKVQVDPQLTLPLDINNYLLTHYIRIMFREPLFGMLTAPLENSLIRLDQELTQGSLDIFILILRFMGDPNLNGAQENMFGNYIIQRGLANPSLRDEILAQIANFVSDYGPDGYDCVCQNRLLQALQRLAVGPEYVRTYPPCLLEWTANRKKAHTVLHVHCFDMSFLCPLHSWTTGEEMAKNILQHGAVDGRQGWSVLLKEPAQWVELEGSDYVLDLMSDLELPADFPKHNSYFIISALEPARVRPNASLEPHRGMDRYLDSLFDPVLSDGAGQQAVLAQQQQAIVNQQAVIMQAQQMTMQAMAMVSSPVSSPPTTPITSPPMSPVPLHVLASPYAVIPPSPYANIPPSPYPPPAGEVVKYSSQTADHIVPSQDIQRPDEKFKKKQTPRDEALQILKPQMDNPPAPQSRELPVETKTIQTQLHQSTNQEHYTYTNVPWRLYLRKEPQVFYPKDSFNNPLLLDLIFKQIVNDTLSEACVRITRDERHKMKALFKHGVEQNMDPVEEHVKKTIVTAARETWEIYFSRLFPASGSVETGVQVLSVSHSGIKLLKTVKSSASAPDYFRVLRPYYADILFVTIPSENMLEFNLTNEKLILFSAKALQVKHLIDNFINEIKKDSDYVIAERNFVTDDRSMLSFHKGDIIRLQVMDGLQKYSYGCVVRKKVVFLEELKRDTEDFWKFGAVFGRSGAFPTECVHPVAPPDFQSLPLDRKAEPRGGADQFAVSSAIAVAVASTMAAHEIDQTIELDGFPDGEPDERALQDSKYDMIEFAKKYFNPLTESLIEFTDVAMNRVAADLYLIMRFMGDSPSRGSSEQEVVNMFLKMRDEAYCQLLKQLTANTSSKDSCQRGWRLLYILAAYHRCSEVLKPFLLKYLQQASRIAKACEQNLKKTFVYGGRIVPPNGMELKAMMAGRSSKRQLFLFPGGIERHVKIKTCTVALEVIEELCYEMGLHTLEAIEEYAIFLVTNRQNVRPLNKNEYILDVATEAESVDSGYSLWFRRVVWTQSLRFENELSVAMQYNQILPDYQKGLLNVLSHGKVSDQQFHQMSKLAALQHRAKDNIYTPSHELSEYIAAPLFKKQPPQQWVTMATQHMQQVQPLSPHQARAQFLLISAFPMFGSSFFYIHSSSSTTFYAPCILAVNPRGLHFLHKNTHELMAAVPLVEVQGSRTQRPSAGTSYPYVDLTVGDMNTQRVIQLQLEQQGLELCRVIAMQVENMMSVREKRLTLPPSEITML</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGN4</accession>
    <name>A0A3B5LGN4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGN4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007112.1">
        <property type="protein sequence ID" value="ENSXCOP00000007024.1"/>
        <property type="gene ID" value="ENSXCOG00000005427.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163767"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF854">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="22"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="139"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="311" mass="35601" checksum="1CBD394E2523847F" modified="2018-12-05" version="1">MSSLYNTSVLQLSGYDLPQEGVFPAFLFATLNYMIILFCNLTIIFTIVLNKSLHQPMHLFLVNLPINDLIGSSALFPHLIKEILTNSRRIQFSACIIQAFFIHIYAAGSVFILTAMAYDRYIAICYPLRYNTVMTNAHIMKIIILVWSSCVVLIAVLFILLLRLYRCRSEITHSYCDNPSLLTLVCEDTTINNIYGLFISAFTQVIANGLVFYTYLRILIACFRSRQSDTKAKALQTCATHLFVFLLFECLGLFTIISYRLQNISPHLRRFMGVSTLIFPPTLNPIIYGLKTKEIHEKVLNFFKNKLILSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJ02</accession>
    <name>A0A3B5LJ02_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJ02"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012063"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012201.1">
        <property type="protein sequence ID" value="ENSXCOP00000012063.1"/>
        <property type="gene ID" value="ENSXCOG00000009129.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182879"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF301">
        <property type="entry name" value="CYP2J25 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="147" mass="17026" checksum="244FE2638242C268" modified="2018-12-05" version="1">FIDRPKIPLFDDLIQRMGNIIPLNVPRTANQDITVDKYSIPKGTIILSILDSVLHDESMWETPYTFNPQHFLEKDGKFRKREAFLPFSAGKRVCLGEQLARMELFLFFTSLLQRFKFSPPPGEKPSLEYKLGVTHCPKPYRLCAVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LK01</accession>
    <name>A0A3B5LK01_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">sphingolipid 4-desaturase</fullName>
            <ecNumber evidence="3">1.14.19.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the fatty acid desaturase type 1 family. DEGS subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.17" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LK01"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009931"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010049.1">
        <property type="protein sequence ID" value="ENSXCOP00000009931.1"/>
        <property type="gene ID" value="ENSXCOG00000007536.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013448"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042284">
        <property type="term" value="F:sphingolipid delta-4 desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006667">
        <property type="term" value="P:sphinganine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03508">
        <property type="entry name" value="Delta4-sphingolipid-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011388">
        <property type="entry name" value="DES1/DES2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013866">
        <property type="entry name" value="Sphingolipid_d4-desaturase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879">
        <property type="entry name" value="SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879:SF21">
        <property type="entry name" value="SPHINGOLIPID DELTA(4)-DESATURASE_C4-MONOOXYGENASE DES2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08557">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017228">
        <property type="entry name" value="Sphnglp_dlt4_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01269">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingolipid delta4-desaturase N-terminal" evidence="12">
        <location>
            <begin position="5"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR017228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="321" mass="37323" checksum="E1D1D08E8D0F6C7B" modified="2018-12-05" version="1">MGKTGGRADFEWVYTDQPHTARRKEILAKYPEIKTLMGPDPQLKWVVSGLVLTQLVACYLVHDLPWKWVFFWAYGFGGCINHSLTLAIHDISHNVAFGNKLAKWNRWFAMWANLPIGLPYSASFKKYHIDHHRYLGGDQLDVDIPTEWEGWFFCTPARKVLWLFLQPFFYALRPLVVNPKPVGPMEIQNTVVQLLADAVIFYFWGLKPIVYLIAGSILCLGIHPFSGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHMEHHDFPSIPGSKLPQVKKIAAEYYDSLPQHTSWFQVLWDFVFDENIGPYARVKRDVNRLHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKC3</accession>
    <name>A0A3B5LKC3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FGF</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKC3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012523"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012674.1">
        <property type="protein sequence ID" value="ENSXCOP00000012523.1"/>
        <property type="gene ID" value="ENSXCOG00000009467.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000173884"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="69" mass="7842" checksum="29FBC6A03C754E5D" modified="2018-12-05" version="1">MSGQGLAYGEEQFSDNCLFKESVEENHYTTYSSMFHLGNYLAISHRGQLRRGSSVSPNQSCAHFLPRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKH1</accession>
    <name>A0A3B5LKH1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Dicarboxylate carrier UCP2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Mitochondrial uncoupling protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Solute carrier family 25 member 8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Antiporter that exports dicarboxylate intermediates of the Krebs cycle in exchange for phosphate plus a proton across the inner membrane of mitochondria, a process driven by mitochondrial motive force with an overall impact on glycolysis, glutaminolysis and glutathione-dependent redox balance. Continuous export of oxaloacetate and related four-carbon dicarboxylates from mitochondrial matrix into the cytosol negatively regulates the oxidation of acetyl-CoA substrates via the Krebs cycle, lowering the ATP/ADP ratio and reactive oxygen species (ROS) production. May mediate inducible proton entry into the mitochondrial matrix affecting ATP turnover as a protection mechanism against oxidative stress. The proton currents are most likely associated with fatty acid flipping across the inner membrane of mitochondria in a metabolic process regulated by free fatty acids and purine nucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(S)-malate(out) + phosphate(in) + H(+)(in) = (S)-malate(in) + phosphate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:73299"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(S)-malate(out) = (S)-malate(in)</text>
            <dbReference type="Rhea" id="RHEA:74555"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>H(+)(in) = H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34979"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-aspartate(out) + phosphate(in) + H(+)(in) = L-aspartate(in) + phosphate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:73307"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-aspartate(out) = L-aspartate(in)</text>
            <dbReference type="Rhea" id="RHEA:66332"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a long-chain fatty acid(out) = a long-chain fatty acid(in)</text>
            <dbReference type="Rhea" id="RHEA:39283"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>malonate(out) + phosphate(in) + H(+)(in) = malonate(in) + phosphate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:73387"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15792"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>oxaloacetate(out) + phosphate(in) + H(+)(in) = oxaloacetate(in) + phosphate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:73303"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>phosphate(in) = phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:32823"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>sulfate(out) + phosphate(in) + H(+)(in) = sulfate(in) + phosphate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:73391"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotetramer. Adopts an asymmetrical dimer of dimers functional form.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 27">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKH1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012568"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012719.1">
        <property type="protein sequence ID" value="ENSXCOP00000012568.1"/>
        <property type="gene ID" value="ENSXCOG00000006213.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165592"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000008">
        <property type="entry name" value="Mitochondrial uncoupling protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00784">
        <property type="entry name" value="MTUNCOUPLING"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 26 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 27" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="26">
        <location>
            <begin position="11"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="26">
        <location>
            <begin position="115"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="26">
        <location>
            <begin position="216"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00050147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00050345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00050909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00052608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00054589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00074760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="318" mass="34607" checksum="8E419C7757D2D2E2" modified="2018-12-05" version="1">MAGGSAADLGPTAVVKVFSAGTAGCVADLVTFPLDTAKVRLQVQGESKALPKGQKATYRGVFGTIITIVRTEGPRNLYNGLLAGLHRQMCFASVRIGLYDTMKQLYAHGAESMYPGLGTRLLAGSTTGAMAVAFAQPTDVVKVRFQAQVHQPENGSVKRYSSTTEAYRTIARVEGLKGLWKGCLLNIARNAIVNCSELVTYDIIKEQILKNKLMTDNMPCHFTAAFAAGFCTTVVASPVDVVKTRFMNSVPGQYGGAANCALTMLLREGPSAFYKGWVSFMPSFLRLGSWNIVMFVSYEQIQRVVVRFQRRLASERFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKK7</accession>
    <name>A0A3B5LKK7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKK7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010126"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010245.1">
        <property type="protein sequence ID" value="ENSXCOP00000010126.1"/>
        <property type="gene ID" value="ENSXCOG00000007681.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005224"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031461">
        <property type="term" value="C:cullin-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019138">
        <property type="entry name" value="De-etiolated_protein_1_Det1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13374:SF3">
        <property type="entry name" value="DET1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13374">
        <property type="entry name" value="DET1 HOMOLOG DE-ETIOLATED-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09737">
        <property type="entry name" value="Det1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="524" mass="60792" checksum="3FCBB523E8846799" modified="2018-12-05" version="1">IATMEEDVLTLKPRRIQNQNVVHRLEKRRVCSGRPGAHWYRVRCFHQNLFPNFTVVNVEKPPCFLRKFSPDGRYFIAFSSDQTSLEIYEYQGCQAAQDLLRGQEGETLSTANDQCSLNIRSRLFQRFFSLLHVTNVASNGEHLNRECSLFTDDCRYVIVGSAVYVPDDPPPYFFEVYRNNESVTPNPRSPLEDYSLHIIDLHTGRLCDTRSFKCDKIILSHNQGLYLYRNILAVLSVQQQTIHVFQVTPDGTFLDVRTIGRFCYEDDLLTLSAVYAEAQAESQTGFPRLYTDKTINSLKHRLLVYLWRRAEQDGSATAKRRFFQFFDQLRRLRMWKMQLLDEHHLFIKYTSEDVVTLRVTDPSQPSFFVVYNMVSTEVLAVFENTSDQLLELFENFCDLFRNATLHSQAVQFPCSASSNNYARQVQRRFKDTIVNAKYGGHTEAVRRLLGQLPISAQSYSSSPYLDLSLFSYDDKWVSVMERPKTCGDHPIRYAKMQLCSFQVNIHPSAVCFHVFHSSNDSIVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKZ1</accession>
    <name>A0A3B5LKZ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKZ1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011729.1">
        <property type="protein sequence ID" value="ENSXCOP00000011597.1"/>
        <property type="gene ID" value="ENSXCOG00000008743.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166826"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005332">
        <property type="term" value="F:gamma-aminobutyric acid:sodium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11496">
        <property type="entry name" value="SLC6sbd-TauT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF249">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 6 MEMBER 22, TANDEM DUPLICATE 2 ISOFORM X2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="201"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
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    <feature type="transmembrane region" description="Helical" evidence="10">
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    </feature>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    </evidence>
    <sequence length="616" mass="70089" checksum="0074288491C2C9CF" modified="2018-12-05" version="1">MDPKQTSHVKTKPNPGERPFSIEPKTHVRDQWATKMEFILAVAGHIVGLGNVWRFPYLCYKNGGGVFFIPYVLFLFTCGIPLFFLETSLGQFTSQGGITCWRNMCPLFEGLGYGSQVVVLYTGVYYIVILAWTFLYLFSSFRTELPWVSCNNSWNTVACFEHGHNDTSSFHLYANTTSSVVQFWERRILGLSNGIDNIGSVRWDLALCLLLAWVLCYFYIWNGVKSTGKVVYFTATFPYVMLAVLLVRGLTLPGAKDGILFYLYPDPSRLADPEVWMDAGSQIFYSYGVCTGVLTSLGSYNKYNNNCYRDCIYLCLLNSLTSFVAGFAIFSVLGFMAKEQNVEISMVAESGPGLAFIAYPRAVALMPLPQLWAIFFLFMIIFLGLDSQFVYQESLVTTISDMYPSFFQKTLLRRLLLLTICAGGFLVGLVMVTEGGLYVFQLFDYYACSGMTLLFFALLQSICIGWVYGADRHYDNVKDMIGYRPWPFMKYCWRYITPAICTFTFLFSVIKHKPLKFNNTYEYPWWGYAFGLFFTLSSTLLVPLWMVYAVSITPGTLKQVNILNFEIILLIRHQGWEGTGVSDHSAFLPAHSTSIYGKRYNRGIGKVWKREGGIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLP2</accession>
    <name>A0A3B5LLP2_9TELE</name>
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        </recommendedName>
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        <name type="scientific">Xiphophorus couchianus</name>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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    </evidence>
    <sequence length="105" mass="12369" checksum="5D62A493577048A5" modified="2018-12-05" version="1">MKIILKFVSLLISFILIMIFVAFFILSERKILGYIQLRKGPKKVGVFGLLQRFSDLIKLLVKFKILFFQYRTLIAMLGTLLIILFSLIFCSLCINYFLTKNLKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLP8</accession>
    <name>A0A3B5LLP8_9TELE</name>
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        <recommendedName>
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        </recommendedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
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            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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    </organism>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="HD"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
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    <keyword evidence="4 12" id="KW-0805">Transcription regulation</keyword>
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            <end position="384"/>
        </location>
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            <begin position="955"/>
            <end position="1015"/>
        </location>
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            <end position="1016"/>
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            <end position="236"/>
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        </location>
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            <end position="442"/>
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            <end position="640"/>
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            <end position="819"/>
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            <end position="1070"/>
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            <begin position="1103"/>
            <end position="1134"/>
        </location>
    </feature>
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        <location>
            <begin position="1155"/>
            <end position="1252"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
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        <location>
            <begin position="173"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="190"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="277"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="290"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="568"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="741"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="770"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="784"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="809"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1028"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1108"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1185"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1196"/>
            <end position="1219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1277" mass="139264" checksum="FFAC2B4E2EED8A13" modified="2025-10-08" version="2">MNAAQTELLELRCKYNQEMANKAEVDAIVANLEKANQRAEMAQREVERLKEQLASGPATVEAALLAKDREILRLLENIQRLQFTLQEVQETSSNQILELERQLAYKTEAIERLEAKLQSQMDYEEIKTELILKVMKLASANGSSSQDSAKAAEALLLDKDVFLPSHKYMGDKTRLLHDDRSDNTGKEASRPPGSHSSSSQAESRASPSPGPPTLNGSSSGHDLPRPFSASPCSGDRMSGDHILHKQLLSPHFKKEGIMAFPTALYAAKVALMSASQGSAGAGSMDAGLPSDQSESGSSTAGDEDQLDTAEIAFQVKEQLLKHNIGQRVFGHYVLGLSQGSVSEILARPKPWRKLTVKGKEPFIKMKQFLSDEQNILALRTIQVRQRSITPRIRTPETGSDDAIRNILEQAKKEIQSQRGDGKSSLSSSSGRSSNGAGSSSDETIKNILEQARREMEAQQQTLMEMEACGRSSAASSGVQVERLGPPERSRVLPLPISIKQEDGGCIAVCMANSISSPQTPLSVLSPAAFVQNIIRKVKSEIGEAGTYFDQHWSQERGAMILSGGGGSSRPFSSVSPSLSSSSSGPPTLSRTWPRLENGDGLANSEEVSNADDELGLGRPEVKVESDTSVSGESPGPSPGRLSYYPAYIPRALKPTVPPLTPEQYEMYMYREVDTLELTRQVKEKLAKNGICQRIFGEKVLGLSQGSVSDMLSRPKPWSKLTQKGREPFIRMQLWLLDQLGQSLSQPPNQGHTQKSPVTAQSCPSPPPSPAESNPSPVAEPVSLSLESSKENQQPQSLSLGLPSHPEGGKSTSSLMGLHQPTTPLGIQELVAMSPELDTYAITKKVKEVLTDNNLGQRLFGETILGLTQGSVSDLLSRPKPWHKLSLKGREPFVRMQLWLNDPHNVEKLRVMKKMEKKYLKRRYGLLSTGSDSDSPSTRSECVSPALASLDLCPFNQVKKPRVVLGAEEKEALRKAYMQEPYPSQNTIEMLASQLNLKTNTVINWFHNYSRMRREVLMGGLPDNDTDAEPNSYSPSVTRSPNSDGEDKRLLLPSGQTHPGLPLSTTDALPHVKQEATYREEDSMDGREGSQKEVGVQCFPATMPLSPLKTEHEDSASGCREPHPAGQSPTQEEGARNQIQGLHLGAASADGLQRANKSRYDGEESGESPIDPVSFKATSEPCRSSLEVSLNSPSAASSPGLMMSVSPVPSSSAPISPSLPNQATSANHGMDANPLPPFQSPKLNRSTQRRNEKMANLNNIIHRLERAANREETLEWEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLY9</accession>
    <name>A0A3B5LLY9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRM IMP/VICKZ family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLY9"/>
    <dbReference type="SMR" id="A0A3B5LLY9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013243.1">
        <property type="protein sequence ID" value="ENSXCOP00000013083.1"/>
        <property type="gene ID" value="ENSXCOG00000009912.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154957"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22490">
        <property type="entry name" value="KH-I_IGF2BP1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22493">
        <property type="entry name" value="KH-I_IGF2BP1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22496">
        <property type="entry name" value="KH-I_IGF2BP1_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22499">
        <property type="entry name" value="KH-I_IGF2BP1_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.210:FF:000001">
        <property type="entry name" value="insulin-like growth factor 2 mRNA-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000026">
        <property type="entry name" value="Insulin-like growth factor 2 mRNA-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000027">
        <property type="entry name" value="insulin-like growth factor 2 mRNA-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="K Homology" evidence="13">
        <location>
            <begin position="65"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="13">
        <location>
            <begin position="146"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="13">
        <location>
            <begin position="274"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="13">
        <location>
            <begin position="356"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="22"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="436"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="453"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="468" mass="50530" checksum="A2BB433741A5B1E6" modified="2018-12-05" version="1">IAIQKLNGYQLESNALRVSYIPDETSDMEGGQRGTENSRRPGYGPRGGLRGGSPSSGMPPKPPHADIPLRLLVPTQYVGAIIGKEGATIRNITKQTQSKIDVHRKENAGAAEKPISIHSTPEGCSAACRMILEIMHQEAKDTKTADEVPLKVLAHNNFVGRLIGKEGRNLKKVEQDTDTKITISPLQDLTLYNPERTITVKGSIEACCKAEVEIMKKVREAYENDIAAMNQQTHLIPGLNLGALGLFPPSSNMPPPPPGNAVGGTPYGSFGAPEQETVHVYIPAQAVGAIIGKKGQHIKQLSRFAGASIKIAPAETPDSKMRMVIVTGPPEAQFKAQGRIYGKLKEENFFGPKEEVKLETHIKMAAAAAGRVIGKGGKTVNELQNLTAAEVVVPREQTPDENDQVIVKINGHFYASQLAQRKIRDILTQVKMSQKGGMGMGPGPHPQGFTEMGSPTQGLTQDHQPRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQ65</accession>
    <name>A0A3B5LQ65_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FHOD1 N-terminal GTPase-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQ65"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013080.1">
        <property type="protein sequence ID" value="ENSXCOP00000012920.1"/>
        <property type="gene ID" value="ENSXCOG00000009779.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160212"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041387">
        <property type="entry name" value="FHOD1_GBD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920:SF2">
        <property type="entry name" value="FH1_FH2 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920">
        <property type="entry name" value="FORMIN HOMOLOGY 2 DOMAIN CONTAINING, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18382">
        <property type="entry name" value="Formin_GBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHOD1 N-terminal GTPase-binding" evidence="1">
        <location>
            <begin position="5"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="77" mass="8414" checksum="9C8B8B9DA4B33B2C" modified="2018-12-05" version="1">MDSVTCRVQYLEDSDPFICTNFPEPRRPPTVSLEEDQPLSEQISGIHKLLGAPLKVGHTPNRAGTGLSVSLVPKSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQE5</accession>
    <name>A0A3B5LQE5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial nucleoid factor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Mitochondrial protein M19</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
            <location evidence="1">Mitochondrion nucleoid</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027858292.1">
        <property type="nucleotide sequence ID" value="XM_028002491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQE5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011636"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011769.1">
        <property type="protein sequence ID" value="ENSXCOP00000011636.1"/>
        <property type="gene ID" value="ENSXCOG00000008788.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114135315"/>
    <dbReference type="KEGG" id="xco:114135315"/>
    <dbReference type="CTD" id="84300"/>
    <dbReference type="GeneTree" id="ENSGT00510000048041"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037698">
        <property type="entry name" value="UQCC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34260">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME-C REDUCTASE COMPLEX ASSEMBLY FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34260:SF1">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME-C REDUCTASE COMPLEX ASSEMBLY FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20180">
        <property type="entry name" value="UQCC2_CBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1135">Mitochondrion nucleoid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="96"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="101"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="130" mass="15135" checksum="156C7C56B80FACF6" modified="2018-12-05" version="1">MSATRYRRFLKLCEEWPRDETKKARDLGTFLRQRVASAFREGENTQLADPEKCDQMYESLARINGNAYRQRFPRVRDTSFTGVTAEECRVLLSGSMQQSMDEEKKGLWKSLMERFSSKSPEDGPEKAPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQL0</accession>
    <name>A0A3B5LQL0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQL0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014398"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014582.1">
        <property type="protein sequence ID" value="ENSXCOP00000014398.1"/>
        <property type="gene ID" value="ENSXCOG00000010926.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009628"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050477">
        <property type="entry name" value="GrpII_AminoAcid_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735:SF1">
        <property type="entry name" value="PYRIDOXAL-DEPENDENT DECARBOXYLASE DOMAIN-CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="158" mass="17166" checksum="3BB4F0ED98F6264B" modified="2018-12-05" version="1">MTLTPGRWLGLPAVTAVTLYRHEDPALSLAAGLTSSQPVEKLRALPLWLSLQYLGHNGIVQKIRHAAALVRMTFSETTVRRMTVFRHVGEPSPNPATGAQQNLLEPSAGQGICSLTVSVRCVRGFLCIVVSTGYLGSRARCRRSHLSLGLLCGTFWTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRV7</accession>
    <name>A0A3B5LRV7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">E3 ubiquitin-protein ligase NEURL1</fullName>
            <ecNumber evidence="7">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="27">Neuralized-like protein 1A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">RING-type E3 ubiquitin transferase NEURL1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="33 34">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Plays a role in hippocampal-dependent synaptic plasticity, learning and memory. Involved in the formation of spines and functional synaptic contacts by modulating the translational activity of the cytoplasmic polyadenylation element-binding protein CPEB3. Promotes ubiquitination of CPEB3, and hence induces CPEB3-dependent mRNA translation activation of glutamate receptor GRIA1 and GRIA2. Can function as an E3 ubiquitin-protein ligase to activate monoubiquitination of JAG1 (in vitro), thereby regulating the Notch pathway. Acts as a tumor suppressor; inhibits malignant cell transformation of medulloblastoma (MB) cells by inhibiting the Notch signaling pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 35">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="25">Interacts with CPEB3 (via N-terminal domain); the interaction increases CPEB3 ubiquitination. Interacts with DLL1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Perikaryon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="23">Postsynaptic density</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRV7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013535"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013702.1">
        <property type="protein sequence ID" value="ENSXCOP00000013535.1"/>
        <property type="gene ID" value="ENSXCOG00000010255.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156696"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000022">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEURL1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000005">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase NEURL1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000056">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase NEURL1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037962">
        <property type="entry name" value="Neuralized"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006573">
        <property type="entry name" value="NHR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12429:SF13">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEURL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12429">
        <property type="entry name" value="NEURALIZED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07177">
        <property type="entry name" value="Neuralized"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00588">
        <property type="entry name" value="NEUZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51065">
        <property type="entry name" value="NHR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="21" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="22" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0519">Myristate</keyword>
    <keyword evidence="18" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="19" id="KW-0770">Synapse</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="16" id="KW-0862">Zinc</keyword>
    <keyword evidence="14 29" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="NHR" evidence="32">
        <location>
            <begin position="48"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="NHR" evidence="32">
        <location>
            <begin position="268"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="31">
        <location>
            <begin position="497"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="30">
        <location>
            <begin position="432"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00034105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00053982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00063138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00073400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00078614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00082012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS51065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="550" mass="60286" checksum="E8895C3750B13B18" modified="2018-12-05" version="1">MFITHFMFVFSDSLGGPFPSTSHRCHHKSKRCLPIQCGSVGGPFPVSPLLFHPNTKGSQIVMDLAQKTVKRQASFCNAITFSNRPIALYEQVRLKITKKQCCWSGALRLGFTSKDPSRINPDNLPKYACPDLVSQSGFWAKALPEEFANEGNIIAFWVDKKGRVFYRINESSPMLFFSGVRTAEPLWALIDVYGLTRGVQLLDSEIVPPDCLRPRSFTTVRSSSLRREADDSRLSVSLCDLNLQQEDRGPNSLNSQQSSLLPSALESDLHFHQLRGAHIKTLDEQTVARSEHAREERTLVFTNRPLRTGETVFIKVTKSSPVRSGSLSYGVTSCDPAVLRPSDLPYNPEALVDRKEFWAVCRVPTPLQSSDILGFLVNQEGEVILSHNGTNVGMQVCVDNSRPLWMFFGLHGAVTQLRILGSTHAGDPRATSNPGSPSSSPHTPSALGSGISDPVLNGGLCSAAYCSSAGGTTPSSPVSLPKSPTFPSGRGPWADECSICYENAVDSVIYTCGHMCLCYACGLKLKKMSNACCPICRRQIKDIIKTYRST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LT21</accession>
    <name>A0A3B5LT21_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribonucleoprotein, PTB-binding 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LT21"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010954"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011079.1">
        <property type="protein sequence ID" value="ENSXCOP00000010954.1"/>
        <property type="gene ID" value="ENSXCOG00000008271.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158648"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000100">
        <property type="entry name" value="Putative ribonucleoprotein PTB-binding 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000125">
        <property type="entry name" value="Putative ribonucleoprotein PTB-binding 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="80"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="153"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="315" mass="35633" checksum="EB3CCA1A5E82E2EC" modified="2018-12-05" version="1">MAAASSIQPPSVHSSLNRDSVLSPDMDSPESRQPEDEEAAGAAAAQPSEPGVSLRDLPDLDPEEIERRLAKTRRELSNRRKILIKNLPPDTSNQEVHEILKEYELKYCFVDRNKGTAFVTLLNGDQAQDAIRSLHHSTVRGRLINVTLQPTDSLLCLTNLPHTFTAQQFEELVRLYGNIERSFLVYSELTGHSKGYGFVEYMKKDSASRARSELLGRPLGDRSLMVQWMDVNQLSQEENLHSKCLCVDRLPLDLCDSEELAQLFSETYKPVFCQVGFSHCFAETKHPHIILPNVTFLCMDHCLALSNDYYVKIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTG6</accession>
    <name>A0A3B5LTG6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">IRS-type PTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTG6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014125"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014302.1">
        <property type="protein sequence ID" value="ENSXCOP00000014125.1"/>
        <property type="gene ID" value="ENSXCOG00000010701.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155980"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050996">
        <property type="entry name" value="Docking_Protein_DOK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258:SF46">
        <property type="entry name" value="DOCKING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21258">
        <property type="entry name" value="DOCKING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01244">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00310">
        <property type="entry name" value="PTBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51064">
        <property type="entry name" value="IRS_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRS-type PTB" evidence="2">
        <location>
            <begin position="133"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="422"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="434"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="462" mass="50782" checksum="326F5CB8B3606E73" modified="2018-12-05" version="1">MDSHIKTGKVYLKPHKPHKKFSKFSKSDQFCVCAAGGADLRKKVKVVRLSELISVLRLPPNAEACHLDNMSTFCVETEDRTLVFAAPKDDCLEWVDKLRHIQNLSASVLQREKSSGSSQVHMEENQIYASADEAPLFWVVVQKNDAATRCGLQGSYWLQLEQQSLLLRDTKTKVVEWPYELLRRYGHDKVSFTVEAGRRCESGPGTFCFETQQADKIFNLIQSNIKRNALAAASGSQSQDTEKLRMQDRRSPVFDNMAKGLDEVGASDGSPAPITLMPLPSIPTLDGTSRDKIKSQSDAIYADPNVCGPSIVVPKLAIAQYIDPAKVLPLKPPFSLDSGAALPTASDSKPDTKASIPDSVYSEVYDKISPDKNKQPVLQNATKTSDEPIYSEPVSRKESDCQKPEPKPDPFAHLYAQVCKAPTSSRNSAPVTMTTTSSATTASSTSIEEPLDDVIFENLGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LV98</accession>
    <name>A0A3B5LV98_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">REX1, RNA exonuclease 1 homolog</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO1/REXO3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LV98"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011759"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011894.1">
        <property type="protein sequence ID" value="ENSXCOP00000011759.1"/>
        <property type="gene ID" value="ENSXCOG00000008878.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165897"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="REX1_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RNA exonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="REX1-like_exo"/>
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    <dbReference type="InterPro" id="IPR031736">
        <property type="entry name" value="REXO1-like_dom"/>
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        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF62">
        <property type="entry name" value="RNA EXONUCLEASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EloA-BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="8">
        <location>
            <begin position="1059"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="38"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="100"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="162"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="240"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="640"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="778"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="100"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="187"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="242"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="296"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="320"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="352"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="375"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="390"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="403"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="511"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="545"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="567"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="594"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="644"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1234" mass="137695" checksum="7BE23BAA3226E767" modified="2018-12-05" version="1">MLRSTGFFRGIDCPFYADYNDGKGSRNGCNRPYCHFRHSRQSRPSYGDVKKLQSGQKDQGYDPFNPEVVRPQEQQNGQPAPSGDLSGALEMVNRAIEEVRGEVEREQRKLSRIGDEPYSPTEKTGSSSSYVAKNQAAASHLAYDPGSYQITSGGYDPTPGCSKYTLDSDSQKYNSSSSMEYVPTSLKKPQSRTQVPCPPQSTKYLSTACSAQWKYTLDNSGPSTNMEYDPLSNYSAGIAVKNKRDEGSHPVETDYKKAYRLDASDKEGGTAGKKPPQASIDLKKYTISDSDEESSGTEYRPTSLSNLQQRKANIVSARDALGKDTKDRSKSIKGTLTQRNKEELAQDLDSQENTKQKENLEKRKLADQSGHGKISKPEKTQNIDKEVKKSSSVKSSSGSSSSAKDKLSCKNFKQDSAKKENKNQKDDSKNMLKMKDKVQREGRDEKRCESKSKMVEKVKRALSEQEKDARESKKLKPSEREKDQSKNKDSQHRNGKLDSSRREKDVKKSKSSSSSCKGSSAKAKQSASSSKEKLEDLSLSHADLFGDESPEEADPMEVDSDDEPEEVLVRKSADALKRGLMSKRTASERTPSSSEDKAEGEPERSRSNWEEPDDVRVDFSMFQDDLDFDSDPMEECLRIFNESNDVKKEDKGRQAKQASRDSEEERSTDSTLTTLFPGQKKRVSHFTDAPSKTVVRPYKRPTPQEVCFQRMQLAQQQAAQLSASVKAASQSSTSSFAGEKKRIAHRPNPQITSTKTSRIETPQGVKSQTTAGILSKTSSTMAQKRTAHTPTMKSSSMRRPVIPTEFGAKVPTNIRQRYLNTFIDECVKFCPSEDVAFQTALDEEKLVYERSSSKNIYLNVAVNTLKKLRGKSSSCSSPVKSMLAAKRKAQSHEEVLGGRLAATTSFTVNRSGKHQEEKLSGANLYRKLQAYLMTEEQLQEHGYPRLNPEAPGKAVIYNQPERKICPDPFGRICCRCGAEYKVSVNGSCVRREECSYHWGRLRRHRVAGGWETTYSCCSAAVGAPGCQISKQHVQDGRKESLDGFVSTFSKPLPPDGNGGVFALDCEMCYTKQGLELTRVTVIDSEMKVIYDTFVKPDSKVVDYNTRFSGVTEEDLVNATITLRDVQAVLLSMFRAESVLIGHSLESDLLALKLIHSSVVDTAIVFPHRLGLPYKRALKTLMADYLKRIIQDNGSMRRNSISIMKWKSPNQAKLGEIFQLSVIFTDIDLKYTVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVI0</accession>
    <name>A0A3B5LVI0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ITPK1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVI0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011839"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011975.1">
        <property type="protein sequence ID" value="ENSXCOP00000011839.1"/>
        <property type="gene ID" value="ENSXCOG00000008953.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001278"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052726">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052725">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047325">
        <property type="term" value="F:inositol-3,4,5,6-tetrakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032957">
        <property type="term" value="P:inositol trisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11370:FF:000001">
        <property type="entry name" value="Inositol-tetrakisphosphate 1-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008656">
        <property type="entry name" value="Inositol_tetrakis-P_1-kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040464">
        <property type="entry name" value="InsP(3)kin_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041429">
        <property type="entry name" value="ITPK1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14217:SF1">
        <property type="entry name" value="INOSITOL-TETRAKISPHOSPHATE 1-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05770">
        <property type="entry name" value="Ins134_P3_kin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17927">
        <property type="entry name" value="Ins134_P3_kin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Inositol-tetrakisphosphate 1-kinase N-terminal" evidence="11">
        <location>
            <begin position="9"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Inositol 1,3,4-trisphosphate 5/6-kinase ATP-grasp" evidence="10">
        <location>
            <begin position="120"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Inositol 1,3,4-trisphosphate 5/6-kinase ATP-grasp" evidence="10">
        <location>
            <begin position="193"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="360" mass="39916" checksum="B5573EA12681E5BF" modified="2018-12-05" version="1">MQTFLRGRRVGYWLSEKKMKKLNFQAFADLCRKRGIEVVQLDLSQPLEDQGPLDLIIHKLTDLILEADQNDSQAVLQVQRVQDYIDAHPETIVLDPLPAIRTLLDRCKSYQLVHRIEKCMQDERICSPPFMVLNTECTPDVLEQIKLQGLTFPFICKTRVAHGTNSHEVRSCTSCRDVKLIFCFSLFLLLHLSERKAIFFNSHNVSKPESSSDLTSRENVEGVSQPPCDDVIRELSRSLRQALGVSLFGIDVIINNQTGQHAVIDINAFPGYEGVPEFFNDLLSHISGVLQSHNPDFVPAGDQPKGLTVSGGAGGAGPQAPCGCSSVRGKELQRLGCNSAMSPNFQQHCVSTIATKASSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LW51</accession>
    <name>A0A3B5LW51_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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        <property type="component" value="Unplaced"/>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWP3</accession>
    <name>A0A3B5LWP3_9TELE</name>
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        </submittedName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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        <scope>IDENTIFICATION</scope>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF96">
        <property type="entry name" value="RAS PROTEIN ACTIVATOR LIKE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25321">
        <property type="entry name" value="PH_RASGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="307"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="4">
        <location>
            <begin position="550"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="387"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="139"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="396"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="977" mass="108411" checksum="3314E57823166043" modified="2018-12-05" version="1">MGTEEKDSEHAAHPLSKEQGSPEKEKETPVEHPQLSETADPLNTYKWHTGSKGTLNEPREEGDGAAATSTSTIDKIQKASSNKWSKMQNWRKALSEDPGDKNSTGGKSGEATKVEKGAGATRKNPFRRALSEPPGSLFAALTPSSSSASSAQPASSSAAPEAAGASSNDPSSKGSGGTIFKKYLWNVTQKLKRPRLQSRNSEHVPPQEVPLWDITNCILGDGQILITQEDECSPDPVGLPGGSRPKNQDGGVRGLIKRRLDKAKRNSTTQLQYGSRESVSVPVGSLDLSADTSTVIRPVHSSILGEKYCFEVINSENTHCFGCSSAAERDRWIEDLRRAAQPNKDNIERTENSLSLWVNEAKDLPPKRRYYCEVHLDGTLFARTSSRAVGKPTNRSSMAGDSASGSTGGLGGSGGAAGGCQLFWGEFFELDNLPSISQISLHLFREEDAKKKRHSRDESSLHPLGSVNIPLADIRGRTYQEKWYPITPFKASASAGNKEVLSSQASLRIKARFQNLKVLPMEKYKEFAEYVTVRYVKMCGNLEPLLNVKEKEELAGALVHVLQSIGKAKEFLIDLGDAEVNRLGEKEALIFRENTLATKAIDEYMKLVGQKYLIDTLDFINRLYASVENCEVDPLKCPAAELSSNQQHLRETCEDVVQKIIEIHSFPEELNKIFSNWMELCEDKGKAEIGQRLISASLFLRFLCPAILSPSLFGLTQAYPEPNTLRTLTLTAKVIQNLANSTFGEKEEYMLFMNEFLDQHWDGMKCFLQTVSNQDTEIPMTSFDGYVDLPLRLAVLHGLLVDIIYKKNQDTIDKLHPLPSILNQISDSLGPEAPRIIVNHTKPSKPVYVPPRELRKYSPLQSSLQRLPVDSKHAQELSELRLGIEQVTERELDMAKRLEDFIIQSQDQNAMLQAEVYELKNLLAVREEQLASATFLGVIEEEREEDERRLGVALAAAERMNVLEEQFAGLTKDLQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LY65</accession>
    <name>A0A3B5LY65_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">adenylate cyclase</fullName>
            <ecNumber evidence="5">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LY65"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016848"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017065.1">
        <property type="protein sequence ID" value="ENSXCOP00000016848.1"/>
        <property type="gene ID" value="ENSXCOG00000012712.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159096"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007193">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000012">
        <property type="entry name" value="Adenylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032628">
        <property type="entry name" value="AC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF9">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16214">
        <property type="entry name" value="AC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="15 16" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="17">
        <location>
            <begin position="227"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="476" mass="54581" checksum="1CBC075AB5AEC5D3" modified="2018-12-05" version="1">LLQETKEHRAFVSVVSSSLCVFVIVLIMVCTELFSQRLRFLLGVVIWATHLIMGYAFIFSKAVILPWDQVPFFLFIIFTVYTMLPFQLWHAVVLSVITSLSHIMILTLRLTSDDTPKDYLANQLLSNAVVFLCGCGVGAFHKVLMEKTLRQTFQDTLRCLSMRVKLEIEKRQQENLLQSVLPVYISMKMILAIMERLQDCKDKVEQQRLVKDNNFHSLYVKRHENVSILYADIVGFTRLASDCSPKELVHMLNELFGKFDQIAKENECMRIKILGDCYYCVSGLPVSLPMHAKNCVKMGLDMCEAIKQVRDATEIDINMRVGVHSGNVLCGVIGLRKWQFDVWSHDVTLANHMESGGLPGRVHITEATLKHLNKAYEVEEGNGHLRDSYLNELNVKTYLVIDPRSKDPSQSTRTTTKPRVNDGLKMRASVRMTRYLETWGAVKPFAHLTSLYEPHPPTPRKLIGKEKFRFLKYFAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M262</accession>
    <name>A0A3B5M262_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Adenylate cyclase activating polypeptide 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glucagon family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M262"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015661"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015855.1">
        <property type="protein sequence ID" value="ENSXCOP00000015661.1"/>
        <property type="gene ID" value="ENSXCOG00000011829.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183154"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051428">
        <property type="term" value="F:peptide hormone receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016521">
        <property type="term" value="F:pituitary adenylate cyclase activating polypeptide activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043010">
        <property type="term" value="P:camera-type eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
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</entry>
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        <scope>IDENTIFICATION</scope>
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        <location>
            <begin position="919"/>
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        <source>
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        <source>
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        </source>
    </evidence>
    <sequence length="1290" mass="145150" checksum="60002C395E2B0522" modified="2018-12-05" version="1">RLRVIFYGRAPIPMAVVRRELSCESYPIELRCPGTDVIMIESANYGRTDDKICDSDPAQMENTRCYLPDAYKIMSLRCNNRTQCAVVAGPDVFPDPCPGTYKYLEVQYECVPYIFLCPGILRGVYQSEHLFESDHQSGAWCKDPLQASDKIYYMPWTPYRTDTLTEYSSKEDFIAGRPTTTYKLPHRVDGTGFVVYDGALFFNKERTRNIVKFDLRTRIKSGEAIIANANYHDTSPYRWGGKSDIDLAVDENGLWVIYATEQNNGRIVVSQLNPYTLRIEGSWDTSYDKRSASNAFMICGVLYVVKTVYEDDDNEGTGNKIDYMYNTDKSKETTVNIPFPNSYQYIAAVDYNPRDNLLYVWNNYHVVKYSLDFGNNGKFNRGSPSITTSLLISLVTPLHWLLLFPKIIWNISTGFSYVTSVSLLLPQAMVETVNNLLHPRAQAAWKELPTSEQLHSATLLLDTVETGAFMLADNLLKTDTVQETTDYIQLEVARLSTDGNLADLTFPQSEQHGNAIQLSASTLKQHGRNGEIRMAFVLYRNLGSYLPTENASVRLSSEAVYPNYSVIVNSPVITASINKESNKVYLSEPVVFTVQHLQNFNPNCSFWSYSKRTMTGFWSTQDCRLLATNRTHTSCSCTHLTSFAVLMAQVEKADSMHDMLLDVITWVGILLSLVCLLICIFTFCFFRGLQSDRNTIHKNLCISLFIAESLFLVGINKIVCAVFAALLHFFFLAAFTWMFLEGVQLYIMLVEVFESEHSRTKYFYLAGYGVPAVIVAVSAAVDYRSYGTDRVCWLRLDTYFIWSFIGPATLIIMLNVIFLGIALYKMFHHTAILKPDSGCLDNIKSWVIGAIALLCLLGLTWAFGLMYINESTVIMAYLFTIFNSLQGMFIFIFHCILQKKVRKEYGKCLRTHCCSGKSVETSISSSSKTTTSRTPGRYSTGSQSRIRRMWNDTVRKQESSFITGDINSSATLNRGAMANHLITNALLRPHGTNNPYNTLLGDSAVYNNPAVEGILNNARDASVMDTLPLNGNHPNNFSMASSEYLSDCVQILDRSGGYGTHSNHKETTLEKKILKELTSNYIPPYLNNHERATTERSHNLMNKLVNSSMANGGNCAVIFSSLLDNPAAFPHEENLGLEIIREESNAPLLPPRFSRQRRIAQEPSESFFPLLTNEHTDEHTHSPNHRDSLYTSMPVLTDLPDGQMNGLTDGEEDSSGELQPCKSATAPELDDVYYKSMPNLGSRNHLHELQNYYHMGRGGSDGYMVSGSKEESDSSPEEPPVDPSHLVTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M426</accession>
    <name>A0A3B5M426_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Phosphoglucomutase 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027875086.1">
        <property type="nucleotide sequence ID" value="XM_028019285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M426"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018212"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018443.1">
        <property type="protein sequence ID" value="ENSXCOP00000018212.1"/>
        <property type="gene ID" value="ENSXCOG00000013719.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114145627"/>
    <dbReference type="KEGG" id="xco:114145627"/>
    <dbReference type="CTD" id="55276"/>
    <dbReference type="GeneTree" id="ENSGT00940000156247"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008973">
        <property type="term" value="F:phosphopentomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05799">
        <property type="entry name" value="PGM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000017">
        <property type="entry name" value="glucose 1,6-bisphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000035">
        <property type="entry name" value="Pgm3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745">
        <property type="entry name" value="PHOSPHOMANNOMUTASE 45A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745:SF3">
        <property type="entry name" value="PHOSPHOPENTOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="53"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="12">
        <location>
            <begin position="226"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="13">
        <location>
            <begin position="342"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="611" mass="67806" checksum="36B40374C33B4652" modified="2018-12-05" version="1">MEDGLKPTGDTKLDQAIRQWLRYDRNPKTISLVQDMVKDGSMEALKKCFSSRMEFGTAGLRAAMGPGISCMNDLTIIQTTQGFCRYLEQSFGNLKERGVVIGYDARAHPTSGGSSKRFASLAAAVFISRGVPVHLFSEITPTPFVPFTVSHLGLCAGIMVTASHNPKQDNGYKVYWENGAQIVSPHDSGISKAIEENLEPWPASWNTEEALKATLLKDPFQDIKTQYFQAIQKHCHHREINKKSEVKIVHTSVHGVGHTFVQSAFKAFDLHPPYPVEEQKDPDPEFPTVKYPNPEEGKGVLTLSFALAEREGASVVLANDPDADRLAIAEKQESGQWRVFSGNELGALLGWWMFHCWSEQNSDAAAVKNLYMLASTVSSKILRAIALKEGFHFEETLTGFKWMGNRAKDLLDQGKSVLFAFEEAIGYMCCNSVLDKDGVSAAAIAGEMISYLATKSKSLSQQLTTIFEEYGYHISKNSYFICHDQEVIHNLFERLRNFGGKKDSYPSECGRFSISAVRDLTTGYDSNQPDKKAILSTSTSSQMITFTFSNGGVATMRTSGTEPKIKYYTELCAAPGNSDVTHLKKELDDLVDAIVENFFEPVKNKLQPKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M493</accession>
    <name>A0A3B5M493_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calmodulin binding transcription activator 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M493"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019210.1">
        <property type="protein sequence ID" value="ENSXCOP00000018973.1"/>
        <property type="gene ID" value="ENSXCOG00000014267.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167132"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="78" mass="8890" checksum="E06DD84D37D05509" modified="2018-12-05" version="1">MKPLLNILFCFALVVDEHGHLKIYLPKKLLECLPKCTSLPKERHRWNTNEGVCPAPYNLHHSRSPSSVSFHLSCLGSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5N4</accession>
    <name>A0A3B5M5N4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Glucosaminyl (N-acetyl) transferase family member 7</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M5N4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018685"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018920.1">
        <property type="protein sequence ID" value="ENSXCOP00000018685.1"/>
        <property type="gene ID" value="ENSXCOG00000014059.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160402"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008375">
        <property type="term" value="F:acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003406">
        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19297:SF178">
        <property type="entry name" value="BETA-1,3-GALACTOSYL-O-GLYCOSYL-GLYCOPROTEIN BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19297">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 14 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02485">
        <property type="entry name" value="Branch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="414" mass="47574" checksum="A921C4D4D50BF5EC" modified="2018-12-05" version="1">MHQLGGRKCSFLFCLGVVACSIIYWLSRTKLVGTPTSSRPFLTESKPTLAGIEKRENFCQVGSYIHSTNLQCSNLTKELHFINATLSHEEENYPLAFIVTIHKELDIFVRMLRAIYMPQNVYCIHVDAKAPNEYQETVQKLVSCFDNVFLSSRSEKVTYAGFSRLQADLNCMKDLSASPIAWRKVINLCGQDFPTMSNLELVQYMQSNEWRDKNMTPGVKQPATMKHRTRFQHHEIVGSHVVPRLPRHTKPPPPHNLKIYFGTAYYALTRDFVDFVLKNQIAHDFLEWSKDTFSPDEHYFVTLNHIKEAPGSRIDGGWEGAIRAIKWRDQEGKTHDGCKGHYARDICVYGIEDIPWIIEKKGMFANKFEINTFPEALDCLEQWHRNKVLNQATVPINPSWLIPSATGTYPRLGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6K6</accession>
    <name>A0A3B5M6K6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6K6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019070"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019307.1">
        <property type="protein sequence ID" value="ENSXCOP00000019070.1"/>
        <property type="gene ID" value="ENSXCOG00000014263.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159322"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048495">
        <property type="term" value="F:Roundabout binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050919">
        <property type="term" value="P:negative chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000080">
        <property type="entry name" value="Neurogenic locus notch 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000045">
        <property type="entry name" value="Slit guidance ligand 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000053">
        <property type="entry name" value="Slit guidance ligand 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000062">
        <property type="entry name" value="Slit guidance ligand 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000002">
        <property type="entry name" value="Slit guidance ligand 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000770">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051355">
        <property type="entry name" value="Notch/Slit_guidance"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45836">
        <property type="entry name" value="SLIT HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45836:SF9">
        <property type="entry name" value="SLIT HOMOLOG 3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01462">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 12" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017207806" evidence="13">
        <location>
            <begin position="20"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="560"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="598"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="638"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="683"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="14">
        <location>
            <begin position="722"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="931"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="586"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="626"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="664"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="709"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="935"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="956"/>
            <end position="965"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1102" mass="120475" checksum="133190B38BCC32A2" modified="2018-12-05" version="1">MIHMDQLIFLFLPVFSALSLQISRLSSSFRRSVFPPDSSFFIDFFPYSHSVHRDLSKNQISDVAADAFSGLRSLTSLVLYGNKIAELPKGIFDGLSSLQLLLLNANKINCLRVNAFQDLQNLNLLSLYDNKLQSISKGLFAPLRSIKTLHLAQNPFMCDCHLKWLADFLFDNPIETSGARCSHPRRLANKRISQVKGKKFRCTGQEDYRNRLSGECFQDLVCPERCRCEGTVVDCSNLKLTRIPPHIPEHTTDLRLNDNEISVLEAAGTFRKLPNLKKINLSNNKLRDLREGAFDGSAGVLELLLTGNKLTALQGRMFRGLSGLKTLWLMLTNSLTLLDDVMFRAVMADSANLLSNPYVCDCHLAWLGQWLKKTRVVSGNPRCHRPSFLKEIPIQDVATPDFTCDGGEENGCLPASGCPDVCTCSDGVVRCSNRGLHALPKGIPKDTTELYLEGNQLSSVPKELAALKQLSLVDLSNNSISTVAPFTFSNMTQLATLILSYNQIRCVPLHAFDGLRSLRLLTLHGNDLSTIPEGAFGHLTSLSHLCVCPPGFEGPRCEINPDDCDDNDCENNSTCIDGVNNYTCLCPPNYSGDLCDEVVDPCLTGFDPCQHDSKCVQVGRGYRCECPPGFVGQRCELDYDDCLENKCQHGAECVDAINGYTCVCKEGFSGLFCENPPPMILLQTSPCDQSECQNGGHCLVVAGEPICRCIPGYYGNKCDKISTVHFLGRDGFVELHGTKLRPTAHISLQVATDKDNGVLLYKDDLDPLALELYQGHIRLIYDITHYPPTTVYSVESVSDGLFHTVELLIQNRSLSLVVDNGAPKSLGKLGRQPSVDHNTQLYIGGVPPLVAASGLRPGPERPPPGFDGCIHKVRINGEMQDLTFVQGPAVLVGCHACSVCAAGACREGGQTGVTCSCPPGRMGALCDETAASDPCQNIRCVHGTCVATAQAYSCRCADGYQGPSCDRPACSCAHGECRTSESGVLVCHCEPGFTGPACNTELRCQGEMLREQLKRHQAMRTCTSTSRVPRVACPRSCQAAAPPGVCCGVTKTRRRKVAFRCTDGSSYSEDRRCLTSKCSLLIPEGGSYSGWKCFYSELDTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6Q3</accession>
    <name>A0A3B5M6Q3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6Q3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019382.1">
        <property type="protein sequence ID" value="ENSXCOP00000019145.1"/>
        <property type="gene ID" value="ENSXCOG00000014392.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000176879"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="110" mass="12268" checksum="09057FD5B45A7584" modified="2018-12-05" version="1">MHFCAVPLQACVEKIFGVLTSYASAVERISAFESCSEFTSTVNPALKKLHRDMGKCVRSKAEHQPVEHWKEAYVCQYTLERLFSFSILTARVFAAGDPAQHSEGSAHRCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7E2</accession>
    <name>A0A3B5M7E2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="32 33">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="32" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>(S)-lactoyl-CoA + L-lysyl-[protein] = N(6)-[(S)-lactoyl]-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61996"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:19466"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:231527"/>
            <dbReference type="ChEBI" id="CHEBI:231528"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:61997"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019417"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019655.1">
        <property type="protein sequence ID" value="ENSXCOP00000019417.1"/>
        <property type="gene ID" value="ENSXCOG00000014586.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155364"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002244">
        <property type="term" value="P:hematopoietic progenitor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05495">
        <property type="entry name" value="Bromo_cbp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20910">
        <property type="entry name" value="NCBD_CREBBP-p300_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15647">
        <property type="entry name" value="PHD_CBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15802">
        <property type="entry name" value="RING_CBP-p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02337">
        <property type="entry name" value="ZZ_CBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.20:FF:000001">
        <property type="entry name" value="E1A binding protein p300"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.20.1020.10:FF:000001">
        <property type="entry name" value="E1A binding protein p300"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="E1A binding protein p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.40:FF:000001">
        <property type="entry name" value="E1A binding protein p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000003">
        <property type="entry name" value="E1A binding protein p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000001">
        <property type="entry name" value="Histone acetyltransferase p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000034">
        <property type="entry name" value="Histone acetyltransferase p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.20">
        <property type="entry name" value="Coactivator CBP, KIX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1630.10">
        <property type="entry name" value="Nuclear receptor coactivator, CREB-bp-like, interlocking domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1020.10">
        <property type="entry name" value="TAZ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031162">
        <property type="entry name" value="CBP_P300_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013178">
        <property type="entry name" value="Histone_AcTrfase_Rtt109/CBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003101">
        <property type="entry name" value="KIX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036529">
        <property type="entry name" value="KIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009110">
        <property type="entry name" value="Nuc_rcpt_coact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014744">
        <property type="entry name" value="Nuc_rcpt_coact_CREBbp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037073">
        <property type="entry name" value="Nuc_rcpt_coact_CREBbp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056484">
        <property type="entry name" value="PHD_P300"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010303">
        <property type="entry name" value="RING_CBP-p300"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038547">
        <property type="entry name" value="RING_CBP-p300_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035898">
        <property type="entry name" value="TAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000197">
        <property type="entry name" value="Znf_TAZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13808">
        <property type="entry name" value="CBP/P300-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13808:SF34">
        <property type="entry name" value="CREB-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09030">
        <property type="entry name" value="Creb_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08214">
        <property type="entry name" value="HAT_KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02172">
        <property type="entry name" value="KIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23570">
        <property type="entry name" value="PHD_P300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06001">
        <property type="entry name" value="RING_CBP-p300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02135">
        <property type="entry name" value="zf-TAZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01250">
        <property type="entry name" value="KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00551">
        <property type="entry name" value="ZnF_TAZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF47370">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47040">
        <property type="entry name" value="Kix domain of CBP (creb binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69125">
        <property type="entry name" value="Nuclear receptor coactivator interlocking domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57933">
        <property type="entry name" value="TAZ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51727">
        <property type="entry name" value="CBP_P300_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50952">
        <property type="entry name" value="KIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50134">
        <property type="entry name" value="ZF_TAZ"/>
        <property type="match status" value="2"/>
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    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="20" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="16" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="17 22" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="26" id="KW-0732">Signal</keyword>
    <keyword evidence="18" id="KW-0804">Transcription</keyword>
    <keyword evidence="15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12 23" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 24" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017342063" description="histone acetyltransferase" evidence="26">
        <location>
            <begin position="17"/>
            <end position="2245"/>
        </location>
    </feature>
    <feature type="domain" description="TAZ-type" evidence="28">
        <location>
            <begin position="315"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="KIX" evidence="30">
        <location>
            <begin position="535"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="27">
        <location>
            <begin position="902"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="domain" description="CBP/p300-type HAT" evidence="31">
        <location>
            <begin position="1122"/>
            <end position="1499"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="29">
        <location>
            <begin position="1501"/>
            <end position="1549"/>
        </location>
    </feature>
    <feature type="domain" description="TAZ-type" evidence="28">
        <location>
            <begin position="1564"/>
            <end position="1645"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TAZ-type" evidence="23">
        <location>
            <begin position="315"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TAZ-type" evidence="23">
        <location>
            <begin position="1564"/>
            <end position="1645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="80"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="153"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="224"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="285"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="409"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="628"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="805"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1355"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1673"/>
            <end position="1735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1788"/>
            <end position="1822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1835"/>
            <end position="1866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1906"/>
            <end position="1933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1991"/>
            <end position="2022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="2077"/>
            <end position="2219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="80"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="110"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="224"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="250"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="285"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="410"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="633"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="835"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="867"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="1355"/>
            <end position="1367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="1368"/>
            <end position="1381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="25">
        <location>
            <begin position="1384"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="1715"/>
            <end position="1735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="1811"/>
            <end position="1822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="1837"/>
            <end position="1849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="1857"/>
            <end position="1866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="1914"/>
            <end position="1933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="1991"/>
            <end position="2004"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="2077"/>
            <end position="2102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="2117"/>
            <end position="2132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="2144"/>
            <end position="2156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS51727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="2245" mass="245914" checksum="F889FA1B67827D8F" modified="2018-12-05" version="1">MPYFSVFLSFCILVDLGSLPWDDLENDLPDELIPNGGELSQLSGLPSNGGALGEGAAIVPDAASKHKQLSELLRAGSTSSITGTGLNSASPQPGSMAPQLGTPLGKSPLAQGSPNSHSSPQGQKPGTPTGVVGQNSNNNAAAMGLSATGFSQAMINNSPGHSGLLGQGGQPQPGQVMNGGLVPSGGRGRGAGMAGLQCQGQTIPGSTAGPGASGSALVETLTQGGQQMGAHAAQQAGNMNKPYGQGAAGPGQQLNPQQQLQNKAVLASSLPPFPNELKGATVTSRPNMSPQQQAGMLPLANSGGVAVSSAGPTADPEKRKLIQQQLVLLLHAHKCQRREQANGEVRQCALPHCRTMKNVLNHMTHCQAGKSCQVAHCASSRQIISHWKNCTRHDCPVCLPLKNASDKRTQQPLLSSPNTSLQNPMSTVGMGQPSAPTINTSAPIDPSSMQRAYAALGLPYSSQAAGQAAAQQAPGQAAGAQNSQMALGGGAMGVATSEQTNLHTDSLSNTQLLSDGSAVGQMGSLPTAAPVSSASARKAWHEHVTQDLRNHLVHKLVQAIFPTPDPAALKDRRMENLVAYARKVEGDMYESANSRDEYYHFLAEKIYKIQKELEEKRRSRLQKQIISQAPIAGQGTQQPGLPQPNALGPRPQSEYWSHNMSAARGWVQWYIILPSLKVGMSPSRMPQAQGIMPGHSNMVGQTPSQGQFLPQTHFTPGSGAVSVSSAMNVTVGPGMGQPPAQAPVTQVRPSYFPVLHHLLCLFSSQLSQPGASLDNRVPTPASAASADLHSQHVGADLPAQDLKAETRTDQQEFEAAGGKIEPKTEAENDSASASVKKEEPEGKPEPMEVEEKKPEIKTEPKEEEENGTNGTTSSSPSQSRRKIFKPEELRQALMPTLESLYRQDPESLPFRQPVDPMLLGIPDYFDIVKNPIDLSTIKRKLDTGQYQEPWQYVDDVWLMFNNAWLYNRKTSRVYKYCSKLAEVFESEIDPVMQGLGYCCGRKYEFSPQTLCCYGKQLCTIPTGGTYYSYQNRYHFCEKCFNEIQGDSVTLGDDPAQPQTMISKDQFERRKNDVLDPEPFVECKDCGRKMHQICVLHYEVIWPSGFICDNCLKKSAKSRKENKFTAKRLQSTRLGMYIEDRVNKYLKRQNHPEAGEVFVRVVASSDKTVEVKPGMKARFVDTGEMPDTFPYRTKALFAFEEIDGVDVCFFGMHVQEYGSECPFPNTRRVYISYLDSIHFFRPRVLRTAVYHEILIGYLEYVKKLGYTQGHIWACPPSEGDDYIFHCHPPEQKIPKPKRLQEWYRKMLDKAFAERILHDYKDIFKQATEDRLTSANELPYFEGDFWPNVLEESIKELEQEEEERKKEETTAATETPEGTPSDSKNAKKKNNKKTNKNKSSVSRANKKKPGMPNVANDLSQKLYATMEKHKEVFFVIHLHSGPLANTLPPIVDPDPMISCDLMDGRDAFLTLARDKHWEFSSLRRCKWSTMCMLVELHNQGQDRFVYTCNECKHHVETRWHCTVCEDFDLCISCYNTKGHEHQMVKWGLGLDDDNNSQSGEASKSPQESRRLSIQRCIQSLVHACQCRNANCSLPSCQKMKRVVQHTKGCKRKTNGGCPVCKQLIALCCYHAKHCQENKCPVPFCLNIKHKLRQQQLQHRLQQAQLMRRRMATMQGRTMPLPSPPAAATPSTPTSHAQPTGPPQASVSQGKPGPHASPLHQQQSPLPQPPQQRQQQAPLAAIKMAHHIEMMAQAQQNQQNYRVNMNGLPMTPQPTQQQRMAQTMQMVAGPRGPQVMQQPSLGQWPAAAGPMPAQNQQPGVVPNQNTPQQAMSIQRAMMAPGQQGQQRMLMPQQPGPRPQTPQRTGTIPPNALQDLLRTLKSPSSPQQQQQVLNILKSNPQLMAAFIKQRTAKYHASQPQPQAGQPQQPQQQQPGMPAMQTIAMQGGPVQRAAMPPQQPQQAPVQGMAQLGSGQLMNAAHNANPQIQELYRRQLLRQQQQQQQQPQQQGVMPQGHLGQFPNQPQGTSVMYSHIRMQQQQQQQIAMQQAGAAGAGGVTMGQLPPMAQMAQPGMSMDSAQNLLHQRMQQQQQQQAQLPQQQQAVLKQQMGSPAHPSPMSPQTHLLAGQAQGAAHLSAQPGLANALGNQVRSPAPVQSPAQQQQPPPHSSPSRQVQNQPQPSPQHPALPHSGSPHPVLGGPLQSLEQGHLGTPEQSAMLPQLNTPNRGALSSDLGLVGDATGDTLERFVEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7X6</accession>
    <name>A0A3B5M7X6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7X6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019595"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019840.1">
        <property type="protein sequence ID" value="ENSXCOP00000019595.1"/>
        <property type="gene ID" value="ENSXCOG00000014721.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000178108"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="66" mass="6972" checksum="108DC05DD9ABA703" modified="2018-12-05" version="1">MRLRNVSVLTVLLFGLSGLVSLSWYTAFSSSRGKPGPGGGEEEEEEISSPGASSSPLEDEELVVVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M827</accession>
    <name>A0A3B5M827_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CAP-Gly domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M827"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017456"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017678.1">
        <property type="protein sequence ID" value="ENSXCOP00000017456.1"/>
        <property type="gene ID" value="ENSXCOG00000013167.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159557"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000044">
        <property type="entry name" value="CAP-Gly domain containing linker protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000005">
        <property type="entry name" value="CAP-Gly domain containing linker protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF77">
        <property type="entry name" value="CAP-GLY DOMAIN-CONTAINING LINKER PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="252"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="5">
        <location>
            <begin position="369"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="CAP-Gly" evidence="5">
        <location>
            <begin position="492"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="419"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="22"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="421"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="604" mass="67245" checksum="08939C3B0363C24E" modified="2018-12-05" version="1">MQEQEKERQDHEEQEEARVQREEDEELTEEEEDEEEEEYELEEERTEVENEEEQEEEQEEREEQEEQEEEEAPPAEPAAEPVPENQSPVQEPARRAMVHPSAQAPLPKDYAFTFFDPNDPACQEILADPRTSIPELFAIVRQWVPQVQHKIDVIGNEILKRGCHVNDRDGLTDMTLLHYSCKAGAHGVGDPAAALRLTSQLMSLGADVSLRSRWTNMNALHYAAYFDVPELIRVLLKAARPRVLNSTCSDFHYGTALHIAASNLCLSAVKCLLEHGANPTVRNDKGQVPAEVVPDPMDMSLDKAEAAMVAKDMKQLLLDAVPLSCNLPKATLPNYDNIPGNLMLAALGLKLGDRVVLDDLKTGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKLGIFAPVSKISKAVDPTPSSVTSTPRTPRMDLASRLAGRTKKEKKEKEKAQKKKTSVASLDPEGTNVEVGDQVLVAGQKLGIVRFYGKTDFAPGFWFGVELDQPTGKHDGSVFGVRYFSCLPKYGVFAPPSRVQRIGGLKEGSQNDGSMVKKVHQVSMSQPKRNFHTMRSPKDLTSESSISRLLFCCWFPWMLRAEMQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M961</accession>
    <name>A0A3B5M961_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DUF3504 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M961"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017816"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018040.1">
        <property type="protein sequence ID" value="ENSXCOP00000017816.1"/>
        <property type="gene ID" value="ENSXCOG00000013424.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166582"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057926">
        <property type="entry name" value="QRICH1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021893">
        <property type="entry name" value="ZMYM2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051284">
        <property type="entry name" value="ZnF_MYMT-QRICH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45736">
        <property type="entry name" value="ZINC FINGER MYM-TYPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45736:SF5">
        <property type="entry name" value="ZINC FINGER MYM-TYPE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12012">
        <property type="entry name" value="DUF3504"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25561">
        <property type="entry name" value="QRICH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="QRICH1-like" evidence="6">
        <location>
            <begin position="93"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="ZMYM2-like/QRICH1 C-terminal" evidence="5">
        <location>
            <begin position="211"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="44"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="409" mass="47492" checksum="24CA4AADA26330B6" modified="2025-10-08" version="2">MPVPMFLPVTMNSAERIVETIQEIKQKIPSDPYEAELILMAEMVAEGDEEKSSKTETRKDKTSAKESESKEAPAQDGETLSSAKKSVSAEILKLKSQYGIDAWKSWIQWRQMQPNLEQRPRPLELKEDLLRCTTAELSYGLCCFITEVKRPNGEPYPPDSLFYLCLGIQQYLFENGRVENIFMDRFYSKFSTDFTNMLKSFQPTVTPRGYVHSRVEEEFLWGCKQLGAYSPIVLLNTLLFFSCKYFGFTTVEQHRQLSFAHVMRCTKTNPDNSKTTFLRFYPPIPVNEAESDGVPAKRRKEEESKEEILEMMENTENPLRCPVRLYEFYLSKCSESVKQRTNMFYLHPERCCVPNSPLWFSSTPLDDNTMESMLIRILTVRELHLRTKKDQPVVSDPPFIPEEEDEDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9J9</accession>
    <name>A0A3B5M9J9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ran-specific GTPase-activating protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Ran-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Plays a role in RAN-dependent nucleocytoplasmic transport. Alleviates the TNPO1-dependent inhibition of RAN GTPase activity and mediates the dissociation of RAN from proteins involved in transport into the nucleus. Induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. Promotes the disassembly of the complex formed by RAN and importin beta. Promotes dissociation of RAN from a complex with KPNA2 and CSE1L. Required for normal mitotic spindle assembly and normal progress through mitosis via its effect on RAN. Does not increase the RAN GTPase activity by itself, but increases GTP hydrolysis mediated by RANGAP1. Inhibits RCC1-dependent exchange of RAN-bound GDP by GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with RAN (via C-terminus of GTP-bound form) but not with GDP-bound RAN. Identified in a complex composed of RAN, RANGAP1 and RANBP1. Identified in a complex that contains TNPO1, RAN and RANBP1. Identified in a complex that contains CSE1L, KPNA2, RAN and RANBP1. Identified in a complex with nucleotide-free RAN and RCC1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RANBP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9J9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020212"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020459.1">
        <property type="protein sequence ID" value="ENSXCOP00000020212.1"/>
        <property type="gene ID" value="ENSXCOG00000015152.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154389"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046604">
        <property type="term" value="P:positive regulation of mitotic centrosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13179">
        <property type="entry name" value="RanBD_RanBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000824">
        <property type="entry name" value="Ran-specific GTPase-activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045256">
        <property type="entry name" value="RanBP1_RanBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF94">
        <property type="entry name" value="RAN BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RanBD1" evidence="10">
        <location>
            <begin position="23"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="160"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="175"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00056716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="215" mass="24741" checksum="4B7DC88F85AA2F30" modified="2018-12-05" version="1">MADTKEDRETTGDGAEDTNHDPHFEPIVSLPEQDVKTLEEDEEVLFKMRAKLYRFASENDPPEWKERGTGDVKLLKHKEKGTIRLLMRRDRTLKICANHQISPLMELKPNSGSDRAWVWNTLADYTDESPKPELLAIRFLNAENAQKFKVKFDECKEEVKKQLEGSGNTDGANKVAEKLEELSVKDKAPEGKKEENKKETEKNEDEKKEVKAEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9S1</accession>
    <name>A0A3B5M9S1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Annexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="domain">
        <text evidence="6">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the annexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9S1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021003"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021261.1">
        <property type="protein sequence ID" value="ENSXCOP00000021003.1"/>
        <property type="gene ID" value="ENSXCOG00000015713.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155221"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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    <keyword evidence="3 6" id="KW-0106">Calcium</keyword>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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            <dbReference type="ARBA" id="ARBA00023216"/>
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        <source>
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    <sequence length="120" mass="13126" checksum="5A849952EF3D0995" modified="2018-12-05" version="1">QHTSCTPLCSDQSNKTTPPITCVAFYADAGVLEKAIKTKGVDEHTIIDVLVKRSNEQRQHIKEAYQQGYGKPLETELKKALKGDLEEVVLALLKTPAQYDAQQLKLAMKVGQTHGAGLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9U5</accession>
    <name>A0A3B5M9U5_9TELE</name>
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        <submittedName>
            <fullName evidence="10">Leucine rich repeat transmembrane neuronal 4 like 2</fullName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Atherinomorphae</taxon>
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            <taxon>Poeciliidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
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        <property type="entry name" value="LEURICHRPT"/>
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        <property type="entry name" value="LRR_TYP"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
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    <keyword evidence="6" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="421"/>
            <end position="443"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="552" mass="62729" checksum="3F8B7BC899B4A724" modified="2018-12-05" version="1">MHFQKLSHPFDCLCPTLPPTGSVMLDWRLSCLLLQAAALLLLSKGERMCPASCRCEGKIVYCESGIFQDIPINITTGCQGLSLRYNNLLVLLPYQFAQLNQLIWLYLDHNSIHSIDALAFHGVRRLKELILSSNKLSFLHNNTFSAIPNLRNLDLSYNQLQSLQPGYFYGLRKLQNLHLRSNGLKQILIRTFLECRSLEFLDLGYNRLHSLTRTTFLGLFKLRELHLEHNQFSRINFYIFPRLTNLQALYLAWNRIRSISQGVPWTWPKLQKLDLSGNEIQTLEPDVFRCMPSLQTLNLETNKLSSVPVEVVEAWTALTTISLAGNAWDCSPSICPLMGWLRTFRDPKDINMICSGPKATQGERVVDVVRNHSICADAANIFSTTTMADPSTLSSMPPFSPQTSTPFIPELQFEHMAFHKIIAGSVALFLSVALILLVIYVSWRRYPNTMRQLQEHSVNHKRRKKARKQEQDLNSQLQEYYLTYHSNSEALDSLVNENRPCTCTISGSIECEQEGRGFESRPGVSLRGVCMFSLCMRGFSTGNPASSQSPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAE4</accession>
    <name>A0A3B5MAE4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">45 kDa calcium-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Stromal cell-derived factor 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
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            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Golgi apparatus lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CREC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAE4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020552"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020802.1">
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        <property type="gene ID" value="ENSXCOG00000015399.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01010000222360"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005796">
        <property type="term" value="C:Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:calcium-ion regulated exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd16225">
        <property type="entry name" value="EFh_CREC_cab45"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="45 kDa calcium-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000207">
        <property type="entry name" value="Putative 45 kDa calcium-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CAB45_EFh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827:SF98">
        <property type="entry name" value="45 KDA CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10827">
        <property type="entry name" value="RETICULOCALBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="129"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="168"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="269"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="309"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="393" mass="46500" checksum="A39B9E7FC224CB58" modified="2018-12-05" version="1">MSRDDSIFFSKNCIYFLFNFSKLIWLSFCLHVFPEAIMALCRDLWSRNLLALSLLFYFLLTLDVHARPANMSSSKDKSPATKDENEILPPDHLNGLKMEMDGHLNKDFHQEVFLGKEMDDFEEDSEPKQNRKKLIEIFTKVDVNKDRSVSAKEMQHWIVEKTKEHFKEAMEENKNSFRAVDPDGDGYVTWNEYKVKFLASKGFNETEVTDKIKKKEELKLDEETQEVLESLKDRWFQADNPPADQRLDEEEFLSFLHPEHSKGMLRYMVKEIVRDLDQDRDWKLTLSEFISLPVGTVENQQGQDIDDDWVKERKKEFEEVIDSDRDGIVTMNELEEYMDPMNEHNALNEAKQMIAVADENQNHRLELEEILRYSEYFTGSKLMDYARNVHEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAN9</accession>
    <name>A0A3B5MAN9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAN9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016774"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016991.1">
        <property type="protein sequence ID" value="ENSXCOP00000016774.1"/>
        <property type="gene ID" value="ENSXCOG00000012653.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160230"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007249">
        <property type="term" value="P:canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038061">
        <property type="term" value="P:non-canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034097">
        <property type="term" value="P:response to cytokine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000451">
        <property type="entry name" value="NFkB/Dor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030492">
        <property type="entry name" value="RHD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169">
        <property type="entry name" value="NUCLEAR FACTOR NF-KAPPA-B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169:SF18">
        <property type="entry name" value="TRANSCRIPTION FACTOR RELB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01204">
        <property type="entry name" value="REL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RHD" evidence="2">
        <location>
            <begin position="15"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="59" mass="6514" checksum="708583E7E057CD53" modified="2018-12-05" version="1">LDEINPSILKPKCLLVVVEEPKDRGMRFRYECEGRSAGSILGASSTDNNKTQPTIEVHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBB7</accession>
    <name>A0A3B5MBB7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein KRI1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
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    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KRI1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBB7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020865"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021121.1">
        <property type="protein sequence ID" value="ENSXCOP00000020865.1"/>
        <property type="gene ID" value="ENSXCOG00000015624.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005605"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018034">
        <property type="entry name" value="Kri1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024626">
        <property type="entry name" value="Kri1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490:SF5">
        <property type="entry name" value="PROTEIN KRI1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490">
        <property type="entry name" value="ZINC FINGER, ZZ TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05178">
        <property type="entry name" value="Kri1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12936">
        <property type="entry name" value="Kri1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kri1-like C-terminal" evidence="4">
        <location>
            <begin position="371"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="38"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="125"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="175"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="269"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="476"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
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        <location>
            <begin position="183"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="275"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="310"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="327"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="541"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="553"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF12936"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="614" mass="73418" checksum="AC634625D0F2A8A1" modified="2018-12-05" version="1">KYEDDDDSDEEEAAKRRERAASPSYIQEQKELKEFRKFVEDSDEEEEEEEGEASKLLTRRIKTQEEKDKEEADYVDWLKGQAELDGPEEVKDMQKYLREYWNDPQLDDKERFLRDYVLNKGYLDEEEDIPTYDELVQDDVDDSEEEGESFLERQEDFERSYNFRYEEPLQIKTYPRNIATSVRTKDDRRKQKREEVKERKLKQEKEQKQEQLKQLKNLKRKEIMDKLQQLQELTGNEQLAFSQADLDGDFDPNQHDRLMQKFFGDEYYGQEEEEKPQFDDEHWNWDTWTGEEREEGHDEAEYSASGPHCDDEDFIMDADYDPSQHTASKKKKKKEKKKLKKDDLPQMGKKKKKSHFAEVVTRTKPVFDPEKTFEQYLDEYYKLDYEDIIDDLPCRFRYRQVVPNDFGLTTDEILSANDQELNHWCSLRRTCMFSEREEMSDLKNYKAKAQNVRKKKEVLSSVYSEDKETLEVKSKLGKKRRDRLRNTEKQSEEADEAVQALSEAVDGTEEGDEILVPKKKKQKSKLEEQTQDTVEQVADEQNGRENRTERPAWAKKPRRSGGRLKSGIKGVKIGGREFSRHRLKAYGLNPRRLYFRQLGRHKRKEQEKKLKKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBG9</accession>
    <name>A0A3B5MBG9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G2 and S phase-expressed protein 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBG9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020920"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021177.1">
        <property type="protein sequence ID" value="ENSXCOP00000020920.1"/>
        <property type="gene ID" value="ENSXCOG00000015662.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154189"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026657">
        <property type="entry name" value="DDA3/GTSE-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032768">
        <property type="entry name" value="GTSE1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21584">
        <property type="entry name" value="DIFFERENTIAL DISPLAY AND ACTIVATED BY P53 DDA3 /G2 S PHASE EXPRESSED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21584:SF10">
        <property type="entry name" value="G2 AND S PHASE-EXPRESSED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15259">
        <property type="entry name" value="GTSE1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G2 and S phase-expressed protein 1 N-terminal" evidence="6">
        <location>
            <begin position="11"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="190"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="225"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF15259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="323" mass="35000" checksum="9B1101E5BC3CED19" modified="2018-12-05" version="1">MRFPSLFSPCNEKFDFDVSLSKGEEEDDDEVFVGHTEQCVSTDVSSQPEEGGGDVQAVWTPLTADQLEAVCEEAHRLASQLQCREPHVQNTNTTHTASDVDMNSMATTEEFIQDTEVKLGLLDHTASSLSPIKRQTFCVQDSPLQQLPPAVQHHLLRGSSSSRPAARLVTSSPMVGARLQARPALLGKTANSTLHAPSGPAPSSVRREPSLSRPRRSTLSASAEPLSSRTGFRSQPTQAKKPPDAEQAKAARSTPLKRAESTPVQPTPPKRTGSVGATCSVRLQSGVKTRSKPRGLVHPTSNMADNAEGENIRWAFRLMFCQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDM7</accession>
    <name>A0A3B5MDM7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">pantothenate kinase</fullName>
            <ecNumber evidence="5">2.7.1.33</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantothenate + ATP = (R)-4'-phosphopantothenate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16373"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the type II pantothenate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.33" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDM7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017804"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018028.1">
        <property type="protein sequence ID" value="ENSXCOP00000017804.1"/>
        <property type="gene ID" value="ENSXCOG00000013415.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157373"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.510:FF:000001">
        <property type="entry name" value="pantothenate kinase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004567">
        <property type="entry name" value="Type_II_PanK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280">
        <property type="entry name" value="PANTOTHENATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12280:SF23">
        <property type="entry name" value="PANTOTHENATE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03630">
        <property type="entry name" value="Fumble"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="197" mass="22369" checksum="5D3FCC649543E131" modified="2018-12-05" version="1">MDIGGTLVKLVYFEPVDITAEEEQEEVENLKSIRRYLTSNVAYGKTGVRDVHLELRNLTMCGRTGNLHFIRFPTQAMPRFIQMGRDKNFSSLHTTLCATGGGAYKFENDFRTMADLELLKLDELDCLIRGLLFIDRVGFNGHPECYYFQNPSDPQSCVKKPCNLDNHFPMLLVNIGSGVSILAVYSENNYKRVTGTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MED3</accession>
    <name>A0A3B5MED3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LRRNT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MED3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019601"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019846.1">
        <property type="protein sequence ID" value="ENSXCOP00000019601.1"/>
        <property type="gene ID" value="ENSXCOG00000014724.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162953"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF196">
        <property type="entry name" value="RETICULON 4 RECEPTOR LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01462">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045034689" description="LRRNT domain-containing protein" evidence="5">
        <location>
            <begin position="29"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="6">
        <location>
            <begin position="28"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="133" mass="14871" checksum="792C9A8D6B04CFB9" modified="2025-10-08" version="2">MQRVKRRAGLRVWAPLLAALLWAAAAHGCPHKCSCSGSHVDCQGLGLKTVPKGVPRSAERLDLNRNNITRITKVDFSGLKNLRILRLNRNKLQFLPELLFQSNPKLGRVDLSENQIQAVPRKAFRGITNVKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEX4</accession>
    <name>A0A3B5MEX4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Neural cell adhesion molecule L1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Growth cone</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEX4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018179"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018410.1">
        <property type="protein sequence ID" value="ENSXCOP00000018179.1"/>
        <property type="gene ID" value="ENSXCOG00000013334.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157024"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:growth cone"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:axon guidance"/>
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        <property type="term" value="P:brain development"/>
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        <property type="entry name" value="neural cell adhesion molecule L1"/>
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        <property type="match status" value="10"/>
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        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="Neurofascin/L1/NrCAM_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF12">
        <property type="entry name" value="NEUROFASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Bravo_FIGEY"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="5"/>
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    <keyword evidence="7" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
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        <location>
            <begin position="20"/>
            <end position="1134"/>
        </location>
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            <begin position="40"/>
            <end position="132"/>
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            <begin position="136"/>
            <end position="215"/>
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            <begin position="428"/>
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            <begin position="531"/>
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            <begin position="610"/>
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            <dbReference type="ARBA" id="ARBA00004251"/>
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    <sequence length="1134" mass="128255" checksum="DFD8582FF79C1BF5" modified="2018-12-05" version="1">MRTLVGPGLLLFLWQSIWALDVPLEKMPDLSLILSARQPPTIIKQSLQEHIVDPKETMVMECEAKGNPHPIFSWRRNGKYFNVARDSQVSMRRRSGTLDIYAKHNPEQYEAEYQCVASNLYGSAYTHKIRLRLYRPPVWPREILEPVIISAGLPLVLPCDAPPGPPKPETYWMSSSFPTMQAVRQDRRVSMGVNGDLYFSNVLVNDSTTDYCCSARFPYKNMIQQKMPVVVKVLTMAEAAPTWLSPKGSSSSILVLQGEELLLECIAGGMPTPRITWTKDGEDLPVTSSMKVKNFNKMIQIPKASFEDAGEYMCAATNRIGYIEHTITVRVRAAPFWLERPTNLILAPEENGQLVCRSDGAPRPTISWSINGEPIETSTPQSNRQVSGETITFRSVSTENTAVYQCNASNEHGYLLANAFVNVLHATPRIIGPRNELIKTIEGHRAFLDCRFFGSPVPELYGQGNLEGNRFRIHRNGTLEIKRIQIEDQGTYLCVISNIAGRDENQVRVEVKGKDDTENVCHQESMKVLRGSDVRLECGVKPDTAASITTAWMKNKKQAALSWRVSMDGSNLVITNVNRGDEGNYTCVIQNDLEQKSASAHLMVMDRPSPPTDLELSDPYERSVRLSWVPGDSNHSPINVQYDDDDWIPGKWKNLSTYPGSLNSVMLHLSPFTYYEFRVIANNEIGSSRPSRPSKRFQTSAPDVIPKNLKGVGTWRNNMEISWEPLTWREWNGPHLKYLVWWRRRDSREEWKNATTKWLKYYIYDADTFTPYEIKVQAVNDFGLGPESPVVTGYSGEDRPIAAPLNLRVSDIESTQVTLHWDPVTRESIMGELKEYKVYYWRDSSQLRWHRVNRGMRSKVFPNSDPEPSGVVTDLIPYSNYKMYIVVTNSRYEGPPSNHIHFSTPEGVPSAPKSFRIQQRHLDSIYVDWDLPEEPNGVITGYSLKYQTGMPKDSNKRCQAHLEIRKNQLLSNSRKAGYGSLPLITIAVSSPHYTTEIYAQDQVDISTQGWFIGIMCAVALIVLILLIVCFIKRSRGGKYPGTNEGKSCEIFQYAEVHTGKTYSKPRAQPSVAAILKRSDSDDSLVEYGEGGEIQFNEDGSFIGQYTGTRRDVRDLDFGGNLELHSPMNTIYSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFF1</accession>
    <name>A0A3B5MFF1_9TELE</name>
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        <recommendedName>
            <fullName evidence="1">DUF4939 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSXCOG00000016673.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="entry name" value="DUF4939"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16297">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4939" evidence="1">
        <location>
            <begin position="35"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="97" mass="11435" checksum="47797187FE2558C0" modified="2025-10-08" version="2">MHEVLLTNYSQLSRRIYPRWRSIKLFFFIFSFSPQSFQTDAAKIYYVIGLLRGQALKWAEAKLGQQVLFQGTYDSFVSAFKQTFGYTESHSVTDFFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGV5</accession>
    <name>A0A3B5MGV5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="3">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGV5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020594.1">
        <property type="protein sequence ID" value="ENSXCOP00000020346.1"/>
        <property type="gene ID" value="ENSXCOG00000015249.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159562"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06624">
        <property type="entry name" value="STKc_ASK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000054">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000067">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043969">
        <property type="entry name" value="MAP3K_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584:SF363">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19039">
        <property type="entry name" value="ASK_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="100"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="414" mass="46427" checksum="87D80DC6EB0F28F3" modified="2018-12-05" version="1">TNLIPSPICFFPTLSLFFLYFPTEITFSVRSVCSISKFDERCCFLYVHDNSDDFQIYFSTEEQFCSLVKEMISDGTGNAVELEGEGDGDTLEYEYDTDENGDRVVLGRGTYGVVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKYLKHRNIVQYLGSVSENRYIKIFMEQVPGGSLSALLRSKWGPLKEATIIFYTRQILEGLRYLHENQIVHRDIKGDNVLVNTYSGVLKISDFGTSKRLAGVNPCTETFTGTLQYMAPEIIDKGPRGYGAPADIWSLGCTIIEMATGKPPFHELGEPQAAMFKVGMFKIHPEIPESLSLEAKSFILRCFEPDPNKRPIAADLLRDIFLRQTTKGKKSKIAFKPSGGRQINTLRSAATSTTLMLGSSVASETFPDPHRNPPAPPFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGW0</accession>
    <name>A0A3B5MGW0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Early endosome membrane</location>
            <topology evidence="12">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027897628.1">
        <property type="nucleotide sequence ID" value="XM_028041827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGW0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022982"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023259.1">
        <property type="protein sequence ID" value="ENSXCOP00000022982.1"/>
        <property type="gene ID" value="ENSXCOG00000016788.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114159736"/>
    <dbReference type="KEGG" id="xco:114159736"/>
    <dbReference type="CTD" id="5878"/>
    <dbReference type="GeneTree" id="ENSGT00940000154971"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120084">
        <property type="term" value="P:endothelial tip cell filopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001702">
        <property type="term" value="P:gastrulation with mouth forming second"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071425">
        <property type="term" value="P:hematopoietic stem cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090049">
        <property type="term" value="P:regulation of cell migration involved in sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030100">
        <property type="term" value="P:regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008593">
        <property type="term" value="P:regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030947">
        <property type="term" value="P:regulation of vascular endothelial growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000180">
        <property type="entry name" value="Member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="185"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="220" mass="23589" checksum="205554158E83701F" modified="2018-12-05" version="1">MAGRGGPARTNGTAASNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVFDITNTDTFTRAKNWVKELQRQASPNIVIALAGNKADLASKRAVDFQEAQAYADDNSLLFMETSAKTAMNVNEIFMAIAKKLPKNEPQGGAGQGGRARGGVDLQETAPQGRSGQCCGGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MH28</accession>
    <name>A0A3B5MH28_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Integral membrane protein GPR175</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0359 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MH28"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023618"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023901.1">
        <property type="protein sequence ID" value="ENSXCOP00000023618.1"/>
        <property type="gene ID" value="ENSXCOG00000017645.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016807"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018781">
        <property type="entry name" value="TPRA1/CAND2/CAND8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15876">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN ADIPOCYTE-ASSOCIATED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15876:SF8">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN ADIPOCYTE-ASSOCIATED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10160">
        <property type="entry name" value="Tmemb_40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017273521" description="Integral membrane protein GPR175" evidence="8">
        <location>
            <begin position="21"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="389" mass="42817" checksum="7D74D4E98AB50EB0" modified="2018-12-05" version="1">MFFSFFPLLGTDNMLATVTAVVTLAQYNDTVSPTPLENTSVFPTEGQANITKPHKCLQVLYEDIGDSRVRFWDILLLVPNVAFFIFLMWKLPSARAKIRLTSSPIFVTFYLLVFVVAAVGITRAIVSMTVSASSAATIVDKVLWEITRFFLLAIELSVVILGLAFGHLESKSSIKRVLAITAVLALAYSITQGTLEILYPDSHLSAEDFNIYGHGGRHFWLASSCFFFLVYSLIVVLPKTPVRERISLPSKRSFYVYAAILSLLNFVQGLGSALLCAGIIEGLWLVFSPLLHVNILSLFFSEPKILFSYKSQVDEPDESDVHLPPTIAATLGRKDFTDQGLYYSSTQIDGAGPGSSRVVGAYLDDVASGPYGSNSINSIEADRWRPVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHL6</accession>
    <name>A0A3B5MHL6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the large ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHL6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023247"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023525.1">
        <property type="protein sequence ID" value="ENSXCOP00000023247.1"/>
        <property type="gene ID" value="ENSXCOG00000017362.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012628"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01417">
        <property type="entry name" value="Ribosomal_L19e_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.240:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1650.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01475">
        <property type="entry name" value="Ribosomal_eL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_eL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023638">
        <property type="entry name" value="Ribosomal_eL19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_eL19_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033935">
        <property type="entry name" value="Ribosomal_eL19_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057259">
        <property type="entry name" value="Ribosomal_L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057260">
        <property type="entry name" value="Ribosomal_L19e_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006343">
        <property type="entry name" value="PRK08570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25476">
        <property type="entry name" value="Ribosomal_L19e_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="Ribosomal protein L19 (L19e)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00526">
        <property type="entry name" value="RIBOSOMAL_L19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL19" evidence="8">
        <location>
            <begin position="3"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="157"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="159"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="196" mass="23490" checksum="C667DDC1F7F311A1" modified="2018-12-05" version="1">FSMLRLQKRLASSVLRCGKKKVWLDPNETNEIANANSRQQIRKLVKDGLIIRKPVTVHSRARCRKNTLARRKGRHMGTGKRKGTANARMPEKLCWMRRMRILRRLLRRYREAKKIDRHMYHSLYLRAKGNVFKNKRILMEHIHKLKADKARKKLLADQAEARRTKTKEARKRREERLQAKKEEFIKSLSKEEETKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIP7</accession>
    <name>A0A3B5MIP7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RAB5B, member RAS oncogene family</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027878674.1">
        <property type="nucleotide sequence ID" value="XM_028022873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIP7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024208"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024499.1">
        <property type="protein sequence ID" value="ENSXCOP00000024208.1"/>
        <property type="gene ID" value="ENSXCOG00000018079.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114147998"/>
    <dbReference type="CTD" id="5869"/>
    <dbReference type="GeneTree" id="ENSGT00940000166096"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000995">
        <property type="entry name" value="RAB5B, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="185"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="216" mass="23770" checksum="56149D523F386C68" modified="2018-12-05" version="1">MSSRGSGSRSNGSLPQTKICQFKLVLLGDMAVGKSSLVLRFVKGQFDEFQETTIGAAFLAQSVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVFDITKPETFERAKAWVKELQRQASPNIVIALAGNKADLAEKRLVEYEEAQTYAEDTGLLFMETSAKTAMNVNELFLAIAKKMPKTDTQNPTHAARHRGVNLQDPDAHSTRACCGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MK25</accession>
    <name>A0A3B5MK25_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Inhibitor of nuclear factor kappa B kinase subunit epsilon</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MK25"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024704"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025003.1">
        <property type="protein sequence ID" value="ENSXCOP00000024704.1"/>
        <property type="gene ID" value="ENSXCOG00000018454.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182937"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045089">
        <property type="term" value="P:positive regulation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000100">
        <property type="entry name" value="inhibitor of nuclear factor kappa-B kinase subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000106">
        <property type="entry name" value="serine/threonine-protein kinase TBK1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.420">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051180">
        <property type="entry name" value="IKK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041309">
        <property type="entry name" value="TBK1_CC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041087">
        <property type="entry name" value="TBK1_ULD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22969">
        <property type="entry name" value="IKB KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22969:SF10">
        <property type="entry name" value="INHIBITOR OF NUCLEAR FACTOR KAPPA-B KINASE SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18394">
        <property type="entry name" value="TBK1_CCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18396">
        <property type="entry name" value="TBK1_ULD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="674"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="13"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="738" mass="84566" checksum="AD2D16BFB6FCD6D3" modified="2018-12-05" version="1">MSGITGSTPNYLWSVHDVLGQGATASVYKARNKVRSGELVAVKVFNVLSYSRPHDVQMREFEMLKKLNHSNIVRLFAVEELQTKQKVLVMEYCSGGSLLTLLEDPENAFGLPEAEYLTVLQCVVQGMNHLREYGVVHRDIKPGNIMRQVGEDGKSVYKLTDFGAARELEDDEKFMSIYGTEEYLHPDMYERAVLRKPHQKSYGVSVDLWSIGVTLYHSATGSLPFTPFEGPRKNKLTMYKITTEKPAGAIAGTQRVEGGPIEWSYHLPYSCQLSQGLKALLVPVLAGIMEADQGRCWGFDQFFTATTDVLQRQPVYVFSLQQAMAHSIYRGKFLAFHKILPKIMSVFFEEVAAQTGIGIPQQQLMYLGHDLVLEGSMKVVNLPCTSSSQPLILLSRMPEINTSLPFREPETPPVPFKFDVVTDYSFSKIIVGIVHQYKRIVLLLHTYRELLLQGYYSYMMKLRHECTEAMHSIAIITIRLQSCLTLYSDVLLLSSQVHQHLPIYSTGIREFQTQLQHLQIQQAKLAETLTHDKGCQKMQMLLEKITAIHQQYRKDRLTGKLLYNDEQIHKFEKMHLVSHIKRVKSLFREECVQRYKELLASTRTWSSVLLDMQTRLQDFTSFSAGLLGDLDISEQQQNKVNMSFFMTFRFFCSIRSSAFSLWEGQTFLFIYEHVLLYFGFFMGFFFTLGMFGISEDVSSRASLPQAMNRILSSSQFGRAEQQPEITPRDKSQMVFRWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLN8</accession>
    <name>A0A3B5MLN8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Probable phosphatase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CTDSPL2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLN8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022056"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022324.1">
        <property type="protein sequence ID" value="ENSXCOP00000022056.1"/>
        <property type="gene ID" value="ENSXCOG00000016480.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000240503"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000015">
        <property type="entry name" value="CTD small phosphatase-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="6">
        <location>
            <begin position="211"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="394" mass="45007" checksum="E90849394CAF9137" modified="2018-12-05" version="1">MRLRVRKAPQHPSKGHRTLPYQAKRKHSEVEPSSPVSGRGKMQKNESGLLSTIKKFIRGNAVKVEQEIPAKRSRIDCNSDNDLITSTPQGLSNKSASRVCRKTQNTMTCNSKPIKPNGKLEAPIDSPSSPPRTTLLGTIFSPVFNFFSPATKTAPGCMPDGSYPHSLPSAPAETSYEEDWEVFDPYFFIKHVPPLTEEQLTRKPALPLKTRSTPEFSLVLDLDETLVHCSLNELEDAALTFPVLFQDVIYQVYVRLRPFFREFLERMSQLYEIILFTASKKVYADKLLNILDPKKQLVRHRLFREHCVCVQGNYIKDLNILGRDLAKTVIIDNSPQAFAYQLSNGIPIESWFVDRNDNELLKLVPFLEKLVELNEDVRPHIRERFRLHDLLPPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MMN8</accession>
    <name>A0A3B5MMN8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MMN8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025358.1">
        <property type="protein sequence ID" value="ENSXCOP00000025053.1"/>
        <property type="gene ID" value="ENSXCOG00000018703.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286969"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="45"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="264" mass="28970" checksum="7A1F64425D09DF8F" modified="2018-12-05" version="1">TQRLKMQRWFLHSNCDGEQTLSTKTKIGGPVGNSSSVEQILVPIMDSLILILGVTGHSLVMVSTMGTGTDILLLALSAADLLLLSTIPFYTVSIAMQKWPFGNVMCRLVGFLGSACSSASVFTLAALAVSRYLTVVKPTRAYSLLIPRRVSMIAVLIWVPACSLGAPQLAFRSVGPPREITDGLTCFAFLSHEQQMIYGLFHFLVAFMLPLVTIAVAYGSIYVFLWRGQQAGRAPQVERYQSKNIKHNIKLPLLTNYPKQTNKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MN52</accession>
    <name>A0A3B5MN52_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MN52"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021146.1">
        <property type="protein sequence ID" value="ENSXCOP00000020889.1"/>
        <property type="gene ID" value="ENSXCOG00000015638.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286959"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818:SF157">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
        <property type="entry name" value="ZINC FINGER PROTEIN ZIC AND GLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="206"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="234"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="262"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="290"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="29"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="356" mass="40419" checksum="743E9CBB7545D5C9" modified="2025-10-08" version="2">MAFLVLTDEEHLVLKQETDDILVIPLNRDKEQKQKEKYEKTKLQKGQTESSKQKTDKKAHPQQKLYSCKICDEAFSQSSLLTTHMTTHTDKNPFTCSTCGKSFTCRSSLLGHMMIHTGEKPFSCVTCGKSFQGKSNLTRHTIIHTGVKPFLCRTCGKCFHYKSNLTSHIRTHTGEKPFSCGTCGKSFYQKKYLTSHNRIHTHEKPFSCRSCGKGFCQKTGLISHMRTHTGEKPFLCGTCGKSFHHNSSLVTHMWIHTDKKSFLCGSCGKNFHQQSSLTCHMRIHADENPFSCRTCGKSFHHKRSLTRHMKTHTGEKSFSCRTCGKGFFQKSNLTSHMSIHTSEKSQHLSNIHSSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNN2</accession>
    <name>A0A3B5MNN2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Transcription initiation factor TFIID 150 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNN2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022771"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023047.1">
        <property type="protein sequence ID" value="ENSXCOP00000022771.1"/>
        <property type="gene ID" value="ENSXCOG00000017014.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000420"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09839">
        <property type="entry name" value="M1_like_TAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000003">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057345">
        <property type="entry name" value="Ig-like_TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037813">
        <property type="entry name" value="TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057991">
        <property type="entry name" value="TPR_TAF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25316">
        <property type="entry name" value="TAF2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25577">
        <property type="entry name" value="TPR_TAF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 Ig-like" evidence="9">
        <location>
            <begin position="465"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 TPR repeats" evidence="10">
        <location>
            <begin position="585"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="970"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1056"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1066"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1094"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1116"/>
            <end position="1135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1135" mass="130611" checksum="4CF8FD3BA78A1BAD" modified="2018-12-05" version="1">INFQRKSVIGYVELTIFPTVVNLNRIKLNSKQCRIYRVRVNDLEAPFIYNDPTLEVCHNESKQRNLNYFSSAYTAAVSAVDPDAGNGELVIKVPSELWKQGDELKVLKVYIEFSLDQPKGGLHFVVPDVEGSMAERGAHVFSFGYQNSTRFWFPCVDSYSELCTWKLEFTVDASMVAVSCGDLVETIYTHDMRKKTFHYVLPIPTAAPNISLAVGPFEILVDPYMHEVTHFCLPQLLPLLKHSMSYLHEIFEFYEEILTCRYPYSCFKTVFVDEAYVQVSSYASMSIFRFLLDRRPVTLWKGMADEWVLKGISGYIYGLYLKKTFGVNEYRHWIKEELDRIVEYELKMGGVLLHPTFSGGKEKDNPTPHLHFSIKHPHTLSWEYFKMFQCKAHLVMRLIENRISMEFMLQVFNKLLSLASTASSQKYQSHMWSQMLLSTHAFLKSISNVSGKDIGPLIKQWVDQSGVVKFFGSFAFNRKRNVLELEIRQDYTSSGTQKYVGPIKVTVQELDGSFNHTLQIEENSLKHDIPCHSKSRRNKKKKIPLMNGEEVDMDLSAMDADSPLLWIRIDPDMSILRKVEFEQADFMWQYQLRYERDVVAQEEAITALEKFPTPASRLALTDILEQEQCFYKVRMQACFCLAKIANSMVSTWQGPPAMKSLFTRMFCCKSCPNIVRTNNFINFQSYFLQKTMPVAMALLRDVQNLCPKDVLNFILDLIKYNDNRKNKFSDNYYRADLIEALTNSLTPAISINNEVRTVDNLNPDVRLILEEITRFLNMEKLLPSFRNTITVSCLRAIRQLQKNGHIPSDPSLFKAYAEYGHFVDVRVSALEALVDYTRVERSSADLQWLLDMVQSDPVHYVRHEILGMLCKNPPFTKSADSGLCNEALVDQLWKLMNSGTAHDWRLRCDAVNLYYTLFGLTRPSCLPLPELGLVLNLKEKKAVLNPTIKPELGVSVVMDTPASIGIHGRKAETPLGSPPEPGQILQHQEDDGRAGRYKIRFNTMEEEDIDMETVHDSQAFIHHQLNLLERPSTPGRSSLLSTPATLAPPFSKETASLSFKHSGDHAHHHHHHHHEHKKKKKKHKHKHKHKHKHESKDKEKEKDRDRDRDRDRDNSYAYSNSPASGRSLRSPSLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPT3</accession>
    <name>A0A3B5MPT3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">F-box/WD repeat-containing protein 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MPT3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021489"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021752.1">
        <property type="protein sequence ID" value="ENSXCOP00000021489.1"/>
        <property type="gene ID" value="ENSXCOG00000016087.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154986"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031642">
        <property type="term" value="P:negative regulation of myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001205">
        <property type="term" value="P:negative regulation of osteoclast development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903378">
        <property type="term" value="P:positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901800">
        <property type="term" value="P:positive regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000060">
        <property type="term" value="P:positive regulation of ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042752">
        <property type="term" value="P:regulation of circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032006">
        <property type="term" value="P:regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010992">
        <property type="term" value="P:ubiquitin recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22133">
        <property type="entry name" value="F-box_FBXW7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000004">
        <property type="entry name" value="F-box/WD repeat-containing protein 7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000032">
        <property type="entry name" value="F-box/WD repeat-containing protein 7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF1">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="4 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="11">
        <location>
            <begin position="126"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="239"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="265"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="305"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="345"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="385"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="425"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="468"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="555" mass="62331" checksum="EEA0B672BE0EC79C" modified="2018-12-05" version="1">MFSNVLPLFPSFSPQMKRKLDHGPEVRSFPSGKKPCKGPEYPSPTGIVPCPATPTTFGHIRAANGQGQQRRRITSIQPPTGLQEWLRTFQSWSGPEKLLALDELIDSCEPTQVKHMMQVIEPQFQRDFISLLPKELALYVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKCREEGIDEPLPPRKRKVAKPGFTHSPWKSAYIRQHRIDTNWRRGDLKSPKVLKGHDDHVITCLQFCGNRIVSGSDDNTLKVWSAITGKCLRTLVGHTGGVWSSQMRDNIIISGSTDRTLKVWNAETGECIHTLYGHTSTVRCMHLHEKRVVSGSRDATLRVWDIETGQCLHVLMGHVAAVRCVQYDGRRVVSGAYDFMVKVWDPETETCLHTLQGHTNRVYSLQFDGIHVVSGSLDTSIRVWDVETGNCIHTLTGHQSLTSGMELKDNILVSGNADSTVKIWDIKTGQCLQTLQGPHKHQSAVTCLQFNKNFVITSSDDGTVKLWDLKTGEFIRNLVTLESGGSGGVVWRIRASNTKLVCAVGSRNGTEETKLLVLDFDVDMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQH9</accession>
    <name>A0A3B5MQH9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">NDUFS1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for catalysing the entry and efficient transfer of electrons within complex I. Plays a key role in the assembly and stability of complex I and participates in the association of complex I with ubiquinol-cytochrome reductase complex (Complex III) to form supercomplexes.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I 75 kDa subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQH9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025776"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026086.1">
        <property type="protein sequence ID" value="ENSXCOP00000025776.1"/>
        <property type="gene ID" value="ENSXCOG00000019267.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153514"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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            <begin position="129"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="611" mass="66619" checksum="6BFCCF4E70671005" modified="2018-12-05" version="1">MEFLLANHPLDCPICDQGGECDLQDQSMQFGSDRSRFTEGKRAVEDKNIGPLIKTIMTRCIQCTRCVRFASEIAGVEDLGTTGRGNDLQIGTYVEKMFMSELSGNIIDICPVGALTSKPYAFTARPWETRKTESIDVLDAVGSNIVVSTRGGEVMRILPRLHEDINEEWISDKTRFAYDGLKRQRLTQPMVKNKSGDLVPTSWEDVLTRVAGALQSVKGSDIAGVVGDLVDAEALVSLKDLLNHLNSDNLCTEEVFPMAGSGTDLRSKYLLNTSIAGIEEADVLLLVGTNPRYEAPLFNARIRKSWLHNELHVALVGKKVDLSYTYDHLGESAKILKEISSGTHPFSQVLAQAKRPVVVVGSSCLQREDGAAIMAAVSTIARNARVSSGLQGTWKILNVLHRVASQVAALDLGYKPGVDSILKNSPKILFLLGADAGCITRQDLAKDSLIIYQGHHGDVGAPIADIILPGAAYTEKSATYVNTEGRAQQTKVAVTPPGMAREDWRIIRAISELAGVTLPYDSLDEVRDRLVEVSPNLVRYDELEEANYFKQANELSKGVNQAVLTESLVPPQLTVKDFYMTDPISRASQTMAKCVKAVTEGAQAVEEPAIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRD0</accession>
    <name>A0A3B5MRD0_9TELE</name>
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        <recommendedName>
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        </recommendedName>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
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        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSXCOP00000022044.1"/>
        <property type="gene ID" value="ENSXCOG00000016465.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="UBCc_UBE2Q"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UBC"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="UBC_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="165"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="323" mass="37086" checksum="575C038532EAC2DD" modified="2018-12-05" version="1">MSVSGLKAELKFLESIFDPNHERFRIIDWKPDELSCQFNVTREKLLIIHCNITESYPSTSPIWFVDSDDPSLAEVLERLEDVRKGSTLHGPTDEVTSEEEEEEEMGEDIDLDQDLDHYDMKEEEPPEGKKSEDDGIEKENLAILEKIRKNQRQDHLNGAVSGSVQASDRLMKELREIYRSQSYKTGIYSVELVNDSLYEWHVKLRKVDPDSPLHSDLQVLKEKEGVDFILLNFSYKDNFPFDPPFVRVVSPVLSGGRRSGWSSAYSIESVIMQINATLVKGKARVQFGANKNQYNLARAQQSYKSLVQIHEKNGWYTPPKEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MS94</accession>
    <name>A0A3B5MS94_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Endothelial cell adhesion molecule a</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MS94"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024066"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024352.1">
        <property type="protein sequence ID" value="ENSXCOP00000024066.1"/>
        <property type="gene ID" value="ENSXCOG00000017969.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157231"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000095">
        <property type="entry name" value="immunoglobulin superfamily member 11 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042757">
        <property type="entry name" value="ESAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44549">
        <property type="entry name" value="ENDOTHELIAL CELL-SELECTIVE ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44549:SF1">
        <property type="entry name" value="ENDOTHELIAL CELL-SELECTIVE ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="232"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="121"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="273"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="296"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="326"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="349"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="424" mass="45514" checksum="AFD3865A742B1F97" modified="2018-12-05" version="1">MFSLCLLAVYDINIIRPNEENLCFLRLGVWANIEMSQTSMEVVKGKLVQLIASYVGNPNADLATSTIIWNFVTDSTINEGSPQFRGRVGFLKAMPSSDVSLYINNTRESDSGRYVCQIIIPSVTSNPAEVYLEVKVPPAVPKCSVTGKPVLKGNVTLTCTSSAGKPVPLYKWRRTSPTSEVFFAPMLNENTGDLKLSNLSSNMSGMYVCTASNTAGSETCSITLDIINTNKVGMIVGATVGSVLGFIFLLCVCVGFLFIFKRRRDNEDDMANEIKEDAQAPKRVSWAKSGMGSDVISKNGTLSSIASSPRHKDNHHHLQQYPQHPASDTASIITATGSTAGYRPPRHQGASTPTHYSYNNSSTLPHGQPASAQAANGSSQLQGQFQPAPSPPPLPTSTITASNIHRMGGVPIMVPAQNQAGSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSF0</accession>
    <name>A0A3B5MSF0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutaminase</fullName>
            <ecNumber evidence="3">3.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GLS</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glutaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSF0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022419"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022693.1">
        <property type="protein sequence ID" value="ENSXCOP00000022419.1"/>
        <property type="gene ID" value="ENSXCOG00000016738.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010463"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:L-glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.210:FF:000001">
        <property type="entry name" value="Glutaminase kidney isoform, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.710.10:FF:000002">
        <property type="entry name" value="glutaminase kidney isoform, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000019">
        <property type="entry name" value="Glutaminase liver isoform, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00313">
        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015868">
        <property type="entry name" value="Glutaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041541">
        <property type="entry name" value="Glutaminase_EF-hand"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03814">
        <property type="entry name" value="Gln_ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544">
        <property type="entry name" value="GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544:SF49">
        <property type="entry name" value="GLUTAMINASE KIDNEY ISOFORM, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17959">
        <property type="entry name" value="EF-hand_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04960">
        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Glutaminase EF-hand" evidence="11">
        <location>
            <begin position="11"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="515" mass="57385" checksum="63FC22656404A7E9" modified="2018-12-05" version="1">RRKAGILPSLEDLLFYTIAEGQEKIPAHKFTTALKSTGLRTGDPRLKECMEMLKVTLKTTSDGALDRHLFKRCVQSNIVLLTQAFRKKFVVPDFQSFSTHINDIYDNGKKLSGGQVADYIPQLARFSPDLWGVALCTVDGQHTVGDTKVPFCLQSCVKPLKYAIAVHDHGTEYVHRFIGKEPSGLRFNKLFLNEDDKPHNPMVNAGAIVCTSLIKQGASNAEKFDYLQVMNFMNKLAGNEYVGFSNAFQSERESGDRNFAIGYYLKEKKCFPEGTDMTSILDFYFQLQLCSIEVTCESASVMAATLANGGFCPITGERVLSPEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSGVAGGILLVVPNVMGIMCWSPPLDKLGNSVRGIQFCTLQDLVSLCNFHNYDNLRHFAKKLDPRREGGDQRSVINLLFAAYTGDVSALRLSSMDMEQRDYDSRTALHVAAAEHAEVVRFLLEACKVNPVPKDRWGNTPMDEAVHFGHHDVVTILRDYHTQYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSU9</accession>
    <name>A0A3B5MSU9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine/threonine-protein kinase 3</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein stk3/mst2 and stk4/mst1, in complex with its regulatory protein sav1, phosphorylates and activates lats1/2 in complex with its regulatory protein mob1, which in turn phosphorylates and inactivates yap1 oncoprotein and wwtr1/taz. Phosphorylation of yap1 by lats2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Phosphorylates 'Ser-14' of histone H2B (H2BS14ph) during apoptosis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSU9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026848"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027177.1">
        <property type="protein sequence ID" value="ENSXCOP00000026848.1"/>
        <property type="gene ID" value="ENSXCOG00000020049.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154984"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21888">
        <property type="entry name" value="SARAH_MST2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06612">
        <property type="entry name" value="STKc_MST1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000040">
        <property type="entry name" value="Dual specificity mitogen-activated protein kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4270:FF:000001">
        <property type="entry name" value="Serine/threonine-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000075">
        <property type="entry name" value="Serine/threonine-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.170.10:FF:000002">
        <property type="entry name" value="serine/threonine-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.170.10">
        <property type="entry name" value="p53-like tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024205">
        <property type="entry name" value="Mst1_2_SARAH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049568">
        <property type="entry name" value="Mst2_SARAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036674">
        <property type="entry name" value="p53_tetramer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011524">
        <property type="entry name" value="SARAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF2">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11629">
        <property type="entry name" value="Mst1_SARAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50951">
        <property type="entry name" value="SARAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="SARAH" evidence="17">
        <location>
            <begin position="420"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="271"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="344"/>
            <end position="405"/>
        </location>
    </feature>
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        <location>
            <begin position="271"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="283"/>
            <end position="294"/>
        </location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="344"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="356"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="380"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00039973"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00059824"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
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    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="474" mass="54199" checksum="A4569B39DE754470" modified="2018-12-05" version="1">FDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKSEHWSEEFTDFVKKCLVKNPEQRATATQLLQHPFISQAKPVTILRDLITEAMEMKAKRQQEQQRELEEEDDNSEETEVDSHTMVKSGSEGAGTMRATSTMSDGAQTMIEHGSTMLESDLGTMVINSDDEEEEEDDQGSMRRNATPQQPIRPSFMDYFDKQDSNKAAQQQENYNRNQPPEQPGYHIQSKNVFPDNWKVPQDGDFDFLKNLDFEELQLRLTALDPMMEREIEELRQRYTAKRQPILDAMDAKKRRQQNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MT74</accession>
    <name>A0A3B5MT74_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MT74"/>
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    <dbReference type="Ensembl" id="ENSXCOT00000027295.1">
        <property type="protein sequence ID" value="ENSXCOP00000026963.1"/>
        <property type="gene ID" value="ENSXCOG00000020138.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177252"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="72" mass="8466" checksum="7492DB65D6C7A5BD" modified="2018-12-05" version="1">MEPLKNQVLQKDVTRRLQVGQNLIDYLNDPDRSPDVEQDKSRLDKTIDELTGWINSSNFKDLMCLQCHFRTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUE1</accession>
    <name>A0A3B5MUE1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sema domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Atherinomorphae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="216" mass="24144" checksum="5E326FE126FDC9E1" modified="2018-12-05" version="1">FSFVKSDAGEIVTEDGRSRCPFNPEYKSTAIMAGELYAGTVSNFQGNEPIIYKSLSQGTALKTENSLNWLQPAFVGSAYIQESLPKGNLVGDDDKIYFFFSEAGKEFDFFDNTVVSRIARVCKGGERVLQKKWTTFLKAQLLCSLPDDGFPFNIIQDMVVFTPSPGDWKNTMFYGVFTSQYKGASGSSAVCSFTMDQVEKAFNGRYREVNRETQQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUK7</accession>
    <name>A0A3B5MUK7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TBC1 domain family, member 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Atherinomorphae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSXCOT00000025236.1">
        <property type="protein sequence ID" value="ENSXCOP00000024932.1"/>
        <property type="gene ID" value="ENSXCOG00000018611.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00934">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01269">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TBC1 domain family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TBC1 domain family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000076">
        <property type="entry name" value="TBC1 domain family member 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF3350"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF14">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11830">
        <property type="entry name" value="DUF3350"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1028"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="5">
        <location>
            <begin position="338"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="6">
        <location>
            <begin position="862"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="561"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="676"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="725"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="573"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="593"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="611"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="634"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="727"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1054" mass="118810" checksum="278EED48E99B7ED0" modified="2018-12-05" version="1">MEIQEEKESRTDKRFALTYVGWCSLDRRTTLPMLPWLVAEIRRRSERGDCGPVMQPREVQLLLNTPFVRCVPSNSSNSSVFIFEHKAQLISRFIHNSNDLTCFSYLLRGQPDNPESEMSCHVFKACDPNQVPEVISSIRQVSKSALKEDAKPKQEADEAFYNSQKFEVLYCGKVTVGHKKASSTLIDDCVEKFRQHETERKRLRLLNGQRGSTETIPVDLLIAGDGDPLFTALSRTTEEPEAPGVEDLITGICLPSSASQSSLRGSFPECILEDSGFEEPQEFRTRCSSLAGSLQRKPGEGVIMGPTRRRHASAPNNVQPSDSDKNRTMLFQVGRFEVNLISPDSKTVVLEKNFKDISSCCQGVKQTDHFGFICRDQSESGPSQYMCYVFQCANESLVDEVMLTLKQAFSTAAALQSNKTPVQLCEACPMHDLHKLCERIEGLYPPRAKLAIQKYLSLLTDNEQAEIFERVQRLKPGSDQEENELAIMYLRQLCESKQKSHLHIGETPQVSGGDGSATGSRFKLDILKNKARTSLTSSLENIFARGASRMRGRLGSMGSISSFERQDEESPGHSPPGSPPAFPDEELESGPQFRRRAHTFSHPPVRKRISFESQPANQTKQAPLRRQHSVNPELLQTSHVSRTRSISEGESSFSLPSTFSAPTFLKSFYQGSLGSLTDSGSFKSNGEGRKRTLSSCSNDSLSGGLPLTPRRVSWRQKIFLRVASPMNKPSASMQDTDGSDATELLPLSPRASDPGLDPLRRLLSPASDQPADKKPKKTGADYRALWKTAIHQQILLLRMEKENQRLEASRDELHIRKTKLDYQEVCTCSKEAQALWERKLTAPGRTTNPQDKEDIYRAVCQGVPKSRRGEVWLLLSHQHRLQHRLPQRQQAPDTPYYDLLKQLTAQQHAILVDLGRTFPTHQYFSAQLGAGQLSLYNLLKAYSLMDTEVGYCQGISFVAGVLLLHMREEQAFDLLKFLMYDLGIRRQYRPDMVSLQIQMYQLSRLLHDYHRELYNHLEEYEIGPSLYAAPWFLTLFASQFPLGFVSCIFGKART</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXH2</accession>
    <name>A0A3B5MXH2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXH2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024700"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024999.1">
        <property type="protein sequence ID" value="ENSXCOP00000024700.1"/>
        <property type="gene ID" value="ENSXCOG00000018452.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160441"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02257">
        <property type="entry name" value="Peptidase_C19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF28">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="4">
        <location>
            <begin position="41"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="315" mass="35506" checksum="14E75AC32F389BF6" modified="2018-12-05" version="1">CFFQEKCQISWEPQDYFHGTSYNLRSRPCTPSNKQGKPGVVGLDNIGNSCYFNAVMQCLCSTMPFVEDLLSQETSRELAKYCGIVQVFIELLEQMWQGSSTSCSPVQVMSALPSIFPQFDSYTQQDAQELLLHLLDALHEDLKKVHQNLSSANTNQSRSSKTESTIVSDLFEGQLSYLTSFLHCGHEARNSQTFTILSLPIPNGNKKCSIQDCLSLFFEETVLTGAEQTYCSDCGLRREATVQTYLNKPPEILILHLKRFGCKGRRQVKLSANVSFSMELDITPFLSSSVRSWSSYHLYAVVVSDQTTECNKLHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZ42</accession>
    <name>A0A3B5MZ42_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Ceramide synthase 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>sphinganine + octadecanoyl-CoA = N-(octadecanoyl)-sphinganine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:57817"/>
            <dbReference type="ChEBI" id="CHEBI:67033"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:36548"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MZ42"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026055.1">
        <property type="protein sequence ID" value="ENSXCOP00000025745.1"/>
        <property type="gene ID" value="ENSXCOG00000019241.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234515"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016439">
        <property type="entry name" value="Lag1/Lac1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560:SF43">
        <property type="entry name" value="CERAMIDE SYNTHASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560">
        <property type="entry name" value="LONGEVITY ASSURANCE FACTOR 1 LAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005225">
        <property type="entry name" value="LAG1_LAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="11">
        <location>
            <begin position="89"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="304"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="330"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="352" mass="41512" checksum="120C8B86C7E7FA52" modified="2018-12-05" version="1">MAGILAWFWNERFWLPHNVTWADLKNTDEATFPQAEDLYLACPLAFCIFMIRLVFERWVLNFSTKRLKISLCKFLIKKEAKRSEIFHLQLTWICRWRFTFYLYIFTYGVRFLKKTPWLWNTKECWYNYPYQPLTVDIHYYYILELSFYLSLLFSRFTDIRRKDFLIMFLHHVAAISLIVFSYVNNMARVGTLVMCLHDAADVLIEAAKMANYAKCQILCNLLFAMFAILFITSRLGVYPIWILNTTLFESWEIVGPYPSWWVFNLLLVLLQLLHSFWSYLIVKTACRAISKGKVGKWNPLHVSKDDRSDIESSSDEDDSPPPSQKHHSSTTNGTNKNGTNGYLTGVPYPDEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KLW6</accession>
    <name>A0A3B5KLW6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">[heparan sulfate]-glucosamine N-sulfotransferase</fullName>
            <ecNumber evidence="5">2.8.2.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Glycan metabolism; heparan sulfate biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycan metabolism; heparin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sulfotransferase 1 family. NDST subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.8" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KLW6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001108"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001121.1">
        <property type="protein sequence ID" value="ENSXCOP00000001108.1"/>
        <property type="gene ID" value="ENSXCOG00000000901.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157168"/>
    <dbReference type="UniPathway" id="UPA00756"/>
    <dbReference type="UniPathway" id="UPA00862"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019213">
        <property type="term" value="F:deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015016">
        <property type="term" value="F:heparan sulfate N-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030210">
        <property type="term" value="P:heparin proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021930">
        <property type="entry name" value="Heparan_SO4_deacetylase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056793">
        <property type="entry name" value="HSNSD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF45">
        <property type="entry name" value="BIFUNCTIONAL HEPARAN SULFATE N-DEACETYLASE_N-SULFOTRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12062">
        <property type="entry name" value="HSNSD-CE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25119">
        <property type="entry name" value="HSNSD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Heparan sulfate-N-deacetylase N-terminal" evidence="20">
        <location>
            <begin position="78"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Heparan sulphate-N-deacetylase deacetylase" evidence="19">
        <location>
            <begin position="308"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfotransferase" evidence="18">
        <location>
            <begin position="602"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="active site" description="For sulfotransferase activity" evidence="16">
        <location>
            <position position="611"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="709"/>
        </location>
        <ligand>
            <name>3'-phosphoadenylyl sulfate</name>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR637359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF12062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF25119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="806" mass="92589" checksum="75F0A65EC4D9C7EF" modified="2018-12-05" version="1">MTVAVRFRRHLRRLLLLLASCCLISLLLSAYFLFSNSSPSMHLGQSAEPACSQPLSMSPYRQLPYPYPPNPPHTHVHTDPVVLVLVESQYSQLGQDIMAILESAHFQFRMEIASGKGDLPPLTEKGRGRYSLIIYENLLKYAHADTWNRQLLHQYCTEYRVGIIGFYRSTENSPSVLKLRGLPLVLRTNQALWDCCVVSSSSLLHLTKPGTDRGALPGEDWTSFSSNHSTYQAVLLARAKEGAGAGSGDNAGPGFSLGLQATVVQDMGLYDGVRRVLFGQGLGYWLHRLILVDAISYLTDRKLTLGLDRHVLVDIDDIFVGKEGTRMNAKDVKALLETQKQLRSYISNFTFNLGFSGKFYHTGTVEEDEGDDLLLKYVDEFWWFPHMWSHMQPHLFHNESSLLEQMVLNKEFALEHNIPVDMGYAVAPHHSGVYPVHLQLYEAWRRVWNIRVTSTEEYPHLKPARYRRGFIHNNIMVLPRQTCGLFTHTIYYKEYPGGPKELDRSIRGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFVHLVSFLRSWTNLKLHTLPPLQLAHKYFQLFPEQRNPLWQNPCDDKRHKDIWSKEKTCDRLPKFMVVGPQKTGTTALYLFLLMHPSISSNFPSPKTYEEVQFFNTNNYHKGIDWYMEFFPVPSNVSTDFMFEKSANYFPSEETPRRAAALLPKAKIITLLINPSDRAYSWYQHQRAHEDRTALQFTFYEVISARKGASAELRSLQSRCLLPGLYATHLERWLMYYPSNQVMIIDGHQLRNDPAAVMDEVQRFLGVTPHFNYSQTLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KMI1</accession>
    <name>A0A3B5KMI1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KMI1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001313"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001336.1">
        <property type="protein sequence ID" value="ENSXCOP00000001313.1"/>
        <property type="gene ID" value="ENSXCOG00000001065.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234530"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF127">
        <property type="entry name" value="NITR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="19"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="140"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="337" mass="37930" checksum="B78372FA73BFB16E" modified="2018-12-05" version="1">VVRICSLFDSIVIEMLKVPDKIPLTEAELGQTVNLTCDTSGHDDGLFFWYKMNYGHMVQTVVRGTFSDLKREKDFSNSRFQVENVGDINSLIIRNVNKEDEATYLCQTGAAYNLAFVSGTNLIKSFRVKQTSDMKSSLLGTSVTLQCSLHSEKKEKESTDQCAAEHDVFWFRAESESNPGFIYADKTHCGKQAGSSCEYRLSKTIRNSSDAGTYYCAVVTCGEILFGEGTKVETKLFLYFIVLLGGLLACSVLVNITLIFQRRKPRQLRESHKGKFVVCIILTLTDDAEDGLNYVALNFSSRKPIRWKSKRETSNDSTYSNIKLSMKGSIHQRDNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KNP7</accession>
    <name>A0A3B5KNP7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Gypsy retrotransposon integrase-like protein 1</fullName>
            <ecNumber evidence="3">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000097"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000097.1">
        <property type="protein sequence ID" value="ENSXCOP00000000097.1"/>
        <property type="gene ID" value="ENSXCOG00000000074.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01060000248608"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="entry name" value="RT_LTR"/>
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        <property type="entry name" value="Retrovirus-related Pol polyprotein from transposon 297-like Protein"/>
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        <property type="entry name" value="Transposon Tf2-6 polyprotein"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
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        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Chromo-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
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        <property type="entry name" value="DUF4939"/>
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        <property type="entry name" value="Integrase_cat-core"/>
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        <property type="entry name" value="Integrase_H2C2"/>
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        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
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    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
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        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
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        <property type="entry name" value="RNaseH-like_sf"/>
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        <property type="entry name" value="RT_dom"/>
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    </dbReference>
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        <property type="entry name" value="SH3_Tf2-1"/>
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        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RT_POL"/>
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    <keyword evidence="17" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="16" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="10" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="481"/>
            <end position="660"/>
        </location>
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        <location>
            <begin position="999"/>
            <end position="1158"/>
        </location>
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        <location>
            <begin position="1300"/>
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        </location>
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        <location>
            <begin position="1"/>
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        </location>
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            <begin position="1"/>
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        </location>
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        </source>
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            <dbReference type="ARBA" id="ARBA00039658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1362" mass="152891" checksum="481DB905DA0D382C" modified="2018-12-05" version="1">GSESWDATEKGQATTSADAIRGALSDQQALIQLHDSALRELGNRQAETNRRLTELSNFLQNSLPQDTNQPSVSPSAPDPLVRSGFSEVRPATPDKFSGDIKKVTGFILQCTIVFNHSPQSFPNDDRKISYVLSLLTGRALEWAEARFTTAVNYGCTYPEFLTELKQVFCQETEGTSSSRDLHGLKQGQKSVSDFAIDFRIKAAASGWNMVALKSAFFHGLNEQIKDELATLDEPESLNDFINLSIRLDNRIRTRARERTRNTRLTPEERQRRLRSQLCIYCPAVKAGKLPAPSTPKPRLTFPVTLFANNDSLSVSVLIDSGCEQNLISSDIIHQLSVPTEPLPVPLRVAALDGKGLPQITHRTKPLRLLISGNHTEEIIFYVFPTISSPIVLGFNWLFKHNPQIDWVEGRITSWSSKCHTTCLRSAVPSQPTRSTMDEVDPSDVTAVPSEYHELITVFSRDKALSLPPHRPYDCDYINKSLAAGIIRTSSSPLGAGFFFVGKKDGSLRPCIDYRGLNQITIKNKYPLPLLSSAFEPVQGATVFSKLDLRNAYHLLRIREGDEWKTAFKTPMGHYEYLVMPFGLSNAPSFFQALVNDVLRDFINVFVFVYLDDILIYSRTLVEHQEHVRLVLQRLLENKLYVKAEKCEFHRPSVTFLGYVLESGQIRAAEDKIAAVLDWPQPETRKQLQRFLGFANFYRRFIRNYSQIASPLTALTSTKRVFSWTPQAEAAFTELKSRFSKAPVLVQPDPSRQFVVEVDASDIGVGAVLSQITAQDQKLHPCAFFSRRLSPAERNYNVGDRELLAIKLALEEWRHWLEGAEHPVLVWTDHKNLSYLQSAKRSNPRQSRWSLFFARFNLSISYRPGSKNVKPDALSRIHSPDDSEESLAPILPPSCTVGGVTWEMEDILRQAHQTEPPPEACPPGKQYVPSAVRARFLQWIHASKFAAHPGVSRTIGLVNRRFWWGSIHKDVKEYVSACAVCARNKSSNRPPAGLLQPLPIPGRPWSHIAVDFVTGLPRSKGMTTILTIIDRFSKACHLVPLRKLPTAAQTAKLLIKHVFKLHGIPQEILSDRGPQFISQVWRQFCSALGARVSLSSGYHPQTNGQTERMNQELEAALRCLTSTNPDDWAPHLPWVEYAHNCHTSAATGLSPFEVVLGYQPPLLPADESNISVTSVQHHIRRSHKIWKDTVSALNNSSASNKRLADRRRIPAPTYSVGQKVWLSTRDIPLKSMSRKLSPRFIGPFEILALVGPAAVRLRLPSSMRIHPTFHVSHIKPVLSSELCPPAEPPPPSLDGRGDAIFAVRRLVASRRRGRGYQYLVDWEGYGPEERSWVPGSFIVDNTLIREYWDSQPSSSARPPGGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KPT3</accession>
    <name>A0A3B5KPT3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyrin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KPT3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000480.1">
        <property type="protein sequence ID" value="ENSXCOP00000000472.1"/>
        <property type="gene ID" value="ENSXCOG00000000419.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000176860"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004020">
        <property type="entry name" value="DAPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02758">
        <property type="entry name" value="PYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01289">
        <property type="entry name" value="PYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50824">
        <property type="entry name" value="DAPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="97" mass="11613" checksum="ACF0E7023737F032" modified="2018-12-05" version="1">MAKEDILNTLEELGQEEFEKFKWFLQQPETLPELPAIKKKCLEGAKTLDIVDLMMQIYTLDRCREMICKILEKINRNDLSFYQENIKDLQQNIYLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQM4</accession>
    <name>A0A3B5KQM4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQM4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000185.1">
        <property type="protein sequence ID" value="ENSXCOP00000000180.1"/>
        <property type="gene ID" value="ENSXCOG00000000170.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161376"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="150"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="185" mass="21146" checksum="EE024861EB817972" modified="2018-12-05" version="1">MSGNHFKGHEVSCCIKYFIFGFNILFWVRTIHCHSYPLFQEGARLIFTLWLFLNFSFHSYRCLVITVALFGRWGPDRSSCSCTHAANRLFVIMRADREKCGVPFSCCTKDPAEDVINTQCGYDIRAKPQKDYINVKGCVPQFERWLQDNLTLVAGIFIGVALLQIFGICLAQNLVSDIEAVRASW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KR38</accession>
    <name>A0A3B5KR38_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KR38"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000352.1">
        <property type="protein sequence ID" value="ENSXCOP00000000345.1"/>
        <property type="gene ID" value="ENSXCOG00000000309.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169709"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050504">
        <property type="entry name" value="IgSF_BTN/MOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100">
        <property type="entry name" value="BUTYROPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100:SF151">
        <property type="entry name" value="ICOS LIGAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="160"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="186" mass="20912" checksum="EF8B747150D41B56" modified="2018-12-05" version="1">LTFVTSGVDINGCISSYFEGRINLHCYISCTKIIFYCWIRSDLGSEYVLLYRNDQLDLENQHVMFKDRVDLQDRQMKDGNVSLVLKNVTTDDGGAYECRIIQTETNSRRKTIIIINLIVTSPGNRDGPAGLIGLVVPLVILLPLVIIVVYRNHEKLSGLCSKSSQSSEEPPPSQQPLTDQNRPPEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRD0</accession>
    <name>A0A3B5KRD0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SCM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRD0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000435"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000443.1">
        <property type="protein sequence ID" value="ENSXCOP00000000435.1"/>
        <property type="gene ID" value="ENSXCOG00000000380.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159407"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09578">
        <property type="entry name" value="SAM_Scm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000018">
        <property type="entry name" value="Polycomb protein scmh1 isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050548">
        <property type="entry name" value="PcG_chromatin_remod_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047531">
        <property type="entry name" value="SAM_Scm-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12247:SF84">
        <property type="entry name" value="SEX COMB ON MIDLEG-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SAM" evidence="8">
        <location>
            <begin position="144"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="97"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="212" mass="24007" checksum="12ED9B1A4216FCC6" modified="2018-12-05" version="1">MQNTRLTDNLQRNQKTNLLTISRLTLLDIVRTLLINFSVVSVWKSYFIPSLSHWIPVFLSYSSWCTSRSGFFHISNTSLLFQLLLILDLHADLIPTAKSPATPPRPQGCPRSPSKIPDHSASSTTGPDHMKQDRETSGNNASSWSVDEVIRFVQEADPQTLGPHVDLFRKHEIDGKALMLLRSDVIMKYMGLKLGPALKLCHHIEKLRQTKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTF8</accession>
    <name>A0A3B5KTF8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Elongin-B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Elongin 18 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">RNA polymerase II transcription factor SIII subunit B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">SIII p18</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Transcription elongation factor B polypeptide 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Heterotrimer of an A (ELOA, ELOA2 or ELOA3P), ELOB and ELOC subunit. The elongin BC complex interacts with EPOP; leading to recruit the elongin BC complex to Polycomb group (PcG) target genes, thereby restricting excessive activity of the PRC2/EED-EZH2 complex. Component of multiple cullin-RING E3 ubiquitin-protein ligase complexes composed of Elongin BC (ELOB and ELOC), a cullin (either CUL2 or CUL5), a catalytic subunit (either RBX1 or RNF7/RBX2), as well as a substrate adapter protein that can be either ASB2, ASB9, ASB11, KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B, FEM1C, LRR1, PCMTD1, SOCS1, SOCS2, SOCS5, SPSB1, SPSB3, ELOA, VHL, WSB1 or RAB40C. As part of the Elongin BC E3 ubiquitin ligase complex; interacts with NRBP1. May also interact with DCUN1D1, DCUN1D2, DCUN1D3 and DCUN1D5. May form oligomers as a KLHDC2/KLHDC3-ELOB-ELOC complex; this interaction is autoinhibitory for the E3 ligase complex as the substrate-binding site of KLHDC2/KLHDC3 is blocked in the oligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the Elongin B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTF8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001255"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001273.1">
        <property type="protein sequence ID" value="ENSXCOP00000001255.1"/>
        <property type="gene ID" value="ENSXCOG00000001028.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018316"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070449">
        <property type="term" value="C:elongin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030891">
        <property type="term" value="C:VCB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01788">
        <property type="entry name" value="Ubl_ElonginB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000108">
        <property type="entry name" value="Elongin-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039049">
        <property type="entry name" value="ELOB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13248:SF4">
        <property type="entry name" value="ELONGIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13248">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="18">
        <location>
            <begin position="8"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00083653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="126" mass="14332" checksum="7C97CDB1BF586DBB" modified="2018-12-05" version="1">MIVLIIFKDVFLMIRRHKTTIFTDAKECTTVYELKRIVEGILKRTPEDQRLYKDDQLLEDSKTLGDCGFTNQTARPQAPATVGLAFRVNDEMFEQLHIEAFSSPPELPDVMKPQDSGSTANEQAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KUL4</accession>
    <name>A0A3B5KUL4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Si:ch211-215a10.4</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KUL4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003813"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003856.1">
        <property type="protein sequence ID" value="ENSXCOP00000003813.1"/>
        <property type="gene ID" value="ENSXCOG00000002995.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008658"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018790">
        <property type="entry name" value="DUF2358"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31094">
        <property type="entry name" value="RIKEN CDNA 2310061I04 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31094:SF2">
        <property type="entry name" value="RIKEN CDNA 2310061I04 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10184">
        <property type="entry name" value="DUF2358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="175" mass="20431" checksum="8E7CC6962A0A75B0" modified="2018-12-05" version="1">MYERLRLELPNFFMTNHDYTMYSNDVEFINGLLNMKTRGLMGYRLTLTLWRFLSLCYYTEARLDVLKLTKHMEDGTIKARWRIKGLPFHSGLLRFYRKDKSHLYRSYDAFSTFYIGHDGLIHCHKVEKVMPAQPPVLPRVTSLLAGALVALGVQEHRPALNLLPPILSSLRQSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KV89</accession>
    <name>A0A3B5KV89_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MID1 interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SPOT14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KV89"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001526"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001551.1">
        <property type="protein sequence ID" value="ENSXCOP00000001526.1"/>
        <property type="gene ID" value="ENSXCOG00000001242.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00500000044890"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Lipogen_MT_Stabilize_sf"/>
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        <property type="entry name" value="Spot_14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14315:SF19">
        <property type="entry name" value="MID1-INTERACTING PROTEIN 1-B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14315">
        <property type="entry name" value="SPOT14 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Spot_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="98" mass="11320" checksum="37517E8F724CB4D5" modified="2018-12-05" version="1">MMQLSETSKQKNSLFNAMNRFIGAVNDMDQTILVPSLLRDVPLEEDREMGVLKSDVEDGDMFSYYELLKSIRSDMEWGVRCAHTRKLVMWGRSGLNQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KVZ0</accession>
    <name>A0A3B5KVZ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Reelin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KVZ0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001781"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001808.1">
        <property type="protein sequence ID" value="ENSXCOP00000001781.1"/>
        <property type="gene ID" value="ENSXCOG00000001441.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165599"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08544">
        <property type="entry name" value="Reeler"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4060">
        <property type="entry name" value="Reeler domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051237">
        <property type="entry name" value="Ferric-chelate_Red/DefProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002861">
        <property type="entry name" value="Reeler_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042307">
        <property type="entry name" value="Reeler_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45828">
        <property type="entry name" value="CYTOCHROME B561/FERRIC REDUCTASE TRANSMEMBRANE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45828:SF32">
        <property type="entry name" value="SI:DKEY-251I10.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02014">
        <property type="entry name" value="Reeler"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51019">
        <property type="entry name" value="REELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017255178" description="Reelin domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Reelin" evidence="2">
        <location>
            <begin position="12"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="188" mass="20993" checksum="34309707F69BADC1" modified="2018-12-05" version="1">MLLLCLLTLQFLCIVDCYPNGAPTGACKDMMPQHAGVVPQTSPAPYTILTNMKRYLPGMPVTVTIIGPQYRGVLLEARREHFNDAYGSWKIPPPDTKFLQCSGNPQGAITHANTNLKSNTTVYTWIPHDIMHPVYFMATVAQQRTVYWINVRSNILPSGKYSTPVSRSRSLYPKPLTALPIMTKPMVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KW50</accession>
    <name>A0A3B5KW50_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ras-related protein Rab-19</fullName>
            <ecNumber evidence="4">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KW50"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002762"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002799.1">
        <property type="protein sequence ID" value="ENSXCOP00000002762.1"/>
        <property type="gene ID" value="ENSXCOG00000002197.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156717"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01864">
        <property type="entry name" value="Rab19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000887">
        <property type="entry name" value="Ras-related protein Rab-19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048040">
        <property type="entry name" value="Rab19/43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0636">Prenylation</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00057434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="210" mass="23640" checksum="9AFF3727075D1753" modified="2018-12-05" version="1">MQSAESEVEDSFDFLFKIILVGDSDVGKTCMVQRFKSGIFIEKQQNTIGVDFTVRTLDIDGKKVKMQVWDTAGQERFRTITQSYYRSAHGAMVAYDISRRRTFESVPHWIREVEQYGAANVVVILVGNKSDLHAQRQVLFEDACTLAEKNSLLAALETSAKEAQNIEAAFILMARELLARNGMAITDQNSHCSPQFMKGPSLKTMCRCLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KWA2</accession>
    <name>A0A3B5KWA2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RWD domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Protects DRG2 from proteolytic degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RWDD1/GIR2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027887189.1">
        <property type="nucleotide sequence ID" value="XM_028031388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KWA2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002812"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002849.1">
        <property type="protein sequence ID" value="ENSXCOP00000002812.1"/>
        <property type="gene ID" value="ENSXCOG00000002220.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114153087"/>
    <dbReference type="CTD" id="51389"/>
    <dbReference type="GeneTree" id="ENSGT00390000009168"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23816">
        <property type="entry name" value="RWD_RWDD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000064">
        <property type="entry name" value="RWD domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040213">
        <property type="entry name" value="GIR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12292">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0007">Acetylation</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="7">
        <location>
            <begin position="10"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00056432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00072006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="240" mass="27798" checksum="DBA03A3F17F43052" modified="2018-12-05" version="1">MTDYVEEQRNELEAIESIYPDSFTVLSEEPTSFTITVTSDAGENGETVEATLKFTYVAEYPDEPPLWEIYSQENLEDGDLENILTLLKQQAEENLGMVMIFTLVTAVQEKLNEIVDAMKNRREEEKLRKEKEAEEAEKVKFQGTVVTIENFLAWKAKFELEMAEFRRKKQKEEEQAGKPRLTGKQLFERDHNLDTSDIQFLEDAGNNVEVDESLFQDIEDLDLDEDDPNFDPLDMDSDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KWF0</accession>
    <name>A0A3B5KWF0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KWF0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002384.1">
        <property type="protein sequence ID" value="ENSXCOP00000002350.1"/>
        <property type="gene ID" value="ENSXCOG00000001881.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160428"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="189" mass="20384" checksum="E14B9C40282035C2" modified="2018-12-05" version="1">MEASTNGSSFGIDSLLSHRPGSPLSKADGLLPGECRSPLEFSPRSDVESGCSSPPSPRRECVEEAAQRQVHSAGLPPHLQHAQISAGAQQRTVTSSFLIRDILADCKPLAACAPYSSNGQPTQEAGRLAAKIADDFIEKIHSNSSSDSEYKGSNFSRNIPFSLKTFYSGNRKKQTTKITFCMLSINMLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KY31</accession>
    <name>A0A3B5KY31_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Prothymosin alpha</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pro/parathymosin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KY31"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000459"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000467.1">
        <property type="protein sequence ID" value="ENSXCOP00000000459.1"/>
        <property type="gene ID" value="ENSXCOG00000000402.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183307"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004931">
        <property type="entry name" value="Pro/parathymosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22745">
        <property type="entry name" value="PROTHYMOSIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22745:SF13">
        <property type="entry name" value="PROTHYMOSIN ALPHA-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03247">
        <property type="entry name" value="Prothymosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="41"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="11803" checksum="03CD96935D92FBB6" modified="2018-12-05" version="1">MADTQVDSGSDISAKDLKEKKLAEEKENGKDAATNGKENEENGEPEVDEEEVDEEEEEEEEDDGEDEEDEEDDEDEIEGGTKRAADDDDEDDEDDVETKKQKTDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYX0</accession>
    <name>A0A3B5KYX0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Golgin B1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000002831"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002868.1">
        <property type="protein sequence ID" value="ENSXCOP00000002831.1"/>
        <property type="gene ID" value="ENSXCOG00000002244.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175917"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026202">
        <property type="entry name" value="GOLGB1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18887">
        <property type="entry name" value="GOLGI-ASSOCIATED PROTEIN GCP360-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18887:SF4">
        <property type="entry name" value="GOLGIN SUBFAMILY B MEMBER 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1865"/>
            <end position="1882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1583"/>
            <end position="1606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1626"/>
            <end position="1652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1733"/>
            <end position="1757"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="40"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="211"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="398"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="525"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="869"/>
            <end position="1494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1759"/>
            <end position="1793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1583"/>
            <end position="1597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1631"/>
            <end position="1641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1884" mass="215572" checksum="CEBBC7CFE22F5CC1" modified="2018-12-05" version="1">MLKWFSGEEGEPGSSVSPEFEMAERLIQTEQLVSQLKELIREKDATLRSKDDQLKELKERLQRLQVERESLESKMEAEKHVMRAQLRDLMEKHQVELQRLSAQHQDQMERLQTELLGQLEERRTASPAVAPPVCQEASGTGNLPTDPASVQRMAELEAEAKQKTVEASRSEAKFLKMKAWSKSRIRQLEEELKKSQAGAAPPDLTALRGRITALEEEREENQWKVDQYEELKAKSEMLEAKLVVYEEQQRTLQAELEQFTKRAASQASESGSTDDTQSKVLEWQEMVAEAASARGRVREEREERAAMALRISHMEEEREGETAADDWFFPGCSDPALATRQQELEEELAQARGLGRPGAKKLLAPAHRSLQVGANSSTTPMEGENMGGWWPEFSTPDTDGLRSVVEELELERNQLQEQILSLEERCQDLEDRLQLQARIEALQVTFDVDEEEQPCWVSQNEMEKLQSQLASVRGQHSRDTEKHQLLVNSLSEQLKGLTDTQESLQSSLIEKENMLAKTSEKLELIDSLRETLSQKEIQVKELSDKLLQTEHSLENVTKRRSSSEKQCSELKTEAADLTQKLSALKEKAQKQEVTIETLQAELDQTSEELDKLNTTHLEERAQLIHDLQSCEREIDSLKDVLLEKDREISSLAGNMAEYAEQLSLLKQEVKEKEESLVQVENALRKAEREATIIRDSQNSDQQTLNGKITELMGKLKDAEAELEKTKEEGESKVAEVEHLLQQAEGDKKTIQELRGDIQKLNLKDKEQVQERELKSSKDEQNTLLAQVEKYGNQVERLSSRLEEKLEAIRSLEEQIRTADKLVEEERQKFDSEKARLNNEIQNKSEGFSKVEEILKSTETQKQDSNSELLLEKTNECSRLLEALREKEEEASRMRLQVEKTERTAADLQSKTETQQSLLTQLEETLFLLREQESSLKSGLMEKDRMLKQKEEEESRLEAEAGSLNEELSELKHRLKEQEETCQTHKDQLNDRNQAVKSLNDQVNVFRENTRKLESEAEWRKTKLDDLNSHLQTLTEENQNLRFACESKENQISQQRQTVSELTQQLEASAEQNSQIRTQIINLTADNQRLQEELGKNSQLVSDLAAEKSFLQEKASSLENQIQDNQKTISALTDEKEELRKILTESQESNSAGLLEKTNECARLSRSLRETEEHLQSLQEQVSQLKDTEKTVSEQRTQLEARQNQLLQLQDTVSMLQEQGSVLKAALMEKDSTLNQMAEECRVYQNELLTQRELASKLQEEAESVRRETCEMRQQLEQKEQAFQEVTHQLQSQREELKKRSESVFSLSSQIGAMNQSAAEMEAEISSLKASLQKLSAENEQQLQQSEAEVTDLRDGMQALTDQNCRLKAELQKMASELELISRLRAEISDREKELRNVQSEAETMRSTMQEKDENLEKLQAFIQQQDQQLKQIKDHNDTLQKQLSELQETTSNLRGQVETLTSESGVLKDALEKKDSLQVQASEAERLRAALGEKDAALLERSRSVEEAQRRADEASLFKSQFMESTEMASQLQSQNQLLSAKSENLKRSAEEMQRAFTNLQEKYAQSLEELQDVRKRLAERTEEVDDLRAQMDDSSRGRQTAETTVETLRTEVSLIRDKLDRSQALNSSLSKEKEDAHQKETAALAGQSSQEAAVSRLEAEKMQLYRDLQRCVYEIQQRDQYFHQLNTKLQQAVEEKAAVAGQLRAVSQTLRDSQNRCHWLESQIQGQPGVCTQQGSAEVAPGAPQERSSDSVSEAAEASQLRERLVEVELSLADERARRESAEEALRLAEDRAKSAASGLSRDNQRDFSIEMEAEEDWEALSLDPNQPLITRKVKGGMVACRQWLRGRSLYFSRLLTGRARSRYFFLAYLLTIHVVVLMCSSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZG0</accession>
    <name>A0A3B5KZG0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZG0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003011"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003048.1">
        <property type="protein sequence ID" value="ENSXCOP00000003011.1"/>
        <property type="gene ID" value="ENSXCOG00000002388.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175984"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="212" mass="23677" checksum="0026978F163CB16A" modified="2018-12-05" version="1">MEAHTISFAKNDKEPKLAFESVVASELYPCVLFYSSNPGEKVALRDLQMRGMPSNLLPGEPLCSPRTTVLLESTVHLLRRLHQCDHWSSHINQYIHSHLELIVHKGRFNFSCKLKKKRLSNPCPEMEDKDISGEETGKPAAHRHILSEAKLTALCIQVWPALAVIGGVDSGLRAGGLCLHKPSGRRAIMLGVKCCLTAITWRPSRLTHCPWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZG2</accession>
    <name>A0A3B5KZG2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZG2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003016"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003053.1">
        <property type="protein sequence ID" value="ENSXCOP00000003016.1"/>
        <property type="gene ID" value="ENSXCOG00000002392.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166813"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000006">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042469">
        <property type="entry name" value="HECTD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46654">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46654:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="11">
        <location>
            <begin position="104"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10">
        <location>
            <position position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="457" mass="51611" checksum="1D92482058EE92B1" modified="2018-12-05" version="1">QTTSQSNTNMNSCKRGGGLLRIGKCKRFFSGTKTLGCPNSMLCFQLTNHVQRPKGILGPLFGMLIVLLELFHRVCFKPSLIPSHRGRSSKPIFVQIAKQVVNLNPLELRLPSRAWKVKLVGEGADDAGGVFDDTITEMCQELQSGVVDLLIHTPNSFADVGCNTDRFLLNPGAHSEDHMVQFRFLGILMAVAIRTKKPLDLHLAPWVWKQLCCMPLSGADLEEVDLLTYRTLQDSFMAHSADGRLVPVVPRGQNISLTFSNRTEYVERALDYRLHEMDSQVDAVREGMSTIIPVPLLSLLTAQQLEQLVCGLPEVSVEMLKKLVRYRDITENHQLIRWFWQSLKEFTNEERVLFLRFVSGRSRLPSNLADISQKFQIIKVDRPINGLPTAQTCFFLLRLPPYTSQAILAERLRYSIHNCTSIDMDNYMLTHNTEPAESSDTEDTVEKFAVDAAADYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L0V9</accession>
    <name>A0A3B5L0V9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Complement C1q tumor necrosis factor-related protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">C1q/TNF-related protein 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="6">Homomultimer. Forms trimers, hexamers and high molecular weight oligomers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L0V9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003516"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003555.1">
        <property type="protein sequence ID" value="ENSXCOP00000003516.1"/>
        <property type="gene ID" value="ENSXCOG00000002774.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161832"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="complement C1q tumor necrosis factor-related protein 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050822">
        <property type="entry name" value="Cerebellin_Synaptic_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923">
        <property type="entry name" value="CEREBELLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923:SF118">
        <property type="entry name" value="COMPLEMENT C1Q TUMOR NECROSIS FACTOR-RELATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0202">Cytokine</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017288501" description="Complement C1q tumor necrosis factor-related protein 4" evidence="10">
        <location>
            <begin position="27"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="11">
        <location>
            <begin position="33"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="11">
        <location>
            <begin position="216"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="176"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="201"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00084029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="377" mass="42292" checksum="56E7CF3FBB1C6132" modified="2018-12-05" version="1">SAELCWSVTNVLIGLLCLGQPGGAWGLARLLDHAELRSAFSVSRTNSMEGGPKMTVTFDMVYVNIGGDFDAKAGLFRCRIPGAYYFSFTVGKFPHKDLSVMLMKNRNEVQAIVYEENAGEERKVQSQSVMLQLEFGDTVWLRLHGDPRYALYSNTGHYTTFNGYLIYPDTYSYQTHHHQHQPIYNPQQDTEDAKSRPGDTEKEEEEENSQEYDDEEDDGRSAFSVGRTQSILGIDQVGVPQHRPISFDTAFVNIGEDFNVTEGVFTCRVPGAYYFSFNVGKLPMKTLSVKLMKNRLEVQAMIYDDSQGEKALQSQSLMLALKSGDVVWLYSHQRDGFGAYSNHAKYITFTGFLVYPDISPSSPTTATTSQSEAAKRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L3T9</accession>
    <name>A0A3B5L3T9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L3T9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004506"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004562.1">
        <property type="protein sequence ID" value="ENSXCOP00000004506.1"/>
        <property type="gene ID" value="ENSXCOG00000003562.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177559"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="67" mass="7317" checksum="2D2E20BC76941AB5" modified="2018-12-05" version="1">MRDCLAHEHTTPEKFFIEACDEGTDAVLVIDRVSNEMTLTGRNDIPPSAVTRPICGIMGTLRLVAGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4L5</accession>
    <name>A0A3B5L4L5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L4L5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002704"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002739.1">
        <property type="protein sequence ID" value="ENSXCOP00000002704.1"/>
        <property type="gene ID" value="ENSXCOG00000002149.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162775"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03426">
        <property type="entry name" value="NUDIX_CoAse_Nudt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045121">
        <property type="entry name" value="CoAse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992:SF11">
        <property type="entry name" value="MITOCHONDRIAL COENZYME A DIPHOSPHATASE NUDT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992">
        <property type="entry name" value="NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="7">
        <location>
            <begin position="145"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="321" mass="36143" checksum="299EAFA07E30E269" modified="2018-12-05" version="1">MTLCCSSQLTSFSADSENYQLEGLISETPISKAILKSHYHQPQVFAKTKAPYVFSTFWHCLFNRTSWPLDLSPATIHNMPALNPRISNQFHCRAQTHYHSVYQIRTVHQAVPRVTDTWKDCLSPENENRCRQILQANWKHYDMDKWASILVSLCYDKGGPAFLFTLRSCTLKGKHKGDVSFAGGKGDPSDRDVVATALREAREELGVTIPAERVWGVMKPLRDTSGMMIAPVLANLGPLEELAFKPNPEEVDEIFTLSLSHLSNPQNRGYTNFRTGNKYGYTLPVFCNGKHRVWGLTAVALDHTIKLLIPPYGSITEVFNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4L6</accession>
    <name>A0A3B5L4L6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ALX homeobox protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cartilage homeoprotein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L4L6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007258"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007351.1">
        <property type="protein sequence ID" value="ENSXCOP00000007258.1"/>
        <property type="gene ID" value="ENSXCOG00000005614.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158251"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048593">
        <property type="term" value="P:camera-type eye morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048702">
        <property type="term" value="P:embryonic neurocranium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000093">
        <property type="entry name" value="ALX homeobox protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050649">
        <property type="entry name" value="Paired_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329:SF359">
        <property type="entry name" value="ALX HOMEOBOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7 13 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 13 14" id="KW-0371">Homeobox</keyword>
    <keyword evidence="11 13 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="117"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="17">
        <location>
            <begin position="303"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="13">
        <location>
            <begin position="119"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="99"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="323" mass="35894" checksum="5FC75B019DBF9CB2" modified="2018-12-05" version="1">MMEYIEDKFALKSQAMKASEYYMDQVMESLDSAQYFNKPSPKCAQAFGLQGAEHRSSPCGDQTSYGVPKTDEDSLRSDLARSIDSCCSLRASPVTSGPEKTELEDAGDKCDSNVSSSKKRRHRTTFTSAQLEELEKVFQKTHYPDVYVREQLAMRTELTEARVQVWFQNRRAKWRKRERYGQIQQAKSHFAATYDLSVLPRSDSYTQIPNNLWPSAAPGGSVVSSCMLPRGSPPCVTSYSHSPRAAAASSTAADHGYVGFAGQQNQFGHVSLNNFFGADSLLTPATNGHPAFEAKPEFERRSSSIAVLRMKAKEHAANISWAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6Y7</accession>
    <name>A0A3B5L6Y7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Argininosuccinate synthase</fullName>
            <ecNumber evidence="4">6.3.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Citrulline--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ASS1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-citrulline + L-aspartate + ATP = 2-(N(omega)-L-arginino)succinate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nitrogen metabolism; urea cycle; (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6Y7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003544"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003583.1">
        <property type="protein sequence ID" value="ENSXCOP00000003544.1"/>
        <property type="gene ID" value="ENSXCOG00000002793.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004524"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00113"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00158">
        <property type="reaction ID" value="UER00272"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004055">
        <property type="term" value="F:argininosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000053">
        <property type="term" value="P:argininosuccinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01999">
        <property type="entry name" value="ASS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000019">
        <property type="entry name" value="Argininosuccinate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1260.10:FF:000005">
        <property type="entry name" value="Argininosuccinate synthase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1260.10">
        <property type="entry name" value="Argininosuccinate synthetase, chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.470">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00005">
        <property type="entry name" value="Arg_succ_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048268">
        <property type="entry name" value="Arginosuc_syn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048267">
        <property type="entry name" value="Arginosuc_syn_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001518">
        <property type="entry name" value="Arginosuc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018223">
        <property type="entry name" value="Arginosuc_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023434">
        <property type="entry name" value="Arginosuc_synth_type_1_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024074">
        <property type="entry name" value="AS_cat/multimer_dom_body"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00032">
        <property type="entry name" value="argG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001770">
        <property type="entry name" value="PRK00509.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587:SF2">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20979">
        <property type="entry name" value="Arginosuc_syn_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00764">
        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69864">
        <property type="entry name" value="Argininosuccinate synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00564">
        <property type="entry name" value="ARGININOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00565">
        <property type="entry name" value="ARGININOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0835">Urea cycle</keyword>
    <feature type="domain" description="Arginosuccinate synthase-like N-terminal" evidence="16">
        <location>
            <begin position="6"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Arginosuccinate synthase C-terminal" evidence="17">
        <location>
            <begin position="178"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF20979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="443" mass="50333" checksum="6B0D6D3104542ACF" modified="2018-12-05" version="1">MSKGTVVLAYSGGLDTSCILVWLKEQGYDVITYLANIGQDEDFEAARKKAESLGAKKVFIEDLRRDFVEDFIWPSVQANAVYEDRYLLGTAIARPCIARRQVEIARKEGAQFVSHGATGKGNDQIRFELTCYALYPEVKIIAPWRIPEFYNRFRGRTDLMEYAQKHGIPVPVTLRAPWSMDANLMHISYESGILENPKSHAPADLYLMTKNPEDSPTAPDVLEIEFKNGVPVRVMHVKEGKSKETPLDIFSYLNEIGGKHGVGRIDIVENRFIGIKSRGIYETPGSTILLKAHLDIETFTMDKEVRRIKQGLGIKFSELVYNGFWYSPECDFVRHCIAKSQENVEGKVQLSVFKGQVYVLGRESPKSLYNEELVSMDVQGDYDPCDASGFIRINAVRLVSLETKTTCNTCEKMFTNSIKSWYSLFLHRLREHHRLQGFSGNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6Z8</accession>
    <name>A0A3B5L6Z8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">guanylate cyclase</fullName>
            <ecNumber evidence="4">4.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP = 3',5'-cyclic GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13665"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="EC" id="4.6.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.6.1.2" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6Z8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005551"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005617.1">
        <property type="protein sequence ID" value="ENSXCOP00000005551.1"/>
        <property type="gene ID" value="ENSXCOG00000004330.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164853"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004383">
        <property type="term" value="F:guanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001653">
        <property type="term" value="F:peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007168">
        <property type="term" value="P:receptor guanylyl cyclase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050401">
        <property type="entry name" value="Cyclic_nucleotide_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011645">
        <property type="entry name" value="HNOB_dom_associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920">
        <property type="entry name" value="GUANYLYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11920:SF228">
        <property type="entry name" value="RETINAL GUANYLYL CYCLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07701">
        <property type="entry name" value="HNOBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0141">cGMP biosynthesis</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Guanylate cyclase" evidence="15">
        <location>
            <begin position="96"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="142" mass="16121" checksum="7FC7147513967956" modified="2018-12-05" version="1">WQTCWLTATSGWTGCSSHRCSWTSLRGKKANIIDSMLRMLEQYSSNLEDLIRERTDELEVERTKTDKLIGQLLPKSVAQALKKGKPVQPEHYSDCTLYFSDIVGFTTISALSEPIEVVDLLNDLYTMFDAIIATHDVYKVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L819</accession>
    <name>A0A3B5L819_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L819"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003934"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003978.1">
        <property type="protein sequence ID" value="ENSXCOP00000003934.1"/>
        <property type="gene ID" value="ENSXCOG00000003094.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00610000087455"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="230" mass="26553" checksum="DF8BEF37839C4997" modified="2018-12-05" version="1">TTEPLCQPCVYHEDFKVELQVRRPLMPVRLSPEQVGLEMLCLCGQLDLLIRAQMQQSEILDQMLQCLEHLPKPMPQLEDYLDMVGLSAMFPRVEVFLIQGSPVDMLDRPLMDYFFHVAKLNQLLVLSQQLEEDIRHLGSHKYIAHQLSVIYQVISSFRGIQVFSKIKKDIETNFKQMKQSLMAEEGCRHEPQLTAHYISILEITKGLTSLVLSLPEELTEDLHQAFTFVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8P6</accession>
    <name>A0A3B5L8P6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">G protein-coupled receptor 157</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027870146.1">
        <property type="nucleotide sequence ID" value="XM_028014345.1"/>
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        <property type="protein sequence ID" value="ENSXCOP00000006136.1"/>
        <property type="gene ID" value="ENSXCOG00000004737.1"/>
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    <dbReference type="GeneID" id="114142837"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="F:cAMP binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
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        <property type="term" value="P:cell surface receptor signaling pathway"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="GCR1-cAMP_receptor"/>
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        <property type="entry name" value="GPCR_2-like_7TM"/>
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        <property type="entry name" value="GPCR_2_secretin-like"/>
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        <property type="entry name" value="GPCR_cAMP"/>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
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        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF47">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 157"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02001">
        <property type="entry name" value="GCR1CAMPR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00247">
        <property type="entry name" value="GPCRCAMP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="7">
        <location>
            <begin position="13"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="8">
        <location>
            <begin position="27"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="306"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="335" mass="37485" checksum="76A98C66C9031CE0" modified="2018-12-05" version="1">MASGNQTEVYLSERVVVLCTCALSVLGSSLIILTHIIWTDLRTTPRRLLVFLSVTDWLSAVSYAFGVWSVFRSDSTECVAQGAISTFSNTSSFFWTVSIAVYLYVFIVRSSQRVADSLVVLFHLVSWGVPLVITVTAVCLNKIGYDASEVSVGWCWVRIQAPDRVLWMLLTGKIWELLAYLTLPVLYILIKRHIHRAHAALSEYRPILATRPPSQSFSSMADVKLTLIPIIFIVLRIWSTVRFILLLANSPVRQTPVLVTLHGIGNTSQGAANCVMFVFFTQPIRSRLCTTLCCCCSNCRAEAPHLDDTPQRHQARRDSRGEEEKDGASGVQRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8U9</accession>
    <name>A0A3B5L8U9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Synaptopodin 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the synaptopodin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8U9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006257.1">
        <property type="protein sequence ID" value="ENSXCOP00000006186.1"/>
        <property type="gene ID" value="ENSXCOG00000004779.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032233">
        <property type="term" value="P:positive regulation of actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051976">
        <property type="entry name" value="Synaptopodin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24217">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24217:SF9">
        <property type="entry name" value="SYNAPTOPODIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="148"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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        </location>
    </feature>
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    </feature>
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    </feature>
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            <end position="771"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
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            <end position="816"/>
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    </feature>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="961"/>
            <end position="992"/>
        </location>
    </feature>
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        <location>
            <begin position="207"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="235"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="292"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="606"/>
            <end position="633"/>
        </location>
    </feature>
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        <location>
            <begin position="742"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="865"/>
            <end position="884"/>
        </location>
    </feature>
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        <location>
            <begin position="961"/>
            <end position="973"/>
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            <begin position="978"/>
            <end position="991"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038161"/>
        </source>
    </evidence>
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    </evidence>
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            <dbReference type="Ensembl" id="ENSXCOP00000006186.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1040" mass="113557" checksum="EF6AA2CE68F66F69" modified="2018-12-05" version="1">MSLGEKVSSDENLKSTTLHIISPKSEIPREVYISDPQGKVLHRELDSDTEALPRGKLSDDLGGHELVFKHNKEVQRCCTPGEFVELQVSLSDQTLAYGGCTSLGSARGIEEDFSAIEPVHTTSSHHVQCPVREPLGQHGVVVRAPTVEDILQPPDASGTGRSTLSVGGPDVTENIGSQSEEEEGGGPSQPVPASFTVSFEIPSDEATLAEEQEDSESEGDQEKPNKHRAKHARLRRNESLSEKQVKEAKSKCKRIALLLSAAPSNPNNKGVLMFKKHRQRAKKYTLVSYGTGEDEPDYSDEEDEVNGDDKQEIPAGEFSFEAAIDCESNGPFLLNAPGSKSVLTFKLGKSLVEINNNLNNQAEMECLPETKGKGAAMFAQRRQRMDEITAEHEELRRQGLPVEAVQELEKKVTEQSYIQSATEGHGYLDHQQMQQYSTNINGTVQHQTNEIQSSYNNRTAKPFTTENMVATPYSHGASGTNQDSFGQGEQIASRDERISTPAIRTGLLSDSRRKTTGKPMFTFKEAPKVSPNPALLNLLNRSDKKGFESGPEEDYLSLGAEACNFLQSQRLKPKIPPPVAPKPLINPNSPPWSAQIEAANQDMPQFPSPPTPQQPSPKPWHPPQNQTPPPPPRRMFSYAVGQKAASPVNPMATVLNPKLSQGPAFEMPVVKGKGADMFAKRQSRMEKFVVDSETVEANKASRSTSPAASLPNEWKYTPNVRAPPPRAYNPIQSPFYPPAATKQPPASSPSIKAKKKDQEKPIPAPKPLNVIDVMKHQPYKLDSSLFIFGTGAEAAKPPVPKPSSPPNSLLDTSSPPYLATPSVPYKALPTNSYIAPSFPVAARPESVTGGGTGAAPKPKFTANKSSAQISASASSSPKPPTRASTNLPVRQTSRLDKGFKPLTPWEAASRHPLGLVDEAFAFQDLQHALASNVHFAARRKILPEPPVEWKGRICYTTQLKPGSRTWSQSSNQGRAPIPSFVSPSRSPASSPVGLQGYRSLPRQWQPQKYVVEANQRHSVSSSETPTYMSAYPSNTWSWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9M3</accession>
    <name>A0A3B5L9M3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5-hydroxyisourate hydrolase</fullName>
            <shortName evidence="4">HIU hydrolase</shortName>
            <shortName evidence="4">HIUHase</shortName>
            <ecNumber evidence="4">3.5.2.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23736"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
            <dbReference type="ChEBI" id="CHEBI:58639"/>
            <dbReference type="EC" id="3.5.2.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the transthyretin family. 5-hydroxyisourate hydrolase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.17" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027859221.1">
        <property type="nucleotide sequence ID" value="XM_028003420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9M3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004534"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004590.1">
        <property type="protein sequence ID" value="ENSXCOP00000004534.1"/>
        <property type="gene ID" value="ENSXCOG00000003586.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114135816"/>
    <dbReference type="KEGG" id="xco:114135816"/>
    <dbReference type="GeneTree" id="ENSGT00940000153229"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033971">
        <property type="term" value="F:hydroxyisourate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05822">
        <property type="entry name" value="TLP_HIUase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.180">
        <property type="entry name" value="Transthyretin/hydroxyisourate hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014306">
        <property type="entry name" value="Hydroxyisourate_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000895">
        <property type="entry name" value="Transthyretin/HIU_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023416">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036817">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02962">
        <property type="entry name" value="hdxy_isourate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10395:SF13">
        <property type="entry name" value="5-HYDROXYISOURATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10395">
        <property type="entry name" value="URICASE AND TRANSTHYRETIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00576">
        <property type="entry name" value="Transthyretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00189">
        <property type="entry name" value="TRNSTHYRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00095">
        <property type="entry name" value="TR_THY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49472">
        <property type="entry name" value="Transthyretin (synonym: prealbumin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transthyretin/hydroxyisourate hydrolase" evidence="5">
        <location>
            <begin position="4"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="119" mass="13095" checksum="436AA328427366A8" modified="2018-12-05" version="1">MAGAPSSPLTTCVVNTSDGVPGAKIALSLHRLDTNLMIWTMIGVGTTNEDGCCPGLISQEAFVPGMYKLRFETGLYWERLGKTLFYPYVETVFTIQNPAQKFNLPLLMSPFSYSTHRGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9V1</accession>
    <name>A0A3B5L9V1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9V1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006521"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006601.1">
        <property type="protein sequence ID" value="ENSXCOP00000006521.1"/>
        <property type="gene ID" value="ENSXCOG00000005049.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048348"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="253" mass="28230" checksum="3CC12BCD1107E10A" modified="2018-12-05" version="1">MHWRGHVSGLAFSFVFTVSYFTNKFVLSVLKFTYPTLFQGWQTFIGAALLLLSGKLGWVEMSRIPRSAALSWLPGSFLFVGNIYAGSRALSRIVSNRSPQRTGGCDAATATCLVTLRMLSSQFDHSGYVWALCHLFCVGKYAWRVCIYIGRLRLPAGDVTGALEFPSLQSHTFHCGCCASALLGFLLLLATVRLKRGPSLEHFRAWVFLSKVNVTKCPTVSSCFQINRRSFIWYRFLPGHSHAPLSFGFQHGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9Z3</accession>
    <name>A0A3B5L9Z3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 21">4-hydroxyphenylpyruvate dioxygenase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the conversion of 4-hydroxyphenylpyruvic acid to homogentisic acid, one of the steps in tyrosine catabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>3-(4-hydroxyphenyl)pyruvate + O2 = homogentisate + CO2</text>
            <dbReference type="Rhea" id="RHEA:16189"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16169"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:36242"/>
            <dbReference type="EC" id="1.13.11.27"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:16190"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="22">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="22">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 3/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 21">Belongs to the 4HPPD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9Z3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007310"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007404.1">
        <property type="protein sequence ID" value="ENSXCOP00000007310.1"/>
        <property type="gene ID" value="ENSXCOG00000005657.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063474"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003868">
        <property type="term" value="F:4-hydroxyphenylpyruvate dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:L-tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07250">
        <property type="entry name" value="HPPD_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.180.10:FF:000022">
        <property type="entry name" value="4-hydroxyphenylpyruvate dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005956">
        <property type="entry name" value="4OHPhenylPyrv_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041735">
        <property type="entry name" value="4OHPhenylPyrv_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01263">
        <property type="entry name" value="4HPPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959:SF11">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009283">
        <property type="entry name" value="HPP_dOase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15 22" id="KW-0408">Iron</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="domain" description="VOC" evidence="23">
        <location>
            <begin position="80"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRNR" id="PIRNR009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR009283-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="294" mass="33907" checksum="819336DFFFD483CA" modified="2018-12-05" version="1">NRKHDSLTARERGAVVVREPWVEQDGNGKVKYAVIQTYGDTTHTLIEYLEPYKGLFLPGYKQPLFRDPLLAKLPPTGLNFIDHIVGNQPDDQMVPISDWYQKCLMFHRFWSIDDKQIHTQYSALRSIVVTNYEETIKMPINEPAMGKKKSQIQEYVDYNGGPGVQHIALNSSNIIKSIENLRERGMEFLSAPDTYYDTLREKLRTAKIQVKEDLDHLQKLKILVDFDDKGYLLQIFTKPVQDRPTLFLEVIQRNNHFGFGAGNFKSLFEAIEMDQDARGNLTVITPEGRAKAFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAB1</accession>
    <name>A0A3B5LAB1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
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    </dbReference>
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        <property type="entry name" value="PROTOCADHERIN ALPHA-C2-LIKE-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="CADHERIN"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CADHERIN_1"/>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="569"/>
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            <end position="109"/>
        </location>
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            <end position="322"/>
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            <end position="432"/>
        </location>
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    <feature type="domain" description="Cadherin" evidence="13">
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            <begin position="447"/>
            <end position="531"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LB47</accession>
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        <submittedName>
            <fullName evidence="8">DnaJ heat shock protein family (Hsp40) member B12b</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Neoteleostei</taxon>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="170" mass="19449" checksum="1C48BDF9D0B0FC0A" modified="2018-12-05" version="1">MFFGGGYPSSKCHLSLSFNSYLLETFPFRDVFLSHPQGGLAVFVQVMPILILVIVSALSQIMVNSPSYSLSFRPSAGYTQKRLTENLKVPYYVGEQFSKEFTGMNLKNLERSVEDDYISNLRNNCWKEKQQKEGMLYRARYFGDSELYQRAQRARTPSCAKLSEITASLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBT6</accession>
    <name>A0A3B5LBT6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="ENSXCOG00000004177.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="141" mass="16111" checksum="A917EE278D3E18A1" modified="2018-12-05" version="1">MLTEAKSKVIFVLCGLGGDAEEPFFSHSCLPFLVILPSSKGLTSIYLSIYLSIYLSIYLSIYLSIYLSIYLSIYLSIYLSIYLSIYIYLSASLMKMTIVHTVPGHLCATSRTFHHCYSWDSGLQNAQEIKYIWKLIYMKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCY5</accession>
    <name>A0A3B5LCY5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cilia- and flagella-associated protein 206</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
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            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Essential for sperm motility and is involved in the regulation of the beating frequency of motile cilia on the epithelial cells of the respiratory tract. Required for the establishment of radial spokes in sperm flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CFAP206 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCY5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010156.1">
        <property type="protein sequence ID" value="ENSXCOP00000010038.1"/>
        <property type="gene ID" value="ENSXCOG00000007616.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000178243"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003356">
        <property type="term" value="P:regulation of cilium beat frequency"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901317">
        <property type="term" value="P:regulation of flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007288">
        <property type="term" value="P:sperm axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021897">
        <property type="entry name" value="FAP206"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21442">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21442:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="96" mass="10575" checksum="FBB8125D1DE22860" modified="2018-12-05" version="1">LPPLSAGVIKTIICEIVEECVRRGHSFSETLAGFMVKAVVLNPTNGFDVDQTLSEEDVQRLKQVFVTVQLTLNSNLTVGPHVRYDKNLTEVEADCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDU4</accession>
    <name>A0A3B5LDU4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L1 transposable element RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDU4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007856"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007954.1">
        <property type="protein sequence ID" value="ENSXCOP00000007856.1"/>
        <property type="gene ID" value="ENSXCOG00000006035.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160789"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1820">
        <property type="entry name" value="L1 transposable element, RRM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004244">
        <property type="entry name" value="Transposase_22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11505">
        <property type="entry name" value="L1 TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="298"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="347" mass="39741" checksum="21C42F01F6CD869B" modified="2018-12-05" version="1">MSGSNKRKTRGIQQENAKETVVLSKEVSEDSKLAADKAMEAMGANILAELKEVQTNLKKQITDQSGLITKEMSEFRKEMGKRLDDIVADLKDVKNRVEEAEQRITEVEEFNMDVKDVLSHTLQLQEDLQTRLTDLEARSRRNNIRIHGVTEGEEGDNLQKFIENFIKTELSLTDTTLGIQRCHRSLGPKLLSGSNPRSIVIHFLEYTTKELVLRSAWGKKVIQLKGNCVFFDQDFPSDIFAKRKAYNPIRKILKEKGLRFQTMYPSRLSVFYQNGPVIYNSAQDASDDMRKRNMFEAAGKPTEDASQPKPRAFSPEGASGTQRWTRETRVKHIQEKLRAFKRNDNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LE50</accession>
    <name>A0A3B5LE50_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Pyroglutamylated RF-amide peptide receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">AQ27</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">G-protein coupled receptor 103</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Orexigenic neuropeptide QRFP receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">SP9155</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for the orexigenic neuropeptide QRFP. The activity of this receptor is mediated by G proteins that modulate adenylate cyclase activity and intracellular calcium levels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LE50"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007956"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008054.1">
        <property type="protein sequence ID" value="ENSXCOP00000007956.1"/>
        <property type="gene ID" value="ENSXCOG00000006106.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286926"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004983">
        <property type="term" value="F:neuropeptide Y receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15205">
        <property type="entry name" value="7tmA_QRFPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000227">
        <property type="entry name" value="Pyroglutamylated RFamide peptide receptor a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000611">
        <property type="entry name" value="NPY_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF20">
        <property type="entry name" value="PYROGLUTAMYLATED RFAMIDE PEPTIDE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01012">
        <property type="entry name" value="NRPEPTIDEYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 17" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 17" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 17 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="311"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="20">
        <location>
            <begin position="71"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="383"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00079726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00082238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="432" mass="48804" checksum="C5A12308D636C3E2" modified="2018-12-05" version="1">MAAASTDSGQGSSKITPEVLREMLQFYNLSRQEFINTYNIQPLVYIPELPYSAKTTFVIMYVVIFVLALAGNSLVIYIVLKKRAIQTATDIFICSLAVSDLLITFFCIPFTLLQNISSEWFGGVLVCKTVPFVQTTAIVTGILTMTCIAIERYQGIVFPLKMRRQYSSKRAYKMLGLVWIASVIVGSPMLFVQQLEVSVKYDFLYDHYHVCCQESWSSLTYRQAYTTFIMVALFLLPLAAMLFLYTRIAIELWIRKRVGDASVLNTMNHREIAVKMMVTIVLLFTICWAPFHTVHMLFEYNDLEKKYDGVTLNMIIAIVQAIGFFNSFNNPIVYAFMNENFKKSCVSTLSHCLRKPNHQGAATVAPRLSVQFIKPQSREAFVNADEDNSSEQHSAERGASCSSHRESSLGIIGEKISTIQTELPPNSSSQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LF03</accession>
    <name>A0A3B5LF03_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Myosin tail domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LF03"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006439"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006519.1">
        <property type="protein sequence ID" value="ENSXCOP00000006439.1"/>
        <property type="gene ID" value="ENSXCOG00000004990.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164626"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000002">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349">
        <property type="entry name" value="CINGULIN-LIKE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349:SF6">
        <property type="entry name" value="MYOSIN-6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="9" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="7" id="KW-0518">Myosin</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin tail" evidence="12">
        <location>
            <begin position="2"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="198"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="218"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="254" mass="29313" checksum="7A84CEE62B1F11A4" modified="2018-12-05" version="1">MAEIEELRAALEQTERSRKVAEQELVDASERVTLLHSQNTSLINTKKKLEADFVQVQGEVEDAIQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEVTVKDLQHRLEEAENLALKGGKKQLQKLEARVRELEGEVEAEQRRGAEAIKGVRKYERRVKELTYQTEEDKKNIARLQDLVDKLQLKVKSYKRQSEDAEEQANAHLSRYRKVQHELEEAQERADIAESQVNKLRAKSREMGRGKEAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LG63</accession>
    <name>A0A3B5LG63_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Regulator of G protein signaling 7 binding protein a</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RGS7BP/RGS9BP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027890895.1">
        <property type="nucleotide sequence ID" value="XM_028035094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LG63"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006849"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006933.1">
        <property type="protein sequence ID" value="ENSXCOP00000006849.1"/>
        <property type="gene ID" value="ENSXCOG00000005293.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114155282"/>
    <dbReference type="GeneTree" id="ENSGT00940000153725"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026512">
        <property type="entry name" value="RGS7BP/RGS9BP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21029">
        <property type="entry name" value="R-SEVEN BINDING PROTEIN (R7BP) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0734">Signal transduction inhibitor</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="250" mass="28167" checksum="FEEE39061437137F" modified="2018-12-05" version="1">MSSASNGRKNRPRSTGNIFQIGKPPNRDPQRRESTESTRKAQRAVADCRMIVQEFNTLVALYRELVISIGEITVDCPTLRADMLKTRTKGCEMARAAHQSLSLISGPEDGEIHPEICRLFIQLQCCLEMYITEMLKSVCLLGSLQLHRKGKDSCCPPGIDTKTEDSDIPILEDTSSSPTDCSQLCWLVATDIENIEKDMRDMKNLLSKLRETMPLPLKNQDDSSLLNLTPYPLVQQRKRRFFGLCCLVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJC9</accession>
    <name>A0A3B5LJC9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJC9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008082.1">
        <property type="protein sequence ID" value="ENSXCOP00000007984.1"/>
        <property type="gene ID" value="ENSXCOG00000006133.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163520"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050822">
        <property type="entry name" value="Cerebellin_Synaptic_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923:SF102">
        <property type="entry name" value="CEREBELLIN 13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923">
        <property type="entry name" value="CEREBELLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017333497" description="C1q domain-containing protein" evidence="4">
        <location>
            <begin position="18"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="5">
        <location>
            <begin position="70"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="206" mass="22576" checksum="FA1A298754F8B852" modified="2018-12-05" version="1">MTFSVWLLVCFCGLTLGQNVFTQTDVATETGSCFPDMCKFLKEFGAMKAKLEAVETNLKEKSEVLELKKKDATIIAFSAATGGSGHIGPFDTDTTLIYRKLIINVGNAYNQHTGIFSAPVPGIYYFTYFYHAGGSQVVNLSLMKNNEEVVTTYDHKTSHDVADNGGNAALLQLQQGDQVYVQLVENTHIWGGSHGTTFSGYLLHQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKG2</accession>
    <name>A0A3B5LKG2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pentraxin family member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="4">Binds 2 calcium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pentraxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKG2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011145"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011273.1">
        <property type="protein sequence ID" value="ENSXCOP00000011145.1"/>
        <property type="gene ID" value="ENSXCOG00000008364.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160409"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051005">
        <property type="entry name" value="Pentraxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45869">
        <property type="entry name" value="C-REACTIVE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45869:SF2">
        <property type="entry name" value="C-REACTIVE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00895">
        <property type="entry name" value="PENTAXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00159">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51828">
        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0106">Calcium</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017104522" description="Pentraxin family member" evidence="4">
        <location>
            <begin position="23"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Pentraxin (PTX)" evidence="5">
        <location>
            <begin position="16"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="218" mass="24289" checksum="946DA5B4357E04C4" modified="2018-12-05" version="1">ISVIFLCFCCLFHGELFPLVLNFEDRNSVSYALAPLHRMDLDAFTVCMHVLLGVPGVHTVISYSSDGRDNELMVSIDTNAREVGLWIGDEYVNMPHSFSTQDWTNYCVTWSSHSGGVDLWINGKVGEQRHLQSNHRINLTGEFVLGKDQDGVLGISDSDAFVGKMTDVNVWDYVLTTAEIRDQFSCERNSTVVGNVLSWGSSRFKMYGGVQPLAEPNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLC3</accession>
    <name>A0A3B5LLC3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Centrosomal protein 135</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CEP135/TSGA10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLC3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011490"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011622.1">
        <property type="protein sequence ID" value="ENSXCOP00000011490.1"/>
        <property type="gene ID" value="ENSXCOG00000008668.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165649"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22292">
        <property type="entry name" value="cc_Cep135_MBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051877">
        <property type="entry name" value="Centriole_BasalBody_StrucProt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20544">
        <property type="entry name" value="CENTROSOMAL PROTEIN CEP135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20544:SF0">
        <property type="entry name" value="NUCLEOPROTEIN TPR_MLP1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="403"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1037"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="103"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="199"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="270"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="445"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="524"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="608"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1038"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1068"/>
            <end position="1081"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1081" mass="124920" checksum="0DF264949C1A2A14" modified="2018-12-05" version="1">MNSNAERKFLNLRKRLDQLGYRQPLGIESLPLVEKLFSDLVHTTESLRNAKLSAGKTEKESRNVDALLEPYKAENSRLVKENNSMHLQLLKLREEKDNFCRELKLQIRKLDNETSDLKFLNNQYVHKVRCLEKDSRAKAERIQQLQEKNMQAVVQTPGGKKRSIPFRRQRMQVDELAPPSYTSAYPVPQPDDPYVADLLQLADGRIHELQEDIADLKLDLENAQESIKHLSIQVEERDTEVERLNRLLHGGRPFDVISLEAHNVSNEKLIAHLNLQIEYLQEANSALQDRIEGLQQKKKDASTEVANLSLKNLELCEELTQIDHLAQCMEMDKERVLENVDIELQKAKVRECQFAVQKELVLELEAFRSKHGVCGRERSPSRLDAFVKSLEEERDFYRHEAERYKRARGAGGPGSSPSRSPDRGRSPKGKGSVPDPELIFVIKEKDELKAALMDFERQMEDIQKNIQVLSNERDHFKTLFKQVRFKYTSENVRRVKMKFDQMTSERDSLMERLKGAQMSALTDRERDERRILELENTVGSLERERLDLRAQVCLLNEGKEAVEEELRVRSIASVQGAEEVAQRLAEANALRLLQEQMEQSLSDVQRRLSVKINELHVAHEQIEELQERLGELSQQSSKWNEEVGTLQKSICVLDREKDILQDEVDQKTEKLVALQDDLSQKEKTLEDVRLTVKNMDNSLAQLQGALKSREREINSLRRQLDSSQEELPALKRDKEVLLRENRRLQDDLATMTRENQVVHLEMEEALHEKDELNSRVQSYISEVSRIEKLMATKEQENRDLLERFRMAHSGMQEWEQKLHQAEGLNNSIRLELLSLDTERRQLQEIVGQKDKEVQQHMQALQAYEAQVSSLTRGMSQLEEDLYKAQEEKAALISDLASVRELCVKLDSGKELAARHLTSKSMELERITGELEDLRSEIEVLKRQLASERLTVRNLETLLSTNRQKEFQTHLAASERESELRALRDKLALADSKNAESIRDASHLRGKVSQLQTEMDVMKRQLTTERFERERAVQEMRRQGLSFSSLQSSPLQNSSSGSHHTSAEGSVLRSHEHSDKSVGFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNG3</accession>
    <name>A0A3B5LNG3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">trypsin</fullName>
            <ecNumber evidence="8">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNG3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012547"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012698.1">
        <property type="protein sequence ID" value="ENSXCOP00000012547.1"/>
        <property type="gene ID" value="ENSXCOG00000009435.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00910000144271"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000005">
        <property type="entry name" value="Serine protease 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000036">
        <property type="entry name" value="Trypsin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF87">
        <property type="entry name" value="ARGININE ESTERASE-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="33"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="262" mass="29093" checksum="6B20F16D1D5FF909" modified="2018-12-05" version="1">MQELLPFVLHCPELLGLKAFYVLFWQWFQGSDIIHGEKAPANSMQYMVSVQNKWGHVCGGFLVRQDIVITAAHCAEDEPVRVVLGTHKLSKRNMSPVNIEYFCKYPTYKSVGLGDDIMLLKLSRKFPLNNRVKIISLPTSSNANLRDGQICSVAGWGQTGPHHAIVDDLRVVNVSILNPQLCRDIWGDDFPSNVICAGGYNTDKGFCQGDSGGPLVCNGVAVGVVSFNKRANCNYPDVPNVYTDISKYLQWINNIVKTNKCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LP19</accession>
    <name>A0A3B5LP19_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">WW domain binding protein VOPP1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Vesicular, overexpressed in cancer, prosurvival protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Late endosome membrane</location>
            <topology evidence="13">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VOPP1/ECOP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LP19"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011191"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011320.1">
        <property type="protein sequence ID" value="ENSXCOP00000011191.1"/>
        <property type="gene ID" value="ENSXCOG00000008464.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015821"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026229">
        <property type="entry name" value="VOPP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14971">
        <property type="entry name" value="VESICULAR, OVEREXPRESSED IN CANCER, PROSURVIVAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14971:SF2">
        <property type="entry name" value="VESICULAR, OVEREXPRESSED IN CANCER, PROSURVIVAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02068">
        <property type="entry name" value="VOPPROTEIN1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0458">Lysosome</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00035628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="134" mass="15676" checksum="9AC99AE70E225217" modified="2018-12-05" version="1">WYFEERYPAYFVCRSYEDCCGTRCCVRALSIQRLWYFWVLLMMGVLFCCGAGFFIRRRMYPSPLRDEPTFNVSFTRQPVTSPGKALLFSVAMLQTAVVVIPGSTQMMMWSYPPPPSYCNHPPPPYEKIIQNDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LP78</accession>
    <name>A0A3B5LP78_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cystatin-B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Stefin-B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cystatin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LP78"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012540"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012691.1">
        <property type="protein sequence ID" value="ENSXCOP00000012540.1"/>
        <property type="gene ID" value="ENSXCOG00000009439.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154826"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071220">
        <property type="term" value="P:cellular response to bacterial lipoprotein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.10:FF:000001">
        <property type="entry name" value="Cystatin-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018073">
        <property type="entry name" value="Prot_inh_cystat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001713">
        <property type="entry name" value="Prot_inh_stefin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11414:SF21">
        <property type="entry name" value="CYSTATIN 14A, TANDEM DUPLICATE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11414">
        <property type="entry name" value="CYSTATIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00295">
        <property type="entry name" value="STEFINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00287">
        <property type="entry name" value="CYSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="domain" description="Cystatin" evidence="9">
        <location>
            <begin position="4"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="101" mass="11494" checksum="B0318A402F980085" modified="2018-12-05" version="1">MDIKCGGISHETKDADENVQQICDKVRPDVEEKAGKTYDVFTAKNYKTQLVAGTNYFIKVHIGGDDYIHLRVWKKLPCYGGDLKLSDIQVSKSHHDSIDYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSS0</accession>
    <name>A0A3B5LSS0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">BTB (POZ) domain containing 3a</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Adapter protein for the cul3 E3 ubiquitin-protein ligase complex. Promotes the export of zbtb16/plzf from the nucleus to the cytoplasm and targets zbtb16/plzf for ubiquitination and degradation. Up-regulates neurog1 expression and antagonizes zbtb16/plzf, to promote neurogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with cul3. Interacts (via BTB domain) with zbtb16/plzf.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSS0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014152"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014329.1">
        <property type="protein sequence ID" value="ENSXCOP00000014152.1"/>
        <property type="gene ID" value="ENSXCOG00000010723.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167000"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021987">
        <property type="term" value="P:cerebral cortex development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000003">
        <property type="entry name" value="BTB/POZ domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.820:FF:000001">
        <property type="entry name" value="BTB/POZ domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000015">
        <property type="entry name" value="BTB/POZ domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.820">
        <property type="entry name" value="PHR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012983">
        <property type="entry name" value="PHR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038648">
        <property type="entry name" value="PHR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45774">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45774:SF2">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08005">
        <property type="entry name" value="PHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="8">
        <location>
            <begin position="84"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="486" mass="53794" checksum="97C6594CFE0C98A8" modified="2018-12-05" version="1">RRPLTSLQSYSSARPRTPPGSSWSSPRRSGSCSAPAGRGSSPSSPFPAPPECGGRNPTTATHRRIRAGLTRELNSVMFNNEMMADVHFVVGPPGGTQRVPGHKYVLAVGSSVFHAMFYGELAEDTEEIRIPDVEPPSFLAMLKYIYCDEIDLCADTVLATLYAAKKYIVPHLARACVNFLETSLSAKNACVLLSQSCLFEEPDLTQRCWEVIDAQAELALRSEGFCDIDAQTLESILRRETLNAKEMVVFEAALSWAEAECHRRDLPPSIDNKRLVLGKAIYLIRIPAMALEDFANGAAQSGMLTLNETNDIFLWFTASRKPELLFCSKPRKGLAPQCCHRFQSCAYRSNQWRYRGRCDSIQFAVDRRVFIAGFGLYGSSCGSAEYSAKMELKRQGVPMAQRVIKFFSDGSSSTFPVWFDYPVQIEPDTFYTASVVLDGNELSYFGQEGMTEVQSGKVTFQFQCSSDSTNGTGVQGGQIPELIFYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTW1</accession>
    <name>A0A3B5LTW1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTW1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011259"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011390.1">
        <property type="protein sequence ID" value="ENSXCOP00000011259.1"/>
        <property type="gene ID" value="ENSXCOG00000008506.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168692"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF127">
        <property type="entry name" value="BLOODY FINGERS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="160"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="339"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="192"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="279"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="279"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="306"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="369" mass="42317" checksum="51EC6E5B79572FCC" modified="2018-12-05" version="1">MPKALGKPESKEDKKSEKAYPCNKCKLIFFWMSDLQIHSLYHCKGQELDVENGGRKISRDGSLEKNKQPTFTPYRCGLCGDRFERLATLKEHHVTHQTQEEIDEMNKEYQKPIKRVMPPNTSRRGSNPNGKLHPCKHCHRVFNHSSSLSRHMRYHKGTMHTCVFCGRHFPQRCDLRRHVIMYHKTEGLKLLHSNSQSGPLSNSADDEKQANNPEDNIKGSSDNEQTSTEQTGKAGRVNYKCQECGKKFGLLCVYQRHLRYHKKEPTKSLKSLADIKKNSSPEVHLQDHLDPEEPDDGQKSPGRGNTVHEELKKDNLEDIEEDAMDQKQNEKGSSSEALYECTECTQTFSCLDTFLEHQASHDSEVKAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUL5</accession>
    <name>A0A3B5LUL5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Syntaxin binding protein 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUL5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011519"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011651.1">
        <property type="protein sequence ID" value="ENSXCOP00000011519.1"/>
        <property type="gene ID" value="ENSXCOG00000008697.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:syntaxin-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.60:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.830.10:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
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        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
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    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="561" mass="63661" checksum="EF7ACDA33E8E1209" modified="2018-12-05" version="1">HKACLQIVNTLNKIIVEDINKRREPLPSMEAIYLITPSDESVEGLIEDFRDPQSPRYRAAHVFFTDTIPDSLFGLLTKSRASKAMKALTEIHIAFLPYESQVFSLDIVDAFQDFYSPFKADVKNNMLERCAEQIATLCATLKEYPGVRYRGEYKDCAVLAQMLQEKLDGYKADDPTMGEGPDKCRTQLVILDRGFDPVSPLLHELTLQAMAYDLLGIENDVYSYETSGMGETRMKEVVLDEDDDLWLTLRHKHIAEVSTAVTRSLKEFSASKKMNTGEKTTMKELSQMLKKMPQYQKELSKYSTHLHLAEDCMNRYQGTVDKLCRVEQDLAMGTDAEGEKIKDPMRLIVPILLDANVNVSDKIRIILLYIFMKNGVTEENLCKLLQHANIPPEDSDIISNMAHLGVPIVSEGTTKKPKKPDRKERVSEQTYQLSRWTPLIKDLIEDAIEDKLDPKQYPYISQRQVSAKASAPSSARYGNWHKNRGPTEMKTGPRIIVFIIGGMAYSEMRCVYEVTQANGKWEALIGSTHILTPPRYLKDLQHPDFLDPNALPEGVEESPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUM0</accession>
    <name>A0A3B5LUM0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUM0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015023.1">
        <property type="protein sequence ID" value="ENSXCOP00000014837.1"/>
        <property type="gene ID" value="ENSXCOG00000011242.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271953"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="65" mass="7234" checksum="330DE51E24AE5511" modified="2018-12-05" version="1">MGLRTFWNNYKVLIVMGTSLGLIHLGWYNLKTNPALHNREEYIPEPGIVAYVSPPAATPPPAKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVE3</accession>
    <name>A0A3B5LVE3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Plasmalemma vesicle associated protein b</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVE3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011799"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011935.1">
        <property type="protein sequence ID" value="ENSXCOP00000011799.1"/>
        <property type="gene ID" value="ENSXCOG00000008920.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006166"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002693">
        <property type="term" value="P:positive regulation of cellular extravasation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043114">
        <property type="term" value="P:regulation of vascular permeability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009538">
        <property type="entry name" value="PV-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21687">
        <property type="entry name" value="PLASMALEMMA VESICLE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21687:SF6">
        <property type="entry name" value="PLASMALEMMA VESICLE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06637">
        <property type="entry name" value="PV-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="149"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="222"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="311" mass="35615" checksum="E9BD452C41FD970F" modified="2018-12-05" version="1">MYSSSCSRVKLGPEARQPLYRNKGKSCGYYMRIVFFFSSLIQSLIIVSLVLLLIYGQPEKSAEEERVNKLELEFNKLSTDNMKLTKEKVELGAKLAACSAEKTALQKEMAKIDFLVTQTFHLNPADNAKCQELIQNETALVKSKDSQMVQNLLSQRDNAIRDRDNAIRDRENVFRDRDTLQEEKKCKEDFVQALSGIQTVTTKFLNKIDNLFPHSLTFHLTCKSQKEQLETIKSNCTNLSRDVENKFQPYLDHVGEKVQWRSSQLEVQNSDLTSKLQQCEDKYKTAVAKATMDLDEQQKLHDQQVGSGAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVR6</accession>
    <name>A0A3B5LVR6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NHP2-like protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">High mobility group-like nuclear protein 2 homolog 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">U4/U6.U5 small nuclear ribonucleoprotein SNU13</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">U4/U6.U5 tri-snRNP 15.5 kDa protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Common component of the spliceosome and rRNA processing machinery.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Involved in pre-mRNA splicing as component of the spliceosome. Binds to the 5'-stem-loop of U4 snRNA and thereby contributes to spliceosome assembly. The protein undergoes a conformational change upon RNA-binding. Core component of box C/D small nucleolar ribonucleoprotein (snoRNP) complexes that function in methylation of multiple sites on ribosomal RNAs (rRNAs) and messenger RNAs (mRNAs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
            <location evidence="1 14">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVR6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011914"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012050.1">
        <property type="protein sequence ID" value="ENSXCOP00000011914.1"/>
        <property type="gene ID" value="ENSXCOG00000009018.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074840"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034512">
        <property type="term" value="F:box C/D sno(s)RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21104">
        <property type="entry name" value="SNU13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000002">
        <property type="entry name" value="NHP2-like protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002415">
        <property type="entry name" value="H/ACA_rnp_Nhp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00883">
        <property type="entry name" value="NUCLEARHMG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="15">
        <location>
            <begin position="24"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="130" mass="14318" checksum="87FA07AE3581E66D" modified="2018-12-05" version="1">MYLTEPQVNPKAYPLADATLTKTILDLVQQASNYKQLRKGANEATKTLNRGIAEFIVMAADAEPLEIILHLPLLCEDKNVPYVFVRSKQALGRACGVSRPVIATSVTIKEGSQLKPQIQSVQMAIERLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LW68</accession>
    <name>A0A3B5LW68_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LW68"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016208"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016414.1">
        <property type="protein sequence ID" value="ENSXCOP00000016208.1"/>
        <property type="gene ID" value="ENSXCOG00000012248.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234607"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035770">
        <property type="term" value="C:ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000963">
        <property type="term" value="P:mitochondrial RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044528">
        <property type="term" value="P:regulation of mitochondrial mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013579">
        <property type="entry name" value="FAST_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010622">
        <property type="entry name" value="FAST_Leu-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013584">
        <property type="entry name" value="RAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF9">
        <property type="entry name" value="FAST KINASE DOMAIN-CONTAINING PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06743">
        <property type="entry name" value="FAST_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08368">
        <property type="entry name" value="FAST_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08373">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00952">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAP"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RAP" evidence="3">
        <location>
            <begin position="600"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="669" mass="75926" checksum="73BAC38ED2514788" modified="2018-12-05" version="1">MALKFTQNLRLLQFSIHHYFAGLISVRLLYSSSLGRSSCAACLCTSAARCIQTHGCRKLRTDFSSRNVTTTVREPSFFASSSVGIHRDSELRFRLTQLYRPSSDEEKSFHQKLVCCSSSRQVFKLLCSVDVLSDTMAAACLHRVADLEQNGAALRDPSVLEKDAIRALCFQLERDSERLTEAGLVSALLACTRLFLDPWSTLMVRLVTESQKRLDGEQMAVGELCMLGQAMLAMEGPGSAMLEQVMGQIQKQEPDEWNLLDLVAVYRLLEVCVGEDGKHGDLLNAMHTHAVTVTSRMDPAAVSGVLSSLVALNQKQAMPLVIGLCKQAVRHVPHFSDEELSHVLAALIHFGHSDRYFVDAMQKHVPKMAFTSHPETVSMVTEFFSRRRILSPTVFNAVAENFVYRADSYSTSQVARQIMAFGKLGYLPPNVGQLFRKVETILHTRFSQFQPRMLLNLLHACTLVERFPVNFLSKVFSSYFLQQLQGETYIDLRIERTVLAQLTQLYMTVKLECPIYEGPQLLPKYRVKSFLASNRSLETPVDQQLYNSVKSGLVDLLGDRSFFGSQVLTPYCYTLDVEIKLDEEGYVLPASHTDEVYKRIALCIDGRKRFTTNVRQLLGKEVIKQRHLQLLGYEVVQIPFYEFEKYRSMSKVVEYLHQKIFPHTFRLSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXI9</accession>
    <name>A0A3B5LXI9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXI9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015862"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016059.1">
        <property type="protein sequence ID" value="ENSXCOP00000015862.1"/>
        <property type="gene ID" value="ENSXCOG00000011979.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="89" mass="9693" checksum="167D1D4E40960FEE" modified="2018-12-05" version="1">MTFTGEFARQLEEKEALVSQLTRGKQAFTQQIEEAELCTDSARPLGAAAGGGCISTGQSYETGRGHVFTKINAKNQKWPKSLIFLGARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXP8</accession>
    <name>A0A3B5LXP8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Signal recognition particle receptor subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
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    <dbReference type="RefSeq" id="XP_027854758.1">
        <property type="nucleotide sequence ID" value="XM_027998957.1"/>
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        <property type="protein sequence ID" value="ENSXCOP00000016688.1"/>
        <property type="gene ID" value="ENSXCOG00000012585.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019009">
        <property type="entry name" value="SRP_receptor_beta_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09439">
        <property type="entry name" value="SRPRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="258" mass="28504" checksum="8302079726921F6D" modified="2018-12-05" version="1">MTENPLEPYIESLRRQLEEQDPVVLVGVIVALAVVVITCVFLKYFLTSKTVRSAVLLVGLCDSGKTLLFIRLLSGKFKRTQTSITDSSAPYKAKNDRSCTWTLIDLPGHDSLRSQYLEKFKSAARAIVFVVDSAIFQKEVRDVAEFLYMLLTDPVVCRIAPTLLVICNKQDVAMAKSAKLIQQQLEKELNTLRVTRSAALSAQDGSVGGSVYLGKKGKDFEFSQLPMKVEFLECSARGSKGKDGDADIESLEKSLAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LY68</accession>
    <name>A0A3B5LY68_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Si:ch211-213o11.11</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LY68"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012908.1">
        <property type="protein sequence ID" value="ENSXCOP00000012754.1"/>
        <property type="gene ID" value="ENSXCOG00000009634.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182998"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15220">
        <property type="entry name" value="7tmA_GPR61_GPR62-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752:SF11">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="210"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="56"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="392" mass="44181" checksum="7CBAAE65F31AA226" modified="2018-12-05" version="1">FFSIIMQFYLKVEKTLSPSTSAWAPMPSAPSRQLGTPPNPQTRFRDLTGLLFMVTLNVLALLANTAVMVVVIKVPHLRKFAFVCHLCAVDLLCAILLMPLGIVSSSPYFAEVVFNVLECQIYVFLNVILIAASIFTITAISVERYYYIVHPMRYEVKMTRKLTLAVVVMVWVASAILGFSTVFGWPSYGQKSISAAHCSLHWSHKDHRRIFSALFSVTCFCLPAVVIFAVYCNVYKVARVAAQQHGPLPSWTNTQVNKAALTLMIIVGQFLICWLPYFAFHLHLILNSKPHTPNDLEVVVTWLAYSSFAMNPFFYGLLNRQIREELCKLKRCYSAYPTDPAFSKTRASFAMPSPRTTLDQTGQTGFRIPGQIPEEKTCTNTLVQTVSKKLFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LY78</accession>
    <name>A0A3B5LY78_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="24">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b. Probably involved in O-linked glycosylation of the immunoglobulin A1 (IgA1) hinge region. Involved in O-linked glycosylation of APOC-III, ANGPTL3 and PLTP. It participates in the regulation of HDL-C metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23956"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12788"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:53604"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52424"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="ChEBI" id="CHEBI:87075"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 24">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 24">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="24">Golgi apparatus membrane</location>
            <topology evidence="24">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 24">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="24"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LY78"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016107"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016310.1">
        <property type="protein sequence ID" value="ENSXCOP00000016107.1"/>
        <property type="gene ID" value="ENSXCOG00000012172.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156958"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031101">
        <property type="term" value="P:fin regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030500">
        <property type="term" value="P:regulation of bone mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd23434">
        <property type="entry name" value="beta-trefoil_Ricin_GALNT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000018">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000026">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF49">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18 24" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 24" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="16 24" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 24" id="KW-0430">Lectin</keyword>
    <keyword evidence="20 24" id="KW-0464">Manganese</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="9 24" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ricin B lectin" evidence="25">
        <location>
            <begin position="428"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00050905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00052209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00058945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="558" mass="63616" checksum="104F88E9E612C399" modified="2018-12-05" version="1">MRRKSRVLLCFAVLWVLGIAYYFYSGTAMSRKVGFPFLDCFFRRHGPPQPAPGWMFPPGQSKVRWQDFNQNLYIGATLVRPGQDPYARNKFNQVESDKLQMDRAVPDTRHDHCRSKQWRSDLPASSVVITFHNEARSALLRTVISVLKKSPPHLVKEIILVDDYSDNPEDGALLGKIEKVRILRNNRREGLMRSRVCGADAATAPVLTFLDSHCECNDHWLEPLLERVAEDKTRVVSPIIDVINMDNFQYVGASADLKGGFDWNLVFKWDYMTLEQRRARQGNPIAPIKTPMIAGGLFVMDKEYFERLGKYDMMMDVWGGENLEISFRVWQCGGSLEIIPCSRVGHVFRKQHPYTFPGGSGTVFARNTRRAAEVWMDEYKNFYYAAVPSARNVPYGNIQSRLEMKKRLGCKPFKWYLENVYPELRVPDHQDIAFGALQQGGNCLDTLGHFADGVVGVYECHNAGGNQEWALTKDKSVKHMDLCLTVVDRTAGSLIKLQGCRENDSRQKWEQIESNSKLRHVGSNLCLDSRSARMGGLTVEVCGPNLNQQWKFTLNLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M119</accession>
    <name>A0A3B5M119_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PRELI/MSF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M119"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015301"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015492.1">
        <property type="protein sequence ID" value="ENSXCOP00000015301.1"/>
        <property type="gene ID" value="ENSXCOG00000011593.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168605"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006797">
        <property type="entry name" value="PRELI/MSF1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04707">
        <property type="entry name" value="PRELI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50904">
        <property type="entry name" value="PRELI_MSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRELI/MSF1" evidence="1">
        <location>
            <begin position="3"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="101" mass="11926" checksum="0E1D9302B406CE40" modified="2018-12-05" version="1">MVQKYQSPVRVYKHPFELVMAAYERRFPTCHLIPMFVNSDVVNEETSEDGSTNRIERRCALDVDAPRLLKRVKYTFTLQPLRKTEGKQEDSKVFFYPDLIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M1J3</accession>
    <name>A0A3B5M1J3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Low density lipoprotein receptor b</fullName>
        </submittedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LDLR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M1J3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017948"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018172.1">
        <property type="protein sequence ID" value="ENSXCOP00000017948.1"/>
        <property type="gene ID" value="ENSXCOG00000013515.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154819"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042562">
        <property type="term" value="F:hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000124">
        <property type="entry name" value="Low density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000116">
        <property type="entry name" value="Low-density lipoprotein receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000009">
        <property type="entry name" value="Low-density lipoprotein receptor isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000034">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000241">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000113">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000030">
        <property type="entry name" value="Sortilin related receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000065">
        <property type="entry name" value="Transmembrane protease serine 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051221">
        <property type="entry name" value="LDLR-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722:SF15">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22722">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 16" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="349"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="18">
        <location>
            <begin position="435"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="18">
        <location>
            <begin position="482"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="18">
        <location>
            <begin position="525"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="18">
        <location>
            <begin position="569"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="732"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="733"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="750"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="85"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="124"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="192"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="211"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="231"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="250"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="353"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="840" mass="93124" checksum="094972A3FF436B2D" modified="2018-12-05" version="1">RLDRLPGLCRVACCCEEYAAFTCSSTQFKCGNGRCVTRRWLCDGNDDCGDGSDELTAVCSAKSCMESQFNCGAPLNHCIPKNWHCDGKPDCENRSDERNCTAKECKTDEFRCTNGQCISKAYVCDKDKDCSDGSDEVDCPKPTCHPRTFQCNNSICVPLLWKCDGDRDCLDGSDEWPENCEGKQTLKKPSQCGAHDFECSNGECIHRAWRCDGTPDCSDQSDEFNCSQPSCHDGQFQCGNGVCISEDLKCNSANDCGDNSDEVSCQTDNVCEGPTNFKCGSGECISMDKVCDSNRDCNDQSDEPLECGKNECSTGNHSCSHQCNDLKIGYNCSCPAGYRLKSDNQTCEDIDECEVPDTCSQICINLPGSYKCDCNDGYQIDPVSKTCKAESGNVATLFFTTKHEVRELTVDRSEYTRLIPELKNAVALDLDMLNKKIFWSDRSQKKIFSSNIDMASNSANHNVVIENGIEAPEALVVDWIHGNIYWTDSTLKTISVATTEGNKRKTLINEKLGRPRAITIDPENNFMYWTDWGEEPKIEKCGLNGVGRVALVTDNIEWPNGITLDMVSQRLYWVDAKLHAIFSVDVNGGTRHMIISGEENIHHPFSLTVFEEKVFWTESFRGTVSSANRLTGNDINELVTDLQNPEALVLYHNLKQPISINWCRVGNSINGGCEYLCLPAPLLDESSPKYTCACPDNMVLAADMKKCVGGMVPLHKYSKTFKSIFLTDPPVMSKTSKPATRGTTKAAKMPTTSSSVQPKQTTDKPAGTPQGKGKLIIAALVCGAVLFWRNWHRKNTNTIHFANPVYQKTTEDEVHICRNGSDGFVYPERQIVSMDDMEIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M256</accession>
    <name>A0A3B5M256_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="15">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">YES1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M256"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015656"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015850.1">
        <property type="protein sequence ID" value="ENSXCOP00000015656.1"/>
        <property type="gene ID" value="ENSXCOG00000011823.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154920"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="SH2_Src_family"/>
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        <property type="entry name" value="SH3_Yes"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Tyrosine-protein kinase"/>
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        <property type="entry name" value="Tyrosine-protein kinase"/>
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    <dbReference type="FunFam" id="3.30.200.20:FF:000016">
        <property type="entry name" value="Tyrosine-protein kinase"/>
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        <property type="entry name" value="Tyrosine-protein kinase"/>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
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        <property type="entry name" value="Tyr_kinase_AS"/>
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        <location>
            <begin position="96"/>
            <end position="157"/>
        </location>
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        <location>
            <begin position="163"/>
            <end position="260"/>
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            <begin position="282"/>
            <end position="535"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
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            <begin position="1"/>
            <end position="10"/>
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            <end position="31"/>
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        </location>
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            <position position="310"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00022443"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50001"/>
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            <dbReference type="PROSITE" id="PS50002"/>
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            <dbReference type="PROSITE" id="PS50011"/>
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            <dbReference type="Ensembl" id="ENSXCOP00000015656.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="548" mass="61330" checksum="98AC6E7DB4ED9BBC" modified="2018-12-05" version="1">MGCVKSKENKGPTMKYQTDNSSRSDPNTSTAVPHVGHYGPDPTQTQQNQAPTSSSSSAALNFNHALTPFGGSSSAMTPFGGASTSFSGPMSNSFSGGVTFFVALYDYEARTSDDLTFKKGDRFQIINNTEGDWWEARSINTGKKGYIPSNYVAPADSIQAEEWYFGKMGRKDAERLLLNPGNNRGTFLVRESETTKGAYSLSIRDWDEAKGDNVKHYKIRKLDNGGYYITTRAQFDTLPKLVKHYTEHADGLCHKLTTVCPTVKPQTQGLAKDAWEIPRESLQLEVKLGQGCFGEVWMGTWNGTTKVAIKTLKPGTMSPEAFLQEAQIMKKLRHDKLVPLYAVVSEEPIYIVTEYMSKGSLLDFVKEGDGKFLTLITLVDMAAKIADGMAFIERMNYIHRDLRAANILVGENLVCKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMVNREVLEQVERGYRMPCPPGCPESLHEMMMLCWKKEPDERPTFEYLQSFLEDYFTSTEPQYQPGENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M311</accession>
    <name>A0A3B5M311_9TELE</name>
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        <recommendedName>
            <fullName evidence="13">High mobility group AT-hook 1a</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <text evidence="2">Belongs to the HMGA family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A3B5M311"/>
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        <property type="gene ID" value="ENSXCOG00000013431.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:transcription coregulator activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:positive regulation of macromolecule biosynthetic process"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23341:SF4">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN HMGI-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23341">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEINS HMG-A AND C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00929">
        <property type="entry name" value="ATHOOK"/>
    </dbReference>
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        <property type="entry name" value="HIGHMOBLTYIY"/>
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        <property type="entry name" value="AT_hook"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
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        </location>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="98" mass="11278" checksum="20FC3EFA37A02F0D" modified="2018-12-05" version="1">MSDRGTKEPSPQSSTAQSPEEPQRRGRGRPRKQQQEPVGPPTPKRPRGRPKGSKSKGPRTALKVKTHHNTFIFIYNYIPMTSKDQQHFFFTYQESINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4V3</accession>
    <name>A0A3B5M4V3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4V3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018472"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018704.1">
        <property type="protein sequence ID" value="ENSXCOP00000018472.1"/>
        <property type="gene ID" value="ENSXCOG00000013906.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156532"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070531">
        <property type="term" value="C:BRCA1-A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031436">
        <property type="term" value="C:BRCA1-BARD1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0085020">
        <property type="term" value="P:protein K6-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17720">
        <property type="entry name" value="BRCT_Bard1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BRCT" evidence="3">
        <location>
            <begin position="35"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="145" mass="15765" checksum="98A83BB648190E83" modified="2018-12-05" version="1">SAGVKACLQAGKWLPEAEHEAGEGPQRSRINRCSLLPPLFDGCFFFLLGSFKGTTKDELAKLLQEGGGQLLRRQPKPDSDVTQTLNAAAYHAEPGSDQALCTQYIIYNPQGTYKPAVVRRGKVWSAPSTWIIDCIAAFSLLPVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5R7</accession>
    <name>A0A3B5M5R7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ependymin-like 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ependymin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M5R7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019753.1">
        <property type="protein sequence ID" value="ENSXCOP00000019513.1"/>
        <property type="gene ID" value="ENSXCOG00000014661.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164430"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001299">
        <property type="entry name" value="Ependymin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10697:SF5">
        <property type="entry name" value="EPENDYMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10697">
        <property type="entry name" value="MAMMALIAN EPENDYMIN-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00811">
        <property type="entry name" value="Ependymin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00317">
        <property type="entry name" value="EPENDYMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00026">
        <property type="entry name" value="EPEND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017243659" description="Ependymin-like 1" evidence="2">
        <location>
            <begin position="22"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="24933" checksum="FF5E8F2DFBAA1A89" modified="2018-12-05" version="1">MNSVGLLFCFSLLLVAATSQAPKPCCELLTRVLPFESPHQMATGGLMTTSGAVAYDALGQRLRARHYGSFGNLTVAVDELMLYQQEVMYEINWSAQTCRKLPLDTYFIPMQVPEDASLLGQTILGSSSSWGMGLLTNTWVGQFPNNDSYLLTFTEIGCLPVTLTNYSPKIGWTTISTYNWVIGMSNPEDFFPPAFCYDAKMEKSAKPQTFFSAMESLAMMVQSMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6B4</accession>
    <name>A0A3B5M6B4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6B4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019187.1">
        <property type="protein sequence ID" value="ENSXCOP00000018950.1"/>
        <property type="gene ID" value="ENSXCOG00000014252.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286918"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001480">
        <property type="entry name" value="Si:cabz01071911.3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF13">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="54"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="111"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="208" mass="24320" checksum="D4D39DBE5E61E6E5" modified="2018-12-05" version="1">RRLKRSSWISITVTLNSDDVHSEWNENPGSDLAYFGQSHRDASFLQQEQILDKKSCRLCGVYFIRDCDLMRHMEEAHAGHKAFKCLECHKEFARRDSLALHMRVHTGEKPHRCPFCEKTFTQTSNLRVHMRKHTGEKPYYCDTCGKMVAHSYHLKICSRQTSYTADITGERAFRCFRCGKKFHTISDLNVHMKIHEARKSHALVQVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9C6</accession>
    <name>A0A3B5M9C6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycerate kinase</fullName>
            <ecNumber evidence="3">2.7.1.31</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-glycerate + ATP = (2R)-3-phosphoglycerate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23516"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16659"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.31"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycerate kinase type-2 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.31" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027857818.1">
        <property type="nucleotide sequence ID" value="XM_028002017.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027857819.1">
        <property type="nucleotide sequence ID" value="XM_028002018.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_028002019.1"/>
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        <property type="protein sequence ID" value="ENSXCOP00000017881.1"/>
        <property type="gene ID" value="ENSXCOG00000013471.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114135078"/>
    <dbReference type="KEGG" id="xco:114135078"/>
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    <dbReference type="GeneTree" id="ENSGT00390000014365"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008887">
        <property type="term" value="F:glycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10180:FF:000001">
        <property type="entry name" value="Glycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10180">
        <property type="entry name" value="Glycerate kinase, MOFRL-like N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1480.10">
        <property type="entry name" value="MOFRL domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037035">
        <property type="entry name" value="GK-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038614">
        <property type="entry name" value="GK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007835">
        <property type="entry name" value="MOFRL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025286">
        <property type="entry name" value="MOFRL_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039760">
        <property type="entry name" value="MOFRL_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12227">
        <property type="entry name" value="GLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12227:SF0">
        <property type="entry name" value="GLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13660">
        <property type="entry name" value="DUF4147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05161">
        <property type="entry name" value="MOFRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82544">
        <property type="entry name" value="GckA/TtuD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MOFRL-associated" evidence="10">
        <location>
            <begin position="33"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="MOFRL" evidence="9">
        <location>
            <begin position="399"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="524" mass="55809" checksum="31BAB851C68AD24C" modified="2018-12-05" version="1">MARILSLFRSQPFLALLGRRNPTFCNMSMDSGARETFRAAVEAVQPDTVVRQSIERNGDSVVIDGRRFTLSHNMHLVGFGKAVLGMAAEVERILGDHLVRGVISVPHGMQQTLQQHGKSHLLLKADSRIKVMEGAKHNLPDDDSLKAAEGIKQLATELTEKDLLLVLISGGGSALLPAPVPPISLQEKLDVTRRLAAAGATIQELNKVRRALSLLKGGGLAHHAHPAQVVSLILSDVIGDPLDLIASGPTVTCEVWPEEVLSILERHKLLSSMPASVTQVLARQGPRWKDSNKHSDSPGEALNAVIGSNTVALTCAGRRVRELGFRPVVLSPGVCGDVRLVSRLYGLLARFACSQEEPSPEIAAEVLRLGPEVGVESWDLCRTMQVLGEGRAEGWGATCVLAGGEPTVELTGKGRGGRNQELALRVGLELKGLELPPQGPVFLSGGTDGQDGPTEAAGAVTDAGLYDEAQAQGLDIDGFLSNNDSYTFFSRLSGGERLLVPGLTCTNVMDVHVLLIPPIPIKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9E6</accession>
    <name>A0A3B5M9E6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein 108</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9E6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016384"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016591.1">
        <property type="protein sequence ID" value="ENSXCOP00000016384.1"/>
        <property type="gene ID" value="ENSXCOG00000012375.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000626"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
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        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097484">
        <property type="term" value="P:dendrite extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097106">
        <property type="term" value="P:postsynaptic density organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008090">
        <property type="term" value="P:retrograde axonal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031508">
        <property type="entry name" value="TMEM108"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28673">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28673:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15759">
        <property type="entry name" value="TMEM108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="412" mass="44325" checksum="79D202CDCB1E6A7A" modified="2018-12-05" version="1">MATPHSSSLSHTKPPSPDWHQEGSTSGEWSPRGAPSSKIVLPTAGLPPSIMLDTNTVIPSTVSTDSHVTQRLWPSSETVNNGSSIMSKFESVREDTDERKKPQDLTDSRQLWPSTDPHSSADVDPPRGLKLREQVIGSPELLVPHTIVLREVPGVDEAPTAQLLIKSTEGPATPVPSPSENFTSPVPITVKSTEPSMKSHKPTVISVNSSDTIEEPWVVLGNGTDSPPMEHQQGHVTGLAGLFSNSSSARVEEASTLGNSSKAPSTDSGNFLNRQVPATTSEPAAAGDSLGSTVNPQLSPTTICLNMMDIVWIVLAISVPVSSISVLLTVCCMRRKKKSTNQENNLSYWNNAITMDYFSRHAVELPREIHTLESEHDTCLPPNGDYSGSSVVLVNPFCQETLFINRDKASAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9U3</accession>
    <name>A0A3B5M9U3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Syntaxin 10</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Golgi apparatus</location>
            <location evidence="9">trans-Golgi network membrane</location>
            <topology evidence="9">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9U3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021018"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021276.1">
        <property type="protein sequence ID" value="ENSXCOP00000021018.1"/>
        <property type="gene ID" value="ENSXCOG00000015725.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166436"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21443">
        <property type="entry name" value="SNARE_NTD_STX6_STX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15851">
        <property type="entry name" value="SNARE_Syntaxin6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.90:FF:000004">
        <property type="entry name" value="Syntaxin 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000006">
        <property type="entry name" value="Syntaxin 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015260">
        <property type="entry name" value="Syntaxin-6/10/61_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09177">
        <property type="entry name" value="STX6_10_61_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="155"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="102"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="117"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="248" mass="28417" checksum="771FB126F528A731" modified="2018-12-05" version="1">MFRIVTLVSCVSLVSREVQKALSRARGLFDRWEELLQEGTQVSRDELDWSANELRNCLRAIDWDLEDLSETISIVESNPGKFRLGDNELQERRDFVERTRKSVQEMKDELSSPSAVERAEKKNRQVSEQDRSTGLEAHLVSANSRYIQEQQEQQQLIIQEQDEQLELVSGSIRVLKDMSGRIGDELDEQAVMLGDFGDEMDQTSSRMDSVLKKLEKVSHMTSSKQTHLPAVFLGFGKPFSQNNLFYGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAD2</accession>
    <name>A0A3B5MAD2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAD2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020537"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020787.1">
        <property type="protein sequence ID" value="ENSXCOP00000020537.1"/>
        <property type="gene ID" value="ENSXCOG00000015389.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159451"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47666">
        <property type="entry name" value="PROTEIN VASCULAR ASSOCIATED DEATH 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47666:SF4">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 8B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="55"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="191"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="289" mass="32993" checksum="E869B7A230E250F5" modified="2018-12-05" version="1">MVLVRQHFLSCYWTMKSPALLHHDPSLAYLEQYQLGFQQFSELFSLLEPWAFCASKSTLSLWIFRLIDENQDGLINFKEFYTLYGVSFTNKLKFLFKLHLPPFVFSSDPLLPVKPTGRGKVDLQAYLKQWQNEILKKEETIKDLPRINQAQFIQFSKTLYNIFHGHPEEESLYRAVAHVTSLLLRMEEVGRRLQEPSSPPAEAAQPASTAASVEASSTQEASLKETSDASSSHSSQEGAAEQLDPDWSFSFEQVLASLLNEPVIVGFFERHVHIQTKLGQSVTRQFSNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBT4</accession>
    <name>A0A3B5MBT4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Cholinergic receptor nicotinic alpha 1 subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Upon acetylcholine binding, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Postsynaptic cell membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-1/CHRNA1 sub-subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBT4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018721"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018957.1">
        <property type="protein sequence ID" value="ENSXCOP00000018721.1"/>
        <property type="gene ID" value="ENSXCOG00000014094.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156851"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022848">
        <property type="term" value="F:acetylcholine-gated monoatomic cation-selective channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19064">
        <property type="entry name" value="LGIC_TM_nAChR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000013">
        <property type="entry name" value="Putative acetylcholine receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000016">
        <property type="entry name" value="Putative acetylcholine receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000019">
        <property type="entry name" value="Putative acetylcholine receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002394">
        <property type="entry name" value="Nicotinic_acetylcholine_rcpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00254">
        <property type="entry name" value="NICOTINICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 20" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="21">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="22">
        <location>
            <begin position="213"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00037634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00038312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="25">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="431" mass="49192" checksum="379D088D06E7D0DE" modified="2018-12-05" version="1">KLVKTLFTGYNKVVRPVNHFSDAVEVTVGLQLIQLISVDEVNQIVTTNVRLRQQWKDVNLQWDPDAYGGIKKIRIPSTDIWKPDLVLYNNADGDFAIIHETKVLLEHTGMITWNPPAIYKSYCEIIVLHFPFDLQNCSMKLGTWTYDGTLVIINPDSDRPDLSNFMESGEWVLKDYRSWKHWVYYTCCLDTPYLDITYHFLMLRLPLYFIVNVIIPCMLFSFLTGLVFYLPTDSGEKMTLSISVLLSLTVFLLVIVELIPSTSSAVPLIGKYMLFTMVFVIASIIITVIVINTHHRSPSTHTMPAWIRKVFIETIPNMMFFSTMKRPSQEISQKRLFPTDMDISDISGNPTPASVTYKSPIMKNPDVRSAIEGVKYIAETMKSDEESNNAAEEWKFVAMVLDHILLCVFMAVCIIGTLAVFAGRLIELNML</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MC06</accession>
    <name>A0A3B5MC06_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Proteasome 20S subunit beta 9a</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
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    <sequence length="170" mass="18840" checksum="AA9C0CA00331F8F6" modified="2018-12-05" version="1">MLGEAEPQWLSEEVKTGTTIIAIEFNGGVVLGFYVNLFKISPLHRYRFSSDYFTGVMNKLSPLHDKIYCALSGSAADAQTIAEMVNYQLDVHSMEIGEDPQVRSAATLVKNISYKYKEELSCQQFVVNTLSLAMNRDGSSGGVAYLVTIDQHGTEEKVILGNDLPTFFDQ</sequence>
</entry>
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    </evidence>
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        </source>
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            <dbReference type="PROSITE" id="PS51035"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="207" mass="22526" checksum="DDCF1AFD7E18D35C" modified="2018-12-05" version="1">MSAQTITVTVAYGSTKHSITLTSQEDGTEPTVKDLSDALNEATGVPPASQKLIFKGKSLKDMEASLSSCGVKEGCKLMMIGKRNSPEEEAELKKLKEIEKSVEQTAKKLEAVDGELTGLKNGFLAKDLQAEALSKLDHRVKIAAEQFMKILEQIDAMAIPENFADCRTKKKGLVKAVQDFLAQCDRIEACISDHLSKIQSKNLALAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEX0</accession>
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        <submittedName>
            <fullName evidence="11">Transmembrane and coiled-coil domain family 3</fullName>
        </submittedName>
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        <name evidence="11" type="primary">TMCC3</name>
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        <name type="scientific">Xiphophorus couchianus</name>
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    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the TEX28 family.</text>
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        <property type="entry name" value="CEREBRAL PROTEIN-11-RELATED"/>
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        <property type="entry name" value="BAR/IMD domain-like"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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            <begin position="23"/>
            <end position="488"/>
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    </feature>
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    </feature>
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        </location>
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            <begin position="232"/>
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        </location>
    </feature>
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    </feature>
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            <begin position="277"/>
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        </location>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="488" mass="54213" checksum="12750C0F85ABB41B" modified="2018-12-05" version="1">YFGYLKCLPVLVLNVHLHCCTAERSSNDANTLSIPVPMRRGSSESNLDVVDSDGVDGIGVDFTKGALGIDRLQQKILKVTEQIKVEQEARDKDVAEYLKLVNNADKQQVTRIRQVFEKRNQKSAQTITSLQKKLEQYHRRMKDGETSGKQSQKDSSSCSSKDSASRSKDDSLRDIGSSGRHPALDKIKTIGPGVSLSPPFFFNKPRDIANLIRNKFGSADNIAHLKSSMESGSGLQAEGTSRGLSGSANAVAKPSKYQSDDECSTGTSASADSNGNLAGGSVTGSGGPGRSDSSGRLLEVLDMVREVRDAQAQLAEDMDSMNTQFKRDYSYFTQVMQEERYRYERLEDQLNDLTELHQHETSNLKQELASIEEKVAYQAYERARDIQEVLESCQTRVSKLELQQQQQQQTVQLENTDAKVLLGRLINIMLAIATVLLVCVSTAAKFTAPLLRSRLHMAFTCVGVSLLVMLWKNWEQVEFTLDRMILPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFB8</accession>
    <name>A0A3B5MFB8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zgc:110626</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MFB8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022402"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022676.1">
        <property type="protein sequence ID" value="ENSXCOP00000022402.1"/>
        <property type="gene ID" value="ENSXCOG00000016734.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164012"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF135">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING 3-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="173"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="292" mass="33120" checksum="EDD19E0CF3B9AFF2" modified="2018-12-05" version="1">MLSTIKKIEITYNSINASNTFTNGDIVSGHVSVEVAKDCQISSFYIKFKGKADVLWSERHGQTTHVYHSKDKYFSVRQYFITDPNCKDSNVLPPGIHVYPFSFQFPLQNIPSSFKGENGKIVYLLEAALSRSMRMDSKESTMINFVGRGDLNPVPGLMEPQHESKDKKMKLFTSGTVAMDVNLEKSGFFQGEGLKVKAFIKNDSSREIKPKYCIYRKHSFFASGKRKVHTKDLIKEVGRPIPPSASENVTQVIAIPQDVEPSILNCSIIKVEHRLRVSINIKRIWKAEEMHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGC4</accession>
    <name>A0A3B5MGC4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycoprotein endo-alpha-1,2-mannosidase</fullName>
            <ecNumber evidence="9">3.2.1.130</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-{alpha-Glc-(1-&gt;3)-alpha-Man-(1-&gt;2)-alpha-Man-(1-&gt;2)-alpha-Man-(1-&gt;3)-[alpha-Man-(1-&gt;2)-alpha-Man-(1-&gt;3)-[alpha-Man-(1-&gt;2)-alpha-Man-(1-&gt;6)]-alpha-Man-(1-&gt;6)]-beta-Man-(1-&gt;4)-beta-GlcNAc-(1-&gt;4)-beta-GlcNAc}-L-asparaginyl-[protein] + H2O = alpha-D-glucosyl-(1-&gt;3)-D-mannopyranose + N(4)-{alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlaNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,2)</text>
            <dbReference type="Rhea" id="RHEA:54824"/>
            <dbReference type="Rhea" id="RHEA-COMP:14010"/>
            <dbReference type="Rhea" id="RHEA-COMP:14011"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:52996"/>
            <dbReference type="ChEBI" id="CHEBI:59080"/>
            <dbReference type="ChEBI" id="CHEBI:60627"/>
            <dbReference type="EC" id="3.2.1.130"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 99 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.130" evidence="9"/>
    <dbReference type="RefSeq" id="XP_027857309.1">
        <property type="nucleotide sequence ID" value="XM_028001508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGC4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018649"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018884.1">
        <property type="protein sequence ID" value="ENSXCOP00000018649.1"/>
        <property type="gene ID" value="ENSXCOG00000014040.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114134734"/>
    <dbReference type="KEGG" id="xco:114134734"/>
    <dbReference type="CTD" id="79694"/>
    <dbReference type="GeneTree" id="ENSGT00390000016054"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004569">
        <property type="term" value="F:glycoprotein endo-alpha-1,2-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd11574">
        <property type="entry name" value="GH99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000019">
        <property type="entry name" value="glycoprotein endo-alpha-1,2-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026071">
        <property type="entry name" value="Glyco_Hydrolase_99"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13572">
        <property type="entry name" value="ENDO-ALPHA-1,2-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13572:SF1">
        <property type="entry name" value="GLYCOPROTEIN ENDO-ALPHA-1,2-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16317">
        <property type="entry name" value="Glyco_hydro_99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017448332" description="Glycoprotein endo-alpha-1,2-mannosidase" evidence="13">
        <location>
            <begin position="31"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="47"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="57"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="444" mass="51115" checksum="CED26486C43E5EAF" modified="2018-12-05" version="1">MARFRLKSCIILVSLALLLVIITLVLKALAPEDSPFDSPGEVEININRQTAPQNQRDFNKRPGETKMSDPVESVKDAELGSTLRKFPPPNYYLHAFYYMWYGNPTFDGKYIHWDHPQLPHWDAKVAQEYPQGRHNPPDDIGANYYPSLGPYSSRDPSVLEAHMQQLRTAAIGVIAISWYPPSTNDDNGEPIDEIIPSLLEVAHKYHIKVAFHIEPYKGRDEVNMFTNVKYIIDKYGEHPAFFKYRTNAGKLLPLFYVYDSYLMNSEQWAKLLKHTDSSSIRDTPYDAIFIALLVEEKHKREILTAGFDGLYTYFATNGFTYGSTQRNWVSIKAFCDDNDLIFIPSVGPGYIDTSVRPWNFKNTRNRINGKYYETSLSAALESRPDFISITSFNEWHEGTQIETSVPKTGQTVYLDYLPNKPTIYLEITRKWAAIFGGERQRWQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGT3</accession>
    <name>A0A3B5MGT3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Selenoprotein O</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SELO family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGT3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020326"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020574.1">
        <property type="protein sequence ID" value="ENSXCOP00000020326.1"/>
        <property type="gene ID" value="ENSXCOG00000015236.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005508"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00692">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003846">
        <property type="entry name" value="SelO"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000658">
        <property type="entry name" value="PRK00029.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12153:SF15">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12153">
        <property type="entry name" value="SELENOPROTEIN O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02696">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="508" mass="57053" checksum="99DCAE4FF74B4330" modified="2025-10-08" version="2">MSPFSSSSQTGSSALERLTFNNTALKRLPIDVSAEPGSRTVPGACFSRVGSLHPLLRPRIVALSQPSFSLLGLSAQEVLDDPLAPEYLSGSRLLAGSEPAAHCYCGHQFGLFAGQLGDGAVMYLGEVESDTQGHWEIQVKGAGVTPYSRDGDGRKVLRSSVREFLCSEAMFALGIPTTRAASLVTSDLHIGRDPLNNGERILERCSVVMRLAPSFISAGRHDNRAQLLDYVIESFYPHIQQAHNNRRERNLSFFREVMMRTAKLVAQWQCVGFCHGVLNTDNMSILGLTLDYGPFGFMDRFDPDFVCNASDKRRRYSYQAQPSVCRWNLARLAEALGSELRAADAGAVLDDFMPTYEAFYLCGMRKKLGLVRKEEAEDREVVSDLLRVMHNTGADFTNTFRLLSRSVRNIGHLQVGFYLFYLSLEPCHNSFKELFFFCCWYTRRRLAREFDGTSDLHLVKKERLRVMKSSNPRVVLRNYIAQNAIQAADKGDFSEVRPKLENPKSPKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHX7</accession>
    <name>A0A3B5MHX7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">R-spondin Fu-CRD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHX7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023352"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023632.1">
        <property type="protein sequence ID" value="ENSXCOP00000023352.1"/>
        <property type="gene ID" value="ENSXCOG00000017439.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160937"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051514">
        <property type="entry name" value="R-spondin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043601">
        <property type="entry name" value="Rspo_Fu-CRD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46987">
        <property type="entry name" value="NEUROHYPOPHYSIAL HORMONES, N-TERMINAL DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46987:SF6">
        <property type="entry name" value="R-SPONDIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15913">
        <property type="entry name" value="Furin-like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="R-spondin Fu-CRD" evidence="6">
        <location>
            <begin position="45"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF15913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="304" mass="34640" checksum="F587293458DC5069" modified="2018-12-05" version="1">KALTATHRNFTFLSTLTLCYLLKSSKLRKLTFIDNYSHRETSEDCQSCLECSRDNGCVRCPERLFLFLQRKGMSHHGTCLHSCPTGHFGQRGRDVNRCMKCRSLDCELCFSRDFCTKCKPGFKLHKGKCLIHCPEGTFAHQTDCLEVCPLLLLGEWSEWSVCVRDGATCGFRWGKQTRVRESSQSWKATEKARVCPPQSETQRCRMKKKCPTGEKDPTVKSLLVSTQHSSRGRSTQKQRGGYCPSAVEPCPPNCILYSPKLNLNSSKVCNCTNNLRLLLHQPCLVRFNRTPVRFLRKSGPFKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHY1</accession>
    <name>A0A3B5MHY1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHY1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023357"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023637.1">
        <property type="protein sequence ID" value="ENSXCOP00000023357.1"/>
        <property type="gene ID" value="ENSXCOG00000017443.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111323"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050901">
        <property type="term" value="P:leukocyte tethering or rolling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013836">
        <property type="entry name" value="CD34/Podocalyxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042397">
        <property type="entry name" value="PODXL2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15594">
        <property type="entry name" value="PODOCALYXIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15594:SF1">
        <property type="entry name" value="PODOCALYXIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06365">
        <property type="entry name" value="CD34_antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="30"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="313"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="54"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="362"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="373" mass="41311" checksum="84F20363EDCB0814" modified="2018-12-05" version="1">MCPMFQRMVHLAVPLGLDGAGGHLELMEPEAERRGGLGAGDASQEASGFDGTDTEDREHGDDGGEDQDRRGGGGEDEWERYAAEDRERRGEEDETGREMDERGVSEAVEVNHTTPDLYALIVNFVLIYCFLSEDRSEELEDERKEREGSRFRGIIPLTTDPSHTAGFTATGFDPGLEELRQVGWFKADDSTGNRRTLKNISTVIVQVVCVNWNELTGRGYVVLNMTKNMNCVSVKIQNHPSLVGIQNYSAATSCQSRPSKTRSDYGKLFVVLVIIGSICMVIITSGFIYICWQRRLPGMKSMVEELHSVENGYHDNPTLDVTNDSQAEMREKKPSTNGLTGAGEGGGDDSSRWQAFVNQAATEDDEEEQDTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIN2</accession>
    <name>A0A3B5MIN2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">A kinase (PRKA) anchor protein 11</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000024193"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024484.1">
        <property type="protein sequence ID" value="ENSXCOP00000024193.1"/>
        <property type="gene ID" value="ENSXCOG00000018065.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153313"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051018">
        <property type="term" value="F:protein kinase A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008382">
        <property type="entry name" value="SPHK1-interactor_AKAP_110"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10226">
        <property type="entry name" value="A KINASE ANCHOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10226:SF3">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="382"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="798"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="896"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="936"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="982"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1079"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1147"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1202"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1254"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1323"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1682"/>
            <end position="1715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1799"/>
            <end position="1818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="821"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="896"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="910"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="952"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1041"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1081"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1103"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1208"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1229"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1255"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1333"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1684"/>
            <end position="1694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1696"/>
            <end position="1708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1828" mass="199946" checksum="994E4F4C058C3844" modified="2018-12-05" version="1">SRKCGRSSDFTVCVCVCVCLQTVRDSGAQSAKSLFRSRKELCGVGLELPARDATRLSEVCSTLSSLPAALCELLKSLHAHSLKSDEVLLLKDSRRLAEHRDVQVAAGAAVCVLRHNPSTGVYPQASVASLVGLLGCYIAGVRYVLELQDLQRVFNEPSQPEEDDTNQSVSSIEDDFVTALEHLEEEDTRDNPSAFCHFKKRDVASQTIPAHKRKKELSGSRIIMSSSSKKHSAKHGPDVSVTVQRSSSGVESQWTYCSPGARPASPSTHLSESEESDCSSPSPIIFLDEVGYQRSLLAKLDIPQVPGGPRDRVEDSDSEVSEFFDSFDQFDDLEELSSESCTLTLPLDSISASTAQNKSEAGSSGPTLKYVSRGCSTKSMNPQCFDQPTLPANVKKPTPLKPGSPYSVHPDMSDSPWLAQTPSEENGGPLFSPVSSSAFSPLVETNGPLEYFWKTEEKGEDKSELRKPQELCSLYKTYSDFANNLSKEILGSVCGFQTAVDISDNKNLSCVCHKEFKNPSGYLMKLSDIQETVTVAKLQKKSQSLKDGIQRFASDLVEMSLGSALRDLQKGVSSCTTTLCHLAARLTSSVFQMAFHEIGMRRAYVLKERAINGLAGFLVGEAISGALKEFLTVKKQIFHNTVTQFAADLAEELVFEGIMEVCQFSHPSTPITPSDWAFGHGQAEEEEEEEVVSSYASDLSESVIQEAFIELSQADVAFTSQAAISVSLDNVCYVNAENTSTHTSSIFANKQVLSSSSTSAVPGLSGEEASCTVKNALFTVSGMASCIPVPHAGQALSSFQDPEEASQYKPDSPKVNTKRLTVTSTDSTSSKHSHAYHHETQNSIPVGGSSQQRLFQNFSGNIVDMIVTEACELITTSKMKKGFGDCTDFLTKSIGSRRNSSSAQETADSPSKHRADRERFRYDGCVRQGWTPEFAPPVSFPAGSSNQGKVQCETDPRGRGVDQTHPVMMDTLNVPSAALLGRGRISGIPDEPPPSSGQKSAGTPGTPPSTPQQPSEVSKEKQIKQFSKKLKSKLANEFSPATPPPTPHYHPEPGPSPEDAIPESDKAEFMLSLMRSLSEEAVGDEDVEEEEPVEEEGVSGTKRGVEADCGQQDHRQVSSRRMSNKEALHYAERLACHIVSMATEMDTLGGSEEGGELSKSSEQRRDSVAQFSEQTLNTLWVYAGEVAGQVINNVKTMISSGQRCPYHRTQELRRRSSSDGWRPRHRHQSQPSTDQSRNSRLAKLAEQWSNDLIASVSQSPHSTSSTSSSSGMSSEYPSCESVTDEYAGYLIKVLKKEGGTRELVLDQYASRLAYRSIKLGLAHASRKNKQRPSSSRLLSSKSLPDEWKASGNETLASKSRSESMGLVSGENTECCCRVSDKQSKREYVDLVNFAESLAYNITCDVTRKLHLSSVRLPKSLTDSCLYKKSKLEDMAENIIRNSFSCPLLSKEDKNRHYHSTGSLSDRGYSSGVMQVIEHYARKIVDDTVKMSLASVGHSSWEHSQTRGYDRHSHAQRLSEGSAAVQSGGERTCRYCLSQECPHCTKLSRHPYQPALQRRIRASESHLASERLPSVEIPKIHIDLDHRAAFAEEVVSLALETAKRELSNASLNADSGIGHDGTSFAESLTAEIMTSALSNVCQAANISSPGREQTESTVSQQLSVGEDSLGSWSNLSFEDDHPDDNSSFLHLSDSVNTEDKETEVRESSGKQRQRSTARAALLIINSDFRELGRGLQPATSDPQLRSMLQWAAASVVDVPQIQLNVDRELQQLPAVVQKLQERKWKVADLLHMLLRYQEESLCHSPPSPPPGGTGDTQHPPLFQWLLEHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLB7</accession>
    <name>A0A3B5MLB7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLB7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024663.1">
        <property type="protein sequence ID" value="ENSXCOP00000024370.1"/>
        <property type="gene ID" value="ENSXCOG00000018199.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="98" mass="11072" checksum="3615DAC6DF703382" modified="2018-12-05" version="1">RRGRLWMLAPPAVPRRFPLCILIQICTGDKSSVSPRLTHMCAYGKEMEHGSLRFQTKRREASLPEGICFLFVSQDHPPPPIPRRSVLLSSLLIPSNAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLF1</accession>
    <name>A0A3B5MLF1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Myomesin 1a (skelemin)</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSXCOT00000022228.1">
        <property type="protein sequence ID" value="ENSXCOP00000021961.1"/>
        <property type="gene ID" value="ENSXCOG00000016397.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154982"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000069">
        <property type="entry name" value="Alpha-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000029">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000124">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000134">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000192">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000197">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000222">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002172">
        <property type="entry name" value="Myomesin 1a (skelemin)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000179">
        <property type="entry name" value="Myomesin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF16">
        <property type="entry name" value="MYOMESIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="180"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="296"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="388"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="513"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="613"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="711"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="815"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="906"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="1371"/>
            <end position="1449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1463" mass="164325" checksum="C29B71E4CD6AE229" modified="2018-12-05" version="1">MSGSVQVTQKQQQKLQLHQQLEEKQHYESSYYLSTRSSVSKQAFSTHKTSSHLKTSVKTDKGQQVVKLSPLPKRAKRTYLAMDKDKEVIGYVIPMFRAHDVVRGLMEAQEDDVTEEGINYVAMRNLFVREAREAMEVKVEKKTRSKHVRESAERMEFDRALEEWSEFRKKMHPDRLTHPPEFIVKPRGQTVWEGKTVRLHCTVAGWPKPRIAYKNNVLIDAKAHPEKYTAESTYNMHSLEIKCDFLDTAQYYVSALNVKGEASSAATVVVKGFCPEHGVTFHTSIIDKFEVAFGCEGETLSLGCTVIIFPTVKKYQPEVVWYRNVPLKPSKWVHTHWSGERATLTLTHLNKEDEGMYTLRVNTKSGFDSYSAYVFVKADVELEGVPVAPLDVCCHDANKDYVVVTWKQPAVEGSSPILGYYIDCEVGTHHWAQCNDAPVKYARFPVTGLVEGRSYVFRVRAFNNVGVSRPSRISDPVVAMDPSDRARLRGPLAPWTGVIKFTEEEPTGVIPGEPTDVVVTEATKSYVVLAWKPPEQRGHEGVMYYIEKCIQGTDTWQRVNTGMPVKSPRFAIFDLAEGKSYNFRVRCCNSAGVGEPSVPTGDTIVGDKLLPSAPGNPVATRNTDTSVVVTWGASKDVKHLVGYYIESSVTGTDVWMPCNNKPVKQTFVCHGLTTGSNYVFRVKAVNAAGYSQNSSNSDAVLVQAAIAVPASPTNVNLLEAVKDYMVLGWTAPTDNGGADIRGYFVDYRTVKGNIYGKWHEMNQKALTTTSYKAENLKENVFYQFQVRAMNMAGVSKPSLPTASLECKEWTITIPPPVGLYVMEVRDTSVVVLWEPPVFDGRSPVNGYYLDIKDASAGEEGWKAVHEKTNKGKFMKVTGLTAGTAYVFRVRSQNLVGVGKPSQDLGPILAQTRPTKEIYVDVDDNGVISMIFECSEMKEGSQFVWFKNYQEITDTSRLTIATQGGSRAIFNSLSMEDLGTFSCMVTNTEGISSSYILTEELLDLSHEHKFPIPFKNEMAMELLEKGCVRFWAQMEKFTSACQVEYVFNDVIIEQGKKYTMKFDKSSGLIELFMDSLEVTDEGTFTFNLVDGKAKGTTSLVLIGDFRELQRKSEFERAEWVRKQPHFVEYLGFTVTQECDVLLKCKLGNIKPVTEIIWSKDSIEIAEDDEDAVKIEKKDCDLTFNIGKWVIKQEQKKGRKKPSAKDVLLPPRPKISKQDAGIYEVFLRDERGKDKSAFNLTEAYQAVMNELFRVINSSSDIQIQSSENGIILYSNVTYYDEDLRVGWLHDAKIAASERVKSGVTGEQLWLKINEPTEKDKGKYTMDIFDGKDGVKRVLDLTWQDAFEEFQRLAAAIAERRARVVGGLPDVVAIQEGKSLNLTGNVWGEPAPEVCWVKNDKELVSDDHYKLKFEHGKFASITIAAVTTADSGKYALVVKNKYGKEAGEFTVSVYNPDEESREDKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MML1</accession>
    <name>A0A3B5MML1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MML1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025968.1">
        <property type="protein sequence ID" value="ENSXCOP00000025659.1"/>
        <property type="gene ID" value="ENSXCOG00000019166.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234575"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03590">
        <property type="entry name" value="CLECT_DC-SIGN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033989">
        <property type="entry name" value="CD209-like_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="5">
        <location>
            <begin position="131"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="72"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="261" mass="29707" checksum="7EC4B309E6ADE2FD" modified="2018-12-05" version="1">MYSQLVDEGSYEESRPDVHQSVKDLRRSGPGPYRFATICLATLCAILLISIIAVTAHYKNKPSPGAEATAEMQKENANVSALMALITKLQKENKQLQKEKEELQVNLTSIMTAKAKPTRAPIVCPTDWRLFNDSCYLISKDSKSWTGSRIYCQRQGGHLAIILTAEEQTFLWELLPRGHWNSYWFGITDGETEDEWKWVDDTPLVGGFWEENEPNNHINEDCGYIVKTEVLERVATKSWYDAPCSMVLPYICEKEMTSTTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MN80</accession>
    <name>A0A3B5MN80_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Inositol 1,4,5-trisphosphate receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Inositol 1,4,5-trisphosphate-gated calcium channel that upon inositol 1,4,5-trisphosphate binding transports calcium from the endoplasmic reticulum lumen to cytoplasm. Exists in two states; a long-lived closed state where the channel is essentially 'parked' with only very rare visits to an open state and that ligands facilitate the transition from the 'parked' state into a 'drive' mode represented by periods of bursting activity.</text>
    </comment>
    <comment type="function">
        <text evidence="21">Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="20">Homotetramer. Interacts with CABP1. Interacts with BOK; regulates ITPR2 expression. Interacts with BCL2L10. Interacts with TRPC4. Interacts with CHGA and CHGB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 21">Endoplasmic reticulum membrane</location>
            <topology evidence="1 21">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="21">The ITPR1 structure has a large solenoid CY assembly built around the central helical bundle made of the C-terminal domains from four ITPR1 subunits. The solenoid scaffold includes domains responsible for binding of ligands and regulatory proteins and is connected via an allosteric nexus at the cytosolic-membrane interface to the transmembrane channel assembly. Six transmembrane helices from each subunit form the central ion-conduction pore.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the InsP3 receptor family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000025011"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025315.1">
        <property type="protein sequence ID" value="ENSXCOP00000025011.1"/>
        <property type="gene ID" value="ENSXCOG00000018664.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155071"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070679">
        <property type="term" value="F:inositol 1,4,5 trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005220">
        <property type="term" value="F:inositol 1,4,5-trisphosphate-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000002">
        <property type="entry name" value="Inositol 1,4,5-trisphosphate receptor type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000005">
        <property type="entry name" value="Inositol 1,4,5-trisphosphate receptor type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.30:FF:000001">
        <property type="entry name" value="Inositol 1,4,5-trisphosphate receptor, type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.30">
        <property type="entry name" value="IP3 receptor type 1 binding core, RIH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014821">
        <property type="entry name" value="Ins145_P3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000493">
        <property type="entry name" value="InsP3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013662">
        <property type="entry name" value="RIH_assoc-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000699">
        <property type="entry name" value="RIH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035910">
        <property type="entry name" value="RyR/IP3R_RIH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816:SF2">
        <property type="entry name" value="INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816">
        <property type="entry name" value="MIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08709">
        <property type="entry name" value="Ins145_P3_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08454">
        <property type="entry name" value="RIH_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01365">
        <property type="entry name" value="RYDR_ITPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00779">
        <property type="entry name" value="INSP3RECEPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100909">
        <property type="entry name" value="IP3 receptor type 1 binding core, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 21" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 21" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="5 21" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="17" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9 21" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16 21" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 21" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 21" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 21" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 21" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="2241"/>
            <end position="2259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="2265"/>
            <end position="2282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="2287"/>
            <end position="2305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="2325"/>
            <end position="2352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="2373"/>
            <end position="2395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="2504"/>
            <end position="2527"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="23">
        <location>
            <begin position="110"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="23">
        <location>
            <begin position="229"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="23">
        <location>
            <begin position="292"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="23">
        <location>
            <begin position="362"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1112"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1686"/>
            <end position="1711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1802"/>
            <end position="1836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1852"/>
            <end position="1876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="2660"/>
            <end position="2683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="1807"/>
            <end position="1822"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="1855"/>
            <end position="1866"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00059076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00061937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU368044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="2683" mass="306304" checksum="AA0B3262C092A641" modified="2018-12-05" version="1">MSDKMSSFLHIGDICSLYAEGSTNGFISTLGLVDDRCVVQPDAGDLSNPPKKFRDCLFKLCPMNRYSAQKQFWKAAKPGGNTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGHVIQLLHLKSNKYLTVNKRLPALLEKNAMRVMLDTAGNEGSWFYIQPFYKLRSIGDSVVIGDKVVLNPVNAGQPLHASTHQLVDNPGCNEVNSVNCSTSWKIVLFMKWSDNQERVLKGGDVVRLFHAEQEKFLTCDDHRKKQYVFLRTTGRQSATSATSSKALWEIEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAELDSEQDPLRARLKIVQDKVLYTLISVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSAYKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAGKLEKGTITQNERRAVTKLLEDLVFFVVDIPNNGQDVLEIMVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRHSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLMFFDPPGDLCVSMNKSIPVTQELICTAVLDPANADILIETKLVLSRFEIEGSQLGENSVEVEEDEEEVWLFWNNSDKEMRSKSVREMAQDAKEGQKEDKEVISYYRYQLNLFARMCLDRQYLAINKISIQLDVDLILRCMSDEDLPFDLRASFCRLMLHMHVDRDPQEQVTPVKYARLWSEIPSKISINDYDNDGTTKDNIKERFWQTMEFVENYLRDVVCQNFPFADKEKNKLTSEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHVSTVFPFTKLEKGDGGRGSNVMRSIHGVGELMSQVVLRGGGFLPINTNSDSNGDTVKTQIEPEKQDILVMDTKLKIIEILQFILNVRLDYRISCLLSIFKREFDESTSQNELSISGSVEGPNNIPGALDFENIEEQAEAIFGGSEENTPLDLDDHGGRTFLRVLLHLTMHEYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVDNYKQIKEDLDKLRSIVEKSELWVYKRQGPDEGLDGGEGMPAEPEPQKAESASSKNYRVIKEVLLRLSRLCIQEGLSGRKSRKQQQRLLRNMGAHAVVLELLQIPYEKGEDVRMQDNMKLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAITMQHIFMNNFQLCSEINDRVVQHFVHCIETHGRNVQYLKFLQTIVKAENKFIKKCQDIVMAELVNAGEDVLVFYNDRASFQTLVNMMRSERDRMDENSPLRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHDDCIPEIKIAYINFLNHCYVDTEVEMKEIYTSNHMWKLFDNFLVDICSVCNNTSDRKHADNVLERYVTETVMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQAIFRVYHCYWLGSGQKGSVEACIKVLSDVAKSRAIAIPVDLDNQVNNLFVRSNNIVQRTAISWRLSVRNSHLSARRESVVTVSRDYRNIIERLQDIVSALEDRLRPLVQAELSVLVDVLHRPELLFPENTDSRKKCESGGFICKLIKHTKQLLEENEEKLCIKVLQTLREMMTKDRGYGEKGEALRQILVSRYYGNFHRSSGRRDSLTSFGNGPLSPVGPGRRGPGGLSRGEMTLSEVQCHLDKEGASDLVIDLIMNTTSDRVFQESILLAIALLEGGNTTIQRSFFCRLTDDKNSEKFFRVFYDRMKSAQQEIKATVTVNTSDLGNKRRDEDNQDRDAPVRKKARDSTVSMTEDVKEQLIEASSATKKAFNSYRREADPEDHCSSTDGLPSAGAKNQEEKMSFVVVIMQPILRFLQLLCENHNRDLQNFLRNQNKKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINEDNVALINQTLESLTEYCQGPCHENQNCIATHESNGIDIIIALILNDINPLGKKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMKPKELVEVIKKAYLQGEVEFVDIKEEEDVRDEEEHDAASPRNVGHNIYILAHQLARHNKELSMMLKPGGGSGDGDEALEFYAKHTAQIEIVRQDRTMEEIVFPVPNICEFLTSESKLRVYYTTERDEQGSKINDFFLRAEDLFNEMNWQKKLRAEPVLYWCSRNMSVWSNISFKLALLMNLLVCFFYPLEGVHGGILEPHLSALLWMGFFAALVLVLLMPKPLGILALVAVTILRLIFSVGLEPTLFLLGAFNVCNKIIFLVSFVGNRGTFTRGYKAMVMDFEFLYHLLYLIICCLGVFGHVFFYSLLLFDLVYREETLLNVIKSVTRNGRSIVLTAVLALILVYLFSIVGYIFFKDDFILEVDRIANATQSKETIGASLTGEFLNTGLCQGGLNENCSNSAQQEEFGIDGEDENDMERTCDSLLMCIVTVLSHGLRSGGGVGDVLRKPSKEEPLFAARVIYDLLFFFMVIIIVLNLIFGVIIDTFADLRSEKQKKEEVLKTTCFICGLERDKFDNKTVTFEEHIKEEHNMWHYLYFIVLVKVKDSTEYTGPESYVAEMIKEHNLDWFPRMRAMSLVSSDSEGEQNEIRNLQEKLESTMRLVINLSGQLTELKEQMTEQRKQKQRLGLLGHPPHLNINPQQPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNR6</accession>
    <name>A0A3B5MNR6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Chromatin modification-related protein MEAF6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 9">Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. Component of HBO1 complexes, which specifically mediate acetylation of histone H3 at 'Lys-14' (H3K14ac), and have reduced activity toward histone H4. Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the NuA4 histone acetyltransferase complex. Component of the hbo1 complex. Component of the moz/morf complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
            <location evidence="9">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Chromosome</location>
            <location evidence="9">Centromere</location>
            <location evidence="9">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the EAF6 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNR6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025186"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025491.1">
        <property type="protein sequence ID" value="ENSXCOP00000025186.1"/>
        <property type="gene ID" value="ENSXCOG00000018814.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015257"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070776">
        <property type="term" value="C:MOZ/MORF histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015418">
        <property type="entry name" value="Eaf6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13476">
        <property type="entry name" value="CHROMATIN MODIFICATION-RELATED PROTEIN MEAF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09340">
        <property type="entry name" value="NuA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0137">Centromere</keyword>
    <keyword evidence="3 9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="9" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="106"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="185" mass="21132" checksum="F233E25D41C742A6" modified="2018-12-05" version="1">MAMHAKATPPQIPDTRRELAELVKRKQELAETLVNLERQIYAFEGSYLEDTQMYGNIIRGWDRYLTNQKNSNSKTDRRNRKFKEAERLFSKSSVTSVAAVCALGGIPDHMNEKRETGSGTESDTSPDLQNQENEPSQDDTEDVDSALQDLKPQKAASSSSSSSHHGNHKKRKNKNRHRYRIHSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MP00</accession>
    <name>A0A3B5MP00_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Desmosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plakin or cytolinker family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000021209"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021469.1">
        <property type="protein sequence ID" value="ENSXCOP00000021209.1"/>
        <property type="gene ID" value="ENSXCOG00000015856.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153578"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030057">
        <property type="term" value="C:desmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045104">
        <property type="term" value="P:intermediate filament cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042060">
        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.780:FF:000001">
        <property type="entry name" value="Plectin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1290.10:FF:000002">
        <property type="entry name" value="Plectin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1290.10">
        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041615">
        <property type="entry name" value="Desmoplakin_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043197">
        <property type="entry name" value="Plakin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035915">
        <property type="entry name" value="Plakin_repeat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058847">
        <property type="entry name" value="Plectin_PPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001101">
        <property type="entry name" value="Plectin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055419">
        <property type="entry name" value="Spectrin_PEPL/EVPL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169">
        <property type="entry name" value="ENVOPLAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169:SF7">
        <property type="entry name" value="ENVOPLAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00681">
        <property type="entry name" value="Plectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26346">
        <property type="entry name" value="Plectin_PPL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17902">
        <property type="entry name" value="SH3_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23160">
        <property type="entry name" value="Spectrin_1st_PEPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00250">
        <property type="entry name" value="PLEC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75399">
        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Periplakin/Envoplakin N-terminal" evidence="9">
        <location>
            <begin position="55"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Desmoplakin SH3" evidence="8">
        <location>
            <begin position="407"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Periplakin-like plectin repeat" evidence="10">
        <location>
            <begin position="946"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="domain" description="Periplakin-like plectin repeat" evidence="10">
        <location>
            <begin position="1184"/>
            <end position="1348"/>
        </location>
    </feature>
    <feature type="domain" description="Periplakin-like plectin repeat" evidence="10">
        <location>
            <begin position="1407"/>
            <end position="1550"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="696"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="884"/>
            <end position="928"/>
        </location>
    </feature>
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        <location>
            <begin position="952"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1190"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1250"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1368"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1458"/>
            <end position="1492"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1565"/>
            <end position="1592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009109"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17902"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF23160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF26346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1989" mass="229735" checksum="6A8EC314C3947151" modified="2018-12-05" version="1">MPFHLKCNNDSIVLCKFFDKDEEKQTFAVSWILQSRPVSVSRKDATDLSVVIKRMQTNADQVEKNILRAEELLAADQEGQRKGKKLLHQRENSANLGQVEKLLKDLFLDVGKAKKLKHPQISEIDSENLHDRWVKGCATYRDLYDQGQELKQKIDWGPVLQEKLVKTLSKLEYGPNLYDVEKQIAEHNILHQEIEAYGSQLQPSSTVSPEDYNAFKEKYAQVLESSRLRRKHLASLYEYMQSCTKELVYLSGQQERIINRDWSDRMIDPSGVRTEYEKFKLNGLMAHEEEVNKLQVEGTELVQTNHPGSQTINTMTDRVQLEWQSFLNLCIAQEMHLENVDNYKKFQLDSDTLAESLQKLSSTLDPKSLANKSNPEILLALEGEEPSVKRNEQRVEALRELSGSVAPLKLRRTQPTEPTTAVSLCDWADENRGQAMILRSNSDNKYWELQDSQGKTTSLPGACFEIPPPDPEALETLKRCRIHHPLQFTSFPNPLFLPHAPEDPRVAELNSDIDKINKALARNEKEILNRLRTPLDSRASMQDLDKRQQENEKSALAVRKLESDKSAVQREVDPILAKKPLGPTAAMLPLNLSAADNKIHNINTLIDLYNKKATASMFLEKQLEKTDVTVSWFEQQLAKDGVILDQPDVLQNRTKNTLKDVVSKKEELKKLGKDLDLTKQACSPLQQRLSEYCPDIRRQESQVQLLKNRYANVRNQLQERVALLKQASNKNQEFNSVAKSLDFFLLNLPDNAVKPTDGVGEILTKENSQKVKIVEDIEKKSGDLQRLKDLSKDLQGILNVSTVNMTLCASCECWFIRKHTLHEETRHILWIIKSYYAYEEKDLLNRFAEISAENNQQLSQLGTAKNLTNEEKVSQVAVSQQLQLQSQQKNVEVADGLKKELEEEVNRRSHAENNLETYRKRFLSLKQRRGVERLEEKEVVQYYRDPKLEADLKSLKNRIQDEEFSRSRIQSEIELLNEKIVTMELQLTKVEPKLVTKVLTEYERDPKLDKEAAEIRDEMERIRTELQTRDTETVHVKSELTLLSQLKQKIREKIVKKEVVRLEKDPEMLKAVLTFQGDIAEEELRCRSLNDIIFSTRSQINTLERVIPSIEPKIVTKVVKQVQQAPELIDESHKIRMALEEEKDENVILKKDLTTLQFRFGELELLKPKVEVKEIVNEIYRVDPDTEVELVRLKKALQDLRQNRSDLEDKIDSVTATLTTLRSQKPKVEYKEVTQEVIKEEKSPEVVRELQRLNNQVSRLQVNYDTTLELLTRLRKERDELKSEKSKVETKLLTKEVIKYENDPLLEKEADRLRRDVREEIHSRRSVEECLFELQNQYITLERQKPEEKIVTTEVIRLQKDPKQIVEHDKLNRSLDDEMKARRKLELEVRQLRALIEEKERDLAKMDNRQKKIQTESELRQIKTRILELENSPPPVEEKIIIEEVLKVERDPKLDKLTDGIRSDMEKESNNINRLEREIRNLRLKLEILQKEKSIEKVVYREVVRVEKDPAVEAERDHLREIVAHERNLRRNQEDSLQSINSQINHIKTSRSVTSQEETALITGRDALQREKEDLLRQLKALESERQTTSITFQQQSRLISERNQMARQRSLKSSSEVQRLEKEILNEKDKIHQRETLIAELQNGITSEDHSESHTRETNLSTRITILDPDTGNDLSPYDAYVKGVIDRNHYLQLCQLECDWEEITSTTPDGDITILQDRKSGKQYSVKDALKDGRLTEYDVSRYKEGKMSISEFAFLVTGEKSKPYIPPNSLNNLTAATGDDYFPISGIFDTTTESRMSVRSALTRRLIDPDTALRLLEAQSASGGIVDLGKKDKLSVHKAVENNLIDSGHKYKLLNAQKAFTGVQDPVTKERLAVGQAEQKGYMPKEDAKRYMEAQYLTGGLVDPNKAGRLTIEEALAENLIDTTMANELKDEALHSKELVDPITKEKISYKQAMDRCKRDVTTGLLLLPAASTNATNAPSYSKYRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQA1</accession>
    <name>A0A3B5MQA1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">LY6/PLAUR domain containing 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQA1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023356"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023636.1">
        <property type="protein sequence ID" value="ENSXCOP00000023356.1"/>
        <property type="gene ID" value="ENSXCOG00000017440.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000220"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030550">
        <property type="term" value="F:acetylcholine receptor inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090263">
        <property type="term" value="P:positive regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039457">
        <property type="entry name" value="LYPD6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31171">
        <property type="entry name" value="LY6/PLAUR DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31171:SF0">
        <property type="entry name" value="LY6_PLAUR DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16975">
        <property type="entry name" value="UPAR_LY6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017372662" description="LY6/PLAUR domain containing 6" evidence="9">
        <location>
            <begin position="23"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="19600" checksum="273E9ED439C23CD0" modified="2018-12-05" version="1">MDAWPAVAWILMLMSVSEWTETAESRDFTMKDIIQLHPSTTPHPGGFKCFTCQDAADNYNCNRWAPDVYCPKDTSYCYTLHMMDNHGDSVSVTKRCATLEHCRFAGCVNITHNGFQVCTSCCHGNVCNVLVPRNDSGAVFSSAGPLVGAGSTPRPAALRYITLITASSCWTHLRSQII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQQ2</accession>
    <name>A0A3B5MQQ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="26">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="function">
        <text evidence="22">Pore-forming subunit of a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na+ ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues.</text>
    </comment>
    <comment type="function">
        <text evidence="23">Pore-forming subunit of a voltage-gated sodium channel complex assuming opened or closed conformations in response to the voltage difference across membranes and through which sodium ions selectively pass along their electrochemical gradient. Contributes to neuronal excitability by regulating action potential threshold and propagation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="25">Voltage-gated sodium (Nav) channels consist of an ion-conducting alpha subunit which is functional on its own associated with regulatory beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 26">Cell membrane</location>
            <topology evidence="2 26">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Axon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.4/SCN4A subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="24">Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.6/SCN8A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000026108"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026422.1">
        <property type="protein sequence ID" value="ENSXCOP00000026108.1"/>
        <property type="gene ID" value="ENSXCOG00000018795.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156263"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13433">
        <property type="entry name" value="Na_channel_gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000002">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000001">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000006">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000002">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000004">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000005">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1190:FF:000003">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000003">
        <property type="entry name" value="Voltage-dependent sodium channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1190">
        <property type="entry name" value="iswi atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MR58</accession>
    <name>A0A3B5MR58_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">NADPH oxidase 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>NADPH + 2 O2 = 2 superoxide + NADP(+) + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MR58"/>
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        <property type="gene ID" value="ENSXCOG00000016279.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:NADPH oxidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016175">
        <property type="term" value="F:superoxide-generating NAD(P)H oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
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    </dbReference>
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        <property type="term" value="P:superoxide anion generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NADPH oxidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NADPH oxidase isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyt_b245_heavy_chain"/>
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        <property type="entry name" value="FAD-bd_8"/>
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        <property type="entry name" value="FAD-bd_FR_type"/>
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        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
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        <property type="entry name" value="Fe_red_NAD-bd_6"/>
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        <property type="entry name" value="FNR_nucleotide-bd"/>
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        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
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        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF203">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00466">
        <property type="entry name" value="GP91PHOX"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01169">
        <property type="entry name" value="NADPH_oxidase_subgroup_(NOX)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD_FR"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="51"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="199"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="11">
        <location>
            <begin position="282"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="555" mass="64677" checksum="C0AEF0C148D4FA00" modified="2018-12-05" version="1">MGNWFVNNWFSAIIISVWMGLNIFIFVWFYFTYEHGERFYYTRHLLGSALAWARAPAAVLNFNCMLILLPVCRNLLSLLRGSFVRCSRTMRKQLDKNLTFHKLVAYMIALMTAVHTVAHLLNVEWLNNNDSHDSHDSHESDDSDHKTTYLNPIRKNNIQIPTYFVFTSIAGLTGVIITLALILIITSSMEVIRRNYFEVFWYTHHLFIIFFAGLVIHGIGYIVRRQGEDSYKKHKPLFCHNRTEDWGKIPECPIPQFEGGPAQTWWYVIGPMVIYLCERLLRFIRYMQNVQYRKIVMRPSKVLELQLVKKGFKMDVGQYVFLNCPAISQLEWHPFTMTSAPEEDFFSVHIRSAGDWTDKLIDIMEKLPEGAQGPKMGVDGPFGTASEDVFDYEVSMLVGAGIGVTPFASILKSIWYKFKDNDPKLRTRKIYFYWLCRETNAFEWFADLLKAQENEMEERGMGDFLTYKLFLTGWDQSHVRVSETDVVTGLKQKTHYGRPNWDKEFEQVRNENPTSVVGTFLCGPEALGKVLEKKCGKYSDVDPRKTKFYFNKENF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSC2</accession>
    <name>A0A3B5MSC2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Methionine adenosyltransferase 2 subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 8">Methionine adenosyltransferase II beta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Regulatory subunit of S-adenosylmethionine synthetase 2, an enzyme that catalyzes the formation of S-adenosylmethionine from methionine and ATP. Regulates MAT2A catalytic activity by changing its kinetic properties, increasing its affinity for L-methionine. Can bind NADP (in vitro).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7 8">Heterotrimer; composed of a catalytic MAT2A homodimer that binds one regulatory MAT2B chain. Heterohexamer; composed of a central, catalytic MAT2A homotetramer flanked on either side by a regulatory MAT2B chain. NADP binding increases the affinity for MAT2A.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the dTDP-4-dehydrorhamnose reductase family. MAT2B subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSC2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024096"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024382.1">
        <property type="protein sequence ID" value="ENSXCOP00000024096.1"/>
        <property type="gene ID" value="ENSXCOG00000017987.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006721"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048269">
        <property type="term" value="C:methionine adenosyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048270">
        <property type="term" value="F:methionine adenosyltransferase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05254">
        <property type="entry name" value="dTDP_HR_like_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000357">
        <property type="entry name" value="Methionine adenosyltransferase 2 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005913">
        <property type="entry name" value="dTDP_dehydrorham_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029903">
        <property type="entry name" value="RmlD-like-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10491">
        <property type="entry name" value="DTDP-4-DEHYDRORHAMNOSE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10491:SF4">
        <property type="entry name" value="METHIONINE ADENOSYLTRANSFERASE 2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04321">
        <property type="entry name" value="RmlD_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RmlD-like substrate binding" evidence="9">
        <location>
            <begin position="30"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="329" mass="36692" checksum="6DDD70B2B28EE5D5" modified="2018-12-05" version="1">MSSACAEQRLVFSPGLVQLVQDEVLPQGPRVLVTGATGLLGRAVCKEFQNHDWTVSGTGLRRARPRFLRCDLTDEDAVRRLLQETKPDVLVHCAAERRPDVMERNADAAVSLNVHASTACGALLIYISTDYVFDGRNPPYGEDDCPNPLNLYGRSKLEGEREALRHCPGAVILRVPVLFGEVESVTESAVTSLWQQVQEATEERRALDHCLQRFPTDTRDVAAVCRKLAERARQDPSIRGIFHFSGKEQMTKYEMAVAMAQAFNLPSNHLVPLTEQPAGSAPRPINSQLNCSRLEQLSLSVEPRAFSVAISECLWPFTADKRWRQTVFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSU8</accession>
    <name>A0A3B5MSU8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RUN domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RUNDC1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">May play a role as p53/TP53 inhibitor and thus may have oncogenic activity.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSU8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024286"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024577.1">
        <property type="protein sequence ID" value="ENSXCOP00000024286.1"/>
        <property type="gene ID" value="ENSXCOG00000018134.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007311"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd17683">
        <property type="entry name" value="RUN_RUNDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.900:FF:000012">
        <property type="entry name" value="RUN domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058732">
        <property type="entry name" value="RUNDC1_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047343">
        <property type="entry name" value="RUSC1_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15591">
        <property type="entry name" value="RUN AND SH3 DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15591:SF19">
        <property type="entry name" value="RUN DOMAIN-CONTAINING PROTEIN 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26030">
        <property type="entry name" value="RUNDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00593">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50826">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RUN" evidence="7">
        <location>
            <begin position="367"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="253"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="119"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00055063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00072888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="559" mass="62411" checksum="5A24DD0D796F54FB" modified="2018-12-05" version="1">MSTEELSASDSETVFASAGERWAPVGAVASPEEESGGRPRQRGSSSATDEEMAARLMKLEEEQDLLNSSLLALTSHFAQVQFRLKQIVHAQSEEKERMLAELEEFAFRGCPHVVGCRAQDAKQLENSSEREKRERLEAQREKQKDLIVQLKTQLDDLERFAYQEGSYDSLPQSVVMERQKVIIDELIKKLDVNLNEDIGNLSPEELRQRVDAAIAQIVNPARVKEQLVEQLKTQIRDLEMFINFIQDEVGNPLLSDGGRSQQPKADGANGRAAGMKKKVDPVQAERMRDTGLQLIQKALAVLQIFAASQFGCSAGHVPQNLWPQDCLGRDYGPLLQHLEAAVEKVRVLGSRRHPSVEHVAALGARDELTTAVRKELAVALKDLLAHGLFCPSQTMSLVLAPISCLLPYRPAPLAMHPWELFVTYYESKNGKAFVESPARKLSQSFSLKVGGGPGTVTPKHSLLWAVHSVLKEHGRYKRGPDSEFKALVCMALNEQRLVSWINLLCKSSTLIQPHYQSWSYMAQTGFEGALRILGRISHLRFNLPVDLAVRQLKNIKDAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MU51</accession>
    <name>A0A3B5MU51_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein-L-isoaspartate(D-aspartate) O-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">L-isoaspartyl protein carboxyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Protein L-isoaspartyl/D-aspartyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Protein-beta-aspartate methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PCMT1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[protein]-L-isoaspartate + S-adenosyl-L-methionine = [protein]-L-isoaspartate alpha-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:12705"/>
            <dbReference type="Rhea" id="RHEA-COMP:12143"/>
            <dbReference type="Rhea" id="RHEA-COMP:12144"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90596"/>
            <dbReference type="ChEBI" id="CHEBI:90598"/>
            <dbReference type="EC" id="2.1.1.77"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:12706"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MU51"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024762"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025063.1">
        <property type="protein sequence ID" value="ENSXCOP00000024762.1"/>
        <property type="gene ID" value="ENSXCOG00000018495.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183032"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000027">
        <property type="entry name" value="Protein-L-isoaspartate O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00080">
        <property type="entry name" value="pimt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF7">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE(D-ASPARTATE) O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="221" mass="23940" checksum="8068B156F99D6706" modified="2018-12-05" version="1">MAWKSGGASHAELVNNLRKNGIIKSDKVYEVMLSTDRAHYSRCNPYMDSPQSIGASSPHAYALELLHDQLHEGAKALDVGSGSGILSVCFARMVGPKGKVIGIDHIKELVDDSINNVKKDDPNLITSGRVKLIVGDGRMGHTEEAPYDAIHVGAAAATVPQALLDQLKPGGRLILPVGPAGGNQMLEQYDKMEDGSTKMKPLMGVIYVPLTDRDKQWSRWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUI5</accession>
    <name>A0A3B5MUI5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Lamellipodium</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MUI5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027171"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027504.1">
        <property type="protein sequence ID" value="ENSXCOP00000027171.1"/>
        <property type="gene ID" value="ENSXCOG00000020287.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155360"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030032">
        <property type="term" value="P:lamellipodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01225">
        <property type="entry name" value="PH_Cool_Pix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000016">
        <property type="entry name" value="Rho guanine nucleotide exchange factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046376">
        <property type="entry name" value="PH_Cool_Pix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46026:SF3">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46026">
        <property type="entry name" value="RHO-TYPE GUANINE NUCLEOTIDE EXCHANGE FACTOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16614">
        <property type="entry name" value="RhoGEF67_u2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="6">
        <location>
            <begin position="20"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="5">
        <location>
            <begin position="219"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="322"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="406"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="417"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="520" mass="58502" checksum="473703D38CF75846" modified="2018-12-05" version="1">MENMEKCKKDSFLITLCPLQVLQNILEAESEYSRELQSLLGTYLRPLHPTDRLSSVDIGHIQGNLEEISTFQQMLVQSLDEQTKLPENQQKIGGFFLNLMPEMKAIYVAYCSNHPSAVSVLTQHSDDLREYMESKGASSPGNLTLTVSLSKPFTRMERYPALLKELDRHMEEQHPDQADLSAAMAAFKSLAAECEEVRKRKNLELQILTEPIRNWEGEDIKTLGPVLHMSQVKESNERFLILFPHTLLMLSASLRMSGFIYQGKMPLSGMLISRIEDGENLKNAFEISGGQCERTQIACSSQRDLQDWLDLLTKHTHTTPPQVCKLQDLPSHPATHSRYSESRGASPYNTLPHPSLNGTISSSTPMWGPLEPPSTPKPWTLSCLRPAPPLRPSAALCFKEDVSKSPKNKRPLLPTIRKPERKPSEEDFTARKSTVALEEDAQILKVIEAYCTSAKTRQTLNSTWQGTDLMHNHVLAETSLTVAALPSNLLSSDQSEDSDYDSIWTNQSHRTASFSRKMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUM9</accession>
    <name>A0A3B5MUM9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP synthase subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase g subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MUM9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024957"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025261.1">
        <property type="protein sequence ID" value="ENSXCOP00000024957.1"/>
        <property type="gene ID" value="ENSXCOG00000018634.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234890"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006808">
        <property type="entry name" value="ATP_synth_F0_gsu_mt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04718">
        <property type="entry name" value="ATP-synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="86" mass="9499" checksum="191666971989E37E" modified="2018-12-05" version="1">MAQKVQKLVAKVPTLLGAAVTYSKPRLATFWYYARVELVPPSPAEIPTAIEAAKGLVRSYQTGRLGRTTVKVSSSDLTFFIHLNYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUR7</accession>
    <name>A0A3B5MUR7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cysteine/serine-rich nuclear protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AXUD1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MUR7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027518"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027852.1">
        <property type="protein sequence ID" value="ENSXCOP00000027518.1"/>
        <property type="gene ID" value="ENSXCOG00000020543.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183072"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031972">
        <property type="entry name" value="CSRNP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023260">
        <property type="entry name" value="Cys/Ser-rich_nuc_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13580:SF13">
        <property type="entry name" value="CYSTEINE_SERINE-RICH NUCLEAR PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13580">
        <property type="entry name" value="TGF-BETA INDUCED APOPTOSIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16019">
        <property type="entry name" value="CSRNP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02031">
        <property type="entry name" value="CYSSERRICHNP"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Cysteine/serine-rich nuclear protein N-terminal" evidence="10">
        <location>
            <begin position="58"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="304"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="482"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="304"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="334"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="361"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="377"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="537" mass="59974" checksum="C7B623F51BAFA8B9" modified="2018-12-05" version="1">MSGILKRKLEEEPSSYLSLQGSEDDEVSCSDTLSLACVSLFYSLPLASVQRQSKRLRGRNVHFESVTVYYFSRRQGFTSVPTQGGSTLGMSPHHSGVKRFTLREFAMEQKRSHQNMLRDHLKEEKLNAIKLKLTKNGTVSSMEADTLTLDDISEDDLDVDNTEVDDYFFLQPLTTRRRRALLRASGVRRIDVEEKHELRALRMSREECGCRCRGICDPETCACSLAGIKCQVDRMSFPCGCTKDGCSNGAGRLEFNPVRVRTHFLHTIMKLELEKSREEQYQQTEQQLVTSGNGYHGNTTLIQQQPQQQHNPQSLKMNNPIMHLQSTGDSDSHLDEDEEEEEDEDDDEEEEDEAYDEDGSSVCSGLSDCSTHSLETLDPEDAEEDDEDDEEEEDEEDEEEDEEEDEEEDWDCSLHGLNPPSYSVPLPSVLSYTNSLLGLSGPFHSASSLQPYQLDVSASDTPAFLGETQNAAAPMLPSVESALESEMGAEPQNRFSELGPGLRTNPDCADSQAEQEPPPPPPPMCGEVSRTAASENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVH7</accession>
    <name>A0A3B5MVH7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CA216</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVH7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025624.1">
        <property type="protein sequence ID" value="ENSXCOP00000025317.1"/>
        <property type="gene ID" value="ENSXCOG00000018901.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169005"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027812">
        <property type="entry name" value="DUF4653"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35673">
        <property type="entry name" value="UPF0500 PROTEIN C1ORF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35673:SF1">
        <property type="entry name" value="UPF0500 PROTEIN C1ORF216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15546">
        <property type="entry name" value="DUF4653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="243" mass="27774" checksum="1F4D25F95D6D27D4" modified="2018-12-05" version="1">MSPCSRLPCWSPLEPLPEIETGDDGYGRVPPDGAEEWRTEEEERMMMFDEKKECQRKEEDPDDEWGDSDSESEFSCRSGGSVSSLNVESGGEAGILGGWDRVGVGEPKSSKPEAGPTRTGDVRQRSFSNKPPTGSDLPGERNHGDNQSSDSDGELPELMDAVWTLRDRERFKAQEMEKHQVQLTMYRRLALIRWVRTLQGRVQEQQNRLQSSFDVILTHRKELLKQRHLKYKHQDVCLYFITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVM2</accession>
    <name>A0A3B5MVM2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVM2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027966.1">
        <property type="protein sequence ID" value="ENSXCOP00000027631.1"/>
        <property type="gene ID" value="ENSXCOG00000020616.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000206001"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="59" mass="6886" checksum="C009848CAD8CCEDB" modified="2018-12-05" version="1">MLFDEEMIRKILNTVNPYTRRNYNFRYLIFGLLCTVYKDGATKSLMNLTGTIPVMFSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWB7</accession>
    <name>A0A3B5MWB7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cholesterol 25-hydroxylase</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWB7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023924"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024208.1">
        <property type="protein sequence ID" value="ENSXCOP00000023924.1"/>
        <property type="gene ID" value="ENSXCOG00000017860.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162142"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="6">
        <location>
            <begin position="131"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="269" mass="31250" checksum="261DA56014934F64" modified="2018-12-05" version="1">VTYLTSQTFNTDSLTQQSRIMLLQPVWSFLLGHSSLLRSPFFPVFFSLTVYLMFCLPFLLLDLLSSRWSLVRRYKLQPQSSINCTMVQSCLALTLYNHLIFIFPLTVLNWYLRPVLLPQEAPPLPQMAAQVLVCLLLFDFQSFIWHLIHHKVPWLYRTFHKLHHTYTSTSALTAEYSEAWETLSLGLFAAANSLLLGCHPLTEMLFFIVNIWLSVEDHCGYDLPWATHRLIPFGLYGGARHHDLHHLKSKCNYAPYFTHWDRLAGTLCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWZ0</accession>
    <name>A0A3B5MWZ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Leukocyte receptor cluster member 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Lysophospholipid acyltransferase 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Membrane-bound O-acyltransferase domain-containing protein 7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 1-hexadecanoyl-sn-glycero-3-phosphocholine = 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + CoA</text>
            <dbReference type="Rhea" id="RHEA:35999"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73003"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:36000"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-octadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol) + (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phospho-(1D-myo-inositol) + CoA</text>
            <dbReference type="Rhea" id="RHEA:36835"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:74243"/>
            <dbReference type="ChEBI" id="CHEBI:133606"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:36836"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1-acyl-sn-glycero-3-phospho-(1D-myo-inositol) + (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA = a 1-acyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phospho-(1D-myo-inositol) + CoA</text>
            <dbReference type="Rhea" id="RHEA:37015"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:64771"/>
            <dbReference type="ChEBI" id="CHEBI:75243"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:37016"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 1-acyl-sn-glycero-3-phospho-(1D-myo-inositol) + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + CoA</text>
            <dbReference type="Rhea" id="RHEA:33195"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:64771"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:33196"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the membrane-bound acyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027869418.1">
        <property type="nucleotide sequence ID" value="XM_028013617.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027869419.1">
        <property type="nucleotide sequence ID" value="XM_028013618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWZ0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026032"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026345.1">
        <property type="protein sequence ID" value="ENSXCOP00000026032.1"/>
        <property type="gene ID" value="ENSXCOG00000019451.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114142365"/>
    <dbReference type="KEGG" id="xco:114142365"/>
    <dbReference type="CTD" id="79143"/>
    <dbReference type="GeneTree" id="ENSGT01030000234564"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044233">
        <property type="term" value="C:mitochondria-associated endoplasmic reticulum membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071617">
        <property type="term" value="F:lysophospholipid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF16">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="420"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="445"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00093678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="472" mass="54239" checksum="3C872876200866BF" modified="2018-12-05" version="1">MSPDELVYLGVLAASIPVGFLFRYLSSSVKQVASLFLGLSITIALCHIHTLHSLVTVIGTWIIIKSSWRHAPALSLSWTFLYLLFFRLVTWFNLPQPTPFANAVQLLLTLKMVSLANEVHSFHVEKKRDVSSFAKSPVIGGLSQEPSFYDILSYSYCYVGIMTGPFFRFQTYMDWLKQPNPLALPSWEPCLQRLKMVPVFGVLFVTVNYYFPLSYVRTDEFLDQNIFFRLFYMVAVFFVFRMRFYSAWCGAEAGCISAGLGCYPEKALSKPGGGPTVNYSPDPSTEEKYNFKTIQNIDCYNTDFCVKVRHGMRYWNMTVQWWLHHYIYPNAPFKSYALRAGWTMFISAYWHGLHAGYYLSFLTIPLCIAAESAMESAVRSKLGPTGQNVFDWIHWFLKMRAYDYMCMGFVLLKASDTINYWMSIYFTMHVIAVGCIIVGKVLKGGRRDGKKGEKERINEEMTEHMKTGQKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXQ2</accession>
    <name>A0A3B5MXQ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXQ2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025146.1">
        <property type="protein sequence ID" value="ENSXCOP00000024845.1"/>
        <property type="gene ID" value="ENSXCOG00000018547.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155611"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000276">
        <property type="entry name" value="zinc finger protein 385A isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000293">
        <property type="entry name" value="zinc finger protein 385B isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051845">
        <property type="entry name" value="Znf385"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23067">
        <property type="entry name" value="DOUBLE-STRANDED RNA-BINDING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23067:SF8">
        <property type="entry name" value="ZINC FINGER PROTEIN 385B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="182"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="366"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="228"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="379"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="423" mass="45506" checksum="40206A43BBEB52CA" modified="2018-12-05" version="1">LLFFLSDENATEPFSASGERADLRLWSDGSGANGHTHTFSQGGTYSLCEVCNLQLTSTAQAQLHYNGRSHLRRVKQLQAGQTRQQAAAHITVETTPSLSHLVTATSHDVASRTLPKLQYGARMRILLLQLSSLTEIKGAMDPVQKAVINHTFGVTMVPKKKQVISCSVCQLRFNSDSQAEAHYRGSRHAKKLKSQENKAKAKLSVTPETNGNSSGSEPPPSSPPSALPAPGLSSSSSSSSSSSSSKSCPPSSVAPAAPQTTSSSSQDAPLSAESEEEKAKKLLYCSLCKVAVNSLSQLEAHNAGSKHKTMLEARSGAGPIKAYPRPGAKLKNGSSSALKGSGLQNKTFHCQICDVHVNSEIQLKQHISSRRHKDRVAGKPSKPKYNPYNKQQRDSLTVSVSFQLSAQLTVEKFTNRTERHKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZP5</accession>
    <name>A0A3B5MZP5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the potassium channel HCN family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MZP5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026409.1">
        <property type="protein sequence ID" value="ENSXCOP00000026095.1"/>
        <property type="gene ID" value="ENSXCOG00000019490.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154743"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000031">
        <property type="entry name" value="Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000002">
        <property type="entry name" value="Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000007">
        <property type="entry name" value="Putative potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013621">
        <property type="entry name" value="Ion_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF7">
        <property type="entry name" value="POTASSIUM_SODIUM HYPERPOLARIZATION-ACTIVATED CYCLIC NUCLEOTIDE-GATED CHANNEL 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08412">
        <property type="entry name" value="Ion_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0114">cAMP</keyword>
    <keyword evidence="7" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="19" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="16 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="10" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="6" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="4" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="8 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="240"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="468"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="25">
        <location>
            <begin position="571"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="732"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="23">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="732"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="23">
        <location>
            <begin position="789"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="815"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="907"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="921"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="951"/>
            <end position="1012"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1048" mass="113117" checksum="AB0D18F6B19E2A57" modified="2018-12-05" version="1">MDGVAGGVGTPGSAGSSGGDTLPRRTDGEAKRRSKGSLPSPGYRLSQASLEGEKGSFSADSSSMGGRGRRPSIMSSSPRDSLPFRAAGTPTTPGTLPIALPPPAASSSSGSTHPPPAPRSVGFAASRAALASTSSTGTGVMVVATGAETTTTACNTTAAGTPDMACQSGLGGFGMGLDGEDYSNSNQSTFIQRQFGAMLQPGVNKFSLRMFGSHKAVAMEQERLKSAGAWIIHPYSDFRFYWDLLMLLLMMGNLIILPVGITFFRDENTPSWIIFNVVSDTLFMVDLVLNFRTGIMKEDSTEILLDPRAIRQKYLKTWFLVDFVSSIPVDYIFLMVDSLDSEVYRTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAMVRIVNLIGMMLLLCHWDGCLQFLVPMLQDFPSDCWVSKNLMVNDTWGVQYSYALFKAMSHMLCIGYGAQAPEGMTDVWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADLRQKIHEYYEHRFQGKMFDEENILGELSEPLKEEIVSFNCRSLVANMPLFANADPNFVTAVLTKLRFEVFQPADFIIREGTVGRKMYFIQHGRVGVLTRGNKETKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDSFNEVLEEHPMMRRAFETVAVDRLDRIGNFWIFLTLYSAAAISQLDTTLLHNAIINLSSVPTAAHIPSTPFLPSSSPPKQTSLSSVTPSPTPSSPTTILTQTPRPKPIPSTLSCCSSPPSASPLSTPRPKPVPTPSPRSSSPSPSSTPPPSAVSTPVPLAQPYGPRSSLTSSSPPSSVPTLTPTVSPIPTPTQTTRTRPSTPTQTLTPTSSPSPSGPSFSKSQSPTSCLQPSVSGSPRVSASSKTPKQTSPLNPIQLSAPTSTPLSTNSPTKVTFSVHPVKQTSPVLSHSPTSGSSLSTKQSPSTTSSSATSCPSQSSSNPSSISPSSSTTTASHQQASTTAANQTAHTPSSANSTSPKGGKKDPQLAGTRTDSEELRHQLPANM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N0F2</accession>
    <name>A0A3B5N0F2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphatidylinositol-specific phospholipase C X domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5N0F2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026580"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026904.1">
        <property type="protein sequence ID" value="ENSXCOP00000026580.1"/>
        <property type="gene ID" value="ENSXCOG00000019846.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010118"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08586">
        <property type="entry name" value="PI-PLCc_BcPLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF113">
        <property type="entry name" value="SI:DKEY-266F7.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidylinositol-specific phospholipase C X" evidence="1">
        <location>
            <begin position="42"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="317" mass="36210" checksum="301272A188BD22A5" modified="2018-12-05" version="1">MNRFHFVFRVVGFSFGAIQRPDYDDTPNPEFLNPSWMASIPDDQPLSVVTMPGTHNTMSLYGGVYAECQTWSLASQLRAGIRFLDVRVRHVNGNLTIHHGVSYQWAHFGQVLEGVFDFLHEYPSETVLMRVKEEFSETNNIYGAVVDFIHRYGHWELLWHSRLVPTMAEARGKLIILQDFTGPDLGMRYGSLDIADAWKVPSLLNLEEKWQSVYKHLEAAPSGNMAQIFLTYSSGAGVFAHPRAVAKGINPQLYEYLQAKTDQNQRLGIICMDFPAAPIIQTIINFQLREVVKTNKTDVKRAIFYFRYMAKHFAANN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N0X3</accession>
    <name>A0A3B5N0X3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Wiskott-Aldrich syndrome protein family member</fullName>
            <shortName evidence="7">WASP family protein member</shortName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Binds actin and the Arp2/3 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
            <location evidence="1 7">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SCAR/WAVE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N0X3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026985"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027318.1">
        <property type="protein sequence ID" value="ENSXCOP00000026985.1"/>
        <property type="gene ID" value="ENSXCOG00000020158.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182962"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031209">
        <property type="term" value="C:SCAR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034237">
        <property type="term" value="F:protein kinase A regulatory subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000601">
        <property type="term" value="P:positive regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000012">
        <property type="entry name" value="Wiskott-Aldrich syndrome protein family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.150">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028288">
        <property type="entry name" value="SCAR/WAVE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12902:SF6">
        <property type="entry name" value="ACTIN-BINDING PROTEIN WASF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12902">
        <property type="entry name" value="WASP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00246">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH2" evidence="9">
        <location>
            <begin position="362"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="174"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="233"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="237"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="424" mass="46770" checksum="170B4B6944DD871A" modified="2018-12-05" version="1">MPLVTRNIEPRHVSRQTIPSNIRSELECVTNISLANIIRQLGSLSKYAEDVFGELFIQAGAFATRVNTLGERVDRLQVKVTQLDPKEEEVSLQAITTRKAFRSSLTQDQQLFTRPSLPVPVQDTYTTCNPPPPLNQLSQYREDGKEALKFYTDPSYFFDLWKEKMLQDTKDIMKEKRKHRREKKDNVNPRTLHPRKIKTRKDEWERLKMGQEFVVPKNETFNDGTAAQQRISMLSPTHPPPAPPSMSPPPNSRPNLSPPPAPPPPPPLSGFAPPPPPPPTGFNGIPSPPPPSFPSPPAPPPPPDMGMAPPPPPIPTGGGPPPPPPPPPPPGPPPPPSDYSAPPPLSAPSSAPKPQPAPVSDGRSDLLQAIRQGFNLRKVEAQREQEKSDHFGNDVAAILSRRIAVECSDSEDDSSEFDDEEWSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N1T3</accession>
    <name>A0A3B5N1T3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">E3 ubiquitin-protein ligase ZNRF1</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">RING-type E3 ubiquitin transferase ZNRF1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Zinc/RING finger protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5N1T3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027770"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028105.1">
        <property type="protein sequence ID" value="ENSXCOP00000027770.1"/>
        <property type="gene ID" value="ENSXCOG00000020706.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159278"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16694">
        <property type="entry name" value="mRING-CH-C4HC2H_ZNRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000235">
        <property type="entry name" value="E3 ubiquitin-protein ligase ZNRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051878">
        <property type="entry name" value="ZNRF_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46661:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZNRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46661">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZNRF1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15" id="KW-0458">Lysosome</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="22">
        <location>
            <begin position="165"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="66"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00040227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="208" mass="22055" checksum="FE50A60FF0760AC0" modified="2018-12-05" version="1">MGGKQSTAGRPRGAFPGVSSDDSAVPPSAHFGHYRPSGTMGLRSRSVSSVAGMGIEHSPAVPFGFYTPRGTDSDRAGGGSGGTTAAPHGTGYQDSGGGGHHTDGVLYLGSRGSLADTLPLHIAPRWFSAHSGKICRTNTHLFTPTHKPSWIYYKDDVLARDAGECVICLEELQQGDTIARLPCLCIYHKSCIDSWFEINRSCPEHPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KLE9</accession>
    <name>A0A3B5KLE9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KLE9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000933"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000945.1">
        <property type="protein sequence ID" value="ENSXCOP00000000933.1"/>
        <property type="gene ID" value="ENSXCOG00000000781.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154427"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14845:SF5">
        <property type="entry name" value="BASAL BODY-ORIENTATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14845">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING 166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="92"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="486" mass="57977" checksum="9F958222C2865E5B" modified="2018-12-05" version="1">MAPSKGKKKKGRKTLKDHILIFEKEKKRMAKMYKTSTKLWEMKLKRTEKDLDLEYHRKEHYKLASEIEELRNVLYQAELSEFGTIDYWQKCLKEKGEKIKSLEEQIESLEELAIEECREREKEKKQHFEKFEEEILKRKAIETRIKKKIANMRRQMQVSKEEYRESLRKKEEEYVRLERKTSEREKNHICKIEKMSRLIDDQEKSIVQLENELHDTERKNNWLRKIFTVTFDNLEDMRQLVQLVSKDKLLLAVDKKMMMSALKKKGCEIAVLQKMLTEVTAVAAYLEQAFLDLEKETTENETKNQVTIQASQVELDKLQKIIAMREKEICQVKKLARTIVEKRRDMEVFFHQALDDVRQEIADERKRNANEAYQDYRQKFRDTVAGRIRLPSIHTLHQCPNSTKSLYSDMKAAEKRPHLPGKEVYMSDLTWEQKEKVLTLLFAKMNGDEERSCRKKTGSEIALHVETAPSSEYFMKHQKGFLIQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQ47</accession>
    <name>A0A3B5KQ47_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQ47"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000601.1">
        <property type="protein sequence ID" value="ENSXCOP00000000592.1"/>
        <property type="gene ID" value="ENSXCOG00000000512.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163911"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784:SF3">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 4 MEMBER K-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="7"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="151" mass="17696" checksum="5CF04DFA5EF30A5A" modified="2018-12-05" version="1">MLSVACAQSHQYYFVNTSLTWRDAQRVCRRDYTDLATIESTADVDDFLRTTSNNTGKAWIGLYDDKINSWKWSLNDSSFYSPGEYSFRDWEPNEPNNYYGQQYCVRFYGTAYNSVGRWDDIGCTETIQFVCYNGKIDIYKLLASKIFTIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KR00</accession>
    <name>A0A3B5KR00_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Neurotransmitter-gated ion-channel transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KR00"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002548"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002583.1">
        <property type="protein sequence ID" value="ENSXCOP00000002548.1"/>
        <property type="gene ID" value="ENSXCOG00000002030.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154617"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19051">
        <property type="entry name" value="LGIC_TM_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000007">
        <property type="entry name" value="Neuronal acetylcholine receptor subunit alpha-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000011">
        <property type="entry name" value="neuronal acetylcholine receptor subunit alpha-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="360"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="7">
        <location>
            <begin position="99"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="217"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="392" mass="43338" checksum="279E7BE70589086F" modified="2018-12-05" version="1">QKIPFTSQYLSVSLKMIQLGENLSCRSLRRVIIYRNITLSLGRHPLLCKRHRYLCGCASPTGVPAKRNELYYECCKEPYPDVTFTVTMRRRTLYYGLNLLIPCVLISGLALLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMMIVGMSVVVTVIVLQFHHHDPHGGKMPKWVRVVLLNWCAWFLRMKQPGEERKRPQYKYRHTSQHHSSTSSIEMGTMPSLTVPLSQASCPPCTTGTSNGSMSLYFGSYHPMESPHCPPSSDSGVTLGGRTHGSMGDETEPPGGVCGVGSLGMGVGIPPPEILRILEEVSYIAQRFRDQDKAEAICSEWKFAAAVVDRLCLVAFSLFSIICTFTILMSAPNFIEAVSKDFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRW0</accession>
    <name>A0A3B5KRW0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2 Aida-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Acts as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the AIDA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRW0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002880.1">
        <property type="protein sequence ID" value="ENSXCOP00000002843.1"/>
        <property type="gene ID" value="ENSXCOG00000002258.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016465"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048264">
        <property type="term" value="P:determination of ventral identity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046329">
        <property type="term" value="P:negative regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.360:FF:000001">
        <property type="entry name" value="Axin interactor, dorsalization-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000059">
        <property type="entry name" value="Axin interactor, dorsalization-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.360">
        <property type="entry name" value="Axin interactor, dorsalization-associated protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025939">
        <property type="entry name" value="Aida_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023421">
        <property type="entry name" value="AIDA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036818">
        <property type="entry name" value="AIDA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28654">
        <property type="entry name" value="AXIN INTERACTOR, DORSALIZATION-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28654:SF1">
        <property type="entry name" value="AXIN INTERACTOR, DORSALIZATION-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14186">
        <property type="entry name" value="Aida_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08910">
        <property type="entry name" value="Aida_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109779">
        <property type="entry name" value="Domain from hypothetical 2610208m17rik protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51911">
        <property type="entry name" value="C2_AIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 Aida-type" evidence="6">
        <location>
            <begin position="147"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00059715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="262" mass="29677" checksum="88844B7EF51D197E" modified="2018-12-05" version="1">MSDVNKTVQKWHASFKKGTDFDSWGQLVEAIDEYQQLQKEVQSTNSSDFTEEQKKNLGKIATCLEMRSASLQCTQSKEEFKLEDLKKLELIKNILTYNKEFPFDVQPVPLKILAPGEEENLELEAEDDAAAGAGSTGTFPPRAPTLLPRLPSEPGMTLLTLRIEKIGLKDAGQCIDPYMSISVKVNGVDLNPMQDTPVATRKEDTYIHFSVDIEIQRHVEKLPKAAIFFEFKHYKPKKRFTSTKCFAFMEMDEIKPGPIVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KSG8</accession>
    <name>A0A3B5KSG8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tripartite motif-containing protein 16-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KSG8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000585.1">
        <property type="protein sequence ID" value="ENSXCOP00000000576.1"/>
        <property type="gene ID" value="ENSXCOG00000000505.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286922"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19842">
        <property type="entry name" value="Bbox1_TRIM25-like_C-IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="15"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="9">
        <location>
            <begin position="149"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="10">
        <location>
            <begin position="366"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="197"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="550" mass="63618" checksum="F6B84CB5CB883DF2" modified="2018-12-05" version="1">MEQQAVKLDRETFSCSICLDLLKDPVTTSCGHSYCMNCITKFWDEGEKKENYHCPQCSTTFTQKPDLQKNTMLAALVEQLKKTGLQAAPADHCYAGPEDVACDSCTGRKLRAIKSCLVCLASFCEKHLQPHFDSVAFKKHKLVEPSKNLQENICSKHDEVMKMFCRTDQKCICYLCPVDEHKGHETVSAAAERTERQRELEERRGNIQQRIQDREKDVKLLQQEVEAINRSADKTVEDSEKIFTQLIRLLQKRSSEVKQQIRSQQETVVSRVKDVQEKLEQEITELKRKDAELEQLSHTQDHNQFLLKYPSLPALSESTHSSSINIRPLRHFEDVTAAVSELRDKLQDVLRDSWTNISLMVTEVDVLLSEPEPKSRTGFLRYSCEITLDPNTANTWLRLSEGNRKVTWMNQHQSYSSHPDRFTDWSQILSRESLTGPCYWEVEWRGLVYVAVAYKSISRVGWGNECGFGNNDKSWALYSYQSTFWHNNIPTSISGPVSSRVGVYLDHRAGILSFYNATSQYAEYIYIYIYIYIYSDHICIFYYTNRSFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KUG1</accession>
    <name>A0A3B5KUG1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ArfGAP with FG repeats 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KUG1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002147"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002178.1">
        <property type="protein sequence ID" value="ENSXCOP00000002147.1"/>
        <property type="gene ID" value="ENSXCOG00000001719.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161071"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001675">
        <property type="term" value="P:acrosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007289">
        <property type="term" value="P:spermatid nucleus differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08838">
        <property type="entry name" value="ArfGap_AGFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000005">
        <property type="entry name" value="Arf-GAP domain and FG repeat-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052248">
        <property type="entry name" value="Arf-GAP_FG-repeat_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46134:SF6">
        <property type="entry name" value="ARF-GAP DOMAIN AND FG REPEAT-CONTAINING PROTEIN 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46134">
        <property type="entry name" value="DRONGO, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="7">
        <location>
            <begin position="14"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="127"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="168"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="239" mass="26567" checksum="4154D6B3386B4158" modified="2018-12-05" version="1">MSNRKHRDNQEICSRKVRELAQSGVNKHCFECNQPGVTYTDITVGSFVCTSCSGMLRGLNPPHRVKSISMTTFSQQEVEFLQNHGNEVGRRTWLCVFDPKADGCPDLKDPQKFKEFLQDKYEKKKWHFSKSKNRRDVEGPWSPGVQAVPSSHGPLSSQGPSHNLPPNARSTRPLSQSQLPSWDRAPTISPADMRTDAFTRPGSLSSALGGQSHGPSFPALPRPSGGLWSPSTLEKHRSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KX85</accession>
    <name>A0A3B5KX85_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KX85"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002241"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002272.1">
        <property type="protein sequence ID" value="ENSXCOP00000002241.1"/>
        <property type="gene ID" value="ENSXCOG00000001789.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161394"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE RDL2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086:SF3">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE_RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 1 ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017229633" description="Rhodanese domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="47"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="146" mass="15997" checksum="F902D842DAC058C8" modified="2018-12-05" version="1">MGSLCGNLAVLLLFLSPLMHSHLFARASTSSPPTTLKYEELKALIESNKDLVLIDVRSQGEVAGGRIPGAIHIPIHALDAALRMNPDDFKANYGLVKPPLNVPQLVFYCQAGGRSRMAATTAIRNGYINVRDYAGAYSEWSRKERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXW2</accession>
    <name>A0A3B5KXW2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Vacuole membrane protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027854966.1">
        <property type="nucleotide sequence ID" value="XM_027999165.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027854967.1">
        <property type="nucleotide sequence ID" value="XM_027999166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXW2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000394"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000401.1">
        <property type="protein sequence ID" value="ENSXCOP00000000394.1"/>
        <property type="gene ID" value="ENSXCOG00000000330.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114133342"/>
    <dbReference type="KEGG" id="xco:114133342"/>
    <dbReference type="CTD" id="81671"/>
    <dbReference type="GeneTree" id="ENSGT00390000007230"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="115"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="251"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="405" mass="45731" checksum="6C19104DC401B2B4" modified="2018-12-05" version="1">MAANGASCEQPQKRAGPKDKHNGKSTDLLLRDRRQKDKEERLSLVLWRRPVDTLHYFLLETLVKLKEWTFELWHRRGTVFVFLLLCFLFSITYSTDGSHQTYVQYLEKKFLWCAYWVGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECGSIDFPEPPYPDQIICPKDGGLEDSISIFSIMSKVRLEACMWGAGTAIGELPPYFMARAARQSGTDPDDEDYEEFEEMLEQAEGAQDFVTRAKLGVQHMVQKVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKLFVIITFSKHIVEQMVSLIGSVPTIGPSLQKPFSQYLEAQKNKLHHAGKSGPADENWLSWVFEKVVLVMVCYFVISIINSMAQSYAKRLQQLRHSEEKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYG8</accession>
    <name>A0A3B5KYG8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    </evidence>
    <sequence length="75" mass="8931" checksum="5DAF3350F62539D3" modified="2018-12-05" version="1">MKLMADRNLVKELKRWATEEFNLPPDSLPNESYLKTLCVGTGKSIWTYMIQHVFQQRNVRIMRGNLQWYPFLALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZR6</accession>
    <name>A0A3B5KZR6_9TELE</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="DREV"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="281" mass="32319" checksum="69DE7011C2AFADE3" modified="2018-12-05" version="1">KRRRRIQWSRWYRCCPDLMGESVRPLFVQSHLDTDTKAFLKHSQEKSGWLFTQLYHSLVSSVLSPIVSRTSINGFLGRGSMFVFSADQFQQLLQIGPDWEADRLLDLGAGDGGVTEVMGVHFKQVYATEVSPPMKWHLQRRNYTLLGIDEWQHTGFQYDVISCLNLLDRCDDPLHLLRDIKSSLVPKTGRLVLAAVLPFQPYIEIGGKWERPKEHLKIAGKTWEEQVTNLCREVFKRAGFELEAVTRLPYLCEGDMYNDYYVLDDAVFVLKASEDSGDSPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZX5</accession>
    <name>A0A3B5KZX5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane protein 229B</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <text evidence="2">Belongs to the TMEM229 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZX5"/>
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        <property type="protein sequence ID" value="ENSXCOP00000001114.1"/>
        <property type="gene ID" value="ENSXCOG00000000925.1"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="CmpB_TMEM229"/>
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        <property type="entry name" value="TRANSMEMBRANE PROTEIN 229 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31746:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 229B"/>
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    </dbReference>
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        <property type="entry name" value="ABC_trans_CmpB"/>
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        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="67"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="100"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="133"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="153"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="184"/>
            <end position="194"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00006371"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="194" mass="22755" checksum="02BF6990372F5A20" modified="2018-12-05" version="1">METLDPVPLTALSRWYLYAIHGYFCEVMFTAAWEFVVNCNWKFPGVTSVWALFIYGTCILIVERMYLKLRGRCPVLLRCIIYTLWTYLWEFGTGLLLRQFNACPWDYTEFRYNFMGLITAEYAVPWFCASFTVERFVIRKTLRLRFNEGPEDGWSNHRHDAGGGEGAGETLEGNRQREQRRGSDFNSNGYLKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L082</accession>
    <name>A0A3B5L082_9TELE</name>
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        <submittedName>
            <fullName evidence="2">NDUFA4 mitochondrial complex associated like 2a</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_028013587.1"/>
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        <property type="gene ID" value="ENSXCOG00000003306.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="entry name" value="B12D"/>
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        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 4-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14256">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE MLRQ SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B12D"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="81" mass="9355" checksum="F151F8BBA4CA3552" modified="2018-12-05" version="1">MFRAVAEHAKRHPGLIPQFFFICLGMGGASLYLIRLARGPHVTWNKTDNPEPWNNLDPTYQYKFVAITTDYKNLKKEGPDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L385</accession>
    <name>A0A3B5L385_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Eukaryotic translation initiation factor 3 subunit I</fullName>
            <shortName evidence="8">eIF3i</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Eukaryotic translation initiation factor 3 subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">eIF-3-beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">eIF3 p36</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">EIF3I</name>
        <name evidence="8" type="synonym">EIF3S2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="function">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat STRAP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the eIF-3 subunit I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L385"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004316"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004363.1">
        <property type="protein sequence ID" value="ENSXCOP00000004316.1"/>
        <property type="gene ID" value="ENSXCOG00000003178.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161371"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001889">
        <property type="term" value="P:liver development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002040">
        <property type="term" value="P:sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000127">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03008">
        <property type="entry name" value="eIF3i"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR027525">
        <property type="entry name" value="eIF3i"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877:SF1">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24805">
        <property type="entry name" value="EIF3I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="7"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="49"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="319" mass="35608" checksum="1C08FB6D1CFEA9DA" modified="2018-12-05" version="1">SQKPILLQGHERSITQIKYNREGDLLFSVAKDTVANVWYSVNGERLGTYNGHTGAVWCVDCDDTKNVLTGSADNSCRLWDKQLALLNTNSAVRTCGFDFSGNIIMFSTDKQMGYQCFLNFFDLRDPQQMDNNPYLSVPCSECKITSAVWGPLGEFVIAGHENGEINQFSAKQSGEILKKAKEHTRQINDIQSSVDLTMVITASKDNTSKLFDSNSLDHIKTFRTDRPVNSAAISPIMDHVVMGGGQEAMDVTTTSTRIGKFEAFFHAAYEEEFGRVKGHFGPINCVAFHPDGKYSSGGEDGYVRIHYFDPHYFDFELEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L3F5</accession>
    <name>A0A3B5L3F5_9TELE</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L3F5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005403.1">
        <property type="protein sequence ID" value="ENSXCOP00000005342.1"/>
        <property type="gene ID" value="ENSXCOG00000004181.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="55" mass="6052" checksum="8B3A5AC9CE12283C" modified="2018-12-05" version="1">MTSLCVYSYAGTCTGTHPHTLTPTHAHTQMTPALPLFFLQRYLSSLIITISVISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L635</accession>
    <name>A0A3B5L635_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SRC kinase signaling inhibitor 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L635"/>
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    <dbReference type="Ensembl" id="ENSXCOT00000005347.1">
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        <property type="gene ID" value="ENSXCOG00000004140.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:actin cytoskeleton"/>
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    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="term" value="C:postsynaptic density"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="AIP3-like_C"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SRC KINASE SIGNALING INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03915">
        <property type="entry name" value="AIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Actin interacting protein 3-like C-terminal" evidence="2">
        <location>
            <begin position="104"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="212" mass="24166" checksum="4F53CD4E35C8BABC" modified="2018-12-05" version="1">KGHFSKILKSSFNLNSKTLFLCTQIFSFSPVFQSRTPRVTRLSPTQPALADQANRVSFASAENLETMSEPDIPIGFNRMNRLRQSLPLARSSSQAKLRAPGILFLQLGEEIRRVHLTHELTSLDTLRALIVHMFPQRLTMAMLRSPSTALLIKDETRNVFYELEDPRDVQDRCVIKIYCKEPIYGTYPGHHNPHIANGDLRVSVQSTELLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6X7</accession>
    <name>A0A3B5L6X7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Solute carrier family 25 member 15b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027888373.1">
        <property type="nucleotide sequence ID" value="XM_028032572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6X7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003534"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003573.1">
        <property type="protein sequence ID" value="ENSXCOP00000003534.1"/>
        <property type="gene ID" value="ENSXCOG00000002787.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114153805"/>
    <dbReference type="KEGG" id="xco:114153805"/>
    <dbReference type="CTD" id="565335"/>
    <dbReference type="GeneTree" id="ENSGT00730000110966"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000059">
        <property type="entry name" value="Mitochondrial ornithine transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000066">
        <property type="entry name" value="Mitochondrial ornithine transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF46">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 15B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="7"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="104"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="209"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="33069" checksum="43292A3B4BC94822" modified="2018-12-05" version="1">MAPHQVVQAIIDLSAGAVGGAACVFSGQPLDTAKVKMQTFPTLYRGFVHCIVSTYKQDGVRGLYQGTIPALMANIAENSVLFMSYGFCQQVIRFAAGLHHDAVLSNMQKACAGSVASIFSSLVLCPTELVKCRLQAMYEMEMSGRIAKSNNSVWSMVKSIMRNEGPQGFFQGLTTTIAREIPGYFCFFGAYELCRTGFADYMTCEKDDIGVAPIVFSGGIGGVCLWLVVYPMDCVKSRIQVMSMTRKQAGFFKTFMTIARSEGVRALYSGLTPTIVRCFPANGALFLGYEVSRKLMMKQFDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8F4</accession>
    <name>A0A3B5L8F4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Palm2 and akap2 fusion</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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            <taxon>Xiphophorus</taxon>
        </lineage>
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    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8F4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006818.1">
        <property type="protein sequence ID" value="ENSXCOP00000006735.1"/>
        <property type="gene ID" value="ENSXCOG00000005212.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:regulation of cell shape"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004965">
        <property type="entry name" value="Paralemmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10498:SF10">
        <property type="entry name" value="PALM2 AND AKAP2 FUSION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10498">
        <property type="entry name" value="PARALEMMIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03285">
        <property type="entry name" value="Paralemmin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="220"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="313"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="10"/>
            <end position="108"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="224"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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            <dbReference type="ARBA" id="ARBA00023054"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="371" mass="40614" checksum="55BC58C82B7AD9D7" modified="2018-12-05" version="1">QEKRKRQTEIDDKRSQLDELVLQLQHVKSKAMRERWLLQGMGAEEEAARRKQLEHDEEQGKKLEDIIQRLESEISALESEESQISAKEQVLRQRLKETERSIEDLQKINVQHDPKTGEQRILSANRVSPLEAASRGVKVYDDGRKVVYEVTSSGGVSTSTVENGWSSAQVDELVQRAARPSHAGGRGDSGRAYVSPADVDDLSPPSCITLQRETRLGMMPLSSAPITTQPGSSANRSSELSPMPQASTEHPVTMVFLGYQDVEDTSESRRLLGFDGAVKAEVVLIDEDDEKSLREKTVTDISIIDGTAADLVSGRPATSEAVSTELSSDGREPDSASSPPANPEANISPPPGLTPATGTDKRKRCQCCILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8Q3</accession>
    <name>A0A3B5L8Q3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">FA complementation group C</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="32473.ENSXCOP00000006830"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006914.1">
        <property type="protein sequence ID" value="ENSXCOP00000006830.1"/>
        <property type="gene ID" value="ENSXCOG00000005283.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016390"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="P:cellular response to oxidative stress"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:nucleotide-excision repair"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="FANCONI ANEMIA GROUP C PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16798">
        <property type="entry name" value="FANCONI ANEMIA GROUP C PROTEIN FANCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02106">
        <property type="entry name" value="Fanconi_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="416" mass="47442" checksum="099031A27EC89F41" modified="2018-12-05" version="1">KQGLVETNQMPFWLEKAIAWGQTDTPHTRNDISLHLKRLRDFLQHIQQFSSTTETLNRLPLLGQLLGRLCWNSHVTAEGESILWKIGKCCMCFCLLALYSEHPSNAVERKANQWIQKVLCHLATDEEDGAALGLMKHLGVPTKEYHLKVLKKVFCFYSFCLKAYLCSCGSIWAVSEACVPLITCPEASPLIGALLQQQMTCIRESLSENFLDALHSAYSRYRKCFYCIFSLCFFFFPEMPQESLKNHLIWVSSSLQRLTEEEEDEEDSDIFEAWFLLVQCGYWVQMVVQLLVTSGPEDCGPLLWLLTFYHHPTNRWHHRASQLVQAKAAWDHLYSLFSIVAHLLPADHLQSFISLLAPKPQQPSLAPSLTLNLLVNFATFGQLPQSISAEITQKVSKLSSLKPLHSASHLSCYHKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L927</accession>
    <name>A0A3B5L927_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">BCL2 interacting protein 3 like b</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NIP3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027881854.1">
        <property type="nucleotide sequence ID" value="XM_028026053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L927"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006965"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007053.1">
        <property type="protein sequence ID" value="ENSXCOP00000006965.1"/>
        <property type="gene ID" value="ENSXCOG00000005383.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114149929"/>
    <dbReference type="KEGG" id="xco:114149929"/>
    <dbReference type="CTD" id="554650"/>
    <dbReference type="GeneTree" id="ENSGT00390000013415"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097345">
        <property type="term" value="P:mitochondrial outer membrane permeabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010548">
        <property type="entry name" value="BNIP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15186:SF10">
        <property type="entry name" value="BCL2 INTERACTING PROTEIN 3 LIKE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15186">
        <property type="entry name" value="RE48077P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06553">
        <property type="entry name" value="BNIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="83"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="221" mass="24219" checksum="766EE04FAC4663BB" modified="2018-12-05" version="1">MSDAANVTSTDNNGDSGLNGSWVELEMNRTHQAQSSSPAGPAPGLLPLPQVEEEEAMVGGLEHVPSSSSIHNGDMEKILLDAQHESSRSNSSCDSPPRPHSPQDEGQIIFDVDTTGRRDSQSEEDALDKERDMDILMKDADWVADWSSRPENIPPKEFHFRHPRRSVMLSMRKTSAMKKGGIFSAEFLKVFIPSLLLSHILALGLGVYIGKRLITPPASSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9B1</accession>
    <name>A0A3B5L9B1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the POU transcription factor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027881806.1">
        <property type="nucleotide sequence ID" value="XM_028026005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9B1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006341"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006418.1">
        <property type="protein sequence ID" value="ENSXCOP00000006341.1"/>
        <property type="gene ID" value="ENSXCOG00000004885.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114149898"/>
    <dbReference type="KEGG" id="xco:114149898"/>
    <dbReference type="CTD" id="30333"/>
    <dbReference type="GeneTree" id="ENSGT00940000165709"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035284">
        <property type="term" value="P:brain segmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043697">
        <property type="term" value="P:cell dedifferentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009953">
        <property type="term" value="P:dorsal/ventral pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007398">
        <property type="term" value="P:ectoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009880">
        <property type="term" value="P:embryonic pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001706">
        <property type="term" value="P:endoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055113">
        <property type="term" value="P:epiboly involved in gastrulation with mouth forming second"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031101">
        <property type="term" value="P:fin regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030902">
        <property type="term" value="P:hindbrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007498">
        <property type="term" value="P:mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030917">
        <property type="term" value="P:midbrain-hindbrain boundary development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016331">
        <property type="term" value="P:morphogenesis of embryonic epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042663">
        <property type="term" value="P:regulation of endodermal cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000161">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000022">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF128">
        <property type="entry name" value="POU DOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="12">
        <location>
            <begin position="254"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="346"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="348"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="180"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="189"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="212"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="476" mass="52544" checksum="C63F089892CF5568" modified="2018-12-05" version="1">MSDRSQTPEIQSRPYDFSRAGACNTQVLGQEGTGNSSCFQLPHGVLPDPSLLYSKPAYGGITPAPTQTFLPFPHVANDYRGSDLQAGDFGQPKHWYPFPAPEYTGQVPGVIAATQPTNLSPPIAETREQIRLPEIKTEKDTGYDYPTEMKVQQYPIPAPSTSMTHGVFYPPTWNPSFWPGITHITQPGSNNQNPSASSTSSPSMSPSPPSNGTQGNAFFSVNANQVPSQPQAQNTAPNTRSSGSSSGGCSDSEEENLTTEELEQFAKELKHKRITLGFTQADVGLALGNLYGKMFSQTTICRFEALQLSFKNMCKLKPLLQRWLDEAETSENPQEMYKIERVFVDTRKRKRRTSLEGAVRSALEAYFIKCPKPNTQEITQISDDLGLERDVVRVWFCNRRQKGKRLALPLDEECEGQYYEQSPSPLNMAPTPIQGQAYPSSSYTGTPPPTLYMAPLHRPDVLKHALHPGLVGHLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LA68</accession>
    <name>A0A3B5LA68_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Vigilin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">High density lipoprotein-binding protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Appears to play a role in cell sterol metabolism. It may function to protect cells from over-accumulation of cholesterol.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LA68"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007737"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007834.1">
        <property type="protein sequence ID" value="ENSXCOP00000007737.1"/>
        <property type="gene ID" value="ENSXCOG00000005943.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00900000141059"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034364">
        <property type="term" value="C:high-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071391">
        <property type="term" value="P:cellular response to estrogen stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22405">
        <property type="entry name" value="KH-I_Vigilin_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22413">
        <property type="entry name" value="KH-I_Vigilin_rpt10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22414">
        <property type="entry name" value="KH-I_Vigilin_rpt11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22415">
        <property type="entry name" value="KH-I_Vigilin_rpt12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22416">
        <property type="entry name" value="KH-I_Vigilin_rpt13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22417">
        <property type="entry name" value="KH-I_Vigilin_rpt14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22418">
        <property type="entry name" value="KH-I_Vigilin_rpt15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22406">
        <property type="entry name" value="KH-I_Vigilin_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22407">
        <property type="entry name" value="KH-I_Vigilin_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22408">
        <property type="entry name" value="KH-I_Vigilin_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22409">
        <property type="entry name" value="KH-I_Vigilin_rpt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02394">
        <property type="entry name" value="KH-I_Vigilin_rpt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22410">
        <property type="entry name" value="KH-I_Vigilin_rpt7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KH-I_Vigilin_rpt8"/>
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        <property type="entry name" value="vigilin isoform X1"/>
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        <property type="entry name" value="vigilin isoform X1"/>
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        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="13"/>
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        <property type="entry name" value="SCP160"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="VIGILIN"/>
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    <keyword evidence="5" id="KW-0153">Cholesterol metabolism</keyword>
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            <end position="210"/>
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    <sequence length="1257" mass="140209" checksum="73BA3912CA83C6CF" modified="2018-12-05" version="1">MSSVAVLTQESFIEHRSGLLPEQSEEEEELPTYKDAFPPLPEKAATPEGTQETSNAWTSKIRPLKSSIITQVFHVPLEERKYKDINQFGEGEQAKVCVDIMHKTGAHLELSLAKDQGLSIMVSGKLDAVMKARKEIVSRLQTQASATVAIPKEHHRFVIGKNGEKLQELELKTATKIQIPRPDDSSNQIKISGTKEGLEKAKHEILLISAEQDKRAVERVNIDKVYHPFITGAYNKIVGEMMQETGARINVPPPSVNKNEIVITGEKEQVALAVALIKKIHEEKKKSTTTIAVEVKKSQHKYVVGPKGNTLQEILDRTGVSVEIPPSDNSSETVILRGEPDRLGQALTEVYAKANSYTVSSVSAPSWLHRFIIGKKGQNLAKITQQMPKVHIEFTEGEDKITLEGPTKDVQVVQSQIEAIVTDLVSRMDYAEITVDPKFHRHLIGKGGVNINRIKELHKVTVRIPPDNEKSNLIRIEGDPQGVQEAKKELLELASRMENERTKDLIIEHRLHRTIIGQKGEKIKEVRDKFPEVIINFPDPAQKSDIVQLRGPRNEVEKCSKFMQKMVAEMVENSQTLSVPIFKQFHRNIIGKGGANIKKIREETNTKIDLPAENSNSEMIVITGKKANCEAARNRILAIQKELANITEMDVSIPSKLHNSLIGSKGRLVRSIMEECGGVHIHFPSEGSGIDKVTIRGPVEEVEKAKQQLLALAEEKQIKSFTAELHAKPEYHKFLIGKGGGNIRKVRDSTGARIIFPTSEDKDQELITVVGTEEAVRTAQKELEELIKSLDNIVEDTMNVDTKHHRYFVARRGQVLRDLADEYGGVMVSFPRTASQSDKITLKGAKECVEAAKKRMQEIVEDLEAQVTVDCVISQKFHRSIMGPKGSRIQQITRDHNVQIKFPEREDPQAPVQENGEANGEVKEPVDPNAPKKCDVILISGRKERCEAALEALKALVPVTIEVEVPFELHRYIIGQKGSGIRKMMDEFEVNIQVPPPDQQSDKIAITGLASHLDRAKEGLFERVKELQAEQAERALKSFKLTITVDPKHHPKIIGRKGAIITTIRTEHDVNIQFPEKNDENQDQITITGYEQNAIAAREAIQAIVDELEEMITETITLDSRVHARIIGARGKGIRKIMDEFKIDLRFPQSGAADPNLVTVVGRPEHVDEAIDHLLNLEEEYLSDVVENEAKMAYMRPPGGSVAAADEQRGHSKGFVVREAPWATGNEKAPDMSSSEDFPSFGAPVQAKASPWGPKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBQ2</accession>
    <name>A0A3B5LBQ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBQ2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008272"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008370.1">
        <property type="protein sequence ID" value="ENSXCOP00000008272.1"/>
        <property type="gene ID" value="ENSXCOG00000006345.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166975"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14216">
        <property type="entry name" value="STKc_SRPK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001651">
        <property type="entry name" value="SRSF protein kinase 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000105">
        <property type="entry name" value="SRSF protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000163">
        <property type="entry name" value="SRSF protein kinase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF4">
        <property type="entry name" value="SRSF PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="45"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="203"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="256"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="368"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="256"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="287"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="315"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="383"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="393"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="587" mass="66612" checksum="A0BFBFC0F8768408" modified="2018-12-05" version="1">ETSPQEPEEPEEILGSDDEEQEDPNDYCKGGYHHVKVGDLYNGKYHVIRKLGWGHFSTVWLAWDIQVKRFVAMKVVKSAEHYTETAVDEIKLLRSVRNSDPDDPKREMVVQLLDDFKISGVNGTHVCMVFEVLGHHLLKWIIKSNYQGLPLPCVKSIIKQVLQGLDYLHTKCQIIHTDIKPENILMSVDEPYVRKLAAEATEWQRAGAPPPSGSAISTAPAPKQTVKMSKNKKKKLKKKQKRQAELLEKCIMDMEEMEKTTETRKDDEEDKEPQSPKGRACAPLRHVSVEEFRKTPDNMRVGPDEPPEANCNGHAEVEERKSSCSNEDQHNGNAESPEELHKESPVHTLCNGVESTDFNELYCEMDGREAHSSGVSQRRLRSGRGELERSILQDDDEQDETEDKLTAGSLLVNPLEPGNAEKIKVKIADLGNACWVHKHFTEDIQTRQYRSLEVLIGAGYSTPADIWSTACMAFELATGDYLFEPHSGEDYSRDEDHIALIIELLGSVPRKLIMSGKYSKDFFTKKGELKHITKLKPWGLLEVLVEKYEWPREEAECFSDFLLPMLELVPEKRATAAECLCHAWLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBW7</accession>
    <name>A0A3B5LBW7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Ribosomal RNA-processing protein 8</fullName>
            <ecNumber evidence="15">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Probable methyltransferase required to silence rDNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
            <location evidence="1 15">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the methyltransferase superfamily. RRP8 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBW7"/>
    <dbReference type="SMR" id="A0A3B5LBW7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008015"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008113.1">
        <property type="protein sequence ID" value="ENSXCOP00000008015.1"/>
        <property type="gene ID" value="ENSXCOG00000006156.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006189"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005677">
        <property type="term" value="C:chromatin silencing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046015">
        <property type="term" value="P:regulation of transcription by glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2150:FF:000001">
        <property type="entry name" value="Ribosomal RNA-processing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000068">
        <property type="entry name" value="Ribosomal RNA-processing protein 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2150">
        <property type="entry name" value="Ribosomal RNA-processing protein 8, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007823">
        <property type="entry name" value="RRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042036">
        <property type="entry name" value="RRP8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787:SF0">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05148">
        <property type="entry name" value="Methyltransf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 15" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 15" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="14"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="380" mass="42676" checksum="F61046C595388B51" modified="2018-12-05" version="1">MKNKKRSKNKFRQSKPEDDADGARTADKREAAEDVKKDPHNGNGENISQMANRKQKKEKVKRKNTEREADGCSAARGENQPEAERLKSGSEGSAEGLKHGAGVQVASTNRQKPELSKERSLKRQKLQKILLRQERVGAEGPVERMQMEVAVPEAAAEQSRSDSLRSRMEQRLEAARFRYINERLYSTASGEARRMFQQDPEAFWVYHRGYTAQVQRWPANPLDAIIAYIHKRSPSLVVADFGCGDCKIARSVKNKVHSFDLAAACDLVTVCDMAKVPLGDDSVDIAVFCLSLMGTNLVEFLAEANRVLKMRGVLKVAEVASRFDNMQNFISALAGLGFKMTSKDTENSHFFSFEFVKTGNVPQNVKKFGLQLRPCLYKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCG4</accession>
    <name>A0A3B5LCG4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCG4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008230"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008328.1">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="250"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="150"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="201"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <source>
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        </source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="560" mass="65235" checksum="EA90AE8967AB7C03" modified="2018-12-05" version="1">HTDSSSCSSFKTIFMCLLFQSDLTLLKLKAIEKVKNSDLKRTEGRIDDLVRSNCDLRRQVDEQQKILERYKERLNKCVKISQQLLIEQSKREKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQKLIKQQEQINSQREDIERQRKLLGKRKPPVVQMPPPNMEQNKRKSRNNGQENETLSLAAYREQEEIFKLRIGRLKKEEAEIQTELEQLERVRNLHIRELKRIHNEDNSQFRDHPTLNDRYLLLRLLGRGGFSEVFKAFDLTEQRYVAIKIHQLNKNWREEKKQNYHRHACREYRIHKELDHPRIVKLYDYFSIDRDSFCTVLEYCGGNDLDFYLKQNKSMTEKEGRSIIMQLVNALKYLNQIRPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIVDDDSSADGMDLTSQGAGTYWYLPPECFVMGEDPPKISNKVDVWSVGVIFYQILYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVTTEAKAFIRRCLAYHKEDRVDVLQLASDPFLMPNIRKALGSNTMSLLPSPSTSSCYSSSASNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LD71</accession>
    <name>A0A3B5LD71_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase</fullName>
            <ecNumber evidence="6">2.7.8.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">GlcNAc-1-P transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">N-acetylglucosamine-1-phosphate transferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes the initial step of dolichol-linked oligosaccharide biosynthesis, transfering GlcNAc-1-P from cytosolic UDP-GlcNAc onto the carrier lipid dolichyl phosphate (P-dolichol), yielding GlcNAc-P-P-dolichol embedded in the cytoplasmic leaflet of the endoplasmic reticulum membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a di-trans,poly-cis-dolichyl phosphate + UDP-N-acetyl-alpha-D-glucosamine = an N-acetyl-alpha-D-glucosaminyl-diphospho-di-trans,poly-cis-dolichol + UMP</text>
            <dbReference type="Rhea" id="RHEA:13289"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19507"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58427"/>
            <dbReference type="EC" id="2.7.8.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:13290"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 4 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.15" evidence="6"/>
    <dbReference type="RefSeq" id="XP_027887330.1">
        <property type="nucleotide sequence ID" value="XM_028031529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LD71"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008792"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008898.1">
        <property type="protein sequence ID" value="ENSXCOP00000008792.1"/>
        <property type="gene ID" value="ENSXCOG00000006706.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114153165"/>
    <dbReference type="KEGG" id="xco:114153165"/>
    <dbReference type="CTD" id="1798"/>
    <dbReference type="GeneTree" id="ENSGT00390000011424"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003975">
        <property type="term" value="F:UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06855">
        <property type="entry name" value="GT_GPT_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048439">
        <property type="entry name" value="DPAGT1_ins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033895">
        <property type="entry name" value="GPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10571">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--DOLICHYL-PHOSPHATE N-ACETYLGLUCOSAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10571:SF0">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--DOLICHYL-PHOSPHATE N-ACETYLGLUCOSAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21383">
        <property type="entry name" value="DPAGT1_ins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="193"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="377"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="DPAGT1 insertion" evidence="22">
        <location>
            <begin position="316"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00017659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00033238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00045078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF21383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="406" mass="45767" checksum="D89891038453A4A0" modified="2018-12-05" version="1">MSPVPVLPLVISCFMSALGCMATLKLIPAFKDHFISARLYGMDLNKPNKDRVPESQGVISGTVFLIILFCFIPVPFLSCFVGDQCTGFPHDEFVQLIGALLAICCMIFLGFADDVLNLRWRHKLLLPTMASLPLLMVYFTNFGNTVIVVPKPFRALLGMHLDLGILYYVYMGMLAVFCTNAINILAGINGIESGQALFISGSIIVFNLLELSGDYRDDHVFSLYFMIPFFFTTLALFYHNWYPSSVFVGDTFCYFAGMTFAVVGILGHFSKTMLLFFIPQVVNFIYSLPQLFHIIPCPRHRLPRLDPDTGKLGMSYSKFKKKDLSQLGLLILQVAELLKVLEVRRGQDGDGDFIECNNMTLINLILKFFGQIHERNLTVIMLIIQVMGSALAFGIRYHLVRLFYDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDI8</accession>
    <name>A0A3B5LDI8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDI8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005929"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005995.1">
        <property type="protein sequence ID" value="ENSXCOP00000005929.1"/>
        <property type="gene ID" value="ENSXCOG00000004593.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182881"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF71">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="92"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="817" mass="93333" checksum="F8A57869BCDDB5FB" modified="2018-12-05" version="1">MVFRSEEMCLAQLFLQSGSEYDCISELGELGLVEFRDLNPSVSSFQRRFVSEIKRCEEMERILGFLLREIQKAKIAVPQEDESPLAPPPRQVLEIMEQLQRLEMELSEVAKNKDKLQRNLLELTEYTHMLKITRSFIHSRSRHEALGPQYEEFPTMETDSATGSTGMQRLGAKLGFISGLIQRVKVEAFERMLWRVCKGYTILSYTEVDESLADLDTGEINKSVVFLISFWGDQIGHKVKKICDCYHCHLYPHPENDEERADVLDSLRTRIQDLNNVLHRTDDYLRQVLQKAAESAYAWVVQVKKMKAIYHILNLCSFDVTNKCLIAEVWCPVSDLANLRGALEKGSRKGDATVPSFVNRIPSSDTPPTLLRSNKFTTGFQSIVEAYGVGDYREVSPAPYTIITFPFLFAVMFGDLGHGMIMSLFALWMVLTEKNQKKKRSSNEIWMTFFHGRYIILMMGLFSIYTGLIYNDCFSKSLNIFGSGWSVKAMFTNQQWTNKTVQTNALLTLDPNVSRVFSGPYPFGIDPIWNLAVNRLSFLNSYKMKMSVIVGVIHMSFGVVLSVFNHLHFGQKFSVYLLFLPELLFLICLFGYLVFMILYKWLAFSAHNSSQAPSILIHFINMFVMQGKDVTPLYPGQTGLQVFLLLIAVMCVPVLLLGKPLYLYWQQRGGKGLRRRRVINISCTVLQFDFADVLMHQAIHTIEYCLGCVSNTASYLRLWALSLAHAQLSEVLWAMVMRLGFKITTKVGVVFLVPVFGLFAMLTVSILLVMEGLSAFLHALRLHWVEFQNKFYHGTGVKFLPFDFSQLPSVFEQDGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LE81</accession>
    <name>A0A3B5LE81_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LE81"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007991"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008089.1">
        <property type="protein sequence ID" value="ENSXCOP00000007991.1"/>
        <property type="gene ID" value="ENSXCOG00000006136.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177818"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="2">
        <location>
            <begin position="179"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="332" mass="37370" checksum="EE8B226CA6FF6B69" modified="2018-12-05" version="1">MTPSCSTLTAHYSLTTNFRNAYMGIMYDVMLRRLKKAAYKYSRETRQKLDEVSADEEEPQATSSEPGPQIPRGPQGVSAQPPATTTGQDAYAELKNLRLVIRSKNADENNEEYLKDVWPTVLSTTNHEGAKKKGTVESTKATNTRWGRWEDILLDPELEIGPAQPTSKKRPQETPEKPEHPEWTIYTDGSRKGSDQSAYWGFILKQDGKERCRQKGKAPGSAQAGEVTAVLEGLLELVKRKIKSARLVTDSYYCAQALREDLAIWEENGFETAKGKPVAHKDLWKKIAELKLQLEIDVEHQKAHTHEGAHWRGNDEVDCYSWLSAAPASKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEN8</accession>
    <name>A0A3B5LEN8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LEN8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009035"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009144.1">
        <property type="protein sequence ID" value="ENSXCOP00000009035.1"/>
        <property type="gene ID" value="ENSXCOG00000006889.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182850"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030032">
        <property type="term" value="P:lamellipodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032402">
        <property type="entry name" value="AbLIM_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051618">
        <property type="entry name" value="Actin-binding_LIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24213">
        <property type="entry name" value="ACTIN-BINDING LIM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24213:SF0">
        <property type="entry name" value="ACTIN-BINDING LIM PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16182">
        <property type="entry name" value="AbLIM_anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02209">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00153">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HP" evidence="5">
        <location>
            <begin position="61"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="127" mass="14992" checksum="3BA627E5F013DA8D" modified="2018-12-05" version="1">MHSLQVGIGRMILKEEMKARSGVQDNNHWRSARSSQSNSKETLNNLEYNSLNGCKSLCLIALHYKYLCFFCYILRGQQRLPSDVDRARLERHLSPEEFYRVFGMSMSAFDRLAQWKKNELKKQVRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFG9</accession>
    <name>A0A3B5LFG9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM CPEB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027886605.1">
        <property type="nucleotide sequence ID" value="XM_028030804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFG9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008426"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008532.1">
        <property type="protein sequence ID" value="ENSXCOP00000008426.1"/>
        <property type="gene ID" value="ENSXCOG00000006460.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114152790"/>
    <dbReference type="GeneTree" id="ENSGT00940000154998"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000900">
        <property type="term" value="F:mRNA regulatory element binding translation repressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008135">
        <property type="term" value="F:translation factor activity, RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000766">
        <property type="term" value="P:negative regulation of cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19757">
        <property type="entry name" value="Bbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12724">
        <property type="entry name" value="RRM1_CPEB2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12726">
        <property type="entry name" value="RRM2_CPEB2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000008">
        <property type="entry name" value="Cytoplasmic polyadenylation element-binding 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.640.40:FF:000001">
        <property type="entry name" value="Cytoplasmic polyadenylation element-binding 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000009">
        <property type="entry name" value="cytoplasmic polyadenylation element-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.40">
        <property type="entry name" value="Cytoplasmic polyadenylation element-binding protein, ZZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032296">
        <property type="entry name" value="CEBP_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038446">
        <property type="entry name" value="CEBP_ZZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034819">
        <property type="entry name" value="CPEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12566">
        <property type="entry name" value="CYTOPLASMIC POLYADENYLATION ELEMENT BINDING PROTEIN CPEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12566:SF2">
        <property type="entry name" value="CYTOPLASMIC POLYADENYLATION ELEMENT-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16366">
        <property type="entry name" value="CEBP_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16367">
        <property type="entry name" value="RRM_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="487"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="595"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="81"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="225"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="251"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="319"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="348"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="744" mass="81382" checksum="F0175FB14C480B7A" modified="2018-12-05" version="1">MGDYGFGVLVQNNTGNKSAFPVRIHPHLQPQHHHQNVSQSPAAFINSTTATGNGTTGGSPWLFPTSATHNSVQDEILGGAEKPKAQQQQEMQETQEKQQQLSPGGHQETGSGGGIIAELEKSRSEEAKTENGTSEGNNGKEKQLRLESPVLTGFDYQETSGLGGTGPVQSSTTSSSLTGFNNWSAAIPPAPSTIINEDVSFFNPASANNGPLLFQNFSHHVSPGFGGNFSPQIGPAPALSQHHPAPPPHPHFQHPHNQHQQHRRSPASPHPPPPFSHRNPAFNQLPHLSNSLNKPPSPWGPASYQSPSPSPGSSTSWSPGGGYGSGGGGWGGSQGRDYRRGLNGGMTPINSISPLKKTFPNNHVASQKYPRNSPAGFNPKSWMDDGVGRDNIFPFQERTRSFDSFNMNTLENSLIDIMRAEQDTLKGRLGFPHPGADNPLPLNGHSSLFPMEDSFPDDERADQGLAGLGSPHCFPHQNGERVERYSRKVFVGGLPPDIDEDEITASFRRFGHLFVDWPHKAESKSYFPPKGYAFLLFQDESSVQALIDACIEEDGKLYLCVSSPTIKDKPVQIRPWNLNDSDFVMDGSQPLDPRKTIFVGGVPRPLRAVELAMIMDRLYGGVCYAGIDTDPELKYPKGAGRVAFSNQQSYIAAISARFVQLQHGEIDKRVEVKPYVLDDQLCDECQGTRCGGKFAPFFCANVTCLQYYCEYCWAAIHSRAGREFHKPLVKEGGDRPRHISFRWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFI2</accession>
    <name>A0A3B5LFI2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LCCL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFI2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009335"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009446.1">
        <property type="protein sequence ID" value="ENSXCOP00000009335.1"/>
        <property type="gene ID" value="ENSXCOG00000007110.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159330"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.20">
        <property type="entry name" value="LCCL-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004043">
        <property type="entry name" value="LCCL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036609">
        <property type="entry name" value="LCCL_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03815">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00603">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69848">
        <property type="entry name" value="LCCL domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50820">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LCCL" evidence="1">
        <location>
            <begin position="51"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="94" mass="10408" checksum="87B6D2D40AECBBDE" modified="2018-12-05" version="1">MTYNKTNAKQVILSTTRLSGWFQAAEGLCLVLFPCDVRAGKINLPEFIAKCPAHCKETKQQVYGTGVFASISSICNAAIHRCLDWKMNIAERVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFJ0</accession>
    <name>A0A3B5LFJ0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFJ0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009726.1">
        <property type="protein sequence ID" value="ENSXCOP00000009612.1"/>
        <property type="gene ID" value="ENSXCOG00000007324.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000271297"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="132" mass="12620" checksum="72CC37978829EC57" modified="2018-12-05" version="1">FCCSYRPNPGSGDGPSPLPGHGPSPGSKPGPRHGPSPGSRHGPSPGSRHGPSSSSRHGPRTGSGDVPSPGLGHGPSSGSRHGHSPGLGHGPSPGSTHGPSSGSRHGPRTGSKYGASPGSNPGPGHSSSPGPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGT9</accession>
    <name>A0A3B5LGT9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clathrin/coatomer adaptor adaptin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGT9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010072"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010191.1">
        <property type="protein sequence ID" value="ENSXCOP00000010072.1"/>
        <property type="gene ID" value="ENSXCOG00000007646.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182838"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050840">
        <property type="entry name" value="Adaptor_Complx_Large_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22780">
        <property type="entry name" value="ADAPTIN, ALPHA/GAMMA/EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="6">
        <location>
            <begin position="7"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="247" mass="27537" checksum="868807D1D9AA372D" modified="2018-12-05" version="1">MCLSVQGYLFISVLVNSNSELIRLINNAIKNDLSSRNPTFMCLALHCIANVGSREMAEAFASEIPRILVAGDTMDSVKQSAALCLLRLYKTSPDLVLMGEWTSRVVHLLNDQHMGVVTAAISLITCLSQKNPDEFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSCKLLRLLQCYPPPEDGAVKGRLVECLETILNKAQEPPKSKKVQHSNAKNAILFEAISLIIHYDSLNDLIFLREMHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LH83</accession>
    <name>A0A3B5LH83_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phospholysine phosphohistidine inorganic pyrophosphate phosphatase</fullName>
            <ecNumber evidence="5">3.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LHPP</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Phosphatase that hydrolyzes imidodiphosphate, 3-phosphohistidine and 6-phospholysine. Has broad substrate specificity and can also hydrolyze inorganic diphosphate, but with lower efficiency.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>diphosphate + H2O = 2 phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_027885268.1">
        <property type="nucleotide sequence ID" value="XM_028029467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LH83"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007229"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007321.1">
        <property type="protein sequence ID" value="ENSXCOP00000007229.1"/>
        <property type="gene ID" value="ENSXCOG00000005589.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114151924"/>
    <dbReference type="GeneTree" id="ENSGT00940000159002"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07509">
        <property type="entry name" value="HAD_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000051">
        <property type="entry name" value="Phospholysine phosphohistidine inorganic pyrophosphate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006355">
        <property type="entry name" value="LHPP/HDHD2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01458">
        <property type="entry name" value="HAD-SF-IIA-hyp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF44">
        <property type="entry name" value="PHOSPHOLYSINE PHOSPHOHISTIDINE INORGANIC PYROPHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="274" mass="29645" checksum="332A0059F991C710" modified="2018-12-05" version="1">MADTGWPGCTKSLKGVILDLCGVLYDSGEGDGVAIPGSVEAVKRLKESDLQLRFCTNETQATREKFVAKLQRLGFDISVSEVFPPAPAAIAVLKERGLRPHLLVHDGLLPEFDAVDKTNPNCVIIGDAAENFSYQNLNEAFRVLIGLEKPVLFSLGQGRYYKETDGLKLDVGVYMKALEYACDLKAEVIGKPSPTFFQSVLSDMGLKPHETLMIGDDLVNDVGGAQYCGMKGVQVRTGKYRPSDERHPTVIADATVDNLAHAVDAILGQRGCSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIQ8</accession>
    <name>A0A3B5LIQ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIQ8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010649.1">
        <property type="protein sequence ID" value="ENSXCOP00000010525.1"/>
        <property type="gene ID" value="ENSXCOG00000007965.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157829"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000497">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752:SF13">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="128"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="170"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="28"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="454" mass="50155" checksum="A6FF8F9598BEC8AC" modified="2018-12-05" version="1">SGFFSALSETRPVPLCVCGNDHTISRESNVLVIIIVAATKTLHAVTSILILNLAVSDLLVGIGVMPFVAMSIMNTGWVRCPVLCLYVGYSSTVYCTASVLTLTAIALDRYYSIIDCLRYSSRCTLWRTSAVVLWIWLLALVICSPPLLGWSSVSYVVPMYNCAITWASTVSYTAFMAVLIYLMPAVVILFCYVNIIRVARSHARRIHSLQDSVQRSRSPSCPGDSSHQNCGMRGLFSILPHHGGLRLVLIIFAFLFCWTPYICVALVQATETAITGQSTLVPDSAITFSYWLMLLNSDVNPLLYALLSQRFQIALQGLWHRIRAHLCSLFSTEWEVRGDGDDVRITDPGTLTTAHPSTHASTESLNCDNSKLSASVFTVSTDFKSHSEGYLCNVCHHENTPSVFQDAASEKTHNLQVPSRPQEGSRLPFSALTKERQATFFYGQITVSVEHDVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJ30</accession>
    <name>A0A3B5LJ30_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Heparan sulfate 2-O-sulfotransferase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">2-O-sulfotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">HS 2-O-sulfotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Heparan sulfate 2-sulfotransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfotransferase 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJ30"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009621"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009735.1">
        <property type="protein sequence ID" value="ENSXCOP00000009621.1"/>
        <property type="gene ID" value="ENSXCOG00000007331.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063408"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004394">
        <property type="term" value="F:heparan sulfate 2-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055113">
        <property type="term" value="P:epiboly involved in gastrulation with mouth forming second"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060828">
        <property type="term" value="P:regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000047">
        <property type="term" value="P:regulation of cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000534">
        <property type="entry name" value="Heparan sulfate 2-O-sulfotransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007734">
        <property type="entry name" value="Heparan_SO4_2-O-STrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129">
        <property type="entry name" value="HEPARAN SULFATE 2-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12129:SF14">
        <property type="entry name" value="HEPARAN SULFATE 2-O-SULFOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00093675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="337" mass="39599" checksum="F3AA37FD796FCA6C" modified="2018-12-05" version="1">TPRHRSLAPPQMIAVKFFTRAIARHEVREIEQRHTQDGLREREAAAAALTNSEDDLVIIYNRVPKTASTSFTNIAYDLCGKNRYHVLHINTTKNNPVMSIQDQVRFVKNVTEWREMKPAFYHGHVSFLDFTKFGVKRKPIYINLIRDPIERLVSYYYFLRFGDDYRPGLRRRKQGDKKTFDECVSAGGSDCAPEKLWLQIPFFCGHYSECWNVGSQWALEQAKYNLVNEYMLVGVTEELEDFVMMLEAALPRFFRGATELYKTGKKSHLRRTSEKKPPTKESIAKLQQSAIWKMENDFYEFALEQFQFVRAHAVREKDGELYLLAQNFFYEKIYPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LK36</accession>
    <name>A0A3B5LK36_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Solute carrier family 16 member 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027872541.1">
        <property type="nucleotide sequence ID" value="XM_028016740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LK36"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008249"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008347.1">
        <property type="protein sequence ID" value="ENSXCOP00000008249.1"/>
        <property type="gene ID" value="ENSXCOG00000006330.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114144190"/>
    <dbReference type="KEGG" id="xco:114144190"/>
    <dbReference type="CTD" id="9122"/>
    <dbReference type="GeneTree" id="ENSGT00940000158411"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000277">
        <property type="entry name" value="Solute carrier family 16 member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000141">
        <property type="entry name" value="Solute carrier family 16, member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF14">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="456"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="486"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="4">
        <location>
            <begin position="30"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="239"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="240"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="265"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="571" mass="61912" checksum="DC78FD2E36C32C42" modified="2018-12-05" version="1">MNLFGRDAMTTKKEKEKEIQYADPPDGGWGWMVVLHCFLVNVLVMGTLKSFGIFFVAFQDEFGGSAESISWIGSIMSSLRLSGAPIASVACAKLGPRVTSIAGAVLVSGGFIISIFASSVIYLYISMGVIVGMGFALLYQSFSVITALYFRKRLATAYAIGRSGMGLTFALAPFTQLLLDQYAWQGAMLILGGLMLNLVASGMLLRPINVKPPVTNSTSLPIQKSPSISLKSSAEKEYPKSCLSSSSPLSNGVSKSDSLQSSPTPHRDNENLKEQSIHGQESQLLNPELTRLVLNNINGHEPQHVLGQCKPASPDSPADMNGTMNGSTTVGVPLHTGVPTAVAVVKPKLLDFTLLKDPFFCIYTWSLVFSQLAYFIPYFHLSARARNLGIDAMDASFIISVAGITETIAQLASGWVTDRNLFHKYHFHKAYLILCGLVNMLSPLATSYILLMVYAVFFAIFCGGYMALLLPVLVDLVGSEKLNNSMGFSMFFVGLGCLTGPPLAGFLYDYTQTYDCSFYLAGLCYLLSSLSLFMEPLAQRWKAKKKLSQSQSTESSCKINGCFTPDQNGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKI4</accession>
    <name>A0A3B5LKI4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/IRO homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKI4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008404"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008510.1">
        <property type="protein sequence ID" value="ENSXCOP00000008404.1"/>
        <property type="gene ID" value="ENSXCOG00000006446.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159483"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000003">
        <property type="entry name" value="Iroquois-class homeobox protein IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003893">
        <property type="entry name" value="Iroquois_homeo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11211:SF17">
        <property type="entry name" value="IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00548">
        <property type="entry name" value="IRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="81"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="83"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="141"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="169"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="199"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="312" mass="34774" checksum="A1D13D9E127B29BB" modified="2018-12-05" version="1">MRRFPPEGKAPRVSPPRRQSFFRTSSGILDLSSAADHPQIIGTFLMLQNPGYDPSSGVSGSLDYHAFGPLGAYPYGDPTYRKNATRDATATLKAWLNEHRKNPYPTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKMTWTPRNRSEDEEEEDAIDLERTEEDDEPLKLTEEETELRSDSGDGPLEALRASETKRTSSPSSLDSCGATQGPNPAPKPKLWSLAEIATSSRGRSSLSHGPALPRHLYYASPFIPGYSSFRPLGPLQGGSHINGLQQKMLQRAEAAARDCRLRSQSQTELQELKRANINNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LL41</accession>
    <name>A0A3B5LL41_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin/ISG15 ligase TRIM25-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LL41"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012962.1">
        <property type="protein sequence ID" value="ENSXCOP00000012808.1"/>
        <property type="gene ID" value="ENSXCOG00000009676.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286922"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="15"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="6">
        <location>
            <begin position="147"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="23762" checksum="5ED470DA81DBE28A" modified="2018-12-05" version="1">MAQKGVQLDRKNFSCSVCLDILKDPVMIPCGHSYCLKCIKNVWDEKNKKGVYSCPQCRQTFTPRPVLVKNIMLAGLTEQLTGLGASLTEQSYAGPEDVACDICTGKKLKAIKSCLMCLVSYCEKHLQPHYDVAQLKKHQLVQPSKQLHDNICSRHDEVMKMFCRTDQQTICYVCMVDEHKGHDVVSAAAERKEKQKDLEVKPQTIDPQCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPJ1</accession>
    <name>A0A3B5LPJ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPJ1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009873.1">
        <property type="protein sequence ID" value="ENSXCOP00000009759.1"/>
        <property type="gene ID" value="ENSXCOG00000007414.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182774"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001480">
        <property type="entry name" value="Si:cabz01071911.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000260">
        <property type="entry name" value="Spalt-like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000711">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001134">
        <property type="entry name" value="Zinc finger protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF151">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="277"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="305"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="333"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="361"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="389"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="442"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="496" mass="57752" checksum="88BB329179D3B881" modified="2018-12-05" version="1">KKTKTCSSKRKLRKKSRAHQIRYFCKICHKTFSLSTSLTRHMKIHNGNKPFTCTVCRKSYRHKSGLIHHLRIVTVEKPFTCSLCEKHYHLKGALKNHMRIHTGEKLFTCSLCEKHYRFKSGLKNHMRFHTGEKPFSCVTCGKGFIRKNTLTVHMRVHIMKQKERCQKTKRQKSKTCSSKQNTHKKPHTDPKLYSCKICDKTFSQKNSATQHMKIHTGEKPFSCPTCGKSYSYKSGLIYHLRIHTGEKPFTCSLCGKHYPLKETLKNHMRIHTDEKPFSCPSCRKGFSQKSTLTRHMRIHTGEKRYSCVTCGKHFGYKCDLNIHMRIHTGEKPFTCPTCRKKYRQRATLSRHMTIHTGENPYTCVSCGKHFSCKSYLDIHMRIHTGEKPFSCVICGKRFCQKSNLSAHFQTHTGEKSFSCDLRFSRNIHLTAHMRTHTGEKPFPCGNCGKAFTCRSGLRQHMMTHTSEKLFSCGIYSNIDKYVSNQFVTYQRKHCIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPS2</accession>
    <name>A0A3B5LPS2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">O-acyl-ADP-ribose deacylase 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPS2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014284.1">
        <property type="protein sequence ID" value="ENSXCOP00000014108.1"/>
        <property type="gene ID" value="ENSXCOG00000010689.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175052"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="P:peptidyl-glutamate ADP-deribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADP-ribose_metab_enzymes"/>
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        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12521:SF0">
        <property type="entry name" value="ADP-RIBOSE GLYCOHYDROLASE OARD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12521">
        <property type="entry name" value="PROTEIN C6ORF130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="91" mass="10315" checksum="412F62757413A891" modified="2018-12-05" version="1">PASKMSSGPQPYVTGDLFSCPRDESLAHCISEDCRMGAGIAVMFKKKFGRLLKSLFWKKCFMFLNFNRALYNLLPVNTEGIFYRSSTFTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPY2</accession>
    <name>A0A3B5LPY2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carotenoid/retinoid oxidoreductase family. CrtISO subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPY2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014168"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014345.1">
        <property type="protein sequence ID" value="ENSXCOP00000014168.1"/>
        <property type="gene ID" value="ENSXCOG00000010718.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="Retinol_saturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46091:SF2">
        <property type="entry name" value="AMINE OXIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46091">
        <property type="entry name" value="BLR7054 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="607" mass="67658" checksum="F19BDE3DE8B6AB00" modified="2018-12-05" version="1">MWLWVFAVLSCLLVALILFLITGKRYKVFSEKCLRPPGPLVTNSNERDARLKKGFRADRVPPALDAVVIGSGIGGLAAAAILSKAGKKVVVLEQHDLAGGCTHTFQNKGFEFDVGIHYLGQLHENSLLRVALDQITEGQLQFIKLEQHYDTVLLGPPGHCREYRIHAGKTEMADSLKKQFPGEEEAIDEFMKLIKVCVWEWACVTPLIAILKILPYPVVNLLIWTGLLDRLSPVFRLATASHSEMMSRLTQNKDLQALSAYFFYGVPPKESSFLINALLLHHYKRGAYYPYGGASEIAFHIVPVIQQAGSEVLVRAPVQRILINSQGKACGVTVLKGQQEIEVLAPVVISDAGIFNTFQKFLPQHIQKKPGKMFLLSMLRHGMGSFLVFVGLDGTKEELNIVSKNFWMYKGNDLDSLMEAYSSLNREQVLGNIPMMFITFPSAKDPTAAIRHPGKSCMTLLTMARYEWFEDWKETKVGKRGPEYLDLKNSMAQELLEWALTIFPQLRDKVSHSATPLTNVHYLGAPRGEMYGAEHNLERFGPETSAKTRPQTPVSGLYLTGQDVFCNGLAGALHGGLLCASAVLNQILYIDLVGLKTHLKHRNTKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQ55</accession>
    <name>A0A3B5LQ55_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQ55"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013070.1">
        <property type="protein sequence ID" value="ENSXCOP00000012910.1"/>
        <property type="gene ID" value="ENSXCOG00000009769.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234575"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03590">
        <property type="entry name" value="CLECT_DC-SIGN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033989">
        <property type="entry name" value="CD209-like_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="134"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="274" mass="31785" checksum="E513FC4C1EFA950D" modified="2018-12-05" version="1">MTEESLTRTMEKTQSQGCDSDGFNRLICEEEFHVEDHNSFHMSNPSSLGKDLVELHKQYKTAVENIYAAQKQLDDEMKSQKETKWELEHQTKRTEDYKVFDGVACISFYKFSVIALLSLTVDGCTHCPPGWLFINLVCYYFSSNNAGLKSWQKAREFCQIYGADLPVIDTKDKEKIVTYLLNHGQDPSETKSAFWFGLRDSNEEGTWQWLDGTSLVEGYWNVGQPDDYHTEDCAAVYPNENFFRAWNDLECGSRQNWICEKAPTYEFLNRTNNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQB5</accession>
    <name>A0A3B5LQB5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQB5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013232"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013395.1">
        <property type="protein sequence ID" value="ENSXCOP00000013232.1"/>
        <property type="gene ID" value="ENSXCOG00000010023.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154817"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045180">
        <property type="term" value="C:basal cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043515">
        <property type="term" value="F:kinetochore binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040001">
        <property type="term" value="P:establishment of mitotic spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000005">
        <property type="entry name" value="CLIP-associating protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF28">
        <property type="entry name" value="CLIP-ASSOCIATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOG" evidence="2">
        <location>
            <begin position="77"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="299"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="299"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="423" mass="46199" checksum="3F9053E5A613F68A" modified="2018-12-05" version="1">KKLEQTFIIFSRLGYKTFEDDESVDGRRSSSRAASVSGRKAVSMGSFRRPSAPRVHVAGAVDEEDFIRAFEDVPTVQIYSNREVEEAMTKIRDVLSDDKKDWELRVAALKKLRSLLLAGAPEFDCFLQQLRLLEASFKLSAKDLRSQVVREACITLGHLSAVLGSRFDHAAEAIMPPLLNLVPNSAKVMATSGVAAIRLILRHTHCPRLVPIISSSCSSKSVAVRRSDRIYLHGALLMETIKKGIHDADAEARSVARKCYWSYQGHFNREAELLFQGLESAYQRALQAHLRSGDALVSLPASDRSSSSSQESLNAPRRPGAASSPGALQRSRSNVDVNASASARSRMPAVSAAAAALSPGSYASLGQNQNVPLAPPTSQGSRVVLHHLVFILRASTDQEDERRNRCHGDGRPEPREGRLSVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQH3</accession>
    <name>A0A3B5LQH3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Inosine triphosphate pyrophosphatase</fullName>
            <shortName evidence="13">ITPase</shortName>
            <shortName evidence="13">Inosine triphosphatase</shortName>
            <ecNumber evidence="13">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="13">NTPase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">XTP/dITP diphosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ITPA</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Pyrophosphatase that hydrolyzes the non-canonical purine (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triphosphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triphosphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ITP + H2O = IMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:29400"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-hydroxy-dATP + H2O = N(6)-hydroxy-dAMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:83971"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:233529"/>
            <dbReference type="ChEBI" id="CHEBI:233530"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:83972"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>XTP + H2O = XMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:28343"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="13">Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQH3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011661"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011794.1">
        <property type="protein sequence ID" value="ENSXCOP00000011661.1"/>
        <property type="gene ID" value="ENSXCOG00000008802.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015399"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009204">
        <property type="term" value="P:deoxyribonucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.950.10:FF:000003">
        <property type="entry name" value="Inosine triphosphate pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03148">
        <property type="entry name" value="HAM1_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027502">
        <property type="entry name" value="ITPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="RdgB/HAM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067:SF9">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE/HAM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="13">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="72"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="148"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="176"/>
            <end position="177"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00093218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="205" mass="23063" checksum="50115829487388C7" modified="2018-12-05" version="1">VSCVLDVPFIFLTSFPPPHIFQVVHILGDQFPYKVVSKKIDLPEYQGEPDEVCIQKCKEAARQIDGPVIVEDTCLCFRALGGLPGPYIKWFLNQLKPTGLHTLLAGFEDKSAWALCTFAFSAGRGEPVQLFRGKVEGMIVEPRGPPDFGWDPCFEPDGYDKTYAELPKEVKNSISHRYRALAAMSEHFAKINRTSQESKKNKQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQH7</accession>
    <name>A0A3B5LQH7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Epsin 3a</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the epsin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQH7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014363"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014547.1">
        <property type="protein sequence ID" value="ENSXCOP00000014363.1"/>
        <property type="gene ID" value="ENSXCOG00000010892.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158217"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16990">
        <property type="entry name" value="ENTH_Epsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000002">
        <property type="entry name" value="epsin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF112">
        <property type="entry name" value="EPSIN 3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02809">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ENTH" evidence="9">
        <location>
            <begin position="12"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="138"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="271"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="473"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="3"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="473"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="498"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="529" mass="58077" checksum="EC43EFCE16E29383" modified="2018-12-05" version="1">MQSSSLRRQMKNMVNNYTEAEIKVREATSNDPWGPSSTLMAEIADLTYNVVAFTEVMGMILKRLNDHGKNWRHVYKALTLLDYLVKASSERVVKSCRDNIYTIQTLKDFQYIDRDGHDQGIHVREKAKQLAALLKDDGKLKSERSQAQKTKSRVGRGSAGHTVRQSQDDFNRCRSPSYNSSPSSRLDPDVEQAIPASSGEEELQLQLALAMSREESEKPPPTVGIDEQTQLQIAMKLSKEEAQKVKPVKRAPPPAMEMDEDAELQLALSLSKKEHQQEQRTRRGDDSDLQKALEESKREMEGKGGTAFMDLVDIFAVPTDAPPSDILHVTIYSSLLGFVYPTFLRCCLSLLLDGDLFDEAMDGGHASINGQDEDNSDLFNMSGLRDSLANTKPRACRTPESFLGPTAASLVDLNVLIPTNPPAKTTNPFLAGLSAPSAVNPFQAEQPKVSLNEMSSSFASPVSQSTSLPYSASLPLPTSHQGAAIPSSLTHPTPPGLELPVKLPEPLLPFSSASNEESQASQININPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQI2</accession>
    <name>A0A3B5LQI2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">T-box transcription factor T</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Brachyury protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Protein T</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027861911.1">
        <property type="nucleotide sequence ID" value="XM_028006110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQI2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014368"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014552.1">
        <property type="protein sequence ID" value="ENSXCOP00000014368.1"/>
        <property type="gene ID" value="ENSXCOG00000010896.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114137486"/>
    <dbReference type="KEGG" id="xco:114137486"/>
    <dbReference type="CTD" id="100004296"/>
    <dbReference type="GeneTree" id="ENSGT00940000157912"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001707">
        <property type="term" value="P:mesoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001756">
        <property type="term" value="P:somitogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20192">
        <property type="entry name" value="T-box_TBXT_TBX19-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.820:FF:000002">
        <property type="entry name" value="T-box transcription factor Brachyury"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002070">
        <property type="entry name" value="TF_Brachyury"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018186">
        <property type="entry name" value="TF_T-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF83">
        <property type="entry name" value="T-BOX TRANSCRIPTION FACTOR T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00938">
        <property type="entry name" value="BRACHYURY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00425">
        <property type="entry name" value="TBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01283">
        <property type="entry name" value="TBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01264">
        <property type="entry name" value="TBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="13">
        <location>
            <begin position="46"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="384"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="298"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="436" mass="47920" checksum="546275743AA8E44D" modified="2018-12-05" version="1">MATGAGECPSKAAQYRVDHLLSAVESELQAGSEKGDPTERDLKVSLDERELWQKFKDLTNEMIVTKNGRRMFPVLKANVSGLDPNAMYSFLLDFVSADNHRWKYVNGEWVPGGKPEPQTPSCVYIHPDSPNFGAHWMKAPVSFSKVKLTNKLNGGGQIMLNSLHKYEPRIHIVRVGGPRRMITSHSFPETQFIAVTAYQNEEITALKIKYNPFAKAFLDAKERNDHKDMREEANDNPQSGYSQLGSWFIHGTGTLCPPTSPHSQFGSPIALSPSHGCERYSSLRSHRSAPYSSPYAHRSNSPTGYSDNSSSCLSMLPSHDNWSSLQMPTHSSMMPMTHNSTCAANSSQYTSLWSVSNSPLTPVSQNGGLSSCLSSQFLRGSSSHYPSLPHSATAPPSVSPMYDSSSATEVQDPVQYEASPHGRLPSAWTPVTPPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQX0</accession>
    <name>A0A3B5LQX0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Complement component C8 beta chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Complement component 8 subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">C8B</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis. The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways. The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. C8B, together with C8A and C8G, inserts into the target membrane, but does not form pores by itself. During MAC assembly, associates with C5b, C6 and C7 to form the C5b8 intermediate complex that inserts into the target membrane and traverses the bilayer increasing membrane rigidity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="24">Heterotrimer of 3 chains: alpha (C8A), beta (C8B) and gamma (C8G); the alpha and gamma chains are disulfide bonded. Component of the membrane attack complex (MAC), composed of complement C5b, C6, C7, C8A, C8B, C8G and multiple copies of the pore-forming subunit C9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Target cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complement C6/C7/C8/C9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQX0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010184"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010303.1">
        <property type="protein sequence ID" value="ENSXCOP00000010184.1"/>
        <property type="gene ID" value="ENSXCOG00000007724.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160247"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005579">
        <property type="term" value="C:membrane attack complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044218">
        <property type="term" value="C:other organism cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006957">
        <property type="term" value="P:complement activation, alternative pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006958">
        <property type="term" value="P:complement activation, classical pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000001">
        <property type="entry name" value="semaphorin-5A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048831">
        <property type="entry name" value="C8A_B_C6_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001862">
        <property type="entry name" value="MAC_perforin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020863">
        <property type="entry name" value="MACPF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742">
        <property type="entry name" value="COMPLEMENT COMPONENT C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742:SF5">
        <property type="entry name" value="COMPLEMENT COMPONENT C8 BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21195">
        <property type="entry name" value="EGF_C8A_B_C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00764">
        <property type="entry name" value="COMPLEMENTC9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00279">
        <property type="entry name" value="MACPF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0179">Complement alternate pathway</keyword>
    <keyword evidence="15" id="KW-0180">Complement pathway</keyword>
    <keyword evidence="13" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="18 25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0391">Immunity</keyword>
    <keyword evidence="9" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0473">Membrane attack complex</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-1052">Target cell membrane</keyword>
    <keyword evidence="21" id="KW-1053">Target membrane</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="MACPF" evidence="26">
        <location>
            <begin position="111"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="93"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00031383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00093292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00093472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="545" mass="60812" checksum="E7E93936AABAEE12" modified="2018-12-05" version="1">MGNFVAEKRSTDNSLGLSTFKYLRWSSVWTVKSSYRYAKLEQPSQFGGEPCYLHGAQEERCEVPARYTCNSVPLCEGFLCTQTGRCVHRTLQCNGEDDCGDMSDEVGCTKTSKPCRVEAEEYWGIENLAKGINVLNSHLEGVVLDNRYYGGGCLPHYIQDVPFRKPFNLQQYTLQTRGSYDFTLQSFESYSDYMDHTFRERMTQTSFSIGFSVPGVFSFGFNYDSSKYSKSVSKIRRASGVTNSFIRAKAELELAQYILKSDGLVLHPEFLQRLRSLPQAYVYGEYRQIYKDYGTHYITEAALGGDFEHTTILNKEKLEKSDYNLDQYKSCLQIGFKIGVTIEGVPVSLGLGGGSCSGLLNEMGEDTKNGAMVEDFVAVVRGGNSESITALLSQKLPSEQLMRLWGEGVRYNPDFIRMTTKPLYELVTSRDFVQADSLKRNLKRALSEYLAETSACRCAPCHNNGVAVLKGTRCECVCPTGYTGRSCEITSRQKVLGIDGGWSCWGAWSSCSGSQMTRSRQCSNPAPSSGGLPCRGLQQEATECF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LR17</accession>
    <name>A0A3B5LR17_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Furry homolog b (Drosophila)</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LR17"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013240"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013404.1">
        <property type="protein sequence ID" value="ENSXCOP00000013240.1"/>
        <property type="gene ID" value="ENSXCOG00000010031.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00610000086058"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025481">
        <property type="entry name" value="Cell_Morphogen_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045842">
        <property type="entry name" value="Fry_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039867">
        <property type="entry name" value="Furry/Tao3/Mor2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295">
        <property type="entry name" value="FURRY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295:SF29">
        <property type="entry name" value="PROTEIN FURRY HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19421">
        <property type="entry name" value="Fry_C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14225">
        <property type="entry name" value="MOR2-PAG1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cell morphogenesis protein C-terminal" evidence="2">
        <location>
            <begin position="282"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Protein furry C-terminal" evidence="3">
        <location>
            <begin position="560"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="Protein furry C-terminal" evidence="3">
        <location>
            <begin position="799"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="domain" description="Protein furry C-terminal" evidence="3">
        <location>
            <begin position="1025"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="672"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="740"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1111" mass="123433" checksum="392F69F2422CF580" modified="2018-12-05" version="1">VVDSGLSSTSNSSSASLGGGSTGGSVGNFTVLFLLRAFGPLWAHEDITPKNPNSKSTDQLSNFLRHVVSVFKESKSDFHLEQQLSDVALQTALCSSSRHYAGRSFQIFRALKQPINNHAVSDLVSRLVEVVGEHGDEVQGYVMEVLLTLEAVVVNLAECLKNSDLMAALTRTSSPDFVTSDKLMNRKSTGQLNFPGPGFAGLSSQRHQRSYSVPKKFGECGNVLSDPPRSATLDRIQACNSHGLLSRVARTPGSCTSSTNRIDPSALSDPSHVSHPSSILATVFWVAVSLMESDFEFEYQMSLRLVHKLLSKVPLDRAENRERLEKLQAQLRWSGFSGIQQLLLKGFTSQTTSDLTLQLFCQLTPVSRVPVVDSSQSIGFPLNVLCLLPHLVQNFSHPTQFCKESAERIAQVCLEEKNTKLSHLAHVMTLYKTRSYTRDPFSWVSVVCRYLHEAFSDITLNMVTYMAELLDKGIPNMQLPLLQIIYCLLSHMDLTAVQVKQFNGDVMKTIEKFVHTVHWKDALNILKLVVSRSASLVHPSYIHSQGDFSNLEVSRVWDGSAKALPGKTLDFTFDISETPVIGRRFDGLQGSGGREGKARAMAVTRSTSSTSSGSNSNTILVPVSWRRPQSSQKRTREKLVNVLSLCGQEVGLTKNPSVIFSSCGDLDMMEVRESGVSSEEGGTREDTLDDTASEQQFRVFRDFDFLDVELEDGEGETVDNFNWGVRRRSLDSTELGDLLEESQHSGSTPSLGHEDPHDSDESSEEEESSTSQSLSHSQLVCSLRFYTKSMFPLELCGEDEDTQAQDDDLRDDVDDLEDLGFPPPPSPFFSAILAAFQPTVCDDAEEAWRCHVSQLVTDSDGSCAVYTFQVFSSLFKNIQGKFCTLTNDVATYLGEGLRGIGSKFLKSSRMLTTCSDCPTIYIDADTIMSYGLLDKMKFSALELQEYLDTYNSKEEAAVSWLRNCKDTFPRCPVENQLELCQRLYKLHFQLLLLFQSYCSLIGQVHAISSVPELCALPLNLFDLLIRRQWPSGVFGGSSETEVQTLLNIYFRHQTLGQTGTIALVGSRQDLSLICSKLLELNGEIRDMIRRAQGYRVRNLTVTAFFSKVMIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSR8</accession>
    <name>A0A3B5LSR8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IF rod domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSR8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015053"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015242.1">
        <property type="protein sequence ID" value="ENSXCOP00000015053.1"/>
        <property type="gene ID" value="ENSXCOG00000011408.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159655"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052857">
        <property type="entry name" value="IF_Keratin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47082">
        <property type="entry name" value="KERATIN-LIKE PROTEIN KRT222"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47082:SF1">
        <property type="entry name" value="KERATIN-LIKE PROTEIN KRT222"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="5">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="117"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="273" mass="30920" checksum="09B6EFF52B6FD62D" modified="2018-12-05" version="1">MELTQLFDQIRAQCDQSRPAGLAERHRVLGTASDPEEAAWAQVSLGGAALKEARAELAEARKQWRSLQVEIETLHALEKGLECSLQHTQELYTSQLHDLSQVIVGLESELEQVRSGLATQRQRHSQLLNTKMRLEREITIYRQLLEREEGRTLSQPYLLMVCHGCLSIEPEQDKDLPLSTVKTQEILQGNVVRESAEGHGTIETEKIDKVIKQWEGSFFRGNPKLRKKSVSLRFDLHMAAADEGCGQTKQDSLPDVEVRLIMKRSRSISTITQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTC0</accession>
    <name>A0A3B5LTC0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">THAP domain containing 12b</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027900558.1">
        <property type="nucleotide sequence ID" value="XM_028044757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTC0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014075"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014250.1">
        <property type="protein sequence ID" value="ENSXCOP00000014075.1"/>
        <property type="gene ID" value="ENSXCOG00000010652.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114161468"/>
    <dbReference type="KEGG" id="xco:114161468"/>
    <dbReference type="CTD" id="436953"/>
    <dbReference type="GeneTree" id="ENSGT00530000063516"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052958">
        <property type="entry name" value="IFN-induced_PKR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46289">
        <property type="entry name" value="52 KDA REPRESSOR OF THE INHIBITOR OF THE PROTEIN KINASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46289:SF13">
        <property type="entry name" value="52 KDA REPRESSOR OF THE INHIBITOR OF THE PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="749" mass="85546" checksum="85FBF5A98B7F33E6" modified="2018-12-05" version="1">MPNFCAAPNCTRKSTQSDLAFFRFPRDPERCRIWVENCRRADLEAKTADQLNKHYRLCAKHFDPAMVCKTSPYRTVLKDTAIPTIFDLTSHLKNPYTRHRKQIKELTDEDIQRIKERRLASAEQTIKKEDLSNMVDNEPQLSADEKEYRDYLRSLFEILFMLAKQAIPLTTGRVSEEELKSNNFQAVIDYCMNAGDEALRKRFEVTAVNSEYLSAAQLNQLLDVCENTVREEMLMEVRESRFYSLVTGDLVEFANSKHLPVFLRFVNQQNVLREEFVDFLPFVDGDEAALVESLEALLVDRWGLSMEDCRGQAHKATGTSTTKMKAVAVLLMEKYPLALHMPCSHLALNIHLANNLPFPNVQIVMENLRRITSFFKTPHTQDELEKAISIQFQKNEEKRNALKKMCSSGWTEQHNIFDVLLDILPSLLLCMDSIRDNADRKFSSSVTADAYSIGESIADFEVIVTIVILKNVLTFTRAFGRNLQGETLDVFSAASSLTAVLYSLNEVNDNIDVYHEFWYSEAISLAALMQIPVSVPRLFLRKQRAADLGEIQAETYFKEYVTVPVIRGIMQEVEDMFCDNNLRALKCLSLVPAVMGHMKFNTTEENFAEVYSGDLPNPDTLPAELHCWRIKWKHRGKEVRLPTTIHETLQLPDVKFFPNVDAFLKVLSTMPVLKVEKGTGDAATERLQAYVGSVPAKHLNKSLAMLHINTHVKHDLDVMVDKYCRLYPEDDPEAEAEDVGDDVIVTKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTR8</accession>
    <name>A0A3B5LTR8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Agouti-signaling protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Agouti switch protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTR8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015403"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015595.1">
        <property type="protein sequence ID" value="ENSXCOP00000015403.1"/>
        <property type="gene ID" value="ENSXCOG00000011665.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154258"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031779">
        <property type="term" value="F:melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032438">
        <property type="term" value="P:melanosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.760.10">
        <property type="entry name" value="Agouti domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007733">
        <property type="entry name" value="Agouti"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027300">
        <property type="entry name" value="Agouti_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036836">
        <property type="entry name" value="Agouti_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16551">
        <property type="entry name" value="AGOUTI RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16551:SF1">
        <property type="entry name" value="AGOUTI-SIGNALING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05039">
        <property type="entry name" value="Agouti"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00792">
        <property type="entry name" value="Agouti"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57055">
        <property type="entry name" value="Agouti-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS60024">
        <property type="entry name" value="AGOUTI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51150">
        <property type="entry name" value="AGOUTI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0960">Knottin</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017481895" description="Agouti-signaling protein" evidence="11">
        <location>
            <begin position="21"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Agouti" evidence="12">
        <location>
            <begin position="86"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="86"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="93"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="109"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="125" mass="13674" checksum="E07C35EAF873B34F" modified="2018-12-05" version="1">MQAFLPLGCCVLALTQCCLGSAHMIPDDRLSTNRVVASNALSHSLQIDPPPVVIVELNKSTKKKEKGKKQKKVGFARKRPPPPPNCIPLWGSCKAPGNVCCDFCAFCQCRLFKTVCLCRMGNPRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUC8</accession>
    <name>A0A3B5LUC8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">SEC22 homolog B, vesicle trafficking protein b</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus</location>
            <location evidence="9">cis-Golgi network membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Melanosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUC8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012916"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013077.1">
        <property type="protein sequence ID" value="ENSXCOP00000012916.1"/>
        <property type="gene ID" value="ENSXCOG00000009775.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156349"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.50:FF:000004">
        <property type="entry name" value="vesicle-trafficking protein SEC22b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044565">
        <property type="entry name" value="Sec22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45837">
        <property type="entry name" value="VESICLE-TRAFFICKING PROTEIN SEC22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="11">
        <location>
            <begin position="6"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="183" mass="20977" checksum="0345C264933289E0" modified="2018-12-05" version="1">MLLLTMIARLADGLPLAASMQEDEQVGRDLQQYQTQAKQLFRKLNEQSPTRCTLEAGTMAFHYFIEQGVCYLVLSEASFSKKLAFAYLEDLQAEFHEQHGKKVPTVSRPYSFIEKSLLLLCTHRLLPHLPRTALDSKASNLSSLSKKYRSDAKYLNTRSTYAKLAAGGVFFIMLIVYIRFWWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LV15</accession>
    <name>A0A3B5LV15_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cystatin A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CSTA</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cystatin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LV15"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013312.1">
        <property type="protein sequence ID" value="ENSXCOP00000013151.1"/>
        <property type="gene ID" value="ENSXCOG00000009964.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155717"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018073">
        <property type="entry name" value="Prot_inh_cystat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001713">
        <property type="entry name" value="Prot_inh_stefin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11414:SF21">
        <property type="entry name" value="CYSTATIN 14A, TANDEM DUPLICATE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11414">
        <property type="entry name" value="CYSTATIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00287">
        <property type="entry name" value="CYSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="domain" description="Cystatin" evidence="6">
        <location>
            <begin position="31"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="103" mass="11666" checksum="CE0EB662FB16F1D8" modified="2018-12-05" version="1">MSKGILVGGWEDPRPADGEISMLSSWVKATTNRKFNEFKAVEYRTQVVAGVNYVIKVQVGENNFIHLQIFRSPLMGNTIEIKFEGAQDGYKRDDPLEPFTPFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVZ4</accession>
    <name>A0A3B5LVZ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVZ4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015045"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015234.1">
        <property type="protein sequence ID" value="ENSXCOP00000015045.1"/>
        <property type="gene ID" value="ENSXCOG00000011399.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155134"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048788">
        <property type="term" value="C:cytoskeleton of presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048791">
        <property type="term" value="P:calcium ion-regulated exocytosis of neurotransmitter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050806">
        <property type="term" value="P:positive regulation of synaptic transmission"/>
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    </dbReference>
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        <property type="term" value="P:regulation of membrane potential"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048167">
        <property type="term" value="P:regulation of synaptic plasticity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000300">
        <property type="term" value="P:regulation of synaptic vesicle exocytosis"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="C2B_RIM1alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000001">
        <property type="entry name" value="Regulating synaptic membrane exocytosis 3, isoform CRA_a"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039032">
        <property type="entry name" value="Rim-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12157">
        <property type="entry name" value="REGULATING SYNAPTIC MEMBRANE EXOCYTOSIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12157:SF18">
        <property type="entry name" value="REGULATING SYNAPTIC MEMBRANE EXOCYTOSIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
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    </dbReference>
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        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="164"/>
            <end position="282"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="121"/>
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        <location>
            <begin position="12"/>
            <end position="28"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="82"/>
            <end position="92"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="106"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="318" mass="34568" checksum="E7D891D2DE330D08" modified="2018-12-05" version="1">MFIQSPLPYSRQMRSSMGRNMMKSSSVSGEIYTQERTDGSQSDTALGTVSGGSKKRRSSISARFVAIVGKNRSRSTSQISDPEGKTKREKGIQRSTETGMAVELSRNMSRQPSRESNNGSMNSCNSEGNFCVSLNLGASSQFSDFLDGLGPAQLVGRQTLATPAIGDIQIGMIEKKGQLEVEVIRARGLVQKPGSKSLPAPYVKVYLLNNGAYVAKKKTKIARKTLDPLYQQALLFEESPQGKVLQVIVWGDYGRMDHKSFMGVAQILLDELDLSNTVIGWYKLFPPSSLVDPTLASLTRRASQSSLDSSSGPPGVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LW18</accession>
    <name>A0A3B5LW18_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EVA1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EVA1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LW18"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016364.1">
        <property type="protein sequence ID" value="ENSXCOP00000016158.1"/>
        <property type="gene ID" value="ENSXCOG00000012210.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154096"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052461">
        <property type="entry name" value="EVA1_A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039500">
        <property type="entry name" value="EVA1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48422:SF1">
        <property type="entry name" value="PROTEIN EVA-1 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48422">
        <property type="entry name" value="PROTEIN EVA-1 HOMOLOG B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14851">
        <property type="entry name" value="FAM176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="EVA1" evidence="7">
        <location>
            <begin position="19"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="164" mass="18211" checksum="D01B1E88FAE483FC" modified="2018-12-05" version="1">MSTPTPGSPNSEVKMGSQEMAMLSNLLAAYTFIADQPERTALVFLGGVCVGLLVTLCAIVFQIHCRADCHYDGNPDNTGAVVVASGVTGNTGDCEPEDWDDTSDLSARRRRRFERALLNATMFTSSEELDRAQRLEERERVLREIWMNGQPDISTVTQSLNRYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZ67</accession>
    <name>A0A3B5LZ67_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Calcium homeostasis modulator 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CALHM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZ67"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016452"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016659.1">
        <property type="protein sequence ID" value="ENSXCOP00000016452.1"/>
        <property type="gene ID" value="ENSXCOG00000012414.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234610"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029569">
        <property type="entry name" value="CALHM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32261">
        <property type="entry name" value="CALCIUM HOMEOSTASIS MODULATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32261:SF2">
        <property type="entry name" value="CALCIUM HOMEOSTASIS MODULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14798">
        <property type="entry name" value="Ca_hom_mod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="256"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="351" mass="40656" checksum="59B5DBD617634055" modified="2018-12-05" version="1">MDKFRMMFQFLQSNQESFMNGICGIMALASAQMYSAFEFSCPCMPEYNYTYGIGLLIIPPIWFFLLGFVLNNNVSVLAEEWKRPTGSRTKDPTILRYMFCSITQRSLIAPALWVSVTLMDGKSFLCAFSINLDIEKFGNSSFVKGMSETEKIRLLARIPCKDLFEHQEIRVAATRYIKCISQACGWIFLLMMTFTAFLIRAIRPCFTQAAFLKTKYWSHYIDIERKMFDETCKEHAKSFAKVCIHQYFENISGEMQSLHRHRSGKDENGDEDEDKRSDEDKLLGIRAQDDMNKLLWNWHTCKPALALRKDQMDGENNSKLNGKINEGFNGFANGHAHETKKKEWAVYYSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZD2</accession>
    <name>A0A3B5LZD2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZD2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016325"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016531.1">
        <property type="protein sequence ID" value="ENSXCOP00000016325.1"/>
        <property type="gene ID" value="ENSXCOG00000012334.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000069547"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF35">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017274122" description="Fibronectin type-III domain-containing protein" evidence="11">
        <location>
            <begin position="26"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="117"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="388"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="391"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="536" mass="59149" checksum="87AF4019DE16A9B1" modified="2018-12-05" version="1">YFSARLSMASFQLLLFLGYFATAQAAIGIKNHHFNLIRFVDLDCFNDYDNIFCRLGVKECSQYTLIVDDIYDKYNRSLEKCNSEQCCCCSTTFDPISGTRSSLEKLKSVSLFFYTVKPKTPTIVSVDKTEGIFTVYWKTHMSVLLDKPTAEVTISKIGTQWKVRPNILVLITNFQINSQDLEPDTNYTVSVRSYTLYSHNFSDISEEFKFETSSAPNSLLLWIIISLSIFGVILIVSIYICFVNVSGNSSGGSSDLCYGQTEPLEPKDDISDTINKAVLECLSKHFTFSGPPPGPQDKMVPLFTSKKPSSVSSGIVNRSYFMSLSKSLDQSKEDSSTVEFPDNSINSCKSDTVDCPDQQIPAGLLSAQKDISSVVQVDMSYQQFKADANQTPLSENSSLSSTFSGTTTATSSGFDSSNASSFKRFDEANPDFLKMLLSSSLPSGTRHCPVVADDYKPFPNKVEQPGVLPSENQSIDHQGLNVIQGEKVLQTPQPCFIPDLKKWNGPSPSVLQIPHLTLLSSDKSAPVIIVGGYKCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M142</accession>
    <name>A0A3B5M142_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Neurotransmitter-gated ion-channel transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M142"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017127"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017347.1">
        <property type="protein sequence ID" value="ENSXCOP00000017127.1"/>
        <property type="gene ID" value="ENSXCOG00000012914.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154713"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0868">Chloride</keyword>
    <keyword evidence="1" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="1" id="KW-0407">Ion channel</keyword>
    <keyword evidence="1" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="5">
        <location>
            <begin position="7"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="207" mass="23458" checksum="38268F253B69E96A" modified="2018-12-05" version="1">IKNVKCTILKNVFFWISLDIYIIAINIFWITTVLTMTTINTHLRETLPKIPYVKAIDMYLMGCFVFVFLALLEYAFVNYIFFGRGPQMQKKLALKAMKANNERGRFDQPKVDSHGNILLTSLEIHNEVGRNEVTTTLSETHNSTSMTFDNSGVQYRKPSKRKAAGRHGLRRTICTACLRGLQRVGVTPPVRSPPSSATRGSSCLMEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M195</accession>
    <name>A0A3B5M195_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tr-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M195"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015371"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015562.1">
        <property type="protein sequence ID" value="ENSXCOP00000015371.1"/>
        <property type="gene ID" value="ENSXCOG00000011639.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074806"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01885">
        <property type="entry name" value="EF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000732">
        <property type="entry name" value="Elongation factor like GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF3">
        <property type="entry name" value="ELONGATION FACTOR-LIKE GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="1">
        <location>
            <begin position="17"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="294" mass="32823" checksum="EA9DD99BD79C689D" modified="2018-12-05" version="1">MGVTGLDKIKTLQRNPANIRNLCILAHVDHGKTTLADCLVANNGIISSRLAGKLRYLDSREDEQVRGITMKSSAISLHYSTGNQEYLLNLIDSPGHVDFSSEVSTAVRLCDGAIVVVDAVEGVCPQTQVVLRQAWLENIRPVLVINKMDRLIMELKLTALEAYTHLQKILEQVNAVTGTLFTSKVLEERAEKVKEEVNRDKDPSGDQVYDWSAGLEDADDSHLYFSPDQGNVVFASAIDGWGFSIQHFAHIYSQKMGIRTEVLLKTLWGDFYLNTKIQRIMKGLYLVVQLCCVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2R3</accession>
    <name>A0A3B5M2R3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Cytosolic purine 5'-nucleotidase</fullName>
            <ecNumber evidence="21">2.7.1.77</ecNumber>
            <ecNumber evidence="4">3.1.3.5</ecNumber>
            <ecNumber evidence="5">3.1.3.99</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Cytosolic nucleoside phosphotransferase 5'N</fullName>
        </alternativeName>
    </protein>
    <organism evidence="35 36">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="35" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="35" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Broad specificity cytosolic 5'-nucleotidase that catalyzes the dephosphorylation of 6-hydroxypurine nucleoside 5'-monophosphates. In addition, possesses a phosphotransferase activity by which it can transfer a phosphate from a donor nucleoside monophosphate to an acceptor nucleoside, preferably inosine, deoxyinosine and guanosine. Has the highest activities for IMP and GMP followed by dIMP, dGMP and XMP. Could also catalyze the transfer of phosphates from pyrimidine monophosphates but with lower efficiency. Through these activities regulates the purine nucleoside/nucleotide pools within the cell.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>GMP + H2O = guanosine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27714"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16750"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:27715"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>IMP + H2O = inosine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27718"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="3.1.3.99"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:27719"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>XMP + H2O = xanthosine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:28530"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18107"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:28531"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>a 2'-deoxyribonucleoside + a ribonucleoside 5'-phosphate = a ribonucleoside + a 2'-deoxyribonucleoside 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19961"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="2.7.1.77"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:12485"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>dGMP + H2O = 2'-deoxyguanosine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:29379"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17172"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57673"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:29380"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>dGMP + inosine = 2'-deoxyguanosine + IMP</text>
            <dbReference type="Rhea" id="RHEA:69580"/>
            <dbReference type="ChEBI" id="CHEBI:17172"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:57673"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>dIMP + H2O = 2'-deoxyinosine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:29383"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28997"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:29384"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>dIMP + inosine = 2'-deoxyinosine + IMP</text>
            <dbReference type="Rhea" id="RHEA:69572"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:28997"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>inosine + AMP = IMP + adenosine</text>
            <dbReference type="Rhea" id="RHEA:69596"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>inosine + CMP = cytidine + IMP</text>
            <dbReference type="Rhea" id="RHEA:69592"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>inosine + GMP = guanosine + IMP</text>
            <dbReference type="Rhea" id="RHEA:69584"/>
            <dbReference type="ChEBI" id="CHEBI:16750"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>inosine + UMP = uridine + IMP</text>
            <dbReference type="Rhea" id="RHEA:69588"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="33">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="33">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 5'(3')-deoxyribonucleotidase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.77" evidence="21"/>
    <dbReference type="EC" id="3.1.3.5" evidence="4"/>
    <dbReference type="EC" id="3.1.3.99" evidence="5"/>
    <dbReference type="RefSeq" id="XP_027861785.1">
        <property type="nucleotide sequence ID" value="XM_028005984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M2R3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018393"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018625.1">
        <property type="protein sequence ID" value="ENSXCOP00000018393.1"/>
        <property type="gene ID" value="ENSXCOG00000013852.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114137422"/>
    <dbReference type="CTD" id="324845"/>
    <dbReference type="GeneTree" id="ENSGT00940000163289"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050146">
        <property type="term" value="F:nucleoside phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046037">
        <property type="term" value="P:GMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07522">
        <property type="entry name" value="HAD_cN-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000021">
        <property type="entry name" value="NT5C2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008380">
        <property type="entry name" value="HAD-SF_hydro_IG_5-nucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016695">
        <property type="entry name" value="Pur_nucleotidase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02244">
        <property type="entry name" value="HAD-IG-Ncltidse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103">
        <property type="entry name" value="5'-NUCLEOTIDASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103:SF17">
        <property type="entry name" value="CYTOSOLIC PURINE 5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05761">
        <property type="entry name" value="5_nucleotid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017434">
        <property type="entry name" value="Purine_5'-nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 33" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 33" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="34">
        <location>
            <begin position="532"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="34">
        <location>
            <begin position="550"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="32">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="32">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00038866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00040391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00042328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00046009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00047560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00048038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00048127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00048155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00048156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00048783"/>
        </source>
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    <evidence type="ECO:0000256" key="33">
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            <dbReference type="PIRSR" id="PIRSR017434-2"/>
        </source>
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    <evidence type="ECO:0000256" key="34">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="561" mass="64897" checksum="0AE0C582032020D9" modified="2018-12-05" version="1">MTTSWSNRLQNCAELPANMDGIAMKKYRREAHHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFDLTVERLVSIGYPQELLNFVYDPSFPTRGLVFDTMFGNLLKVDAYGNILVCAHGFNFLRGPEIREMYPNKFIQRGDTDRFYILNTLFNLPETYLFACLVDFFTNCPRYASCESGFKDGDLFMSYKSMFQDVRDAVDWVHFKGSLKEKTVENLEKYVVKDPKLPLLLSRMNEVAKVFLATNSDYKYTEKIMTYLFDFPHGPKPGTPHRPWKSYFGLILVDARKPVFFAEGTVLRQVDTTTGRLKIGTYTGQLQHGIVYSGGSSDIVCDLLGAKGKDIVYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSAIFEELQSLDCFLADLYKHLDSSSNERPDISSLQRRIKKVTHDMDMCYGMMGSLLRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHSHVNLIDTESPLATRNRRDFDVKEMEIKRHQLTRSISEIQPPHLFPQTPQEITHCHDEDDDEEEEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4Q6</accession>
    <name>A0A3B5M4Q6_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Ecdysoneless cell cycle regulator</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="primary">ECD</name>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4Q6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016406"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016613.1">
        <property type="protein sequence ID" value="ENSXCOP00000016406.1"/>
        <property type="gene ID" value="ENSXCOG00000012384.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010770">
        <property type="entry name" value="Ecd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13060:SF0">
        <property type="entry name" value="PROTEIN ECDYSONELESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13060">
        <property type="entry name" value="SGT1 PROTEIN HSGT1 SUPPRESSOR OF GCR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07093">
        <property type="entry name" value="SGT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="441"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="516"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="518"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="655" mass="72784" checksum="3D93E1DA0AB3BF06" modified="2018-12-05" version="1">MDALQRRSIPEDMVQYKLFPVQTDMASQQTEERLKHLTEEMLAKVAQLLVQYIWQDQPFNLKYHPEEGDVPAHIGGSTQYGDNIEDEWFIVYLLLHLTELFPELAARVEDNDGDFLLIEAADHLPKWLNPDTSENRVFIYRGELHILPCPSKSSSIGFSLDVVPSLAQALALLSAHPEACQASPKIRSALKKRLQGYPEKIKANLHRAHCFIPAGVASVLAQRPDLVAPAVSAFYLRDPVDLQACRSFRTFPPDTRVLASVTFTRCLYAQLQQQQFTPDRRSGFDLPPRSHPRYKAHELGMKLAHGFEILCSKSRLPSSEADASVNCNPQWRNFLESLKKNGYFRGELEGSAVYKELLRSAEIFFKQSVASKSSSISAGEEILQLLHSSPLNLVEFKKLESQLPPEDSDSWLEVTAQDLEQLLQERSSRVADVDCEGFSSAKQKRPVEEAAGKEDEEDTNVEDAGYRLVAVSQGMKDFLNAMSSHEGAEAPCLQTQPFSFDPGSMANALDKLLGGKEEELDSDDLDDDYDDDDEEEEEEEVVNATEEGSSGPAEMSAAETLNSLKSYMDQMDQELQSTNVGKSFSQTVNTSFYVFIQNLAGLKCTQAIEIQPLDIDVNLVANLLESLSSQSGLAGPASNLLQSLGIQLPPNSDPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M573</accession>
    <name>A0A3B5M573_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Neuroplastin b</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
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    <dbReference type="AlphaFoldDB" id="A0A3B5M573"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018525"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018758.1">
        <property type="protein sequence ID" value="ENSXCOP00000018525.1"/>
        <property type="gene ID" value="ENSXCOG00000013943.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:cell-cell adhesion mediator activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:axon guidance"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:dendrite self-avoidance"/>
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    </dbReference>
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        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
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    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Neuroplastin b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075">
        <property type="entry name" value="BASIGIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075:SF108">
        <property type="entry name" value="NEUROPLASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01856">
        <property type="entry name" value="BASIGIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017199302" evidence="9">
        <location>
            <begin position="24"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="27"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="116"/>
            <end position="212"/>
        </location>
    </feature>
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        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
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        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="262" mass="29569" checksum="709C72CF6AD55BB9" modified="2018-12-05" version="1">MHPIAVMLAVVLLGNLMLPFASAQNEPKINASDQIVLPLDGPVKSVILHCNLTAAHTAHKDSFWMKNGREIPNTRGEERTRSISKPRADDSGEYMCVYTFDKAPNANATIEVKAKPYVTGHKRSENKNEGENAMMYCKSVGYPHPTWTWRKVDGTSYKDIDNSTGRFFITNRDNYTEINILNLDITTDPGIYECNATNVIGNTAETTILRVRSHLAPLWPFLGVLAEIIILVVIIVVYEKRKRKTNSTNNHKDKNIRQRNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5V5</accession>
    <name>A0A3B5M5V5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="10">Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1). Interacts with CCDC32; the interaction is direct and mediates association of CCDC32 with adaptor protein complex 2 (AP-2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <location evidence="2">Coated pit</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M5V5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016796"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017013.1">
        <property type="protein sequence ID" value="ENSXCOP00000016796.1"/>
        <property type="gene ID" value="ENSXCOG00000012673.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00970000193421"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046959">
        <property type="term" value="P:habituation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036343">
        <property type="term" value="P:psychomotor behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14833">
        <property type="entry name" value="AP2_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000004">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027156">
        <property type="entry name" value="APS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0168">Coated pit</keyword>
    <keyword evidence="6" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="12">
        <location>
            <begin position="10"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="150" mass="17908" checksum="0CD3C9914FA11A54" modified="2018-12-05" version="1">MGFVVLKMKIRFILIQNRAGKTRLAKWYMQFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNFKIIYRRYAGLYFCICVDVTDNNLAYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFLAGEIRETSQTKVLKQLLMLQSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M713</accession>
    <name>A0A3B5M713_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Muscle RAS oncogene homolog a</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M713"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019265"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019503.1">
        <property type="protein sequence ID" value="ENSXCOP00000019265.1"/>
        <property type="gene ID" value="ENSXCOG00000014487.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156353"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="132" mass="15517" checksum="4A18B24067457E27" modified="2018-12-05" version="1">MREQYMRTGDGFLIVFSVTDKASFEHVDRFHQLILRVKDREAFPMVLVANKVDLVHLRKVTSDQGQEMAAKHNITYIETSAKDPPMNVDKAFHELVRVIRQQVPERNQKKKKKMKWRAERSTGSHRFHCAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8E1</accession>
    <name>A0A3B5M8E1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ubiquitin carboxyl-terminal hydrolase 34</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Deubiquitinating enzyme 34</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ubiquitin thioesterase 34</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Ubiquitin-specific-processing protease 34</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">USP34</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Ubiquitin hydrolase that can remove conjugated ubiquitin from AXIN1 and AXIN2, thereby acting as a regulator of Wnt signaling pathway. Acts as an activator of the Wnt signaling pathway downstream of the beta-catenin destruction complex by deubiquitinating and stabilizing AXIN1 and AXIN2, leading to promote nuclear accumulation of AXIN1 and AXIN2 and positively regulate beta-catenin (CTNBB1)-mediated transcription. Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with AXIN1 and AXIN2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016049"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016250.1">
        <property type="protein sequence ID" value="ENSXCOP00000016049.1"/>
        <property type="gene ID" value="ENSXCOG00000011941.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158659"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02659">
        <property type="entry name" value="peptidase_C19C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000014">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021905">
        <property type="entry name" value="DUF3517"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF827">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12030">
        <property type="entry name" value="DUF3517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="15">
        <location>
            <begin position="1430"/>
            <end position="1774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="993"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="2651"/>
            <end position="2745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1002"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="2728"/>
            <end position="2742"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="2820" mass="322272" checksum="90A37CB18A1559DD" modified="2018-12-05" version="1">VLENCSLSDADGSEGGFQLKKEHALRVLGYISSWSQRQCLCCFKEYKHLEVFNQLVYALINLVITQINSLRNQLCKSAPGSLPHESLNGENAAGGGGDNLDPFSSWTTEEKEKLLLCAAKIFQIQFPLYTAYKHNTHPTIEDISAHESNILGSFCDMNDVEVPLHLLRYVCLFCGKHGLSLMKECFELGTPESLPFPIAHAFITIVSNIRIWLHIPAVMQHIIPFRTYVIRSPACRRATQRQTGQTTMHSHIPSLFVNFNKVYLWRYLRSFSFLQLVVGVGLSAAVMIDSSTSPMVEDMLSADDVSCSSSQVSAKSEKNMADFDGEESGCEEELVQINSHAELSSHLQQHLPNLASIYHEHLVQGPAVHKHQYSSHAVTDINLDNVCKKGNTLLWDLVQDEDAIHLSEGLINEAEKLLCSLVCWFTDRQIRMRFIEGCLDNLAHHRSVVVSLRLLPKLFGTFQQFGSSYDTHWITMWAEKELHMMKLFFDNLQHYIQEVREHRHKFALYSHSAEVQVRLQFLTCVFSTLGSPDHFRLSLEQVDILWHCLVEDAECYDDALHWFLNQVRSKDQHAMGMETYKHLFLEKMPQLKPETISMTGLNLFQHLCNLARLATSTLDNASNCELCGMDQLWGIALRAQSADISRAAIQYINSYYINAGKTGLEKEQEFIRKCMESLLMASANLEKDAHSSLTSIERGLLMLKTHLEAFRRRFAYHLRQWQIEGTGISSHLKALSDKQSLPLRIVCQPAGLPDKMTIEMYPSDQVADLRAEVTHWYENLQKEQMNQQAQLQEFGQSSRQQGDFPGGLMGPVRMISSGHELTTDYDEKTLHELGFKDMQMVFVSLGAPRRERKGEGVQLPASCLPPPQKEHIPMLLLLQEPHLTTLFDLLEMLACFKPPSPNTEKLQESPESARCEELHLHAENLSRRVWELLMLLPTCPKMLQAFQNISDETSGESLCWKELLRIKSPHKLLYALEIIEALGKPNRRIHRESTGSYSDLYPDSDDSSEDQIENSKNTWSCKFVSSGGLQLLLEIFNSGILEPKDQESWTVWQLDCLACLLKLICQFAVDPADLDLAYHDVFSWSGLAESQRKRAWPGKSRKSTVEHGKGLHIPRLTEVFLSLVQGTNLIQRLINVAYTYDNLAHRVLKAQSDHRSRHEVTHYSMWLLVSWAHCSSTVKSSLADSDHLHDWLKKLTLLVPEPAVRHEACNGLYKLSLSGLEGGESINRSFLLLAASTLLKFLPDAQALKPLRVEDYEEEPLLRTGCKEYFWLLCKLIDNIHVKDASQTTLLDLDALARHLADCIRSREILDQQDGNIEDDGLTGLLRLATSVLKHKPPFKFSREGQEFLRDVYNLLFLLPSLADRAQPKCKSHSARAAAYDLLVEMVKGSVENYRLLHNWVMSQHMQSSHAPYKWDYWPHDDVRAECRFVGLTNLGATCYMASTIQQLYMIPEARQAVFTAKYSEEIKHKTTLLELQKMFTYLMESERKAYNPRPFCKTYTMDKQPLNTGEQKDMTEFFTDLITKIEEMSQELKNTVKTLFGGVITNNVVSLDCDHVSQTAEEFYTVRCQVADMKNIYESLDEVTIKDTLEGDNMYTCSQCGKKVRAEKRACFKKLPRILSFNTMRYTFNMVTMMKEKVNTHFSFPLRLNMTPYTEDFLMGKGERKEGFREEREQSVTESYEYDLIGVTVHTGTADGGHYYSFIRDIVNPHAYKNNKWYLFNDAEVKPFDSAQLASECFGGEMTTKTYDSVTDKFMDFSFEKTHSAYMLFYKRVELEEENGKDFSFDVSPDLLEWIWHDNMQFLQDKNIFEHTYFGFMWQLCSSIPSTLPDPKAVSLMTAKLSTSFVLETFIHSKEKPTMLQWIELLTKQFNNNQAACEWFLDRMADDNWWPMQILIKCPNQIVRQMFQRLCIHVIQRLRPVHAHLYLQPGMEDGSDDMDGPVEDIGSRSCVTRFVKTLLSIMEHGVKPHSKHLTEYFAFLYEFAKMGEEESQFLLSLQAISIMVHFYMGTKGPENPQVEVLSEEEAEEEDEEEDILSLAEEKYRPAALEKMIALIALLVEQSRSERHLTLSQSDMAALTGGKGFPFLFQHIRDGINIRQTCNLIFSLCRYNNRLAEHIVSMLFTSIAKLTPEAANPFFKLLTMLMEFAGGPPGMPSFASYILQRIWEVIEYNPSQCLDWLAVQTPRNKLAHSWVLQNMENWVERFLLAHNYPRVRTCEKPSVITQLINIKVIKTIEAHQMEENSPSEPISCALLSPSAFRVLLENDEDRLLVVFNRGLILMTESFNTLHMMYHEATACHVTGDLVELLSIFLSVLKSTRPYLQRKDVKQALIQWQERIDFAHKLLTLLNSYSPPELRNACLDVLKELVLLSPHDFLHTLVPFLQHNHCTYHHSNIPMSFGPYLPCRENIKLMGAKNNIRPPRPELNMCLLPSLVESSKGKDEVYDRMLLDYFLSYHQFIHLLCRVAINCEKFTDTLVKLSVLIAYEGLPLHLALFPKLWTELCQSQSVLAKTCVKLLCDDPAFSEYIKCILMDERTFLNNNMVYSFLTCFLPKVPLQVLSGPSCSNLISVLVTNLLTEQSNLQPELALHRVELNKTSSLLNADLRSLVLLLSIQPPPSIDAALCPALQELLGRCRLCLQQRSSLELEAKDLKSKAEDEGATPVKRRRVSSDDDREALTPASTSDTETRDSSSLIDPGTEQDPPSPEPTPATSRNMDSALKEEKMEVSSSSSSSFSSSSSSSLLQDAGDGFVQVEEPEAPQSVKCLLYLVNRLSNLGKEDANERLRIMKLTNIFSTWRKRQHWEIRGESGRNIKTGPKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MB21</accession>
    <name>A0A3B5MB21_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22 24">E3 ubiquitin-protein ligase CBL</fullName>
            <ecNumber evidence="7 24">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6 24">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="24">The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="7 24"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MB21"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016904"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017121.1">
        <property type="protein sequence ID" value="ENSXCOP00000016904.1"/>
        <property type="gene ID" value="ENSXCOG00000012746.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155772"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046875">
        <property type="term" value="F:ephrin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001784">
        <property type="term" value="F:phosphotyrosine residue binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042059">
        <property type="term" value="P:negative regulation of epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902531">
        <property type="term" value="P:regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16708">
        <property type="entry name" value="RING-HC_Cbl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09920">
        <property type="entry name" value="SH2_Cbl-b_TKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14393">
        <property type="entry name" value="UBA_c-Cbl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000022">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000030">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.20:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000015">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000154">
        <property type="entry name" value="E3 ubiquitin-protein ligase CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.20">
        <property type="entry name" value="Adaptor protein Cbl, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024162">
        <property type="entry name" value="Adaptor_Cbl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014741">
        <property type="entry name" value="Adaptor_Cbl_EF_hand-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036537">
        <property type="entry name" value="Adaptor_Cbl_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003153">
        <property type="entry name" value="Adaptor_Cbl_N_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014742">
        <property type="entry name" value="Adaptor_Cbl_SH2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024159">
        <property type="entry name" value="Cbl_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23007">
        <property type="entry name" value="CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23007:SF5">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE CBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02262">
        <property type="entry name" value="Cbl_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02761">
        <property type="entry name" value="Cbl_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02762">
        <property type="entry name" value="Cbl_N3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47668">
        <property type="entry name" value="N-terminal domain of cbl (N-cbl)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51506">
        <property type="entry name" value="CBL_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="17 24" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="21" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="11 24" id="KW-0808">Transferase</keyword>
    <keyword evidence="18" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="15 24" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="16 24" id="KW-0862">Zinc</keyword>
    <keyword evidence="14 23 24" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Cbl-PTB" evidence="28">
        <location>
            <begin position="31"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="27">
        <location>
            <begin position="365"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="26">
        <location>
            <begin position="852"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="416"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="748"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="25">
        <location>
            <begin position="470"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="25">
        <location>
            <begin position="520"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="589"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00067639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU367001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="903" mass="100242" checksum="5B2FF67984F27B43" modified="2018-12-05" version="1">MAGNLKKGGGIIGMMKDAFQPHHHHLHSHHQPGAVDKKTVEKCWKLMDKVVRLCQNPKLALKNSPPYILDLLPDTYQHLRTILSRYEGKMETLGDNEFFRVFMENLTKKTKQTISLFKEGKERMYEENSQPRRNLTKLSLIFSHMLAELKAIFPNGLFQGDNFRITKADAAEFWRRAFGDKTIVPWKVFRQSLHEFHNISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWSSLLRNWNSLAVTHPGYMAFLTYDEVKARLQKFIHKPGSYIFRLSCTRLGQWAIGYVTADGNILQTIPHNKPLFQALIDGFREGFYLFPDGRTQNPDLTGLCEPSPQDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTAWQESEGQGTGCPFCRCEIKGTEPIVVDPFDPKDNSGGSSRAAMGAEGAPSPSYDDDDDDRLEDPHLVMSKLAGTKVDRPPSPMSMPPQSSLPPVPPRLDLLPHRPPNPPGASSPGATSKAASHHKDKPLPLPPALRDLPPPPPPDRPHPGGAAPEARHPRRPLPCTPGEPLRDKLPPTPPNRPLADWNSRPVPKAPTPSSSSSSPSSSSTHLSNRHSLPLALPSALDNRVDSQKNNSSLSLDHQLSLAAIVSQDYDNPKVKPSSSANAIYALANRPSQGLRQVPGEEACDSEEDSEYMIPSSRPIAAPSVPDTLQVQRPPQPQSLSHGLSFKPIICCCSLTLGAEDGPISHEAMYSVQPRSQQARDSDYSELPPLPVTNGPRDHAAEDREEEDNGYDFPKPRLPQPPARRTFSEMGGSLSTSSSSSSRLSLDSDFGATAYCVKGHVSFLRLNYNYKPSFNPLISSEIEHLMSQGYSYQDIQKALMIAQNNIEMAKNILREFVSVPSTAHIFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MC04</accession>
    <name>A0A3B5MC04_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dihydropyrimidinase-related protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">DPYSL2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MC04"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021070"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021329.1">
        <property type="protein sequence ID" value="ENSXCOP00000021070.1"/>
        <property type="gene ID" value="ENSXCOG00000015763.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234527"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016812">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01314">
        <property type="entry name" value="D-HYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.40.10:FF:000021">
        <property type="entry name" value="Dihydropyrimidinase-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000174">
        <property type="entry name" value="Dihydropyrimidinase-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011778">
        <property type="entry name" value="Hydantoinase/dihydroPyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050378">
        <property type="entry name" value="Metallo-dep_Hydrolases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02033">
        <property type="entry name" value="D-hydantoinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647:SF56">
        <property type="entry name" value="DIHYDROPYRIMIDINASE-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11647">
        <property type="entry name" value="HYDRANTOINASE/DIHYDROPYRIMIDINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="10">
        <location>
            <begin position="65"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="516"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="552"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="516"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="582" mass="63729" checksum="28E0F91EC5D9D29D" modified="2018-12-05" version="1">MSGYQGKKNIPKITSDRLLIKGAKIVNDDQSFLADIYMEDGVIKQIGDNLIVPGGVKIIEAHGRMVMPGGIDVHTRFQMPDRGMVAADDFYQGTRAALAGGTTMIIDHVVPEPGVSLVTAFKQWREWADSKSCCDYSLHVDITEWNNSTQEEMETLVKDHGVNSFLVYLAYKDVFQLSDSQIYEVFSVVRDLGAIAQVHAENGDIVAEEQKRILELGIAGPEGHVLSRPEEVEAEAVNRSVTIANQTNCPLYITKVMSKSAADVIALARKKGAVVYGEPISASLGTDGTHYWSKNWAKAAAFVTSPPLSPDPTTPDYLNSLLSCGDLQVTGSAHCTFHTSQRAVGKDNFTMIPEGTNGTEERMSLVWDKCVVTGKMDENQFVAVTSTNAAKIFNLYPRKGRIDVGSDADLVLWDPDLTKIISAKSHNLAVEYNIFEGTEVRGGPLVVISQGKIVLEEGTLHTTEGCGRYVCRKPFSDHVYKRIKARSRLTELRGVPRGQYDGPVCEVTVTPKVMSTVSSMKTSPAKQQQQQQQQQYTHQPVRNLHQSGFSLSGAQIDDNVPRRTTQRIVAPPGGKANITSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCN5</accession>
    <name>A0A3B5MCN5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">RAB22A, member RAS oncogene family</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Phagosome membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027858875.1">
        <property type="nucleotide sequence ID" value="XM_028003074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCN5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022013"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022280.1">
        <property type="protein sequence ID" value="ENSXCOP00000022013.1"/>
        <property type="gene ID" value="ENSXCOG00000016452.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114135632"/>
    <dbReference type="KEGG" id="xco:114135632"/>
    <dbReference type="CTD" id="57403"/>
    <dbReference type="GeneTree" id="ENSGT00940000157009"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000346">
        <property type="entry name" value="RAB31, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="194" mass="21569" checksum="1084E13237A05E96" modified="2018-12-05" version="1">MALRELKVCLLGDTGVGKSSIVCRFVEDSFDPNINPTIGASFMTKTVQYQNEMHKFLIWDTAGQERFRALAPMYYRGSAAAIIVYDITKEDSFQTLKNWVKELRQHGPPNIVVAIAGNKCDLLDSREVPEKDAKDYADSIHAIFVETSAKNAININEVFVEISKRIPVADSAAATSGKSFKLGRQVSESRRTCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MD70</accession>
    <name>A0A3B5MD70_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibroblast growth factor</fullName>
            <shortName evidence="2">FGF</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">FGF13</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027865194.1">
        <property type="nucleotide sequence ID" value="XM_028009393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MD70"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021470"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021732.1">
        <property type="protein sequence ID" value="ENSXCOP00000021470.1"/>
        <property type="gene ID" value="ENSXCOG00000016072.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114139439"/>
    <dbReference type="CTD" id="492758"/>
    <dbReference type="GeneTree" id="ENSGT00940000162313"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23329">
        <property type="entry name" value="beta-trefoil_FGF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000001">
        <property type="entry name" value="Fibroblast growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00263">
        <property type="entry name" value="HBGFFGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="214"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="216"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="246" mass="27658" checksum="57AE4ED5478C647E" modified="2018-12-05" version="1">MAAAIASSLIRQKRQAREREKSNACRCVSSPSKAKGSCEKPNRLNVFSRVKLFGSKKRRRRRPEPQLKGIVTKLYSRQGFHLQLQADGTIDGTKEEDSSYTMFNLIPVGLRVVAIQGVQTKLYLAMNSEGYLYTSEHFTAECKFKESVFENYYVTYSSMIYRQQQSGRGWYLGLNKEGEIMKGNHVKKNKPAAHFLPKPLKVAMYREPSLHDLTEFSRSGSGTPTKSRSASAVLNGGKAVSQNEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5ME63</accession>
    <name>A0A3B5ME63_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Vacuolar proton pump subunit B</fullName>
            <shortName evidence="15">V-ATPase subunit B</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Vacuolar proton pump subunit B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In renal intercalated cells, can partially compensate the lack of ATP6V1B1 and mediate secretion of protons (H+) into the urine under base-line conditions but not in conditions of acid load.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex. The V1 complex consists of three catalytic AB heterodimers that form a heterohexamer, three peripheral stalks each consisting of EG heterodimers, one central rotor including subunits D and F, and the regulatory subunits C and H. The proton translocation complex V0 consists of the proton transport subunit a, a ring of proteolipid subunits c9c'', rotary subunit d, subunits e and f, and the accessory subunits ATP6AP1/Ac45 and ATP6AP2/PRR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasmic vesicle</location>
            <location evidence="11">Clathrin-coated vesicle membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">Secretory vesicle</location>
            <location evidence="12">Synaptic vesicle membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Melanosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5ME63"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021835"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022099.1">
        <property type="protein sequence ID" value="ENSXCOP00000021835.1"/>
        <property type="gene ID" value="ENSXCOG00000016309.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155068"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18112">
        <property type="entry name" value="ATP-synt_V_A-type_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18118">
        <property type="entry name" value="ATP-synt_V_A-type_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01135">
        <property type="entry name" value="V_A-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12240:FF:000001">
        <property type="entry name" value="V-type proton ATPase subunit B, brain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005723">
        <property type="entry name" value="ATPase_V1-cplx_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003235">
        <property type="entry name" value="PRK04196.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01040">
        <property type="entry name" value="V-ATPase_V1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389:SF5">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B, BRAIN ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039114">
        <property type="entry name" value="V-ATPsynth_beta/V-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 15" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="9 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="17">
        <location>
            <begin position="9"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="16">
        <location>
            <begin position="132"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="18">
        <location>
            <begin position="364"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="469" mass="52076" checksum="4E0179C5195AC75B" modified="2018-12-05" version="1">LCISAYKTVSGVNGPLVILDKVKFPKYAEIVHLTLPDGTKRSGQVLEVTGSKAVVQVFEGTAGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPIDRGPAVLAEDFLDIMGQPINPQCRIYPEEIIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVMDYSEDNFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTSAEYLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHSDVSNQLYACYAIGKDVQAMKAVVGEEALTADDLLYLEFLTKFEKNFISQGAYENRSVFETLDIGWQLMRIFPKEMLKRIPQNTLAEFYPRESKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MER6</accession>
    <name>A0A3B5MER6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MER6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018134"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018365.1">
        <property type="protein sequence ID" value="ENSXCOP00000018134.1"/>
        <property type="gene ID" value="ENSXCOG00000013669.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177384"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="129" mass="14604" checksum="2FA20C9FA73E63A2" modified="2018-12-05" version="1">MCGKLKLSTWKVQLAVLQAMKAYFQGLLLLEKGNEDMNALSQILTEACTALTYSLENKSYSSVRTEALSVVDLIVKRTGESEQWDCMPVRSREQLQRSLSTLQSDSRPELRDKAQELWVELECECSHSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGC8</accession>
    <name>A0A3B5MGC8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGC8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020166"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020413.1">
        <property type="protein sequence ID" value="ENSXCOP00000020166.1"/>
        <property type="gene ID" value="ENSXCOG00000015113.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155063"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032433">
        <property type="term" value="C:filopodium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032426">
        <property type="term" value="C:stereocilium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051491">
        <property type="term" value="P:positive regulation of filopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030832">
        <property type="term" value="P:regulation of actin filament length"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052409">
        <property type="entry name" value="Myosin-III_kinase_activity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46256">
        <property type="entry name" value="AGAP011099-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46256:SF4">
        <property type="entry name" value="MYOSIN-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="22"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="158" mass="18215" checksum="328AFBFAEBA35B51" modified="2018-12-05" version="1">HTHTHTQSSLRDYFLAACLCSGWRCPTIGKGTYGKVYKVLNKKDGSKAAVKILDPIHDIDEEIEAEYNILKALSDHPNVVKFYGMFYKKDLKCGDQLWLVLELCNGGSVTDLAKGMLKRGERMEEAIIAYILHEALKVRVVFFFCRFLLWFLLQRLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGD8</accession>
    <name>A0A3B5MGD8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Potassium channel tetramerization domain containing 10</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BACURD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGD8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022802"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023078.1">
        <property type="protein sequence ID" value="ENSXCOP00000022802.1"/>
        <property type="gene ID" value="ENSXCOG00000017039.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036302">
        <property type="term" value="P:atrioventricular canal development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003157">
        <property type="term" value="P:endocardium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001947">
        <property type="term" value="P:heart looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035024">
        <property type="term" value="P:negative regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033339">
        <property type="term" value="P:pectoral fin development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900125">
        <property type="term" value="P:regulation of hyaluronan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002040">
        <property type="term" value="P:sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd18399">
        <property type="entry name" value="BTB_POZ_KCTD10_BACURD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000013">
        <property type="entry name" value="BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045068">
        <property type="entry name" value="BACURD1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11145:SF14">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING ADAPTER FOR CUL3-MEDIATED RHOA DEGRADATION PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="7">
        <location>
            <begin position="29"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="266"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="298"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="308" mass="34644" checksum="DEB7BBC7D7A418A7" modified="2018-12-05" version="1">MSGDSVVSSAVPAATTRTTSFKGSSPSSKYVKLNVGGALYYTTMQTLTKQDTMLKAMFSGRMEVLTDSEGWILIDRCGKHFGTILNYLRDGAVPLPESRRETEELLAEAKYYLVQGLADECSAALQKKETYEPLCKVPLMTSCKEEQRLIATSNKPTVKLLYNRSNNKYSYTSNSDDNMLKNIELFDKLSLRFNGRVLFIKDVIGDEICCWSFYGQGRKIAEVCCTSIVYATEKKQTKVEFPEARIYEETLNILLYESHDGRGPDNALLEATGGAAGRPSHLDDDEEPIGRVRRIHVKRPDDRTHHHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGK0</accession>
    <name>A0A3B5MGK0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Complement C1q subcomponent subunit A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">C1QA</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">Core component of the complement C1 complex, a calcium-dependent complex composed of 1 molecule of the C1Q subcomplex, 2 molecules of C1R and 2 molecules of C1S. The C1Q subcomplex is composed 18 subunits: 3 chains of C1QA, C1QB, and C1QC trimerize to form 6 collagen-like triple helices connected to six globular ligand-recognition modules (C1q domain). Interacts with CR1 (via Sushi 24 and Sushi 25 domains). Interacts (via C-terminus) with CD33; this interaction activates CD33 inhibitory motifs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGK0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023724.1">
        <property type="protein sequence ID" value="ENSXCOP00000023443.1"/>
        <property type="gene ID" value="ENSXCOG00000017501.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006958">
        <property type="term" value="P:complement activation, classical pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF26">
        <property type="entry name" value="COMPLEMENT C1Q SUBCOMPONENT SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0180">Complement pathway</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="9" id="KW-0391">Immunity</keyword>
    <keyword evidence="6" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017414524" description="Complement C1q subcomponent subunit A" evidence="16">
        <location>
            <begin position="23"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="17">
        <location>
            <begin position="119"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="66"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00093497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="261" mass="27676" checksum="D29FB8EF46F302D6" modified="2018-12-05" version="1">MGADLVCAVLLAVALLPSAVEGDVNCNGRPGELGPPGRDGLAGMKGQKGEPAVRVDGPVDTSMLLRLRGDKGSSGPQGPMGPKGYRGDVGAAGMAGKPGPPGPPGSTAPSALGGQSSSNQETHSAFSVRRNVTTYPPFNQKITYQEAVVNTNNNFAINTGIFTCRTPGFYYFTFHSIAKVPPLCYTAILAKNKIVFCDENTRNSEQVLSGGVVLQLTAGQQVWLESYRDQQQNLQIQKDVDAKDVREKLIIFNGFLIFSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGX4</accession>
    <name>A0A3B5MGX4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Neuregulin 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the neuregulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGX4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022997"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023274.1">
        <property type="protein sequence ID" value="ENSXCOP00000022997.1"/>
        <property type="gene ID" value="ENSXCOG00000017149.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158778"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055013">
        <property type="term" value="P:cardiac muscle cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060347">
        <property type="term" value="P:heart trabecula formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007422">
        <property type="term" value="P:peripheral nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098773">
        <property type="term" value="P:skin epidermis development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003222">
        <property type="term" value="P:ventricular trabecula myocardium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000107">
        <property type="entry name" value="Myosin, light chain kinase a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040180">
        <property type="entry name" value="Neuregulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002154">
        <property type="entry name" value="Neuregulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057909">
        <property type="entry name" value="NRG2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11100">
        <property type="entry name" value="HEREGULIN-NEUREGULIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11100:SF24">
        <property type="entry name" value="PRO-NEUREGULIN-2, MEMBRANE-BOUND ISOFORM ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02158">
        <property type="entry name" value="Neuregulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25518">
        <property type="entry name" value="NRG2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0339">Growth factor</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="7 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017440233" evidence="17">
        <location>
            <begin position="20"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="142"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="244"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="344"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="396"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="560"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="400"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="419"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="433"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="584"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="646"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="256"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="275"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="697" mass="77188" checksum="0414895E1416617F" modified="2018-12-05" version="1">MRLLDPVHLSMLLIGVSFACYSPSLNSLQDQAYRSAVVIEGKVQSTPAGNASVEPYRVSVKVLEVWPLNSGGLEREQLVTVGEFGSEAPCAKVKKNHKYIFFMDPTDEPLVFKASFAPVDTSGKNLKKDVGRILCENCAVAPKLKPIKNPIVVGEGSKLMVKCEATGKPVPTYRWFKDGNELTSSRKVKIKSNLKSSKVQINIAKLEDSGNYTCVVENSLGKDNSTGTVNVQSITTTLSPGSGHARRCNDTEKTFCVNGGDCYFIPGINKISCKCPNDFTGDRCQTYVMASFYQHLGIEFMEAEELYQKRVLTITGICVALLVVGILCVVAYCKTKKQRKKMHNHMRQNMCPEHPNRNLANGPNHPGPGPEEIPMVDYISKNVPATEQVIRPATERSFPASHASSRSHNSSTRAHSSTQRHEERTWSLERSESFLSDSPGMMSSSVGTSKCSSPACMEARARRAAHCGYSDPHRPGLQYGDSFDSLGDSPHSDRYVSALTTPARLSPVDFHYSLPPQVPTFQITSPNASHAMSLPPAVHNPYPPEDDQPLLRRYQQRRTYLKNSRGSLPSSPYRLAEEEDYETTQEYLSSREQPKTSGSSETGSRRWKRSRLNGHVAPRGYEASRDYGSRSCLTESESEEDGESTPFLSMQNMNAEPSTIYRPVDTRTSHSSGWHSGRGNMHTRLTQSRSKPDSTPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MH00</accession>
    <name>A0A3B5MH00_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Eukaryotic translation initiation factor 5A</fullName>
            <shortName evidence="9">eIF-5A</shortName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts. Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome: specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome. Acts as ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="9">eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the eIF-5A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MH00"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018859"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019095.1">
        <property type="protein sequence ID" value="ENSXCOP00000018859.1"/>
        <property type="gene ID" value="ENSXCOG00000014195.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003738"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04468">
        <property type="entry name" value="S1_eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000007">
        <property type="entry name" value="Eukaryotic translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000034">
        <property type="entry name" value="Eukaryotic translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048670">
        <property type="entry name" value="IF5A-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019769">
        <property type="entry name" value="Trans_elong_IF5A_hypusine_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00037">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01287">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21485">
        <property type="entry name" value="IF5A-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003025">
        <property type="entry name" value="eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00302">
        <property type="entry name" value="IF5A_HYPUSINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 9" id="KW-0385">Hypusine</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Translation initiation factor 5A C-terminal" evidence="10">
        <location>
            <begin position="82"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="154" mass="16975" checksum="62868E5992AF8B35" modified="2018-12-05" version="1">MADDDFSTADAGASATFPIQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLTGIDIFTAKKYEDICPSTHNMDVPNVIRKDYQVLDIIDGFLTLMEDNGETREDLRLPDGDMGKEIVKKLESGDQFLVTVWRAVADESVVGIKNMTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHH3</accession>
    <name>A0A3B5MHH3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Histone deacetylase complex subunit SAP18</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the tethering of the SIN3 complex to core histone proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Found in a mRNA splicing-dependent exon junction complex (EJC). Component of the heterotrimeric ASAP (apoptosis- and splicing-associated protein) and PSAP complexes consisting of RNPS1, SAP18 and either ACIN1 or PNN, respectively; the ASAP and PSAP complexes probably are formed mutually exclusive. For the ASAP complex, the association of SAP18 seems to require a preformed RNPS1:ACIN1 complex. Forms a complex with SIN3A and HDAC1. Interacts with SUFU.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the SAP18 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027867432.1">
        <property type="nucleotide sequence ID" value="XM_028011631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHH3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023207"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023485.1">
        <property type="protein sequence ID" value="ENSXCOP00000023207.1"/>
        <property type="gene ID" value="ENSXCOG00000017337.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114141162"/>
    <dbReference type="KEGG" id="xco:114141162"/>
    <dbReference type="CTD" id="10284"/>
    <dbReference type="GeneTree" id="ENSGT00390000003152"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.550:FF:000001">
        <property type="entry name" value="Histone deacetylase complex subunit SAP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.550">
        <property type="entry name" value="ASAP complex, SAP18 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017250">
        <property type="entry name" value="Hist_deAcase_cplx_SAP18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010516">
        <property type="entry name" value="SAP18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042534">
        <property type="entry name" value="SAP18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13082:SF0">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT SAP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13082">
        <property type="entry name" value="SAP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06487">
        <property type="entry name" value="SAP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037637">
        <property type="entry name" value="HDAC_SAP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0678">Repressor</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="153" mass="17610" checksum="B65594D7F62310A5" modified="2018-12-05" version="1">MALESRITQEEIKKEPEKPIDREKTCPLLLRVFTTNSGRHHRSDEFARGNVPSSELQIYTWMDATLKELTSLVKEVYPEARKKGTHFSFAIVFPDLRVKMYKLKEIGSTVSGRKGLDDAMTLQSQRFQIGDYLDIAITPPNRAPPLNTRMRPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHZ5</accession>
    <name>A0A3B5MHZ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dipeptidyl-peptidase 6b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHZ5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019189"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019426.1">
        <property type="protein sequence ID" value="ENSXCOP00000019189.1"/>
        <property type="gene ID" value="ENSXCOG00000014425.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154657"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF97">
        <property type="entry name" value="INACTIVE DIPEPTIDYL PEPTIDASE 10-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="3">
        <location>
            <begin position="97"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="2">
        <location>
            <begin position="467"/>
            <end position="665"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="679" mass="75670" checksum="A12DF241BD9B21C0" modified="2018-12-05" version="1">DLGSSDGPPRNWKGIGIAMVVILAVMSLVILSVVILTPESHLLLRSHVTMEDLESEEFKVHDPCAVWLNNEVALRTREGHILTFTLSNNLTTTLLDNSSLAFVFDGDIYYKASVLRDSLRLTSTDEDQKVLNGLSDWTYEEVLLTYAAHWWSMDGARLAYLRINNSVTPVMEIPHFLGGLYPSNMIFPYPKAGSSIPSVSLFVVNLYGPTHTLEMLPPDSLENYISMVSWISSTRLAVRWLNRAQNQSVLCVCEATTGACSEQDLPLFTSDGSVFYTILPAKQGARGEFHHIAGLTVQQPAIPSVPPRFLTSGSWDVTLLCALDEKAGKYFLSTEESRESRHLYVDLNGAFQRHCISCGLIDGCGFFRAVFSPNRTHFVLYCLPGIPKVTIHSLKDPSYVILEDNNLLSAALEDKRLPESLFRTVQADNHLHLKLTLPQGYEANLLPLLIIDGTPGSQTVTEEFSLGWPQVLCSTHGVALAWVDGRSGVGRGQKTVAVDPRKLGSLRVKDYLGVVLLMQLPYVDNRRIALFGKALGGYLALKMLAATDNLFQCAAAVAPITDFRLYAAFSERYLGFPAKEEHAYTTASVLEEVNKLKEENFLIVHGTADRVHFQHSAELLSRLVKVEANYSLQLYPDESHILREPRSVRHFQRTLVNYLQNCLKHSALLDSPEEEEEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MI58</accession>
    <name>A0A3B5MI58_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">S-adenosylmethionine synthase</fullName>
            <ecNumber evidence="11">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="11">Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AdoMet synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MI58"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023315"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023594.1">
        <property type="protein sequence ID" value="ENSXCOP00000023315.1"/>
        <property type="gene ID" value="ENSXCOG00000017410.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183185"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18079">
        <property type="entry name" value="S-AdoMet_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000001">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000003">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="S-adenosylmethionine synthetase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9 11" id="KW-0630">Potassium</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-adenosylmethionine synthetase N-terminal" evidence="13">
        <location>
            <begin position="18"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase central" evidence="14">
        <location>
            <begin position="130"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase C-terminal" evidence="15">
        <location>
            <begin position="252"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="329" mass="36175" checksum="56689818A4C7139F" modified="2018-12-05" version="1">MNGEINGFHNSLIEEDCFLFTSESVGEGHPDKICDQISDAILDAHLKQDPDAKIACETVAKTGMVLLAGEVTSHAVVDYQKVVRETIRQIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHIDRSQEDVGAGDQGLMFGYATDETDECMPLTIILAHKLNAKMAELRRNGTLPWLRPDSKTQVTVQYRQDRGAMLPVRVHTIVVSVQHDEEICLEEMRDALKEKVIKAVVPSVYLDDDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAELCRRVLVQVRCVTKVCCFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MI61</accession>
    <name>A0A3B5MI61_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MI61"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021059.1">
        <property type="protein sequence ID" value="ENSXCOP00000020806.1"/>
        <property type="gene ID" value="ENSXCOG00000015591.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162944"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042102">
        <property type="term" value="P:positive regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000142">
        <property type="entry name" value="V-set domain-containing T-cell activation inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF14">
        <property type="entry name" value="BUTYROPHILIN SUBFAMILY 2 MEMBER A2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="2"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="129" mass="14781" checksum="9A82BDA06C551E19" modified="2018-12-05" version="1">IPTSLNITSQLQVVPTQTDCILPCSFNVGNEVVIHWIRKLGDIQVHSYYYDKDQLGLQNPRYEGRTSLYPEQLGRGNASLRLSNVSVEDEGRYQCYTSTVNGNKETYIQLKVYGTKTFKHWILFTLSCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIY0</accession>
    <name>A0A3B5MIY0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome c oxidase copper chaperone</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Copper metallochaperone essential for the assembly of the mitochondrial respiratory chain complex IV (CIV), also known as cytochrome c oxidase. Binds two copper ions and delivers them to the metallochaperone SCO1 which transports the copper ions to the Cu(A) site on the cytochrome c oxidase subunit II (MT-CO2/COX2).</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with COA1. Interacts with the chaperone CHCHD4; this is important for correct folding and the formation of disulfide bonds that stabilize the structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the COX17 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027878537.1">
        <property type="nucleotide sequence ID" value="XM_028022736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIY0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024288"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024579.1">
        <property type="protein sequence ID" value="ENSXCOP00000024288.1"/>
        <property type="gene ID" value="ENSXCOG00000018135.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114147932"/>
    <dbReference type="KEGG" id="xco:114147932"/>
    <dbReference type="CTD" id="10063"/>
    <dbReference type="GeneTree" id="ENSGT00940000164584"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016531">
        <property type="term" value="F:copper chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071280">
        <property type="term" value="P:cellular response to copper ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1130:FF:000001">
        <property type="entry name" value="cytochrome c oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1130">
        <property type="entry name" value="CytochromE C oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007745">
        <property type="entry name" value="Cyt_c_oxidase_Cu-chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05051">
        <property type="entry name" value="COX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7 14" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR607745-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="68" mass="7454" checksum="3AA2BAD003F538C1" modified="2018-12-05" version="1">MSSLSAASVEIPAVTDGTEEKKKPLKPCCACPETKKVRDACIIEKGEENCRDLIEAHKDCMRSLGFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLF5</accession>
    <name>A0A3B5MLF5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">trypsin</fullName>
            <ecNumber evidence="8">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLF5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020334"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020582.1">
        <property type="protein sequence ID" value="ENSXCOP00000020334.1"/>
        <property type="gene ID" value="ENSXCOG00000014503.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244971"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000077">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF58">
        <property type="entry name" value="SI:DKEY-33M11.8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="10">
        <location>
            <begin position="40"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="265" mass="29694" checksum="D90204FD3AEEBA3E" modified="2018-12-05" version="1">DQAGSRQHVWSLPSLKMEQVSSHLRVIALRAAFASLDSRIIGGQEVQPYSIKYQASLQTEKGVHYCGGTLVDKEWVVSAAHCWRPSHLMIVVLSEHSLRETEGFEQIFGVSKIHVHNYNYKTFNNDIMIIKLDRPAVLNAYVQPVRLPGENIPQLKGDFCTVSGWGVTQPYSYVLSPVLRAVDVKEITMCSWYYWGRITENMICAGSPYGGKDSCQGDSGGPLVCNGNFEGIVSWGISCANPYYPGVYTKVRNYVPWINYIMNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MML6</accession>
    <name>A0A3B5MML6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Mitogen-activated protein kinase kinase kinase 20a</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MML6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025664"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025973.1">
        <property type="protein sequence ID" value="ENSXCOP00000025664.1"/>
        <property type="gene ID" value="ENSXCOG00000019169.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161352"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000220">
        <property type="entry name" value="mitogen-activated protein kinase kinase kinase 20 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000243">
        <property type="entry name" value="mitogen-activated protein kinase kinase kinase 20 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF288">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="16"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="9">
        <location>
            <begin position="339"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="607"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="678"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="280"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="608"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="624"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="707"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="756"/>
            <end position="773"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="781" mass="88216" checksum="4550155214178551" modified="2018-12-05" version="1">MSSPRASFMHIQFDDVLFYENCGGGSFGSVYRARWISQDKEVAVKKLLKIENEAEILSVLSHRNIIQFYGAIVEAPNYGIVTEYASGGSLYDYLSSDESEEMDMGQIMSWAAEIAKGMHYLHSEAPIKVIHRDLKSRNVVLGADKVLKICDFGASKFLSHTTHMSLVGTFPWMAPEVIQSLPVSETCDTFSYGVVLWEMLTREIPFKGLEGLQVAWLVVEKNERLTIPSCCPESFAELMRKCWTAEPKERPMFKQILCTLESMAKDSQLPEQCNSFLHNKAEWRCEIEATLERLKKLERDLSTKEQELKERERRLKMWERKLIEQSNTPLLPTLDIYTWTEEHVLLIYCLFVSGDGTCDMQLYANLFKENHITGKRLLLLTERDMKDMGVKSKGHVMHLKAEIEKLTNDYLGFLHFPPLLKDELEKEVEQSKTVNLELVFGYHWKAGTGKSDCKWKMYMELDGDEIAVTYIKDVIFNANRPGVEVLRMTKPPFLMDKWIVGLEQNQKIDYTVNYENDVKSPKSTRHSCVLVWTHSGGQDEIKNVELVIETTPVMADTRDRTNCSKLLLLKDIFKEILFSEAQTLPQFLSAHESQAFSYAAALRRSPNHLRSSPWTNSRCSSPTGSLSAKLSPLSLGSKGSSPSSTTSESTLERERPRSAGGMYDQRRNSYFGKALAVGGGQGRGHWWTNNRGSNKGGKGSRQPGKARSNTYSVTPQSRPPLIPGIWSVTGNPSEEKEGGKAGDGGWIKVERHKKPPRQDNKPARGRGRREGRGGHGAGGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MMR0</accession>
    <name>A0A3B5MMR0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein kinase (cAMP-dependent, catalytic) inhibitor gamma</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PKI family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MMR0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020754"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021007.1">
        <property type="protein sequence ID" value="ENSXCOP00000020754.1"/>
        <property type="gene ID" value="ENSXCOG00000015560.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002707"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004171">
        <property type="entry name" value="cAMP_dep_PKI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15416">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE INHIBITOR/PKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02827">
        <property type="entry name" value="PKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="75" mass="8068" checksum="1BD8B997F55537F0" modified="2018-12-05" version="1">MMDVETSYSDFINCDRTGRRNAVPDITGEGAATASTSELTKDMAEMDLKAAGGPTLVREGCLGKQTRVRLTLCHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MN14</accession>
    <name>A0A3B5MN14_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Immunoglobulin subtype domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Immunoglobulin"/>
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            <dbReference type="Ensembl" id="ENSXCOP00000020854.1"/>
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    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="15698" checksum="F9D13A3FE8866B65" modified="2018-12-05" version="1">MVFLAALCGAKTLIQTEGGSITAKCSFTFSGSGKCVCRDPCENGDVLIETEAAEAQKGRYSIRYTEGTFPASSTVLYVNISRLIQSDSGRYWCGLKRRFMTDSRQEVFNFMIFNFTTRIFNDYFHLILITKLLIYASVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNG1</accession>
    <name>A0A3B5MNG1_9TELE</name>
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        <submittedName>
            <fullName evidence="3">Cms1 ribosomal small subunit homolog</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="32473.ENSXCOP00000025076"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025381.1">
        <property type="protein sequence ID" value="ENSXCOP00000025076.1"/>
        <property type="gene ID" value="ENSXCOG00000018724.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR032704">
        <property type="entry name" value="Cms1"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017392795" evidence="2">
        <location>
            <begin position="23"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="292" mass="33784" checksum="F11C44B4BCDABCD4" modified="2018-12-05" version="1">MKNFFQLLLYFFFFFFTDAVEADEESEQEKEPAEKAKTKPEKRKMVTEKSPKAKKKKNNDQVVPFVCQYHPFCFFIIGLNMFRFQRKKKTITDVLATSEPKPGDAADLQDLITRYYSDKRSVIELEELKLQNSCFLSSNDLTHTLSSYLKQVCPKWAKIQKQHSEKSSVVILIVCSSALRTIELLKQLTAFKGEARAVKLFAKHIKIEEQVKLLQKGITHIGVGTPGRISALIDKEGLSLQALRYLVVDWNWRDQKLRRMVDIPEIKLDFLKLLENEVLAACKEDKVKIGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQM9</accession>
    <name>A0A3B5MQM9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Urotensin-2 receptor 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQM9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026083"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026397.1">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:urotensin II receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:skeletal system morphogenesis"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="UROTENSIN-2 RECEPTOR 3"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
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    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="31"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="51"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="347" mass="37473" checksum="7F84076D012873FE" modified="2018-12-05" version="1">MYVISHVKGAAGSANLSAPSSSPSISPSPSFASTALFCSLLSLLSLLGITGNLYTLILLLRRSRVRRRRGAGLACCLTRIPVLSLALADLLYLFTAPFIVYDSLASGWAFGELGCRLLLSLDLLTMHASIFTLTAMSMDRYRAVAHPLRTSSSSSSNLLRVTLAWGLAVALSLPMMITLHLEDGEGGEGQLCVPAWDEQSSKAYLSVLFCTSILGPGLAIGGLYATLGRLYWVSQTQPAWANGTGAASTPRAPKPKVLLLILGIVLAFWACFLPFWIWQLLPLYQPDMLRTLPVGTQVTVNRILTGLTYGNSCVNPFFYTLLTGKRKPNRQMLASANQLCRKGSPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQN3</accession>
    <name>A0A3B5MQN3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">KH homology domain-containing protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Brings lots of money 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 14">Pre-mRNA Splicing factor protein khdc4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">RNA-binding protein involved in pre-mRNA splicing. Interacts with the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome. Involved in regulating splice site selection. Binds preferentially RNA with A/C rich sequences and poly-C stretches.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the KHDC4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQN3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026088"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026402.1">
        <property type="protein sequence ID" value="ENSXCOP00000026088.1"/>
        <property type="gene ID" value="ENSXCOG00000019487.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047412"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22385">
        <property type="entry name" value="KH-I_KHDC4_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22386">
        <property type="entry name" value="KH-I_KHDC4_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000066">
        <property type="entry name" value="KH domain containing 4, pre-mRNA splicing factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000035">
        <property type="entry name" value="KH domain-containing 4, pre-mRNA-splicing factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047890">
        <property type="entry name" value="KHDC4_KH-I_first"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047889">
        <property type="entry name" value="KHDC4_KH-I_second"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056149">
        <property type="entry name" value="PRP5/DDX46/KHDC4_KH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031121">
        <property type="entry name" value="RIK/BLOM7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15744">
        <property type="entry name" value="BLOM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15744:SF1">
        <property type="entry name" value="KH HOMOLOGY DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23469">
        <property type="entry name" value="KH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="ATP-dependent RNA helicase PRP5/DDX46/KHDC4 KH" evidence="17">
        <location>
            <begin position="74"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="KHDC4/BBP-like KH-domain type I" evidence="16">
        <location>
            <begin position="219"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="325"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="445"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="514"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="454"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00078995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF22675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF23469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="567" mass="60404" checksum="264D8E620E1C8E19" modified="2018-12-05" version="1">DEKRVRSSGGSRSSGGMREAEAAPCGALDAAAAVAAKINAMLVAKGKLKPSQISSPLSSDKPPAPTKAKDDLVVAEVEINDVPLSCRNLLTRGQTQEEISKVSGAAVSTRGRYMTADEKNKALLGDRPLYLHVQGQTRELVDRAVNRIKEIITNGVVKAATASSAAPSFSPSGASVTIYQQHNPPAPSLPPATNHKPHFQSGMHYVQDKIFVGLEHAVPGFVVKERVEGPACSYLQHIQAETGAKVFLRGKGSGCLEPASGREAFEPMYIYISHPKPEGLAAAKTLCENLLLTVMHHVKAVDSVCSSPLPPLGFSQPPVVNGIPPQPPYYPPAGFQPSYSVPGPPPQPQPVPPTYTVPPPAPPIMQSSSHGVASQYPIRPSSAPLPAPIPTLPPLHFAPPAAPPKLPLQAAPINPPQKRRFTEEVPDERDSGLLGYQHGPIHMTNLGAGFSVGSAETSGPTQQSSSGPASERDSRHMMPPPTSLRVNGVRHQMEDRRLHQAFVEPPVKRLRSGLVAYSGDSSDEEEDHPSSKASWLGNHGGYRGPPPLPTRAKTQPQQRSIPFWMAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MR06</accession>
    <name>A0A3B5MR06_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Reactive intermediate imine deaminase A homolog</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MR06"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026849"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027178.1">
        <property type="protein sequence ID" value="ENSXCOP00000026849.1"/>
        <property type="gene ID" value="ENSXCOG00000020050.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00420000029792"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00448">
        <property type="entry name" value="YjgF_YER057c_UK114_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.40:FF:000001">
        <property type="entry name" value="L-PSP family endoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006056">
        <property type="entry name" value="RidA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00004">
        <property type="entry name" value="Rid family detoxifying hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="2-IMINOBUTANOATE/2-IMINOPROPANOATE DEAMINASE RIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803:SF39">
        <property type="entry name" value="2-IMINOBUTANOATE_2-IMINOPROPANOATE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="131" mass="14009" checksum="FFB2B1ED35E19559" modified="2018-12-05" version="1">MSALSRKLVHTTSAPAAIGPYSQAVVADRTMYISGQIGMDVASGQLVDGGVQAQTKKALSNMGEILKAAGCDYSNVVKTTVLLTDINDFNSVNDVYKTFFSTNFPARAAYQVAALPRVSTDPNLQIHLLHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRS0</accession>
    <name>A0A3B5MRS0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="RefSeq" id="XP_027891993.1">
        <property type="nucleotide sequence ID" value="XM_028036192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRS0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026201"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026519.1">
        <property type="protein sequence ID" value="ENSXCOP00000026201.1"/>
        <property type="gene ID" value="ENSXCOG00000019575.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114156023"/>
    <dbReference type="KEGG" id="xco:114156023"/>
    <dbReference type="CTD" id="777709"/>
    <dbReference type="GeneTree" id="ENSGT00940000155721"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="127"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="298" mass="33669" checksum="0A1706249F83A8E2" modified="2018-12-05" version="1">MKSPAHRSRDIERQAGYLKPEHCAPPPPRSSSGTMWFIHDSCGIVCGIITWLLVFYAEFVVVFVLLLPAKSVAYSFFNGLLFNGLAFLALASHARAMCTDPGAVPKGNATKEFIESLQLKPGQVVYKCPKCCSIKPDRAHHCSVCKRCIKKMDHHCPWVNNCVGENNQKYFVLFTMYIALISLHALIMAAFHFVFCFDEDWAKCSNFSPPATVILLILLCFEGLLFLIFTAVMFGTQVHSICSDETGIEQLKKEERRWAKRSKWMNMKVVFGHPFSIAWLSPFATPDHGKADVYQYIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MS28</accession>
    <name>A0A3B5MS28_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Multidrug and toxin extrusion protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MS28"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023991"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024276.1">
        <property type="protein sequence ID" value="ENSXCOP00000023991.1"/>
        <property type="gene ID" value="ENSXCOG00000017915.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163922"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="408"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="441"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="579"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="633" mass="69295" checksum="2F9F858FA6034F54" modified="2018-12-05" version="1">MERPGPLEPAPPLQGAEPVAGVPAAKMEAPPVDEGATFASSKLFWCACVKRWLPLAYREELYHVLRLTGPLLLSRILNFLLPFVITIFCGHIGNAELAGYALASATINVTTTATGYGLTMACDTLISQTYGSRNMKRVGVILQKSTLILLMFCLPCWALLVNSHNLLLLLQQEHEVARYILHKKNNFCLFLFQAMFLHQLQVSYLQNQGIILPQMYTAAVANILNLGINYLLISELKLGVTGSAAANSLSQIIICLLLFGYIRWKKLYKQTWGGWSTECLQGWGSYMKLAVPSAFMICFEWWVWEVGGFLAGWLGEDDLAAQHLMVEIGAITYMFPLGIHAAACVRVGNALGAGNTSRAIITCKVALVLSGVLALFQGFILAGCKSVVGYIFTSDEKIVQLVSDNLTVYIFLQFLDALLCVCSGILVGCGMQKIAALSNLIFYYLIGLPVGIALMFWAKLRILGLWLGLLICVFIQTGFFLALIFKADWKKVTQKAQRRAGKTVVVAPVHPVSAVLNEAILPDVIVCPDSAQEKSETALRTEGYSRVNTQDQELKGGRESEESHTNKLSTSQLIMRRGLTLLFSVLILITGVVFQIAFPVAAPSAQSVANATLDWVNFPTPTPLDALNLTTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSP5</accession>
    <name>A0A3B5MSP5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="4">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSP5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024517.1">
        <property type="protein sequence ID" value="ENSXCOP00000024226.1"/>
        <property type="gene ID" value="ENSXCOG00000018093.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158407"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032228">
        <property type="term" value="P:regulation of synaptic transmission, GABAergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16222">
        <property type="entry name" value="EFh_PRIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13364">
        <property type="entry name" value="PH_PLC_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000005">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000025">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000157">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000001">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015359">
        <property type="entry name" value="PLC_EF-hand-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF102">
        <property type="entry name" value="INACTIVE PHOSPHOLIPASE C-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09279">
        <property type="entry name" value="EF-hand_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16457">
        <property type="entry name" value="PH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="PI-PLC Y-box" evidence="8">
        <location>
            <begin position="542"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="7">
        <location>
            <begin position="630"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="506"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="995"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="515"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="996"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1020"/>
            <end position="1030"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1030" mass="113340" checksum="22705E8798FB918A" modified="2018-12-05" version="1">SAGVTVSAGDRSLSQRADRFTLLLPGVLVSLQDPSVQKVGGGRKKTVSFSSMPSEKKVSSAADCLAFMQGGCELKKIRPNSRMYCRFYTLDTDLSCLRWEPSKKDSERARLDVSTIREVRTGKSTETFLHNGPLSEHLAEEAAFSIIHGDEYQSLDLVALSADVANIWVTGLRYLLAHPSIIEGSLGSKMRSEWLAAEFALVDEDGYGIVSEDVAVTTVCKLCPGIKEAKVRLRFKEIQRSKEKLTSHVTREEFQEAFCELCTRPDVYFLLVQLSQDRECLDPQDLRLFLETEQGLSLATTEGCWELLKRYEPSAQGRERGLLGLDGFARYLQSPECQLLDPEHLGVCQDMNLPLSHYYISTSYRSYLLDDQVHGRADLGGLIKALQSGCRCLELGVTDGPEGEPLLGVDYGPDVSRHHHHLHHHHAPVTIRSALEVVNKYAFLTSQYPLLLYLCHRCSPGQQRTMAHHLKKVFGSRLYTPDAQHVSLGGRATTLPSPEQLKGRILLVGKKLPPEEEGSDGEVSEEDEEIGGGGPLQQSPPSSPSSPEAGRLTSESPEDLVVFTKRTLTRVRPSSVRLDSSNPNPQGYWKGGVQLVALNQQTPGAMLDLHRGRFTQNGGSGYVLRPAVMRDEVSYFSAHTHGCVPGVPPQTLRIKVISAHNLPKPQGSGAKGEVIDPYVVLELHGVPADCAEQRTRTAAQNQDEPMFDETFEFQVNMPELALLRFVVLDDDYIGDDFIGQYSVAFDCLQPGYRNVPLLGLAGDPLPHTSLFVHVAITNRRGGGKAQRRGLSVRRVGRRGREYVSLRHTGIKALDEVFKQAGGPLKEATDLREDAQCSTAGFKDQCGLPTVAKLKQCIQSLATRLQSPEGAMGATMLLREGYPCLEPQVSLSEATRKLLSAYDTMISAQKQLIENADGVQERIDQVHREGMDLHEDLSRLCEKEGLKGRKMNKAVESFTWNITVLKGQSDLLRGAKMDSLDALRQLALACEACGLTSSSSNSSNSFSTAELHYSSHPASGRRSSTHGNGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSS0</accession>
    <name>A0A3B5MSS0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable serine carboxypeptidase CPVL</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in the digestion of phagocytosed particles in the lysosome, participation in an inflammatory protease cascade, and trimming of peptides for antigen presentation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSS0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026536"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026858.1">
        <property type="protein sequence ID" value="ENSXCOP00000026536.1"/>
        <property type="gene ID" value="ENSXCOG00000019807.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159498"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000096">
        <property type="entry name" value="Carboxypeptidase vitellogenic-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF472">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE CPVL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017303538" description="Probable serine carboxypeptidase CPVL" evidence="9">
        <location>
            <begin position="29"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="482" mass="54531" checksum="ACF70AD50B31508B" modified="2018-12-05" version="1">MKPVPVFRMLRETLALLFLWACLGPAAAARSCSSFFCRKSHRVSGLNGVDPGAPLFITPYLEKGAIDEARKLSMVGKMPGANVKSYSGYFTVNKTFNSNLFFWFFPAQMNKAPVLLWLQGGPGGTSMFGLFVEHGPYVVYKNMTVGLRDYSWTSRYSVLYIDNPVGTGFSFTDDDRGFAQNQDDVGRDLYSALTQFFQLFPEYQSNEFYATGESYAGKYVPAISYYIHKNNPTAKVKINFKGMAIGDGLCDPELMLGGYGEFLYQTGMIDKLQKQYVDKQTDLGVELIQQQKWVEAFKVFDSLLNGDVDPYPSFFQNATGCTNYYNYMTCQEPEDQEYFSQFVTLPDVRKAIHVGNLTFHDGSEVEKHLVQDVMKSIKPWLGILMDNYRVLIYSGQLDVIVAAPLTERFLPTVNWSGAAEYKTAPRFHWKLQPGDTEVAGYVRQVGEFFQVIIRGGGHILPYDQPARSFDMIDRFLSTQGWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSX2</accession>
    <name>A0A3B5MSX2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Myelin protein zero</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the myelin P0 protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSX2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026591"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026915.1">
        <property type="protein sequence ID" value="ENSXCOP00000026591.1"/>
        <property type="gene ID" value="ENSXCOG00000019840.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234556"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043209">
        <property type="term" value="C:myelin sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042552">
        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000193">
        <property type="entry name" value="Myelin protein zero-like 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000920">
        <property type="entry name" value="Myelin_P0-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13869">
        <property type="entry name" value="MYELIN P0 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13869:SF7">
        <property type="entry name" value="MYELIN PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00213">
        <property type="entry name" value="MYELINP0"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="145"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="17"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="219" mass="24466" checksum="DCD2CA4A815B13B0" modified="2018-12-05" version="1">MTTKEYIFASSLCLAAPQASQAITVYTSWERHALVGSDIRLSCTFFSWRSISEDVSFTWRYRPDGSKDSISIAHFAGGEAYTDNKGVFKDRVEFVGNPKRRDGSILIKNLDFSDNGTFTCDAKNPPDIVGRPSSVRLLVFENLPIQAGVITGAIIGVVLGLLVLIVVIYYLMRFLVARRVFSLSVRSVPRLWGLCRGLRCFFGPDKNLKTLVFQGRILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MT77</accession>
    <name>A0A3B5MT77_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carboxylesterase type B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MT77"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027614"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027949.1">
        <property type="protein sequence ID" value="ENSXCOP00000027614.1"/>
        <property type="gene ID" value="ENSXCOG00000020602.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156607"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000379">
        <property type="entry name" value="Neuroligin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051093">
        <property type="entry name" value="Neuroligin/BSAL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43903">
        <property type="entry name" value="NEUROLIGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017202816" description="Carboxylesterase type B domain-containing protein" evidence="3">
        <location>
            <begin position="36"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="4">
        <location>
            <begin position="38"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="302" mass="33147" checksum="D9B2E797025C8083" modified="2018-12-05" version="1">MSVLIGPSWMCCSKAKTSLLLWMGLVSICVAATQAQQHPVATTNYGKLRGVKVTLPNEILGPVEQYLGIPYALAPTGERRFQPPEPPMSWPGIRNATHFAPACPQFLEDRFLLNDMLPVWFTANLDTVVSYVQEQSEDCLYLNIYVPTEDGRHWLKRGKSARGSDLRSSQDIHDENGLKPVMVYIHGGSYLEGTGNMIDGSILASYGNVIVITINYRLGVLGFLSTGDQAAKGNYGLLDQIQALRWIKENIHAFKGDPKRVTIFGSGAGASCVSLLCLSHYSEGMSSSTDTATFTFKTRADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MTM6</accession>
    <name>A0A3B5MTM6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein Jumonji</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Jumonji/ARID domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts as an accessory subunit for the core PRC2 (Polycomb repressive complex 2) complex, which mediates histone H3K27 (H3K27me3) trimethylation on chromatin. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells, thereby playing a key role in stem cell differentiation and normal embryonic development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling. Does not have histone demethylase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MTM6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026856"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027185.1">
        <property type="protein sequence ID" value="ENSXCOP00000026856.1"/>
        <property type="gene ID" value="ENSXCOG00000020057.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159220"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16870">
        <property type="entry name" value="ARID_JARD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000007">
        <property type="entry name" value="Jumonji, AT rich interactive domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.60:FF:000005">
        <property type="entry name" value="Jumonji, AT-rich interactive domain 2a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF113">
        <property type="entry name" value="PROTEIN JUMONJI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="JmjN" evidence="14">
        <location>
            <begin position="414"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="ARID" evidence="13">
        <location>
            <begin position="478"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="15">
        <location>
            <begin position="739"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="155"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="263"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="315"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="367"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="158"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="176"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="276"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="375"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="955" mass="107737" checksum="6B500238E45C730B" modified="2018-12-05" version="1">GKKQRENRNIVIDDSDGIPWSEERVMRKVLYLSLKEFRTAQKRQLDGDGPTNLNGEFRAINKTIMMSQGNTKSDCSSGSCQNPQKLVSSHFNLLFPRLRPRLQAQRKFAQSQPNSPSTTPVKVDLSRRKPKTEDFLTFLCLRGSSALPSNMAYFGSSQEEEDLEEEEDEEEEEVRNTSASSSCNSTPRKGKLPTRQPLNGHGKGQNWRSEQSAPCVSKVNGMTRAGSAQAVGGAKKSHDLRLPSKTVKKYTATVSKGHVTYTKAKQKELGKKTKLSSSNKHNSAASASSNAKTRKQVLLSNGLHKAVVNMRPNGRLNGQWHNASAKEDGQKQCQQGHGECPGREGLRNSKRRLEVVLNSVSTGVVVQKAKTSGSEAKKEWEHEKELTRQKEREQEKERERQRSKPEGWAALGEVPVFRPSQREFQDPLVYLDAVREQAEVAGMCRVVPPQDWRPECKLNEEMRFVTQVQRVHMLGRRWGPNVQRLACIRKHLKSQGITMDEPPVIGGCEVDLSRFFQLINDMGGMQQVMDLKKWTKLADLLRIPKSAQDRLAKLQEAYLQYILSYDSLSAEERLRLLGDVLQEKKNLESRQGPLEGLSDSAAHALPRYEPKNGLVVYHRLKCLKELEAQVKAGRRRLFAQEKQGKEDRHNGEQRMEEDRGILSDQYKCIYKGKSVSLTNFFRIARNTMTMCFNKEPGAAEVEQEYWRIVEQRDAHVAVHCGKVDTSTHGSGFPTGKSEPFSKHGWNLTVLPNNSGSILRHLGAVPGVTIPWLNIGMVFSTSCWSRDQNRLPYIDYLHTGADCIWYSVPAEEKAKLDKVVHTLLQANGTPGLEMLEKNIMISPEVLCREGIKVYRTVQRSGQFVVCFPGAFVSKVCCGYSVSETVHFATPHWMNLGYQAAKDLKFRCIAKPFSMEKLLYQIATAESKRDNGLLLTTISTLLKDLRQDATPHKNTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWF8</accession>
    <name>A0A3B5MWF8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine/threonine-protein kinase receptor</fullName>
            <ecNumber evidence="13">2.7.11.30</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[receptor-protein] + ATP = O-phospho-L-threonyl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44880"/>
            <dbReference type="Rhea" id="RHEA-COMP:11024"/>
            <dbReference type="Rhea" id="RHEA-COMP:11025"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWF8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025752"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026062.1">
        <property type="protein sequence ID" value="ENSXCOP00000025752.1"/>
        <property type="gene ID" value="ENSXCOG00000019249.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156210"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048179">
        <property type="term" value="C:activin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048185">
        <property type="term" value="F:activin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017002">
        <property type="term" value="F:activin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000099">
        <property type="entry name" value="Serine/threonine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000094">
        <property type="entry name" value="Serine/threonine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF70">
        <property type="entry name" value="ACTIVIN RECEPTOR TYPE-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00653">
        <property type="entry name" value="ACTIVIN2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="303" mass="34195" checksum="FCB5C640A283C778" modified="2018-12-05" version="1">MSEYVAVKIFPVQDKQSWQNERDIFLTPGMRHENILRYIAAEKHGTNLETELWLITEFHERGSLTDHLKGNTVTWTELCHIAETMARGLAYLHEDIPSYKGEGPKPTIAHRDFKSKNVLLRDDLTAVIGDFGLAVRFEPGKPPGDTHGQVGTRRYMAPEVLEGAINFQRDSFLRIDMYAMGLVLWELVSRCSETDGTVGEYMLPFEDEIGQHPSLEDLQDVVVHKKMRPAIKDCWLKHPGLSQMCETIEECWDHDAEARLSAGCVEERISQIARTIGSTTSDSPASIVTSLINEDLPPKESST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWL9</accession>
    <name>A0A3B5MWL9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">[histone H3]-trimethyl-L-lysine(27) demethylase</fullName>
            <ecNumber evidence="4">1.14.11.68</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(27)-[histone H3] + 2 2-oxoglutarate + 2 O2 = N(6)-methyl-L-lysyl(27)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:60224"/>
            <dbReference type="Rhea" id="RHEA-COMP:15535"/>
            <dbReference type="Rhea" id="RHEA-COMP:15544"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.68"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UTX family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.68" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWL9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024129"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024417.1">
        <property type="protein sequence ID" value="ENSXCOP00000024129.1"/>
        <property type="gene ID" value="ENSXCOG00000018010.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155202"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044666">
        <property type="term" value="C:MLL3/4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071558">
        <property type="term" value="F:histone H3K27me2/H3K27me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1370:FF:000001">
        <property type="entry name" value="lysine-specific demethylase 6A isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000002">
        <property type="entry name" value="lysine-specific demethylase 6A isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051630">
        <property type="entry name" value="Corepressor-Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048562">
        <property type="entry name" value="KDM6A_B-like_C-hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017:SF9">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21322">
        <property type="entry name" value="KDM6_C-hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13431">
        <property type="entry name" value="TPR_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="90"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="231"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="265"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="9">
        <location>
            <begin position="841"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="401"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="471"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="536"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="661"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="799"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="401"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="424"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="488"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="507"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="570"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="589"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="668"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="799"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1039" mass="113776" checksum="456A80FC5F8A1C36" modified="2018-12-05" version="1">MKSCGLSLTAAARSLGVASNDEEKMASGKATEIEEDFPKLTAQEKESLVGIDSSLFGFHGLQEDGARTKALLLKSDVLSFLFFFLFPQNAAFLYGLGMVYFHYNAFQWAIKAFQEVLYIDPTFSRAKEIHLRLGLMFKVNTDYESSLKHFQLALIDSNLCTLSKAESKSAAKDAYESLLQMENLPAQVKATTLQQLGWMHHTVEQLREKDSKGCYAIQCLQKSLEADPNSGQSWYLLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHSHAAAWMDLGTLYESCGQLQDAMKCYINATRSKACLNTAALAQRIKLLQGAADLLANGSNQQQVPGWYLTPQKIQLLDHLRSNRASLTPPRQHMLEQLENQFSLMQQHQQQGLQKGPGSHPAGPDGEPPFSSSSSPSQTSFSSSSVLNQVGLYAATVAPSGGKTGSTVPAAVPLPGLEAAGGHHGGAPCSPGSASRQGLTNHVQPRVQERSASPLQPASPAAGATLTSDNPQLSAFVKGKANATSNHDDSNHGGSSSEKTNHVLPALHAKPEHSAASSPLSVAPVPSPCSADPHTTSGPSCLNSLSDSNSQGPTLNGKSPEDSRSPQRKTPPAGLAPWSSVSIYPSSSDVLKACRNLGKNGLSTSSILLDRCPPPRPPGRPSPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPANPVTVIRGLAGALKLDLGLFSTKTLVEANPEHLVEVRTQLAQPTDENWDPSGSRKMWRCESSRSHTTILKYAQYQASSFQESLREENEKKKEVEAEAASSDSGIRRRKGPLKHIKFGTNIDLSDDKKWKQQLQELTKLPAFARVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVPESYWGVINDFCEKNNINFLMGSWWPNLEDLYENNVPVYRFIQRPGDLVWLNTGTVHWVQAIGWCNNIAWNVGPLTAHQYKLAVERFEWNKLQSVKSIVPMIHLSWNMARNIKVSDHKLFEMIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXN2</accession>
    <name>A0A3B5MXN2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Calmodulin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXN2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026773.1">
        <property type="protein sequence ID" value="ENSXCOP00000026452.1"/>
        <property type="gene ID" value="ENSXCOG00000019752.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182904"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016286">
        <property type="term" value="F:small conductance calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004178">
        <property type="entry name" value="CaM-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036122">
        <property type="entry name" value="CaM-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015449">
        <property type="entry name" value="K_chnl_Ca-activ_SK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10153">
        <property type="entry name" value="SMALL CONDUCTANCE CALCIUM-ACTIVATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02888">
        <property type="entry name" value="CaMBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03530">
        <property type="entry name" value="SK_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01053">
        <property type="entry name" value="CaMBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81327">
        <property type="entry name" value="Small-conductance potassium channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="304"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Calmodulin-binding" evidence="11">
        <location>
            <begin position="372"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023065"/>
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            <dbReference type="SMART" id="SM01053"/>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="421" mass="48278" checksum="F30AE95E55461095" modified="2018-12-05" version="1">MHDRQSLPEIIITSKDDNLQQQQHKHKALQYHQHPGEAEGPLPGAKRFTRSPSPSPQHSPKRKEDANKDSYSRTHNIGWRLVRRRALFLRRQRLNDCALAVGLFGVVMMVMETEMSWSIYSKVRGLLECKESCNKSHCTAASLICHHINISSFLMLETLYVHDIGAEDWRIAMTTERFALIVLELFIVAIHPYPVGILAYFQKTKVRTTLSETELEIMLALPMFLRLYLLGRAMMLHSRLFTDTASRSIGALNKVGIHFNTRFVGKTLMTTYPGTVLMIFSVSLWIVAAWGLHVCERYVCGEHLMWKPLWMVSVTFLSIGYGDVVPHTYCGRSICLLTGIMGAGCTVLVVAVVARKLELTRAEKHVHNFMMDSHISKRIKIAAANVLRETWLIYKHTKLSRERDHTRVRMHQRKLLLAIHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZ10</accession>
    <name>A0A3B5MZ10_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Si:ch211-154o6.3</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
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        </lineage>
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    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSXCOG00000019192.1"/>
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    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050505">
        <property type="entry name" value="WDR55/POC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019:SF8">
        <property type="entry name" value="POC1 CENTRIOLAR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019">
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
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    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="78"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
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            <begin position="142"/>
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        </location>
    </feature>
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    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="425" mass="46135" checksum="0089022A040574E0" modified="2018-12-05" version="1">QLSGPQQSLGIVGDGNKEKMKRQGEAESKAAGTQGADESTLLIAQIISFFLFFFVFAEAEFSSLTASWEKARFRLRLLVGHNDIVTSVVAVDNLVVSGSRDTTVKVWHVPTATEQKNLGGHTGGVTCLSAPPLECCRRLARSLSLSDKERFILSGSADCNVKIWALSIGQCVKSIYTFNAVTALSFAPEGDGYIITGSDGGKVQAWSWHTFQNCQSVKAHEEAVTSIQSQGPLVFSGSAEGGVSVWENRRSEREPLKLLHHWSSQVTGLSGGPGGRLTLSPRGDRVVLAYGRASIKILHWRTGVISRLTNHSSITGVTDCVHQTGGILIGSCYDLANGESSLNLFSLPQCRYLASLTWPDAPRILCFAAWTTGSGDHRWVTGGRDLIVWEQLPSSGKNRWVRLLPVFQIQSFLFKFVGSYADLAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KL56</accession>
    <name>A0A3B5KL56_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Centrosomal protein 152</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000000843"/>
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        <property type="protein sequence ID" value="ENSXCOP00000000843.1"/>
        <property type="gene ID" value="ENSXCOG00000000701.1"/>
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    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:centriole replication"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CEP152_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057664">
        <property type="entry name" value="CEP152_PLK4_bind"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10337:SF6">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 152 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10337">
        <property type="entry name" value="SHC TRANSFORMING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25770">
        <property type="entry name" value="CC_CEP63-bind_CEP152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25769">
        <property type="entry name" value="PLK4_bind_CEP152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CEP152 CEP63 binding coiled coil" evidence="3">
        <location>
            <begin position="1064"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1184"/>
            <end position="1204"/>
        </location>
    </feature>
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        <location>
            <begin position="1234"/>
            <end position="1366"/>
        </location>
    </feature>
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        <location>
            <begin position="173"/>
            <end position="263"/>
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    </feature>
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        </location>
    </feature>
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            <begin position="490"/>
            <end position="613"/>
        </location>
    </feature>
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        <location>
            <begin position="671"/>
            <end position="702"/>
        </location>
    </feature>
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        <location>
            <begin position="738"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="841"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="988"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1184"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1256"/>
            <end position="1267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF25770"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000843.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1366" mass="157577" checksum="3F256F00530CE75D" modified="2018-12-05" version="1">MSIDFDSAAIQTQHEEDEYDQEDYAREQELHKLLTDLPDDMLEDSRDSSPELESSTYSNKNSGSSPQTKWNQDWSDHSHPTSHTQVVLLCLRIKSTTETINHDESITQIIVKLLNSGPGDHGNNQFKVNYNPQHPAHQPKMLNPHSAQHKDQFEQLQREFLDSTQQTADREQVAQLQVLNKAQQRQIEDLERKLEDSRRNMRYIEHQFAIVKDEKDGLAVSFRESSRLIEEAKKGEAQMQNKLKALEQQVKILKEKDQEVNKLKVADAAVDSMKQQMLELCRSDTLSRARQQHDRDLAVIKEQHEAALLALQQKLDSTSQALNDQIDVSQTLQRQVQQLERQREEEQLERARIVNSLSQRLEESQQQCAKLLQTNSVQEMSQMQIKLQQAQSAKTLSENLNKVLQDMAELKEQITLYESALKHGVIAFDLSNDWENQLSESCMDLGLKKPDRKNGTVHSTTMNHLSDSKLPKEEALRLLRVEMQRCLGCLKGKRQKINQLQEELRLAEGRVGELEAQLEEAQLCSAVSAPPVCFIVNLEMIQELDQEKQEATQRTERINQQYRDDVVNRVRTELLLEHNAEVEQLTAQHQQEIQQLQNQLSEVSDKMLAVQECYISVCKEKCFLEETRQKKQEEKSSEKTWVQKLEEAKKERNAAEVSCQTEIASGRAISAEELDSRLAAQKQQLQLEADKVQRKAVAEARKQIQRELEEKHLNDMAKQVEGAVTRAYSRWIEDLSSLPEYQTLLQRETEKWEELQEEITKEKVSQALQEAEEEWRKNQVEQQKAVDLQEELAALKSQLEQASREQAALLKAELAAARAAWFRDKQQEISIIQARNEQTYQTKLQEQCKNLEQAVQEAREDADLQRKELRTQEEEWRCQYEEKELAERQQKKEKFVLELQTALRQEAEDSRRTSGSPSETTITNIIETSCRDMMNRAVTEAKKEWKKMSEEKLSCILKETQEKHTREINKIQGRKEQSGCRKGCTESLGKLQKKNQELQRHLEKACRQLQHRIREHKTAMQRLRDEHESSIQKLKEEHLQQLEEVKRANESSGSYHQQHLQRGLEEMKQQYLVTVEKIRGDMLRYLQESRERAAEMIRSEVQRERQDTARKMRRYYLTCLQELLEDGGKATGAEKKIMSAASKLAAMAKVLETPVKSNSGNNYSLPSKSSGFRSNLTTLNELLDVRPEQQPQREKVSADSGPKSTAAFRTKHLTHQDSEDGFWFLQRGDSSKAEVGRQSRPLLIQEAPVREEKQTDWSLTSNDSDSGPNAARPRRKAEPLRPFSANSASDADIGALGGAAPDDSDLTVNKEIPKQLPQTENKKAKKSLAREPIPGSDGDVFSRPLFSELRQRQQDSGFDSPFYLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KNH1</accession>
    <name>A0A3B5KNH1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Parvalbumin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6 8">In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the parvalbumin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KNH1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001668"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001693.1">
        <property type="protein sequence ID" value="ENSXCOP00000001668.1"/>
        <property type="gene ID" value="ENSXCOG00000000928.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163861"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16255">
        <property type="entry name" value="EFh_parvalbumin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000060">
        <property type="entry name" value="Parvalbumin, thymic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008080">
        <property type="entry name" value="Parvalbumin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11653:SF4">
        <property type="entry name" value="ONCOMODULIN-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11653">
        <property type="entry name" value="PARVALBUMIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01697">
        <property type="entry name" value="PARVALBUMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="39"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608080-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="139" mass="15251" checksum="5FD0ED27E1DE1E54" modified="2018-12-05" version="1">MSLSSVLSADAIDSAIKDCQAPDSFCPKKFFQLCGLSKKSPQDVKKVFGILDNDASGFIEEEELKFFLQRFRPGARVLTDKETKAFLNAADDDSDGKIGAEGENICLDDMSFQNADKSEPKRKTVNSFLCGVLISLVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KNW2</accession>
    <name>A0A3B5KNW2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nucleoporin NUP188</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Nup188 family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000001823"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001850.1">
        <property type="protein sequence ID" value="ENSXCOP00000001823.1"/>
        <property type="gene ID" value="ENSXCOG00000001468.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005742"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018864">
        <property type="entry name" value="Nucleoporin_Nup188_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044840">
        <property type="entry name" value="Nup188"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048883">
        <property type="entry name" value="Nup188_N-subdom_III"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431">
        <property type="entry name" value="NUCLEOPORIN NUP188 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10487">
        <property type="entry name" value="Nup188_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21093">
        <property type="entry name" value="Nup188_N-subdom_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21094">
        <property type="entry name" value="Nup188_SH3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup188 N-terminal" evidence="11">
        <location>
            <begin position="13"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin Nup188 N-terminal subdomain III" evidence="12">
        <location>
            <begin position="506"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1493"/>
            <end position="1520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1498"/>
            <end position="1508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1642" mass="183521" checksum="6FB9DF3F76389A95" modified="2025-10-08" version="2">MLGRSALREPAQIEGELDRHWDRLHQGLGYYKPPSSSSAGKVKEKKNVAQPLKDFGFRISKLLNLDEQQSVQLLQCYLQEDYRGTRDSLKVVLTDERQSQGLLLKIADYYYEERMCLLRCVLLLLTYFQDERHPYRTEYSNCVNKLEKDLVSSYQSQFENLFKAEAPTWETHGNLMTERQVSRWFMQCLREQSLLLEIIFLYYAYFEMSPSDLLNFTKMFKEQGFGLRQTNRHLVDKNMDALVDRIGYLSSLILVEGMDIDFLHKCALEDCPEQHQFSGAPDMDQLLLTFGDIPHHGPVLLSWVLLRHTLRPDESNPVIRRIGNTALQLGVFQYLSSMLKGLGVSGNNCTASTAKMCIYGLLSFVITSFEEESLQGSHLIDAACEVLSAPSLAEVFWEMPNRGLGMILDSAAGMFPHKIGPLLQLLTALVSNKLTVKKVYNFLDKMSFYTQVYKHKPIDIISKDDETLWRRQAPKLLYPLGSGQANLWMPQGVLGQVMIAGEQGYMVRWDYSYSAWTLFTCEVEMLLHVVSTADVLAHCTRVKPILDLVHKIISTDWTVSDCLLPLTSRIYMLLQRLTSVINPPVDVIASCVNCLSVLAARMPGKVWSSLHHTGFLPFTSNPLTNMAQCISAEGMKAGNYGNLLVQIEQPRGEYAVTIAFLRLITTLVKGQLGNTQSKGLIPCVLLVLKEMLPTYHKWRYNTNGVRERIGCYILELIHAILNLSPEGENQGSTPTLQSLCIYSLANTEAGQAVVNIMAVGVDTIDVVLAAQPSSCGSEGPGQILIQTVKLAFSVTNNVIRLKPPSDAVSPLEQALTQHGGHGSNLVVVLAKYIYHKHDPALPRLAIQLLKRLATVAPMSVYACLGNDAAAIRDAFLARLQSKTEDMRIKVMILEFLTVAVETQPGLIELFLNLEVKDGNEGSKEFLVGEWSCLHVVLELIDSKQQGKYWCPPLLHRAALAFLLALWQDRRDSAISVLRTKERFWENLTTPLFGTLTPPSDTTEPCVLEACAFVMKIIGLEIYYVVSGSLEQSLKDGLQKFSNARRYDYWSQYVKSLVCHVAETEEEGICCFPETQMLISAWRMLLILSTTHADVMHLTEDSTKLKLFMDVLDGTKAALTTPTSVPCLRLGSMMATLLLILLKPWRRCLGSSAPDILSPLSLILESVLQADQQMMERTKAKIFSALISVLQIQGLNGGDISQLPQLLLSVCETVKDEALALIDNTRHMSQTGDLLEQEDSMETDCPRGLQKDQRDGVCVLALHLTKELCRSDEDGEHWVLVMRKVPVLPSVLSAVEISLRCKHNLFFTEAALHLLLTLARTPQGAAAVAGAGVIQTICLPLLSVYEVSSNGTSQSFSRKSQDSACWPGVYRLCMSLMESLLKTLRYNFINEALDFVGVHQERILQCLNAVRTVQSLSCLDEADHTVGFLLQLSSFCKEWQFHLPNLLRDVQATKRCSTITCRLRSESFYYFKSDEEDAVITKCAFSVQSKNGEALPAGTRTQRPPQTLNKDAAAASSSGGGEREEAEQKALLAVQCSLLKILSKTLATLQHFTPDCCQILLDQCMDLAEYRTLFVLSFTTPAFDPDVAPSFGTLLATINVALGTLSEVGCGMPQRSASINQKFKNDDLLLLIFRWRRGRSRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KT99</accession>
    <name>A0A3B5KT99_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="5">AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-AMP-activated protein kinase gamma subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KT99"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000841"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000851.1">
        <property type="protein sequence ID" value="ENSXCOP00000000841.1"/>
        <property type="gene ID" value="ENSXCOG00000000705.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183019"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04618">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04641">
        <property type="entry name" value="CBS_euAMPK_gamma-like_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000004">
        <property type="entry name" value="Protein kinase AMP-activated non-catalytic subunit gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF122">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="14"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="96"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="172"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="243"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="313" mass="35684" checksum="43C8C473EFBDD28D" modified="2018-12-05" version="1">MRFMKCHKCYDIVPTSSKLVVFDTTLQVKKAFFALVANGVRAAPLWESKKQSFVGMLTITDFINILTRYYKSPMVQIYELEEHKIETWRELYLQETFKPLVHISPDASVFEAVHSLIKNKIHRLPVIDPISGNALYILTHKRVLKFLQLFVSLGMPMPAFMKQTLEEHAVGTYSNIAYIHPDTPLITALSVFTHRRVSALPVVDHSEVVRFLTAVFVQNLAAEKTYNNLDVTVTQALRHRSQYFEGVMKCNKLETLETIVDRIVKAEVHRLVVVDDESHIIGIVSLSDILQALVLMPAGTDFIYCLFRASACQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KUS2</accession>
    <name>A0A3B5KUS2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KUS2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001750"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001776.1">
        <property type="protein sequence ID" value="ENSXCOP00000001750.1"/>
        <property type="gene ID" value="ENSXCOG00000001418.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166648"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009954">
        <property type="term" value="P:proximal/distal pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000018">
        <property type="entry name" value="Homeobox A10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017112">
        <property type="entry name" value="HXA9/HXB9/HXC9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45970">
        <property type="entry name" value="AGAP004664-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45970:SF3">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-A9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="20"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="22"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="89" mass="10671" checksum="D1A492700777756D" modified="2018-12-05" version="1">HCSSGGEGEDNPVSNWLHASATRKKRCPYTKHQILELEKEFLFNTYLTRDRRYEVARLLNLTERQVKIWFQNRRMKMKKFNKDGAPKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KVH5</accession>
    <name>A0A3B5KVH5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 2 subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Eukaryotic translation initiation factor 2 subunit beta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027877700.1">
        <property type="nucleotide sequence ID" value="XM_028021899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KVH5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004123"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004168.1">
        <property type="protein sequence ID" value="ENSXCOP00000004123.1"/>
        <property type="gene ID" value="ENSXCOG00000003237.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114147461"/>
    <dbReference type="KEGG" id="xco:114147461"/>
    <dbReference type="CTD" id="8894"/>
    <dbReference type="GeneTree" id="ENSGT00390000001804"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor IF2/IF5" evidence="7">
        <location>
            <begin position="200"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="109"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="132"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="334" mass="38066" checksum="82B2CCB449F8D130" modified="2018-12-05" version="1">MSGDEMIFDPNMTKKKKKKKKPFMLDEEGGEGVGGEEAKEVEVKEAEPEVGEDKEVDIDEDEGRKKELSDDLNDLNFFNQKKKKKKPKKNFGVENDIEEGLKELKIEGEQTEAPEEDNLDLMLPSKKKKSKKVDFDEGEALEKDDALEDDESKNNDGISFISSTGPAWAGTERDYTYDELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPETILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KY61</accession>
    <name>A0A3B5KY61_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Voltage-gated potassium channel subunit beta-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">K(+) channel subunit beta-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Kv-beta-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">KCNAB1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a primary alcohol + NADP(+) = an aldehyde + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15937"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:15939"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a secondary alcohol + NADP(+) = a ketone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19257"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17087"/>
            <dbReference type="ChEBI" id="CHEBI:35681"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:19259"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the shaker potassium channel beta subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KY61"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000489"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000498.1">
        <property type="protein sequence ID" value="ENSXCOP00000000489.1"/>
        <property type="gene ID" value="ENSXCOG00000000421.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156760"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044224">
        <property type="term" value="C:juxtaparanode region of axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005983">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005400">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01293">
        <property type="entry name" value="Kv_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150:SF7">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01578">
        <property type="entry name" value="KCNAB1CHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01577">
        <property type="entry name" value="KCNABCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0630">Potassium</keyword>
    <keyword evidence="8" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="19">
        <location>
            <begin position="79"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="373" mass="41885" checksum="AA1A817EAAD1AEEA" modified="2018-12-05" version="1">MQVSIACTDHNLKRGNGDHSKQSATSPNVVNQARAKFRTVAIIARSFGSFTPRHISLKESTGKHTGMKYRFSVFPSPAGTWVTFGGQITDEVAEQLMTIAYESGVNLFDTAEVYAGGRWVLRSSLVITTKLYWGGNFIFFIYLSGLKGSLQRMQMEYVDVVFANRPDSNTPMEEIVRAMTYVINQGMAMYWGTSRWTAMEIMEAYSVARQFNLIPPVCEQAEYHLFQREKVEVQLPELYHKIGVGAMTWSPLACGIITGKYENGIPESSRASMKSYQWLKEKIVSEDGRKQQAKLKELNHIAEKLGCTLPQLAVAWCLRNEGVSSVLLGTSNPEQLTENLGAIQVLPKMTSHIVSDIDHILGNRPYTKKDYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZA7</accession>
    <name>A0A3B5KZA7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Palmitoyltransferase</fullName>
            <ecNumber evidence="14">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:36684"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZA7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003887"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003930.1">
        <property type="protein sequence ID" value="ENSXCOP00000003887.1"/>
        <property type="gene ID" value="ENSXCOG00000003054.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158044"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12349">
        <property type="entry name" value="ANKYRIN REPEAT AND LEM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12349:SF1">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="173"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="16">
        <location>
            <begin position="91"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="358"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="411"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="587"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="631"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="358"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="377"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="415"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="632"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="682"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="729" mass="80322" checksum="78616D4322837E81" modified="2018-12-05" version="1">MPQVWESQRTTFSFTSTGLFCSPLCPWLAVTICPAVPPCCAVLFLFVLANFTMATFMDAGVLPVAGEDEDKEDEFRAPLYKNVDVKGVQVRMKWCASCHFYRPPRCSHCSVCDHCVEDFDHHCPWVNNCIGRRNYRYFFLFLSSLTVHMIAVFTFGLVYVLHHVDDLWKLHCLITLVVISISGLFLIPVLGLTGFHLYLVSRGRTTNEQVTGKFQGGINPFSRGCCSNMEYLVCSPISPNYTSRTCKKTVILVQPPFLRPEVDRQMPVKIRDNGIQSLAIQNKVCASKCVFQLKNPPPLPPKPDRGLLKSQVAVLDGALELQTHMKKFIMFCWVPDKQCCVFLCFNTQLLKTSEQQGINKGSDLCSNTRGSPARGSHQASVPVSTPLQPSTVSSSLQLNSLTLNSRSLTLKHSSRNGSKSQLLSMNPEGLGSNSSPGIITTPSLLTNQCSSRNSKPSYNNLVRPSDPQFMVQRGAPPVSYHTHFMGLGTDGAVQRFLQRDSSPSIATRDIPPPGLVKRDLTSQGLQDNLRDLYPHDVSLPMSAAARYDNFSKTIMASIQERREQEERERMLRVSARSQVLYDPDVGIYDIPSRRSLPPDNIRPAGSRGPTPPAYGSREFLMSTGILGYGMRTSPLSSSSTSSLTRGPKTSSSPLQSSSSSLQSKGRSTSPAYCPPERHTQGPSSSTSTLPRLSSSAYPTPPYTSYATAKRSSLPFPSEGKESVTLGALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L155</accession>
    <name>A0A3B5L155_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Carbonic anhydrase</fullName>
            <ecNumber evidence="4">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027869623.1">
        <property type="nucleotide sequence ID" value="XM_028013822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L155"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004060"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004104.1">
        <property type="protein sequence ID" value="ENSXCOP00000004060.1"/>
        <property type="gene ID" value="ENSXCOG00000003183.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114142512"/>
    <dbReference type="KEGG" id="xco:114142512"/>
    <dbReference type="CTD" id="766"/>
    <dbReference type="GeneTree" id="ENSGT00940000159757"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF124">
        <property type="entry name" value="CARBONIC ANHYDRASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="5">
        <location>
            <begin position="4"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="262" mass="29748" checksum="F8225A6540F302DB" modified="2018-12-05" version="1">MTGHHWGYGKEDGPSAWHRQFPVAKGNRQSPIDIIPDEARRDSSLGPIFLKYDRCTSLNIANNGHSVVVEFNDSDDRSVIQGGPLDNPYRLKQFHFHWGEKGCEGSEHTVAGAGYASELHLVHWNADKYKTFEEAATAPDGLAVLGIFLKLGDDHRWLHMITDALYMVKFKGSSTDFKGFNPKCLLPSNLHYWTYLGSLTTPPLHESVIWIVLKEPIVVSEKQLGMFRLLQFSGEEDGQRMRMENNFRPPQPLKERLVRSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L180</accession>
    <name>A0A3B5L180_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3H</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L180"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003636"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003675.1">
        <property type="protein sequence ID" value="ENSXCOP00000003636.1"/>
        <property type="gene ID" value="ENSXCOG00000002856.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166389"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038191">
        <property type="term" value="F:neuropilin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001712">
        <property type="entry name" value="Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000008">
        <property type="entry name" value="semaphorin-3B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF21">
        <property type="entry name" value="SEMA DOMAIN, IMMUNOGLOBULIN DOMAIN (IG), SHORT BASIC DOMAIN, SECRETED, (SEMAPHORIN) 3H ISOFORM 2 PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="5">
        <location>
            <begin position="24"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="575" mass="65176" checksum="8B960CC779F55A02" modified="2018-12-05" version="1">MLVHLLKFQPSLPTLGLMLYLCHMLFFFFSTAELIQNGRLLTLPLEAGDLHSLLPDEDRRRLYVAMKDNLLSTSLDDITQNPRKLFWPASPDRVQECLMAGKDSELDCANFLRVLQPFNQTHLYVCGTGAFNPRCAFIATSIFHQAEYQTLSYSQTECGKGKCPYDPFQKTASAVVDGELYAGITSDFMSRDSAFFRSLGRRHVIRTEQYDSTWLQDAQFVRVAPLSETDNPEDDKVYVFFTERAQEAEGAAGKVLYSRVARVCKNDIGGQRSLVNKWSTFQKARMVCSVPGPDGLQTHFDQLQDIFILHGKDKKNPLIYGLFTTSSDILNGSAVCVYRMEDVVRAFKGNFLHKEGPQYKWAEFTGKVPYPRPGTCPSSTYGSYSSTREYPDDVIFFSRTHPLLQEHVLPLGERPLLVRVGVHYKFSKLLVDRVEAVDGTYDVLFIGTDSGLVLKAIHLPREHGQSQEVTLEQLQVFQHKSPVTAMALSKKKWLFVGSREGVSQLALYQCELYGQACAECCLARDPYCTWDGHACSPYMPTVRRRNARHLGEEEDPLTQCVRQGGEEQIQRFHQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1C5</accession>
    <name>A0A3B5L1C5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1C5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003731.1">
        <property type="protein sequence ID" value="ENSXCOP00000003691.1"/>
        <property type="gene ID" value="ENSXCOG00000002763.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164173"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF287">
        <property type="entry name" value="CADHERIN-RELATED NEURONAL RECEPTOR VARIABLE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="46"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="131"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="238"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="346"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="451"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="574"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="821" mass="90783" checksum="60EE761652E7A4C2" modified="2018-12-05" version="1">MHRPGRSAWIYLAVVLVDCYLEAVFGFLSYSVFEEVDRGSFVGNIAKDLNINIPELESRMFQIVAGSKRKYFEVNLKTGALFVNERIDREELCGNEDKCSLNVEGVINNPLKLYRFEITILDVNDNSPSFPNTLLSMNVSENTATGAKFPMNPAEDLDAGKNAVNTYKLSQNNYFSLVTHKGESVTPEIVLQKALDREKQAVIQLILIAFDGGTPAKSGSVIITVNVLDNNDNAPVFTQNLYKARVYENAEIGTSIITVNATDLDAGQNGEVVYSFSGMGRGKQTNVFEIDKITGTVTNKKNVDFEENTAFEIRVQASDSGTSPLTSHAKLLIEVLDVNDNAPEITVTSLLGTVKEDAPIGTAIALVSVFDRDSGNNAVVNCEIANDVPFKLESNYKNYYSLVVGGRLDRENAPHYNISITARDEGSPPLCSNSLVLVHVSDVNDNTPIFTESTINIFMKENNPTGEVMKKVSAVDADTDENGQVSYSLLIDNNNKLPLRSLININLETGEIICLQSFNYEELKTFQFKVQATDSGVPPLSSNVTVNVFILDENDNNPTILAPYSEHGSVNSETIPYSAESGYFVAKIRAVDADSGYNALLSYHLSEPKGNNLFRIGTSSGEIRTKRRMTLDRERAPYYNICITATDEGSPSLSTVDADIEQNGQISYSILHDNSNSLPLSSLLSINSQTGDIISLQSFNYEELKTLIAVRCHRTDSDFSRYSAPMITTHPDGSWSYSKATQQYDVCFSSDTLKSDVVVFPASFPPVDAELISINGNDTFTRTQTLPNTDKVRSLLNYCYFLLYRFYFLCISDCFKDYKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1D1</accession>
    <name>A0A3B5L1D1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pentraxin (PTX) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1D1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004658.1">
        <property type="protein sequence ID" value="ENSXCOP00000004602.1"/>
        <property type="gene ID" value="ENSXCOG00000003634.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000178560"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051005">
        <property type="entry name" value="Pentraxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45869">
        <property type="entry name" value="C-REACTIVE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45869:SF2">
        <property type="entry name" value="C-REACTIVE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51828">
        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017414104" description="Pentraxin (PTX) domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Pentraxin (PTX)" evidence="3">
        <location>
            <begin position="22"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="93" mass="10653" checksum="049BF2BB82222D09" modified="2018-12-05" version="1">KMRLSFVLVLFSISAAFSGNEAIRSLVFPSETSTSYVEMIATKPLKLTAFTLCMRVATELSGEREILLFAYRTNDFDELNVWRDLKTKYFAQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L262</accession>
    <name>A0A3B5L262_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dopamine beta-hydroxylase</fullName>
            <ecNumber evidence="7">1.14.17.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Catalyzes the hydroxylation of dopamine to noradrenaline (also known as norepinephrine), and is thus vital for regulation of these neurotransmitters.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>dopamine + 2 L-ascorbate + O2 = (R)-noradrenaline + 2 monodehydro-L-ascorbate radical + H2O</text>
            <dbReference type="Rhea" id="RHEA:19117"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="ChEBI" id="CHEBI:59513"/>
            <dbReference type="ChEBI" id="CHEBI:59905"/>
            <dbReference type="ChEBI" id="CHEBI:72587"/>
            <dbReference type="EC" id="1.14.17.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:19118"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Catecholamine biosynthesis; (R)-noradrenaline biosynthesis; (R)-noradrenaline from dopamine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer; composed of two disulfide-linked dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle</location>
            <location evidence="3">Chromaffin granule lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Chromaffin granule membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the copper type II ascorbate-dependent monooxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.17.1" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L262"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006398"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006478.1">
        <property type="protein sequence ID" value="ENSXCOP00000006398.1"/>
        <property type="gene ID" value="ENSXCOG00000004955.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063085"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034466">
        <property type="term" value="C:chromaffin granule lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042584">
        <property type="term" value="C:chromaffin granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004500">
        <property type="term" value="F:dopamine beta-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042420">
        <property type="term" value="P:dopamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042421">
        <property type="term" value="P:norepinephrine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006589">
        <property type="term" value="P:octopamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030431">
        <property type="term" value="P:sleep"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd09631">
        <property type="entry name" value="DOMON_DOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.310:FF:000003">
        <property type="entry name" value="Dopamine beta-hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.230:FF:000001">
        <property type="entry name" value="Monooxygenase, DBH-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.310">
        <property type="entry name" value="Copper type II, ascorbate-dependent monooxygenase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014784">
        <property type="entry name" value="Cu2_ascorb_mOase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000323">
        <property type="entry name" value="Cu2_ascorb_mOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036939">
        <property type="entry name" value="Cu2_ascorb_mOase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024548">
        <property type="entry name" value="Cu2_monoox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000945">
        <property type="entry name" value="DBH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045266">
        <property type="entry name" value="DOH_DOMON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008977">
        <property type="entry name" value="PHM/PNGase_F_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028460">
        <property type="entry name" value="Tbh/DBH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10157">
        <property type="entry name" value="DOPAMINE BETA HYDROXYLASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10157:SF29">
        <property type="entry name" value="DOPAMINE BETA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03712">
        <property type="entry name" value="Cu2_monoox_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01082">
        <property type="entry name" value="Cu2_monooxygen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03351">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00767">
        <property type="entry name" value="DBMONOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00664">
        <property type="entry name" value="DoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49742">
        <property type="entry name" value="PHM/PNGase F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50836">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0127">Catecholamine biosynthesis</keyword>
    <keyword evidence="16" id="KW-0186">Copper</keyword>
    <keyword evidence="21" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="18 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="10 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0847">Vitamin C</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="DOMON" evidence="25">
        <location>
            <begin position="51"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00020179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00037327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00047952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="598" mass="67629" checksum="0B19F889C8B53241" modified="2018-12-05" version="1">MRIFSKDVRLQDITIMYFTGLAALMVILVASYQASLPPLPMPFRMPLDPLGNLQLAWNISYATQEIYIQLRVAELRHGVVLGMSDRGELTNADLVVLWDNGTRSYFGDAWSDSKGHVSLDSHQDYELIEAKQKADGFYLLFKRPFSTCDPRDYVIEEGTVHIIYGLLDQPLASLGDLNLSRIHMGVQRVLMLRPDMPSPTLPPDVKTMDVLAPNVIIPNQETTYWCFIQKLPDDMPKNHIVMYESVITPGNEAIVHHIEVFECAPDLREVPQYSGSCDDKMKPKKLNFCRHVLAAWAMGAEAFYYPPDAGLAIGGPGSSRFLRLEVHYHNPLLIAGRHDSSGIRLHYTPNLRRYDAGIMELGLVYTPVMAIPPKQHTFYLTGYCTSKCTRTALPPGGIYVFASQLHTHLAGRGVRTVLVRGGKELEVVQEDQHFSTDYQTIRVLKKMINILPSDVLITKCTYNTEDRSKPTVGGFGIMEEMCVNYIHYYPRTQLELCKSHVDPGYLQKYFSFINRFHGNDQCVCGDFGVTDQYAQLHWDDFAGEVLDSLYNTAPISMHCNQSTARLFPGEWEKQPLPEVTTILPKHRYPCEGGAESYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2X8</accession>
    <name>A0A3B5L2X8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">pancreatic elastase II</fullName>
            <ecNumber evidence="10">3.4.21.71</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Preferential cleavage: Leu-|-Xaa, Met-|-Xaa and Phe-|-Xaa. Hydrolyzes elastin.</text>
            <dbReference type="EC" id="3.4.21.71"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.71" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2X8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005157"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005218.1">
        <property type="protein sequence ID" value="ENSXCOP00000005157.1"/>
        <property type="gene ID" value="ENSXCOG00000004038.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234528"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000004">
        <property type="entry name" value="Tryptase gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050850">
        <property type="entry name" value="Peptidase_S1_Elastase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24257">
        <property type="entry name" value="CHYMOTRYPSIN-LIKE ELASTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24257:SF19">
        <property type="entry name" value="CHYMOTRYPSIN-LIKE ELASTASE FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0720">Serine protease</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="12">
        <location>
            <begin position="10"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="250" mass="26820" checksum="877B8B082908D28E" modified="2018-12-05" version="1">PTYPPILSRVVGGVDVRQNSWPWQVSLQYQSGISFYHTCGGTLISDLWVITAAHCIGSRTYRVYLGKHDLTASEGGSIAISPEKIIVHPNWDSYNIRNDIALIKLASPVQLSASITPACLPTSGEILPDQAPCYVTGWGRLWTGGPIADILQQALLPVVDYPTCSRGDWWGSLVTTSMICAGGDGNVASCNGDSGGPLNCKNSDGSWDVHGVVSFGSSLGCNYPKKPSVFTRVSDYISWINNVSRSIDIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L362</accession>
    <name>A0A3B5L362_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">AFG1 like ATPase a</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AFG1 ATPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L362"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004343.1">
        <property type="protein sequence ID" value="ENSXCOP00000004296.1"/>
        <property type="gene ID" value="ENSXCOG00000003379.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013227"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000735">
        <property type="entry name" value="Lactation elevated 1 (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005654">
        <property type="entry name" value="ATPase_AFG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040713">
        <property type="entry name" value="ZapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169:SF25">
        <property type="entry name" value="AFG1-LIKE ATPASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169">
        <property type="entry name" value="ATPASE N2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03969">
        <property type="entry name" value="AFG1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="464" mass="53076" checksum="AD4B26E3CAB43D40" modified="2018-12-05" version="1">PSAALTIRSRCGDVEYPRAPGCCCCCCCWLSSLPPIAERLYESKADSTVILYVCVGPLDHYSGLIRAGSLREDEHQRAVLQKLDELHKTLRGYTNRPTSILSRFFSKPKPPQGCYIYGDVGTGKTMVMDMFYSHVETERKKRVHFHGFMLDVHKRIHRLKQSMPKRKAGKMAKSYDPIAPVAEEISEEARLLCFDEFQVTDIADAMILKQLFENLFLNGVVVVATSNRPPEDLYKNGLQRVNFVPFIAVLQKYCQTLRLDSGIDYRKRNRPSAGKLYFLSGEPDAEATLDKMFDELAFKQNDITRPRVLNVHNRKVRLNKACGTIADCTFEELCDRPLGASDYLEISRLFDTVFIRHIPLLTLNKKTQARRLITLVDALYDHKVRRRRSFALPDIFVQDGDHSHDESHILMDDLGLKRDEASSLSIFSGEEEKFAFQRTVSRLTEMQTEDYWLDGDRSLKASGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4I0</accession>
    <name>A0A3B5L4I0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L4I0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004746"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004803.1">
        <property type="protein sequence ID" value="ENSXCOP00000004746.1"/>
        <property type="gene ID" value="ENSXCOG00000003737.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154958"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001056">
        <property type="entry name" value="RAB1A, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001144">
        <property type="entry name" value="RAB1A, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="169" mass="18740" checksum="E3E925B63A1562A3" modified="2018-12-05" version="1">MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDSKTIKLQIESYNNIKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLAIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGATASGDKPNLKIESTPVRQSGGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5G3</accession>
    <name>A0A3B5L5G3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12 16">6-phosphogluconate dehydrogenase, decarboxylating</fullName>
            <ecNumber evidence="5 12 16">1.1.1.44</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 12">Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12 16">
            <text>6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:10116"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="1.1.1.44"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12 16">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12 16">Belongs to the 6-phosphogluconate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.44" evidence="5 12 16"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5G3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006052"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006122.1">
        <property type="protein sequence ID" value="ENSXCOP00000006052.1"/>
        <property type="gene ID" value="ENSXCOG00000004692.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009023"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00410"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004616">
        <property type="term" value="F:phosphogluconate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019521">
        <property type="term" value="P:D-gluconate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000002">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.320:FF:000002">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000007">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006114">
        <property type="entry name" value="6PGDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006113">
        <property type="entry name" value="6PGDH_Gnd/GntZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006184">
        <property type="entry name" value="6PGdom_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006183">
        <property type="entry name" value="Pgluconate_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00873">
        <property type="entry name" value="gnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006765">
        <property type="entry name" value="PRK09287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11811">
        <property type="entry name" value="6-PHOSPHOGLUCONATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00393">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000109">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00076">
        <property type="entry name" value="6PGDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01350">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00461">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0311">Gluconate utilization</keyword>
    <keyword evidence="7 12 16" id="KW-0521">NADP</keyword>
    <keyword evidence="8 12 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10 12 16" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase C-terminal" evidence="17">
        <location>
            <begin position="202"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="32"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="55"/>
            <end position="57"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="97"/>
            <end position="99"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="151"/>
            <end position="153"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <begin position="209"/>
            <end position="210"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="14">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
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        <location>
            <position position="283"/>
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        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
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        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
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        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="475"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018193"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00048640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="505" mass="55776" checksum="17A1DC3FC467F93A" modified="2018-12-05" version="1">CFLFTRKRFTSLLSSFEEIFANDARADIALIGLAVMGQNLIMNMNDHGFVVCAYNRTVSKVHDFLQNEAKGSKVVGAESLEDMVSKLKKPRRIILLVKAGQAVDDFIDKLVPLLQAGDIVIDGGNSEYRDTTRRCKSLKEKGLLFVGSGVSGGEEGARYGPSLMPGGHKEAWPHIKDIFQSIAAKVGTGEPCCDWVGDEGAGHFVKMVHNGIEYGDMQLICEAYHLMKDVLGMDHDEMAQAFDSWNKTELDSFLIEITANILKYKDADGTHLLPKIRDSAGQKGTGKWTAISALEYGTPVTLIGEAVFARCLSSLKEERVQASRSLSGPQGVKFNGNKAAFLEDIRKALYASKIISYAQGFMLLRQAAKEFGWTLNYGAIALMWRGGCIIRSVFLGKIKEAFDRDADLQNLLLDNFFSNAVQDCQESWRRTVCAGVQHGIPMPCFTTALSFYDGYRHDMLPANLLQAQRDYFGAHTYELLSNPGHYIHTNWTGHGGNVSSSSYNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6T2</accession>
    <name>A0A3B5L6T2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Scaffold protein ILK</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">ILK-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">ILK-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Inactive integrin-linked kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">p59ILK</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Myofibril</location>
            <location evidence="2">Sarcomere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6T2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008018"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008116.1">
        <property type="protein sequence ID" value="ENSXCOP00000008018.1"/>
        <property type="gene ID" value="ENSXCOG00000006161.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155956"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030017">
        <property type="term" value="C:sarcomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034446">
        <property type="term" value="P:substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14057">
        <property type="entry name" value="PK_ILK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000187">
        <property type="entry name" value="integrin-linked protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000245">
        <property type="entry name" value="Integrin-linked protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035692">
        <property type="entry name" value="PK_ILK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF57">
        <property type="entry name" value="INTEGRIN-LINKED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="15">
        <location>
            <begin position="33"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="135"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="394" mass="45113" checksum="1027E6925088AD60" modified="2018-12-05" version="1">MDDIFTQCREGNAVAVRLWLDNTENDLNQGDDHGFSPLHWACREGRSNVVDMLIMRGARINVMNRGDDTPLHLAASHGHRDIVVKVGVHVNTKRYFYDYISGQTFLKMVIFSLAQRFKRRNGTLNKLAGIDHRQLSFTEKRNENHSGELWQGRWQGNEIAVKVLKIRDWTPRKSRDFNEEYPKLRIFSHPNVLPMLGACPSPPAPHPIIITHWMPCGSLYKVLHEGTNFVVDQTQAVKFALDIASGMAFLHTLEPMIPRHHLNSKSIMIDEDLTARISMADVKFSFQCPGRMYSPAWVAPEALQKKPEEINRRSADMWSFAILLWELVTREVPFADLSNMEIGMKIALEGLRPTIPPGISPHICKLMKICMNEDPAKRPKFDMIVPILDKMQDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7D7</accession>
    <name>A0A3B5L7D7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="8">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L7D7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006350"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006428.1">
        <property type="protein sequence ID" value="ENSXCOP00000006350.1"/>
        <property type="gene ID" value="ENSXCOG00000004905.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165673"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044322">
        <property type="term" value="C:endoplasmic reticulum quality control compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904380">
        <property type="term" value="P:endoplasmic reticulum mannose trimming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044674">
        <property type="entry name" value="EDEM1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45679">
        <property type="entry name" value="ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0834">Unfolded protein response</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017208828" description="alpha-1,2-Mannosidase" evidence="10">
        <location>
            <begin position="36"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="11">
        <location>
            <begin position="634"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="768"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="768"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="795"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="365"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="831" mass="92520" checksum="24B6A426F0D78784" modified="2018-12-05" version="1">MRLGAKSQRKPGLCFGTDMLLKTLLITSLLSWVNCLENQSMTPEEKTNIRNQILEMFDHAYGSYMKYAYPADELMPLSCKGRVRCFLLVACAQVLNKLDEFDDAVKKAVRDVRLDNDVVVSVFETNIRVLGGLLGGHVMADLLRQRGERMQWYRDELLHMAVELGHRLIPAFNTSSGLPYPKVNLRYGILNPLSRTGTESDTCTACAGTMILEFAALSRLSGESVFEEHARKALDVLWERRQRGSDLVGTVINIHNGEWVRKDSGVGAGIDSYYEYLMKAYILLGDNTFLQRFNIHYSAIMKYISQPPLLLNVHMHNPTVSVRSWMDSLLAFFPGLQVLTGDLKPAIETHEMLYQVTKQHKFLPEAFTTEFRVHWGQHLLRPEFAESTYYLFKATGDPYYLRVGQSIVEKLNAFARVPCGFAAVQDVRTGTHEDRMDSFFLAEMFKYLYLLFSEKSQLPINMDNYIFTTEAHLLPVSLSTTQVTVQTNVTEAPVAHDEDLFTHSCPSTETLFPNNPSFAKTIRDGYKYLTGVGRTLSISLPLHDSGMEPVEFLKSMGISLTPLNDFTLGDSIGSKQHKGVFRVKLVAEVSQTIEEELVVPHAVQLISPPFLGRTVLTAGPAKFGMDLTKQEHGVKGTIVKASPYTACGPIENAAEVKGRIALALRGDCMFAAKARWLQQAGAGGVIFIDHREGSNSEETPLFQMVGDGDSTEDIALPLVFLFSREGAVLTSALEEHHNVDVLLLPKERQLGQDKSENPVSMNIKLRLREEGGSEDRAAPGATLELVLEDEEELSGKQQSPHQQVCTQGRTGPCSTDSPQALNSKPEPGTNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7E2</accession>
    <name>A0A3B5L7E2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase M12B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L7E2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008223"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008321.1">
        <property type="protein sequence ID" value="ENSXCOP00000008223.1"/>
        <property type="gene ID" value="ENSXCOG00000006309.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159790"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04269">
        <property type="entry name" value="ZnMc_adamalysin_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034027">
        <property type="entry name" value="Reprolysin_adamalysin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF114">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M12B" evidence="4">
        <location>
            <begin position="74"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="19"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="270" mass="30522" checksum="F1F5894CD7C83D2A" modified="2018-12-05" version="1">MTSHFSAFVRLAGCFMNSTEPEGRTDGLSEGDSEIKDGDNKSEEEKTVFTRGQRRSKRQVSVRRGQRTVQTETKYIELMIVNDYELLRRSTSQTKIFAKSVVNMADLIYKEQLNTRIVLVAMETWSSENRIAVGDDALLTLRDFMKFRKESIKQRCDAVHLFSGRTFMSSRSEAAYIGGICSLTKGGGINEFGSVGPMAITLCQSLGQNIGMLRNKERTAAGNSSLILVESFKNKMADTQENDSDLTLRDVTHSWLFFFYFKTIYIPFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L895</accession>
    <name>A0A3B5L895_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L895"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007130.1">
        <property type="protein sequence ID" value="ENSXCOP00000007042.1"/>
        <property type="gene ID" value="ENSXCOG00000005442.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155231"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045180">
        <property type="term" value="C:basal cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052212">
        <property type="entry name" value="PH-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12156:SF30">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY-LIKE DOMAIN FAMILY B MEMBER 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12156">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY-LIKE DOMAIN, FAMILY B, MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="189" mass="20980" checksum="C16ABCA1220B90C4" modified="2018-12-05" version="1">RDRLNKNLLAYFFFTVWFPIFNVTLQHYKCDFLVFFLAGCMLCFGQSAFFRFNHPEEAFRMKSMIPHGGGVRTGSYALCPDTESLVNGNHQASSSQSPSAPGERVQSEHSAIVSSIEKDLQEIMDSLVMDDLEPSSSDAKKPPGGQSMHHSPLSPMVNGGGRYLLSPPTSPGSFVCLLQIVFYHKNLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8F1</accession>
    <name>A0A3B5L8F1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8F1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008573"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008679.1">
        <property type="protein sequence ID" value="ENSXCOP00000008573.1"/>
        <property type="gene ID" value="ENSXCOG00000006563.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286991"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Immunoglobulin V-set" evidence="1">
        <location>
            <begin position="31"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="116" mass="12423" checksum="9140A2436DAD8F16" modified="2018-12-05" version="1">MSRVSSLSSGSDGQNVVVTQSAAKSVQLGQTVTIDCKASVQLTGCSTPCLSWYHHKPGEVPKALIYWTSSRFSGISSRFSGSGAGNKLDFTLTISGVQAEDIYFVFIYCTFCANHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8V2</accession>
    <name>A0A3B5L8V2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">EPH-related receptor tyrosine kinase ligand 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 14">Belongs to the ephrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8V2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008728"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008834.1">
        <property type="protein sequence ID" value="ENSXCOP00000008728.1"/>
        <property type="gene ID" value="ENSXCOG00000006666.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157299"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046875">
        <property type="term" value="F:ephrin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022407">
        <property type="term" value="P:regulation of cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10425">
        <property type="entry name" value="Ephrin-A_Ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000005">
        <property type="entry name" value="Ephrin A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031328">
        <property type="entry name" value="Ephrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034252">
        <property type="entry name" value="Ephrin-A_Ecto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019765">
        <property type="entry name" value="Ephrin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001799">
        <property type="entry name" value="Ephrin_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304">
        <property type="entry name" value="EPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304:SF33">
        <property type="entry name" value="EPHRIN-A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00812">
        <property type="entry name" value="Ephrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01347">
        <property type="entry name" value="EPHRIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01299">
        <property type="entry name" value="EPHRIN_RBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51551">
        <property type="entry name" value="EPHRIN_RBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 14 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017463887" description="EPH-related receptor tyrosine kinase ligand 7" evidence="16">
        <location>
            <begin position="22"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Ephrin RBD" evidence="17">
        <location>
            <begin position="30"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="63"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="216" mass="25497" checksum="D07D885131ABB43D" modified="2018-12-05" version="1">VMLQVEMVLFVCLVLLMCVLSQEPGSKVVADRYAVFWNRTNAKFHRGDYHIDVCINDYLDVYCPHYEDSVPEERTERYVLYMVNYDGYSTCDHTAKGFKRWECNRPHSPNGPLKFSEKFQLFTPFSLGFEFRPGREYYYISFHTIPVSHRKLFFFPKMDVEFIGESKLRRFESYSSLSPSCTKPHLKKEGPSVCLSSAFILCLLFCLWYMLRCLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L944</accession>
    <name>A0A3B5L944_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L944"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006985"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007073.1">
        <property type="protein sequence ID" value="ENSXCOP00000006985.1"/>
        <property type="gene ID" value="ENSXCOG00000005397.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154981"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000064">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000066">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000067">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026">
        <property type="entry name" value="FAT ATYPICAL CADHERIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026:SF126">
        <property type="entry name" value="PROTOCADHERIN FAT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="8">
        <location>
            <begin position="40"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="8">
        <location>
            <begin position="156"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="8">
        <location>
            <begin position="372"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="8">
        <location>
            <begin position="468"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="8">
        <location>
            <begin position="592"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="8">
        <location>
            <begin position="722"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="805" mass="90037" checksum="F94B5261E33A4328" modified="2018-12-05" version="1">PFSYLPVVSEITKQAMLLELTVPWGDCLEEAFKEKVSSFTHSVYHATVYENSAARTYANSKIKMGIHLTKRSWEIRYRITSGDDEGLFKAEDYVLGDFSFLRIRTKGGNAAILNREIQDNYVLTVKAYVKGESLLEAWTKVSIQVLDMNDLRPLFSPTTYSVTIAESTPLRTSIAQVTATDADIGSNGEFYFFFKDKMELFAVHPTSGVVYLSGKLNVDEQNRYDLEIMAVDRGMKLYGNNGVSSTAKLFVHIERVNEHAPVMTVVTHASSRLEKNSLYALVSVEDFDEGLNGEIDSVTIMDGDPSGQFYIERREEEEFAIKSTEMINWETFPYGCNLTLQAKDRGFPPQFSDPQVLHIMFSNQQTTEANFEQEFYVVSLNEVSPPGTIVGFFKIKPEPDDAEYILTPSADSTFFKINTLTGVISTSRWLTHVSKDIFSLEVLEVDSNLRAKVQVAIEDANDNTPEFSQSFYEVFVNESVPAGTNVLTVSAVDDDKGENGYITYSIHCLQQLPFKINQFSGVISSNTDLDFEASPEVFVFVVRASDWGSPYRRESEVNVTIHLQNVNDNKPLFEKVACKGVISVDFPVDEAITTMSAIDIDELELVKYKIISGNELGLFDLNPDSGVLALRNSLSALGPKHSIFSLKITSTDGENLSDPMFVNISVVHGKSLYKHFTCKETSVAQRLAEKLLTKSKASTKSKTEEGFIDLFSVNRQTPQFDKAFPTDIAVQEDLKVGSSVFQVNAYDDVSVIQIHAQDPDVSPPSTADWLSYRIVSGNPQNFFTINSRTGESFSVSRSSEKDFEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9E2</accession>
    <name>A0A3B5L9E2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Solute carrier family 12 member 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SLC12A6</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) + chloride(in) = K(+)(out) + chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:72427"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the SLC12A transporter family. K/Cl co-transporter subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9E2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008999.1">
        <property type="protein sequence ID" value="ENSXCOP00000008893.1"/>
        <property type="gene ID" value="ENSXCOG00000006781.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160238"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015379">
        <property type="term" value="F:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055064">
        <property type="term" value="P:chloride ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055075">
        <property type="term" value="P:potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000049">
        <property type="entry name" value="Solute carrier family 12 (potassium/chloride transporter), member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000040">
        <property type="entry name" value="Solute carrier family 12 member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000076">
        <property type="entry name" value="KCL_cotranspt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018491">
        <property type="entry name" value="SLC12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004842">
        <property type="entry name" value="SLC12A_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00930">
        <property type="entry name" value="2a30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827:SF66">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12 MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03522">
        <property type="entry name" value="SLC12"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01081">
        <property type="entry name" value="KCLTRNSPORT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="149"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="357"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="402"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="448"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="502"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="526"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="559"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="18">
        <location>
            <begin position="76"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="18">
        <location>
            <begin position="360"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="19">
        <location>
            <begin position="655"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="19">
        <location>
            <begin position="788"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="19">
        <location>
            <begin position="975"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="905"/>
            <end position="934"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF03522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1045" mass="115895" checksum="665004AF87D869CB" modified="2018-12-05" version="1">VYINNSHGMDDDEFFDRNLALFEEEMDTRPKVSSLLNRLANYTNLTQGAKEHEEAESIGEKKKASKSPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGVLQALCIVFTCCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGICFYLGTTFAGAMYILGAIEIFLMYIVPQAEIFKGGGAAMLNNMRVYGSICLLLMSLLVFVGVKYVNKLASIFLACVIVSIVSIYIGAFVSAFYEHKFDVCMLGNRTISSHVECSKTVPVGIKDTEKTTLLWEEFCHSTELNATCDEYFTSNNFTKIKGIPGLASGIISENLWSSYISKGEVLQKSSLSSSHSSPPASLPHPYVFADITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQRSIPIGTILAILTTSFVYLSSVVLFGACIDGVVLRDKFGESVDGTLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKANGEPTWALLLTALIAELGILIASLDLVAPILTMFFLMCYLFVNLACALQTLLRTPNWRPRFSYYHWTLSFLGMIICLALMFISSWYYAIVAMVIAGMIYKYIEYHGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDAHVKSPRLLTFASQLKAGKGLTIVGTVVPGHFLQTYGEALAAEQTLKLLMEKERVKGFVQCIVAQKPREGISHMIQSSGLGGMKPNTVVMGWPHTWRQSEDPQAWKTFINTVRVTTAAHLALLVPKNISLFPSNSEPPTDGYIDVWWIVHDGGMLMLLPFLLRQHKVWRKCGMRIFTVAQMEDNSIQMKKDLATFLYHLRIEAEVEVVEMHNSDISAYTYERTLMMEQRSQMLRQMRLSKSDREREAQLVKDRNSMLRLTSIGSDDEDDTDGGERERPVSISSEHHRRVQMTWTKEKTAQYRAAHSGCSTPEGFRDMLSIRPDHSNVRRMHTAVKLNEVIVNKSHDARLVLLNMPGPPKNTDGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LB11</accession>
    <name>A0A3B5LB11_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dipeptidyl-peptidase IV</fullName>
            <ecNumber evidence="3">3.4.14.5</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.</text>
            <dbReference type="EC" id="3.4.14.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S9B family. DPPIV subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.5" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LB11"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009499.1">
        <property type="protein sequence ID" value="ENSXCOP00000009388.1"/>
        <property type="gene ID" value="ENSXCOG00000007149.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158174"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.140.10.30:FF:000002">
        <property type="entry name" value="Dipeptidyl peptidase 8-like isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045785">
        <property type="entry name" value="Dpp_8/9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF193">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19520">
        <property type="entry name" value="Dpp_8_9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017438408" description="dipeptidyl-peptidase IV" evidence="8">
        <location>
            <begin position="23"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidyl peptidase 8 /9 ,N-terminal" evidence="10">
        <location>
            <begin position="38"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="9">
        <location>
            <begin position="146"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF19520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="471" mass="54271" checksum="0B4690B505FB2A86" modified="2018-12-05" version="1">MLTGSWYCCCSCWTVSMMAVDGLWDSTEVVEMGDVPSQFFVEKHSWEGLRDIIHCSRKYSGMIANKAPHDFQFVQKTDDNGPHSHRLYYLGMPYGSRENSLLYSEIPKKVRKEALLVLSWKQMLDHFQRNCANTINKTITVRIHVHYITTAYLFQQSAPVKPVEIKSQCSGTRMDPKICPGHPDFIAFINNNDLWIANIKTGEERRLTYCHKGLDNVKEDPRSAGVATFVIQEEFDRFTGYWWSPFAAADPDGGKTLHLLYEEVDETEVEIIHVPSPALEERKADAYRYPRTGSKNPQATLKLAEIRMDHQGKIISTQDKELVVPFSTLFPGTEYIARAGWTIDGKYGWAVLLDRSQRKLQLVLLPPALFIPITEDSTLRQDSLEAVPSCTQPYIIYEEATDVWINVHDIFYPFIQTTTEDEFTFIWVNESKTGFSHLYKITSLLQPGYHHWTEEYHHVEGEKKCFCITQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LB58</accession>
    <name>A0A3B5LB58_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Transmembrane protein 230</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Involved in trafficking and recycling of synaptic vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle</location>
            <location evidence="5">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle</location>
            <location evidence="3">Synaptic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus</location>
            <location evidence="6">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TMEM134/TMEM230 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LB58"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006951"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007039.1">
        <property type="protein sequence ID" value="ENSXCOP00000006951.1"/>
        <property type="gene ID" value="ENSXCOG00000005377.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008694"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048489">
        <property type="term" value="P:synaptic vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044234">
        <property type="entry name" value="TMEM230"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008590">
        <property type="entry name" value="TMEM_230/134"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15664">
        <property type="entry name" value="C20ORF30 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15664:SF6">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05915">
        <property type="entry name" value="TMEM_230_134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="9 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00007743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="117" mass="12878" checksum="FF74F8CEF503D963" modified="2018-12-05" version="1">ALPSRRTGLSNNKAKYSRLAADDDGYIDLQFKKSPPKIPYKAIGVAVILFLIGSLLIILGALFLAGIINVENPDRTIPVIVIGLLVFLPGFYHLRIAYYAAKGYRGYSYDDIPDFGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCC6</accession>
    <name>A0A3B5LCC6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Serine/threonine kinase 32C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">STK32C</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCC6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008492"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008598.1">
        <property type="protein sequence ID" value="ENSXCOP00000008492.1"/>
        <property type="gene ID" value="ENSXCOG00000006510.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160573"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004703">
        <property type="term" value="F:G protein-coupled receptor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05578">
        <property type="entry name" value="STKc_Yank1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000169">
        <property type="entry name" value="Serine/threonine-protein kinase 32A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000158">
        <property type="entry name" value="Serine/threonine-protein kinase 32A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF32">
        <property type="entry name" value="SERINE_THREONINE KINASE 32C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="42"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="396" mass="45689" checksum="01724800758BCAE2" modified="2018-12-05" version="1">HLPGPRLHLQTCTPGSNLNQEHGMPGGGRRVEAHHLLNFDHFQILRAIGKGSFGKVCIVQKRDTEKMYAMKYMNKQQCIERDEVRNVFRELEILQEIEHVFLVNLWYSFQDEEDMFMVVDLLLGGDLRYHLQQNVQFGEDAVKLYLCEMTLALDYLRSQHIIHRDVKPDNILLDEQGHAHLTDFNIATIIKDGERATALAGTKPYMAPEIFQSFVSGGTGYAFEVDWWSLGVTAFEVLRGWRPYDIHASNSVESLIQLFSTVSVQYSPAWPKDLVALLRKLLTVNPEHRFSSLSDMQTSPYLAAVNWDAVYEKKMEPGFVPNKGRLHCDPTFELEEMILESRPLHKKKKRLAKNRSRDNSKDSQSVSHCFYMFDQICETIVVFNCAGLEQSYKINW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDQ7</accession>
    <name>A0A3B5LDQ7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Polymeric immunoglobulin receptor</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDQ7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010298"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010421.1">
        <property type="protein sequence ID" value="ENSXCOP00000010298.1"/>
        <property type="gene ID" value="ENSXCOG00000007807.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182977"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="IgV_pIgR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF87">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Immunoglobulin V-set" evidence="4">
        <location>
            <begin position="22"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="200" mass="22486" checksum="B3D3B8DD86907C6A" modified="2018-12-05" version="1">RIKLVLSIHHIPFLPAFLCKVTTEEEFAVLEGGSLTIPCHYEPQYASYVKYWCQGRTREFCTSLARTDDHHLVDPAEKKVSIFDDPVQLVFTVTMTDMKEGESGWYICGVEIGGVWEADDVAYTDVKVVHGMSAVNSRMSGAEGSAVTIECLYSERYRHLTFSPLTKKASFLLCSSMENLIDVLKIFSVQTSTDHCITQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LE38</accession>
    <name>A0A3B5LE38_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Fidgetin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027880197.1">
        <property type="nucleotide sequence ID" value="XM_028024396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LE38"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010423"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010546.1">
        <property type="protein sequence ID" value="ENSXCOP00000010423.1"/>
        <property type="gene ID" value="ENSXCOG00000007897.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114148889"/>
    <dbReference type="CTD" id="55137"/>
    <dbReference type="GeneTree" id="ENSGT00940000157526"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008568">
        <property type="term" value="F:microtubule severing ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19523">
        <property type="entry name" value="RecA-like_fidgetin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000022">
        <property type="entry name" value="Fidgetin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000495">
        <property type="entry name" value="Fidgetin like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047828">
        <property type="entry name" value="Fidgetin_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF14">
        <property type="entry name" value="FIDGETIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="501"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="133"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="180"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="238"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="322"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="741" mass="80015" checksum="27C8BA9905657E00" modified="2018-12-05" version="1">MQWTPEHTQWAEQHFDISSTTRSPAHKVEAYRGHLQRTYQYAWANDDISALTASNLLKKYAEKYSGILEGPNERALLCAYSDSSSGLVNGRKSENESWQEGIYPMNCAPDVISVSKAGMTAALPPTDLSASIGSSPGVASSLSEPSYSSSNCGSHPATTLHSGLPSQEYTASYNGSYLHSSYSSQSTPALPSPHPSPLQSAGLLQPPPPPPALVPSYNAGSPNLPNYSYPPTGYASQTAVGPSYSPGGAPPPPSAYLPSGIAAPTPIPPSTLPGYTYQSHNHTPITPTPLNGSSSNSLKRKAFYMSGHGDVDSSYGNFNYNQQRSSQSPMYRVADSSVSDSNRGTGFERNTEPSSLAFKPTKQQLASDQQRKFNVHSGRALTPPSYGSTKNSAGDLRTGEPYSKFGSPIMSEQTDEHRQHLSHSLPGPDLGTATSSIHAAEEQLKNSDSNLVEMVNTEILQQGSPVDWTDIAGLDMAKSTIKEEILWPILRPDMFSGLSTLPRSLLLFGPQGTGKSLLARCMASQLGAAFLRLSGSALVTKWLGEGGKIIQASFLVARCRQPAVVLISDVDLLLSGQLTEESPVNHLKAELLIQLDGILTSAEDHVLVVCSTSKPEEIPETLRRYFTKRLLIPLPDGTARHQIISQLLSQHNYCLSDKEISLLVQRTEGFSGLDVVQLCQEAVVGSLHGIPGADLPSIHPSQMRPVTYQDFENVFCKFQPSISQKELDVYTEWNKMFGCSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEA9</accession>
    <name>A0A3B5LEA9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LEA9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010493"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010616.1">
        <property type="protein sequence ID" value="ENSXCOP00000010493.1"/>
        <property type="gene ID" value="ENSXCOG00000007937.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286977"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003862">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001235">
        <property type="entry name" value="Si:ch211-119o8.6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000703">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001158">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000562">
        <property type="entry name" value="Zinc finger protein 786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF15">
        <property type="entry name" value="IP01243P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="284"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="343" mass="39625" checksum="E010D2982FACE0BF" modified="2018-12-05" version="1">MGTKQELDPVLIKEEPESIQIKEQEEPILVKIKEEPELVHIKEEPELIKVEHEDLDSEQEEKQLIVKQETETCAASPTYKIHTNKKPFLCLTCGKGFIRQPDLKVHMTHHTGEKPFQCLTCGKAFPRQPDLTVHVRTHTGEKPFQCLVCGKGFIQKTTMLCHMRTHTGEKPFQCLDCGKGFIQKTTLRYHMRTHTGEKPFSCELCDKSFRQRSDLACHIKTHTDEKPHRCEKCGKSFRKRSDLANHTLSHTGEMPFPCLTCGKGFKQQYKLTMHMKVHTGDKPYNCELCDKSFRQRSDLTRHLKTHKRVKLSSCTVCGKLLSEFSSMPRHMKTHTGEKASRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEE2</accession>
    <name>A0A3B5LEE2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LEE2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008154.1">
        <property type="protein sequence ID" value="ENSXCOP00000008056.1"/>
        <property type="gene ID" value="ENSXCOG00000006189.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154395"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF32">
        <property type="entry name" value="BLOODTHIRSTY-RELATED GENE FAMILY, MEMBER 16 ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="25"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="139"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="176"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="262" mass="29393" checksum="30D0F76AAF617F88" modified="2018-12-05" version="1">MKPFCTGCLLNQTASPNPMSTPADCCICLDEFTSPASLPCGHRFCLACIGEYWRTGESCQCPLCMAVFPKRPQLKTERTENGAEPLKAGEVPCDVCPARRAAVRSCLVCLASYCSAHLEPHYQREDLGRHLLVSVAKNLEDSVCRLHGRKLDKFCRSDRSCICATCARTEHRGHHVVSVSKEATKLKRRRLKLQQEIQEKLSVAEDAERTAGLRGGELPAELWAQARKPLKLLEEEISELQERNAELEQLLQTEDNLRFLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LF88</accession>
    <name>A0A3B5LF88_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LF88"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010828"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010953.1">
        <property type="protein sequence ID" value="ENSXCOP00000010828.1"/>
        <property type="gene ID" value="ENSXCOG00000008178.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156162"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:recycling endosome"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF57">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE IF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="2">
        <location>
            <begin position="35"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="1">
        <location>
            <begin position="123"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="206" mass="23606" checksum="C50608861CF560C2" modified="2018-12-05" version="1">SWLWLHQCGFDPPHQSDTRTVYVANRFPEHGHYIPQRFADNRIISSKYTIWNFVPKNLFEQFRRIANFYFLIIFLVQLMIDTPTSPVTSGLPLFFVITVTAIKQGYEDWLRHKADNEVNGAPVFVVRSGSLVQTRSKNIRVGDIVRVAKDETFPADLVLLSSDRADGTCHITTASLDGETNLKVCLTDRSVQIYLYCSFLFIPCLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFV5</accession>
    <name>A0A3B5LFV5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Atonal bHLH transcription factor 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFV5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006744"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006827.1">
        <property type="protein sequence ID" value="ENSXCOP00000006744.1"/>
        <property type="gene ID" value="ENSXCOG00000005220.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160064"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042668">
        <property type="term" value="P:auditory receptor cell fate determination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060119">
        <property type="term" value="P:inner ear receptor cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035675">
        <property type="term" value="P:neuromast hair cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048923">
        <property type="term" value="P:posterior lateral line neuromast hair cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd19713">
        <property type="entry name" value="bHLH_TS_ATOH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000025">
        <property type="entry name" value="protein atonal homolog 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032661">
        <property type="entry name" value="ATOH1_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF82">
        <property type="entry name" value="TRANSCRIPTION FACTOR ATOH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="9">
        <location>
            <begin position="143"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="75"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="260"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="316"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="325" mass="35247" checksum="A2B6B12E94261BD5" modified="2018-12-05" version="1">MDVLTVEEWSKGAREVAALGALQQERVSTGVVERGGNHQLQVSRYDPGHTLVDGGSDPRAWLTDASGTCAAHATSPDYLRCSPGSSGHPSPPIHRRSAKSPSSSSLKVRDLCRLKGTITGPEEEAPLRQRAQSSKPVSGVQRQRRVAANARERRRMHGLNHAFDELRSVIPAFDNDKKLSKYETLQMAQIYINALAELLQGPVSTNNENSDGSPKRDILLSPADGFEGAKERAAQSPSLTCRTAVALPLSENSFTGRVEKAMCSPSSSSSLVGNGRKESPRSDGEFSPRSHFSDSDEIAMELHSSEEDDLSEIKLPSHRHPTVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJH1</accession>
    <name>A0A3B5LJH1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJH1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009875.1">
        <property type="protein sequence ID" value="ENSXCOP00000009761.1"/>
        <property type="gene ID" value="ENSXCOG00000007416.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001437"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd10537">
        <property type="entry name" value="SET_SETD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040415">
        <property type="entry name" value="SETD9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33524">
        <property type="entry name" value="C5ORF35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33524:SF2">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="1">
        <location>
            <begin position="100"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="278" mass="31693" checksum="E9AF570E8B9F256A" modified="2018-12-05" version="1">MPGPALRRFREKWRSYRHRFVPWVVFNLSKNEKTLRRVTEPSEDKVIPDEQVSQSLLGLSRALLRNSGGLLLGAHTNFPPRFPLHREQNEGDVLFQTLGFCIERRPSSLAFAGTGVFVTRGVVPKGATVAMYPGTIYQPYEPILLQSIRNPFVFRCIDGVLVDGNDRGISRMVFRSCSGRDRMGPYLTSDASWLTDSPLNPLAVGQYVNNCSNERPANVCYQEYDVPDSFPLELRQYLPNVNYSQHCTQRPLRCVVLVSLRDIRAGEELFSNYYTIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJS3</accession>
    <name>A0A3B5LJS3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the potassium channel HCN family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJS3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011025.1">
        <property type="protein sequence ID" value="ENSXCOP00000010900.1"/>
        <property type="gene ID" value="ENSXCOG00000008234.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156523"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000031">
        <property type="entry name" value="Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000002">
        <property type="entry name" value="Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000007">
        <property type="entry name" value="Putative potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013621">
        <property type="entry name" value="Ion_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF11">
        <property type="entry name" value="POTASSIUM_SODIUM HYPERPOLARIZATION-ACTIVATED CYCLIC NUCLEOTIDE-GATED CHANNEL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08412">
        <property type="entry name" value="Ion_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0114">cAMP</keyword>
    <keyword evidence="7" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="19" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="16 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="10" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="6" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="4" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="18" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="8 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="196"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="397"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="427"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="25">
        <location>
            <begin position="528"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="765"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="811"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="91"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="765"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="783"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="822"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="844"/>
            <end position="863"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="964" mass="106474" checksum="D3D29758800BAC32" modified="2018-12-05" version="1">MKCNKNGECRRDSGTGSLRSIIPKQEKQTAGSGRAEDGGAAKRGACNSTTASMDGSITPGGSLTGGQGGESANPGATPVSSGVPEKKDSKVSFSNAPSRKASSSLHGPTQTQTQQPRNSVTFQKQEDGPPIVAAEDAEPRGSQASFMQRQFSSMLQPGVNKFSLRMYGSQKAVEKEQERVKSAGNWIIHPYSDFRFYWDFTMLLFMVGNLIIIPVGITFFKDETTTPWIIFNVVSDTFFLMDLVLNFRTGIIIEDNSDIILDPETIKKKYLKTWFIVDFISSIPVDYIFLIVEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVMRIINLIGMMLLLCHWDGCLQFLVPMLQDFPSDCWVSLNKMENDTWSELYSFAVFKAMSHMLCIGYGRQAPESLSDIWLTMLSMIVGATCYAVFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEESILGELSEPLREEIVNFNCRKLVASMPLFANAEPNFVTAMLTKLRFEVFQPQDYIIREGTIGKKMYFIQHGVCSVITKGTLAMKLSDGSYFGEICLLTRGRRTASVRAETYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILMHKVQHDLNSGVFNNRENEMIQEIVKYDREMVKLVDLQRPRAMSMTPSIGGIFAPPGQPQSGSAIATLQQAVAMSFCPQMASPLVGPGTLQSPRMVRRFQVMQNLSSPISMSPLQSQPPQTLGGAFGSAISSPPVQSPLTATGRTFQYMASVGPSGSQLSLVQHHAPSPTQTTQQRPASHKSTHSLHMGSLSQDTRALSASQPSLPQEGTPQASSAVPSPPPSTRTSNSSIGPQQPSHHNLPGPSGVGRPAGAQQRVAFASTPPPGGPTGMGAVAADAGPGPPDSGVPKKDSIVSLPELDSARSRLSSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLA2</accession>
    <name>A0A3B5LLA2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Circadian locomoter output cycles protein kaput</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLA2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011465"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011597.1">
        <property type="protein sequence ID" value="ENSXCOP00000011465.1"/>
        <property type="gene ID" value="ENSXCOG00000008650.1"/>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LM12</accession>
    <name>A0A3B5LM12_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Asparagine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Asparaginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Asn) + L-asparagine + ATP = L-asparaginyl-tRNA(Asn) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9659"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LM12"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008899"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009005.1">
        <property type="protein sequence ID" value="ENSXCOP00000008899.1"/>
        <property type="gene ID" value="ENSXCOG00000006789.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234618"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04323">
        <property type="entry name" value="AsnRS_cyto_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000151">
        <property type="entry name" value="Asparagine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000040">
        <property type="entry name" value="Asparagine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1910.20">
        <property type="entry name" value="asparaginyl-tRNA synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048952">
        <property type="entry name" value="AsnRS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF16">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20917">
        <property type="entry name" value="AsnRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="241"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="62"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="543" mass="62661" checksum="CF55CA9D4C2F22B5" modified="2018-12-05" version="1">MYVSDKHGSDQDGDGSEQKPFKTPLKALLFAGKEPFPTIYVESQKEGERWAVISKTQMKNAKKAFNREQMKNDAKDKKEAEDNERREKNLEEAKKIEIVNDPNLPEPETVKIHMLEAKRGERVKVFGWVHRLRRQGKNLMFIVLRDGTGFLQSVLSDKLCQCYNGLLLATESTVALYGTITVVPEGKQAPGGHELHCDFWELVGLAPAGGADNLLNEESDVDVQLNNRHMLIRGENVSKILRVRSTVTQCFRDHFFNRGYYEITPPTLVQTQVEGGSTLFSLDYFGEQAYLTQSSQLYLETCLPALGDTFCIAQSYRAEQSRTRRHLSEYTHIEAECPFISFEDLLTRLEDLVCDVVDRVLKSPAAQLLYDINPDFKPPKRPFKRMNYTEAIEWLREHDVKKDDGTYYEFGEDIPEAPERLMTDSINETILLCRFPAEIKSFYMQRCPEDRRLTESVDVLMPNVGEIVGGSMRIWDSDELLEGYKREGIDPTPYYWYTDQRKYGSCPHGGYGLGLERFLTWLLNRHHVRDVCLYPRFIQRCRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LN70</accession>
    <name>A0A3B5LN70_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Serine/threonine-protein kinase STK11</fullName>
            <ecNumber evidence="6">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">STK11</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LN70"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010911"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011036.1">
        <property type="protein sequence ID" value="ENSXCOP00000010911.1"/>
        <property type="gene ID" value="ENSXCOG00000008241.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158050"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042593">
        <property type="term" value="P:glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14119">
        <property type="entry name" value="STKc_LKB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000245">
        <property type="entry name" value="serine/threonine-protein kinase STK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000235">
        <property type="entry name" value="serine/threonine-protein kinase STK11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039154">
        <property type="entry name" value="LKB1_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF94">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="16 24 25" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="20" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18" id="KW-0464">Manganese</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 24 25" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 25" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="27">
        <location>
            <begin position="47"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="372"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="411"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="26">
        <location>
            <begin position="426"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00068788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="436" mass="49303" checksum="4CD395642786D8D3" modified="2018-12-05" version="1">MSDGDLPRLEYLNENELMEMDTFIHRIDSTEVIYQPRRKRAKLIGKYLMGDLLGEGSYGKVKEMLDSETLCRRAVKILKKKKLRRIPNGEANVKKEIQLLRRLQHKNVIQLVDVLYNEEKQKIYPLYCVCGMQEMLDSVPEKRFPVFQAHGYFCQLINGLEYLHSQGIVHKDIKPGNLLLTTDGSLKISDLGVAEALHPFAEDDTCRTSQGSPAFQPPEIANGLDTFSGFKVDIWSAGVTLYNITTGLYPFEGDNIYKLFENIGKGDYTIPEECGPLLSDLLRGMLEYDPAKRFSIQNIRQHNWVRKKHPPSERPVPIPASAESRDQWRSMTVVPYLEDLHGYTEDDDDELYDGEDEIIYTQDFTVPGQVAEEHQDQGNADHSPAVAKPVCVNGTDAGSLNSKTKAERRSSSSSNPSRKGVSTASKIRKLSTCKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPP9</accession>
    <name>A0A3B5LPP9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPP9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014083"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014258.1">
        <property type="protein sequence ID" value="ENSXCOP00000014083.1"/>
        <property type="gene ID" value="ENSXCOG00000010666.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063983"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057810">
        <property type="entry name" value="RBD_ZCCHC3_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057811">
        <property type="entry name" value="RBD_ZCCHC3_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042509">
        <property type="entry name" value="ZCCHC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639">
        <property type="entry name" value="GAG-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22639:SF3">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23057">
        <property type="entry name" value="RBD_ZCCHC3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23058">
        <property type="entry name" value="RBD_ZCCHC3_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="208"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="227"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="271"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="278"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="380" mass="43037" checksum="0961C4DAD6D28A93" modified="2018-12-05" version="1">MPRNLNTGTLSTQVPLMVRGWMSVLFLLKSITSSFVLEVRLILQKELGISPAQMDYIFAMPGRRIFEVIFTTNTFFEKCLGSFGRLKETRPQLANIEMTSLSQTEPKSITVLMFSEQVRMEDIRTWLQQRCTVKHGYELKDEDGVRTGGRRFFVQLKRNLATGETYHLPPVIQLGAIRGHVFYPGQPKTCRRCGSQQHLSAECRITYCKNCGTEGHLTKDCQEPVNCNLCGERGHTFKSCPKSYANRVKTFASQETSGRADLGDQLLQMETNTEPDEEKQLESAEEQRKENGGTTPMIPPRSPRLQEGIVSQCYLTRGAAFHWLMPILPGHVRGRLTNTLSFPLAKYSIMAELIQLLHLEACLLHSLTLAKQINMQCVCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQC4</accession>
    <name>A0A3B5LQC4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the importin beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQC4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009979"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010097.1">
        <property type="protein sequence ID" value="ENSXCOP00000009979.1"/>
        <property type="gene ID" value="ENSXCOG00000007570.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063347"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051345">
        <property type="entry name" value="Importin_beta-like_NTR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040709">
        <property type="entry name" value="Importin_rep_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040944">
        <property type="entry name" value="Importin_rep_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057941">
        <property type="entry name" value="TPR_TNPO3_IPO13_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057942">
        <property type="entry name" value="TPR_TNPO3_IPO13_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058537">
        <property type="entry name" value="TPR_TNPO3_IPO13_4th"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363:SF33">
        <property type="entry name" value="IMPORTIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12363">
        <property type="entry name" value="TRANSPORTIN 3 AND IMPORTIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18773">
        <property type="entry name" value="Importin_rep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18786">
        <property type="entry name" value="Importin_rep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24138">
        <property type="entry name" value="TPR_TNPO3_IPO13_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24140">
        <property type="entry name" value="TPR_TNPO3_IPO13_3rd"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF24139">
        <property type="entry name" value="TPR_TNPO3_IPO13_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="532" mass="59707" checksum="00E04FA50BF9145C" modified="2018-12-05" version="1">METTHGICRIAVALGETHSRVLLGQPDHWQEYLALVNMILFCTGIPGHYPVNETTSSLTLTFWYTLQDDILSFEEEKQAVYLQVYKPVYFQLVDVLLLKSHYPSQEEYASWSSDDKEQFRIYRVDISDTLMYVYEMLGAELLSNLYDRLGRLLMDPQQSAVWQDIEALLFGFQSIAETIDVNYSDVIPGLIGLIPRINISNVMLADTVMYTIGSLAEWLADHPVMLGGILPMVLRGLVKAELSISSVSTLKRICRECRQDLAPYAQDILTVSQDVLVKEIHESSQCMWLMQALGFLLSALPAEEILGRLHSLISPHIQQLDSLVRQEPDPATKQGIMSILGLLSSLFTTLDVNRHADGLDEEDASPRLTAAQSIQNPVVVVLQQVFPLIQTIISKWLHDSEVVEAVCGVFDKTVRTLLHDFGPMVARLSEMLGQIYSTYPQAVGGGSSRSLAENFSEVLLSLNRHSPGLLSQWLTETLQTPGFPSAHVTMEQKHTFSQQILREQTNKRRVKETVKEFSLLCRGQQGAGFSEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQP3</accession>
    <name>A0A3B5LQP3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQP3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010218.1">
        <property type="protein sequence ID" value="ENSXCOP00000010099.1"/>
        <property type="gene ID" value="ENSXCOG00000007662.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182802"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="50" mass="5256" checksum="253B924494BD7460" modified="2018-12-05" version="1">MGKAKKPKAAPVETKKKAAPKKAPKAKKAKPAENGNTKAEEPKAEATETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRV9</accession>
    <name>A0A3B5LRV9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Teneurin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRV9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014808"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014994.1">
        <property type="protein sequence ID" value="ENSXCOP00000014808.1"/>
        <property type="gene ID" value="ENSXCOG00000011226.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234566"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009471">
        <property type="entry name" value="Ten_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06484">
        <property type="entry name" value="Ten_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51361">
        <property type="entry name" value="TENEURIN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Teneurin N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="165" mass="18601" checksum="80F6C02558607436" modified="2018-12-05" version="1">MEEMDSKPYQPLSKGRHEMEMSYTSSSDESEDGRTHHRSYTSRETLPDYTHELRLTLGSHRDRQSNYSQPQPVFRDQTLCTGPAAHVHSRGYSLGVGSDVDTEPEVDPSPAHGLQHMWMRGLKSEQSSCLTSRANSALSLTDTEHDRKSEPDNGETNAARHTCRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSE1</accession>
    <name>A0A3B5LSE1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SUN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus inner membrane</location>
            <topology evidence="5">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSE1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013740"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013908.1">
        <property type="protein sequence ID" value="ENSXCOP00000013740.1"/>
        <property type="gene ID" value="ENSXCOG00000010394.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155830"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034993">
        <property type="term" value="C:meiotic nuclear membrane microtubule tethering complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000009">
        <property type="entry name" value="SUN domain-containing protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045119">
        <property type="entry name" value="SUN1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911">
        <property type="entry name" value="SAD1/UNC-84-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911:SF23">
        <property type="entry name" value="SUN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="SUN" evidence="9">
        <location>
            <begin position="623"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="404"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="786" mass="88506" checksum="FB2A5D4421B02A45" modified="2018-12-05" version="1">MDFSQLHTYTPPQCAPENTGYTYSLSSSYSTAALEFEKEHQISPVYESPKMLRRSLRLQNSSSHYNTENVTDYSLDHGSSYIDTRKETRNVECSVSLPQESYSLQWKSYFENQIWLNLLPGCHVPEQHSSQSSSNTNGEASFSKSKTSVPNGYICKDCSFHSQQSDSRVTRSSLTSLSSSSSSCQEAELSTDGHSSFCGSMNVKDLVTEETSHLNLNGSLLKAGKGLMWFLARGWYQLVSLMSLVNVFFLTRIFSLTSLFTFNIAFFCSPAVFWWWLPFTGALLTYLPAINLTEWRSTSRFDFFSNLAAVPAAVPATETPMEQAHNTPVSQATRQLAVLWEKVQAGNQKQDQRHSNVLGLYSSLREQVHSQNERESFEVWVSSMMEQRLGVLRGELQRENLNIAQSSEQQKQQQESQAARLANLELLLRALETKTEVFSVLFISVGVKQEDHDALLLEMQRLEAELSKIRGDLQGFAGCKGKCEQLNTLQQTVSSINVPELKNLFFGSDESADQQQGEIPESLIQWLSQRYVSMPNLQAALASIEMSILKNISLQLEHSRAQVLGEAESRARSVFETVRGTVRHTSTTEGLSEEDVNLIVQNALKLYSQDRTGQVDYALESGGGSILSTRCSETYETKTALMSLFGLPLWYFSQSPRVVIQPDVYPGNCWAFKGSQGYLVIRLSLRIRPTSFCVEHIPKTLSPTGNIASAPRNFTVFGLDDEYQEEGTLLGHYTYEEDGDSLQIFPVKEKNDKAFQIVEVRVLSNWGHPEYTCLYRFRVHGEPLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTP3</accession>
    <name>A0A3B5LTP3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription factor E2-alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Transcription factor 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTP3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012696"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012850.1">
        <property type="protein sequence ID" value="ENSXCOP00000012696.1"/>
        <property type="gene ID" value="ENSXCOG00000009590.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157036"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18945">
        <property type="entry name" value="bHLH_E-protein_TCF4_E2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000001">
        <property type="entry name" value="Putative transcription factor 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051098">
        <property type="entry name" value="NeuroDiff_E-box_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11793">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11793:SF7">
        <property type="entry name" value="TRANSCRIPTION FACTOR E2-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="9">
        <location>
            <begin position="508"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="98"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="276"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="382"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="591"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="276"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="453"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="487"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="499"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="614" mass="66137" checksum="88C032C43A5F3C1B" modified="2018-12-05" version="1">MSEQQQRMAAVETDKELSDLLDFSAMFAPPVANGKNRTMTLASTHFSGLEPELYSVTLILQSYGEGSHFSEHEGLSSPFISSGIPGLFPSDIAMPSPDALSPSGIKSGSQFYPSYPNNPRRRPPEGGLETQPKKIRKPPGLPSSVYASTSGDEFARDNGGYPGAKPGAVYPGTFYMQEDPWSSSGYSGMLGNSPHIGQPGSFPAINPQDRMLHSLSLIQQNYSLPGSEVNGFHSAPTTYNHTSTINGEGIMANRGTTASSSGDEIGKALASIYPSDHNSNNFSSAPSTPGSPQAIPGAQSQWQRPTTPNYEGQPHTLQNKMEDRLEEAIHVLRSHAVGQSPALEGAHSDMHSLLSSVHNGGLGGLSPAFPNASLALSNRHPALQGGKHEEPTGLPPSSTLLHGHHATGPTPSVGQPEGFTSLPGGLARSAHSSSSSDIKREDKEDDENSSIADKSDEEKKDSKAARNRTRKEALTLQMLSGLSDQKDDQDNEDDEDVPPEVKMERERERRVANNARERLRVRDINEAFKELGRMCQLHLSHDKPQTKLLILHQAVNVILNLEQQVRERNLNPKAACLKRREEEKVSGVVGEAPMQLPGGHPSMGGDGHNPVGHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUL0</accession>
    <name>A0A3B5LUL0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Galanin receptor 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027871930.1">
        <property type="nucleotide sequence ID" value="XM_028016129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUL0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012996"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013156.1">
        <property type="protein sequence ID" value="ENSXCOP00000012996.1"/>
        <property type="gene ID" value="ENSXCOG00000009848.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114143800"/>
    <dbReference type="KEGG" id="xco:114143800"/>
    <dbReference type="CTD" id="561989"/>
    <dbReference type="GeneTree" id="ENSGT01150000286969"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004966">
        <property type="term" value="F:galanin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000405">
        <property type="entry name" value="Galanin_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695:SF25">
        <property type="entry name" value="GALANIN RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00663">
        <property type="entry name" value="GALANINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="26"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="277"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="42"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="344" mass="38182" checksum="562EBCBDC79F3F66" modified="2018-12-05" version="1">MVPPGNESYSWAETNRTAGEPGPEVVIVPLVFGLIFVVGLLGNSLVMLVIGRVKPSGGARPLSPTNIFIVNLSVADLLFLLFCVPLHATIYSLPEWVFGAFLCKFGHFFTTASMLVSILTLVAMSVDRYIAVVRSKSSACVRSRRNALAGLCVIWTLSLLCSVPVAQNYVLTSHSSAPNKTFCWEAGLGAPRRAYKVSILLLGFILPLLLICCCYIRVLCHLHKKMKNMSKRSKLSKRKTVQTILLVVTGFTICWMPHHIIVMWVEFGTYPLNDATFALRIVSHCLSYGNSCINPILYAFLSENFRKACRQVFSRHLLYAPPADGRVVQFRMDNFSTTHSSTNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUZ5</accession>
    <name>A0A3B5LUZ5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Calcium/calmodulin-dependent protein kinase Ia</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUZ5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011654"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011787.1">
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        <property type="gene ID" value="ENSXCOG00000008800.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
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        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
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    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
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    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="313" mass="35605" checksum="7F4651DF57107580" modified="2025-10-08" version="2">MQDVTEGAFSEVVLAEEKRTQRLVAIKCIPKKALEGKENNIENEIAVLHRIKHPNIVSLEDIFESMSHLYLVMQLVSGGELFDRIVEKGFYTERDASQLIHQILDAVKYLHDMGIVHRDLKPENLLYYSMDEDSKIMISDFGLSKIEGAGSVMSTACGTPGYVAPEVLAQKPYSKAVDFDLLLCGYPPFYDENDAKLFEQILKAEYEFDSPYWDDISDSAKDFICHMMEKDPMKRYTCEQALQHPWICGDTALDKNIHESVSAQIKKNFAKSKWKVSQQTVKGADLGPAGVGLHVFFFACLSHLDIYIYIFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVR5</accession>
    <name>A0A3B5LVR5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="Ensembl" id="ENSXCOT00000015148.1">
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    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13733">
        <property type="entry name" value="SPRY_PRY_C-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="21"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="8">
        <location>
            <begin position="177"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="9">
        <location>
            <begin position="365"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="90"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="138"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="564" mass="63062" checksum="BF1FB01F13FAC960" modified="2018-12-05" version="1">MSVQAAMALPSAFLSEEQFTCSICLEMFKNPVSTPCGHSFCQSCLSTYWDGRGRGQAAKAYQCPLCKESFSNRPELQINRTLREITEQFKQMSQSDGGAGGGGEHLGLRAQQSGPSPPARPGEMPERLLVEMMARFQGLTPPSPHQGFQTQNNHDPPPPYSPSLRRGAAQSLPDVQPNLPLCPIHMRGMEFFCRTDNMCVCSVCVEMADHRGHSVAPAQREWHIKKSQLGIHEMELKELICMRERKVEEIRNSIRKIKVNMSAGYRTACFYYFDSSHQVIEMTVTTAESRARSLLKELEEEIAALKKRSATLGQLAVSEDYVLFFKTFPALSSPPQAKDWSNVSVSSQSTSEGILRTISQMVERLQLDLQNLPEWEIKQQPLGEDITLDPSTAHPRLIISADGKQVQCGDRHQPVPDNPERFDRVVCVMAHQGFTSGRHYWEVDVSSKTDWDLGVASRSVIRKGKIPVSPAHGYWFLSLRDRTDYAFRTDPATSLTVATRPSRVGVYIDCDKGLLSFYNVEAKLVIFTYTDSFKDTIHPFFSPCTNKSGQNAAPLVICPTAVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWB3</accession>
    <name>A0A3B5LWB3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nuclear prelamin A recognition factor-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NARF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWB3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013611"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013778.1">
        <property type="protein sequence ID" value="ENSXCOP00000013611.1"/>
        <property type="gene ID" value="ENSXCOG00000010308.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153514"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045765">
        <property type="term" value="P:regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002040">
        <property type="term" value="P:sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.950.10">
        <property type="entry name" value="Fe-only Hydrogenase (Larger Subunit), Chain L, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050340">
        <property type="entry name" value="Cytosolic_Fe-S_CAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009016">
        <property type="entry name" value="Fe_hydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004108">
        <property type="entry name" value="Fe_hydrogenase_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003149">
        <property type="entry name" value="Fe_hydrogenase_ssu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11615">
        <property type="entry name" value="NITRATE, FORMATE, IRON DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02906">
        <property type="entry name" value="Fe_hyd_lg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02256">
        <property type="entry name" value="Fe_hyd_SSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00902">
        <property type="entry name" value="Fe_hyd_SSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53920">
        <property type="entry name" value="Fe-only hydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iron hydrogenase small subunit" evidence="2">
        <location>
            <begin position="408"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="474" mass="53016" checksum="BACA2A5364CB6850" modified="2018-12-05" version="1">MASHFSGVLQLTDLDDFITPSQECVKPVKVEKKKGKSVAKIQIEDDGSYVQVNQDGGKQKLERAKITLNDCLACSGCITSAESVLITQQSHEELLKVLRNNKTKETEQKTVVVSVSPQSRASLAAHYNLSSSEAGRKLTSFLKGLGVHHVFDTSFSRTFSLLESQREFVDRFSRKQQDSKAMPMLTSACPGWICYAEKTHGDYILPYISTTRSPQQMMGSLVKSYFAEQQGLSPQQIYHVAVMPCFDKKLEASRSDFYLSKAETREVDCVITSGEVQKMLEEQNVSLSDVEPAALDTISVCGDEFLSHAGSGSGGYLQHVFTYAAKQLFGEEVKELTYKTLRNKDFQEVRLEKDGAVLLCFASTYGFRNIQNLVQKLKRGKSPYHFVEVMACPSGCLNGGGQVRPSSSQNQKELLQQVEELYKAERPLLPENDTRVAELYESWLHTLGAERAKELLHTQFHAVEKMTNGLTIKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWR7</accession>
    <name>A0A3B5LWR7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">P2X purinoreceptor 7 intracellular domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWR7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015582"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015775.1">
        <property type="protein sequence ID" value="ENSXCOP00000015582.1"/>
        <property type="gene ID" value="ENSXCOG00000011784.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287036"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001614">
        <property type="term" value="F:purinergic nucleotide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046815">
        <property type="entry name" value="P2RX7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003050">
        <property type="entry name" value="P2X7_purinoceptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36981:SF3">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE FAMILY PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36981">
        <property type="entry name" value="ZGC:195170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20478">
        <property type="entry name" value="P2RX7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01314">
        <property type="entry name" value="P2X7RECEPTOR"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="P2X purinoreceptor 7 intracellular" evidence="1">
        <location>
            <begin position="71"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="211" mass="23331" checksum="5A97384258ECC1DC" modified="2025-10-08" version="2">MVLSCFSKKRFSFSTSVEPEQRNELCSGSLWLTEPCSTGKLFMLRYVQESVPTALCLLQPDVGADAGPPQDQNLTPDPKRRQPGWCKCGCCTSSALPQEELCCRRSGGACIASSPPFRRLVLDRSLLQAVLLYQDPLAPPAGGAQPAALRHCAYRQFISWRFGLPPDDSHPVIPRCCVRRIREEYPSPDGRYSGFTPIRTVSVPTQNNGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWW5</accession>
    <name>A0A3B5LWW5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Copine family member IX</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the copine family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWW5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015380"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015572.1">
        <property type="protein sequence ID" value="ENSXCOP00000015380.1"/>
        <property type="gene ID" value="ENSXCOG00000011643.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159659"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF112">
        <property type="entry name" value="COPINE-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="3"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="174"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="514" mass="57630" checksum="464162EA47732BD6" modified="2018-12-05" version="1">MFQPGTVLVSNHTVCLLQGVLLEQSPLPHSALWQVLYDAVSFQFPLLHSAVLYVLTAPSTPLTLISQTFPCQFNSSVFCKFGRTEVIDNTRNPDFVRKFVLDFFFEEKQNLRFDVYNVDSRSCNISKHDFLGQTFCTLGEIIGSTGGRLERTLSIFSATSLTGACMCAGIPGKKCGVIILAAEELSNCRDIATMQLCANKLDKKDFFGKSDPFLVFYRSNEDGTFTICHKTEVIKNTLNPVWQSFTIPVRALCNGDYDRSGILAPHDFIGEFTTSYRELSRGQNQFNVYEVKNMCFCLFSFYSALFVYFDPFFYLVAFDGTLSNLLNYFCFPSYVCACVSILFFSVLQNGDSDQPNCVGIEGVLEAYFQSLRTVQLYGPTNFAPVINKVANCAAEITDGSQYFVLLMITDGVISDMVQTKEAVVNAALLPLSIIIVGVGPAEFDAMEELDGDEVRVSSRGRLAERDIVQVLLMIIFKFAGLNIRLSYYKLSVCRIYFDVFLSMPVHFHTLNTFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYZ5</accession>
    <name>A0A3B5LYZ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Perilipin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the perilipin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027884363.1">
        <property type="nucleotide sequence ID" value="XM_028028562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYZ5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012999"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013159.1">
        <property type="protein sequence ID" value="ENSXCOP00000012999.1"/>
        <property type="gene ID" value="ENSXCOG00000009844.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114151390"/>
    <dbReference type="KEGG" id="xco:114151390"/>
    <dbReference type="CTD" id="10226"/>
    <dbReference type="GeneTree" id="ENSGT00950000182920"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010890">
        <property type="term" value="P:positive regulation of triglyceride storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.340">
        <property type="entry name" value="Flagellar protein FliS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.170">
        <property type="entry name" value="Perilipin, alpha-beta domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004279">
        <property type="entry name" value="Perilipin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024">
        <property type="entry name" value="PERILIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14024:SF11">
        <property type="entry name" value="PERILIPIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03036">
        <property type="entry name" value="Perilipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036881">
        <property type="entry name" value="PAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109775">
        <property type="entry name" value="Mannose-6-phosphate receptor binding protein 1 (Tip47), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="300"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="426" mass="45072" checksum="80DE29CE40AFD148" modified="2018-12-05" version="1">MADSGKTNEAGAAATEPANGEQQNVVARVSHLPLVSSACEAVSNAYSSTKDNVPLLKGVMDVAESGVRTLGAAASTGSKPVLGMIEPQLATVNEYALKGLNKMEETLPILHQPADKVVSDTVGMVYQSVTGAKDAVLGAVMSGVELTRAAVSGSINTVMSTRMGQMVSSGMGLALSRSEDWVDQNLPLTERELAAVAEPANSEVSTTSANPSYFVRLGKLSAKVQERALEQSLGRARQARDATYSAVAQITSTLDLVESARASLGTAGNQIAGAPEQLLQRWTEWKKKREGEGLTESEPDGSKDESEQLESRALSMVRGLSDQLRASCSSVVSSAQGLPGTVQEQLTSAQVAAEELQTSLRNASTLTPVLLERSRHHLKQVQHSLDGVMEFLLHNTPLNWLVGPFGAQKTESEQGEVQMEQASSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M066</accession>
    <name>A0A3B5M066_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="12">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PPP phosphatase family. PP-2B subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M066"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016650"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016865.1">
        <property type="protein sequence ID" value="ENSXCOP00000016650.1"/>
        <property type="gene ID" value="ENSXCOG00000012557.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165110"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033192">
        <property type="term" value="F:calmodulin-dependent protein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097720">
        <property type="term" value="P:calcineurin-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07416">
        <property type="entry name" value="MPP_PP2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041751">
        <property type="entry name" value="MPP_PP2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043360">
        <property type="entry name" value="PP2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45673">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2B CATALYTIC SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="14">
        <location>
            <begin position="117"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="456"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="476" mass="54046" checksum="7E6327F7BE5212BE" modified="2018-12-05" version="1">MKEVFDSEGRPRVDLLKAHLTKEGRLEEAVALRIIDEGAAILRQERTMLDIEAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWSLKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSEQVYDSCMEAFDCLPLAALMNQQFLCVHGGLSPEIHTLDDIKKLDRFKEPPAFGPMCDLLWSDPLEDFGNEKTQEYFGHNTVRGCSYFYSYPAVCEFLQTNNLLSIIRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLSICSDDELVNDGEEIYDASAAAARKEVIKNKIRAIGKMAKMFSVLREESENVLTLKGLTPTGMLPSGVLSGGKQTLQSAIRGFSPQHRISSFEEAKGLDRINERMPPRRDAVNSVGLSMGRMNMGEPNGTDNNSNIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0Z6</accession>
    <name>A0A3B5M0Z6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0Z6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017992.1">
        <property type="protein sequence ID" value="ENSXCOP00000017768.1"/>
        <property type="gene ID" value="ENSXCOG00000013382.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156009"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.640.10:FF:000010">
        <property type="entry name" value="Thyroid peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.640.10">
        <property type="entry name" value="Haem peroxidase domain superfamily, animal type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019791">
        <property type="entry name" value="Haem_peroxidase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037120">
        <property type="entry name" value="Haem_peroxidase_sf_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475">
        <property type="entry name" value="OXIDASE/PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475:SF60">
        <property type="entry name" value="THYROID PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03098">
        <property type="entry name" value="An_peroxidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00457">
        <property type="entry name" value="ANPEROXIDASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50292">
        <property type="entry name" value="PEROXIDASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0768">Sushi</keyword>
    <feature type="domain" description="Sushi" evidence="5">
        <location>
            <begin position="561"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="614"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="618"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="2">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR619791-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="644" mass="71333" checksum="C11B312440B9B870" modified="2018-12-05" version="1">VSELLSRENLELLAELSQCPTETDPAVCEGSHHDKYRSISGVCNNRQNPDWGAANTALVRWLPAEYEDGEEEPRGWDPQRLHHGSQLPPPRRVSREVVRTSCKGSDAAYSQLLADWGQYIDHDVTLTPQSLSGAASWAQRDCRTSCGNLHPCFPIQDSLCMPFHRSTPACSSRTGSGILRALQRQQLNAVSSFIDASLVYGHTPQLQRDLRDLSGRSGKLAVNGRFRDRQGRPYLPFVAATPSACGRGMECFRAGDGRVNEGLPLIALHTLWLREHNRLAETLKLINQHWSPEAVFQEARKIVGALHQVRVRRPLQDRCSSHTNITSLMPLNKNLGLICFCVYRSNECGVCVCAGGIEPTLRGMVATAAAVTAPDSLLVEEVTESLLVLDSQQNLDLAALNLQRGRDHGLPGYNDWRDFCGLKRIATLDDLAEVVRDRRVAEKILHLYQHPDNIDVWLGGLVENFLPGSRTGPLFACLIGKQMKLLRDGDRFWWEADGVFTRQQREQLWKTSLSRIICENSDIQEIPADPFRLAQYPAGFLHCSAVPALSLEAWRDEPSLRLCGSPRPVDNGDFLFSSRSGKLTAQYACFHGFQLEGATEAVCEGGVWSADPPRCQRSAPGTSSPSPCSAPPSCRTLRVSPSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2F5</accession>
    <name>A0A3B5M2F5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">rhomboid protease</fullName>
            <ecNumber evidence="4">3.4.21.105</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M2F5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017592"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017815.1">
        <property type="protein sequence ID" value="ENSXCOP00000017592.1"/>
        <property type="gene ID" value="ENSXCOG00000013269.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158838"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000279">
        <property type="entry name" value="Rhomboid, veinlet-like 3 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000002">
        <property type="entry name" value="Rhomboid, veinlet-like 3 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051739">
        <property type="entry name" value="Rhomboid_IM_Serine_Proteases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840:SF5">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="247"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="338"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="373"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="403"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="103"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="437" mass="49288" checksum="9A8CC1041B3F137A" modified="2018-12-05" version="1">MIPDFIFREVVFLAYSPVCVGAAAFLQALFANVNSLKNLPDPRNLPSLFFHNIQLYENEAEEKKPSLIIWPHLCLPLQYDPENSGFISTERFRDLLATHGSELDPHKLEVLLALTDGNSDGKICYQDFVNLMSNKRSNSFRRAILQGSRQLKGCSLRDEVGLGLSQRLVRHVAYETLPREVDRKWYFDSYTYCPPPWFILAITIAEVAVFMYYGIQLDRLVLQVSSPSFLKSPLPYHPQLRAQAWRYFSYVFMHTGIEHLSLNMAMQLLVGVPLEMVHGALRIGLIYVCGALAGSLAVSVTDMTAPVVGSSGGVYALVSAHLANVVMNWSGMKCQFKLFRMAMALVCMSVEFGRAVWLRFYPPAFPPCPNPSFVAHLGGVLVGLTLGVVVLQNYEQRLQEQSLFWIFFCVYTLFVLCAVFWNVFAYSLLDVRIPPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M510</accession>
    <name>A0A3B5M510_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UDP-glucuronosyltransferase</fullName>
            <ecNumber evidence="8">2.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor beta-D-glucuronoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132367"/>
            <dbReference type="ChEBI" id="CHEBI:132368"/>
            <dbReference type="EC" id="2.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.17" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M510"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018760.1">
        <property type="protein sequence ID" value="ENSXCOP00000018527.1"/>
        <property type="gene ID" value="ENSXCOG00000013945.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164678"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000001">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000203">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF52">
        <property type="entry name" value="UDP GLUCURONOSYLTRANSFERASE 5 FAMILY POLYPEPTIDE B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017101838" description="UDP-glucuronosyltransferase" evidence="8">
        <location>
            <begin position="19"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="480"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="524" mass="60287" checksum="7AD7053158614C9C" modified="2018-12-05" version="1">MHWIWISLIWLCPVVVHSGKVLVLPVDGSHWVNMKVIIEELHSRGHQIFVVRQTDSWYIKESSPFYTSINTDIESGFDEDFIKSFVTELLKIQREGKSVWTRIKLEMEQAQKISEFHIKSAKGLEKTFESKELIQSMQDENYDLVLTDPALPMGVILAHYLKLPLVFNVRWTSHGEGHFSIAPSPLSYVPMTGTEMSDKMSFIQRVLNVMIFALNEYQIMWHVLPYYEALFAKYFGSDFDYLSMFQTADLWLMRVDFVFEFPRPTMPNVIYMGGFQCKPAKHLPQDLEEYVQSSGEHGVIIMSLGTLIGDLPHDLAEVIAGAFAKLPQKVIWRYKGVRPANLGNNTLVVDWMPQNDLLGHSKIKLFVAHGGTNGIQEAIYHGVPILGLPLIFDQPDNLLRIEVRGAGKNIQISTMTEDTFLQGIQEVLTEPSYSINMQRLSRLHRDQPMKPLDQAIWWIEFVMRNRGAAHLRTESYRLPWYSYHCVDVMLCLGGAVLVIFGVFAVLLKCLWNFSRKKTQREVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5G4</accession>
    <name>A0A3B5M5G4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NF-kappa-B inhibitor alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">I-kappa-B-alpha</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals. On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NF-kappa-B inhibitor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M5G4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018615"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018849.1">
        <property type="protein sequence ID" value="ENSXCOP00000018615.1"/>
        <property type="gene ID" value="ENSXCOG00000014008.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163080"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051059">
        <property type="term" value="F:NF-kappaB binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071356">
        <property type="term" value="P:cellular response to tumor necrosis factor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034142">
        <property type="term" value="P:toll-like receptor 4 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051070">
        <property type="entry name" value="NF-kappa-B_inhibitor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46680">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46680:SF1">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="116"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="188"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="222"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="14"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="291"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00041123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="323" mass="36057" checksum="6CA2CC40421DF011" modified="2018-12-05" version="1">MDLHRASILNQMDYNRESKEGKAAQSTEERLDSGVDSLKEEEYQAVAEELHRLQLESERQQAAARTEEPLQEWQTQTTDEGDTLLHLAIIHEAKDYIKQMIDLSKNTPFLNAQNDLRQTPLHLAVITNQPNVCSSLLASGCDPTLVDNSGDTPLHIACRHGNLYCFSVITQNCRLEHLHTAMAACNYNGQNCLHLASVHGFLSLVEKMVDLGADINAKEQHNGRSSLHLAVDQQNLALVKLLLRKGANPNLLSSGGHTPFHLTYGLDNCDIQKELFPVTHPDLRDLSESENSGEESSSEESDEEVVKILTGWGMYDDIQWNGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M662</accession>
    <name>A0A3B5M662_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carbohydrate sulfotransferase</fullName>
            <ecNumber evidence="11">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sulfotransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M662"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015399"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015591.1">
        <property type="protein sequence ID" value="ENSXCOP00000015399.1"/>
        <property type="gene ID" value="ENSXCOG00000011661.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161154"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030166">
        <property type="term" value="P:proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018011">
        <property type="entry name" value="Carb_sulfotrans_8-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137:SF60">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="307" mass="35966" checksum="36327A7A19B13411" modified="2018-12-05" version="1">MFFSKTSSRPGKSRRSPLQTLYKGDQLKLSAAQKSLQGRRKVLEQACLSHTKKRRVLSPEDLKHLIVDDKHGLIYCYVPKVACTNWKRVLMVLSSNGRYSDPLAIPANEAHVAGNLRTLSDFSVTEINQRLRSYLKFIFVREPFERLVSAYRNKFTRNYNTAFHKRYGTKIIRRHRANPQPEALEKGNDVSFREFIQYLVDPQTQREEPFNEHWERVHSLCHPCLIHYDVVGKYETLEPDAHAVLRLAGVEGKVQFPTSSKSTRTDGNMAARFFKHISPFYQKKLFNLYRMDFLLFNYTTPEYLRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6T1</accession>
    <name>A0A3B5M6T1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6T1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020200.1">
        <property type="protein sequence ID" value="ENSXCOP00000019953.1"/>
        <property type="gene ID" value="ENSXCOG00000014984.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271948"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034113">
        <property type="term" value="P:heterotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150079">
        <property type="term" value="P:negative regulation of neuroinflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047164">
        <property type="entry name" value="OX2G-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46841:SF7">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46841">
        <property type="entry name" value="OX-2 MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="39"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="290" mass="32651" checksum="4D001B51D5592156" modified="2018-12-05" version="1">MLFLWTSIVSPAIISFYRITCMQLTFHLTFQVGHSALFPALIIHVVETQHTRIAQVGEDAQLSCWFLGSNGVQEVIWEKLSGSTMHGVGSYNLLSGAKVFPPFKDKVQFAYSGLKNSSIIVKNVTEQDVGCYLCLFTSYPFRNLTAETWSLVTCSATGQPAPTVTLTVSHMVYYSHYNPSSKTNTNGTITITTTALLSAVNSTLVRCSVEVDKTVEKSKKVLNIMRCLRGKLMLIQLKTKIIKYSSILYWCRIKIVKPVKNVKRNSCVLLLAFKYKRSEKKRVFELKHTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6W1</accession>
    <name>A0A3B5M6W1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Neurexin 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Neuronal cell surface protein that may be involved in cell recognition and cell adhesion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6W1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015544"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015737.1">
        <property type="protein sequence ID" value="ENSXCOP00000015544.1"/>
        <property type="gene ID" value="ENSXCOG00000011722.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154292"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000015">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000029">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000004">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000005">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF51">
        <property type="entry name" value="NEUREXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 10" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Laminin G" evidence="11">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="11">
        <location>
            <begin position="179"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="376"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="11">
        <location>
            <begin position="418"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="11">
        <location>
            <begin position="596"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="774"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="915" mass="101267" checksum="2B927E04FC144206" modified="2018-12-05" version="1">YDLSLNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLALKNGAVSLVINLGSGAFEALVEPVNGKFNDNEWHDVKVTRNLRQVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRLELSRLAKQDDPKMKIHGTVAFKCENVATLDPITFETPESYIILNKWNAKKTGSISFDFRTTEPNGLLLFSHGKSKQQPKDSKTPQTLKVDFFAIEMLDGHLYLLLDMGSGTTKTKAVNKKVNDGEWYHVDFQRDGRSGTISINTLRTAYTAPGESEILDLDDNLYLGGLPENKMGLVFPTEVWTALLNYGYVGCIRDLFIDGQSKDVRRLAEIQKAAGVKPSCSKEPPKQCLSNPCQNNGVCREGWNRYVCDCSGTGYLGRSCEREATILSYDGSKFMKVQLPVVMRTEAEDVSLRFRSQRAYGLLIATTSRESADALRLELESGRVRLTVNLGKGPETIFAGQNLNDNEWHTVRVFRRGKSLKLTVDELPPVEGQMAGDHTQLEFHNIETGIVTEKRFMSMVPSNFIGHLQSLSFNGMAYIDLCKNGDIDYCELNAMIGFKNIIADPVTFKSRSSYVTLTTLQAYYSMHLFFQFKTTSPDGLILYNSGDGNDFIVVELVKGYLHYVSDLGNGAHLIKGNSNKPLSDNQWHNVIISRDTNNLHTVKIDTKVTTQTTSGAKNLDLKGNLYIGGVAKEMYKDLPKLVHAKEGFQGCLASVDLNGRLPDLMSDALDCVGQIERGCEGPSTTCQEDSCANQGVCLQQWEGFSCDCSMTTFGGPLCNDAGTTYIFGRDGGLITYTWPPNDRPSTRADRLAIGFSTHLKDAVLVRVDSSSGLGDFLKLHIVSQNFIHSDALRCKITFSFSAGSKCWYIYPWSGQDPKSRLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7C9</accession>
    <name>A0A3B5M7C9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7C9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020168"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020415.1">
        <property type="protein sequence ID" value="ENSXCOP00000020168.1"/>
        <property type="gene ID" value="ENSXCOG00000015115.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032433">
        <property type="term" value="C:filopodium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032426">
        <property type="term" value="C:stereocilium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051491">
        <property type="term" value="P:positive regulation of filopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030832">
        <property type="term" value="P:regulation of actin filament length"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052409">
        <property type="entry name" value="Myosin-III_kinase_activity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46256">
        <property type="entry name" value="AGAP011099-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46256:SF4">
        <property type="entry name" value="MYOSIN-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-0518">Myosin</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myosin motor" evidence="11">
        <location>
            <begin position="51"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="105" mass="12079" checksum="7EAE74C720CEE0FC" modified="2018-12-05" version="1">MVLPHCWCLKARFHSEAQAGVQKICFSVGFPQSQNVLRPGLVALSLGAGSNTVTEHLQSRYTRDQIYTYVGDILIAVNPFHDMEIYSPQRTRWEQHRCHIRLQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7L4</accession>
    <name>A0A3B5M7L4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dual specificity protein phosphatase</fullName>
            <ecNumber evidence="10">3.1.3.16</ecNumber>
            <ecNumber evidence="10">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Dual specificity protein phosphatase; active with phosphotyrosine, phosphoserine and phosphothreonine residues. The highest relative activity is toward ERK1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 18">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="10"/>
    <dbReference type="EC" id="3.1.3.48" evidence="10"/>
    <dbReference type="RefSeq" id="XP_027874581.1">
        <property type="nucleotide sequence ID" value="XM_028018780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7L4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019480"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019720.1">
        <property type="protein sequence ID" value="ENSXCOP00000019480.1"/>
        <property type="gene ID" value="ENSXCOG00000014631.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114145292"/>
    <dbReference type="KEGG" id="xco:114145292"/>
    <dbReference type="CTD" id="1847"/>
    <dbReference type="GeneTree" id="ENSGT00940000159529"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001706">
        <property type="term" value="P:endoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01446">
        <property type="entry name" value="DSP_MapKP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000057">
        <property type="entry name" value="Dual specificity phosphatase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000023">
        <property type="entry name" value="Dual specificity protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008343">
        <property type="entry name" value="MKP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF40">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000939">
        <property type="entry name" value="MAPK_Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01764">
        <property type="entry name" value="MAPKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4 10" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="15">
        <location>
            <begin position="18"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="13">
        <location>
            <begin position="170"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="14">
        <location>
            <begin position="232"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="11">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000939-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="376" mass="42070" checksum="94BCC3A63113345D" modified="2018-12-05" version="1">MKVSSIDCRRLKKIIRKECGSCLIVDCRPYFSFTNSSIKGSVNVNLNSLVVRRSRGGPVPLQFVIPDERALFRLREGSISAIVALDDRTSHWQKLKKDSVAQIVINTLSHLASGASICFLKGGYVNFYSQYPELCTEVKTIDQSGTETEKRVSSHSEKLSHRKPDYDQGKPVEILPFLYLGSAYHASRQDYLSDLHITALLNVSRRDLHPTRGHYDYKWIPVEDSPMADISSHFQEAIEFIDQVKQSGGKVLVHCEAGISRSPTICMAYIMRTQKLRLNEAFEVIKQRRSVISPNFSFMGQLLQFESEVLSNAPAMGATPEPATPCVTESASFFANDFSATFSTKNFEPSMFTLPTSCLQSPVHHPLKLSPITALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7Z0</accession>
    <name>A0A3B5M7Z0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">[histone H3]-trimethyl-L-lysine(4) demethylase</fullName>
            <ecNumber evidence="4">1.14.11.67</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 2-oxoglutarate + 3 O2 = L-lysyl(4)-[histone H3] + 3 formaldehyde + 3 succinate + 3 CO2</text>
            <dbReference type="Rhea" id="RHEA:60208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.67"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JARID1 histone demethylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.67" evidence="4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019610"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019855.1">
        <property type="protein sequence ID" value="ENSXCOP00000019610.1"/>
        <property type="gene ID" value="ENSXCOG00000014729.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157170"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034647">
        <property type="term" value="F:histone H3K4me/H3K4me2/H3K4me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16873">
        <property type="entry name" value="ARID_KDM5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15515">
        <property type="entry name" value="PHD1_KDM5A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15606">
        <property type="entry name" value="PHD2_KDM5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15686">
        <property type="entry name" value="PHD3_KDM5A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Lysine (K)-specific demethylase 5A"/>
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        <property type="entry name" value="Lysine (K)-specific demethylase 5A"/>
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        <property type="entry name" value="lysine-specific demethylase 5A"/>
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        <property type="entry name" value="PHD transcription factor Rum1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.60:FF:000001">
        <property type="entry name" value="Putative lysine-specific demethylase 5b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000001">
        <property type="entry name" value="Putative lysine-specific demethylase 5b"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048615">
        <property type="entry name" value="KDM5_C-hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047974">
        <property type="entry name" value="KDM5A_ARID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047970">
        <property type="entry name" value="KDM5A_PHD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047972">
        <property type="entry name" value="KDM5A_PHD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013637">
        <property type="entry name" value="Lys_sp_deMease-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004198">
        <property type="entry name" value="Znf_C5HC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF17">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08429">
        <property type="entry name" value="PLU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02928">
        <property type="entry name" value="zf-C5HC2"/>
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    </dbReference>
    <dbReference type="SMART" id="SM01014">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="23">
        <location>
            <begin position="16"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="ARID" evidence="22">
        <location>
            <begin position="81"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="21">
        <location>
            <begin position="241"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="24">
        <location>
            <begin position="385"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="21">
        <location>
            <begin position="1109"/>
            <end position="1169"/>
        </location>
    </feature>
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        <location>
            <begin position="1550"/>
            <end position="1604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1268"/>
            <end position="1292"/>
        </location>
    </feature>
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        <location>
            <begin position="1485"/>
            <end position="1526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="1283"/>
            <end position="1292"/>
        </location>
    </feature>
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        </source>
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        </source>
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    <evidence type="ECO:0000256" key="19">
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1629" mass="185635" checksum="A2318AFEC17BEFE9" modified="2018-12-05" version="1">DRVNTSPGYFVPPPECPVFEPSWEEFSDPLGFINKIRPIAEKTGICKIRPPQDWQPPFACDVRNFRFTPRVQRLNELEALTRVKLNFLDQIAKFWELQGSKIRFPHVERKILDLYQLSKIVSSEGGFETVCKEKRWSKVATRMGFPPGKGTGSLLRSHYERFLYPYELFQSGATLTVSGARRSTDVVLFKNTSGHLGPENDVASFLPIALNQGKQRHLKAQAFAIKMRPRKETLEVNFIDLYFCLVCGRGDEEDRLLLCDGCDDSYHTFCLIPPLQDVPKGDWRCPKCVAEECSKPREAFGFEQAVREYTLQSFGEMADHFKSDYFNMPVHMVPTELVEKEFWRLVSSIEEDVIVEYGADISSKDVGSGFPIKDGKRRLLGDEEEYANSGWNLNNMPVLEQSVLTHINVDISGMKVPWLYVGMCFSSFCWHIEDHWSYSINFLHWGEPKTWYGVPASAAEKLEAVMKKLAPELFDSQPDLLHQLVTIMNPNVLMDHGVPVYRTNQCAGEFVVTFPRAYHSGFNQGYNFAEAVNFCTADWLPLGRQCVAHYRRLHRYCVFSHEELLCKMVADPENLDVELAAAVFKEMDDMIDEETKLRQAVQEMGVLSSEQEVFELVPDDERQCYKCKTTCFLSALTCSCSPDRLVCLHHAKDLCDCPLGSKCLRYRYGLEEFPSMLYGVKTRAQSYDTWAKRVAEALAADQKNKKDLIELKVLLEDAEDRKYPENVLFRRLREMVKEAETCSSVAQLLLSRKQRHSRLRSDSSRNRTKLTVDELKAFVEQLFRLPCIISQARQVKELLETVEDFHERAQVALSDEMPDSSKLQELLDLGSGLDVELPELPKLKQELQQARWLDEVRGTLAEPHRVTLELMKRLIDSGVGLAPHHAVEKAMAELQEVLTVSERWEDKARACLQARPRHSMVTLENIVLEARNIPAYLPNVLALEEALQKAKDWTCKVEAIHSGSSYAYLEQLESLLARGRSIPVRLDPLGQVESQVASARAWRERTARTFLKKNSTYTLLQVLSPRVDIGVYSNSKSKRKRVKELMEKERSGYDPDALSDLEENLEEARDPSAVVAAFKAKEQKEVEAIHSLRAANLAKMAMADHIEEVKFCLCRKTASGFMLQCELCKDWFHGACVPLPKTGTQKKVGVSWQSNSKDSKFLCPLCQRSRRPRLETILSLLVSLQKLPVRLPEGEALQCLTERAMSWQDRARQALATEELSSALAKLSVLSQRMVEQAAREKTEKIINAELLKAAANPDLQGHIQTFQQSGFSRAASPRPSVDYDDEETDSDEDIRETYGYDMKDPGEVKPYLFCDEEIPVKSEEVVSHMWPAATPSFCAEHAYSSASKSCVQNLGTPRKQPRKTPLVPRSLEPPVLELSLQAKAQLEDLMMLGDLLEVSLDETQHIWRILQATHPPSEERFLQVMEPDNSLLEKPLKIKFKDSEKKRKRKLERAEQLQKSKELKRVGLELGLGSKPKKKKLKLNLEKNREMKQLAKRLAKEEKERKRKEKAAAKAEAIKDVLEKRKEKKILDIPSKYDWSGAEDSNDENAVCAAKNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSCEMAENEDYICVDCSEKAAGAVDGVALEDYKDNIATLRQRVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M812</accession>
    <name>A0A3B5M812_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">[histone H3]-lysine(4) N-methyltransferase</fullName>
            <ecNumber evidence="14">2.1.1.364</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60264"/>
            <dbReference type="Rhea" id="RHEA-COMP:15543"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="EC" id="2.1.1.364"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:60265"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.364" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M812"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019635"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019880.1">
        <property type="protein sequence ID" value="ENSXCOP00000019635.1"/>
        <property type="gene ID" value="ENSXCOG00000014750.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155281"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044666">
        <property type="term" value="C:MLL3/4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140945">
        <property type="term" value="F:histone H3K4 monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.360:FF:000001">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003889">
        <property type="entry name" value="FYrich_C"/>
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        <property type="entry name" value="SET domain"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SET"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="17">
        <location>
            <begin position="265"/>
            <end position="381"/>
        </location>
    </feature>
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        <location>
            <begin position="389"/>
            <end position="405"/>
        </location>
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        <location>
            <begin position="214"/>
            <end position="252"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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            <dbReference type="ARBA" id="ARBA00022853"/>
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            <dbReference type="ARBA" id="ARBA00023015"/>
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            <dbReference type="ARBA" id="ARBA00023163"/>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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            <dbReference type="ARBA" id="ARBA00023620"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00049353"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50280"/>
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    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019635.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="405" mass="45908" checksum="D6EA33FD54D66C15" modified="2018-12-05" version="1">MLCHAHRPRGTAGQALEHELRCFAVFRRVYVQRDEVRQIATAVQQPELGYTFRVGSLVLHAIGQLTPLQMSAFHSPTTIFPIGYEACRIYWSMRHGNRRCRYVCSVEDNDGFPEFTIRVVEQGYQDLILTDTSPKGVWDKVLGPVSDKRAESGTLRLFPVYLKGEDLFGLTVPAVTRITESLPGVEACFRYSFRYGRNPLVELPLMFNPAGCARAEPRTSSSHTSSSARSNQNVAQGEGGVPHSKTPVVHPRSSQYRWMKSEWRSNVYLACSRIQGLGLFAARDIEKQTMVIEYNGTVLRNEVAIRKAKVYRSQNRAVFMFRIDSEHVVDATQSGGLARYINHSCAPNCVAEVVTFERGYKIIISCNRRVEKGEELCFDYQFDAIEGQHKIACHCRAPECRKWIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M847</accession>
    <name>A0A3B5M847_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein 178A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM178 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M847"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015944"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016143.1">
        <property type="protein sequence ID" value="ENSXCOP00000015944.1"/>
        <property type="gene ID" value="ENSXCOG00000012047.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:negative regulation of osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="T178A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32005:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 178A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32005">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 178B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="10">
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            <begin position="44"/>
            <end position="65"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="10">
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            <begin position="85"/>
            <end position="112"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
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    <evidence type="ECO:0000256" key="3">
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    </evidence>
    <evidence type="ECO:0000256" key="4">
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015944.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="128" mass="13504" checksum="10AF6A100D1B83EA" modified="2018-12-05" version="1">GSYLRRITAGFLGMAAAVLLCGSIVASVGFFWEESLTQHVSGLLFLMAGIFCTISLCTYAASVTYDLSRNPPFIYGLPSDVDHGYGWSICCAWASLGLTVASGCLGTAFPFLSRAGALRSKTARESSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8T2</accession>
    <name>A0A3B5M8T2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Eukaryotic translation initiation factor 4ea</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M8T2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019930"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020177.1">
        <property type="protein sequence ID" value="ENSXCOP00000019930.1"/>
        <property type="gene ID" value="ENSXCOG00000014847.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154194"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Eukaryotic translation initiation factor 4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF74">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="237" mass="27281" checksum="EF3B683BA2511E82" modified="2018-12-05" version="1">MKLASRIQQDNFNGVVRLSHCLPNLLLPELTPNSVPVNSEKENCEKTLQKVVSPEGYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMSGLFYRDGIEPMWEDERNRRGGRWLITLSKQQRKSDLDRFWLETLLCLVGEAFDDHSDDVCGAVINVRAKGDKIAIWTTDFENKDAITHIGRVYKERLGVPPKVIIGYQSHADTATKSGSTTKNKFVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8X6</accession>
    <name>A0A3B5M8X6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transmembrane anterior posterior transformation 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAPT1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M8X6"/>
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        <property type="gene ID" value="ENSXCOG00000014997.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:retina layer formation"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0892">Osteogenesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="164"/>
            <end position="185"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="424"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="466"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="472"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008803"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="533" mass="61316" checksum="3E45A8C2D9BDF3F7" modified="2018-12-05" version="1">MADVLGTELSEEKENEIELKKRQKRPWEEFHRSGKIGITPELPETLGFFDISAVKREQQEVSADPSLTRFICTELTRGYFLEHNEAKYTERRERVYTCMRIPKELEKLMIFGFFLCADAFLYIFTLLPLRVLLALLRLLTLPCCGFSGSRLLQPAQVCDMLKGLILVLCFSLMHYVDYSMMYHLIRGQSVIKLYIIYNMLEVADRLFSSFGQDILDALYWTATEPKERKRDNIGVIPHFFMAVFYVFLHSILIMVQASTLNVAFNSHNKSLLTIMMSNNFVEIKGSVFKKFEKNNLFQMSNSDIKERFTSYVLLIIVCLRNMEQFSWNPDHLWMLFPDVFMVVMSEVAVDIIKHAFITKFNDITADVYSEYRASLAFDLVSSRQKNAYTDYSDSVARRMGFIPLPLAVLLIRVVMSCVKVQGALSYLCVFLFYLGMVTLKVLNSIVLLGKSCVYVKRANMEDKLFERPPTAPSFSAKTDSCPSTSEPQSDAEPPTASPLQEEEADETSDMNHRAPNKDLLEIDRFTICGNRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9M0</accession>
    <name>A0A3B5M9M0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">OBSCN</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9M0"/>
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    <dbReference type="Ensembl" id="ENSXCOT00000020462.1">
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        <property type="gene ID" value="ENSXCOG00000015154.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:protein kinase activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="obscurin isoform X3"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
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        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633">
        <property type="entry name" value="IMMUNOGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633:SF4">
        <property type="entry name" value="MYOPALLADIN ISOFORM X1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF07679">
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
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        <location>
            <begin position="73"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="192"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="286"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="415"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="491"/>
            <end position="525"/>
        </location>
    </feature>
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        <location>
            <begin position="538"/>
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        <location>
            <begin position="549"/>
            <end position="565"/>
        </location>
    </feature>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="733" mass="82514" checksum="7E4FC92B6BD150CB" modified="2018-12-05" version="1">RSINAYLQQPLERIQKYKAFLKDLIRNKARNGQNCCLLEEAYAMVSALPQRSDNTHHVSLIENYPATLEVLGEPIRQGPFQVWEGAPGIRTSSRGHHRHVFLFRNYCIICKPKRDSNTETQAYVFKNMMKLNNIDVNETVEGDDRAFEIWHEREDSVRKYTLQARTVIIKNSWLRDLRELQQRYSITAWSSPDFVEILANCTAELGQTIKLACKATGVPKPVATWYKDGRTVEADPHHIIIEDPDGSCTLILDNMTADDSGQYMCFATSPAGNASTLGKVTVQVPPRFVNKMRNAVFVAGEDAQFTCVIQSAPNPKIRWFKDGRLLTDQEKYQTYSEVRSGVLVLVIKNLTERDLGHYECEVKSDDIAAVLMFSFCLVFLDISCVFANTPVPLLMYSFIYSSEETITTVVKNPRMRRHRSPGLTVPGAHRSETSTPEPPATRPRRMPTPRKTTIPTLYVTEPEGAGARAAESRPRWVEVEEVIEYKVNKSPRLSRRRGISPAGSDRAATPSRPKRSPLDNLNANNSNNKLVEQVQEHLKGDISSQSVSWEEDDPQIASEDEDNEEPGPVVLKQGDRILNLEDLEDYIPGEGETYGSSSSCHVSDEKPCEISVLQREIGGSQVGQPVLLNVGRPDIVPRQRSGFFGRFREHLSNNLFPSASPQGSGARPRTERQVPIQVSHAKLEVKPSYCSEVQRVEGRQQSFKTKVSTQTYGYTSVGKPVTLQIKEKTFQSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9P4</accession>
    <name>A0A3B5M9P4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9P4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020262"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020510.1">
        <property type="protein sequence ID" value="ENSXCOP00000020262.1"/>
        <property type="gene ID" value="ENSXCOG00000015194.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015494"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:L-iduronidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1500:FF:000002">
        <property type="entry name" value="Iduronidase alpha-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1500">
        <property type="entry name" value="Glycosyl hydrolase domain, family 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049167">
        <property type="entry name" value="GH39_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049166">
        <property type="entry name" value="GH39_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051923">
        <property type="entry name" value="Glycosyl_Hydrolase_39"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12631">
        <property type="entry name" value="ALPHA-L-IDURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12631:SF8">
        <property type="entry name" value="ALPHA-L-IDURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01229">
        <property type="entry name" value="Glyco_hydro_39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21200">
        <property type="entry name" value="Glyco_hydro_39_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolases family 39 N-terminal catalytic" evidence="3">
        <location>
            <begin position="75"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-L-iduronidase C-terminal" evidence="4">
        <location>
            <begin position="298"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="327" mass="36393" checksum="4DF72AA30B9BAFDF" modified="2018-12-05" version="1">MSVNTVPRQSLAHKMIWLFTFNGRDGLNFSLCSLAMLCIHEASFSPLLGLVVVVLVLLELLRCQLAHRVVKMKELQHQDLLLASPNNTINYTLLSNDNAFLSYHPHPFTQRTLTARFQVNNTRPPHVQLIRKPVLTVMGLLALLGETQVLAQVLNSGRQENSTVGVLASTHRPLIHGGSDSWQAAVLVYNSDDNSTSQLSVCYPGLLYVTYYMDNNVTNPQQLWQSMGSPDFPTAQQFRQLRSVEDPRTDGPQKLPPGDTLTLKAKLSLPSVLLVHVCARPEVGPDQVGKSLRLLDQKVGGLYRVRAVDYWGRAGPYSLPERFSDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9S8</accession>
    <name>A0A3B5M9S8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9S8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018031"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018258.1">
        <property type="protein sequence ID" value="ENSXCOP00000018031.1"/>
        <property type="gene ID" value="ENSXCOG00000013581.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159484"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046931">
        <property type="term" value="P:pore complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03158">
        <property type="entry name" value="penumbra_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000007">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF154">
        <property type="entry name" value="TETRASPANIN-33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="154"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="282" mass="31318" checksum="80217287ED813553" modified="2018-12-05" version="1">LALRGATGSHKDFTFVSSVVKYLLFFFNFIFWVISLVMVAVGVYARTVKHAEATLASLSVDPAVMLMVVGVLMFLITFCGCVGSLRENICLLQTFCISLVVIFIIQLAAGILGFIFSNKARQKVTEVINDAVVHYRDDVDLQNLIDFGQEEFGCCGAATYLDWSNNIYFDCNQSNPSRERCSVPFSCCIRFLSVQSVVNTMCGQGMQEREFGEAGKKIHTNGCIDVLVDFIHSNMFILGGIALGLAIPELVGMFLSQLLINQIKDQIELQQYNLKHSSDPWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAZ3</accession>
    <name>A0A3B5MAZ3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cationic amino acid transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027889911.1">
        <property type="nucleotide sequence ID" value="XM_028034110.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027889912.1">
        <property type="nucleotide sequence ID" value="XM_028034111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAZ3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020757"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021010.1">
        <property type="protein sequence ID" value="ENSXCOP00000020757.1"/>
        <property type="gene ID" value="ENSXCOG00000015564.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114154749"/>
    <dbReference type="KEGG" id="xco:114154749"/>
    <dbReference type="CTD" id="6545"/>
    <dbReference type="GeneTree" id="ENSGT00940000154637"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000010">
        <property type="entry name" value="probable cationic amino acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF4">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="7">
        <location>
            <begin position="560"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="420"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="420"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="669" mass="72530" checksum="7B044555B462A759" modified="2018-12-05" version="1">MATCSTGCAPAVRLCQRLNRLKTLDGDMMATSLKRCLSTLDLTLMGVGGMVGSGLYVLTGTVAKDTAGPAVIISFVFAGIASLLAAFCYAEFGARIPKTGSAYMFTYVSVGEVWAFLIGWNVILENMIGGAAVARAWSGYLDSIFNHAIQNFTETHIMQWNVPFLAHYPDLLAAGILVFASVFISFGVQVSSYLNHIFSTISMGVIAFILVFGFVLADPANWSEEQGGFAPYGMSGILAGSATCFYAFVGFDVIASSSEEAKNPQKAVPIATAISLGLAATAYILVSCVLTLMVPWRTLDPNSALADAFFRRGYSWAGIVVAVGSICAMNTVLLCNLFSLPRIVYAMAEDGLFFSIFARVNPVTKVPVNAILVFGILMAGMALIFDLEALVQFLSIGTLLAYTFVAASVIVLRFQPEKTNSNDTTSPNVNAEVSPAPSESQTITEDSEEPKQYESFSDKLQLVERQKTRERRGVGHLKAYWEPYLDRLLGDCEPGEVVAFCVMTLIVSSVSLCAVLEFGNGQLQFPVWCFTMLLVIFTSAYVLSLALIWIHEPQRNNQTFQVPLVPLTPGASILFNVFLMMKLSLLTWIRFTVWIAIGLFVYFGYGIWHSKEGLRELQPKDMAARYVVLPSGSLVETVQSVQPDGQVVTSAHQISSSAALTATEAAGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MB85</accession>
    <name>A0A3B5MB85_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Si:dkey-78k11.9</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MB85"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021107.1">
        <property type="protein sequence ID" value="ENSXCOP00000020852.1"/>
        <property type="gene ID" value="ENSXCOG00000015616.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155094"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008188">
        <property type="term" value="F:neuropeptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15967">
        <property type="entry name" value="7tmA_P2Y1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027294">
        <property type="entry name" value="NPS_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24244:SF0">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24244">
        <property type="entry name" value="NEUROPEPTIDE S RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="6">
        <location>
            <begin position="33"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="322" mass="37156" checksum="9C9331B0E4DD9F2D" modified="2018-12-05" version="1">MNTSTCTKPNKKLFEHTILPALYILVFVVGLVLNVWGMKSLLHNWKKLRSINILVLNLGLADILYLLTLPFLIVYYFKGNKWIFGDGFCKVTRFCYNLNLYGSIGFLTCISVYRYLAVVHPMRVMGKLTTTHSVVISVIIWILVSVQSLPDMFYPKTYPNGSKCFDTTSYEHIESYLDYTIAWTFVGFCIPFVTIVGCYGHVTVVICRSNMMKKDQKRQILKLMAIVILLFSLCYAPYHIFKNLSLYSRVLRKQRTCPKWDSGVFIARQVTRGLVSLHNALNPLVYLFVKHGGGSIILRLFLPLVNWTLSELEEIHNKFKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBJ8</accession>
    <name>A0A3B5MBJ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">non-specific protein-tyrosine kinase</fullName>
            <ecNumber evidence="5">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBJ8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021618"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021882.1">
        <property type="protein sequence ID" value="ENSXCOP00000021618.1"/>
        <property type="gene ID" value="ENSXCOG00000016162.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155113"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007172">
        <property type="term" value="P:signal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13190">
        <property type="entry name" value="FERM_C_FAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05056">
        <property type="entry name" value="PTKc_FAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.330:FF:000001">
        <property type="entry name" value="focal adhesion kinase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000058">
        <property type="entry name" value="focal adhesion kinase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000021">
        <property type="entry name" value="focal adhesion kinase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000047">
        <property type="entry name" value="focal adhesion kinase 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000039">
        <property type="entry name" value="Focal adhesion kinase, isoform D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.540">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041390">
        <property type="entry name" value="FADK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049385">
        <property type="entry name" value="FAK1-like_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041784">
        <property type="entry name" value="FAK1/PYK2_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036137">
        <property type="entry name" value="Focal_adhe_kin_target_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005189">
        <property type="entry name" value="Focal_adhesion_kin_target_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46221">
        <property type="entry name" value="FERM AND PDZ DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46221:SF9">
        <property type="entry name" value="NON-SPECIFIC PROTEIN-TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21477">
        <property type="entry name" value="FERM_C_FAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18038">
        <property type="entry name" value="FERM_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03623">
        <property type="entry name" value="Focal_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68993">
        <property type="entry name" value="FAT domain of focal adhesion kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="FERM" evidence="21">
        <location>
            <begin position="1"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="389"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="421"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="970" mass="109888" checksum="9A533D294956123E" modified="2018-12-05" version="1">MASSSYLEPSLNHSTPGGCGVKSRGSAGGLERPPGSMDRVLKIFHYFETNSEPSTWASNIRYGDATDVRGVIQKIAEIHKLRCVSSLGLRLTHLHSDVLHWLHPDLGVSHVRERFEKQHPQEEWRYELRIRYFPKGYLSHFSEDKPSLNYLYHQMPGNALDKKSNYELLEKDVGLRRFFPKSLLDSLKAKTLRKQIQQTFKQFANLNDEQSIHKFFEIITPIYPFDKECFKCALGSSWVISVELAIGPEEGISYLTDKGSTPTHLANFNQVQSIQYSALEEKDRKGMLQLNVAGAPEPLTVTTALLNTAENMADLIDGYCRLVSSVTRSFIVRVHKGMLSSYIVLYFDVPLSLCFQCCCETDDYAEIIDEEDTYTMPSTRDYEIQRDCIELGRCIGEGQFGDVHQGVYISQENPALSVAVKTCKNSTSDSVREKFLQEALTMRQFDHPHIVKLMGVITENPVWIIMELCTLGELRSFLQVRKYSLDLATLILYSYQLSTALAYLESKRFVHRDIAARNVLVSTVDCVKLGDFGLSRYMEDSSYYKASKGKLPIKWMAPESINFRRFTTASDVWMFGVCMWEILMYGIKPFQGVKNNDVIGRIENGERLAMPPQCPPTLYSLMTKCWSYDPSKRPRFNELKTQLRYCSLLDYLAVFMWFMPVSESPPVPSRPGYPSPRSSEGFYPSPQHPVHYQMAAYPGPHTLPSVPSALYPPQAAMVDTHHDRAVDMQQCVGQQCLLMEEQLMIQQQQMEEDQRWLEQEERLLVTHSAHSGTCTLAHLTHAQHNGDHVVPPKKPPRPGAQSQVGSLACLNTGDSYNDGVKLQPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYIPMVKDVGLALRTLLATVDETLPQLPASTHREIEMAQKLLNSDLAELIGKMKLAQQYVMTSLQQDYKKQMLTAAHALAVDAKNLLDVIDQSRLRMMAHSRPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCC6</accession>
    <name>A0A3B5MCC6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rap-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCC6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021195"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021455.1">
        <property type="protein sequence ID" value="ENSXCOP00000021195.1"/>
        <property type="gene ID" value="ENSXCOG00000015860.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156138"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050989">
        <property type="entry name" value="Rap1_Ran_GAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711">
        <property type="entry name" value="RAP GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711:SF3">
        <property type="entry name" value="RAP1 GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21022">
        <property type="entry name" value="Rap-GAP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="179" mass="20882" checksum="77B596AD4EB47F82" modified="2018-12-05" version="1">FFPQTEEDYIPYPSVHEVLGRRSPFPLILLPQFGGYWIEGTNHEPKDPPEADHPPCPASHIKLETNSTAKIYRKNFMGKEHFNYYTMDAALSHLVFSMKYEVIGDQEHLRLMLRTKYKTYHDVIPISCLTEFPNVVQMAKLVCEEVNVDRFYPVLYPKVAVFFPPFPCHPSTRNHHDVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCC8</accession>
    <name>A0A3B5MCC8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCC8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017354"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017574.1">
        <property type="protein sequence ID" value="ENSXCOP00000017354.1"/>
        <property type="gene ID" value="ENSXCOG00000013075.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156462"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000003">
        <property type="entry name" value="Collagen type VI alpha 3 chain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF13">
        <property type="entry name" value="COLLAGEN ALPHA-3(VI) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="VWFA" evidence="9">
        <location>
            <begin position="73"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="9">
        <location>
            <begin position="331"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="9">
        <location>
            <begin position="499"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="712"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="723"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="736"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="748" mass="82851" checksum="95FDA473F69D7218" modified="2018-12-05" version="1">MDGSFTFLCWQFSGRCAESEFREGSLTGLVLITFCRLSKGLLALLGFASCLSAVSTQDLELLLPGSAAGPRKDVVFLIDGSDGVGREFAIIQEFIRRVVESLNVGENKIRIGVVQYGDSAKADMHLNTYTTKEGVLNGIREMRQIGGRQRNLGQALQFLSQNVMIPARGSRKQEGVPQFAIVVSSRPSTDDITRQASSLKQSGVISFSIGTREINPTELKVVSYVPNFSFTVDDLPGLYIVQENLINTITELSDEDIAKLRPQFPIYEGKYAETSDCWAISKGELPGPIRVSTCPMFLILHPSKGALIIAFYFACILTEFTPPAATGDKRDVVFLIDGSSATRSEFPSVRDMIRRVVEQLDVGLDRVRVSVVQYSDDPKPEFLLNEHSTKAEVRQAVMKLRSSGGNRLNTGRALEWVSRNIYQRSAGSRIEEGVPQFLILVTGGRSSDDVSTQANQLKRNQVAPLAIGSRNADLNELRQISLKPELVYSVDTFQQLPRDIIFLIDGSDTTGPNGIAHIRDFIVSIVQQLDVQPDKVRVAVVQYADRAKTEFSLNTHNNKQAVLSAVKRLRQMGGRSSQLANAIDHVLENEVKPLAGVRLSDASQHLVVLTGGPSSQPVSISGPLLKNKRVNCIGVGTGNADRSQLSQIATSSEDVVTVPTFPNLPTAKDKVISRLRGSTGTAFPTYVYPKRQPTNRPCAVCHRCERRLFPQHLQQQGQRHRSYRQDRIQRRTQNHDRRRHSPRARRPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCI9</accession>
    <name>A0A3B5MCI9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DIX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCI9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018986"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019223.1">
        <property type="protein sequence ID" value="ENSXCOP00000018986.1"/>
        <property type="gene ID" value="ENSXCOG00000014273.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182903"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.130:FF:000001">
        <property type="entry name" value="Segment polarity protein dishevelled homolog DVL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001158">
        <property type="entry name" value="DIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038207">
        <property type="entry name" value="DIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015506">
        <property type="entry name" value="Dsh/Dvl-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10878">
        <property type="entry name" value="SEGMENT POLARITY PROTEIN DISHEVELLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10878:SF5">
        <property type="entry name" value="SEGMENT POLARITY PROTEIN DISHEVELLED HOMOLOG DVL-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00778">
        <property type="entry name" value="DIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00021">
        <property type="entry name" value="DAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50841">
        <property type="entry name" value="DIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="DIX" evidence="6">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="82" mass="9274" checksum="A1376E584374D9B9" modified="2018-12-05" version="1">MAETKIIYHIDEEETPYLVKLSVPPEKVTLADFKNVLNNRPVNSYKFFFKSMDQDFGAVIVSIVGGSDSTEPNITYFHCVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCM5</accession>
    <name>A0A3B5MCM5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome c oxidase subunit 7A1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c oxidase VIIa family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027901170.1">
        <property type="nucleotide sequence ID" value="XM_028045369.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCM5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017444"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017666.1">
        <property type="protein sequence ID" value="ENSXCOP00000017444.1"/>
        <property type="gene ID" value="ENSXCOG00000013156.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114161789"/>
    <dbReference type="KEGG" id="xco:114161789"/>
    <dbReference type="CTD" id="1346"/>
    <dbReference type="GeneTree" id="ENSGT00940000154550"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097250">
        <property type="term" value="P:mitochondrial respirasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905178">
        <property type="term" value="P:regulation of cardiac muscle tissue regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002082">
        <property type="term" value="P:regulation of oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008535">
        <property type="term" value="P:respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00928">
        <property type="entry name" value="Cyt_c_Oxidase_VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.91.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 7A1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.91.10">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039297">
        <property type="entry name" value="COX7a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036539">
        <property type="entry name" value="Cyt_c_oxidase_su7a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003177">
        <property type="entry name" value="Cytc_oxidase_su7a_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10510">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10510:SF5">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 7A1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02238">
        <property type="entry name" value="COX7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81419">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="81" mass="8938" checksum="1E04517C0CBE3677" modified="2018-12-05" version="1">MNRLLNVPVAAVRAFSSSSRNLKNKVPEAQKLFQEDNGLPVHIKGGTSDVLLYRATMLLTAAGTCYSLYWLLVASMPNKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDN8</accession>
    <name>A0A3B5MDN8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">THAP domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. Specifically binds the 5'-[AT]NTNN[GT]GGCA[AGT]-3' core DNA sequence and acts by modulating expression of pRB-E2F cell-cycle target genes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the THAP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDN8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018038.1">
        <property type="protein sequence ID" value="ENSXCOP00000017814.1"/>
        <property type="gene ID" value="ENSXCOG00000013416.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000176864"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001935">
        <property type="term" value="P:endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026516">
        <property type="entry name" value="THAP1/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46600">
        <property type="entry name" value="THAP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46600:SF11">
        <property type="entry name" value="THAP DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="110" mass="12474" checksum="3A817686FEC02AFB" modified="2018-12-05" version="1">MVCSCAFPGCANRANSWSPYRFHRIPLADLHLRMLWLSVLGMDVNTPPAALKHLRSLPKKIVVCANHFTTDCFENYRQYTAGFALRLRLKKGSVPTIRFKFEDDGDVSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5ME50</accession>
    <name>A0A3B5ME50_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Fibroblast growth factor receptor</fullName>
            <ecNumber evidence="19">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5ME50"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021820"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022084.1">
        <property type="protein sequence ID" value="ENSXCOP00000021820.1"/>
        <property type="gene ID" value="ENSXCOG00000016278.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155860"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017134">
        <property type="term" value="F:fibroblast growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005007">
        <property type="term" value="F:fibroblast growth factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05053">
        <property type="entry name" value="PTKc_FGFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000007">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000016">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000020">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000011">
        <property type="entry name" value="Fibroblast growth factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016248">
        <property type="entry name" value="FGF_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF131">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000628">
        <property type="entry name" value="FGFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 19 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 22" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 19 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 19 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13 19" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="273"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="26">
        <location>
            <begin position="56"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="26">
        <location>
            <begin position="156"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="375"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="520"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="381"/>
            <end position="387"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21 23">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="459"/>
            <end position="461"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="524"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="538"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="81"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="178"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR000628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000628-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000628-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000628-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="695" mass="78030" checksum="F9FD67DFB6832C56" modified="2018-12-05" version="1">MCTTCVCTWSQSHSALSCYCYSCSRSLLQKYICICLTYLFQLSLCCDLMIVSALAPQWVMPEKMEKQLHAVPASRTVKFRCQATGNPPPSLRWYKNGKEFRKDQRIGGFKFREHMLIMESVVPSDRGNYTCVVENVHGSLKHTFLLDVIERSPHRPILQAGLPANQTAVVGTNVEFVCKVFSDPQPHIQWFKHITVNGSKQGADGHPFVMVLKAAGLNTTEKEMEVLTLKNVSLDDAGEYTCLAENSIGVSYHSAWLTVLLPSPVPSQTYLEIFIYCLGFFVIIILTVSAVMCRLCCAQKKRDFNSQLAVQKLAKSIPLRRQVRLVSVESSTSQQSGMCLMHQSRLSSAATTILAGVSEYELPYDPGWELPRERLTLGKPLGEGCFGQVVLAEAIGVDKNKPTRLTKVAVKMLKADATEKDLSDLISEMEMMKMIGKHKNIINLLGACTQGGPLYVVVEYASQGNLREYLRARRPAGLEYWSGLRQASLGSLEIRELVSAAYQVARGMSYLASKKCIHRDLAARNVLVTEDNVMKIADFGLARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHRMEKPSACTQELYLMMRECWHAVPARRPTFQQLVEDLDRTLSNMANQEYLDLAVPLAHRTSTASSWERLLPVSCAARSSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEE7</accession>
    <name>A0A3B5MEE7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PARP catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEE7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021925"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022192.1">
        <property type="protein sequence ID" value="ENSXCOP00000021925.1"/>
        <property type="gene ID" value="ENSXCOG00000016384.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183129"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051838">
        <property type="entry name" value="ARTD_PARP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21328">
        <property type="entry name" value="POLY ADP-RIBOSE POLYMERASE FAMILY, MEMBER PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="107" mass="12106" checksum="90554E0B10543DF2" modified="2018-12-05" version="1">MGKSQHQIPTREELKKKYNRINKIKQEEPGQPRFLQSSNLSCIALCEVITSKALQKHGNIWVCPVSDHVCTRFLFVYENGQVGDVHINTQDAQIQTEILQATGSKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGE8</accession>
    <name>A0A3B5MGE8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand calcium binding domain 14</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGE8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020186"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020433.1">
        <property type="protein sequence ID" value="ENSXCOP00000020186.1"/>
        <property type="gene ID" value="ENSXCOG00000015131.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011196"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042352">
        <property type="entry name" value="EFCAB14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15717:SF2">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15717">
        <property type="entry name" value="PROTEIN KIAA0494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="26182" checksum="47063A53A99209A5" modified="2025-10-08" version="2">MTPQKKMKKRKELNALIGLGDSKRKKTKKGSGHRLLRTEPPGSESESSSEDDDFNNLNGGTNFGKRSYSQCCNVCYPLFLFIILAACVMACAGLIWMQIALKKDLDSLKEKLHSMESSQKVSSSEIPKISEDLKNKEKKLEDIENGDKGLSKLWSNLTEMNRKITSLDSAVNHLKTSLKSSADLIGLPTTVEELQKSVATIGSTLTSVQHDVETMKASLENQKKDDESKKTMVILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHG6</accession>
    <name>A0A3B5MHG6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHG6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023060"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023337.1">
        <property type="protein sequence ID" value="ENSXCOP00000023060.1"/>
        <property type="gene ID" value="ENSXCOG00000017222.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016206"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016422">
        <property type="term" value="F:mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099122">
        <property type="term" value="F:RNA polymerase II C-terminal domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000036">
        <property type="entry name" value="Phosphorylated CTD-interacting factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039881">
        <property type="entry name" value="PCIF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022035">
        <property type="entry name" value="PCIF1_WW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21727:SF0">
        <property type="entry name" value="MRNA (2'-O-METHYLADENOSINE-N(6)-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21727">
        <property type="entry name" value="PHOSPHORYLATED CTD INTERACTING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12237">
        <property type="entry name" value="PCIF1_WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="43"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="646"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="646"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="663"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="685" mass="77246" checksum="12E8F657D28E6859" modified="2018-12-05" version="1">MSNNSQGSVKGEAATMVPPSGSASSHGPPLSPSTTSKSPELPELVQAGWSKCWSRRENRPYYFNRFTNQSLWEVPVLGQHDVIFQSDPLGLNAAPAEAGDGNLGNGQRKRRSSEEQGAGPNSFKRTKPTTPISPSTPGVKPWSSAPEEKQVPATTPTTPSPAPAPYRPAIYWDLDVQTNAVIRERSAANHLPSHPEIELQRAQLVTKLRQHYHELCHQREIDPPRESFNRWLLERKVIDKGLDPLLPSECEPVVSPSMFREVMNDIPILSRIKYKEEARKLLFKYAEAAKRMIDSRNASPESRKVVKWNAEDTMNWLRRDHSASKEDYMLQDRLEHLRQQCGPHVTAVAKDSVEGICSKIYQLSAEYSRRLRQTHLSLLQRLVYCYPVRLSIPTPALPRVELHFENDVACLRFRGEMVKVNRGHFSKLQELLYRYSCIDDPRFEKFLARVWCLLKRYQQVMFGSGANEGSGLQGALPVAVFETLNRQFGVTFECFASPLNCYFKQFCSAFPDIDGFFGSRPVSGSFEANPPFCEELMDAMVTHFEELLDRSSEPLSFIVFVPEWRDPVTPALTRMEASRFLRHQLNVPAYEHEYRSGSQHICKDDMYYRAVHGTAVLFLQNDAGFGKWAPTPERLAELTAAYRPSLSRTSSLSSPGPAQSAPGDRDSTPKASDRTQSAMMSPGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHZ4</accession>
    <name>A0A3B5MHZ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NAD(+) diphosphatase</fullName>
            <ecNumber evidence="2">3.6.1.22</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>NAD(+) + H2O = beta-nicotinamide D-ribonucleotide + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11800"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.6.1.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:11801"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>NADH + H2O = reduced beta-nicotinamide D-ribonucleotide + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48868"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90832"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.6.1.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:48869"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>NADPH + H2O = reduced beta-nicotinamide D-ribonucleotide + adenosine 2',5'-bisphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60820"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:90832"/>
            <dbReference type="ChEBI" id="CHEBI:194156"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:60821"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="3.6.1.22" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHZ4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023933"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024217.1">
        <property type="protein sequence ID" value="ENSXCOP00000023933.1"/>
        <property type="gene ID" value="ENSXCOG00000017869.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158879"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03429">
        <property type="entry name" value="NUDIX_NADH_pyrophosphatase_Nudt13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015375">
        <property type="entry name" value="NADH_PPase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049734">
        <property type="entry name" value="NudC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001299">
        <property type="entry name" value="PRK00241.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11383:SF3">
        <property type="entry name" value="NAD(P)H PYROPHOSPHATASE NUDT13, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11383">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE-LINKED MOIETY X MOTIF 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09296">
        <property type="entry name" value="NUDIX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="10">
        <location>
            <begin position="184"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="333" mass="37733" checksum="4A28E10C15BD4476" modified="2018-12-05" version="1">MYILCYLCIQSIRYCFLYCSRRYINRLKENDAACLSALQNGHIFLFHRLIPLLQCSDPETFRPIALTYSEVQSVLHKCGSDGSLLKESILIGCSEQNHAQFCLDVGKSMLDSFSIAQNCKGTFMDLRKAFFLLEGAEAPLVAKAQALLQWHQINRFCGATGQLTQRNQAGSQRACSSSSIVYYPKMSPVVIVLVSDGKRCLLGRQSSFPRGLYSALAGFCDMGETLEEALRREVAEEVGLEVQNIIYSSSQHWPFPHSSFMLACHATVSPLNVDHLELEDARWFTEEEITCALQVKAPPWRDHRPAVWLPPKHAVAHRLIMEWREQQHSQDTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJ10</accession>
    <name>A0A3B5MJ10_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJ10"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021365.1">
        <property type="protein sequence ID" value="ENSXCOP00000021106.1"/>
        <property type="gene ID" value="ENSXCOG00000015790.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177072"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017294773" description="Secreted protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="91" mass="10598" checksum="2C9E1D387559ABFC" modified="2018-12-05" version="1">MVQSGCSILLNTWQLLVVTELAPAHHSRENIQRSLTTCRFTLMPLFRRFLGFAVLDRLTLPSKQGDFHHLFHKKVPCKLCMYLNLNFLPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJR8</accession>
    <name>A0A3B5MJR8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ERI1 exoribonuclease 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Exonuclease domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ERI2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJR8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021371"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021632.1">
        <property type="protein sequence ID" value="ENSXCOP00000021371.1"/>
        <property type="gene ID" value="ENSXCOG00000015998.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063205"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06133">
        <property type="entry name" value="ERI-1_3'hExo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000062">
        <property type="entry name" value="ERI1 exoribonuclease 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051274">
        <property type="entry name" value="3-5_Exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047201">
        <property type="entry name" value="ERI-1_3'hExo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044">
        <property type="entry name" value="3'-5' EXONUCLEASE ERI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044:SF61">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="14">
        <location>
            <begin position="593"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="240"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="407"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="494"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="428"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="510"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="671" mass="74155" checksum="1BF53722DB1BA7C1" modified="2018-12-05" version="1">LILFSCKRRLGLLRQRSQSASGLKKPVKSNQIFSYLIVIDFESTCWREKNNYGQEIIEFPAVLLDTSSGEVESEFHTYVQPQEHPVLSEFCTELTGITQVQVEAGVPLQICLSQFNRWVQNLRLEKGVMFPNKQQASSASDESQKLCTFLTWSDWDLGVCLQYECKRKQLHKPDVLSSWIDLRSTYRLFYSRKPKGLNGALQDLGIQFSGREHSGLDDARNTARLAIRMMRDGCVLKTTRSLERVSGPPMTKSSSSSNILLKARQKRPPHGEPPSSSSGQTCHSLISPKTLLNTTPAPLKGQTDKLVTAHVAPRLLLSTVPSNCSSPCGNGSSSHVLFSTTVGSISSLPHVVKTSPVDEEERDLLVETNERCGSYDDVVLISDQTETDTISGCEVGYFSDDDGRCQAWDGMDHSPEAPLASQRERTENPNVPNNTRDSSMAWKSVNSNVEVFNHSREEPYQSTCRQSEKCFAVPKVVNWSKLNGHSRLDTCHKNNQNHEVSPVLTNPFLPENTSTPNPSTTRPKPTTAKHRKPLKSSFTIYTDPKTPRPATYSSLHPPRSILSSLPTNIPFSCAKRLSGALTAKGSKVTSPLCACGRRARRQVVSNGGPNHGRGFYCCPVRCSGGKERGCGFFKWESALTKSSSVAPPAAGSSLCQVNSALPYRRPQRKSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MK60</accession>
    <name>A0A3B5MK60_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycine amidinotransferase</fullName>
            <ecNumber evidence="10">2.1.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">L-arginine:glycine amidinotransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the biosynthesis of guanidinoacetate, the immediate precursor of creatine. Creatine plays a vital role in energy metabolism in muscle tissues. May play a role in embryonic and central nervous system development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-arginine + glycine = guanidinoacetate + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:13201"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57742"/>
            <dbReference type="EC" id="2.1.4.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Amine and polyamine biosynthesis; creatine biosynthesis; creatine from L-arginine and glycine: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the amidinotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.4.1" evidence="10"/>
    <dbReference type="RefSeq" id="XP_027869557.1">
        <property type="nucleotide sequence ID" value="XM_028013756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MK60"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024744"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025045.1">
        <property type="protein sequence ID" value="ENSXCOP00000024744.1"/>
        <property type="gene ID" value="ENSXCOG00000018486.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114142460"/>
    <dbReference type="KEGG" id="xco:114142460"/>
    <dbReference type="CTD" id="2628"/>
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        <location>
            <begin position="1831"/>
            <end position="1857"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1872" mass="196746" checksum="F8BBEBE660018193" modified="2018-12-05" version="1">MSERSGQTAKGKEGKTKYASLNLFDTYKGKSLETQKPVVPPRHGLQSLGKVASARRMPPPANLPSLKAENKGNDPNVSLVPKDGTGWASKQEPADPKSTDALSAPQPESQQPVASQIAAPTRPRTPPISEAVGARSWAQASVTHGTQGDGGKGSNLPSPFSREEFPTLQSAGDQDKAGREPATADQWYGPGPSLRPQNVTSWRDGGGRALAPTLPGEGAAEGGTGGALVMDGAAGVPHPNPQIQGPPRNPPAGSPALPLPQPPMGPGFPPFRGIMPPFMYPPYLPFPGPYGPQGPYRYPPPGEGPPPRFSRGQGPDSRAQGGPRDSGEMVKRPSILKQDDLKELDELDHDGDDGWAGANEEIDYSAKLKFSDDEADEEGDEDKRESKNDSREQQRSQDAPTANSHSRASDSGGESRHTPPSNADGAPQPPSSKPGWAEEGSSGWGNQGAPSSYQGRRPGLGGPREQPSPPPGPLLGPGPYSFYRQDRPHGQGSLLGPGKPAAAAAQLQPASAGPAASSQPGLLVHGSQGDDEDETWRQRRQQSSSEISAAVERARRRREEEERRMEEERRAACAEKLKRLDEKQQQQQGSGPAAAAGSSKTPASSPNISQPASPCVDPEEPPVLAAQSGSGSGASDRRRASSNSSYDSTADVQQCPQPAVSQPQQPTLDVPPSVESKEESAGSSHVRAGSGSERGVEPVKMESSGVGVGRQAGGPPGQGYSKYQKSLPPRFQRQQQEQLLKQQQQWQQQQHSQASQSQLSPQPQAPQGPSPGSTPQPGPGPKQGGPLYQPGSMVRPPPLPMNFDPRWIMMPYMDPRVMQGRPPPMEYYSAGMHPSGLIGRERSDSGGSGSEPFDRQQQHPGHPHRGTPPIDPKLAWGPEVFPGGADNRGLTSPLRQKQEEEDVGKGPRSDTPPHRLRDGGLGPVQQPSSSSGTSSQTPPPPVGVQVGSQGGTHHPHHYIGGRGNYSNFPDQGARMPPHPQQRQGERGNQPHGFTHPDDGPSRGSQQVQIWGAPHSHYDRNGRADLPTVEGSAHLPHHHSHHHQQPQFPLNPHKVENSRDRGGEAPAKKTDSSPPLHQPSLSSSCSSSSSSAREDGKAAQHHPPPLREGEAVVGERGSNSHMKPDKPGPTFLAHSNQNPLQHGGHNQTQQHPHSKSNQRGGREHKTETQWGPRPGSNSMGGGSSHGRRGNNAGGGRGEDPSSTPSDHKSSNQTGGNNPNKRAGPIKKPLLKEIKREGVEVDGGDKQGKEKEQDGGQPASMKQDASSMPQNASTATKEEQNHTAKARSGGKERASGGGGGSGRGSKEVDSLSSGFPSRRDRDRSFERGGMPAKGGRPSRGRGGEFYRGGRGYRGTYTASAGPAGGGRGRMGARSGRDYRSSTGGGHHQEAKGDGSGVRHGQDRSQHNPARARNRSETRSEGSEYEEIPKRRRERGSETGSESGGSDLGHSDKEEHQKPNSKNCSGNAGATGNVSSVAPRVSQARVFTPRGVPSRRGRGGGGGGGNMYRGSGTVGGPVGSHRVGPGSGSYAGSSKSATSGRKQQGPPLTSGPKDLGRGGGAGEKKDKVVDGGQNQGVNPPPPSLPATAPPAVASAENGGVIIQQAATNPAPNSNTPPHPANRGHPPGGFDRPPRRRRHGRSQHQQDKPPRFRRLKERENAARINGGVGVIGGGRPSSPSLNSVQDSNGGPAVAPVAGNAPNHGSSLSTNNNNNSGGQPNNTNNHHYYNQSNAAPAHAQHHHNHGAKSPDFTNQNSDQANEEWETASESSDFTEFRDRDAGGGKSYVSHHHHHPGRGGGGGLVDRDMAGKEPSANKRSFSSQRPGMERQNRRVSAGGGGGRGPRGPPGGGGSGAPANGGGNRGEKRGNWPSPKNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MS26</accession>
    <name>A0A3B5MS26_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nucleolar protein 8</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MS26"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022289"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022562.1">
        <property type="protein sequence ID" value="ENSXCOP00000022289.1"/>
        <property type="gene ID" value="ENSXCOG00000016657.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004860"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902570">
        <property type="term" value="P:protein localization to nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48029">
        <property type="entry name" value="NUCLEOLAR PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48029:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="229"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="291"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="391"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="627"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="756"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="821"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="561"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="308"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="343"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="445"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="471"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="503"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="522"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="539"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="636"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="649"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="674"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="687"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="703"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="763"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="787"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="821"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="844"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="866"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="893"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="904"/>
            <end position="918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="918" mass="103181" checksum="F4296944C9843071" modified="2018-12-05" version="1">MLFSYFFPADVPYKTFAYININISEADLKKCMTVLNKSKWKGGTLQIEAAKESFLQRLAQERQEAAEHQLQRSANGAKKHKLLDTWSKAGVQNFTMKAAVPGSEVPGCKDWVVSKFGRVLPVLQLRCQKGSKARTVKYDPSKYCHNIRRLDRTDPDETLSVTQLTWQVEGGDDDISKRRRGEFPPYEPIKPKKSRTDPVFLHKTATDKITAQREDRWFLDSDVDSDEEVRSLAAAQQSSHDALQQEAEDDDNLEVVGLDYLVKSGPNGNRNSLNGDFQSICHQYVFPDEEDYDSAGTDELFDSRRNPKTTPGDVSSSKNSSSLQRENPAGVRPAMKHSSDSHSDEDDEEEEGNSSDSSSDFDFPALFSANSNHLRISLADLQRLAEGKVPSILGSGLKPESGFPVRHARKEAITPDEILASILGEDSSSDDKEHKKKKKKAEGVTSASLQPSEETGPETSQTDRCLRRKREHEQDEAAGSERQKHGETSEALNDPQTNSSSGSEEDEEEEEKMISEEEEQEAGAAPHPAPAAGSKTSSSEEEEEEEAEEEVAPPWVALAAKEEEELQRKANMRRLAALQQRQKEAEEHQKFIQGALANLLWFWSKRLLSSNHPSSFSTSVCFQERLKPSGPQLFADSEDDEEGGEEEDGSRFDIRPEFEGPAGQKLMELQSRFGTDERFRMDSRFLEDEEEEEETDVSALHYDPSKEEHAAFETKTEETKKESKAARRKKREEAQKLPEVSKEIYFQVSGDLKAVFGQTNDGSAEEEEGANWDKVEEEEGGGEEEQLPPSLLSADPSSKSEESSGFRFSFFGDESAAGTEYKAEKMKAPKVSWHQDPRFHDSSSEEEEVEEDEEDGQEVKKEEQSSVEAKNTNSLFRPSEGPKLFCRTSQLAEQREQWEDRTSTLKQVSISSTKDYFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MT32</accession>
    <name>A0A3B5MT32_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Histone deacetylase</fullName>
            <ecNumber evidence="3 12">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3 12"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MT32"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027564"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027898.1">
        <property type="protein sequence ID" value="ENSXCOP00000027564.1"/>
        <property type="gene ID" value="ENSXCOG00000020570.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157440"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10162">
        <property type="entry name" value="ClassIIa_HDAC4_Gln-rich-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000002">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046949">
        <property type="entry name" value="HDAC4/5/7/9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024643">
        <property type="entry name" value="Hist_deacetylase_Gln_rich_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45364:SF13">
        <property type="entry name" value="HISTONE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45364">
        <property type="entry name" value="HISTONE DEACETYLASE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12203">
        <property type="entry name" value="HDAC4_Gln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037911">
        <property type="entry name" value="HDAC_II_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0678">Repressor</keyword>
    <keyword evidence="10 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Histone deacetylase glutamine rich N-terminal" evidence="19">
        <location>
            <begin position="62"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Histone deacetylase" evidence="18">
        <location>
            <begin position="657"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="203"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="413"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="486"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="604"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="56"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="204"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="254"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="426"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="607"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="779"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="649"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="651"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="657"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="727"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="site" description="Contributes to catalysis" evidence="15">
        <location>
            <position position="952"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR037911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037911-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037911-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR037911-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF12203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1061" mass="116907" checksum="192AA7219EE4143E" modified="2018-12-05" version="1">MSPLGKPGTRGGERREGRETPIRLRPEQGTTETEMDINSALPLRLPPAAIPMDLRVDHHHQQQQQLQQELLALKQKQQIQRQILIAEFQRQHEQLSRQHEAQLQEHIKQQQELLALKHQQELLEHQRKMENHRLEQELEKQQREQKLQLLKNKERGQESAVASTEVKMRLQEFVLNKKKALAQRSLNQGSLPNDAPYWYRKTQHSSLDQSSPPQTAVSTYNHPVLGVYNPRDDFPLRKTASEPNLKLRSRLKQKVSERRSSPLLRRRDSPITTAKKRSLDMADSACSSAPGSGPSSPNNSSNNIPNENGITVSISNNMEASLAQRLCGGGDQGSINQLSLYTSPSLPNITLGLPATATATAASNDAGLQPALSLSPPFLSGGHLSPYLADSGSGGHGAHSPLLQHMVLMEQSPAQSPLVTGEAESKSVSGMSMSSASMAKLQRQHRPLGRTQSAPLPQGSAAQAHAQALALQQLVVQQQHQQFLEKHKQQFQQQQLHLNKPGESPVGRQHQSHPEETEEELREHQDAGALPPGVTIKQEPPDPQELQELQELQEEALQQHREREQELLFRQQALLLEQQRIHQLRNYQASMEAAGMSMSFPGHRPLSRAQSSPASASSFPISVPTQDPPVKPRFTTGLVYDSLMQKHQCMCGNTNSHPEHAGRIQSIWSRLQETGLRAQCECIRGRKAALEELQTVHSEAHVLLYGTNPLRQKLDCSITPMFVRLPCGGVGVDSDTIWNEVHSSSAARLAVGSVVELVFKVATGELKNGFAVVRPPGHHAEESTPMGFCYFNSVAIAAKLLQQRLSVSKILIVDWDVHHGNGTQQAFYDDPNVLYMSIHRYDDGNFFPGSGAPDEVGSGPGVGFNVNVAFTGGLDPPMGDSEYLTAFRSVVMPIADEFAPDIVLVSSGFDAVEGHPPPLGGYTLTSKCFGYLTRQLMTLAGGRVVLALEGGHDLTAICDASEACVAALLGQELDPLPKAVLEQRPNPNAVRSLEKVIETHSKYWRSVHRYSSRLGLSLLEAKRGDSEEAEAVSAMASLSVFLTFFSPSRTEEEPMEEEAPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MTS5</accession>
    <name>A0A3B5MTS5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Interactor of little elongator complex ELL subunit 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MTS5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026901"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027230.1">
        <property type="protein sequence ID" value="ENSXCOP00000026901.1"/>
        <property type="gene ID" value="ENSXCOG00000020087.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006883"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045945">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019535">
        <property type="entry name" value="ICE2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14633">
        <property type="entry name" value="LITTLE ELONGATION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14633:SF3">
        <property type="entry name" value="LITTLE ELONGATION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10505">
        <property type="entry name" value="NARG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Little elongation complex subunit 2 C-terminal" evidence="2">
        <location>
            <begin position="634"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="491"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="852"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="378"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="505"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="525"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="855"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="865"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="886" mass="98681" checksum="2A1F18A89277D654" modified="2018-12-05" version="1">LPFNDRDDDAPPDGPFFTRDLYDKHSLAPHIRELWAFLQSAPSQTNPAKLKGSQAFGWESCDSDGTDSEEPKKSKRERTSCKKTETEIDVAYPEPRLPYPCLSSLSTKDQKTYVGYLMNMKSRTLPKVQNLILRINNEVIQFTKYLQDVAKICADDYNFITQGAMQYTEEFFTACLESVKALPQFYQIDEVTSLTGGTFNPGLALTFEKQLLTMMVPADAQLASDYHSVSSANPPAKKAKDKHRVSSDGNTEKLCAYYEPHVCLTRDALVRLLDNHGPDFGDQWELPVLVKITPGKGSSKRKTVYIDSPLLQRDVTIRERSHIYYEESLKLSIMTNGSKKVFHLMTERPAPEQQQVSQFKSSLNFEVDLTNLETFGETTTKTPQIKKTQSQQDEELQSSGSSSTPVVTKPKNSNKTLKVSSFSQGGLNSASKNKNNTRTETSQLIVSEPELQKTESSGSDQKCDKDEGFMAGSDDEKLIIDETGSSVALPTNQSERLTPTCSADSSVTSSVQSTPANTVSSPPCKRTRSKRTTRQTKAPKDQLGEILRMQTAMFKSGSEPSPAVKSPSRCLGPQLNPHTASMVKPCVSSYLERHQNEDAESRVVLPESVITHISPTEHKKILSENLQACAEDEQDYEAPVEGNLLYKLYSLQDLLVMVRSSVSLTHTRKHVPVHVLPKLEYQLSYGVECLTNSEACQLWTETSLHSSTVPYIAHINAHTSKVALLRKLPDDWMQNISCGFKPSKSLNILHHLLKKLTGLDKGQYLIVHKTGEAFVTLLKAGGEKVSRGAYNLQQNHSSVSQPPKLGIVPWIPVDPAVVLPFHQQHGRVPCTFPPNTFQKVSNQLNSHKKITIGGQKKNPNVQKNKQTKRSARRKKYIKNPSISCSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MTY9</accession>
    <name>A0A3B5MTY9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Ubiquitin carboxyl-terminal hydrolase 8</fullName>
            <ecNumber evidence="7">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Deubiquitinating enzyme 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Ubiquitin isopeptidase Y</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Ubiquitin thioesterase 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Ubiquitin-specific-processing protease 8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="21">Forms a ternary complex with RNF128 and OTUB1. Interacts (via C-terminal UCH catalytic domain) with OTUB1 isoform 1. Interacts with STAM2 (via SH3 domain). Interacts with DNAJB3, EGFR, EPS15, RASGRF1, RNF41, YWHAE, YWHAG and YWHAZ. Interacts with NBR1, RASGRF1, RNF41 and IST1. Associates with the ESCRT-0 complex and with microtubules. Interacts with BIRC6/bruce and KIF23/MKLP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endosome membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MTY9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024702"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025001.1">
        <property type="protein sequence ID" value="ENSXCOP00000024702.1"/>
        <property type="gene ID" value="ENSXCOG00000018453.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157542"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000025">
        <property type="entry name" value="Putative ubiquitin carboxyl-terminal hydrolase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000011">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000017">
        <property type="entry name" value="ubiquitin carboxyl-terminal hydrolase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015063">
        <property type="entry name" value="USP8_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048498">
        <property type="entry name" value="WW_USP8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF27">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08969">
        <property type="entry name" value="USP8_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20625">
        <property type="entry name" value="WW_USP8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140856">
        <property type="entry name" value="USP8 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0645">Protease</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0729">SH3-binding</keyword>
    <keyword evidence="15" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="13" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="28">
        <location>
            <begin position="687"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="122"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="321"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="571"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="455"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="530"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="27">
        <location>
            <begin position="582"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="597"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="617"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="635"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00063318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00070641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00076185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00077311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00078426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00082456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1019" mass="116279" checksum="7F431C5332B95B21" modified="2018-12-05" version="1">MPAVSTGAKELYLSTSLGDLNKKAEIKPDKTSTRSYVQSACKLFKAAEECRLDRDEEKAYVLYMKYLTVYDIIKKRPEFKQQPDYYMTMLGANSFKKAIEEAEKLSESLKLRYEEVEVRRKLEEKERLEEKKKREEVTEKDGRNSPKASTINKKDSKKVNKSTTQHFASETGGITAEKLFNMMKDQTISVLVMDARSHRDYEESHMQVPAQTCISVPEEAVSPGITVTLIEAKLPDASKDHWRRRGVVDYVVLLDWFSSASDLKLGTTLMSVKDAIYKWDSLTIMRSEPLVLEGGYENWLLFYPMYTTNAKVRPPRQTITNSLPQLNFSYPSLEEPKPPTPTPPEREISPEPQEPAAPALVNGVSPAEPPASKTSVVADRLPDTVDLPITYDRAKKPSVPVSDQTKSGQNRSAIDSTQNGPIIPDRSAKPPLSSGGSLSKDEQQQIHSEAVAVMEKAKQEQEKRNQERRLEEERRENEQKEREESEKRKKEEEEKRHQEKKRLERQTAEEEEDKEKKPLCECLFGSQREPLTRARSEEMGRSVPGLPDGWMKFLDTVTGTYRYYHSPTNRVHLYPPEVTVPQTPPSTPPTAKPRSQKPAEPDTSHDREREQSKLKRSYSSPDISQEVSEEAQKKTTAPTAAAVIPTINRETKPPSVKVYSKVESVRPSAAKLRNLNPSFGGLGASLTGLRNLGNTCYMNSILQCLCNTPRMADYFNNNLYLEDINRSNILGHKGEVAEEFGVIMKALWAGLYKCISPRDFKSTIGKINDQFAGYDQQDSQELLLFLMDGLHEDLNKADNRKRYKEEENDHLDDQNAANVAWNKHKLLNESIIVALFQGQFKSTVQCLTCHRKSRTFETFMYLSLPLASTSKCSLQDCLRLFSKEEKLTDNNKVFCRHCKAHRDSTKKLEIWKVPPIVLVHLKRFSYEGRWRQKLQTSVDFPLDSLDLAQYVIGPKQNLRRYNLYAVSNHYGGLDGGHYTAYCKNSLKQRWYKFDDHEVTEISSSSVKSSAAYILFYSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUH3</accession>
    <name>A0A3B5MUH3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pyroglutamyl-peptidase I like</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C15 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MUH3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027156"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027489.1">
        <property type="protein sequence ID" value="ENSXCOP00000027156.1"/>
        <property type="gene ID" value="ENSXCOG00000020273.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015368"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.20">
        <property type="entry name" value="Peptidase C15, pyroglutamyl peptidase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016125">
        <property type="entry name" value="Peptidase_C15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036440">
        <property type="entry name" value="Peptidase_C15-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402">
        <property type="entry name" value="PROTEASE FAMILY C15 PYROGLUTAMYL-PEPTIDASE I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402:SF1">
        <property type="entry name" value="PYROGLUTAMYL-PEPTIDASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01470">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53182">
        <property type="entry name" value="Pyrrolidone carboxyl peptidase (pyroglutamate aminopeptidase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="196" mass="21789" checksum="FA72B8806BB4F80B" modified="2018-12-05" version="1">MSSSSRRFGPFRQFLVNPSWTTAQGLKLAGMGQRIDVYIKELPVSYSSTQRIIAELWQTLKPKFAVPLGIARGSSLVILEQTGKNSGYSTRDVCNCSPTDHLCIVGGPEKLDSVVNMRTISKHFKQAGMDVVHSRDAGRYLCDFAYYCSLYHGERRAAFIHIPSSGSLSSAERLVPLLQETIVMMLDQLEEIHCGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXF4</accession>
    <name>A0A3B5MXF4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Efficient DNA binding requires dimerization with another bHLH protein.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXF4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026636.1">
        <property type="protein sequence ID" value="ENSXCOP00000026317.1"/>
        <property type="gene ID" value="ENSXCOG00000019651.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160217"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043010">
        <property type="term" value="P:camera-type eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11427">
        <property type="entry name" value="bHLH_TS_NeuroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000006">
        <property type="entry name" value="Neurogenic differentiation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022575">
        <property type="entry name" value="NeuroD_DUF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF86">
        <property type="entry name" value="NEUROGENIC DIFFERENTIATION FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12533">
        <property type="entry name" value="Neuro_bHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="10">
        <location>
            <begin position="128"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="92"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="307" mass="33583" checksum="6EA43975ECB0AA44" modified="2018-12-05" version="1">MWPFWSEQPLRERNELVRQSISALHWDGWSKHLLTRNFKLSHTVRLRLLSASPLRSMRPRCGFSGRPRKATKAWASPVCLSAQPRRAFAQLPKTQAHKPTHACTTSGAPEGRVGDGGALSHGPDTRDLQRIKANARERSRMHGLNDALENLRSIMPCHSKTQKLSKIETLRLARNYICALSEALEAGLSTESRAFMETLCKGLSQPTTNLVAGCLQIGSGPGAGMRPEDRLGVCPPPNPLGGMVGYTSPGLPSPPYGTYDTAHMLHLRAMKGRDISLSSHFSPQLCGLAALIPGLSLNEAWMREAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZM0</accession>
    <name>A0A3B5MZM0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fc receptor, IgE, high affinity I, gamma polypeptide like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MZM0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026050"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026363.1">
        <property type="protein sequence ID" value="ENSXCOP00000026050.1"/>
        <property type="gene ID" value="ENSXCOG00000019462.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000174764"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032998">
        <property type="term" value="C:Fc-epsilon receptor I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019767">
        <property type="term" value="F:IgE receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021663">
        <property type="entry name" value="CD3_zeta/IgE_Fc_rcpt_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042340">
        <property type="entry name" value="FCER1G"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16803">
        <property type="entry name" value="HIGH AFFINITY IMMUNOGLOBULIN EPSILON RECEPTOR GAMMA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16803:SF0">
        <property type="entry name" value="HIGH AFFINITY IMMUNOGLOBULIN EPSILON RECEPTOR SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11628">
        <property type="entry name" value="TCR_zetazeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="79" mass="9113" checksum="B4C95BD93D1CDA68" modified="2018-12-05" version="1">MFVYSLMMFVCVEAAGDMNVCYILDGVLILYGIILTVLYCRLRVRPIKNELSFHAGIIVLFKNITDTYETIKMDKKPIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N0N9</accession>
    <name>A0A3B5N0N9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0415 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N0N9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026780"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027109.1">
        <property type="protein sequence ID" value="ENSXCOP00000026780.1"/>
        <property type="gene ID" value="ENSXCOG00000020003.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014722"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010733">
        <property type="entry name" value="DUF1308"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041076">
        <property type="entry name" value="DUF5614"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13379">
        <property type="entry name" value="UNCHARACTERIZED DUF1308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13379:SF0">
        <property type="entry name" value="UPF0415 PROTEIN C7ORF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07000">
        <property type="entry name" value="DUF1308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18474">
        <property type="entry name" value="DUF5614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5614" evidence="4">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1308" evidence="3">
        <location>
            <begin position="252"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="193"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="194"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="415" mass="46275" checksum="D771959B1712840C" modified="2018-12-05" version="1">MSLQATLRDRISSAQALLQRAEHLCPGVEGHQKLCGKLRAELRFLRRVEAGELQVKESHLHSTNLTHLTAIVDSAQDLEDVVALLHVFSYRDVAGRRRTLVVDVVADAGLTWVKAVGRKAEALHNIWQGRGQYGDKSVVRQAEDFLLASRQLPVNYRHPRVVFAFYNGLSSPMADRLRHLGVAVRGDIVAVRVEEEEEEEEKDEEEVEEEEADPDDPEGELASGLTRVQRGTVLARLAFPLPPPPPEECGRVNLDITTLIAYVSALSHGRCHFSFREPVLTEQAAQERRWPVLPSLDAFMAGKQLFACRAAVADFRAILDTLGGPGEKERAQTLLARLHLVDDQPSERSRRLTPSAKVNRRSLAIFGTGDFLRAVTMTANSRFVRAAANQGVRYSVFIHQPRALTEGKEWRATPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N1G0</accession>
    <name>A0A3B5N1G0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FBXO31 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N1G0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027475"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027808.1">
        <property type="protein sequence ID" value="ENSXCOP00000027475.1"/>
        <property type="gene ID" value="ENSXCOG00000020513.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001368"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22102">
        <property type="entry name" value="F-box_FBXO31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000042">
        <property type="entry name" value="F-box protein 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045048">
        <property type="entry name" value="FBXO31/39"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10706">
        <property type="entry name" value="F-BOX FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10706:SF130">
        <property type="entry name" value="F-BOX ONLY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12014">
        <property type="entry name" value="Cyclin_D1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="52"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="13"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="515" mass="58575" checksum="9D8823E9DF0BCB61" modified="2018-12-05" version="1">MAVCARLCGVGQSRRCRRRQRNDQQEQGSDSDMDEEEEEKIVGGSNTGPPHPESLMELPPELLVEIFSLLPGTVLPNVALVCKKFRQILNTETIWRRRCAEEFGMREDLRKMEATGVSSQDLYVKLLHPYRHILGLWQPDIGPYGGLLNVVVDGLFIIGWMYLPPHDPRVEDPMRRRPLFRIHLLESKKATVECMYGHKGPHKGDIQTVKKDEFSTKCNQTDHHRMPGGRQEEFRTWLEEEWGRTLEEIFHEHMQELILMKFIYTSQYDNCLTYRRIYLPPLMPSDLLQPGLFKGTYGSHGLEIVMLSFHEMSARATKLTGDPNVPAGQLTLDVDLSQPVDLPELDQQRNIDVLSRIILGIHEKVQREVEQPASDTSACVEHAEEEADDAAAEETEVDHGACVDACQSGPSGSSSSPEAQPFVLPLGVMARNEVYPRTCRKCFYGTGLIAGHGFTSPERTPGLFVLFDEDRFGFIWLELKSFSLYSRLMDHLPHAHAPSMEPFEAMLRNMQSWTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N1R9</accession>
    <name>A0A3B5N1R9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5N1R9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028075.1">
        <property type="protein sequence ID" value="ENSXCOP00000027740.1"/>
        <property type="gene ID" value="ENSXCOG00000020675.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160472"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032483">
        <property type="term" value="P:regulation of Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000042">
        <property type="entry name" value="C-myc promoter-binding protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051696">
        <property type="entry name" value="DENN_Domain_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296:SF18">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03455">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13041">
        <property type="entry name" value="PPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00801">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="4">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="401"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="310"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="543"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="655"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="804"/>
            <end position="850"/>
        </location>
    </feature>
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        <location>
            <begin position="1055"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="816"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="828"/>
            <end position="842"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1081" mass="121262" checksum="EC76F0FD07BEBB96" modified="2018-12-05" version="1">PLTVIFESLHAVQHGVLQAGDTCFSLPADVWLSLCVQMTFPLRWLCPYIPLCPLQMADVLLAPMPFIVGVHSSYFDLYDPPPEVVCVDLDTNTVFQSEDKKPLSWRSLPRKHGKTLFVTLTNLHRTLEKSECPGQEEATLEFLLTDYDQIYRRQKQLELEIQEAFLRFMSCLLRGYRAFLLPITQAPSDTTTDCSSLFNLQGFLKSRDRSQQKLYAQLSRTQMFTQFIEECSFVSDRHACLEFFDECVQKVDVEKPEEVRLIDLDENGGGEHTVFIMPPEEPQEADGAECPARYSYETFPRLTAELFDRPQDPLRAPTRGSAPSSPAPRRTKQEMKVAQKRAQKYSAVPDMWSKCLLGHCYGLWFIYLPTFVRAEGSKVRALHSAHDVLRHMETRKVVLPDEVCYRILMQLCGQYGQPVLAVRVLLEMKKAGITPNTITYGYYNKAVLECKWPSTNQGGRLRWAKLRNVLLAVAQFRQPIKRRRKSGSVGSRGQLSRSSSCRINPRLQSALIRQSSWSGLSESSSHESLTGSLVKSSSLSSMKTPTVSVCKKSPVLHGSGTNGDTVHRKPPLGRREASAPPAAPPEGVVVRRSQVCLSTFYTESELDCRCEPRLSRSARTVDENSNHVSSPSRGGLAGKLQQLLTPTKNRASVRRAASVENRWSGGGIGRKVSDQRQSRKSQGPESLLKAKERLVNATSESSLSVGSDLDLSDTPTTVYPLRKSWDANQEGAGIEVLMSSCSLCRSCNSLVYDEEIMAGWTSDDSNLNSSCPFCAASFVPFLNAEICDPGPVSSAERCTMNLEDEVESAVRPPSSHEASQRPQCNGLSDDSSSETSTYSETSKATTVSSVGGTPQVTVAYLSPLVLRKELESLLENEGESVLAQPQFLDSHSIIFWNLVWYFQRLGLPSNLLQLVRSSPLVSHFTQSENSAVRIRLLWDTLSPDTDQWPPLYVLWRIHSGAPMRSYSWRRHNHPFTLSFLEEVLRWVGMNEVHKAITLFLDTLEKQPGSPQTQRSLYREMLFLTLAAMGKDHVAAFDKKYKTAFLRLSSSLGRDQLRRKRAQPPSTKAVDCRRSFHPPLEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N1S5</accession>
    <name>A0A3B5N1S5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cholesteryl ester transfer protein, plasma</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N1S5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027755"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028090.1">
        <property type="protein sequence ID" value="ENSXCOP00000027755.1"/>
        <property type="gene ID" value="ENSXCOG00000020695.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000263546"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034364">
        <property type="term" value="C:high-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120020">
        <property type="term" value="F:cholesterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005548">
        <property type="term" value="F:phospholipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017129">
        <property type="term" value="F:triglyceride binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034375">
        <property type="term" value="P:high-density lipoprotein particle remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034374">
        <property type="term" value="P:low-density lipoprotein particle remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046470">
        <property type="term" value="P:phosphatidylcholine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055091">
        <property type="term" value="P:phospholipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043691">
        <property type="term" value="P:reverse cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070328">
        <property type="term" value="P:triglyceride homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034197">
        <property type="term" value="P:triglyceride transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034372">
        <property type="term" value="P:very-low-density lipoprotein particle remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.20.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017943">
        <property type="entry name" value="Bactericidal_perm-incr_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017130">
        <property type="entry name" value="Cholesteryl_ester_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017942">
        <property type="entry name" value="Lipid-bd_serum_glycop_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47616">
        <property type="entry name" value="CHOLESTERYL ESTER TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47616:SF1">
        <property type="entry name" value="CHOLESTERYL ESTER TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01273">
        <property type="entry name" value="LBP_BPI_CETP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037185">
        <property type="entry name" value="Cholesteryl_ester_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00328">
        <property type="entry name" value="BPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55394">
        <property type="entry name" value="Bactericidal permeability-increasing protein, BPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017391160" evidence="6">
        <location>
            <begin position="21"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Lipid-binding serum glycoprotein N-terminal" evidence="7">
        <location>
            <begin position="37"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="165"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR037185-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="417" mass="46623" checksum="3B93FDAEC677D724" modified="2018-12-05" version="1">MPGEISPWLLLLSLFGTTHSCLQDPSSAYKYTGIVCRLTYPAAVVLNEKTTKVIEAAFQHARYPSMQGEKSAGFFGKVSYGLENLEIYNLSIGRSDFELRPGEGIGLEINNVSAVFSGTIQYGYGSWLVNVGHRLDFEVETQIDLGINPKLYCGNSKVAADTSDCYLNFHKFRLYLEGDKEPNWLKKLFTDFITFTVKLVVKGQICKEINKLADILAEFIQDTAANFLSDGGISVDIGVTTTPLITVNYIESYHKGLTTYLNLTSVINTSVFHPDHLTENRMLYFWFSDVALCLAAQCLLESASPELRVWSSSVPTLNTSTVGTSVWAQATGELYCGSQNKPTLSFQMVHRGILQLRNVLCFPVLEVEITRLMDKQGANLFDIIDPEVLHQEGYIVTQMDFGFPHHLLVDFLKRTLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KJV4</accession>
    <name>A0A3B5KJV4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GDNF/GAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GDNFR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KJV4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000448"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000456.1">
        <property type="protein sequence ID" value="ENSXCOP00000000448.1"/>
        <property type="gene ID" value="ENSXCOG00000000388.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165282"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.110:FF:000001">
        <property type="entry name" value="GDNF family receptor alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.110">
        <property type="entry name" value="GDNF binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016017">
        <property type="entry name" value="GDNF/GAS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037193">
        <property type="entry name" value="GDNF_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003438">
        <property type="entry name" value="GDNF_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269:SF15">
        <property type="entry name" value="GDNF FAMILY RECEPTOR ALPHA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269">
        <property type="entry name" value="GDNF RECEPTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02351">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01316">
        <property type="entry name" value="GDNFRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00907">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110035">
        <property type="entry name" value="GDNF receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GDNF/GAS1" evidence="10">
        <location>
            <begin position="44"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="10">
        <location>
            <begin position="136"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="327" mass="37383" checksum="56ABD8CE807D4A0B" modified="2018-12-05" version="1">APKHIHLLPEREDDRAFPSSLFLHIPLSAFSPKPFSFLDGENQCLKAAQDCGLYEKCGALRSEYVLACTKPMPNTSRCNQYKCHRALRRFLERVPEEYSLGVLFCPCTNTLCGERRRKTIVPSCSYQDAESLQPNCLHQQSFCRRDDLCRSRLADFLSNCQPSHLTASGCLKDSTGLCLRAYAGLIGTIMTPNYISNNSADVSLWCNCEGSGNQWQECLRLQRLFTHNSCLREFALCADWCSLFVPDSRYLSRQRSLNGVILEEYRSCKGQTLQSITFLAVHEIRCNLCLSLVVAPLYHMVIEEVRMDLMMIKLHHQLSWQFEFLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KNN4</accession>
    <name>A0A3B5KNN4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NSFL1 cofactor p47</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">p97 cofactor p47</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">Golgi stack</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027875824.1">
        <property type="nucleotide sequence ID" value="XM_028020023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KNN4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000082"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000082.1">
        <property type="protein sequence ID" value="ENSXCOP00000000082.1"/>
        <property type="gene ID" value="ENSXCOG00000000067.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114146171"/>
    <dbReference type="CTD" id="55968"/>
    <dbReference type="GeneTree" id="ENSGT00520000055567"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990730">
        <property type="term" value="C:VCP-NSFL1C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061025">
        <property type="term" value="P:membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031468">
        <property type="term" value="P:nuclear membrane reassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14348">
        <property type="entry name" value="UBA_p47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000020">
        <property type="entry name" value="NSFL1 (p97) cofactor (p47)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000093">
        <property type="entry name" value="NSFL1 (P97) cofactor (P47)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.210:FF:000001">
        <property type="entry name" value="NSFL1 (P97) cofactor (P47)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.210">
        <property type="entry name" value="SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036241">
        <property type="entry name" value="NSFL1C_SEP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012989">
        <property type="entry name" value="SEP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333:SF24">
        <property type="entry name" value="NSFL1 COFACTOR P47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23333">
        <property type="entry name" value="UBX DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08059">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00553">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102848">
        <property type="entry name" value="NSFL1 (p97 ATPase) cofactor p47, SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51399">
        <property type="entry name" value="SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEP" evidence="13">
        <location>
            <begin position="180"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="UBX" evidence="12">
        <location>
            <begin position="294"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="44"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="263"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="57"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="272"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="373" mass="40851" checksum="47B2D483B3C9EF03" modified="2018-12-05" version="1">MASQEESVREFVAVTGVEEERARFFLESAGWNLQLALGSFFEDGNDDDIITLPQPEGGSSVSRSTGPSQPRVTSFRDLMHEAEDESDEEEGQRFFAGGSERSGQQIVGPPKKKSSNEVVEDLFKGAREHGAVPLDRTGKGLGESSKAKAFVGGGYRLGAAPEEESTYVSGERQSSNSQQDVHVVLKLWKTGFSLDNGELRTYNDPGNANFLEAIRRGEIPLELRQRARGGQVNLDMEDHRDEDFAKPKVSFKAFEGEGQKLGSATPELISAPPTSQQDQAANEAQASSSVSLDPSQPTTNIQIRLADGGKLVQRFNHTHRVSDLRQFVVLARPAMAAREFVLMTTFPNKELADESQTLQEANLLNAVIVQRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KNQ9</accession>
    <name>A0A3B5KNQ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KNQ9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001768"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001794.1">
        <property type="protein sequence ID" value="ENSXCOP00000001768.1"/>
        <property type="gene ID" value="ENSXCOG00000001412.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153739"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01371">
        <property type="entry name" value="KISc_KIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000017">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF76">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 9 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="12">
        <location>
            <begin position="9"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="484"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="96"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="720" mass="82507" checksum="86194695FACDA393" modified="2018-12-05" version="1">MSAGKQCETVRVVVRCRPFSRREETAGCENILEVDDRLGQITIRNPKVPPDEPMKVFTFDSVYGWNSKQRDIYDDAVRPLVESVLHGFNGTIFAYGQTGTGKTHTMQGASKDPEKRGVIPNSFEHIFTQISRSQNQKYLVRSSYLEIYQEEIRDLLCKNNNKKLELKENPDCGVYVKDLSSVVTKNTTEIEHVMNIGNQSRSVGFTNMNERSSRSHAIFLVTVECSERGPDGEDHIRVGKLNMVDLAGSERQSKTGAKGKRLKEAAKINLSLSALGNVISALVDGKSTHVPYRDSKLTRLLQDSLGGNAKTVMIATVGPTHKSFDETLATLRYASRAKRIKNKPRINEDPKDALLREFQEEIARLKAQLEERGMLAKERRRRRRDSKRLSKSLAGMEEDILCEKDGNVWKAAEEAEEDGRHCLIEGGDSAKILTCQGGTEKLKNLNLYRRSLGDMQWDQDAVEKIIEKYKAMESKLLVGGKTIIDHTNEQQKILEQKRQEIAEQIRREREIQQQMMLQDEETLEIRETFSSLQQEVEHKTKKLKRLHAKLQLLRGEMKDIFDEHVTTRQELEETQEELTRELKYKYLLIENFIPPEEKNKIMNRLQFDCEEDQWWLQPVIPSESTPTQVKRRPLSAVGYKRPISQYVQMAVATTTGIPFRYQAENVMLLELDMSPPTMFTLNLNGECLERAFTPRLLPDLSLNVRARGASSSRVRKSQSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KR67</accession>
    <name>A0A3B5KR67_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Glycine receptor, alpha 4a</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KR67"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000131"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000132.1">
        <property type="protein sequence ID" value="ENSXCOP00000000131.1"/>
        <property type="gene ID" value="ENSXCOG00000000120.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158789"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016934">
        <property type="term" value="F:extracellularly glycine-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045664">
        <property type="term" value="P:regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043200">
        <property type="term" value="P:response to amino acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001964">
        <property type="term" value="P:startle response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001966">
        <property type="term" value="P:thigmotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd19060">
        <property type="entry name" value="LGIC_TM_GlyR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000003">
        <property type="entry name" value="Glycine receptor alpha 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0868">Chloride</keyword>
    <keyword evidence="10" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="12 13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="350"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="14">
        <location>
            <begin position="89"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="15">
        <location>
            <begin position="188"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="383" mass="44222" checksum="5444A254A09ADE6B" modified="2018-12-05" version="1">HTNSVRFASFIDLASTVSSGEMPLGPGQFESLYKLLDSGLKQFRCTFVLIKLTVISICNLTMSPYVWNHFSLRPSYIPYGSRSFFPICFCLRLTLILSCPMDLKNFPMDIQTCTMQLESFGYTMNDLIFEWLSDNPVQVADDLTLPQFVLKEEKDLGYCTKHYNTGKFTCIEVKFHLERQMGYYLIQMYIPSLLIVILSWVSFWINMDAAPARVGLGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFAALLEYAAVNFVSRQHKEFIRLRKKQRQQRIEEELMRESRGFYFRGYGLGHYLQAKDGTAVEGPTVFSRLPPVTIYDGEVLHKRFVDRAKRIDTISRAVFPLSFLIFNIFYWVTYKVLRHEDIHANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRU3</accession>
    <name>A0A3B5KRU3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCR4-NOT transcription complex subunit 10</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the CNOT10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRU3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002828"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002865.1">
        <property type="protein sequence ID" value="ENSXCOP00000002828.1"/>
        <property type="gene ID" value="ENSXCOG00000002247.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001827"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039740">
        <property type="entry name" value="CNOT10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12979">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12979:SF5">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="417"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="647"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="425"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="662"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="676" mass="74763" checksum="A5DF6E7265623A01" modified="2018-12-05" version="1">MTDQEKEMATSAYEAFLAGKYDESLKQLEALQELNKEDYKISLNKAVLEFYRSGQTATGSLKQTLSAMKNQLHTATEDVDGLDDVENSLLYYNQAIIHYHMRQYSEAISIGEKLYQFLEPFEEKFAQSVCFLLVDLYLLTFQPEKALHLLAILDKIAVQGSNKNGKAESNNSGSNQKVEFVAMMEAAKSKIHQSAPSLFLKSNFEYQRGNYRKAVKLLNSSNMAEHPGPIRTGECVRCMFWNNLGCIHFAMGKHNLGIFYFKKALQENDHTCAQLGDGKRLSGVPMCALLFNKRYELLYNCGVQLLHAARPLAAFECLMEAVQVYHSNPRLWLRLAECCISANKGGSEQESKSLPSKKGIVQSVIGQGYHRKIVLASQSSQNTNYSEGQSAAIPVASMEFAAICLRNALLLLPEHQQPEAKAESGSKNSSQSGSTESGSENSDPCSGKSQEADKFFSATPSSPLRKQEVENLRCSILACSAYVALALGDNLLALHHADKLLHQIKVSGSLRFLGHLYAAEALISLDRISDAITHLNPENISDVSLGVLSSEQDQGDEPIEPSAKKMPLFYPSSVAAAQAMMLFNLGSAYCLRSEYDKARKCLHQAASVMNSKEIPPEAILLGVYLELQNGNTQLALQIIKRNQLLPSTLNRSSPDPRKKPSQPLQLPSSFTHVQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KSD7</accession>
    <name>A0A3B5KSD7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027894284.1">
        <property type="nucleotide sequence ID" value="XM_028038483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KSD7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000845"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000855.1">
        <property type="protein sequence ID" value="ENSXCOP00000000845.1"/>
        <property type="gene ID" value="ENSXCOG00000000709.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114157477"/>
    <dbReference type="KEGG" id="xco:114157477"/>
    <dbReference type="GeneTree" id="ENSGT00510000050415"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="21"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="271" mass="30843" checksum="3875A9ABBF3771A0" modified="2018-12-05" version="1">MSIQEQDSPEKKYDPLDKTLKFVNRGDDLDPVSSDFNSLRAEMSCGHAVTPDSLTQWCRIQLDEGNHKFRCPAVVEGTKLCNKLWSYQEVRRLADLSVDEMEYFEQKMASLSVSEHCEVQSCPKCKTTVERKDLSNLCVQCVICTADQKKTYQFCWQCQKEWKGSGPRSDRCSNDGCINRDLQLLQTCKDISLPEVEGVTSCPSVRACPVCGMKVEHNRMYCKNVTCPRCRLTFCFVCLKPKSECCQSSSPYRICPGGVAPRQSRIPVWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KSK8</accession>
    <name>A0A3B5KSK8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KSK8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000920"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000931.1">
        <property type="protein sequence ID" value="ENSXCOP00000000920.1"/>
        <property type="gene ID" value="ENSXCOG00000000770.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000002"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001540">
        <property type="term" value="F:amyloid-beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01272">
        <property type="entry name" value="PTB1_Fe65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01271">
        <property type="entry name" value="PTB2_Fe65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000019">
        <property type="entry name" value="Amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000003">
        <property type="entry name" value="amyloid beta A4 precursor protein-binding family B member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000034">
        <property type="entry name" value="amyloid beta A4 precursor protein-binding family B member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039576">
        <property type="entry name" value="APBB1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14058">
        <property type="entry name" value="AMYLOID BETA A4 PRECURSOR PROTEIN-BINDING FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14058:SF11">
        <property type="entry name" value="AMYLOID BETA PRECURSOR PROTEIN BINDING FAMILY B MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017313370" evidence="4">
        <location>
            <begin position="17"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="6">
        <location>
            <begin position="253"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="5">
        <location>
            <begin position="366"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="5">
        <location>
            <begin position="536"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="81"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="164"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="663"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="185"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="706" mass="76971" checksum="D028C0550A59BF8B" modified="2018-12-05" version="1">MLSNVLISSLFSIVGPLSRVTMMSVDVTNCNGPAATPPTSLSLRSSHNQLLSSDVIKQGSATPPKCRKKYALTSIQAAMGLGENAPPSTSPSLSPSQPAAPNNPKLAKNGANQLRKAGQDHNKNTTDPDPTDPECNPEPIEGRVSSGSEIISELQSNLALLQSEKNSGVPPPPRQDSPEDTRLLSAASCPSSSSSSPETKKDRRTGAKTDCALNRIQNLNPSDEELSWTTLSQDSNSPEETDIWSEQSFQTDPDLPPGWKKITDMAGIYYWHIPTGTTQWERPSTRPPPPGQNETPALTDPTAPVPRKIDSEVWKDLQAATVNPDPSLKEFEGATLRYASLKLRNRPTTEENESSSVNSDPEAKCFAVRSLGWVEMAEEDLAPGKSSVAVNNCIRQLSYCKNDIRDTVGIWGEGKDMYLVLENNMLNLVDPMDRSVLHSQPIASIRVWGVGRDNGRDFAYVARDKNTRILKCHVFRCDTPAKAIATSLHEICSRIMTERKNAKAMAGGSLQDRMQAGLDLPLQAEFPTPKTELVQKFQVLYLGMMPVARPIGMDILNGAIESLIGSSNREDWTPVALNVADATVIVISDQDEKEVLVECRVRFLSFMGVGRDVHSFAFIMDAGGHRFDCHVFWCEPNAGSVSEAVQAACMLRYQKCLVARPPSQKACGSSPPGDSVSRRVSTSVKRGVLSLIDTLKQKRPVTELPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KT64</accession>
    <name>A0A3B5KT64_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Amyloid beta (A4) precursor-like protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the APP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KT64"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001687"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001712.1">
        <property type="protein sequence ID" value="ENSXCOP00000001687.1"/>
        <property type="gene ID" value="ENSXCOG00000001364.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063252"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031694">
        <property type="term" value="F:alpha-2A adrenergic receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031695">
        <property type="term" value="F:alpha-2B adrenergic receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031696">
        <property type="term" value="F:alpha-2C adrenergic receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21708">
        <property type="entry name" value="JMTM_APLP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.770:FF:000001">
        <property type="entry name" value="Amyloid beta A4 protein-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.770">
        <property type="entry name" value="Amyloid precursor protein, E2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.140">
        <property type="entry name" value="Amyloidogenic glycoprotein, copper-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.570.10">
        <property type="entry name" value="Amyloidogenic glycoprotein, heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036669">
        <property type="entry name" value="Amyloid_Cu-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008155">
        <property type="entry name" value="Amyloid_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011178">
        <property type="entry name" value="Amyloid_glyco_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024329">
        <property type="entry name" value="Amyloid_glyco_E2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008154">
        <property type="entry name" value="Amyloid_glyco_extra"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015849">
        <property type="entry name" value="Amyloid_glyco_heparin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036454">
        <property type="entry name" value="Amyloid_glyco_heparin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019745">
        <property type="entry name" value="Amyloid_glyco_intracell_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019543">
        <property type="entry name" value="APP_amyloid_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019744">
        <property type="entry name" value="APP_CUBD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036176">
        <property type="entry name" value="E2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103">
        <property type="entry name" value="ALZHEIMER'S DISEASE BETA-AMYLOID RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103:SF13">
        <property type="entry name" value="AMYLOID BETA PRECURSOR LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10515">
        <property type="entry name" value="APP_amyloid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12924">
        <property type="entry name" value="APP_Cu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12925">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02177">
        <property type="entry name" value="APP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00203">
        <property type="entry name" value="AMYLOIDA4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00006">
        <property type="entry name" value="A4_EXTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56491">
        <property type="entry name" value="A heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89811">
        <property type="entry name" value="Amyloid beta a4 protein copper binding domain (domain 2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109843">
        <property type="entry name" value="CAPPD, an extracellular domain of amyloid beta A4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00319">
        <property type="entry name" value="APP_CUBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51869">
        <property type="entry name" value="APP_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51870">
        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00320">
        <property type="entry name" value="APP_INTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="8 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017243450" evidence="13">
        <location>
            <begin position="21"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="626"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="E1" evidence="14">
        <location>
            <begin position="30"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="E2" evidence="15">
        <location>
            <begin position="329"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="GFLD subdomain" evidence="10">
        <location>
            <begin position="30"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="CuBD subdomain" evidence="10">
        <location>
            <begin position="129"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="188"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="538"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="230"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="549"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="100"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="142"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="694" mass="79812" checksum="E4B2F065D253BC36" modified="2018-12-05" version="1">MSASALVLTLLFLSALQALAMTEVNGPGPQLAEPQIAMFCGRQLLHMNLQTEQWEPDPQGRQGCFKEPNEILSYCQEMYPALPISHIEESKRPVTIQAWCKKGLGHCQIVLPYRCLEGEYVSEALLVPDRCRFLHQEQMNICESYVYWHNIAKEECTAENLELHSYGMLLPCGDHFRGVEYVCCPGRSSSNGKGETEEKDVPQTLASQTVGKLNSVAKVMVPTPSPSPETDLDEVDMEEDDDEVIDEEEEEEEEEDDVNEDEAEEEEEEEEDETAAVKDREEYEYPFELGPYQTSDYLDSFYNDKSHKSTTSAPLARGDSFTTTRPTDGVDVYFEKPVDDTEHANFLRAKTDLEERRMKRINEIMKEWAEADNQSKNLPKSERQALNEHFQSVLQTLEEQVAGERQRLVETHLARVEAILNNNRRLALENYLTAVQADPPQPERVLQALKRYMAAEQKDRRHTLRHYQHIVAVDPQKAEQMKFQVYTHLHVIEERMNQSLALLYKDPTLAEDLHSDIQELVKAERGDISELMTTSFSETRTTEEFVPAESEEEKDDEEEEERAFQNRPYPPRIEDEYDYATSERGPTYEYEEKINTSAELKQVVKPNEIARDELQPDALETFNRGAMVGLLVVAVAIAMVMVISLLLVRRKPYGTISHGIVEVDPMLTPEERQLNKMQNHGYENPTYKFFEQMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTC7</accession>
    <name>A0A3B5KTC7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Translocase of outer mitochondrial membrane 40 homolog, like</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom40 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTC7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001757"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001783.1">
        <property type="protein sequence ID" value="ENSXCOP00000001757.1"/>
        <property type="gene ID" value="ENSXCOG00000001420.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003308"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07305">
        <property type="entry name" value="Porin3_Tom40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037930">
        <property type="entry name" value="Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10802">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="308" mass="33304" checksum="0977E4ED03AABB37" modified="2018-12-05" version="1">MGSVLAASAAPAPPLPPGFGLPPLPPPATGSGPQREAKNVLPNPGAFEQCHQKVFPTQVNGVRLVLNKALSKHFQLSSTEDSGYRFGTVFVGSGQAGPAEFYPVLVGDVDSSGSLNAQVIHRVTDRIRSKVVFQQTHQQTFVTWKADTEFRGEDFTAAVAFTNPDVLDDSHYLQSVTPALAMGGHLVYRRRPGDEASFLSLVGRYTSNFVGALTLGWASVQASYYHRASDKLQLGVELETGAFLEDSSVTFGYQLDVPEANLQFKVINSNWVVGATLEKKLLPLPLSLVLCSFLNHQKNKFHCGFGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KVE3</accession>
    <name>A0A3B5KVE3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AHNAK nucleoprotein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KVE3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002492"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002526.1">
        <property type="protein sequence ID" value="ENSXCOP00000002492.1"/>
        <property type="gene ID" value="ENSXCOG00000001989.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154902"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052082">
        <property type="entry name" value="Myelin_sheath_structural"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23348:SF41">
        <property type="entry name" value="NEUROBLAST DIFFERENTIATION-ASSOCIATED PROTEIN AHNAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23348">
        <property type="entry name" value="PERIAXIN/AHNAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="752"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="922"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="758"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="937"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1014"/>
            <end position="1026"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1041" mass="110391" checksum="4D8EF6A8F606DDDB" modified="2018-12-05" version="1">PPEQGIKMPEMNLNLKGSTIKGNVDVESVRIAEADKKEFESKHGFPLSQIETLSVNNKDQKVDVRGKSCKTENDMYITEQNINKDVKKYAEVEASVQTIPSKSGTKMSMPDVDINLKGSKQKGHMDVSLPKIKTDIKSHKIDIKGPDVDIKGPDGGLEMPKIKMSSLNLKDVDINLKGPKVEGDLDVKMPKIKGDLKGPEVDIEGPDFDMEGPKGGLKMPKFKMPSFGLKGPKVEGPDVDINLPKADIDVKAPDVDIEGAALDIKGPNAKLKGPKFNMPSISGPKISMPDVDFNLKGPKLKGDMDVSGPKIKGDIKSPQIDIKGATVKTNFPTIPGSKPALPNVDINLKGSKLKGEFKGEGQFPKAELKDIDTGSREGLDVDIGLSGSKSKSPKFKIPKFGLKGLKDVDLNLTGPKLKGDLNVSVPDVDLNLKEANIKGPKFEVEGLKGGLEMPKIKIPAFGIKLPQLENSNDTLTIPNVNADTVTPKVDIGGVDVKGPEASHNVKLPSVSGPTLPDLNINLTGPKTKGNVNVNLPDTNIDIKAPKTEIKGAGKITPPEVDIKGPHVDLGGGKDGFEIPKIKMTSLDFKGPKTGSQGVDINLSKAKIDVDSSKVDFKAPKVKLPTVSGPEWDINLKGSNVEGKEINVNFPKTDIEIKGPKLSMSDVDMNLKAPDVDIKDAKVHTGGPKVGFKMSKVTLPSESEVDVNFAKADFNIKAPEVNIKGPEFDSTEASLDVPDIDVKGKKGKFKLPKVKGKARKQEADIKTPEVDQGIDTPNIHVKGTNTKKPLFGKLHFPDVELDIKSPKLKGDGSLAEGFKSPDANVSSDSAKICLEGPDVNVKGGPEISARTSGQSASGLQYPEGTILFPKIKVPKFGILQPKVEGQDVAGKGKSDAMMGGKLDLEGESGVSLSAKGKSASLDLFKKSKHRSSSASDEGSLGASSPSALSGAECGDISIDVGGTKVKGKKGKLKFGTFGGFGSKSKGSYEVTLGNDNEGEIEGSTDASVPLKKSRLSSSSSSDSGSRGAFRLPKLELSVSPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXU2</accession>
    <name>A0A3B5KXU2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TRIM32</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXU2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003377"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003415.1">
        <property type="protein sequence ID" value="ENSXCOP00000003377.1"/>
        <property type="gene ID" value="ENSXCOG00000002666.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160949"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14961">
        <property type="entry name" value="NHL_TRIM32_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16587">
        <property type="entry name" value="RING-HC_TRIM32_C-VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000034">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25464:SF3">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25464">
        <property type="entry name" value="TRIPARTITE MOTIF-CONTAINING PROTEIN 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_RING_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="7 9" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="20"/>
            <end position="65"/>
        </location>
    </feature>
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        <location>
            <begin position="96"/>
            <end position="139"/>
        </location>
    </feature>
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        <location>
            <begin position="385"/>
            <end position="428"/>
        </location>
    </feature>
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        <location>
            <begin position="441"/>
            <end position="484"/>
        </location>
    </feature>
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        <location>
            <begin position="490"/>
            <end position="525"/>
        </location>
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        <location>
            <begin position="588"/>
            <end position="631"/>
        </location>
    </feature>
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        <location>
            <begin position="639"/>
            <end position="672"/>
        </location>
    </feature>
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        <location>
            <begin position="140"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00504"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="680" mass="74352" checksum="48AE8D7DCCE7B98B" modified="2018-12-05" version="1">IMAAACSSLDPDLMREVLECPICLETYNQDKLRPKLLQCGHTVCRLCLEKLLANTINGVRCPFCSKVSRMSSISQLADNLTVLKIIDCTLTCSAAATALMCKSCGNRLPRQFCYDCTTVLCEVCKADGHLHEGHSVQPIKVAAEQRRKELSGRLAALRDVMGNIQKKKTTLENISKSLRQKYRAVQQEYSSAELHLQEELSKSRRTFTASLGEVEKLNAQVLEEQTYLLNLAEVKVVSRCDYLTVRVRQSDIALLKDDGAGSDDEELDLRSSLPTQFQLQEPTLARAEHSNPIEVGCLTTNTYTVNTDDEKGGLETMLEFKAEDAPSGATGGSVMVPGDLYRDVDMVSAVDETVCGSPSSFKSKSMDAGGESAGASSRPPVCQFVKKMGCKGALPGMFNLPISICVSPQGDILVADRGNCRVQIFNRKGVPREIRRNASIDNFVLSFFGADLPNLIPLSIAVTPQGLIGVTDNYDNSVKVYTMDGHYVACHKNQLIKPWGITAMPSGQFVVSDVEGGKLWCLAVDRNVGVVSYNRLCSAVRPKFVTCDAAGTVYFTQGLALNFEKRQNEPHFEGGFSIGSVGTDGQLGKQLSHFFSETEDFRCITGMCVDANGDLLVTDSGRKEILQFPKEGGFNILIQEGLTCPVGVATTQKGQLLVLDCWDHCVKVFTYVQRRHSSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXV3</accession>
    <name>A0A3B5KXV3_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSXCOG00000003872.1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="78" mass="8122" checksum="6E4372A83FA34A0D" modified="2018-12-05" version="1">IPVMTGAFMDSSPNDDYSGEHSLFNSSASVNAAASAASVHGQPDEQQSMSSDAIWLWIAIIATIGNIVVVGVVYACTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L091</accession>
    <name>A0A3B5L091_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Estrogen-induced osteoclastogenesis regulator 1b</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EEIG family.</text>
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    <dbReference type="Ensembl" id="ENSXCOT00000001247.1">
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        <property type="gene ID" value="ENSXCOG00000001006.1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="2"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="268" mass="29060" checksum="FBC430BE4FE562D6" modified="2018-12-05" version="1">MAFLIKKKKFKFQVHFVLEELAAVPFVNGVLFCKVRLVDGGDFSISSSREEVQQNCVRWGKKFSFVCKMSASPVTGVLDPCICRVSVRKLGFADLNISEFAGSGSTVRCCILEGYDTKNTRQDNSILKVTIGMTLLSGDPCFKTPPSTAKSVLIGVEDPNLQLACKGDSTASLQPPGGMVEGPGAVKARQRKKDTVERQRSCVDDTRIDADDIVEKIVQSQNFSASSSDEDSHLRLFVSKDGTTALSGTQIATRGTPGVFEPVVIETH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L092</accession>
    <name>A0A3B5L092_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SUN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUN" evidence="1">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="181" mass="20587" checksum="B824A7C666C45F70" modified="2018-12-05" version="1">MVMEALKLFSQDRTGLADYALESGGRERTLVYRFVLTVHFSKNNPAFFPPQPDVHPGNCWAFRGSTGFLVIQLSMKILPTAVTMEHVPKSLAPSGTLRSAPRDFSVYVRDFIVFFYFQGLKDEDQDRGKLLGNYTYDEDGEDLQMFIISVSESLANVLSNWGHPEYTCMYRFRVHGLPSDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L0L1</accession>
    <name>A0A3B5L0L1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Prolactin releasing hormone receptor 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L0L1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005863"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005929.1">
        <property type="protein sequence ID" value="ENSXCOP00000005863.1"/>
        <property type="gene ID" value="ENSXCOG00000004550.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164214"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004983">
        <property type="term" value="F:neuropeptide Y receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
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    <sequence length="176" mass="19675" checksum="BE57FB8721970737" modified="2018-12-05" version="1">MFDYKPATFLLQCTYGQEAMEANRSSPTGSTPPSSSAEQPEGRIYEVAVQSNSTNRSSQFADVALLQTFKPLIIPCYVLVVVVGVVGNYLLLYVICRTRKMHNVTNLFIGNLAFSDMLMCVTCVPFTLAYAFNPHGWVFGRSMCYLVFLIQPVTVYVSVFTLTAIAVDRWSPKRRS</sequence>
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            <begin position="32"/>
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</entry>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051868">
        <property type="entry name" value="ZN346_ZMAT4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46144:SF5">
        <property type="entry name" value="ZINC FINGER PROTEIN 346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46144">
        <property type="entry name" value="ZINC FINGER PROTEIN 385B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="U1-type" evidence="12">
        <location>
            <begin position="48"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="85"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="132" mass="15265" checksum="3DDF409CC0B8A8B1" modified="2018-12-05" version="1">MWPALWILQTREYAGREPRQRTNSRAHCLFCFIIANVNKMIRERGDLFSDTQCKVCSAVLISQSQKLTHYQSKKHANKVRRFLSLENENADPVKKPKTSDNVSEKPQLDEKSDANRMWSKLKTSAGLEIITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4G2</accession>
    <name>A0A3B5L4G2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tubulin-specific chaperone C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Tubulin-folding cofactor C</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Tubulin-folding protein; involved in the final step of the tubulin folding pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBCC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L4G2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005275"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005336.1">
        <property type="protein sequence ID" value="ENSXCOP00000005275.1"/>
        <property type="gene ID" value="ENSXCOG00000004127.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162058"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1250:FF:000001">
        <property type="entry name" value="Tubulin-specific chaperone C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.70:FF:000007">
        <property type="entry name" value="tubulin-specific chaperone C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1250">
        <property type="entry name" value="Tubulin Binding Cofactor C, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017901">
        <property type="entry name" value="C-CAP_CF_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006599">
        <property type="entry name" value="CARP_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027684">
        <property type="entry name" value="TBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031925">
        <property type="entry name" value="TBCC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038397">
        <property type="entry name" value="TBCC_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012945">
        <property type="entry name" value="Tubulin-bd_cofactor_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15139">
        <property type="entry name" value="TUBULIN FOLDING COFACTOR C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15139:SF0">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07986">
        <property type="entry name" value="TBCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16752">
        <property type="entry name" value="TBCC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00673">
        <property type="entry name" value="CARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51329">
        <property type="entry name" value="C_CAP_COFACTOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-CAP/cofactor C-like" evidence="12">
        <location>
            <begin position="161"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="148"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="346" mass="38701" checksum="20AE22C13FD79964" modified="2018-12-05" version="1">LRIVSEYKKKSEENGFYGRNAAAKLHERIEKRQQVLAEDVERRKEARQSQSVAEEKSEYFVSSFNAERAGIQELLSDCSGADPSTVTQKLEEATAKMTQLQKFLNDSMIFLSQYQIRVAQGALQKLQASLNETREEALPKKKFTFRSRTKAAEKVSDSPPPPSSETVSSGVTKVDEATISEQTGFSNISNESLTKTSEEIQSRDVLLTHLTNCKVRLYGSPSTLHLKHITACEVLCGPVATSVFVDHCSNSVLGFACQQLRTHNTTDTQVYLHVTSRAIIEDCRGVSFAPFCWSYPTLDQDFTASGLDQDRNNWSQVDDFNWLAAGTPSPNWTVIPEADRKTTWDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4N8</accession>
    <name>A0A3B5L4N8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Spondin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027864522.1">
        <property type="nucleotide sequence ID" value="XM_028008721.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027864523.1">
        <property type="nucleotide sequence ID" value="XM_028008722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L4N8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005360"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005421.1">
        <property type="protein sequence ID" value="ENSXCOP00000005360.1"/>
        <property type="gene ID" value="ENSXCOG00000004192.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114139071"/>
    <dbReference type="KEGG" id="xco:114139071"/>
    <dbReference type="CTD" id="30201"/>
    <dbReference type="GeneTree" id="ENSGT00940000165836"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000037">
        <property type="entry name" value="Spondin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2130">
        <property type="entry name" value="F-spondin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051418">
        <property type="entry name" value="Spondin/Thrombospondin_T1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009465">
        <property type="entry name" value="Spondin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038678">
        <property type="entry name" value="Spondin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044004">
        <property type="entry name" value="TSP1_spondin_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038123">
        <property type="entry name" value="NF038123_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11311:SF33">
        <property type="entry name" value="MINDIN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11311">
        <property type="entry name" value="SPONDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06468">
        <property type="entry name" value="Spond_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19028">
        <property type="entry name" value="TSP1_spondin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51020">
        <property type="entry name" value="SPONDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017464746" description="Spondin domain-containing protein" evidence="9">
        <location>
            <begin position="28"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Spondin" evidence="10">
        <location>
            <begin position="34"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="334" mass="37266" checksum="0822A4F258E08C28" modified="2018-12-05" version="1">MMSSEHPACGWLHKLLVLLVKLHFAFAEPLQPLNGTGCTAKGPASYILVFTGHWSPQAFPKQYPLFRPPAQWSKLIAASHNHHFRFWEEGSSASPGVQSFAEVGVTVELMKAAKEARKKRTIGVMYRTAGIPNGIGHSSTEMLMVPRNPLLSLMVKMIPSPDWFVGVDSLNLCEGNRWKQEVTVDLQPYDAGTDSGFTFSSPNFPTSPPENITKITSQMPNHPANSFYYPRLKELPPIASIKITRQRRTPDRQTSISNHILPNSINPQRFSATPLDCEVSLWSSWGLCLGPCSRGGVRHRTRYILLRPANAGSPCPELEEQDECVPHNCIKKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L502</accession>
    <name>A0A3B5L502_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Si:ch73-389b16.1</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L502"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005480"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005542.1">
        <property type="protein sequence ID" value="ENSXCOP00000005480.1"/>
        <property type="gene ID" value="ENSXCOG00000004277.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153190"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18875:SF8">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18875">
        <property type="entry name" value="SARCOMA ANTIGEN NY-SAR-24/CYTOSKELETAL PROTEIN SOJO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="40"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="131" mass="15376" checksum="C4620EC24B0AFB9C" modified="2018-12-05" version="1">MSKFPGKLTQREQGLLRIRRDSDTKAAELAKMEKMLQQTKNMMEKKTDSGPESKSYQENMEDLEEKVRSTRRYRRNSLHHTQMLESQMKTVKGELVGTLDHLQELRNVLRRSQQKAEERKVAMEKLAAGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5B8</accession>
    <name>A0A3B5L5B8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="33">adenylate cyclase</fullName>
            <ecNumber evidence="33">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="38 39">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="38" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="38" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="32">Catalyzes the formation of cAMP in response to calcium entry leadings to cAMP signaling activation that affect processes suche as synaptic plasticity and insulin secretion. Plays a role in many brain functions, such as learning, memory, drug addiction, and anxiety modulation through regulation of synaptic plasticity by modulating long-term memory and long-term potentiation (LTP) through CREB transcription factor activity modulation. Plays a central role in insulin secretion by controlling glucose homeostasis through glucagon-like peptide 1 and glucose signaling pathway and maintains insulin secretion through calcium-dependent PKA activation leading to vesicle pool replenishment. Also, allows PTGER3 to induce potentiation of PTGER4-mediated PLA2 secretion by switching from a negative to a positive regulation, during the IL1B induced-dedifferentiation of smooth muscle cells.</text>
    </comment>
    <comment type="function">
        <text evidence="33">Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 33">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="35">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="35">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="35">Binds 2 magnesium ions per subunit. Is also active with manganese (in vitro).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Basolateral cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasmic vesicle</location>
            <location evidence="8">Clathrin-coated vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane raft</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <location evidence="7">Caveola</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="31">Membrane</location>
            <location evidence="31">Coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="29">Postsynaptic density</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="30">Presynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="33">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="33"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5B8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006007"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006074.1">
        <property type="protein sequence ID" value="ENSXCOP00000006007.1"/>
        <property type="gene ID" value="ENSXCOG00000004622.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167206"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030665">
        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050796">
        <property type="term" value="P:regulation of insulin secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000001">
        <property type="entry name" value="Adenylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000011">
        <property type="entry name" value="Adenylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032628">
        <property type="entry name" value="AC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030672">
        <property type="entry name" value="Adcy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009398">
        <property type="entry name" value="Adcy_conserved_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF15">
        <property type="entry name" value="ADENYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16214">
        <property type="entry name" value="AC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06327">
        <property type="entry name" value="Adcy_cons_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039050">
        <property type="entry name" value="Ade_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17 33 34" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="20 33" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="27" id="KW-0966">Cell projection</keyword>
    <keyword evidence="23" id="KW-0168">Coated pit</keyword>
    <keyword evidence="28" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="24" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="26 33" id="KW-0456">Lyase</keyword>
    <keyword evidence="18 33 35" id="KW-0460">Magnesium</keyword>
    <keyword evidence="25 35" id="KW-0464">Manganese</keyword>
    <keyword evidence="22 33 36" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 33 35" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0488">Methylation</keyword>
    <keyword evidence="16 33 34" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="39" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0677">Repeat</keyword>
    <keyword evidence="21" id="KW-0770">Synapse</keyword>
    <keyword evidence="13 36" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19 36" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="36">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="36">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="36">
        <location>
            <begin position="267"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="36">
        <location>
            <begin position="314"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="36">
        <location>
            <begin position="690"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="36">
        <location>
            <begin position="717"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="36">
        <location>
            <begin position="800"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="36">
        <location>
            <begin position="828"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="36">
        <location>
            <begin position="861"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="37">
        <location>
            <begin position="403"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="37">
        <location>
            <begin position="948"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="binding site" evidence="34">
        <location>
            <begin position="408"/>
            <end position="413"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="35">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="35">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="35">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="34">
        <location>
            <begin position="450"/>
            <end position="452"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="35">
        <location>
            <position position="452"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="35">
        <location>
            <position position="452"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="34">
        <location>
            <position position="496"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="34">
        <location>
            <position position="1000"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="34">
        <location>
            <begin position="1070"/>
            <end position="1072"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="34">
        <location>
            <begin position="1077"/>
            <end position="1081"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="34">
        <location>
            <position position="1117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
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            <dbReference type="ARBA" id="ARBA00004187"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004345"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004640"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00034105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00034111"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00058052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="PIRNR" id="PIRNR039050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="PIRSR" id="PIRSR039050-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="PIRSR" id="PIRSR039050-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="37">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="39">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1199" mass="137156" checksum="428191264CC4FA54" modified="2018-12-05" version="1">MPAEVTMVTFADTRQVSPMELHEPPCLSATLSPGLRRKKMLWQNAVKHIITQQELSSQVIHGSVYTSVFLCSSCISRKIFVTDTYIDEINKQIRSKASRGVTKRRSSTFRVHSRTSTSTHGSIRVPTQHYTSDADFFVHWCNTVHSVYIPMLGHTFKSHDLEKLYQQYSSHQRRNSLAITNVIDAVTKLHMLVLYLALAPDAVTDRLRGYLTGIFMVLAIALCVLVLTCKDSMSPQWLHYAGFASWLSQTTQILGGLAYGLEKDPSWYILFTLFATYTLLPLPLLWAMCAGFLTPILHILMEIWFHYNDVFAKGLLYMGMNTAGLFIHYLTDHAQRQVFLETRRCIEGRLKLEQENQRQERLVLSILPRFVALEMIADMSSLENELNPHEFYKIYIHQYKDVSILFADIKGFTQLSINLSPQEMVLTLNELFGRFDRLAEEHHCLRIKILGDCYYCVSGVPEPQLAHARYCVEMGLAMISTIRLVQKQLNIDLDMRIGIHSGSILCGVLGLQKWQFDVWSWDVSIANKLEAGGIPGRIHISRATLDCLGTTYKTEDGHGRDRNEFLRKHNIDTFFICPQEGRNQDGHPEPIKVQKRIRTWNPEIPFGHAIDMNCVSVGTQNRFKEFTFMCLLFSFLFASCTSREINKRIKHAIEVRSSERMRKEHITALTLVFKDTHIENKFSQIRDEMFNSNMVCSFIVLLFLMAAQAVIPTPRLFSAVLQFFLFLLAYWLLLLVALAEEFKWTPVALQKFCCWIHENNSARNLLTLTAIITNFSLASTDLVRVGMDTRDEKMQHTYSTYTAIFVLSGVVAMVTCGVFLRLNSLLKLAVLLMVVMVYSYLIHLAFVTLTRSDAQHRSVCLRRKWISILLMAMFIVSVFYNGRQWEATARLDFLWRLQAQQEVEDMRELREHNECLLHNILPLHVAQHFLERSRHDEDLYSQSYDEVGVMFASVAGFNEYFEQKEVKHEGVDCLRLLNEIIAGFDELLEESYFHYVEKIKTIGSCYMAASGLAPNKQWNHLSELVLFALAMQEILREINNHTAQSFELRVGIAHGPVIAGVIGASKPQYDIWGSTVNLASRMDSTGVSGRIQVPEATRKILAEWGFVLKLRGEIFIKGVSECQGKVRTYFISTMRSKRGRVGGEKYSGGRAGGRMTLAGVVHGLVQARHKEKMREANGNFGLTPLNHMVQKWCFPKFTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5C6</accession>
    <name>A0A3B5L5C6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Hyaluronidase</fullName>
            <ecNumber evidence="10">3.2.1.35</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Random hydrolysis of (1-&gt;4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.</text>
            <dbReference type="EC" id="3.2.1.35"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 10">Belongs to the glycosyl hydrolase 56 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.35" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5C6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005605"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005671.1">
        <property type="protein sequence ID" value="ENSXCOP00000005605.1"/>
        <property type="gene ID" value="ENSXCOG00000004356.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230364"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004415">
        <property type="term" value="F:hyalurononglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030214">
        <property type="term" value="P:hyaluronan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000065">
        <property type="entry name" value="Hyaluronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018155">
        <property type="entry name" value="Hyaluronidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11769">
        <property type="entry name" value="HYALURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11769:SF20">
        <property type="entry name" value="HYALURONIDASE PH-20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01630">
        <property type="entry name" value="Glyco_hydro_56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038193">
        <property type="entry name" value="Hyaluronidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00846">
        <property type="entry name" value="GLHYDRLASE56"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="8">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="46"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="209"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="364"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="369"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR038193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR038193-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR038193-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038193-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU610713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="489" mass="55146" checksum="1F891E6CCDE8EF78" modified="2018-12-05" version="1">HTAHFDRHLFLSSGFVPGFLALPPTEPPLVRDHPFVVIWNAPTDQCKQLEIPLDTAAFQAVTTPSAVPGQFLTIFYEDRLGLYPKVDIIKHKIYKGGIPQNGNLTEHLAKAKRTIDHYISQDSSPGLAVIDWESWRPLWDQNWGSKHIYQKLSITHALHLAPFLTTKKISQTAKSQFELAGRRFMEKTISIGIGKRPSRRWGFYLLPDCFNYGWNKPGYTGRCSTKAQKQNNKLLWLWERSTALFPSVYLHMTLRNSPLAALYVRNRVQEALRVAALPKHLYTAPVYETGIWYENVFVTCVFLSFQTDLVNTLGESAALGASGVVIWGGTRDYNSKASCQALSEYLSSTLSPYVANVTAAAMLCSRLLCKGNGRCVRKNYNTAHYLHLNPSSFRILKASGKYVAVGLPSASDLSNWVENFTCQCYAGWSCFPKLRRNHIISNVCSQNKDMRQNIKFPVSCNGIFFFLHTVAENKLCVVQHICNPVLAEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5D8</accession>
    <name>A0A3B5L5D8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5D8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002974"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003011.1">
        <property type="protein sequence ID" value="ENSXCOP00000002974.1"/>
        <property type="gene ID" value="ENSXCOG00000002363.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00890000139436"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="2251" mass="251949" checksum="3FAEFCA05FD0B649" modified="2018-12-05" version="1">FFEPEKMISTIKEQDEDPDDFCTHLDEVTAELSSCFDDLNKPPPLHTGADWKVVLHLPEIEKWLRATSDRVAQLTHSVGQDTDNRHVDVHLVQLKDICEDISDHVEQIHALLETEFSLKLLSYSVNIIVDIRTVQLLWHQLRVSVLVLKERLLQGLQDPNGNYTRQTDILQAFSQDQHQTRLDALTEVDDCGQLTIRCSQDYFSLDCGITAFELSDYSPSDHPEDRGTESTVEDTSQNIIHTTNSNLELHKSLPQHSLPSDIDNHSHSTPETPTNQSDMPKYSESSKRPLQAVSHSTESSPTQPSLPKRAALFSNKGTKSRGSFRKARQQSELQHKAELSQSTPSLVNPPDRSKFWLELDSVDLRTLPQSNESLQKTIEDNLKNHHVSSTHRKPEGSSGANDSLPVCRLMSLDEISTQTERRQKRTYAVSMGDSDSSLPSPISEQLLSSDLEKGSQVPSERIQSREHWYGSDEFLALPAQLRETEMLAVKLESLIQSSTLQKQRGLQDVDDWELSELNVDWDHPHRQTSVSCFSSSSSDVAPSLEDSIESEPLSDLQSGEEQSRRSVDGSPQLTAPLVDGHGCASLMKKLLDDIQDQNEGIWVKMEVIPYTQFAPMFCINLSFCWFCFHLSLKDILLMVGSQWDQLQWQIRRQHGWMLRALRCIQTRLLYTRVSALTSRYRVMLHNILPVVWMKPLSLANKSQPIISKVLITANKLMNLFLSIISLQEFEAEYQELWEWLMDMDAMVTDSHQLMMSEEQRHHLFKVRWTLLMMKSRKTSLLRQAAKHSVPCSSEQKLSSNANTLLVSGQDVAAESPRSALSPMTNSLLEQLEARIKELKAWLRDTELLIFNSCLRQETCAFEQLSSFKSLCSEIGSRRRGVASVLKLCQKLLCNLLNSALQLLSINLERRWEAIVMQALQWQNRLKRDPVSVASGTVPPEDSWEWDETDILLSNNILATDNQPVEIQTDIQATQLKEFSNLKDGSLIDVNSHSPPNNNVYQVYSLHSIELCKQPVFPLPSTLMHINKPVKNKEHFLLKSISKDSSLSSIESLPDLLGGLMSSSQQRERNSEFCNRESEGGRRSGQSENSLRSDCESGIVSDNGDSETSSNSGVQGKKEDQGIERKEKRDQRKGEVTQEHYSLALEKNCIAPYKRSKMQISMNLEEWRRNELKCEIKRNKREEKKRCKQDGGEAVEFLINGHGILTPANSNSDIDVKGLDDSKMFISQRDNTECNPDHYNSSETPPVLNSRTSSPVLSQSSSLESLLALGIDLFPSKERLHHSVSLESCLNPCHSPGREAVGSLTSPDEMNPCQEKDQDRINLRASIDTETEASSGELSQRTLDLLKRLENIQNPLVGKMTRSVSDMSIQSSIPQKFHFPVSPSLGEPSLINENLSKMSLTELSSTEDSSLASEDFIVLRNQHPLFLDPNIATSSSPRRKHCHNGNRGGKAVDESDAASLSMVVNVSCTSACTDEDEDDSDLLSSSTLTLTEEELDEDNDEDDMENSYELGLEYMKRELQSWIRPPRTSVSSSFKSEAGLLDELQCGSAPPSTFTYSRAQLMDSSNKAGRNEREDETRLNVTRSYISQLVDDVENGNIDHSCLQGKDEDNELLQEESGLFTQKRDTLRDFYVNTKTEDQVEDVGNLRVEMDSVSTSHICSSPSSELHPFSSKRTSCLVGELRGELPCHSASFPSPPLLSPVNCRSHNALDNNFSIAGGRKAITIQEKFKFSSLVTEETRREVRAKDSSLPSKKGHKSHASCCSPFVLSASPLSVDESKKENVHDFVMEIIDMASVALKSKENQSDGATQREISGSVPDQSSTSLAHIRDKVLEHSHRPLHLRKGDFYSYLSLSSHDSDCGEVSQCAEDKSLTPVPYTIPSYCTSTPTPNSPSPELFPSSKVAMSSFQSSQLSRRSSPNLTAKDYIGSLDLKCFDGEPGSISDGSRVEQQSSSPSLSVPTSPDFRNEETLFEACTEEVYLGPPLCYSMVIKKKPQPSYHKLRLDTSLAGGCELIENGPCLSQEGKSSKLDNRRKDHFFSTQGSGSRNETQNFYLPEPCYSLFSSSSLLRSNFSSSGDNLFSPLPVSTEAKEEKLGEDLYLSSDGVVGKVATAVSVSTPQEERSRNEGPSYLNPWVRVALIDSFATECLADTKTLESNMGTVMTKISVCSSATNPSKEPATAATRINPKINCSAMRRPDREEGKRSKEEDTRWAEEEEKRRKGWSILQQEAKSALVKQVSVPCICTISRHYTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L625</accession>
    <name>A0A3B5L625_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peripherin-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Retinal degeneration slow protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRPH2/ROM1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L625"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005276"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005337.1">
        <property type="protein sequence ID" value="ENSXCOP00000005276.1"/>
        <property type="gene ID" value="ENSXCOG00000004130.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157303"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03162">
        <property type="entry name" value="peripherin_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000002">
        <property type="entry name" value="Retinal outer segment membrane protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000830">
        <property type="entry name" value="Peripherin/rom-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018498">
        <property type="entry name" value="Peripherin/rom-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042026">
        <property type="entry name" value="Peripherin_LEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF202">
        <property type="entry name" value="PERIPHERIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00218">
        <property type="entry name" value="PERIPHERNRDS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00930">
        <property type="entry name" value="RDS_ROM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="355" mass="40700" checksum="B2EF527A4DF44140" modified="2018-12-05" version="1">MALLVVKFDLKKRVKLAQTVWFMYWFAVLAGILVLSLGLFFKIELRKRSELMDNNESHFLPNVLIAMGLIACGINAFGGKVCYDSLDPTKFAKWKPMLKTFLVFCVGFNALLIVTSLLCFIMRIPLQFTLAEGLKNGMKYYKDTDTPGRCYMKRTLDLMQMEFRCCGNDNYRDWFEIQWVSNRYLDFSAKEVKDRIGSNVDGQYLMDGVPFSCCNPSSPRPCIQYQMTNNSAHYSYDHYTEELNVWRRGCRDALLSYYGGIMNTIGALVLLVTMLEVTISCLLTVFIQSIHIVLQNCFLSEISDTLKRTANLSLSLFCFLSVSLSSTHTYTHTHTVMGTNRQLLVFPSDLMSRLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L661</accession>
    <name>A0A3B5L661_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Aspartyl/asparaginy/proline hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartyl/asparaginyl beta-hydroxylase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L661"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003259"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003298.1">
        <property type="protein sequence ID" value="ENSXCOP00000003259.1"/>
        <property type="gene ID" value="ENSXCOG00000002566.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156304"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062101">
        <property type="term" value="F:peptidyl-aspartic acid 3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007803">
        <property type="entry name" value="Asp/Arg/Pro-Hydrxlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039038">
        <property type="entry name" value="ASPH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12366">
        <property type="entry name" value="ASPARTYL/ASPARAGINYL BETA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12366:SF33">
        <property type="entry name" value="ASPARTYL_ASPARAGINYL BETA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05118">
        <property type="entry name" value="Asp_Arg_Hydrox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aspartyl/asparaginy/proline hydroxylase" evidence="2">
        <location>
            <begin position="9"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="101" mass="11703" checksum="0BCBC8C0AAE878BC" modified="2018-12-05" version="1">SAISTYGEAFSVMQPGTHVWPHTGPTNCRLRMHLGLVIPASGCRIRCTDQTREWEEGKVLIFDDSFEHEVWQEADSYRLIFIVDVWHPELSQYQRQTLSPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6E4</accession>
    <name>A0A3B5L6E4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Bromodomain and PHD finger containing, 3b</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6E4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003344"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003382.1">
        <property type="protein sequence ID" value="ENSXCOP00000003344.1"/>
        <property type="gene ID" value="ENSXCOG00000002639.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155056"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05512">
        <property type="entry name" value="Bromo_brd1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15572">
        <property type="entry name" value="PHD_BRPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000008">
        <property type="entry name" value="Bromodomain containing 1, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000007">
        <property type="entry name" value="Bromodomain containing 1, isoform CRA_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF19">
        <property type="entry name" value="BROMODOMAIN AND PHD FINGER-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="193"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="14">
        <location>
            <begin position="247"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="12">
        <location>
            <begin position="559"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="367"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="684"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="695"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="754" mass="84998" checksum="D5B024C32D86B50D" modified="2018-12-05" version="1">MRKPRRTCQVGGGGRRSPSPYSLKASPSRETLSYSQSQKVVEVELDGRLHRISVLEPLEVITDDETVAQDISECNSNKENSERSVSTSNSTHITLPEPKFRVVESFTPVEAPPLPAAYYRYIECSPEDQERKAEYDMDEEDSAWLEMVNATRLSEGLSAISPDNFELLVDRLEEEAYREARIRAPPQSAIDDDAFCCVCLDDECLNSNVILFCDSCNLAVHQECYGVPYVPEGQWLCRCCLQSPHKPVDCVLCPNRGGAFKQTSEGRWAHVVCAIWIPEVCFANTVFLEPVEGVGNIPPARWKLTCYLCKQKGRGACIQCHKANCYTAFHVTCAQRAGLFMKIDPVRETGINGTTFSVKKTAFCETHSPLGQESESDEESEGRGRIAYTDGPTLPKKGRKCEDEPADKKKGKKNTECTTQTTGSPQLVCLFLICYRLNIISKGILMQRKNQFMHRLHSYWLLKRQSRNGVALVRQLHSNIQSQKSTEQPEVDEKISAAREALRYWQKLRHDLEKARLLVELIRKREKLKREQVKVHQSTLEMQLTPMLMLLRATLDHLQEKDTAHIFAQPVNLKEVPDYLEFITYPMDFSTMRSKLESHAYRSVADLEADFNLMVSNCLLYNTKDTVFYKAALRLRDLGGAVLRHAQRQATNAGLDLDTGMHLPESPQKRDFYSCTWEDGKNPVFNGKGLQDENTSSNPTGPSSTTSSPSCTPASRTPQKSPGPPTLHEVWTPGREASSDTELERTVNYSLESG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7Z3</accession>
    <name>A0A3B5L7Z3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L7Z3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003909"/>
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        <property type="protein sequence ID" value="ENSXCOP00000003909.1"/>
        <property type="gene ID" value="ENSXCOG00000003075.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="SZT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14918">
        <property type="entry name" value="KICSTOR COMPLEX PROTEIN SZT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14918:SF3">
        <property type="entry name" value="KICSTOR COMPLEX PROTEIN SZT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="180" mass="20528" checksum="4B1F02083DCE4CA3" modified="2018-12-05" version="1">MQHHVFCASHYKVEEADQIFLLMKEDYRISRNVRLAWFLRNLNQIIWPASTSELQNFENELDLAAVHPKGWQSDSIPTTAPCVLMPSTRATFLARRYRFIIELDLSPSTGIVDDSTGEMIFDEVFHALSKCLAGLAQPFKVPGTDQLFKPKIFITILVYSSIIGLSSHQVICYPVFHPLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8V7</accession>
    <name>A0A3B5L8V7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HBS1-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">GTPase component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. The Pelota-HBS1L complex recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the Pelota-HBS1L complex, also named Dom34-Hbs1 complex, composed of PELO and HBS1L. Interacts with the SKI complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8V7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006885"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006973.1">
        <property type="protein sequence ID" value="ENSXCOP00000006885.1"/>
        <property type="gene ID" value="ENSXCOG00000005323.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156274"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16267">
        <property type="entry name" value="HBS1-like_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04093">
        <property type="entry name" value="HBS1_C_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000039">
        <property type="entry name" value="HBS1-like translational GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000035">
        <property type="entry name" value="HBS1-like translational GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000020">
        <property type="entry name" value="Translation elongation factor EF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000204">
        <property type="entry name" value="Translation elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015033">
        <property type="entry name" value="HBS1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037189">
        <property type="entry name" value="HBS1-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08938">
        <property type="entry name" value="HBS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109732">
        <property type="entry name" value="HBS1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="Tr-type G" evidence="15">
        <location>
            <begin position="254"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="124"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="169"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="679" mass="74382" checksum="F0240A9C2163990D" modified="2018-12-05" version="1">MSRHRNVRGYNYDEDFEDDDMYGQSVEDDYCISPATANQFIYSRRELQAPKEEPLEEEEYEDEDVPMSPCVDFNLDPLNQAKLFSCLDHMRAVLGDAVPDSVLTQAAMKHGFDPQRALDAVLSEDSKTAPVTRSAAEDMPSVAKSRDAAPDKLDSEVVPKVARMTVSGKKQTMGFDVPSSGENGVVASPARRGDSPENTKATPVPEAAPKRPETPSKASNGDEISASATPGRSSGKTRHAINIKAELERRQGGKPLLNLVVIGHVDAGKSTLMGHLLYLLGNVNKRTMHKYEQESKKAGKASFAYAWVLDETGEERDRGVTMDVGMTKFETNSKVVTLMDAPGHKDFIPNMITGAAQADVAVLVVDASRGEFEAGFEAGGQTREHALLVRSLGVTQLAVAVNKMDQVNWQQERFQEISSKLGHFLKQAGFKESDVFFVPTSGLSGENLTARSTVSELTSWYSGHSLLEQIDAFKAPQRSIDKPFRLCVSDVFKDQGSGFCVTGKIEAGFIQTGDKILAMPPNETCTVKGITLHDEALDWAAAGDHVSLTVTGMDIIKINVGCVFCDPKEPIRVCTRFRARILLFNIEVPITQGFPVLLHYQTVSEPATIKKLISVLHKSSGEVLKKKPKCLSKGMNAVVEIQTQRPVSLELYKDYKELGRFMLRYVGSTIAAGVVTEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9M4</accession>
    <name>A0A3B5L9M4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Helt bHLH transcription factor</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027873380.1">
        <property type="nucleotide sequence ID" value="XM_028017579.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027873381.1">
        <property type="nucleotide sequence ID" value="XM_028017580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9M4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007282.1">
        <property type="protein sequence ID" value="ENSXCOP00000007190.1"/>
        <property type="gene ID" value="ENSXCOG00000005555.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114144585"/>
    <dbReference type="CTD" id="391723"/>
    <dbReference type="GeneTree" id="ENSGT00940000160388"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="8">
        <location>
            <begin position="49"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="107"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="196" mass="21934" checksum="ECDED88F5580495A" modified="2018-12-05" version="1">MALAKQNSGKLEKAEILEMTVQYLRALHSADFPRGREKGELLAEFANYFHYGYHECMKNLVHYLTTEDRAETKDIKYARILAFLQSKSRAVTDPVFGSVGSMSDPPDYLGHLHSSPEHQSHSPSDSVYQQSPTGHFSWHGSGRSQGISYPAVPLSAHTQQHGGYLSPVQGLDHHYFNFIGHTHANTFSLHSAQHAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBJ6</accession>
    <name>A0A3B5LBJ6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Discs large MAGUK scaffold protein 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000007885"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007983.1">
        <property type="protein sequence ID" value="ENSXCOP00000007885.1"/>
        <property type="gene ID" value="ENSXCOG00000006053.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155303"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035331">
        <property type="term" value="P:negative regulation of hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01671">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06764">
        <property type="entry name" value="PDZ1_DLG5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06767">
        <property type="entry name" value="PDZ3_DLG5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06766">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11860">
        <property type="entry name" value="SH3_DLG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000130">
        <property type="entry name" value="Discs large 5, isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000152">
        <property type="entry name" value="disks large homolog 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
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    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006907">
        <property type="entry name" value="DLG5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035537">
        <property type="entry name" value="DLG5_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053004">
        <property type="entry name" value="MAGUK_Signaling_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46360">
        <property type="entry name" value="DISKS LARGE HOMOLOG 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46360:SF1">
        <property type="entry name" value="DISKS LARGE HOMOLOG 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16610">
        <property type="entry name" value="dbPDZ_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04822">
        <property type="entry name" value="Takusan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="CARD" evidence="6">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="688"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="772"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="1379"/>
            <end position="1458"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="1533"/>
            <end position="1613"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="4">
        <location>
            <begin position="1804"/>
            <end position="1934"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="980"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1048"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1262"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1463"/>
            <end position="1519"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="184"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="430"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="980"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1058"/>
            <end position="1069"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1089"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1124"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1268"/>
            <end position="1283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1310"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1340"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1464"/>
            <end position="1493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1502"/>
            <end position="1516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1948" mass="219977" checksum="53D6C433B6CBDBCA" modified="2018-12-05" version="1">MELQHKELLEKCYQNLVESITDADRVVDVLSRRGALSQSELMELGHHCVSGSEKVDLLLKILMSKDRDCFAEFCTALEATHPHLHSLLHGSNFNVFVRPTGSTYSVLSTMPSDSESSSSLSSLASSPLPAHVDVHQTSEKMETVLFQLRQVTRERDELLKRVALPTAGNSIDECRLNSRSGHDYERLKLQCMKAMADLQSLQNQHSTTLKRCEEAVKKADFYRRLASDHNQLKEELEAVRQRNIQLVREHNHVKQTCEELRRLHEEDQREVADMRLLHQQVMREGSSDVLNKLYETTVDKLEAMKSEYEALRKRYNENTASHNADLSRLDQFEEENHRLQKTLDILSKQRDAAIHYQQQFSASVQRFDNTQHELSKATVQNLELQRELERLQSETTRLKTQQLKAVKDCEKYKEERDSVINEYRLIMSERDQVIKEVDRLQTGLEVAEAKLKNNSSERRVANEELEALQQELASALVDRDRAICEKNELLEKYCHEVKDKTEAQKELNQACKDIEIVREERDVARKERTEAIIQRDQLLREYYQARQKQDSATLDMERANKEIEILRKQYEAISQELKEALQEAEVAKCRRDWAFQERDKIVAERESIRTLCDNLRRERDRAVSDLADALRNLDDTRKQKNNAMRELKEQKEKLEDQLETEARYCQLMAHSSHDSAIDTDSIEWETEVVELEKHRDMDLKALGFDIAECVNDPYLPGDSGIFVSKVDKGSVAEGRLRVNDWLLKINDVDLTNKDRKQVIKAVLSWEGVINMVVRRRKSLGGRVITPIQINLAGHKDCGIGLESGVFVATLAAGSPATRDCNLAVGDRLLAINGIELHDRSLSECESLLRTCDNSLSISLMKFLPQSSSGQNLFEGMRDSEKTLRLQSGELHPRGCKNSKHNCSTQTDICNCKSSIRQEEVCKDTCDSLENGGNSSTHSHHKSFSYCSQHSCSLSMSHSLSEPHPAFSYQRQELSPRSLTFTPLVSNCSPPQSPVDRGQSPPVKAGGGTWPKVIVGASLTECTQLSIFKKPKQRKSIFEVNAFRRPETASKLDYMSKHSPQSSASETSQTPPTPPTRSDSFRFKHRQQNSSASDSTITTASPQDEGFVGSQEYYTNIPAGKNKKNFKEGDESRRRTEVQEKRRYRPKSAPALRRNVTPLHIPVPMQTQVSSDELSPEPVDLLRFSPLRTNRYSVPFAPPSYLTAHLPQRRAAPCPAMTAVMRNPVYTAWSHEIQNCPPVPNSGLDQHTQSPQQQAPLSLDLGHKRNGRLSETSHSQPPHSTKSLPSGARLSSPGTLQFRAERIKIPPARYSRSTGSDKASLSHSECSSPTPPISPVNLDTSSFSSSQSQSSVSSHPRISVSPAPVGDRRKDGPFLGEPRNVTVQKGVEPLGISIVSGENGGVFVSKVTLGSIAHQARLEYGDQLLEFNGINLRNANEQQARLVIGQQCDMVSILAQYNPHMFQLGNHSRSGSQMESSNNHLTTSPDNHSAVDTLSEQDEGTMTPPSRQTTPATSPHNSFRLPGSLLLRAAEPRLVRLKRIDTELGVQICGGNVCGIFVEMLDDESPAKTPDGLLPGDLILEYNGVSMKNKTKEDAYLEMLKPADQVMFKVQNYGDSLAVIKESQGDGFFIRALYERVAEQELSFKKDDILYVEDTLPNGNFGFWMAWQLDENAQRLERGQIPSKYMMDQEFYKRHGLADMKDENGTSKSMSAAARRSFFRRRLKHKRSGSKDGKDLMALDSISTDSLPIPEDGMSLMYQRVQKVDCSSPRPVLILGPLVEACKDLLVTEAPANFCRCLPEIMKASQQAIERGVKDCVFIDYKRRSGHFDVTTVASIKEIIEKDCHCLLDIAPHAIERLHSVHIYPIVIFIRYKNAKQIKEQKDPLYLRDKLSQKHSKEQFEAAQKIEQDYSRFFTGVVQGGSVSSICTQISTIVEHEQNKVLWIPDEAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBV7</accession>
    <name>A0A3B5LBV7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Basic, immunoglobulin-like variable motif containing</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBV7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008005"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008104.1">
        <property type="protein sequence ID" value="ENSXCOP00000008005.1"/>
        <property type="gene ID" value="ENSXCOG00000006148.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048601"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171:SF13">
        <property type="entry name" value="BASIC IMMUNOGLOBULIN-LIKE VARIABLE MOTIF-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-G CELLS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="96"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="438"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="463"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="502" mass="56336" checksum="46233C94369168EE" modified="2018-12-05" version="1">MPNTSEGQGANLPRPAEQSELRRPADGEEEEDHGLISSLARDAERLRRASSAELHLQWTCPVTHSREKFYTVCSDYALLNQAASVYCCPPKVARNKQEDGAPLGKPKASSDFSGGHTGGGPVGSDGDCDVDEVSSGHAKPLLAWEIDTADFNTVFTRKIRTNGKKCSSKKMRASDRPSRNLQDVPVLASLEEIKQRKVLDLRRYCISRPQYKTSCGISSLVSCWNFLYSTLGAGLSPISQEEALHILGFQPPFEDIKFGPFTGNATLMWFRQLNDNFRVRGCSYILYKPHGKHKTAGETEGALMKLTQGLKDESMAYIYHCQNHYFCPVGFEATPLKAAKAYGPLPTNEMEHWILIGEPSRKHPAIHCKWMDIVTDLNTQNPDYLDIRHTERGLQRRKTKKVGGNLHCIMAFQRVNWQKLGPWALNLENLRHDFHQPASDRAHGSVADDSEERTPSKRLAQLGRSQSMGSQKDTCSWKRLSNTTEYRQRGSPESDLEEDITD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LD98</accession>
    <name>A0A3B5LD98_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Plasma retinol-binding protein II</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">RBP delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 13">Belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LD98"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008515"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008621.1">
        <property type="protein sequence ID" value="ENSXCOP00000008515.1"/>
        <property type="gene ID" value="ENSXCOG00000006521.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047107"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060417">
        <property type="term" value="C:yolk"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019841">
        <property type="term" value="F:retinol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034632">
        <property type="term" value="F:retinol transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001889">
        <property type="term" value="P:liver development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032526">
        <property type="term" value="P:response to retinoic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000004">
        <property type="entry name" value="Retinol-binding protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022271">
        <property type="entry name" value="Lipocalin_ApoD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022272">
        <property type="entry name" value="Lipocalin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002449">
        <property type="entry name" value="Retinol-bd/Purpurin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11873">
        <property type="entry name" value="RETINOL-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11873:SF2">
        <property type="entry name" value="RETINOL-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036893">
        <property type="entry name" value="Lipocalin_ApoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500204">
        <property type="entry name" value="RBP_purpurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01174">
        <property type="entry name" value="RETINOLBNDNG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00213">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0683">Retinol-binding</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017106961" description="Plasma retinol-binding protein II" evidence="10">
        <location>
            <begin position="17"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="14">
        <location>
            <begin position="36"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="19"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="85"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR036893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036893-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR036893-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="199" mass="22774" checksum="F9CF1DADBF290C21" modified="2018-12-05" version="1">MLRFVVALCLLALSWAQDCQVENFQVKQDFDKTRYAGTWYAVGKKDPEGLFLLDNVVAQFNIEEDGSMTATAKGRVIILNNWEMCAHMFATFEESPNPAKFRMRYWGAASYLQTGNDEHWVIDTDYDNYALHYSCRLLNEDGTCLDSYSFVFSRHPAGLRPEDQALITQKKTDLCLLGKYRRVTHNGFCENSGNAEPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDN8</accession>
    <name>A0A3B5LDN8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="10">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:15842"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDN8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008690"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008796.1">
        <property type="protein sequence ID" value="ENSXCOP00000008690.1"/>
        <property type="gene ID" value="ENSXCOG00000006636.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063435"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000013">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000111">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="6 10" id="KW-0521">NADP</keyword>
    <keyword evidence="7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="11">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="12">
        <location>
            <begin position="165"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="468" mass="53525" checksum="E3779885D54C928E" modified="2018-12-05" version="1">IYPTLWWLFKDGLLPEDIYIVGFARSDLTVDKIKAACLPHMKDTDKELDSLSAFFKRNSYLSGRYDVGSSFERLNSHLSSLPGGETANRLFYLALPPTVYHHVSTNIRNHCMSCKGWNRVIFEKPFGHDLQSSQELSAHLANLFKEDQIYRIDHYLGKEMVQNLMVLRFGNRLFGPIWNRNSVACVVLTFKEPFGTQGRGGYFDGFGIIRDVMQNHLLQMLCLIAMEKPTSTSPDDVRNEKVKVLKCIAPPNMADVVLGQYVGDPGEEGDANLGYLDDPTVPKGSCTPTFATAVLYVQNERWDGVPFILRCGKALNERKAEVRLQFTDVPGDIFADRCQRNELVVRVQPDEAIYLKMMSKRPGVFFGPEETELDLTYKSRYKIVKLPDAYERLILDVFCGNQMHFVRSDELREAWRIFTPLLHQIEAAKRQPIPYTYGSRGPSESDDLIQRVGFRYEGTYKWVQPQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEK9</accession>
    <name>A0A3B5LEK9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma</fullName>
            <ecNumber evidence="14">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:33180"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LEK9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010603"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010727.1">
        <property type="protein sequence ID" value="ENSXCOP00000010603.1"/>
        <property type="gene ID" value="ENSXCOG00000008017.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158901"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010634">
        <property type="term" value="P:positive regulation of epithelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13362">
        <property type="entry name" value="PH_PLC_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08592">
        <property type="entry name" value="PI-PLCc_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09932">
        <property type="entry name" value="SH2_C-SH2_PLC_gamma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10341">
        <property type="entry name" value="SH2_N-SH2_PLC_gamma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11970">
        <property type="entry name" value="SH3_PLCgamma1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000155">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000051">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000004">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000007">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000009">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000011">
        <property type="entry name" value="1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056586">
        <property type="entry name" value="EF-hand_PLCG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016279">
        <property type="entry name" value="PLC-gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035023">
        <property type="entry name" value="PLC-gamma_C-SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035024">
        <property type="entry name" value="PLC-gamma_N-SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057061">
        <property type="entry name" value="PLCG_EF-hand_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035724">
        <property type="entry name" value="PLCgamma1_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF173">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE GAMMA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23329">
        <property type="entry name" value="EF_HAND_1_PLCG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23583">
        <property type="entry name" value="EF_HAND_2_PLCG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000952">
        <property type="entry name" value="PLC-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 14 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 14 18" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="10 14 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 16" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="2 17" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="11 14" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PH" evidence="22">
        <location>
            <begin position="26"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="25">
        <location>
            <begin position="150"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="20">
        <location>
            <begin position="548"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="20">
        <location>
            <begin position="666"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="21">
        <location>
            <begin position="789"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="22">
        <location>
            <begin position="870"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="domain" description="PI-PLC Y-box" evidence="24">
        <location>
            <begin position="951"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="23">
        <location>
            <begin position="1069"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1215"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="1215"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="15">
        <location>
            <position position="1212"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="15">
        <location>
            <position position="1237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR000952"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
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        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1266" mass="145241" checksum="0534B3636918CF98" modified="2018-12-05" version="1">MAGTSGFLSNGPVPWMDDTEMNNLYRDLELGTVMTLFYSKKSQRPERRTFQVKLETRTIIWTRSTDKIEGEIDIREIKEIRPGQKSRDFERYVEDSAVRLDNFCFVILYGTEFRLKSLSLAATSDEEMSMWVKGLKWLRDDTLKSPTPLQIERWLRKQFYAADRNREDRISYKDLKNMLSQVNYKVPNMRFIKDKLPDSETRNGDVTFSQFAQLYRNLMFDAQKNVSADILCAPITLPLLRSSAEAHFIPHLCVLKWLQDQYELWASDNNKVQEFMFSYLKDPLREVEDPYFHPEEFLTYLFSKENTIWDSSLDMVCAENMNNPLSHYWISSSHNTYLTGDQFASESSLEAYARCLRMGCRCIELDCWDGPDSMPVIYHGHTLTTKIKFNDVLNTIKDHAFVTSEYPIILSIEDHCSIVQQRNMATQFKKVFGDMLLTKAVDMAADGLPSPNQLKKKILIKHKKLAEGSAYEEVPTATPYSENDISNSIKNGILYLEDPINHDWYPHFFVLTHNKIYYSEETTNNVGNDEDEELKEVSTSKGHVTEKWFHGKLGAGRDGRQIAERLLSDYCQETGAPDGSFLVRESETFVGDYTLSFWRLGRVQHCRIHSRQEAGSPKFYLTDNLVFDSLFALITHYQHVALRCNEFEMKLTEPVPQTNAHESKEWYHANLSRSQAENMLMRVPRDGAFLVRKRTEPNSFAISFRAEGKIKHCRVLQEGQVVLLGTSEFDSLVDLISYHEKHPLYRKMKLRYPINDDTLEKIGTAEPDYGSLYEGRNPGFYVEANQMPTLKCTVKAMYEYKAQRDDELSFSKNAIITNVDKQEGGWWKGDCGGKKQMWFPANYVEEISQAAAEPDRTEITENSPLGDLLRGSVDVTSCQIITRTEGKGSRPYVFSLVQNNTVQGTLLDVAANSAEELQDWKTKIQEVAATSEAKLEEGKIMERRKKIALELSDLVIYCRPVPFDEEKIGTERACFQDMSSFPETKAEKYVNKTKGKKFLQYNRLQLSRIYPRGQRLDSSNYDPFPMWLCGSQLVALNFQTADKPMQMNQALFMLNGKSGYVLQPPIMRDDTFDPFDKNTLRGVDQVSMKIEVLGARHLPKHGRGIVSPLIEIEVCGADFDNDKQKTESAADNGLNPTFPRKSFSFTVCNSAFAFLRFGVYEIDMFNDQNFLAQATFPIQSLKTGYRSVPLKNSYSEDLELASLLDENGEVLSPFSAAGSVSAPSPSQAMSYRGREGSFESRYQSPIDDFRVSQEALLDQMDPQNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFF1</accession>
    <name>A0A3B5LFF1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cystatin fetuin-B-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFF1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008411"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008517.1">
        <property type="protein sequence ID" value="ENSXCOP00000008411.1"/>
        <property type="gene ID" value="ENSXCOG00000006452.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182930"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025764">
        <property type="entry name" value="Cystatin_Fetuin_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050735">
        <property type="entry name" value="Kininogen_Fetuin_HRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001363">
        <property type="entry name" value="Prot_inh_fetuin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13814">
        <property type="entry name" value="FETUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13814:SF15">
        <property type="entry name" value="SI:CH211-262H13.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51530">
        <property type="entry name" value="CYSTATIN_FETUIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01254">
        <property type="entry name" value="FETUIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Cystatin fetuin-B-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="251"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="329"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="374" mass="40900" checksum="12298CD3CF2E8523" modified="2018-12-05" version="1">YWMITKLAPVPCNDKAVEKLSRLAVTYINEDRTEGYKFALNRIANVYLHAQGPGGNVYYLDLDVLETKCHIGSPKPWKRCDVRPFMETQISGNCNTTILHTPEGFSYLYSYDCALVPDPPEKLLQTCPTCPVLLSVDSPLAMDTAGITLEAYKRQSTLGAGLGVKRVTRAAAQVPQWLMLAGNESLLLNSLLNNVLSTAGFCAGSVYGDVSKYPDVQVSCEMYKIQVHDLPTDTMIPTFPPLPDDPLIDLQPIPSDPLTPDVAQPALIDPKATPSLPTDPPVVANPSVLLSSSSESAERIVQQNTGTLDSSSEEIGVLVARRPPFNFRYPRRKRDKRQSLTETSPSHSPVFLSDFPSGSSPFRSCPGPARYATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFU3</accession>
    <name>A0A3B5LFU3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Pre-mRNA-splicing factor 38A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 9">Involved in pre-mRNA splicing as a component of the spliceosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the spliceosome B complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the PRP38 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFU3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009722"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009836.1">
        <property type="protein sequence ID" value="ENSXCOP00000009722.1"/>
        <property type="gene ID" value="ENSXCOG00000007393.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111085"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071005">
        <property type="term" value="C:U2-type precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005037">
        <property type="entry name" value="PRP38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024767">
        <property type="entry name" value="PRP38_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23142">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 38A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03371">
        <property type="entry name" value="PRP38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12871">
        <property type="entry name" value="PRP38_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor 38 C-terminal" evidence="11">
        <location>
            <begin position="178"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="181"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="224"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="289"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="299" mass="35755" checksum="E0BDC89BFC219C70" modified="2018-12-05" version="1">MANRTVKDANSIHGTNPQYLVEKIIRTRIYESKYWKEECFGLTELVVDKAMELKFVGGVYGGNIKPTPFLCLTLKMLQIQPEKDIIVEFIKNEDFYVRLLGAMYMRLTGTAVDCYKYLEPLYNDYRKIKSQNRNGFELMHVDEFIDELLHAERVCDIILPRLQLQKRQVLEEAEMLDPRVSALEEDLDEVESSEEEDEEEEKFERLPSPEPHRRGHRDNDRPRRSPSPRYRRSRSPRRSRSPKRRERHRSKSPRRHRSRSRDRRHRSKSPHHRSHRHRSHSKTPESSKKSHKKSRRGND</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGF2</accession>
    <name>A0A3B5LGF2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGF2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010050.1">
        <property type="protein sequence ID" value="ENSXCOP00000009932.1"/>
        <property type="gene ID" value="ENSXCOG00000007529.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182774"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002325">
        <property type="entry name" value="Si:cabz01021430.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001480">
        <property type="entry name" value="Si:cabz01071911.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001639">
        <property type="entry name" value="Si:dkey-7i4.21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000966">
        <property type="entry name" value="ZFP90 zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001370">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001156">
        <property type="entry name" value="Zinc finger protein 407"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000213">
        <property type="entry name" value="Zinc finger protein 624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF151">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="85"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="253"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="281"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="365"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="393"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="420" mass="48097" checksum="7D177AF9644245F2" modified="2018-12-05" version="1">MQNEVEYQQSKEREKKLCISQDEEQLVLKPETDDILVNPSNVQKILNETQPQTNQLISTEEQKQNKRFKSSLTRHSMIHTNENRFSCTSCGKKFFSKILLASHTKTHKYIKSFLCETCKKRFRTKSQLLIHIRTHTGEKPFSCMTCGKGFARRASLTYHMMTHTGEKPFSCGTCGKCFLTKFQLLLHMRIHTDEKPFCCVTCGKSFSRKHILNVHMRTHSGEKPFSCVACGKCFSRKDTLNVHMRFHTGEKPFSCVSCGKSFLLKMHLLNHMTIHVGEKPFSCGTCGKSFINKGHLNVHMRIHTGEKPFSCGTCGKSFTLKQTLDHHMKIHTDQKSFSCGTCGKSFRLKMYLLNHMRIHTGEKPFSCGTCGKSFGRKMHLLNHVRIHTGEKPFSCGTCGKSYSHRSSLTHHMKTHSDKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIM5</accession>
    <name>A0A3B5LIM5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Regulator of chromosome condensation (RCC1) and BTB (POZ) domain containing protein 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027854805.1">
        <property type="nucleotide sequence ID" value="XM_027999004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIM5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007724"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007821.1">
        <property type="protein sequence ID" value="ENSXCOP00000007724.1"/>
        <property type="gene ID" value="ENSXCOG00000005937.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114133261"/>
    <dbReference type="KEGG" id="xco:114133261"/>
    <dbReference type="CTD" id="1102"/>
    <dbReference type="GeneTree" id="ENSGT00940000158925"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd18529">
        <property type="entry name" value="BACK_RCBTB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18354">
        <property type="entry name" value="BTB_POZ_RCBTB2_CHC1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051625">
        <property type="entry name" value="Signaling_Regulatory_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872">
        <property type="entry name" value="BTK-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872:SF3">
        <property type="entry name" value="RCC1 AND BTB DOMAIN CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="41"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="94"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="147"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="200"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="252"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="370"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="527" mass="57600" checksum="F809061A6210B8A9" modified="2018-12-05" version="1">MLDVGKWPVFALLPPEELRLIRQACVFGSAANEALYVTVNDEVFALGTNCSGCLGLGDLQSTIEPRRIDILCGKKIVSLSYGTGPHVVIATADGEVFAWGHNGYSQLGNGTTNHGLTPALVSTNLISKRVTEVACGSHHTIALTSDGEVYAWGYNNSGQVGSGSTANQPTPRRVSSCLQNKVVVNIACGQLCSMAVLDNGEIYGWGYNCNGQLGLGNNGNQQTPCRIAALQGVNIVQVACGYAHTLTLTDEGFVYAWGANSYGQLGTGNKSNQALPILINTDKERMVEVAACHTSHTSAAKTQSGQVLMWGQCRGQAVSSPHLTHFSSTDDVFACFATPAVTWHLLSVDGDDYLTVAQSLKKEFDSPEISDLKFLVDGKCIYVHKALLKIRCEHFRTLLNETDEDVIEIHQFSYLVYRAFLEYLYTDNINLPPEDAIGLLDLATFYREMRLKRLCQETIKRGISEENAITLLSAAVKYEARDLEEFCFKFCVNHLTAVTQTQAFADMDHDLLKNFISKASRYGAFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJX4</accession>
    <name>A0A3B5LJX4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Galectin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJX4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009911"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010029.1">
        <property type="protein sequence ID" value="ENSXCOP00000009911.1"/>
        <property type="gene ID" value="ENSXCOG00000007520.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165169"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001772">
        <property type="term" value="C:immunological synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048030">
        <property type="term" value="F:disaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019863">
        <property type="term" value="F:IgE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043236">
        <property type="term" value="F:laminin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048245">
        <property type="term" value="P:eosinophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048246">
        <property type="term" value="P:macrophage chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002548">
        <property type="term" value="P:monocyte chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045806">
        <property type="term" value="P:negative regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001237">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030593">
        <property type="term" value="P:neutrophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050918">
        <property type="term" value="P:positive chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090280">
        <property type="term" value="P:positive regulation of calcium ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00070">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000023">
        <property type="entry name" value="Galectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044156">
        <property type="entry name" value="Galectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346:SF26">
        <property type="entry name" value="GALECTIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00276">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0221">Differentiation</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0389">IgE-binding protein</keyword>
    <keyword evidence="13" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="10 19" id="KW-0430">Lectin</keyword>
    <keyword evidence="8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="17" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Galectin" evidence="21">
        <location>
            <begin position="150"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU102079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="285" mass="31020" checksum="098D1A378BF86CD6" modified="2018-12-05" version="1">EKNNPFCPPSGSAPQSGGLWPTLPPSGAGFPAWPGQPTQPVPCWTGQPNTACWPAPQPAPVSVPAPTQVITPPAPTATLVPAPVQVPTSAVQQPCQPSPAAATIHPGLPGWPAHPGWPYNPGQSGWPGQNPSMMPPHWLPPTSGPMSVPYNLNLARGVYDKMMMTILGYVKPNAKMFTVNFLRGNDIAFHINPRFNEGGKQVVVRNHKLGDRWGPEERDLKGPFPFAPGSPFEMKILCTPETFRVAVNNIPLFEFRHRIRELNQIDRINILNDVVLTYVNVETLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LK60</accession>
    <name>A0A3B5LK60_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cyclin-dependent kinase inhibitor 1C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cyclin-dependent kinase inhibitor p57</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">p57Kip2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with PCNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDI family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LK60"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011040"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011167.1">
        <property type="protein sequence ID" value="ENSXCOP00000011040.1"/>
        <property type="gene ID" value="ENSXCOG00000008341.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162677"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045930">
        <property type="term" value="P:negative regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.365.10:FF:000002">
        <property type="entry name" value="cyclin-dependent kinase inhibitor 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.365.10">
        <property type="entry name" value="p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003175">
        <property type="entry name" value="CDI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044898">
        <property type="entry name" value="CDI_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10265">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10265:SF44">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02234">
        <property type="entry name" value="CDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-dependent kinase inhibitor" evidence="13">
        <location>
            <begin position="31"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="92"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="104"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="195"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00078591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="219" mass="24625" checksum="959CC08B814BBD68" modified="2018-12-05" version="1">MSHVQLSSSALERLVARRTFPLQRRTGVCRSLFGPVDHDELSRDVKAKLREISERDQQRWNFNFEDNTPLEGDYEWEEMPAERTAAFYRESVQSGRTRVPATPVKQTPFSESVMSESSDGDVMERLAVPESDRSVVGGDGESSSPTPAEVNQENRPVTGISGTRRQAPRVRRKRSAAAAADDNNTHITGSYRGKQPREAADRLSKSPISLEQTPRKRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKZ6</accession>
    <name>A0A3B5LKZ6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">nucleoside diphosphate phosphatase</fullName>
            <ecNumber evidence="15">3.6.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Guanosine-diphosphatase ENTPD5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Uridine-diphosphatase ENTPD5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Hydrolyzes nucleoside diphosphates with a preference for GDP, IDP and UDP compared to ADP and CDP. In the lumen of the endoplasmic reticulum, hydrolyzes UDP that acts as an end-product feedback inhibitor of the UDP-Glc:glycoprotein glucosyltransferases. UMP can be transported back by an UDP-sugar antiporter to the cytosol where it is consumed to regenerate UDP-glucose. Therefore, it positively regulates protein reglucosylation by clearing UDP from the ER lumen and by promoting the regeneration of UDP-glucose. Protein reglucosylation is essential to proper glycoprotein folding and quality control in the ER.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>ADP + H2O = AMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61436"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:61437"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>CDP + H2O = CMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:64880"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="3.6.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:64881"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>GDP + H2O = GMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22156"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:22157"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>IDP + H2O = IMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35207"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58280"/>
            <dbReference type="EC" id="3.6.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:35208"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>UDP + H2O = UMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:64876"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="3.6.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:64877"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a ribonucleoside 5'-diphosphate + H2O = a ribonucleoside 5'-phosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36799"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.6.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:36800"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Monomer; active form. Homodimer; disulfide-linked. Homodimers are enzymatically inactive.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.6" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKZ6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012758"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012912.1">
        <property type="protein sequence ID" value="ENSXCOP00000012758.1"/>
        <property type="gene ID" value="ENSXCOG00000009636.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286963"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017110">
        <property type="term" value="F:nucleoside diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030282">
        <property type="term" value="P:bone mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055062">
        <property type="term" value="P:phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000052">
        <property type="entry name" value="Ectonucleoside triphosphate diphosphohydrolase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF35">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE PHOSPHATASE ENTPD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="active site" description="Proton acceptor" evidence="26">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <begin position="201"/>
            <end position="205"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00046723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00049328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="424" mass="47922" checksum="A31656353413B6F5" modified="2018-12-05" version="1">VPEVFHPAPEVVHTIPEAFHPVPEVVHPAPETHHVLQVPDRNRDSRVFYGIMFDAGSTGTRIHIYKFIQKDPELPVLDNEMFHAVKPGLSEYKDNPEEGGNTIRQLLKIAKKTVPEEEWRRTPVVLKATAGLRLLPLEKANALLKELQVQEVFEESPFYVPENSVTIMNGANEVLAWVTVNFLTHLYSNTRRTVGILDLGGGSTQITFLPKSKQKTIEVAPRSFTAKFNLFNHTYELYTHYLGNGLVAARLATLGALGADLDWKVFTSSCLPKKYREEVKFGGDSYKVSGIPGYAGYKLCYYEVMRVVKGNFHQPYEVKGSSIFYAFSHYYDRAVQTGLVSLGGAVEVRDFKKKAKECNKMTKYRAISPFLCMDMTYITCLLKEGFGFKDSTVLHLAKKVNNVETSWALGATFDFFRNFNINQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPD7</accession>
    <name>A0A3B5LPD7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPD7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012862"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013018.1">
        <property type="protein sequence ID" value="ENSXCOP00000012862.1"/>
        <property type="gene ID" value="ENSXCOG00000009725.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004097"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016581">
        <property type="term" value="C:NuRD complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040386">
        <property type="entry name" value="P66"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032346">
        <property type="entry name" value="P66_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13455:SF4">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR P66-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13455">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR P66-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16563">
        <property type="entry name" value="P66_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="8">
        <location>
            <begin position="409"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="111"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="266"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="267"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="589" mass="64376" checksum="6DF6624C321D5361" modified="2018-12-05" version="1">MERMSDDTLRLNLLKRGLESSSEREEALSKRLKMEGHEAMERLKMLALLKRKDLADLAALEVPGTLGDGKGPSEKLNGSLRLGSHSVHAGQSKNGKENIMDEPVDMSAGRRCEVDRDRQTPSPDVIILSDNEASSPRTTPRPEERMHQANLEIFKGKTGEERQQMIKALREELRLEEARLVLLKKLRQSQMQKENVVQKVPAVQSSASSVQPPPIHSSSGIGKLPVRPGLHNTEPQNLRTAQGHTVIRSANTSLPPMLMSQRVIAPSPSQLQGQRVSSKPGMPRSSSSSMTSAVNYQQASQQVAASQRSSSSAMYMNLAHMQAAAAVGASGVTVSSMADQASSQAAAKLALRKQLEKTLLEIPPPKPPAPLLHFLPSAANSEFIYMVGLEEVVQSVLDSQGKFRGSLARMEPFFCAQCRTDFTPHWKQEKSGRILCEQCMTSNQKKALKAEHTNRLKNAFVKALQQEQEIEQRLQQQAALSPSSAATGSNVSKTDTMIRQHALRQAQQPQASLQRGLSNSARGVLSNFAQASQLSVASSLMGMSRLNIAYLNPAAVGAHKTSSLADRQREYLLDMIPPRSISQSITGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRK9</accession>
    <name>A0A3B5LRK9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Gamma-glutamyltransferase 5a</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-glutamyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRK9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010429"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010552.1">
        <property type="protein sequence ID" value="ENSXCOP00000010429.1"/>
        <property type="gene ID" value="ENSXCOG00000007901.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155794"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002951">
        <property type="term" value="F:leukotriene-C(4) hydrolase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901750">
        <property type="term" value="P:leukotriene D4 biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.130:FF:000001">
        <property type="entry name" value="Gamma-glutamyltransferase 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686">
        <property type="entry name" value="GAMMA GLUTAMYL TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686:SF19">
        <property type="entry name" value="GLUTATHIONE HYDROLASE 5 PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="361"/>
            <end position="363"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="401"/>
            <end position="402"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="515" mass="56310" checksum="295E901BCC03E9B8" modified="2018-12-05" version="1">MWVTFFTTKKKNPTRSSNRCEFSRDILKGGGSAVDAAIAALLCTGIMNPQSAGVGGGAIFTVMDRSGKVKVINSRETAPSKVDPDLLKSCKNESIPRTGSKWIGVPGEIRGYELAHKLYGKLPWAALFQPTIKLAREGFPIPPVQGSGITDNRMKNTSLRKLYLDKTGTLLKTGDEIKFEKLADTLELIANKGADAFYTGRVAEDLVRDVQQEGIAFRDLASYKATVTEAWVAPVGEYQMYIPPPPSGGPLLSLVLNIMKGYQMNSAPRSVDEKILFYHHYVEALKFANGQKKQVRDNLIPFKLAKNMTSSGFADELRLRIRDDRTNSPVYYNFNKYPDSMGTTHVSVLAEDGSAVAVTSSINYKFGSKVYSSSTGVLLNNQLLDFCETANSVSPGEQPPSSMTPVVLKSKSKTLVIGAGGGRWITTGLASVLINHLWFGKSLKEAIDTPVVYVDSQNTVKFESSLHKGVIEALEALGHNQTSEEFYNVVNAVEKKDDYICAVSDDRKRGEAAGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LS30</accession>
    <name>A0A3B5LS30_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="EEIG1/2-like"/>
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            <begin position="24"/>
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            <begin position="38"/>
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            <begin position="84"/>
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            <begin position="140"/>
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        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="228" mass="24330" checksum="6F944E8D4489F0E3" modified="2018-12-05" version="1">MTLLSGDPCFKTAPSTAKSISIPGREHTLQLDCKGEGTAEGTGPAGGVSLGRASKPRPSIISSGLLDDSELNQNQSGPGEVFQSGHSRNSSYASQQSKISGYSTEHSCSSSLSDLTHRRNTSTGSSASGGLCFTADPPPEGDKDAGRPERPPRPPRPVLPPNRPLRRKQDSVESHPTWVNDTRINADDIVEKIVQSQNFADINNTEGKEAAFLWLEPQRSFSSVCNTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSN4</accession>
    <name>A0A3B5LSN4_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">WH2 domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Xiphophorus couchianus</name>
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                <consortium name="Ensembl"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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            </authorList>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <end position="53"/>
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            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
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            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="424" mass="42868" checksum="68E2B499C744D012" modified="2018-12-05" version="1">DRMPIPPPPPPGPPPPPTFSQANTTPPKLNSSETKGRGALLSDICKGAKLKKVAEVNDRSAPVLDKPGGGGGGGGGGGGFGGGGPVGMGALFQGGVPKLRPVGAVGSVGRSALRPPGSRPAAPRPPGGRSTSPTPANKPSSSDHQRSHRPSLPDISRPPGNNSSSGGMKHSTSAPPPPPPFSKGSRGNAPPTPNQKGPSYSRDKVLPPTPTRGSSSSSSSSSSSNVKSNHSSSKPPSSGSSMPPPPPPYRPSGTANGPSQVDGGGAPELPQRKNSLRNKRTSGGSGGGGQGRNHAPPPPPVPPSSSQDAPPPPPYRGHSTGVSEQHGRVKPPPPPSPSPVSSSSSSRTPAGPPPPPPPFRNGHSTDDFESKFNFHPIEDLPPPEEFRPSNRVYPSKSDRGTSALTVQLLQFNIYSAAAARRAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSP8</accession>
    <name>A0A3B5LSP8_9TELE</name>
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        <submittedName>
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        </submittedName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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            <taxon>Poeciliinae</taxon>
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        </lineage>
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    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="term" value="P:cellular response to xenobiotic stimulus"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:chordate embryonic development"/>
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        <property type="project" value="Ensembl"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF2_snRNP_like_II"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Translation elongation factor 2"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Translation elongation factor 2"/>
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    </dbReference>
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        <property type="entry name" value="Translation elongation factor 2"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
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        <property type="entry name" value="EFTu-like_2"/>
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        <property type="entry name" value="G_TR_CS"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    </dbReference>
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        <property type="entry name" value="Small_GTP-bd"/>
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        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
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        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
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        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ELONGATION FACTOR 2B-RELATED"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
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        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EFG_IV"/>
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    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
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        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="5"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="846" mass="94130" checksum="EC7369EC4B84A5A5" modified="2018-12-05" version="1">MDKKSNIRNMSVIAHVDHGKSTLTDSLVSKAGIIASARAGETRFTDTRKDEQERCITIKSTAISLYYELEENDLAFIKQSKDGSGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLEPDELYQTFQRIVENVNVIISTYGEDEGGPMGSIMIDPVIGTVGFGSGLHGWAFTLKQFAEMYVAKFAAKGATQKGPAERCKKVEDMMKKLWGDRYFDPSAGKFSKTASGPDGQKYPRTFCQLVLDPIFKVFDAIMNFKKEETAKLIDKLDVKLDTEDKEKEGKPLLKAVMRRWLPAGEALLQMITIHLPSPVTAQKYRCELLYEGPGDDEAAMGIKSCDSKAPLMMYISKMVPTSDKGRFYAFGRVFSGCVSTGLKVRIMGPNFTPGKKEDLYIKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEQAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPLKKSDPVVSYRETVTEESDQMCLSKSPNKHNRLFMKSRPFPDGLAEDIEKGEVTARQELKARARYLAEKYEWEVTEARKIWCFGPDGTGPNLLIDITKGVQYLNEIKDSMVAGFQWATKEGALCEENMRAIRFDIHDVTLHTDAIHRGGGQIIPTARKVLYACQLTAQPRLMEPVYLVEIQCPEQVVGGIYGVLNKKRGHVFEESQVIGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPSDVTSKPFQVVSEIRKRKGLKEGIPALDNYLDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTL5</accession>
    <name>A0A3B5LTL5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Hypoxia inducible factor 1 subunit alpha, like</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTL5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012671"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012825.1">
        <property type="protein sequence ID" value="ENSXCOP00000012671.1"/>
        <property type="gene ID" value="ENSXCOG00000009417.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166085"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990907">
        <property type="term" value="C:beta-catenin-TCF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048821">
        <property type="term" value="P:erythrocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001654">
        <property type="term" value="P:eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051402">
        <property type="term" value="P:neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014823">
        <property type="term" value="P:response to activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000114">
        <property type="entry name" value="Hypoxia inducible factor 1 subunit alpha, like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000015">
        <property type="entry name" value="Hypoxia-inducible factor 1-alpha isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021537">
        <property type="entry name" value="HIF_alpha-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043:SF34">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 SUBUNIT ALPHA,-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23171">
        <property type="entry name" value="bHLH_HIF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11413">
        <property type="entry name" value="HIF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14598">
        <property type="entry name" value="PAS_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BHLH" evidence="12">
        <location>
            <begin position="45"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="11">
        <location>
            <begin position="113"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="11">
        <location>
            <begin position="281"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="686" mass="76755" checksum="B4F6C1763C7CB8E4" modified="2018-12-05" version="1">MVGVSVCVLWSGPVQTEAQKLQSSLGFLQKEEEEEGAGSRSSSEQRKLRSRDAARCRRSQETEVFYELARTLPLPRRVCTHLDKAGIMRVTLSFLRMQQLLQPEESVKKEEEDEDPMDAFFPQALAGFVMVMTEEGDMVFLTENVNKHIGITQLELLGQSVYDFIHPCDQEELRDLLTPRPGLSKKLMAEQPSERNFFLRMKSTLTSRGRTVNIKSATWKVLHCTGHIRPFGSASPPAARVMTLLCEPIPHPSSVEFPLDSCTFLTRHTMDLRFTHCEGRVTELVGYKPEDLIGRSAYEFYHALDSDRIKKSLLTLLSKGQANSSPYRFLANCGGFVWAETQATVLYSKTSQPEAIVCLNFILSAVEQPDVVFSVEQTRCSLLPKTEPSSLERAAEDSGISDTCDSDGEDAGSSAKLLLKLAEKPEELLQLPDAAGFSPSGRVELSFASPPSPDVVPDHPKDLCSPQLRQLLSPIFDGLNPSSSPAASSEPDLSPCEEEVMDTSEVERFFTICPEETQLKEQAIEDVEGKELEMYAPYISMDDDFQLTFLSNLPQEANKPASLLFQGADGIPAVTVSRKRTHDLDKEPPPLLMIQDKRHKPDPSSIEEELLLSNRLLVRLKVHRRSKQFKLKLGIFNHFVSAIKHFKVFFCPSYLKQNRLCVSAALMRDIFVPRPADLSPPLSPMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVH3</accession>
    <name>A0A3B5LVH3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Unc-93 homolog B1, TLR signaling regulator</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the unc-93 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVH3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015958"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016157.1">
        <property type="protein sequence ID" value="ENSXCOP00000015958.1"/>
        <property type="gene ID" value="ENSXCOG00000012055.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063359"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035325">
        <property type="term" value="F:Toll-like receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034121">
        <property type="term" value="P:regulation of toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034138">
        <property type="term" value="P:toll-like receptor 3 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034154">
        <property type="term" value="P:toll-like receptor 7 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034162">
        <property type="term" value="P:toll-like receptor 9 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002224">
        <property type="term" value="P:toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010291">
        <property type="entry name" value="Ion_channel_UNC-93"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043268">
        <property type="entry name" value="UNC93B1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46744">
        <property type="entry name" value="PROTEIN UNC-93 HOMOLOG B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46744:SF1">
        <property type="entry name" value="PROTEIN UNC-93 HOMOLOG B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05978">
        <property type="entry name" value="UNC-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="463"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="508"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="512"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="547"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="586" mass="67397" checksum="41BEFD36D1A053E4" modified="2018-12-05" version="1">MEEFLGPQAQYNEEEEERKYYRRKRLGVIKNVVASSFGAMIVYSVYMGLLQMQLTLHYDMTYREVKYSNLGLVDIDRKMLMGINVTPIIGLLYTPVLIRFLGTKWMMFLASGIYALFVSTNYWERYYTLVPSAVVIGVAIVPFWASLGSYITRMAQQYYEYANYKEEHVQEQRKLPKGACYSYIITFQSIFYVIFNLSFVFAEFPMRIVLHDHLHENYHVLCNVKTCGAAIKGIIPGFNSTVLKNLPRSMLLIEVESILMGFAFLAMITFLLLCGPAYRPTEEIDLRSIGWGNIFQLPFKHLRDYRLRLLCPFFIYSGLEMLFIISGFSLSYGVCILGIKTLWLLILVYGLSCSVFSLLSLSLLRLPRWVCLMGGAAVHGVLLVVLLALTVKPNSPEYLGPLLVIMVLWGLGSALNKTGVSTVVGLLYAEEKERLDFVYTIYHWWQAIAIFVVYLWSQMPMRAKLSILLATLLLACYCYWVMERRVAQKVKPRMPRIPRPRHKVKGYRYLEEDNSDESDSEGSEEDDDEDEEERTEEDERVVEEMVAGDREEEGRDARAEGADSPRAWRRGAEGQGAHVEDEGREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVJ0</accession>
    <name>A0A3B5LVJ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GCF C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVJ0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011849"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011985.1">
        <property type="protein sequence ID" value="ENSXCOP00000011849.1"/>
        <property type="gene ID" value="ENSXCOG00000008965.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011802"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024855">
        <property type="entry name" value="UNC79"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21696">
        <property type="entry name" value="PROTEIN UNC-79 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21696:SF2">
        <property type="entry name" value="PROTEIN UNC-79 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14776">
        <property type="entry name" value="UNC-79"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="17102" checksum="19632328323F5DBC" modified="2018-12-05" version="1">MVFQWFHSTAYMMDDEVGSLVEKLKPQFVTKWLKTVCEVRFDVMVMCLLPKPVEFARVGGYWDKSCSKVTQLKEGLNRILCLIPYNVISQPLWECFMPEWLEAIRTEVPDNQLKEFREVLRYKLGYLHWNLDPKNLVRFCFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LX68</accession>
    <name>A0A3B5LX68_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Glutamate--cysteine ligase</fullName>
            <ecNumber evidence="3 10">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Gamma-ECS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 10">Gamma-glutamylcysteine synthetase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteine + L-glutamate + ATP = gamma-L-glutamyl-L-cysteine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glutamate--cysteine ligase type 3 family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="3 10"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LX68"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015747"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015941.1">
        <property type="protein sequence ID" value="ENSXCOP00000015747.1"/>
        <property type="gene ID" value="ENSXCOG00000011897.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011908"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017109">
        <property type="term" value="C:glutamate-cysteine ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="Glutamate--cysteine ligase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000003">
        <property type="entry name" value="Glutamate--cysteine ligase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004308">
        <property type="entry name" value="GCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164">
        <property type="entry name" value="GLUTAMATE CYSTEINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164:SF0">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03074">
        <property type="entry name" value="GCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="4 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="581" mass="66462" checksum="9BDD37FED9DE3505" modified="2018-12-05" version="1">MGLLSQGSPLNWEETRKYADHIRKHGIIQFLNIYNKVKDRQRDVLKWGDEVEYMLVELDDNDEKVRLVLNGGDVLETLQEQGENTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEGNMRKRRLEASSVLKKNMTLCTITSFPRSGCPGFTKPEYRPTPVEKGVSKSLFFPDEAINRHPRFSTLTRNIRHRRGEKVVINVPIYKDKHTPSPFVEQFPEDDGEAARAALPDHIYMDAMGFGMGNCCLQVTFQACSIDEARYLYDQLATFCPIVMALSAASPFYRGYMSDIDCRWGVISASVDDRTQEERGLEPLKNNKYRIFKSRYDSIDSYLSCCGEKYNDIDLTIDEEINKQLLSAGIDKLLAQHIAHLFIRDPLSVFEEKIHLDDENESDHFENLQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENAAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRNAVQEGMFYFRKDMFKGCNPGLDCASSAQNGVEFEGSNEDYTLMSIDTIINGKVSLFETKPESDEFISSLLCQSSVLHLHKQSQLATLSLRYMKGKCCKSIHTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LY91</accession>
    <name>A0A3B5LY91_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Adhesion G protein-coupled receptor G7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LY91"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014488.1">
        <property type="protein sequence ID" value="ENSXCOP00000014311.1"/>
        <property type="gene ID" value="ENSXCOG00000010854.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159169"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053066">
        <property type="entry name" value="ADGR_G7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47767">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR G7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47767:SF1">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR G7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="338"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="374"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="429"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="473"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="502"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="GAIN-B" evidence="7">
        <location>
            <begin position="88"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="8">
        <location>
            <begin position="238"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="577" mass="65019" checksum="0B91B5067D852191" modified="2018-12-05" version="1">TTTTTTTTTTTTTTTSTKPPLICANGHNESGVCVCPDEWTGKNCSEGESCWCLCLPPLRTEELFVSWFMSPWTRILSLTRWFTSASHQVGSLHQRTYNLLLATADPRFVPLHPGRGAERRQQGSNVSLGFVLYQNSRFFPSRIYRRRRASLRVLSASIGGPDRSVVAETVEMQFRLPRNTSLHDFSCVFWDYGKQDWNTHGCHKGNASDGVLRCSCNHTTNFAALWSFRDDYKYAEPLMRISIVGLSFSILGLVITIIHHLKETFQMKSGQKQTKLNSKTSLLCICFSLLAFIITFLSGVQNLRQDYDTKEPPSDQNQILDSEKSVVPDSGSCTAVTALLHFFLLATFSWNSLYGTQVVLLVRSMQRSLPSYWTGLSWGVGWGVPAVVMAITLATTYRVENPLAYRQEEFCWLAALDQDKDFSFGKPMFWAFLVPLGLVLLYNTVLLGLVSLTTCRVDRKLTSTKRSSLAQKFLVSFSLAVLLGLSWTLGYLVLVTEETAHLVWSILFCLCTTTQGLQIFILFTARTRSFRVAVGRSAHYVSVAGFTLRSTTYYLWKNRAETRTSDMYNTKDRDTSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYI0</accession>
    <name>A0A3B5LYI0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein ABHD15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Alpha/beta hydrolase domain-containing protein 15</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May regulate adipocyte lipolysis and liver lipid accumulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with PDE3B; this interaction regulates PDE3B's stability and expression and, thereby, impacts the antilipolytic action of insulin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYI0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016953"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017172.1">
        <property type="protein sequence ID" value="ENSXCOP00000016953.1"/>
        <property type="gene ID" value="ENSXCOG00000012782.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182902"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034338">
        <property type="term" value="F:short-chain carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000103">
        <property type="entry name" value="Abhydrolase domain-containing 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF78">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING 15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00082877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="496" mass="54636" checksum="89C57AC065B6C692" modified="2018-12-05" version="1">MLEWLVAFCVVILVLFIWPGTKYLFGPNAFPQRLGVSQLAAGSDGARLVCAEGADGTGKKTRTVSLICKPSALAKYLQKHCRTFSCYSPCGGWTWRASAFLQSAYEACWPCDSSVQFVRDNLQLSDDGLVSLDWAVPSHHRRRRTSSHSTSPVLLIIPNSFGKITRNVLCESALSHGYLPAVFNRRSHNSTPLTTLKLQQFGDPSDLREAVRYIRYRQPAGRLYAVSESSGSGLLLSYLGECGSSSYVTAVVCLSPVFRCQSWFESGLCWPLQWVLALYQKICLSYRTALGDIMHTDTLFSSCSLRGVEEALFCQSGYVGSTSGTSSGCSASGAWDAYWERNEPLRDVDEVAIPVLSVCAKDDPVRGDAQSSLPLELFETNPHFFLLLTDRGGHCGFSTQSNRSAGSAAGSVVATSTVDNHGPNWSHKVMLEFFRATTDFFNAEERAKQFAARRRGLGGAAGGWAFRYRSVSTCKRVPACSHNIHAIYNWQRSYTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYK8</accession>
    <name>A0A3B5LYK8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Interacts with PTBP2; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYK8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012879"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013036.1">
        <property type="protein sequence ID" value="ENSXCOP00000012879.1"/>
        <property type="gene ID" value="ENSXCOG00000009738.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155573"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22435">
        <property type="entry name" value="KH-I_NOVA_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22436">
        <property type="entry name" value="KH-I_NOVA_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09031">
        <property type="entry name" value="KH-I_NOVA_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000019">
        <property type="entry name" value="RNA-binding protein Nova-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000020">
        <property type="entry name" value="RNA-binding protein Nova-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000022">
        <property type="entry name" value="RNA-binding protein Nova-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047275">
        <property type="entry name" value="KH-I_NOVA_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047276">
        <property type="entry name" value="KH-I_NOVA_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047274">
        <property type="entry name" value="KH-I_NOVA_rpt3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="11">
        <location>
            <begin position="63"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="11">
        <location>
            <begin position="161"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="11">
        <location>
            <begin position="417"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="25"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="504" mass="51875" checksum="DC92E53F3E374F6D" modified="2018-12-05" version="1">MMAGGAVQQNGIFSSPHHHSQQPHMESDPPDSRKRPLETPTEASSTKRTNTGGKSRDGDAEEGEYFLKVLIPSYAAGSIIGKGGQTIVQLQKETGATIKLSKSKDFYPGTTERVCLIQGTVEALNGVHDFIAEKVREMPQSTQKTEPVSILQPQTTVNPDRVKQAKLIVPNSTAGLIIGKGGATVKAVMEQSGAWVQLSQKPEGINLQERVVTISGEPEQNRKAVEIIIQKIQEDPQSSSCLNISYSNITGPVANSNPTGSPYANSTEVMPAAAAAAAATASSLLGQAGLAGVGTFPTTMSSFSGNDLLAITSALNTLASYGYNTNSLGLGLNPAAASGVLAAVAANANPAAAAAANLLASYASDASTSVAHPVASLGGLSLGSLAAATGATNGYLSAASPLVASSLLATEKLAEGAKEVVEIAVPENLVGAILGKGGKTLVEYQELTGARIQISKKGEFIPGTRNRKVTITGSQAATQAAQYLISQRITYEQGVRATNPQKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZH7</accession>
    <name>A0A3B5LZH7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane channel-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TMC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZH7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016370"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016576.1">
        <property type="protein sequence ID" value="ENSXCOP00000016370.1"/>
        <property type="gene ID" value="ENSXCOG00000012367.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244894"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038900">
        <property type="entry name" value="TMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012496">
        <property type="entry name" value="TMC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302:SF5">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07810">
        <property type="entry name" value="TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="356"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="453"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="527"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="TMC" evidence="7">
        <location>
            <begin position="345"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU310713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="571" mass="65979" checksum="A2698F961F3236C9" modified="2018-12-05" version="1">ALSTTEQIRKIRELPVSFEEKKHDVYLCVLLCCLLLLQSMRRFGYSIKSARQTLQLWQGIMKEIGGKFGSSVLNYFVLLKWLLLFNIFSFLVNFGFITIPLLVYDLSLNVSPNVSFRGLELLTGAGYFNYTIMYYGSYSNETIRGLVDYDMQLAYFFTISVYMVLCGLVLIFSMASSFKKNYALADPASNSAWQLLCSWDFSITNEKAVNQRKNNLRIQLKESLSEKAHRERQTTSEQLKQYGIYFGSWLLSTGLAAGCGASIFYLYTETWSALKEAETLLVPFVVSLMNMVIPLFFSLFNKFEQYSSQRKQIYALLFRNVLLRISILGVLCYYWMNTVPQKFTCWEPMVGQALYRLVIVDFIFLMLGSLFGEFLSNVIGTKIQPRLGVPEFDVARNVLEIIYAQTLAWIGIYFSPLLPVIQILKFFILFYLKKVSLTHNCQPPRRSGRAAQMKTIFIFLLFFPFFVGALSIVAYTSWRLTPSEQCGPFRGLNSTFSVTGEWIDHVNNNSSNHWVGWIYQNVIRSEIFYFLSTLIILVIIYIFWQLIQGRKQLIVLLRRQIINVSLNCPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0I0</accession>
    <name>A0A3B5M0I0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0I0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016765"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016982.1">
        <property type="protein sequence ID" value="ENSXCOP00000016765.1"/>
        <property type="gene ID" value="ENSXCOG00000012644.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153680"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22265">
        <property type="entry name" value="UDM1_RNF168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051657">
        <property type="entry name" value="RNF168/RNF169_E3_ubiq-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23328">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23328:SF0">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="24"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="166"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="233"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="329"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="80"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="233"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="340"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="353" mass="39524" checksum="26698185A8444DBC" modified="2018-12-05" version="1">MSPVSEVEEGGRQELALTWEDCLCPVCLDIFIEPVTLPCTHSFCKLCFTNRIVCVRVCVCVCVRTVPRVFPRVCHPGELRQEYEDQVTKLMEERRAQDEEESKASEELIQKLLAEEQQQLQEEMRRKEEDERLAKLLSNQLVREVAERSRRGHRVLWVTLSCFPPTELPGEPSKSSELDPVEDEEVTSKRVQVCSPPGEERPLPDWEAELRVRKQQEEDDLHLALLLQRELDQEERRRATDRSKGSSDAYLLRAQGGDKQEAGPSHIPAGGRIRRMCSNSVSSSVSSSVSNSVSSSVSNSVSSSVSSSVSFPFLRSVSSASVVSRGSKQATLTEMFTLPRSRSSASAATRNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M1S2</accession>
    <name>A0A3B5M1S2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DEP domain-containing protein 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEPDC7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M1S2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015724.1">
        <property type="protein sequence ID" value="ENSXCOP00000015531.1"/>
        <property type="gene ID" value="ENSXCOG00000011742.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182976"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04446">
        <property type="entry name" value="DEP_DEPDC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04405">
        <property type="entry name" value="RhoGAP_BRCC3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16206">
        <property type="entry name" value="DEP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16206:SF9">
        <property type="entry name" value="DEP DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEP" evidence="3">
        <location>
            <begin position="27"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="514" mass="58284" checksum="B67D16EE984E2497" modified="2025-10-08" version="2">MFSPSEQSMAGKPFRATYIWNSIISNLQGQVEVKHRRHNLKSYHDCFLGSEAVDVVLSHITLTRFFGDEAVPRHKAVRLCQVLMDSKVFEPVGGKVFGKEKKRDKFEDSSVSLYRFLGPVTSSSLALTNTNSQSTGAIKSGYGSPNMNMSIGKKSIKKTKEKLLEEVLGNLNLSATIIPQMSHLGLSQECLYEVWHQQAVSRLLQLIELPLLESLLEGEVASRSSLHGMDSDPDLLCPTSYLDREVLKAFSEAQADEWMSVAVDCLEFLPDELVVEVSRGLAACADDLVQCKRLLYQVLVRHYGQTQRPPLLNNYIFDIHSGISELLVNGKLELALEALQLSLRLQDSHSQEELRRLLRFMATAAKPQEVKLHKEIENRMAVKRAFSRSIVYSGRLSKGKVDLMVLFMMDNYCDMFKIPISLHKMVSERLRNIVMGKDPDVITGTTYCSRVSAKEYSESAQKATQEEILSLLRTVYENPKFSRKEKRRLLGQFYKAHPETFVQYFGTKFYISKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M217</accession>
    <name>A0A3B5M217_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000018133"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018364.1">
        <property type="protein sequence ID" value="ENSXCOP00000018133.1"/>
        <property type="gene ID" value="ENSXCOG00000013668.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153612"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055444">
        <property type="entry name" value="ARM_ECM29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024372">
        <property type="entry name" value="Ecm29_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055443">
        <property type="entry name" value="HEAT_ECM29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346:SF19">
        <property type="entry name" value="PROTEASOME ADAPTER AND SCAFFOLD PROTEIN ECM29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR GCN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23702">
        <property type="entry name" value="ARM_ECM29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13001">
        <property type="entry name" value="ECM29_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24492">
        <property type="entry name" value="HEAT_ECM29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12755">
        <property type="entry name" value="Vac14_Fab1_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Proteasome component Ecm29 N-terminal" evidence="6">
        <location>
            <begin position="10"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="ECM29 ARM-like repeats" evidence="7">
        <location>
            <begin position="599"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome adapter and scaffold protein ECM29 HEAT-repeat" evidence="8">
        <location>
            <begin position="1249"/>
            <end position="1408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="187"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1555" mass="171205" checksum="CE9689D10B96E069" modified="2018-12-05" version="1">MAAQDELNQLERVFLRLGHAETDDQLQDIISKFLPPVLLKLSSVQEGVRKKVMELLVHLNKRIKSRPKIQLPVETLLVQYQDPAAASFVTNFTIIYIKMGYPRLEVAKQCELAPTLITAMEGKPQPQQDSLMHLLIPTLYHMKYPADSSKGVSPFNLIERPKMMHLLLEFMLDVLLMPYGFVLNESPTRPAPSSSQGGSSDGAVTGSGQGLPQPPPGMSVYAAKRVIGEAQWNAEQLEQCKLGIVKFIEAKQVPEVETVIHLAVASSDTRHSVATAADLELRSKQSIIDWNNPLIINKMFKVYLGDVPLKTKGGNVKQELKHDPVSTRVKLKILPHLLRSRLAAECFPANIQVVYDGLFGANTNNKLLSLTLQFVHHICMVCPNTNKPLGLMLLNGLTKLINEYKEVNKIPTVTCFFFYFSRMPQLFTKDIALVQQFFESMCKEEPDVRLAIQEALSMMVGAYANLQGALLNLMEALVAAYISKPEVQVRQVAMKFASTVFAPDHVPSRYLLLLAAGDPREEVSAEAQRVLRPFPTKSSEKEGPKPMPSFPDMVAYIQEKAAQRIKTPAKYIVGASTIPFNPSAFGEIMLYLRMCLAHSAGARPMSTRLLDMQDDAPAIGRYIHTLLSAEPPKGLEGNPVHVYMDLLQQLLSAVGGIPVMYCLLEVVSVCPEKLAPRFADKIDWIKSLMNTNKEDMRELAAQLYALVISTMTGNELQTAVQNLVKITKDNHSPETQHGAILALGYMVGRNLSRKKATASCDDRIYSHPGSFLDSGSAMLAVAACTALGEIGRNGTLLIPAEGDGFTKLSVTENLLARITSGKESVKMKERSITTLGYLPVGDSDFPHQKKLLQGLMDSVEAKQVELQFTVGEAITSAAIGTSSGAARDPWTCTEDQYSPPHNVKNNDVVRWVLNSILSRYIPSQNPHVRQAACIWLLSLVKKLSQHKEITSHLKEIQIAFISVLSDPDELSQDVASKGLGLVYEMGGEADQQELVSTLVETLMTGKRCFRGRIWEKRLMGDQSLSTYKELCSLASDLNQPDLVYKFMNLANHHAMWNSRKGAAFGFHMIATKAGEQLAPFLPQLVPRLYRYQFDPNLSIRQAMTSIWDALVTDKTLVDKYLKEILQDVISNLTNNTWRVRESSCLALNDLIRGRQADDLIDHLAEMWETLFRVLDDIKESVRKAADLALKTLSKVCVRMCESTGSAAQKTVAVMLPTLLEKGIVSNVSEVRSLSIQTLVKISKTAGARLKPHAARLIPALLEALSTLEPQVLNYLSLRATEQEKSAMDAARLSAAKSSPMMETVNMCLQHLDVSVLGELVPRLCELLKSGVGLGTKGGCASVIVSLTVQCPQDLTPYSGKLMSALLNGIHDRSTVVQKAFAFALGHLVRSAKDSSVEKLLLKLSNWYLEKEEPVYKSSCALTVHAISHYSPDVLKAHAGVALPLAFLGMHQAPGPDEEKGESHDATLWSEVWQENVPGSFGGIRLYMTELITITQKALQSQSWKMKAQGAAAMATVAKEQTGSLVAPHLGLVLTALMQGLSGRTWAGKVCVVPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2Q7</accession>
    <name>A0A3B5M2Q7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">DnaJ heat shock protein family (Hsp40) member B12a</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027862497.1">
        <property type="nucleotide sequence ID" value="XM_028006696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M2Q7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017600"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017823.1">
        <property type="protein sequence ID" value="ENSXCOP00000017600.1"/>
        <property type="gene ID" value="ENSXCOG00000013275.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114137862"/>
    <dbReference type="KEGG" id="xco:114137862"/>
    <dbReference type="CTD" id="324005"/>
    <dbReference type="GeneTree" id="ENSGT00940000155590"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000004">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily B, member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051100">
        <property type="entry name" value="DnaJ_subfamily_B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015399">
        <property type="entry name" value="DUF1977_DnaJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908:SF8">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09320">
        <property type="entry name" value="DUF1977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="9">
        <location>
            <begin position="109"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="43"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="369" mass="41975" checksum="41A20FFA8E258930" modified="2018-12-05" version="1">MDSNKDEAERCIKIALNAIGNNQPDKARKFLEKAQRLFPTDKAKNLLESLAQNGKPPDENGSAVNGGGPGLRHRGERDGANASQGPSESDKSYTAEQLEAVRKVKSCKDYYQILGVEKAASEEDLKKAYRKLALKFHPDKNHAPGATEAFKAIGNAYAILSNAEKRRQYDQYGEERTQPSRHRHHHDFEADISPEDLFNMFFGGGFPTSNVHVYRNGRMHFAHQNRQERREQQRDGGFALLVQLMPILILIVVSALSQLMVTQPPYSLSYRPSAGHIHKRHTSHLKVPFFVGDRFNEEFTGNNLKNVERNVEEDYISNLRNNCWKEKQQKEGLLYRARYFGDSELYQRAQRMGTPSCSRLSEIQVILDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3X0</accession>
    <name>A0A3B5M3X0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3X0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019089.1">
        <property type="protein sequence ID" value="ENSXCOP00000018853.1"/>
        <property type="gene ID" value="ENSXCOG00000014190.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163346"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11461">
        <property type="entry name" value="bHLH-O_HES5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="19"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="8">
        <location>
            <begin position="91"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="137"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="18630" checksum="266A6357BD7A6453" modified="2018-12-05" version="1">MAPAITAAMTSSQEHLMLNHKLRKPQVEKLRRERINSSIEQLKSLLLGAEFLKQQPDSKLEKADILEMTVCFLRQLQKQHPDVDSGAVELGYSRCVQETVHFLSKDEVKTQSQRKLLKHFSTMQSFSMKILRKADFSPQSSTEQGSVSKDKNPLHSPLWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3Y1</accession>
    <name>A0A3B5M3Y1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Angiotensin II receptor-associated protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000018863"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019099.1">
        <property type="protein sequence ID" value="ENSXCOP00000018863.1"/>
        <property type="gene ID" value="ENSXCOG00000014198.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017402"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038166">
        <property type="term" value="P:angiotensin-activated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008217">
        <property type="term" value="P:regulation of blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009436">
        <property type="entry name" value="AGTRAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16521">
        <property type="entry name" value="TYPE-1 ANGIOTENSIN II RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16521:SF3">
        <property type="entry name" value="TYPE-1 ANGIOTENSIN II RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06396">
        <property type="entry name" value="AGTRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00805">
        <property type="entry name" value="AGTRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="136"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="163" mass="18213" checksum="0F26ECD4C711A880" modified="2025-10-08" version="2">MXXXSFLFQAIVLAHWLLTVWGSMSYLPSSYAWGNFSVLAVGVWAIAQRDSIDAVLMFLMGMAVTILTDIVHFGIIYPQYPLVAAASFSAGMAILNLLLKPVSCFFVYQMYRERGGDYNINFGFPSVSRNRDAYQSIDQQDESSSPTNSFNQAKDTKPGVRTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M430</accession>
    <name>A0A3B5M430_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">ATP-binding cassette, sub-family C (CFTR/MRP), member 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>17beta-estradiol 17-O-(beta-D-glucuronate)(in) + ATP + H2O = 17beta-estradiol 17-O-(beta-D-glucuronate)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60128"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:82961"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:60129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O + xenobioticSide 1 = ADP + phosphate + xenobioticSide 2.</text>
            <dbReference type="EC" id="7.6.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>leukotriene C4(in) + ATP + H2O = leukotriene C4(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38963"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57973"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:38964"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000016181"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016387.1">
        <property type="protein sequence ID" value="ENSXCOP00000016181.1"/>
        <property type="gene ID" value="ENSXCOG00000012227.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164383"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18595">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000001">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000007">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000997">
        <property type="entry name" value="Multidrug resistance-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000074">
        <property type="entry name" value="Multidrug resistance-associated protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005292">
        <property type="entry name" value="MRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00957">
        <property type="entry name" value="MRP_assoc_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF176">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="394"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="470"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="956"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1029"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1137"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="19">
        <location>
            <begin position="256"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="18">
        <location>
            <begin position="568"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="19">
        <location>
            <begin position="910"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="18">
        <location>
            <begin position="1230"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="808"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="854"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="854"/>
            <end position="867"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="22">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1475" mass="166118" checksum="00BD5250D23E9D34" modified="2018-12-05" version="1">MCAAFLEEYCGSVFWVGLTILLLLCQEGVRRKEKAVDSSSLFLFWLLLVLCDIFPFQTLIREALRLVRDVPRFSLFYISFGLTLIALILSALADMSPETKELVKKNPGAGATFLSRITFNWFNGMVMKGYKKPLVQEDMWELNEADSTSYISQRFNHHMQFELGKARIRFQKELKDKLLKNRSKSMEAPSQSNGLGKGVSQDVLMMVRTFKYTKQPVQEEKGKKDDENKKKEVTNYPNSWLVTTIYKTFKWLLIESAFFKLLQDLLAFVSPQLLKLMISFTQDKSSYAWEGYLYAVLLLVVALLQSLFLQQYLQRCFVLGMKVRTALMAAVYKKALVMSNDNRKESTVGETVNLMSADAQRFNDVTNFIHLLWSCPLQIMVSIVFLWMDLGPSTLAGVAVMLLMVPVNGLLANQAKKIQRENMKSKDKRLKIMNEILNGIKTLKLFAWEPSLQTQVEDIRGEELKVMRKFAYLTSFSTFISNCAPTLVSLATFAVFVAVSSDNILTAEKAFTSISLFNILRQPLSMLPMLIASIVQTTVSKKRLEKFLGGDDLENDIVCHDPSFNFAVSICDGSFAWEKDSEPLLKSVSLGIKPGGLVAVVGAVGSGKSSLLSALLGEMHRTKGFINIQGSFAFIPQQAWIQNATLRDNILFGSPYEEQRFEDVLEACALGPDLKLLSAGDLTEIGEKGINLSGGQKQRVSLARAAYSQADIFLLDDPLSAVDSHVGKINVLDKSMGKTLPNNAELSFFYYFVSLQTRVLVTHGVSFLPCVDEVVILVNGRVSEVGSYQSLRASKGAFSEFLDTYGTEQSNQTSSEDGCHNKEDFISDSEDNQSDVPLADAVSLMLRRENSIRRSQRGNRSRLRKNSNLKSPENTKKGQRLIEKETMETGQVKFSVWFQYVQAMGWGYAFWALFIYIIQNVAFIGQNLWLSDWTNDAVEHYNQTYSPMKRNTRVGVFGALGMTQGLFVFLGTLLLTNAAVNASRVLHSKLLNNVLRVPMVFFDTTPLGRVLNRFAKDIFTIDEVIPQMFSSWIMYLLGILGTIVFLCLSTPLFIIVIIPLAAIYFFVQRFYIPTSRQLRRLESVSRSPIYSHFGETMSGLSVIRAYNHQERFMKHNEVTIDENLKTIFLRIMSNRWLAIRLQFLGNLVVFFAAVFAVISRNSIDSGLVGLSISYALNVTLTLNFVVSITSQLETNIVAVERVSEYSELENEAPWVTQHRPSEKWPEVGSLQFVDYKVRYRPELDLVLKGISCDISGTEKIGIVGRTGAGKSSLANCLFRIIEAAEGRILIDNLDISTIGLHDLRNRLTIIPQDPVLFSGTLRMNLDPFDKFSNEELWRVLELSHLKDYVAGLKEGLKHEVAEGGENLSVGQRQLLCLARALLRKSQILILDEATAAVDLETDDRIQNTIRKEFSHCTVLTIAHRLHSIMDSSRVMVLDAGKIVEFDSPSNLMEKRGYFYSMAKDAGITHESETTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M595</accession>
    <name>A0A3B5M595_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Anti-silencing function 1Bb histone chaperone</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ASF1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M595"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019333"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019571.1">
        <property type="protein sequence ID" value="ENSXCOP00000019333.1"/>
        <property type="gene ID" value="ENSXCOG00000014537.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004692"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1490:FF:000001">
        <property type="entry name" value="Histone chaperone ASF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1490">
        <property type="entry name" value="Histone chaperone ASF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006818">
        <property type="entry name" value="ASF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036747">
        <property type="entry name" value="ASF1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040">
        <property type="entry name" value="ANTI-SILENCING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040:SF22">
        <property type="entry name" value="HISTONE CHAPERONE ASF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04729">
        <property type="entry name" value="ASF1_hist_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101546">
        <property type="entry name" value="ASF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="199" mass="22189" checksum="7E2568FC300B473E" modified="2018-12-05" version="1">MAKVQVLNVAVLDNPSPFGNPFQFEITFECMEDLPEDLEWKIIYVGSAESEEYDQVLDSVLVGPVPAGRHMFVFQADAPNTGLIPETDAVGVTVVLITCTYRGQEFIRIGYYVNNEYTDPELRENPPLKPDYSQLQRNILASNPRVTRFHINWEGSADKMEDSENVDPSPNVSGMLPPSCIPGKMPALGQMPDNSMDCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5B8</accession>
    <name>A0A3B5M5B8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M5B8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015174"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015364.1">
        <property type="protein sequence ID" value="ENSXCOP00000015174.1"/>
        <property type="gene ID" value="ENSXCOG00000011497.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158905"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019871">
        <property type="term" value="F:sodium channel inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007528">
        <property type="term" value="P:neuromuscular junction development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032801">
        <property type="term" value="P:receptor catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048814">
        <property type="term" value="P:regulation of dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF282">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEDD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="8">
        <location>
            <begin position="15"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="106" mass="12207" checksum="0B61DC351E83C241" modified="2018-12-05" version="1">MSKEMFNPYYGLFEYSTVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTSDKLPRAHTCFNRLDLPPYESFEELREKLNIAIENAQGFDGVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6M0</accession>
    <name>A0A3B5M6M0_9TELE</name>
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        <submittedName>
            <fullName evidence="7">Transmembrane BAX inhibitor motif containing 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6M0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019364.1">
        <property type="protein sequence ID" value="ENSXCOP00000019127.1"/>
        <property type="gene ID" value="ENSXCOG00000014374.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244890"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001234">
        <property type="term" value="P:negative regulation of apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10428">
        <property type="entry name" value="LFG_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF35">
        <property type="entry name" value="PROTEIN LIFEGUARD 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="283" mass="31507" checksum="C60B19B80A37EA8D" modified="2018-12-05" version="1">MSSKTDSPPSYEDTLKDPKPRLPSGCHAHPHSVSRTVCVQPRFPGLKLPFFAILNSAGEMDDFFSTQWESTSIRHAFIRKVYLILTVQLAFTFAVVGVFTFQNHIPDLFLHSAVFFLVYCILICCKEPRRRFPLNLVLLGIFTVALSYMAGTVSSYYETKAVIIAMGITGVVCIAVTVFCFQTKVDFTSCGGFLCIAAVLLMIIGIVTSIVLSFQYVPWLHMLYAAIGAIVYTLFLVYNTQLLIGNRELAISPEEYIYGALSLYIDIVQIFLFILQVSGAATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7L9</accession>
    <name>A0A3B5M7L9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7L9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017321"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017542.1">
        <property type="protein sequence ID" value="ENSXCOP00000017321.1"/>
        <property type="gene ID" value="ENSXCOG00000013053.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155224"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1082">
        <property type="entry name" value="COLLAGEN TRIPLE HELIX REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017207465" evidence="4">
        <location>
            <begin position="27"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="105"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="172"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="185"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="290"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="302" mass="29724" checksum="A3D15E4648536311" modified="2018-12-05" version="1">MGPTGAAVRGLYFAPFLFLTTLPGQFRTILQRRVGQNSSTTSKKRNKVRKGQILPHTAVVVVTHLSNKSLHLFIFSASNTSILKRKFMLFTPLINPINPLHPIIQGDQGAKGADGAPGKDGPRGLTGPIGLPGPAGAPGDKGENGAPGLVGPAGARGAPVSKICLSPKNLSQLGERGEAGPPGPAGFAGPPGADGQPGAKGESGDNGAKGDAGPPGAAGPTGAPGPQGNSGPPGPPGPSGKEGPKGNRGETGPAGRPGEIGASGPPGNPGEKGSPGADGAAVSKLLCNRLSKQSRSPSGENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7X2</accession>
    <name>A0A3B5M7X2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Diacylglycerol kinase</fullName>
            <shortName evidence="11">DAG kinase</shortName>
            <ecNumber evidence="11">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + ATP = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40327"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:40328"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; glycerolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7X2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019835.1">
        <property type="protein sequence ID" value="ENSXCOP00000019590.1"/>
        <property type="gene ID" value="ENSXCOG00000014663.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159492"/>
    <dbReference type="UniPathway" id="UPA00230"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.40:FF:000004">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000012">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF54">
        <property type="entry name" value="DIACYLGLYCEROL KINASE THETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DAGKc" evidence="13">
        <location>
            <begin position="79"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="388"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="417" mass="45723" checksum="D325AD2836EE096A" modified="2018-12-05" version="1">MSQHLVTMTTAIYRMVWFTRCCNCRQTFAAFYSCCCFQTCLYFLRLLQFSVAVCFCPSASVALTHFPPCRSLQCQKLGDAVCPLLVFVNPKSGGLKGRELLHSFRKLLNPHQVFDITNGGPLAGLHAFREVPRFRVLVCGGDGTVGWVLGVLETVRHKLVCREPPIGIVPLGTGNDLARILRWGPGYGGDDPHHILASVDEADEVQMDRWTILLDMNNYFGLGIDAELSLDFHLAREGEPDKFTSRFHNKGVYVKVGLQKISRSRSLHKELQLQVDKQPVPLPNIEGLIFINIPSWGSGADLWGSEADGRFSRPRIDDGLLEVVGVTGVVHMGQVQSGLRCGIRIAQGSYIRLIVSKELPVQVDGEPWLQAPGHIIISAAGPKVLRIGPAGSRSSTQARLDPARPAGSLVTGSIHPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7Z3</accession>
    <name>A0A3B5M7Z3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GRB10 interacting GYF protein 1a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7Z3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015899"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016096.1">
        <property type="protein sequence ID" value="ENSXCOP00000015899.1"/>
        <property type="gene ID" value="ENSXCOG00000012010.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159845"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048009">
        <property type="term" value="P:insulin-like growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051640">
        <property type="entry name" value="GRB10-interact_GYF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445">
        <property type="entry name" value="GRB10 INTERACTING GYF PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14445:SF37">
        <property type="entry name" value="GRB10-INTERACTING GYF PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="11889" checksum="06D4468497A808AD" modified="2018-12-05" version="1">MTAETLNFGPEWLRALSSGGSVTSPPPSPAMPKYKLAEYRYGREEMLALYIKDNKVPEDMQDKEFAAILQDEPMQPLALVPLTEEEGRQRATMCGNCFQNTNYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8V8</accession>
    <name>A0A3B5M8V8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019955"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020202.1">
        <property type="protein sequence ID" value="ENSXCOP00000019955.1"/>
        <property type="gene ID" value="ENSXCOG00000014983.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158137"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd13322">
        <property type="entry name" value="PH_PHLPP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17240">
        <property type="entry name" value="RA_PHLPP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000173">
        <property type="entry name" value="PH domain and leucine rich repeat protein phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000027">
        <property type="entry name" value="PH domain and leucine rich repeat protein phosphatase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000003">
        <property type="entry name" value="PH domain and leucine-rich repeat protein phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055071">
        <property type="entry name" value="RA_PHLPP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF43">
        <property type="entry name" value="PH DOMAIN AND LEUCINE RICH REPEAT PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23010">
        <property type="entry name" value="RA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="12" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
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    <sequence length="1373" mass="151525" checksum="BBAFBD50D613B6E9" modified="2018-12-05" version="1">MPATSASNRAVASNGRGSSSNSSSLLLRRRRLKRNLSAAAAATTTAASSAVTAKMSSALSSASLHTRSLDRKTLLKHRLNQQLQPADREWVRADLHRGCIHVHDRLTSSYPRPVLCTMDNTAGEVADTSPTLYVQLHGEAVRRLSPDERPLQIQNDFLFKLGFKDPWRVQEEGLNTEIGSLLRFYAGKFFHIVFLLRVQLSGTYNVRKGKLQLPVNRWTRRQVILCGTCLIVSSVKESQIGKMHILPLIGGKVEEVKKHNHCLAFSSAGPQSQTYYVSFDSFTEHLRWHRHAAKMVSQRINSVDLSCCSLELLPPNLFYSHDLTHLNLKHNFLPADHRLQFSRLRSLNLSNNHLGQFPLAICDIPTLTEVNLSCNYMDSVPSSVGAMSNLQTFLLDGNSLSELPCELGSLQRLSYLGLSFNQFSHVPQVLERLASMEKLCMAGNNQETLTLQNFRLLHVKHIDLRLNKISVMVPDEPDLLRHVTQLDLRDNQLTELDASIFPRLEVLHCERNHITCLKVKGSLLKGIYASNNELRQLDVSPVPSNLTYMDISRNHMESLPDWLCEAKKLEVLDVSHNLITELPARLFCSNSLRKLSAGHNQLQKLPQRVERPLLEVLDVQHNQLVELPCNLFLKSNSLRCVNASANKLEHLPPSSLSEESHSILQELYLTNNWLTDKCVPMLTGHTHLRVLHMAYNHLQTFPASKMAKLEELEEADLSGNMLKTVPTTIMNCRRMHTLIAHSNSIEVFPEVMQLMEMKCVDLSCNELSEITLPENLPPKLQELDLTGNPRLNLDHKTLEHNIRCFRIDPPPTFSSNEASGGPAVWSHGYTEASGVKNKLCVAALSVNSFCGSREALYGVFDGDRNVEVPYLLQCTMNDVLAEEIHKTKSEEDYMTNTFLVMQRKLGTAGQKLGGSAALCHIRHDPTDPSGCFILTAANVGKCQAILCRDGKPMSLSLLHNVGLEEEYRRIRQHRAIVTEDNKVNGVTDSTRIMGYSFLYPSVIPCPYVQTVTLTPQDEFFILGSRGLWDAVSPTEAVEAVRNVPDGLAAAKKLCTLAQGYGCTDSLSAVVVQLSVSEDCCSCCCSDPPQPPPSPGLGAYPSSSSAIKDRPSDGSLPVPPSSCSEISSEISTSEMSSEVGSTASSDEPPQKLASARNCCALHPACLAGSFQRQLSSATFSSALSDNGLDSEDEEPIAGVFSNGSRVEVEADVHCLKAELSQLSSPQSLLPVTSSQERTLLNLPPPPPPPCTPEPLEDSIDLNLAEAENGLERDESWPGVGAGAGDGGKFPGKRRVNGSVARQEKSHNLIEVAADAPSKKSGGYFTAPAQPDPDDQFIIPPELEEEVKEIMKQHQQKQQKPAGTDQPTDYYDTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9A0</accession>
    <name>A0A3B5M9A0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Anion exchange protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>hydrogencarbonate(in) + chloride(out) = hydrogencarbonate(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:72363"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9A0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016545.1">
        <property type="protein sequence ID" value="ENSXCOP00000016339.1"/>
        <property type="gene ID" value="ENSXCOG00000012326.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158259"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008509">
        <property type="term" value="F:monoatomic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015701">
        <property type="term" value="P:bicarbonate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000001">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.930.10:FF:000004">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="entry name" value="Mannitol-specific EII, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001717">
        <property type="entry name" value="Anion_exchange"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013769">
        <property type="entry name" value="Band3_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00834">
        <property type="entry name" value="ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF14">
        <property type="entry name" value="ANION EXCHANGE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07565">
        <property type="entry name" value="Band_3_cyto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00165">
        <property type="entry name" value="ANIONEXCHNGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01231">
        <property type="entry name" value="HCO3TRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="Phoshotransferase/anion transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0039">Anion exchange</keyword>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="670"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="712"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="755"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="787"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="853"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="886"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="943"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="983"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1013"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1042"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1065"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1115"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1139"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="domain" description="Band 3 cytoplasmic" evidence="15">
        <location>
            <begin position="278"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="14">
        <location>
            <begin position="641"/>
            <end position="1171"/>
        </location>
    </feature>
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            <begin position="1"/>
            <end position="38"/>
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    </feature>
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            <begin position="1"/>
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            <begin position="89"/>
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    </feature>
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            <begin position="115"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00010993"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00955"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF07565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
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            <dbReference type="Ensembl" id="ENSXCOP00000016339.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1193" mass="134668" checksum="920D889DD4FD3B0C" modified="2018-12-05" version="1">SKNPLTEDTLYNSLMNTSSLHGHQQRGEEEDEGDLNKALGVQRFQHILSPAASVPDELLHSYHEEDIEYHRHYSRHIHRPLSKLPTEGRRKKSGKKRKKDKDNKSSRAPSSGPIEEGEDEEEEEEESIEANSVLSESEKPRDVEVLKISQISTFSPPSCSKPDSPSSPSPTSRSASPENPPLPRVSSASRSYDLQDRRRTGNMTGAEQAKYQRIPTDESEALTLASADLDGIKSHRFEDVPGVRRHLVRKSTKGQMVHIGKDHKEPTTRSKKQDRTPHEVFVELNELTMDKNQEMQWKETARWIKFEEDVEEETDRWGKPHVASLSFRSLLELRRTISHGAVLLDLDQKTLPGIAHQVVEQMIISDQIKAEDRSNVLRALLLKHSHPSDEKEHVSHFPRNISAASLGSLLPYHHTANHNHQLEPSVTDPLMGTVNSTVDNDARIDIDRNEPQKEPCLGPGMHRSKSKHELKLLEKIPENAEATVVLVGCVDFLEQPTMAFVRLQEAVELESVLEVPVPVRFLFVLLGPPTTSMDYHQIGRSISTLMSDKQFHEAAYLADDRQDLLNAINNFLDCSIVLPPSEMGDDELLRSVARFQREMLRKREEQEQVKLLAKEPKSFEEKEALLKPSKQSDDPLKRTNRPFGGLIRDIQRRYPKYISDFSDALNSQCMAAVIFIYFAALSPAITFGGLLGEKTDGLIGVSELIISTAVQGILFCLLGAQPLLVVGFSGPILVFEEAFYSFCKANSMEYLTGRVWIGFWLIVIVIFTVAFEGSFLVRFVSRFTQEIFSFLISLIFICETFIKLGRIFKEHPLKRCSINSTTEEEEKVVEGNIMMREHNDTHIFTVTGEPNTALLSLVLMAGTFFIAFYLRKFKNSAFFPGSCRRIIGDFGVPIAILIMVLMDYSIEDTYTQKLSVPSGFSVTSPNKRGWIINPLGSDGQFPVWMMFACCLPALLVFILIFMETQITTLIVSKKERMLLKGSGFHLDLLLIVVLGGSSALFGLPWMAAATVRSVTHVNALTVMSTAVAPGDKPRIQEVKEQRVTGLLVAIMVGLSIVIGDLLRQIPLAVLFGIFLYMGVMSLNGIQLTERMMLLLMPSKYHPDHTYVRKVRTWRMHLFTCIQMVCLAVLWAVMSTQASLAFPFVLLLTVPVKMFVLRRIFNEREIACLDGDDAEPKFDERDCYDEYSEMHMPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAP2</accession>
    <name>A0A3B5MAP2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027861774.1">
        <property type="nucleotide sequence ID" value="XM_028005973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAP2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018341"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018573.1">
        <property type="protein sequence ID" value="ENSXCOP00000018341.1"/>
        <property type="gene ID" value="ENSXCOG00000013812.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114137415"/>
    <dbReference type="KEGG" id="xco:114137415"/>
    <dbReference type="CTD" id="556881"/>
    <dbReference type="GeneTree" id="ENSGT00390000004284"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031718">
        <property type="term" value="F:type 1 cannabinoid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029204">
        <property type="entry name" value="CNRIP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31952">
        <property type="entry name" value="CB1 CANNABINOID RECEPTOR-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31952:SF3">
        <property type="entry name" value="CB1 CANNABINOID RECEPTOR-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15043">
        <property type="entry name" value="CNRIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="162" mass="18243" checksum="E92276AEC5461AFD" modified="2018-12-05" version="1">MADVPQVVKIGISLKMLPNNSAVFFKSDGARFGQTRTIKLLTGSKYKIEVVVKPGAVQATSMSVGGVTFPLEQQSKDPQSAVYTGQYDTEGVAHTKSGERQPVQINIQFTEAGSFETVWQVKFYNYNKRDHCQWGNSFNSIEYECKPNDTRTLMWINKEMFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAQ2</accession>
    <name>A0A3B5MAQ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bZIP family. NFIL3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAQ2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020662"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020913.1">
        <property type="protein sequence ID" value="ENSXCOP00000020662.1"/>
        <property type="gene ID" value="ENSXCOG00000015482.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164623"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14694">
        <property type="entry name" value="bZIP_NFIL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000025">
        <property type="entry name" value="nuclear factor interleukin-3-regulated protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047106">
        <property type="entry name" value="NFIL3-like_bZIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07716">
        <property type="entry name" value="bZIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="59"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="131"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="403"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="474"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="474"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="532"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="561"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="577" mass="63934" checksum="4F92697CE51C5DEA" modified="2018-12-05" version="1">MENLTLNKPSGNNLNSIETFDSYDETLQGTPARLGRLIKPKPNVSCRRKRQFISDEKKDASYWEKRRKNNEAAKRSREKRRLNDMVLENRVIALNDENVRLKTELLQLKLRFGLISTASYIEKSQQISVANDGGNGGSSSSSSSNQYYSSGYSSGSQVMINSDSSETEQSGHSDSHRQLVKYSPRGSLSDMSDGSSRDSPEPMPFEIKQEGDRLEMDITSDTTTQIMFNIHRGPASGSTSHQIKQHQEMDAAYHHHPHLLQQQSQHHQVTVAAVKTTSQTVPHPPAAQRSVILYGASSASYPVDVMTRPQDVDLQVALKHSSSQLQQPITKTSIETLAEVTKQLERKTLDSPQYELSDSFNEAKEREVYRVCQPTQQEGGSPKEILSEQVEGVRHTQLYHHLQQPHHSYRSAQDEEPPVLSYEGGPRADGYLQSQSTLLSDGDPSDKEASTDDEESLSLSCSDMSSIHNQHLSGILQSGSPLPQYPGCSQMQGWDPQGEVRGTALPHKLRLKHRAMSTGSSGGNCSGQESPTTPPSATPPPLPQHPYLSLSPLQNIKRESSSGNQQIISYSSTCDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MD14</accession>
    <name>A0A3B5MD14_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Synaptophysin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homohexamer or homotetramer. Interacts with SRCIN1. Interacts with VAMP2; the interaction is inhibited by interaction of VAPM2 with SEPT8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Synaptic vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Synapse</location>
            <location evidence="15">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptophysin/synaptobrevin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027867385.1">
        <property type="nucleotide sequence ID" value="XM_028011584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MD14"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022153"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022422.1">
        <property type="protein sequence ID" value="ENSXCOP00000022153.1"/>
        <property type="gene ID" value="ENSXCOG00000016544.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114141135"/>
    <dbReference type="GeneTree" id="ENSGT01030000234637"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048786">
        <property type="term" value="C:presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001285">
        <property type="entry name" value="Synaptophysin/porin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10306">
        <property type="entry name" value="SYNAPTOPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10306:SF10">
        <property type="entry name" value="SYNAPTOPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00220">
        <property type="entry name" value="SYNAPTOPHYSN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="14" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0770">Synapse</keyword>
    <keyword evidence="5" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="MARVEL" evidence="20">
        <location>
            <begin position="15"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="246"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="253"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="278"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00062829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="292" mass="32689" checksum="267D6D735816C316" modified="2018-12-05" version="1">MDVVNQLVAQGQFRILKVPLGFIKVLQWFFAIFAFSTCGSYSGMFKMAVECRNRTESDLAIQVEFEYPFRLHQVYFDAPTCKDRATDRIFLVGDYSSSAEFFVTIGVFAFLYSTAALSIYVFFYDKYKENNKGPLIDLGVTAVFAFMWLVSSAAWAKGLSDVKTATDPDEVITLIDACEKKENSCREVHDPVMSGLNTSVAFGFINLVLWAGNLWFVFKETGIIAPFMRAPPPEGKPAPDAYAQQGAYEQDPYASNQGGYQPEFNQQGYNQEAEYGQGYSQQGAPTSFSNQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDL3</accession>
    <name>A0A3B5MDL3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">COP9 signalosome complex subunit 8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CSN8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDL3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021615"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021879.1">
        <property type="protein sequence ID" value="ENSXCOP00000021615.1"/>
        <property type="gene ID" value="ENSXCOG00000016159.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166277"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010387">
        <property type="term" value="P:COP9 signalosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.990:FF:000011">
        <property type="entry name" value="COP9 signalosome complex subunit 8-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033205">
        <property type="entry name" value="COP9_CSN8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033464">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13339">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13339:SF0">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10075">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0736">Signalosome</keyword>
    <feature type="domain" description="PCI" evidence="8">
        <location>
            <begin position="23"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="214" mass="24304" checksum="D990EEB3869A0435" modified="2018-12-05" version="1">MDQNSSKCFSFRRFLSQSLFPKPFFESVCLYLKENDIQNNHLHYLPQAPGGIATPQVYAQMLALYLLNNDMNNARYLWKRIPQAIKSANPELAAIWAVGQRIWQRDFPGIYTTIAAHQWSENILPVMEALRESSRQRAYSLVAQAYTSITAEDFAAFVGYSVEEAVKGVVSQGWQADPATRMVMPKKPDPPPVSLVPNEQQLARLTDYVAFLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEH2</accession>
    <name>A0A3B5MEH2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EAF7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEH2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021950"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022217.1">
        <property type="protein sequence ID" value="ENSXCOP00000021950.1"/>
        <property type="gene ID" value="ENSXCOG00000016403.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007823"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012423">
        <property type="entry name" value="Eaf7/MRGBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13581">
        <property type="entry name" value="MRG-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13581:SF5">
        <property type="entry name" value="MRG_MORF4L-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07904">
        <property type="entry name" value="Eaf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="158"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="164"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="177"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="208" mass="22680" checksum="2E67023F54AF6C40" modified="2018-12-05" version="1">MGEADVTLSQTEEKPPDSALVPGEDSVVWSHEVEVCLFHAMIGHKPVGVNRHFHMICIRDKFSQNIGRQVSSSVIWDHLGTMYDMQALHESEILPFPNVEKSFSLPEEIIQEVKGGKLGSEEEIKEEFRMEREPPATLEEGQALLLLFSATCVLSGRDKEKGGGEGGAAAAVGGGAKEAEKRKRSRATEKLLSSSNPASPGGAKRRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFI3</accession>
    <name>A0A3B5MFI3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Si:ch211-246m6.4</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MFI3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022472"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022747.1">
        <property type="protein sequence ID" value="ENSXCOP00000022472.1"/>
        <property type="gene ID" value="ENSXCOG00000016793.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166136"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11465">
        <property type="entry name" value="bHLH_TS_scleraxis_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000010">
        <property type="entry name" value="Scleraxis bHLH transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050283">
        <property type="entry name" value="E-box_TF_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, TWIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23349:SF40">
        <property type="entry name" value="BHLH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="58"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="180" mass="20030" checksum="A9CDFDFB1DA8731D" modified="2018-12-05" version="1">MRQIFISVPKDGTGADNGGHCRVRPGPTWTVHSAGAQSRRPKFASGEEFKTTGPQKGSVYHRTRRLCRDRTHSVNTAFTALRTLIPTEPADRKLSKIETLRLASSYISHLANVLLLGEDCRDEQPCLRYQDLILHGPAPLSGPSLRPICTFCLSNQRKQVGPFLDRLPLIKISNRTVTNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MG27</accession>
    <name>A0A3B5MG27_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MG27"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023273"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023551.1">
        <property type="protein sequence ID" value="ENSXCOP00000023273.1"/>
        <property type="gene ID" value="ENSXCOG00000017379.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047225"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032281">
        <property type="term" value="C:AMPA glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046464">
        <property type="term" value="P:acylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050266">
        <property type="entry name" value="AB_hydrolase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798:SF32">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING 6, ACYLGLYCEROL LIPASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43798">
        <property type="entry name" value="MONOACYLGLYCEROL LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="73"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="239" mass="26848" checksum="BFDA22061B850D00" modified="2018-12-05" version="1">MELRWAKKLLLTGGVLLVPVFALVTSVVFWPGVLLKAYSWYMCLSQGVAVRYSHSASYRFCYSTRGTPGCATPTLLLLHGFSTSKEMWLSVIKYLPRNLHVVCLDMPGHEGTSRTGAEDYSIQGQARRVHQVRANFLRGLLANRIPNNNFYREVFLAIIGEKSRHSLQQHLNLITAPLQVIWGKNDQILDVSGAAVIQKVLPHCQVDLLDNCGHAIALERPRKFANLVLDFHNKAKKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGH0</accession>
    <name>A0A3B5MGH0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">E3 ubiquitin-protein ligase MARCHF5</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Membrane-associated RING finger protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Membrane-associated RING-CH protein V</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">RING-type E3 ubiquitin transferase MARCHF5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027881040.1">
        <property type="nucleotide sequence ID" value="XM_028025239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGH0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022837"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023113.1">
        <property type="protein sequence ID" value="ENSXCOP00000022837.1"/>
        <property type="gene ID" value="ENSXCOG00000017065.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114149403"/>
    <dbReference type="KEGG" id="xco:114149403"/>
    <dbReference type="CTD" id="326067"/>
    <dbReference type="GeneTree" id="ENSGT00390000009948"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16701">
        <property type="entry name" value="RING_CH-C4HC3_MARCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000262">
        <property type="entry name" value="E3 ubiquitin-protein ligase MARCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46283">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="21">
        <location>
            <begin position="4"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00043044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00043185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="288" mass="32217" checksum="4FB527FA9AECD422" modified="2018-12-05" version="1">MAQTEEQSEKHCWVCFATERDDRGAEWVSPCRCKGCTKWIHQSCLQRWLDEKQKGNGGAAVNCPQCGTEYCIIFPKMGPLVYFLQQADRALSRASPFAAVGVVVGTVYWSAVTYGAVTVMQVVGHKKGLFVMERADPLFLLMGLPTIPVLLVLGKMIRWEDYLVRLWQRYSYKHGPSSGGGSYLPRLRAEGPGAGDHLSVSRTLCGALIFPSIASLVGWLLFRRVTSNLQRTILGGIAFVLIKGILKVYFKQQQYIMQANRHILNYPESHKNRLGGEGEDNTEDGRNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKM8</accession>
    <name>A0A3B5MKM8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">5-hydroxytryptamine receptor 3A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">5-hydroxytryptamine receptor 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Serotonin receptor 3A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Serotonin-gated ion channel receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Forms serotonin (5-hydroxytryptamine/5-HT3)-activated cation-selective channel complexes, which when activated cause fast, depolarizing responses in neurons.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Mg(2+)(in) = Mg(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29827"/>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="22">Forms homopentameric as well as heteropentameric serotonin-activated cation-selective channel complexes with HTR3B or HTR3C or HTR3D or HTR3E. The homomeric complex is functional but exhibits low conductance with modified voltage dependence, and decreased agonist and antagonist affinity. Heteropentameric complexes display properties which resemble that of neuronal serotonin-activated channels in vivo. Interacts with RIC3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Postsynaptic cell membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKM8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024929"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025233.1">
        <property type="protein sequence ID" value="ENSXCOP00000024929.1"/>
        <property type="gene ID" value="ENSXCOG00000018613.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157705"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19011">
        <property type="entry name" value="LGIC_ECD_5-HT3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19063">
        <property type="entry name" value="LGIC_TM_5-HT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000020">
        <property type="entry name" value="5-hydroxytryptamine (serotonin) receptor 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000017">
        <property type="entry name" value="5-hydroxytryptamine receptor 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008132">
        <property type="entry name" value="5HT3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008133">
        <property type="entry name" value="5HT3_rcpt_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049944">
        <property type="entry name" value="LGIC_TM_5-HT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01709">
        <property type="entry name" value="5HT3ARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01708">
        <property type="entry name" value="5HT3RECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 27" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 27" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 27" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 27" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="418"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="28">
        <location>
            <begin position="17"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="29">
        <location>
            <begin position="230"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00037540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00061202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00061864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00068982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00078864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00080492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00083210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="444" mass="50890" checksum="2AFBE70EFD42F393" modified="2018-12-05" version="1">RDPPSSPGRFANATLVRLADFLSAGYKKGVRPVKDWRTSTIVAIDLMVYSILNVDEKNQVLTTYVWYRQSWTDEFLVWNPEDFDEVKQVSLPTANVWVPDILINEFVDVGKSPDIPYVYVTHEGLVQNYKPIQVVTACTLNIYNFPFDVQKCSLTFQSWLHTIDDINITLMRSPEKLRDDKSVFMNQGEWELLHILSNYKIFSVDNDDYYAEMKFHVVIRRRPLFYTVNLLLPSVFLMVMDIVGFFLPPDSGERVSFKITLLLGYSVFLIIVSDTLPATAIGTPLIGVYFVVCMALLVISLTETVLIVRLVHKQDLQPPVPHWVKYLVLERAPITPLSHSAAQCSLHHTCEIGQRLSQQDREGGMLGLRLAPSRDNTPPVMDNILQEVTAIRQFLEKRDRCREIAKEWLQVGYVLDVLLFRVYLVAVIAYSITLGTLWSVWQVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKZ0</accession>
    <name>A0A3B5MKZ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ankyrin repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKZ0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020184"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020431.1">
        <property type="protein sequence ID" value="ENSXCOP00000020184.1"/>
        <property type="gene ID" value="ENSXCOG00000015128.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182928"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021832">
        <property type="entry name" value="ANKRD13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055285">
        <property type="entry name" value="ANKRD13_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12447">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12447:SF25">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 13C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11904">
        <property type="entry name" value="ANKRD13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ankyrin repeat" evidence="7">
        <location>
            <begin position="12"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="180" mass="20693" checksum="C0B2259584029F13" modified="2018-12-05" version="1">MFKDLCSLSSRKRREHLSEEDIMRNKAIMESLSKGGSISEQNFEPARRQSLTAPAPNTISWEEYITAEHGKPPHLGRELVCKESKKNFKATVAMSQDFPLGIESLLNVLEVIAPFKHFNKLREFVQMKLPPGFPVKLDIPVFPTITATVTFQEFRYDKFDESIFLIPAEYKEDPSRFPDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKZ1</accession>
    <name>A0A3B5MKZ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BCL6 corepressor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BCOR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKZ1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025054"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025359.1">
        <property type="protein sequence ID" value="ENSXCOP00000025054.1"/>
        <property type="gene ID" value="ENSXCOG00000018705.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000235020"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047144">
        <property type="entry name" value="BCOR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24117">
        <property type="entry name" value="AGAP007537-PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24117:SF8">
        <property type="entry name" value="BCL-6 COREPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="253"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="327"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="334"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="370"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="447"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="474"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="494"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="580" mass="62729" checksum="9B681A55832ABF37" modified="2018-12-05" version="1">MISTLHSSWVVPSPSPSHAQHTSGLPPSPRSNADLGLSQAKGASPSVIKNKGLERVLPQQRSSSCPRIGEPNLSVPSSQHSGCSLISSSRALSPKPNGEWVKHSPSQSVHHREGTEKSAQTNKRSEPTPDLSSYKRPCLENGHPPPHLFLSQSEAYLNHSLAYANRYLQYPIPDGLALHSVPISGKGPVYPNPVLLGNNGLYPTHLAGKHPLTYPNLPAGPGTEYLTYNSQEMAHPLMQTLSDSKLTDRADATLKPRGQDRPRGAVEEGGIHRDHSAGKETGEGNQMKHDREVGVQGQGGSQSKNLPSSAASREKIVCIDLVHSDTDIEPTPTVYKQPSAETSNKGNQNECHSSQNLTNTDSETKHQAHLSHSGQTPNLKVNNTDGQQEALFGKGKIPQETPCLVEVSRTSQTRPELSDAEESTEESSPSPDPGDQDQSTLRCARTSGERSSGKDKRDGGSCAPLAQHNTNAVKECEREAEKEDNIVDLGESQAEGDDEEEEDEGTQASSKNSRRSSLAKRIANSSGYVGDRFKCVTTELYADSSKLSREQRALQVSPQVYLYTTDCIGLLSCHDQTFQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLB9</accession>
    <name>A0A3B5MLB9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Heat shock protein 12A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027880043.1">
        <property type="nucleotide sequence ID" value="XM_028024242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLB9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020304"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020552.1">
        <property type="protein sequence ID" value="ENSXCOP00000020304.1"/>
        <property type="gene ID" value="ENSXCOG00000015224.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114148761"/>
    <dbReference type="KEGG" id="xco:114148761"/>
    <dbReference type="GeneTree" id="ENSGT00940000154551"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10229">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA12"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187">
        <property type="entry name" value="ALPHA KINASE/ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187:SF5">
        <property type="entry name" value="HEAT SHOCK 70 KDA PROTEIN 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="568" mass="64045" checksum="B25705D624F4DBF8" modified="2018-12-05" version="1">MGDSFIIAIDFGTAYSGYAFSITSRGDEIDPYLKVWGEEVGLETPKTPSCILFDKQEEFMSFGYVAKEDYLSKKGQEARDMVFFDCFKMALYGNDINRDLMIKAANGKSMKALKVFTEALRFLKYDALETINSNAKGRKFIPSDFTWVLTVPAIWDPSAKQFMREAAMQAGIVSEGNQDKLVIALEPEAASVWCKKLPSDGFITQNIGRNTLDQSPGTQYIVVDCGGGTIDITVHEVLEGGALKELHKASGNDLGGQNVDRKFKQFLREIFCDGVWDKYEENYPGEVQKIMYDFTLFKKGDKDIEITCSLNLGRLAQTKKDIEKFLEGVQGASWDEGSIRISKEKLRSFFDESLQGIVKSLDNILRRGYRIGYILLVGGYADSQMLQRHITEEYIGECNVLCPFRPQEAILKGAVMFGRNPAVVTSRKSAFTYGLAVAQRFDASNHRADKKYTNQDGEWCDDIFMKLVEINEDVGWNQTKSFSLSPTSSTQTARNFYFYRTQYKSPKYVDEEGVEKIGGFVLDSPNTERGLDREAKLNINFGFTEMKATATDIDSGSTKSIKLDFMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MM10</accession>
    <name>A0A3B5MM10_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MM10"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025751.1">
        <property type="protein sequence ID" value="ENSXCOP00000025444.1"/>
        <property type="gene ID" value="ENSXCOG00000019007.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000173749"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="128" mass="14430" checksum="C5AB10787FE0E0DF" modified="2018-12-05" version="1">GSMTGLKKEEESVAICLTRSRQNKVHTLSSSRSCAMLDAVHRARLKQLRGPRSQAPKVPKASHACLQCPAAYNDCDALIMHQLRHIDGKHWPCPLCSKTFFRLRNVRNHIRTHDPKLYKCRSCIAAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MN62</accession>
    <name>A0A3B5MN62_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">GNAS complex locus</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MN62"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025859"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026170.1">
        <property type="protein sequence ID" value="ENSXCOP00000025859.1"/>
        <property type="gene ID" value="ENSXCOG00000019325.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155271"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007191">
        <property type="term" value="P:adenylate cyclase-activating dopamine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007606">
        <property type="term" value="P:sensory perception of chemical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000720">
        <property type="entry name" value="Guanine nucleotide-binding protein G(k) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF233">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(OLF) SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0807">Transducer</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="50"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="86" mass="9699" checksum="3ED83F139D1E26B3" modified="2018-12-05" version="1">MGCLGNSKTEDQRIDEKAQREANKKIEKQLQKERQAYKATHRLLLLGAGESGKSTIVKQMRILHVNGFNAEAAARFNQDLKIIMMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQS0</accession>
    <name>A0A3B5MQS0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">GATA binding protein 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027857847.1">
        <property type="nucleotide sequence ID" value="XM_028002046.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027857849.1">
        <property type="nucleotide sequence ID" value="XM_028002048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQS0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023531"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023812.1">
        <property type="protein sequence ID" value="ENSXCOP00000023531.1"/>
        <property type="gene ID" value="ENSXCOG00000017570.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114135098"/>
    <dbReference type="KEGG" id="xco:114135098"/>
    <dbReference type="CTD" id="30481"/>
    <dbReference type="GeneTree" id="ENSGT00940000166313"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043009">
        <property type="term" value="P:chordate embryonic development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048821">
        <property type="term" value="P:erythrocyte development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030225">
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0060215">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010725">
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        <property type="project" value="Ensembl"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Transcription factor GATA-3"/>
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        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF190">
        <property type="entry name" value="ERYTHROID TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003027">
        <property type="entry name" value="TF_GATA-1/2/3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="14">
        <location>
            <begin position="239"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="GATA-type" evidence="14">
        <location>
            <begin position="293"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="GATA-type 1" evidence="11">
        <location>
            <begin position="245"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="GATA-type 2" evidence="11">
        <location>
            <begin position="299"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="86"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="106"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="426" mass="45782" checksum="21822B903ED73554" modified="2018-12-05" version="1">MEDSSDQSHWASPTLLSSDPLAGFPSDPGLLTAGEEGEAFFSAADADYAGLSSFFPNPSHSRAPSSYRHSSVRQVYTSPGLLSNLQLLDGPPSHPLSSPYSPAASTWSSSPLTKTPLHSHTPTSLYSPGMASSFTASRDGYPSPGREGRESPRLQEALKAERLSPLGGSGASSNFLNLTPAAGSMYTPSSHSHPHMLSPYSSYMSGPQDYGSPAFYSSAGAWISPSYSPKLRNKLRPTTPEARECVNCGATATPLWRRDGTGHYLCNACGLYHKMNGQNRPLIRPKKRLIVSKRAGTVCANCHTSTTTLWRRNASGEPVCNACGLYFKLHNVNRPLTMKKEGIQTRNRKVSNKNKKSKKAAMLEPYLDAVQPPPLDDNGGPFSLGPGTLLTYSHTPHLLPTPTPLHPSTGLPYAHHLNSGMVPTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MR87</accession>
    <name>A0A3B5MR87_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Indoleamine 2,3-dioxygenase 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the indoleamine 2,3-dioxygenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027888602.1">
        <property type="nucleotide sequence ID" value="XM_028032801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MR87"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021999"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022266.1">
        <property type="protein sequence ID" value="ENSXCOP00000021999.1"/>
        <property type="gene ID" value="ENSXCOG00000016435.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114154044"/>
    <dbReference type="KEGG" id="xco:114154044"/>
    <dbReference type="CTD" id="3620"/>
    <dbReference type="GeneTree" id="ENSGT00940000164380"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033754">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004833">
        <property type="term" value="F:L-tryptophan 2,3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.480:FF:000003">
        <property type="entry name" value="Indoleamine 2,3-dioxygenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000898">
        <property type="entry name" value="Indolamine_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037217">
        <property type="entry name" value="Trp/Indoleamine_2_3_dOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28657">
        <property type="entry name" value="INDOLEAMINE 2,3-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28657:SF4">
        <property type="entry name" value="INDOLEAMINE 2,3-DIOXYGENASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01231">
        <property type="entry name" value="IDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140959">
        <property type="entry name" value="Indolic compounds 2,3-dioxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2 9" id="KW-0349">Heme</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0823">Tryptophan catabolism</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="364"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="binding site" description="proximal binding residue" evidence="9">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600898-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="422" mass="47351" checksum="478AD49866D61ABF" modified="2018-12-05" version="1">MASAETERRAPFSLDSYYVSEELGFVLPDPLENLPSYYQPWMDIALRVPELVHSHDLRSHINKMPLLSTKFLQNHRELRLAHLVLSMVTMGYVWQDGENDTVEMLPRSLAVPYWEVSQRLGLPSILTHADAVLANWRKKDPEGPFDMENLELLVRLPGGDSVKGFFLVTLLVELAAVPALRSIPAIINGVRCGDTDAVTKALENIGQSIQDMNDALKLMHVYVDPSVFYGIMRIFLSGWKDNPCMPKGLVYEGVQTEPIGFSGGSAAQSSLLHCFDELLGVKHEEKSRAFLTRMRNYMPPAHQKLIQDISLQPSLRGFVQQLANEDLSKAFDDCVSKLLALRSYHINVVSRFITVPAARARQLRTNSHDSEGEMVSRAPTSLEERGTGGSGIMTFLKTVRDKTKDVLLHETSKKTVHENCHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MS36</accession>
    <name>A0A3B5MS36_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MS36"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022299"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022572.1">
        <property type="protein sequence ID" value="ENSXCOP00000022299.1"/>
        <property type="gene ID" value="ENSXCOG00000016663.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166179"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="19377" checksum="FEAB26335AF2A66C" modified="2018-12-05" version="1">MHGVLEFLYCGLLTPCPGLEPMELIVLANRLCLPRLVALTEQHAVDELLQLAVNGVDIDGQVLAYLEVSQFHNAKQLSTWCLHHICTNYNSICRKFPKDMKAMSPENQRHFEKQRWPPVWFLKEEDRYLRSQKEREREEEILRKQHTKRGWCFWRHPSSSPHVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MT29</accession>
    <name>A0A3B5MT29_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">TGF-beta family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MT29"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022654"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022930.1">
        <property type="protein sequence ID" value="ENSXCOP00000022654.1"/>
        <property type="gene ID" value="ENSXCOG00000016929.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160140"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035239">
        <property type="term" value="P:tube morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13766">
        <property type="entry name" value="TGF_beta_GDF5_6_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000001">
        <property type="entry name" value="Bone morphogenetic protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF43">
        <property type="entry name" value="GROWTH_DIFFERENTIATION FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00669">
        <property type="entry name" value="INHIBINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11" id="KW-0339">Growth factor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="domain" description="TGF-beta family profile" evidence="12">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="116" mass="12865" checksum="E7E366C7B28821CB" modified="2018-12-05" version="1">EIAAVSHLGGKQPVCSKKALHVNFKELGWDDWIIAPLGYEAYHCEGVCDFPLRSHLEPTNHAIIQTLMNSMDPNSTPPSCCVPTKLSPISILFTDSGNNVVYKQYEEMVVEQCGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVB2</accession>
    <name>A0A3B5MVB2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier family 35 member B1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleotide-sugar transporter family. SLC35B subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVB2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023469"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023750.1">
        <property type="protein sequence ID" value="ENSXCOP00000023469.1"/>
        <property type="gene ID" value="ENSXCOG00000017527.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157900"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005459">
        <property type="term" value="F:UDP-galactose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005460">
        <property type="term" value="F:UDP-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="254"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="335" mass="37156" checksum="6F73A8517A36A768" modified="2018-12-05" version="1">VSVNLPGLVEDSMAAGKGAGKPVSLWDNLRIRFIVCFFGVFVCYFYYGILQETITRGNYGGERFTFARTLVFIQCIINSAFAKILIQCFESSKVDHTKSWLYGLCSLSYLGAMVSSNSALQYVNYPTQVLGKSCKPIPVMILGVTILRKKYPLAKYLCVLLIVGGVALFLYKPNKAAAADDHIFGFGEILLLVSLTLDGLTGVAQDHMRARFQTGANHMMLNINMWSTLVLGLAVLWTGEVWRFLSFAEQYPSIFYNILLFGLTSALGQTFIFMTVVYFGPLTCSIVTTTRKFFTILGSVILFGNVMTDMQWIGTILVFLGLGLDAKFGKSPKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVC8</accession>
    <name>A0A3B5MVC8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SOCS box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVC8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023489"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023770.1">
        <property type="protein sequence ID" value="ENSXCOP00000023489.1"/>
        <property type="gene ID" value="ENSXCOG00000017543.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000178579"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SOCS box" evidence="2">
        <location>
            <begin position="74"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="132" mass="15697" checksum="31045FA7E6417CFB" modified="2018-12-05" version="1">MVLRWTFSVKTVGHIMSRSLSAQFCEVITVYWLKDLSGHVVRVLLDYVDHVMLCSKLKAVLMEQNQWTDICRLQENPRYLQHLCRLRIRRCLGRLRLRSPVFMSFVPLPERLKDYILYREYDLLGRQRGGPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MX04</accession>
    <name>A0A3B5MX04_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">INO80 complex subunit F domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027867962.1">
        <property type="nucleotide sequence ID" value="XM_028012161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MX04"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026062"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026375.1">
        <property type="protein sequence ID" value="ENSXCOP00000026062.1"/>
        <property type="gene ID" value="ENSXCOG00000019469.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114141565"/>
    <dbReference type="KEGG" id="xco:114141565"/>
    <dbReference type="CTD" id="29844"/>
    <dbReference type="GeneTree" id="ENSGT00940000167238"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097190">
        <property type="term" value="P:apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056513">
        <property type="entry name" value="INO80F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033555">
        <property type="entry name" value="TFPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35084">
        <property type="entry name" value="TCF3 FUSION PARTNER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35084:SF1">
        <property type="entry name" value="TCF3 FUSION PARTNER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24245">
        <property type="entry name" value="INO80F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INO80 complex subunit F" evidence="4">
        <location>
            <begin position="62"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="140"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="199" mass="22143" checksum="5594226ECEF82B11" modified="2018-12-05" version="1">MMEDFSGLALPPLFGGHILEAELEPGGVELGPGEVVLDPDPGGSELLESTPQEDEERRALDKRKCLVLSRRCKEIEQVNQKILGRLHQVQRITRRLKKERRFLMKALDAHGDDYRKTPLTILLEEEPGAHLDSAAGVEEEKLNGLSGSSATLHHAAGPKKKRHRISRQDKDKDQQIEPDVSVLAETHFGEMPSPTSLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MY77</accession>
    <name>A0A3B5MY77_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MY77"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025485.1">
        <property type="protein sequence ID" value="ENSXCOP00000025180.1"/>
        <property type="gene ID" value="ENSXCOG00000018808.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064692"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF7">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="429" mass="47907" checksum="CCFC016D51A7304D" modified="2018-12-05" version="1">MDVPPGGLTQRAATGGPPSLKQQKLNFGAKSVSERELKQLVGQYVVEEMMPLSAVNSPAFCAIIHRIPTTINSELPHRGAFLPYLDKENAEMERNLKAALNEVDFVATSKRNKAALACRRIRGRHTYDVIGAEIENIHSSYGLLNKVVATVTDNGSSFVKAFKVFQPVTESDDETEEESTKTDDDDVTFVNPTEILSTENEGDGQLSLPPHHRCASHTINIISTSDVEKYLTFHAESKAVYRSSIAKCTALWTKSSRSTLASEAVEEVSKRKLLVPTSTRWNSFLDAVKRVAEIPMNDLNMLCCKLGVKCFIDREYQFLHEYCTVMKPLTEALDKATVLMGLYYPHLKLWFIQKQLDRVLSTSSSSNCSAHFNLFLPPTLLPFSSSHLFLSLCPLTHVVSSDLFRIIISKITTQSSLNCQKNKIRSVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MYL6</accession>
    <name>A0A3B5MYL6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BCL9 transcription coactivator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">BCL9</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BCL9 family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000025440"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025747.1">
        <property type="protein sequence ID" value="ENSXCOP00000025440.1"/>
        <property type="gene ID" value="ENSXCOG00000019006.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000110915"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990907">
        <property type="term" value="C:beta-catenin-TCF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015668">
        <property type="entry name" value="Bcl-9/Bcl-9l"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024670">
        <property type="entry name" value="BCL9_beta-catenin-bd_dom"/>
    </dbReference>
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        <property type="entry name" value="B-CELL CLL_LYMPHOMA 9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15185">
        <property type="entry name" value="BCL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11502">
        <property type="entry name" value="BCL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B-cell lymphoma 9 beta-catenin binding" evidence="5">
        <location>
            <begin position="456"/>
            <end position="493"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
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        <location>
            <begin position="76"/>
            <end position="212"/>
        </location>
    </feature>
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        <location>
            <begin position="242"/>
            <end position="278"/>
        </location>
    </feature>
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        <location>
            <begin position="323"/>
            <end position="576"/>
        </location>
    </feature>
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        <location>
            <begin position="794"/>
            <end position="1082"/>
        </location>
    </feature>
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        <location>
            <begin position="1214"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1331"/>
            <end position="1353"/>
        </location>
    </feature>
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        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
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        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
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        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
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        <location>
            <begin position="183"/>
            <end position="196"/>
        </location>
    </feature>
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        <location>
            <begin position="197"/>
            <end position="212"/>
        </location>
    </feature>
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        <location>
            <begin position="242"/>
            <end position="277"/>
        </location>
    </feature>
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        <location>
            <begin position="323"/>
            <end position="356"/>
        </location>
    </feature>
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        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="430"/>
            <end position="443"/>
        </location>
    </feature>
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        <location>
            <begin position="451"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="474"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="497"/>
            <end position="511"/>
        </location>
    </feature>
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        <location>
            <begin position="528"/>
            <end position="545"/>
        </location>
    </feature>
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        <location>
            <begin position="933"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="965"/>
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        </location>
    </feature>
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        <location>
            <begin position="1010"/>
            <end position="1024"/>
        </location>
    </feature>
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        <location>
            <begin position="1050"/>
            <end position="1063"/>
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    </feature>
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        <location>
            <begin position="1222"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1257"/>
            <end position="1275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11502"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025440.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1475" mass="153785" checksum="094AE7039FCCD36A" modified="2018-12-05" version="1">MLEVQEERPAAAGTAATHFTKKERAKKEREEAKDGRGNLSNITNPVSGSRNVRAKAALTHTGSPHQLITPPCSVVLGAPSMHSNRLKNSPSTNTQSPKPKTEAMVRSPPVMSPSTAAQMDSKMPNQSKPGSAGSQSQPSSCDPKTMGAKAAQNVSGGMGLKNGQGLTTGPSSKVKAKRERSTSVESFEQPESGTPTNEDKDNSRAKRICVAERRQPYSGTDWCSGGESDEDDRGFFVNAQDTHSTSNAGLSRSSTPSHNTLGGQGSTTEPTSGQKSGSKLVRVFSTEMANAADAVLTGHTENIIDFHIKNFKDKAHLLLNNTSGVLRNDSNPPQQPPSHAQDQSHQPGSKPSLSGMSEQAPPQSSSQGSQPSVLPQEGSSSAGTESKNIPGSSPGNPSAPVDQAPVAQPETGLNPPTSVEGGPGGGTSGSGLTPQQQQQQQQLAQELLNMEANTEGLSQEQLEHRQRSLQTLRDIQRMLFPDDRDAPPPGPQQAHVGPHDGGPDGPPRRPEQGPLQAMMAQSQSLGPPGGPGGPRPQGPPFGPPHGPREMPLFPTDDMVPPGGPGGPGGCGEGDQMTPEQVAWLKLQQEFYEEKRKKQELQHRPLPPDMMHPGPRGMMRGPPPPYQMGPGEMWGGPGGPPEHYPERMGMGPGPRGMGPHMQRMPGFSGMMNPEMEGLPRPGMGWPDDMPPRMAEARGFPGGPAGMFPGPGARGERFPNPQSVQEAMFHQGMSGEKGLPPGMMMDVQRMMGHQRGGMEPGNGMGMFPRMPVDGPMSPSSRLQAMGGREMGPEFGIGPGPHMHPSKLRDGPMNMSPDEMMRMRTGGGPPMENMGPQGRPMQVPPFPEQTPADFPMGPGRPFPGGPGTLRGPHADPAFGPEHRSTPSGGNGRINHLSSAGGPAQGQRGRKPADLNVQAGGGNSPSVNPLKSPPLRQVQSPMMGSPSGNLKSPQTPSQLAGMLSGPAGPSAPPAPPTSAPMKSPHSMMGSAGASPVHMRSPSLPNPSPGWASSPKPPMQSPGVPPQGGKPPLSITSPNMMGGMEPGNGPPSAPPSSGAPSGSMSLPGNVPSGSPYTIPPEPTLSQNPLSIMMSRMSKFAMPSSTPLYHDAIKTVASSDDDSPPARSPNLPSVNNMAMNHQGNPRMMGPGNTVPMPALSPLGMNPLGSQPLSHGMPPQMPSPNAPNMGPGMMPHGMMMQPNPQDPGMPNPQMMPQGRMGYPHRNQGYPLTQSPSQQGPFSPHNGPGPQGFPGHSMGFQGEPGPMGGRMGNMPHGGGGDGGMCKPNTPGGPEFNNMQGGFGDADLHEVMRPGASGIPEFDLSRIIPSEKPSQTLSYFPRGGGENPGGKPPHPSGFPMQAMMADGPPRMGMPMQGMGGMSGGPGGGMGPQDMPMGNPGHNSMRPPGFMGQGMMGPQHRMMSPGGPGGMMQGRQMAHPGPGGSPNMMMSLQGMGGPPQQTMMMGGQMRPRDMDMGFSPGHGMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZ90</accession>
    <name>A0A3B5MZ90_9TELE</name>
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        <recommendedName>
            <fullName evidence="1">Dipeptidylpeptidase IV N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSXCOT00000027775.1">
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        <property type="gene ID" value="ENSXCOG00000020490.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="INACTIVE DIPEPTIDYL PEPTIDASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
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    </dbReference>
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    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="1">
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            <begin position="35"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="545" mass="62204" checksum="94A8E8B75D9B35B2" modified="2018-12-05" version="1">RDGDVIRFNFALNETEVILKNTTFVSFKVAKYSLSPDLKYALFAYDVKQVYRHSYTASYIVYNINTREVWELNPPEVQNSVLQHAAWGRQGRQLIYIFENNIYYQSDVKSSSLRITSSGMEGAIFNGIADWLYEEEILHSHLAHWWSPDGERLAFLRIDNTLVPNMALPQFTGSTYPRGIQYPYPMAGQRNPTVKLFVVNLFGITNTQELHPPDQLRLREFYITMVKWINHTRLAVRWVNRPQNASLLTVCEATTGVCHQRHEESSDTWLTSQEPLFSEDLSRIFLIMPVRHGGHRDFHHVAMFSKKSRSDPVEVRHLTSGNWEVIRIVAYDESKNLIYFLSSEKAPQQRHLYSLSTNGSFSKECLTCDMKEDCLFFDADISPNAQNAILYCQGLRHSPLPLFCNHLSLAAYFILESNVPLRSALDARRTSKTDFLNSQAVSKSEMFLGRGSAPGEQAVTEKFGLNWDLVLAVSEQIIVAQLDGRGPGFGGQRFLVKLPYVDRTRIGGYGGYITLMMLDSERLIRCAAAQEKYMFNTCAAEPPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZC1</accession>
    <name>A0A3B5MZC1_9TELE</name>
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        <submittedName>
            <fullName evidence="3">Family with sequence similarity 117 member Ba</fullName>
        </submittedName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MZC1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027826.1">
        <property type="protein sequence ID" value="ENSXCOP00000027492.1"/>
        <property type="gene ID" value="ENSXCOG00000020528.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183046"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026642">
        <property type="entry name" value="Glcci1/FAM117"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14972">
        <property type="entry name" value="AGAP011572-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14972:SF6">
        <property type="entry name" value="PROTEIN FAM117B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15388">
        <property type="entry name" value="FAM117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="41"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="72"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="222" mass="24103" checksum="CBFD032DA1A1841F" modified="2018-12-05" version="1">MRDKATQSPMPKTILIPIPISKSSAPRFRNSVEGLNQEIERIIIRDTPEKDETIVPQDVPDGHRAPPPVPPQRSSSTRSIDTQTPSGGGLSGNHSNCSSRPDSISPSYLSVLNDTAGGSPYDEKLSPCSPLPKYAASPRPNNSYMFKREPPEGCEKVRVSEESPKPLEEIPPFLCPDRNKVNFIPNSGSAFCLVSILKPLLPAPDLNFRPGLGLRSLSPSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N0A6</accession>
    <name>A0A3B5N0A6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5N0A6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026465"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026786.1">
        <property type="protein sequence ID" value="ENSXCOP00000026465.1"/>
        <property type="gene ID" value="ENSXCOG00000019764.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000113054"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019902">
        <property type="term" value="F:phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031410">
        <property type="entry name" value="SAXO4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34349">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34349:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15691">
        <property type="entry name" value="PPP1R32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="222" mass="24826" checksum="0B3E9DE2B2734C8C" modified="2018-12-05" version="1">MTDTLLVAVSGRMCFDSCLGAPVASGFVSNQRPVLSYQPSLDLVDNPHFGVWLADNFVTQNMWYYQRPTYSDGSMALPRLLNKPMTSGFHQVSSVCHTEPVVEKTEYQRVFVPRRLPPVSRHMNMGPKLETGCTEGEDLQFITFRDKKYATVDPRLTSSTVMMKDYLSPTFKQGNECLPGICSCSCRETGYTRGANALLVDPVSPSILQLVFISNCCYCTNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N1V8</accession>
    <name>A0A3B5N1V8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Spire-type actin nucleation factor 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the spire family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N1V8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027835"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028171.1">
        <property type="protein sequence ID" value="ENSXCOP00000027835.1"/>
        <property type="gene ID" value="ENSXCOG00000020756.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003058"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051639">
        <property type="term" value="P:actin filament network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036089">
        <property type="term" value="P:cleavage furrow formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051295">
        <property type="term" value="P:establishment of meiotic spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040038">
        <property type="term" value="P:polar body extrusion after meiotic divisions"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22186">
        <property type="entry name" value="WH2_Spire1-2_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22065">
        <property type="entry name" value="WH2_Spire_1-2_r1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011019">
        <property type="entry name" value="KIND_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029901">
        <property type="entry name" value="Spire"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21345:SF5">
        <property type="entry name" value="PROTEIN SPIRE HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21345">
        <property type="entry name" value="SPIRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16474">
        <property type="entry name" value="KIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00750">
        <property type="entry name" value="KIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00246">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51377">
        <property type="entry name" value="KIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="KIND" evidence="15">
        <location>
            <begin position="21"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="132"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="417"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="418"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="434"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="594" mass="66882" checksum="1B10637D41FBAE43" modified="2018-12-05" version="1">MARSTNRSAEVGDPRVTPRELSLEEVLKSYEQPIKEEQAWAVCYQCCSGLRVPRPPPGRPGRVKDPSSILLHRDGTDLWCEDSPDGCEGSNAERLVQSLGVAIYRALDWGLDDNEERELSPQLERLIERMAGGDQGGEGVSSAGSGATDEGYSGQEEEEDEDEEQERAIRPVCWFRQVVVLCASRLANPSLAPEHYQAVCRALFVETLELQTFLIKIKDAKEMLKKISSEEALEDRSTAELDGLKHRDWARLWVQLMKELRQGVKLKKVQEQPFNPLPTEFSLTPFEMLMQDIRARKFQLRKVMVDGDIPTRVKRSAHELILDFIRSRPPLKPVSERSLPPPPPRQQSLHERVLAEIKQERKLRPVVPHTARPFGSLPCLAQTCPCDVKSTSCIDLSVSEPRPSLRPRILLKAPTLAEMEEMSISEEDESPDGGDMRRTESSPAPLKRDRSFSEQDLAFLRGDMLPSLSESARLGETVRLQDVRPRSRTLTASFPVYGWAPPSPARLSLSSVDEATEGLETTSDSRSGDQWMEEFSHPVESLALTLDEIINVRRVLVKAEMEKYLQSKELYSNLKRGKVAALVSLLLLPLLCFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KJF0</accession>
    <name>A0A3B5KJF0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Apple domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KJF0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000263"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000270.1">
        <property type="protein sequence ID" value="ENSXCOP00000000263.1"/>
        <property type="gene ID" value="ENSXCOG00000000140.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158569"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01100">
        <property type="entry name" value="APPLE_Factor_XI_like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000177">
        <property type="entry name" value="Apple"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33946">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33946:SF4">
        <property type="entry name" value="COAGULATION FACTOR XI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00024">
        <property type="entry name" value="PAN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14295">
        <property type="entry name" value="PAN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00005">
        <property type="entry name" value="APPLEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00223">
        <property type="entry name" value="APPLE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57414">
        <property type="entry name" value="Hairpin loop containing domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017188055" description="Apple domain-containing protein" evidence="3">
        <location>
            <begin position="16"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="4">
        <location>
            <begin position="23"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="4">
        <location>
            <begin position="204"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Apple" evidence="4">
        <location>
            <begin position="280"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="371" mass="42390" checksum="22E1AA59CA98B527" modified="2018-12-05" version="1">FRFLHLLIFQTYSFCSFSNFSTCIQGFLQNVDFPGSDVVDLYSPSVEHCQELCTQHPSCLFFSFNRPDKSSGRFRCYLKSSSSGHPQSQVLLLGVSSGYSLKACGLEPTPCFPQVFEDADFHGGDYRTLFTVNFEECQRVCTLDPVCQFFTFVMGNFSSPDVRYKCHLKFSWYVPRPVAVVLKAGVISGFSQKSQLTQHFATVCRSHLFSNTDLRGNDFLESQAVSPEHCQFLCSVHPQCTYFSFSSNDFRCFLKNNPNSMVARTKSNFTSGISSHLCQIDESWAKLTYKDIEFRGSDFRSEVVTCVDICQEKCTEDPNCQFYTYHNGSFFHPDHRHRCYMKRVITMPTPSQVKKVDNVKSGFTLRNCRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KPE8</accession>
    <name>A0A3B5KPE8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KPE8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001998"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002028.1">
        <property type="protein sequence ID" value="ENSXCOP00000001998.1"/>
        <property type="gene ID" value="ENSXCOG00000001611.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="P:RNA catabolic process"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNase_H2_subunit_C"/>
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        <property type="entry name" value="RNase_H2_suC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47063">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47063:SF1">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08615">
        <property type="entry name" value="RNase_H2_suC"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="135" mass="15158" checksum="1618991F5CE65112" modified="2018-12-05" version="1">MTCHGVTRLHLSSLKRAEKVPVHLMPCEIEHNGLAQVSQYFTATTKENKKEKTVSFRGRGLKGQEISCPQGYTGLVLKEVDRHGSDQEDRTVKVTSVFDKLTYWNLETPPTSDDTVVMAMDWPELAEAIHGTVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KPV5</accession>
    <name>A0A3B5KPV5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SPIN-DOC-like zinc-finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KPV5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000506.1">
        <property type="protein sequence ID" value="ENSXCOP00000000497.1"/>
        <property type="gene ID" value="ENSXCOG00000000437.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182812"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040647">
        <property type="entry name" value="SPIN-DOC_Znf-C2H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913">
        <property type="entry name" value="EPM2A-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913:SF11">
        <property type="entry name" value="EPM2A-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18658">
        <property type="entry name" value="zf-C2H2_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="SPIN-DOC-like zinc-finger" evidence="3">
        <location>
            <begin position="106"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="633" mass="72478" checksum="099AF2C06750C297" modified="2018-12-05" version="1">ALKSDPDLIELEINQIKLTGKTFGDSDMKPLLEILESSVSKVKELRFVCFLSLSLSLSLSLSIYIYIYICIYIYIYVFVSITVKLTFRYPSKMAKRKVDTENRGFQTRWESEYMFTEVAGKPVCLLCGESVAVLKEYNLRRHYETKHADKNKNMDMEQRLQKAEELKRGLKSRQALFKKAKSQVCPEKRQLFLNVSLSRNTIAERVVQLSINLKEQLVKKGKDFIAYSLAVDESADISDIAQLSIFIRGVDSSLSVTEELLALRPMHGTTTGHDLYEEVSRCVNEMELPWEKLVGLTTDGAPAMCGHRSGLVAKIREKMQEENATGELTAYHCIIHQEALCGKALKMEHVMSIITRAVNFIRAKGLNHRQFKVFLTELETEHGDLPYHTEVRWLSQGKVLQRCFELREEICLFLDSKGKDTTQLRDEMFLCEMAFLCDITSHLNTMNLQLQGRDRVISDMYSTVKAFKTKLTLWETQMRKENLSHFPSCQTMKEKLSTSAFPSAQFADKIGMLSADFRRRFADFEAQKNRFELLGNPFGVDVESSPPNLQMELIDLQCNDALRAKYAAVGAAEFARFLPDTMPQLRIQAAQTLSMFGSTYLLSYYSALPLCKLHASYTLQHYSTQKDQLLHTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRN5</accession>
    <name>A0A3B5KRN5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Membrane</location>
            <topology evidence="2 17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027896363.1">
        <property type="nucleotide sequence ID" value="XM_028040562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRN5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002773"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002810.1">
        <property type="protein sequence ID" value="ENSXCOP00000002773.1"/>
        <property type="gene ID" value="ENSXCOG00000001805.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114158744"/>
    <dbReference type="KEGG" id="xco:114158744"/>
    <dbReference type="GeneTree" id="ENSGT01030000234549"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000090">
        <property type="entry name" value="Long-wave-sensitive opsin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001760">
        <property type="entry name" value="Opsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000378">
        <property type="entry name" value="Opsin_red/grn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027430">
        <property type="entry name" value="Retinal_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00238">
        <property type="entry name" value="OPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00575">
        <property type="entry name" value="OPSINREDGRN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00238">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0157">Chromophore</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 17" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3 17" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="13 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="5 17" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="15 17" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="16" id="KW-0844">Vision</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="85"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="216"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="263"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="67"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="357" mass="40002" checksum="7DFBFF7694AEBEE2" modified="2018-12-05" version="1">MAEDWGKQVLAPWKNNEETTRGSAFTYTNSNHTKDPFEGPNYHIAPRWVYNITTVWMCFVVVLSVFTNGLVLAATAKFKKLRHPLNWILVNLAIADLGETVFASTISVCNQFFGYFILGHPMCVFEGYIVSTCGIAALWSLTVISWERWIVVCKPFGNAKFDAKWATAGIMFSWVWSAVWCAPPVFGWSRYWPHGLKTSCGPDVFSGSEDAGVKSYMIVLMITCCIIPLAVIILCYLAVWLAIRDIAMQQKECESTQNAQKEVSRMVIVMILAYCICWGPYTFFACFAAANPGYAFHPLAAAMPAYFAKSATIYNPVIYVFMNRQFRTCIMQLFGKEVDDSSEASTSKTEVSSVAPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRP7</accession>
    <name>A0A3B5KRP7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027880669.1">
        <property type="nucleotide sequence ID" value="XM_028024868.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027880677.1">
        <property type="nucleotide sequence ID" value="XM_028024876.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027880686.1">
        <property type="nucleotide sequence ID" value="XM_028024885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRP7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001157"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001173.1">
        <property type="protein sequence ID" value="ENSXCOP00000001157.1"/>
        <property type="gene ID" value="ENSXCOG00000000953.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114149217"/>
    <dbReference type="KEGG" id="xco:114149217"/>
    <dbReference type="CTD" id="58487"/>
    <dbReference type="GeneTree" id="ENSGT00390000016589"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14706">
        <property type="entry name" value="bZIP_CREBZF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000125">
        <property type="entry name" value="LAS1-like, ribosome biogenesis factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="3">
        <location>
            <begin position="117"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="82"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="133"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="256" mass="28509" checksum="7FAD5899DAB62BAB" modified="2018-12-05" version="1">MITRRRSRAMEVPSVDVEEVDVMENVHDLPNPSDPLAADDTDPSGMELDDLFEDLKWTLEKDSSSLLFGVDLADLEAHSDINQNSGFDASSRSSPDITSSGSRMKNRQNQSNHVINKNAIAARLNRLKKKEHVNTLEKQVGVLSTENAVLKQENCQLTKRVEELEDETRYLRAVLANESMLAQLLSRLSGVNGMKLSSSLFQGPRSNEHDYALPRKRVKVEEKETSGGVCLHVDKNHVSVEFCPKCAESASTSLKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRU9</accession>
    <name>A0A3B5KRU9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRU9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002833"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002870.1">
        <property type="protein sequence ID" value="ENSXCOP00000002833.1"/>
        <property type="gene ID" value="ENSXCOG00000002250.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016928"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055074">
        <property type="term" value="P:calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026208">
        <property type="entry name" value="Wolframin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045400">
        <property type="entry name" value="Wolframin_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026209">
        <property type="entry name" value="Wolframin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045461">
        <property type="entry name" value="Wolframin_OB_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20053">
        <property type="entry name" value="WC-rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19913">
        <property type="entry name" value="WCOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02060">
        <property type="entry name" value="WOLFFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02061">
        <property type="entry name" value="WOLFRAMIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="188"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Wolframin cysteine-rich" evidence="4">
        <location>
            <begin position="420"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Wolframin OB-fold" evidence="3">
        <location>
            <begin position="537"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="674" mass="76955" checksum="420C3B2641DA56C5" modified="2018-12-05" version="1">MTKKYAQGIAPSPFMASAADDDDDDDDVVVKNPDELPLHQKLLKFPLYALLEIKEHLIDWASRAGMQWLSALIPTHHVNALIFFFIISNLTIEFFIFLIPLLIFYLSFFSMIICTLRVFQNSKAWENFRALTDLLAHFEPGLDLDQAETNFGWTHLEPYLYFLLSVVFVVFSFPVADKSWVPSSELAAIALFFTITAFLSLHASAQLFARKALLSEVLSGACSLTQLLPDSFWFFRIFGKTFVTVPLGDAVVLNLGIPWLLYGHLFYLLFCMAQLKGFKGTYLCLVPYLVCFTWCELCLAFLNNASAIGLIRTCIGYFLFLFALPVLTLGLAAMIMIQLVQWFLTLEVTKMVVTLIVCFVPVVLRLWTRFSLNPIVVLRSLSRSSVVKLILVWLSAVVLFCWMYVYRSEGMKVYNSTLTWPEYSNLCGPLAWKESNMAQTQILCSHLEGHRVTWTGRFKYVRVTDIENGPQSTINLFPVFLGNWMRCLYGEPYPMCDEGKQNAAAEPQPLPAPAPSQEDPLCKLKTLAKHECHIKRFDRYKFEVTLGMPLERKTKDGTIIEDEDATKDIVLRASNEFKSVLLHLNAGSLVEFSTILEGRLGSKWPVFEMKAIHCLSCGDTRVPKRKQFKIEHDWRRTAQNALQFGFDFFFNPFLTARLEEQSDTETEMVAQEDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KU69</accession>
    <name>A0A3B5KU69_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD kinase 2, mitochondrial</fullName>
            <ecNumber evidence="6">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">NAD kinase domain-containing protein 1, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Mitochondrial NAD(+) kinase that phosphorylates NAD(+) to yield NADP(+). Can use both ATP or inorganic polyphosphate as the phosphoryl donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>NAD(+) + ATP = ADP + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KU69"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001525"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001550.1">
        <property type="protein sequence ID" value="ENSXCOP00000001525.1"/>
        <property type="gene ID" value="ENSXCOG00000001231.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006320"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012355">
        <property type="entry name" value="NADK2_mit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13158">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13158:SF5">
        <property type="entry name" value="NAD KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017565">
        <property type="entry name" value="Kin_ATP-NAD_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="4 6" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR017565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="401" mass="45081" checksum="00CDD2C8E4144017" modified="2018-12-05" version="1">MARCPVVNLLRFGNRVARSGFLRPLHAAAGCSSTEPRTGFKPDRVAVVTKTTRYEFEQQRYRYAGLSEEDLKQLLAMKGSSYSGLLERHNIHTSNVEHIVQSLQSAGMEVRVVRRGEYDEEVVRWADAIVSAGGDGTMLLVASKVLNKDKPVVGVNTDPERSEGHLCLPVRYTHAFPEALERLCRGEFRWLWRQRLRLHLEGTGINPTPVDLHEQQLSLEQHNRAHRISTVERQQTLHPAYSKPSLLPVRSLNEIFIGESLSSRASYYEISVDGGPWEKQKSSGLSVCTGTGSKAWSFNINKLAEQSVEEVLRIVTDDYNASLVFAPDEPRLFYSVREPISNHVFSSSRQRGFASRVCVRSRCWDACMVVDGGTSFEFNDGAIATVTVSEEDRLRTVLLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KU83</accession>
    <name>A0A3B5KU83_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ephrin RBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the ephrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KU83"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003678"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003717.1">
        <property type="protein sequence ID" value="ENSXCOP00000003678.1"/>
        <property type="gene ID" value="ENSXCOG00000002886.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155868"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046875">
        <property type="term" value="F:ephrin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048514">
        <property type="term" value="P:blood vessel morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031328">
        <property type="entry name" value="Ephrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019765">
        <property type="entry name" value="Ephrin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001799">
        <property type="entry name" value="Ephrin_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304">
        <property type="entry name" value="EPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304:SF18">
        <property type="entry name" value="EPHRIN-B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00812">
        <property type="entry name" value="Ephrin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01347">
        <property type="entry name" value="EPHRIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01299">
        <property type="entry name" value="EPHRIN_RBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51551">
        <property type="entry name" value="EPHRIN_RBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017363913" description="Ephrin RBD domain-containing protein" evidence="9">
        <location>
            <begin position="23"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Ephrin RBD" evidence="10">
        <location>
            <begin position="23"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="297" mass="33403" checksum="D54BF714C7798E9A" modified="2018-12-05" version="1">MGGRARSCGAVILLAIVCSCRGVTLESIHWSSSNSKFTPGQGLVLYPQIGDKMDIVCPRADPSSGRTEEFYKVYLPDQDVKFTFKFQEFSPNLWGLEFLKGKDYYITSTSTGSLRGLDNTKGGVCRTKSMKLVLRVGQSKQHAFILVFTNKDVHTHIHARAQSRICAVKRINGNSCFALKGYTGENQLISLSLLLVIVVVLVVLWRYYQRRRVPDRQRQQQHQPPASVSLSTLAVPKRDSFSSDNDGSDRSEVVFPLRPSESVICRHYERVSGEYGPPVYIVQEIMPQSPTNVYYKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KVR8</accession>
    <name>A0A3B5KVR8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PLD phosphodiesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phospholipase D family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KVR8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001706"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001731.1">
        <property type="protein sequence ID" value="ENSXCOP00000001706.1"/>
        <property type="gene ID" value="ENSXCOG00000001377.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183059"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050874">
        <property type="entry name" value="Diverse_PLD-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032803">
        <property type="entry name" value="PLDc_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10185:SF9">
        <property type="entry name" value="INACTIVE PHOSPHOLIPASE D5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10185">
        <property type="entry name" value="PHOSPHOLIPASE D - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13918">
        <property type="entry name" value="PLDc_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="3">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="285" mass="32246" checksum="D97AF016450AB9ED" modified="2018-12-05" version="1">KCEVDGRNEDEEQHRHTQQKCIATLALFCCSAVLMVLIFSSVDIWGDSEDGITEENCSRDCRIVLAENIPDDLSLHVDGRHHIPLSVGFQSLLEQAKLSVEVVSSVWDLNAWDLEPTPGTAAQGQLLFQRLLRLKSRGVKLKIASSLTNSSELKALAAHNAQVRFVNMTALTKGGLHSSFWIVDRTHIYIGSAEMDWRSLSKRKELGVVVYNCSCLALDLHRVFSFYWQLHDRDYIPSIWSKRVTALYGKHEALELQLNTTPATAYLSVRNALFYDQKLYCMLMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXX3</accession>
    <name>A0A3B5KXX3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Gap junction protein delta 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXX3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002481"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002515.1">
        <property type="protein sequence ID" value="ENSXCOP00000002481.1"/>
        <property type="gene ID" value="ENSXCOG00000001983.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286980"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086077">
        <property type="term" value="F:gap junction channel activity involved in AV node cell-bundle of His cell electrical coupling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF5">
        <property type="entry name" value="GAP JUNCTION DELTA-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0303">Gap junction</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="10">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="11">
        <location>
            <begin position="137"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="28103" checksum="6B40227466276484" modified="2018-12-05" version="1">MGEWGFLRSVFDNLQAHSPMLGRFWLFLMLMFRILILGTVASDLFEDEQLEFTCNTLQPGCKQVCYDKAFPISQYRFWVFHIVLIATPSIVFLLYATHQNKKKALRPKSRRSSIQNYQDDRRLRRLYIINVIFRIIAEVAFLVAQCKLYSFKVPAQYPCDRFPCPYTVDCFTSRPMEKTIFLYFYFGVGVLSAFSSIVELFYSSIKWFCCNQEDFDLEDRACKNLQNFRQDESGKKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KY94</accession>
    <name>A0A3B5KY94_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Phosphofurin acidic cluster sorting protein 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PACS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KY94"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005078"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005139.1">
        <property type="protein sequence ID" value="ENSXCOP00000005078.1"/>
        <property type="gene ID" value="ENSXCOG00000003980.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183209"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019381">
        <property type="entry name" value="PACS1/2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057541">
        <property type="entry name" value="PACS1/2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13280">
        <property type="entry name" value="PHOSPHOFURIN ACIDIC CLUSTER SORTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13280:SF15">
        <property type="entry name" value="PHOSPHOFURIN ACIDIC CLUSTER SORTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25332">
        <property type="entry name" value="C2_PACS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10254">
        <property type="entry name" value="Pacs-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="729"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="763"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofurin acidic cluster sorting protein 1/2 N-terminal C2" evidence="6">
        <location>
            <begin position="32"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofurin acidic cluster sorting protein 1/2 C-terminal" evidence="5">
        <location>
            <begin position="456"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="192"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="261"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="387"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="261"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="397"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="427"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="795" mass="89485" checksum="DB8DDC549CDA4B47" modified="2018-12-05" version="1">GGERSSFRLPGVPSNLPFCLNLHLSFCLFVSLSRLCSLTLKKLVVMKELDKELISVVIAVKIQGSKRILRSHEIVLPPSGSVETDLALTFSLQYPHFLKREGNKLQIMLQRRKRYKNRTILGYKTLAVGSIDMAEVMQHPTEGGQVLPLCNNQKDMLGKVAEIWIFSLSSQPIDHEDAGLQGGQKIKCTDNYSEEEYESFSSEQEASDDAVQGQDLEDDEFDVRKPKKQRRSIVRTPSITRQQNFKQRVVALLKRFRVSDEVLDSEQDPAEPPPEVEEEDLDLDSVEFENPSDSCPELDDDDSVLSTPKPKLKPYFEGLSLSSSQTEIGSIHSSRSHKEPPSTFTFDSQPEHLVGFIIFSVFLQEQPEAVTPTTELEMDNMDTFLERLPPSGKMTKTESLIISSNRQEAKLAGRRGRSTSLKERQPSRPQNERANSLDNERSLDTRCHLQIPRKTVYDQLNHILVSDNHLPDSIILINTSEWQGQYLSDLLQNHHLPVVCTCTTADIQAAFNTIVSRIQRFCNCNSQTPIPIKIAVAGAQHYLSAVLRLFVDHLSHKTPDWLGYMRFLIIPLGAHPVSKYLGTIDHRYNCLFQDAAWRDLFHKTEAPVIVQESPDVVSRVTQYMEGANGAHQLPIAEAMLTYKQKRFVFVSPQDFSLISSDCSRNVLDKIPPSLSLDPLLSLSLPACLCSLSVSSLGGRWQVSSRQEQQPYASSNHFTFRKRKSYNSAALHPAIFLGVAGKIALTVAGLCTTANRQCMDPDRFLDLGWCSSFFLLAMLFSHLMNLLPPECLPDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYH9</accession>
    <name>A0A3B5KYH9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. BTN/MOG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KYH9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003122.1">
        <property type="protein sequence ID" value="ENSXCOP00000003085.1"/>
        <property type="gene ID" value="ENSXCOG00000002448.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271914"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903037">
        <property type="term" value="P:regulation of leukocyte cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050863">
        <property type="term" value="P:regulation of T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000142">
        <property type="entry name" value="V-set domain-containing T-cell activation inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053896">
        <property type="entry name" value="BTN3A2-like_Ig-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050504">
        <property type="entry name" value="IgSF_BTN/MOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100:SF149">
        <property type="entry name" value="BG-LIKE ANTIGEN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100">
        <property type="entry name" value="BUTYROPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22705">
        <property type="entry name" value="C2-set_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017441370" description="Ig-like domain-containing protein" evidence="10">
        <location>
            <begin position="17"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="8"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="11">
        <location>
            <begin position="221"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="413" mass="45927" checksum="0014541C34980FB7" modified="2018-12-05" version="1">LSIVFVFVTFTVSVKSPVSVQRSQTATLPCWLTPSQSAEDMEVRWNHWNDEFDTPVLLYKGRTIDSSSQKASYVGRVSFGLKEETSGGLKAGDVSLKLENVTIEDAGKYICYVSSSEDYDSATISLSVTETGRSPLLSAVMKDDNKVNMSCQSDGWHPKPELSWSDRKKALIPSNVSFSKNSAGFYSVHSWVLVSSSSEVSCSVGLFNENIDKHMEGLKILWNNIVDIVHNLVIILYRLNQNIQLVDTGNLLIKMKDSTLRENIHAQFPDGDRVTCFTAVRGSPGFSSGRHYWEVSLQTENVDLKTSWWIGVTDKHEIPHDESFPPTTKNGFWFLSSFTKQGKTLQFSTEPETFIHVQSKLKTVGVYLDFDNRDLSFYNVEEKCLIGSLAAKFTGEVSSASRGTPVPGSPPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZA9</accession>
    <name>A0A3B5KZA9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">THUMP domain containing 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">THUMPD3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZA9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000899"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000911.1">
        <property type="protein sequence ID" value="ENSXCOP00000000899.1"/>
        <property type="gene ID" value="ENSXCOG00000000159.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063557"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016423">
        <property type="term" value="F:tRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11715">
        <property type="entry name" value="THUMP_AdoMetMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000073">
        <property type="entry name" value="THUMP domain containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053943">
        <property type="entry name" value="RlmKL-like_Mtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14911">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14911:SF13">
        <property type="entry name" value="TRNA (GUANINE(6)-N2)-METHYLTRANSFERASE THUMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01261">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="THUMP" evidence="9">
        <location>
            <begin position="163"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="140"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="476"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="478"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="498" mass="55117" checksum="8212443815780F4E" modified="2018-12-05" version="1">MSSQADEAAEQTSDTVEAPTSSSSTETDPTSGTIITVTVGATVPTGFEHTAAEEVKEKIGVDARISKDRGRIYFPITTDKLFQVHLLRSVDNLCVVVAEYDHYQFKESKEETLNELQELASKLPWTNALEVWRLNRTLKKKKGHRKAGNATRAKSNAEPSEVPAAAAAETQQHELPQVTSPDDDDAKAEVTPDSETCQQDLEDTAPEVKLIKFRVTCNRAGDKHSFSSNEAARDFGGAVQEFFQWKADMTKFDIEVLLNIHNDEVVIGIALTEESLHRRNISHFGPTTLRSTLCYGMLRLCKPQESDIILDPMCGTGAIPLEGAIEFSESFYIAGDNNDMAVNRTVNNICHIQKRRADKGSPSGLPIDTVQWDLSNLPIRTSSVDIIITDMPFGKRMGSRKKNWDLYPSCLREMARVCRPDSGKAVLLTQDKKCFAKAISRMGGLWRKLHTVWVNVGGLHAGVYLLKRTGTVFGQAPEDVHESRGRGDQPPAKETESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L0G2</accession>
    <name>A0A3B5L0G2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Msx2-interacting protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">SMART/HDAC1-associated repressor protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">SPEN homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM Spen family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000005818"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005884.1">
        <property type="protein sequence ID" value="ENSXCOP00000005818.1"/>
        <property type="gene ID" value="ENSXCOG00000004516.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157087"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12349">
        <property type="entry name" value="RRM2_SHARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12350">
        <property type="entry name" value="RRM3_SHARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21543">
        <property type="entry name" value="SPOC_SHARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000088">
        <property type="entry name" value="msx2-interacting protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000143">
        <property type="entry name" value="msx2-interacting protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.290.10:FF:000002">
        <property type="entry name" value="Spen family transcriptional repressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034173">
        <property type="entry name" value="SHARP_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034174">
        <property type="entry name" value="SHARP_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012921">
        <property type="entry name" value="SPOC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010912">
        <property type="entry name" value="SPOC_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07744">
        <property type="entry name" value="SPOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SPOC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50917">
        <property type="entry name" value="SPOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="8 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RRM" evidence="19">
        <location>
            <begin position="293"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="19">
        <location>
            <begin position="396"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="SPOC" evidence="20">
        <location>
            <begin position="2904"/>
            <end position="3070"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="93"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="142"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="648"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1118"/>
            <end position="1212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1269"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1385"/>
            <end position="1912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1938"/>
            <end position="2009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="2036"/>
            <end position="2102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="2147"/>
            <end position="2176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="2198"/>
            <end position="2218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="2261"/>
            <end position="2318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="2446"/>
            <end position="2485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="2552"/>
            <end position="2609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="2719"/>
            <end position="2746"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="1328"/>
            <end position="1355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
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            <begin position="208"/>
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        <location>
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            <begin position="1402"/>
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        <location>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50917"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005818.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    <sequence length="3070" mass="341311" checksum="1A8F2B5EA3A22C36" modified="2018-12-05" version="1">MVRETRHLWVGNLPEHVREEKIMEHFKRYVTQAGHRVCGAARAGCSTNLFSMRGYAANSSPSGSRDVAGFPRGTVGPVFGPPVSLHTREGRFERRIDGSSESRERAYDHSPYGHHERSGTFDRQRHYNADYYRDRPVFAPAGPGSSAIGGSFEAADPHFDSRIRDPFTLTNSTRRDLYRDDRGRRVDRSYHHRRSRSSHSSQSRHPSPQRTAGQTSKTPHSPKRTPLSPGRGPRSRSQSRSSSSDSVSSTSSTGSGSDSNSSSSDGSRARSVQSSAAPQTCLVMDSEEPRRSFGIKVQNLPVRSTDTSLKDGLFHEFKKYGKVNSVQIHGASEDRYGLVFFRQQEDQEKALSVSKGKLFFGMLIEVTAWNGPETENENDYRPLDGRLDEFHPKATRTLFIGNLEKTTSYQQLLDIFQRFGEIVDIDIKKVNGIPQYAFVQYSDIGSVCKAIKKMDAEYLGNNRLKVVILSPIKKEPFLFCVELFTVYVYKIVVFDRIKEMALILYNNTDFAQAAVRETKGWKIGGNKIKVDFASQESQNAFYRSMQASGQDIRDFYEVPPERREDRRPPYHEFTAERAYYENIRTTGLYPEEARREYAARSRERFSELEHYQGEHFDPRYHEDPRDYRDYRDPFEQDIRKYTYIQRERERERERFEADRSRWSPSQRRAVTPTISPSPSERAARDSERRVYSQSSERSGSVSSMSPPHLDKSEKTLLDHAAKSEKSAQPDRMAGAEKIKRPKRKDKTDKEKTEKNKSRKGKGQSPASQIPETEPDAAFDGGRGRGSDQDVHEKPKSKVDGDALSGNQLTASQDSVKTETTKGDIAELDGKPKLKKQPKSDIGNDGKDSAVDSDRLAARKRRFGDLGGKTVRQKRSRHEEEDGSQSSDFGSSTTYVKEPDADKHKDPQRRDARLKTEKSVTQKDGLEDLRGQREKSEGSADLPESKRQQGSTSSRRPSQNAETNKNAKTKEDHVDIDLSQSYRKQMEQNRRLHMQQQQQRESDKLKKAESLLKNETEDLEHRSLVHEVGKPPEDVTDNFPSHKLSDQFEAEPGIKRERFYRRQKSEDPDWSSTPSPGHQVFSQHADEDFMDASLKELSRNNQKIHPELELLVKRTHNTQVNKTNTPINIGEEDQQKRWESRGKQDLLPNLNFSRGLGKNLHNRKPSEYGLWHDLEPGEVRSDSEEDRENKPLSPAPSTSMPFSERPRPDRFSDPKLAHLERNKFYSFALDQTITPDTKALLERAKSLSSSREDNWSFLDYDSHFVNFRSQKDTEKVESAPRPTPSWYMKKKKIRSGSEDKLDDRKEEPKPEEQERRELFASRFLHSAVFELDSRRLQHLERKYEELEHMLNQQASQQGAEGELETGPVVLFHSRFLELTQLQQQKNKSQQLQEDKGNTVCTSENKEEKPSEQEQQDLRSNDMPESSVAAEIKPISPVEQVAPEPKLTPNLVIQSASKDTPSSHQKCVLPTPPADVCPPMSFIKEETKEEVKESEDVVPMHCSPTETLKSEPAPETVPETNISEENLEFLEEDAKPLIDEQTNDPGSHEELVSRSEPEPSPEMSQPEVPTASSPVQLSVAEEADLIKEDPLSTCQKTVESEGEKKLEEVSKEEISVDSDAIEEPVSFQKENKTKEIKTKKGRQSPAQVPLTSVSEKQATRKSERIDKDKLKRGSSPRAETKSSAKSPTPGSDHDHSEQSIPISRARTRRNVKSVYATPVEDDAPTRGKETESPRSARKRGTEKEALQHSTESEPPTPAPAKQRGRPPKNRKPIEEVSAAKTEKIKMDKDADSNESENGERVSRVAKGKMSPYLTKGSSNPIPSVPGSKKGDKTETADENQVIHFTEAEDLSSQHSSSSGKDSSVKSDVKKGSELDKDVHEKLCEEKSNGKETDSLLGEEKPAPEKEKIIKGKVKAAKPHVFRDLQVILDVKEVKDHVSRCTTANKECPNEEKEKKDVDSSKSSSLQERELEQAVENIAKLTEPALPTELPTPVPPAEVKSDPEEEKPCNPASETELMAAIDSITGEDVSVPLTPAAPVSTEVCSEPEVQEFVPPVKVEEPETALQEEAVFSNTPKKSYKGRPKTPKRPKAPKHARKDVKEELSEELTVPLAESTVSDVKIVAEKPPSAAAAAVITPTTWKSEAELPAVKAAESELPSPVEEQTQAVKPTHPQAKSPIYPQQLPAECVSLSLASNRPNIRSIQTSRAPVSPPDWRHQSKDPCVTSAHIMSVAAKEGQLTTSDSNSKELDQGTSDLRQILMKPKTITLPGGGSSAPTNLPSLRDQNPSESNPAITAALHNRSPLPESKTPTHSVPPIVRTPSTLPGETKSVISVIASTATSVISRVCNPPETEEKPNLIIGNPSMDVTLPKTAYRPSKDDGGPYHGATFSSGSCSGLRVNTSEGVVVLSHSGQKTEGPLRISAKISQIPQATAGDIESQQLVPIPQIKQDVYSHSQSGHQKGSSQTDHGHPGKLQSALSSIKQESGSCSLEKMDSAYQAGPQGVVKRLPQGNQQVMGYHQDYLQLKHQKKMENVDPHGTDGAKPSWTSTVSPAISPHLPSPPGNHVGFVTSTGDRGPSHLGSIKQEPRSPRKSGHSHPPFTKVSSPLGSSSPKGIPVMISSALNPMQQYITGVHHPEQSVIMPPHNVPGGVGRMSPHCVSQSITVGHLVQGEVRVNTPPLSVMNYVHSEKLASPWSGSLQPRSSSPQAVSRDKVLKVNHGSLRGHEGEQDESRHFHQAGRPSATLPTDTRGALRTNMETFIAKRDMRVHLHQQGERLTLDPHSGHIQETLPPSLSPRAHMLPKGVSEKDIKSLEAKRPHSPVIQKDGIMAIRQSGGAIASPQRVSLMPPGPSGSFSEYSGMYPNRMHSQIPDAPVGHNQPPLNVTPSMVSSLQLQAKPDGKMTQTVNMVQLLTKYPIVWQGLLALKNDTAAVQLHFVCGNKALAHRSLPLQEGGALLRIVQRMRLEASQLESVARRMTGDSDFCLLLALPCGRDQDDVINQTQALKAAFINYLQAKLAAGIINIPNPGSNQPAYVLQIFPPCEFSESHLSQLAPDLLNRISSISPHLMIVITSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1U2</accession>
    <name>A0A3B5L1U2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">WD repeat domain 25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">WDR25</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027857495.1">
        <property type="nucleotide sequence ID" value="XM_028001694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1U2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006268"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006343.1">
        <property type="protein sequence ID" value="ENSXCOP00000006268.1"/>
        <property type="gene ID" value="ENSXCOG00000004844.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114134841"/>
    <dbReference type="GeneTree" id="ENSGT00530000063583"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053053">
        <property type="entry name" value="WD_repeat_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44566">
        <property type="entry name" value="TRANSDUCIN/WD40 REPEAT-LIKE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44566:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="169"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="215"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="90"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="471" mass="51817" checksum="6520D0A04DC25660" modified="2018-12-05" version="1">MSSLVTYDDSEDEDESCDQKEEEQSDPSGFNSVNVKPRPAGSETVLGQSSSSLLYVYPKIQHQDWENRSGNEGKHFKNRSACLTFSKSIVDGSNPAKRRPSSLPGVRPYIPKRQRQSEETKCPENQPEQSLENPTRKGPVLSDVSPKVKPYLGEKPKSSGTPRKLLMSLEGHQGAVITAQWCPVPHVSHLLLSASMDKTFKVWDGAESGRCLRLYTCHSGAVRDACWTPCGRQLLTGSFDNTAAITDVETGQLTVKVDNQFKVMCVVLHPGSPDVFLCGGHSSVVKAWDSRCCKVVKTYKAAVQQTLDILFLRGGTDFITSSDCVSRDSADRTLIAWDYGSTAKISNQIYHERYTCPSLALHPVEESFVAQTNGNYVALFSSQTPFKMNKRRRFDGHKVEGYAVRCGFSLDGAILASGSSTGSAHFYDYQSTRALHAIQAHSQPCLCVSPHPVLPATAATCDWAGEIKIWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1Z5</accession>
    <name>A0A3B5L1Z5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Galactose-3-O-sulfotransferase 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the galactose-3-O-sulfotransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1Z5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004827"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004885.1">
        <property type="protein sequence ID" value="ENSXCOP00000004827.1"/>
        <property type="gene ID" value="ENSXCOG00000003786.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182923"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001733">
        <property type="term" value="F:galactosylceramide sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009729">
        <property type="entry name" value="Gal-3-0_sulfotransfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647">
        <property type="entry name" value="GALACTOSE-3-O-SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14647:SF57">
        <property type="entry name" value="GALACTOSE-3-O-SULFOTRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06990">
        <property type="entry name" value="Gal-3-0_sulfotr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="440"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="440"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="459"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="489" mass="56247" checksum="E7A65CDC6F8AF518" modified="2018-12-05" version="1">MFFRRGARMLRWLVCGRLGPVWMWKALLLFLAIAFAGQLLGVIFNKSNVQSAARSIFTSPDAQGPSLGSCQPHTHIMFLKTHKTASSTVLNMLYRFGDEHNLRFALPLGYQLGYPLPFNAHRVKGYRGPRAIEFHIMGNHMRFNKPEVEKVMPADTFYFSIIRDPVALAESSFAYYKEVAPAFRKAKGLGEFADDPKKFYDPRLRNNHYAHNLLWFDFGLDSNANFSLALARLGEDMIRRTFKLILVSEYFDQSMILLRHALCWPLDAVVFFSLNARQQKPISAGGTLPNLSLTPEQREKLRQWNAFDWYLYKAFNKTFWKEIDKFGHAQMEKEVRLLRMRREDLARVCLRDGGKPVEAHRIRDKSIRPFQSGLIKILGYELQPGLDNTTQMSCLRMIRPEIQYKDVLDSKQFPQFHAIPAQQQSQRLLVAVGGTFIKQEPPRKGIKETGGEAGAGGRTLEEGMKDWEGNRLVRNNQTLVRENGKGRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L223</accession>
    <name>A0A3B5L223_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L223"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001849"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001876.1">
        <property type="protein sequence ID" value="ENSXCOP00000001849.1"/>
        <property type="gene ID" value="ENSXCOG00000001482.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182979"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051145">
        <property type="term" value="P:smooth muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.30:FF:000002">
        <property type="entry name" value="Myocardin related transcription factor A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043451">
        <property type="entry name" value="Myocardin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004018">
        <property type="entry name" value="RPEL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22793:SF6">
        <property type="entry name" value="MYOCARDIN-RELATED TRANSCRIPTION FACTOR A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22793">
        <property type="entry name" value="MYOCARDIN-RELATED TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02755">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00707">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51073">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="RPEL" evidence="7">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="repeat" description="RPEL" evidence="7">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="SAP" evidence="10">
        <location>
            <begin position="272"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="94"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="222"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="577"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="454"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="228"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="597"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="740" mass="79678" checksum="F4EE860D251C31CB" modified="2018-12-05" version="1">MISPAAFHEQRRSLERARTEDYLKRKIRSRPERSELVRMHILEETSAEPSLQAKQLKLKRARLADDLNDKISHRPGPIELVHKNILSVKSVSCSVADSPKGAGGESSSLDEDSSDALSPDQLANHDSPLSAVTQLSPSHVLAQNGDMSPTQSLMAKVAHVPTSAPQVKKLKYHQYIPPDQKADKERPPQMDSSYAKLLQQQQLFLQLQILSQQQQHYNYHTILPPPSSSSGPSPSRTALTTTTTAPSAQTTAARQSQAAAAGAKPTTLPANLDEFKVAELKQELKLRGLTVSGTKNDLIERLRNYQEQNGNTAAALKNAAPQSVQQASASAATTNTTASASNTTPTFDHQLADGACFKLALSSLAQAVPGRVMRFGSTSSSPPVSPTPSERSLAGLSPDETSCNGDMFGEMVTSPLTQLTLHPSPQHPSNMEEIQSSCSMSKSSSKDPPAAGAIETLSLDKDQMLQEKDKQIEELTRMLRQKQRLVETLRSQLEQGKMVAGAVVVKKEGSEKSGTPAEAKLQTLIKASSAQLCRFLFFCVAVLRLCVCSQENGVNNQQMDDLFDILLKSGEIPGFKANPDPSLAPLHSDPPSPSIPSSPLHLSPPTLTDPFSSQLRAGEPRSGGGRLEDFLESTTGAPLLGMEPDGGGLTLIDDLHSQMLSTPSILDHPPSPMDTSDLSFSHHSAGLDFGDPTLDSMDWLDISMDGGTSLTPLVPHTPPSVFSADFLDSTDLQLHWESCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2I6</accession>
    <name>A0A3B5L2I6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">U3 small nucleolar RNA-associated protein 18 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">WD repeat-containing protein 50</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat UTP18 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2I6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001999"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002029.1">
        <property type="protein sequence ID" value="ENSXCOP00000001999.1"/>
        <property type="gene ID" value="ENSXCOG00000001602.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000033919"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000121">
        <property type="entry name" value="U3 small nucleolar RNA-associated protein 18 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045161">
        <property type="entry name" value="Utp18"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR18359:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 18 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18359">
        <property type="entry name" value="WD-REPEAT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="350"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="72"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="153"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="175"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00025767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058527"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00074442"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00075773"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="521" mass="58106" checksum="BC1EA2FCA7D97472" modified="2018-12-05" version="1">MEDENVIKLPVTENKITAKKRSRPPKFDPEEEEQRRQQNLKRLAVLGEEDGSVKRLETLVFGAEEELLERLVEEQTSGLQLEDEEGGGDEDSEGEEEPRQQPQPRKAAWVDEDDELEEEVDLKHRYRRNLMKGDAESAMSKQRLQQRMKEQFQKSMGGTPSWAQSSDQKKKKKKGESMSDDLLRRTGNFVAPSESLPSGVLRMKKCLHANSARPSADRLTTVQFHPSAQVVMTAGLDQSVSLFQVDGKTNPKIQSVHLERFPVHQARFSRDGRTVIATSLRNKMFYLYDMMEGRVAPVRSVRGLSEARVKDFSVCPDGGALLLTGTNGYLHMLTLKTNEIVRSMKINGDVSGVAFCPDGSKAFVNSEEGEVFVWDVRSSRCLSRFMDDGCVKGTAIAASPDGRYLACGSQAGVVNVYSQEACLNSTNPKPLRAVMNLLTSATSLTFNPTSEILAVASRVEDEAVRLVHLPSITVFSNFPVASRKVAHRVSCLDFSPRSGFFSLANNKGHAPLFRLLHYKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2U8</accession>
    <name>A0A3B5L2U8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome c oxidase subunit 7B, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cytochrome c oxidase polypeptide VIIb</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
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            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c oxidase VIIb family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2U8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005127"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005188.1">
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        <property type="gene ID" value="ENSXCOG00000004011.1"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="PANTHER" id="PTHR16716">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR16716:SF0">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
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            <begin position="68"/>
            <end position="87"/>
        </location>
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        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007351"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00040623"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00041642"/>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="87" mass="9598" checksum="E792ADA2E91F06DC" modified="2018-12-05" version="1">MYRFAKTVAILGGRAGRQLRHGSTAPQDFHSKYGMGVLVSGSVFCTAVWAYVLTQTGIVWNVSPVKRMTPKPWRDQPGEAEESQSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5E3</accession>
    <name>A0A3B5L5E3_9TELE</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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            <taxon>Atherinomorphae</taxon>
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            <taxon>Poeciliinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5E3"/>
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    <dbReference type="Ensembl" id="ENSXCOT00000003016.1">
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        <property type="gene ID" value="ENSXCOG00000002367.1"/>
    </dbReference>
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    </dbReference>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="100" mass="11204" checksum="99186E0DE7DF87AC" modified="2018-12-05" version="1">MVFRKLPGVSASGMGLRLSQKFVFLLFLSGLVTLCFGALFFLPDSVRLKRIFLSKTEVQPVTVGSGSENDVREHLKRPKEPEHTREQQKACIFCFGCPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7D6</accession>
    <name>A0A3B5L7D6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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        </lineage>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L7D6"/>
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    <dbReference type="Ensembl" id="ENSXCOT00000003739.1">
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        <property type="gene ID" value="ENSXCOG00000002900.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="118" mass="13806" checksum="07948A20CC140FE3" modified="2018-12-05" version="1">ISDTLMLLQRLPSQLTPYTILNISHFIFMIKCYGSSIMLWEYFSSAGTANLVRVDGKMKLNKGQSWRTLLGKQMITYQKENNSKHITRKIINWFRSRFILQPSIPRREGSCPLRPKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7M7</accession>
    <name>A0A3B5L7M7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Spermatid perinuclear RNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7X9</accession>
    <name>A0A3B5L7X9_9TELE</name>
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        <recommendedName>
            <fullName evidence="13">Phosvitin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="entry name" value="VWF_type-D"/>
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    </dbReference>
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    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0758">Storage protein</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017457650" description="Phosvitin" evidence="8">
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            <begin position="21"/>
            <end position="1601"/>
        </location>
    </feature>
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            <begin position="17"/>
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        </location>
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            <end position="1507"/>
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        </location>
    </feature>
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    </feature>
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        <location>
            <begin position="198"/>
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        </location>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1601" mass="177839" checksum="0230CDEC1B403CBF" modified="2018-12-05" version="1">MKVIVLALTVAFVAPEFAAGKTYEYKYEGYILGGLPEEGLARTGVKIQSKVLIGAANPDNYILKLEDPVISEYSGIWPKEVFHPATKLTSALSAQLLTPVKFEYANGVIGKVYAPVGVSTTVLNIIRGILNIFQTNIKKTQNVYYLQETGVKGVCKTYYVLSEDPKADRLHLTKTTDLNQCSEKINMNVGMAGYTDKCAECETRGEMFSGAMSLNYVMKPTASGSLILEATATELLQYSPFNIVNGAVQMEAKQNITFVDVKKTPLEPIKADYVPRGSLKYELGTELLQTPIQLLRITNAEAQIVETLNNLVSLNLGKAHEDSPLKFIELIQLLRVARYESIEALWRQFKSKSDHRHWMLSSIPAIGTHVALKFIKEKFVAGEVTAAEAAQAIMSSTHLVTADLESINLLEALAKTPKIQENAALREITMLGFGSMIHKYCAENPCTSELLRPIHDIITSALEKRDNDELSLALKVLGNVGHPSSLKPIMKLLPGFGSSASNLEHRVHVDAAMALRKIAKREPRMIQDVAIQLFMDSALDQELRIIAAIVLFDTKLPLVMVTTVAQSLLNEPDLQVASFVYSYMKAFTRTTTPDHSSVAAACNVAVRMLSPRLERLSYRYSRAFHYDHYYNPWMMGAAASAFYINDAATVLPRNVMAKARVYLSGVRIVKTKGIQTEGVQEALLKVRDVPGDADRITKMTQALKALIEWRANPSRQPLGSLYVKVLGQEVAFANIDKEMVEKIIEFATGPEIRTYGKKALESLLSGYSVKYSKPLLAIEVRHIFPSSLGLPMELSLYTAAVAAASVEVQATISPPPPENFHPVHLLRSDVSLKASVAPSVSLHTYAVMGVNNPFIQATVMSRAKVHTVIPKKMEARLDIEKGYFNYQILPIEGVKTIASARLETVAIARDVEDLVAAKVTPVVPLDNFSKKPILSLSQLLDSESNSEEFLPLKRQSQSVRNMKIPKPIVKKMCAATYTFGIQACIDIRSRNAIFLKKTPIYAIIGNHTLMVNVTPAAGPSIERIEIEVQIGEKAAEKILKAVILSEEDKILEDKNVLMKLKKILVPGLKNSTKSSSSSSSSSRSSSSSSRSSRSSSSSSRSSRSSSSSSSIKKEKTNTIYMSLCLQAKYLSNTVRPQITVLVRAIRQDNKNQGYQFAAYYDRQTARVQIIVANLTENDNWTICTDSVMLSYHKVMTRLTWGAECKQFNTTVTVETGSVDKKPAIRVKLAWTRLPHYMKKYSRRVSNWFYRVAKDNGVNRTKALNEPRQLKLTVAVVNETSLNVTLKTPKSNYYKLGWVTPLYLPMNNTAAEMQAYQGNWMEQVTYMLTKSNAAECAVVNDAVVTFNNRKFKTEMPHSCPQVLAQDCTNELKFIVLLKRDQTAEQNEISIKIENIDVDMYLKDKAVMVKVNGAEIPLNNLPYRHPSGRIHIKEKEEGISLFGPSHGLQEVFFSSDKVLVVDWMRGQTCGICGKADGEVRQEYVTPNERVSRNATSFAHSWVLPAKSCREASGCFMQLESVKLEKQVRVEGEESKCYSVEPVLRCLTGCQPVRTTSVTVGYHCVPMETNMNRSDGLSSIYEKSIDVRETAESHLACRCTPQCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7Y1</accession>
    <name>A0A3B5L7Y1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Coiled coil protein 74 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L7Y1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006932"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007020.1">
        <property type="protein sequence ID" value="ENSXCOP00000006932.1"/>
        <property type="gene ID" value="ENSXCOG00000005362.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00980000199751"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029422">
        <property type="entry name" value="CCDC74_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14917">
        <property type="entry name" value="CCDC74_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coiled coil protein 74 C-terminal" evidence="2">
        <location>
            <begin position="92"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="200" mass="22453" checksum="54572E2C20B7E75B" modified="2018-12-05" version="1">QESKSSTTSPTGIPGACSFSDCQRESATDGQIERRGSPNHIPPEERPISATTTNGNSREVSESPRSVRLNHGGELTGLRKTVLEPVRIDSTPPDMVHPSTMQECEAIIQQLFNVNSLQTQELCLCFQVFRLPKNRISSRNKEYFLSNKMYPLSQIYLSFPRPSRPGVVLPGLKQSLSSNAANRQKRLLPIHRHHFQQGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8B5</accession>
    <name>A0A3B5L8B5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Groucho/TLE N-terminal Q-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat Groucho/TLE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8B5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006695"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006777.1">
        <property type="protein sequence ID" value="ENSXCOP00000006695.1"/>
        <property type="gene ID" value="ENSXCOG00000005181.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234519"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000001">
        <property type="entry name" value="transducin-like enhancer protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005617">
        <property type="entry name" value="Groucho/TLE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009146">
        <property type="entry name" value="Groucho_enhance"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10814">
        <property type="entry name" value="TRANSDUCIN-LIKE ENHANCER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10814:SF29">
        <property type="entry name" value="TRANSDUCIN-LIKE ENHANCER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03920">
        <property type="entry name" value="TLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01850">
        <property type="entry name" value="GROUCHOFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Groucho/TLE N-terminal Q-rich" evidence="10">
        <location>
            <begin position="25"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="526"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="568"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="173"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="236"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="176"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="255"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="726" mass="77769" checksum="F4F8FB4DD9986000" modified="2018-12-05" version="1">SFPRREKMFPQGRHPTPHQAPGQPFKFTIPESLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMSLFISMCLLRHQTEIAKRLNTICAQVIPFLSQEHQQQVVQAVERAKQVTMAELNAVIGQQHLSHNHGGAPVPLTPHPAGLHPSQLGGSASLLALSGALGAIPPHLAGKEGSEKKPHLGGPDSHDSDGEKSDDNLVVDVSNEDAASPRGTPLPSPRENGLDKARLLKKDPCSPASTASSASSSSLKSKEMAMRDKAGTPGLKSSTPTPRGDSTPGPSSTPGVRPSLSKPPSMEIPHPPTAGLRTPLAVPGPYPGAFGMLPHTAGMNGELAGAASAAAYAAGLHNMSPQMSVAAAAAVAAYGRSPMVGFDPHPHMRVPGMPPSLTGIPGGKPAYSFHVAADGQMQPVPFPPDALVGPGIPRHARQINTLNHGEVVCAVTISNPTRHVYTGGKGCVKVWDISHPGNKSPVSQLDCLNRDNYIRSCRLLPDGRTLIVGGEASTLSIWDLATPTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVTKPDKYQLHLHESCVLSLQFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISVDDKYIVTGSGDKKATVYEVIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8D4</accession>
    <name>A0A3B5L8D4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM132 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8D4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006094.1">
        <property type="protein sequence ID" value="ENSXCOP00000006026.1"/>
        <property type="gene ID" value="ENSXCOG00000004669.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154702"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031437">
        <property type="entry name" value="Ig_TMEM132_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055423">
        <property type="entry name" value="Ig_TMEM132_5th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055424">
        <property type="entry name" value="Ig_TMEM132_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026307">
        <property type="entry name" value="TMEM132"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055421">
        <property type="entry name" value="TMEM132_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031436">
        <property type="entry name" value="TMEM132_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13388">
        <property type="entry name" value="DETONATOR, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13388:SF25">
        <property type="entry name" value="SI:DKEY-112M2.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16070">
        <property type="entry name" value="Ig_TMEM132_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23486">
        <property type="entry name" value="Ig_TMEM132_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23487">
        <property type="entry name" value="Ig_TMEM132_6th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23039">
        <property type="entry name" value="TMEM132_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15706">
        <property type="entry name" value="TMEM132_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="610"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 cohesin-like" evidence="10">
        <location>
            <begin position="81"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein family 132 fourth" evidence="9">
        <location>
            <begin position="136"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 fifth" evidence="11">
        <location>
            <begin position="237"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 sixth" evidence="12">
        <location>
            <begin position="383"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 C-terminal" evidence="8">
        <location>
            <begin position="589"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="560"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="696"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="521"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="569"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF15706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="781" mass="86434" checksum="B3A27FD416B52942" modified="2018-12-05" version="1">PELQLTFSTMLVIFLLNVSTFFSSIVFCFIHVYNFKDSLPPSHLIPLVQDYFMSFFALFPFLLAEFTLCLISVLHVFHRVEAVGYQRVLQVDLEVNGLMVTQGSREVTWQVEYPLTRTVTGEVQTLIRLAPQDLGGIVPLAMDTEILNTAVLNGRTVAVPVKVVTVGTDGAVSDVTESVECKSTDEQVIKVSERCDYVFVNGKETRGKRRVTLNFTYSFLSAQLEMSVWIPRLPLLIDVSDPELSQIKGWRVPVSAGSRRVTDDNDGVYGQEERTENACVAQYQSTSLHVLTQFVTDAGEFRGAMEEGRPGQQNFLLGTDWHVDVTQLVQESLRVQDPMIAELLESQVLIGLSSGTTKLQVLSPLTSSVLAERSIRVVDDKVSVTELGVQLVSGLSLSLQLSPGSNRAIVATASTQEVIESLTQESLISAWLQFSDGSMTPLDNYNPAHFILAATSLDEQVVVVQQSAKWKWPLIAAKGEGQGLLVRVEMSPSEVCQKSKRRSALATGMLNVQVRFGQSEEQYRQPGDRRTRPDPPYYGGSISDMDTGLINRAATTIRGQVPVRPNGGRLSSDSGNRNLNDFSEYPELPRSRSTDDDLLPSRRGLTDLEIGMYALLGVFCLAILVFLINCISYTYKYRSKHLSLEATETMPHAHDWVWLGQEEGLCLQQQQHEELTGTLEEGSQLLNGGIQRSITDGGGGSSSGRDLRNESLNSPTTKRKRVKFSTFSNVKSSNGCPVLSPLPLVQTSDIKWVCPDIELGDSKDLRNYMEKLNENAIKNTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8L4</accession>
    <name>A0A3B5L8L4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein 229B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM229 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8L4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008744.1">
        <property type="protein sequence ID" value="ENSXCOP00000008638.1"/>
        <property type="gene ID" value="ENSXCOG00000006606.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010899"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010540">
        <property type="entry name" value="CmpB_TMEM229"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31746">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 229 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31746:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 229B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06541">
        <property type="entry name" value="ABC_trans_CmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="19579" checksum="D60135A8D81AFFBB" modified="2018-12-05" version="1">MATTRTHPKGTLFRLYVYALHGCLCEVAFTAVYDWCSARDQRLPGHSSIWALPMYGSAIFCMEALRARLLAQSRSLPVRLAVYTLFIYMWEFSWGVGLSLMGACPWDYSAHRYNLRGLVTLDYAPAWAVAGLIAEQHVIKNTLKIRLKNVIIFGKRQISTNNFCQLPSVVLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L910</accession>
    <name>A0A3B5L910_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ras-associating domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSXCOT00000007391.1">
        <property type="protein sequence ID" value="ENSXCOP00000007297.1"/>
        <property type="gene ID" value="ENSXCOG00000005649.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051020">
        <property type="term" value="F:GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034446">
        <property type="term" value="P:substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="RA_Radil_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052072">
        <property type="entry name" value="Vascular_dev_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN YPR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027:SF3">
        <property type="entry name" value="RAS-ASSOCIATING AND DILUTE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-associating" evidence="2">
        <location>
            <begin position="42"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="157" mass="17476" checksum="BC7C519242FDF238" modified="2025-10-08" version="2">MSYRDRVPAETGTNTRGDRRSTTEPPERPADDPAELSTQSSAPGVLKIFGDEICAGANYKSVLATPCSSAQELVKEALERYSLNKDAARVYVLCDVIGRLEGGGAWRTECLRALGDNEKPLLLQELWKPREGHARRFELRRRAEVDELNAKEKDTVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9A5</accession>
    <name>A0A3B5L9A5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">3CxxC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027882609.1">
        <property type="nucleotide sequence ID" value="XM_028026808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9A5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004394"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004447.1">
        <property type="protein sequence ID" value="ENSXCOP00000004394.1"/>
        <property type="gene ID" value="ENSXCOG00000003469.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114150424"/>
    <dbReference type="KEGG" id="xco:114150424"/>
    <dbReference type="GeneTree" id="ENSGT00940000164175"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031849">
        <property type="term" value="F:olfactory receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051205">
        <property type="term" value="P:protein insertion into membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026096">
        <property type="entry name" value="R-trans_p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027377">
        <property type="entry name" value="ZAR1/RTP1-5-like_Znf-3CxxC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14402">
        <property type="entry name" value="RECEPTOR TRANSPORTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14402:SF8">
        <property type="entry name" value="RECEPTOR-TRANSPORTING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13695">
        <property type="entry name" value="Zn_ribbon_3CxxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01328">
        <property type="entry name" value="zf-3CxxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="3CxxC-type" evidence="8">
        <location>
            <begin position="47"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="160" mass="19019" checksum="4034E0FAD53F4DEA" modified="2018-12-05" version="1">MEEQEWIRIFQMEIETLNRKEHTWHLEFDENIDPDNPNMGWKKYFKKTSARFSCTSCRRSWPSNRVMVVFHMRLMNGRGTVKVRPLRQNCKTCSNAQMVKPSVESDNIQVLMETLMEKIRIKCYQEDLGEQNRRPRSLDVKSPHEPAHCEGCRLGICTKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9U8</accession>
    <name>A0A3B5L9U8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Solute carrier family 51 member A</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9U8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009112.1">
        <property type="protein sequence ID" value="ENSXCOP00000009003.1"/>
        <property type="gene ID" value="ENSXCOG00000006779.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163831"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005178">
        <property type="entry name" value="Ostalpha/TMEM184C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23423">
        <property type="entry name" value="ORGANIC SOLUTE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03619">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01417">
        <property type="entry name" value="Solute_trans_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="328" mass="36642" checksum="EB2E91D98AA239D9" modified="2018-12-05" version="1">MSNVSNRTANPCDGEPPFAIDNIRNQDIFGIILYSVLTFMATISLLVFLEECVYIYKKVPANKKSVIIWVNGAAPVISTMSCIGMWIPRAVMITDLTSATYFAIVVFKFLIMMLEEIGGDEAFLKRASKHKLKISTGPCCCCCPCLPHVAITGRTLFLLKLGSFQFALLKLIFTLLSIILWTDGNFNLQDMSITGAAIWINPFVGILTVIALWPVSIIFMHLRTTLRTLKIIPKYAMYQLVLILSQLQTAIINILAMSGIIACSPPFSSPARGYMLSQQLLILEMFIITLVTRLLYRRQYEPLPEEEPGDNENNKGMCASESLYAGVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAN1</accession>
    <name>A0A3B5LAN1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAN1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006791"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006874.1">
        <property type="protein sequence ID" value="ENSXCOP00000006791.1"/>
        <property type="gene ID" value="ENSXCOG00000005253.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168590"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF252">
        <property type="entry name" value="CYTOCHROME P450 26B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="298" mass="33783" checksum="3ACD0A47376C82BA" modified="2018-12-05" version="1">MCVFSAEPQCVYSIFKRLGAELVLSLFLNVRAEDQPEVFQEIMELCTEHWHGLISVPVNVKVPWWTSGFAKALDARSKLMEIIKDKLKNETQGFVGALGSVPFPNSGSAAQHLLLFISALIPKALASLLTSFTLALGGPEQRESRQEAIRNPDHLHRVLLEVQRLWPPFIGGRRIAEKESTLADFCVPKDYDVMYVSYFIHRDPEVFQQPDSFLPERWSGRNAGQEHLLCSFGNGPRSCIGSTLTEIFIKEACRYLLKNYDWCLDPPSQDLQYKWLPVSRPANLPSVSFTRLDQTVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LB18</accession>
    <name>A0A3B5LB18_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutathione S-transferase omega</fullName>
            <shortName evidence="7">GSTO</shortName>
            <ecNumber evidence="7">1.20.4.2</ecNumber>
            <ecNumber evidence="7">1.8.5.1</ecNumber>
            <ecNumber evidence="7">2.5.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Glutathione-dependent dehydroascorbate reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Monomethylarsonic acid reductase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Exhibits glutathione-dependent thiol transferase activity. Has high dehydroascorbate reductase activity and may contribute to the recycling of ascorbic acid. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-dehydroascorbate + 2 glutathione = glutathione disulfide + L-ascorbate</text>
            <dbReference type="Rhea" id="RHEA:24424"/>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="ChEBI" id="CHEBI:58539"/>
            <dbReference type="EC" id="1.8.5.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 7">
            <text>methylarsonate + 2 glutathione + H(+) = methylarsonous acid + glutathione disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:15969"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17826"/>
            <dbReference type="ChEBI" id="CHEBI:33409"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.20.4.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GST superfamily. Omega family.</text>
    </comment>
    <dbReference type="EC" id="1.20.4.2" evidence="7"/>
    <dbReference type="EC" id="1.8.5.1" evidence="7"/>
    <dbReference type="EC" id="2.5.1.18" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LB18"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008022"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008120.1">
        <property type="protein sequence ID" value="ENSXCOP00000008022.1"/>
        <property type="gene ID" value="ENSXCOG00000004189.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163896"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045174">
        <property type="term" value="F:glutathione dehydrogenase (ascorbate) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050610">
        <property type="term" value="F:methylarsonate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03184">
        <property type="entry name" value="GST_C_Omega"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000009">
        <property type="entry name" value="Glutathione S-transferase omega-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000123">
        <property type="entry name" value="Glutathione transferase o1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005442">
        <property type="entry name" value="GST_omega"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050983">
        <property type="entry name" value="GST_Omega/HSP26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045073">
        <property type="entry name" value="Omega/Tau-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43968:SF6">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE OMEGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01625">
        <property type="entry name" value="GSTRNSFRASEO"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01152">
        <property type="entry name" value="Main.3:_Omega-_and_Tau-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="8">
        <location>
            <begin position="33"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="9">
        <location>
            <begin position="117"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="252" mass="29374" checksum="5A46C6AA80A0657D" modified="2018-12-05" version="1">MLESRRFAENQSVIMCFCIKKKCHPAPGPVPKGQLHLYSMRFCPFAQRTRLALNSKGIKYDTININLKEKPEWFLEKNPLGLVPALETSAGEVIYESSITCEYLDEAYPEKKLLPSSPFGKAQQRMLLEHFSKVIPYFYKIPSARRTGEDVSTQEVELKEKFAKLNEHLEKKKTKFFEGDTITMIDYMMWPFFERIEVLELKHCLDHTPELKKWTERMLEDPAVKATMFNMDTHKAFYKSVAEGKPDYDLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LB33</accession>
    <name>A0A3B5LB33_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LB33"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007715"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007812.1">
        <property type="protein sequence ID" value="ENSXCOP00000007715.1"/>
        <property type="gene ID" value="ENSXCOG00000005931.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157944"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004028">
        <property type="term" value="F:3-chloroallyl aldehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000058">
        <property type="entry name" value="Aldehyde dehydrogenase 3 family, member A2a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF22">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="3">
        <location>
            <begin position="8"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="162" mass="18236" checksum="B4C13630987D148A" modified="2018-12-05" version="1">MSRRETQLLQRAKEAFVSGRARPLEFRLQQLHALQRMILEKETEISTALKQDINRSQYDTPLLELIGIENEIKLAIEKLAQWAAPRPVEKNLFTISDEVYIQPEPLGVVLIIGAWNYPWALTLIPLVGAIAAGNAAVVKPSELSECSSLLLHALLPRYLDKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LB95</accession>
    <name>A0A3B5LB95_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">One cut domain family member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CUT homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027863449.1">
        <property type="nucleotide sequence ID" value="XM_028007648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LB95"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007780"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007878.1">
        <property type="protein sequence ID" value="ENSXCOP00000007780.1"/>
        <property type="gene ID" value="ENSXCOG00000005973.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114138415"/>
    <dbReference type="KEGG" id="xco:114138415"/>
    <dbReference type="CTD" id="246225"/>
    <dbReference type="GeneTree" id="ENSGT00950000183103"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000054">
        <property type="entry name" value="One cut domain family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000005">
        <property type="entry name" value="One cut domain family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003350">
        <property type="entry name" value="CUT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051649">
        <property type="entry name" value="CUT_Homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14057:SF44">
        <property type="entry name" value="ONE CUT DOMAIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14057">
        <property type="entry name" value="TRANSCRIPTION FACTOR ONECUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02376">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01109">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51042">
        <property type="entry name" value="CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CUT" evidence="13">
        <location>
            <begin position="262"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="363"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="365"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="34"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="215"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="424"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="246"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="450" mass="49329" checksum="0AF3F8C83F087374" modified="2018-12-05" version="1">MDGGLGEMSLHSHSDLAHSQDGRAMLHTRDLSAAFPRPSLGSSSMSLEPEPRPSGYDHSMSALGYSGDSPSSSGSTYTTLTPLQPFDDKFHHHHHHHHPCLPVSNVIGSFTLMREDRGLSTNFYNPYGKELAMTQSLTPPTTGSGLGSSMHGYGSLGNSPNGNGGQMLHSGYDVHGGSLFCRASDFGREMSPPGLGGGEVSVGHQLNKIEAHQHTPNHHPHIYSQHYQPHHHPSQQSAKMGEHLHSSSPASSSPGEGMLPGSQGGGGGEEINTRDVAQRIITELKRYSIPQAIFAERVLCRSQGTLSDLLRNPKPWGKLKSGRETFKRMSRWLQEPEFQRMASLRLEACKRKEQEQSKLERNQGPKRTRLVFTDLQRRTLMAIFRENHRPTKELQVTISQQLGLELSTVSNFFMNARRRNLNKWADEGRPSSTGSSGSSVSSSAVSCSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBK6</accession>
    <name>A0A3B5LBK6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBK6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007895"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007993.1">
        <property type="protein sequence ID" value="ENSXCOP00000007895.1"/>
        <property type="gene ID" value="ENSXCOG00000006063.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154962"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12616">
        <property type="entry name" value="RRM1_TIAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12620">
        <property type="entry name" value="RRM3_TIAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000045">
        <property type="entry name" value="Nucleolysin tiar isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000087">
        <property type="entry name" value="Nucleolysin TIAR isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034492">
        <property type="entry name" value="TIAR_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034496">
        <property type="entry name" value="TIAR_RRM3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="8"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="104"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="212"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="377" mass="42125" checksum="457E22D763E581A0" modified="2018-12-05" version="1">MDDESYPKTLYVGNLSRDVTEILILQLFTQIGPCKSCKMITEVKILVWLLHTSNDPYCFVEFYERRDAAAALAAMNGRKILGKEVKVNWATTPSSQKKDTSNHYHVFVGDLNPEITTDDVRAAFSPFGAISDARVVKDITTGKSKGYGFVSFYNKLDAENAISHMAGQWLGGRQIRTNWATRKPPAPKSAQDNGSKQLRFDDVVNQSSPQNCTVYCGGIQSGLTEHLIRQTFSPFGQIMEVRVFPEKGYSFIRFSTHDSAAHAIVSVNGTVIEGQMVKCYWGKESPDMAKSSQQVDYSQWGQWSQQQQQQQQYGQYMANGWQVPSYNMYGQTWSQQGYGAEQSQSTAWMGGYASAPTPPDTVVSNVGNFNMAGYQMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LC41</accession>
    <name>A0A3B5LC41_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LC41"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007276"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007370.1">
        <property type="protein sequence ID" value="ENSXCOP00000007276.1"/>
        <property type="gene ID" value="ENSXCOG00000005630.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162658"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008514">
        <property type="term" value="F:organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000622">
        <property type="entry name" value="ATP-binding cassette sub-family G member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="390"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="422"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="465"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="497"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="534"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="616"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="31"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="640" mass="71770" checksum="C24C80103440F945" modified="2018-12-05" version="1">MTKGEQAVSDIETCFQEPGPTVTFTRLHYCVQEKTFCLKIGPEKHILKDVSGIMRPGMNAIMGATGSGKTSLLDVIAGRKDPAGLRQGTVRVNGKAVTSELRLSSAYVVQDDILMGTLSVRENLLFSANLRLNPKHYSSADKHERVNSILQDLGLVDCANTKIGTEFLRGVSGGERKRCSIGMELITSPSLLFLDEPTTGLDSNTANCITGLLHKLSRRGKTVIFSIHQPRYSIFKQFDHLTLMHKGEIVYAGAADHALEYFTDIGYQIEPFNNPADFFMDITNGEATCIKQSQTASNLFQTAKSYCAQSICNCEQENRQEGKNWLAMEYRESGLYQNVMQELDQMNESITDMDNRKEEAANYNTSFLYQMRVVCGRTVLNLLRNPQTSYAQLALNIFFAILVGLIYYQMPQTLPEGLQNRSGAFFFLIINMVFGNLSAVELFINERAIFIHENSSGYYRTSAYFLSKIFADLIPNRIIPIFVFSAIAYYMMGLNPAFVAFLCFALTMSLVSLAGVGLAFLVSASVSTFAMANILIALPFVFMMVFGGFLVNLNSMLSWLSWLKWISIFSYGLNAVYINEMKGQTFYSNNTIIPGEVFLKSMEIDYSVWGFWQNQVALLGIITVCMTLAYVQLLRINRWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCQ3</accession>
    <name>A0A3B5LCQ3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Solute carrier family 12 member 10, tandem duplicate 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCQ3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008733.1">
        <property type="protein sequence ID" value="ENSXCOP00000008627.1"/>
        <property type="gene ID" value="ENSXCOG00000006594.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155044"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008511">
        <property type="term" value="F:sodium:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055064">
        <property type="term" value="P:chloride ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055075">
        <property type="term" value="P:potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055078">
        <property type="term" value="P:sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018491">
        <property type="entry name" value="SLC12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002948">
        <property type="entry name" value="SLC12A3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004842">
        <property type="entry name" value="SLC12A_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827:SF97">
        <property type="entry name" value="SLC12A10.3 SOLUTE CARRIER FAMILY 12 (SODIUM_POTASSIUM_CHLORIDE TRANSPORTERS), MEMBER 10, TANDEM DUPLICATE 3 ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03522">
        <property type="entry name" value="SLC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01230">
        <property type="entry name" value="NACLTRNSPORT"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="328"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="495"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="520"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="559"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="7">
        <location>
            <begin position="130"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="8">
        <location>
            <begin position="634"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="13"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="861" mass="95877" checksum="56C179E895FFF9F5" modified="2018-12-05" version="1">ALMRQFASKRNVQTQGVHFSTNEEGPPRYSSHHFNGSTVNGGNGLQIPPTIVVSDESRLVPSLRVFSPSDIKSNRTIWLDNRRHGEILFFILNLMRVTEMLNMQHSLKKNNNKKITFLNWQLNYQHTHLVTVRCMLNIWGVILFLRLSWITSQAGVVLTCVIILMSVVVTSITALSISAIATNGRVKSGGAYFMISRTLGPEIGGPIGMVFSFANALACALNTVGFAEVVRDLMHEFGVVMVDSVYDVRIVGVITVTILLMISLAGMEWESKAQILFFLVLLVSFANYFVGTVIPPNVDKQAIGIFGYRGDIFVENLSSDWRGPQGSFFQMFAIFFPAAIGIMSGANISGDLKDPTIAIPKGTLMAIFWTTISYLGITVTVGSCVVRDASGNKSHILTGNNTDGCVGLACDLGWNFTDCSQSQTCQYGLANSVKVLGQVSGFYYLITAGVFAASLSSALGFLVSAPKIFQCLCKDQIYPYIIFFAKGYGKNNEPLRAYILCYLIAIAFILIAELNTIAPLISNFFLCSYCLINFSCFHASITNSPGWRPSFHYYSKWTALFGAVICVVLMFLFTWWAALVTWCIIIFLFGYVNYNKPSGCSSLNLSSVQLSDVELEASRKRFACLVLTGPPNQRPALVDFVCTFTKHICLMICGDIILVEQDRMTRPEDATDCLVKWLNKRKVRSFYTSLMADNLRAGAKQLLQASGLGKLKPNTLVLGFKTNWRDSAPESIEDYITIIHDTFDSNYGLCILRMMDGLDISDDLEFEGFLFSIFICLAWEKFFPDWGKPLQRSHYTPYDMKNDQINCIFQNKTKKSLNICHKNMNDLPTAISRYPSIQNMNIDCNKINSSLGLLRSGSSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LE51</accession>
    <name>A0A3B5LE51_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LE51"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009117"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009228.1">
        <property type="protein sequence ID" value="ENSXCOP00000009117.1"/>
        <property type="gene ID" value="ENSXCOG00000006954.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017498"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46731">
        <property type="entry name" value="F-BOX ONLY PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46731:SF1">
        <property type="entry name" value="F-BOX ONLY PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="347" mass="39701" checksum="635DC450F4841A6F" modified="2018-12-05" version="1">MNVQLLSNKTRDHVAGYWKELYCVTPQLLLLYFNIVLSSSRNLRVTWELTVTDMSGRESTLELSWSHFSETSLTLCWCGGDCLLNYEEIFSLQLHGIRRIAFSCPNLKKPSKRSLVANLDMQPLTKCAQVIGQDQFVLLKLLQPGIIIGLWRVIHSQAAVFFLHYYSLIIVAKAPIIQPPFDDIDPEYGLHGYKLHIVLHDTVCKLMSESFSQLFCHRAHISDGWIQLTAISRTNSSQHIQFSGKPMLPWRCEALEGTVENCCIMSLTLLDEFGNQFSCVTTPISMELEKAAVSYDYDGEHYLIQHEDSNIQVKMQLVKTTAKKHFVVSLIVYVSVLFVNKHFSRDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEC7</accession>
    <name>A0A3B5LEC7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LEC7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010636.1">
        <property type="protein sequence ID" value="ENSXCOP00000010513.1"/>
        <property type="gene ID" value="ENSXCOG00000007945.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286934"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000703">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003288">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003287">
        <property type="entry name" value="Zgc:113343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000464">
        <property type="entry name" value="Zinc finger and SCAN domain containing 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001506">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="110"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="138"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
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        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
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        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010513.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="280" mass="31750" checksum="64F16C642E93D399" modified="2018-12-05" version="1">MSEPEPVEIKQEREDLCGNQDEEQPNVFEFCGNSFMQHNELASHMIPQADEKPFSCLTCGRGFNKSNDLARHIRTHTGEKPYSCEVCGKSLTDSSHLIKHMRTHTPEKPFPCLTCGKGFTAQSDLTVHMRTHTGEKPFTCLVCGKGFIQKTTLSYHMRTHTGEKPFPCDLCDKSFIQSSDLARHLRTHTGEKPYSCDLCDKSFRQSSDLARHIRTHTGEKPFSCQICGKGFTKQNNLTVHMRIHTGEKPYSCEACGESFTVKFSLTKHKCKNANQEVLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LH60</accession>
    <name>A0A3B5LH60_9TELE</name>
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        <name type="scientific">Xiphophorus couchianus</name>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011468.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="268" mass="30468" checksum="9495A71F789D0F09" modified="2018-12-05" version="1">MNTSPAELQERYNKEGFLSPLPVLDETELREAREAFAKLEEEFGTEYTQYSLHNVHLQYPWVMNLTKHPRILQVIQAILGPDVILLDSRFICKYPTVKPAKLQESKTDQSKPDGDDELPYVAWHQDMRYWGIAGGPVLSVWLAFDDSQKENGVLQVIPGSHCSGMLLHRQATRPGNLLSVNQEIPEELVQVDEAVLCPLKAGQMSIHDGLLVHASDANTSQRRRCGFVIRYVPTYAYPTEDPDRPRKFHATELICGVDQFHHFSNKST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LH94</accession>
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        <submittedName>
            <fullName evidence="3">Si:ch73-132f6.5</fullName>
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        <name type="scientific">Xiphophorus couchianus</name>
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        <lineage>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <property type="term" value="F:phosphatidylcholine-retinol O-acyltransferase activity"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="domain" description="Acyclic terpene utilisation N-terminal" evidence="1">
        <location>
            <begin position="48"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="AtuA-like ferredoxin-fold" evidence="2">
        <location>
            <begin position="535"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
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            <dbReference type="Pfam" id="PF07287"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF23544"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="599" mass="64412" checksum="F0890EB700BACF6C" modified="2018-12-05" version="1">TGPLPHSPCGGSAEKCVSLSPRAKFQSPGVAHPDGRGGSRWYSSCKPVRIGCASGFWGDTATSAPQLIYGGKLDFLVFDYLSEITMSLLTAARTKTPNLGYAPDFVQVALAPFINDIHRKGIRVVSNAGGVNPLACAEAIQEVIKKAGLDLKVAVVTGDDLMPSRSLLSEVKMADGGGRRPLPKTLHSMNAYLGAEPIRRCLDLGADVVVTGRCVDSAVALGPLLHTFGWKRDDYDLLAAGSLAGHLIECGAQSTGGIFTDWHQVPDWDNMGFPVVECSSDGSFVLSKPPKTGGLVSFGTVAEQLVYEIGDPLRYLLPDVICDFSQVAIQELPGVDGGAVRVTGARGLAPSPDYKVCATYMDGFRATAVCPVGGPRAAEKARRTADSIIKRTSRMFQQLGLEDFNSVNIQVLGAEDTYGPNARRKDCREAVIWMAVHHQQKKALEIFSREVAPAGTGMAPGLTGIVGGRPRVSPVLKPFFFLHPKSQIKVDIHVAGELVESLAEVGPDSPEQFLGQTRRSFWFLSDLPSGPHTYRLEDLAFTRSGDKGDSANIGVIARHPLYFPYLKKHLTSSVVEDYFSHLIEGLYHVTLSNAGKHRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIK5</accession>
    <name>A0A3B5LIK5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="32473.ENSXCOP00000007704"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007801.1">
        <property type="protein sequence ID" value="ENSXCOP00000007704.1"/>
        <property type="gene ID" value="ENSXCOG00000005924.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160141"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich repeat-containing 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000184">
        <property type="entry name" value="leucine-rich repeat-containing protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050467">
        <property type="entry name" value="LRFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842:SF25">
        <property type="entry name" value="CARBOXYPEPTIDASE N SUBUNIT 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842">
        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017291599" description="Ig-like domain-containing protein" evidence="7">
        <location>
            <begin position="22"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="242"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="461"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="463"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="490" mass="53814" checksum="C8987792C024F200" modified="2018-12-05" version="1">MAVGFVLLLSLLLPSPRPSLGVSPPCPAGCRCYSLTVECGSTDLRDIPRLIPPSTQVVPFSTCHRLLFISSVEPGSFRDQSQLLELALNGNRIHLLTPNMLQGLEQLRILYLSGNDITRLLDYTFQGLQRLQELHLQQNSIEMIADKALFGLTSLALLDLSRNNLHTIGPTSLQPLVSLQVLRITDNPWRCDCALHWLRRWIDEDGQRLLSSAERRLVCNEPPRLSHLSLVEVPLNSLVCIPPVVQLEPRRLAVHLGESLRVSCHASGYPRPQVTWKKASQSQVVDSPRGLVQDLGTAGEYFDPDTGSGMLFLSNVTVAHAGFYECEAWNAGGVARVTFQLAINSSTSSSIWASCPVLGSDVSREPLYALGSMAFSALGAATQTAIAVGISLLALTALLLVAMIYSRHHQRDKDADGPTGESILYVNDYSDGPTTFAQLEEYRDEQGHEMYVLNRAKLPLLTRSGTPDSPRTDPNNPEQDKAHNLKTVQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIS8</accession>
    <name>A0A3B5LIS8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Fem-1 homolog A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of a CRL2 E3 ubiquitin-protein ligase complex, also named ECS (Elongin BC-CUL2/5-SOCS-box protein) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the fem-1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIS8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010782"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010907.1">
        <property type="protein sequence ID" value="ENSXCOP00000010782.1"/>
        <property type="gene ID" value="ENSXCOG00000008137.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161210"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000104">
        <property type="entry name" value="Fem-1 homolog c (C.elegans)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000133">
        <property type="entry name" value="protein fem-1 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173">
        <property type="entry name" value="ANKYRIN REPEAT CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173:SF12">
        <property type="entry name" value="PROTEIN FEM-1 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13857">
        <property type="entry name" value="Ank_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="82"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="115"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="148"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="181"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="482"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="528"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="617" mass="68332" checksum="6C94284C625874B1" modified="2018-12-05" version="1">MDITVAVFNAARDGKLKLIQKLLSNKTPEELEALAEEKTQGSTALLIASRYGHLEVVDYLLEHCKANVELGGSVNFDGETIEGAPPLWAASAAGHLPVVKTLLKHGASVNNATLTNSTPLRAACFDGHLEIVRYLVEHRADLEVANRHGHTCLMISCYKGHKEIAKFLLDRGADVNRKSVKGNTALHDCAESGSLDIMKMLLKCNARMERDGYGMTPLLAASVTGHTNIVEYLAHQPRTSREERINALELLGATFVDKKRDLLGAMRYWRRAMELREAGDKAGSLSKPPPGPPVPAYGCAQEVSTAEELEALITDPDEMRMQALLVRERILGPSHPDTSYYIRYRGAVYADSGNFERCISLWKYALDMQQSNLDPLSPMTASSFLSFAELFSFVLQDRAKGTLSTRITFQDLMTVLGKSVREVERAVAQRDNPPETPQFNKALSIILHLIFLLEKLECNPEQEHQKKHTVYRLLKLNPRGRNGFTPLHMAVDKETTSVGRYPVGRFPSQAVAALLLECGADVDSRDCENNTPLHVAANNGCPEIMALLVKAGAHFDATNAQRKTAYELLDDHSGGHTALYPLNYITLQCLAARAIEKHRLPYRGLISEEMEAFIELH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIW5</accession>
    <name>A0A3B5LIW5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Tyrosine-protein phosphatase non-receptor type 23</fullName>
            <ecNumber evidence="6">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="primary">PTPN23</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Plays a role in sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs) via its interaction with the ESCRT-I complex (endosomal sorting complex required for transport I), and possibly also other ESCRT complexes. May act as a negative regulator of Ras-mediated mitogenic activity. Plays a role in ciliogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007814"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007912.1">
        <property type="protein sequence ID" value="ENSXCOP00000007814.1"/>
        <property type="gene ID" value="ENSXCOG00000005995.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157687"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045022">
        <property type="term" value="P:early endosome to late endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09239">
        <property type="entry name" value="BRO1_HD-PTP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14539">
        <property type="entry name" value="PTP-N23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09234">
        <property type="entry name" value="V_HD-PTP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.280:FF:000002">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000061">
        <property type="entry name" value="tyrosine-protein phosphatase non-receptor type 23 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.560">
        <property type="entry name" value="alix/aip1 in complex with the ypdl late domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.50">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025304">
        <property type="entry name" value="ALIX_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016103">
        <property type="entry name" value="ProQ/FinO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF30">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13949">
        <property type="entry name" value="ALIX_LYPXL_bnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00945">
        <property type="entry name" value="ProQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="22" id="KW-0966">Cell projection</keyword>
    <keyword evidence="19" id="KW-0969">Cilium</keyword>
    <keyword evidence="13" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="18 26" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="23" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="20" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0488">Methylation</keyword>
    <keyword evidence="21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-0653">Protein transport</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="BRO1" evidence="30">
        <location>
            <begin position="8"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="28">
        <location>
            <begin position="1394"/>
            <end position="1655"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="29">
        <location>
            <begin position="1568"/>
            <end position="1646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="711"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="943"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1205"/>
            <end position="1229"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1254"/>
            <end position="1350"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1706"/>
            <end position="1725"/>
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    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1734"/>
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    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1810"/>
            <end position="1860"/>
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    <feature type="coiled-coil region" evidence="26">
        <location>
            <begin position="558"/>
            <end position="585"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
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            <begin position="1008"/>
            <end position="1019"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="27">
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            <begin position="1269"/>
            <end position="1285"/>
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        <location>
            <begin position="1300"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="27">
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            <begin position="1739"/>
            <end position="1760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
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            <begin position="1777"/>
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            <begin position="1846"/>
            <end position="1860"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00004120"/>
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    </evidence>
    <evidence type="ECO:0000256" key="26">
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            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
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            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    </evidence>
    <sequence length="1860" mass="203427" checksum="A2212F51E72B2FC2" modified="2018-12-05" version="1">MEAVPRMPMIWLDLKEAGEFQFSPSVKQFILKNYGENPDNYNEQLKKLETLRQNAVNVTRDFEGCSTLRKYFGQLHYLQSRVPLGGGQEAAVPISWTEIFSGKTVTHNDISYEQACILYNLGALHSMLGAMDNRVSEEGMKVSCTHFQCSAGAFSYLRDHFNHNYSVDMSHQILNLNINLMLGQAQECLLEKSMLDNRKSFLVARISAQVVDYYKEACRALENSETASMLGKIQKDWKKLVQMKIYYFAAIAHLHMGKQAEEQQKYGERLAYLQSSLDKLNEAIKLAKGQPDSVQEALKFTMDIIGGKFNSAKKDNDFIYHETVPSLETLASVKGAPLVKALPVNPTDPSVTGPDLFAKLVPMAAHEASSLYSEEKAKLLRDIMAKIDSKTETLEQFMDSLGLEPESVDNLDMYNHIPPVLMEKCAALSVRPDTVKSLIQSMQVLSGVFTDVESSLKEIRDVLEGDEAGERALQEMCGPTAAELHSAAQSQALAEIQRDLEKYMEAHEKASFTNTELHRAMNLHISNLRLLGGPLESLRDALPKPQLSEEEMAGLQRMKRILGKVQEMREQRGSLEKQLRDLIQTDDITSTLVTTERADMKHIFEDQLKKYEQVKVYIDQNLSAQENILKALTEANVQYASVRKGLNQTEQQWNSTVQGLVGSYEAYEDLMKKSQEGKEFYDDLEAKASRLLERAKTLCHTRAEERKFALHCRDKKPPARPTAAKPSLKPRTSDVDSGSSSLEDPELAQLNAAIMALGGDLPEDLRSLPPDISSLPTTAGRLPGPCSSSLPWAGGHAAGIPHFPPNLPPPELLARIAQFPTSGGLPTQGPLPCVPLPQMTHHRPPPMPAQPVVSGYRAPPPQAPQPGLVAPVPIRPSTTTVDSIQAPIPSYTPTPHHPVPPPASSGYTVPQIGAYPHMMNHPGAPIQVPGQTAAPQKHTYPQPIAQPLPQGYQPGPRAAPIPTQQVYTPGYIPPQPNVPPNYQQAFPGQLQHQQNGFPPRPQVPQGYQAPQNFIPQQHPQMMPGSMPRPPQTAHPPTSSTSHPTPPLSAPYSPYPNQQMPPGFPHQQMSQHQQIPLPPGAQHVLPHPQMQIPGGHRAQLPPTSQTIPPVSHNYIPPNLHPQMPPSSQPHVPSGPQVNLPRGPQMPQNALAAQHQPIPMQMPRQPHPHPSHPGGGCYPGGALMMPQQPLAPQPASLQQPQVSLPMTHSQPATLYPSPAGTNVPPGAPQPPSVVGPLSPAVPHMISPCPGGPAVVPPTNTVAPSPSPSLSPGPASLGMTPTPGGTAPPTLPTPSGVPPSTSLFQRQNSSTDDLLSSSPESQHGGTKGPTNVLQPTKADPQDGERRKKNSQGVLLIQGDPYQAPERVARLHSELGHYRAQVESLEHPSENEGGVSVLDARWKELQDQQEKDARQLSIAIARCYTMKNRHQDVMPYDFNRVVLQSGKDDYINASFVDDLSAYCPRLIATQAPLTGTAADFWLMVYEQKVSLIVMLVSEQELDKGKVLRYFPTERGQQLSQGPITLSLTTQKMTPTHVERMISLQYRDQSLKRTVVHLQFTSWPELGLPDSKSNLLRFIQEVHGHYLHQRPLQTPVVVHCSSGVGRTGVFCLLYAALQELEAGNGIPDLPLLVKKMRQQRKNMLQEKLHLKFCYEAVLKHAEQVLQRHGITTPYSRQESQQDIVLGGDMPISSIQATIAKLSIRPPSADLTMEGGASCGPEEHFGVMSPTLDSMGDVQLLQEPSQPPSSLSPPLTSPPHSPPPPNGLDCLSPSTPPAPNHQPLPESAPSASPPLASSVPAPSSLELLASLTPEAFSLEGGGRGKQRVTKQNFLQPAEGQGIHGTRGDDSSDPLSSLDPLWSLNKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LL97</accession>
    <name>A0A3B5LL97_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">3-hydroxyanthranilate 3,4-dioxygenase</fullName>
            <ecNumber evidence="10">1.13.11.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">3-hydroxyanthranilate oxygenase</fullName>
            <shortName evidence="10">3-HAO</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">3-hydroxyanthranilic acid dioxygenase</fullName>
            <shortName evidence="10">HAD</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">HAAO</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 10">Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>3-hydroxyanthranilate + O2 = (2Z,4Z)-2-amino-3-carboxymuconate 6-semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:17953"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:36559"/>
            <dbReference type="ChEBI" id="CHEBI:77612"/>
            <dbReference type="EC" id="1.13.11.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the 3-HAO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.6" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LL97"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008644"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008750.1">
        <property type="protein sequence ID" value="ENSXCOP00000008644.1"/>
        <property type="gene ID" value="ENSXCOG00000006608.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013008"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00330"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000334">
        <property type="term" value="F:3-hydroxyanthranilate 3,4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:L-tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06123">
        <property type="entry name" value="cupin_HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000077">
        <property type="entry name" value="3-hydroxyanthranilate 3,4-dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00825">
        <property type="entry name" value="3_HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010329">
        <property type="entry name" value="3hydroanth_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03037">
        <property type="entry name" value="anthran_nbaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15497">
        <property type="entry name" value="3-HYDROXYANTHRANILATE 3,4-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15497:SF1">
        <property type="entry name" value="3-HYDROXYANTHRANILATE 3,4-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06052">
        <property type="entry name" value="3-HAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="8 10" id="KW-0408">Iron</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 10" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Domain A (catalytic)" evidence="10">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Domain B" evidence="10">
        <location>
            <begin position="164"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>O2</name>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="274" mass="31924" checksum="76805A21F7E67FAC" modified="2018-12-05" version="1">MSSQVPLVNVEKWIAENQDAFLPPVCNKLMHFSQLLVMFVGGPNTRKDYHIEEGEELFFQLKGDMCLKVIENGLHKDVHIKEGEMFLLPARIPHSPQRKANTIGLVVERRRLLTETDCLRYYVDNTTDILFERWFYCENLGTQLVPIIKEFMASNQAKTGKPDPNEVFREPPFQMNTMNVMTPFSFKDWLNKQRPSLANGRPINIFGAQFETEVMVFGPGLTETSVQQTDLWIWQMAQILVQTYNSRFLCRYMWQRNEGTVALVVVQNPERKRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLT6</accession>
    <name>A0A3B5LLT6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Hydroxysteroid (20-beta) dehydrogenase 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLT6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008829"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008935.1">
        <property type="protein sequence ID" value="ENSXCOP00000008829.1"/>
        <property type="gene ID" value="ENSXCOG00000006730.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163913"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05356">
        <property type="entry name" value="17beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000137">
        <property type="entry name" value="Hydroxysteroid (17-beta) dehydrogenase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051019">
        <property type="entry name" value="VLCFA-Steroid_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899:SF10">
        <property type="entry name" value="20BETA-HYDROXYSTEROID DEHYDROGENASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899">
        <property type="entry name" value="RH59310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="293" mass="32567" checksum="817297728AA306E8" modified="2018-12-05" version="1">MLLGLEFSRKTLLTDVLALFGLFILVLCLLKFTWKCCCGFRQYVLSSLCQADLRAYGQWAVVTGASSGIGKAYATELARRGLDVVLIGRSSKRLQKVAEEIENKYGQQTHTIKVDFTEGGSIYSTIAKELHDLQIGILVNNVGMICNDHFAYFLETSIRGQKITEIINCNILSVPQMTRLVLPPMVLRGRGLIINISSEMGLRPHPLVALYSATKTFSIHFSQCLHAEYKSVGITVQVTCQYASEKTFVDIFHANSSTGEKGRPNRRLLPIAVYVNLRTPTASPRLAKPDLLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMA4</accession>
    <name>A0A3B5LMA4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Proline rich membrane anchor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PRIMA1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMA4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011850"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011986.1">
        <property type="protein sequence ID" value="ENSXCOP00000011850.1"/>
        <property type="gene ID" value="ENSXCOG00000008966.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006240"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029659">
        <property type="entry name" value="PRIMA1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16101">
        <property type="entry name" value="PRIMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017462691" evidence="2">
        <location>
            <begin position="25"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="158" mass="17660" checksum="7A90961A82A88905" modified="2018-12-05" version="1">MLLRDLMPFTLSFWPFFIAHCLLSQLLLSCKVSIGEIQRSCSWTVAEKVGEQCQVACHCRRYPPLPPPPPPPPPPRLLGTTVAQPMVPLLRPWWTEVDVIVLGTVSCASVVFLLSAIIICYKAIKRKPLRKEENGTSRGEYAMSIRNKKAMGTNNTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMG2</accession>
    <name>A0A3B5LMG2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Hexosyltransferase</fullName>
            <ecNumber evidence="16">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl derivative + UDP-N-acetyl-alpha-D-glucosamine = an N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl derivative + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14389"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:133507"/>
            <dbReference type="ChEBI" id="CHEBI:134090"/>
            <dbReference type="EC" id="2.4.1.149"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with B3GNT8; this interaction greatly increases B3GNT2 catalytic activity, independently of B3GNT8 enzymatic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Golgi apparatus membrane</location>
            <topology evidence="2 16">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMG2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013253"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013417.1">
        <property type="protein sequence ID" value="ENSXCOP00000013253.1"/>
        <property type="gene ID" value="ENSXCOG00000010051.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164878"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008532">
        <property type="term" value="F:N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030311">
        <property type="term" value="P:poly-N-acetyllactosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000010">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF234">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 16" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="444" mass="50858" checksum="3215D7D5CE01AC2D" modified="2018-12-05" version="1">MGKCCKCNGRLLCMCLLPCMMTGHLVIYIMVTIFVTISYAPPKVIIHYIAPGISANSSTLASHPLGPFWNLRLEDSALWNQLQHSWDRQHNPILRGNLTGMQRSPKGQPFTEIEDNCLSDCMSQKCSVPSVNDFNSMPEQIRAFIKSMHCRKYPLLINQPRMCQRNKDTTGVGSPMLLIAIKSQVGNFENRQAIRETWGHGGLVKGEFNTKGGLVRTVFLLGRQDSSTGPYPDLKNLLELENEKYGDILQWDFRDTFFNLTLKDLLLWRWFKQYCPTAAFVFKGDDDVFVRTDALLSYLHKKMEEHKLWRTYTNETDMNFFVGDVISNAMPNREPSMKYYIPESFYKCVYPPYAGGGGVVYSGSLALRLQEVSERVRLFPIDDVYLGMCLNRLGLSPVHHPGFLTFDLPATDRNNPCAYRSVLLVHRRNPKEMLTLWKQLQNLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMM1</accession>
    <name>A0A3B5LMM1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IGBP1/TAP42 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMM1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013318"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013482.1">
        <property type="protein sequence ID" value="ENSXCOP00000013318.1"/>
        <property type="gene ID" value="ENSXCOG00000010099.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002414"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051721">
        <property type="term" value="F:protein phosphatase 2A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035303">
        <property type="term" value="P:regulation of dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.540:FF:000003">
        <property type="entry name" value="Immunoglobulin (CD79A)-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.540">
        <property type="entry name" value="TAP42-like family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038511">
        <property type="entry name" value="TAP42/TAP46-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007304">
        <property type="entry name" value="TAP46-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933:SF9">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04177">
        <property type="entry name" value="TAP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="289"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="309"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="352" mass="40294" checksum="4EEBD496645CA656" modified="2018-12-05" version="1">MAESMDNAAEQSSAPDTEQLKLSDLLERGWKILQELDSSNEPFGPNTVHRIKRGINMLEDASRMVVQLDLFSRNEEVEEIATADLKYLLLPALLGALHMKQSSPHKRLETVQIARAYFMDFLKTCHDYNISQFELPKPSNENGVSQETSEGSFSAGPSNLVAMAAKRQTKIERYRQRKELETRLSDVHKVVESGQADDEVSREFYLLNVQRWITVCLEEMEAIDQEVEILKNMDLLKQSRANQPAQPPRNPMKPFILTRDALQAQVLGAGYPSLPTMTVDDWYEQHRKHGVLPDQGMPSKVAVEDNTDEQARDEEKEKKAENDDEASLLKARNWDEWKDDHRRGYGNRHNMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMN2</accession>
    <name>A0A3B5LMN2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ubiquitin carboxyl-terminal hydrolase 48</fullName>
            <ecNumber evidence="5">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMN2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013333"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013498.1">
        <property type="protein sequence ID" value="ENSXCOP00000013333.1"/>
        <property type="gene ID" value="ENSXCOG00000010109.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156015"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02668">
        <property type="entry name" value="Peptidase_C19L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01795">
        <property type="entry name" value="Ubl_USP48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000029">
        <property type="entry name" value="ubiquitin carboxyl-terminal hydrolase 48 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033841">
        <property type="entry name" value="Pep_USP48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044743">
        <property type="entry name" value="Ubl_USP48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF722">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="14">
        <location>
            <begin position="89"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="DUSP" evidence="15">
        <location>
            <begin position="456"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00035173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="951" mass="110103" checksum="2B4C53A7FDFC84C4" modified="2018-12-05" version="1">MAPRVKLEKAAWLWVETVKPEDIKHEHIELAYRINIPACKRGICRRNCKGNPNCLVGIGEQVWLGEIDENTFHNIDDPNSERREKNIFVGLTNLGATCYVNTFLQVWFHNLELRKSLYQCQNSRAQEHNIDSDYEPQSICEHLQYLFALLQNSNRKYIDPSGLVKALGLDTGQQQDAQEFSNLFLSLLEETLSKEKNPNLQNLIQQQFCGQFFYVTICNQCGRSSTLPSRFYELELNIQGHKNLTECITEFLKEEKLDGENRYFCENCQSKQNATRRIRLHSLPPTLNLQLMRFVFDRQTGLKKKLNTFINFPEQLDMEPFLEQKLGIYELSAVLIHRGISAYSGHYIAHVKDALTGDWYKFNDEEIEKMEGKKLQLGIEEDIAETGKTQSRKPKCSKGYHCSRNAYMLVYKVQEDESSNPSRTNVEVPSFLQKLVDQDNHKFEEWCMEMAHMRKQSVDKGRAKHEEVKELYDLLPAKDELYEFLPLEWLKKWLDDSTATKEIDNSIFLCSHGKLHPDNMGEIKRVSLEAAKLLYERYGGGPKLDGSSLCRDCVSERCRVLRLKKQLDEDYKEISNLVKRTTRYSDGYWVGKPSLRSWRQLAMEQLEEDEQEKFGMEGTAGAEDEMKTFNEDIVCTHGALSILETERKLVSTEVWTKLREYFPRAPEFTHMQEPCRQCLTLEQEEKDNEAVSKMMALEQKNQLLNLFHEKNRPVLTKWPQGTDKLYIVPLFFVEEWRKFIRRPTKSSPVSNVGNSLLLCPHGGFMFTYDSLINGEAQHIALLWPSEWNVISRLFIVDKPIAIHCVRQTESSMTQYTTQPDLCWECRESFLFQQQRDLREYTQATVYVRKVIEDKTMAKDGAPELNACSSEAEEEREELPKVDGEKDPDFSQIMHAFSVAPFDQNLSIDGKSLTDDSATLGSLGVIPECIICLKADEPVTDYAAMDDAYQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNB4</accession>
    <name>A0A3B5LNB4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enolase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNB4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010946"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011071.1">
        <property type="protein sequence ID" value="ENSXCOP00000010946.1"/>
        <property type="gene ID" value="ENSXCOG00000008266.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182805"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF5">
        <property type="entry name" value="BETA-ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="4">
        <location>
            <begin position="3"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="98" mass="10557" checksum="3AC3EC3104F3B61E" modified="2018-12-05" version="1">MSITKIHAREILDSRGNPTVEVDLWTAKGLFRAAVPSGASTGVHEALELRDGDKSRYLGKGTQKAVDHVNKEIAPQLIEKVSARGQGSSALEGFCRVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNB9</accession>
    <name>A0A3B5LNB9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LRAT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the H-rev107 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNB9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013588"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013755.1">
        <property type="protein sequence ID" value="ENSXCOP00000013588.1"/>
        <property type="gene ID" value="ENSXCOG00000010286.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156634"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016410">
        <property type="term" value="F:N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008970">
        <property type="term" value="F:phospholipase A1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070292">
        <property type="term" value="P:N-acylphosphatidylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.10">
        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051496">
        <property type="entry name" value="H-rev107_PLA/AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007053">
        <property type="entry name" value="LRAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13943">
        <property type="entry name" value="HRAS-LIKE SUPPRESSOR - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13943:SF79">
        <property type="entry name" value="HYPOTHETICAL LOC794087"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04970">
        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51934">
        <property type="entry name" value="LRAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="LRAT" evidence="5">
        <location>
            <begin position="15"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="151" mass="17601" checksum="50C166AD05993AEB" modified="2018-12-05" version="1">MGINSRPKLFPGDIVEYPRNKYFSHFGIYFGERDGVPYVAHLTCRDADTKIPLFGRALRSEVKLDPLELLGSQYKVNNMLDESHSPRDFHSVVKPAIEDMLGRDVTFDILFHNSEHQATLFRYGVKKSQQIEVVYKHIMPSWKKLFEEKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNJ4</accession>
    <name>A0A3B5LNJ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">60S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the large ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the eukaryotic ribosomal protein eL36 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNJ4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012300"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012447.1">
        <property type="protein sequence ID" value="ENSXCOP00000012300.1"/>
        <property type="gene ID" value="ENSXCOG00000009298.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011943"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1760:FF:000002">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1760">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000509">
        <property type="entry name" value="Ribosomal_eL36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038097">
        <property type="entry name" value="Ribosomal_eL36_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10114">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01158">
        <property type="entry name" value="Ribosomal_L36e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01190">
        <property type="entry name" value="RIBOSOMAL_L36E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="137" mass="15524" checksum="7C1C2F2DFE60545E" modified="2018-12-05" version="1">MQTPCRKTPGRESNSGPSFCKATVLPTAPLCNMAIRYPMAVGLNKGHPVTKNVTAPKHSRRRGRLTKHSKFIRDTIREVCGFAPYERRAMELLKVSKDKRALKFIKKRIGTHIRAKRKREELSNVLAAMRKAAAKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQE9</accession>
    <name>A0A3B5LQE9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Shisa N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQE9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010122.1">
        <property type="protein sequence ID" value="ENSXCOP00000010004.1"/>
        <property type="gene ID" value="ENSXCOG00000007590.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161304"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026910">
        <property type="entry name" value="Shisa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053891">
        <property type="entry name" value="Shisa_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31395:SF3">
        <property type="entry name" value="PROTEIN SHISA-LIKE-2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31395">
        <property type="entry name" value="SHISA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13908">
        <property type="entry name" value="Shisa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Shisa N-terminal" evidence="6">
        <location>
            <begin position="4"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="130" mass="14234" checksum="CC37104F30B4DC65" modified="2018-12-05" version="1">MSAECSSYYNTDGAFVVAFSCPKPGSAAFAVYCCGFNDVKYCCDDPNSFFPYEYGYMWWLSIGALVGLSIAAVVLLAFLITVCVLCYLFIATKPNRLDNGLPLRAPGQDGCVSDSSFCAFLKMWFLKALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LR10</accession>
    <name>A0A3B5LR10_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Pre-B-cell leukemia transcription factor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">Homeobox protein PBX1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/PBX homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LR10"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014528"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014713.1">
        <property type="protein sequence ID" value="ENSXCOP00000014528.1"/>
        <property type="gene ID" value="ENSXCOG00000010972.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165175"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003232">
        <property type="term" value="P:bulbus arteriosus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048701">
        <property type="term" value="P:embryonic cranial skeleton morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060059">
        <property type="term" value="P:embryonic retina morphogenesis in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030902">
        <property type="term" value="P:hindbrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030901">
        <property type="term" value="P:midbrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031016">
        <property type="term" value="P:pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003342">
        <property type="term" value="P:proepicardium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048741">
        <property type="term" value="P:skeletal muscle fiber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055015">
        <property type="term" value="P:ventricular cardiac muscle cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000277">
        <property type="entry name" value="Pre-B-cell leukemia transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005542">
        <property type="entry name" value="PBX_PBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03792">
        <property type="entry name" value="PBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51978">
        <property type="entry name" value="PBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 14" id="KW-0371">Homeobox</keyword>
    <keyword evidence="10 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PBC" evidence="17">
        <location>
            <begin position="36"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="229"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="14">
        <location>
            <begin position="231"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="372"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="30"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="409" mass="44743" checksum="7BF251A9AFE0A9BC" modified="2018-12-05" version="1">MDDQTRMLTGLTGLGGLSQPDVGDPDSVRKQQSLGQPQQDIGDILQQIMAITDESLDEAQARKHALNCHRMKPALFSVLCEIKEKTVLSIRGVQEEDPPDPQIMRLDNMLLAEGVSGPEKGGGSAAAAAAAAAAGGSPTDSSIEHSDYRAKLAQIRQIYHSELEKYEQACSEFTNHVMNLLREQSRTRPISPKEIERMVAIIHRKFSSIQMQLKQSTCEAVMILRSRFLDARRKRRNFNKQATEVLNEYFYSHLSNPYPSEEAKEELAKKCGITVSQVSNWFGNKRIRYKKNIGKFQEEANLYAVKTAVDAANVSAQASQANSPATPNSGSPGSNSFSHAGDTYLGQRSLNGEGLNFTPSLQLQVCKEDSCRVDGVDPHGANSWQDTTNPPSVTSPPGPPGSVHSDTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRH6</accession>
    <name>A0A3B5LRH6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Stanniocalcin</fullName>
            <shortName evidence="10">STC</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Corpuscles of Stannius protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Has an anti-hypocalcemic action on calcium and phosphate homeostasis.</text>
    </comment>
    <comment type="function">
        <text evidence="1 10">Its primary function is the prevention of hypercalcemia. Upon release into the circulation, it lowers calcium transport by the gills, thereby reducing its rate of influx from the environment into the extracellular compartment. STC also stimulates phosphate reabsorption by renal proximal tubules. The consequence of this action is increased levels of plasma phosphate, which combines with excess calcium and promotes its disposal into bone and scales.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the stanniocalcin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRH6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013662"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013830.1">
        <property type="protein sequence ID" value="ENSXCOP00000013662.1"/>
        <property type="gene ID" value="ENSXCOG00000010347.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005989"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004978">
        <property type="entry name" value="Stanniocalcin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11245">
        <property type="entry name" value="STANNIOCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11245:SF2">
        <property type="entry name" value="STANNIOCALCIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03298">
        <property type="entry name" value="Stanniocalcin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0372">Hormone</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0964">Secreted</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017440928" description="Stanniocalcin" evidence="12">
        <location>
            <begin position="22"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="220"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="285" mass="31661" checksum="DA4EA94585A5F3C1" modified="2018-12-05" version="1">MLVKLAVALLVLSVLKQAVGSDNIDFHELPPEKPASQKGRLSLQNTAEIQHCLVSAGDVGCGVFECFENNSCEIRGLQEICMTFLHNAGKFDSQGKSFIKDALKCMAHGLRHKFSCISRKCVTIKEMVFQLQRECYIKHNLCSAAKENVAVMVEMIHFQDLFPKGPYVELVNILLSCGEEVKEALTRSVRLQCEQNWGALCDSLSLCSSLVPSPASAVVEHHHHPFPSPHLEPEHPRLPRQGDKERAGKAALNAHPRNRSQGSRRHSPDAATVTEQEDPKADIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRK1</accession>
    <name>A0A3B5LRK1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRK1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012036"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012174.1">
        <property type="protein sequence ID" value="ENSXCOP00000012036.1"/>
        <property type="gene ID" value="ENSXCOG00000009106.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000203874"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="125" mass="13539" checksum="13D7977F97B076E6" modified="2018-12-05" version="1">MHGHQQRRGGHQDELESPQSDVRDWEELVVADAVAAGLLGVAGEAGLLITPNALGGNHQHQDAENEDDGEPNATDASGMPVYTADHGIKGSPVHLRLQVCRKRNKMNHSMLGLLNPPRNFYGSMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTW9</accession>
    <name>A0A3B5LTW9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Prospero homeobox 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTW9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014472.1">
        <property type="protein sequence ID" value="ENSXCOP00000014295.1"/>
        <property type="gene ID" value="ENSXCOG00000010837.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154790"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070365">
        <property type="term" value="P:hepatocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070309">
        <property type="term" value="P:lens fiber cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001945">
        <property type="term" value="P:lymph vessel development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031016">
        <property type="term" value="P:pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060042">
        <property type="term" value="P:retina morphogenesis in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.500:FF:000001">
        <property type="entry name" value="Prospero homeobox protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.500">
        <property type="entry name" value="Homeo-prospero domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023082">
        <property type="entry name" value="Homeo_prospero_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037131">
        <property type="entry name" value="Homeo_prospero_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039350">
        <property type="entry name" value="Prospero_homeodomain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12198">
        <property type="entry name" value="HOMEOBOX PROTEIN PROSPERO/PROX-1/CEH-26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12198:SF9">
        <property type="entry name" value="PROSPERO HOMEOBOX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05044">
        <property type="entry name" value="HPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51818">
        <property type="entry name" value="HOMEO_PROSPERO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Prospero" evidence="9">
        <location>
            <begin position="476"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="76"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="303"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="185"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="331"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="630" mass="71427" checksum="EC2339D94DFF5252" modified="2018-12-05" version="1">MNLSLPDQTMHSSSESCLEDDKPEVILPCFRRNMYEEPLAAYSNGSIISQLLRKTIHNKRAIDDNHFYLSNSVAAADSGQEDQSSISSKDSTVEAISPSDHVSTGVSPEEDHPLPDHLQAKRARVENIIRVMAGSPNSRQHGDGERCDTDAREMREARESCRENKRKQRLPQHQEKPGSSCNDNCNTKEEECHKLKEQLHSMQRLLRQLQEKFLQVYSQEDPEFVVREAAGADSEHDIIGKSTDACYIKSQNLQETLKQELSKAVNDCIDKVFKKVSSSTDMDISHQQRVCSTPEIQISLGADRKSLAPDHSQAEEISKSRSSELCESSEAQTSQDQTEALSLVVRKPPVTQVSSVTPTVKRPYPVHQTPFQFSYAPPLHESHILEHLLKYGPHSNFGTLPCMPPSMDRTSPDSVDLSWDAVAMRSKVTSSHFGHHHRPSSMGAVGVDGLCLPHVKMESGELQSLAERNPYMSLNISFGSRSHLKKAKLMFFYTRYPSSNVLKTFFPDVKFNRCITSQLIKWFSNFREFYYIQMEKFARQAIAEGVHDVKDITVSRDSELFRALNMHYNKANDFHVPDRFLEVAEITLHEFYNAISATKDSDSSWKKAIYKVICKLDSEIPEEFKTSSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LU79</accession>
    <name>A0A3B5LU79_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular exosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LU79"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015558"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015751.1">
        <property type="protein sequence ID" value="ENSXCOP00000015558.1"/>
        <property type="gene ID" value="ENSXCOG00000011754.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155083"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007338">
        <property type="term" value="P:single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Tetraspanin"/>
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    </dbReference>
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        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
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        <property type="entry name" value="TETRASPANIN"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TMFOUR"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0278">Fertilization</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
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    <keyword evidence="10" id="KW-0564">Palmitate</keyword>
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    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00004550"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="223" mass="24557" checksum="218988B55A0EFCE8" modified="2018-12-05" version="1">FHILTWEFLQCMFQLVFLFKPLAGTGVLAVGLWLRFDSRTKPLFEGEDSPSVFFTGVYLLIAAGGLMMVVGFLGCCGAIKESPCMLGLFFIFLLIIFAAEVAAGIWGLSNTHTEVTEFYKQTFDNYKTTKQEALKETLRLIHFGLNCCGNTGSLFDAAKDICPKQEGLAVLVTTSCPKAIDEVFNNKLHIIGGVGIGIGVIMIFGMIFSMMLCCAIKRSREFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVK7</accession>
    <name>A0A3B5LVK7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="32473.ENSXCOP00000015182"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015372.1">
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        <property type="gene ID" value="ENSXCOG00000011495.1"/>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="114" mass="12160" checksum="408AB1D9C6A14F6C" modified="2018-12-05" version="1">TAEPETSEPSHLGQENRNLKVSESRGRVDQNHCASSPHQEAPLSYVFSQYLSRLPAGARRSVQFHFDGSKVAGSQTAAQLDFAAGSVLSGSETIKPSGVQLVGQQVPTPQLLCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LX22</accession>
    <name>A0A3B5LX22_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Internexin neuronal intermediate filament protein, alpha b</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LX22"/>
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    <dbReference type="Ensembl" id="ENSXCOT00000016680.1">
        <property type="protein sequence ID" value="ENSXCOP00000016473.1"/>
        <property type="gene ID" value="ENSXCOG00000012436.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="term" value="F:structural constituent of postsynaptic intermediate filament cytoskeleton"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
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        <property type="entry name" value="Intermed_filament_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050405">
        <property type="entry name" value="Intermediate_filament"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45652:SF18">
        <property type="entry name" value="ALPHA-INTERNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45652">
        <property type="entry name" value="GLIAL FIBRILLARY ACIDIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM01391">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
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    <feature type="domain" description="IF rod" evidence="5">
        <location>
            <begin position="86"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="104"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="289" mass="33317" checksum="75DB509BC961EEE2" modified="2018-12-05" version="1">IYGSDVFSSSSYRRIFGDSPRFASSPSRVSSRGGGNRSSSLSRTSISSVGSYSRKSARSFSAPLEAFDLTQSSILNNEYKIIRTNEKEQMQGLNDRFAMFIEKVRNLEQHNKVLETELVALRQRQAEPSRLAEVYQQEIRELRSQLEELNGEKSQLVIERDSIDDDLQKLREKYEEEFRAREEAEATLKAFKKDMEVANRSNDAIRASREEANEFRRQLQSKTIEIESLRGTTESLEKQLREMEDRHNDVNVGYIIKAINNCETLIFLVKSQLYAHVAKYFYSPSAILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXW4</accession>
    <name>A0A3B5LXW4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXW4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012664"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012818.1">
        <property type="protein sequence ID" value="ENSXCOP00000012664.1"/>
        <property type="gene ID" value="ENSXCOG00000009570.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="100"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="273" mass="31034" checksum="D91AE0D99A0A1520" modified="2018-12-05" version="1">MLKLRLLNAVAPAYFFAATTVTFILHFCLFIPTIFPNPDTSLKSSTAFHTTVFLFLMFNALGNYIMTIKYPAESDNEIAIPVCSPSCSDKVDSHYLLNGRHFCKLCRKVIFRRDHHCFFTGNCIGNKNMRYFIMFCIYTSCVCMYSLVLGVAFLTVEYSISFENPLTFLTLLPLSTAYFFMGTISGLQLFLVLMLYVWLGIGLVCAGFCCQQVLLVARGQTWCQMQRGELVDNRSPWKANLKDVFGARWILGLILPVQTVEMYSEDTDGLKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYR9</accession>
    <name>A0A3B5LYR9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein yippee-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the yippee family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYR9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017038"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017258.1">
        <property type="protein sequence ID" value="ENSXCOP00000017038.1"/>
        <property type="gene ID" value="ENSXCOG00000012857.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164942"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034751">
        <property type="entry name" value="Yippee"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004910">
        <property type="entry name" value="Yippee/Mis18/Cereblon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039058">
        <property type="entry name" value="Yippee_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13848">
        <property type="entry name" value="PROTEIN YIPPEE-LIKE CG15309-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03226">
        <property type="entry name" value="Yippee-Mis18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51792">
        <property type="entry name" value="YIPPEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Yippee" evidence="5">
        <location>
            <begin position="27"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU110713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="127" mass="14518" checksum="6FE4596EFDD627D2" modified="2025-10-08" version="2">MHRPPTPPMVRMTRSKTFQAYLPSCHRTYSCIHCRAHLANHDELISKSFQGSQGRAYLFNSVVNIGCGPAEERVLLTGLHAVADIYCENCKTTLGWKYEHAFESSQKYKEGKFIIELAHMIKDNGWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M1T9</accession>
    <name>A0A3B5M1T9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RPA interacting protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027879251.1">
        <property type="nucleotide sequence ID" value="XM_028023450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M1T9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013989"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014162.1">
        <property type="protein sequence ID" value="ENSXCOP00000013989.1"/>
        <property type="gene ID" value="ENSXCOG00000010582.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114148284"/>
    <dbReference type="CTD" id="84268"/>
    <dbReference type="GeneTree" id="ENSGT00390000006416"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028156">
        <property type="entry name" value="RIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028159">
        <property type="entry name" value="RPA_interact_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028155">
        <property type="entry name" value="RPA_interact_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028158">
        <property type="entry name" value="RPA_interact_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31742:SF1">
        <property type="entry name" value="RPA-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31742">
        <property type="entry name" value="RPA-INTERACTING PROTEIN RPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14768">
        <property type="entry name" value="RPA_interact_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14767">
        <property type="entry name" value="RPA_interact_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14766">
        <property type="entry name" value="RPA_interact_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RPA-interacting protein N-terminal" evidence="6">
        <location>
            <begin position="6"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="RPA-interacting protein central" evidence="7">
        <location>
            <begin position="55"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="RPA-interacting protein C-terminal" evidence="8">
        <location>
            <begin position="141"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="25998" checksum="8F3F96C03AC861A0" modified="2018-12-05" version="1">MDALDRRRALYKGTTPPWKETYRKRCVDRLKNSRSRLLDRYRQMGDEQPRSSSIIVQEVMEEEWSALQPDDQRLPSLWGPHGVAQMFSVMKECDELAVLEEIHRELMSQEMSIIDEYERNLQFEQQYISSLVEGMDEMHIVCPICHMNNLSFSSSFISCPCGLYINTKKRNISPDVLQRLLEARVSEHMEDCLHSPVFSVTPNTDSHASLMISCQACDYLSVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3L4</accession>
    <name>A0A3B5M3L4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Telomere repeat binding bouquet formation protein 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3L4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014564"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014749.1">
        <property type="protein sequence ID" value="ENSXCOP00000014564.1"/>
        <property type="gene ID" value="ENSXCOG00000011048.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012336"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007129">
        <property type="term" value="P:homologous chromosome pairing at meiosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070197">
        <property type="term" value="P:meiotic attachment of telomere to nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028065">
        <property type="entry name" value="TERB2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35345">
        <property type="entry name" value="TELOMERE REPEATS-BINDING BOUQUET FORMATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35345:SF1">
        <property type="entry name" value="TELOMERE REPEATS-BINDING BOUQUET FORMATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15101">
        <property type="entry name" value="TERB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="192" mass="21719" checksum="7A5B927EBBF9DD5E" modified="2018-12-05" version="1">MFRCKTAWFSSSVPQDRLDFWMVEGGTIIEWRQADYLFSDDATCPDTLRIFENNDYISNKVTVFHSLFLSTCEKRQSVKSVCIGHYVLSPASVQDGDTLFLFFFNIEEPSVCYIDSSEGEGHLCDHLRSSVSCEAPGKLFNMQTYCSVPAGYISIENLPKYSGDLHDVHPGVFRCSKCKACLCLGQKYAKTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4A4</accession>
    <name>A0A3B5M4A4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Golgi apparatus membrane</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4A4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018518.1">
        <property type="protein sequence ID" value="ENSXCOP00000018287.1"/>
        <property type="gene ID" value="ENSXCOG00000013773.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160161"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000192">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF50">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0430">Lectin</keyword>
    <keyword evidence="9 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017397250" description="Polypeptide N-acetylgalactosaminyltransferase" evidence="12">
        <location>
            <begin position="19"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="13">
        <location>
            <begin position="131"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="567" mass="64594" checksum="9E8176EA57781D6C" modified="2018-12-05" version="1">MRSGWVKGLLMVFVVASALVYLSSIQTEFHSHSERLQRAQHDDSIRGQGVIRRMDRMEEDISRLSEYTSLKFDKWGDDLSEEEQKEAEGLFQQYGYNGFLSDRLPLNRDIPDTRPPRCAEKQYPENLPSLSVILIYLDEALSVIKRAIRSIIDKTPARLLREIILVDDHSSNEDLLDKLDLYVSSIHEERPGLVKKIRHSEQLGLTQARLSGWKAAVGDVVAILDAHIEVHVQWAEPLLARIKEDRTVILTPVFDKVNFDDLTVIPYRTNGDGFDWALWCLYEGLRPEWYALKDESQPAKSPSIMGILVADWTFFGEIGSLDGGMKIYGGENVELGIRVWLCGGSIEVIPCSKIAHIERFSKPYAPDLSPVMRRNALRVAEVWMDDYKYNVEVAWNLPVGNHGIDIGDVSERKKLRERLKCKPFQWYLDNVYPMLEPLQDLLSYGALINALRPDQCVDQGPVPGSTPILYGCHFFSPQVTISSSQHSDFCFLLDFVFLSLSTVITSLTENFTLGGQIQNRDTKRCLEVAMGDMGFRLVVQQCSGQRWKIQHMIIAHKQHINSEKPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4N2</accession>
    <name>A0A3B5M4N2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4N2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018330"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018562.1">
        <property type="protein sequence ID" value="ENSXCOP00000018330.1"/>
        <property type="gene ID" value="ENSXCOG00000013803.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000406"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026117">
        <property type="entry name" value="Par-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15093:SF1">
        <property type="entry name" value="PRKC APOPTOSIS WT1 REGULATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15093">
        <property type="entry name" value="PROSTATE APOPTOSIS RESPONSE PROTEIN PAR-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="235" mass="24823" checksum="DFAED35AAB44A6E2" modified="2018-12-05" version="1">MATGGFKSNAPADFLEEWKAKREKMRAKMLGDIAKATGVPLSPASSTSNSRLGAPPGTELCTNPDRSASAGNCRTSSSSPVARSPSGGTLNRPEEEAHHPAAPAATTPAAQGSNLKKAPPQMEKTWCASPDSSSMMACNNDSDKDSPSPSPSKGKEKKNAGPSARKGKGQIEKRKLREKRRSTGVVSLPSNEQSLDELDDDDAGEKGRREEEELVQANTFQNESMTADPDTGNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6N9</accession>
    <name>A0A3B5M6N9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ASPSCR1 tether for SLC2A4, UBX domain containing</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6N9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019157"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019394.1">
        <property type="protein sequence ID" value="ENSXCOP00000019157.1"/>
        <property type="gene ID" value="ENSXCOG00000014399.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042593">
        <property type="term" value="P:glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16105">
        <property type="entry name" value="Ubl_ASPSCR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17075">
        <property type="entry name" value="UBX1_UBXN9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16118">
        <property type="entry name" value="UBX2_UBXN9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000204">
        <property type="entry name" value="tether containing UBX domain for GLUT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003116">
        <property type="entry name" value="RBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021569">
        <property type="entry name" value="TUG-UBL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059238">
        <property type="entry name" value="UBX1_UBXN9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46467">
        <property type="entry name" value="TETHER CONTAINING UBX DOMAIN FOR GLUT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46467:SF1">
        <property type="entry name" value="TETHER CONTAINING UBX DOMAIN FOR GLUT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11470">
        <property type="entry name" value="TUG-UBL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50898">
        <property type="entry name" value="RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RBD" evidence="2">
        <location>
            <begin position="6"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="495"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="265"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="595" mass="64929" checksum="E7597E2320AC5E5A" modified="2018-12-05" version="1">MAASGSAVTVLTPNGRRQTIKVAPNTPLLQVLEDVCKKHGFDPNDHGLKFQRTVLDLTLPWRFANLPNNAKLEMVTSTRKQSGADSQVRIALQMEDGSRLQGSFSSGQSLWEVIAHFPQIRESELTESGATPACVYMRDEVSGEEALKKTTLKSLGLTGGSAIVSESPQPDPAPSIPEASAEVVPTSKPNPHEPLETPAVPARSSITEDVPKPQDVVRPKVQRAVEDGEEAGPSGLNSHPTSSAPAAPFIPFSGGGQRLGGLAGEIGHSLSSSSSLSAPTPTVQSPKAKKAKSNHSTKCKATVKSADRDLDNTEEFLEPVDRELLIYHMDSASRRTEDHADLPDEFFEVTMDDVRKRFAQLKSERKLLEEAPLLTKALREAQMKEKMERYPKVVLRIQFPDRHVLQGFFRPLETVGAVRHFLRSHLEDPEISFYLSSVYSLALCVCLQADLFPGALVYFGSDVKAEFYIKRDLLKTSVSALQADESIASCLLRSPETTSGSEPVEELPPPTEAMDTDPKSAQEEPEASTQPHGAKPARADPGKVPKWFKLPGEERMCSHWSKQWVCTTCSAHGCHAQGGVKMVPQKLFLVGIFCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6V4</accession>
    <name>A0A3B5M6V4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Fibroblast growth factor binding protein 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fibroblast growth factor-binding protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6V4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020225.1">
        <property type="protein sequence ID" value="ENSXCOP00000019978.1"/>
        <property type="gene ID" value="ENSXCOG00000015005.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154372"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019838">
        <property type="term" value="F:growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010510">
        <property type="entry name" value="FGF1-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15258">
        <property type="entry name" value="FGF BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15258:SF1">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06473">
        <property type="entry name" value="FGF-BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0340">Growth factor binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017179983" evidence="8">
        <location>
            <begin position="20"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="132"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="171"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="215" mass="24686" checksum="698A79C7A81AB161" modified="2018-12-05" version="1">MWTQATALLLACCLWPAEAQDERRQSIWDDPVKFQTKAKDKCTMILTGQGENIKLRLSCQGNRRAYWCEYMGRPYTCSAFNKNPRHYFVQMMWVLRKQAHACQGPREIKPHMCRKATDESQMIFSAGPFLRSRPAAQPSKQPGKPQSSAARGRPNLTMKTPFKTAWDSGKVKTTQRAKSQPTAAPTESARRRMAQQYCWRSLQGVCSFVIGWFRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M778</accession>
    <name>A0A3B5M778_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Adenosine deaminase RNA specific B1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027878380.1">
        <property type="nucleotide sequence ID" value="XM_028022579.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027878381.1">
        <property type="nucleotide sequence ID" value="XM_028022580.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027878382.1">
        <property type="nucleotide sequence ID" value="XM_028022581.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027878383.1">
        <property type="nucleotide sequence ID" value="XM_028022582.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027878384.1">
        <property type="nucleotide sequence ID" value="XM_028022583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M778"/>
    <dbReference type="SMR" id="A0A3B5M778"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019563.1">
        <property type="protein sequence ID" value="ENSXCOP00000019325.1"/>
        <property type="gene ID" value="ENSXCOG00000014535.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114147879"/>
    <dbReference type="CTD" id="558759"/>
    <dbReference type="GeneTree" id="ENSGT00940000155992"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003726">
        <property type="term" value="F:double-stranded RNA adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008251">
        <property type="term" value="F:tRNA-specific adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006382">
        <property type="term" value="P:adenosine to inosine editing"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19895">
        <property type="entry name" value="DSRM_RED1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19898">
        <property type="entry name" value="DSRM_RED1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000009">
        <property type="entry name" value="Adenosine deaminase RNA-specific B2 (inactive)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000011">
        <property type="entry name" value="double-stranded RNA-specific editase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002466">
        <property type="entry name" value="A_deamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044458">
        <property type="entry name" value="ADAR2_DSRM_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044459">
        <property type="entry name" value="ADAR2_DSRM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10910:SF58">
        <property type="entry name" value="DOUBLE-STRANDED RNA-SPECIFIC EDITASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10910">
        <property type="entry name" value="EUKARYOTE SPECIFIC DSRNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02137">
        <property type="entry name" value="A_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00552">
        <property type="entry name" value="ADEAMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50141">
        <property type="entry name" value="A_DEAMIN_EDITASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DRBM" evidence="10">
        <location>
            <begin position="75"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="10">
        <location>
            <begin position="243"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="A to I editase" evidence="11">
        <location>
            <begin position="364"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="189"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="695" mass="76585" checksum="D00E03BA9A00CD4D" modified="2018-12-05" version="1">MDVDEEENMSSSSTDVKENRNLDNVSCKDGMGVGDQLPNGSGHGSRKRPLEEGNNGHAHSKFRAKKRKKTPGPVLPKNALMQLNEIKPGLQYKLLSQTGPVHAPVFVMTVEVNGQMFEGMGPTKKKAKLNAAEKALRSFVQFPNASEAHLAMGRTLTVNTDFTSDQADFPDMLFNGFETPAPPEESFYLGSNGTNSLGEYPLPTPPGSNLVQAPLPSPSAFSSPSTGKNPVMILNELRPGLKYDFVSESGESHAKNFVMSVTVDSETFEGSGRNKKLAKARAAQAALSALFNMQLDQTPSRQPIPREGLQLHLPQVLADAVSRLVVDKFSELTDNFTSPHARRKVLAGVVMTTGTDVKEAQVICVSTGTKCINGEYMSDRGLALNDCHAEIIARRSLIRYLYTQLEFYLSNIKEDHHKSIFVPCDKGGYRLQDNVQFHLYISTSPCGDARIFSPHEAGVEDQGDRHPNRKARGQLRTKIESGEGTIPVRSSNTIQTWDGVLQGERLLTMSCSDKIARWNVVGIQGSLMSYFTEPIYFSSIILGSLYHADHLSRAMYQRIADIEDLPQQFTLNRPLLSGISNAEARQPGKAPNFSVNWTVGDQALEVINATTGKDDMGRPSRLCKHALYSRWVRLHSKLSSILRIKVPKPSSYHDAKQAAMEYHSAKKALIKAFHKAGLGAWVKKPIEQDQFTLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9J2</accession>
    <name>A0A3B5M9J2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 17">Peroxisome proliferator-activated receptor gamma</fullName>
            <shortName evidence="17">PPAR-gamma</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15 17">Nuclear receptor subfamily 1 group C member 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">PPARG</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. May play a role in the regulation of circadian rhythm.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Heterodimer with other nuclear receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 17">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
    </comment>
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    <dbReference type="STRING" id="32473.ENSXCOP00000017946"/>
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        <property type="protein sequence ID" value="ENSXCOP00000017946.1"/>
        <property type="gene ID" value="ENSXCOG00000013524.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:nuclear receptor activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:cell differentiation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:hormone-mediated signaling pathway"/>
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    </dbReference>
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        <property type="term" value="P:negative regulation of cholesterol storage"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:negative regulation of inflammatory response"/>
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        <property type="term" value="P:positive regulation of fatty acid metabolic process"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051049">
        <property type="term" value="P:regulation of transport"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:rhythmic process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06965">
        <property type="entry name" value="NR_DBD_Ppar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06932">
        <property type="entry name" value="NR_LBD_PPAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000017">
        <property type="entry name" value="Peroxisome proliferator-activated receptor gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peroxisome proliferator-activated receptor gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="1Cnucl_rcpt"/>
    </dbReference>
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        <property type="entry name" value="NHR-like_dom_sf"/>
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        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
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        <property type="entry name" value="Nuclear_hormone_rcpt_NR1"/>
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        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
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        <property type="entry name" value="PPAR-gamma"/>
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        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082:SF488">
        <property type="entry name" value="PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12577">
        <property type="entry name" value="PPARgamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01288">
        <property type="entry name" value="PROXISOMEPAR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01291">
        <property type="entry name" value="PROXISOMPAGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0010">Activator</keyword>
    <keyword evidence="9 17" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="3 17" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 16" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 16" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="18">
        <location>
            <begin position="104"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="19">
        <location>
            <begin position="206"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="RuleBase" id="RU364110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
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            <dbReference type="Ensembl" id="ENSXCOP00000017946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="472" mass="53657" checksum="3ED314162091921A" modified="2018-12-05" version="1">MVDTQQLLAWPVGFSLSTVDLPELDDSPHYLDMKHLSDYASISSSSIPSSLSPPLVSSISSAGLAYDISPPLNPIKMEPESPPQYSDNPLFSKFPDDASPSALNIECRVCGDKASGFHYGVHACEGCKGFFRRTIRLKLVYDHCDLHCRIHKKSRNKCQYCRFQKCLNVGMSHNAIRFGRMPQAEKEKLLAEFSSDMEHMHPEAADLRALSRHLYEAYLKYFPLTKAKARAILSGKTGEKVPFIIHDMKSLIEGEQFINCRQIPAQEHQLMSTLRFFKSCQSRSAEAVREVTEFAKSIPGFINLDLNDQVTLLKYGVIEVLIIMMSPLMNKDGTLISYGQIFMTREFLKSLRKPFCQMMEPKFEFGVKFNTLELDDSDMALFLAVIILSGDRPGLLNVKPIEQLQETVLHSLELQLKLSHPDSLQLFAKLLQKMTDLRQIVTDHVHLIELLKKTEVDMLLHPLLQEIMKDLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9J4</accession>
    <name>A0A3B5M9J4_9TELE</name>
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        <submittedName>
            <fullName evidence="1">Transport and golgi organization 2 homolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">TANGO2</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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    <dbReference type="STRING" id="32473.ENSXCOP00000020195"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008551">
        <property type="entry name" value="TANGO2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985">
        <property type="entry name" value="SER/THR-RICH PROTEIN T10 IN DGCR REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985:SF8">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05742">
        <property type="entry name" value="TANGO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="275" mass="30611" checksum="44E1EF000874D1F3" modified="2018-12-05" version="1">MCIIFLKFDPRPASKNAYRLILAANRDEFYNRPSKAADFWGTNSEILSGLDLEYGKEGGSWLGISKRGKLAAITNYMEGHPNPNAQGRGFLVSNYLMDKDVDSYSYLKKVSAEGHLYNGFNLITAEFKAKQDTVCYYGNRGSSEPIRLNPGIYGLSNSLLDTPWKKLLKGKEHFTNVVGDQSLSGDGLVQALLHVLNNEELNTPDPLQEQLGEGYSRSMVQALSAVCVRSPHYGTRTNTIILIDAEGNVTFTERTMLDVDTSKWSTNSFQFNLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MA68</accession>
    <name>A0A3B5MA68_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MA68"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018171"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018402.1">
        <property type="protein sequence ID" value="ENSXCOP00000018171.1"/>
        <property type="gene ID" value="ENSXCOG00000013697.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182864"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000009">
        <property type="entry name" value="PP2A_PR55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11871">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="74" mass="8287" checksum="7B56FE5EAE5CA2B3" modified="2018-12-05" version="1">MGEDAESPKINHTFLRDYVTEADVISTVEFNQTGDLLATGDKGGRVVIFQRETEIKPSNCGRSVRETRDQKDTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEN5</accession>
    <name>A0A3B5MEN5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative WW-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEN5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022020"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022287.1">
        <property type="protein sequence ID" value="ENSXCOP00000022020.1"/>
        <property type="gene ID" value="ENSXCOG00000016455.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183066"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Serine/threonine-protein phosphatase 4 regulatory subunit 1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000357">
        <property type="entry name" value="HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051023">
        <property type="entry name" value="PP2A_Regulatory_Subunit_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033461">
        <property type="entry name" value="WRNPLPNID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A 65 KDA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648:SF7">
        <property type="entry name" value="WW-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02985">
        <property type="entry name" value="HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15017">
        <property type="entry name" value="WRNPLPNID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="59"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Putative WW-binding" evidence="4">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="234" mass="25886" checksum="7B0F7BED33AE3B24" modified="2018-12-05" version="1">QVCAGNFGEFCSIVGQEATEKLLFFNLCSDSLWGIRKACAECFMTVSNSTSPEVRRSKLSPLFINLISDQSRWVRQAAFQSLGRFISTFANPSSTGLHFREDGALPEVPSSCSLNSLNCSDISSCHKDRTITHTPPNQDGRATPSPEHVATPDCEDLNHFEDGHFSVPGSATEQADDSFNSFIYWREPLPDISEELEIHGKATSDQIQKVLDCLQPHIDDPDVQGECLMPECVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MF56</accession>
    <name>A0A3B5MF56_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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            <taxon>Poeciliinae</taxon>
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        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MF56"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022327"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022600.1">
        <property type="protein sequence ID" value="ENSXCOP00000022327.1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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        <property type="term" value="C:postsynaptic density"/>
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    </dbReference>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
    <dbReference type="CDD" id="cd13241">
        <property type="entry name" value="PH2_Kalirin_Trio_p63RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000008">
        <property type="entry name" value="rho guanine nucleotide exchange factor 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047053">
        <property type="entry name" value="Kalirin_TRIO_SH3_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
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        <property type="entry name" value="RhoGEF_Guanine_NuclExch_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
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        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826:SF49">
        <property type="entry name" value="KALIRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22826">
        <property type="entry name" value="RHO GUANINE EXCHANGE FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23587">
        <property type="entry name" value="SH3_KALRN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="DH" evidence="9">
        <location>
            <begin position="40"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="227"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="391"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="359"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="454" mass="52100" checksum="6D691F8B1F390924" modified="2018-12-05" version="1">MQIIKDPNNPEVKTLNHPALDSDQGSNESDTEQRNKALRGRMFVVNEMIQSEKDYVKDLGVIVEGFMSRLEVRGIPEDMRGKDKIVFGNIQQIYDWHRDFFLVELERCVQNHDLLADLFIKHERRLHMYIVYCQNKPRSEFIVIEYETFFEEIQHEISCRMSISDYLIKPVQRITKYQLLLKDFLKYTTKAGLDCEEVEKAVELMSLVPKRCNDMMNLGRLQGYEGKLTSQGKLLQQETFCVWEQDGGVLSRSKERRVFLFEHIVIFSELLRKGSNNPGYQFKNSIKVIYLAMQESVDGDPCKFVLWSRGSAERFTLQASSASIKMTWVETIATLLDAQNNFLSALQSPIEYQRKEAGISVTRPLSSGRPPSTPPTPNGHASQPTPDSEQDDQELVLVVQDFLAVREDEITVYRGEKVQILSSNQQGQSLVYRPANTESPAAEGWVPRSVLNTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFA2</accession>
    <name>A0A3B5MFA2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MFA2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022382"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022656.1">
        <property type="protein sequence ID" value="ENSXCOP00000022382.1"/>
        <property type="gene ID" value="ENSXCOG00000016711.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154607"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="317"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="383"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="437" mass="47322" checksum="D21F6002D8FB840B" modified="2018-12-05" version="1">PDPDRSSGTAAMSPLQLSVYWRLALLFFFTPFLFFLDIFTAAIADRTCRHNNGTEASSWLGTSVCGWTDWLSYGQTVYMVGLLLGSLVGGAISDRYGKRPVLLACACVQAFCGLVPAVFPQPLLFLAIRCLTAVCCCCINICAFSLAVEWTLPAARLWPPAFLPFCFSLGTMGGAPLAWLSPSWTYLHLLLALPQIVCLPLYLYAPIPESPRWLFNSPADQKCLDLVKLSASEAQTARGHAPSDITQLRHPTILLRLFIMSYLSAATALTYYGICLNIGSFGVGVYAAQFFSGLSEAPCLLVPLIRIGRRLISMLALFLSGTCLDFLALLGKLCILAATFISVLYCIELFPTGVRQLCISLVNLSYRLGCLVTTFVPPSPSGAISLAAMIVYSSGPIVGCGLCLLLPETSGAPLPDSLEDCNRQPKNQPASALWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGH4</accession>
    <name>A0A3B5MGH4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Carbonic anhydrase</fullName>
            <ecNumber evidence="3 8">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>hydrogencarbonate + H(+) = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="3 8"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGH4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020211"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020458.1">
        <property type="protein sequence ID" value="ENSXCOP00000020211.1"/>
        <property type="gene ID" value="ENSXCOG00000015149.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166576"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015670">
        <property type="term" value="P:carbon dioxide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006885">
        <property type="term" value="P:regulation of pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.200.10:FF:000001">
        <property type="entry name" value="Carbonic anhydrase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018338">
        <property type="entry name" value="Carbonic_anhydrase_a-class_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF120">
        <property type="entry name" value="CARBONIC ANHYDRASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00162">
        <property type="entry name" value="ALPHA_CA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="249" mass="27676" checksum="DF445EA2899F1903" modified="2018-12-05" version="1">GPDKWADSFPIANGPRQSPIDIVPGEASYDPALKPLVLKYDPSTCLEILNNGHSFQVTFLDDIDSSTLQDGPILFNHRLKQFHFHWGASDDHGSEHTVARTKYPAELHLVHWNTKYANFGEAASQPDGLAVVGVFLKIGEKNPNLQKLIDAFDSIKTKGSQCSFADFNPETLLPGCLDYWTYEGSLTTPPLLESVTWIVCKEPISVSSEQMAKFRSLLFSGEGETECCMVDNYRPPQPLKGRTVRASFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGP2</accession>
    <name>A0A3B5MGP2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DEP domain-containing protein 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEPDC7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGP2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023061.1">
        <property type="protein sequence ID" value="ENSXCOP00000022785.1"/>
        <property type="gene ID" value="ENSXCOG00000017026.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182976"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04405">
        <property type="entry name" value="RhoGAP_BRCC3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16206">
        <property type="entry name" value="DEP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16206:SF9">
        <property type="entry name" value="DEP DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEP" evidence="3">
        <location>
            <begin position="18"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="421" mass="46743" checksum="52DD80F2502D080F" modified="2018-12-05" version="1">MSASAQSSSIWSDLIAHLRSKVTVKRRLVQFKAHSDCFVGSEAVDVLLRHAANANGGAVSREQVVCVCQALLQSGALEAVGTRNRRQDAFEDSGSALYRSVVWQEQTLLRLLNLVELPLLEGVLQCSQSPTSDPSANLLPHRNPDLISNSLDRRILQAFRDSQEDNWLTSGLDCLDFLPDQQVAVLSRELPEDDLPDRPPAPGQLLLFGSLVRHYGSRPPLLPSNMAAVYTAVAELLENGQLEKALEALQLCLKLLPPGCREELRRLLTFMAAAAEPRAPKLDKEVENRAAVMKAFSKAILHSRSFPKEKQDLILNFLLSNRQEVFKIPGSLHKTISEKLAMLAQTDAPGNMMLQVTYRTRQNCTLGTQQALLELLSSINLDRSMAAKERRRLLRQFCSAHPEIFHQYFGDSAASQISANF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIE7</accession>
    <name>A0A3B5MIE7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cAMP-dependent protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.11" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIE7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019334"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019572.1">
        <property type="protein sequence ID" value="ENSXCOP00000019334.1"/>
        <property type="gene ID" value="ENSXCOG00000014542.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166522"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034237">
        <property type="term" value="F:protein kinase A regulatory subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14209">
        <property type="entry name" value="STKc_PKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000005">
        <property type="entry name" value="cAMP-dependent protein kinase catalytic subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044109">
        <property type="entry name" value="STKc_PKA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF135">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0114">cAMP</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="6 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="36"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="287"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="339" mass="39468" checksum="A325CAB3456A8906" modified="2018-12-05" version="1">CFSHPPTVKEFLAKAKEDFLKKWENPSQNTASLEQFERLKTLGTGSFGRVMLVKHRETGQHYAMKILNKQKVVKLKQIEHTLNEKRILQAVSFPFLVRLEYSFKDNTNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHALDLIYRDLKPENLLIDQQGYIQVRDSVSSLTIFWCLCPGFHFCLCCFICPGYNKAVDWWALGVLVYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKGHKWFATTDWIAIYQKKVEAPFVPKFKGPGDTSNFDDYEEEEIRVSFTEKCAKEFAEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJB4</accession>
    <name>A0A3B5MJB4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJB4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019639"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019884.1">
        <property type="protein sequence ID" value="ENSXCOP00000019639.1"/>
        <property type="gene ID" value="ENSXCOG00000014752.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244848"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030593">
        <property type="term" value="P:neutrophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000355">
        <property type="entry name" value="Chemokine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF930">
        <property type="entry name" value="C-X-C CHEMOKINE RECEPTOR TYPE 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00657">
        <property type="entry name" value="CCCHEMOKINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="60"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="355" mass="40093" checksum="A408FF751E5CEAD6" modified="2018-12-05" version="1">MSSFVDDFSSIYEELNFTYNYTEFILNPATEPCDNFRFPEVMMLIACLFYVFVFLLAIPGNLVVGLVIGLSKQALTPSDLYLLHLAVADLLLAFTLPFWATSVTSGWVFGDLMCKIVTVLQELSFYSSILFLTCISMDRYMVIVRAMEARRANRQVCSWVICVVVWVVGGLLSLPGFFSSAFPSRNTTHMVCAVQYNPSSADEWRLGTRVLRHLLGFVIPLAVMLPCYGVTIQRLLHVRGGFQRQRAMRVIICVVVAFLLCWTPYHLAVMADTFFRLKIVEYGCQERTAVDQAMFATQSLGLLHSCVNPVLYAFVGEKFRKKLLQKMRKMGLLERASISRASRSSISSEITSTVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKE2</accession>
    <name>A0A3B5MKE2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Growth/differentiation factor 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Myostatin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Acts specifically as a negative regulator of skeletal muscle growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027857922.1">
        <property type="nucleotide sequence ID" value="XM_028002121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKE2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024844"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025145.1">
        <property type="protein sequence ID" value="ENSXCOP00000024844.1"/>
        <property type="gene ID" value="ENSXCOG00000018549.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114135141"/>
    <dbReference type="KEGG" id="xco:114135141"/>
    <dbReference type="GeneTree" id="ENSGT00940000166043"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19388">
        <property type="entry name" value="TGF_beta_GDF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000003">
        <property type="entry name" value="Growth differentiation factor 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000006">
        <property type="entry name" value="growth/differentiation factor 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF166">
        <property type="entry name" value="GROWTH_DIFFERENTIATION FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="4" id="KW-0202">Cytokine</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 13" id="KW-0339">Growth factor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017457656" description="Growth/differentiation factor 8" evidence="14">
        <location>
            <begin position="22"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="15">
        <location>
            <begin position="277"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="389" mass="44279" checksum="F2DA66CBBE6A997A" modified="2018-12-05" version="1">MPRYNFFPCLLALIFLGLSGSDEPNVLLPPASEPPTDAGLSLLDEDAGSHECSACVWREQSKVLRLETIKSQILSKLRLKQAPNISREVVNQLLPKAPPLQQLLDHHDFQGDASAQDEFMEEDEYHATTESVITMASEPEPLVQVNGKPSCCFFKFSPKLMFTKVLKAQLWVYLRPLQQTSTVYLQILRLKPVTEQGSRHIRIRSLKIELNSRAGHWQSIDFKHVLQNWFKQPHTNWGIDINAFDESGNDLAVTSLRPGEEGLQPFLEVKVLETTKRSRRNLGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGQCEYMFMQKYPHTHLVQHANPRGSAGPCCTPTKMSPINMLYFNDKQQIIHGKIPGMVVDRCGCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLB5</accession>
    <name>A0A3B5MLB5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MAP7 domain containing 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAP7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLB5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025494.1">
        <property type="protein sequence ID" value="ENSXCOP00000025189.1"/>
        <property type="gene ID" value="ENSXCOG00000018816.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182941"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051483">
        <property type="entry name" value="MAP7_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008604">
        <property type="entry name" value="MAP7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15073:SF17">
        <property type="entry name" value="MAP7 DOMAIN CONTAINING 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15073">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05672">
        <property type="entry name" value="MAP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="193"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="528"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="40"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="361"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="193"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="235"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="248"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="289"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="319"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="650" mass="74025" checksum="69508D8ABF8D6307" modified="2018-12-05" version="1">MNSEQRQKLAKERREERARYIAAKKAQWLEKEEKARRLRESQLEERRRKLEEQRLKAEKRRALLEEKQRQKLEKNKERYEAAIKRSTKKTWAEIRQQRWSWAGGLNQTSRRESRCSASTVNLPRQVEPVINNRLSKSSATLWNSPNRTRSLRLSPWESRIVERLMTPTLSFLARSRSAFIRPLCARCCHLQVDKKKKEKKDKERENEKEKNALAKERVQKKRQMTSSVSASVSRSRPEARYRPDPERLPSSSSLLRSAPRARNRPPSPAPRSRPLSPLVPTSKPPPGKKTPSAATKPRPKRAQTPARVQPQAVAAVTVETREETQRTDDLAEKNTAASAAPCWLQVELVRASPLVCVSVLQRDEAEAKAREEAERQREEREKHFLKEEQERLERKKRLEEIMKRTRRSDAGEKVGVILTIINDVSTAAATLLGPGPPAPGPPLTGPVTVNGVQPASHQNDVSANGELERRAFEGAEPYLRKAGAMKPQHVAGSQSERSFHQVKEKVNKLIILSAVYLQRFCESPWRPPGGHAPLRPHPSDQQSAKHGGTETLKLYVTSCSSAEPGKVFLPAAAKTERLQLIGRNPTERLQLIGRLAPPDLTLEQRQWLLLLFCCCSCLLQFYRVWGRGGSVCVRVRMRVCVFLLLFKPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MMF0</accession>
    <name>A0A3B5MMF0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NR LBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MMF0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024736"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025037.1">
        <property type="protein sequence ID" value="ENSXCOP00000024736.1"/>
        <property type="gene ID" value="ENSXCOG00000018479.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183038"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035259">
        <property type="term" value="F:nuclear glucocorticoid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071376">
        <property type="term" value="P:cellular response to corticotropin-releasing hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003070">
        <property type="entry name" value="NR4A1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24085">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24085:SF1">
        <property type="entry name" value="NUCLEAR RECEPTOR SUBFAMILY 4 GROUP A MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01284">
        <property type="entry name" value="NUCLEARECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="NR LBD" evidence="12">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="211" mass="24190" checksum="A530F12D4DC30D53" modified="2018-12-05" version="1">MSDSLKEKEDAGDIQQFYDLLTGALDVIRNWAQTIPGFMDFCAEDQELLLESAFVELFILRVAYRSKPEKNKLIFCNGLVLHRQQCANSFGGWIDSIMDFSQSLHRMNLDNSLIACLSAFVIITDRHGLKEPKRVEDFQSNLISCLREHMSGIGSEVTRTQPNYLSRLLGKLPELRTLCTEGRQRIFYLKLEDLVPPPPIVEKIFMDTLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNK2</accession>
    <name>A0A3B5MNK2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Immunoglobulin superfamily, member 9b</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000021059"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021318.1">
        <property type="protein sequence ID" value="ENSXCOP00000021059.1"/>
        <property type="gene ID" value="ENSXCOG00000015755.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165064"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000323">
        <property type="entry name" value="Immunoglobulin superfamily member 9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF41">
        <property type="entry name" value="PROTEIN TURTLE HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="19"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="146"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="236"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="331"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="427"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="6">
        <location>
            <begin position="516"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="752"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1021"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1153"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1355"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1390"/>
            <end position="1452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1622"/>
            <end position="1641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1701"/>
            <end position="1727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1743"/>
            <end position="1820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="759"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="793"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="837"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1168"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1204"/>
            <end position="1214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1215"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1240"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1268"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1391"/>
            <end position="1401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1417"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1705"/>
            <end position="1719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1743"/>
            <end position="1767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1787"/>
            <end position="1799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1801"/>
            <end position="1820"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1820" mass="201904" checksum="764DFF2B454D8B02" modified="2018-12-05" version="1">MLVFVEGGWWVVGLIVLVPHTMCIPQGVVRGWLDQTQREGDSAELDCSLTPSSKEATTPNLFPLHVVEWVRLGYNVPVLIKFGVYAPRVHPNYKGRVSLTHGASLLVERLTLEDEGWFECRILLLDSKTDNFQNGTWTFLSITAPPVFIKTPPTFVEVLLGDSLTLSCGAHGNPRPTVVWHKDESPIEKHEKIKVLNGTLSLATVTRNVSGVYKCHVSNTEGNLTHYTELQVQGPPIIIISPEDTTLNMSQDAVLQCQADAYPSNLTYEWLKQGQNVYHIESLRSRVKILVDGTLLIPNLIPEDAGNYTCIPTNGILTPPSASAHLKVKHPARVGRMLRETYLPAGMEGVIFCPVQADPPVLYVNWTKDGNELNLDNFPGWMVNSEGSVFIATANDNAVGMYTCTPYNSYGTMGQSEPTKVILQDPPSFRVPPLPEYLQEVGRELIIPCEASGDPAPNVTWSKIGPSPRSPYTVLSNGSLLLQPLSKDHHGGWECLATNRVATVSAGTVVFALGTSPHAVSSVTVTTEMNQANVSWVPGFDGGFTQKFTVWVKQTSRGKHEWASLPVPTSKNYLLVTGLLAGTGYQFSVLPQNKLGSGPFSEIVSIRTKGLFLAVPTEVPKTSTGVPALDPPTLLSANRTGRGVLLQWQPPEAPLSPLTGFVLQARRDQGHNKVLSEPSDDINITGYLLHLFIKHRIIPEPLMAGVIGGACFLLLAIILSLVTACYMSNRRRPDSVLKFKLCPPLPFFPNSSLSQSDRSSFDKGSRGEYHDQRKQLLSNSSPPPHYTLFESHLGSQAPSPTALESISRGPDGRFIVQPLPEVSSPSTKNIKKDVLQVNGSVHGSGSNRTSIRDSPKSSILSSEKDERKDSPLTVDVPELSRPPASPGRVRAMARNFSRHGCFYSDDEQGSETLLERASFYSDSSERKPSDSLRRCRMRGNTEDLFSSLGRRTMLLDRDRLHHGGYRPMESHLTDNSTLVSQLESELEMDSVNKCVQLAKEREEIERELKNFAMDQRHCSRVRDAPSPCSTTERPQRDETESVEEPVWKPQEVTIRPKYRPTGVTSRVSDYRRACYFGNTSSPLDRLPTSHIQWDVSPVTSVTSLIPVQTPCKTTSLISSREATEVSLPLDSSSSPVTQNTSLPMLSRDVTLDKATSSNLQKNKEACTKSGTEQNIVEETQELGVVSKSTSPLDSQLRDLPNTVRPETSDSVQHNQTERTADIGHKTSRDPSPCSSSTLLYDHHKVGAKSKDRETQDHDDQHVSGFYSDLEKESVRTRSRRSDKCLFPDSPSPIPTLTLVEEVESDQSQFLVPRMSDCFKAKPAAPSPKMSPHQTSAILEYLSLPGFIEMSVDEPVEEGQVTDATTKQTPKEESVVAKPDVVPRNWEVHVQENPQALSSQKQGDPLGSADSSYDAYKTQGNRRSPQVTARVRFPDDPRPSSSGPEKTSKELYNEKTRIRAGSIYTVRPESRLGSRSAHTLFNAAKGMADIVSKHSQSCADSDEFLSEQPQRQASQGNRTNKIVSRITQAPVPFLKKSLSLGPCRTLSGVAQPRPYLKKSISLGSQRWEHFESPRPYISEKCYWDEFPNPDVRVKSYSLGRTPPSLPRPGPCWKEYIPFRRPSMGSLERPHHTQRSLASPSYLTPLYPPRQTSMSPMLEPSDPRRQAAIFSESSRWSPSFQDTLRSVQHQFIPMPTSNSLPYYQHWPDPRGEGLRHSDSRRGPPRSYLPRGISWPSPYYSAFPPREGDGYRHPDRMMGRSGDAEFRDGGRASYASQSSGRGSAGLFRQSLSVTPTLLSSPETTEEHERHRAEMELAERRVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPS3</accession>
    <name>A0A3B5MPS3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">E3 ubiquitin-protein ligase MIB1</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">E3 ubiquitin-protein ligase mib1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18 19">RING-type E3 ubiquitin transferase MIB1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
            <location evidence="2">Centriolar satellite</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MPS3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021479"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021741.1">
        <property type="protein sequence ID" value="ENSXCOP00000021479.1"/>
        <property type="gene ID" value="ENSXCOG00000016077.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:centriolar satellite"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:endocytosis"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="E3 ubiquitin-protein ligase MIB1 isoform X1"/>
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    <sequence length="996" mass="109251" checksum="11153874744B8D83" modified="2018-12-05" version="1">MTTGRNNRAMMEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDIRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTTCYHGDKHHLRHRFYRITTPGSESRVLLESRRKSKKITARGIFAGGRVVRGVDWQWEDQDGGNARRGKVTEIQDWSAASPHSAAYVIWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVTAGAEGGNSQFHVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKIAPSGSAVSNASGERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHNVDLEAEDKDGDRAVHHAAFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNASLDIQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKSSGQVDTRSPSLNSNIESLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRTPYVLCCGGKASGNIPALQRDKDNTNVNADVQKLQQQLHDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRVLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQ68</accession>
    <name>A0A3B5MQ68_9TELE</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSXCOP00000026554.1"/>
        <property type="gene ID" value="ENSXCOG00000019824.1"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:microtubule cytoskeleton organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="CINGULIN"/>
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    </dbReference>
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        <property type="entry name" value="CINGULIN-LIKE PROTEIN 1-RELATED"/>
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    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
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    </feature>
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        <location>
            <begin position="162"/>
            <end position="229"/>
        </location>
    </feature>
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            <begin position="79"/>
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        </location>
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            <begin position="105"/>
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        </location>
    </feature>
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            <begin position="130"/>
            <end position="142"/>
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            <end position="203"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="335" mass="36369" checksum="DA72D6CDF986C71E" modified="2018-12-05" version="1">MGTPSTDRKAQLDYGVQIRFIKDLHDTGGVPLEKSRANSNATPPSNKYGVAVRVQGISGQPYVVLKDGEKGDSYGVQLSNPNPTSGPTSPYNSIPNHSKEPADFPNSSSPVVNSSRSPVKLTGLITRNSPPKPTPEPPPPTESEPAAAIDTNSLAPINKLINKFNSSTPGGTLQARGRTGARQRLQFDERKRSRSLDARKDAQPEAIPASPSSPALNPHAAPISAASSPQSKNIFDSQFSGRSSLCFLSSSSETEASFKRKSSLVFETPDRLQVKSRFVVPENILQILHVFLCTYATYVLLVIRTKIATYKPRRLHFGEKKKCDCLPHHDVKSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRA5</accession>
    <name>A0A3B5MRA5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRA5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024010.1">
        <property type="protein sequence ID" value="ENSXCOP00000023726.1"/>
        <property type="gene ID" value="ENSXCOG00000017717.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160396"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000029">
        <property type="entry name" value="Complement C1q tumor necrosis factor-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF52">
        <property type="entry name" value="C1Q DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="C1q" evidence="7">
        <location>
            <begin position="75"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="33"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="214" mass="23599" checksum="E56D3AD8ECD0F9A8" modified="2018-12-05" version="1">MPPAEGSGDPPALAEFSHVPEIRTYINMTILKGDKGDRGDRGTPGNVGHEGPPGAQGVAGSKGSKGHAGPPGDPCKLSHSAFSVARRKSLHSDDAYQAVIFDTVFVNLGGDFEMFHGKFFCRVAGVYFFNVNIHTWNFKETYLHVMQNDAERAIVYAQPSDRSIMQSQSVMLELRPRDAVWVRLFKRERENAIYSDDVDVYVTFNGYLIKAGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRL9</accession>
    <name>A0A3B5MRL9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Malignant T-cell-amplified sequence</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the MCTS1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRL9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022139"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022408.1">
        <property type="protein sequence ID" value="ENSXCOP00000022139.1"/>
        <property type="gene ID" value="ENSXCOG00000016543.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074964"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11609">
        <property type="entry name" value="MCT1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21155">
        <property type="entry name" value="PUA_MCTS-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.400.20:FF:000001">
        <property type="entry name" value="Malignant T-cell-amplified sequence 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016437">
        <property type="entry name" value="MCT-1/Tma20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041366">
        <property type="entry name" value="Pre-PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798:SF0">
        <property type="entry name" value="MALIGNANT T-CELL-AMPLIFIED SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798">
        <property type="entry name" value="MCT-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17832">
        <property type="entry name" value="Pre-PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005067">
        <property type="entry name" value="Tma_RNA-bind_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="6">
        <location>
            <begin position="102"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="190" mass="21174" checksum="CF3B4DFD4AF9FD36" modified="2018-12-05" version="1">GGRGGGGIEGLGRFDEKENVSNCIQLKTSVIKGIRNQLLEQFPDIEPWLNQIMPKKDPVKIVRCHEHIEILTVNGELLFFRQREGPFYPTLRLLHKYPFILPHQQVDKGAIKFVLSGANIMCPGLTSPGAKLYPAAADTVVAIMAEGKQHALCVGVMKMSAENIEKVNKGIGIENVHYLNDGLWHMKTYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MS13</accession>
    <name>A0A3B5MS13_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MS13"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023976"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024261.1">
        <property type="protein sequence ID" value="ENSXCOP00000023976.1"/>
        <property type="gene ID" value="ENSXCOG00000017904.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159578"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF10">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C MEMBER 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="14"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="97" mass="11054" checksum="44D6B91B8FD7DD1E" modified="2018-12-05" version="1">MLYRYDRVLDVCKRGLNLSGGQKQRISLARAVYANKDIYLLDDPLSAVDAHVGKHIFEECIKKELQGKSVILVTHQLQVGSHKLFAKWDKNCRVEGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSR7</accession>
    <name>A0A3B5MSR7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Deubiquitinating enzyme A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C85 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSR7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022549"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022824.1">
        <property type="protein sequence ID" value="ENSXCOP00000022549.1"/>
        <property type="gene ID" value="ENSXCOG00000016856.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158963"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061578">
        <property type="term" value="F:K63-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22752">
        <property type="entry name" value="OTU_OTUD5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF4">
        <property type="entry name" value="OTU DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="OTU" evidence="10">
        <location>
            <begin position="10"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="185"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="196"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="319"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="330" mass="37038" checksum="C630B43800480F9E" modified="2018-12-05" version="1">NPGSVFSDQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRPVEVYQYSTEPINTFHGIHQNNDEPIRVSYHRNIHYNSVVNPNKATIGVGLGLPAFKPGYADQSLMKSAIKTSEESWIEQQMLEDKKRATDWEATNEAIEEQVARESYLQWLQDQEKQARQPRKASATCSSATAAASSGPDDWSARSPRQRDSDPSHSDLTAAAPAGNKPPSPTGAALILSKPPSPCAPGPSNQSRHHLEYRAIMQEMSPTAFGLTDWEDDEILASVLAVSQQEYLDSMKHQSASAAMHREREPSPDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MTN5</accession>
    <name>A0A3B5MTN5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NDRG family member 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDRG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MTN5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027774"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028109.1">
        <property type="protein sequence ID" value="ENSXCOP00000027774.1"/>
        <property type="gene ID" value="ENSXCOG00000020710.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182872"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060038">
        <property type="term" value="P:cardiac muscle cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045162">
        <property type="term" value="P:clustering of voltage-gated sodium channels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001947">
        <property type="term" value="P:heart looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004142">
        <property type="entry name" value="NDRG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11034">
        <property type="entry name" value="N-MYC DOWNSTREAM REGULATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03096">
        <property type="entry name" value="Ndr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="295"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="331" mass="36283" checksum="CC34A36CE9D514AD" modified="2018-12-05" version="1">MPECWDGEHDVETPYGMLHVVIRGAPKGNRPAILTYHDVGLNHKMCFNPFFNNEDMQEITKHFVICHVDAPGQHVGASQFPQGYQYPTMDQLAGMLPTVVQHFGSVIVGIGVGAGAYILAKFALLFPDMVEGLVLLNIDPNGKGWIDWAASKVPLFNHISNSSLPKVSHLCFVCAQDEMVNNTELVQSYRQQITNSINHQNLQLFWNLYNSRRDLEMNRGGTTINAKTLKCPVMLVVGDNAPAEEGVVECNSKLDPTNTTFLKPGKLTEAFKYFLQGMGYMPSASMTRLARSRTASLTSAGSMEGSRSRACTNSDSGEGVGAVSQTMEVSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MV99</accession>
    <name>A0A3B5MV99_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fucolectin tachylectin-4 pentraxin-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a defensive agent. Recognizes blood group fucosylated oligosaccharides including A, B, H and Lewis B-type antigens. Does not recognize Lewis A antigen and has low affinity for monovalent haptens.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fucolectin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MV99"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027814.1">
        <property type="protein sequence ID" value="ENSXCOP00000027481.1"/>
        <property type="gene ID" value="ENSXCOG00000020517.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01060000248575"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042806">
        <property type="term" value="F:fucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010185">
        <property type="term" value="P:regulation of cellular defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001868">
        <property type="term" value="P:regulation of complement activation, lectin pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051941">
        <property type="entry name" value="BG_Antigen-Binding_Lectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006585">
        <property type="entry name" value="FTP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45713:SF6">
        <property type="entry name" value="F5_8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45713">
        <property type="entry name" value="FTP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22633">
        <property type="entry name" value="F5_F8_type_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00607">
        <property type="entry name" value="FTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fucolectin tachylectin-4 pentraxin-1" evidence="8">
        <location>
            <begin position="34"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="173" mass="18624" checksum="5BEB794D3057FEAB" modified="2018-12-05" version="1">MEFKSSGFLLQIDFFSHLANFKACTPLVYPVESLENVAPEGIASQSSTAFLGVASRAVDGNRDPEYSHGSCTHTGGEPNPWWSLLLPVVYRVSTVSITNRKVASERLNNAEILIGNSLENNGKNNPRCAVISSIPPGGTKSFNCTGMTGRMLTVKISTSYGLLTMCELEIYGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVL1</accession>
    <name>A0A3B5MVL1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + UDP-N-acetyl-alpha-D-glucosamine = [(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9595"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the chitin synthase family. Class IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVL1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025357"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025664.1">
        <property type="protein sequence ID" value="ENSXCOP00000025357.1"/>
        <property type="gene ID" value="ENSXCOG00000018940.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064569"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000139">
        <property type="entry name" value="Chitin synthase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF42">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03142">
        <property type="entry name" value="Chitin_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017248562" description="chitin synthase" evidence="14">
        <location>
            <begin position="21"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="330"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="743"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="772"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="804"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="835"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="863"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="905"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1014"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1058"/>
            <end position="1078"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1113" mass="127267" checksum="0B5C6A5A55962894" modified="2018-12-05" version="1">LKRLFIFQASFLLLITLSHEESKTLQAEQKPLTLLCIGCSLIAPAVLLMLKSFWKACYKSSKLPRKSSVLFFEFLVSVGLAVLTIVAMPHLDIVTNVTILNSVAVLSAFLQAVAQCTAKRMNIFLLPSIMASVVMVVGYCLFVVLYILKDPTDVKTAIWVGLAVGGSILVSFNWWENYFRVICVNSKSNILKNLFEDLTKSQNMLHIMSSLLRIVVTACVLGAYVPLAKMDWDIVTSIPSRETRIVVIIIGVQLISSALCQWFALAACKMHAVRRCFIVPLYLASLAVMVLFIVPVILDYQDHQLILNTTENINFTRYCVDAVIVRNQSLTLVVVTFFSVSFLSGAAASWWIGLVLATIHIWFFKIYRIQKTQDLFGRRLYEGAFIEQSLLLNTRFNIRTRKSRKKNKEDATPALIYLCATMWHETYEEMMNIMISIFRLDQYRPKTEPKFNDFTFEAHIYFDDAFENAKGNQDRDLNTYAKDLVQIITEQQIPEQSIIETPYGARLVVEMPHGNNIVAHFKDKQKIRNKKRWSQVMYLYYLLGVECIKNKTVHSFKREKHNTYLLALDGDTDFQPAAVMLLIDRLKMYPRVGAACGRIHPTGSGPAVWFQKFEYAVSHWLQKTAEHVFGSVLCSPGCFSLFRAEALMDDNVMKKYSTTSTEPSQYIQYDQGEDRWLCTLLLKQGWRVEYNAASDAYTNAPQDFKELYNQRKRWGPSTMANVIDLLGSTNLVSKKNPSMSKPFMFYQLFAMISAILAPATICLMVAGSLSFILDIPSAAALVIAVIPPAIYLGLCFKLKADTQIIIAAVLSIMYAFLMMVVTMSIIASMVKEQTILTPSSIFIMIMSIIYIVTALLHPQELPLLFYGLLYIICIPSAYLLLTIYSMVNMNSVSWGTRETKPTAEAATTAALIFLQNKLKLSLTFTAKNTFKRLCSQMKCCKKSNQRVEDEQLTRRQEIPEPMQLEPQPQNSIDEERFWKELIEKYLEPLQNDKEKQEKTKNELQDLRNKINFSFFFLNALWLVAAFVFQVFKVFQIQFDIVDLNLNKTGKQFQIEPMSLMFILGFAISVFLQFIGMLYHRIMTLIHYVAFLETEPKQQKSARTEVNIFVYYSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVS4</accession>
    <name>A0A3B5MVS4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clathrin/coatomer adaptor adaptin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVS4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023664"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023947.1">
        <property type="protein sequence ID" value="ENSXCOP00000023664.1"/>
        <property type="gene ID" value="ENSXCOG00000017674.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157603"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="6">
        <location>
            <begin position="48"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="190" mass="20872" checksum="134245B5240F2B4E" modified="2018-12-05" version="1">MLIMSTTALYNDQQGGAEAAVEAGQESTATSSSSGAAFGLFSTDFKKNEDLKEMLESNKESLKLEAMKRIVQLISKGKNASDLFPAVVKNVASKNIELKKLVYVYLVRYAEEQQDLALLSISTFQRALKDPNQFIRASALRVLSSIRVPIIVPIMMLAIKEAASDLSPYVRKTSAVSLFLSAWTLTRRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWC8</accession>
    <name>A0A3B5MWC8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Homeobox D10a</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Abd-B homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027877694.1">
        <property type="nucleotide sequence ID" value="XM_028021893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWC8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025707"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026016.1">
        <property type="protein sequence ID" value="ENSXCOP00000025707.1"/>
        <property type="gene ID" value="ENSXCOG00000019210.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114147460"/>
    <dbReference type="KEGG" id="xco:114147460"/>
    <dbReference type="CTD" id="30404"/>
    <dbReference type="GeneTree" id="ENSGT00940000158266"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000018">
        <property type="entry name" value="Homeobox A10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046333">
        <property type="entry name" value="HXA10/ABDB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45874">
        <property type="entry name" value="HOMEOBOX PROTEIN ABDOMINAL-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45874:SF5">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-D10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="251"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="253"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="152"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="211"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="327" mass="36795" checksum="AA395AEC1860A332" modified="2018-12-05" version="1">MSFPSSSPATNPFLVDSLIGACRTDSFYSNSSMYMPSGSDMHGTYGMQTCGLLPSFGKRGEVGPQNTAMSVHSYIPQMDTWTDPSRTCRTEQMSNCTFPQSIKEESNCCMYSDKRVQKEGYSNVIGEPCPVEGPEIPVPGYFRLSQTYANGKHPENYCPEPPSPNPTLAQLSRVTPKPQHATSSSSSSGFAEAEKNVRDPETITSTPNRGESPDPVKSGSEEKTEASSPELQKEGKDCKSDALTNNWLTAKSGRKKRCPYTKHQTLELEKEFLFNMYLTRERRLEISRSVNLTDRQVKIWFQNRRMKLKKMNRENRIRELTSNLTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWN1</accession>
    <name>A0A3B5MWN1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 35 member A5</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWN1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024149"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024438.1">
        <property type="protein sequence ID" value="ENSXCOP00000024149.1"/>
        <property type="gene ID" value="ENSXCOG00000018028.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182827"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015165">
        <property type="term" value="F:pyrimidine nucleotide-sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007271">
        <property type="entry name" value="Nuc_sug_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10231">
        <property type="entry name" value="NUCLEOTIDE-SUGAR TRANSMEMBRANE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04142">
        <property type="entry name" value="Nuc_sug_transp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005799">
        <property type="entry name" value="UDP-gal_transpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="349" mass="38969" checksum="499B574A29108277" modified="2018-12-05" version="1">MVSCCPRPRLCSRSSAYTLALGLGFVTLGTSRIVLLKFSANAENKYDFLPASVNLLAEALKLLFCLVMSVRVIVREGRSCRDLGCASSSSFLISLKWAVPAFLYFLDNLIIFYVMTYLQPAMAVLFSNFVILTTAVLFRLVLKDRMVEKLQTLGVGHVLLLLQCFISAMANIYNEKILKEGDQLTESIFIQNSKLYVFGVVFNALTLGLSSEARGLTIHCGLLHGHNVYSLALVLVTAALGLSVAFILKFRDNMFHVLTGQITTVLVTGFSLFLFDFRPSLDFFLQAPMVLLSIFIYNASRPRDPEYSLQREKLRVINGEVFERSRGDGEELELLTKTNTDSESDEESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MYP1</accession>
    <name>A0A3B5MYP1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peroxisomal biosis factor 11 alpha</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MYP1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027122"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027455.1">
        <property type="protein sequence ID" value="ENSXCOP00000027122.1"/>
        <property type="gene ID" value="ENSXCOG00000020248.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014273"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF22">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 11A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="1" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="243" mass="27161" checksum="B7EDB65D9D57F790" modified="2018-12-05" version="1">MDAVVKFTSQSQGRDRIFRSTQYACALSIYLLRNKPDRKDLVARLKSLENNLSAGRKLLRLGNAANSIVAAKQTMQLSDRVLGLCLTVANINRALYFICDNVVWARNVGLIRSIDKERWSINASRYYLFSLVMNLTRDLYVILQLMQQKGRDNRLKSQHLSDCPEVAEAVIPELDALIFLLLETLRSEPTVALDTVKNICDLFIPLDTLGIYKSSAGAVGFCGLISSLIGIITLAQPTLRIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZP8</accession>
    <name>A0A3B5MZP8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">NADH-cytochrome b5 reductase</fullName>
            <ecNumber evidence="15">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14 15">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MZP8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027727"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028062.1">
        <property type="protein sequence ID" value="ENSXCOP00000027727.1"/>
        <property type="gene ID" value="ENSXCOG00000020676.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NADH"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:FAD binding"/>
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        <property type="term" value="P:response to yeast"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:sterol biosynthetic process"/>
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        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
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        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CBR-like"/>
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        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
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        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
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        <property type="entry name" value="FNR_nucleotide-bd"/>
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    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
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        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF108">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
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        <property type="entry name" value="FPNCR"/>
    </dbReference>
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        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Riboflavin synthase domain-like"/>
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    </dbReference>
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        <property type="entry name" value="FAD_FR"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14 15" id="KW-0274">FAD</keyword>
    <keyword evidence="4 14 15" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 15" id="KW-0520">NAD</keyword>
    <keyword evidence="7 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="11" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="17">
        <location>
            <begin position="46"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
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        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>FAD</name>
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        </ligand>
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        <location>
            <position position="115"/>
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        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
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        <location>
            <position position="117"/>
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        <ligand>
            <name>FAD</name>
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        </ligand>
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        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="307" mass="34159" checksum="C1171BEA2620B1B9" modified="2018-12-05" version="1">MLHMTRLNTIPVVLAVSVVVITMLFFLLGNSADGKGKGPVTLKDPTIKYSLPLISKQKISHDTKKFRFGLPSESHILGLPVGQHVYLSAKVKGNLVVKPYTPVSSDEDQGFVDLVVKIYYKGTHPNYPDGGQMSQYLDNMAIGDSIDFRGPNGLLIYKGKSMFSIQANKKTEPVVQKFKHVGMIAGGTGITPMLQLIRRITSDPSDNTKCSLIFANQTEKDILLWDELKDVQKNHPEKVKVWYTLDKPSKDWNYSSGYVTLEMIKDHIPAASRDVLIVLCGPAPMIRNACLPNLEKLGHTKENIFAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N0P5</accession>
    <name>A0A3B5N0P5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Poly [ADP-ribose] polymerase</fullName>
            <shortName evidence="17">PARP</shortName>
            <ecNumber evidence="17">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="17"/>
    <dbReference type="RefSeq" id="XP_027859222.1">
        <property type="nucleotide sequence ID" value="XM_028003421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5N0P5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026795"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027124.1">
        <property type="protein sequence ID" value="ENSXCOP00000026795.1"/>
        <property type="gene ID" value="ENSXCOG00000020013.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114135817"/>
    <dbReference type="KEGG" id="xco:114135817"/>
    <dbReference type="CTD" id="10039"/>
    <dbReference type="GeneTree" id="ENSGT00940000158855"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990404">
        <property type="term" value="F:NAD+-protein mono-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070212">
        <property type="term" value="P:protein poly-ADP-ribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01437">
        <property type="entry name" value="parp_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08002">
        <property type="entry name" value="WGR_PARP3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.142.10:FF:000002">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.140.10:FF:000001">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.228.10:FF:000009">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.142.10">
        <property type="entry name" value="Poly(ADP-ribose) polymerase, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.140.10">
        <property type="entry name" value="WGR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050800">
        <property type="entry name" value="ARTD/PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004102">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036616">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036930">
        <property type="entry name" value="WGR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008893">
        <property type="entry name" value="WGR_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459:SF66">
        <property type="entry name" value="PROTEIN MONO-ADP-RIBOSYLTRANSFERASE PARP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02877">
        <property type="entry name" value="PARP_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05406">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00773">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47587">
        <property type="entry name" value="Domain of poly(ADP-ribose) polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142921">
        <property type="entry name" value="WGR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51060">
        <property type="entry name" value="PARP_ALPHA_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51977">
        <property type="entry name" value="WGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 17" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 17" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="WGR" evidence="21">
        <location>
            <begin position="55"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="PARP alpha-helical" evidence="20">
        <location>
            <begin position="173"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="PARP catalytic" evidence="19">
        <location>
            <begin position="305"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="522" mass="58945" checksum="08B0EA98F12C68E5" modified="2018-12-05" version="1">MAPKRKAAKAVKAGGKKVKDEPKDAFTSTKEALLAAGSQVKAKRKVDEHCELFSSGEIHEDYDCMLNQTNIGNNNNKFYVIQVIKTGNSYYSWNRWGRVGEVGQSKLTQFPRPELALKDFERKFKDKTKNNWSDRMNFVSHPGKYTLIEVDGEQDAEVKVDTVDGKVSKNFLPCTLDGATQNLIKLIFSNDMFKEAMKCMNLDIKKMPLGKLSKGQIAKGFEVLEEIEAAMSQKDGRGRLEELSSKFFTAIPHNFGRNRPPAIDTKEIVEQKKEMLMVLADIELAQTLKSETEKAQDEMVETVPHPIDQDYMSLKCELSLLAKNTEMYKIIEKYMKSTTCTYSKPKILNVWEVDREKEGERFSENDSLENRRLLWHGTNIAVVAAILKSGLRIMPHSGGRVGSGIYFASENAKSIGYVRPSNNTGVMFLSEVALGKEFTITRDDCTLKKAPAGYHSVVARGLLEPDPSQDTIITLEGKKVTVPQGKAIEQPQYANSSFSNSEYLIYKESQCRLRYLLEMEMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N179</accession>
    <name>A0A3B5N179_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N179"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027285"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027618.1">
        <property type="protein sequence ID" value="ENSXCOP00000027285.1"/>
        <property type="gene ID" value="ENSXCOG00000020375.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF27">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="138" mass="15404" checksum="7A51732E630B8897" modified="2018-12-05" version="1">GDVEVVGSGLEDGILTAGFYFGFGFHHLAGVHVSGKAWITPKGFDTGIKTFNSLTKQKEALILAREGTLWTYMDLNPLLPPSSYVRFDILQRILTRVFGVTVIHAMVITDIDDKIIRRSWEVWITFMCPTQGDCRAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KMP6</accession>
    <name>A0A3B5KMP6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Ankyrin repeat and zinc finger peptidyl tRNA hydrolase 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The VLRF1 domain mediates binding to the 60S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ANKZF1/VMS1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KMP6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001373"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001396.1">
        <property type="protein sequence ID" value="ENSXCOP00000001373.1"/>
        <property type="gene ID" value="ENSXCOG00000001114.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005911"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047139">
        <property type="entry name" value="ANKZ1/VMS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041540">
        <property type="entry name" value="VATC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041175">
        <property type="entry name" value="VLRF1/Vms1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16036">
        <property type="entry name" value="ANKYRIN REPEAT AND ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16036:SF2">
        <property type="entry name" value="TRNA ENDONUCLEASE ANKZF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18826">
        <property type="entry name" value="bVLRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18716">
        <property type="entry name" value="VATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52044">
        <property type="entry name" value="VLRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 14" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="VLRF1" evidence="16">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="13">
        <location>
            <begin position="269"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="329"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="188"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="350"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="364"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS52044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="444" mass="50451" checksum="CB697E9F3E0BD294" modified="2018-12-05" version="1">MTGGGHFAGAVFEGKQVLDHKTFHRYTVRAKRGTAQGLRDSQNRSHMARSAGATLRRYNEAALVKDIQDLLLTWAEHLNRASAVFVRAPSYNKAIFFGGRGGALQKNDLRVRSLPIATRRATFREVQRVHEVLSTVQVYGVFKYMFRAVACWEENNESSDEEGEEIQLEMVELTIGTLDLREHEMHPCRRRKRRRKKKEQGKLHNQEAVDESVDYGLRDALFTACKVGDVGALCSLLQLPQETMDHPEKMEENISTAPLSLLNKPIDSSGFSLLHVAAAAAQKAVVRLLLHAGADPACRDNKGQTPYTVAPDKDTRNVFRKYMGENPDKYDYSKAQVPGPLTAETESKKVEKKKAQRAQRKHREKEQKEEKRKQELEAEEKKKFASLTDREKVRKRAIKLESQRCWSCGESLLGKIPFNYLEYTFCTPRCVQAHRKANAPPNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KPM2</accession>
    <name>A0A3B5KPM2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tubulin delta chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Delta-tubulin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Acts as a positive regulator of hedgehog signaling and regulates ciliary function.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027855561.1">
        <property type="nucleotide sequence ID" value="XM_027999760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KPM2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000412"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000420.1">
        <property type="protein sequence ID" value="ENSXCOP00000000412.1"/>
        <property type="gene ID" value="ENSXCOG00000000360.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114133681"/>
    <dbReference type="KEGG" id="xco:114133681"/>
    <dbReference type="CTD" id="51174"/>
    <dbReference type="GeneTree" id="ENSGT00940000157069"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02189">
        <property type="entry name" value="delta_zeta_tubulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000021">
        <property type="entry name" value="Tubulin delta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002967">
        <property type="entry name" value="Delta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01224">
        <property type="entry name" value="DELTATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 16" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7 16" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="17">
        <location>
            <begin position="40"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="444" mass="49221" checksum="2B4FEEB0ED23A2C2" modified="2018-12-05" version="1">MSVVTVQLGQCGNQVGQELFDVICSDARDVHRKTYSTVSCERFFRETTQGDLEARAVLIDMEPKVINHSMSKAAKSGRWRYGETSHFSQKQGSGNNWANGYCVHGPRHRDAVEELMRREVEHCDMLAGFIPIMSVAGGTGSGVGTFVTQCLRDAYPSSFVLNHLTWPYGTGEVIVQNYNSVLTLAHLYQLSDAILVHENDIVHKICSQLLKIKQISFSDINRVIAHQLGGVLQPTLTPASCGAYSRHPIGELMSALACHPEYKLLSVCTIPQMASSSIPFSTFSWPALLKHLRQMLISNSKMEGGIDWQVCPPPASAGRSRSLSAGSFNTSLANLLILRGKDVYSAETGGFEDSAIYTSWLPPDEALSLWKSPVPFNKYEKSATLVSNSQALLRPLDDMVRKAWNMFASRAYIHQYVKYGISEEDFLDSFTSLEQVISSYSELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KPS8</accession>
    <name>A0A3B5KPS8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NSL1 component of MIS12 kinetochore complex</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KPS8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000467"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000475.1">
        <property type="protein sequence ID" value="ENSXCOP00000000467.1"/>
        <property type="gene ID" value="ENSXCOG00000000412.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001374"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000444">
        <property type="term" value="C:MIS12/MIND type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013950">
        <property type="entry name" value="Mis14/Nsl1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31749">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN NSL1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31749:SF3">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN NSL1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08641">
        <property type="entry name" value="Mis14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="226" mass="25180" checksum="56B454A03A533982" modified="2018-12-05" version="1">MESFPIQTSRHEDPEQEFRVRLTSKRKTTELMNRCCELLRSALDGQPDISEDSRRVLVQELLNFEAAVRLNVLVDGQPWDEAPDLEGTSYEEALCLDSLLDDSILDTAWRRRSYPKRILVHVVHALKAERKVLVGSVQPTSLTVRLSLQSIDALQQQATGLCEILNLKPSAASLQIHREVLGSTAMPPAGGAARGRQPVHRAVEEAAAQGYEADSRGAERSEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTA0</accession>
    <name>A0A3B5KTA0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTA0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001753.1">
        <property type="protein sequence ID" value="ENSXCOP00000001727.1"/>
        <property type="gene ID" value="ENSXCOG00000001397.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00960000186715"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="169" mass="19628" checksum="77CDCF7ADE454A92" modified="2018-12-05" version="1">MDRLLLSVQRLLQHQLREFGPVALRLHVEGKIVIVGYSIRAERVGANVGVEGVLHGETSPGYGTLRYFHENVRLRKTGRIVVDIHHLDLDSEQLQRILQKHLQMQQARDGLLADFFPIYFLFHNQSAVLQVHLQIRCPRTWHRLEPIFCDIPNYGARLLLFWHGVVQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KWE2</accession>
    <name>A0A3B5KWE2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the myelin P0 protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KWE2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002340"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002374.1">
        <property type="protein sequence ID" value="ENSXCOP00000002340.1"/>
        <property type="gene ID" value="ENSXCOG00000001874.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234556"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000193">
        <property type="entry name" value="Myelin protein zero-like 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000920">
        <property type="entry name" value="Myelin_P0-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13869">
        <property type="entry name" value="MYELIN P0 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13869:SF21">
        <property type="entry name" value="MYELIN PROTEIN ZERO-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="14"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="208" mass="23367" checksum="EBC0463F3ACAD420" modified="2018-12-05" version="1">MLFSFECKHFTTCPFAGVLQVSGMRVYTSGDVEAVNGTDVRLKCTFDSSAKINPDLVIISWTFKPLSGGRVDRVSSYVCIFHYQQKPYPPLDGIFKKRISWAGDIMGSDASIILREVKFIYNGTYTCQVTNPPDVHGPVGEIRLRVVKTASFNELLYLVLAIGGGIAAVVFCLIVIVSCRRCKRKHRQRQLEGDEEVPRKERKDPTAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KWQ5</accession>
    <name>A0A3B5KWQ5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Serpin peptidase inhibitor, clade D (heparin cofactor), member 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027889470.1">
        <property type="nucleotide sequence ID" value="XM_028033669.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027889471.1">
        <property type="nucleotide sequence ID" value="XM_028033670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KWQ5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002061"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002091.1">
        <property type="protein sequence ID" value="ENSXCOP00000002061.1"/>
        <property type="gene ID" value="ENSXCOG00000001600.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114154528"/>
    <dbReference type="KEGG" id="xco:114154528"/>
    <dbReference type="CTD" id="3053"/>
    <dbReference type="GeneTree" id="ENSGT00940000158664"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02047">
        <property type="entry name" value="serpinD1_HCF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.39.10:FF:000002">
        <property type="entry name" value="Serpin family D member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033831">
        <property type="entry name" value="HCII_serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF30">
        <property type="entry name" value="HEPARIN COFACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00780">
        <property type="entry name" value="LEUSERPINII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017246740" evidence="3">
        <location>
            <begin position="20"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="4">
        <location>
            <begin position="141"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="505" mass="57913" checksum="39183CDDCC55E3DF" modified="2018-12-05" version="1">MWVFTVISVVCMLASPSLAGIKELSSHFNDPNPNPRGLQPGSPVDVEAIPLDFHKENTVTNDLVFDGFEDEDYIDFDKILAAGSDDYIEGDEIDEIATPAPDIDVFAESSDPKIRRARLLRLFHGHSRLQRLNMVNARFGFNLYRSIRNDVNQTDNILLAPAGISIAMGMMSLGAGSETHHQIYKALGFAEFVNASHHYDNTTVHKLFRKLTHRLFRRNFGYTLRSVNDVYLKKNVSVKETFRTETKAYYFAEPQSVDFREPAFLNKANRRIEKLTKGLIKEPLKSMDPNMVLMLLNYLYFKGTWEQKFPKEMTYYRNFRVNDKTNVRVPMMMNKGNYLAAADHELECDILQLPYTGNISMLIALPRKITGMRTLEQQISPTVVNKWLKNMTNRTREVSLPRFKLEQNYDLMENLKEMGLTDIFKESGDFSGMTSEKVALNWLKHQGTITVNEEGTEAAALTQVGFMPLSSQIRFTVDHPFLFLIYEHRTDCLVFIGRVVNPSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2B4</accession>
    <name>A0A3B5L2B4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Growth hormone releasing hormone receptor b</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2B4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006453"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006533.1">
        <property type="protein sequence ID" value="ENSXCOP00000006453.1"/>
        <property type="gene ID" value="ENSXCOG00000005000.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166873"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017046">
        <property type="term" value="F:peptide hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004999">
        <property type="term" value="F:vasoactive intestinal polypeptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15271">
        <property type="entry name" value="7tmB1_GHRHR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000032">
        <property type="entry name" value="Vasoactive intestinal polypeptide receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050332">
        <property type="entry name" value="GPCR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001771">
        <property type="entry name" value="GPCR_2_VIP_rcpt_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF6">
        <property type="entry name" value="GROWTH HORMONE-RELEASING HORMONE-LIKE PEPTIDE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01154">
        <property type="entry name" value="VIP1RECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111418">
        <property type="entry name" value="Hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="264"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="14">
        <location>
            <begin position="38"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="15">
        <location>
            <begin position="112"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="405"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="411"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="429" mass="49601" checksum="5DC99C24C60AD9B6" modified="2018-12-05" version="1">MRTSWQHAYNFIKCVVPEDIGAVTLVNVYYILIVFPGCPTYWDDIYCWHRANVGQVVRVSCTNVSQLFADNQAYISRNCTEEGWYEHLNYHEACVFKDVEEPEPETTYFSNFKQVYTAGYATSLISLISAIFVFTVFRKFHCTRNYIHINLFFSFILRATAVFIKDLVLFSDEDLDHCFMSTVTCKSAVAFFQFSILANYFWLLVEGMYLQTLLALTFVFQKKYFWWYILIGWGLPAIIIATWILSRNFYNNKGCWDDTDVAFIWWIIKGPITASLLVNFIIFLNVIRILVQKLTSPGVGGNNTSHFKRLAKSTLLLIPLFGMHYMVFAFLPENTGAEARIIIEVGMGSFQGFVVALLYCFLNGEVQAELKKRFWKWQTESYLSYSKRRRTLFNESSTVTQISVLDKSSPKEQQNSESHALTPNNVSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4V3</accession>
    <name>A0A3B5L4V3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L4V3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002789"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002826.1">
        <property type="protein sequence ID" value="ENSXCOP00000002789.1"/>
        <property type="gene ID" value="ENSXCOG00000002219.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157087"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="13752" checksum="27CEF88908A53E1D" modified="2018-12-05" version="1">NKLRERERERNNVPKHEIYGRVESVKVLPKRGSEGGVAAFVDFVDIKSAQKAHNAINKMGDRDLRTDYNEPGTIPSAARGLDDSLSLGSRGRDVSGFTRATGGAVYGPPTSLHSRDGRYERRLDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L508</accession>
    <name>A0A3B5L508_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NACHT LRR and PYD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L508"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004971.1">
        <property type="protein sequence ID" value="ENSXCOP00000004911.1"/>
        <property type="gene ID" value="ENSXCOG00000003852.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286915"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000100">
        <property type="entry name" value="Si:dkey-11n14.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT LRR and PYD" evidence="3">
        <location>
            <begin position="10"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="416" mass="45420" checksum="FE85850409D98C5A" modified="2018-12-05" version="1">MKETQTSKKFLLFNEPKIISVHQRAVDQALQSPNGHLDLFLRFLLGLSLQTNQRLLQGLLTKTGSSSQTNQKTVQYIKKKISKNVSAEKSINLFHCLNELNDRSLVEEIQLSLSSGSLSTDKLSPAQWSALGFILVSQGKDLDVFDLKKYSASEEVLLRLLPVVKNYLIFSPLSLRLSVCNLSERSCEALSSVLSSQSSSLRELDLSINNLQDSGVKLLSAGLKSPNCNLETLNLSGCLVSEEGCASLASALTSNPSHLKELDLSYNHPGDSGVKLLSAGLMDPYWRLEALRYGGTCCRTLSSFISSTTSSLKDLDLSNNDLTDSGVKLLCEGLMSPNCELKILRLSGCLITEEGCAALASALTVNSSHLKELDLSYNNPGEAGMKRNIFYASKVKPESKFLPMFLPDLQILQVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5H5</accession>
    <name>A0A3B5L5H5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">PHD finger protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Polycomblike family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5H5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005655"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005721.1">
        <property type="protein sequence ID" value="ENSXCOP00000005655.1"/>
        <property type="gene ID" value="ENSXCOG00000004398.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183180"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045814">
        <property type="term" value="P:negative regulation of gene expression, epigenetic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20449">
        <property type="entry name" value="Tudor_PHF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000086">
        <property type="entry name" value="PHD finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000125">
        <property type="entry name" value="Putative PHD finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.980.20:FF:000003">
        <property type="entry name" value="Putative PHD finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040477">
        <property type="entry name" value="KDM4-like_Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025894">
        <property type="entry name" value="Mtf2_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047399">
        <property type="entry name" value="Tudor_PHF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12628:SF11">
        <property type="entry name" value="PHD FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12628">
        <property type="entry name" value="POLYCOMB-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14061">
        <property type="entry name" value="Mtf2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18104">
        <property type="entry name" value="Tudor_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="11">
        <location>
            <begin position="93"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="357"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="412" mass="45798" checksum="4954B32BA67D95FC" modified="2018-12-05" version="1">MHTSHFLQTSTLRIGAGSNWTSTPCSPLSVSLLRMGGVSEGDDVLARWSDGLLYLGNVKRVDGVKQCCLVRFEDNSEFWVLRKDIHSFAAGVEEVCCICDAPPLKEPLINCLKCHHGYHPECHTPTIEPEADSDSWICRQCVFAVATKRGGALKRGRFARLMQFMKLRIPYQLSSLDWDPQHLTNQQQCYCYCAGPGEWNLKMLQCGSCGQWFHEACTQCLLKPLLYGDRFYQFQCSVCTKGPETVQRLPMTWVDLAHLVLYHLSLCCKRKYFDFDHEILSFTNENWDSLLLGALSDTPRQDRCHNLLNALNSHKDRFVSGKEIKKKKCLFGLQVRAPPPLTSDSSPLVTDPPISSGGAGSGAGMGVGGGGGDGTVVRSWAGGGEAVRILARRVTPDGKVQYLVEWDNVGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5P5</accession>
    <name>A0A3B5L5P5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Inositol 1,4,5-trisphosphate receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 19">Endoplasmic reticulum membrane</location>
            <topology evidence="1 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="19">The ITPR1 structure has a large solenoid CY assembly built around the central helical bundle made of the C-terminal domains from four ITPR1 subunits. The solenoid scaffold includes domains responsible for binding of ligands and regulatory proteins and is connected via an allosteric nexus at the cytosolic-membrane interface to the transmembrane channel assembly. Six transmembrane helices from each subunit form the central ion-conduction pore.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the InsP3 receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5P5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006137"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006207.1">
        <property type="protein sequence ID" value="ENSXCOP00000006137.1"/>
        <property type="gene ID" value="ENSXCOG00000004735.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155071"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070679">
        <property type="term" value="F:inositol 1,4,5 trisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005220">
        <property type="term" value="F:inositol 1,4,5-trisphosphate-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000002">
        <property type="entry name" value="Inositol 1,4,5-trisphosphate receptor type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.30:FF:000001">
        <property type="entry name" value="Inositol 1,4,5-trisphosphate receptor, type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.30">
        <property type="entry name" value="IP3 receptor type 1 binding core, RIH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014821">
        <property type="entry name" value="Ins145_P3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000493">
        <property type="entry name" value="InsP3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000699">
        <property type="entry name" value="RIH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015925">
        <property type="entry name" value="Ryanodine_IP3_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035910">
        <property type="entry name" value="RyR/IP3R_RIH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816:SF2">
        <property type="entry name" value="INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45816">
        <property type="entry name" value="MIR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08709">
        <property type="entry name" value="Ins145_P3_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01365">
        <property type="entry name" value="RYDR_ITPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00779">
        <property type="entry name" value="INSP3RECEPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100909">
        <property type="entry name" value="IP3 receptor type 1 binding core, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 19" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="5 19" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="17" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9 19" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16 19" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 19" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 19" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 19" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 19" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MIR" evidence="20">
        <location>
            <begin position="60"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="20">
        <location>
            <begin position="179"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="20">
        <location>
            <begin position="327"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU368044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="993" mass="113237" checksum="0B68780FE40AB7E3" modified="2018-12-05" version="1">MHKPAQFLCPMNRYSAQKQFWKAAKPGGNSTTDTVLLNKLHHAADLEKKQNESENRKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDSAGNEGSWFYIQPFYKLRSLGDSVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCNTSWKVVLFMKWSDNLETILKGGDVARLFHAEQEKFLTCDDHRKKQYVFLRTTGRQSATSATSSKALWEVEVVHHDPCRGGAGYWNSLFRFKHLATGCYLAAEVNPDFEEESAEQRSSLDADNEALRGRLRAPQEKIMYTLVPVPDGMEVSSIFELDPTTLRGGDSMVPRSSYVRLRHLCTNTWVHSTNLPIDKEEEKPVMLRIGTSAVKEDKEAFAIVPVPPSEVRDLDFANDASKVLASIAGKLEKGTITQNERRFVTKLLEDLVFFVVDIPNSGQDVLEIMVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELGDQRHAPFRHICRLCYRVLRHSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVTLVRKNREPRFLDYLSDLCVSMHKSIPVTQELICNAVLDSSNADILIETKLVLSRFEIESTANGEDPVEAEEVEEVWLFWKDNCKEVHSKSVRELAQDAKEGQKEDQEVVSYYRCQLNLFARMCLDRQYLAINKISGQLDVDLILRCMSDEDLPFDLRASFCRLMLHMHVDRDPQEQVTPVKYARLWSEIPSKIAIDDYDNDGTTRDEIKERFSLTMDFVETYLREVVSQNIPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLNILDCVHVSTVFPINKIEKGDESKGGSNVMKSIHGVGELMTQVVLRGGGLLPTTPTHQPEGDVVKTQTEPEREDIMVMDTKLKIIEILQFILNVRLDYRISCLLCIFKREFDESNPQGDNAVSANGGNNITTQMPGNLDFENIEEQAEGIFGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6R7</accession>
    <name>A0A3B5L6R7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="20">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Membrane</location>
            <topology evidence="20">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Sarcoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6R7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006135"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006205.1">
        <property type="protein sequence ID" value="ENSXCOP00000006135.1"/>
        <property type="gene ID" value="ENSXCOG00000004732.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159895"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02083">
        <property type="entry name" value="P-type_ATPase_SERCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000143">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000003">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000005">
        <property type="entry name" value="Calcium-transporting ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000065">
        <property type="entry name" value="Sarcoplasmic/endoplasmic reticulum calcium ATPase 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005782">
        <property type="entry name" value="P-type_ATPase_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01116">
        <property type="entry name" value="ATPase-IIA1_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 20" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 20" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="18 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="19 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="16" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 20" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="292"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="752"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="826"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="923"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="962"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1000"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="21">
        <location>
            <begin position="3"/>
            <end position="71"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004326"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005675"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022568"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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            <dbReference type="ARBA" id="ARBA00022951"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006135.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1036" mass="114130" checksum="12BFBCDD74F19BA3" modified="2018-12-05" version="1">MDNAHTKTVEEVFSFFNVNESTGLSLEEVKKQRERFGPNGKSLWELVIEQFEDLLVRILLLAACISFVLAWFEEGEETVTAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRQDRKSVQRIKARDIVPGDIVEVAVGDKVPADIRLTSIKSTTLRVDQSILTGESVSVIKHTDPVPDPRAVNQDKKNMLFSGTNIAAGKAVGVVVATAGNTEIGKIRDEMASTEQERTPLQQKLDEFGQQLSKVISLICIAVWIINIGHFNDPVHGGSWIRGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFIIDKTEGDHCPLKEFTVTGSTYAPDGEVYHNGKPVKCSHYDALVELATICALCNDSSLDYNETKGVYEKVGEATETALTCLVEKMNVFDTDIKNLSTVERANACNSVIKQLMKKEFTLEFSRDRKSMSVYCTPNKARSSVGKMFVKGAPEGVIDRCTHIRVGSNKVPMTPGIKEKIMSVIREYGTGRDTLRCLALATRDNPVNKENLVLEDSSRFVEYETDLTFVGCVGMLDPPRAEVAASIRLCRLAGIRVIMITGDNKGTAVAICRRIGIFGEEDDVSSMAFTGREFDDLSPAAQRDAVVKARCFARVEPAHKSKIVEYLQSYDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNMKQFIRYLISSNVGEVVCIFLTAALGFPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMSKPPRNAREPLISGWLFFRYLAIGCYVGAATVGAAAWWFVAAEDGPRITFYQLSHFLQCGPENPDYQGLDCKVFESPYPMTMALSVLVTIEMCNALNSVSENQSLLRMPPWENVWLLGAICLSMSLHFLILYVEPLPIIFQITPLNLTQWLMVLKISLPVILLDELLKFAARNYLERNRELEKLPSSKGCCLFACMEGISWPFVAISLPLVLWIYSSDTNLADMFMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7Z7</accession>
    <name>A0A3B5L7Z7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L7Z7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006942"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007030.1">
        <property type="protein sequence ID" value="ENSXCOP00000006942.1"/>
        <property type="gene ID" value="ENSXCOG00000005367.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158699"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
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    </dbReference>
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        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="1">
        <location>
            <begin position="16"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="146" mass="16452" checksum="2987634B4E9C9FC7" modified="2018-12-05" version="1">MDLGFPKDSTLWKGLPLQLDDAMRWSDGSSYFFKGKEYWRVPGSDMEAEAGYPRLIAKDWLLCTEMQSDSPDTEAKSTETRVNVHRHPDHAENGYEVCSCTSDTASPLGVHLSPSPVWLLLPFWTLLLTFFCGAFLTQAQAPFDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LB93</accession>
    <name>A0A3B5LB93_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Nucleolar and spindle associated protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NUSAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LB93"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005144"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005205.1">
        <property type="protein sequence ID" value="ENSXCOP00000005144.1"/>
        <property type="gene ID" value="ENSXCOG00000004029.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006370"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040001">
        <property type="term" value="P:establishment of mitotic spindle localization"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="NuSAP"/>
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    <dbReference type="PANTHER" id="PTHR15874">
        <property type="entry name" value="NUCLEOLAR AND SPINDLE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15874:SF1">
        <property type="entry name" value="NUCLEOLAR AND SPINDLE-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16006">
        <property type="entry name" value="NUSAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="39"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="270"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="280"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="406" mass="44734" checksum="A4A96B3F22DE5901" modified="2018-12-05" version="1">MKMDLNSMKYAELRSIAKELGLKANTKADKLLKAIKQHYEKEKKNDEGDENAASDSGREDADDASQQEAPSTAGFVNTRRGRGKCIKRKITDAAEVLGCNSDLSKALLKPTTPSKFSPPLTRCSCGRMRSDDPFEPFKVTVVNLISKPDFKKLHEAQFNKMESIDSYVQRKTKQVETYKNPDLFGSVDRCVCVLQKNSARATMFSPVNKTPAEENLRHTLKASKIPSKKPAQKEDGQFRPSVLSARRVNVRFSEATLDNEFKRSLVKTPARMSPCVASSTPQKPTADAAKPAAAKSSALSAQKTPGPFIFTGNTSALGTPGTQKKPAFDLKASLSRPLSYQPHKGKLKPFGDVKENAAGNKSSPSVQLLLMTICPVLRKDRRTKQVESRKQKKENMLGARRGLVMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBX8</accession>
    <name>A0A3B5LBX8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Caspase 7, apoptosis-related cysteine peptidase</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase C14A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBX8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008347"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008452.1">
        <property type="protein sequence ID" value="ENSXCOP00000008347.1"/>
        <property type="gene ID" value="ENSXCOG00000006396.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153232"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097194">
        <property type="term" value="P:execution phase of apoptosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043525">
        <property type="term" value="P:positive regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00032">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000001">
        <property type="entry name" value="Caspase-3 preproprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033139">
        <property type="entry name" value="Caspase_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016129">
        <property type="entry name" value="Caspase_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002398">
        <property type="entry name" value="Pept_C14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002138">
        <property type="entry name" value="Pept_C14_p10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10454">
        <property type="entry name" value="CASPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10454:SF31">
        <property type="entry name" value="CASPASE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00376">
        <property type="entry name" value="IL1BCENZYME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01122">
        <property type="entry name" value="CASPASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01121">
        <property type="entry name" value="CASPASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50207">
        <property type="entry name" value="CASPASE_P10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Caspase family p20" evidence="10">
        <location>
            <begin position="64"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p10" evidence="9">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="285" mass="32242" checksum="0D0DDF0E868BBC6F" modified="2018-12-05" version="1">MSTPTRRLSPWATPEWKYAQPLSRRLVPETDKKNKDGQTREQDYSKSHSRIVFPTFQYKMSYRQVGKCIIINNKNFDENTGMNVRNGTDRDAGELFKCFRSLGFEVVVYNDQTCEKMEHLLKQASEEDHSDSSCFACILLSHGEEGMIYGTDAAMPIKTITSLFRGDRCKSLVGKPKLFFIQACRGSEFDEGVQTDSGPPNDTLETDANPRHKIPVEADFLFAYSTVPGNMITLKQSKHTLSVRFSQVSLGTTPKVCCFFTTMTNTPLPVRSLFSQSDYLSVSMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LC22</accession>
    <name>A0A3B5LC22_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LC22"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005419"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005481.1">
        <property type="protein sequence ID" value="ENSXCOP00000005419.1"/>
        <property type="gene ID" value="ENSXCOG00000004235.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000170682"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF7">
        <property type="entry name" value="INTERLEUKIN-21 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017370451" description="Fibronectin type-III domain-containing protein" evidence="8">
        <location>
            <begin position="18"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="476" mass="53154" checksum="4FF30D670EECB2A5" modified="2018-12-05" version="1">LNVQPLQLTSFFSSAAAASVCNVTCTTDYVKMLNCSCQGSVPTLPVRLTVACSSEDTHLTVSCETEPPQSWCIIKTDLTYVASTETLCNATASQPGGRGPVVLSESHQWELYSVVKLEAPHNVTVKKAAGIYNIAWRNKSEYTSCVINKDLIFKLLPNRMYFELDQKKLRPGASYVVNVQAKICDDEFIVGPWSEWSSDAEWSSPTNINSTKLSWQKKLKMMTFIPRPNDYFKPLYHSHGGNFKEWVKPVFNEYDYLKITTCVMLSEKQSEILNWSNEKQSYSEDSSTSQGTIHSTGHISIHTVTLSGEEFEADVTSQSSLKSFQDAESFGSFDDDNRERVAYNLGESHASRMERHSGISLRENQIANDLILENQNFLPDGEFNEAERVSLNSFASNEQSEDGYPHVDLDTIDSGFGECGSPGASDSNSNSFPEEKHFNSNYVKQWMICSTIQEDTSTVNNELDETVTAASDSQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LD38</accession>
    <name>A0A3B5LD38_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LD38"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010093"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010212.1">
        <property type="protein sequence ID" value="ENSXCOP00000010093.1"/>
        <property type="gene ID" value="ENSXCOG00000007652.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157142"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04391">
        <property type="entry name" value="RhoGAP_ARHGAP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000018">
        <property type="entry name" value="Rho GTPase activating protein 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057323">
        <property type="entry name" value="RHG40/28/18_ubiquitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14963">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN 18,19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14963:SF6">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25442">
        <property type="entry name" value="Ubiquitin_RHG40_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="5">
        <location>
            <begin position="306"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="69"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="88"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00055252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="635" mass="72629" checksum="79DC06714868BC05" modified="2018-12-05" version="1">MFARSLAIHIIYFFFLISFFLFLCVINSRKTGHYTVNVHIPVPECLHFPQDSLDELEMDDYWTEIENIKSSGEGHERGGGEGETQEEEQQKAPEEGEHEEAWLTEAGLVRLVDDSLAEEDSAVFLSTLTRTQAAAVQLRVSSYQQTLLRRRNRQHFPDVRDIFRPPEKNEEPPKLKEESDKDATTVGMSTTCWAYYFISPYFSAETETELNVEVAFSEQALSYRDNNQRLKVNSSLNSSDDKLPNFKLLRDKTGQTRVGDLSPLDMKKVHRLVLVEMTALFDTAGIDVKANKPGRVKSKESGLFGVPLITLLDQDHRRVSGTKVPLILQKLISHVEEEGLDTEGLLRIPGSATRVKSLCQELESSFYDGTFQWKQVKQHDAASLLKLFIRELPHPLLTVEYLNAFIAVNKLPTKKQQLQALNLLVLLLPEANRDTLKALVEFFQRVINHQVKNKMTLNNVSVVMAPNIFMFKGFRSKVTEQQEFSMATSTANIVRLLIRYQNLLWTIPKFILTQVRQQNMENQRKLYKDRAVRKLLKKITTDRPIDKNTQGIIRVQAPQFSKISMAIQLTEDLQAADVLTRFLSQDSSVSVKKEDLCLYEIGGNIKERCLAGETYMKDLSQLNPAAEWVIKEVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEN9</accession>
    <name>A0A3B5LEN9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Apoptosis inducing factor mitochondria associated 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LEN9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009307"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009418.1">
        <property type="protein sequence ID" value="ENSXCOP00000009307.1"/>
        <property type="gene ID" value="ENSXCOG00000007089.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166421"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03478">
        <property type="entry name" value="Rieske_AIFL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050446">
        <property type="entry name" value="FAD-oxidoreductase/Apoptosis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557:SF9">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="9">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="492" mass="54533" checksum="34526DE4D86FB8B3" modified="2018-12-05" version="1">MMEVDIGRHSVLLTRCDGKYSAISNQCSHYGAPLSKGVISGHKVRCPWHGSCFNALTGDLEEFPGIDSLPCHKVKIQNSKVYVSINKKDIRHQKRLQRMGAAVPGVNHTVLLLGGGAASLICAETLRQENFGGRIIMASRDDLLPYDKTRLSKVMNVLSDTIVLRRMEFFHQYDIEVWLRKEAVSVDTEKKTVTFDDGLVQSYDQLLLSTGCAKGLDVPGRKLDNIKMLETPEDARQIHAASLGCNIVLVGTSFVMEVASYMIDKASSITVVGSSDMPYQNTLGLEVGKITMMMLLEKGVRFYMNDHVIEIKGVNGKVKEIVLKSGHVIPADVLIVGIGSIPNSEFLQGTKIQKNSKNYVVVDKYMRTNVPDVFCAGDLASFPLALAKNQLVNIGHWQMAQVQRIAALNMLKKTTELTSVPYYWTILQGRTIRYAYGEGYTEIVMKGNYEFEERKFLANDEVIAAVGLDYDPVVSAVAERLLSGKVITKKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEW3</accession>
    <name>A0A3B5LEW3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">XK-related protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Phospholipid scramblase that promotes phosphatidylserine exposure on apoptotic cell surface. Phosphatidylserine is a specific marker only present at the surface of apoptotic cells and acts as a specific signal for engulfment.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the XK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LEW3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009115"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009226.1">
        <property type="protein sequence ID" value="ENSXCOP00000009115.1"/>
        <property type="gene ID" value="ENSXCOG00000006953.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282533"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902742">
        <property type="term" value="P:apoptotic process involved in development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043652">
        <property type="term" value="P:engulfment of apoptotic cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070782">
        <property type="term" value="P:phosphatidylserine exposure on apoptotic cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018629">
        <property type="entry name" value="XK-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050895">
        <property type="entry name" value="XK-related_scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16024">
        <property type="entry name" value="XK-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16024:SF16">
        <property type="entry name" value="XK-RELATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09815">
        <property type="entry name" value="XK-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="166"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU910716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="233" mass="25730" checksum="859BF427C03CE4FC" modified="2018-12-05" version="1">MAAKSDGVLKLNRSDVAFTPLQNSEHSGSVQGLHLGQQTDSACTLDVDFPNGESQCCGRGGSNSPCFRLSREQQKYVIWDCLWIVAAVSVYLADVGTDVWLSVDYYLRQDYWWFGLTLFFVVLGSFSVQLFSFRWFVADFSTEDNVDSAVGCPQLDGSKLLLSGSASQGDVPVQQHPATPQRQMSTASKNTATNSTGSTAMGRRGTKRSPYYSVCVWLCQSIIHILQLGQIWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFK8</accession>
    <name>A0A3B5LFK8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFK8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009632"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009746.1">
        <property type="protein sequence ID" value="ENSXCOP00000009632.1"/>
        <property type="gene ID" value="ENSXCOG00000007338.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155098"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="4">
        <location>
            <begin position="35"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="318" mass="36243" checksum="63E6CD2F479E9BF9" modified="2018-12-05" version="1">MSKKHNSGQFTNPETPGYVGFANLPNQVHRKSVKKGFEFTLMVVGESGLGKSTLINSLFLTDLYPERVIPGAAEKIERTVQIEASTVEIEERGVKLRLTVVDTPGYGDAINNFIANVSFYNRVHCCFYFISPLGHGLKPLDVQFMKAIHNKVNVVPVIAKADTLTLRERERLKRRILDEIDEHGIKIYHLPDAESDEDEDFKEQTRILKASIPFAVVGSNQQIEAKGKKVRGRLYPWGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSDRLKRGGRLSSHGYLRRMQEMIAKMQAQMQNQGEGDGQHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LG89</accession>
    <name>A0A3B5LG89_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">TGF-beta family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LG89"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006874"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006962.1">
        <property type="protein sequence ID" value="ENSXCOP00000006874.1"/>
        <property type="gene ID" value="ENSXCOG00000005314.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163919"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007369">
        <property type="term" value="P:gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13759">
        <property type="entry name" value="TGF_beta_NODAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000026">
        <property type="entry name" value="Nodal homolog 3-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF159">
        <property type="entry name" value="NODAL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0339">Growth factor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="TGF-beta family profile" evidence="11">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="110" mass="12943" checksum="3154C03231F32FF6" modified="2018-12-05" version="1">RNVEGKSDLCRTEDMWIDFEKFNWSEWIVYPKRFNAYRCEGTCPTPVDETFAPTNHAYMQSLLKLHLPDKVPSLSCVPTRLAPLSMLYYENGRMVMRHHENMVVEECGCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LH71</accession>
    <name>A0A3B5LH71_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Transcriptional repressor CTCF</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">11-zinc finger protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">CCCTC-binding factor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">CTCFL paralog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the CTCF zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LH71"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007219"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007311.1">
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    <sequence length="798" mass="88926" checksum="90DD61EBB05514B5" modified="2018-12-05" version="1">MDGSSVEAVGEVDAPPKDFPSIQAVHSQEALVVDLLQQAAEAGGHVEGMVAAAQSQQALLEAGVGVSVQRGEVEMMVMDSLDPTLMQMKTEVAAGQQDMSYLLSDSAHQAAVATVDQTQIITLQVVNMEEQAALGLGELQLVQVPVSAAAVDALQQGTFVDTTAMPKSGDPVICHTLPLPEGFQVVKVGANGEVETVEQEEGVEAHSEEDEDDADVGQQLLEEGEEAAQPPNHHQNWAKDPDYQPPSGGIKKAKKGKKSRLRYAEGDKDMDVSVYDFEEEQQEGLLSEVNAEKVVGNMKPPKPTKIKKKGVKKTFQCELCSYTCPRRSNLDRHMKSHTDERPHKCHLCGRAFRTVTLLRNHLNTHTGTRPHKCTDCDMAFVTSGELVRHRRYKHTHEKPFKCSMCDYASVEVSKLKRHIRSHTGERPFQCSLCSYASRDTYKLKRHMRTHSGEKPYECYICHARFTQSGTMKMHILQKHTENVAKFHCPHCDTVIARKSDLGVHLRKQHSFIETGKKCRYCDAVFHERYALIQHQKSHKNEKRFKCDLCDYCCRQERHMVMHRRTHTGEKPFACSQCEKTFRQKQLLDMHFKRYHDPNFVPTAFVCPKCSKTFTRRNTMARHSENCNGEVEEFENGAATPKKGRRGRKRKMRSRRDEDDSEDDIPEQDEDLEGMELDQAPAVIPVVAPDEPPVKRKRGRPPKNPKPQTPSRAGTASSVAAMIQVEDELTGAVENIIVKKEGGDAATSVKQGADLAVEGGVGGGEGVEEVELTVNQETTAATAANGDLTPEMILSMMDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHB9</accession>
    <name>A0A3B5LHB9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphodiesterase</fullName>
            <ecNumber evidence="10">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PDE4B</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.4.53"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:25278"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHB9"/>
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        <property type="protein sequence ID" value="ENSXCOP00000009985.1"/>
        <property type="gene ID" value="ENSXCOG00000007576.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155190"/>
    <dbReference type="UniPathway" id="UPA00762">
        <property type="reaction ID" value="UER00747"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006198">
        <property type="term" value="P:cAMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000001">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040844">
        <property type="entry name" value="PDE4_UCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18100">
        <property type="entry name" value="PDE4_UCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0114">cAMP</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="12">
        <location>
            <begin position="264"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="588"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="651"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="340"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="340"/>
            <end position="344"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="381"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="381"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="381"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="549"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="662" mass="74652" checksum="E55D4D052A5E7D4C" modified="2018-12-05" version="1">IRFVWDKHGGNVFILFVSSFSFEVENGPSLCCSPSDPQVSPGSGLVLHPNLAGHGQRRESFLYRSDSDYELSPKSLSRNSSIVGELCVVDIYGINQPAVLASLRTVRNNFTTLTNIQCASNNAAMDPLGPDYVSVCLLADESYQKLAMETMEELDWCLDQLETIQTYRSVSDMASNKFKRMLNRELNHLSEMSRSGNQVSEYISNTFLDKQNELELPCPLQKVRERKRKQSQQQQVGMMTQISGVKKVNHTSSTLGNVSNRFGVKTDQEELLSKDLEDVNKWGLNIFKVAEHSHNRPLTCVMYKIFQERDLMRTFKIPTDTFVTFMLTLEDHYHSDVTYHNSLHAADVAQSTHILLSTPALDAVFTDLEILAAIFGAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEDNCDIFQNLSKKQRQMLRRMVIDMVLATDMSKHMNLLANLKTMVETKKVTSSGVLLLDNYTDRMQVLCNMVHCADLSNPTKPLDLYRQWTDRIMDEFFHQGDREREKGMEISPMCDKHTASVERTQVSFIDFIVHPLWETWADLVHPDAQDILDTLEDNRNWYQSMIPQSPSPPFYTSDGEGGQHGEDCSPASKFPFDLTTDDGTPADAAVRSDCASRGGSSDLDGTEMTTCDVSSAET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHV8</accession>
    <name>A0A3B5LHV8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHV8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009377.1">
        <property type="protein sequence ID" value="ENSXCOP00000009266.1"/>
        <property type="gene ID" value="ENSXCOG00000007059.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177112"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784:SF3">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 4 MEMBER K-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017363517" description="C-type lectin domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="34"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="150" mass="17453" checksum="A0B04C9F7DBDDAA1" modified="2018-12-05" version="1">PAFFLLIKHFQLILVAFYTSNFASTGLCSLCLCVHQHQYVLFHSPKTWTDAQSYCRETCIDLATIEDMKEMDMALQSVGDDYSDAVWIGLHRGKELTWHWSLADKYFYKDGEKNYIKWRKPLSLNNCNESGKWGDRKCTEVHPVLCKCSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHZ2</accession>
    <name>A0A3B5LHZ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHZ2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011883.1">
        <property type="protein sequence ID" value="ENSXCOP00000011748.1"/>
        <property type="gene ID" value="ENSXCOG00000008880.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="35"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="49"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="101"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="149" mass="16466" checksum="E01DCDE1882E83D5" modified="2018-12-05" version="1">MTLNCSSRCFTAPPRKSKKDFVSFSNIFSPSQDPCFNCGQIGHWARECPQKKRDRSRGRGFHHHQGRPNPRGGFGSRGRDTLTAAPRRRPAPPPRARCSRSRCVSAGGAAVAAASRGRRPPSPPSRATRRRPAASWPRFGTREASSSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJL8</accession>
    <name>A0A3B5LJL8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000010845"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010970.1">
        <property type="protein sequence ID" value="ENSXCOP00000010845.1"/>
        <property type="gene ID" value="ENSXCOG00000008190.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161905"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098831">
        <property type="term" value="C:presynaptic active zone cytoplasmic component"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019992">
        <property type="term" value="F:diacylglycerol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017075">
        <property type="term" value="F:syntaxin-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061789">
        <property type="term" value="P:dense core granule priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099525">
        <property type="term" value="P:presynaptic dense core vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035249">
        <property type="term" value="P:synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016081">
        <property type="term" value="P:synaptic vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016082">
        <property type="term" value="P:synaptic vesicle priming"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20859">
        <property type="entry name" value="C1_Munc13-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08394">
        <property type="entry name" value="C2A_Munc13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04027">
        <property type="entry name" value="C2B_Munc13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08395">
        <property type="entry name" value="C2C_Munc13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1100:FF:000001">
        <property type="entry name" value="Protein unc-13 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000002">
        <property type="entry name" value="Protein unc-13 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000014">
        <property type="entry name" value="protein unc-13 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000031">
        <property type="entry name" value="Protein unc-13 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000001">
        <property type="entry name" value="Protein unc-13 homolog B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010439">
        <property type="entry name" value="MUN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014770">
        <property type="entry name" value="Munc13_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014772">
        <property type="entry name" value="Munc13_dom-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027080">
        <property type="entry name" value="Unc-13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037302">
        <property type="entry name" value="Unc-13_C2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10480">
        <property type="entry name" value="PROTEIN UNC-13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10480:SF15">
        <property type="entry name" value="PROTEIN UNC-13 HOMOLOG A-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06292">
        <property type="entry name" value="MUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01145">
        <property type="entry name" value="DUF1041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51258">
        <property type="entry name" value="MHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51259">
        <property type="entry name" value="MHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="9">
        <location>
            <begin position="431"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="537"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="MHD1" evidence="10">
        <location>
            <begin position="968"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="domain" description="MHD2" evidence="11">
        <location>
            <begin position="1194"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="1349"/>
            <end position="1474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="166"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="272"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="286"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1520" mass="173361" checksum="F335BEB780E50A74" modified="2018-12-05" version="1">MKTDGVPVCHHVPLCVPTVKKAKLDGPQEKFNTYVTLKVQNVKSTTIAVRGNLPCWEQDFMFEINRLDLGLTVEMWNKGLIWDTMVGTVWIPLRSIRQSNEEGPGEWLTLDSQVIMADSEICGTKDPTLHLVLLDTRFELPLDIPEDEARYWAKKLEQLNAMRDQDVSCQPVLDEDPDSAVDDRDSDYRSETSNSIPPPYYSTSQPNASVHQYPISHQHRSSRGLSYPRSGNNQDLDYHHQRTISPTGSYASSAELSRCSSQLSEEDYGGEYGERDPYNENDSYHSCHSSVSYSKGSPDWDPDETQGAYSDEGGYSKDGGEEVALYEGDDGELYEGEEEGLYEDEEMMYDDEDLYNLDGTLSEDLEPMGGALYSIDSMPDLRKRKAIPLVRDLVRNDKKANTQSTIYATLKSHVYKKTLQALIYPISSTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEVFGLDKTTHSTHMKQIKQSVLDGTSKWSAKISITVACAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEESFHFECHNSSDRIKVRVWDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRMHISVEIKGEETVAPYHVQYTCLHEHLFQYTTEDQNGGVVKIPEAKGDDAWKVYFDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTQSSNNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHDLYNREYQTDPAVPPDEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWNMFAQDMKYAMEEHEKNRLCKSADYMNLHFKVKWLYNEYCKELPTFQKHVPEYPAWFEPFVVMWLDENEEVSRDFLHGALERDKKDGFQVTSEHALFSCSVVDVFSQLNQSFEIIRKLECPDPQIVGNYMKRFAKFLMYHDFMRFPSLRLSPCIIINNIQQLRVQLEKMFEAMGGKDVSFLSTVVCSCFFFLWRHSFQPQIEDNVRQMGDILAQVKGQTVPANGSVVQEADNVLQPIMDFLDSNLSLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLPTLTDQTGTQMIFNAAKELGQLSKLKEHMGREEAKALTPKQCAVIELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLHYALSLYTQATDKLIRKFVLSQHAQASGVDAVGEVVMNVDILPQPNGEHKISIKVVAANDLKWKAQGFRPFVEVYIIGPHLSDKKRKFATKSKNNSWSPKFNESYQYSLGTEVGPESYELQVCVKDYCFAREDRTVGMAVLQVKDIANKASVTCWLPLGRRVHMDETGLTVLRILSQRNNDDVAKEFVKLKSDQRSAEEGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLA0</accession>
    <name>A0A3B5LLA0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="7">The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLA0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010336"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010459.1">
        <property type="protein sequence ID" value="ENSXCOP00000010336.1"/>
        <property type="gene ID" value="ENSXCOG00000007834.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162200"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03761">
        <property type="entry name" value="proteasome_beta_type_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF15">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6 9" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0888">Threonine protease</keyword>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="287" mass="31708" checksum="5F1FA381901A0405" modified="2018-12-05" version="1">MALEDICCFKDKPAQQLFPFPALETDYHNLELERLKTSQTNWGDPQHIFIPPFPQATPFISRRTSRFRPPNHALTVQRPFPLSHGTTTLGFIFQGGVIAAADTRASAGGLVACPAVHKITPIHSHLVVTSSGSGADCMLWERILIREIRLYELRHKRRLSIRASAKLLSFMLHPFKGTDVCVALTLCGWEKEGGIIYVCSDGARLQGDIISVGSGSPYAYGVLDGGLSWGLSKEEAISLAREAVFRATHRDAYSGNNVDLFHIDAQESSLSRLQPRQVHLFPSDEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMH2</accession>
    <name>A0A3B5LMH2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">PDS5 cohesin associated factor B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PDS5B</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMH2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013263"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013427.1">
        <property type="protein sequence ID" value="ENSXCOP00000013263.1"/>
        <property type="gene ID" value="ENSXCOG00000010053.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157257"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19953">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:001146">
        <property type="entry name" value="PDS5 cohesin associated factor B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000064">
        <property type="entry name" value="Sister chromatid cohesion protein PDS5 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039776">
        <property type="entry name" value="Pds5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663">
        <property type="entry name" value="ANDROGEN INDUCED INHIBITOR OF PROLIFERATION AS3 / PDS5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663:SF1">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN PDS5 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20168">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1135"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1137"/>
            <end position="1155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1176" mass="133978" checksum="DCED20F3A6D89D37" modified="2018-12-05" version="1">MAHSKARATDGKVTYPPGVKEISDKISKEEMVRRLKMVVKTFMDMDQDSEEEKELYLNLALHLASDFFLKHPDKDVRLLVACCLADIFRIYAPEAPYTSPDKLKDIFMFITRQLKGLEDTKSAQFNRYFYLLENIAWVKSYNICFELEDSNEIFTQLYRTLFQVINNGHNQKVHMHMVDLMSSIICEGDTVSQELLDTVLVNLVPAHKNLNKQAYDLAKALLKRTAQAIEPYITNFFNQVLMLGKTSVSDLSEHVFDLILELYNIDSHLLLSVLPQLEFKLKSNDNDERLQVVKLLAKMFGAKDSELAAQNKPLWQCYLGRFNDIHVPIRLECVKFASHCLMNHPDLAKDLTEYLRVRSHDPEEAIRHDVIVSIVTAAKKDLSLVNDALLNIVKERTLDKRWRVRKEAMMGLASIYRKYSLQGEGGREASKQISWIKDKLLHIYYQNSIDDRLLVERVFAQYMVPFNLDTTERMKCLYYLYATLDTNAVKALNEMWKCQNMLRNHVKDLLDLIKKPKSEASSKAVFAKVMVITRNLPDPGKAQDFVKKLAQVLEDDERIRDQLETLVSPTCSCKQAEVCVRDITKKLGSPKQPSNPFLEMVKFLLERIAPVHIDTESISALIKQVNKSIDGTADDEEEGVPTEEAIRAGLELLKVLSFTHPVSFHSAETFESLLSCLKMDDEKVAEAALQIFKNTGNKMEESFPHIKSVLLQVLQTKAKRGPPRQAKYAIHCINAMFTNRDTHFAQIFEPLHKSLDPANLEQLITPLTTLGHLAQLAPEQFAAPLKSLVANFIVKDLLMNDRIPGKKTTKLWVSDDEVSPETMAKIQGIKLMVRWLLGVKNNQSKSGNSTLRMLTAILHSDGDLTEQGRMGKPDMSRLRLAAACALLKLAQEPCYHEIITLEQYQLCALVINDECYQVRQCFAQKLHRGLCRLRLPLEYMAVFALCAKDPVKERRAHARQCLVKNVNIRREYLKQHAAISDKLLSLLPEYVVPYTIHLLAHDPDYVKVQDIEQLKEIKEALWFVLEIIMAKNENNSHAFIRKMVENIKQTKDAQASSDPKTNEKLYTVCDVAMNIIMSKSTTYSLESPKDPVLPSRFFTKPDKNFSNTKNYLPPEMKAFFTPGKPKSANVLGAVNKPLSSAGKQLQSKASRMETTSNNDSSSNPGSPQRGKTRYGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LN21</accession>
    <name>A0A3B5LN21_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calpain catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LN21"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009259"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009370.1">
        <property type="protein sequence ID" value="ENSXCOP00000009259.1"/>
        <property type="gene ID" value="ENSXCOG00000007054.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156128"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00214">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.380:FF:000003">
        <property type="entry name" value="Calpain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000001">
        <property type="entry name" value="Calpain-1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033883">
        <property type="entry name" value="C2_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022682">
        <property type="entry name" value="Calpain_domain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022683">
        <property type="entry name" value="Calpain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF381">
        <property type="entry name" value="CALPAIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01067">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00720">
        <property type="entry name" value="calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="7">
        <location>
            <begin position="25"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="603" mass="69565" checksum="79126B089AE6F466" modified="2018-12-05" version="1">ISTMPVAYRNQHYAELKKQHNKENLFEDPEFPATNSSIYYKDPPEGVEVVEWKRPGEICENPHLFVEGISSHDLNQGSEGNCWMVAACSCLALKSDLWKKVIPDWKEQEWDSNYAGIFHFRFWIFGEWIDVVIDDRLPTSNGQLIYCQSKQNNEFWSALLEKAYAKLFGCYESLDGGNTRDAVVDFSGAVAEAIDLQEEKYYTDKKTQDKLFDNLLKSLTMSKLNIFFEISQPQGFELESRTDNGLVRGHAYSVTDVKRVQLSHGLRTYFKNDTIPLIRLRNPWGKIEWTGAWSDSSEEWSKIGDTERGNLGITVADDGEFWMSFTDWCKNFTDADICHLMNTSLISTEKNWNEAVNFGKWTRHSNPLLNRCGGCINNKETFLQNPQYLFDVTKESDEVLISLQQKDRRIYRKEGQGDNLDIGFRVVKVEVNRKYRLHNMETLKSMHKSTFLPARMMFMRCELPKGRYVVIPTTFDAFIEGEFMLRLYTDVDSGCRYETDGKTNYFKMLMWTLNDGFLGKSFYSNTTNHLLCYLLLNLSGLFQLWSDDLGSDKFLGQVTLSGRPDDVSHPQQLQLKNKGSKEADNMPGKIALRILTSDELTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNR0</accession>
    <name>A0A3B5LNR0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSXCOT00000012811.1">
        <property type="protein sequence ID" value="ENSXCOP00000012657.1"/>
        <property type="gene ID" value="ENSXCOG00000009565.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286984"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="3">
        <location>
            <begin position="23"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="96"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="126" mass="14032" checksum="EBF72419919F33F3" modified="2018-12-05" version="1">MPEPAKSAPKKGSKKAVTKTAGKGGKKKRRTRKESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDIFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXMPHYKSSQMSHDKSSQMSHIKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQ32</accession>
    <name>A0A3B5LQ32_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetraspanin-9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQ32"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010007.1">
        <property type="protein sequence ID" value="ENSXCOP00000009889.1"/>
        <property type="gene ID" value="ENSXCOG00000007505.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158225"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03165">
        <property type="entry name" value="NET-5_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000008">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF41">
        <property type="entry name" value="TETRASPANIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="109"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="243"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="279" mass="31572" checksum="7591759959A9F4E8" modified="2018-12-05" version="1">MEKSSSEESAIHRSPSVDSRDSDFPQASTSGRPFGGRGFTAGAFYGSTGPRKNTQQGPAGAAADTGAADKTNKYSSPKGSKYVVFYLDLSFVFLLEFKKCNMARGCLCCLKYLMFIFNLIFWFFIVLLIILLAELILIILFFVYSDKLQVSENAKQDLKDGLALYNSENNIGLRNAWNIIQAEWKCCGVIAYTDWHEALKEKVVPDRCCQEHYQNCGRNSTNMFWNRGCFEKVEEWLDENKHLLGTIGMVILVVQQLLGMAFSMTLFHHIHRTGKKYDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQK4</accession>
    <name>A0A3B5LQK4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQK4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014393"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014577.1">
        <property type="protein sequence ID" value="ENSXCOP00000014393.1"/>
        <property type="gene ID" value="ENSXCOG00000010916.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166856"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000113">
        <property type="entry name" value="homeobox protein Hox-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046327">
        <property type="entry name" value="HXA1/B1/D1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45946:SF5">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45946">
        <property type="entry name" value="HOMEOBOX PROTEIN ROUGH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="227"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="229"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="21"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="280"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="307"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="317"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="331" mass="36352" checksum="0743025C7E171D4D" modified="2018-12-05" version="1">MDNMNSFVEYSICNRPGNGSYPHHGLSAHETRGVSQHHQQHPVNHLEHQQQPHTHNGFQHHQNQSSQSGLLTQYTNGSGCTSGAYAGQTCVTNSEYVAITGPPSSVQPQYFMEESVASTYYHQSAFPTSTPAVGPSYSGLSGSYCGPQGPLASSQYPQQIGGGLETVAYLGFPQAGGYGELAGSQDRGREDEDSQQSGQGQTFDWMKVKRNPPKTGLKVSDFGLTGVHNNAMRTNFSTRQLTELEKEFHFSKYLTRARRVEIAATLELNETQVKIWFQNRRMKQKKREREGAGTTARSYSSETRSGFVKDLEDTDHSSLSTSPGASPSSET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQQ2</accession>
    <name>A0A3B5LQQ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Family with sequence similarity 92 member A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Cilium</location>
            <location evidence="3">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Mitochondrion inner membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Matrix side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the CIBAR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQQ2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013115"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013276.1">
        <property type="protein sequence ID" value="ENSXCOP00000013115.1"/>
        <property type="gene ID" value="ENSXCOG00000009937.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010285"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035869">
        <property type="term" value="C:ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07598">
        <property type="entry name" value="BAR_FAM92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000047">
        <property type="entry name" value="protein FAM92A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035590">
        <property type="entry name" value="BAR_CBAR1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009602">
        <property type="entry name" value="CBAR/FAM92"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21223:SF4">
        <property type="entry name" value="CBY1-INTERACTING BAR DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21223">
        <property type="entry name" value="CBY1-INTERACTING BAR DOMAIN-CONTAINING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06730">
        <property type="entry name" value="FAM92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="13" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="12 20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0282">Flagellum</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="114"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00029449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="23">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="33840" checksum="FCDEE55F96A65A07" modified="2018-12-05" version="1">MSRTPDARVRDNQTRKIQENITKVEKHFGDMCQLFAAYARKTARLRDKADLLVREIGLYADTETPDLKRGMKQFAEHLAKIQDYRQAEVERLEARVIEPLKSYGAVVKRKREDLKATQSARDREAKQMAQLEKTRLRNPSDRQIIAETELQRATMDATRTTKQLEETIDEFERQKIQDIKKVFGEFVTVEMSFHAKALEIYTVAFQSIQSVDEDADLEVFRNCLHPPDFQSRLDIVRANSKTSLNHTGSFLSTSGTLQLLVMHSTNVSFMEECQEESDYYKKIKSGSSCTFPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQW1</accession>
    <name>A0A3B5LQW1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arf-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQW1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011791"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011927.1">
        <property type="protein sequence ID" value="ENSXCOP00000011791.1"/>
        <property type="gene ID" value="ENSXCOG00000008913.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158387"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051718">
        <property type="entry name" value="ARF_GTPase-activating"/>
    </dbReference>
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        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705:SF1">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="2">
        <location>
            <begin position="24"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="94" mass="10880" checksum="7CD25119E91E4C87" modified="2018-12-05" version="1">MNTKRTGDRSRRRKWTITQGILGPRWASWNIGVFLCIRCADIHRKLGVHISRVKSVNLDTWTNMKEIGNSRTRAIFEARIPDGFQRPSSDQAIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LR13</accession>
    <name>A0A3B5LR13_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LR13"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010224"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010347.1">
        <property type="protein sequence ID" value="ENSXCOP00000010224.1"/>
        <property type="gene ID" value="ENSXCOG00000007758.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155927"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000026">
        <property type="entry name" value="APOBEC1 complementation factor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21245">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="57"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="128" mass="14470" checksum="6F9F3A65A9FCD4B1" modified="2018-12-05" version="1">PEHSHKSTSFGLVSCAKEMALLALMEKTGYNMVQENGQRKYGGPPPGWEGPAPPRGCEVFVGKIPRDMFEDELVPLFEKAGKIYEFRLMMEFSGENRGYAFVMYTNRWRSVTASKVGNTHRFSLLNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LR86</accession>
    <name>A0A3B5LR86_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LR86"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013562"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013729.1">
        <property type="protein sequence ID" value="ENSXCOP00000013562.1"/>
        <property type="gene ID" value="ENSXCOG00000010270.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159922"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061578">
        <property type="term" value="F:K63-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF58">
        <property type="entry name" value="UBIQUITINYL HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="OTU" evidence="8">
        <location>
            <begin position="21"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="7">
        <location>
            <begin position="265"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="325"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="466"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="342"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="478"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="785" mass="86802" checksum="EF2ED19419FC671E" modified="2018-12-05" version="1">MQKGLKKYFVNMDEYLSSLGLYRKMVARDASSLFRAVSEQLYFSQNYHQKIRLDCASFMRANRCNFEPFVEGSFEKYLERLEDPKETVGQVEIKALSQLYRRCFLIYRYPGKPATVISENDFEDKVTLCCSINGHYDIIYGRSYPASAALCQSLLYELLYTQVFGVEGVELWQAMETFRAGGRRYRNSLSVCSDVDVGYDAPEDRLHRDEAEPPAEAPPTSRLCLPYKVLKSLDGDVYRNLEFDVWQDTCKEMQKTDYMVFAGRQYFLGDKCQVRLEPKGKFYNAFIQEVGTHSTAVTVFIEELGEKHLVPLTDLKPVNPVPAWNVAAPPRKGDPDPESRGQRHHRHRYFRKSRGSSGAKGAELLLPPPVSYGGPALSPLPPRFQPAGHPRPPLPPPARFLNRQLIGPQLTYYHPGRRYYHDYENYAFRSRRSRRHLLAVSKECQFGFPAEAVEEAADLDPPMTFYQLEDAGDAGPAVAPPIGAPSPPSGSSYRVQRAAASRPPASPPEATPPCSSEDDQADASTVEDQGEWLTFQNKSLGERSSTRRSTCSAPRVRIGTLLPCFLLGQEVTSCCVVDQSYQMPGVYPTDEPAATQVTTLTPDLIGFLSSVSMMISAAAPWLVSESGEPLCTVLAPPSYSYDPSGSDLPRDCRVVQYYYNLGVQVGPAPSAEGPTSIMTPASPGSGPSGLLYPDQTALPAPTLLHLPFEAPPPAGYLPAAPHPPLPHLPQSSYHPCVPPSAHWGALSTSGHAPRVYCPAPSPHVVGYITAPHPHHTPTHYVPPSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTA9</accession>
    <name>A0A3B5LTA9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipase A2-like central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTA9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012611"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012763.1">
        <property type="protein sequence ID" value="ENSXCOP00000012611.1"/>
        <property type="gene ID" value="ENSXCOG00000009535.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165341"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.90.10">
        <property type="entry name" value="Phospholipase A2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016090">
        <property type="entry name" value="PLA2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036444">
        <property type="entry name" value="PLipase_A2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12253">
        <property type="entry name" value="RH14732P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05826">
        <property type="entry name" value="Phospholip_A2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48619">
        <property type="entry name" value="Phospholipase A2, PLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phospholipase A2-like central" evidence="2">
        <location>
            <begin position="3"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="155" mass="17972" checksum="C91A01E76D91C3C7" modified="2018-12-05" version="1">MYTCCRTHDHCPHVIHAFSSNYGYTNFKWHSICHCDCDEELKACLRQVNDTSSRVVGQAFFNVIGVPCFDFAYEDGRNPSRAKRRSRTSSPPPRTSSRCSPPSPRLKAPTQTPTRRRRKPRRRRGGRTLGRRRKQTGNIKRKGRAGRGSRKRRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTB3</accession>
    <name>A0A3B5LTB3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTB3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011049"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011176.1">
        <property type="protein sequence ID" value="ENSXCOP00000011049.1"/>
        <property type="gene ID" value="ENSXCOG00000008346.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164803"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF101">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="202" mass="22831" checksum="C630A5789F439FD4" modified="2018-12-05" version="1">MAMELCAGGDLMDRICDRRRLGEREVRRYTRQILSAVEHLHKHGIVHRDLKIENFLLDEHNNIKIVDFGLSNTLKPDSLSLELLNTQCGSPAYAAPELLAHKKYGSKVDVWSMYVTNSPFVCPDHIKTSSLFLPRVRNANVMSCFCLVLLSHRGVSMFAMLTGTLPFTVEPFNIKQLHHKMVNKDISDIPGDISKGQNITTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTR4</accession>
    <name>A0A3B5LTR4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Doublesex- and mab-3-related transcription factor A2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Doublesex- and mab-3-related transcription factor 5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May be involved in sexual development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DMRT family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027882599.1">
        <property type="nucleotide sequence ID" value="XM_028026798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTR4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014240"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014417.1">
        <property type="protein sequence ID" value="ENSXCOP00000014240.1"/>
        <property type="gene ID" value="ENSXCOG00000010786.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114150416"/>
    <dbReference type="KEGG" id="xco:114150416"/>
    <dbReference type="CTD" id="63950"/>
    <dbReference type="GeneTree" id="ENSGT00940000161649"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060128">
        <property type="term" value="P:corticotropin hormone secreting cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007281">
        <property type="term" value="P:germ cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007548">
        <property type="term" value="P:sex differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021537">
        <property type="term" value="P:telencephalon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1040.10:FF:000001">
        <property type="entry name" value="doublesex- and mab-3-related transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1040.10">
        <property type="entry name" value="DM DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001275">
        <property type="entry name" value="DM_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036407">
        <property type="entry name" value="DM_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005173">
        <property type="entry name" value="DMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026607">
        <property type="entry name" value="DMRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046472">
        <property type="entry name" value="DMRT5_1_DMB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322">
        <property type="entry name" value="DOUBLESEX AND MAB-3 RELATED TRANSCRIPTION FACTOR DMRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322:SF76">
        <property type="entry name" value="DOUBLESEX- AND MAB-3-RELATED TRANSCRIPTION FACTOR A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00751">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03474">
        <property type="entry name" value="DMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20624">
        <property type="entry name" value="DMRT5_DMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00301">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82927">
        <property type="entry name" value="Cysteine-rich DNA binding domain, (DM domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS40000">
        <property type="entry name" value="DM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50809">
        <property type="entry name" value="DM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DM" evidence="11">
        <location>
            <begin position="57"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="DM" evidence="9">
        <location>
            <begin position="57"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="163"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="230"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="449" mass="47712" checksum="F8B8DF74089B8102" modified="2018-12-05" version="1">MDLRPELPTVSSASQVHPGAAAAAAAASIPVSMASNLLRGPPLLLRAADKYPRTPKCARCRNHGVVSALKGHKRYCRWKDCMCAKCTLIAERQRVMAAQVALRRQQAQEENEARELQLLYGTAEGLALAAANGIIPPRPNYEVFGSVNSESNSESSIQKYELFPKTQLSGSTTTQKSVGKPASTESDSAPGMSSPDGRHGGGSGSENGDSESFINSPVSKPLKDGEETPGSVSSLGSDSGSETDKEEQEPSPSSAASRHMNAIDILTRVFPSHKRSVLELVLQGCGKDVVQAIEQILNNSGAQAPNKAGPEETWTAERMLQSAQQPPAGSATAATTTRPMLPGAMTLSNRSAFSPLQPNAPHFGADPGTYPLGTHLGLNPLRLAYSAHSRGLAFMTPYSTTGLMPTLGFRPPMDYAFSDLIRDRTMLHKEQGYASGLYGPLVNNTPDKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTW2</accession>
    <name>A0A3B5LTW2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="35">Chloride intracellular channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="37 38">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="37" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="37" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="34">In the soluble state, catalyzes glutaredoxin-like thiol disulfide exchange reactions with reduced glutathione as electron donor. Can insert into membranes and form voltage-dependent multi-ion conductive channels. Membrane insertion seems to be redox-regulated and may occur only under oxidizing conditions. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis). Promotes cell-surface expression of HRH3.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>bromide(in) = bromide(out)</text>
            <dbReference type="Rhea" id="RHEA:75383"/>
            <dbReference type="ChEBI" id="CHEBI:15858"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>choline(out) = choline(in)</text>
            <dbReference type="Rhea" id="RHEA:32751"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>fluoride(in) = fluoride(out)</text>
            <dbReference type="Rhea" id="RHEA:76159"/>
            <dbReference type="ChEBI" id="CHEBI:17051"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>iodide(out) = iodide(in)</text>
            <dbReference type="Rhea" id="RHEA:66324"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>nitrate(in) = nitrate(out)</text>
            <dbReference type="Rhea" id="RHEA:34923"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>thiocyanate(in) = thiocyanate(out)</text>
            <dbReference type="Rhea" id="RHEA:75347"/>
            <dbReference type="ChEBI" id="CHEBI:18022"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Microtubule organizing center</location>
            <location evidence="5">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="32">Cytoplasmic vesicle membrane</location>
            <topology evidence="32">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="35">Membrane</location>
            <topology evidence="35">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="35">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="35">Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 35">Belongs to the chloride channel CLIC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTW2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015443"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015635.1">
        <property type="protein sequence ID" value="ENSXCOP00000015443.1"/>
        <property type="gene ID" value="ENSXCOG00000011311.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155017"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03061">
        <property type="entry name" value="GST_N_CLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000236">
        <property type="entry name" value="Chloride intracellular channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000001">
        <property type="entry name" value="Chloride intracellular channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002946">
        <property type="entry name" value="CLIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053823">
        <property type="entry name" value="CLIC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00862">
        <property type="entry name" value="O-ClC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45476:SF5">
        <property type="entry name" value="CHLORIDE INTRACELLULAR CHANNEL 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45476">
        <property type="entry name" value="CHLORIDE INTRACELLULAR CHANNEL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22441">
        <property type="entry name" value="CLIC-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13410">
        <property type="entry name" value="GST_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01263">
        <property type="entry name" value="INTCLCHANNEL"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="22 35" id="KW-0868">Chloride</keyword>
    <keyword evidence="20 35" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9 35" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="25" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="21" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="24 35" id="KW-0407">Ion channel</keyword>
    <keyword evidence="17 35" id="KW-0406">Ion transport</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="23" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="38" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 35" id="KW-0813">Transport</keyword>
    <keyword evidence="12 35" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="domain" description="GST C-terminal" evidence="36">
        <location>
            <begin position="95"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00024145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00035073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00035085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00036811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00036895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00037855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00044702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00045955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="RuleBase" id="RU362009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="36">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="267" mass="30172" checksum="8C03335DCF53FAAC" modified="2018-12-05" version="1">MLLISPGRELLPATALRTRKKTVPFLSQAYCALFLDHSAGSDGESIGNCPFSQRLFMILWLKGVVFNVTTVDLKRKPADLQNLAPGTHPPFITFNGEVKTDVNKIEEFLEDVLSPPKYIKLGAKHPESNTAGMDIFAKFSAYIKNSKPDANEALERGLLKTLQKLDDYLRSPLPDEIDHNSIEDIKFSSRKFLDGEEMTLADCNLLPKLHIVKVVTKKYRGFDIPKEMTSIWKYLNNAYTREEFTNTCPSDKEIEIAYGDVAKRLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LU72</accession>
    <name>A0A3B5LU72_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LU72"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014687"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014873.1">
        <property type="protein sequence ID" value="ENSXCOP00000014687.1"/>
        <property type="gene ID" value="ENSXCOG00000011150.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158752"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01241">
        <property type="entry name" value="PH_PKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000033">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000027">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000838">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039026">
        <property type="entry name" value="PH_PKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PH" evidence="12">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="112"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="371"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="412"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="442" mass="51205" checksum="BD911DA5E3D63BAF" modified="2018-12-05" version="1">DGTFIGYKERPQDVDQLETPLNNFSVAQCQLMKTERPKPNTFIIRCLQWTTVIERTFHVETPEEREEWTKAIQAVAEGLHKQEEERMDSSPEPMDMEVYLTKPRQKVTMHDFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSKHPFLTGLKYSFQTHDRLCFVMEYANGGELFFHLSRDRVFSEERARFYGAEIVSALDYLHAERNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHERLFELILMEDIRFPRTLGPEARSLLSGLLKKDPMQRLGGGPDDAKEIMQHKFFAGINWQDVYEKKLVPPFKPQVTSETDTRYFDEEFTGQIITITPPGQDDSMESFDSEQRPHFPQFSYSASGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUV3</accession>
    <name>A0A3B5LUV3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Haloacid dehalogenase-like hydrolase domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027857739.1">
        <property type="nucleotide sequence ID" value="XM_028001938.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027857740.1">
        <property type="nucleotide sequence ID" value="XM_028001939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUV3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011609"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011741.1">
        <property type="protein sequence ID" value="ENSXCOP00000011609.1"/>
        <property type="gene ID" value="ENSXCOG00000008762.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114135030"/>
    <dbReference type="KEGG" id="xco:114135030"/>
    <dbReference type="CTD" id="81932"/>
    <dbReference type="GeneTree" id="ENSGT00390000015582"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16415">
        <property type="entry name" value="HAD_dREG-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.720">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051828">
        <property type="entry name" value="HAD-like_hydrolase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011949">
        <property type="entry name" value="HAD-SF_hydro_IA_REG-2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044924">
        <property type="entry name" value="HAD-SF_hydro_IA_REG-2-like_cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02252">
        <property type="entry name" value="DREG-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46191">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46191:SF2">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="243" mass="27263" checksum="F2CCB03E5590E3C0" modified="2018-12-05" version="1">MPRVPLRWVLWDIKDTLLKVRGSVGDHYSKEAGRWGLSLQPAEINAAFRETFRRYSSRYPNYGVSQGQTGQTWWIGVMRDTLAECKVNDPALLNTMATNLYQDFCSAEKWEVFPDSKTALEQCSSLGLKLGVVSNFDNRLETILRSCGLLSHFSFVVTSEAAGVAKPSPAIFHQALQKCGTSADRVAHVGDHYVYDYLASRSVGIHGFLLDRDDSHKAGDVPQEHRVVTLDELPSRLQELMIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LV75</accession>
    <name>A0A3B5LV75_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Angiopoietin-like 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027882774.1">
        <property type="nucleotide sequence ID" value="XM_028026973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LV75"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011739"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011873.1">
        <property type="protein sequence ID" value="ENSXCOP00000011739.1"/>
        <property type="gene ID" value="ENSXCOG00000008869.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114150532"/>
    <dbReference type="KEGG" id="xco:114150532"/>
    <dbReference type="CTD" id="544656"/>
    <dbReference type="GeneTree" id="ENSGT00940000155091"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005577">
        <property type="term" value="C:fibrinogen complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072377">
        <property type="term" value="P:blood coagulation, common pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060216">
        <property type="term" value="P:definitive hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042730">
        <property type="term" value="P:fibrinolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002244">
        <property type="term" value="P:hematopoietic progenitor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070527">
        <property type="term" value="P:platelet aggregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034116">
        <property type="term" value="P:positive regulation of heterotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045747">
        <property type="term" value="P:positive regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000001">
        <property type="entry name" value="Tenascin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037579">
        <property type="entry name" value="FIB_ANG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036056">
        <property type="entry name" value="Fibrinogen-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014716">
        <property type="entry name" value="Fibrinogen_a/b/g_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002181">
        <property type="entry name" value="Fibrinogen_a/b/g_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221">
        <property type="entry name" value="FIBRINOGEN ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47221:SF5">
        <property type="entry name" value="FIBRINOGEN C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00147">
        <property type="entry name" value="Fibrinogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00186">
        <property type="entry name" value="FBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56496">
        <property type="entry name" value="Fibrinogen C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51406">
        <property type="entry name" value="FIBRINOGEN_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017293942" evidence="7">
        <location>
            <begin position="23"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="Fibrinogen C-terminal" evidence="8">
        <location>
            <begin position="263"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="483" mass="55429" checksum="116AA660BF68A2F3" modified="2018-12-05" version="1">MQGPIWSLCALLCLSLWEESFCRSSRDTRRTKEFSLQRSKRAAQDADDKKCSYTFLVPEQRITGPICASTTGPEPDKDRVTRMDIADVREVLNKQRREIETLQLVVEVDGNLVNEMKLLRKESRNMNSRVTQLYMQLLHEIIRKRDNSLELAQLENRVLNVTSEMMRLASRYKELEARFATMAGVVNNQSILIAALEEQCMRTLGRSELPPVPPLVQVVPENIPLDNRFTNEIVRNNRAFLRDSRMEAPTARPFDPPPPQGTITSDGPFKDCYQVRRAGHSTNGMYLLKTDNNESLIQVWCEHSLDNGGWTVLQKRKDGSVNFFRNWENYKKGFGNIDGEHWLGLDNIYSLAKQGDYKLMVELEDWTGKKVYAEYSSFYLEPESEGYKLRLGTYQGNAGDSFSSNNGKQFTTLDRDRDEFSGNCAHFHKGGWWYNACGQTNLNGVWYSGGVYRSKFQDGIFWAAYGGGFYSLKSVRLLIRPID</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LW31</accession>
    <name>A0A3B5LW31_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">1-phosphatidylinositol-5-phosphate 4-kinase</fullName>
            <ecNumber evidence="11">2.7.1.149</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + ATP = 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83423"/>
            <dbReference type="ChEBI" id="CHEBI:84968"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:55993"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + GTP = 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:83423"/>
            <dbReference type="ChEBI" id="CHEBI:84968"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:55965"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12280"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57795"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.149"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:12281"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.1.149" evidence="11"/>
    <dbReference type="RefSeq" id="XP_027873151.1">
        <property type="nucleotide sequence ID" value="XM_028017350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LW31"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015080"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015269.1">
        <property type="protein sequence ID" value="ENSXCOP00000015080.1"/>
        <property type="gene ID" value="ENSXCOG00000011430.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114144473"/>
    <dbReference type="GeneTree" id="ENSGT00940000159874"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016309">
        <property type="term" value="F:1-phosphatidylinositol-5-phosphate 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17310">
        <property type="entry name" value="PIPKc_PIP5K2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 5-phosphate 4-kinase type-2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.810.10:FF:000003">
        <property type="entry name" value="Phosphatidylinositol 5-phosphate 4-kinase type-2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.810.10:FF:000004">
        <property type="entry name" value="Phosphatidylinositol 5-phosphate 4-kinase type-2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF22">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 5-PHOSPHATE 4-KINASE TYPE-2 BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="13">
        <location>
            <begin position="33"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="412" mass="46808" checksum="4B49569E56B176FA" modified="2018-12-05" version="1">MSSNCTSAAAVSASKTKTKKKHFIGQKVKLFRASEPILSVLMWGVNHTINELSNVPVPVMLMPDDFKAYSKIKVDNHLFNKENLPSRFKFKEYCPMVFRNLRERFAIDDQDYQNSLTRSAPLNSDSQGRFGNRFLTSYDHRFVIKTVSSEDIAEMHNILKKYHQFTVECHGNTLLPQFLGMYRLTVDGVETYMVVTRNVFSHRLTVHRKYDLKGSTVAREASDKEKAKELPTFKDNDFLNEGHKLKIGDDNKKYFLEKLKRDVEFLATLKIMDYSLLVGIHDVERAEQEEMDVEGAGDEEEFENDEMGRGVLTGSFGTPPDSPGNPLNCGGFFGPGEFDPSVDVYAIKSHDSAVKKEVYFMAIIDILTHYDAKKKAAHAAKTVKHGAGAEISTVNPEQYSKRFYEFMSNILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LW88</accession>
    <name>A0A3B5LW88_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">RPS6KA6</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LW88"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015397"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015589.1">
        <property type="protein sequence ID" value="ENSXCOP00000015397.1"/>
        <property type="gene ID" value="ENSXCOG00000011657.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159242"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05582">
        <property type="entry name" value="STKc_RSK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000010">
        <property type="entry name" value="Ribosomal protein S6 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000041">
        <property type="entry name" value="Ribosomal protein S6 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000013">
        <property type="entry name" value="Ribosomal protein S6 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000121">
        <property type="entry name" value="Ribosomal protein S6 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016239">
        <property type="entry name" value="Ribosomal_S6_kinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041906">
        <property type="entry name" value="RSK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000606">
        <property type="entry name" value="Ribsml_S6_kin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="31"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="17">
        <location>
            <begin position="282"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="375"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="492"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="37"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 15">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="381"/>
            <end position="389"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 15">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000606-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000606-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="694" mass="77974" checksum="991E53238A2B9E87" modified="2018-12-05" version="1">MSFAQEDGTYKEIPITHHVKEGCEKADPSQFELLKVLGQGSFGKVFLVRKIVGPDGGQLYAMKVLKKASLKVRDRVRTKMERDILVEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDVFTRLSKEVMFTEEDVKFYLAELALALDHLHNLGIVYRDLKPENILLDEAGHIKLTDFGLSKESVDADKKAYSFCGTVEYMAPEVVNRRGHTQSADWWSLGVLMFEMLTGTLPFQGKDRNETMNMILKAKLGMPQFLSLEAQSLLRMLFKRNPANRLGAGPDGVEEIKRHKLFRRELQPPFKPAAGKPDDTFCFDPEFTAKTPKDSPGIPPSANAHQLFKGFSFVAPAPMDENKSSPLLSILPIVQVHGGSTKFSDLYELQEDIGVGSYSICKRCVHRVSGADYAVKIIDKSKRDPSEEIEILMRYGQHPNIITLKDVYDEDRYVYLVTELMKGGELLDRILRQKFFSEREASAVLYTITKTVDYLHCQGVVHRDLKPSNILYMDDSGNPDSIRICDFGFAKQLRGGNGLLLTPCYTANFVAPEVLMRQGYDAACDIWSLGVLLYTMLAGYTPFANGPNDTPEEILLRIGSGKFSLTGGNWDTVSDSSKDLLSHMLHVDPHQRYTAEQVLKHSWITCRDSLPHFQLTRHDAPHLVKGAMAATYSALSQKTSQPVLEPVAASSLAQRRSMKKLTSTDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWL2</accession>
    <name>A0A3B5LWL2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Suppressor of forked domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWL2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015522"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015715.1">
        <property type="protein sequence ID" value="ENSXCOP00000015522.1"/>
        <property type="gene ID" value="ENSXCOG00000011737.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:mRNA binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045243">
        <property type="entry name" value="Rna14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008847">
        <property type="entry name" value="Suf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19980:SF0">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19980">
        <property type="entry name" value="RNA CLEAVAGE STIMULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05843">
        <property type="entry name" value="Suf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Suppressor of forked" evidence="4">
        <location>
            <begin position="18"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="107" mass="12416" checksum="19D09AC9E99479B3" modified="2018-12-05" version="1">MSTEGTAEQTAAEYIPEKVKKAEKKLEENPYDLDAWSILIREAQNQPIDKARKTHERLVSQFPSSGRFWKLFIEAEVNTFLFFSLVCMAETYSNIINYVIFTQSKCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWM6</accession>
    <name>A0A3B5LWM6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinetochore protein Nuf2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NUF2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWM6"/>
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        <property type="gene ID" value="ENSXCOG00000009261.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.60">
        <property type="entry name" value="Ncd80 complex, Nuf2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinetochore_Nuf2_N"/>
    </dbReference>
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        <property type="entry name" value="Nuf2_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03800">
        <property type="entry name" value="Nuf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Nuf2 N-terminal" evidence="10">
        <location>
            <begin position="4"/>
            <end position="114"/>
        </location>
    </feature>
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        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022618"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="280" mass="32143" checksum="08991BF162D75378" modified="2018-12-05" version="1">MTENTFPLHNADAIVNFYRSEVLIGQEAKQFTKGDLTPVPKPEAVQTLYMRILHLLYRFRPECHSMVPFSENIQYPGYHEGATAIISVYTRMRQFLPMCLVYDFSLNDLLAPSKYVFLQAARADMDKLQTYTRGNREAEKKIEQLTTIPPEQQAEADELAAALSELQATTVHEYQEVNVKNENIAEWKTKIAEKTQKLSQVKVDVSNLKEDITKLKSQIVESPEELKSQKEKMKEAAKKIKNSIVSRKMFGNPRLKKKTNKSEIDKQQIISLCFCVTDGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXL4</accession>
    <name>A0A3B5LXL4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXL4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014081"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014256.1">
        <property type="protein sequence ID" value="ENSXCOP00000014081.1"/>
        <property type="gene ID" value="ENSXCOG00000010664.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000285758"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="244" mass="25778" checksum="5A45DBED1788CFD8" modified="2018-12-05" version="1">LLLARNRLSQVSYPRASSASAYFSKRFFNKFCTADFSTSSNLPRLFPFCLQSPPRFLLCLQHPPRFLDCLQGFLASPVGLLTDLQGFIAIHVGLLADLQGVLASPVGLLDCFQGYLASPVGLLADLQGFLASTVGLLADFQGYLASPVGLLADLQGFLASPVGLLADIQGFIASPVASPFGLLADLQGFLASPVSLLDCLQGFIASPVGLLADLQGFLASPALENESDMVSVFSEGLGINEYII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZJ7</accession>
    <name>A0A3B5LZJ7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">DNA topoisomerase II binding protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
            <location evidence="4">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the TOPBP1 family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000016390"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016597.1">
        <property type="protein sequence ID" value="ENSXCOP00000016390.1"/>
        <property type="gene ID" value="ENSXCOG00000012377.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157001"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060216">
        <property type="term" value="P:definitive hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902165">
        <property type="term" value="P:regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="BRCT_TopBP1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="BRCT_TopBP1_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17749">
        <property type="entry name" value="BRCT_TopBP1_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18434">
        <property type="entry name" value="BRCT_TopBP1_rpt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17727">
        <property type="entry name" value="BRCT_TopBP1_rpt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17738">
        <property type="entry name" value="BRCT_TopBP1_rpt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17728">
        <property type="entry name" value="BRCT_TopBP1_rpt8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000018">
        <property type="entry name" value="DNA topoisomerase 2-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000028">
        <property type="entry name" value="DNA topoisomerase 2-binding protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000010">
        <property type="entry name" value="DNA topoisomerase II binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000020">
        <property type="entry name" value="DNA topoisomerase II binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000021">
        <property type="entry name" value="DNA topoisomerase II binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000023">
        <property type="entry name" value="DNA topoisomerase II binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000029">
        <property type="entry name" value="DNA topoisomerase II binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059215">
        <property type="entry name" value="BRCT2_TopBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049542">
        <property type="entry name" value="TopBP1-like_BRCT0"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044737">
        <property type="entry name" value="TopBP1_BRCT_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049936">
        <property type="entry name" value="TopBP1_BRCT_8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13561">
        <property type="entry name" value="DNA REPLICATION REGULATOR DPB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13561:SF20">
        <property type="entry name" value="DNA TOPOISOMERASE 2-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21298">
        <property type="entry name" value="TopBP1_BRCT0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="102"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="195"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="353"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="521"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="614"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="886"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="1243"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="16">
        <location>
            <begin position="1356"/>
            <end position="1453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="282"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="461"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="770"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1069"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1136"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1455"/>
            <end position="1484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="288"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="485"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1484" mass="163929" checksum="3D0F87213704C084" modified="2018-12-05" version="1">MSKAGKQDFIVKFVESEGSKPDYAVKAYEAILELQSEKYLKTIQEEDVLQMDRKDKSLFVFIKFTTPAFLHCKKLGCRIVSPLVVLDCLQQQRCVPKAEKPVYNMAMADVTISCTSLDKATRGEVMDLVQLMGGRVYLDLNVSVSHLIAGEVGSKKYLVAAKLGKPILLPTWVKACWEKSQDSLFRYTDLPVEDYLCPVLLGCIVCVTGLSSTERKEVQRLCEQHGAKYTGQLKMNECTHLIVSEPTGQKYECARKWNVYCVSLHWLFDSIEKGYSQDESRYMVQRKSSTTTQPNTSTPTGTSKREDGPSLLGLSNISVNTSAAINDTALTNITMSRMETPDPIDRLDLTVCPADDVLDGCKLYLSGFPEKNLEKLRRLVSAAGGLRFNQPREDLTHVIVREVDQELQKFLSTATHRPHVVTAQWLLDCFSKGMLVPEDGYLHPDCLPPASDAVAVSVSARPVARHRSSSGPPVANPSTPKEPITDEDLLSQYMDDDPTVEPQPESWTPLHTGGPEQEPLPQGSLFSGKSFLLVGFGVEAEAQLSALLKEQGGKLLLGRSRVVADFAVVPQLGCTVEATVDEVVTDTWLVMCVENERVLPLSSNPLFTPVPVREGYLPLKDCVLSVSQFTGAERESLVELAKHLGANVQDYFVRLANQKKGMLASTHLVLQTPDGTKYQAAKKWGLPAVTMHWILESARTGRRVTEEKYLVDLPSSPEKEDESFVSGSQKAAPLQPARCSPEIPLLGPQGGKAVTPLDCGRFQSKVFRSVLDKMKPRENPSTLTPNQDGGRGKPLQKEPSLQLDTPSRFLVRDQLFRPSFNLKVSTKKQTFSFILQGGVETPLTDFINRNLKVALANSSVNNATEMQEVSASPQLAKTIDKEIPQKDSGPLTGVVICVGKKLSKMQSELNAIAASLGADFRWACDNTVTHYIYQGRVGDNSREYKAVKEKGLHVVSQYWLHACAEEQKHVSESLYPFTYNPKMSLNLSQVPSSSQRSPPLTRTQLVHPVSNGCVDQDEVNDTTEKRGEDVFNIVDTLEMRENLQRQLQEIMSATKMTTGRRASVRLARMGSGCTDSGPNPPEGSRAGRSGSRRTLEALRVPREAVTDINTEPSQSEQIVWDDPTAREERAKLADNFQWPGSPSQHSEPLAAAPPPTAENGPRFKDSMTDSELVEMAACDVINQQMGQKVVAPSSEDPEEDILTPQAPSIAFPLAKPLVAPEPQVSASWNLPEEENQPPRFQLSSLSPQERIDYSHLIEELGGIILDKQSFDPSCSHIIVGSPLRNEKYLAAMAAGKWILHRSYLEACRSVGHFIQEDDYEWGSCSILEALPSITSQQKRLALAAMRWRKALQGSSEHDGAFSGWTVMLNIDQSRESGFRRLLQSGGAKVLPSPSPSLYREASHLFADFSRLKPGDFRVDVAEATAQGVTCLKPEYIADYLMQEPTPPVELYCLTAAPPEEDPCTPSRKRKAAADPSKLKKSRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZN1</accession>
    <name>A0A3B5LZN1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Mesoderm posterior ba</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZN1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016627"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016842.1">
        <property type="protein sequence ID" value="ENSXCOP00000016627.1"/>
        <property type="gene ID" value="ENSXCOG00000012547.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063712"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001707">
        <property type="term" value="P:mesoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032525">
        <property type="term" value="P:somite rostral/caudal axis specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18938">
        <property type="entry name" value="bHLH_TS_Mesp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000047">
        <property type="entry name" value="mesoderm posterior protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040259">
        <property type="entry name" value="Mesogenin/MesP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20937:SF18">
        <property type="entry name" value="BHLH TRANSCRIPTION FACTOR MESP-B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20937">
        <property type="entry name" value="IP14615P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="9">
        <location>
            <begin position="74"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="262" mass="29210" checksum="02EA07D3F33D9FCD" modified="2018-12-05" version="1">MNKYHRFWSSDTDLSSISSPETLSPVLSMDSSLSPNYQQPPQSTPKAVKCLKSSPCSLPGRGRKTGRNTRIRSKQRESASEKEKLRMRDLTKALHHLRSYLPPSVAPVGQTLTKIETLRLTIRYISYLSAQLGLSEEVLFQRREQVDTSASDASSPDLTSYFQNSSAGEHMQNQNPYQGLCYSQNRPLHSGHCSFGVNQLRMQYAEGPQGDMSVDAGLQSPPASQPSCQFVGKQLGTQLHSRSENKSHQKDITGHKMCVEHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0F5</accession>
    <name>A0A3B5M0F5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RNA binding motif protein 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0F5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013474"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013640.1">
        <property type="protein sequence ID" value="ENSXCOP00000013474.1"/>
        <property type="gene ID" value="ENSXCOG00000010216.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166798"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0L4</accession>
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    <sequence length="510" mass="55980" checksum="4CC52714A443C3C3" modified="2018-12-05" version="1">MPEMILTVSSSVEFKTPTRISRTKPSSVPGPNSPHNDPDLQQDIVWDAASPSPRRSGTRGEKAAAGGAVNISEIVSRIAPEVSSVWSEPALQQWIGDSAAIPCTPDPQEFNFGSVSCRATGVENLLRLARQLDQNLFQNQDLEEVLTEEGQGPRSFGSEPRPDRGFLQDDLDFLLDGPTQTRSRTPGQGQTQNRPGSASLCGSSPAGSGTCSGPGSVQNQLEDDWENDDLLNDSLVLEMTQNPDRFLVPKLCSTQNPAGSGQGWVAPSVGTRTEKENQDPTWNPDWSSGSGKWVQTRTEQNRFSSVFRAPEPGQMCSLEPESQWFWSDPMNRPGSDGPEVLGHLDSVFRSEPVWDGPSDDLLCEMCEDLENRIQVQTNRTGSDQRAALQPTHRNSGNRIRPDPAGQAGCSFAAAPVKQPIGRAVFRNRTGSEPLAALRPKDARCFTFRRPCDRVTMATEKGELELMMSFPVAAGKCSAAEIELKKQQALERRRIKLINNIYCYINKRVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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    </dbReference>
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            <begin position="1"/>
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        <source>
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    <sequence length="109" mass="11941" checksum="83B18BCBC0E24C3E" modified="2018-12-05" version="1">MGGSGSKVKGSWPFTGSGGDSGADGSEQSLARLKGSRNATPFVFTRRSSLYFDEDGDLAHEFYEETVVTKNGRRRSKLKRIQKNLIPQGIVKLDHPCIHVDFPIVLCEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3F6</accession>
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        </alternativeName>
        <alternativeName>
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        </alternativeName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">N4-methylcytidine (m4C) methyltransferase responsible for the methylation of position C839 in mitochondrial 12S rRNA. Involved in the stabilization of 12S rRNA folding, therefore facilitating the assembly of the mitochondrial small ribosomal subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>cytidine(839) in 12S rRNA + S-adenosyl-L-methionine = N(4)-methylcytidine(839) in 12S rRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:62524"/>
            <dbReference type="Rhea" id="RHEA-COMP:16109"/>
            <dbReference type="Rhea" id="RHEA-COMP:16110"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74506"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:62525"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. RsmH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3F6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014504"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014689.1">
        <property type="protein sequence ID" value="ENSXCOP00000014504.1"/>
        <property type="gene ID" value="ENSXCOG00000011000.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014756"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071424">
        <property type="term" value="F:rRNA (cytosine-N4-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.170:FF:000002">
        <property type="entry name" value="Probable methyltransferase-like protein 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.170">
        <property type="entry name" value="Putative methyltransferase TM0872, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01007">
        <property type="entry name" value="16SrRNA_methyltr_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002903">
        <property type="entry name" value="RsmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023397">
        <property type="entry name" value="SAM-dep_MeTrfase_MraW_recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00006">
        <property type="entry name" value="16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11265:SF0">
        <property type="entry name" value="12S RRNA N4-METHYLCYTIDINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11265">
        <property type="entry name" value="S-ADENOSYL-METHYLTRANSFERASE MRAW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01795">
        <property type="entry name" value="Methyltransf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81799">
        <property type="entry name" value="Putative methyltransferase TM0872, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017345326" description="12S rRNA N(4)-cytidine methyltransferase METTL15" evidence="14">
        <location>
            <begin position="21"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="376" mass="41577" checksum="6D32A25F6073C175" modified="2018-12-05" version="1">MPQQFFSLTSLMLRLNSATAGLSSGPGFWDRSDGLSCVSAEKPPHMPVMLKEVLHYLDVQPAQVVLDMTFGGGGHTKAILNMAPEVTVLALDRDPTAISLAQQLAKEHSGRVKPLLGRFSELEALLSNMNIKSGSIDAVLLDAGCSSMQMDQAERGFSLSKDGPLDMRMDGERYPDMPCAADVVNTLDQQALASILTAYGEERHARKIASAIVEARRVNPITRTQQLASVVAGAFPPGVVYARKDRLQRRAHIATKTFQALRIFVNDELNELHTGLCVAQSALKPGGRLCVITFHSLEDRLVKRFLQGHDLSNLDQYHFSQNKQGSRKKKLVDEDSECNKSSYWFPVKRKVVSPEKDDVRENPRGRSAKLRIALRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3H0</accession>
    <name>A0A3B5M3H0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Nectin cell adhesion molecule 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nectin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3H0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017910"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018134.1">
        <property type="protein sequence ID" value="ENSXCOP00000017910.1"/>
        <property type="gene ID" value="ENSXCOG00000013493.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165364"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000298">
        <property type="entry name" value="Nectin cell adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052659">
        <property type="entry name" value="Nectin/PVR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47387">
        <property type="entry name" value="NECTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47387:SF1">
        <property type="entry name" value="NECTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="81"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="179"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="298" mass="33186" checksum="B8933D84B626C83F" modified="2018-12-05" version="1">IWEPVDGQRDNIAVYHPTYGQSFPNVAFKDRVIFLQNNLRIPSIRIKDLRMSDAGRYTCEYATYPSGNEQGTTTLIMLAKPKNSAKAVTVQAGSKSVMVAHCEAADGKPAATIKWLASVGGNHSTTSTNGPDGTVTVRSEYRLVPTAEDNGREVTCLVDQKTQDRPWTEVLKLSVEYPPTVTIDGYDHNWYVGRSDAVLFCHATANPEPTTITWTTASGSLPDTVVVEQNKLTVRKVDDAVNTTFTCEVKNKHGASRKTLTTFVIGESKQNHPNTQRRRKRQTPHLHFLQHNLMQKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M413</accession>
    <name>A0A3B5M413_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cytoskeleton associated protein 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M413"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018105"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018336.1">
        <property type="protein sequence ID" value="ENSXCOP00000018105.1"/>
        <property type="gene ID" value="ENSXCOG00000013649.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014757"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061863">
        <property type="term" value="F:microtubule plus end polymerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030951">
        <property type="term" value="P:establishment or maintenance of microtubule cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007051">
        <property type="term" value="P:spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000050">
        <property type="entry name" value="Cytoskeleton-associated protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045110">
        <property type="entry name" value="XMAP215"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12609">
        <property type="entry name" value="MICROTUBULE ASSOCIATED PROTEIN XMAP215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOG" evidence="5">
        <location>
            <begin position="88"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="318"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="882" mass="99511" checksum="1DA0A011EFB68D65" modified="2018-12-05" version="1">MPAKTAAPTKTGGKGSAEKLLASVRSLLLSVCVNQGAAGKKPATKGLKDDEDKSGPIFVLVPNAKEQRMKEEKQLKILKWNFITPRDEYVEQLKTQMAACFAKWLLDEMFHFDFQRQVKAIGVMIERLNTESEATISCLDLILKWITLRFFETNTTILMKVLEYLKLLFTMLNRENYHLTEYEANSFVPYLILKVGESKDVVRKDVRVILSMLCKVYPASKLFPFLMDGTKSKNSKQRAECLEELGCLIEGYGMNVCQPTPAKSLKEIAVHIGDRDTSVRNAALNTVVAVYNVCGEQVYKLIGNLSEKDMSMLEERIKRSAKKTPAAPVKQSTAEKTQREQPTNPNATFLRKPAQEDPGKLNQSRQNAHSDHPSIPKEFQLDLDMIEQDQRSVCELPDLVQHQLDELLEPVMIPEPKIRSISPHFDDLHSSTASTINFVISQVASGDIGTSIQALAQIDEVLRQEDKADVMSGHIDQLLIATIMQLRLINSTHLADDRVDKKDLIKLYSCIMGNMMSLFSMETLAREASIGVLKDLMHSLITLMLDSRVEDVEDGTQVIRSVNLLVIRVLEHSDQTNIFSALLVLLQDTLVSNAGSLKVSELVMKCLWRMIRFLPENINSLNLDRILLDVHNFMKVFPKEKLKQLKTDVPHRTLKTLCHTLCKLTGVKILDHLSMIENRNESELEAYLRRVVKHSGNLSGTKSDRGNEKSALGTDDRMSKAKVSDILSEIFKKIGSKENTKEGLTELYEYKQKYSDADLEPFLRNTSQFFQSYVERGLRVIESEREGKPRIQSSSGFSWFCTSASLVCVCVCVCKLHLDLQSCQTTESSGEVALEDLDHTPCPLSGNLQKPLFLRLFHHFQVFSPPNLLIKCAKRSPKDPKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M449</accession>
    <name>A0A3B5M449_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Apoptotic protease-activating factor 1</fullName>
            <shortName evidence="9">APAF-1</shortName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer. Oligomerizes upon binding of cytochrome c and dATP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M449"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018145"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018376.1">
        <property type="protein sequence ID" value="ENSXCOP00000018145.1"/>
        <property type="gene ID" value="ENSXCOG00000013671.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157710"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043293">
        <property type="term" value="C:apoptosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097190">
        <property type="term" value="P:apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000081">
        <property type="entry name" value="Apoptotic protease-activating factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.430:FF:000001">
        <property type="entry name" value="Apoptotic protease-activating factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.370:FF:000001">
        <property type="entry name" value="Apoptotic protease-activating factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000135">
        <property type="entry name" value="Apoptotic protease-activating factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000502">
        <property type="entry name" value="Apoptotic protease-activating factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.430">
        <property type="entry name" value="Helical domain of apoptotic protease-activating factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017251">
        <property type="entry name" value="Apaf-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041452">
        <property type="entry name" value="APAF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042197">
        <property type="entry name" value="Apaf_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002182">
        <property type="entry name" value="NB-ARC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048975">
        <property type="entry name" value="WHD_APAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22845">
        <property type="entry name" value="APOPTOTIC PROTEASE-ACTIVATING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22845:SF5">
        <property type="entry name" value="APOPTOTIC PROTEASE-ACTIVATING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17908">
        <property type="entry name" value="APAF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00931">
        <property type="entry name" value="NB-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21296">
        <property type="entry name" value="WHD_APAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037646">
        <property type="entry name" value="Apop_pept_activating-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00364">
        <property type="entry name" value="DISEASERSIST"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00114">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 9" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CARD" evidence="11">
        <location>
            <begin position="3"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="657"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="746"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="881"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="1005"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="1046"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="1091"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="1133"/>
            <end position="1174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR037646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1259" mass="142911" checksum="C613E50AC458FA6F" modified="2018-12-05" version="1">MALQERTRRSLLRLRHQLEQDIKPQDLMDHMISDGVITVDEEEKVRIKPTRKDQAAALIELLLRKDDRSFISFYNALVKETYYDLAKPLYQDLPGVSFNGRKGSFDSSETSVQIMLSEGGVPQRPVVFVSRPELLNQVRMKLDLLQKDAGGWVTVFGMAGSGKSVLAAEAVRDQRLIEECFPNGIHWLSIGQLDKSDLLVKIQSLCFRLEQNLDSQSQQRPPTSLDEAKERLRFLMLRRFPRSLLILDDIWDSSVLKVFDIHCRILLTTRNRSLTDSVSGMSSVEVESGLDDRKALEILALYTKMNQNALPVEARDIVKECKGSPLVVSLIGALLKDKPNRWDYYLRQLRQKQFKRIRKSSSYDYDALDQAMAASIEVLPDEHRELYKDLTVLQKDVKIPAKILCVLWDLEPEEVEDILQEFVNKSLLFVDNNSRLNLYYLHDLQLDFLVEQNRDQIESLHSKVVNQYNHHYREAAPTSGDEGRLYWIRFLTYHLAKAQMFQELYSLMFSLDWVRMKAKVMGPAPLINDYMEYGPILDKENSEVCSQFQEFLSLNGHHLEQRPFPDVVQLALSQPHSSAVYRQAREQALHPANAGKLYFELINKRSIENLSRLVIHPHLGSIYSACFSHDGTKIASCGASKTLKVFKSTSGEKLIEIQAHDEEVLCCTFSPDDHLLATCSSDRKVKVWNIQQGKLLRTFEEEHEEQVNHCEFTNMSQRLLLATCSNDSILNVKLWNLNKPSSQNTMFGHFEPVNHCCFSPDDAYLSTSSNDGTVKLFQVSAANEWKTIDVKSFLSESDDEDVLVKCSTWTADGKRIICAARNAALVFDVQTSDMLLDIKTNRLSTVQYCHACPASNLLALAFSNYAIELWDLEEKKKRADCSGHLSWVQQVQFSKDGSQLLSCSDDQTIRLWETEKVHTSSAVSLKRDSDVLFDGEEIIVSAADNCNRLQVRDGRTGSVLFQSQELPFRIRSTCMCRNPSAVVLGQENGNVQVLALPSGKLLATLLGHTKTVLHFQFCQNGQTLITSSEDTTIRVWKWKSGECKVLQGHKEQVRCFSLLSDSANDSRLLSWSFDGTVKVWDIESGEKLQDIKAHQGAILSCHVSPDRCLFATTSADKTTKLWSCESWQCVFTLRGHQDCVRSCRFSWDSKRLATGDDNGEIRIWSVKDGSLLKVCSQESKDATDSVHGGWVTDLHFSPNNSVLVSTGGYIKWWDVARGESLQTFYPTGSALKKIHVSPDFSTFVTIDNIGILYILKRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4H5</accession>
    <name>A0A3B5M4H5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4H5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018275"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018506.1">
        <property type="protein sequence ID" value="ENSXCOP00000018275.1"/>
        <property type="gene ID" value="ENSXCOG00000013763.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165434"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03161">
        <property type="entry name" value="TM4SF2_6_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000029">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048232">
        <property type="entry name" value="TSN6/7_LEL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF51">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="273" mass="29424" checksum="5D5928B3FF13B176" modified="2018-12-05" version="1">MQVEKQFPNKRINRAAADADKDPTRVPSFCLFWAAGLAIFTLGVWAQISLADYMTLSANRYPNAPLILLSTGAAVTAWGFLGCLGVAANLPCVLRAYGFFQLATLVAGLAAGLSGLFYREDIAGGFRSGLHRAVAGYTEDEGRADALDSLQRALECCGAEGWRDWLNSDWAIQHMTFLPAENGTSVSLPDSCCMRRKGCRNRPLLSDDNDRVAAAGIHPHGCFRKVFSLVNDNVFHIAATVLGLAFTQIGGIALACLLANKLTPRQHGRVVAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4K7</accession>
    <name>A0A3B5M4K7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">E3 ubiquitin-protein ligase Topors</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">RING-type E3 ubiquitin transferase Topors</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">SUMO1-protein E3 ligase Topors</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Topoisomerase I-binding RING finger protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Topoisomerase I-binding arginine/serine-rich protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Tumor suppressor p53-binding protein 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 21">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4K7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014904"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015090.1">
        <property type="protein sequence ID" value="ENSXCOP00000014904.1"/>
        <property type="gene ID" value="ENSXCOG00000011297.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064170"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032391">
        <property type="term" value="C:photoreceptor connecting cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008630">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16574">
        <property type="entry name" value="RING-HC_Topors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000136">
        <property type="entry name" value="E3 ubiquitin-protein ligase Topors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058746">
        <property type="entry name" value="Znf_RING-type_Topors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46077">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TOPORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46077:SF1">
        <property type="entry name" value="TOP1 BINDING ARGININE_SERINE RICH PROTEIN, E3 UBIQUITIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="27"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00079040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="90" mass="10494" checksum="D6A7A06919D82F8B" modified="2018-12-05" version="1">SDNPGPSLSQTPKGFAMSAEVSPDSKCPICLDVFHNMSYLDRCLHKFCFRCILEWSKNKPECPLCKQPFRSIYHKIYYNKFCIFIYITDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5U9</accession>
    <name>A0A3B5M5U9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Thrombospondin type 1 domain containing 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">THSD4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M5U9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016791"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017008.1">
        <property type="protein sequence ID" value="ENSXCOP00000016791.1"/>
        <property type="gene ID" value="ENSXCOG00000012666.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156594"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000005">
        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010909">
        <property type="entry name" value="PLAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF319">
        <property type="entry name" value="THROMBOSPONDIN TYPE 1 DOMAIN CONTAINING 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08686">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50900">
        <property type="entry name" value="PLAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017261037" evidence="6">
        <location>
            <begin position="32"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="domain" description="PLAC" evidence="7">
        <location>
            <begin position="837"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="108"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="884" mass="98940" checksum="99A6C8AAF7F705BB" modified="2018-12-05" version="1">MLPMSTWLSHLNVLSHFFALSCLSNISSVASVQVPQRTESQQEFRSEYGEIQGAWGTWGAWSTCSRTCGKGVQEQTRPCLPVYTQYPSRGAAVQPQQPGHVISALKPAAAPRHSGVRAFNNSSSRGELRREKQSRPGERRKVTHIVPGKYGRGSASPHLHRQKRSPAANSLQAFPRGHTNLRSHKLDASNNLHRFTRPYVHPRTQHPRNNQLWTSLYQPVSSNQAEVQTGSHTSSHQLHKERPYNPLPSSFCTGEHAHYRICNSNDCPPSSKDIRAVQCSSYNNQPFMGRLYKWEPFNEVSGGQHCELNCRATGFRFYVRQSDRVIDGTPCGQNETNLCVAGRCASLGCDEYLGSGKVVDKCGVCGGDNSTCRLVSGVFKHSLSKIGYHKIVEIPEGATRINVTEMVKSRNYLALRSRSGRSIINGNWAIDRPGKYEGGGTMFTYRRPNEISSTAGESFLAEGPTNEILDVYVSRKIIIYSKKKKKVLSSLMARPMQKFFSGFRNSIFRCVHRLNHVEVSDAFCESSSRPGPQEEACNLQPCPAFWDIGEWSECSKTCGLGMQHRQVLCRQVYANRTLNVHMSRCQHLERPETASTCQLKICSEWQIRSEWTSCSVPCGVGQRSREVRCVSNVGDFVPDEECNMNLRPTNVENCDMGACAKSWFYTEWGNRCSAECGMGVRTRSILCLTNHISSLPLEGCGSEQPADSQICNNGPCDNRIEWFTGPWSQCSAECGTGSQQRSVVCLVKSDEGFTVMPPYECSSLDRPLSQQSCNIKTCGAKWYHTDWSACSKTCEGGFRVREVRCLSDDMLSSEACDEQLRPADREDCSPEPCIPQIDLNCRDKYYNCNVVVQARLCVYDYYKTACCASCSRIAHKQKVHRGHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6E2</accession>
    <name>A0A3B5M6E2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Neuropeptide FF receptor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">G-protein coupled receptor 147</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">RFamide-related peptide receptor OT7T022</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027888490.1">
        <property type="nucleotide sequence ID" value="XM_028032689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6E2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019783"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020029.1">
        <property type="protein sequence ID" value="ENSXCOP00000019783.1"/>
        <property type="gene ID" value="ENSXCOG00000014758.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114153981"/>
    <dbReference type="KEGG" id="xco:114153981"/>
    <dbReference type="CTD" id="570189"/>
    <dbReference type="GeneTree" id="ENSGT01150000286926"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008188">
        <property type="term" value="F:neuropeptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000153">
        <property type="entry name" value="Neuropeptide FF receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005395">
        <property type="entry name" value="NPFF_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241:SF82">
        <property type="entry name" value="NEUROPEPTIDE FF RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24241">
        <property type="entry name" value="NEUROPEPTIDE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01570">
        <property type="entry name" value="NPFFRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 15" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="47"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="67"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00075893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="481" mass="53643" checksum="99FA25998F07932E" modified="2018-12-05" version="1">MEVLENMGEGAMETEGSAIMATLLNGSLDGSANFTNLTFLPYYQHSLYVAASYILAYSFIFLLCMVGNVLVCLIVMENRCMRTVTNLFILNLAVSDLLVGIFCIPTTLVDNLITGWPFSNTVCKMSGFVQGVSVSASVFTLVAIAIERFRCIVYPLQPKLTLLVAKVAIVSIWVLAVAIMCPAAIALTVEKVPFHYMVYNDDFNHTLPLYTCYENFANPHLRKVYTAVLFAHIYLVPLTVITLMYVSIGVKLCSSAVANREPQLGNITVQAGGRRHAQQVVSQKKIKVIKMLIMVALLFMLSWLPLWTLMMMADYGGLDRDQLDLLTSYSFPFAHWLAFSNSSVNPIVYGYYNKNFKRGFQAVCKTKPFCCVARLQCRMPQWGRRGRSAQALSEGTELRDGSNNHSNFLLGLRNRVHNDNKLSETPEINRSSRMGCMVVHKGRSLSDQGIEMVAIHKKSSNDEGSEKVSSLAVSVYQAWDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7S5</accession>
    <name>A0A3B5M7S5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7S5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019545"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019789.1">
        <property type="protein sequence ID" value="ENSXCOP00000019545.1"/>
        <property type="gene ID" value="ENSXCOG00000014686.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183079"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990246">
        <property type="term" value="C:uniplex complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036444">
        <property type="term" value="P:calcium import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16175">
        <property type="entry name" value="EFh_MICU3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
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    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039800">
        <property type="entry name" value="MICU1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294:SF10">
        <property type="entry name" value="CALCIUM UPTAKE PROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12294">
        <property type="entry name" value="EF HAND DOMAIN FAMILY A1,A2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="205"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="11">
        <location>
            <begin position="404"/>
            <end position="439"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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            <dbReference type="ARBA" id="ARBA00022946"/>
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            <dbReference type="ARBA" id="ARBA00023128"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="464" mass="53764" checksum="C7AAF2A4E645BFC5" modified="2018-12-05" version="1">MAAFRRAAALAGKLNPWAATKSEILGAPSLNGRRTRRCLTAGLCAAAGGVLALYFYNKMWSDRKRMMRRSISGLSLPYIPTVEAKEKVRGCKNKAASEFWFYVLQTRPFDFEDGDVYMSSHEHRFRMFSSVEYEGQLYMTPQNFIESVTMTILIMFFVCLCQELEKILSDTPPVWRGSSKLFRNLRERGIISYTEYLFLLCILTKPHAGFKIAFNMFDADGNQMVDKREFLVVLKKDSQEFVARSYWDVLRRSASQVLFSDLAERSDESITIDTTILVHFFGKKGKAELTFDDFYRFMDNLQTEVLEIEFLTYSKGMTTISEEDFAKILLRFTNVENISAYMENVRQCIPDEKGITFDEFRSFFQFLNNLEDFAIAMQMYNFASRSIGQDEFARAVYVATGLKLTRHLVNTIFKIFDVDHDDQLSYKEFIGIMKDRLHRGARGYKSVERASSFRSCLKKELAIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8D0</accession>
    <name>A0A3B5M8D0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSXCOT00000016240.1">
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        <property type="gene ID" value="ENSXCOG00000012116.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Znf_LIM"/>
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        <property type="entry name" value="CYSTEINE-RICH PROTEIN 1 ISOFORM X1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="5">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="87" mass="10251" checksum="99BC49D1F71AFA30" modified="2018-12-05" version="1">MSFCVLWRNFGEKKRSLGRDYHPLCLKCQRCNRQLTAGQHAEYDDKPYCTHCYMKMFGPRCTLSGHTKNTHAHSRTFVYAFKCLRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAE2</accession>
    <name>A0A3B5MAE2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAE2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020780.1">
        <property type="protein sequence ID" value="ENSXCOP00000020530.1"/>
        <property type="gene ID" value="ENSXCOG00000015385.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166432"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF114">
        <property type="entry name" value="PCDH2G3 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="684"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="27"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="135"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="239"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="352"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="449"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="14">
        <location>
            <begin position="573"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="798" mass="87598" checksum="422D1B62F396072F" modified="2018-12-05" version="1">KRRQKQRFISQRYPDIFCCAFFLLNVAHGDMSYSFPEEMKRGSVVGNIAKDLGLQTGALSTRRARIDTEESDKRYCDINLKSGELIVAERIDREELCSEKASCILKQELVLENPLELHRISLHIQDINDNSPQFKDDLITSEIHESAVRGARFVIEEAHDADVGQNSVQQYSLEKNENFILAANGNTVELVLDKELDREKQKEINLLLTALDGGSPQRSGTVVIHVTVLDANDNAPVFSQAVYKASLPENSPIGTVVITVSATDADEGVNGDVTYDFGHVTNEVMKIFNIDSKKGEICLGAAIDHEVSTSYEMRIKAKDGFGLSSYAKVIISITDVNDNAPVVSLKSLTNPIPEDTPSGTEVGIINVQDRDSENNRQVRCSIQENVPFKLVPSIKNYYSLVTTGQLDRELVSDYNITITATDEGSPPLSSSKTIQLSVADINDNPPVFEEQSYSAYVSENNKPGSSLCTVSARDPDWRQNGTVIYSLLPGEVNGASVSSYLSVNGDTGVIHAVRSFDYEQFRSFKVQVMARDNGSPPLSSNVTVSVFISDVNDNSPQILYPAPEGSSFMTELVPKAAHGGSLVSKVIAVDADSGQNAWLSYHIVKATDSGLFGIDLHSGEIRTQRDISESDSMKQNLIVAVKDNGQPSLSATCSMYLLISDNLAEVPELKDISYDEKNSKLTSYLIIALVSVSTFFLTFIIIILGVRFCRRRKPRLLFDGAVAVPGPYFPPNYADIDPTGTLRSTSNYDAYLTTGSRTSDFKFVSSYNDNTLPADQTLKKSPTEFAIIFGDSDDSLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MC07</accession>
    <name>A0A3B5MC07_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Catenin beta 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-catenin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027865662.1">
        <property type="nucleotide sequence ID" value="XM_028009861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MC07"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021788"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022052.1">
        <property type="protein sequence ID" value="ENSXCOP00000021788.1"/>
        <property type="gene ID" value="ENSXCOG00000016286.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114139760"/>
    <dbReference type="GeneTree" id="ENSGT00940000155471"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014704">
        <property type="term" value="C:intercalated disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21724">
        <property type="entry name" value="CTNNAbd_CTNNB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000015">
        <property type="entry name" value="Catenin beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013284">
        <property type="entry name" value="Beta-catenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45976">
        <property type="entry name" value="ARMADILLO SEGMENT POLARITY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01869">
        <property type="entry name" value="BCATNINFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ARM" evidence="5">
        <location>
            <begin position="151"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="5">
        <location>
            <begin position="193"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="5">
        <location>
            <begin position="235"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="5">
        <location>
            <begin position="277"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="5">
        <location>
            <begin position="319"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="5">
        <location>
            <begin position="400"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="5">
        <location>
            <begin position="442"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="5">
        <location>
            <begin position="489"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="5">
        <location>
            <begin position="594"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="783" mass="85780" checksum="162CA7FED4D97516" modified="2018-12-05" version="1">MASQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEDDLDNNQVLYEWEQGFNQNFSQDQVQDIDGQYAMTRAQRVRAAMFPETLEEGMQIPSTQYDATNPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDMETARCTAGALHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLSKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQDAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHSPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDLHNRVVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEVAEAIEAEGASAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEAMSWNETGDLGLDIGAQGEPLGYRQDDPNYRPFHSGGYAGDAMGMEPMMDHDLGGGHHPGQDYPPVEGLPDLGHAPELIEGLPPGDSNQLAWFDTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCE6</accession>
    <name>A0A3B5MCE6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCE6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021215"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021475.1">
        <property type="protein sequence ID" value="ENSXCOP00000021215.1"/>
        <property type="gene ID" value="ENSXCOG00000015873.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160265"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004994">
        <property type="term" value="F:somatostatin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000039">
        <property type="entry name" value="somatostatin receptor type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000586">
        <property type="entry name" value="Somatstn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001856">
        <property type="entry name" value="Somatstn_rcpt_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229">
        <property type="entry name" value="NEUROPEPTIDES RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229:SF77">
        <property type="entry name" value="SI:ZFOS-169G10.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00246">
        <property type="entry name" value="SOMATOSTATNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00589">
        <property type="entry name" value="SOMATOSTTN3R"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="236"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="91"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="417"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="486" mass="54174" checksum="8AD7C76C1BC08F7D" modified="2018-12-05" version="1">MELIQATQVSLVPQEGTTATWNNNSVLLYYRPLFSTVPYAPLPSISQAEESFLFNSSCLNSTKSTPTSLPGLAGIFIPLIYGIVCIVGLLGNTLVIHVIVNYTKNESVTNIYILNLAIADELFMLGLPFLAVQNALLSWPFGSLMCRVVMTVDAFNQFTSIFCLTVMSVDRYLAVVHPIRASWWRRPHVAKAISATVWLVSFVVVLPVVVFADVLKDDGNCSIVWPEPAEVWKTSFIVYTCTVGFFCPLLVISLCYLLIIVKVGPLPKRTQTTSSRRRRSERKITRMVVVVVAVFVFCWLPFYALNILNLLVVLPGDFRGLYYFVVVLSYANSCANPILYGFLSDNFKRGFRKALCRASRKVKNNEKAGTEVQRPTEEWGGIVLLSQKRITNMHGKYCGENDVLEEVVGAEGGLQMRERSRLSHKGQNSKAEDDGTSPVIQEANPELTPGDDALEIAHCKANKCQNRNSQTEEVTDKNSVLEISYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCT4</accession>
    <name>A0A3B5MCT4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LITAF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Late endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCT4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021350"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021611.1">
        <property type="protein sequence ID" value="ENSXCOP00000021350.1"/>
        <property type="gene ID" value="ENSXCOG00000015980.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155366"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098560">
        <property type="term" value="C:cytoplasmic side of late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098574">
        <property type="term" value="C:cytoplasmic side of lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF47">
        <property type="entry name" value="LITAF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="LITAF" evidence="9">
        <location>
            <begin position="29"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="114" mass="12877" checksum="74D5549A4B95AFE0" modified="2018-12-05" version="1">MKLSVRPEQRYFFENRRRHSSCCRGKPPPAFSASITVSPLGENPAQIMCPKCHRTVLSEVEYSSGLFTYLFCGGLFLCGFVFGCCLIPFCVDRLKDAKHTCPTCKTVLGVYRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCX7</accession>
    <name>A0A3B5MCX7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SH3 domain containing 19</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCX7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021472"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021734.1">
        <property type="protein sequence ID" value="ENSXCOP00000021472.1"/>
        <property type="gene ID" value="ENSXCOG00000016073.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155694"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF48">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="325"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="404"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="464"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="552"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="132"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="251"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="262"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="284"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="624" mass="68587" checksum="A1EAE1EAFF14C467" modified="2018-12-05" version="1">SSFPKASEVKRGVKVVERTAEHTSTEGCSQNAQRPVPRPRTKLNKNATKDEHQTLIRLGENCDAFQSFSRDVPTNKYLEELLEVFGERSDCEEGRYYVDQSYEKSLDEDADGTMSANNNHRNIRARIQAFESQTGTEEDNESQAATPDLPPRRATYRPPVASKPPATFKPNGPNNSSQNMFASQNSQNQEPLTRPKPPTKPVGHSVREDSLPFRLTRSDSVHEEEPAPFPPVPPVKPFKDFVSVRRLAPVGFVPPSGNKSLPPRPPPARTGPGRPAPPSLQAVGRSQSEPQRSSPKFKAQMPTKKGLVLPPRPSPGHRLYNNYMLPLPHGIASADFDGRNSEELTLQKNEVVLLLEKISHSEFDCQIGETRGRVHKSRMTIITPLDADFSHPQEEGAAASGGGADGLKVKALHDFVPEGPMELSLSAGDVVTEVEQVDSQWYRGTCNGSTGFFPISYVKVLFCWVLNLCVVLFEGEHSDELSFSEGDVIQLKEYVGEDWARGQVGASVGIFPLNFVEVIEDLPPPPRQQPGPAARVALPGEDDSHLMLTAGSGVQWAVALYDYAAKTQDELSFQKDDCILITEHLSEEWSSGRLNGREGMFPRAFVNVSSGTESSQQGVKSSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MD94</accession>
    <name>A0A3B5MD94_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">REM-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MD94"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017674"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017898.1">
        <property type="protein sequence ID" value="ENSXCOP00000017674.1"/>
        <property type="gene ID" value="ENSXCOG00000013324.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157470"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13249">
        <property type="entry name" value="PH_rhotekin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012966">
        <property type="entry name" value="AHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051364">
        <property type="entry name" value="Cytokinesis/Rho-signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21538">
        <property type="entry name" value="ANILLIN/RHOTEKIN RTKN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21538:SF21">
        <property type="entry name" value="RHOTEKIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08174">
        <property type="entry name" value="Anillin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="REM-1" evidence="4">
        <location>
            <begin position="30"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="300"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="501"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="621"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="503"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="626"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="644"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="654" mass="72579" checksum="2BE61898B9E16407" modified="2018-12-05" version="1">MEDKRDIQTSRRHGSSKSVLSAGSAMAMEIKRKKMRESSFFLQKESTNIEEKVQFETRMREGAYKLLLACSKREQVLNASKNLLTCNARIKAYLAQLQREEGERDRGRSPCPGTVAVTGLRFPLIWRDSDHFSNRGSSRRVAVFCLMQIGSEVFDTEMVVADRSVTDICFEGLTVFKDVVPQFELKVELWSCSMEEELTLPNTPKKLAKKLRNSFGRSSGKKLCSLLDTPDPDTFLLDNPIPLGAKYSLLACTTLGLPDADSSFQSHSLIVYQGAEWSSWLPLYGNLCCRLVAQPACMTQSAMSGYLSQKQSVQGVNRCCNLYCLLSAGFLSCYFTPEEIDAKVQPTLNVPINNETRICVLDKQSGDRKSKSLSIINPSPEGSQSVVFTADTREELEEWIDCLNQHLYDQSQWLHCCDRLMKIEVASPRKPSLFLAKQADSVYNDLSINSPNKFESITDIIHSKIEETDGRFLIGQEEEREAPNWSSLFDGSHSVVVEKSLLSQSRTSTPCSSPSTGSTPLGNKKRRAPPPPSDVKPFANPTPVRSKTGRPSLDVKFSAIIQQLQRNNAGGVVAPSRKNAPLAVLDVQPQGPPQPSLQQFDIQNHSVQMPEDAADDVFVRAYSGPGPGPGSGPIPAPRSKLRKSLRERMNLKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDI6</accession>
    <name>A0A3B5MDI6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Arrestin domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Adapter protein that plays a role in regulating cell-surface expression of adrenergic receptors and probably also other G protein-coupled receptors. Plays a role in NEDD4-mediated ubiquitination and endocytosis af activated ADRB2 and subsequent ADRB2 degradation. May recruit NEDD4 to ADRB2. Alternatively, may function as adapter protein that does not play a major role in recruiting NEDD4 to ADRB2, but rather plays a role in a targeting ADRB2 to endosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027881591.1">
        <property type="nucleotide sequence ID" value="XM_028025790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDI6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021687"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021951.1">
        <property type="protein sequence ID" value="ENSXCOP00000021687.1"/>
        <property type="gene ID" value="ENSXCOG00000016214.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114149742"/>
    <dbReference type="KEGG" id="xco:114149742"/>
    <dbReference type="GeneTree" id="ENSGT00940000155411"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000007">
        <property type="entry name" value="Arrestin domain-containing protein 3 mRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF49">
        <property type="entry name" value="ARRESTIN DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0458">Lysosome</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="14">
        <location>
            <begin position="187"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="418" mass="46099" checksum="C5A2AEB452204C3B" modified="2018-12-05" version="1">MVLGKVKALAVYFDCLNENNLPVFSGGDLVSGRVVVEVTGDVRVKRLDITARGLAKVRWTESRNAGANTAYTQNYTEEVDYLHHCDTLIGEERDEDALEEGMTVLHAGLHEFAFSFNLPQMALATSFEGKHGSVRYWVKAELHRPWLLPVKVKKEFIVFEHIDINTPLLLAPQAGTKEKTLCCWFCASGPISLSAKIERKGYTPGESIQIFAEVENCSSRVVVPKAALYQTQTFFAKGKGRQIQQLVSNLRGEPLQQGKSQSWEGKLLKIPPVSPSILDCPIIRVEYALMVYVDIPGGLNLSLSLPLVIGTIPLHACATRTSSISSNCSSVSWMGLPERAEAPPSYSDLAISEAHRRNCLQGCDSSDGEGEDQGSVLTYITEFRYLPPPLYSEVDPYPDTVEVCGTADVRRPDMCPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDI7</accession>
    <name>A0A3B5MDI7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">UPF3B regulator of nonsense mediated mRNA decay</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RENT3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDI7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022328"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022601.1">
        <property type="protein sequence ID" value="ENSXCOP00000022328.1"/>
        <property type="gene ID" value="ENSXCOG00000016586.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017146"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12728">
        <property type="entry name" value="RRM_like_Smg4_UPF3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000067">
        <property type="entry name" value="regulator of nonsense transcripts 3A isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039722">
        <property type="entry name" value="Upf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005120">
        <property type="entry name" value="UPF3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034979">
        <property type="entry name" value="UPF3B_RRM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112:SF1">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13112">
        <property type="entry name" value="UPF3 REGULATOR OF NONSENSE TRANSCRIPTS-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03467">
        <property type="entry name" value="Smg4_UPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="UPF3" evidence="9">
        <location>
            <begin position="34"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="224"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="298"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="298"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="435" mass="52252" checksum="3608F40AF7C4AD31" modified="2018-12-05" version="1">MKEDKENTRPKDKRVEIKCEDGEKTEKCKEKKEAMTKIVIRRLPPSLSKEELEEQLQPLPEVDYMEFFSNDTSLFPHLFARAYISFKNQEDIVLFRDRFDGYVFIDNRGQEYPAIVEFAPFQKTAKKRNKKKDSKCGTIAEDPDYKRFLEYYNGDGEKLTSTPETLLEEIEARSKELEAKRTTPLLDFLKNKQRLREEKKEERRRRELERKRLRDEERRKWREEERRKRKEAEKMKRLEKPLENKDKDQTKEEPKIKVCVAEFQSRNWCYQTSLVQTAATKTGSGCFFLLGRGNEDGRKEFRERDLDREKERERRLREKERIRRQDEERRRRREQQDGENTSRKREEESRKEKAMEKRRSENSADADKMEKPARENKKEESSKRERLRNKDRPAIQLYQPGARSRNRTTRGGADSGSADRKPDSDNKKGADRGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEC0</accession>
    <name>A0A3B5MEC0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Clusterin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the clusterin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEC0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021895"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022162.1">
        <property type="protein sequence ID" value="ENSXCOP00000021895.1"/>
        <property type="gene ID" value="ENSXCOG00000016364.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063668"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000753">
        <property type="entry name" value="Clusterin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016015">
        <property type="entry name" value="Clusterin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016014">
        <property type="entry name" value="Clusterin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10970">
        <property type="entry name" value="CLUSTERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10970:SF2">
        <property type="entry name" value="CLUSTERIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01093">
        <property type="entry name" value="Clusterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00035">
        <property type="entry name" value="CLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00030">
        <property type="entry name" value="CLb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="402"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Clusterin N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Clusterin C-terminal" evidence="11">
        <location>
            <begin position="224"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="437" mass="50092" checksum="5217F5688B3B052B" modified="2018-12-05" version="1">AVFYHLDLLSEDGEKLVNEEMKRALYGVKQMREVMWRNEQKHEHLMNSLKHSSDKKKARELDVSEKLEEAEEQCKDSLQSEWEECRPCLEDACKTFYTSTCRRGFASFHSKVESFFQRVSRRFGPRERPLEAGDILVNQSPDESEPDVVQIENSFKRVMSKVGTLVNCSIALVSRMSSRLDGVLQKAFLNNTNELSEESASDPYYPGRDSGFLHGVGLEEVLESFFDFGKSVVEEFGAVVTQVFDDIHQTVEEETKKGNSKIKSFPRFLQNRRLCKDLRRQTSECWQLQNKCEACQGALLTECTGVRELHVELDEISQLLDISKQQYDEILSIVQHHTDETVKWLTNMAAEFSWVAQAVSNSSTPQNVFRITMVSRRTCGGQRCTNIKNNRHAYSLTTPANLYPAFFAVLIVPLQAILICAAEFIRYFLQSCKSHCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEU9</accession>
    <name>A0A3B5MEU9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myosin tail domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEU9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022364.1">
        <property type="protein sequence ID" value="ENSXCOP00000022095.1"/>
        <property type="gene ID" value="ENSXCOG00000016512.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154489"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150105">
        <property type="term" value="P:protein localization to cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349:SF2">
        <property type="entry name" value="CINGULIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349">
        <property type="entry name" value="CINGULIN-LIKE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin tail" evidence="4">
        <location>
            <begin position="517"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="649"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="161"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="259"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="672"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="683" mass="80087" checksum="27FD784A40B7FD8E" modified="2018-12-05" version="1">MFPNQVTPDLLKGQQELSADPPEEATKQILFTCLKNGTADDDPTTQRKVKLLLDNISELKSKAAESASATGIKALQEKRAALEKEVAHLKQKLGIEIKVQNNPSPRKKAFQIKANIDTVLRFGPSFTYNLLKFVKTRKSSKGATHCMKVKPFLCVCRFFRLTQMKTERERAKAEMKDLQQQLSEMHDELDRAKKTDVINTEKEVLLQDLAQMRVDFQEMLQVKEEQEEVLHHREMELSALKGALKEEVETHDKYIAALKEEYELELEKLLVHLQQAKEVRETFTKVQLNAYLGQEVAQVEEEKGAAKVQLKELIQERDNLKGKVRELSNKVEQLNQAIQEFKTTERMMEQRTKHSRLFLIQSKLTKLNHEHKDLKEKLKEERKQVEELWKMKAELEDERRMQDRAMEQLQRKMNTIMEECEASTDVLQHQVDEARERSQRELAELRRQLQEKGAELEKSRQAAKKLQEEVWHTLCSIQQYVLVSRMMSQRQGGEIKLILEHRTLHTKNKNYVSSVCLQDVECDKMSLERQNKDLKSRLSHLEGSQRTNQDSLVSKLNSRIQELEERLQGEERENNNLQQVNRKLERKVKEMKIQADEEHINLQSQMDQLTQRLKTAKRQMDEAEEEIERLEHTKKKLQRDVDEQMEANEQLHGQLNTLRKKKSPPFMKLVEEDLNDTDDFGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEX1</accession>
    <name>A0A3B5MEX1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sperm associated antigen 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEX1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022833"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023109.1">
        <property type="protein sequence ID" value="ENSXCOP00000022833.1"/>
        <property type="gene ID" value="ENSXCOG00000017062.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154697"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000221">
        <property type="entry name" value="Mitochondrial import receptor subunit TOM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051982">
        <property type="entry name" value="CiliaryAsmbly_MitoImport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45984">
        <property type="entry name" value="RNA (RNA) POLYMERASE II ASSOCIATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45984:SF3">
        <property type="entry name" value="SPERM-ASSOCIATED ANTIGEN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="287"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="321"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="47529" checksum="7054C5EB869504E7" modified="2018-12-05" version="1">LSFYLSPSLPLTSSSSGSTQPAYMGNAQKKPHSRGDGGPHTDTSNSHHGPWRGKGGTSDKYKVPEESKEKVANGSSKRGSTASAQGNKGTTGGEKAKETVNLDAPCGALPPHLSRLKNEGNLLFKNGQFADALEKYSQAIQGYTDSGMESPEDLCLLYSNRAACYLKDGNSQDCIQDCTRALELQPFSLKPLLRRAMAYESLERYRKAYVDYKTVLQIDTSVQAAHDSVNRITRMLIEEDGAEWREKLPDIPLVPLSAQQHRREEPPSQQVLQARAEKASRDAEVRFTALKQEGNDFVKKGQYQEALGKYAECLKLKPKECAIYTNRALCYLKLQRFGEAKQDCDAALELEPDNKKAFFRRALANKGLQDYLACSSDLQEVLQLDPNVQEAEKELEEVTVLLRQSLASATQDKPRKTVAITEVRLCSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHF7</accession>
    <name>A0A3B5MHF7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WSC domain containing 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WSCD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027889706.1">
        <property type="nucleotide sequence ID" value="XM_028033905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHF7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023050"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023327.1">
        <property type="protein sequence ID" value="ENSXCOP00000023050.1"/>
        <property type="gene ID" value="ENSXCOG00000017228.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114154624"/>
    <dbReference type="KEGG" id="xco:114154624"/>
    <dbReference type="CTD" id="9671"/>
    <dbReference type="GeneTree" id="ENSGT00940000159434"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051589">
        <property type="entry name" value="Sialate-O-sulfotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45964:SF7">
        <property type="entry name" value="SIALATE:O-SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45964">
        <property type="entry name" value="WSCD FAMILY MEMBER CG9164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="WSC" evidence="4">
        <location>
            <begin position="130"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="4">
        <location>
            <begin position="233"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="568" mass="64511" checksum="5E81DDE7CBB19D49" modified="2018-12-05" version="1">MAKPLLRIQRYFCRKPVRFFSLILLYLTAGSLVFLHSGFVGDSGPSTRGGRDSVVASEAGGRASSSDTRGLGIMSRVFKETRRSGRRFGPRWMRKSGQGGYKTAGRGGDYTSSWNRALKGRNAKDVEDGRARYIGCYVDDTQKRALRGVSFFDYKKMTVFRCQDNCAERGYMYAGLEFGAECFCGHKIQAPNASESECNMECKGERNNMCGGANRLSIYRLELSQESARRYGSAIFKGCFHRPDNVTAALPFSAVIQNMSVDKCVDMCTEREKSLAALAGEQCYCGFPTPLFSLHELEDEDMCLYRCPGEEFESCGNDKFFVVYQTQVQDNRCMDRHFLSARTKQLVALASFPGAGNTWARHLIELATGLYTGSYYFDGSLYNKGFKGERDHWRSGRTICIKTHESGKKEIEAFDSSILMIRNPYKALMAEFNRKYGGHVGFASQAHWRGKEWPEFVKNYAPWWASHTLDWLTYGKNVHVVHFEELKRDLFSHLKAMVQFLGLKVSEDRLLCVEGQKDGNFKRSGLRKLEYDPYTPDMRVNIDELIRTIDAALKKRNMSGVPAEYMPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHR8</accession>
    <name>A0A3B5MHR8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">116 kDa U5 small nuclear ribonucleoprotein component</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">U5 snRNP-specific protein, 116 kDa</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Required for pre-mRNA splicing as component of the spliceosome, including pre-catalytic, catalytic and post-catalytic spliceosomal complexes. Component of the U5 snRNP and the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHR8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023170"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023448.1">
        <property type="protein sequence ID" value="ENSXCOP00000023170.1"/>
        <property type="gene ID" value="ENSXCOG00000017309.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155685"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04098">
        <property type="entry name" value="eEF2_C_snRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04090">
        <property type="entry name" value="EF2_II_snRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01683">
        <property type="entry name" value="EF2_IV_snRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16264">
        <property type="entry name" value="snRNP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04167">
        <property type="entry name" value="Snu114p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000029">
        <property type="entry name" value="116 kDa U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000009">
        <property type="entry name" value="116 kDa U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000574">
        <property type="entry name" value="116 kDa U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1430.10:FF:000001">
        <property type="entry name" value="116 kDa U5 small nuclear ribonucleoprotein component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000033">
        <property type="entry name" value="Elongation factor Tu GTP binding domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1430.10">
        <property type="entry name" value="Yeast translation eEF2 (G' domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044121">
        <property type="entry name" value="Snu114_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035655">
        <property type="entry name" value="U5-116kDa_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF6">
        <property type="entry name" value="116 KDA U5 SMALL NUCLEAR RIBONUCLEOPROTEIN COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="2" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Tr-type G" evidence="7">
        <location>
            <begin position="5"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="817" mass="91712" checksum="31990B1E36AF8655" modified="2018-12-05" version="1">MDGTELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRDDVDLRYTDILFTEQERGVGIKSTPVTMVLPDSRGKSYLFNIMDTPGHVNFSDEVTSSMRISDGVVLFIDAAEGVMLNTERLIKHAVQERMAITICINKVDRLIVELKLPPTDAYYKLRHIVDEVNGMLSTYSTDENLVVSPLLGNVCFASSQYSICFTLGSFAKIYSDTYGDINYNEFAKRLWGDIYFNPKTRKFTKKAPTSNSQRSFVEFVLEPLYKILSQVVGDVDTTLPRVLDELGIHLTKEELKLNIRPLLRLVCNRFFGEFTGFVDMCVQHIPSPQEGAKIKIDHTYTGGLDSDLGEAMAECDPDGPLMCHTTKMYSTEDGVQFHAFGRVLSGTIQAGQPVKVLGENYTLDDEEDSQICTVGRLWISVARYQIEVNRVPAGNWVLIEGCDQPIVKTATITEPRGNEEAQIFRPLKFNTASVIKIAVEPVNPSELPKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVVTFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQTKYDWDLLAARSIWAFGPDTTGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLCDEPIRNVKFKILDAVIAQEPLHRGGGQVIPTARRVVYSAFLMATPRLMEPYYFVEVQAPADCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFALSVFHHWQVRFMDTNMQSAPSWWIGLLGVCLLKIKAWQVKIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MI10</accession>
    <name>A0A3B5MI10_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TAFH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MI10"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023265"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023543.1">
        <property type="protein sequence ID" value="ENSXCOP00000023265.1"/>
        <property type="gene ID" value="ENSXCOG00000017367.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011620"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08045">
        <property type="entry name" value="HFD_TAF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000015">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1110:FF:000002">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1110">
        <property type="entry name" value="TAFH/NHR1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045144">
        <property type="entry name" value="TAF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007900">
        <property type="entry name" value="TAF4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037249">
        <property type="entry name" value="TAFH/NHR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003894">
        <property type="entry name" value="TAFH_NHR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15138:SF18">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN ASSOCIATED FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15138">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05236">
        <property type="entry name" value="TAF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07531">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00549">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158553">
        <property type="entry name" value="TAFH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51119">
        <property type="entry name" value="TAFH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAFH" evidence="8">
        <location>
            <begin position="121"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="451"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="492"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="456"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="505"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="565" mass="61758" checksum="A9F82DB8CC99985B" modified="2018-12-05" version="1">FASGMVLVRSESGQLLVIPQQTLAQMQAQAQGGMAPRTATPTNVSAPGTTIISRQVSPGAIIRPGCPPPVSLAATTALHRAPILPVCRCSLLPGLAPRTLTPTPGTTVTSVTVSKVRSETMENVKKCKNFLSTLIKLASSGKQSTETAATVRELVKDLLEDKLEAEEFTSRLYKELNSSPQPYLVPFLKRSLPALRQLTPDSAAFIQQSQLTQPASVAVSSTSAMSTTVVLGPAPRLMTPPPGSRAQLQQTVSQGQTTSLVLQPQQQRAMLRPQVTLPTAPMMALRNQTPGRIMVGQQQVQLKELQPGEARDDDDINDVASMAGVNLSEESANILATNSGLVGAVTHSCKDEAFLSYALLQRRMLQIGMKYGVTELGTEVVNYISHATQQRLLNLLEKVSHVAQQKNINFKEDDSYEQSSDVRTQLKFFEQLDQLEKQRKEEQEREILLKAAKSRARQEDPEQLRLKQKAKEMQQQELAQMRQREANLTALAAIGPRKKKKNLDSPSSSASGSGTGSSLPGTVGSSGSRLTRQRITRVNLRDLLFCLENERFTSHSHFLYKGFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIY5</accession>
    <name>A0A3B5MIY5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. DCC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIY5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024293"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024584.1">
        <property type="protein sequence ID" value="ENSXCOP00000024293.1"/>
        <property type="gene ID" value="ENSXCOG00000018137.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159637"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000273">
        <property type="entry name" value="contactin-3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000551">
        <property type="entry name" value="Protogenin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000299">
        <property type="entry name" value="protogenin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000036">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF5">
        <property type="entry name" value="IMMUNOGLOBULIN SUPERFAMILY DCC SUBCLASS MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="820"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="31"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="133"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="223"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="319"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="417"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="512"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="622"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="720"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="908"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="979"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="908"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="979"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1015"/>
            <end position="1027"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1063" mass="116504" checksum="689C88007E57C74C" modified="2018-12-05" version="1">GSLQKPFETNQKCETRCLTSFNGLYIIAVFPCVGLSSFFAEPSSQTVTTGGSARFECQIEGVPTPVITWQKDKVTITDETRFISLPNGVLQILKVTKEDEGFYRCVASNSERKNISSEARLTVTTGSIDSPSPVVIVAPPQNATVVLGHPAVMECVARGQPKPLVSWSREDGEPISTDVIVLATNLVIRDTRRHHAGVYVCRANKPKTREFVFAAAELRVAAPPVIVQPPESVSLSRGNTARFLCNSSGEPFPVLHWLKNGQPIRSFRRMKVQSPGVLLINQLALEDAGYYQCIAENGLGTACAIARLTVIVKEGLPSRPRRLTATPYSSTTALLSWEKPEYNTDQIIAYSVHYQLTTGSDNEDSSLAVSNDTTEYLVKELLPHTVYTFFVVAYSLMGASPPSLPITIEMLEDVPSAPPQLSVASTSPTDIRLTWQPLTSQHSRGAVTRYRIDYKGTVFSVELRGNETQLTLRELRPNQVYRLRIAAGTAVGFGVPSEWVLQQTIVRNVIFAPSELKVRTRVTTLNVTWQPSPNHTLVSGYKLSYREAETDGFSIRDRTSHALTVRLRKKARHHLLTGLAPGRQYEVRVWAFDKQKDGAAAVWKGRTDKIHASGPSSSPPLPPSSIRATANGSTSIWLRWEKPRFINVRIINYTVRCSPAGTTNASLVSYYTSSSLEILLRGLRPFTHYELAVRSNGVNTAGPFSSTMVEATLPDRPSTPPEELQLRALNSSSVLVSWRPPLEPNGIIVSYKILYTVNLSEPEDEWRNLSEGGIFSVEVHGLSGGTRYFLKVGASTKVGSGPFSPVMEVQTPLSTYELDIHAVTGIIVGVCLGLICILLCMCFSFRNGKPRSVFTLYACFLFYTNGSRESLTESPKNNSSLIVLQETDDHLSPSLCSNQVEAEVVVHSELSDPPGREKEEGSERDSIATRGPSLSEDVYSPLSRPSSPREEAEHVEILSARGAESPPSLPARTLAAHHNEEAEGARQQENADREPQPDTCLTNGFHSPKTPRPMLKSEHLEMEDSRHCPSAAGKAISAGLPSAPFVSPGFVHSTSAAHIYLCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJE2</accession>
    <name>A0A3B5MJE2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA binding protein fox-1 homolog 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">RNA-binding protein that regulates alternative splicing events.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJE2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024459"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024753.1">
        <property type="protein sequence ID" value="ENSXCOP00000024459.1"/>
        <property type="gene ID" value="ENSXCOG00000018262.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159924"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12407">
        <property type="entry name" value="RRM_FOX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000004">
        <property type="entry name" value="RNA binding fox-1 homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025670">
        <property type="entry name" value="Fox-1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034237">
        <property type="entry name" value="FOX1_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017325">
        <property type="entry name" value="RBFOX1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047131">
        <property type="entry name" value="RBFOX1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15597">
        <property type="entry name" value="ATAXIN 2-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15597:SF25">
        <property type="entry name" value="RNA BINDING PROTEIN FOX-1 HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12414">
        <property type="entry name" value="Fox-1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037932">
        <property type="entry name" value="Ataxin_2_bd_A2BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="148"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="98"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="107"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with RNA" evidence="7">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR037932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037932-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="357" mass="38942" checksum="0A1D66DE9B83C728" modified="2018-12-05" version="1">GNTLCKSGSIAVFGNVVYSGLLNDHLWHLGVPLFTRLGNQEATNPPEAMAQPYTPAQYPPPPQNGIPAEFATPHPLPTQDYTGQSRVPEHAMTLYTPTQTHSEPAGTDNSTPAITATTTAPTDDVTQTEGSQQLQLQHSDSSEKQQPKRLHVSNIPFRFRDPDLRQMFGQFGKILDVEIIFNERGSKGFGFVTFETSTDADRAREKLNGTIVEGRKIEQVNNATARVMTNKKVANPYTNWKLNPVVGAVYGPELYATGFPYPATGATVAYRGAHLRGRGRAVYNTFRTAPPPPPIPAYGVVYQDGFYGAEIYGGYAAYRFAQPTTTAAYSDYGRVYATADPYHHTIGPAATYSVGTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MK40</accession>
    <name>A0A3B5MK40_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ADP-ribosylation factor GTPase-activating protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ADP-ribosylation factor 1 GTPase-activating protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">ARF1-directed GTPase-activating protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MK40"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024724"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025023.1">
        <property type="protein sequence ID" value="ENSXCOP00000024724.1"/>
        <property type="gene ID" value="ENSXCOG00000018468.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00890000139515"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030100">
        <property type="term" value="P:regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08830">
        <property type="entry name" value="ArfGap_ArfGap1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000008">
        <property type="entry name" value="ADP-ribosylation factor GTPase activating protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46395">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46395:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="20">
        <location>
            <begin position="7"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="127"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="307"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="334"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00071258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00077418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="385" mass="42545" checksum="6AD8C11D9CB349DD" modified="2018-12-05" version="1">MASPRTRRVLKEVRTQDENNLCFECGAFNPQWVSVTYGIWICLECSGKHRGLGVHLSFVRSVTMDKWKDIELEKMKAGGNGKFHLFLELQDDYDPNWTLQDKYNSKAAALFRDKVATLAEGKEWSIDTSPARNWTPPQPKSGLSSSHRSGGSGQNPASSGDKAFEDWLSDDVNSYQSGGGYSGNQENRYVGFGNTVTPEKKDEDFLNNAMSSIYSGWSSFTVGATKFASIAKDNTTKLANQATLKATELGQTINENVIKPTHEKVTLMFGVTKTTSDVNVLFPPLLASASSSSQPVSRDFWETFGSNSSLKEKKSPSSDSWTMADNSAKKSSDSWDNWGSEGASNNKHSTEESWEAWDNNWENADGKTKKSPSKPADEAWDNAGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKV0</accession>
    <name>A0A3B5MKV0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">F-box only protein 32</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO32) formed of CUL1, SKP1, RBX1 and FBXO32.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKV0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021766"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022030.1">
        <property type="protein sequence ID" value="ENSXCOP00000021766.1"/>
        <property type="gene ID" value="ENSXCOG00000016268.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004915"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040394">
        <property type="entry name" value="FBX25/32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13123:SF6">
        <property type="entry name" value="F-BOX ONLY PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13123">
        <property type="entry name" value="LD30288P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="11">
        <location>
            <begin position="215"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="17"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="348" mass="40720" checksum="8599AAD0CA5D632A" modified="2018-12-05" version="1">MPFLGQDWRSPGQNWVKTDEGWKRRTADDKNNNVSPECFNKENLLLSLSYEMSAKKRKKDLMNNNAKVPYFHREKWIYVHKGSTKERHGYCTLGEAFNRLDFSSAIKDTRRFNYVVRLLELIAKSQLPSLSGVAQKNYMNILERVVQKVLNDQQNVRPIKELLQTLYVSLCGLVQDMGKSVLVGNINIWVHRMDNILQWQQRLDNIQINRPAATGLALTDLPASLQLNIMQRLTDGRDLVSLGQVCPELGALTEDRLLWKKLCQYHFTDRQIRKRLMVSEKGHLEWKKMYFKLSRCYPHREQYSDTLHLCTHCHILFWKDTNHPCTANNPESCTTSLSPQDFIDLFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLI0</accession>
    <name>A0A3B5MLI0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Fibrillin 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">FBN1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fibrillin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000024628"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024926.1">
        <property type="protein sequence ID" value="ENSXCOP00000024628.1"/>
        <property type="gene ID" value="ENSXCOG00000018398.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183158"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001527">
        <property type="term" value="C:microfibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000038">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000058">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000086">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000097">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000007">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000009">
        <property type="entry name" value="Fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000003">
        <property type="entry name" value="Fibrillin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.290.10:FF:000020">
        <property type="entry name" value="Fibrillin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000003">
        <property type="entry name" value="fibrillin-1 isoform X1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000002">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000096">
        <property type="entry name" value="Putative fibrillin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000008">
        <property type="entry name" value="Signal peptide, CUB domain, EGF-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000119">
        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="21"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.290.10">
        <property type="entry name" value="TGF-beta binding (TB) domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040872">
        <property type="entry name" value="Fibrillin_U_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051145">
        <property type="entry name" value="GAS-SHBG-PROS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017878">
        <property type="entry name" value="TB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036773">
        <property type="entry name" value="TB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24040:SF16">
        <property type="entry name" value="FIBRILLIN-2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24040">
        <property type="entry name" value="LAMININ G-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18193">
        <property type="entry name" value="Fibrillin_U_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00683">
        <property type="entry name" value="TB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57581">
        <property type="entry name" value="TB module/8-cys domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLW2</accession>
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        <name type="scientific">Xiphophorus couchianus</name>
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    <sequence length="171" mass="18973" checksum="B74833A07ED7E9A7" modified="2018-12-05" version="1">YSVCLTTSTPHHQPAHQRPRLSAFLPRSRLSAFLPRSRLSAFLPRPRPSAFLPRPRPSAFLPRPQLSAFLPRPQLSAFLLRPRLSAFLLRPRLSAFLAASGRPRRPFVATFSAADDRRTASVVPMCVWVCAGVSVCMCGCVCAWLCVLSVSVLPCDRLATRQGDHASRPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLY0</accession>
    <name>A0A3B5MLY0_9TELE</name>
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        <recommendedName>
            <fullName evidence="8">Voltage-dependent calcium channel gamma-7 subunit</fullName>
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        <name type="scientific">Xiphophorus couchianus</name>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <text evidence="8">Regulates the activity of L-type calcium channels that contain CACNA1C as pore-forming subunit (By similarity). Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization and by mediating their resensitization. Shows specificity only for GRIA1 and GRIA2.</text>
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        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
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            <topology evidence="1 8">Multi-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="3 8">Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.</text>
    </comment>
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        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="term" value="F:channel regulator activity"/>
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        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:neurotransmitter receptor internalization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:neurotransmitter receptor transport, postsynaptic endosome to lysosome"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051968">
        <property type="term" value="P:positive regulation of synaptic transmission, glutamatergic"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:postsynaptic neurotransmitter receptor diffusion trapping"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:transmission of nerve impulse"/>
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        <property type="entry name" value="VDCC_gsu"/>
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    </dbReference>
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        <property type="entry name" value="Claudin_2"/>
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        <property type="entry name" value="VDCCGAMMA"/>
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        <property type="entry name" value="VDCCGAMMA7"/>
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        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0107">Calcium channel</keyword>
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    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0851">Voltage-gated channel</keyword>
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            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="129"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="290" mass="32754" checksum="7590CA8E9210C9BB" modified="2018-12-05" version="1">MSSFSTRALTLLSSVFGACGLLLVGVAVSTDYWLLMEEGIVLQQNQTMEVKMALHSGLWRVCFVAGPEKGRCVASEYFTEPEIEITTENTANILKMVRTATPFPMVSLLFVFTAFVISNIGHIRPQRTILAFVSGIFFILSGLSLVVGLVLYISSINDEVMNRPREPEQFFHYHYGWSFAFAASSFLLKEGAGVMSVYLFMKRYAEEELYRPHPALYRPRMSDCSDYSGQFLHPDSWAPPQRGRSPSDVSSDISIQLNQTPPPHEKHIYISTIKLSFENMRRGIISFLFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MME4</accession>
    <name>A0A3B5MME4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MME4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024953"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025257.1">
        <property type="protein sequence ID" value="ENSXCOP00000024953.1"/>
        <property type="gene ID" value="ENSXCOG00000018631.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183159"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030155">
        <property type="term" value="P:regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029978">
        <property type="entry name" value="LMO-7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46767">
        <property type="entry name" value="LIM DOMAIN ONLY PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46767:SF1">
        <property type="entry name" value="LIM DOMAIN ONLY PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="86"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="223" mass="25064" checksum="0E8FFDCA7BCD5A97" modified="2018-12-05" version="1">YQRSDSARLTSVIAPRPFGTQPSRISSLSRAHTDESHKGVNGNVDVSKKASVPSRYHQYMTSEDEAQSSSAHSSDEEEEEDFCEMRISLNQKPNSSRDFGFQAAWDSTGARVTSIQPSSAEMCQLQVRDEVLTVNGLRVAQMSYEQWKSSLEEALQKGSLVMDVRRHGQHPPETRTANTSLDFTSDVASNRVNGDFQDQPVTMRNKSEPISLKNLKRRSEFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNI7</accession>
    <name>A0A3B5MNI7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Splicing factor U2AF subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">U2 snRNP auxiliary factor large subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Necessary for the splicing of pre-mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the splicing factor SR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNI7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025974"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026285.1">
        <property type="protein sequence ID" value="ENSXCOP00000025974.1"/>
        <property type="gene ID" value="ENSXCOG00000019406.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155556"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12230">
        <property type="entry name" value="RRM1_U2AF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12231">
        <property type="entry name" value="RRM2_U2AF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12232">
        <property type="entry name" value="RRM3_U2AF65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001887">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000074">
        <property type="entry name" value="U2 snRNP auxiliary factor large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000097">
        <property type="entry name" value="U2 snRNP auxiliary factor large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006529">
        <property type="entry name" value="U2AF_lg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01642">
        <property type="entry name" value="U2AF_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23139">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="117"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="227"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="330"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="439" mass="49304" checksum="49DBF94BB533D2BB" modified="2018-12-05" version="1">DKENRHHRRSSSRSRSRERKRRSRDRDRRSRDRRGDSKERRHRRSRSPHREKKKKVKKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEESMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDVNLNDQAIAGLNGMQLGDKKLLVQRASVGSKNATLTTINQTPVTLQVPGLNSSVTQMGGLPTEVLCLMNMVAPEELLDDEEYEEIVEDVREECSKYGQVKSIEIPRPVDGLEVPGTGKIFVEFTSVFDSQKAMQGLTGRKFANRVVVTKYCDPDAYHRRDFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNX8</accession>
    <name>A0A3B5MNX8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNX8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025795.1">
        <property type="protein sequence ID" value="ENSXCOP00000025488.1"/>
        <property type="gene ID" value="ENSXCOG00000019043.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155177"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000191">
        <property type="entry name" value="Immunoglobulin superfamily member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000491">
        <property type="entry name" value="Immunoglobulin superfamily, member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051102">
        <property type="entry name" value="IgSF_V-set/TM_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207:SF25">
        <property type="entry name" value="IMMUNOGLOBULIN SUPERFAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017483511" description="Ig-like domain-containing protein" evidence="10">
        <location>
            <begin position="26"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="918"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="11"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="151"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="281"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="411"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="537"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="660"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="798"/>
            <end position="901"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="964" mass="108871" checksum="8A8DC6ACCA6FFBD4" modified="2018-12-05" version="1">MKSSVFSLWRPFLLILLGLFLLSEARVNTEIQAGPLYRVVGSLLSISCNTSGFSNVNSKKHFQIRMKDPSKPFHEINIMSSEDKGFSYARFIPRIQNSEMSIKHVDSNSIIFEIKYLKKEDEGVYECSVVNPEYNYNGVYSATTVVKVIDNSLSVTSSDAPTSPSYNEGEALSLTCQASTNTIQHVHLAFSWYIRKEGEQDAKPIISLDKDFTLVPGEGFEQRYRDKAIRLDKLDEATYRLNIAELEPLDQGQIYCQAQEWIQDPDRSWYKISQKDAEEIPLTVKAKVSLSPQNELHEGQELLLSCGIKAQNLAKRFFSVAWLWEGIELAKIGPTGILTVRQEYIDRERNGELKATRTGNNNYQLRVKPVKTTDKGSYSCRAWPEERGEDGNFNQGAAQDSQPVDITIFTPESGLSVKMENMKQPVSPGEKLKLICEADGVKGQLSVSWKRKATPPTSSLFATIISLNENGVTEKAEAFTSREVTVTRPTTNRFVFELADVADSDSGIYECIVSETQPSGRTQSQMQNVNVTVKSIDSVVKVVLRNRDPLVTMGNDVQLMCKVSSPRVPMTLTWSQETEDTNLNTIVVLYSNGSISWSGDQYRYQVQVDNQGNSVMHYLKVLRASHREEGKYQCMVSIFLHNVYKKLPASNLVNVKVQNPENKLALSPSHTIAQRINTDIQIDCKVTSRTSEFSLYAVTWELQQDAGNKTILTSDRNGITTFGQQIEESHRKRISMRHTDGPTFELTIQQARISDKGIYMCKVVEYLQDPWGEWYSLSDKHLTTVVDKEVEMNISIAEDFSIPCHITQQSSKESQFQVTWFWQQGTKVKTIFTAYRNSTLQARNKNVVVRYDHSSPHQFDLTVLKSNLENSGIYFCEVEEWIHAPADGWRKHAVEKSGNLNVTVDAEGKLSIPLTSTWITIYVITVSICLCIITILTCKLCRRKTSGGKNAGHSLWTEQVPLDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRI8</accession>
    <name>A0A3B5MRI8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SAM and SH3 domain containing 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRI8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022104"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022373.1">
        <property type="protein sequence ID" value="ENSXCOP00000022104.1"/>
        <property type="gene ID" value="ENSXCOG00000016515.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160111"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd09493">
        <property type="entry name" value="SAM_SASH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051725">
        <property type="entry name" value="SAM-SH3_domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021090">
        <property type="entry name" value="SPIDER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12301:SF5">
        <property type="entry name" value="SAM AND SH3 DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12301">
        <property type="entry name" value="SAM-DOMAIN, SH3 AND NUCLEAR LOCALIZATION SIGNALS PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12485">
        <property type="entry name" value="SPIDER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="163"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="6">
        <location>
            <begin position="243"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="78"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="300"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="112"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="229"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="307"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="323"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="350"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="393" mass="44086" checksum="EF6DA93CA6D27342" modified="2018-12-05" version="1">MLRRKPSNASEKEQTQKKKLTLQRSSSFKDFMKHKPTSPCTPDKEFTLDEHVKEEAVKSGSKLGKKWRNVISRTMTRKTSKMVQKALAEEGAESGEELSPVSSDFLPDLSAGQRTSVCSTGSEDTAPSPVSRQLSASNDRQSLDSGYCQRDSMRLEDSAYNGPFCGRAVVHTDFTPSPYDVESLKLQKGDIIHIIDKPPVGTWTGKLNNKVGSFKFIYVNLLPDETPPTRRKRCHSKGSRSKSKPKTLEEVLDRIGLTELSSLLSMHGFQSLEDFTGLKESHLNDLNITNPDHRSKILNAADLLRDSEDESEPEGDEEEEERPAENSKEPRDSGCFESSENLENGREEPKTEEEESNQQTEKEEEEKSEETQEAGDLEAVQEQLQELTVDEGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRT9</accession>
    <name>A0A3B5MRT9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">small monomeric GTPase</fullName>
            <ecNumber evidence="2">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRT9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027124"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027457.1">
        <property type="protein sequence ID" value="ENSXCOP00000027124.1"/>
        <property type="gene ID" value="ENSXCOG00000020250.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164158"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051065">
        <property type="entry name" value="Ras-related_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45704">
        <property type="entry name" value="RAS-LIKE FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="199" mass="22593" checksum="B4AF3E14A69C4C4F" modified="2018-12-05" version="1">MEANIVVMGTESVGKSMTVRLLTRRFIGEYGDISIYSQSFIVDGREVTLNIWDSPYSQDLSVEASHLHKKIQWADGFVLVYSICDRASFNAISRLVQKIKSTRDCFSRDKVPIVIVGNKKDLLHRRTVLSEEGRMLALSMDCLFYEVSAAENYHSVVSVFNGLVDRMKDAKLTTKRPLRFKGIVKSMSAVFARRRTDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MS09</accession>
    <name>A0A3B5MS09_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Diazepam binding inhibitor (GABA receptor modulator, acyl-CoA binding protein)</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ACBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MS09"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022274"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022544.1">
        <property type="protein sequence ID" value="ENSXCOP00000022274.1"/>
        <property type="gene ID" value="ENSXCOG00000016642.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154846"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310:SF62">
        <property type="entry name" value="ACYL-COA BINDING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310">
        <property type="entry name" value="ACYL-COA-BINDING PROTEIN, ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00689">
        <property type="entry name" value="ACOABINDINGP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACB" evidence="3">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="63" mass="7245" checksum="9863D566BC15704A" modified="2018-12-05" version="1">MLLLYSLFKQATVGDVNTARPGMLDFTGKAKWDAWEKQKGKSKEDSMNEYIKLVEELKQKHGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MS52</accession>
    <name>A0A3B5MS52_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Signal transducer and activator of transcription</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the transcription factor STAT family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MS52"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026326"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026645.1">
        <property type="protein sequence ID" value="ENSXCOP00000026326.1"/>
        <property type="gene ID" value="ENSXCOG00000019658.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244905"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.20:FF:000001">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.630:FF:000004">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000003">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.20">
        <property type="entry name" value="STAT transcription factor, all-alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.630">
        <property type="entry name" value="STAT transcription factor, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.532.10">
        <property type="entry name" value="STAT transcription factor, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001217">
        <property type="entry name" value="STAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048988">
        <property type="entry name" value="STAT_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036535">
        <property type="entry name" value="STAT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013800">
        <property type="entry name" value="STAT_TF_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015988">
        <property type="entry name" value="STAT_TF_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013801">
        <property type="entry name" value="STAT_TF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012345">
        <property type="entry name" value="STAT_TF_DNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013799">
        <property type="entry name" value="STAT_TF_prot_interaction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11801">
        <property type="entry name" value="SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01017">
        <property type="entry name" value="STAT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02864">
        <property type="entry name" value="STAT_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02865">
        <property type="entry name" value="STAT_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21354">
        <property type="entry name" value="STAT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00964">
        <property type="entry name" value="STAT_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47655">
        <property type="entry name" value="STAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48092">
        <property type="entry name" value="Transcription factor STAT-4 N-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0010">Activator</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12 13" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="10 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 13" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SH2" evidence="15">
        <location>
            <begin position="559"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="118"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU046415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="730" mass="84384" checksum="50E9348B9F037E25" modified="2018-12-05" version="1">MAQWDRLRQLSAIYWQQLHELYDRDGLPMDVRHYLSAWIEKQEWQRAARDYGLAMVLYQVLLENLDIQHSRFVQEESFLQQHNIRRYKQSFQRYQDEPCALASTIQWFLEKEKEILNSAELNEQVQLLQVEQEAMETTCQQELQRKMAGLRNDVQCLEHAIICLEEQQDEFDFKYQTHKMEAVTDEAQKNERKKMFQSLINKLNDYRKSTLADLNKLLDRAEDLINLLVRKELVDWQRRQQKSCIGAPDNVCLDQLEKWFTCVAVCLFQVREFLNKLDELVGKMSYENDPIKAQKPALNKRVDALLKDLLKSSFVVETQPSMPQGKGPLVLRTNVQFSVKTRLLVKFAELNHSMKVNVSMSRYRRFNVLGSATKDLNMAESLSGGMVADFRHLSLKEQKSGGGGKGISEISLSVTEELHIIYFNTVFELKGFSVELQASSLPVVIISNSSQQQSAWASILWFNMLSLDPKDIMFFTTVPAAPWPQFGEMLSWQFLSATKRGLDDDQRKMIAHKLFGKQQNYDICQVTWSKFSKESGPETFWVWFDGILVMVKSYLENIWREGLIMGFVSRGKEKLLLKKKKRGTFLLRFSESVVGGITFSWVEIDSTGEPDIKTVQPFTKTDLCQIPFHEIIRNFQILEAENIPESPLLFLYPDTPKDEAFGKWYCEKSGGMGFTSRSPMSCDMTQGDCLEPMNGLYGEAAGESWLSLLSNPAENLQTNQPHHQRALSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MTJ3</accession>
    <name>A0A3B5MTJ3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ANKRD34 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027889377.1">
        <property type="nucleotide sequence ID" value="XM_028033576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MTJ3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027083"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027416.1">
        <property type="protein sequence ID" value="ENSXCOP00000027083.1"/>
        <property type="gene ID" value="ENSXCOG00000020230.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114154474"/>
    <dbReference type="KEGG" id="xco:114154474"/>
    <dbReference type="CTD" id="390616"/>
    <dbReference type="GeneTree" id="ENSGT00390000012355"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042637">
        <property type="entry name" value="AN34A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24156">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24156:SF6">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN 34C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="42"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="83"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="218"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="316"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="491"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="316"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="403"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="503"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="530" mass="57486" checksum="B01B39A6054B5BDE" modified="2018-12-05" version="1">MADILELRTDGNSLLKAVWLKRLRLTRLLLEGGAYINESNERGETPLMVACMSTHSDQQSVTKSKLVKYLLDNQADPNIQDKAGRTALMHACIHKAGHEVVDHLLSNGADPSLEDRSGASALVYAINADDKETLKLLLDACKAKGKEVIIITTDKSPSGTKTTKQYLNVPPSPELIERSSPEYCASPSDVNVTASSTSEQEQQNTVFSFQTKLKTSVAASKLTNGPTSPTRRPANPKRARLPQLKRLQSEPWGLIAPSVLAAAAAHEESKKASSDEDVVAGVNGLSLNKRSALTRQSSVDGKDSLFPLVVDQPCKMTTSLSVPPTSKQSYERSLGQHQPLARRSTVPTEQENGSSSSSGQVSLRDTLHRRRLGNDHYDSDSQLYSDSAMLDSPKVPVERRKLNTSPLAMLTSSRESLDSNASTSSPSTARRRPPGLLERRGSGTLLLDYISHTRPGQLPPLNVNPNPPIPDIGASNKPSSPLLAGIKSIAPVAPNTPKTGGLKSKKKLARRHSMQVEQMKQLYDFEEPPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVS9</accession>
    <name>A0A3B5MVS9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVS9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027701"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028036.1">
        <property type="protein sequence ID" value="ENSXCOP00000027701.1"/>
        <property type="gene ID" value="ENSXCOG00000020654.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000272264"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="111" mass="13009" checksum="045EC22F0EAEA3A2" modified="2018-12-05" version="1">IILSLGQLLSFLIKLALEKQELEWEKVQLTGILPYCALVFIAVFDTHNNNITFQGKYQVSAELKDKEINNLKEELKSLQVLRSSAFSLFFFLCFPSRRKSLYFFFSPFIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWC7</accession>
    <name>A0A3B5MWC7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWC7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028326.1">
        <property type="protein sequence ID" value="ENSXCOP00000027986.1"/>
        <property type="gene ID" value="ENSXCOG00000020881.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165017"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="125" mass="14047" checksum="EE0D4885AB12FF92" modified="2018-12-05" version="1">ILNYKTRGGKGQHDHCFGTLEFQELISEVYPLSQFEHGADDVLILGTDGLWDVLSNQEVAEAVTGFLANCDPDDQHRYTMAAQDLVMRARGELKDRGWRISNDRLGSGDDISVYIIPLMYGNKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWH5</accession>
    <name>A0A3B5MWH5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large neutral amino acids transporter small subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">L-type amino acid transporter 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Solute carrier family 43 member 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-isoleucine(in) = L-isoleucine(out)</text>
            <dbReference type="Rhea" id="RHEA:70943"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-leucine(in) = L-leucine(out)</text>
            <dbReference type="Rhea" id="RHEA:73011"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-methionine(in) = L-methionine(out)</text>
            <dbReference type="Rhea" id="RHEA:70939"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-phenylalanine(in) = L-phenylalanine(out)</text>
            <dbReference type="Rhea" id="RHEA:27950"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SLC43A transporter (TC 2.A.1.44) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027887967.1">
        <property type="nucleotide sequence ID" value="XM_028032166.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027887968.1">
        <property type="nucleotide sequence ID" value="XM_028032167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWH5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024304.1">
        <property type="protein sequence ID" value="ENSXCOP00000024019.1"/>
        <property type="gene ID" value="ENSXCOG00000017929.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114153548"/>
    <dbReference type="KEGG" id="xco:114153548"/>
    <dbReference type="CTD" id="494042"/>
    <dbReference type="GeneTree" id="ENSGT00940000153576"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015175">
        <property type="term" value="F:neutral L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20766:SF2">
        <property type="entry name" value="LARGE NEUTRAL AMINO ACIDS TRANSPORTER SMALL SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20766">
        <property type="entry name" value="LARGE NEUTRAL AMINO ACIDS TRANSPORTER SMALL SUBUNIT 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="442"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="533"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="587" mass="65194" checksum="033DEC08CB248CA9" modified="2018-12-05" version="1">MAPTLATAFARRWWMAVSAIIENLLFSAVLLGWGSLLIMLKSEGFYSYLCNEEGNSSSYNLSHSAANSVPHDYADAFEPDESDGVEMKPLVPEGPLRMNGWLICKEQDEMLNLAFTVGSFLLSAITLPLGIVMDKYGPRKLRLLGSTCFGFSCLLIAYGAYQPNELSVLIFIALTFNGFGGMCMTFTSLTLPNMFGNLRSTFIALMIGSYASSAVTFPGVKVIYDFGASFITILVVWASCAGLVFLNCFFNWPLEPFPGPEDMDYTVKIKFSWLGFDHKITGKQFYRQVTTVGRRLSVSNNVKQKEVVGKDGRTLCLSTMDLEMDCKPKEESESFLKSIMSPIFMLSASTMCITQLRLLFYMGAMNSVLEVLCDGNLDTVSLYSSIFGVLQLLCLMTTPVIGQIMDWKLKDCDDGEEDKEPLSKGESKTKRRDRKIQKVTNALRAFVLTNLLLVGFGVTCLIPNLQLQIVSFILHTVVRGFIHSAVGGLYAAVYPSSQFGSLTGMQSLVSAVFALLQQPLFLAMMGPLEGDPFWVNIGLLVLSMLGFLLPLYLIWYRRTLHRELQQKAEDAKIYIKINGSEIPEAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXI6</accession>
    <name>A0A3B5MXI6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S26</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS26 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXI6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026372"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026692.1">
        <property type="protein sequence ID" value="ENSXCOP00000026372.1"/>
        <property type="gene ID" value="ENSXCOG00000019693.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002517"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1740.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.20">
        <property type="entry name" value="Ribosomal protein S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000892">
        <property type="entry name" value="Ribosomal_eS26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047864">
        <property type="entry name" value="Ribosomal_eS26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038551">
        <property type="entry name" value="Ribosomal_eS26_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538:SF10">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01283">
        <property type="entry name" value="Ribosomal_S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00733">
        <property type="entry name" value="RIBOSOMAL_S26E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="114" mass="12851" checksum="97A3BC1E66968320" modified="2018-12-05" version="1">MTKKRRNNGRAKKGRGHVQPIRCTNCARCVPKDKAIKKFVIRNIVEAAAVRDISDASVFDYVLPKLYVKLHYCVSCAIHSKVVRNRSCEARKDRTPPPRFRPAAGAPRAPPKPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXY4</accession>
    <name>A0A3B5MXY4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BCL10 immune signaling adaptor</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXY4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025279.1">
        <property type="protein sequence ID" value="ENSXCOP00000024975.1"/>
        <property type="gene ID" value="ENSXCOG00000018643.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165852"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032449">
        <property type="term" value="C:CBM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019209">
        <property type="term" value="F:kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051059">
        <property type="term" value="F:NF-kappaB binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043422">
        <property type="term" value="F:protein kinase B binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001238">
        <property type="term" value="P:positive regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08810">
        <property type="entry name" value="CARD_BCL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033238">
        <property type="entry name" value="BCL10/E10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042143">
        <property type="entry name" value="CARD_BCL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34920">
        <property type="entry name" value="B-CELL LYMPHOMA/LEUKEMIA 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34920:SF1">
        <property type="entry name" value="B-CELL LYMPHOMA_LEUKEMIA 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CARD" evidence="2">
        <location>
            <begin position="11"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="241" mass="26312" checksum="A1F5AD350EE7AA7C" modified="2018-12-05" version="1">MDVSHLTEDEMAEIKKDVLTRLRHYLCDKIRADRHLDYLRSRRILTRDDAEEISCRTTQTKRTATLLDILAENPRGLDALIDSIRELRSQNFIITKITDEVQKAKNEKIESLRGHTLTTTTDLPNTFSNNSTLLFHPDGERSPSTSDLASSLNMSSLQKCGDLPSAAGASLGVAPSSTSSSLPKPGDPGAPPLPDEVMAESPSNIDTTSPGCTSSGGDPNFQPLRSRSLTPTIHIPQFTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MYK4</accession>
    <name>A0A3B5MYK4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome c oxidase subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome c oxidase subunit 6B.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027891197.1">
        <property type="nucleotide sequence ID" value="XM_028035396.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027891198.1">
        <property type="nucleotide sequence ID" value="XM_028035397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MYK4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025420"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025727.1">
        <property type="protein sequence ID" value="ENSXCOP00000025420.1"/>
        <property type="gene ID" value="ENSXCOG00000018989.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114155485"/>
    <dbReference type="KEGG" id="xco:114155485"/>
    <dbReference type="GeneTree" id="ENSGT00940000164148"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00926">
        <property type="entry name" value="Cyt_c_Oxidase_VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.140:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 6B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.140">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048280">
        <property type="entry name" value="COX6B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036549">
        <property type="entry name" value="CX6/COA6-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003213">
        <property type="entry name" value="Cyt_c_oxidase_su6B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11387">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02297">
        <property type="entry name" value="COX6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000278">
        <property type="entry name" value="Cyt_c_oxidase_6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47694">
        <property type="entry name" value="Cytochrome c oxidase subunit h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="30"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000278-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="86" mass="10393" checksum="F876324366603585" modified="2018-12-05" version="1">MSVNFEEKIKKYRTAPFDARFPNTNQTRNCYQNYLDYHRCNKVLSAKNQKTTPCEWYRRVYKSLCPMAWVQKWDEQLQSGTFAGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N0B9</accession>
    <name>A0A3B5N0B9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5N0B9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026495"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026816.1">
        <property type="protein sequence ID" value="ENSXCOP00000026495.1"/>
        <property type="gene ID" value="ENSXCOG00000019780.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156521"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000114">
        <property type="term" value="P:regulation of establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13243">
        <property type="entry name" value="PH_PLEKHG1_G2_G3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000019">
        <property type="entry name" value="Pleckstrin homology domain-containing family G member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043324">
        <property type="entry name" value="PH_PLEKHG1_G2_G3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45924">
        <property type="entry name" value="FI17866P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45924:SF4">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY G MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="3">
        <location>
            <begin position="127"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="422"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="43"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="428"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="477" mass="53693" checksum="09CF0AB9CA0903E6" modified="2018-12-05" version="1">RLAGRLTDQSPRLSIASISSDERAPSATPSDPSDPPASGQRPLSLISTLSSGSGSSRDDYVAPPPESEASFGVDLNQNPSPEKRGRSFVHNNNNNNKASTPSRASSRRQPALPFVGATMVPDPKLKYLDRIVMEIIETERMYVRDLKIIVEDYLAHVIDESDLSNQPKLVCDLFGNIEDIYEFNSELLQDLERCDNDPVAIASCFVSKSEDFDIYTQYCTSYPESVEALTKCMNNISVAKFFRDRQASLKHSLPLGSYLLKPVQRILKYHLLLQEIAKHFGPQKEGYDVVEEALYTMTWVAWYINDMKRKHEHAVRLQEVQSLLVNWKGPDLTTYGELVLEGTFKVPRARHERTLFLFDRMLLITKRRGDYHGNGPRSATPASFSVSHYKHLKQTHNAKTVEEKKLWAHHIKRIILENHQAAIPQKVSGSDSSTTSKTSTESQQLPALSPRCQRPEPQRSAGPEVRPAPPDASGNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N166</accession>
    <name>A0A3B5N166_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Family with sequence similarity 107 member B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027897859.1">
        <property type="nucleotide sequence ID" value="XM_028042058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5N166"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027230"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027563.1">
        <property type="protein sequence ID" value="ENSXCOP00000027230.1"/>
        <property type="gene ID" value="ENSXCOG00000020336.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114159846"/>
    <dbReference type="KEGG" id="xco:114159846"/>
    <dbReference type="CTD" id="83641"/>
    <dbReference type="GeneTree" id="ENSGT00390000011228"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009533">
        <property type="entry name" value="FAM107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16768">
        <property type="entry name" value="DOWN REGULATED IN RENAL CARCINOMA 1/TU3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16768:SF1">
        <property type="entry name" value="PROTEIN FAM107B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06625">
        <property type="entry name" value="DUF1151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="118"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="157" mass="18499" checksum="6B5CADB433634E05" modified="2018-12-05" version="1">MATMFRYPVFPHLQDPCDPGRSVMAEPDYLEGDCDELIQPKKLINPVKSSRNHQDLHRELIMNQKRGLAPQNKPELQKVLEKRKREQVIKAQKEEQEAHKKRSDLEIELMKRQQKLEQLELEQQKSQEEQENTPEFVKMKGNLRRTKQEAGGEEQAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KLG6</accession>
    <name>A0A3B5KLG6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Xrn1 helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'-3' exonuclease family. XRN2/RAT1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KLG6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000948"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000960.1">
        <property type="protein sequence ID" value="ENSXCOP00000000948.1"/>
        <property type="gene ID" value="ENSXCOG00000000783.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00670000098098"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.1050:FF:000002">
        <property type="entry name" value="5'-3' exoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF41">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xrn1 helical" evidence="7">
        <location>
            <begin position="4"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="237"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="285"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="298" mass="32949" checksum="D6289D771516362E" modified="2018-12-05" version="1">MFSEFEKDTKPFKPLEQLMGVFPAASGNFLPPTWRNLMMSADSSIIDFYPDDFAIDLNGKKYAWQGVALLPFVDERRLRAALADVYPDLTPEEGETRSPHLVGRGHLVGRAALRLFQEAPVPAELCHGIQGTLNLDDNPILPDETVKSPVPLLRDISLNSSIKDPQFAEGFVFKAVLLPGAKMPSKVLKPEDYERGARGPWRPQLGFNPNRQQAHLDQAGFRALGVWAAERLRARCSRPAQRPWTRSERTRETNLGSWADCRAGQAARAQRAGRQAPPRSGPAAPAAPPPRHGPRPGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KNQ5</accession>
    <name>A0A3B5KNQ5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Myelin transcription factor 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYT1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KNQ5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000108.1">
        <property type="protein sequence ID" value="ENSXCOP00000000107.1"/>
        <property type="gene ID" value="ENSXCOG00000000085.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156364"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.320.30:FF:000001">
        <property type="entry name" value="Myelin transcription factor 1-like, a"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.30">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013681">
        <property type="entry name" value="Myelin_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002515">
        <property type="entry name" value="Znf_C2H2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036060">
        <property type="entry name" value="Znf_C2H2C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816:SF10">
        <property type="entry name" value="MYELIN TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10816">
        <property type="entry name" value="MYELIN TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08474">
        <property type="entry name" value="MYT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01530">
        <property type="entry name" value="zf-C2HC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103637">
        <property type="entry name" value="CCHHC domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51802">
        <property type="entry name" value="ZF_CCHHC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Myelin transcription factor 1" evidence="14">
        <location>
            <begin position="461"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="140"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="507"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="577"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="854"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="77"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="210"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="511"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="609"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="947" mass="105016" checksum="A669E71902FE64F1" modified="2018-12-05" version="1">EAEQEQEQEAEKPASKRKSHPLKLALDEGFSAESDGSSEAEEEAEKDGGKVEKAVTGSGEEEEKDGGAEEEEIEAEPLEKDADGLMKGKEEEEEEKETQQQEEKTSSPEEVADSLLHLGRVSNGHAVGVVVQQIEDVRAAAEQGEEAKHQQEGAQRSPEESSVLQNEARKVEEEQGERVNMSNHVSRGKQIKGEKEEEMVVSVQQTQETPTDDEEDDEKDEEEQRRPFLSISDVPTANYKLHKASSSASPDVIERDDEDSLSQRSTVTDESEMFDMTRGNLGLLEQAIALKAEQVKPAGPRELLRAPDLHHQRYFTIEDRPKHLDIIRKTYFKRPEKRENKCPTPGCDGTGHVTGLYPHHRSLSGCPHKDRIPPEILAMHENVLKCPTPGCTGQGHVNSNRNTHRSLSGCPIAAAEKLSKTHDKQHLAQPGSELLKGSPNDRVLRPMCFVKQLEVPQYGNYRPNMAPSTPRANLAKELEKYSKVSFDYASFDAQVFGKRTLAPKMTTSETSPKAFKTKPSFPKSSSPTLSLHAYGKSATLPYDYSHDAEAAHMAATAILNLSTRCWEKPENLSIKPQTKEMDIEVDENGTLDLSMKKPIKREGSVSGTSPGVRSPDPSSSSSSSFHHSGNSGMTSPNLHTYKQEDWEGPLDFTKPNRQREEETDEMEHTGQSYVSSDPEDCDMMQDCVEDRKYPGEVTTPSFKVKFQPKDSKKELLSCPTPGCDGSGHITGNYASHRSLSGCPLADKSLRSLMAAHTPELKCPTPGCDGSGHITGNYASHRSLSGCPRAKKSGIKTPTKDNQEDSELLKFVPQTLQICQATQKQNSQNPTTHPWHNKTCPELPSLPAKIPQHAISSMEKNLKSIEHENKMIEEQNEALFMELSGLSRALIRSLANIRLPHMEPITEQNFDNYISTLTDMYTNKECFQSPENKALLESINKAVKGIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KNY7</accession>
    <name>A0A3B5KNY7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sodium/hydrogen exchanger</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KNY7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001848"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001875.1">
        <property type="protein sequence ID" value="ENSXCOP00000001848.1"/>
        <property type="gene ID" value="ENSXCOG00000001497.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156338"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098719">
        <property type="term" value="P:sodium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004709">
        <property type="entry name" value="NaH_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001970">
        <property type="entry name" value="NHE-1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00840">
        <property type="entry name" value="b_cpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="SODIUM/HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110:SF192">
        <property type="entry name" value="SODIUM_HYDROGEN EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01084">
        <property type="entry name" value="NAHEXCHNGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01085">
        <property type="entry name" value="NAHEXCHNGR1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0050">Antiport</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="10 11" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017270677" description="Sodium/hydrogen exchanger" evidence="14">
        <location>
            <begin position="30"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="137"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="326"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="387"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="425"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="15">
        <location>
            <begin position="85"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="507"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="511"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="563" mass="63513" checksum="F6C2AF5CC9D02A41" modified="2018-12-05" version="1">MAVLGPGSASLRFGSVLLIVLILCGSSWTRDVLTGRNDTRPEPNREHHDQDQDLAANATKHKKAFPVLELNYDYVRMPFEISLWILLALLMKLGFHIIPRVSHIVPESCLLIFVGLLVGGAIKAIQEKAPVMDTKLFFLYLLPPIILDAGYFLPIRAFTENMGTILVFAVVGTLWNAFFIGGMMFAVCQIEAAAAAHVSLLSCLLFGSIISAVDPVAVLAVFEEIHINELLHILVFGESLLNDAVTVVLYHLFEEFSHAETVTVGDAFLGLVSFFVVSLGGILVGVIYGVLGAFTSRFTSHSRVIEPLFVFLYSYMAYLSAEVFHLSGIMSLIACGVVMRPYVEANISHKSYTTIKYFLKMWSSVSETLIFIFLGVSTVEGPHNWNWIFVIFTLIFALLSRVLGVVGLTFIINKFRIVKLTKKDQFIVAYGGLRGAIAFSLGYLIHEDGDSKNTRGMFLTAIITVIFFTVFVQVLLFKRFFSAFPFCPTFRELVFILFASWFSSPAPNQMKSKERRSPGRSSRYDDYERDGWRRRSRSRSYERYRSRSPSYDRRRRRSESPRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KPD4</accession>
    <name>A0A3B5KPD4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphatidylinositol 4-kinase, catalytic, alpha b</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000001983"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002014.1">
        <property type="protein sequence ID" value="ENSXCOP00000001983.1"/>
        <property type="gene ID" value="ENSXCOG00000001552.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074798"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05167">
        <property type="entry name" value="PI4Kc_III_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000005">
        <property type="entry name" value="Phosphatidylinositol 4-kinase, catalytic, alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000009">
        <property type="entry name" value="Phosphatidylinositol 4-kinase, catalytic, alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045495">
        <property type="entry name" value="PI4K_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF15">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19274">
        <property type="entry name" value="PI4K_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="4">
        <location>
            <begin position="1720"/>
            <end position="1968"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1968" mass="222595" checksum="1E5CF4DD8AD89C77" modified="2018-12-05" version="1">MCPVEVRGVFTLDVRRRDAVIALAVFLVESGLQHKDMVVPYLLSLLKGLPRVQWIEEGLGKKEKDFLPVAENFSFCLVTILSDVAQREPQSRLDILSTILEVMGVFQDIIQNPDNHDKVFLCRYIIPSLLGMTRAFGRYSHSEEALLSKLFRKDLPQIRCPTEENESIRRRSFNDFRSIMPSALLSVVQGNNVCRRSACSLDSSVQVGVRFIMPNPTFNLSLVAVPDNSSVEPDYYFTTVSSSFSMSPLFMAACENEVEVPLHLLQQLLRMVKNFASDSLLKTLDTLLTEVTESNAGINLYYKTFSDPLYVCVFKMLRDTLYNLKALEYAFVREVHDFVLEQFSSSQSELQRVLHNTAGLPGEQSLLKLRCQANAACVDLMVWAIKDEQGAENLCNKLSEKLQSKTSSKIVIAHMPLLICCLQGLGRLCERFPVVAHSASNSLKDFLVVPSPVLTKLYKYHSQHSTGDIKINVTNEYCQSTVNIFTNKRDQPSMYEQLRDISIDNICRCLQAGLTLDNVIVEAFLASLSNRLYISQEKEKDAHLIPEHTIKALGHIAVALRDTPKVMEHILQILQQKFCQPPSQLDVLIIDQLGCMVITGNLYIYQEVWNLFQQISVKASSVVYSATKDYRDHGYRHCSLAVINALANIAANLQVEQLVDELLVNLLELFVQLGLEGKRASERASDKGTALKASSSAGNLGVLIPVIAVLTRRLSPIKEAKPRLQKLFRDFWLYSVVMGFAAEGSGLWPEEWYEGVCEIATKSPLLTFPSGEPLRSELQYNSALKNDTVTPAELSELRSTVVNLLDPSPEGAVLINKLDFAMSTYLLSVYRLEYMRMSRANDADRFQVMFRYFEDKAIQKDKSGMLQCVICVGDKVFDVFLQMMAEKTKTKEHEEELERHAQFLLVNFNHTHKRIRRVADKYLSGLAETFPHLLWSGRVLKTMLDILQTLSLSLNADIHKDQPYYDIPDTPHRITVPDTYEARESIVKDFAARCGEILKEAMKWAPSVTKSHLQEYLNKHHIWVSGLSQHTGLAMATESILHFAGYNRQSTILGITQLTERPECVKKDYSNFMASLNLRNRYTGEVVGMIQFSERSHSSSDLTKLMISQMTSTLERKDPEAFTQCMFKMAALCISDCDPQLLHHLCWSPLKMFSEHGMETAIACWEWLLAAHNGLEVPFMREMAGAWQMSVELKMGLFSETQAGAGPLAVSEESQPAPCAPDVVPHFLWIEFLVQRFEIAKYSSADQVEIFISILQRSLSLNIGGSKSNMNRHVAAIGPRFRLLTLGLTLLHADVVTNATIRNMLREKIYSTAFDFFSVAPRFPTQADKRLREDISIMIKFYACLLSDKKYLTATQLVPPDSRNSLDAAIGQRQQITQGWINTYPLTSGMSTISKKSGLSKENNRGSELHKYYMKRRVLLLALLASEIERLTIWYNPLSTQELAIATEQSVETSIANWRSKYISLSEKQGKDNVNMAWTISPYLALQLPARFKNSKSIVSEVTRLVRMDPAAVCDIPEAVKFLVTCHTIDADSPELSHILCWAPVDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPMGYVREYILWAAQKSQLLAHQFIWNMKTNIFLDEEGHQKDPDIGELLEEMVKEIIGSLSGPAKDFYQREFDFFNKITNVSAIIKPIPKGEERKKACLEALAQIKVQYGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCRVSELEKEGLQCHLRSEDETGVEEKASTCWQAAIFKVGDDCRQDMLALQIISLFKNIFQMVGLDLYVFPYRVVATAPGCGVIECIPNCKSRDQLGRQTDFGMYDYFRNQYGDESTLAFQKARYNFIRSMAAYSLLLFLLQIKDRHNGNIMLDSQGHLIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPYMDAVVSLVNLMLDTGLPCFRGQTIKLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQW9</accession>
    <name>A0A3B5KQW9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Procollagen, type V, alpha 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000000867"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000877.1">
        <property type="protein sequence ID" value="ENSXCOP00000000867.1"/>
        <property type="gene ID" value="ENSXCOG00000000698.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159211"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1000:FF:000002">
        <property type="entry name" value="Collagen XI alpha 1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050938">
        <property type="entry name" value="Collagen_Structural_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000885">
        <property type="entry name" value="Fib_collagen_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456:SF6">
        <property type="entry name" value="COLLAGEN ALPHA-1(XXIII) CHAIN-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37456">
        <property type="entry name" value="SI:CH211-266K2.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01410">
        <property type="entry name" value="COLFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00038">
        <property type="entry name" value="COLFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51461">
        <property type="entry name" value="NC1_FIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Fibrillar collagen NC1" evidence="6">
        <location>
            <begin position="1156"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="116"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="57"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="287"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="305"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="446"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="462"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="509"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="538"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="658"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="726"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="801"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="997"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1028"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1072"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1085"/>
            <end position="1103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1384" mass="134230" checksum="42941B61C754E0AF" modified="2018-12-05" version="1">VDGARGEKGQKGEPAVIEPGMLMEGPPGPEGPTGLPGPPGSSGPPGSPGDPGERGPLGRPGLPGADGLPGPPGTVLMLPQFRLSAGGDSGQKGPAVSAQEAQMQAIMQQARLAMRGPTGPMGLTGRPGPLGPPGVPGLKGESGEAGPQGPRGPLGSAGPTGKPGRRGRSGADGARGMPGQTGPKGDRGFDGLAGLPGEKGHRGEPGPSGPPGAPGEDGERGDDGEIGPRGLPGEPGVAGMDGHPGPKGNIQGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPTGEKGHMGPAGPQGPIGYPGPRGGADGVRGLKGTKGEKGEDGFPGFKGDMGIKGDRGELGPAGPRGEDGPEGPKGRSGLPGDAGPLGPTGEKGKLGVPGLPGYPGRQGPKGSQGFQGFPGANGEKGSRGTGGKPGPRGQRGPTGPRGERGPRGPTGKAGPKGNSGNDGPPGPPGERGLPGPQGPTGFPGPKGPPGPPGKDGLPGHPGQRGETQGFQGKTGPPGPPGVVGPQGPTGETGPMGDRGHPGPPGPPGEHGLPGAAGKEGAKGDPGPAGPTGKDGPPGQRGFPGERGLPGPVLQGAHGLKGNEGPQGPPGPAGSPGERGPAGPAGPTGPPGRSGPQGPPGPAGEKGGPGEKGPQGPAGRDGIQGPVGLPGPGGPPGPPGEDGDKGELGEPGQKGSKGDKGEHGPPGPTGPQGPVGAPGPAGADGEPGPRGQQGMFGQKGDEGSRGFPGPPGPVGLQQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGAPGADGPQGPPGGVGNPGAVGEKGDAGETGEPGLQGEVGPPGPRGERGEKGEAGPAGAAGPPGPKGPPGDDGPKGSPGPSGFPGDPGPPGEPGPAGLDGPAGDKGDDGEAGQPGSPGPTGESGPSGPPGKRQGPPGASGPEGRQGEKGAKGEPGIEGPQGKTGPVGPQGSPGKPGSEGLRGIPGAVGEQGLPGSPGPDGPPGPMGPPGLPGLKGDTGVKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPPGTSGPKGDNGIAGPTGPLGPVGPPGLPGPPGPKGAKGSSGQTGPKGETGTPGLPGPPGPPGDVIHPLPIVSPVKGRTRRNIDASQMVDDAAMDANYKDYDDGMEEIFGSLNSLKLEIEQMKHPLGTQSNPARTCKDLQLCHPDFPDEYWIDPNQGCSRDSFKVHCNFTAGGESCIYPDKKSEGSKMAKWPKDSPGTWYSHYKRGSLLSYVDAEGNPIGVVQMTFLRLLSAAARQNMTYNCYHSVAWHDQEQDNYDKAIRFLGSNDEEMSYDNNPYIRAVVDGCALKKNYEKTVLEINTPKVEQVPVVDIMFNDFGGSTQKFGFEVGPVCFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQZ6</accession>
    <name>A0A3B5KQZ6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SRCR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQZ6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000061.1">
        <property type="protein sequence ID" value="ENSXCOP00000000061.1"/>
        <property type="gene ID" value="ENSXCOG00000000048.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157963"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331:SF468">
        <property type="entry name" value="SCAVENGER RECEPTOR CYSTEINE-RICH TYPE 1 PROTEIN M160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19331">
        <property type="entry name" value="SCAVENGER RECEPTOR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SRCR" evidence="5">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="5">
        <location>
            <begin position="112"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="5">
        <location>
            <begin position="203"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="272"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="313" mass="35157" checksum="38C1140E227E5358" modified="2018-12-05" version="1">FSSSVKNENNDNPCEGHVEVYYGNEMGHVGDKNWNEKTDEVVCRSTHCGRPVRKSNVYRAFNSTVWLNELECRGDEASLWDCDGWPGPKVKEDDATRLCNSLPGCKGLEVRLQDGPGKCAGLLEVKDEGQWKRVSKEGWDASYTQRACRLLDCGDAGKDKLDSDEFSQGTAAMLTFSCKEADKNLTDCKTTKTNNQNRNEKALLFLDGPQSCSGYVAIEYKEELYWLSGSNETWNTELANKVCQQMHCGKASSFSPRFSEDKNRMWEESFSCSSDHKSIFDCNKKQINSNNSAIAHVKCEGNEHNPDAIYISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KT01</accession>
    <name>A0A3B5KT01_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Neuritin 1b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the neuritin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KT01"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000746.1">
        <property type="protein sequence ID" value="ENSXCOP00000000736.1"/>
        <property type="gene ID" value="ENSXCOG00000000626.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063853"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990138">
        <property type="term" value="P:neuron projection extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026144">
        <property type="entry name" value="Neuritin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15902:SF1">
        <property type="entry name" value="NEURITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15902">
        <property type="entry name" value="NEURITIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15056">
        <property type="entry name" value="NRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017296424" description="Neuritin 1b" evidence="9">
        <location>
            <begin position="18"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="147" mass="16063" checksum="2331B5975DE29A2B" modified="2018-12-05" version="1">MLVKELLLSSFSSVCCSFSVKLCDYFPVLQTVLVGAGECDSVFKGFSNCLIRLGDKMANYPQDLDDRESLNKICTYWDNFHSCATTALADCQEGAADLWEKLKKESRNLNFRGSLFELCGGGNGAPRSGGLGLLLTALPTVLTWLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTL0</accession>
    <name>A0A3B5KTL0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Hyccin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTL0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001842"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001869.1">
        <property type="protein sequence ID" value="ENSXCOP00000001842.1"/>
        <property type="gene ID" value="ENSXCOG00000001491.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011295"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018619">
        <property type="entry name" value="Hyccin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31220:SF3">
        <property type="entry name" value="HYCCIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31220">
        <property type="entry name" value="HYCCIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09790">
        <property type="entry name" value="Hyccin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="327"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="500"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="349"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="376"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="506"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="522" mass="58197" checksum="1D5392233E4BB96E" modified="2018-12-05" version="1">MLGSERGVVEEWLSEFKSLPETHIPSYAGSLHLKKSLVPALYRVIQDPNNELLEPVCHQLFELYRSSEDRLRRFTLQFLPELVWVYLRITASRDRQSNGCIEALLLGIYNLEIVDKDGNSKLLSFTIPSLSKPSIYHEPSSLGSMALTEGALCQHDLIRVVYSGLHPQRETFTAQNRFEVLCFLMLCYNSAVVYMPLSSYQAVCRMSSRVCVCGFPRQQQKLWTEPCNRVLLDPEFMVQMLTAVYHAIYNGEWEMGREALEDIVYRAQLELYSQPLLLGNAMKNSLPESAPEAPRGRKVLQVEVTPTISRISISAITTASIRRHRWKREDADGMSGGEDSFNINDPDEGFSSGASNSSQPSGTKSGGVGPRGGSLNKREREAEKQAEKCVDQQATPPRPRQRQQSPPGSIKLDAIHLSPIKKHLSFPAGHLLVRTGSTSSSKSFDCMNLNLNGRENELEEAAGGFDKETGPLLGGSHRHSTVSLQEEHLNRPEEAQALLSPGAPLTKQSRSPSFNMQIISQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KU82</accession>
    <name>A0A3B5KU82_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PXA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <authorList>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KU82"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001540"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001565.1">
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        <property type="gene ID" value="ENSXCOG00000001141.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
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    </dbReference>
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        <property type="term" value="F:phosphatidylinositol binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Phox_assoc"/>
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        <property type="entry name" value="SORTING NEXIN"/>
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    </dbReference>
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        <property type="entry name" value="SORTING NEXIN-14"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51207">
        <property type="entry name" value="PXA"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="PXA" evidence="2">
        <location>
            <begin position="237"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="309" mass="34865" checksum="80682EBCC9A0947A" modified="2018-12-05" version="1">MISESAGWPCSNTVIPGLCRTWQPGAGLKPSCPGSRFGLLENCPTRTKHRVKAAGGPEQEPGRVTGCFWTGWVLVNKGTPRFRWKFLSRQSSAEPSSFRTWLLRAAVPGGMASWRRYMEVLVRGVKVLREAGRQYPLSCCLLLVLLGLTVLLHRFLHVFLMFWSLLAGFVTFYFSLGPDSLLPNVFFPVKPRAKVGPDPELFPLGHSCAVCGTTRCQRHRPGLLLENQQPWLDLRVHSKVDASVAEVFELVLEKFVYPWYRDITEDDAWLDEVRTTFRFFSSVLIRRAQKVTGSEPDLLRAWAATPVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KUT0</accession>
    <name>A0A3B5KUT0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSXCOT00000001389.1">
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        <property type="gene ID" value="ENSXCOG00000001118.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009704">
        <property type="entry name" value="EURL_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15961">
        <property type="entry name" value="PROTEIN EURL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15961:SF3">
        <property type="entry name" value="PROTEIN EURL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06937">
        <property type="entry name" value="EURL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="81" mass="9353" checksum="CABA68813420BE35" modified="2018-12-05" version="1">MDEEEQFVNIDLNDDNICSVCKLETETGTLSFCHVCFELSIEGKKRNWRRGSELFDCGEYVTITSFACPRNYCDKESNIVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KV64</accession>
    <name>A0A3B5KV64_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KV64"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001895"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001923.1">
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        <property type="gene ID" value="ENSXCOG00000001537.1"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RNA PROCESSING PROTEIN LD23810P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23140:SF3">
        <property type="entry name" value="SR-RELATED AND CTD-ASSOCIATED FACTOR 4"/>
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        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
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    </dbReference>
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        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CID" evidence="2">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="154" mass="17241" checksum="E5BA387DA5818163" modified="2018-12-05" version="1">MEAVNAFNQELFSLMDSKPPISRAKMISITKSAIKAMKLYKHVVQIVEKFIKKCKPEYKVAGLYVVDSIVRQSRHQFGPDKDVFGPRFTKNITGTFENLCLCPIEDRSKIVRVLNLWQKNGVFKIEVIQPLLDMAAGSGSGAASITHTEEPGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KW55</accession>
    <name>A0A3B5KW55_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KW55"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004348"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004401.1">
        <property type="protein sequence ID" value="ENSXCOP00000004348.1"/>
        <property type="gene ID" value="ENSXCOG00000003432.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000155728"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000027">
        <property type="entry name" value="pleckstrin homology domain-containing family A member 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12752">
        <property type="entry name" value="PHOSPHOINOSITOL 3-PHOSPHATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12752:SF3">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY A MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="WW" evidence="5">
        <location>
            <begin position="13"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="173" mass="19503" checksum="2984E536E8D9BD11" modified="2018-12-05" version="1">MAADPKPDWLCCLPSSWSYGVTRDGRIFFINEEAKSTTWLHPVTGEAVITGHRKTPDLPTGWEEGYTFEGARCFIKLSSSTMPPLYSMPEQEHGTGVTGDRKGNQGWALLLIKSCVLVLYSAGSHHINDPTKRNNPVKCIMMRNPAQLIEVSFSHFTVVYNSMRLFHWGYSTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KX47</accession>
    <name>A0A3B5KX47_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027894431.1">
        <property type="nucleotide sequence ID" value="XM_028038630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KX47"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003112"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003149.1">
        <property type="protein sequence ID" value="ENSXCOP00000003112.1"/>
        <property type="gene ID" value="ENSXCOG00000002457.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114157558"/>
    <dbReference type="CTD" id="256471"/>
    <dbReference type="GeneTree" id="ENSGT00530000063854"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17326">
        <property type="entry name" value="MFS_MFSD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051068">
        <property type="entry name" value="MFS_Domain-Containing_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23510">
        <property type="entry name" value="INNER MEMBRANE TRANSPORT PROTEIN YAJR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23510:SF3">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="466"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="33"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="501" mass="55202" checksum="DC961B31E3310693" modified="2018-12-05" version="1">MSHLDPDDTTPLLRDDASSDDSQDEDYRGRWRSIRVMYFTMFLSSVGFTIVITSLWPYLQKIDGSADASFLGWMVAAYSLGQMVASPLFGLWSNHRPRREPLVCSIFINLAANIYYAYACLPKTNNKYHMLMSRAFVGFGAGNVAVVRSYVAGATSLNERTGAMANMSACQALGFILGPALQACLSFIGENGVTVKFLDLQLNMYTTPALLAAVFGLINILLVLLVLKEHRVNEDGKHIRSINYISEVDILDESVEAIDQVAVVTSNVLFFIVMFIFAVFETIATPLSMDMFAWTRKEAVLYNGIIMVGIGFQSILVFLLVKAAATRFGDRPVLLAGFGIIFCGFIILLPWGNHFPKIQWADLKNNSLVSQMSEGNGTVEPTGCPYEQTWCQTTPAIYLAQYISSDFLIGVGYPACNVMSYTLYSKILGPKPQGVYMGWLTASGSGARTLGPVFVSHVYTLLGPRWAFGLICGMVVLGVILLLSAYHRLIAFSVRHGRIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXB1</accession>
    <name>A0A3B5KXB1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXB1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003187"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003224.1">
        <property type="protein sequence ID" value="ENSXCOP00000003187.1"/>
        <property type="gene ID" value="ENSXCOG00000002509.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159641"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004846">
        <property type="entry name" value="PRK06197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF59">
        <property type="entry name" value="RETINOL DEHYDROGENASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="326" mass="35393" checksum="DE85B431A990FA1E" modified="2018-12-05" version="1">MSRYILPVSVFGTVNNATGTAGIKGKTVVITGANTGIGKETARELANRGGRIIMGCRDMEKCEAAAKEIRGKTLNPHVYACQLDLASMKSIREFAERINQEEKSVDILINNAGVMRCPAGKTEDGFDMQLGVNHLGHFLLTNLLLEKLKDSAPSRVINLASLAHIVGKIDFDDLNWERKKFDTKQAYCQSKLANVLFTRELAKRLEGTGVTVNAVHPGVVATDLGRHTGLHQSQFSTTVLSPFFSMLVKSPELGAHPSVFLAVAEEMAGVTGRYYDVMTEKEPAPQALDDAAARRLWEVSSRLVGLRDDTPPQTTTGLTEPEPAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXY7</accession>
    <name>A0A3B5KXY7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLITRK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXY7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000419"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000427.1">
        <property type="protein sequence ID" value="ENSXCOP00000000419.1"/>
        <property type="gene ID" value="ENSXCOG00000000369.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161248"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051965">
        <property type="term" value="P:positive regulation of synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000001">
        <property type="entry name" value="SLIT and NTRK-like family, member 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45773:SF4">
        <property type="entry name" value="SLIT AND NTRK-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45773">
        <property type="entry name" value="SLIT AND NTRK-LIKE PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017458152" evidence="11">
        <location>
            <begin position="24"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="605"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="12">
        <location>
            <begin position="32"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="13">
        <location>
            <begin position="220"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="12">
        <location>
            <begin position="341"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="13">
        <location>
            <begin position="531"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="272"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="857" mass="96434" checksum="14F4F18C4F86827E" modified="2018-12-05" version="1">MLSGVLFLSVLTVTSLTPSETQSRKTSALKDICKTRCDCEERENVLNINCENKGFTTVSQLQAPPNKVSQLFLNANFLSRLSANEFVSYGNVTSLHLGINGLQEIQTGAFNGLRFLKRLHLNNNKLEVIKEDTFAGLESLEYLQADYNYISTIEPGAFSKLNKLKVLILNDNLLLALPPNIFRFVLLTHLDLRGNRLKMLPFAGVLEHIGGIMEIQLEENPWNCTCDLIPLKSWLDTISVFVGDIVCETPFRLHGQDITQLIKQDLCPRRNAGERAQSASDSHFQGAPHPTYHAGVVTPTRSPKASRPPKMRYRPTPRLSKDKHVFGPIMVYQTRSPVPMLCPSVCVCTSQNPDSGLNINCQERKLQNISELNPKPTYPRKLHLTGNYLQMIYRTDLTEYSSLELLHLGNNRIAAIQEGAFENLTNLRRLYLNGNYIESLSQSLFAGLQSLQYLYLEYNIIKEILPQTFNSLHNLQLLFLNNNLLRSLPDNVFGGTMLTRLNLRNNHFSYLPVQGVLDQLSAFIQIDLQENPWDCTCNIVVLKNWMELSSTSVVVNEITCDSPSKHAGRLLRSLRNEAICPEPSDSVSPTDSHLQTPELHPEVPLSVLILGLLVVFILSVCFGAGLFVFVLKRRKGVEHVPTGANNLDLNSFQVQYGSYTPEPTHDKSSESHVYNYIPPPVGSMCQNPIYMQKDGEQVAYYRNLKELSFRPLDAKKEDILTRSPGTYTISTVDFMDKSPTCGLSSPEPPEMLYQNIGERPNKELPTAAGAPPFTYNFCTLPKRPCIVPPYEAAAARRHLTNPDRLNKAVLYGTPRKYFGPEHFSKNNEHPSLLLPGKLKTEPDYLEVLEKQTAMSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KY03</accession>
    <name>A0A3B5KY03_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WD repeat domain 20a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KY03"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004983"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005043.1">
        <property type="protein sequence ID" value="ENSXCOP00000004983.1"/>
        <property type="gene ID" value="ENSXCOG00000003910.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007686"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051362">
        <property type="entry name" value="WD_repeat_creC_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14107">
        <property type="entry name" value="WD REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14107:SF5">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="17725" checksum="83AAC15A365AB064" modified="2018-12-05" version="1">MAAEGGGKEMNEIKTQFTTREGVYKLLTHSEYSRPNRVPFNSQGSNPVKVSFVNVNDQSGNGDRICFNVGRELYFYIYKGVRKAADLSKPIDKRIYKGTQPTCHDFNHLTATAESVSLLVGFSAGQVQLIDPIKKETSKLFNEEGLLSSQNQASSPSGTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYM1</accession>
    <name>A0A3B5KYM1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KYM1"/>
    <dbReference type="SMR" id="A0A3B5KYM1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000649"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000659.1">
        <property type="protein sequence ID" value="ENSXCOP00000000649.1"/>
        <property type="gene ID" value="ENSXCOG00000000567.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158086"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12683">
        <property type="entry name" value="RRM_RBPMS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000037">
        <property type="entry name" value="RNA-binding protein with multiple splicing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034787">
        <property type="entry name" value="RBPMS2_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10501">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A/U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="16"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="195" mass="21222" checksum="1763CBE926514A0E" modified="2018-12-05" version="1">MIPCTPEPNSDSSSVRTLFVSGLPVDIKPRELYLLFRPFKGYEGSLIKLTSKQPVGFVTFDSRSGAEAAKNALNGIRFDPESPQTLRLEFAKANTKMAKSKLMATPNPTNIHPALGAHFIARDPYDLTGAALIPASPDAWTPYPLYTTELTPGLPHAAFTYPAAAAAAAALHAQMRWYPTPSETSQPGWKSRQFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYS4</accession>
    <name>A0A3B5KYS4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="29">Cadherin, EGF LAG seven-pass G-type receptor 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Receptor that may have an important role in cell/cell signaling during nervous system formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000003707"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003747.1">
        <property type="protein sequence ID" value="ENSXCOP00000003707.1"/>
        <property type="gene ID" value="ENSXCOG00000002895.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160077"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000011">
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032471">
        <property type="entry name" value="AGRL2-4_GAIN_subdom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056286">
        <property type="entry name" value="Cadherin_CELSR1-3_9th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
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    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
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    <dbReference type="PANTHER" id="PTHR24026:SF38">
        <property type="entry name" value="CADHERIN EGF LAG SEVEN-PASS G-TYPE RECEPTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026">
        <property type="entry name" value="FAT ATYPICAL CADHERIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23592">
        <property type="entry name" value="Cadherin_CELSR2_9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="EGF_laminin"/>
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        <property type="entry name" value="GAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
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    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="8"/>
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        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 21" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="15 22" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 22" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="19" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="20 23" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="18" id="KW-0807">Transducer</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="28">
        <location>
            <begin position="24"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="28">
        <location>
            <begin position="132"/>
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        <location>
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        <location>
            <begin position="348"/>
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    <feature type="domain" description="Cadherin" evidence="28">
        <location>
            <begin position="453"/>
            <end position="554"/>
        </location>
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    <feature type="domain" description="Cadherin" evidence="28">
        <location>
            <begin position="555"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="28">
        <location>
            <begin position="658"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="28">
        <location>
            <begin position="764"/>
            <end position="865"/>
        </location>
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    <feature type="domain" description="EGF-like" evidence="25">
        <location>
            <begin position="1064"/>
            <end position="1122"/>
        </location>
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    <feature type="domain" description="EGF-like" evidence="25">
        <location>
            <begin position="1124"/>
            <end position="1159"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="25">
        <location>
            <begin position="1163"/>
            <end position="1201"/>
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    <feature type="domain" description="Laminin G" evidence="24">
        <location>
            <begin position="1202"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="25">
        <location>
            <begin position="1405"/>
            <end position="1441"/>
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        <location>
            <begin position="1447"/>
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            <begin position="1663"/>
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    <feature type="domain" description="Laminin EGF-like" evidence="26">
        <location>
            <begin position="1757"/>
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    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="27">
        <location>
            <begin position="1789"/>
            <end position="1859"/>
        </location>
    </feature>
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        <location>
            <begin position="1112"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="1431"/>
            <end position="1440"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="1691"/>
            <end position="1700"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="1757"/>
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    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="1759"/>
            <end position="1776"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="1778"/>
            <end position="1787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010933"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
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            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
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    <evidence type="ECO:0000259" key="24">
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    </evidence>
    <evidence type="ECO:0000259" key="25">
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    <evidence type="ECO:0000259" key="26">
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    <evidence type="ECO:0000259" key="27">
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    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
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    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="2046" mass="226596" checksum="59E10965A1411EA8" modified="2018-12-05" version="1">EKARRILMPWSRSRRAANRHPHFSLYNYQVQVAENQPPSTSVIIMSATDPDGGDAGRVSYTMAPLVNSRSSDYFHIHPDTGLITSTQILDREHMDLHYFRVTATDYGSPRLSGTTMLTITVSDRNDHSPIFEQSEYRETIRENVEEGYPILQLRATDSDSPSNANIRYRFIGDSAAIARAAFEIDPRSGLITTRGSVDRETNEHYTLQVEASDQGKDPGPRSATVKVYITVLDENDNVPQFSEKRYVVAVKEDVRPHSEILRVSATDRDKDSNAAVHYNIISGNSRGQFSIDSVTGEIQVVAPLDYESEREYTLRVRAQDNGRPPLSNNTGIVSVQVTDVNDNPPIFVSTPFQASVLESAPVGSSILHIQAIDTDSADNARLEYRLTGTGSDTPFVINSATGWVTVSSILDRESVEHYFFGVEARDYGMPPLSATASVTITVMDVNDNRPEFLQKEYYSRLNEDAAVGTSVVTVTAVDRDVNSAVTYQITGGNTRNRFAISTAGGAGLLSLALPLDYKQERRYVLTVTASDRTLHDTCQVHINITDANTHRPVFQSAHYSVSVNEDRPPGSTVVVISATDDDVGENARITYFLEDNIPQFRIDPATGAITLQAELDYEDQMTYTLAITAKDNGIPQKSDTTYVEVNVNDVNDNAPQFLSPRYQGTVSEDAPPFTSVLQVSATDRDAHANGRVQYTFQNGEDGDGDFTIEPTSGIVRTVRRLDRESVPFYELTAYAVDRGVPPQRTPVHIQVTVLDVNDNAPVFPADDFEVLVKENSAVGSVVAQITATDPDEGANAQIMYQIVEGNIPEIFQMDIFSGELTSLIDLDYEARNEYVIVVQATSAPLVSRATIRIRLVDQNDNRPTLQDFQIIFNNFVSNRSNSFPSGVIGRVPAHDPDVSDRLYYTIDRGNELHLLLLNHTSGEIRLSRKLDNNRPLVAPMLITVTGVHSISAQCVLRVLIITEDMLGSSVTVRLQNMSQEHFLSPLLGNFLEGVSAVLSVPVEDVFIFNIQPDSDATPGRILNVSFSAALPGGHFFPSETLEEQLYLNRPRLTSLTQMEVLPFDDNVCLREPCQNYMKCISVLRFNSSAPFISSPSILFRPIHPIAGLRCRCPVGFTGDYCETEINLCYSNPCLNGGVCARREGGYTCICREDYTDRCELDRRQGRCVSGVCRNGGTCRELSGGGFRCECPPGGYERPYCTVTARSFPPKSFAMFRGLRQRFHLSISLFATLEKSGLLFYNGRFNEKHDFIALEIQEGQVVFKYSTESSTQVSPFLLGGVSDGNWHTVHIHYYNKPRRSLIGEAQGPSDEKVAVVSIDDCDTALSLRFGTQLGNYSCAAQGKQTSSKSLDLTGPLFLGGVPNVPDNFPFGSREFIGCMKDLHIDSKALDLAGFIANNGTLPCSAKHPFCKSNPCQNGGTCRVSWETFSCDCPLGYGGKDCSYAMPHPHRFLGNSALWWDLKNDVTISTPWYLGLVFRTRAREGTLLQAQAGQYTSLLFQVVNGQLVFSVTRGATRPVRLRLDQVQVADGRWHDLQLELRDVRSGRETRYVATLRLDFGLYQGTVIVGNDIHGLKVKHLHVGGVLGSGEVQNGIKGCIQLQGVRLGVRPDAPALPRPSRVVKVETGCNVGNPCVSSPCPAHSRCTDVWERHTCICEPYYGKSCTDACHLNPCENEAQCHRKPSSSHGYICDCGDNHYGQYCQHIDHQCPRGWWGSPTCGPCHCDMNKGFDPDCNKTSGHCHCKEFHYRPRASDTCLPCDCYPVGSFSRSCDPETGQCQCRPGVIGRQCNMCDNPFAEVTNSGVIYDGCPKTITQGIWWPRTKFNLPAAVPCPKGSVAAIRHCDVERGWLEPDLYNCTSPPFVELNIALDSLERNETELNTIMEKKLAHQLRDVTEATGRLYGNDLQIAERLLSRLLTFETQQSGFGLTATQDAHFNENVLRGCSVLLGPATSGLWRALAQNQNHLLGGGPAELTELLEQYSKNIAQNMKLTYLNPVALVSPNIVMNLDRVENHTHVRRRFPRYQNTLFRGQALWDPYTHVILPPAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L079</accession>
    <name>A0A3B5L079_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">WD repeat domain 90</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000003281"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003319.1">
        <property type="protein sequence ID" value="ENSXCOP00000003281.1"/>
        <property type="gene ID" value="ENSXCOG00000002569.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160173"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001066">
        <property type="entry name" value="WD repeat domain 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055440">
        <property type="entry name" value="Beta-prop_WDR90_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055441">
        <property type="entry name" value="Beta-prop_WDR90_POC16_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007714">
        <property type="entry name" value="CFA20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050630">
        <property type="entry name" value="WD_repeat_EMAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720:SF33">
        <property type="entry name" value="HELP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720">
        <property type="entry name" value="WD-40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23393">
        <property type="entry name" value="Beta-prop_WDR90_POC16_2nd"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05018">
        <property type="entry name" value="CFA20_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23342">
        <property type="entry name" value="WDR90_beta-prop_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CFA20" evidence="4">
        <location>
            <begin position="2"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="476"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="WDR90/POC16 second beta-propeller" evidence="6">
        <location>
            <begin position="487"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="WDR90/POC16 second beta-propeller" evidence="6">
        <location>
            <begin position="576"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="868"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1131"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="domain" description="WDR90 4th beta-propeller" evidence="5">
        <location>
            <begin position="1137"/>
            <end position="1442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1445" mass="158311" checksum="3E506A80E30F95C6" modified="2018-12-05" version="1">GWQHPYVNIFKHVKVEEWKRSAKAGDVSSSMDRTLRCPVFSIKGPVPTNSYILVPKSSNQSLGLTGRYFYLLFRPTPGKYFMVHLDVAVEEGQVVRISFSNMFKEFKSTATWLQFPFLCGAQPRTPACLVGPAPDSVRWTCLMMDLCFTLTFYLNRHHAHLKSVKLCANMAVKNMFTSDLLLDPGESQTTKGTGPIPREMSFPVPKGGSWNDFYDHIKFPSDGTKLPFDSIQKPDPALLPDPILRLSRIIGFGGATTRDALWTKSGEEVVYPCHAIIVSMKILSRKQRFFTGHTDKVFYFSQAPNQMVSCGRENVRLWRVRNGSLRSCPVYLGEFHSLDFTDVAFQEGGASDQFASTRSGHILEINYSRVTVKNARRLMPAQQQHAERREKWTFNTGPGIPINSISVSSSFCATGTVDGFLRLWTLDFSAVILEAEHEGPVTVVSVSFDSLQVLSATFTGNLGYLDVSSRGYNTLMRSHTGDVLGFSVDGIRRHLTTASADGTVRVWNTDSLQQLYDFVSMDKPCSVAFHPHEPTFSCGFGSGTVRVFDISSARLLAEHKYAAFPRLLTAAGSDRRRHGNVVARGTERAPDALAVSGDSSRLAFVGPTPHIVTVADARSLDELLHVDVSVLDVESPELDSALKVRFSPASAEHLLVATSANKILWISTKTGRLLRQVSNVHKRPCSSLAVSEDRRFLLTSGHNTVKVWDYKRTNQVNPQVMYIGHSQPIRQVSFTPDQLGVVSVGDAIFLWDFLAEPNECLIGNGKVNAAQLSNGMPRQAAPLPSSQHQLDVSAMDPEIQGGMLVLIIRLVKTVPRPQDECIKLKAVIGYNGNGRGNMGWSPDQGLFAYSCGGVVVLEHLHTGTQSHLQGHSEEISCLAISNDAQTLVSAAGSSRSLVSVWDVRTAACRGTASHHKGAVQSLAFSRDDLFFLSVGDFFDPVLALWSSESVQLLCSVSMSAPIHDAAFSPAAARQLACVGSEGIHFCLIHTRAANTDIKACRVSAPSEVGDVELTALCYHMDSFLLTSTTGGHVCAWDVNKQRCFMVWRADEGEIGVLLCRENRLLTGSATGRLQLCVTLGGSKVVVEQEVVLDGKLVSAAFDNSMEMGIVGTTAGTLWYINWSDSSIIRLVSGHRAKVNDVVFSRDESRFATCSQDGSVRVWSTGSFELVVQFQVINQACHCLCWSPSSSSDGSRLAAGYGDGTLRIFQLASTEMEMKLRPHQEAISAITYSASGEKTLPGSKGLVAVSSPISGATIRVIRDHKGAAINSIQSVSEQCKRFGLEGNEMFLVAGADRRVSVWAANWSKEKCDLLDWLSFPAPPSSEVRLLLPPSLAAFYPADPSLLLYTGYGLKKELSFYSLTKKQIIKKISLTHWATCISLSPRGHLLAVGCKERVLKLIKSTSGRFQDFLLHSDALQTCRFSPSGSLLFSVAYDEVLLWEVPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L0V2</accession>
    <name>A0A3B5L0V2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L0V2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004485.1">
        <property type="protein sequence ID" value="ENSXCOP00000004432.1"/>
        <property type="gene ID" value="ENSXCOG00000003499.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154446"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003017">
        <property type="entry name" value="Si:cabz01054396.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000512">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002239">
        <property type="entry name" value="Zinc finger protein 226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="263"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="291"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="319"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="347"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="381" mass="44418" checksum="27A0707B9DBDE15E" modified="2018-12-05" version="1">MSQTAGSRNKTQETVPELLVIKEEGPFEWSSSEDEQDPELFHIKEENEESWTNQEGQQFYGEEDLASFPLNVIVKSEEEEEYQSSQLYLRETEHNRESKPLCSVHFIKTEPDEGPESTIKPGLSNYFKIKTAGKASDSSETEVSDEDNHWQEPLPDFGAEIKHKESRTPDSGNTAKALYSCFECDKQYFYKQSLHRHMKCHSSKRVFICDVCGHEFNQRTNLTTHKRVHTGEKPFRCDVCGKRFNQRGNLRNHKRIHTGERPFICDFCGERFRNQGILQAHMRVHTGEKPFVCGVCGRKFSRKTHFETHMRVHTGERPFSCEICGKDFRHKMHYQEHIRVHTGELPFPCEVCGKKFRLQCNLKRHLRVHTGEKPFMEGEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1A1</accession>
    <name>A0A3B5L1A1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1A1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001604.1">
        <property type="protein sequence ID" value="ENSXCOP00000001579.1"/>
        <property type="gene ID" value="ENSXCOG00000001277.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000271568"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="79" mass="9273" checksum="2DCE5039EBC5CC10" modified="2018-12-05" version="1">RHSVRRHINIPLSSDKQHWKILESSFGDETSHLPPCCCWYAPQIQAPYIFHTQKEFHMLYTLFLNEVCSLMIPTGCIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2Y9</accession>
    <name>A0A3B5L2Y9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Collagenase 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Matrix metalloproteinase-13</fullName>
        </alternativeName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="21">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="21">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2Y9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002149"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002180.1">
        <property type="protein sequence ID" value="ENSXCOP00000002149.1"/>
        <property type="gene ID" value="ENSXCOG00000001716.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166189"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00094">
        <property type="entry name" value="HX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000007">
        <property type="entry name" value="Collagenase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.110.10.10:FF:000002">
        <property type="entry name" value="Matrix metallopeptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.110.10.10">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000585">
        <property type="entry name" value="Hemopexin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036375">
        <property type="entry name" value="Hemopexin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018487">
        <property type="entry name" value="Hemopexin-like_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018486">
        <property type="entry name" value="Hemopexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021158">
        <property type="entry name" value="Pept_M10A_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF165">
        <property type="entry name" value="COLLAGENASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00045">
        <property type="entry name" value="Hemopexin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001191">
        <property type="entry name" value="Peptidase_M10A_matrix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00120">
        <property type="entry name" value="HX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50923">
        <property type="entry name" value="Hemopexin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00546">
        <property type="entry name" value="CYSTEINE_SWITCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00024">
        <property type="entry name" value="HEMOPEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51642">
        <property type="entry name" value="HEMOPEXIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 21" id="KW-0106">Calcium</keyword>
    <keyword evidence="14" id="KW-0177">Collagen degradation</keyword>
    <keyword evidence="16 22" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 27" id="KW-0732">Signal</keyword>
    <keyword evidence="11 20" id="KW-0862">Zinc</keyword>
    <keyword evidence="15" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="27">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017396621" description="Collagenase 3" evidence="27">
        <location>
            <begin position="19"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="28">
        <location>
            <begin position="101"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="25">
        <location>
            <begin position="272"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="25">
        <location>
            <begin position="322"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="Hemopexin" evidence="25">
        <location>
            <begin position="370"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cysteine switch" evidence="24">
        <location>
            <begin position="86"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="21">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="376"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphotyrosine; by PKDCC" evidence="23">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="275"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR001191-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR621190-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="463" mass="53052" checksum="E05725067235DB11" modified="2018-12-05" version="1">MFPLLMLLLPVLAQYSFALPLPTGKKENLVTEKKYLRHYYGLPTGLQSRQSSLSDFKNKIQQMQKFFKLQVTGNLDDNTLEVMKLPRCGVPDVGEYNHFPRHLKWENNNVTFRIVNYTPDLKRSDVDRAIRNALNVWADVTPLTFKKMHDGNADIMISFGTKEHGDYNPFDGPDGLLAHAYPPGPGIGGDTHFDEDEHWTKDSSAYNLFIVAAHELGHALGMSHSSDAGALMFPVYSFTSGFPLAEDDIEGIQALYGPNPNPRKRKPKPDAPNKCDPELSFDAVTELRGETIIFKDRFYWRLHPQMAEPEQTLIKSTWPSLPNKVDAAYEYPEKDLVFIFSGIRMWALNGYSVVDGYPKYIHKLGLPKSVRKIDAAVHIKNTGKTLLFADEEYWSYDEATGTMDPGFPKSVEDDFPGMHDEFDAVTFQQGYLYFFHENMQYEYSYRFRKVIRIMRTNSFLNCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L365</accession>
    <name>A0A3B5L365_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L365"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006841.1">
        <property type="protein sequence ID" value="ENSXCOP00000006758.1"/>
        <property type="gene ID" value="ENSXCOG00000005229.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00400000023815"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="174" mass="19544" checksum="6C073D7BFFF69E91" modified="2018-12-05" version="1">AAGVFSLHTVINLSSTMNLRSDQDIPMSVALPIELQADGSHQAFPFLDTTLADLGIQESDVKERVVWVDTKKTQVKNKTGKLKEKETTILEVRVKAKKPGDKQLQEVLYSTEAHTDRSFCRTEMDIQPWKQGDPGLYAAHLCRHLDFTSYKEREELNPACSRTHTATQIAESSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4E0</accession>
    <name>A0A3B5L4E0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">formate--tetrahydrofolate ligase</fullName>
            <ecNumber evidence="2">6.3.4.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
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    <dbReference type="EC" id="6.3.4.3" evidence="2"/>
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    <dbReference type="STRING" id="32473.ENSXCOP00000005682"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005748.1">
        <property type="protein sequence ID" value="ENSXCOP00000005682.1"/>
        <property type="gene ID" value="ENSXCOG00000004422.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:formate-tetrahydrofolate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="entry name" value="Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Putative formate--tetrahydrofolate ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1510.10">
        <property type="entry name" value="Domain 2, N(10)-formyltetrahydrofolate synthetase"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.10.410.10">
        <property type="entry name" value="Formyltetrahydrofolate synthetase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000559">
        <property type="entry name" value="Formate_THF_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020628">
        <property type="entry name" value="Formate_THF_ligase_CS"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01268">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00722">
        <property type="entry name" value="FTHFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="367" mass="39251" checksum="AAD6584D1FE5DFF1" modified="2018-12-05" version="1">MAILALADSLKDMKSRLARMVVGSSRSGEPVTAEDLGVSGALAVLMKDAIKPTLMQTLEGTPVFVHAGPFANIAHGSSSVLADKLALKLVGRDGYVVTEAGFGADIGMEKFFNIKCRASGLRPNVMVLVATVRALKMHGGGPNVSPGAPLPKEYIDENLSLVAGGCHSNLKKQIQNSHLFGVPVVVALNVFKTDTQAEIDLVCQLAKECGAFDAVPCRHWSQGGRGALELAMAVKEAAGRPTNFQFLYNSEMPILEKIRTIAQKVYGAEDIDLSPEASSKIDYYNQQGYESLPICMAKTHLSLSHMPDKKGAPTGFVLPIRDVRASIGAGFIFPLVGTMSTMPGLPTRPCFYDIDLDPETEEITGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5C9</accession>
    <name>A0A3B5L5C9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5C9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007518"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007614.1">
        <property type="protein sequence ID" value="ENSXCOP00000007518.1"/>
        <property type="gene ID" value="ENSXCOG00000005799.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163251"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="206"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="337"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="102"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="544" mass="60904" checksum="3C1C2E8928A110B8" modified="2018-12-05" version="1">MPPDSSAANKVTDYEAATSFLGEWGAFQRRVFFLLSLSVVPNGLTAFSIVFLADTPDHRCALPAHLNLSAAWRNSSIPLEEDAIRDGALVPSKCSRYKVENLLKYSERGLLPLDVNLSNVPKESCLDGWEFDHSVYTSTIVSEWNLVCDESWKSPLTSSIFFCGVFTGSLISGQLSDRWFGRKIVMFATIALQAVSRFIQVFSPSWMVFCALYFIMGMGQVSNYIVAFVLGMEVLGPRTRTVFSTAAVSLFFGVGYMLLPLFAFFITDWRMLLLALMFIPESPRWLLSQGRTEEAEAILRNAAEMNNIEPPTVIFSSLQTEERRPCNICDLLRSPNIRWISITLNTITIAYFALSLNTANLNGNAYFNCFLSALMEMPAYILSWVMFRWCSRRMSVFSSLSSGGLFLLIIQLVPAPALSITFEMLGKFAVSTAFAVVYAYTAELYPTVLRNTAVGACSMASRIGSIIAPFFIYLRNLNMCLTTINFLGVKLQTFLNMKKIKPTFTSDLFWQPDSISLRLKAYCIFSSGSQHVTHGSLVFLFRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5V0</accession>
    <name>A0A3B5L5V0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GMP phosphodiesterase delta subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PDE6D/unc-119 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5V0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007688"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007785.1">
        <property type="protein sequence ID" value="ENSXCOP00000007688.1"/>
        <property type="gene ID" value="ENSXCOG00000005911.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000263"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.40">
        <property type="entry name" value="GMP phosphodiesterase, delta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008015">
        <property type="entry name" value="PDED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037036">
        <property type="entry name" value="PDED_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12976">
        <property type="entry name" value="RETINAL ROD RHODOPSIN-SENSITIVE CGMP 3',5'-CYCLIC PHOSPHODIESTERASE DELTA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12976:SF0">
        <property type="entry name" value="RETINAL ROD RHODOPSIN-SENSITIVE CGMP 3',5'-CYCLIC PHOSPHODIESTERASE SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05351">
        <property type="entry name" value="GMP_PDE_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMP phosphodiesterase delta subunit" evidence="2">
        <location>
            <begin position="54"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="117" mass="12811" checksum="06F5F48FD8EBA028" modified="2018-12-05" version="1">MKQPAGRVCVLEGGPQGLAAGSDVPGGPWGHHGIHITTCYVAGMMELFGVFSLCAEWFFEFGFVIPNSTNTWQSLIEAAPESQMMPANVLTGNVIIETKFYDNDLHVSTSRVRLFYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7K7</accession>
    <name>A0A3B5L7K7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Solute carrier family 26 member 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L7K7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003774"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003814.1">
        <property type="protein sequence ID" value="ENSXCOP00000003774.1"/>
        <property type="gene ID" value="ENSXCOG00000002933.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286960"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018045">
        <property type="entry name" value="S04_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01130">
        <property type="entry name" value="SLC26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="470"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="6">
        <location>
            <begin position="522"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="717" mass="78885" checksum="44D3623E1ED88030" modified="2018-12-05" version="1">MEERRRATYKVNRDVLDEGDLDEMGEKSDTKPSLCDKIKDSVSCTGPRVKNCMLGTFPVAKWLPRYAFREDALGDLVSGISVGIMQLPQGMAYALLASVPPVFGLYSSFYPVLIYFIFGTSKHISIGTYAVMSVMIGGVVDRLAPDSNFEIWDNVTNSSIIDSVSRDQERVRIAVVVTFVSGLFQILLGLVQFGFVVTYLSEPLVRGYTTGAAVHVIVSQLKYVFGIHPKRYSGPISLIYTVLEICYLLPQTNIGTLVVSIVSIVTLILAKELNAYLSKKLPVPLPMELFVIIATVISWQVNLEKRYGVDVVGDIPSGLQPPALPAMSLFGQVVGDAFALAVVGYGIAISLGRIFALKYGYKVDSNQELIALGLSNSIGGIFQCFAISCSMSRSLVQESTGGKTQVAGAVSAVVILFITLWIGSLFEDLPKAVLAAIIFVNLHGMMKQFLDIPALWKSNKIDMLVWVATFILTVLLNPDLGLAAAIAFSMLTVIFRTQLPKYSLLGQVADTDIYKPVEDYDKQVPGILIFRSSATLYFANAEMYQEALGEKSGMDITKILSIKKKLAAKRKRHEKKLAKKVKKELKKNKEPNSEHQKGIAIIDVDNDPDLSLPRAIILDLSPVNFLDTVGVKTIKSIRRDYGEIDIEVILACCQSGVVESLQAGGFFSSNVTKSCLFYSIHDAVLYCQSAKTKNQYDEEYEQEVRAGIHIDRHCVHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7M5</accession>
    <name>A0A3B5L7M5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Synaptopodin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SYNPO2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the myozenin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the synaptopodin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L7M5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006910.1">
        <property type="protein sequence ID" value="ENSXCOP00000006827.1"/>
        <property type="gene ID" value="ENSXCOG00000005096.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183054"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032233">
        <property type="term" value="P:positive regulation of actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008438">
        <property type="entry name" value="MYOZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051976">
        <property type="entry name" value="Synaptopodin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24217">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24217:SF9">
        <property type="entry name" value="SYNAPTOPODIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05556">
        <property type="entry name" value="Calsarcin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="111"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="163"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="510"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="587"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="709"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="804"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="886"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="137"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="217"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="236"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="591"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="606"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="719"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="744"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="822"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="832"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="853"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="893"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="906"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="916"/>
            <end position="928"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1073" mass="118469" checksum="DB1EFBE448B8EFB2" modified="2018-12-05" version="1">MKEKHIGHSKPAQEMEPQAEDPAYGPLWSGSEGSQELCPSERLAEFYNEDDDSESEDHSGSNTHIPSSQSPECLQLQHRESADGPESLLEQSEQKESVFYQENCDFYFSESRESMTESPYIEASQTSVTSPPECPDSKSWQHSCSSSSSLGCAADMTKVLTLSSERPLGASDGHGPSTENRSVESSEEGGSNETPPASVFFGISDEGAEQTETRNLGSDRDLCRPDRQRARYTRLSHKESQSERHVKETKSKCKRIAQLLANAPNPQNKGALLFKKRRQRVKKYTLVSYGTGVHAFDSSGQIEGETDKVRSAGYSFLSSSDSDFEEQYFQRHQYHNPALDWGSGEDMDVLPETKGKGLLMFEQRRKRIDELVSEQEDERSEGFSEDVLKETQRARIYESKEMYMHAEETNVKKHMDYHADVQEVNHLSTVPKPMVSNRTAKPFLGFQASSTLEPVTMSGGVSPAMKKHELAFKVHVPINSNLKVWSPTGDIIASRDERISVPAIKTGILPESKRKTANKHPSVPAKESELYSQSKGERKSYIESEEDFFSLGAEACNFMQPKPIKLKNPPPVAPKPTINPACPPWNPSSEPCVPSRSPVSHPSHKASLQTPANARVPVNSSPKQAHNPTYSPLSHSKNKSDSSPNLVAPFLPQAEKSYLQAPKPLEVFPKLRVPDRSVKQAADGTTMSGKGAKLFAKRQSRMEKFVIDGETVQANKTRSPSPSSSLPNSWRYSSNIRAPPPLSYNPLLSPFYPPSAAKQPPSTSPKPKPKSKEKPKSSPKQLNALDIMKHQPYQLDSSLFTYDSASEAKSPSPKPTPVSKFEVTKSLKQRADSSYSPYNPSEPAGQTKQAQGSRPASTTSQHSSSVSDTIASAFSPASLIARGVRQMAPRPKFSAKKPAATRKQSRSLSLPQRLNSLPSPGLLSPLSTPGVQTVFSPIQRQTSFHRKPLSPWEAASRSPIGSVDEAFVFQNPMFSIASGIMAAAHHRSLPEPPEEWKRRVSLDSAAVRKGHYDCVKSQRCVMSSPKGQKQRNSFLKWGNLHSQEHPSGNPHPIIYSPLLTILASWCLRVALSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7U3</accession>
    <name>A0A3B5L7U3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane protein 199</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027854637.1">
        <property type="nucleotide sequence ID" value="XM_027998836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L7U3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005902.1">
        <property type="protein sequence ID" value="ENSXCOP00000005836.1"/>
        <property type="gene ID" value="ENSXCOG00000004527.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114133163"/>
    <dbReference type="KEGG" id="xco:114133163"/>
    <dbReference type="CTD" id="147007"/>
    <dbReference type="GeneTree" id="ENSGT00390000014591"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021013">
        <property type="entry name" value="ATPase_Vma12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31394">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31394:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11712">
        <property type="entry name" value="Vma12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="199" mass="22170" checksum="2B7282126DA0E980" modified="2018-12-05" version="1">MASSFVVGDKFRSRVTKLLGTPDSSLPEGLKEELKEILKKTQPTVLPFSTARKLKKHLQDKGHPFYLHQLLEDSSLHLPEVVKPPRNPELVARLEKIKAKLANEEYNRMTRNVNTQEINRNGTLSDFGQQVRLVKAVVVTVFNFLVTLVAAFACSYMGSQYLFTETAARVISAVIAASVVGLAELYVLVRTMEGELGEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9W5</accession>
    <name>A0A3B5L9W5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calcitonin peptide-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9W5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007369.1">
        <property type="protein sequence ID" value="ENSXCOP00000007275.1"/>
        <property type="gene ID" value="ENSXCOG00000005631.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177875"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021116">
        <property type="entry name" value="Calcitonin/adrenomedullin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00214">
        <property type="entry name" value="Calc_CGRP_IAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017254915" description="Calcitonin peptide-like domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="28"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR621116-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="74" mass="8370" checksum="EEB9D86C1C571536" modified="2018-12-05" version="1">DEILLCLCFILFLSVSIKTLHHLQKRMCKFSTCHRERLAHKLSVLAGKSGKKTTDVGKNSFGKREIHLPPAQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAP7</accession>
    <name>A0A3B5LAP7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">A2ML1 protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000007907"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008005.1">
        <property type="protein sequence ID" value="ENSXCOP00000007907.1"/>
        <property type="gene ID" value="ENSXCOG00000006067.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166660"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02897">
        <property type="entry name" value="A2M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000001">
        <property type="entry name" value="CD109 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1930:FF:000001">
        <property type="entry name" value="CD109 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041813">
        <property type="entry name" value="A2M_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019742">
        <property type="entry name" value="MacrogloblnA2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041555">
        <property type="entry name" value="MG3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040839">
        <property type="entry name" value="MG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF160">
        <property type="entry name" value="ALPHA-2-MACROGLOBULIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17791">
        <property type="entry name" value="MG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17789">
        <property type="entry name" value="MG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="9">
        <location>
            <begin position="403"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin" evidence="10">
        <location>
            <begin position="658"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="11">
        <location>
            <begin position="1273"/>
            <end position="1357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1364" mass="151239" checksum="4C12540669998A86" modified="2018-12-05" version="1">KQETLCAHVQNPAERLSLAVSLTVNSSSSVILEATVSEEFYHCLSFQVPAVPVRTVAVIQVTLAGEGASMSKKTKIVIEPPGFIHIVQTDKPIYKPGQTVKFRIVSLDASFIPVVRDPNSNRIAQWLNQTIGGGILDLSHASIPEAAQGTYTISVVTDKEEEISHTFDIKEYVLPKFEVKVDLPSVITILDTTTTFKICGKYTYGKPVLGRVKAEFCRKYFQFFWRSEEPDNDICKNFDLTLDKSGCASETMSLAEFALNKFSYSDRFELNAELEEDGTGVVLKGSGMASLSSQIRTVTFEDVPGAYKPGIPFEGKIKLVGPDSQPVANELLYLFVEDSKKMMLTTDAKGIAQFSLDTSEWKSSVSLKDTKENEPYVPNLRRPDYGLAYQFVSPAYSKSNSFLMLKHISEGFACDRDATVTAQYIIQGTEVKEGQTVLNFFYLVLNVKTGTGELTVRLSEVIDLAPVAQVIVYSVMANGEIVADSQDFPIQLCLSNKVSLKFSSVQQLPAEQTTLTLKANPKSLCSVRAIDQSVLLLKQEQELNVDYVFNQLPVQKLSGYDYEVEDFDPFRCFPGMIPRPIPEFQPVPERSLFIPPFYQKIDVYSIFKVKIYWNVFLLFLEVGLAGPVAFNMALARGGEARGPEEQKKETVRTEFPETWIWDLITVGDGGSASLQKTVPDTITKWAASAFCVSPTGFGVSPNTGLTAFQPFFVSLTLPYSVIRGEVFTLKATVFNYLSQCIMVKVTLAQSDQFTFRDCEGCQYSICLCGEESKTFSWIVTPTALGDVSLRVSAEALRTRDLCGNEVTTVPDVGRIDTVVRTLLVEAEGTPQMKSHNALLCPADGPVEKKISLHRPEMFVEGSARASVSVLGDLMGRALQNLDKLLAMPYGCGEQNMVLFAPNIFILNYLKSSGQLTETILEKAKRFLESGYQRELNYKHDDGSYSAFGNSDPSGNTWLTAFVMKSFGGARDHIYVDPQHIKDARTWLSRLQKSDGCFTSVGKLFHNSMKGGVSDDVSLTAYIVAAMLELDGVASDPVVQKGLRCLKDAVAGEFDNLYTTALMTYTFSLARDQEMRSKLVTILDQKAKKDGRKKSRVGSSEAGLDAVEVEMTSYVLLALLLSPPLEGFSLDYTSGIIRWLTQQQNPYGGFSSTQDTVVALQALARYAEATYSPQGMTTVTVTSVGGLRKEFTVTQSNRLLYQEEELSEVPGEYTVRAEGHSCVLTQISAHYNVPPPDDFSAFTITKSIEPRCKLSRPQLHLAVHVRYQGRRDETNMVIINIKLLSGYVLDQSSLQLRSVKRVDSDEGFLNIYLDGLKKDEPQTCKVTLEEDQRVRNLKPAVIKVYDYYQTLDGCIDYPFATAALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LB65</accession>
    <name>A0A3B5LB65_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LB65"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008077"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008175.1">
        <property type="protein sequence ID" value="ENSXCOP00000008077.1"/>
        <property type="gene ID" value="ENSXCOG00000006204.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005094"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040893">
        <property type="entry name" value="RADX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14944">
        <property type="entry name" value="RPA-RELATED PROTEIN RADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14944:SF4">
        <property type="entry name" value="RPA1 RELATED SINGLE STRANDED DNA BINDING PROTEIN, X-LINKED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17659">
        <property type="entry name" value="RADX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="522"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="718" mass="81365" checksum="328C2AA26DB55E62" modified="2018-12-05" version="1">MESSSRCSSMAAGLPRESFLQRTLERLPSTQSLKLTAEEAAPVAVVAVQRYLSEPTEGQQLDSYSYDVTITDGVWRAKCFLHPSLNHLVHRNTLRTGTDVSVRQCSFVYNERRLGHGYICIEKVEGSAETSAVLQSDVPLQVSRKHYLSLWNNDDPEGDMWISGRSEILIICNIFISVSKVTLLSSLESSFRVSWKPLPLLVKIIHKSRLRYYGKFGLKIDYPYQTYFEVADQSGTMSLVLWNELCPEYYLRMNVGAVLYLQNYTLKQSYSNRSRPQMDHHRMKNFTSIEICLNPRNPAADITVVSPKSVLPQWGLPEVSYQFTTRSELEKISNGSACDVIGLVTFVGRVERVRSKGSKEKYWTYRWVHSVDGTSDRPFILELFSSSQPEIFSQICPMTYLVCTQMRVCQAEGSLPYLTSSCETEIFITGYHKGQPYVSDPRVKSFIQWTKTLKDNVVLQKTAVGGHYCYPPPPRRFSQSAGDLSDLFIDFKESLQYREHKKLAIQGEITAIRYTNYPKKVSNEPKNYSSDTHKDPADYSAAPSWESSSWSKQREEISEHLHHGGLHQDSISRRFTFDEKSALSLSFQCVRSQHGPLILVRIFFYVFLSGINKQIAVDAAFLPVVRSDDPRAVGLSRDPHDNSMRNKSLLSSSPASPVERLALISLTFGCLLAEEILATARGLENTHVVCVLELCQLGGDKVEILIDKVYKVMDVTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBQ9</accession>
    <name>A0A3B5LBQ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ShKT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBQ9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009623"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009737.1">
        <property type="protein sequence ID" value="ENSXCOP00000009623.1"/>
        <property type="gene ID" value="ENSXCOG00000007332.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156439"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.740:FF:000001">
        <property type="entry name" value="Cysteine-rich secretory protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000005">
        <property type="entry name" value="Cysteine-rich secretory protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.740">
        <property type="entry name" value="Crisp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042076">
        <property type="entry name" value="Crisp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013871">
        <property type="entry name" value="Cysteine_rich_secretory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003582">
        <property type="entry name" value="ShKT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08562">
        <property type="entry name" value="Crisp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57546">
        <property type="entry name" value="Crisp domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51670">
        <property type="entry name" value="SHKT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="ShKT" evidence="5">
        <location>
            <begin position="184"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="193"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="202"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="25040" checksum="6F88C1D2B7A34DF6" modified="2018-12-05" version="1">MCVCACWRVCTDVCPENTIVQAEIVNVHNAFRRAVEPAAADMLMMTYSLELAASAQAWVDKCTLSHGPASTRLLDGYELGENLFSSSSPYSWTTVVTTWHNEKSRYLYPNGSTNGKPIGHYTQVVWNSSYKVGCGLALCPNNIYYYGCHYFRAGNFKHWPPYKVGTSCASCPNNCVDKLCFNPCPYVNTFINCPRLKDVKGCSNPLVFARCPASCKCTTEIVPIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBZ5</accession>
    <name>A0A3B5LBZ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBZ5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008367"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008473.1">
        <property type="protein sequence ID" value="ENSXCOP00000008367.1"/>
        <property type="gene ID" value="ENSXCOG00000006347.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286954"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010644">
        <property type="term" value="P:cell communication by electrical coupling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Gap junction alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF33">
        <property type="entry name" value="GAP JUNCTION ALPHA-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0303">Gap junction</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="209"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="13">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="14">
        <location>
            <begin position="165"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="251"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="275" mass="31500" checksum="D656BF0457B1724F" modified="2018-12-05" version="1">MGDWSALGRLLDKVQAYSTAGGKVWLSVLFIFRILVLGTAVESAWGDEQSAFKCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSTPTLLYLAHVFYLHRKEQKLNRKEEELKAVQNDGGDVDLPLKKIEMKKMKYGIEEHGKVKMKGALLRTYIVSIFFKSLFEVGFLVIQWYMYGFTLSAVYTCERSPCPHRVDCFLSRPTEKTVFIIFMLVVSLVSLLLNIIELFYVFFKRIKDRVKGKQQPTLYASGGTLSPTPKELSTTKEMSPSRFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCI1</accession>
    <name>A0A3B5LCI1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transient receptor potential cation channel, subfamily V, member 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCI1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007406"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007502.1">
        <property type="protein sequence ID" value="ENSXCOP00000007406.1"/>
        <property type="gene ID" value="ENSXCOG00000005711.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158512"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:calcium channel activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
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        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024862">
        <property type="entry name" value="TRPV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008347">
        <property type="entry name" value="TrpV1-4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10582:SF5">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL SUBFAMILY V MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10582">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL ION CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01768">
        <property type="entry name" value="TRPVRECEPTOR"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
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            <end position="165"/>
        </location>
    </feature>
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            <end position="190"/>
        </location>
    </feature>
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        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
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            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="8">
        <location>
            <begin position="72"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="457" mass="53489" checksum="5DF17DF0CA46E73A" modified="2018-12-05" version="1">FKHLSRKFTEWAYGPVHSSLYDLTSVDSYENNSVLEILIYGSEIPNRHEMLEKEPLSCLLEDKWKKFGRGMFFFNFLVYLVYLIIFTVVAYNKKDLPFIPEDAWDYLYVSGQLLTLLANCYFFVTGIIEMKRKSPKMQTLLIDGYYEILFFAQGLLFLVATVLYWAGRQEYLGFLVISLALSWVNMLYYSRGDKHMGIYSVMIQKMILSDILRFLFVYIVFLFGFSAAVVTLLIQPPPENRTGKGRQGFFATSKPDTECFTPSYRNISYTTLQLFKFTIGMGDMEFTQEYQYMEVFYVLLIGYIILTYILLLNMLIALMSRTVEKITTESASIWRLQVSDWHSFWYLLKRAVVTWVAHASPCLLRVEEMNWNKWNTNLVNISEDPGCCDLSQQPDLDSPSRGFNRGCSKKKKSPRLYINPDLVIFPCFSTGRSWRDIFMEGSWRRRRPPQSTEMSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCL9</accession>
    <name>A0A3B5LCL9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Macro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCL9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008698.1">
        <property type="protein sequence ID" value="ENSXCOP00000008592.1"/>
        <property type="gene ID" value="ENSXCOG00000006577.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006988"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140291">
        <property type="term" value="P:peptidyl-glutamate ADP-deribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02901">
        <property type="entry name" value="Macro_Poa1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050892">
        <property type="entry name" value="ADP-ribose_metab_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12521:SF0">
        <property type="entry name" value="ADP-RIBOSE GLYCOHYDROLASE OARD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12521">
        <property type="entry name" value="PROTEIN C6ORF130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="6"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="169" mass="19289" checksum="2BC248359C052CAA" modified="2018-12-05" version="1">YLIINETLTSRLDEYKTHLNYVTGDLFSCPRDESLAHCISEDCRMGAGIAVMFKKKFGRVSELKEQKKLPGQCAVLTHDQRFIYYLITKKKASQKPTYVNLRQSLEDMKSHCLENGVNRISIPRIGCGLDQLLWSKVSKILEQIFKETNISITVYSLPCVGSKLQMHAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDN7</accession>
    <name>A0A3B5LDN7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Roundabout, axon guidance receptor, homolog 2 (Drosophila)</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDN7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007801"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007899.1">
        <property type="protein sequence ID" value="ENSXCOP00000007801.1"/>
        <property type="gene ID" value="ENSXCOG00000005990.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156324"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000053">
        <property type="entry name" value="Roundabout guidance receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000055">
        <property type="entry name" value="roundabout homolog 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000065">
        <property type="entry name" value="roundabout homolog 1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000008">
        <property type="entry name" value="roundabout homolog 2 isoform X2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000043">
        <property type="entry name" value="roundabout homolog 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000058">
        <property type="entry name" value="roundabout homolog 2 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051170">
        <property type="entry name" value="Neural/epithelial_adhesion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231">
        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12231:SF242">
        <property type="entry name" value="ROUNDABOUT HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="13"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="105"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="192"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="296"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="403"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="516"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="618"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="901"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="922"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="952"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="963"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1000"/>
            <end position="1016"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1120" mass="122226" checksum="E4C5E4C917A7BE44" modified="2018-12-05" version="1">SVLHLLLILVLRDDFRQNPQDVVVAVGETANFECQPPRGHPEPTIFWRRDKTQLDLKDDRVMVRGGKLTISNTKKSDSGIYVCVASNMVGERESEKAQLSVYERPAFMQRPVNQVVLVDESVEFRCQVHGDPPPALHWKKEDVDVPRGRYDREDSLLRIKKASVSDQGTFTCVAENRVGKAEASAYLTIRAPQFVVRPRDQIVAQGRTATFPCVTRGKPQPTVFWQREGSKDLLFPNQPVQGDDRVSVSVNGELTISSVQRSDAGYYICQALTVAGSIMAKAQLEVADASKGRPPPIIRQGPSNQTQALGGVSILQCQASGDPEPTVTWRKNGANLLGQDPRFSLLEHGSLQIQNTRLSDSGLYTCVATSSSGETSWSAYLDVKDSTDLMDFMSHNTTALPGPPSKPEVTDVTKSSISLSWEPGPEAGSPVSSYVIEAFGQSVSNSWQTVADHVKTTEFTVKDLRPNTVYLFIIRAVNAQGLGDPSPMSEPVRTQDISPTVQGVDHRRVQKELGDVVVNMHNPVVLSSTSVQVTWTVENPSQFIQGYRVLYRQTSGLPSPGPWQIQDMRIASERDVTLAGLNKGIVYEIKVRPYFNEFQGADSESMTARTMEEAPSAPPQQVAVLTVGSHNSTSISVSWDPPPSDQQNGIIQEYKIWCLANDTRLHVNKSVDATIRSVVVGGLQAGVQYRVEVAAGTSAGVGVRSKPQLIILGSEGNNSISDVVKQPAFIAGLGGACWIVLMGFSAWLYWRRKKRKGLSNYAVTFQRDRGLIRNGSRPGLLKAGDPGLPWLADSWPSTSLPANGGLGSPKGGNNFGRGDVLPSAAVEKTGTMLSDGAIYSSIDFMGKGGYSSPARGVQPTPYATTQILQSNSFHELAVDRPDPRWKASLQAQQEMANMGYSLTDRRPGAGAKVAKKKKIKGGTKTSKSNGTCWANVPLPPPPMHPLPGTEVDLDRYPQENHKGSSNSWAPPMSIQTYRHQDLDSEAEEERGPTPPLRGRSSSPAPFSQPSSSSLSSAHHEEMQSILQAHLDELTRAYQYEVAKQAWHMKSSANVSNTPMDFMSSTLGSDLGATLLSEDDEEEEEEEDERYAVVSKNLCGFEYTPGHTVDSFEGTGMCSYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LED2</accession>
    <name>A0A3B5LED2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cyclin-dependent kinase</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LED2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010518"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010641.1">
        <property type="protein sequence ID" value="ENSXCOP00000010518.1"/>
        <property type="gene ID" value="ENSXCOG00000007959.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156963"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07844">
        <property type="entry name" value="STKc_PCTAIRE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000007">
        <property type="entry name" value="Cyclin-dependent kinase 14, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000061">
        <property type="entry name" value="Putative cyclin-dependent kinase 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF499">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 16-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="90"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="397"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="420" mass="47779" checksum="D3A14CE6A53B98E1" modified="2018-12-05" version="1">MGSDGESDQASGTSSDEVQSPVRVRMRNNHHRRISNEDINKRLSLPADIRLPEGYLEKFAMNSPPFDKPMSRRLRRASLSEIGFGKLETYIKLDKLGEGTYATVFKGRSKLTDNLVALKEIRLEHEEGAPCTAIREVSLLKDLKHANIVTLHDIIHTDKCLTLVFEYLEKDLKQYMDDCGSIMSIHNVKIFLFQLLRGLAYCHRRKVLHRDLKPQNLLINEKGELKLADFGLARAKSVPTKTYSNEVVTLWYRPPDVLLGSTEYSTPIDMWGVGCIFYEMITGRPLFPGSTVEDELHLIFRILGTPTEATWPGITTSEEFKTYNFPRYHAEPIVNHAPRYSKTACVLLVKVLFEAKNRVSAEEALRHPYFKTLGEHVQSLPDTASIFSIKGIQLHKDPGKRSSLHPESSQGKNRRQSVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHC6</accession>
    <name>A0A3B5LHC6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Neuropilin (NRP) and tolloid (TLL)-like 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHC6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010262"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010385.1">
        <property type="protein sequence ID" value="ENSXCOP00000010262.1"/>
        <property type="gene ID" value="ENSXCOG00000007784.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156700"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000463">
        <property type="term" value="P:positive regulation of excitatory postsynaptic potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000013">
        <property type="entry name" value="Membrane frizzled-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000016">
        <property type="entry name" value="neuropilin and tolloid-like protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF27">
        <property type="entry name" value="NEUROPILIN AND TOLLOID-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="38"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="169"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="289"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="308"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="532" mass="59682" checksum="F0AEA2EA180022D2" modified="2018-12-05" version="1">LSLSSGSPAIAASLIPPELCGSTANTKKAQGVTPAGQCGTWVKEPSGGCFTSPNYPEKYPPERECIYIIEASPRQCIDLFFDEKYSIEPSWECRFDHIEIRDGPFGFSPIIGRYCGQESPAYVRSSGRYLYIKFVADGELEAIGFSARYNFTQDPEFEGIGELSALPACDFELSGPEGFVESTQIAKEGKAQLTEAVDCRWYIRAPPRAKIYMRFLEYEMHNSNECKRNFVAVYDGSSGVEHLKNKFCSTVANDVMLVTSVGVVRLWADEGSRKSRFKILFTTFHEPPCEGDTFFCHSNMCINHTLVCNGVQNCVYPWDENRCKEKRKASILDTLDNTNLTIIGITCGLVVILLIISVIIQIKQPRKKYVIRRDDFDPALLHPGFDPPHYELCTLRRAPSGDLTDAALADDFDKFHKLRRSESKCIRDHHCGSHQGSIHGSRSNLSLRDATIALATQMSPHLSHHNTPACRRSILVMKHSYSQDGAEAYRAEDEEDDLMMDVGPTTSQHHHHHIHHKLSSVLNTTLLMAQMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHH4</accession>
    <name>A0A3B5LHH4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Insulin gene enhancer protein ISL-1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHH4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011583"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011715.1">
        <property type="protein sequence ID" value="ENSXCOP00000011583.1"/>
        <property type="gene ID" value="ENSXCOG00000008741.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153731"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072359">
        <property type="term" value="P:circulatory system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031017">
        <property type="term" value="P:exocrine pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048665">
        <property type="term" value="P:neuron fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09366">
        <property type="entry name" value="LIM1_Isl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000034">
        <property type="entry name" value="Insulin gene enhancer protein ISL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000041">
        <property type="entry name" value="insulin gene enhancer protein ISL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047169">
        <property type="entry name" value="ISL1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047244">
        <property type="entry name" value="ISL1/2-like_LIM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24204">
        <property type="entry name" value="INSULIN GENE ENHANCER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24204:SF4">
        <property type="entry name" value="INSULIN GENE ENHANCER PROTEIN ISL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9 15 17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 15 17" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 16" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 15 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="19">
        <location>
            <begin position="15"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="20">
        <location>
            <begin position="145"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="15">
        <location>
            <begin position="147"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="276"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="287"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="315" mass="35290" checksum="63A7B49D62391395" modified="2018-12-05" version="1">MGDMGDPPKKKRLVSLCVGCGNQIHDQYILRVSPDLEWHAACLKCAECSQYLDESCTCFVRDGKTYCKRDYIRLDKADAAVRNRLQSYHSSGRTALLYGCGFWMFIRPISYLGLILPLSTLCFQTAEPISARQPALRPHVHKQPEKTTRVRTVLNEKQLHTLRTCYNANPRPDALMKEQLVEMTGLSPRVIRVWFQNKRCKDKKKSLLMKQLQQQQPNDKTNIQGMTGTPMVAASPERHDGGIQANPVEVQSYQPPWKVLSDFALQSDIDQPAFQQLVSFSEGGPGSNSTGSEVASMSSQLPDTPNSMVSSPIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHH7</accession>
    <name>A0A3B5LHH7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Metalloendopeptidase OMA1, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Overlapping with the m-AAA protease 1 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homooligomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHH7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011720.1">
        <property type="protein sequence ID" value="ENSXCOP00000011588.1"/>
        <property type="gene ID" value="ENSXCOG00000008744.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007027"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07331">
        <property type="entry name" value="M48C_Oma1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051156">
        <property type="entry name" value="Mito/Outer_Membr_Metalloprot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726">
        <property type="entry name" value="METALLOENDOPEPTIDASE OMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726:SF1">
        <property type="entry name" value="METALLOENDOPEPTIDASE OMA1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M48" evidence="11">
        <location>
            <begin position="213"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="442" mass="49747" checksum="C6CE5CBEFD51AF0B" modified="2018-12-05" version="1">MAFLCGNLLRNTSSFYFHMNLTRHFQLCKRADFPLKKEKTPFSVCSPKISSNLRVCATRQSFPICGNVRTACSRIGSVQTQWGHHFHTSSSRRALPAPLLWMVLKPLQKLLAILLGRSLRKWWGSLPDNRRQLVHEWLRRRRWHLATGAGVALVIVALLLLTHLDESPVTGRIRLLVFSREKYMELAALTSEAHLEEFAELLLPVTDPLHQMVEQAVQLLAQRNKDIPEVSEVPWSVHVVESPQINAFVLPNGKVFVFTGMLGAVADLDQLMIVLGHEMAHAILDHSAEQASLSHVVDLLSLIVVTAIWALCPGDILAVVGGWAKDKLTEGVEAKVRMACADVRAGPVFWQQMELVDNLTGNPTLPEWLSTHPSHRNRSGQMDRLVPQNINKYLLVIQITLLYVTGVTKECISNGNVLQELHLCLWGRDRCVMPSQLFSYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJ86</accession>
    <name>A0A3B5LJ86_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C-type natriuretic peptide 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Exhibits natriuretic and vasodepressant activity. Has cGMP-stimulating activity. May help to regulate body fluid homeostasis in a variety of aquatic environments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the natriuretic peptide family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027866588.1">
        <property type="nucleotide sequence ID" value="XM_028010787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJ86"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010952"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011077.1">
        <property type="protein sequence ID" value="ENSXCOP00000010952.1"/>
        <property type="gene ID" value="ENSXCOG00000008269.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114140658"/>
    <dbReference type="KEGG" id="xco:114140658"/>
    <dbReference type="GeneTree" id="ENSGT00500000046195"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097746">
        <property type="term" value="P:blood vessel diameter maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006182">
        <property type="term" value="P:cGMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007168">
        <property type="term" value="P:receptor guanylyl cyclase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000663">
        <property type="entry name" value="Natr_peptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030480">
        <property type="entry name" value="Natr_peptide_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002408">
        <property type="entry name" value="Natriuretic_peptide_brain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12167">
        <property type="entry name" value="C-TYPE NATRIURETIC PEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12167:SF6">
        <property type="entry name" value="C-TYPE NATRIURETIC PEPTIDE 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00212">
        <property type="entry name" value="ANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00712">
        <property type="entry name" value="BNATPEPTIDE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00710">
        <property type="entry name" value="NATPEPTIDES"/>
    </dbReference>
    <dbReference type="SMART" id="SM00183">
        <property type="entry name" value="NAT_PEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00263">
        <property type="entry name" value="NATRIURETIC_PEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0372">Hormone</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="7 9" id="KW-0838">Vasoactive</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017364300" description="C-type natriuretic peptide 2" evidence="10">
        <location>
            <begin position="22"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="13833" checksum="6E5C6ED6BDFC0A9E" modified="2018-12-05" version="1">MAAASSAPVGLLLLLLVSVETRPSPQHEQRVFLSLFGSRLASLMEATPPPDDITEGSASSPTSPPLLLALSRHGNVSGLAGRQEVVPRLFLDFLRWRAKTRRRSHKATAGGRGCFGMKLDRIGSISGLGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJB2</accession>
    <name>A0A3B5LJB2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein strawberry notch homolog 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Acts as a transcriptional coregulator, that can have both coactivator and corepressor functions. Inhibits the DCSTAMP-repressive activity of TAL1, hence enhancing the access of the transcription factor MITF to the DC-STAMP promoter in osteoclast. Plays a role in bone homeostasis; required as a positive regulator in TNFSF11//RANKL-mediated osteoclast fusion via a DCSTAMP-dependent pathway. May also be required in the regulation of osteoblast differentiation. Involved in the transcriptional corepression of NF-kappaB in macrophages. Plays a role as a regulator in the pro-inflammatory cascade.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with TAL1; this interaction inhibits TAL1 occupancy of the DCSTAMP promoter, leading to the activation of the DCSTAMP promoter by the transcription factor MITF.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SBNO family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSXCOT00000012314.1">
        <property type="protein sequence ID" value="ENSXCOP00000012173.1"/>
        <property type="gene ID" value="ENSXCOG00000009209.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159946"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071354">
        <property type="term" value="P:cellular response to interleukin-6"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002281">
        <property type="term" value="P:macrophage activation involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000342">
        <property type="entry name" value="Protein strawberry notch homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003990">
        <property type="entry name" value="Si:ch73-63e15.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026937">
        <property type="entry name" value="SBNO_Helicase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026741">
        <property type="entry name" value="SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039187">
        <property type="entry name" value="SNO_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706:SF5">
        <property type="entry name" value="PROTEIN STRAWBERRY NOTCH HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12706">
        <property type="entry name" value="STRAWBERRY NOTCH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13872">
        <property type="entry name" value="AAA_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13871">
        <property type="entry name" value="Helicase_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0892">Osteogenesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="181"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="518"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1236"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1017"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="95"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="518"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="530"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="551"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1236"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1251"/>
            <end position="1261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00063805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1374" mass="152425" checksum="9601E8BCC82DF9E3" modified="2018-12-05" version="1">FPAVFSTSIWLTVISPPKSCDLSCIDDLSNSLFSSPADSLSEYADTQSYIPSNHPDPVPTLWDINTSPATTQAQSHLEVMFDLILPSTPVTQPPPEEEEEAEEEEPEELGHVDTYAEYKPSKSTIGISHPDIVVETNTLSSVPPPDITYTLSIPDSTINSGLLSALQLEAIIYACQQHEVILQNNQRAGFLIGDGAGVGKGRTVAGIILENYLKGRKKALWFSISNDLKYDAERDLKDIDAPNIPVHALNKIKYGDTATSEGVLFATYSALIGESQAGGQHRTRIKQILDWCKQDFDGVIIFDECHKAKNATSTKMGKAVLDLQNKLPHARVVYASATGASEPKNMIYMSRLGIWGEGTPFRTFDDFLHAIEKRGVGAMEIVAMDMKVSGMYIARQLSFSGVSFRIEEIGLDSDFKEVYNKAAKLAADELGLVSRKSLWGQFWSSHQRFFKYLCIAAKVRCLVELAQKELEAGKCIVIGLQSTGESRTREVLDENDGHLDRFVSAAEGVFQSLVTKHFPSEKQRREKAPGNKRKKGKPRGRQAKMPKHTVDSGGVINISDDSSSDSEGMDTDSNSSPDSLQDNDDVIFVNHTSHQSGRAFDRQCLSTVALQTGLLNKIAVLGKELPLNTLDVLIDRFGGPDKVSEMTGRKGRVVRRPDGTVRYESRAEQGSTIDQINIKEKDRFMCGEKLVAIISEAASSGISLQADKRVKNQRRRVHMTLELPWSADRAIQQFGRTHRSNQVTAPEYIFLISELAGERRFASIVAKRLESLGALTHGDRRATESRDLSKYNFENKYGTKALDKITKSINGYIENKVPPPKGYPEGDAMFFRDMKVGMMDVGIICKEPRFGISTEKDCSITKFLNRILGLEVHKQNYLFQYFTDNFDYLIEKDKKEGKYDMGILDLAPGNDEIYEEKQETFLTVGNPQDGQVVLYKVGTAQPHLRCVMVYVSCETLVSVIFKDFPMLKILENRNIFLSIVFSRFQKCLVFCLHSFDSLVSFSPLLLHTGIKIPENCVARVREELLQMDEEVKRRRKEKEQQERMRKMEQENKHLLENLFSRKPIQNQTLSHPLVSNPFPKQNLNALQRTQLGSFSQPSSHSPSLFPLNGWPNNSSSLSSPSSATNGQVCQPSHRFPPFYGSSSFMSFPQSKNPPLQLTQFSPAPLLSSKNPLDEILDLTVSPPSSSPDSTTANPAAFVEDFNLDVLLGQTAPDMLTAPQTQLPQLDLQHILQHTSETAATPSSQFDLQRAPEPQQEQQTQEQSRDLLVNNHQDILELLCAGLSPLPSAQKAVSSSSALSHYPNPPSSSSSSSSSSLYSSAAHSSCFPANSLLPLSDPLPNGQCGAGALDVREALNSMLQAGPDRKSVIQYRQQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJB5</accession>
    <name>A0A3B5LJB5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Calmodulin regulated spectrin-associated protein family, member 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="6">The CKK domain binds microtubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CAMSAP1 family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSXCOT00000011109.1">
        <property type="protein sequence ID" value="ENSXCOP00000010982.1"/>
        <property type="gene ID" value="ENSXCOG00000008297.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182975"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036449">
        <property type="term" value="C:microtubule minus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030507">
        <property type="term" value="F:spectrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.360:FF:000001">
        <property type="entry name" value="Calmodulin-regulated spectrin-associated protein 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.360">
        <property type="entry name" value="CKK domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032940">
        <property type="entry name" value="CAMSAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031372">
        <property type="entry name" value="CAMSAP_CC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058042">
        <property type="entry name" value="CAMSAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022613">
        <property type="entry name" value="CH_CAMSAP_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014797">
        <property type="entry name" value="CKK_CAMSAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038209">
        <property type="entry name" value="CKK_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595:SF1">
        <property type="entry name" value="CALMODULIN-REGULATED SPECTRIN-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21595">
        <property type="entry name" value="PATRONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17095">
        <property type="entry name" value="CAMSAP_CC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11971">
        <property type="entry name" value="CAMSAP_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08683">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25532">
        <property type="entry name" value="CH_CAMSAP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01051">
        <property type="entry name" value="CAMSAP_CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51508">
        <property type="entry name" value="CKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="9">
        <location>
            <begin position="211"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="CKK" evidence="10">
        <location>
            <begin position="1228"/>
            <end position="1362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="518"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="630"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="725"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="787"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1062"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1122"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1173"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="191"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="674"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="637"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="830"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="853"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="897"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="911"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="987"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1013"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1130"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1177"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1215"/>
            <end position="1224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1365" mass="152096" checksum="17FFECCB72BA356A" modified="2018-12-05" version="1">MGDTAEDRGMRRTFIVPAIKSFDHYDFTRAKISCSLTWLVAKAFGSDAVPEELAEPFYRDQYNQEHLKPPVACLLQSAELYCRAGSLILRSDAVKPLLGHNAVIQALAQKGLYVTDQDRLVTERDLTSMPIHMSSHLALIDTLMMAYTVEMVSVERVMSCINKYSSAELLHSDGLVQGLPYETEDAIATWINKVNEQLRDILIEEQKLREASLQDSNARYKREHAQKNVPSLPLVENLLKDNAGGCALAALLHFYCPQAVRLEDICLKETMSLADSLYNLQLVQEFCRNNLNHCCHFSLEDMLYTHASIKSNYLVFMAELFWWFEVVKPSFVQPRVFDPNGTIQSVPVKQNYADRPTSPENLTTKKVPLCVTGLMKRSTSMSYVDGCVGTWPKEKQSSSRGISFEIPLDGDATLPLFEAPSTCGMTRSASTDGLGFKVHYTSRGGMKRHLSLTPVDVNGQGRQIPEEDDNFASHKPLGRNNTFSIKSQSSIEEALKIIHDTERPHASLAVGDGDNGFFLHGAESSDPPGAQAPGDDFGSAKARVNDRDLNSASTDEVDTGIHVRTEDIQSLDEDSSSLRDYSDIDPDCEAMTRSCPLPGKQERSSPTSSSGESGGGLVRMTSFAEQKFRKLEGRSSGGTTTPDSSDLNFSTLPLPITSPSLATPSPRDPSHFIASEMIQLRMKLEEKRRAIEAQKKKVEAAFTRHRQKMGRTAFLNVVKRKGVTAPLSQNSGGAETPSPEPISSSKEDSMTAGEVDLAEYTRSIERLNTSLGFLQTEMQRLAQQQEKIMAMRDQQQQAWVIPPPAPSPHRQLRELRSSSVTGRGSGRGSVGSLSPILSSSGSPRAPNRSPAGIKRRPASFHARTPRTPRPNDLKVTPISRMLNPPTSVDSLPRLRRFNSSQTQQSSFAYLSHDEGVRGRHNQELKGEKDDSKKKEEMVVKSSDEEKCKEKTSERLKERTESKEESTTSEVLCQPVSEIKGGVVSQSQDDKQERKDLVEVPLSGVKPPGGRGPKNTEEGEKGGESYGDDQKMCCGFFFKDDVKGEEDMAAKKAALLEKRLRREKEAQERRQQQELDQEQKKEAARLKAEEEQQKKDEEKARREYIKYEYLRRKQLKLMEDMDEVIKPRSGSLKKKQRPKSIHRDIMESSTPPVRTTGVRPRGFSVSSVSLASLNLADNNRDQPDNRKNNRPDSAEGFSSCPLAGSRNGEKDWENDSTTSSTPSNTEYTGPKLYKEPSARSNKHIIQNALAHCCLAGKVNEGQKNKILDEMEKSEANNFLVLFRDSGCQFRSVYTYCPETEEIAKLAGIGPKSITPKMIEVLYKYNSDKKQFSKILAKTMSASVDAIAIANHLWQTKKQGTPKKHPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJL5</accession>
    <name>A0A3B5LJL5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Collectrin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJL5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011092"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011219.1">
        <property type="protein sequence ID" value="ENSXCOP00000011092.1"/>
        <property type="gene ID" value="ENSXCOG00000008379.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160862"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051957">
        <property type="term" value="P:positive regulation of amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042944">
        <property type="entry name" value="Collectrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031588">
        <property type="entry name" value="Collectrin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46884">
        <property type="entry name" value="COLLECTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46884:SF1">
        <property type="entry name" value="COLLECTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16959">
        <property type="entry name" value="Collectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52010">
        <property type="entry name" value="COLLECTRIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Collectrin-like" evidence="11">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="149"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="189" mass="21225" checksum="4A7EEA77C7DD829D" modified="2018-12-05" version="1">EGFKVRLSIKTALGDKAYEWNRNQRFLFQAAIAFAMSHHLDLEFNVSNVLVCDETPRVSFWFVVTSPGDPSRLVGKESVELAVRESRDRINNVLLLNDATLEFVGVLPTLVAPVKPATPPWLIVFGVVIGIVGAGIVFLLVSTVVQKKRKEKEVAEEEEDDEEAQMKRTARNEGIYNTSFSEDDRNTQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LK23</accession>
    <name>A0A3B5LK23_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">E3 ubiquitin-protein ligase Midline-1</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">RING finger protein Midline-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">RING-type E3 ubiquitin transferase Midline-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Tripartite motif-containing protein 18</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 24">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LK23"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011000"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011127.1">
        <property type="protein sequence ID" value="ENSXCOP00000011000.1"/>
        <property type="gene ID" value="ENSXCOG00000008306.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155821"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070507">
        <property type="term" value="P:regulation of microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003649">
        <property type="entry name" value="Bbox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017903">
        <property type="entry name" value="COS_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050617">
        <property type="entry name" value="E3_ligase_FN3/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040859">
        <property type="entry name" value="Midline-1_COS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099:SF23">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MIDLINE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18568">
        <property type="entry name" value="COS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00502">
        <property type="entry name" value="BBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51262">
        <property type="entry name" value="COS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="COS" evidence="21">
        <location>
            <begin position="105"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="166"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="19">
        <location>
            <begin position="251"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="452" mass="50899" checksum="88274F9C6E2B096C" modified="2018-12-05" version="1">RCSHRETLDSNLSNLIKRNNELESLMGKLIQTCQHVEVNASRQEGKLLEECDVLIDIIQQRRQIIGSKIKEGKAQRLRKLAQQISNCKQCIERSSALITQADQTLKETDHARFLQTAKSINERVSMATASTQVLIPEIHLTDTFDTFALDFTREKKLLENLDYLTAPSAPCIREELCTASYDTISVHWTSDDEFTVVSYELQYAIFTGQSNIASLCNSLESWMIVPNIKQNHYTVHGLQSGTKYIFVVKAINQAGSRSSEPGTLKTNSQPFKLDPKSAHKKLKVSHDNLTVERDETTSKKGHSQDRFTSQSSYGVVGNVYIDSGRHYWEALIGGSTWYAIGIAYKSAPKHEWIGKNSASWVLCRCNNSWVVRHNSKELAIEPSPQLRRVGVLLDYDAGYVAFYDAVGSQHLHTFHVSFIQPVCPVFNVWNKCLTILTGLPIPDHLEGLEPGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKN1</accession>
    <name>A0A3B5LKN1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Kinectin 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKN1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012633"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012785.1">
        <property type="protein sequence ID" value="ENSXCOP00000012633.1"/>
        <property type="gene ID" value="ENSXCOG00000009493.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158237"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024854">
        <property type="entry name" value="Kinectin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007794">
        <property type="entry name" value="Rib_rcpt_KP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18864">
        <property type="entry name" value="KINECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18864:SF1">
        <property type="entry name" value="KINECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05104">
        <property type="entry name" value="Rib_recp_KP_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome receptor lysine/proline rich" evidence="9">
        <location>
            <begin position="31"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="46"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="550"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="591"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="306"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="365"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="773"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="550"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="600"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="994" mass="112383" checksum="80ED829DF00E359B" modified="2018-12-05" version="1">MAMDIYDSQYLLILAPTLVIALMFLFFWLFMKETSYDEVLARQKRDLKLPPTKPDSRKKNEKKKSKKKEAAGGGSGGGGGESEEDLRDFDLTDVIGSNVEAEEEPEPVVTPDPSPPAPAAEVSVSPETTSGLRERKKKEKKAAKAAAKAAAAAASNLEESEVNDSKPVSHKTETSPTSGKRMSPPPPQMETQVQVQAVQAPVQTQTPPQTSGKKKDRKKQKVESQAAHNDDIPPKASGKKQKNETDKNTEVKLKELLSGLSNLALSEAEAISVIALLREKNPSAVDAWHKSTARPDPAAQEREKLLVTLQEESSIAKDKVKQLSQELQLEKQKTGRMETMMREQRVAMEKELGGMQAKAQGSYQELQFQQVREQLEAQITRLQQENGILRDAVSSATTQMESRNSAELNKLRSEYAGLMKELADNNSKLQQEEHQRKSLEVSYKQNVSQLEQDAKRSWEELQNFLHNVNAEREKLQASKQLHSQLLAAESEMNNKNKELQTLHSNLNEAMLSKEQLERRVLELMEMSQRSVPDETVQAQVQELKNENKALQAQNEAQQSQNENLQAQISSQANHVSHIEELQKLSEKELQRKSLEDSLNAERSSGANRETNMQVQEKEENIKTVEGLLEKGLIEVANKEAKLKIQEKDVKLKSVEESLQAAQARSSTMEKTVEFSEKESQVGNLQVELAELKDALELHRKKNNELREKNWSAMEALSATESMLQGKLSKAVKESQATLAQTQAECRDVLHRLLPHVPLPSEQNHQEWLQRFETAVAESLLQELAEKLKEAEETQRILQKDCETYKKVLAETEGILQRLQSSVEEEESRWKVKLEQSQEDVRELRREKQHLESELERAENESATYVTEVRELKDLLTELQTRLDGSYTEAFRQNEELAVLKTQLSETLSRLETEEKERQRVAGDLYKAQQSLEQIQSELTKLIENTALIENTSLSSQREEIDKKERITAGLNQTVREMQQLLNAISQQLAKGQQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKQ9</accession>
    <name>A0A3B5LKQ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD repeat domain 63</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKQ9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008464"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008570.1">
        <property type="protein sequence ID" value="ENSXCOP00000008464.1"/>
        <property type="gene ID" value="ENSXCOG00000006491.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156924"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036156">
        <property type="term" value="C:inner dynein arm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036159">
        <property type="term" value="P:inner dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF5">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="338" mass="39073" checksum="72F58EFC57F9B830" modified="2018-12-05" version="1">MAPKARKRSPNLKNYSEFSPPGYPEDIFPLVLTSATQELFNCRIDEDVTEKSPFKLLKKDDIIDDMKRRAAVSDFSPVKQTVLAYPEEEMLLVFDSNLTYGQFFYLVLTPESKDRILKVSMQFFLCSLLSQNMFSLPFYNKNISYLSLKLRVDRAVQSKVCFSDRDCAESKDSHLECTSYEDSKFKIKKLLTDCGVQAVPMIQTNSCQTMRFWPLSRKQIERQFSTEEKETILKEQSLMNFCNAQIPRMLFALQQEEIINVFLDDWKALATGLEACDWSGQVSDTLVLHQVFTDQKFAKSKTISCLNWHPTIHGKVQHFKAIKLLSSHQEQKSNAKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKR2</accession>
    <name>A0A3B5LKR2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SH2B adapter family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKR2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011260"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011391.1">
        <property type="protein sequence ID" value="ENSXCOP00000011260.1"/>
        <property type="gene ID" value="ENSXCOG00000008507.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183191"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005068">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000008">
        <property type="entry name" value="SH2B adapter protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030523">
        <property type="entry name" value="SH2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10872">
        <property type="entry name" value="SH2B ADAPTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10872:SF1">
        <property type="entry name" value="SH2B ADAPTER PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="6">
        <location>
            <begin position="80"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="213"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="213"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="284"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="307" mass="34074" checksum="A653306695DBCD4C" modified="2018-12-05" version="1">PKLTTHCSDIQEVRRCTRLEMPDNLNTFVLKGSLIFETDNDQQVSSWTTELKECISNPVTFALPEQVIQKADDFLVSFPWFHGPISRVKAAHLVQSSGPDGHGVFLVRQSETRRGDFVLTFNYQGKAKHLRLSLNEWGQCRVQHLRFPSVMDMLSHFRLYPIPLECGAAGAVTLSSFVLAGSTTPSQQLSGALLVPFSLHRWSSEPSLARCSLARSSSQPPAFSPSPTSTTSTSSPGSFSQFGRAVPTTEPAPSAPAHLRRSESVGRRPLLRNPNPNTPIIPQRDSDYELESDRGRKRAIDNQYMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LN22</accession>
    <name>A0A3B5LN22_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ankyrin repeat and EF-hand domain containing 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LN22"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012387"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012536.1">
        <property type="protein sequence ID" value="ENSXCOP00000012387.1"/>
        <property type="gene ID" value="ENSXCOG00000009350.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156852"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052801">
        <property type="entry name" value="Ankyrin-EF-hand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24127">
        <property type="entry name" value="ANKYRIN REPEAT AND EF-HAND DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24127:SF1">
        <property type="entry name" value="ANKYRIN REPEAT AND EF-HAND DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="48"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="81"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="185"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="218"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="251"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="526"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="562"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="592"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="647"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="658"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="669" mass="74028" checksum="CC960FF5B272669C" modified="2018-12-05" version="1">MLNFQAGRLQVLQIYRLLQWIHKGEKEEIEKMVKLGVKNLINLTEPQEGLGVLHLAVSANNSDLVEFLLSLGAHPDVQDKMGRTPAMLAAELGNQEILSLLFQKQPDMTLVDMQGKGVLFYCSYPTKRHAHCLELALQNGADPNNISRSGVHVLSMMCEKANECTPLCLTLLDAGADPNGANETTGVTALMEAARTGSLPVVRHILRNRGNPNTLDKNGFNAVHYAALSGCFEVIMVLSGFAADMSVVNTESCTPLHYAAAAGTAPSCKFLAQRGCNPKVKNKEGLLARQIAKESGLKAAVKELKKAERAFGKSKKSTGGSYMSDLWALTLHDWSNEYQAELVEAFRDQFGFADLDTFKSVLENLRAPVEPDNLNLVFSAHNKKGEGLINVNDFIKGTKYIKKVFRLSVYTTQKKKKEKGPKMKKKKKGPFVLPIPICTLVPELKPRRPDGGPPHFMIEKYHNLTDVRRFDSEHPPLHPIVNDSWWYIEKPNKVYINMNYCVRNGDLESLNLAFDDGVPVDTQDEFYKTPLMEACISGNYELVQYLLEKGADVSICDQFFWTALHHAANRGYLEIVELLVNAGADVNAKALNDGTPLMRAIESSSFSCVEFLINAGAKVNSETKKGVTCLDVARHFGETGIESLVQEKVNALPDPKKSKPKPTKKKVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQ36</accession>
    <name>A0A3B5LQ36_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Membrane protein BRI3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Brain protein I3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Interacts with BRI3BP. Interacts with MGAT1 and IFITM3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BRI3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQ36"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011663.1">
        <property type="protein sequence ID" value="ENSXCOP00000011531.1"/>
        <property type="gene ID" value="ENSXCOG00000008707.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048486"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019317">
        <property type="entry name" value="BRI3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13551">
        <property type="entry name" value="BRAIN PROTEIN I3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13551:SF1">
        <property type="entry name" value="MEMBRANE PROTEIN BRI3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10164">
        <property type="entry name" value="BRI3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0458">Lysosome</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="129" mass="13778" checksum="AF25002301728330" modified="2018-12-05" version="1">MVDNKPLLQDRPPAYNAVPGAHEYGPPPQQPHGYGAIPAAAPPPYHYPDGYPPAQMGPAVSQQPYTSTYAIIQPSVVVVGGCPACRVGVLEDDFTCLGILCAIFFFPLGILFCFALRQRRCPNCGATFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQK3</accession>
    <name>A0A3B5LQK3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Stum, mechanosensory transduction mediator homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQK3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010177.1">
        <property type="protein sequence ID" value="ENSXCOP00000010059.1"/>
        <property type="gene ID" value="ENSXCOG00000007635.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008003"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026673">
        <property type="entry name" value="SPEC3/Stum"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21676">
        <property type="entry name" value="PROTEIN STUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21676:SF1">
        <property type="entry name" value="PROTEIN STUM HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15795">
        <property type="entry name" value="Spec3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="13795" checksum="C6BEAE3C3DBE8C25" modified="2018-12-05" version="1">SRNVHKRTKTMAGAPMAGGGVVVEVREKKGTLRAAIPTMPFPVAVICLFLNTFIPGLGTFVSAFTVLCGARSELISERGVCCVFWLNVAAALIQILTAVIMVGWIMSIFWGMDMVILAKQSKPPQLQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQZ2</accession>
    <name>A0A3B5LQZ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ribonuclease H</fullName>
            <ecNumber evidence="2">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) pol subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQZ2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011826"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011962.1">
        <property type="protein sequence ID" value="ENSXCOP00000011826.1"/>
        <property type="gene ID" value="ENSXCOG00000008944.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163417"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.10.10">
        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001995">
        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018061">
        <property type="entry name" value="Retropepsins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051320">
        <property type="entry name" value="Viral_Replic_Matur_Polypro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33064">
        <property type="entry name" value="POL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33064:SF37">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00077">
        <property type="entry name" value="RVP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50175">
        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A2" evidence="5">
        <location>
            <begin position="8"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="6">
        <location>
            <begin position="179"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="417"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="429"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="443" mass="48837" checksum="A5A19973D1610C95" modified="2025-10-08" version="2">MTVHGQPVTFLCDSGACKTCVKDSVGLKSSNTPIFVKSANGQTSQEWLSHFIDICDPQTGKRLHAPVVISPSCPINLLGRDLMGKLGIAIFPTKGGMKAVRVENVMATVTEKPIVLFDTEPKTLPASLWSTGPTDVGLITIVEPVTITPKSQCRPRIKQYPLKPDAQEGIKPVVQDLLDAGIIRLCPDSPCNTPIFPVQKADGKNWRMIQDLRAVNAAVQTRAPNVPDPHTLLNSLDPESKFFTVIDLSNAFFSIPVHPDSQYWFAFTFEGKKYTYTRLPQGFADSPTIFTQAVMTCLESFTPQNGSQILVYVDDLLIASKSKAACKADSLQLLHHLASTGNKVSKSKLQWVQQQVRYLGHQISENGKEIHDELEKRDERRTSAALPRDSAKQKRKPQAGRRAVQIEVLIWLQARREANRQESPPAGKPSSTPFANSLHVTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LR74</accession>
    <name>A0A3B5LR74_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LR74"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013459.1">
        <property type="protein sequence ID" value="ENSXCOP00000013295.1"/>
        <property type="gene ID" value="ENSXCOG00000010076.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154842"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038062">
        <property type="term" value="F:protein tyrosine kinase collagen receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010976">
        <property type="term" value="P:positive regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000007">
        <property type="entry name" value="Discoidin domain receptor tyrosine kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000053">
        <property type="entry name" value="Epithelial discoidin domain-containing receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000082">
        <property type="entry name" value="Epithelial discoidin domain-containing receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048525">
        <property type="entry name" value="DDR1-2_DS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF520">
        <property type="entry name" value="RECEPTOR PROTEIN-TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21114">
        <property type="entry name" value="DDR1-2_DS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017366643" description="receptor protein-tyrosine kinase" evidence="19">
        <location>
            <begin position="24"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="388"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="21">
        <location>
            <begin position="26"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="544"/>
            <end position="838"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="842" mass="95196" checksum="3A48AB7CFC1A8D9A" modified="2018-12-05" version="1">CLKNPRMYFVYFTAAPLCLLVFSETCRYPLGMTGGQIQDEDISASSQWSESTAARFGRLDFDTGDGDGAWCPDIMSEPGGFKEYLQVDLRSLHFITLVGTQGRHADGMGNEFAQRYRIKYSRDGGSWMGWHDRMGNQVVEGNKNTYDVVLKDLEPPIVARFVRFMPVTDPSSIVCMRVELYGCEWLDGLVSYSIPDGHQMIYRGLDVFFNDSVYDGATAQRLTKGLGQLTDGAWGLDDFLHSHVPGAWPGYDYVGWSNKTFPKGYVEVVFDFDHIRNFTSMKVHCSNMFSRGVRLFRQATCFFRSESEWEPDPMTFRPSAEPLSQSGRFVTVPLGDRTASSIKCRFHFGDQWLLFSEVAFQSGKTIRALPFTTREDPTHKVDDSNTKILIGCLVAIIAILLTVIVIILWRQVWQKMLEKASRRWLDDELTARLAAQTQAFSFLRSSQSSEDGSGSGSISTYERIYPACADYQEPSRLIRKLPEFAECSGTSSRAGVGSDGAPHYAEADIISLQESSDSGSITAVNMNLFAGSDLTLREFPKEKLTFKEKLGEGQFGEVHLCEAEDMQDFLGEDTSTEGSSESPLLVAVKMLREDANKNARNDFLKEIRIMSRLRDPNIVRLLAVCVDTDPLCMITEYMENGDLNQFLCSLRLKTLTGGEADQQEGSDEVRYSVKLLGMAVQVVSGMKYLSSLNFVHRDLATRNCLVGKNDTIKIADFGMSRNLYRGDYFRIQGRAVLPIRWMSWESILLGKFTMASDVWAFGVTLWEILTLCKEQPYSQLSDEQVIENTGEFFRNQGKQVYLPKPQCCPDRIYVNLMLSCWRRNAKERPSFQEIHAQLTETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSH4</accession>
    <name>A0A3B5LSH4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sarcalumenin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Sarcoplasmic reticulum lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Sarcoplasmic reticulum membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSH4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013770"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013939.1">
        <property type="protein sequence ID" value="ENSXCOP00000013770.1"/>
        <property type="gene ID" value="ENSXCOG00000010424.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157834"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033018">
        <property type="term" value="C:sarcoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033017">
        <property type="term" value="C:sarcoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09913">
        <property type="entry name" value="EHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000635">
        <property type="entry name" value="Sarcalumenin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031692">
        <property type="entry name" value="EHD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051943">
        <property type="entry name" value="TRAFAC_Dynamin-like_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43681:SF1">
        <property type="entry name" value="SARCALUMENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43681">
        <property type="entry name" value="TRANSMEMBRANE GTPASE FZO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16880">
        <property type="entry name" value="EHD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="10">
        <location>
            <begin position="53"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="436" mass="49986" checksum="1D1935E9058902F0" modified="2018-12-05" version="1">PSSDTGPLCSVLLSRFPAAIQKLRKIYHSSIKPMEQAYKYNELRQHEISDGEITSKPMVLFLGPWSVGKSSMINYLLGLHDSPYQLYTGAEPTTSEFTVIMHGEKIRSVEGIVMAADSSRSFSPLEKFGQNFLEKLIGIEMPHKLLERVTFVDTPGIIENRKQQERGYPFNDVCQWFIDRADLIFVVFDPTKLDVGLELEMLFRQLKGRESQIRIILNKADNLATQDLMRVYGALFWSLAPLINVTEPPRVYVSSFWPYDYAPDTSRELFKREEISLLEDLNQVIENRMENKIAFIRQHAIRVRIHALLVDRYVQTFKEKMTFFSDPELVFQEIVDEPDKFYIFKSILAKTNVSKFDLPNRDAYRDFFGINPIANFKSLASQCSYIGGCLLDKIEKAITNELPALLSSINSGKQPGLSSCEVTGCGEKPKNRYRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTQ3</accession>
    <name>A0A3B5LTQ3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha-2-macroglobulin bait region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTQ3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014502"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014687.1">
        <property type="protein sequence ID" value="ENSXCOP00000014502.1"/>
        <property type="gene ID" value="ENSXCOG00000010997.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155926"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF136">
        <property type="entry name" value="CD109 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0882">Thioester bond</keyword>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="3">
        <location>
            <begin position="239"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1013" mass="113175" checksum="C38F693474C20E1D" modified="2018-12-05" version="1">LLFTNTTSLTYNLWTVSLFIQTDRSSYKPGDTVNVRVVTFQLDNKPYKGRVDISLLDPSGKPVESWNSTGNLGIALKEFSLSLMSRPGQWMIAATVNYRPTPFRSMGQPSFSSAEINFRLCTPSYQLATAMFYTLLSSLKVAPQVCFILNNSFNISALYHNIFVSVAVAPGFTVNKTVEVHVLRNKFQLMFLNFPSTLKPSLNFSTNRSSLMNSTTTSLTFSVPENGNVLIRFKLQDQVEMLLIQVSIMSHSSQKYSRSSRKSTILSFFASNMSGFSTISSKGQVVAAGTKNFSSSVTLTPELSWYPEACVTVYYNHSDGEITSDTAYISIHQMFTCSFQVTLNWSSERARPGEQVSLSVAGSESRFQVGITVTAMQNDDLQSGSVDLDYRIEQVVEHFGLEKKSKFNFYDDQRKILFEKPRRWTWKHGKITVPNGVTLLQYKPVLLPNLLLWEEVILEVNVINHLEQEIEFVLMDQRGASIINAQKITLRSHESASALFPIRPLALGEMEISVDAVSAEASDGLVEKIWVKPEGVEQTFSHTLFLEMTPEKQDSSRSVTFSFPPNLVPGSQRAHVALVGDILALSIENLDSLVQLPTGSGEQNMVHFAPSVFVLQYLEKSGQDDEGIRNKALRYMMQGYEKQLSYQRDDGSFSVIIWLTALVLRYFLQAQPYMQIDQTVLTKATAWLLKHQDSQGAFTEVGKLIHTEMREGQDDSPVALTAYVLLEMYANNVSLSLRYLESKVSSGIVSNYTLCLVAYALALGKSVVLETVMAELTRRADYRGESECLRSNNQQQHSTQIEMASYVLLVQFLLGSFIESIPLMKWLSMQRSYLGGFGTTQDTVVALQVLSYYAAFSGANAINLVVNISSPMSSTASLFLINSTNYRVYQSREINAAEDLPLNISMEGIGFAIFQVNIFYNTKSTQDIELTTDKDAFILNVDLTHNDSNHIMLSVCTRLKDNQEIAHTGMAILEVGMLSGFSLFSGAAVQADFIRRVDIGPERVSLYLNSVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVB4</accession>
    <name>A0A3B5LVB4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Si:dkey-183n20.15</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVB4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011910.1">
        <property type="protein sequence ID" value="ENSXCOP00000011774.1"/>
        <property type="gene ID" value="ENSXCOG00000008898.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017123"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16553">
        <property type="entry name" value="RING-HC_RNF170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038896">
        <property type="entry name" value="RNF170"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22894">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22894:SF2">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="45"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="242" mass="26551" checksum="B399059283BD559E" modified="2018-12-05" version="1">MPQLSLNLFFLTSCKLKLDLESNLDRRFNESTQSRPSMSSRDSHCPVCLQTATFPVQTNCGHLFCANCLLTYWRHGSWLDAVSCPLCRQKVSVLCNLFKESRSDQQSKKVLGEITDYNKRYSGAPRRVTDYLCDTPLLLQLLARNCLGTMGGLVWLFLFRVALCCVGTVVSISSSPSVDSAPSSTPLETDPSLCGLLGVLDDLVVVILLLLCVINVNQQIAPDGGRHSPNRTTSQGVMGSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LW30</accession>
    <name>A0A3B5LW30_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LW30"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012044"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012182.1">
        <property type="protein sequence ID" value="ENSXCOP00000012044.1"/>
        <property type="gene ID" value="ENSXCOG00000009113.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000178424"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="130" mass="15461" checksum="4F5F13972F03DE4B" modified="2018-12-05" version="1">KFIKLQEEQAQLQRKVEKLRRKLEQLNSNSADNHQAEQNVLNNINEFYKDKIRSLNLDLDKEREKIYKLRWKHDAIVKRNEAEDQEEAHRRRLTAINTDIDSILRDIANLEDIRNSIPAGCYNTPPIEEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LW57</accession>
    <name>A0A3B5LW57_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MORN repeat-containing protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Plays a role in promoting axonal degeneration following neuronal injury by toxic insult or trauma.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with MYO3A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Filopodium tip</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Stereocilium</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027863347.1">
        <property type="nucleotide sequence ID" value="XM_028007546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LW57"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016198"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016404.1">
        <property type="protein sequence ID" value="ENSXCOP00000016198.1"/>
        <property type="gene ID" value="ENSXCOG00000012245.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114138333"/>
    <dbReference type="KEGG" id="xco:114138333"/>
    <dbReference type="CTD" id="118812"/>
    <dbReference type="GeneTree" id="ENSGT00730000111173"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032433">
        <property type="term" value="C:filopodium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032420">
        <property type="term" value="C:stereocilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048678">
        <property type="term" value="P:response to axon injury"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052315">
        <property type="entry name" value="MORN4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46614">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46614:SF1">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="146" mass="16119" checksum="3F17E289FA5D6C40" modified="2018-12-05" version="1">MTLTRGSFTYASGEEYHGEWKEGRRHGLGQLKFQDGTCYTGQFENGLFHGSGVLLFTDGSRYEGEFAHGKFQGSGIFSRFDGMKFEGEFKDGRVEGYGLLTFPDGTHGAPRNEGLFQNHKLQKREKCPGVVQRAQASASSAHSLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LY95</accession>
    <name>A0A3B5LY95_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Potassium channel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Cs(+)(in) = Cs(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78555"/>
            <dbReference type="ChEBI" id="CHEBI:49547"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Rb(+)(in) = Rb(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78547"/>
            <dbReference type="ChEBI" id="CHEBI:49847"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LY95"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016127"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016331.1">
        <property type="protein sequence ID" value="ENSXCOP00000016127.1"/>
        <property type="gene ID" value="ENSXCOG00000012186.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156560"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000043">
        <property type="entry name" value="Potassium channel subfamily K member 10 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003976">
        <property type="entry name" value="2pore_dom_K_chnl_TREK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF21">
        <property type="entry name" value="POTASSIUM CHANNEL SUBFAMILY K MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01499">
        <property type="entry name" value="TREKCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="16">
        <location>
            <begin position="144"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="16">
        <location>
            <begin position="239"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="437" mass="48476" checksum="A9C703BE7E074C88" modified="2018-12-05" version="1">MVQNEGEHVKNLNNNPVFSAVAAPDLLDPKSATHNTKPRLSFSTKPITLTPREESEVVATVMKWKTVTAIFLLVVLYLVIGAAVFRWLEQPHESAQRLAILSQKLDFLSQHSCVNQSQLEELVQQVVSAVRSGVNPAGTYTNHNSLWDLSSAFFFAGTVITTIGFGNISPHTEGGRIFCIIYALLGIPLFGFLLAGVGDQLGTIFGKGIARVEKMFVHWAISQTKIRVISTLLFVLFGCLLLVALPAAIFKHIEGWSALESLYFVVITLTTIGFGDFVAGGSDIEYLDYYKPLVWFWILVGLAYFAAILSMIGDWLRAISRRTKEEVGEIRAHAAEWTANVSAEFKETRRRVSVEIYDKFQRAASIKRKLSTELGFSSAPELTLPRRATSINFNDERERNDKEAGLKGLTTPLTRNGSLVNGLDLERGDVSIIEHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZT4</accession>
    <name>A0A3B5LZT4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA (carboxymethyluridine(34)-5-O)-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.229</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Alkylated DNA repair protein alkB homolog 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">S-adenosyl-L-methionine-dependent tRNA methyltransferase ALKBH8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-(carboxymethyl)uridine(34) in tRNA + S-adenosyl-L-methionine = 5-(2-methoxy-2-oxoethyl)uridine(34) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:10408"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74851"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.1.1.229"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alkB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.229" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZT4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013259"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013423.1">
        <property type="protein sequence ID" value="ENSXCOP00000013259.1"/>
        <property type="gene ID" value="ENSXCOG00000010055.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158563"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106335">
        <property type="term" value="F:tRNA (5-carboxymethyluridine(34)-5-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051422">
        <property type="entry name" value="AlkB_tRNA_MeTrf/Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069:SF21">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="15">
        <location>
            <begin position="187"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="491"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="575"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="491"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="580"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="650" mass="72544" checksum="B1FD87E4BA2641FB" modified="2025-10-08" version="2">MRRGKEVKKVLRKQLKASHTLLKHEGISTASQPTKNLVVANGGLGNGVSREDLSAAMKQFGDVESLFMPPQKPYAFVTYRYHRSKNLLFIIKAICSKEVLVRFPEELALVEDFVSPEEEILLLAAVDWSTSNQAQKALKHRRVKHYGFEFRYDNNNVDKDKPLPAGLPEECLPVLHRCLQDGHINVLPDQLTVNQYETGQGIPPHIDTHSAFEDTILSLSLGAKTVMEFRHPDGRVVPVVLPERSLLVMKGESRYLWTHGITPRKFDLVPACETQSADHSEPNLVSHGGLTLSKRGTRTSFTFRKIRHKPCCCAFPSACDSQGGSSPCPPSQPSLPSCHADATRLEEEYVHRVYDAIASHFSSTRHTPWPRVCHFLSALQPGSVLADVGCGNGKYLGVNPQLITVGCDRSSSLVQICAERGFNAFVSDALSVPLRTDSCDACISIAVIHHFSTQERRLAAVRELVRLLKPGGQALIYVWAYEQEYNKQKSKYLKDHNKPQNGEKSKDKPEEGQEPHEEPSVLTTSHLDNNYIGGLKDDSKMTESKLSVHTNRTAFNSQDLLVPWHLKGRKTRGEEEFSSGLHTTNCPDSKLSPSVDTSQVPGGSSVPVFHRYYHVFQQGELEQLCIEVTGVEVQTSYHDQGNWCVILQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0R1</accession>
    <name>A0A3B5M0R1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHF complex subunit HOOK-interacting protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FHIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0R1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015201"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015392.1">
        <property type="protein sequence ID" value="ENSXCOP00000015201.1"/>
        <property type="gene ID" value="ENSXCOG00000011515.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182936"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019384">
        <property type="entry name" value="FHIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045669">
        <property type="entry name" value="FHIP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045668">
        <property type="entry name" value="FHIP_KELAA_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21705:SF9">
        <property type="entry name" value="FHF COMPLEX SUBUNIT HOOK-INTERACTING PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21705">
        <property type="entry name" value="RAI16 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19314">
        <property type="entry name" value="DUF5917"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19311">
        <property type="entry name" value="KELAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHF complex subunit HOOK-interacting protein C-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="134" mass="15058" checksum="66F61BD86BC9CBBE" modified="2018-12-05" version="1">MSRCQPYEVNLQLTAVLSRLCAFSHPLLDEYLLDPFVHLSAGSRSLFAVLVRVVGELMERIQQVVDLPQRLLDTRRRLLGLHLDHPALLQGVIVLEEFCKELAAIAFVKMPLDRNHDRFCSGPGSVGPEPAERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M299</accession>
    <name>A0A3B5M299_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PDZ domain containing 7a</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M299"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017527"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017749.1">
        <property type="protein sequence ID" value="ENSXCOP00000017527.1"/>
        <property type="gene ID" value="ENSXCOG00000013212.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183002"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002142">
        <property type="term" value="C:stereocilia ankle link complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:stereocilium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:auditory receptor cell stereocilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10833">
        <property type="entry name" value="PDZ1_PDZD7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10834">
        <property type="entry name" value="PDZ2_PDZD7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06751">
        <property type="entry name" value="PDZ3_PDZD7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051844">
        <property type="entry name" value="USH2_Complex_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23116">
        <property type="entry name" value="PDZ DOMAIN CONTAINING WHIRLIN AND HARMONIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23116:SF29">
        <property type="entry name" value="PDZ DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="73"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="197"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="867"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="302"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="357"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="408"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="501"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="715"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="14"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="304"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="377"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="420"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="439"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="449"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="472"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="715"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="766"/>
            <end position="776"/>
        </location>
    </feature>
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        <location>
            <begin position="794"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="842"/>
            <end position="851"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="964" mass="106418" checksum="952233A6F5BD143E" modified="2025-10-08" version="2">MQHNTDTAGKTMTNGGGHPHFSNGGRGGGSQSTTRYMLKKQQRHRRRSSSPMGRVILINAPVDGGDDSEDIHTVTVNKSPDGRLGFSVRGGSEHGLGIFVSKVEDDSTAAQAGLTVGDKLVEVNGVSLESITMSSAVKVLTGNNRLRMVVRRVGKIPGIRYSKEKTTWVDLIHRRMVVEENGRTPSEASSDSALRRIVHLFTTSDDYCLGFNIRGGKEFGLGIYVSKMDPGGLAEQHGIKMGDQILAANGVSFEDITHSNAVEVLKSHTHVMLTIREVGRYPAYKEMVAEYGWLDKLANGGPALSSQGSDSNSSVSSLSSSTPVSSLSGLSQVLFPPVFRSEMVDVAICTEDRSRRQSIAERTADSAIQTDPPAADFMDHSPNRLDPDRLVGTETSRTVGETMLLKDTVIRGKVRTPSAGERDAEKQSPKTEALMALSKPRKPIRRSQSHITVSGETSTLPEKNFTKTFSASDKKRQQKQKDSADGKTNLQRSKTFVNLFFKKDHKDKSRSRSPSYHPNEKERSRPFQLFTSPRESRAGSPLPHHETLQHVEDMSKKLLSPDEVAAVMRNCRRFLSDNVIEDLVRPLLAILDRPEKLLLLREIRMLIPTSELGRFDSLVMPFELEAYDILKSRSIRSPALRSPRSGTPRRHLITPIPDYRGGFHLQPVHDTHQERQLIEDFERLRLTSQQTGHLPPSRAFIPLLDVPVDNYASSIARSRTPSPSHSFLLSGSPHNTQPGRQPHRSPNRSENGTPRYDQVSLLSVSDRGDAAPERGRSPVRSSHGRGRREGSPDSIYSRQSRQDGYTEVSVRVPSQRRGRTPLADVFDPQRERSPSSRRGSGLQVNGHFQNGQERRGTLPAEEYEIMTLTISKAKQSLGISISGGIESKIQPVIKIEKIFPGGAASTNEALKAGYELLSVDGESLQGVTHQHAVDVIRRAFSNKAKDPMVFVVKVPKVPLVPPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M459</accession>
    <name>A0A3B5M459_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M459"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019175.1">
        <property type="protein sequence ID" value="ENSXCOP00000018938.1"/>
        <property type="gene ID" value="ENSXCOG00000014244.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154848"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042074">
        <property type="term" value="P:cell migration involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007398">
        <property type="term" value="P:ectoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007498">
        <property type="term" value="P:mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001841">
        <property type="term" value="P:neural tube formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000011">
        <property type="entry name" value="Cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000095">
        <property type="entry name" value="Cadherin 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000022">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF319">
        <property type="entry name" value="CADHERIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Cadherin" evidence="11">
        <location>
            <begin position="104"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="11">
        <location>
            <begin position="207"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="11">
        <location>
            <begin position="346"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="11">
        <location>
            <begin position="447"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="595" mass="65452" checksum="BD5448AB1FFE2DC0" modified="2018-12-05" version="1">SCEPGFESELFILKVSRKNLKPGTRLSKGISIPSISINAYWGQRGNLLPGREFNPEMVSLSLPPSPPSLHHSCLCFSSGIPILDFPKSNPGLKRRKRGWVIPPINVPEHSRGPFPLFLVVIRSDEDKVKTIYYSITGPGADQPPVGLFTMNRDTGHLHVTQELDREKVDKYTLQAHSVAEGGDGKAEEPMEIIIKVIDQNDNKPVFSQSVYVGEVPEASQEGFQVIQVVATDADEPNTDNSDIRYHVMSQKPEEPSPNMFTINPTTGVIRVNEAGLDREKIPQYTLEVQAADNKGEGLTGFTKVIIKVTDLNDHTPIFIEPFVSIRHDLITCEYFIQISLSQLICAADEKHVSKSEDLAVNSTVVRYTATDPDTGRKQKVMYEIISDPAGWFDIDKETGEIKVRNQMDRESPLVKEDKYSALIDQIPATATGTLVLHLQDVNDSPPTIEERKFTVCSKIPVPQLLTVTDRDGPGFTSPYNVSLVGSAKANWTARMISTRTGIILTLNRELEIGDYTVAMSIADSQGLSQVSTVTARVCDCTGEDVSCREKDVGGSNSPVILGILKAIVVLLISGSDSQDPEYEYLAEGEPLSETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M467</accession>
    <name>A0A3B5M467_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M467"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018252"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018483.1">
        <property type="protein sequence ID" value="ENSXCOP00000018252.1"/>
        <property type="gene ID" value="ENSXCOG00000013747.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000178312"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="126" mass="14262" checksum="414052C31FBD1624" modified="2018-12-05" version="1">ISKPRPPLGKSNHNLVFPCSEYKPLSEGALTVVENTEGGRGVDEEEEVTVDNDDDDDGVFEVVQPNEQWQTLKPGEAVPAGSHVRLNLQTGEREVRLGEEQLKYWTQQHRWICKRVIVWSLPLTIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M593</accession>
    <name>A0A3B5M593_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M593"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015339.1">
        <property type="protein sequence ID" value="ENSXCOP00000015149.1"/>
        <property type="gene ID" value="ENSXCOG00000011480.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155932"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000780">
        <property type="entry name" value="myc-associated zinc finger protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000095">
        <property type="entry name" value="Vascular endothelial zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF66">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="201"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="387" mass="42257" checksum="CF3E48AB8A9341C8" modified="2018-12-05" version="1">MDTSWSNFLFQTTPTQTQAEAPLQAELLPDLTGSAQSPPAEHIVTPPSTVDTAALSEEPLPVKPLPKPPRPAHICSTCNKEFKNSYNLRRHQSVHTGIKMKDRTARDKEDGAKAGRLEKTTIPLSLLQLTLPPQPLPPAGAPDAQPLSVSVAPATVTVASPPQPIQAAIVVVGSMEQVGQPVSKNPSPSPVPNPNQVRKNHACEACGKAFRDVYHLNRHRLSHSDEKPYSCPVCQQRFKRKDRMSYHVRSHQGGVEKPYICPHCSKNLSASDPPLRLSQTEPGSEPGPGAELLLAMEVYFYSHLHGNQSAAISRTSPGSAVLTHLCSIYQCLVLMKWTGLLLRTPSLPLLEQEKLRSEVLPSRAGPRNLWFGVNSPEAERAAIFDLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5R9</accession>
    <name>A0A3B5M5R9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">OCEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ELL/occludin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M5R9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018787"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019023.1">
        <property type="protein sequence ID" value="ENSXCOP00000018787.1"/>
        <property type="gene ID" value="ENSXCOG00000014144.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155914"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2670">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042065">
        <property type="entry name" value="E3_ELL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031176">
        <property type="entry name" value="ELL/occludin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019464">
        <property type="entry name" value="ELL_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010844">
        <property type="entry name" value="Occludin_ELL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288">
        <property type="entry name" value="OCCLUDIN AND RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288:SF9">
        <property type="entry name" value="RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10390">
        <property type="entry name" value="ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07303">
        <property type="entry name" value="Occludin_ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144292">
        <property type="entry name" value="occludin/ELL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51980">
        <property type="entry name" value="OCEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="OCEL" evidence="8">
        <location>
            <begin position="312"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="169"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="263"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="208"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="263"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="430" mass="49438" checksum="9A96D5E9463C873C" modified="2018-12-05" version="1">MAQAEEETRSRGAIVIKNGARYQGKKNGKALACLQRVCDYTTRNPAQRKGPCPVSRKSVGDIRERPLRERVMHLLALRPYKRPELILRLQKDGIADGDKDVLDTDGGGLFVLQVGQLSNRDSTFVLKDGLYKELQKDWPGYTSGDQQLLKRILVRRLFQPQQNLLTVPEAQVSPLRETPNSSPAHRPKHSLLDEYADPLASKKPRISHLTSKSTSENFRLRSSKQEAQKDAEATAEDGQNNSLNPKRLFDSLSAVCQQEAEVAKRLEQTDAPQEEPRDPEEQQKPKSDHPSLPLMVPNLSRHTVKKKKSKHKDQVRKQIPALSHNQRQSYKLDFNKEYSEYRDLHACIDSVTQQFMDLDTQLKQLHHESHKYKTVRNKILQEYRKIKKSNPNYNQDKVRCEYLHNKLAHIKKLISDFDQQQITVDHSGPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6X7</accession>
    <name>A0A3B5M6X7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Neuropilin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the neuropilin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6X7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019257"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019495.1">
        <property type="protein sequence ID" value="ENSXCOP00000019257.1"/>
        <property type="gene ID" value="ENSXCOG00000014480.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155270"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017154">
        <property type="term" value="F:semaphorin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005021">
        <property type="term" value="F:vascular endothelial growth factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd06263">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000002">
        <property type="entry name" value="Coagulation factor VIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000013">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000003">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000010">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000998">
        <property type="entry name" value="MAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014648">
        <property type="entry name" value="Neuropilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022579">
        <property type="entry name" value="Neuropilin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050633">
        <property type="entry name" value="Neuropilin_MCO_CoagFactor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806:SF2">
        <property type="entry name" value="NEUROPILIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11980">
        <property type="entry name" value="DUF3481"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00629">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036960">
        <property type="entry name" value="Neuropilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00020">
        <property type="entry name" value="MAMDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00137">
        <property type="entry name" value="MAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00740">
        <property type="entry name" value="MAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50060">
        <property type="entry name" value="MAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 17" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9 17" id="KW-0221">Differentiation</keyword>
    <keyword evidence="14 19 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="13 17 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 17" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="15 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="812"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="22">
        <location>
            <begin position="24"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="22">
        <location>
            <begin position="144"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="23">
        <location>
            <begin position="272"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="23">
        <location>
            <begin position="429"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="MAM" evidence="24">
        <location>
            <begin position="600"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="19 20">
        <location>
            <begin position="24"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="144"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="272"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="429"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR036960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR036960-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR036960-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="878" mass="98040" checksum="68C9D0B71A51DEED" modified="2018-12-05" version="1">MCSKYHLYNMGWMVRLFYFTSEPCGGNLDATYAGYITTPGYPQEYPPHQNCHWVITAPEPSQRIVLNFNPHFEMEKLDCRYDYVEIHDGNSESADLLGKHCSNIAPAPIISSGPSLHIKFVSDYAQQGAGFSLRYEIHKTGSDCSRNFTSPSGLIESPGFPDKYPHNLECSFIIIVPPSMDVTITFLIFDLENDPLPGSDGDCKYDWLEVWDGLPAVGPLIGRYCGTKVPPEIQSSTGILSLAFHTDMAVAKDGFSARYNMTHKEVSETFHCSNALGMESGKITDEQIMASSSFHDRNWLPRQARLNNEENGWTPAEDNNREYIQVDLHTLKVLTGIATQGAISKETKNIYYVSSFKLEVSTNGEDWMIYRHGKNHKVFHANTDATDVVLNRIPQPVLTRFVRIRPQTWKNGIALRFELYGCQITDAPCSELQGMLSGLLPDSQISASSMRDIHGSMGAARLVASRSGWFPNPAQPIAGEEWLQVDLGVPKTVRGIITQGARGLEGSTSADNRAFVRKYKLAHSLNGKDWSYIIDSKTGIAKIFEGNSHYDTPEVRRFDEIVAQHIRVFPERWSPAGIGMRMEVLGCDLPGKTTTPSLSAVCDFEKSLCGWSADPHSGVSWTLHGASGDFSGNYLHLDAGSHTQRKKARLLSPEVGPERGPLCLLFYYQLQGEAQGSLRVLLRDSDQEETLLWTLKGEHGPHWKEGRTILPQSPREYQVVFEGFFDHPTRGHIRIDNIHMSNSIELEQCAPSRFLSRVKVFFPKFLVFSPDFVFSGWPSSFSTPSSDMDPSSVTLVSEKDKDNAWLYTLDPILLTIIVMSSLGVLLGAVCAGLLLYCTCSYSGLSSRSSTTLENYNFELYDGIKHKVKINQQRCCSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M753</accession>
    <name>A0A3B5M753_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M753"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017181"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017401.1">
        <property type="protein sequence ID" value="ENSXCOP00000017181.1"/>
        <property type="gene ID" value="ENSXCOG00000012954.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157208"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001912">
        <property type="entry name" value="Hamlet, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000112">
        <property type="entry name" value="Mds1 and evi1 complex locus protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000126">
        <property type="entry name" value="Mds1 and evi1 complex locus protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000150">
        <property type="entry name" value="Mds1 and evi1 complex locus protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000159">
        <property type="entry name" value="Mds1 and evi1 complex locus protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000192">
        <property type="entry name" value="Mds1 and evi1 complex locus protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000929">
        <property type="entry name" value="Uncharacterized protein, isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF57">
        <property type="entry name" value="ZINC FINGER PROTEIN 154-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="125"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="182"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="739"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="767"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="796"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="316"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="529"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="858"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="371"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="419"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="576"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="609"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="858"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="894"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="965"/>
            <end position="978"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1009" mass="112631" checksum="971A2E9F015BF30D" modified="2018-12-05" version="1">MKAEEYFCDTMAPDIHEERQYRCEDCNQHFESRNELLDHQKEPCGMPPSAFLNPEPQDLRPLHMSHGLHECKECDQVFPDVQSLENHSQSHSEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCSKVFTDPSNLQRHIRSQHVGARAHACSDCGKTFATSSGLKQHKHIHSSVKPFMCEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHFTAGGLFAQGLPLPGTPGLDKSALTLGHSSAGLADYFGASRHHSGLTFPAAPAFPFSFPGLFPSGLYHRPQLIPATSAPIRQTAHAPVTGPGVELRKSPLLPPSPGALESQELLKALCKNGSSPGNDIHDSELPNQGSSVSTKQQNKQSDQSESSDVDDVSTPSGSDLESTTGSELESDMDSERERGVARENGKGPRRKATERDPQSPSLIGSSTAKGFPGQSLMQSSLDEHTAVTGAVNDSIKAIASIAEKYFGSAGLAGLQDKKVGSLPYPSMFPLPFFPTFSPPVYPLPDRDLRPTGVKGEPQSPGDDCKKAQSKSSESPFDLTTKRKEGKLSQFVPTKTDVANVTGQDQPLDLSLGSRGRGQIPCQEETRKNKSFEEAKRAVDVPKADTSLQHARPTPFFMDPIYRVEKRRMSDPFETLKDKYMRPTPGFLFHPQFRLPDQRTWMTAIESMAEKLETFGSLKPESGELLRSVPSMFDFRAPPSTLPETLLRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHIRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENGHLSGTAMSSPQSELDSSSAILDDKEDSYFNEIRNFISSASQNQTSPDPSEEGLNGGLLEEGKPLMPSHGSRDLEDEEGEDLDAEEEEAEEPRNSPQKTDVKVHPSSVGDDEMDFIEPNDLNLSCKTSPRRYSHEEEQSSFSALDHIHRFSDLRKMEESELNEDDGSFASPSLAKAVKQPLFRKSKSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8V4</accession>
    <name>A0A3B5M8V4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M8V4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019950"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020197.1">
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        <property type="term" value="P:negative regulation of neuroinflammatory response"/>
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        <property type="entry name" value="Immunoglobulins"/>
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        <property type="entry name" value="Ig_V-set"/>
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        <property type="entry name" value="OX2G-like"/>
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        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46841">
        <property type="entry name" value="OX-2 MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="49"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
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    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="147" mass="16842" checksum="C118CC65F1B84B54" modified="2018-12-05" version="1">MTSALYLFENFYYQNRSFLLQLLHIYNIQLDIVVLAAIDTQQSVLAAVGDQASLSCQLSKTKDVLQITWQKVLPLGEKNLATYTKKFGYRVSSGLEEKMDFQYESLQSCSMVIRKVTEQDEGCYRCLFNSYSEGALIGWTCLRLYGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M980</accession>
    <name>A0A3B5M980_9TELE</name>
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        <recommendedName>
            <fullName evidence="8">Golgin subfamily A member 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GOLGA7</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May be involved in protein transport from Golgi to cell surface. The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Golgi apparatus membrane</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ERF4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M980"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020092"/>
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        <property type="protein sequence ID" value="ENSXCOP00000020092.1"/>
        <property type="gene ID" value="ENSXCOG00000015063.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019383">
        <property type="entry name" value="Golgin_A_7/ERF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051371">
        <property type="entry name" value="Ras_palmitoyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13254">
        <property type="entry name" value="GOLGI AUTOANTIGEN, GOLGIN SUBFAMILY A, 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13254:SF1">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10256">
        <property type="entry name" value="Erf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0564">Palmitate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Golgin subfamily A member 7/ERF4" evidence="9">
        <location>
            <begin position="11"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="134" mass="15592" checksum="B6818C54A8C804B8" modified="2018-12-05" version="1">MRQQAAVAAKVFIQRDYSNGTMCQFQTKFPSELETKIDKQQFEETIRTLNNLYTEAEKLGSQSFVEGCLACLTAYAVFLCMETHYEKVLKRISKYIQEQNDKIYAPRGLLLTDPIERGLRVIEVTIYEDRSFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9E2</accession>
    <name>A0A3B5M9E2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Annexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="domain">
        <text evidence="6">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the annexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027861173.1">
        <property type="nucleotide sequence ID" value="XM_028005372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9E2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020409.1">
        <property type="protein sequence ID" value="ENSXCOP00000020162.1"/>
        <property type="gene ID" value="ENSXCOG00000014952.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114136938"/>
    <dbReference type="KEGG" id="xco:114136938"/>
    <dbReference type="CTD" id="312"/>
    <dbReference type="GeneTree" id="ENSGT00940000166789"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000002">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000003">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000018">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
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        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018252">
        <property type="entry name" value="Annexin_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF96">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00223">
        <property type="entry name" value="ANNEXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0041">Annexin</keyword>
    <keyword evidence="3 6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 6" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="316" mass="35464" checksum="413FEC016DA5C344" modified="2018-12-05" version="1">MGNCQATVFPYEDFDVVADIKAIRKACKGFGTDEQAIIDILANRSSAQRQEIKAAYFEKYDDELVDVLKSELSGNFENAILAMLDPPVVYAVKELRKAMKGPGTDEDVLVELLCTATNADIAMFKECYFQVHERDLEADIESDTSGDVRNLLMALLEGNRDESYDVDEDLAEQDATALFEAGEGCFGTDESTFSYVLANRNYLQLQATFKIYEQLSGTEILDAIDNETSGTLKKCYTALVRVAKNPQLYFARRLHKAMKGAGTDEDTLIRIIVCRSEFDLETIKDMYLEKYDVSLKDAIRDECGGDFKRLLLAICH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9F7</accession>
    <name>A0A3B5M9F7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Immunoglobulin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9F7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018140.1">
        <property type="protein sequence ID" value="ENSXCOP00000017916.1"/>
        <property type="gene ID" value="ENSXCOG00000013499.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005284"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin" evidence="3">
        <location>
            <begin position="5"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="472"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="525" mass="57387" checksum="1B75D95B600FB758" modified="2018-12-05" version="1">AGPPPERRTAFFGEDVHIEVSSWSDPEVVFRPRTNSSYEVPLLQAGRVTNQNKTELNSLGHLVLKDVQEEDEGLYIIRSGRNVSISKNLVLIVRDCVLEQVVKYGETYIIHLSQIKGPITLEFRVNQTVVPHTSEPPPVLLYNRSAVLGDDYVGRLSVSDRQVMLHSVRMTDEGSFTVLDRDGHTKTRSCLNVRDHQSFMHFAYGENLKMKLFLHYSNLNVVYRSKLDNQDRVIVNQGVLLAPLDPQLEGRLTVDGSELVMKKMQPADSGLFKVTDLAGFTVAHVYIDVEAYKMAPLAVAILSMLGLIAFLLLVCLLSCLYKIHKRNEKNKKLTLIAQQAGKKDGEAFRQVVHEAYSRFTEESLMQSVCDKPSETTEVTIKGLEVSKPGRYQTLPSDNFLEMSDSGVEFSHSGLPLDSDTEAVMTYASHKPLLNAASPPAVTDGVMMSDSLEAMAAMDGDLSAVRTPDSVLSASPASNPRSASAATPDGSLQGAASPATASRVTAGSDSAKTEGGAEGEEDGQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9Q7</accession>
    <name>A0A3B5M9Q7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C-Maf-inducing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9Q7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018016"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018243.1">
        <property type="protein sequence ID" value="ENSXCOP00000018016.1"/>
        <property type="gene ID" value="ENSXCOG00000013572.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165840"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000030">
        <property type="entry name" value="C-Maf-inducing protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052813">
        <property type="entry name" value="CMIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25480:SF0">
        <property type="entry name" value="C-MAF-INDUCING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25480">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="569" mass="63398" checksum="3EA22F947A818685" modified="2018-12-05" version="1">MALTSPLQDESIHQAPLHIISTLLAENSNLSAQDHENIIVAIAPLLENNHPPPDLCEFFCKHCRERPRSMVVIEVFTPVVQRILKHNMDFGKCPRLRLFTQEYILALNELNGGMEVVKKFIHSMHGPTGQCPHPRVLPNLVAVCLAAIYSCYEEFLNSRDNSPSLKEIRNGCQQQNDRKPPMPLRLLRPEPPTPPPSTILPLSSILVELERNNNTTNAKRKGGPAGGDSEPNLIDCLLVSPAVNTLSIQLPAQADRVLGCFALILKMLSDYDDWRPALASLLQPIPFPKEELKYVIQRFAEDPRQEVHSCLLSVRSGKDGWFQLYSPGGVACDDDGELFASMVHILMGSCYKTKKFLLSLAENKLGPCMLLALRGNQTMVEILCLMLEYNIIENKDTQLQIISTLQSTQVGFRMYEQLCDRQRELKELQRKGGPTRLTLPSKSTDADLARLLSSGSFGNLENLSLAFTNVTSACAEQLIKLPSLKQLNLWSTQFGDAGLRLLSEHLACLQVLNLCETPVTDAGLLALSSMKSLCSLNMNSTKLTADTYEDLKAKLPNLKDVDVRYTEAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MB91</accession>
    <name>A0A3B5MB91_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MB91"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021112.1">
        <property type="protein sequence ID" value="ENSXCOP00000020857.1"/>
        <property type="gene ID" value="ENSXCOG00000015619.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01070000254036"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF118">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017391159" description="Immunoglobulin V-set domain-containing protein" evidence="4">
        <location>
            <begin position="23"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin V-set" evidence="5">
        <location>
            <begin position="31"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="226" mass="25032" checksum="4D55AD4CB5CDF90A" modified="2018-12-05" version="1">LRMKVRLTLICLFFLSEYIILSLQDGETLRTYTENEGGSITVRCEFGYSGVKRFLCKKTCEGKNILIVTTEETDQRGRFSIRYEKRNIFSSDFLYVSITDLKPSDSGRYRCCSDETLDGTLYDDFYLVVTKISALKTKLQILDKILCFCRVGPLIRSTFSSSASTTTATTTSRRTTAAATAAATATAATQSQSFSLSPSESVKLSEKPVAASGSPSVHVDEMIWVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBA4</accession>
    <name>A0A3B5MBA4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBA4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021528"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021792.1">
        <property type="protein sequence ID" value="ENSXCOP00000021528.1"/>
        <property type="gene ID" value="ENSXCOG00000016122.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000238283"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02178">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00929">
        <property type="entry name" value="ATHOOK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="100" mass="11242" checksum="C2FA41B668495C8A" modified="2018-12-05" version="1">MSTSRPRKPVVKYSGGVPKTVSKKPHRGRGRPRKNLDDSQPVKRGRGRPKGSTKNNPSPFKARRKQGRPRKYPLPSAEELKKPKETEEDTAGRRGGRCAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MC26</accession>
    <name>A0A3B5MC26_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Adenine phosphoribosyltransferase</fullName>
            <ecNumber evidence="7">2.4.2.7</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:16609"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.7" evidence="7"/>
    <dbReference type="RefSeq" id="XP_027862445.1">
        <property type="nucleotide sequence ID" value="XM_028006644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MC26"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018826"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019062.1">
        <property type="protein sequence ID" value="ENSXCOP00000018826.1"/>
        <property type="gene ID" value="ENSXCOG00000014172.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114137812"/>
    <dbReference type="KEGG" id="xco:114137812"/>
    <dbReference type="CTD" id="353"/>
    <dbReference type="GeneTree" id="ENSGT00390000017259"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00646"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002055">
        <property type="term" value="F:adenine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003999">
        <property type="term" value="F:adenine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006168">
        <property type="term" value="P:adenine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000123">
        <property type="entry name" value="Adenine phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00004">
        <property type="entry name" value="Aden_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005764">
        <property type="entry name" value="Ade_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050054">
        <property type="entry name" value="UPRTase/APRTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01090">
        <property type="entry name" value="apt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002634">
        <property type="entry name" value="PRK02304.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002636">
        <property type="entry name" value="PRK02304.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315:SF3">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="15">
        <location>
            <begin position="35"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00017366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="181" mass="19805" checksum="6919308DA40C920E" modified="2018-12-05" version="1">MAAGPEGKLQLVQRHVRAFPDFPKKGILFRDICPILKSPAALTAVIDLFEERVRSRQLQVDLIVGLDARGFLFGPLLAQRLGVGFVLVRKKGKLPGATVSVAYDLEYGTAEAEIQEDAVAPGQKVLIIDDLLATGGTLLAACELMKKQRVDIQGCMVVIELRDLKGADRLKPHNVFSLLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDK4</accession>
    <name>A0A3B5MDK4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NIPSNAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NipSnap family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDK4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019341"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019579.1">
        <property type="protein sequence ID" value="ENSXCOP00000019341.1"/>
        <property type="gene ID" value="ENSXCOG00000014458.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183018"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000017">
        <property type="entry name" value="Protein NipSnap homolog 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012577">
        <property type="entry name" value="NIPSNAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051557">
        <property type="entry name" value="NipSnap_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21017">
        <property type="entry name" value="NIPSNAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21017:SF19">
        <property type="entry name" value="PROTEIN NIPSNAP HOMOLOG 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07978">
        <property type="entry name" value="NIPSNAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIPSNAP" evidence="3">
        <location>
            <begin position="89"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="NIPSNAP" evidence="3">
        <location>
            <begin position="145"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="246" mass="27555" checksum="BD2C66014A29B54A" modified="2018-12-05" version="1">QNRTRTQNQRRRGAAAPHQAPPPDLKQERTGRVRLTQQNRQRNLIPSNQIRPMWSADQSSNCPKVQTAGSGKSELLDVTTNYSEKKNEKDYSQRAAVRAALAQDPSWISEYICKALPLLTAQDNEVSYLVPWSRLKSPPQQGGVFELATFRMRPGGPAVWGGAFQAAMSAHDAPGYGKLLGAFHSEFGLLNRVHALWWFESADHRAECRHKAHTDARVVAAVRNSVAHLDSQRNQLMFPCTFSPMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDY0</accession>
    <name>A0A3B5MDY0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Titin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDY0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019701.1">
        <property type="protein sequence ID" value="ENSXCOP00000019461.1"/>
        <property type="gene ID" value="ENSXCOG00000014616.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000267173"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Titin a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000050">
        <property type="entry name" value="Titin isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14340">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED GLYCOPROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14340:SF13">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="28"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="128"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="226"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="11">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="45191" checksum="A7F3F714807D30E1" modified="2018-12-05" version="1">MTLFLYHFDFILYYTCNLFFILIYKESLKIMVPPEDIDTQEKKTISFSCKTNRPNATVKWMKAGQEITFSKRIVYRVDKEKHTLTIKDCTLADEGEYTVVAGDDKATNKSDPMASLELPPSVCCTVPPRVELGVNMKSLIVVKAGDNVFLDAEVHGKPLPKVSWKKDGAPLVIAEGMKATQKKHVHLLELYSVTRKETGDYTVLAENLSGSKYATIKVKVDDIEEPRLDIDASYSSNVVVMAGEPFKLEASVTGRPIPSLVWTKEGKELEDTAKIEIKTSDFHTTLISKDSLRRDGGAFTLTASNPGGFAKFTFNVKGRPAPEVKWSREHGESLDKANIDITPSFTTLQIDNVDRFDGGKYLVTVENASGSKTAFVNVRVLDTPGPPQNLIVKEVTRDSVSLVWDAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MF21</accession>
    <name>A0A3B5MF21_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane protein 255A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Protein FAM70A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM255 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MF21"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022444.1">
        <property type="protein sequence ID" value="ENSXCOP00000022175.1"/>
        <property type="gene ID" value="ENSXCOG00000016566.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160471"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028014">
        <property type="entry name" value="TMEM255"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33721:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 255A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33721">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 255B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14967">
        <property type="entry name" value="FAM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="280"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="280"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="305"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="319" mass="34817" checksum="25ED2BD3C3528677" modified="2018-12-05" version="1">FDQHVTQQMHGSFKRRKRKSITVTVMLLIVSVLILVFGLAATTRTQNITVGGYYPGVILGFGSFLGIIGAHLIENKRQMLVASIVFISFGAVAAFCCAIVDGVFAARHIDLRPLHAGRCGYLSSDSASERDVRHLYYISLSFHALNVPCHTPRSSCKLHVKSNTCYCCDLYNCGSRAEVMGGYHEYTDVKSCQDVVHLYYLLWSATILNIVALFLGIITAAVLGGFKDMVETASEPEAIAAPAPPAPPPTTSLNLYHNTAPCLPPYTAYDLQGPFLFPDSSGLSDDSQSGASHLWPTMVPPRYSPPHEHPDEKPPPYSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGI3</accession>
    <name>A0A3B5MGI3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGI3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022852"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023127.1">
        <property type="protein sequence ID" value="ENSXCOP00000022852.1"/>
        <property type="gene ID" value="ENSXCOG00000017077.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177567"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="112" mass="11756" checksum="C35F91C018426773" modified="2018-12-05" version="1">GATSEGTTRGGETEQCDGPSQAQAGRRQKKGRAELISIPECSPDNEIEHGLMSHNQALKDLMDLTGSVTHTKLPSESTIGDCNKNLIRGVVSPMTDSKLIGVAPPSSNLLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHE6</accession>
    <name>A0A3B5MHE6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHE6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023182"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023460.1">
        <property type="protein sequence ID" value="ENSXCOP00000023182.1"/>
        <property type="gene ID" value="ENSXCOG00000017321.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048311"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013:SF7">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46013">
        <property type="entry name" value="VASCULAR CELL ADHESION MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017365720" description="Ig-like domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="16"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="122"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="272" mass="30448" checksum="91FFF7B5B5FB1EB9" modified="2018-12-05" version="1">IKMKLMVSIALLFLLPSYAIVHIESTPKPSTDGVIQTETEKIVSLVCILTGTVDNKELEWRRNGAVIKLDDGNKENRSSVCINPVIYKDEGAKFTCQLKMSIVFLKFCSDLAEISGSENVTLEEEESLDLQCDMRANPLVTSVTWKLNGALVDLSTGGFIVSNNGVNTKLHVKKVDRSLHEGEYQCTIASPAYEERSRTFQVTVTDKTLKFPLMPMIAGLVVVGLTALLAIVSRWRIIARVTHAKQTQTLLIRILQHKLLKSFILLQQWFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIF4</accession>
    <name>A0A3B5MIF4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SIS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIF4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023405"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023686.1">
        <property type="protein sequence ID" value="ENSXCOP00000023405.1"/>
        <property type="gene ID" value="ENSXCOG00000017473.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005345"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042593">
        <property type="term" value="P:glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009750">
        <property type="term" value="P:response to fructose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054017">
        <property type="entry name" value="GKRP_SIS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005486">
        <property type="entry name" value="Glucokinase_regulatory_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040190">
        <property type="entry name" value="MURQ/GCKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10088">
        <property type="entry name" value="GLUCOKINASE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10088:SF4">
        <property type="entry name" value="GLUCOKINASE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20741">
        <property type="entry name" value="GKRP-like_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22198">
        <property type="entry name" value="GKRP_SIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22645">
        <property type="entry name" value="GKRP_SIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01272">
        <property type="entry name" value="GCKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SIS" evidence="2">
        <location>
            <begin position="276"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="512" mass="56857" checksum="B5561903DDA6D843" modified="2018-12-05" version="1">MSNNAKASPWSQRPPEEACLMKKMAHVVEREFLFQSTDYEPTLPVTEKSNPLTRDIDRASASSIVKMLQACDAQMFQEKLLVFFKNSGFNRELQRLNYAPICSYVIAGGDRALFSSQEAPEDDPTLGALRLKKVSTIPCLRGKKRVLFIGVSCGLSAPFVAGQLDFCLRHPDVYTPVLVGFNPCVSLCRNEPLPGCTLTFRSVVTRMEELAKTQKAFLINPALGPEAISGSSRMKGGSATKILLEVVFSASFSRTPVTFKYAQWGYGRAVQKILFVCFSLQCGRQVCYLGWGSLGLLGLIDASECKPTFGHSELLFPQGPEFSISHDDFLDRVLPRLTDDDAVLLLYSDSDDVDEVAKLAHRVREKTSNIHGAYHQTDGGTDDINKLCSSTINFTWSQETPKRLQHMRELSTKLLLNAVSTGAHILKGKVFKNYMIDLQVTNSKLYRRAARLLQVRIQSDVEMNQHLCVSVVQVVPLALVCLLTGCSIKEAETRLEQQPIVREAVEACLKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJA0</accession>
    <name>A0A3B5MJA0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJA0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024413"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024707.1">
        <property type="protein sequence ID" value="ENSXCOP00000024413.1"/>
        <property type="gene ID" value="ENSXCOG00000018200.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156444"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06799">
        <property type="entry name" value="PDZ_MPP3-MPP4-MPP7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.650">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050716">
        <property type="entry name" value="MAGUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23122">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE MAGUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00569">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="L27" evidence="7">
        <location>
            <begin position="77"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="148"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="236"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="5">
        <location>
            <begin position="377"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="584" mass="65577" checksum="504D6D2A66E0C3AD" modified="2018-12-05" version="1">PGVTDLGATPSSKQACFCYVMLAGISEVLSSVVEDVSEAVNRNISGSQLLHELLSAPWLRALLKIYECLMRFQRLSPSPLLPCASGLLFEVRKAHRPSAEARELYNLLSSPHVQALLSSHDSVAQSDYLPVLSPLPDELPEDEEAMRIVCLVKNNQPLGATIRREEVTGDIYIARVIHGGLADRSGLLHPGDLIVEVNGNSVVGLEPEKVIQKLNSSIGTILFKVIPRSTQVTSSQKPVYMRAMVDYCPLQDSSIPCPDAGMAFSRGDLLEVVDQSDALWWQARKLPCGASFAGLIPSASMLRSKHKEQWWCHPSQVHTCIRPCENISGFRRSFRLWRRTSYKRRRKSCTSCSPSSGALSSPYEEVALYQRLPQENHRLIILIGASGVGVNELRKRLIKLNPSTFQGPVPHTTRPIKEGEQAGREYHFVTKELFEYMVCNHRFVEYGESKSHLYGTSIDAIDEVIKRGQTCIIDVEPPGIHLLRLRNLKPYVIFIRAPSPDTLRQTRRNARLITSYAVNRAFTEEDFLELEDTSQLIDAKYKQYFDSVVVNDDLQDACMQLCSIIQQAQNEPQWIPVSWLHGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJP2</accession>
    <name>A0A3B5MJP2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein flightless-1 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027898232.1">
        <property type="nucleotide sequence ID" value="XM_028042431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJP2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023992"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024277.1">
        <property type="protein sequence ID" value="ENSXCOP00000023992.1"/>
        <property type="gene ID" value="ENSXCOG00000017914.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114160062"/>
    <dbReference type="KEGG" id="xco:114160062"/>
    <dbReference type="CTD" id="2314"/>
    <dbReference type="GeneTree" id="ENSGT00940000156643"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030239">
        <property type="term" value="P:myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11280">
        <property type="entry name" value="gelsolin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd11290">
        <property type="entry name" value="gelsolin_S1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11292">
        <property type="entry name" value="gelsolin_S3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11288">
        <property type="entry name" value="gelsolin_S5_like"/>
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    </dbReference>
    <dbReference type="CDD" id="cd11291">
        <property type="entry name" value="gelsolin_S6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000033">
        <property type="entry name" value="FLII, actin remodeling protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000050">
        <property type="entry name" value="FLII, actin remodeling protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000054">
        <property type="entry name" value="FLII, actin remodeling protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000019">
        <property type="entry name" value="protein flightless-1 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000020">
        <property type="entry name" value="protein flightless-1 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000030">
        <property type="entry name" value="protein flightless-1 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000031">
        <property type="entry name" value="protein flightless-1 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000034">
        <property type="entry name" value="protein flightless-1 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000021">
        <property type="entry name" value="protein flightless-1 homolog isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="Severin"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007122">
        <property type="entry name" value="Villin/Gelsolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977:SF51">
        <property type="entry name" value="PROTEIN FLIGHTLESS-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11977">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00597">
        <property type="entry name" value="GELSOLIN"/>
    </dbReference>
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        <property type="entry name" value="GEL"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
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        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
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    </dbReference>
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        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="17">
        <location>
            <begin position="241"/>
            <end position="323"/>
        </location>
    </feature>
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        <location>
            <begin position="507"/>
            <end position="588"/>
        </location>
    </feature>
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        <location>
            <begin position="627"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="16">
        <location>
            <begin position="760"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="16">
        <location>
            <begin position="1177"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="950"/>
            <end position="975"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1268" mass="144507" checksum="CD40B30ADD6405A1" modified="2018-12-05" version="1">MAATGVLPFIRGVDLSGNDFKGGYFPEHVKCMSSLRWLKLNRTGLCYLPEELASLQKLEHLSVSHNSLTTLHGELSSLPNLRAVVARANSLKNSGVPDDIFQLEDLSVLDLSYNQLTEIPRDLENSRNMLVLNLSHNSIDSIPNQLFINLTDLLYLDLSDNKLDSLPPQMRRLVHLQTLTLNNNPLMHAQLRQLPAMVALQTLHLRNTQRTQSNMPTSLEGLTNLADVDLSCNDLTRVPECLYSLSSLKRLNLSSNQISELSLCIDQWTQLETLNLSRNQLTSLPSAICKLSKLKKLYVNSNKLDFDGLPPGVSKLCSLTEFMAANNNLELIPEGLCRCDKLKKLVLNKNRLVTLPETIHFLTELEVLDVRENPNLVMPPKPADRAAEWYNIDFSLQNQLRLAGASPAVVAAAGGGASPRDHLARKMRLRRKKDSSQDDQAKQVLKGMSDVAQEKKNMEENGDLKYGDLKVRRWDKSLEKPQLDYSEFFMEDVGQIPGVSVYQIENFVPLQVDEAFHGKFYEADCYIVLKTFLDDNGALNWHIYYWIGQEATLDKKAGSAIHAVNLRNFLGAECRTIREEMGDESEEFSAVFNNEISYIEGGTASGFYTVEDTHYTIRLYRVYGKKNIKLESVPAKGTSLDPRFVFLLDTGLEIFIWRGANATLGGTTKARLFAEKINKNERKGKAEIVTLMQNQEPPGFWEALGGQPEEIRKHVPDDFSSIRPKLYKVGLGLGYLELPQINYKLSVEHKDHKIKLDTLPELRLVQSLLDTKGVYILDCWSDVFIWIGRKSPRLVRAAALKLGQEVCSMLHRPKHACVTRNLEGTECQVFKSKFKNWDDVLKVDYTRAAETVQQKDNLQGKVKKDAEQKDQMKTDLTALFLPRQPAMPLTEAEQLMEEWNEDLDGMEGFVLEGKKFTRLPEEEFGHFYTQDCYVFLCRYWVPVEYEDEDEKKDGEAGKGRGGGGKGDEDEEDKQPEEDFQCVVYFWQGRQASNMGWLTFTFSLQKKFESLFPGKLKVVRMTQQQENLKFLSHFKRKFIIHKGKRKQNTDSVQPSLYHIRTNGSALCTRTIQIGTDSSNLNSEFCFILKVPFESTDNQGIVYTWVGRAADPDEAKLAEDIMNSMFDDTYSKQVINEGEEPENFFWVGIGSQKEYDEDAEYMKYARLFRCSNEKGYFSVSEKCSDFCQDDLADDDIMLLDNGKEVYMWVGTQTSQVEIKLSLKACQVYIQHMRAKDSEHPRKLRLVRKGNEPHCFTRCFHAWGPFKAPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKU2</accession>
    <name>A0A3B5MKU2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the copine family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKU2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020139"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020386.1">
        <property type="protein sequence ID" value="ENSXCOP00000020139.1"/>
        <property type="gene ID" value="ENSXCOG00000015090.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159679"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01459">
        <property type="entry name" value="vWA_copine_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF133">
        <property type="entry name" value="COPINE-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="5">
        <location>
            <begin position="233"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="469"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="471"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="488" mass="54519" checksum="3799FED84119228D" modified="2018-12-05" version="1">MNNTFLNMASIGDFDPLNSSIPATKVEITVSCRNLLDMDTFSKSDPSKRHDFGRTEVIDNTLNPDFVRKFMLDYFFEERQNLRFDLYDVDSKSANLQKHVSPSLDLPFSHCIQGKKCGTIIVKAEELNNCRVSNWFTICHKTEVVKNTLNPVWQAFKIPVRALCNGDYDSLLFFICPCSHDYIGEFSTSYRELSIGQSQFNIYEVSLNVTLLSFLVDVEVTFLDYIKGGTQINFTVAIDFTASNGNPAQPTSLHYMSPYQLNAYAMALKAVGEIIQDYDSDKMFPALGFGAKLPPDGRVSHEFALNGNPQNPYCAGIDGVMEAYYQSLKSVQLYGPTNFSPVINHVARYAASVKDGSQYFVLLIITDGVISDMAQTKESIVNASCLPMSIIIVGVGPAEFDAMVELDGDEVRISSRGRFAERDIVQFVPFRDYIDRSGNHILSMARLAKDVLAEIPDQFLSYMRTRGIKPSPAPPPYTPPGQPVQTQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MMN6</accession>
    <name>A0A3B5MMN6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nematocyst</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Target cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MMN6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022406"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022680.1">
        <property type="protein sequence ID" value="ENSXCOP00000022406.1"/>
        <property type="gene ID" value="ENSXCOG00000016740.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042151">
        <property type="term" value="C:nematocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044218">
        <property type="term" value="C:other organism cell membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051715">
        <property type="term" value="P:cytolysis in another organism"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006812">
        <property type="term" value="P:monoatomic cation transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046931">
        <property type="term" value="P:pore complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.270.20">
        <property type="entry name" value="Cytolysin/lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Actinoporin_PFT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009104">
        <property type="entry name" value="Anemon_actinoporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015926">
        <property type="entry name" value="Cytolysin/lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40388">
        <property type="entry name" value="BRYOPORIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR40388:SF3">
        <property type="entry name" value="DELTA-ACTITOXIN-AEQ1C-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06369">
        <property type="entry name" value="Anemone_cytotox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63724">
        <property type="entry name" value="Cytolysin/lectin"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-0166">Nematocyst</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1052">Target cell membrane</keyword>
    <keyword evidence="4" id="KW-1053">Target membrane</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="152" mass="17073" checksum="1EDD0959073AA1E0" modified="2018-12-05" version="1">MAHLSCLNSLVYMTSGFSYHPPQPTVRTQKTEVCSFTKDDNTATGAVGLLTYDLFHMQSRVCSERMALMFSVPFDHTIYKNRLAVGVVEHSRACDKHLYDQMYDGKDLSNFTRSEANGCGLEYKAAHVDLRATMSTTGKAIVKVELYDKMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNI8</accession>
    <name>A0A3B5MNI8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNI8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025353"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025660.1">
        <property type="protein sequence ID" value="ENSXCOP00000025353.1"/>
        <property type="gene ID" value="ENSXCOG00000018937.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017319898" evidence="2">
        <location>
            <begin position="22"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="90" mass="9621" checksum="BD2B09E5799B10CD" modified="2018-12-05" version="1">IFFFFLAFDIFWNIFVQPMFSGINGMPGMIGFRGLEGAPGLPGEEGRPGPAGKYGFPGDPGRSGPPGPAGTTGEPGWRSRHTGIFFKDQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPM9</accession>
    <name>A0A3B5MPM9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MPM9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025743"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026053.1">
        <property type="protein sequence ID" value="ENSXCOP00000025743.1"/>
        <property type="gene ID" value="ENSXCOG00000019240.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155624"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000264">
        <property type="entry name" value="ATP-binding cassette, sub-family A (ABC1), member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF36">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="21"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="380" mass="42730" checksum="D5B352FBF630A468" modified="2018-12-05" version="1">DIDPQHHRSSTQDPLGLVLGVQIQDLVKVYDGSSRPAVNCLNICFYEGQITSFLGHNGAGKTTTLSILTGLYPPTSGTAYINGRDIKTDIDIIRTSMGMCPQYNILFNHLTVEEHILFYSLLKGRTPEEAEAEVENMLLDLGLPHKRDEEAQNLSGGMQRKLSVAMAFVGGSKVVILDEPTSGVDPYSRRSIWDLLLKYRTGRTVILSTHHMDEADLLSDRIAIISKGQLYCCGSPLFLKNCFGVGFYLTLVRRMKDIRKREVSSACRQRTLFPPLLSVFIAFVVESARVFLCFCQNECDCASDCSCSCSICTKYKDQSQNQHQQLDRVLDGKCSCSLSTFHLHAHKQHFLQKLHLQSSLFLFVVKDIIVLFFAFPYLCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQ13</accession>
    <name>A0A3B5MQ13_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Capping protein regulator and myosin 1 linker 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CARMIL family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000021574"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021838.1">
        <property type="protein sequence ID" value="ENSXCOP00000021574.1"/>
        <property type="gene ID" value="ENSXCOG00000016146.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155112"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034315">
        <property type="term" value="P:regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000009">
        <property type="entry name" value="F-actin-uncapping protein LRRC16A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031943">
        <property type="entry name" value="CARMIL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041245">
        <property type="entry name" value="CARMIL_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051279">
        <property type="entry name" value="PP1-Reg/Actin-Interact_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112:SF39">
        <property type="entry name" value="F-ACTIN-UNCAPPING PROTEIN LRRC16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24112">
        <property type="entry name" value="LEUCINE-RICH REPEAT, ISOFORM F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17888">
        <property type="entry name" value="Carm_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16000">
        <property type="entry name" value="CARMIL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CARMIL pleckstrin homology" evidence="11">
        <location>
            <begin position="8"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="CARMIL C-terminal" evidence="10">
        <location>
            <begin position="730"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="896"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="989"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1090"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1246"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1024"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1102"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1129"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1142"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1262"/>
            <end position="1287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1287" mass="141777" checksum="0E4297E184D3F94B" modified="2018-12-05" version="1">SRFFLFLQVEHSFNYLEIQGIASSKPTQLLVEYERGSFSVKLLSTEEVNDVVAHVGNCLLRICPGLPPSKVMKKLCMEPPDRLSYLQSLWENKPAEPGPCGGFSQMYRCVCDWLGLPYREEVQWDVDTIYLTQDSRELNLQDFSHLENRDLVAIIAVLEFNQWFTKLSTKDYKLSADVCEQILRVVSRSSRLEELVLDNAGLKTEFAQKLAAALAHNTSSGLASINLANNPLEDRGVSALGVQFAKLRLGLKHLNFSKTSISPKGVNSLCQSLSANPSIASSLVHLDLSGNMLRGDDMQHFYNFLGQPNSLATLDLSNTDCTLDQVCSALLRGSAQHLSVLNVSKSIFPHRKGKEVPPSFKHFFSSATSLSSINVSGTKLPPEALKALLLGLASNPSLKDVSLDLSCCELRSGGSQILEGCIAEIPNISSLDLSDNGLDSELSTLLVWLAKNRSIKHLSLGKNFNNIKSKNLAPVLDGLVHMIQEEESPLTSLSLADSRLKSDLTIVLNALGSNSTLTRLDISGNAMGDMGAKMLAKALQINSKLRTVIWDKNNTSPQGLQDVATALEKNFTIRFMPIPIIDASQALKASPEKTEDALLKIETYLYRNHETRKYLQEQAYRLQQGIVTTTTQQMIDRICVKVQDHLNSLKNSEADAILEDVRSAENLIKDARNSKTLLRNLYHLGSASREELNSSSAGPIQEKLESMAGEVTTVMDQQLQSLLESMMDTAESLCPHVMKRGNLRPELMKASSTKMVVPKCFVTQTLLEQSGVDIINKISEVKLSLASFLSDRIVDEILEALSTSQLTLADHLVQRGRRLVQQESMDLDVPEEKIPKRASAEILEAERLEDLETCMMTPKSKRKSIHSRMLRPVSRAFEMEFDLDKALEDVPIYMDDASTSSQTPPTPSVGGPKAEQRPTGAIAELPSEEEKKLQHFTKLRPRRNKKMHSSKVAVSKTLLLFYDLNTNGGEQNGLMGRVDEGVDEFFSKKVTKMDSKRSSKSSESQDGEKKKSKGGFLNLIKRSSKSDKSDKSDKSDKSEKDRSEELRTPDSIDEPWEGSDGRGSPQGGKRYTGVQMMGSGLLAEMKAKHERRAYKVSNVRWTKSQSSTTESKTSSCPTNKANAAAAEKSSPHSEAKSEPSARPRATSSSTSPAGPVNPKPPVPQGAKPALAARPTIPQKPRANSSRSIGMIHSLHKLSAALPGFLNHFKNRQILQRYVSCLFFHIDESSASPIGSGHVSPKILPSTLKRTMSEKDKEPILQTGVSTNKSSQEGESQNQLKLSVSNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQM7</accession>
    <name>A0A3B5MQM7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMA7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQM7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023481"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023762.1">
        <property type="protein sequence ID" value="ENSXCOP00000023481.1"/>
        <property type="gene ID" value="ENSXCOG00000017533.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003710"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015157">
        <property type="entry name" value="TMA7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28632">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09072">
        <property type="entry name" value="TMA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="64" mass="6985" checksum="791B192F08D7BDF7" modified="2018-12-05" version="1">FIHFSGGKKKPLKAPKKQNKEMDDEDVAFKQKQKEEQKAMEALKAKASGKGPLASGGIKKSGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MT27</accession>
    <name>A0A3B5MT27_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the reduced folate carrier (RFC) transporter (TC 2.A.48) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MT27"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027559"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027893.1">
        <property type="protein sequence ID" value="ENSXCOP00000027559.1"/>
        <property type="gene ID" value="ENSXCOG00000020564.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183022"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008518">
        <property type="term" value="F:folate:monoatomic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005542">
        <property type="term" value="F:folic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098838">
        <property type="term" value="P:folate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000225">
        <property type="entry name" value="Solute carrier family 19 member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002666">
        <property type="entry name" value="Folate_carrier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00806">
        <property type="entry name" value="rfc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10686">
        <property type="entry name" value="FOLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10686:SF12">
        <property type="entry name" value="REDUCED FOLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01770">
        <property type="entry name" value="Folate_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028739">
        <property type="entry name" value="Folate_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="322"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="347"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="406"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="458"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR028739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="492" mass="54284" checksum="5334089DD63B65B8" modified="2018-12-05" version="1">MGADGAAAGKDVDLKTAPPLEDGEGRGDPEAAAVPCPSEDVGKLTYSVMFLCFYGFMASIRPGESFITPNLLSSEKNFTREQVTNEITPVLTYSYMAVLVPAFLLTDLLRYKPVLIIQGVSHVVIWVLMLLGATLLHMQLMEFFYGITMACRVAYSSYIFSLVSPVLYQRVAGYSRSSVLLGVFVSSVLGQLCLSLGDISYYTLDAISLGFVSFGLALACCLPWPKRSLFFNRTKRQEPAEKAAPSETDEMNPRRAGLSSAARWKESIFVQMLLEVGNMVKRPNLRLWSLWWIFNSTGYYLVLFYVHILWNKVHSTANYNGAVEAAATLLSAITSFMAGFAKIRWNIWSELVIGGITALQAGMLLVMGTTGNIWVCYSCYILFRGFYQFLVPIATFQIASSLTRELCALVFGINTFLGTVLKSIINLIFSDKRGLGLDVTSQVSAAVVLVRHFRTQNRGEEEASPDGLHTELTPVSMTSEEEPLSNSQSVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MTH4</accession>
    <name>A0A3B5MTH4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MTH4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022789"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023065.1">
        <property type="protein sequence ID" value="ENSXCOP00000022789.1"/>
        <property type="gene ID" value="ENSXCOG00000017031.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183146"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF159">
        <property type="entry name" value="CD180 MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017443348" description="Ig-like domain-containing protein" evidence="6">
        <location>
            <begin position="28"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="276"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="388"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="388"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="400"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="425"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="442"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="460" mass="50049" checksum="C4A2C201A034D44F" modified="2018-12-05" version="1">MLAAVLQGVQWPVVVLMVCVQLSAVRGAACPPRCLCTADILSCPALDLDRVPAALPASTATMDLSHNRIQQLEPGGFQGLLQLEVLRLAHNQLSAIQPGAFRNSSGRLKHLDLSSNQLLVLKQSDFQELAALEELLLFNNRIKHVESTALSGLTGLRKAYLSHNRLTDFPFFSIQTGGHQQLTLLDLSSNRLPKLPLMDISGLPLALQGGLYLHNNSLVCDCAMFSLFRLWEQKGFASVRFFRQEHVCLVAGVQRSGIRFFLHRRYFERCNVTAAPQQEASVSVRAGAPLLLHCVTSLSGRGAAFFWLTPQLEYVAPPGNNGSIRMFANGSLAMAAPQEQDSGIYVCVAQARRLNDSREVNVTVLPSICLTPALPSLPAPLPSLPAPPSLPAPLSPPTPSISNPSSSSSSSSRQEALRRSSTCMRKTEEEPERSLHHTLHHSLHESSSCHRVRADIGDCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MTX5</accession>
    <name>A0A3B5MTX5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ras-related protein Rab-25</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB25 regulates epithelial cell differentiation, proliferation and survival, thereby playing key roles in tumorigenesis. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia. Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions. May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts (GTP-bound form) with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4. Interacts (via the hypervariable C-terminal region) with ITGB1 (via the cytoplasmic region); the interaction is GTP-dependent. Interacts with ITGAV. Associates with the integrin alpha-V/beta-1 heterodimer. Interacts with VPS33B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Pseudopodium membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MTX5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026946"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027277.1">
        <property type="protein sequence ID" value="ENSXCOP00000026946.1"/>
        <property type="gene ID" value="ENSXCOG00000020125.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158230"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031260">
        <property type="term" value="C:pseudopodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000067">
        <property type="entry name" value="ras-related protein RABA1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="217" mass="24241" checksum="3E7DB7210B6F8248" modified="2018-12-05" version="1">MGSDDSYNFVFKVVLIGESGVGKSNLLSRFTKNEFLHDSRTTIGVEFSTRTVQLDNFTIKAQIWDTAGLERYRAITSAYYRGAVGALLVYDISKHLTYESAERWLKELYDHADPHMVVMLVGNKKDLESLRTVPTEEAKDFAGELNCLMFMETSALDSTNVEAAFNEVLTGKKRHTIHKKVASREVTRGSISAVTLSSPLGLTREAQEERKGCCKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MU26</accession>
    <name>A0A3B5MU26_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">GDNF family receptor alpha 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MU26"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022984"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023261.1">
        <property type="protein sequence ID" value="ENSXCOP00000022984.1"/>
        <property type="gene ID" value="ENSXCOG00000017177.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156168"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016017">
        <property type="entry name" value="GDNF/GAS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003438">
        <property type="entry name" value="GDNF_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269:SF4">
        <property type="entry name" value="GDNF FAMILY RECEPTOR ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269">
        <property type="entry name" value="GDNF RECEPTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02351">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00907">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="7">
        <location>
            <begin position="46"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="179" mass="20082" checksum="5D1BD5E9C7C24569" modified="2018-12-05" version="1">MSSILETLNRTILKIVYFLVVIWSYVPLKNVLSALGSPGWREPPDCMRASELCNQNSQCSSRYRIMRQCLSGGDKERSTMLASKECQGALEVLQDSPLYDCRCKRGMKKELQCLQNYWSIHMGLTEVATVMSAMMVDITALCCLASCSGREGENSHGVRLVLGYFLLVALWSVFIVECC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MU52</accession>
    <name>A0A3B5MU52_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MU52"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023009"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023286.1">
        <property type="protein sequence ID" value="ENSXCOP00000023009.1"/>
        <property type="gene ID" value="ENSXCOG00000017195.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157432"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000003">
        <property type="entry name" value="Adhesion G protein-coupled receptor B2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.100.10:FF:000004">
        <property type="entry name" value="Adhesion G protein-coupled receptor B2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052065">
        <property type="entry name" value="Compl_asym_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22906:SF52">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22906">
        <property type="entry name" value="PROPERDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01705">
        <property type="entry name" value="TSP1REPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="260" mass="28789" checksum="2BE91D51808CC80A" modified="2018-12-05" version="1">MDLSFSSFSSQSFHSLPIPSCSVESTSGEEWSSWSVCSATCGEGWQSRTRFCVSSSYSTHCSGPLREQRPCNTSAICPVHGTWDEWSPWSLCSSTCGRGYRARTRTCSPPQFEGKPCDGPGKQTKFCNIAVCPGKHDPSTNESQLMDGVWNEWSSWSSCSATCSNGTMQRTRECNGPSYGGSECRGEWLETVDCFLRDCPVDGKWQPWSLWSGCSKTCGGGTQQRNRVCYGPFFEGQDCPGERQQIRHCNEKRCPASKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUJ2</accession>
    <name>A0A3B5MUJ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BICD family-like cargo adapter 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Bicaudal D-related protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Coiled-coil domain-containing protein 64B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MUJ2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023149"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023427.1">
        <property type="protein sequence ID" value="ENSXCOP00000023149.1"/>
        <property type="gene ID" value="ENSXCOG00000017289.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160797"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055107">
        <property type="term" value="P:Golgi to secretory granule transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051149">
        <property type="entry name" value="Spindly/BICDR_Dynein_Adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123">
        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123:SF11">
        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="368"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="55"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="194"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="368"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00041790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00043196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="411" mass="46997" checksum="24B3B783E0322051" modified="2018-12-05" version="1">MFTPRKNSLPSPSLEDSFFPLSSSSSVSLFALQSASSPADGVDGGGAIETDLILAAELGRALLEKNEELAETLQQREKEVEALQQEKHVLQRKLEMNELESGQKESELNTDIASLRAELQKYHSEGRDRRKDDSEQMTQLTNHNQRLVEQLSEAVTLEHSLRTELRTLREEMEESSFSRNISFTQLENMQAEQNRVLQERLSHMETQLKASEEDSQRLRVERDGLRDRLSALHVKLTEKEAEIEQEQGVVFELRTLNRSLQQKHLNLGEESFLGSTHTQPLSLLSEIQQTQATEALLAHSSALQAKDEQIQALKDESLESELAKVRQEKESLTQQLLNTIKHKVVLSQELDAWQEDMRFVINQQVLQREEEKKKKEEKQEKESTVGLHRSKSLRVRGEGGKGFFSSLFKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVY5</accession>
    <name>A0A3B5MVY5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Essential for the control of the cell cycle at the G2/M (mitosis) transition.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with the CDK1 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclin family. Cyclin D subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027870710.1">
        <property type="nucleotide sequence ID" value="XM_028014909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVY5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023744"/>
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        <property type="protein sequence ID" value="ENSXCOP00000023744.1"/>
        <property type="gene ID" value="ENSXCOG00000017732.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114143123"/>
    <dbReference type="KEGG" id="xco:114143123"/>
    <dbReference type="CTD" id="595"/>
    <dbReference type="GeneTree" id="ENSGT00940000157816"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20573">
        <property type="entry name" value="CYCLIN_CCND1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000120">
        <property type="entry name" value="G1/S-specific cyclin-D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="9 13" id="KW-0195">Cyclin</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Cyclin-like" evidence="15">
        <location>
            <begin position="62"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="16">
        <location>
            <begin position="155"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="262"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="292" mass="32954" checksum="9DE8E16DC12C19E5" modified="2018-12-05" version="1">MEEQLLCCEVDSIRRAHQDENLLNDRVLQTMLKAEENYLPSPNYFKCVQKEIIPKMRKILATWMLEVCEEQKCEEEVFPLAMNYLDRFLSVEATRKTRLQLLGATCMFLASKMKETVPLTAEKLCIYTDNSVLPGELLQMELLVLSKLKWDLASVTPHDFIEHFLSKLKIHPSTKQILRKHAQTFVALCATDVNFIASPPSMVAAGSVVAAVQGLYLKSQDASLSSPNLTNFLSQVIRSDPDCLRSCQEQIESLLESSLRQAQQHGGAAETKRMDEDADLSCTPTDVRDVNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWC9</accession>
    <name>A0A3B5MWC9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL23</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">60S ribosomal protein L23a</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Binds a specific region on the 26S rRNA. May promote p53/TP53 degradation possibly through the stimulation of MDM2-mediated TP53 polyubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the large ribosomal subunit. Interacts with LYAR and GNL2. Interacts with MDM2; this interaction may promote MDM2-mediated p53/TP53 polyubiquitination. Directly interacts (via BIB domain) with IPO5, IPO7, KPNB1 and TNPO1; these interactions are involved in RPL23A nuclear import for the assembly of ribosomal subunits. Interacts with IPO8.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWC9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023939"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024224.1">
        <property type="protein sequence ID" value="ENSXCOP00000023939.1"/>
        <property type="gene ID" value="ENSXCOG00000017877.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182901"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044391">
        <property type="term" value="C:ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000082">
        <property type="entry name" value="60S ribosomal protein L23a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_uL23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019985">
        <property type="entry name" value="Ribosomal_uL23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001014">
        <property type="entry name" value="Ribosomal_uL23_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005633">
        <property type="entry name" value="Ribosomal_uL23_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011118">
        <property type="entry name" value="PRK14548.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03636">
        <property type="entry name" value="uL23_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03939">
        <property type="entry name" value="Ribosomal_L23eN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00050">
        <property type="entry name" value="RIBOSOMAL_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0164">Citrullination</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL23 N-terminal" evidence="13">
        <location>
            <begin position="16"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="19"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="176" mass="20274" checksum="9013AC11D3CBF6E8" modified="2018-12-05" version="1">MAPKAKKEAVPAKTEAKSKALKAKKAVLKGVHSQRKKKIRTSPTFRRPKTLRLRRQPKYPRKSAPRRNKLDHYAIIKFPLTTESAMKKIEDNNTLVFIVDVKANKHQIKHAVKKLYDIDVAKVNTLIRPDGEKKAYVRLAPDYDALDVANKVSFIPADRCCNVLFYRSLYFVTLRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N0E3</accession>
    <name>A0A3B5N0E3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NR LBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5N0E3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026555"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026877.1">
        <property type="protein sequence ID" value="ENSXCOP00000026555.1"/>
        <property type="gene ID" value="ENSXCOG00000019826.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160748"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06948">
        <property type="entry name" value="NR_LBD_COUP-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000020">
        <property type="entry name" value="Nuclear receptor subfamily 2 group F member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01282">
        <property type="entry name" value="COUPTNFACTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="NR LBD" evidence="9">
        <location>
            <begin position="33"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="273" mass="30434" checksum="A2BDBD5E31C954C4" modified="2018-12-05" version="1">PKSCFLYPPSPCLLPPGPPTKLQQFNAGESHLSRASLISQLLRAEPYPNSRYGHQYNQQAGPDNAMGIDNICELAARLLFSTVEWARNIPYFPELPVSDQVALLRLSWSELFILNAAQSALPLHMAPLLAAAGFHSSPMSAERVVSFMDQVRMFQDQVEKLMRLQVDSAEYSCLKAIALFSPDACGLTDSVHVESLQEKAQVALTEYERMQYPSQPQRFGRLLLRLPALRAVPASLISQLFFMRLVGKTPIETLIRDMQLSGSSISWPYVPGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KNK5</accession>
    <name>A0A3B5KNK5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Rho GTPase activating protein 4a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KNK5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000052"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000052.1">
        <property type="protein sequence ID" value="ENSXCOP00000000052.1"/>
        <property type="gene ID" value="ENSXCOG00000000043.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182824"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000026">
        <property type="entry name" value="Rho GTPase activating protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000136">
        <property type="entry name" value="Rho GTPase activating protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000059">
        <property type="entry name" value="Rho GTPase activating protein 4b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051627">
        <property type="entry name" value="SLIT-ROBO_RhoGAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14166">
        <property type="entry name" value="SLIT-ROBO RHO GTPASE ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01887">
        <property type="entry name" value="SPECTRNALPHA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="10">
        <location>
            <begin position="19"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="9">
        <location>
            <begin position="491"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="8">
        <location>
            <begin position="709"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="802"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="810"/>
            <end position="827"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="847" mass="95418" checksum="050939E3F7194016" modified="2018-12-05" version="1">MTSHGKLRREKGSLAEYESQMKDLRVQLTDQIKILDSQVEVKQQQLSDLSEFLRRRGDIETEYARALDKLTESLLSGLRRVWSVLLTQTRLESREHAALGDTCFNTLTLSLTHSAEDAHRLHKKSKEVGIQMQDELLKVTTELQTALKTYNQYHTDCLIAEGKLKEAERLEERHTGKSAELGPGQLAGQRRSSVKKMERLMEKRHGKMQETQLKCTKARNDYLLNLAAANAAMNKYYLQDVSTLIDCCDLGFHLSIERVMKCYLASRWRIQKTEETGLKQLETAVTSLDQSGDRDGLLQQHDSAFCLPFRFNYHPHEGDQVCEVSAESQVRYELETRFQQLQSRLASVTLETEEVSISKTLKATLAALLDSMCDSECNPSPDVSASLSHEPPGGITTTAKLSLAKRRANQQETETYYLTKVKEFLNSSSLASKLQAKHDLLQDAIQKGALHILIQRIRSSLNPTLMQCTTRSIRVRKSRPVSQFCHTLFTTDILSYIESSGQQIPVVVESCIRFINLHGLHHEGIFRVPGSQREVNLIRDAFERGEDPLSDSECDLDSVAGVLKLYFRGLEPPLFPYESYSPLLESAQIRVVFSTFPRPLLIVMRYLFAFLNHVSQYSDENMMQPYNLAVCFGPSLLRGMESDDAVARQPQVNDLVKTMILQHDSIFPGQSELPGPVYEKHMTLDEPITEEGDGETEHLPSEDGQCTRQQHWEAVALFDYVARSPAELSFKQGDPIVLYSKASCDWWRGEVGGVKGLVPHKYISILDVHYIICFPPLVLTQDMISRQMNSVFKELLSRQPPVPSSPFPPSVSATPSSSSSSSIAPAAPTAKKVFGLRRAGPFQSGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQ37</accession>
    <name>A0A3B5KQ37_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Immunoglobulin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQ37"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000582"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000591.1">
        <property type="protein sequence ID" value="ENSXCOP00000000582.1"/>
        <property type="gene ID" value="ENSXCOG00000000507.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000270800"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF118">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Immunoglobulin" evidence="5">
        <location>
            <begin position="23"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="178" mass="19675" checksum="D2A89D4EBD5F03DF" modified="2018-12-05" version="1">SRKPPAQIPSAAALTRLVETLRRSRANREQEGSVTISCPYDSQSVNKLKFLCRGNRPSTCRQQAVITSSNTQNGRFRLSDDRKSRIFTVTISSLTLKDSGSYLCGVQRISGFDVFSAVELKVKVMKRSRVMHGRVSGSENPEGRVRDRQGSATGLSTAEGLGKNLWSKTGRIRNQKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRK2</accession>
    <name>A0A3B5KRK2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNKSR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRK2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001112"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001125.1">
        <property type="protein sequence ID" value="ENSXCOP00000001112.1"/>
        <property type="gene ID" value="ENSXCOG00000000913.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163429"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06748">
        <property type="entry name" value="PDZ_CNK1_2_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09511">
        <property type="entry name" value="SAM_CNK1_2_3-suppressor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000060">
        <property type="entry name" value="Connector enhancer of kinase suppressor of Ras 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049628">
        <property type="entry name" value="CNK1-3_SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010599">
        <property type="entry name" value="CNK2/3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051566">
        <property type="entry name" value="CNKSR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017874">
        <property type="entry name" value="CRIC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12844">
        <property type="entry name" value="CONNECTOR ENCHANCER OF KINASE SUPPRESSOR OF RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12844:SF17">
        <property type="entry name" value="CONNECTOR ENHANCER OF KINASE SUPPRESSOR OF RAS 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06663">
        <property type="entry name" value="CNK2_3_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10534">
        <property type="entry name" value="CRIC_ras_sig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51290">
        <property type="entry name" value="CRIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="4">
        <location>
            <begin position="7"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="CRIC" evidence="6">
        <location>
            <begin position="80"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="5">
        <location>
            <begin position="211"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="366"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="382"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="514" mass="56007" checksum="3256249AFDE5047B" modified="2018-12-05" version="1">MEPVSGWSPQQVEDWMRGLDDSLQPYVATFRQQRVGGQQLLRLGHQDLLALGVARVGHQELVLEAVDLLCSLNYGVESDCLKTLVGKMRAAHRNLSGAVTQRRKNPAYQNKNSHQPSNQFLSAVVELIAAAKSLLGWLDRSPVTSPSDFTATRSTIIQLGLELTSTVQKDCSVFQMEEKILEVSQALNSVCEKTVQATSDPSNTEPSCLEEVHITNVRPGEGLGMYIKSTYDGLHVVTGTTENSPADQTQRIHAGDEVIQVNQQTVVGWQLKYLVEKMRAESGSITLVLKKRPSGTSGMAATPLRNLRWRPPLVQVNAAGRRGKTAILDLYIPPPPAAPYVPLELSASSHPTSPNSSLDADVRQRLADDPPPEVKQLVPIRLRQRSASRGKPRPVSMPVNSVLGVSGSSSKRRTQGRKGKDELHRYLSNERIGAIAEEEPAFGLPSRGSPSVRGVDHIRGSRCLVGADLHHNATMPYQEAASTKKLPAAAQTRAKTGSTSVLGSWLARLRLLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KW91</accession>
    <name>A0A3B5KW91_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Calcium-activated potassium channel BK alpha subunit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KW91"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001914.1">
        <property type="protein sequence ID" value="ENSXCOP00000001886.1"/>
        <property type="gene ID" value="ENSXCOG00000001530.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156880"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005228">
        <property type="term" value="F:intracellular sodium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF14">
        <property type="entry name" value="POTASSIUM CHANNEL SUBFAMILY T MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Calcium-activated potassium channel BK alpha subunit" evidence="11">
        <location>
            <begin position="50"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="378" mass="42123" checksum="6FA7C50822130E43" modified="2018-12-05" version="1">CRYLHLKLNQKYLDHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADQVVCEEEFKYAMLALNCVCPATSTLVTLLVHTSRGREGQQSPEQWQRMYGCCSGNEVYHIRLCDSKFFGEYNGKSFTYASFHAHKKYGVCLIGIKREENKSILLNPGPHHIMAATDTCYYINITKEENSAFIFNQEERKGRPAGGIYDGPSQLPVHSIIASMGTVAIDLQNTSPPNIPGGKLVPPTVNGTGSRRPSIAPVQEIADSAAILPCDLLSDQSEDDTVFTDEKGSSSTEYVKGYPPNSPYIGSSPTLCHLLAEKAPFCCLRLDQGCMHNSFEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRKELNPIVLLLDNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KWR5</accession>
    <name>A0A3B5KWR5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Si:dkeyp-113d7.1</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KWR5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002101.1">
        <property type="protein sequence ID" value="ENSXCOP00000002071.1"/>
        <property type="gene ID" value="ENSXCOG00000001667.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286936"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002281">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000688">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001954">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF23">
        <property type="entry name" value="ZINC FINGER PROTEIN 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="80"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="178"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="181"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="211" mass="23128" checksum="FCBD1DD8D9AA08CC" modified="2018-12-05" version="1">MLSFGGHIRQIIIQIITAFESTDDEENVCRFCGPTPCCWTGWANPTPTLERARCTNCSPPFGQAGNLKRHQLIHTGQKPYVCAHCPKGFTKADDLYRNHVQIHTGEKPFSCSQCGKTFSNSGVLKTHEKIHTGERPFGCTQCGKSFGRLGHLKAHQQIHTGERPYACPRCAKTFSQSGHLKAHEQIHKRERADTASTSSDGGSSAGSSDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KX38</accession>
    <name>A0A3B5KX38_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Alanine--glyoxylate aminotransferase 2, mitochondrial</fullName>
            <ecNumber evidence="28">2.6.1.18</ecNumber>
            <ecNumber evidence="12">2.6.1.40</ecNumber>
            <ecNumber evidence="5">2.6.1.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">(R)-3-amino-2-methylpropionate--pyruvate transaminase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Beta-ALAAT II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Beta-alanine-pyruvate aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">D-3-aminoisobutyrate-pyruvate aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">D-AIBAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">D-beta-aminoisobutyrate-pyruvate aminotransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="40 41">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="40" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="40" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="38">Multifunctional aminotransferase with a broad substrate specificity. Catalyzes the conversion of glyoxylate to glycine using alanine as the amino donor. Catalyzes metabolism of not L- but the D-isomer of D-beta-aminoisobutyric acid to generate 2-methyl-3-oxopropanoate and alanine. Catalyzes the transfer of the amino group from beta-alanine to pyruvate to yield L-alanine and 3-oxopropanoate. Can metabolize NG-monomethyl-L-arginine (NMMA), asymmetric NG,NG-dimethyl-L-arginine (ADMA) and symmetric NG,N'G-dimethyl-L-arginine (SDMA). ADMA is a potent inhibitor of nitric-oxide (NO) synthase, and this activity provides mechanism through which the kidney regulates blood pressure.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(2S)-2-aminobutanoate + glyoxylate = 2-oxobutanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:77339"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:74359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(R)-3-amino-2-methylpropanoate + pyruvate = 2-methyl-3-oxopropanoate + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:18393"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:57700"/>
            <dbReference type="ChEBI" id="CHEBI:57731"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.40"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:18394"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>2-oxobutanoate + L-alanine = (2S)-2-aminobutanoate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:77355"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:74359"/>
            <dbReference type="EC" id="2.6.1.44"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>2-oxohexanoate + N(omega),N(omega)-dimethyl-L-arginine = L-2-aminohexanoate + 5-(3,3-dimethylguanidino)-2-oxopentanoate</text>
            <dbReference type="Rhea" id="RHEA:77363"/>
            <dbReference type="ChEBI" id="CHEBI:35177"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:58455"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>2-oxopentanoate + N(omega),N(omega)-dimethyl-L-arginine = 5-(3,3-dimethylguanidino)-2-oxopentanoate + L-2-aminopentanoate</text>
            <dbReference type="Rhea" id="RHEA:77359"/>
            <dbReference type="ChEBI" id="CHEBI:28644"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:58441"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>3-oxopropanoate + L-alanine = beta-alanine + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:14077"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:33190"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.18"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="26">
            <dbReference type="Rhea" id="RHEA:14079"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>L-ornithine + glyoxylate = 5-amino-2-oxopentanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:77331"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58802"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-ornithine + pyruvate = 5-amino-2-oxopentanoate + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:77327"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58802"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>N(omega),N('omega)-dimethyl-L-arginine + glyoxylate = 5-(3,3'-dimethylguanidino)-2-oxopentanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:77315"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:197308"/>
            <dbReference type="ChEBI" id="CHEBI:197310"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>N(omega),N('omega)-dimethyl-L-arginine + pyruvate = 5-(3,3'-dimethylguanidino)-2-oxopentanoate + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:77307"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:197308"/>
            <dbReference type="ChEBI" id="CHEBI:197310"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>N(omega),N(omega)-dimethyl-L-arginine + 2-oxobutanoate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + (2S)-2-aminobutanoate</text>
            <dbReference type="Rhea" id="RHEA:77351"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:74359"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>N(omega),N(omega)-dimethyl-L-arginine + glyoxylate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:77311"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>N(omega),N(omega)-dimethyl-L-arginine + oxaloacetate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + L-aspartate</text>
            <dbReference type="Rhea" id="RHEA:77343"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>N(omega),N(omega)-dimethyl-L-arginine + pyruvate = 5-(3,3-dimethylguanidino)-2-oxopentanoate + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:77303"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58326"/>
            <dbReference type="ChEBI" id="CHEBI:197301"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>N(omega)-methyl-L-arginine + glyoxylate = 5-(3-methylguanidino)-2-oxopentanoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:77323"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:114953"/>
            <dbReference type="ChEBI" id="CHEBI:197314"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>N(omega)-methyl-L-arginine + pyruvate = 5-(3-methylguanidino)-2-oxopentanoate + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:77319"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:114953"/>
            <dbReference type="ChEBI" id="CHEBI:197314"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>glyoxylate + L-alanine = glycine + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:24248"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.44"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24249"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>oxaloacetate + L-alanine = L-aspartate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:77347"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 39">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.18" evidence="28"/>
    <dbReference type="EC" id="2.6.1.40" evidence="12"/>
    <dbReference type="EC" id="2.6.1.44" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KX38"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003102"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003139.1">
        <property type="protein sequence ID" value="ENSXCOP00000003102.1"/>
        <property type="gene ID" value="ENSXCOG00000002451.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156125"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047305">
        <property type="term" value="F:(R)-3-amino-2-methylpropionate-pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016223">
        <property type="term" value="F:beta-alanine:pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009436">
        <property type="term" value="P:glyoxylate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019481">
        <property type="term" value="P:L-alanine catabolic process, by transamination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000055">
        <property type="entry name" value="Alanine--glyoxylate aminotransferase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45688">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45688:SF3">
        <property type="entry name" value="ALANINE--GLYOXYLATE AMINOTRANSFERASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 39" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="41" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
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            <dbReference type="ARBA" id="ARBA00013049"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00043669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00043726"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00043749"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00043751"/>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00043758"/>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00043777"/>
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    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00043798"/>
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    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00043825"/>
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    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00043826"/>
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    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00044055"/>
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    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00044257"/>
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    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00044258"/>
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    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00047892"/>
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    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00048264"/>
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    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00048500"/>
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    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00048560"/>
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    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00048760"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00048916"/>
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    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00049480"/>
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    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00058068"/>
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    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="40">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="511" mass="56734" checksum="62731D0D176B428F" modified="2018-12-05" version="1">PVKILQEFSICGCNLIEYIKFINFYFTGASCQKSDLRFSRTDIPEMPSCSFTPEEYKGMSKARMIEIRKQNCNPMTMKVTYYKKPVFIHQGYMQYLWDVDGTRYLDLFAGVATVSVGHCHPKVTAAAEKQLKKLWHTTLIYVYPTLQEYCEKLASYLPDPLKVVFLTNSGSEANDLAMFMARLYTGNFDIITLRGSYHGGSPMAMGLTSNAAYKYPVANGLGCTNTMCPDVFRGPWGGRHCRDSPVQTTRDCSCAQGHCAANEQYIRQLKETLATSVPPRIAAFFAEPIQGVGGAVQYPKNYLKDAFKLVRERGGICIADEVQTGFGRTGSHFWGFQGHDVIPDMVTMAKGIGNGFPMGAVVTTPEIANSFTKGVHFNTFGGNPVACAVASAVLETIIEDGTQKNSLDVGTYLMTELAKLRDKYEIIGDVRGKGLQIGVEMVKDKTSRDPLPPEDFAEIFEDVKDMGVLIGKGGVYGQIFRIKPPMCITKEDADFFLAVFNKSVHNYMEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXC6</accession>
    <name>A0A3B5KXC6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Filamin A, alpha (actin binding protein 280)</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the filamin family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000002685"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002720.1">
        <property type="protein sequence ID" value="ENSXCOP00000002685.1"/>
        <property type="gene ID" value="ENSXCOG00000002131.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153588"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21308">
        <property type="entry name" value="CH_FLNA_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000140">
        <property type="entry name" value="FiLamiN (Actin binding protein) homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000006">
        <property type="entry name" value="Filamin-B isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000092">
        <property type="entry name" value="Filamin-B isoform B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000008">
        <property type="entry name" value="Filamin-B isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000007">
        <property type="entry name" value="Filamin-B isoform C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000154">
        <property type="entry name" value="filamin-B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000102">
        <property type="entry name" value="filamin-B isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000001">
        <property type="entry name" value="Filamin-C isoform b"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000105">
        <property type="entry name" value="filamin-C isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000115">
        <property type="entry name" value="filamin-C isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000126">
        <property type="entry name" value="filamin-C isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000157">
        <property type="entry name" value="filamin-C isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000096">
        <property type="entry name" value="filamin-C isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000118">
        <property type="entry name" value="filamin-C isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000168">
        <property type="entry name" value="filamin-C isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044801">
        <property type="entry name" value="Filamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537:SF5">
        <property type="entry name" value="FILAMIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38537">
        <property type="entry name" value="JITTERBUG, ISOFORM N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="20"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="38"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="11">
        <location>
            <begin position="162"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="272"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="372"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="471"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="568"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="665"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="763"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="866"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="965"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="1089"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="1184"/>
            <end position="1283"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="1284"/>
            <end position="1376"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="1377"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="1473"/>
            <end position="1569"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="1570"/>
            <end position="1665"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="1718"/>
            <end position="1776"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="1776"/>
            <end position="1868"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="1887"/>
            <end position="1914"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="1916"/>
            <end position="2009"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="2013"/>
            <end position="2106"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="2140"/>
            <end position="2234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="2234" mass="239270" checksum="D13B8D8CB810B970" modified="2018-12-05" version="1">MSGSHPRLHQSAAPAPNDKDAEMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIGLLEVLSQKKMFRKYNQRPTFRQMQLENVSVALEFLDRENIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEEADDGKQKTPKQRLLGWIQNKLPELPITNFNRDWQTGRALGALVDSCAPGLCPDWDQWDQTKPVDNAREAMQQADDWLGIPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPVGNVVMKKAMFTVETISAGMGEVLVYVEDPAGHREEAKVTANNDKNRTYSVVYTPKVMGMHKVTVLFAGQHISKSPFEVDIGMAQGNSSKVTAQGPGLEPSGNIANKATYFDVYTAGAGVGEVEVEVMDPAGKKNTVPCTIEDKGNSSYRCTYKPTQEGQHTIFITFAGGQISKSPFTVDVGEACNPSLCRAKGRGLQPKGLRVKETADFKVYTKGAGTGELKVTIKGPKGIEEPCKRKDLGDCVYGFEYYPTTPGTYSITITWGGQHIPRSPIEVKIGSEAGQQLVRAWGPGLEGGIVGKSADFVVEAVGQNAGTLGFSVEGPSQAKIECDDKGDGSCDVRYWPTEPGEYAVHVLCNNEDIQHSPFMAEIVNALGKDFYPDKVKAYGPGLQSSGLAVGKPTEFTVDTKQGGSAPLKIVAQVDGEGNPVDVQVKDNGNGTYTCTYTPRKPVKHTVMVSWGGVNIPESPFRMNIGAGCHPNKVKVSGPGVAKTGLKAFEPTYFTVDCSEAGQGDISIGIKCAPGVVGPAEADIDFDIIRNDNDTFTVKYTPPGAGSYTIMVLFAEHAIPMSPIRVKVDPSHDASKVKAEGPGLSRSGVELNKPTHFTVNTKGAGKASLDCSFSGPTKAEAVKDFEVINNHDNTHTVKYTPVQQGPLGVAVTYGGDHIPKSPFNVAVAPTLDLSKVNVTGLGDKMTVGKDQEVTVKSKGAGGQGKVAAKVTGPSGKPVTCKVSLTLDFFFFFFYSKISEFYTVNLLKPTVEPGLSPETSQVKFIPREAGPYQVELTYDGVPIPGSPYTPTAYPPTDPSKVRCSGPGLERAKVGETGEFVVDCTNAGPAELTIEIISDSGTEAEVHIQDNGDGTYTITYIPLYPGSYTLTIRYGGQDVPNFPARLTVEPAIDASGVRVFGPGVEGKGVFRDATTDFTVDARALTQNGGDHIKTRISSPSGSCPDAVITDHGDGMYAVEYTPYEEGPHSVEVCYDGSPVPKSPFRVAVTEGCDPARVRVHGPGLKSGITNKPNKFMVETRGAGTGGLGLAMEGPSEAKMSCTDNKDGSCSVEYIPYEPGTYNLNITYGGQPVKGSPFSVPVSDTVDSTKVKCQGPGLSSNVRANIPQEFTVDASKAGVAPLQVRVQGPKGVVEPVEVVDNGDQTHTVNYVPTREGPYSINVLYADEEIPRSPYKVKVLPTHDASKVRASGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPEGKPKKAKIRDNHDGTYLVSYVPDMTGRYTILIKYGGDEIPYSPYRIRALPTGDASKCTVTGAGVGPTIQIGEQTVITVDAKAAGKGKVTCSVCTPEGAELDVDVVENEDGTFDIFYTAPQPGEYVICVRFGGEHIPNSPFQVTALEGPSDQLMQQRQMPQYYAQQPWATDRPVGMNGLDVTGLRPFDLVIPFTIQKGEITGEVRMPSGKVAKPDITDNKDGTVTVKYGPTEAGLHEMDIKYDGIHIPGSPLQFFVDYMNSGNVSAYGPGLIHGTVNKPAVFTVDTKDAGEGGLSLAIEGPSKADISCMDNQDGTCTVSYLPVLPGDYSILVKYNDKHIPGSPFSARITGDDSMRMSHLKVGSAADIPLDIGDYEVSIRFNDEHIPDSPFIVPVASPSDDARRLTVASLQESGLKVNQPASFAVSLNGAKGVIDAKVHSPSGALEQCCVTEIDQDKYAVRFIPRENGLYLIDVKFNGSHIPGSPFKIRVGETGQAGDPGMVSAYGPGLEGGTTGRTACEFVVNTSTAGPGALAVTIDGPSKVKMDCVECPEGYKVTYTPMAPGNYLISIKYGGPYHIVGSPFKAKITGSKLVSSHSMHETSSVMVDPVTRAISGSQQATPTHSDASKVTTKGPGLTKAFVGQKNNFCVDCSKAGRNMLLVGVDGPKVPCEEILVKHMGNRVYNVSYQLKEKGEYILVVKWGDEHIPGSPYHLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXP4</accession>
    <name>A0A3B5KXP4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Large ribosomal subunit protein mL38</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">39S ribosomal protein L38, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the phosphatidylethanolamine-binding protein family. Mitochondrion-specific ribosomal protein mL38 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXP4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003327"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003365.1">
        <property type="protein sequence ID" value="ENSXCOP00000003327.1"/>
        <property type="gene ID" value="ENSXCOG00000002629.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00900000141125"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.280.10:FF:000002">
        <property type="entry name" value="39S ribosomal protein L38, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF133">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="334" mass="39488" checksum="BBC4BE90C2189B31" modified="2018-12-05" version="1">MPNDDIDTSNLESLETYRSYNRYFRQAEEAKNKRVWWKTYRKRVDIGLPYCRPSRTKVVKERRQAMSENKRNVELERASRLRTLKIPLDQVQETWEKTGGPFDIKKLADHYGVFRDLFPMAYFLPQVTLRISYSQDTDGQVHYGNQLTPTEAVSAPRIAFDAEENSLWTLLLTSPDEHLLDNEAEYLHWLVGNIPGGAVRAGEELCHYLPPFPARGTGFHRYIYVLFKQEKPISFREDLRPSPCHSLVDRTFKTVDFYRKHQDDMTPAGLAFFQSQWDQSVTNTFHNTLNMKEPVFEFIRPPVYHPPQVKYPHRQPLRYLDRYRDGKEHTYGIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYP9</accession>
    <name>A0A3B5KYP9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peroxisomal 2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing]</fullName>
            <ecNumber evidence="9">1.3.1.124</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">2,4-dienoyl-CoA reductase 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(2E,4Z,7Z,10Z,13Z,16Z,19Z)-docosaheptaenoyl-CoA + NADPH + H(+) = (3E,7Z,10Z,13Z,16Z,19Z)-docosahexaenoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:44920"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:77559"/>
            <dbReference type="ChEBI" id="CHEBI:84791"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a (2E,4E)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:45912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85101"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a (2E,4Z)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:61892"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85099"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer, dimer and oligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2,4-dienoyl-CoA reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.124" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KYP9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003160"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003197.1">
        <property type="protein sequence ID" value="ENSXCOP00000003160.1"/>
        <property type="gene ID" value="ENSXCOG00000002357.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153801"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008670">
        <property type="term" value="F:2,4-dienoyl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05369">
        <property type="entry name" value="TER_DECR_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000477">
        <property type="entry name" value="Peroxisomal 2,4-dienoyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045017">
        <property type="entry name" value="DECR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43296">
        <property type="entry name" value="PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43296:SF2">
        <property type="entry name" value="PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE [(3E)-ENOYL-COA-PRODUCING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="289" mass="30397" checksum="5728B14D41E1CB15" modified="2018-12-05" version="1">MAEHRRTEELPEDVGSDDCLASYTHIYSPDLLKDQVAFITGGGSGIGLRFLIICPVLVEYYLCAAVKLSAASGRRCLPLRIDVRQPDGIAAAVDETLKEFGRLDILINNAAGNFLCPASALSFNAFKTVLEIDTMGTFNTSKVVYEKWFKDHGGNVVNVSATLAYRGQALQVHAGSAKAANDAMTKHLAVEWGPSGVRVNAVAPGPISGTEGYRKLGGPAAEAAGSFQAVPLQRAGNKTEMAHCALFLASRASSYVTGAILVADGGAWLTSPNDVSALLGIAASKRAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZ75</accession>
    <name>A0A3B5KZ75_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nipped-B protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">May play a structural role in chromatin. Involved in sister chromatid cohesion, possibly by facilitating the cohesin complex loading. Transcription factor, which may promote cortical neuron migration during brain development by regulating the transcription of crucial genes in this process.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the SCC2/Nipped-B family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000003857"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003900.1">
        <property type="protein sequence ID" value="ENSXCOP00000003857.1"/>
        <property type="gene ID" value="ENSXCOG00000003016.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010427"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090694">
        <property type="term" value="C:Scc2-Scc4 cohesin loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140587">
        <property type="term" value="F:chromatin loop anchoring activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061775">
        <property type="term" value="F:cohesin loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140588">
        <property type="term" value="P:chromatin looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048565">
        <property type="term" value="P:digestive tract development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035118">
        <property type="term" value="P:embryonic pectoral fin morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048703">
        <property type="term" value="P:embryonic viscerocranium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034087">
        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071169">
        <property type="term" value="P:establishment of protein localization to chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003146">
        <property type="term" value="P:heart jogging"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23958">
        <property type="entry name" value="SCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000225">
        <property type="entry name" value="Nipped-B protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026003">
        <property type="entry name" value="Cohesin_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030513">
        <property type="entry name" value="Dehydrin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024986">
        <property type="entry name" value="Nipped-B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033031">
        <property type="entry name" value="Scc2/Nipped-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704:SF18">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN DELANGIN SCC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12765">
        <property type="entry name" value="Cohesin_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12830">
        <property type="entry name" value="Nipped-B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00315">
        <property type="entry name" value="DEHYDRIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="9 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sister chromatid cohesion C-terminal" evidence="13">
        <location>
            <begin position="2282"/>
            <end position="2462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="341"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="400"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="497"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1052"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1101"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="2488"/>
            <end position="2520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="2634"/>
            <end position="2692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="352"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="400"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="521"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="581"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="596"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="936"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="1106"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1131"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="2673"/>
            <end position="2684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF12830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="2692" mass="307746" checksum="49EA3C061E0C2E88" modified="2018-12-05" version="1">MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTAKSLLYNGRISDEVSSLLVCRDENLVTQLAHSLNQVSTEHIELKDNLGNDEPEGDMPILLQTLLTRNPRIFRDKSMLTFASVLQQPLIQQYKISQNQVHGSPASNYQQSPSGCFTSPQCGSATQFVPQQNSPIPSPYTPQSPADYIQYNPPSYSHQTQQVVSGARTILDSKISEHLSSNSSNRNSKPSLDEDYVNVAQRLGNEVMLGIRPRPPLIMQSPPLEAGTDPDLLLTTADRKKKQRERNKEENENMDKNVFYDIVSSPSKDSARLTLKLSRVKIPDTGRSEELLPTSPTDSDHNAVVLNNDIQSSGSPQDFSHKSVVEEHQQDAAQPITKETGVISGIVFDDSEIDAFAEIERIDSANERERCSKEVQDKDKPLKKRKQDTYPQEPGVETNEGPSLHGGSNVSRLTPKKSSAASNGASRPALMVSIDLKQAGRVIGQPVVVLEQQPLCDDHIRRLKTSADTENRPGIIKKLPDTCQMHISDGHTESNKRKQNNQRESKRNHDSKLDCDKGHSEDRHVEMTRLKQDRHSDFRHKEEKNNSSHRSQVSRPETLKSTSKVDRNSKHEEQKGKNKDKDKERDRQRDKDGDKEKLRDKEISRERDKDKVRDRERNRERDKSIDVAREEDKYQDKVKSKLRDGDKFRDGEEPIEREKFREEDKSRERAKDEDKDLEKLKQKLRDRDKDGEKVRERDKSIDLDKNEDQDVERVKDKVRDKDKIRDKEKKRERDKFRERDKDQERVKDNHRENVRGRDKSIDVVKDGDEDVDRIKDKVKDKNRDREKTGGKEKSREIDKDREKDRDKNRNRDKDENFEVGKEQNKDGKRDRERDKGKDRDRNRDRDRERDREKNKKHKTLPEGDGNRISFEEQTKAENSKLKQDEGRKSAELNDRQRTSSSSLQTTPNKDQRASGDSNMNQAANKRQACEMKMSEFPPFLLGGNSGSLKNFVIPKLKRDGKEGTDKDLRLKGKLIDGWTEPLVRLERVSLVKNLNKKTKPVIVVKRLSIEDVKRIIKETKNAHSSRSRHWASFDKSDREVKQQDHNLIHFMFSERADKRKHSMINKGSKYAEVDSDGSDEKDNGDEELESEKRKKKDHDKSWKFEEKKGSRDHRRSGGFQNSRRGSSGRHRDWSCNDDSDGDSPPPSLNDVARKIKKQKSKMAYDSKMTTEEKMDSSAFKRFANNVDNILESLEDVDLTAEDDEIPQELLLGKQQLNELGSDSTKIKAMGVFNKFSSSKLVKILNMLEKNIQDSVTLSTLMSQDNDSMDEERLWRDLIMERVIKSADACLTALNIMTSPHMPKAVYIEDVIERVLQFTKFHLQNTLYPQYDRSYRADRHGGASHTSKSKRPKSSAHKQKVVVGLYNKVCDIISVISELLEIQLLTDTTILQVSSLGITPFFVENVNELQLCAISVVTAVFSRYEKHRQLILEEIFASLARLPTSKRGLRNFRLNTSDSGGPPLYIQMVSALVLQLIQCVVNLPNERETEEEPKKKVDKDILITNSYEIAMRTAQNFLSVFLKKCGSKQGEDDYRPLFENFVHDLLSAVNKPEWPAAELLLSLLGRLLVHQFSNKQTEMALRVASLDYLGTVASNLRKDSLSCKMDQRTIDRILKETPGSDEVQQLQKALLNFLDENIDADPALLFARKFYIAQWYRDTTVEVEKAMKSQNDDDDPKYRRHSMDSDATGEIVQTVEARKKFLRKIIKTTPSNFSSVKISSDTVDYEDSCLIVRYLASMRPFAQSFDIYLSQILRVLGESAIAVRTKAMKCLSEVVAVDPSILGRVDMQRGVHCRLMDNSTSVREAAVELLGRFVLSRPELIEQYYDMLIERILDTGISVRKRVIKILRDICLELPDFHKITEMCVRMIRRVNDEEGIKKLVNETFQKLWFSPTPSHDEEAMNRKILNITDVVLACKDSGYDWFEQLLQNLLKSEEDASYKPTKKACVQLVDNLVEHILKYEESIADCEEKGINSGRVVACITTLFLVTKIRPILMVKHAMAMQPYLNTKCNTQNDFMVICNVSKILELVVPLIENPSESFLNTIEGDLMKLIIKHGMTVVQHCVSCLGAIVNKVTHNYKFVWACFNRFYGALAKLKTQHQENPSGSTLAANKPTLLRSLFTVGALCRHFDFDQEEFKGANKIIIKDKVLELLLYFTTHEDEEVQIKAIIGLGFQFIMHPELMFAQDVKVLYISLLSEENRAVNLKIHVLKNLQTYLQEEDSRMQEADREWNKQAKQEDLKEMGDISSGMSSSIMQIYLKQVLESFFHSQSTVRHFALNVITLTLSQGLIHPVQCVPYLVAMGTDPEPTMKNKADQQLVEIDKKYSGFIHMKAVAGLKMSYQVQQAINGGKRAAVRGFRHDDSDSALCSHLYSMVRGNRQHRRAFLISLLNLFDDSSKTEVNMLLFIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKEVRVFVYRLDSSSSSSSSSSDEEEEEVRKRKKPGGGDSDSDLGDEEAVMDRLPENTTPLQEFASASQGILLLLVLKQHLKNLYGFSDSKIQKYSPTESAKVYDKAVNRKSKVHFNPRQTLEYLRSDVANRNLSYETKRNIVKQFLDFKVLMEHLDRDEEDEDGGEANANARNKAINSLLKGPKPQNHNHSNHTAPVETDDEESEDEDPPAVRMTIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2W2</accession>
    <name>A0A3B5L2W2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sulfotransferase</fullName>
            <ecNumber evidence="6">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2W2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004201"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004247.1">
        <property type="protein sequence ID" value="ENSXCOP00000004201.1"/>
        <property type="gene ID" value="ENSXCOG00000003299.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163403"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006584">
        <property type="term" value="P:catecholamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000433">
        <property type="entry name" value="Estrogen sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11783">
        <property type="entry name" value="SULFOTRANSFERASE SULT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0128">Catecholamine metabolism</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="7">
        <location>
            <begin position="36"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="311" mass="36129" checksum="60C142077689A2F0" modified="2018-12-05" version="1">MARLDVTEIFHGISFPGHLHTQDSLQHALKFLFQDMDVLIVSYPKSGTTWMQEIVTLISSRGDPHVSQTTPNWTRAPWLEQYYCADLLKSSTSTPRVLTTHLPHDLLGPALQESKARIIYVSRNPKDVLVSFYHFHKMANFLPDAGSFPEFLHHFLEGTLHFGSWFDHIKGWTSQAAALNLLHVTYEEMSTDLHRTINKVSSFLQRPLLEHELNNCVKHCSFSRMKDNKMINYSLIPDEILDHSKGCFMRKGEIGDWRNMFTEEQNRYFDTAFKSKMQDCILKFKFLLEKLYNVTAAGKEILLLLFFHTLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4J6</accession>
    <name>A0A3B5L4J6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L4J6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002719.1">
        <property type="protein sequence ID" value="ENSXCOP00000002684.1"/>
        <property type="gene ID" value="ENSXCOG00000002142.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163520"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099558">
        <property type="term" value="P:maintenance of synapse structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050822">
        <property type="entry name" value="Cerebellin_Synaptic_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923:SF103">
        <property type="entry name" value="CEREBELLIN 20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923">
        <property type="entry name" value="CEREBELLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="C1q" evidence="4">
        <location>
            <begin position="44"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="185" mass="20235" checksum="4DCB7D6CD2539392" modified="2018-12-05" version="1">CSNNQAACGCCLMQKQMERMELVFNVTTKEMSQQLMNSKMTLNNIKNRSAFSVALNNEKTLTCFGPMTEDRRVPYKYIFLNLGDSYNAQTGIFTAPSSGVYSLAATVYSLSPLDLPQATCAQLQVNGVAVAPLIEKNGLDSEDSSTVVIARHLKAGDQVSVSLLKGCNICDDTSHYNTFTGFLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4Y5</accession>
    <name>A0A3B5L4Y5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Anthrax toxin receptor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton and thereby plays an important role in normal extracellular matrix (ECM) homeostasis. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells. May also act as a receptor for PLAU. Upon ligand binding, stimulates the phosphorylation of EGFR and ERK1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with gelatin and type 1 collagen. Interacts with the actin cytoskeleton.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cell projection</location>
            <location evidence="14">Filopodium membrane</location>
            <topology evidence="14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Cell projection</location>
            <location evidence="13">Lamellipodium membrane</location>
            <topology evidence="13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L4Y5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005948.1">
        <property type="protein sequence ID" value="ENSXCOP00000005882.1"/>
        <property type="gene ID" value="ENSXCOG00000004560.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156522"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031527">
        <property type="term" value="C:filopodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031258">
        <property type="term" value="C:lamellipodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01474">
        <property type="entry name" value="vWA_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000017">
        <property type="entry name" value="Anthrax toxin receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008399">
        <property type="entry name" value="Anthrax_toxin_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008400">
        <property type="entry name" value="Anthrax_toxin_rcpt_extracel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16059">
        <property type="entry name" value="ANTHRAX TOXIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16059:SF28">
        <property type="entry name" value="ANTXR CELL ADHESION MOLECULE 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05586">
        <property type="entry name" value="Ant_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05587">
        <property type="entry name" value="Anth_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="338"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="20">
        <location>
            <begin position="87"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="532"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="566"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="610"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00093334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="644" mass="72281" checksum="184D5AF0CA8C4E54" modified="2018-12-05" version="1">MLRRSVFPGCLSTLEHFKRGIFQLENICFYSHSCRGKCKFVIMSQSWCGQAILRVELGILVAVLVGSSMAQIYERREEGSSCYGGFDLYFVLDKSGSVQYYWNEIYYFVDHLAHKFISPQLRMSFIVFSMEGRILMPLTEDREQIRTGLEELRMVHPGGDTFMHRGFQRASEQIYYGTGYGYRTASVIIALTDGELRENQFDLAEREAGRARQLGATVYCVGVKDFNETQLSTIADSKDHVFPVHDGFQALQGVIDSILKRSCIEILAVEPSSICEGENFQVSVRGNGFLHARDVQKVLCSFRINDTVTFMERPLVVRDTYLLCPAPVLERFGMSATLYVSMNNGLSFISSSVTITAITCSDGTFVGIALLILLLLFTVILLWWFWPLCCTMVIHEPPPPVIEDLSDEDGFPKKRWPTVDASYYGGRGVGGIKRMEVRWGDKGSTEEGAKLEKAKNARVVMPTPEYDPPPPRLYYHTRKTISSQKWYSPIKGKLDALYVLLRRGYDRVSVMRPYPGDKGRCINFTRSPSYPPPRYPIYNHPSTPVYTTPHRYQRGPSDDCEDLHLPPSPTSTLPPLPSTPPPSSSSCSSSYTPPPSRDLPPTNGRQNSAPPSPTGSLPPPPQASPPCRAPPPSRPPPRPNHEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L520</accession>
    <name>A0A3B5L520_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">5'-nucleotidase</fullName>
            <ecNumber evidence="3 9">3.1.3.5</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the pyrimidine 5'-nucleotidase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="3 9"/>
    <dbReference type="RefSeq" id="XP_027869006.1">
        <property type="nucleotide sequence ID" value="XM_028013205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L520"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005917"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005983.1">
        <property type="protein sequence ID" value="ENSXCOP00000005917.1"/>
        <property type="gene ID" value="ENSXCOG00000004588.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114142117"/>
    <dbReference type="CTD" id="51251"/>
    <dbReference type="GeneTree" id="ENSGT00390000012959"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07504">
        <property type="entry name" value="HAD_5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.340:FF:000001">
        <property type="entry name" value="Cytosolic 5-nucleotidase 3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000032">
        <property type="entry name" value="Cytosolic 5-nucleotidase 3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.340">
        <property type="entry name" value="Pyrimidine 5'-nucleotidase (UMPH-1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006434">
        <property type="entry name" value="Pyrimidine_nucleotidase_eu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01544">
        <property type="entry name" value="HAD-SF-IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13045">
        <property type="entry name" value="5'-NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13045:SF14">
        <property type="entry name" value="CYTOSOLIC 5'-NUCLEOTIDASE 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05822">
        <property type="entry name" value="UMPH-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01128">
        <property type="entry name" value="C1.4:_5'-Nucleotidase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="285" mass="33057" checksum="60BB88AEB4031E0F" modified="2018-12-05" version="1">MPQFEKPTVHMRDPERVEQIICGLIKGGASKLQVITDFDMTISKFAVNGKRCPTLHNVIDNCKLVTEECRQKLLQLKHKYYPIEIDPHLSMEEKYPFMVEWYFKSHSLLVEQRLEKHKLPEVVRESDLVLRDGYEQFFDRLHQHNVPVFIFSAGLGDVLEEVISQARVYHPNMKVVSNFMDFDDKGVLKGFKGELIHVYNKHEGALRNTDYFKQLRDYSNIILLGDSLGDLTMADGAPNVENMLKVGFLNDKVEEHLDKYMDSYDIVLVKDETLEVPNAILQKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5S3</accession>
    <name>A0A3B5L5S3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Retinoblastoma-like protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">107 kDa retinoblastoma-associated protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">pRb1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. May act as a tumor suppressor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Interacts with AATF. Interacts with KDM5A. Interacts with KMT5B and KMT5C. Interacts with USP4. Interacts with RBBP9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the retinoblastoma protein (RB) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5S3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003114"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003151.1">
        <property type="protein sequence ID" value="ENSXCOP00000003114.1"/>
        <property type="gene ID" value="ENSXCOG00000002462.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183202"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000134">
        <property type="term" value="P:negative regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000035">
        <property type="entry name" value="RB transcriptional corepressor-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.140:FF:000001">
        <property type="entry name" value="Retinoblastoma-like 2, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000082">
        <property type="entry name" value="retinoblastoma-like protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002720">
        <property type="entry name" value="RB_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002719">
        <property type="entry name" value="RB_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028309">
        <property type="entry name" value="RB_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024599">
        <property type="entry name" value="RB_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742">
        <property type="entry name" value="RETINOBLASTOMA-ASSOCIATED PROTEIN RB -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13742:SF20">
        <property type="entry name" value="RETINOBLASTOMA-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11934">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01858">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01857">
        <property type="entry name" value="RB_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01367">
        <property type="entry name" value="DUF3452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01368">
        <property type="entry name" value="RB_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Retinoblastoma-associated protein N-terminal" evidence="17">
        <location>
            <begin position="72"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Retinoblastoma-associated protein A-box" evidence="18">
        <location>
            <begin position="374"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="16">
        <location>
            <begin position="770"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="957"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="965"/>
            <end position="975"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1010" mass="113248" checksum="C3D10215E5B4C150" modified="2018-12-05" version="1">MRGEESSDSESGRMEESSVRRSLETLCQELNMDEQTAAEAMDNFTAIWNTYSLEGEVVHWLACALYAACRKGSTPTVGKGLMEGNCVSLTRILRCAKLSLIQFFSKMRKWADMSNLSQDFRLRMDRLERNFEVSTVIFRKFEPIFRDMFVNPQGCEAPRQPRSRKHRRLPCHITDVFKFCWTLFVYTKGNFRMIGDDLVNSYHLLLCCLDLVFGNALLCANRKELINPGFRGQRLEAPPCVLEKLCELHDGLVVEAKGIKQHYFKPYIHKLFHKQILKGNETLFTEMLDPQNFIDNNKAINKEYEEYVLTAGDFDERVFLGADADEEIGTPRKFVQERIEVETFLSLTLQRGSLAPSTPLTGRVYLKEKDLLFTPVSAATQSVTRLQSMVAGLRMAPSENLLQIFRSCSRDPTDSILARVKALGQTFKEHYTNDSEDTPGSHIGTRNLAEILYHKVLENVLVQETKRLQGRDMSVLLEQDIFHCSLMACCLEVVLFSYSSQRTFPWIISVFKLPPFYFFKVIEVFIRSEERLSRDMVKHLNAIEEQVLESRAWASDSALWSALEAAGGKVPTVEEVGSGKFAERSVSCVLTFVTVSTDSRRFCLLPEVPPSPVSVHDRYSSPAAGSAKRRLFGDDPPAPPAGLNIAISPTPSPVKRLTFGTSQKNGGQSTVLSIPLQGGSGSGDVTEVQRNQNLLYVLMCAGERAFALVPVQPCDSSGVVTAHFLLTSSPGRVAVATATSSTDPQAGTGRPRRTGSLALFFRKVYHLSSVRLRDLCLKLDVSSDLRQKIWTCFEHVVVQCSDLMKDRHLDQLLLCCVYIIAKITKETITFQDIMKCYRSQPQASSHVSNSHRSASAAAAQPALTLCVCVCQPAGRHPDQSEEEERGDLIQFYNMVFVLKMKSFAMRYADAPPLSPFPSVRAQPLSPRRVSQRHSLYVSPHKNSAGSLTPNSFTYRINSSPSKANTRTHTHTHTHTHTCDSPNKRACPENGSGPDVLLKRLQDVVSERQSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L634</accession>
    <name>A0A3B5L634_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the recoverin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L634"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007773"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007871.1">
        <property type="protein sequence ID" value="ENSXCOP00000007773.1"/>
        <property type="gene ID" value="ENSXCOG00000005969.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158048"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000009">
        <property type="entry name" value="Visinin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF89">
        <property type="entry name" value="KV CHANNEL INTERACTING PROTEIN 1 B ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="58"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="94"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="142"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="188" mass="22076" checksum="077BBFB3884FAA15" modified="2018-12-05" version="1">MTMVCHRPEGLEQLEAQTNFSKQELQILYRGFKNECPSGVVNEETFKHIYAQFFPHGDASMYAHYLFNAFDTTNNGSIKFKDFVVGLSILLRGTLREKLEWTFHLYDINRDGYINREEMTEIVRAIYDMMGKYTYPALKGDVPQQHVDAFFQKMDKNKDGVVTMEEFIIACQEDENMMRSMQLFENVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6P5</accession>
    <name>A0A3B5L6P5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WxxW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6P5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005508.1">
        <property type="protein sequence ID" value="ENSXCOP00000005446.1"/>
        <property type="gene ID" value="ENSXCOG00000004250.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008152"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039675">
        <property type="entry name" value="CILP1/CILP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025155">
        <property type="entry name" value="WxxW_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15031:SF4">
        <property type="entry name" value="CARTILAGE INTERMEDIATE LAYER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15031">
        <property type="entry name" value="CARTILAGE INTERMEDIATE LAYER PROTEIN CLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13330">
        <property type="entry name" value="Mucin2_WxxW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="WxxW" evidence="5">
        <location>
            <begin position="6"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="WxxW" evidence="5">
        <location>
            <begin position="89"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="180" mass="20772" checksum="0654CB7FBE8649E2" modified="2018-12-05" version="1">NLKEHNPYGDWETLSSLRRENPGKICPNPKSIEARTLSGRNAEDTGEKIYKYDTTSGFVCRMKDQRDRKCHDYKVRFSCSFDTCENVCWTKWYNRDHPSGSGDWEHLSALRKEKHGGDLCADPVYVEAVTVEDEIPALKTGQKSHYYSPGKGFVCHNKDQRFDKCRNYKVRFGCSCRSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L712</accession>
    <name>A0A3B5L712_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SUEL-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L712"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003608.1">
        <property type="protein sequence ID" value="ENSXCOP00000003569.1"/>
        <property type="gene ID" value="ENSXCOG00000002816.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154285"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22833">
        <property type="entry name" value="Gal_Rha_Lectin_CSL1-2_RBL_SML_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22835">
        <property type="entry name" value="Gal_Rha_Lectin_SML_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.740:FF:000001">
        <property type="entry name" value="Adhesion G protein-coupled receptor L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.740">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000922">
        <property type="entry name" value="Lectin_gal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043159">
        <property type="entry name" value="Lectin_gal-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46780">
        <property type="entry name" value="PROTEIN EVA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02140">
        <property type="entry name" value="SUEL_Lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50228">
        <property type="entry name" value="SUEL_LECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SUEL-type lectin" evidence="3">
        <location>
            <begin position="27"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="SUEL-type lectin" evidence="3">
        <location>
            <begin position="117"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="208" mass="23046" checksum="677777D93BF47D7D" modified="2018-12-05" version="1">YTLFNLFFTVAVTEKATTCDDSDNIQHLSCDNGVISVQSALYGRADRQICSEGKSPQQLANTQCSQDGAVDIIKNRCDGKRVCEFNTGDVRSSDPCVGIAKYLETNYTCLPANRLVTCEHSNSHLYCDYGQVIFVYGADYGRRDRTTCSYRRPASQTENVYCSNPTNIVAQTCNGKNSCTIRAHNSVFGDPCTGTFKYLEVAYTCECE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L782</accession>
    <name>A0A3B5L782_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Galactose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="13">2.7.7.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>alpha-D-galactose 1-phosphate + UDP-alpha-D-glucose = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:13989"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.7.7.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.12" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L782"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006682"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006764.1">
        <property type="protein sequence ID" value="ENSXCOP00000006682.1"/>
        <property type="gene ID" value="ENSXCOG00000005175.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016188"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008108">
        <property type="term" value="F:UDP-glucose:hexose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00608">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000002">
        <property type="entry name" value="Galactose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001937">
        <property type="entry name" value="GalP_UDPtransf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019779">
        <property type="entry name" value="GalP_UDPtransf1_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005850">
        <property type="entry name" value="GalP_Utransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005849">
        <property type="entry name" value="GalP_Utransf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00209">
        <property type="entry name" value="galT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11943">
        <property type="entry name" value="GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11943:SF1">
        <property type="entry name" value="GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02744">
        <property type="entry name" value="GalP_UDP_tr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01087">
        <property type="entry name" value="GalP_UDP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000808">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00117">
        <property type="entry name" value="GAL_P_UDP_TRANSF_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9 13" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Galactose-1-phosphate uridyl transferase N-terminal" evidence="15">
        <location>
            <begin position="10"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Galactose-1-phosphate uridyl transferase C-terminal" evidence="16">
        <location>
            <begin position="235"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-UMP-histidine intermediate" evidence="11">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="29"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <begin position="78"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <begin position="160"/>
            <end position="162"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="296"/>
            <end position="297"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="301"/>
            <end position="302"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001107"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
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            <dbReference type="RuleBase" id="RU000506"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006682.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    </evidence>
    <sequence length="339" mass="38913" checksum="7F6EF5568C9A1701" modified="2018-12-05" version="1">LSVLLLPPEHQHLRYNPLKDSWVLVSAHRMKRPWAGQVEKPPEELIPRYDPRNPLCPGNTRANGEVNPSYDSTFVFENDFPALQPDAPDPGSDQHPLFQSKAARGVCKVMCFHPWSDVTLPLMKKEEIIKVIDKWAEIVEELGATYPWVQIFENKGAMMGCSNPHPHCQVWASSFLPNEPAVSDRCQRAYYQKNGEPLLVQYAKQEAEKKVHYVLIKIKFPIAFLLFLYLYCCSSGLAAIMKQLLTKYDNLFEVSFPYSMGWHGAPTGPHLKEDNSHWQLHAHYYPPLLRSATVKKFMVGYEMLAQEQRDLTPEQVTHFALYTDQCGTRFWLTSAIAHL</sequence>
</entry>
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    <accession>A0A3B5L9G5</accession>
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        <submittedName>
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    </protein>
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            <taxon>Neoteleostei</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="63"/>
            <end position="101"/>
        </location>
    </feature>
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            <dbReference type="Ensembl" id="ENSXCOP00000007457.1"/>
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        <source>
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    <sequence length="125" mass="13640" checksum="5346A76496360C73" modified="2018-12-05" version="1">SSSVIRAVMERVLRLSHAELLKFVFGTCALQALDLVDQRSVTCLSSPSGRKAFQVTGGSGRLYTCFLSCHYCPCPAFTYTVLRRNQGLLCKHILAVYLCQAMAVTQQESVSDQQMSAVLSGTESP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAE1</accession>
    <name>A0A3B5LAE1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Vertebrata</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="entry name" value="LEUCOKININ RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45695">
        <property type="entry name" value="LEUCOKININ RECEPTOR-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="42"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="399" mass="44649" checksum="A2373F4E793F7537" modified="2018-12-05" version="1">METPGLETNNSGLNQISKEDRALIKTLVPFLDGLILLTGLIGQTLVMIILTGRRKKERQPPHGTDTLLLALCAADLLMLLCQPFHTSAIIVGFWPFGSFHCKAISFLGVACSTASVFTLAALAATRYLVVVHPTWTYRARMNRRLKVTVALLWIPASALAAPQFAFRTITFSNTVYCFAFLSDFSRLVYSIALFVIGFALPLSIITVMYSKIYCFLRHTRQVGNAPQLERYQRQVTHTSALLVLVFTLLWLPSYILMFSIIRGSLASSPGFNTFALLARLLSSSVAVVNPVLYGFMSQKFRRELVELGRESWTRCRSCLISCPRLMSRHTVRPFDQIGESTAVRLHLTSSTLYWLTKCELVTVGIEEETSVAFLTTQNLLSTTILRSEPSSLICWMLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAG1</accession>
    <name>A0A3B5LAG1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibroblast growth factor</fullName>
            <shortName evidence="2">FGF</shortName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAG1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007475"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007571.1">
        <property type="protein sequence ID" value="ENSXCOP00000007475.1"/>
        <property type="gene ID" value="ENSXCOG00000005768.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157821"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="68" mass="7745" checksum="FC46BA9A54934D32" modified="2018-12-05" version="1">MNSRGRLYGTTVFHDECKFRESLLANNYNAYESAAHRGCFIALSKHGRVKRGNRATTAMTVTHFLPRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LB03</accession>
    <name>A0A3B5LB03_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LB03"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008105.1">
        <property type="protein sequence ID" value="ENSXCOP00000008007.1"/>
        <property type="gene ID" value="ENSXCOG00000006152.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234575"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001872">
        <property type="term" value="F:(1-&gt;3)-beta-D-glucan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071226">
        <property type="term" value="P:cellular response to molecule of fungal origin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042808">
        <property type="entry name" value="CLEC7A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47218">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 7 MEMBER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47218:SF2">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="82"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="181" mass="20807" checksum="761A3C5B2F0D67AB" modified="2018-12-05" version="1">MSQPDLEKLIHAFIFSRIDYCNSVFTGLSNKSIKQLQLIQNAAAGVLTKTRKIEHITPVLNLCSDLFEGETFLKCEAGWEKHGGNCYYFSTSKSSWINSRRSCVDLGSDLVKIDSREEQLFLEIRLRGLMMKDTDKFWIGLTDSVEEGKWFWVDGSPLDERLQTTNENILVYFENPSHNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBI0</accession>
    <name>A0A3B5LBI0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LRRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBI0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005229"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005290.1">
        <property type="protein sequence ID" value="ENSXCOP00000005229.1"/>
        <property type="gene ID" value="ENSXCOG00000004093.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165355"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001164">
        <property type="entry name" value="GH01279p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF96">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017226332" description="LRRCT domain-containing protein" evidence="4">
        <location>
            <begin position="21"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="5">
        <location>
            <begin position="271"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="327" mass="36955" checksum="BE4126669F924476" modified="2018-12-05" version="1">MRNSWQIFLDIFSCPALCKCYPTSMEVVCNEVPLTEYPSAGLPENTTMLTIQLTNITSISEHHLKSTPLLKELHLYGNQLQSLSSHLLRGVPQLHTLDLTGNELSDLPVDVFSDAPLRNLVLKNNQIKRADPKWLPHNSSLTWLDLSENRLTEIPVGLFQRLPHLQNIDLSNNRLEKIPPNSLDHLTKLERLNLQNNKLDTLDASVFQFTHNLTSLFLSQNKLAKLPQNLFQGLNRLTHLSLDDNQLSRISKGFLDLLSSLEEGGLDLSGNPLLCDREVEYLWQWLQRNNKKVLFADNITCALPESLAGRSVISLTENDLNIGSQPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBN7</accession>
    <name>A0A3B5LBN7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone deacetylase 2</fullName>
            <shortName evidence="12">HD2</shortName>
            <ecNumber evidence="12">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">HDAC2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6)-(2E)-butenoyl-L-lysyl-[protein] + H2O = (2E)-2-butenoate + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:69172"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13707"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:35899"/>
            <dbReference type="ChEBI" id="CHEBI:137954"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:69173"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:58197"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(6)-acetyl-L-lysyl-[protein] + H2O = L-lysyl-[protein] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58108"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:58109"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBN7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008257"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008355.1">
        <property type="protein sequence ID" value="ENSXCOP00000008257.1"/>
        <property type="gene ID" value="ENSXCOG00000006334.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155725"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016581">
        <property type="term" value="C:NuRD complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160008">
        <property type="term" value="F:protein decrotonylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000003">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF46">
        <property type="entry name" value="HISTONE DEACETYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 12" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="17">
        <location>
            <begin position="30"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="391"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="391"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="403"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="419"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="436"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="446"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="479" mass="54384" checksum="D2C14BD209755DCC" modified="2018-12-05" version="1">MAYTTAGGTKKKVCYYYDGDIGNYYYGQGHPMKPHRIRMTHNLLLNYGLYRKMEVYRPHKATADEMTKYHSDDYIKFLRSIRPDNMSEFSKQMQRFNVGEDCPVFDGLFEFCQLSAGGSAAGSVKLNRQQTDIAVNWAGGLHHAKKSEASGFCYVNDIVLAILELLKYHQRVLYIDIDIHHGDGVEEAFYTTDRVMTVSFHKYGEYFPGTGDLRLGAAGKGKYYAVNFPLRDGIDDESYGQIFKPVMAKVMEMYQPSAVVLQCGADSLSGDRLGCFNLTIRGHAKCVEYIKSFNLPLLMLGGGGYTIRNVARCWTYETAVALDTDIPDELPYNDYFEYFGPDFKLHISPSNMTNQNTMEYMDKIKQRLFENLRMLPHAPGVQMQAIPEDAVPEDTVDEDTEDPDKRLSIRATDKRIACDEEFSDSEDEGQGGRRNTANHKKSAKRPKVDEEKEREEKKADVKEEDKTKDSGSEKAESKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCF9</accession>
    <name>A0A3B5LCF9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphoethanolamine/phosphocholine phosphatase 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCF9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008225"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008323.1">
        <property type="protein sequence ID" value="ENSXCOP00000008225.1"/>
        <property type="gene ID" value="ENSXCOG00000006312.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007741"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006384">
        <property type="entry name" value="HAD_hydro_PyrdxlP_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016965">
        <property type="entry name" value="Pase_PHOSPHO-typ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01489">
        <property type="entry name" value="DKMTPPase-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20889">
        <property type="entry name" value="PHOSPHATASE, ORPHAN 1, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20889:SF2">
        <property type="entry name" value="PHOSPHOETHANOLAMINE_PHOSPHOCHOLINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06888">
        <property type="entry name" value="Put_Phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031051">
        <property type="entry name" value="PyrdxlP_Pase_PHOSPHO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="29"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="31"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR031051-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR031051-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR031051-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="30337" checksum="72D33B81474579EC" modified="2018-12-05" version="1">FFHAPPPEGPPKMSSSSTTNNSRFLMFFDFDETIISENSDNTVVNLLPNQQLPSWLLDSYREGHYHEFSGKITAYLADQGVTKDALLSAVQKIPPNPGMLDLFRFLQSHQQDFELVVVSDANTIFIETWLEHNRLGHLFRRVFTNPSHFDDSGRLVLHPFHSHSCPRCPDNMCKQAILHEYVAVREKERGGVGFQRVFYVGDGSNDVCPTRALGPQDTAFARRDYPMHTLMMEMQQSESTKIKANVVPWVTGADIEDYLGNIMKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCK5</accession>
    <name>A0A3B5LCK5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCK5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008577"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008683.1">
        <property type="protein sequence ID" value="ENSXCOP00000008577.1"/>
        <property type="gene ID" value="ENSXCOG00000006568.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154669"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="174" mass="20113" checksum="73032A9F02AA474B" modified="2018-12-05" version="1">MAPSQWEPLTDKVHPQILELIRQDKRTIRKIPIEPERANLTPKEEEALEELQNRHAIIIKPADKGSTVVIMDKGDYALEAMRQLGDTEVYKPLEEPIYPNTQVQINTLLKQLHKKKHINRKQLLSLISTDPPRPRYFYLLPKIHKKPAEWTKPFHIPKGRPIISDCGSESYGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDX8</accession>
    <name>A0A3B5LDX8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pro-opiomelanocortin/corticotropin ACTH central region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Stimulates the adrenal glands to release cortisol.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the POMC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDX8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006074"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006144.1">
        <property type="protein sequence ID" value="ENSXCOP00000006074.1"/>
        <property type="gene ID" value="ENSXCOG00000004702.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016811"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:neuropeptide signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Mcrtin_ACTH_cent"/>
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    <dbReference type="InterPro" id="IPR001941">
        <property type="entry name" value="PMOC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050878">
        <property type="entry name" value="POMC-derived_peptides"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11416">
        <property type="entry name" value="PRO-OPIOMELANOCORTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11416:SF7">
        <property type="entry name" value="PRO-OPIOMELANOCORTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00976">
        <property type="entry name" value="ACTH_domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00383">
        <property type="entry name" value="MELANOCORTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01363">
        <property type="entry name" value="ACTH_domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="6" id="KW-0372">Hormone</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017346281" description="Pro-opiomelanocortin/corticotropin ACTH central region domain-containing protein" evidence="8">
        <location>
            <begin position="28"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Pro-opiomelanocortin/corticotropin ACTH central region" evidence="9">
        <location>
            <begin position="114"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Pro-opiomelanocortin/corticotropin ACTH central region" evidence="9">
        <location>
            <begin position="168"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="222" mass="25514" checksum="C1489649D7AA7E88" modified="2018-12-05" version="1">SELEKKMVCQCWLFMVLMGFMCSSGSGSVCLESSTCNDLSNKDIIQDCLHLCTSAIEPETKPPRVTDQQENDDFDDPEESHTGDLLLSIILSTLASEGKVPEGDLKTHDDQRRSYAMEHFRWGKPLGRKRRPVKVFPSAVDEEVFPLRARRHLSWNKAESKGGRKAETYRMSHFRWGSPLASKQNNGFVKLWEEKPREKLSKLFKNILVKDLYVIHIRKDDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LER8</accession>
    <name>A0A3B5LER8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DSCAM/DSCAML C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LER8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008181"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008279.1">
        <property type="protein sequence ID" value="ENSXCOP00000008181.1"/>
        <property type="gene ID" value="ENSXCOG00000006282.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155354"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070593">
        <property type="term" value="P:dendrite self-avoidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056754">
        <property type="entry name" value="DSCAM/DSCAML_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075">
        <property type="entry name" value="BASIGIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075:SF72">
        <property type="entry name" value="CELL ADHESION MOLECULE DSCAML1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25059">
        <property type="entry name" value="FN3_DSCAM-DSCAML_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="DSCAM/DSCAML C-terminal" evidence="11">
        <location>
            <begin position="2"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="180"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="304"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="391"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="239"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="394"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="450" mass="49311" checksum="9A9933E7D6CCAC4E" modified="2018-12-05" version="1">MLDFRPKGTWAWQSLKANSTNQLFLSELREATWYELKMKACNSAGCGNQTSQFATTDYDGSTIPPIKSARGEGDDVKKLFSIGSPVILVTLGVALLFIIRKKRKEKRLKRLRDTPVKGPPQGPRLHIDIPRVQLLIEDKEEDKAAVPVTDTEFSQSVNPQNFCTGVSLHHQALIQNSGPLIDMSDIRPGTNPVSRKSIKSAHSSRNRYSSQWTLSRPSQSTASARTLTSDWRTMGSHGITATESDSYSASLSQDTDKGRNSMVSTESASSTYEELARAYEHAKLEEHLQHAKFTITECFISDSSSDQMTTGTNDPADSMTSLSTPSEPGICRFTASPPKPQDYDRGKNVAVPIPHRANKSKWSNLCRCFMVLCRSFHTPRTFPHVVSSQTLTMPSSNNAATASTSSTSSNPTNSNKVGGSRDSLLESSSSALGRLQKQSVGAYSKSYTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFK4</accession>
    <name>A0A3B5LFK4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ferric oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the STEAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFK4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009627"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009741.1">
        <property type="protein sequence ID" value="ENSXCOP00000009627.1"/>
        <property type="gene ID" value="ENSXCOG00000007335.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008042"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008823">
        <property type="term" value="F:cupric reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051267">
        <property type="entry name" value="STEAP_metalloreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239">
        <property type="entry name" value="DUDULIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239:SF6">
        <property type="entry name" value="METALLOREDUCTASE STEAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Ferric oxidoreductase" evidence="7">
        <location>
            <begin position="217"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="448" mass="50841" checksum="9AADCFF39C43E393" modified="2018-12-05" version="1">MANINWHSARGHKYSIVILYPQNINGNMFILPVKRYPPRSHISSPSCSVSARRKTQNSLVGCFLGKVLVDVSNNRRLNQYPESNAEYLASLLPDSVVVKGFNIISAWAMQQSYPKDASTQVFICSDSIEARQLIMELARQLNFQPVDMGPLSWSRYIENIPVQLFPGWKGPVLAAVALSIFFFGYSFVRDIIHPYVKHKQSDFYKIPIEIVNHTLPTVAITLLALVYLAGQLAAAHQLYYGTKYKQFPHWLESWLQSRKQLGLISFFLAAVHILYSLSLPLRKSERYLLLNTAYQQIHANIENSWNEEEVWRVEMYVSFGIMSFGLMSLLAITSIPSISNSLNWREFSFIQTTLGYIALLIATIHGLLFGWRRALDEDAYRFYLPPSFVVSLALPVCVILGKVLMLLPCVGHKLHQIRKGLDTSPCKARRLEPVGSAANISPERVTIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFY1</accession>
    <name>A0A3B5LFY1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFY1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008586"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008692.1">
        <property type="protein sequence ID" value="ENSXCOP00000008586.1"/>
        <property type="gene ID" value="ENSXCOG00000006575.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000263720"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032549">
        <property type="entry name" value="DUF4939"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032567">
        <property type="entry name" value="RTL1-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503">
        <property type="entry name" value="LDOC1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503:SF22">
        <property type="entry name" value="TRANSPOSON TY3-I GAG POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16297">
        <property type="entry name" value="DUF4939"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="3">
        <location>
            <begin position="192"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="41"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="254" mass="27790" checksum="5F36262227BF43AF" modified="2018-12-05" version="1">MSEPSGHVDSAAQLRTALTEQGVLLGRHNNEISVKIKLSAAGTGTPAASPAPVTTSPSPSFSEVISPPPEPFSGEQGKCRGFLLQCALVFGRSPQSFRDDASKISYVVGLLRGKALKWAEAVINESSVHRYSYDDFIESFKQVFDMAASREDASKRLWELRQGNRPPVHSSPLCPPPDPPLPSRLVDGHPAFSVRRILAVHPRGRGRQYLVDWEGYGPEHRSWIPRSFILDPDLISSFERSSAPTSSCPPRGVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LG03</accession>
    <name>A0A3B5LG03_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytosolic carboxypeptidase-like protein 5</fullName>
            <ecNumber evidence="19">3.4.17.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">ATP/GTP-binding protein-like 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Protein deglutamylase CCP5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(L-glutamyl)(n+1)-gamma-L-glutamyl-L-glutamyl-[protein] + H2O = (L-glutamyl)(n)-gamma-L-glutamyl-L-glutamyl-[protein] + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:60004"/>
            <dbReference type="Rhea" id="RHEA-COMP:15519"/>
            <dbReference type="Rhea" id="RHEA-COMP:15675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:143623"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:60005"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>C-terminal L-alpha-aminoacyl-L-glutamyl-L-glutamyl-[tubulin] + H2O = C-terminal L-alpha-aminoacyl-L-glutamyl-[tubulin] + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:63792"/>
            <dbReference type="Rhea" id="RHEA-COMP:16435"/>
            <dbReference type="Rhea" id="RHEA-COMP:16436"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:149555"/>
            <dbReference type="ChEBI" id="CHEBI:149556"/>
            <dbReference type="EC" id="3.4.17.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:63793"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>C-terminal L-alpha-aminoacyl-L-glutamyl-[tubulin] + H2O = C-terminal L-alpha-aminoacyl-[tubulin] + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:63796"/>
            <dbReference type="Rhea" id="RHEA-COMP:16436"/>
            <dbReference type="Rhea" id="RHEA-COMP:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:90782"/>
            <dbReference type="ChEBI" id="CHEBI:149556"/>
            <dbReference type="EC" id="3.4.17.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:63797"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>gamma-L-glutamyl-L-glutamyl-[protein] + H2O = L-glutamyl-[protein] + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:60152"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15517"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:143622"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:60153"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 24">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.24" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LG03"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011068"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011195.1">
        <property type="protein sequence ID" value="ENSXCOP00000011068.1"/>
        <property type="gene ID" value="ENSXCOG00000008362.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158032"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="C:midbody"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:metallocarboxypeptidase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Zn peptidases"/>
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        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE-LIKE PROTEIN 5"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Zn-dependent exopeptidases"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="157"/>
            <end position="588"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
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            <end position="369"/>
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    <feature type="region of interest" description="Disordered" evidence="25">
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            <begin position="630"/>
            <end position="715"/>
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            <end position="658"/>
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            <begin position="664"/>
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        <location>
            <position position="534"/>
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    <sequence length="731" mass="81706" checksum="72E9534C35A472F8" modified="2018-12-05" version="1">MEVRFGNIVFSSRFDSGNLARVEKVEKGNSSPVSDGAQVGSSPSGLHLTPDYEFNVWTQPDCAGTEYENGNRSWFYFSVRGAMPGRLLKINVMNMNNQRKLYSQGMAPFVRTLPVKNRWERIRDRPTVETVNNQFILSFTHRLLDVRGSTTYFSFCYPFSYSECQEMLQQLDESYPNAAHAPGTVYYHRELLCNSLDGNRVDLLTVTNCAGMWDERETRLPKLFPDTNTPRPHRFPDKRVFFLSSRVHPGETPSSFVFNGFLNFILRRDDPRAHTLRSMFVFKLIPILNPDGVVRGHYRTDSRGVNLNRQYLNPNPELHPSIYAAKALLLYHHTHNRLHTTQPTAHSMTQPTPLNNKPANQHQSPPLTPLEISLNQRNEEKGGSSPPPAEVSMVMEENSWSPAELGKGDQNCSADSLEKVASGTGDRADPEEKQSKSVPPQEGGVAYYVDLHGHASKRGCFMYGNNLPDENQQVENMLYPRLIAVNSAHFDFLGCNFSEKNMYARDKRDGQSKEGSGRVAVHKAIGVLHSYTLECNYNTGKTMNSMPPACHDNGRATPPPPSSFPPKYTPEIFEQVGRAVAISALDMAESNPWPRLVLSEHSCLTNLRAWILKHVRNTKGLNTQIHALPKVQSSGSKVSPPKSFNNFLSGSTSENTNGRVRYNSQSSSSSKTPSPKIQSSPSFTFGCTPPPRHHTHHYSTHAGGRGGNRTLGPVKGKLMLPRRVTGGDFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGR6</accession>
    <name>A0A3B5LGR6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Aristaless related homeobox a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family. Bicoid subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027861532.1">
        <property type="nucleotide sequence ID" value="XM_028005731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGR6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008866"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008972.1">
        <property type="protein sequence ID" value="ENSXCOP00000008866.1"/>
        <property type="gene ID" value="ENSXCOG00000006757.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114137153"/>
    <dbReference type="KEGG" id="xco:114137153"/>
    <dbReference type="CTD" id="30657"/>
    <dbReference type="GeneTree" id="ENSGT00940000160633"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:neuron development"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:pancreatic A cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021539">
        <property type="term" value="P:subthalamus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021537">
        <property type="term" value="P:telencephalon development"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HD"/>
    </dbReference>
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        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
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        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050649">
        <property type="entry name" value="Paired_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329:SF337">
        <property type="entry name" value="ARISTALESS RELATED HOMEOBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="222"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="11">
        <location>
            <begin position="435"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="224"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="116"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="400"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="129"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="418"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="467" mass="50044" checksum="7D65039386100E73" modified="2018-12-05" version="1">MSAQFEEDILDRGERKSSKSPTLLSSYCIDSILGRRSPCKVRLIGAQSLTGLPGRGEFDKTTDGPTKEPLSLGADLHLPPKLRRLYGPGGKYLHDHTEKLERERLLLEQASESLKISQAPQVSISRSKSYRENASLHRGEGEQSPGEASEDGALGLVELGPLKFEEDGGKEEEGSCADAAALSPKDEEREEILNAGDGDVRDGEDSVCLSAGSDSEEGMLKRKQRRYRTTFTSYQLEELERAFQKTHYPDVFTREELAMRLDLTEARVQVWFQNRRAKWRKREKAGVQAHPSGLPFPGPLAAGHPLSHYLEGGPFPPHPHPALESAWTAAAAAAAAFPGLAPPHNSSAPPLATPLGLGTFLGTAAMFRHPAFIGPTFGRLFSSMGPLTSASTAAALLRQPAPPVEKPSHAGSVLPDPSSASSSSSSSAAADRRASSIAALRLKAKEHSAQLTQLNILPAGAAGKEVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHQ5</accession>
    <name>A0A3B5LHQ5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Kelch domain containing 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">KLHDC4</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHQ5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010135"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010254.1">
        <property type="protein sequence ID" value="ENSXCOP00000010135.1"/>
        <property type="gene ID" value="ENSXCOG00000007691.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003374"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052588">
        <property type="entry name" value="Kelch_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46063">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46063:SF1">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="350"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="487" mass="54278" checksum="53EB230A2A94D144" modified="2018-12-05" version="1">MGKKGKKEKKGKGADKTAAKMEKKVSKRSKREEEDLEALIAEFQNLDAKKTQVVETTCPPPAPRLNASLSAHPEKDELILFGGEFFNGKKTYLYNDLFFYNIKKNSWVKSDIPNPPPPRCSHQAVVVPQGGGQLWVFGGEFASPNGEQFYHYKDLWVLHLATHTWENIKAAGGPSGRSGHRMVLSKRQLLVFGGFHESTRDFIYYNDVHSFSLDTFSWSRLTPSGSAPSPRSACQMTATPDGAGVIIYGGYSKVRVKKDVEKGSIHSDMFLLKRDEKWSWSRLSPSGSKPPPRSGFSLAVGPAGRAVLFGGVCDEEEEESLEGDFYNDLYLYDTVKNRWFPGLLRGTKSEKKKRRRGKKAEDEGAERKDEEEEETARGPTEVIKEVVTEDGTVMTIKEVIPGSASAPTAEPCPRSSAMATVRQGKLYLYGGMFEVGDRQFTLSDFYCLDLHKMDQWEVLVEMDPSERTNQADAGLIPAEAARLHGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHZ9</accession>
    <name>A0A3B5LHZ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CBM20 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHZ9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010235"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010358.1">
        <property type="protein sequence ID" value="ENSXCOP00000010235.1"/>
        <property type="gene ID" value="ENSXCOG00000007767.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000170918"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001070">
        <property type="term" value="F:starch binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05814">
        <property type="entry name" value="CBM20_Prei4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000752">
        <property type="entry name" value="Putative glycerophosphocholine phosphodiesterase GPCPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013784">
        <property type="entry name" value="Carb-bd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002044">
        <property type="entry name" value="CBM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034839">
        <property type="entry name" value="CBM20_GPCPD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00686">
        <property type="entry name" value="CBM_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01065">
        <property type="entry name" value="CBM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49452">
        <property type="entry name" value="Starch-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51166">
        <property type="entry name" value="CBM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM20" evidence="2">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="156" mass="17535" checksum="9C7C147A22A0A19E" modified="2018-12-05" version="1">METSQVNLTIRGETTPGEVIAVVGSCEALGNWSYQKAVTLHPIGDERHTWAVTISVPRGVVSTYRYFKGFFLKSKSAGGPCQVIVNLWETHHQPRTMSPTVAQQNIDDGEFGFHNGVKCVDSGWLTCQADIRLRLHYSKRPPVSITKKKFKKSRFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LI20</accession>
    <name>A0A3B5LI20_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphodiesterase</fullName>
            <ecNumber evidence="10">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.4.53"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:25278"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LI20"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007499"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007595.1">
        <property type="protein sequence ID" value="ENSXCOP00000007499.1"/>
        <property type="gene ID" value="ENSXCOG00000005788.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164492"/>
    <dbReference type="UniPathway" id="UPA00762">
        <property type="reaction ID" value="UER00747"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006198">
        <property type="term" value="P:cAMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000001">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040844">
        <property type="entry name" value="PDE4_UCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18100">
        <property type="entry name" value="PDE4_UCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0114">cAMP</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="12">
        <location>
            <begin position="84"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="408"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="427"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="452"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="509"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="160"/>
            <end position="164"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="557" mass="62420" checksum="C3657DB31412F7FC" modified="2018-12-05" version="1">MASNKFKRMLNRELTQLSETSRSGNQVSEYIANTFLEKQHDLEILSPPAKEKEKKKRPMSQISGVKKATQSPSLAPACIPRFGVNTPHESMLAKEIEDLNRWGMDIFKLAEYSGNRPLTVMMYSIFQERDLLKTFKVPIDTFLTFMMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEIMAALFASAIHDVDHPGVTNQFLINTSSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLGKKQKDSLRKMVIDMVLATDMSKHMNFLADMKTMVETKKVTSLGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLELYRKWTDRIMVEFFTQGDRERDKGMEISPMCDKHNASMEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSMIPRSPSPTSPDEPHAEVGPAAGTPGSGGAFPSSGGDKFQFELTLEEEEEDEEEAASDLESPLEEESQTSGDRRHDSSSPSLSPDPRGGGSNRYRPPSPHPSRTLSLASMSVRSPHPHRTLGSPGPEASDRNRELSQEGDGVACLPMGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIZ1</accession>
    <name>A0A3B5LIZ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIZ1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007839"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007937.1">
        <property type="protein sequence ID" value="ENSXCOP00000007839.1"/>
        <property type="gene ID" value="ENSXCOG00000006014.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158658"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030055">
        <property type="term" value="C:cell-substrate junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031589">
        <property type="term" value="P:cell-substrate adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11780">
        <property type="entry name" value="SH3_Sorbs_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000001">
        <property type="entry name" value="Sorbin and SH3 domain-containing protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000004">
        <property type="entry name" value="Sorbin and SH3 domain-containing protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF64">
        <property type="entry name" value="SORBIN AND SH3 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="205"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="279"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="458"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="343"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="135"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="356"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="519" mass="59027" checksum="E1DA7D1849FE7A7C" modified="2018-12-05" version="1">TVPRSKSADPVDSRGRPMPVPTRSSSLKPSEWEPPEKKVDTRKYRAEPRSIFDYEPGKSSVLKLERTSAPSFINASSFPQLSSFKISPHTCRFNVIMRGMNGSFLLKRQKASRTECPLVAFCVVFPSDSSPAHGESQDEVLRKRHGDKEKILEEQRRLKREQEEADIAARRHTGIVPTHHQFITNERFGDLLNITDNAEKRKSGVERTPAMARFDFRAESLKELPFQKGDIVYIIRQVDQNWYEGEHHGRVGIFPRSYVELLPPTEKAQPKKSAPVQVLEYGEALARFNFKGDTVVEMSFRKGERITLIRRVDENWYEGKISGTNRQGIFPVTYVEVHKRPRVKNGVDYPDPPVSLSPQRSTNASPQVSLNEADHLGRISRSPVMPYDIQENNNVNSFAEAVCNEILNIAETSVRYCSTLSHHHNDSLHRLHPHPSKQCLIISQQPQSHSSSPEPSRLSCGIFQALYSYVPQNDDELELQEGDLVSVMEKCDDGWFVGTSKRTKQFGTFPGNYVKEVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LK11</accession>
    <name>A0A3B5LK11_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Delta-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Putative Notch ligand involved in the mediation of Notch signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LK11"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009941"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010059.1">
        <property type="protein sequence ID" value="ENSXCOP00000009941.1"/>
        <property type="gene ID" value="ENSXCOG00000007538.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167177"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060429">
        <property type="term" value="P:epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046331">
        <property type="term" value="P:lateral inhibition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007498">
        <property type="term" value="P:mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050793">
        <property type="term" value="P:regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035282">
        <property type="term" value="P:segmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048863">
        <property type="term" value="P:stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000018">
        <property type="entry name" value="Delta-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000012">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000064">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.140:FF:000001">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3510:FF:000003">
        <property type="entry name" value="Delta-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000434">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001774">
        <property type="entry name" value="DSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051022">
        <property type="entry name" value="Notch_Cell-Fate_Det"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011651">
        <property type="entry name" value="Notch_ligand_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049">
        <property type="entry name" value="CRUMBS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01414">
        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21700">
        <property type="entry name" value="EGF_DL_JAG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07657">
        <property type="entry name" value="MNNL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DSL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="8"/>
    </dbReference>
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        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="match status" value="6"/>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 17" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="13 15 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 15 17" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 17 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 17 19" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017245989" description="Delta-like protein" evidence="19">
        <location>
            <begin position="22"/>
            <end position="715"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="528"/>
            <end position="551"/>
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            <begin position="166"/>
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    <feature type="domain" description="EGF-like" evidence="20">
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            <begin position="277"/>
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    <feature type="domain" description="EGF-like" evidence="20">
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    <feature type="domain" description="EGF-like" evidence="20">
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            <begin position="355"/>
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    <feature type="domain" description="EGF-like" evidence="20">
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            <begin position="395"/>
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    <feature type="domain" description="EGF-like" evidence="20">
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    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="471"/>
            <end position="511"/>
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        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
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    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="181"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
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            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
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    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="364"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="383"/>
            <end position="392"/>
        </location>
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    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="421"/>
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    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="459"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="501"/>
            <end position="510"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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            <dbReference type="ARBA" id="ARBA00022843"/>
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            <dbReference type="RuleBase" id="RU280815"/>
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            <dbReference type="PROSITE" id="PS51051"/>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009941.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    </evidence>
    <sequence length="715" mass="79228" checksum="A2FC0472B4B99E8B" modified="2018-12-05" version="1">MAAWFTFTIAFSTTLILQVFGSGVFEVDLHEFKNHKGLLANGNACTPSCRTYFKICLKNYQAVVSPGDCIFGSRETPVLGTNSFSAKDSGTLPRPIQIPFKFGWPGSFSLIIEAWHSPYGNLPVDTNNPDFMISFFAIQRQLGVGTDWSQDTQTEKQTELRYSYRFICNESYYGESCSKKCTPRDDRFGHYTCTRDGQLSCLPGWKGKYCEEPICLEGCSERNGNCSKPGECVCREGWQGTFCDECRKYPACKHGTCQLPWQCNCQEGWGGLLCDQDLNFCTHHHPCLNGATCMNTGQGSYTCTCLPGFTGVNCELEMQVCDSNPCRNGGICTNLETGYRCTCLQGFEGSHCEHNLLTCADSPCFHGGKCWEKDNGRSYMCECPSSYTGLNCEKRVDKCTSLPCANGGLCLLQGGMRMCSCRAGFTGQRCEININECAGNPCLNGGTCQDRINDYACICPAGYGGRNCDRILDECFVRPCLNGGICTTGSGPGKTPAACTCPPGYTGPLCESFATFTSPVTGDDGFQWVAVSLAVGLVALLVLLCMVGFALRHIHRQAQGQGAETETMNNLSKVQSDNLIPASQLKNTNQKLSLEVDCDSEKSNFIHKNYHLDSYNSKTKDFKDEKSQEDKSLIYDKSLEDKMPLSRMYSEKPECRISTICSSRDSMYQSVFVIAEERRECVIATEVSIFLDFKRITLSQVHYPSIMIRLFMEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLU4</accession>
    <name>A0A3B5LLU4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA-binding protein RFX2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Regulatory factor X 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLU4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011685"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011818.1">
        <property type="protein sequence ID" value="ENSXCOP00000011685.1"/>
        <property type="gene ID" value="ENSXCOG00000008821.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244879"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007368">
        <property type="term" value="P:determination of left/right symmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070121">
        <property type="term" value="P:Kupffer's vesicle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000017">
        <property type="entry name" value="transcription factor RFX3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003150">
        <property type="entry name" value="DNA-bd_RFX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039779">
        <property type="entry name" value="RFX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057321">
        <property type="entry name" value="RFX1-4/6/8-like_BCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007668">
        <property type="entry name" value="RFX1_trans_act"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619:SF17">
        <property type="entry name" value="DNA-BINDING PROTEIN RFX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619">
        <property type="entry name" value="RFX TRANSCRIPTION FACTOR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25340">
        <property type="entry name" value="BCD_RFX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04589">
        <property type="entry name" value="RFX1_trans_act"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02257">
        <property type="entry name" value="RFX_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51526">
        <property type="entry name" value="RFX_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RFX-type winged-helix" evidence="13">
        <location>
            <begin position="180"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="PI-PLC Y-box" evidence="12">
        <location>
            <begin position="396"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="650"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="297"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="308"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="651"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="694" mass="76974" checksum="21C4D5379A2AA755" modified="2018-12-05" version="1">MQNSEAASDPPTGVATLRTSSSAQAPVVQPVPASQQRVLVQATGSAQKGGQVSVSRVPQQVHSTVRTAYSYNPDSQLYGQGSGGAYFDSQAGGTHVTTVVSSASSGVPPHGMVGIAMDVGSSHIISSGSTYLLHSGGMEGNRSHISHSSRSSSAMLEMAIENLQKSEGIASHKSSLLNSHLQWLLDNYETAEGVSLPRCSLYNHYLRHCQEQKLDPVNAASFGKLIRSVFMGLRTRRLGTRGNSKYHYYGIRVKPDSPLNRLQEDTQYMAMRQQPVHQKQRFKPLQKVDSMSDSLCGSSQHCSSTPEQSVAAQTQQHQQYIDTSHSLPPFPSPDLGTQPLPERISMTDIKKLQTLYRSHCEATLDVVMNLQFHYVENFWQNFWNATAPSSDGSTTVPSSDDELERVIPREKLVSLCKYEPVRLWMRSCDHILYQALVEILIPDVLRPVPSTLTQAIRNFAKSLEGWLTTAMTSFPQEIVRTKVAVVSAFAQTLRRYTSLNHLAQAARAVLQNTSQINQMLSDLNRVDFANVQEQASWVCQCDEGVVQRLEQDFKVTLQQQSSLDQWATWLDNVVSQVLKPHQGSPSFPKAARQFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMFYLVEHRVAQATGETPIAVMESSSSPSDASFSDEMSNLDGDGAPAEPAVKRERIEIGHPLQEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLY0</accession>
    <name>A0A3B5LLY0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FACT complex subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the FACT complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peptidase M24 family. SPT16 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLY0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008869"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008975.1">
        <property type="protein sequence ID" value="ENSXCOP00000008869.1"/>
        <property type="gene ID" value="ENSXCOG00000006758.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014495"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032786">
        <property type="term" value="P:positive regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01091">
        <property type="entry name" value="CDC68-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000003">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.210:FF:000001">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000017">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000005">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000005">
        <property type="entry name" value="SPT16 homolog, facilitates chromatin-remodeling subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.210">
        <property type="entry name" value="FACT complex subunit Spt16p/Cdc68p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029148">
        <property type="entry name" value="FACT-SPT16_Nlobe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056595">
        <property type="entry name" value="Fact-SPT16_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048969">
        <property type="entry name" value="FACT_SPT16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013953">
        <property type="entry name" value="FACT_SPT16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040258">
        <property type="entry name" value="Spt16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033825">
        <property type="entry name" value="Spt16_M24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980">
        <property type="entry name" value="CDC68 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980:SF15">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14826">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24824">
        <property type="entry name" value="PH_SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08644">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21091">
        <property type="entry name" value="SPT16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01285">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01286">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FACT complex subunit SPT16 N-terminal lobe" evidence="12">
        <location>
            <begin position="5"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="FACT complex subunit SPT16 middle" evidence="13">
        <location>
            <begin position="480"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="14">
        <location>
            <begin position="757"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="871"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="878"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="928"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="957"/>
            <end position="974"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="974" mass="111496" checksum="0913D374A6EE692D" modified="2018-12-05" version="1">MAVNLDKEAYYRRIKRLYSNWKKAEDEFGKVDAIVVSVGVDEEIVYAKSTAIQTWLFGYELTDTIMVFCETKIIFLASKKKVDFLKQVAITKGNENANGVPPITLLTREKNESNKGNFDKMIEAIKGSKEGKTIGVFSKDKFPGEYMKSWSNTLSAEGLDRVDISAVVAYTMAVKEDGELNLMKKAAAITGEVYSKFFKERVMEIVDADEKVRHSKLAESVEKAIEEKKYLGGADPSTVEMCYPPIIQSGGNYSLKFSVVSDKNHMHFGAITCAMGIRYKSYCSNLVRTLMVDPSQEMQDNYNFLLQMEEELLKELKHGMKICDAYNAVMEYVKKEKPDLVAKLTKNLGFAMGIEFREGSLVLNAKNQYKLKKGMVLSISLGLSDLINKDAKKEEQKYALFLGDTVQINEVSNDFVFLCYFANGTRAHQKELANNLNEEAKRRLTEQKGEQHIQKARKSNVSYKNVSQMPKEKEIREMKIHIDKKYETVIMPVFGIATPFHIATIKNISMSVEGDYTYLRINFYVPGSSLGRQEGNIFPNPDATFVKEITYRASNLKAPGDTSVPSTNLQNAFRIIKEVQKRYKTREAEEKEKEGIVKQDSLVINLNRSNPKLKDLYIRPNIAQKRMQGSLEAHTNGFRFTSVRGDKVDILYNNIKHAIFQPCDGEMIIVLHFHLKNAIMFGKRRHTDVQFYTEVGEITTDLGKHQHMHDRDDLYAEQMEREMRHKLKSAFKNFIEKVETLTKEELEFEVPFRDLGFQGAPYRSTCLLQPTSSSLINVTEWPPFVVTLDEVELVHFERVQFHLKNFDVVIVYKDYSKKVTMINAVPVNSLDPIKEWLNSCDIKYTEGVQSLNWTKIMKTIVDDPEGFFEQGGWSFLDPESEGSGAEEDSESEMEDETFNPSADEEEEEEEEDSDEDYNYSASLGSEEESGKDWDELEEEARKADKESHYEDEDTSNRKRKARPAAPPSKKKRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMA3</accession>
    <name>A0A3B5LMA3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Protein kinase D4</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMA3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011980.1">
        <property type="protein sequence ID" value="ENSXCOP00000011845.1"/>
        <property type="gene ID" value="ENSXCOG00000008959.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183024"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20796">
        <property type="entry name" value="C1_PKD_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01239">
        <property type="entry name" value="PH_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000562">
        <property type="entry name" value="Protein kinase D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000171">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000007">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968:SF25">
        <property type="entry name" value="PROTEIN KINASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968">
        <property type="entry name" value="PROTEIN KINASE C, MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="8">
        <location>
            <begin position="94"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="215"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="291"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="619" mass="70742" checksum="2FDCBA089BECEFD3" modified="2018-12-05" version="1">MLWGLVRQGLRCEGCGLDFHKRCAFLLPNDCSRIRRQVSTSFSLFPPRRPRTHSLSSQAGGSLEEISLSKPLSRPPSWNEPPVWLGDKSRPQVPHTFHIHSYTKPTVCQYCHRLLKGLFRQGLQCSDCRFNCHRRCEPLVPRDCPGERRAFGEMFALSCFFVLTFFPPFFIQLSRLYFLPLSLACFPSILPCFSSNIPLMRLVQSAHHTKRRAAGVLREGWLLHHTNTDTLRKRHYWILDWKTITLYQNESSTKYYKEIPLSEVLQVRGPAHLAMPSLPGTDLHSFELVTASLVFCVQAGEDGAAWESAICQALMPVQSSRGPGEEHQGESTTDISSVYQIFTDEVLGSGQFGVFHSPNDNLSHHGVVVLEGMFETSRHVFVVMEKLHGDMLEMILSSEKGRLPERITRFLVIQILEALRYLHLKHVAHCDLKPENVLLVSADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVISSSGYNRSLDMWSVGVIMYVSLSGTFPFNEDEDIRQQITNAAFMYPRQPWANISLEAVSLINNLLQVAVRRRYTVGKALGHSWLQNFQLWCDLREFELRMGCRYLTHEGDEEHWSRFALERGLMFPSHLCWDSSSEMDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LN05</accession>
    <name>A0A3B5LN05_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LN05"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012367"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012516.1">
        <property type="protein sequence ID" value="ENSXCOP00000012367.1"/>
        <property type="gene ID" value="ENSXCOG00000009342.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155624"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005319">
        <property type="term" value="F:lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03263">
        <property type="entry name" value="ABC_subfamily_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000264">
        <property type="entry name" value="ATP-binding cassette, sub-family A (ABC1), member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026082">
        <property type="entry name" value="ABCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229:SF36">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY A MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19229">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER SUBFAMILY A ABCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="254" mass="28289" checksum="4072FEF4A7E67C38" modified="2018-12-05" version="1">IQNLVKVFSNCSSPAVDGLSINFYESQITAFLGHNGAGKTTTMSVLTGMFPPTSGTATIYGKDIRSDMDSIRMSLGMCPQYNILFQHMTVAEHILFYTLLKGRPIAEAEEEVENMLQDLGLPHKRDELTQNLSGGMQRKLSVALAFVGGAKVVILDEPTSGVDPYSRRSIWDLLLKYRLGRTVIMSTHHMDEADLLSDRVAIISQGRLCCCGSPIFLKNCFGAGFYLTLVRRMKHEPPNVRNWENETFCLSETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNH2</accession>
    <name>A0A3B5LNH2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein eS28</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S28</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS28 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNH2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009399"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009510.1">
        <property type="protein sequence ID" value="ENSXCOP00000009399.1"/>
        <property type="gene ID" value="ENSXCOG00000007160.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00910000144227"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000289">
        <property type="entry name" value="Ribosomal_eS28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028626">
        <property type="entry name" value="Ribosomal_eS28_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01200">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00961">
        <property type="entry name" value="RIBOSOMAL_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="53" mass="6179" checksum="E750A1B3896B83E1" modified="2018-12-05" version="1">MDASRVQPIKLARVRVEFMDDSNRSIIRNVKGPVREGDVLTLLESEREARRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LR36</accession>
    <name>A0A3B5LR36_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dickkopf family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LR36"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013512"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013678.1">
        <property type="protein sequence ID" value="ENSXCOP00000013512.1"/>
        <property type="gene ID" value="ENSXCOG00000010236.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164073"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039706">
        <property type="term" value="F:co-receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048019">
        <property type="term" value="F:receptor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23015">
        <property type="entry name" value="Dkk2_Cys1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23273">
        <property type="entry name" value="Dkk2_Cys2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.80.10:FF:000001">
        <property type="entry name" value="Dickkopf WNT-signaling pathway inhibitor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.80.10">
        <property type="entry name" value="Lipase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006796">
        <property type="entry name" value="Dickkopf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048500">
        <property type="entry name" value="DIKK1/2/4_C-subdom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048499">
        <property type="entry name" value="DIKK1/2/4_C-subdom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039863">
        <property type="entry name" value="DKK1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047303">
        <property type="entry name" value="Dkk2_Cys1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047302">
        <property type="entry name" value="Dkk2_Cys2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12113:SF12">
        <property type="entry name" value="DICKKOPF-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12113">
        <property type="entry name" value="DICKKOPF3-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04706">
        <property type="entry name" value="Dickkopf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21481">
        <property type="entry name" value="DIKK1-2-4_C-subdom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21479">
        <property type="entry name" value="DIKK1-2-4_C-subdom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017252521" evidence="8">
        <location>
            <begin position="24"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Dickkopf N-terminal cysteine-rich" evidence="9">
        <location>
            <begin position="71"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Dickkopf-related protein 1/2/4 C-terminal subdomain 1" evidence="11">
        <location>
            <begin position="165"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Dickkopf-related protein 1/2/4 C-terminal subdomain 2" evidence="10">
        <location>
            <begin position="197"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="245" mass="26891" checksum="E444498A6C52E578" modified="2018-12-05" version="1">MLDSAWSRCCFVLFLVAAVRTEASQVSEARPKANSIKPSSLEETPIQAANRGRSAGATAAAFGGSGGQVYPCSNDKECSVGSYCHSPQQASSRCLSCRRRKKRCHRDAMCCPGNRCSNYICISISESVLSPHISAIDNPNVLSTKDHSWRKKGRGQPKHVLKGHEGDPCLRSSDCSDGYCCARHFWTKICKPVLRQGEVCTKQRKKGSHGLEIFQRCDCAKGLSCKVWKDATSSSKSRLHMCQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRF0</accession>
    <name>A0A3B5LRF0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Myeloid differentiation primary response protein MyD88</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6 7">Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRF0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013380"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013545.1">
        <property type="protein sequence ID" value="ENSXCOP00000013380.1"/>
        <property type="gene ID" value="ENSXCOG00000010136.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048324"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070976">
        <property type="term" value="F:TIR domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035325">
        <property type="term" value="F:Toll-like receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002755">
        <property type="term" value="P:MyD88-dependent toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008063">
        <property type="term" value="P:Toll signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034142">
        <property type="term" value="P:toll-like receptor 4 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08312">
        <property type="entry name" value="Death_MyD88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000029">
        <property type="entry name" value="Myeloid differentiation primary response protein MyD88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000005">
        <property type="entry name" value="Myeloid differentiation primary response protein MyD88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034249">
        <property type="entry name" value="MyD88_Death"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017281">
        <property type="entry name" value="Myelin_different_resp_MyD88"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15079">
        <property type="entry name" value="MYD88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15079:SF3">
        <property type="entry name" value="MYELOID DIFFERENTIATION PRIMARY RESPONSE PROTEIN MYD88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037756">
        <property type="entry name" value="MyD88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 7" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Death" evidence="8">
        <location>
            <begin position="54"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="9">
        <location>
            <begin position="169"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="306" mass="34697" checksum="3743E8548E690F19" modified="2018-12-05" version="1">MKIIQDVGGGKNKKTIFNMDGTDSKVNLGSIPLVALNVTVRKKLGLYLNPKHTVAADWTAVAETMGFSYLEIKNYEASRNPTKTILDDWAARNSETSVGKLVSILEEVERKDIVEDLRALIDEDVRKYLETQKKKAEPPVQVPEVDSCVPRTPERSGITVDDIPGGIPEMFDAFICYCQSDFEFVLEMIRELEQTEYNLKLCVFDRNVLPGSCVWTITSELIERRCKRMVVVISDEYLDSDACDFQTKFALSLCPGARSKRLIPVVYKSMTKPFPSILRFLTVCDYTRPCTQAWFWVRLAKALSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LS33</accession>
    <name>A0A3B5LS33_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Spermatogenesis associated 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPATA2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LS33"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010614"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010738.1">
        <property type="protein sequence ID" value="ENSXCOP00000010614.1"/>
        <property type="gene ID" value="ENSXCOG00000008026.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063956"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070536">
        <property type="term" value="P:protein K63-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990108">
        <property type="term" value="P:protein linear deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060544">
        <property type="term" value="P:regulation of necroptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010803">
        <property type="term" value="P:regulation of tumor necrosis factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048839">
        <property type="entry name" value="SPATA2_PUB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15326:SF8">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15326">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 2/TAMOZHENNIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21388">
        <property type="entry name" value="SPATA2_PUB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spermatogenesis-associated protein 2 PUB-like" evidence="3">
        <location>
            <begin position="9"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="300"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="523" mass="58484" checksum="E2C9F910E61921C4" modified="2018-12-05" version="1">MDAKLREDLFRRYVTALERRPEGGYTGTGSTLEGDRSRHKDSEALLSTSTALLGAYQPDPGQRFRMVRFYEMVENSLRCQRGGNLKSLERAFHTLETICTNLLLFPWKKEFRCIKTFTGPYVYHLQSAISDAELRTLMRTIGYTCEHDSQFRLLDHPGDSSHLRQLAFELFLAQAECRLLGEVVALALRRRDSLGADIARLSVRPLDIERPYAHHLRRGSRPSKSVDVTDGAGHWHPAVNKPILKASLSLRKEPLFVDTEEDMKDEIISPVADFFPMQSPPPVDPYTSYHMSSLDDIDLYTERGGVGTGGRQTPSRPPSREPRDARDGWLLKAHGSVKCQGCGLGCSTMASCQRCDMILCSACHDIDPSPCCGLQDYHSKSPRPLDGYIPVKEKLSVYSNAQPHPHLHPHPLTLTHSHSHPHPHPQMLEKPLMPTKLFPSKSVAMTTPKAGSTERISIGGSRCGFCNKPGASHTCVNCSKVSCDSCMGLYAKDVCTRKNPQHNFVPNHQLNFKSGTISHLVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LS91</accession>
    <name>A0A3B5LS91_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 18">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="18">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 18">Golgi apparatus membrane</location>
            <topology evidence="2 18">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="18"/>
    <dbReference type="RefSeq" id="XP_027871831.1">
        <property type="nucleotide sequence ID" value="XM_028016030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LS91"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014893"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015079.1">
        <property type="protein sequence ID" value="ENSXCOP00000014893.1"/>
        <property type="gene ID" value="ENSXCOG00000011287.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114143732"/>
    <dbReference type="KEGG" id="xco:114143732"/>
    <dbReference type="CTD" id="100310831"/>
    <dbReference type="GeneTree" id="ENSGT00940000155456"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000017">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000012">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF37">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 18" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13 18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 18" id="KW-0430">Lectin</keyword>
    <keyword evidence="17 18" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="19">
        <location>
            <begin position="159"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B lectin" evidence="20">
        <location>
            <begin position="472"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="609" mass="69740" checksum="446CC6BF0C13DBF1" modified="2018-12-05" version="1">MACTRRTKTLVSTCVILSGMTNIVCLLYVGWVTNYIANIYIKVPMPLAVGKLEGDKKGDTLRIMERLDRLESVVNQHIQETPGKGEDGLPDTSFSDSSLFAHWGQELSPDNRRVALKMFQYYGYNAYLSDRLSLSRPIPDLRPDGCRNITYPLNLPQVSIVFIFVNEALSVILRSIHSAINRTPSHLLKEIILVDDNSNNDELKEKLQDFVSETNSQHPGFIKIVRHTRQEGLIRSRVSGWRAATAPVVALFDAHVEFNVGWAEPILLRIKEDRTRIISPSFDNIKYDTFEIEEYPLSAQGFDWELWCRYLNPPKTWWHRGNKSAPIQSPALIGCFVVDRLYFEEIGLLDEGMEVYGGENVELGIRVWQCGGSVEVLPCARIAHIERAHKPYTEDLTSHVRRNALRVAEVWMDEFKSHVYMAWNVPIEDSGIDIGNISERKALRKRLQCKTFRWYLVNIYPEMRMYSDTIAYGVLRNSLKNDLCLDQGPDNDNVPILYLCHGMTPQNVYYTSNQQFLVGLLSPTVDDDDNKCLVDVNGRPRLIECNYAAAKRMKLHWLFTQGGSIQNRKSKRCLELVVNSDNEFGYQLALQKCAGQKWFITNVLFSNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSJ9</accession>
    <name>A0A3B5LSJ9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSJ9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010909.1">
        <property type="protein sequence ID" value="ENSXCOP00000010784.1"/>
        <property type="gene ID" value="ENSXCOG00000008140.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000178222"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="124" mass="14610" checksum="FD27C57CADEBDB37" modified="2018-12-05" version="1">MLRGWWARYSTMSGVIPYPSLSMRALHFSSIFMMSRLPDSAQSWRAVFPFTLFRFTSAPRSRRNLRGVVYRGSVFQQRLHHGEVTRCRSRPQRWSPLDSLAVKVDRAPEFNISFTMFEQVVYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LT14</accession>
    <name>A0A3B5LT14_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19 20">CDP-diacylglycerol--inositol 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="5 20">2.7.8.11</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the biosynthesis of phosphatidylinositol (PtdIns) as well as PtdIns:inositol exchange reaction. May thus act to reduce an excessive cellular PtdIns content. The exchange activity is due to the reverse reaction of PtdIns synthase and is dependent on CMP, which is tightly bound to the enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a CDP-1,2-diacyl-sn-glycerol + myo-inositol = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11580"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:11581"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:11582"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.11" evidence="5 20"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LT14"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015148"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015338.1">
        <property type="protein sequence ID" value="ENSXCOP00000015148.1"/>
        <property type="gene ID" value="ENSXCOG00000011478.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154169"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003881">
        <property type="term" value="F:CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000003">
        <property type="entry name" value="CDP-diacylglycerol--inositol 3-phosphatidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014387">
        <property type="entry name" value="CDP_diag_ino_3_P_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362:SF4">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--INOSITOL 3-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000848">
        <property type="entry name" value="CDP_diag_ino_3_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 20" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 20" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="16 20" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00057387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00070582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRNR" id="PIRNR000848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="197" mass="21726" checksum="BE2E0047951F715A" modified="2018-12-05" version="1">MTQENIFLFVPNLIGYARVVLALLSFYLMPSSPWPAVFCYLLSALLDAFDGHAARALDQGTRPTKFGAMMDMLTDRCATMCLLLSMSLDITSHWLHLHSSTVKGSGSHKTIDLSGNPILRLYYTSKPVLFVMCAGNELFFCLLYILHHIQNPAGWLRPLLVLCGAISLLKAAISVLHLVTASQNMAALDAAERAKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LT87</accession>
    <name>A0A3B5LT87_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor 38B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">May be required for pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PRP38 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027877010.1">
        <property type="nucleotide sequence ID" value="XM_028021209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LT87"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011024"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011151.1">
        <property type="protein sequence ID" value="ENSXCOP00000011024.1"/>
        <property type="gene ID" value="ENSXCOG00000008329.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114146815"/>
    <dbReference type="KEGG" id="xco:114146815"/>
    <dbReference type="CTD" id="55119"/>
    <dbReference type="GeneTree" id="ENSGT00730000111085"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005037">
        <property type="entry name" value="PRP38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23142">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 38A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03371">
        <property type="entry name" value="PRP38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="212"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="237"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="267"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="325"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="389"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="404"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="430"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="463" mass="54844" checksum="74869D50FD61AFFA" modified="2018-12-05" version="1">MANVGNQLPTQAVSKPPSGKHGNVLPLWGNEKTMNLNPMILTNVLSSPYFKVQLYELKTYHEVVDEIYFKVTHIEPWEKGSRKTAGQTGMCGGVRGVGTGGIVSTAFCLLYKLFTLKLTRKQVMGLITHTDSPYIRALGFMYIRYTQPPTDLLDWYNDFLDDEEELDVKAGGGCVMTIGEMLRSFLTKLEWFSTLFPRIPVPVQKAIDQQMKARPRKVAQKEPEEEAEFTESGRQGERRHSRTPRRTPSPKRSPKRSRSRSHHRERDRHGPSFDRELERERDRQRKERDGGRDRERDRADRGDREKRRSRSADRNREHKRSLSGSRERRGERREKERDGAEDRSRKKDRDHHKDRGTERDRSRDRKSRTENDERRHKEDRDRHREDRKAKRSSRSRSREKRHKGGAEEKSRKRDRSHSRERERERDGERSHKRSRSKERSRHQRESSNEHSKHGERRRSQSTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTG4</accession>
    <name>A0A3B5LTG4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTG4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014397"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014581.1">
        <property type="protein sequence ID" value="ENSXCOP00000014397.1"/>
        <property type="gene ID" value="ENSXCOG00000010925.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161707"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004938">
        <property type="term" value="F:alpha2-adrenergic receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051379">
        <property type="term" value="F:epinephrine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071881">
        <property type="term" value="P:adenylate cyclase-inhibiting adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030168">
        <property type="term" value="P:platelet activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006940">
        <property type="term" value="P:regulation of smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019229">
        <property type="term" value="P:regulation of vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000716">
        <property type="entry name" value="Adrenoceptor alpha 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000100">
        <property type="entry name" value="alpha-2B adrenergic receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002233">
        <property type="entry name" value="ADR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000735">
        <property type="entry name" value="ADRA2C_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF25">
        <property type="entry name" value="ALPHA-2C ADRENERGIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01103">
        <property type="entry name" value="ADRENERGICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00560">
        <property type="entry name" value="ADRENRGCA2CR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="22"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="41"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="214"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="441" mass="49996" checksum="4A3C661AB2DBE2CD" modified="2018-12-05" version="1">ADAPYVGNISSNSTSEQPYTQLAIWGLAGLVSFLILFTIVGNVLVVIAVLTSRALKPPQNLFLVSLASADILVATLVMPFSLANELMGYWFFGKIWCDIYLALDVLFCTSSIVHLCAISLDRYWSVTQAVEYNLKRTPKRVKGMIIVVWLISAVISFPPLISMDRSSNERKPMCQLNDVTWYILYSSIGSFFAPCVIMILVYIRIYQVAKTRTRTMSEKKRSVDSPLENGMDQAEPGMGGSIKSTQGGSTKERERENGHCQEQTVGSPRSNEPKPPLNNHEDEFDDSSSSDEKPKKCIGSSKQSQNNRKDRKSSRKSSSASKYSSRKSRSSSKSLELFSSRRKRRSTVNRKKISAAREKRFTFVLAVVMGVFVVCWFPFFFSYSLYGICRNSCKIPDVLFKFFFWIGYCNSSLNPVIYTIFNQDFRRAFQKILCNSWKRSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUW0</accession>
    <name>A0A3B5LUW0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUW0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014650"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014836.1">
        <property type="protein sequence ID" value="ENSXCOP00000014650.1"/>
        <property type="gene ID" value="ENSXCOG00000011118.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156528"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000768">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000705">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000002">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF3">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="13">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="406"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="96"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="313"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="354"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="369"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="435"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="681" mass="76173" checksum="A43DBE4A10D886AF" modified="2018-12-05" version="1">MFRKKKKKRPEISAPKNFEHRVHTSFDAKRGCFVGLPMQWQSLIENLRRPRPMVDPSRITEVELRPKKTIVRGSMVGHGEYISAMINDMSRLSVTSSNSLRKSSPSARKRAQSLGRLGEDGYQYEGLTQDDEDDMDESQDHWRDKGRNIHSETNTPYLGMKKSITLQPNGILPKAKSTYEVGVSSVEGPLQPPPPQNIPPPSYGPYLSGELGSPQERLIWKRDFQLPRGMPPNQRPVACFYSPAVALQQLHGDQGNIGPELRPNLPMYMHAQNSPGRPFSSYDLKFLPTGTSSPLVGGIRPQRMVRSSASYTLGLSPNMGLRPGVPDSFLRHSGCPNTPYPRQDSPSQPRPSPTGSLATSPPGTCSPAFRPPQHPAPRPPPDPPKVTHEQAALQMVVDKGDPRTYLENFVKIGEGSTGVVCIATEKHTGRQVAVKMMDLRRQQRRELLFNEVVIMRDYQHKNVVEMFKSALVEEELWVIMEYLQGGALTNIVSETLSEEQIATVCEAVLQALAYLHSQGVIHRDIKSDSILLTLDGRVKLSDFGFCAQISKDIPKRKSLVGTPYWMAPEVISKSPYGTEQVDVWSMGIMVVEMVDGEPPYFSETPVAAMKRLRDEPAPTVRNVSQQISPVLKDFLDRMLTRDPLERASATDLLEHPFLLQSGSPQCLVPLVEQYRKRMSRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUW2</accession>
    <name>A0A3B5LUW2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cell death-inducing p53-target protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">LITAF-like protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CDIP1/LITAF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUW2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014655"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014841.1">
        <property type="protein sequence ID" value="ENSXCOP00000014655.1"/>
        <property type="gene ID" value="ENSXCOG00000011125.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157696"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098560">
        <property type="term" value="C:cytoplasmic side of late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098574">
        <property type="term" value="C:cytoplasmic side of lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042771">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006629">
        <property type="entry name" value="LITAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037519">
        <property type="entry name" value="LITAF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292:SF7">
        <property type="entry name" value="CELL DEATH-INDUCING P53-TARGET PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23292">
        <property type="entry name" value="LIPOPOLYSACCHARIDE-INDUCED TUMOR NECROSIS FACTOR-ALPHA FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10601">
        <property type="entry name" value="zf-LITAF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00714">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51837">
        <property type="entry name" value="LITAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LITAF" evidence="13">
        <location>
            <begin position="124"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="210" mass="22400" checksum="013EBA0C9441F32D" modified="2018-12-05" version="1">MSSDPPPPYPGGPSAPLIEEKNGQPGNEWKLARVTTQPQGQPLLPDYGPPPYEGPQPGFLPPHVPGEGPMPMPMPMPPPMQGGPYPPPPGHFGQPVPGQMGPGPSHFVHVGSHTATVLAPPGAATTVTVLQGEMFQTSPVQTVCPHCQQAIITRISHDVGLMNTLFCLFCFFVGCDLGCCLIPCLIDDLKDVTHTCPNCKGYIYTYKRIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LW98</accession>
    <name>A0A3B5LW98_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EML-like second beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LW98"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013591"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013758.1">
        <property type="protein sequence ID" value="ENSXCOP00000013591.1"/>
        <property type="gene ID" value="ENSXCOG00000010288.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155564"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000042">
        <property type="entry name" value="echinoderm microtubule-associated protein-like 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050630">
        <property type="entry name" value="WD_repeat_EMAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720:SF33">
        <property type="entry name" value="HELP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720">
        <property type="entry name" value="WD-40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="EML-like second beta-propeller" evidence="3">
        <location>
            <begin position="20"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="263" mass="29093" checksum="CE051695869A9588" modified="2018-12-05" version="1">NWSGFSVSSLFWDPIGSVFIRIWGLATHPFKDKLLNKVSLGHPAKCTCYSPNAEMVSIGMENGEFIVLLVNSLTVWGKKRDRSVSIQDIRFSPDNRFLAVGSVESAVDFYDLTLGPSLNRIGYCKDIPGFVIHIDFSADSKHIQVIWVQGLVPSGKIVTELNVIERITWATWTSIFGDEVLGVWPRNADKADVTCACVSHAGLSLVTGDDFGLVKLFDFPCPEKFHKRYFGHSAHVTNVRFSCDDKYVISAGGSDCRSEPGPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWS7</accession>
    <name>A0A3B5LWS7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWS7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012289"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012435.1">
        <property type="protein sequence ID" value="ENSXCOP00000012289.1"/>
        <property type="gene ID" value="ENSXCOG00000009290.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000203589"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019372">
        <property type="entry name" value="LHFPL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12489:SF19">
        <property type="entry name" value="LHFPL TETRASPAN SUBFAMILY MEMBER 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12489">
        <property type="entry name" value="LIPOMA HMGIC FUSION PARTNER-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10242">
        <property type="entry name" value="L_HMGIC_fpl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="193" mass="21406" checksum="75320096412A50A1" modified="2018-12-05" version="1">MCHVIVTCRSMLWTLLSIVVAFAELVAFMSPEWLLRPPLPENGVTQTKVNDRESLGLYNRCYRIKGEISCGIYARAFGEVASKFWQAAMLFLAAGMLVLGCVACISVFTLCFQSIKKKSIFNICGLLQAIGGKDVLVLLNGGNLEKCSSCLSACSLAVLSKRWSWTATLQVSILWHTLILLFIYLQPLLTFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWV0</accession>
    <name>A0A3B5LWV0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Hydroxysteroid dehydrogenase like 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWV0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013806"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013975.1">
        <property type="protein sequence ID" value="ENSXCOP00000013806.1"/>
        <property type="gene ID" value="ENSXCOG00000010449.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160053"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd05356">
        <property type="entry name" value="17beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052149">
        <property type="entry name" value="17-beta-HSD3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44889">
        <property type="entry name" value="INACTIVE HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44889:SF1">
        <property type="entry name" value="INACTIVE HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="317" mass="34395" checksum="175350E7037D378F" modified="2018-12-05" version="1">MAAAVDSFHLLYREVSRSCSSHFEALALIGALYAASRAVVLLRDCCTVARVHFLPRMMPSGRLTQRYGDWAVIYGAAEPVAGAYAEELARSGISIIFITQDGSSVRNAASFFSQTYGVETAVVTADFSLGQAAAKPVTEALRGKDIGFLVNCMDETSASPRSLIEIPEQGVLDRVNRNVGAATLMARLVLPGMVERSRGAVVNISSGACCRPRHGRVTLSAFTGYLDNFSRALHFEYSDKGIFIQSLIPFQISSSSSAEGWFVPSPEVYARHAVSTLGVSNRTTGYWPHTLQYGLMKCIPEWIWILGSDASCVSCFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWW1</accession>
    <name>A0A3B5LWW1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the histone H2B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWW1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015627"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015821.1">
        <property type="protein sequence ID" value="ENSXCOP00000015627.1"/>
        <property type="gene ID" value="ENSXCOG00000011668.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000267181"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000003">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 12" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="14">
        <location>
            <begin position="50"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="28"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="151" mass="16660" checksum="C172757AFDB07301" modified="2018-12-05" version="1">WNWQGNLCTSTSNIPVYVVKVNKMGGKMPEPAKSAPKKGSKKAVTKTAGKGGKKKRRTRKESYAIYVYKVLKQVHPDTGISSKAMSIMNSFVNDIFERIASEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LX93</accession>
    <name>A0A3B5LX93_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000013956"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014128.1">
        <property type="protein sequence ID" value="ENSXCOP00000013956.1"/>
        <property type="gene ID" value="ENSXCOG00000010566.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154551"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187">
        <property type="entry name" value="ALPHA KINASE/ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187:SF5">
        <property type="entry name" value="HEAT SHOCK 70 KDA PROTEIN 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="414" mass="47359" checksum="77D3465BCE4C8866" modified="2018-12-05" version="1">MSNTILISISYAFNVKPREEGGETQIKRWSNGLGLDTPKTPTCILFDEHEEFMKFGYEAKTEFNEMREGEAEKYYFFDNVNIAIQGKVSISSNHTDLHTIKAANDKSMKVLKVFTEVLRFLKDDALKTIRSHPEGGEFTVFDFTWVLTVPELLKDFCKKFIIEAATQAGLVTDDTKDKVMFVLESEAALTWCLKLQSDGFITQNHKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEDVQISCPDNLRDEAQKNKEIETFFDSVEGASWDDGSIRISWEKVKSFYDESLQGITKKLRKILNKNLNIGFIVLVGGFAESQILYQHITDQFEPQYKVLCPLRPQEAILKGAVELGRNPKLVESQRKHPTLHFTLLEISYISIYFTMFSKIYSQVGTFCDILKISAFIMHILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZK6</accession>
    <name>A0A3B5LZK6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZK6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016405"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016612.1">
        <property type="protein sequence ID" value="ENSXCOP00000016405.1"/>
        <property type="gene ID" value="ENSXCOG00000012386.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000179440"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050874">
        <property type="entry name" value="Diverse_PLD-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10185:SF9">
        <property type="entry name" value="INACTIVE PHOSPHOLIPASE D5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10185">
        <property type="entry name" value="PHOSPHOLIPASE D - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="70" mass="7880" checksum="ED5334A04FC7936B" modified="2018-12-05" version="1">MVTDNAVYLGTHDWVGSDFAINAGVGLVVRMKDSTKDTGATILEHIKASFERDWRSRYLYVQQTGTHCVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0S3</accession>
    <name>A0A3B5M0S3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027865243.1">
        <property type="nucleotide sequence ID" value="XM_028009442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0S3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017698"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017922.1">
        <property type="protein sequence ID" value="ENSXCOP00000017698.1"/>
        <property type="gene ID" value="ENSXCOG00000013344.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114139472"/>
    <dbReference type="KEGG" id="xco:114139472"/>
    <dbReference type="CTD" id="9363"/>
    <dbReference type="GeneTree" id="ENSGT00940000160863"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="CDD" id="cd04115">
        <property type="entry name" value="Rab33B_Rab33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000516">
        <property type="entry name" value="RAB33B, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR041822">
        <property type="entry name" value="Rab33A/B"/>
    </dbReference>
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        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
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        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
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        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="282" mass="30750" checksum="D83C97E00D259992" modified="2018-12-05" version="1">MTKDSPEEETRAPGGVGVGGGEGGGGGGGGAGRGASRRNRADDNVTILTSSMDFYPAGRSRASSSSDAAPSLTSSLDTSIQTRIFKIIVIGDSNVGKTCLTFRFTGGSFPDKTEATIGVDFREKAVEIEGETIKVQVWDTAGQERFRKSMVEHYYRNVHAVVFVYDVTKMASFRNLQTWIEECNGHRVSASVPRVLVGNKCDLVDQIQVPSNMALKFADSHNMLLFETSAKDPKESQNVDSIFMSLACRLKAQKSLLYRDVEREDGRVRLTQETEAKTNCPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M198</accession>
    <name>A0A3B5M198_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Coronin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">F-actin regulator involved in anterograde Golgi to endosome transport: upon ubiquitination via 'Lys-33'-linked ubiquitin chains by the BCR(KLHL20) E3 ubiquitin ligase complex, interacts with EPS15 and localizes to the trans-Golgi network, where it promotes actin polymerization, thereby facilitating post-Golgi trafficking. May play a role in the maintenance of the Golgi apparatus morphology.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the WD repeat coronin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M198"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013794"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013963.1">
        <property type="protein sequence ID" value="ENSXCOP00000013794.1"/>
        <property type="gene ID" value="ENSXCOG00000010443.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159328"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015505">
        <property type="entry name" value="Coronin"/>
    </dbReference>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856:SF20">
        <property type="entry name" value="CORONIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="78"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU280818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="177" mass="19150" checksum="669ABA8E555240EE" modified="2018-12-05" version="1">FFYFLVNYTSSTIVFGKSGKIYLFGWINNVHAGSFSCQGRHITASSNLVAFNTDQAGGGMVGLTSVSPSDGQWTVTQISCHSDLVTDMDFSPFDECLLATCSGDETVKLWRLCDPEQEQPSSPELTLQSGQGRLELVHFHPTSSGLLVVATAKSPLIWDTSRQDAPLAGNRVQNALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M287</accession>
    <name>A0A3B5M287_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tyrosine-protein phosphatase non-receptor type 9</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Protein-tyrosine phosphatase that could participate in the transfer of hydrophobic ligands or in functions of the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein-tyrosine phosphatase family. Non-receptor class 3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M287"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018208"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018439.1">
        <property type="protein sequence ID" value="ENSXCOP00000018208.1"/>
        <property type="gene ID" value="ENSXCOG00000013714.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165569"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14543">
        <property type="entry name" value="PTPc-N9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000005">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000026">
        <property type="entry name" value="tyrosine-protein phosphatase non-receptor type 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF328">
        <property type="entry name" value="PROTEIN TYROSINE PHOSPHATASE NON-RECEPTOR TYPE 9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="13">
        <location>
            <begin position="74"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="11">
        <location>
            <begin position="451"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="12">
        <location>
            <begin position="635"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="297"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="305"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="360"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="387"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="399"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00055430"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00060781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50056"/>
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        <source>
            <dbReference type="PROSITE" id="PS50191"/>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="746" mass="84022" checksum="94AFCE04266D87FB" modified="2018-12-05" version="1">MAEALTTQEQLAVEEFLREVRSREHPHSAGLVSQPTAVKFLMARKFDVSRAIDLFQAYKNTRIKEGIININPDEEPLRSELLSGKFTVLPGRDANGAALALFTARLHRPDITTHKAVLQAIIYQLDKAIESLETQKDGLIFIYDMTNSSYGNFDYELCVKILNLLKGAFPARLKCVFIVSSPLWFRAPFAVLRLFVREKLRERVSTVRAHELVSHIPVSSLPEHLGGTSQYSHVAWIQSCVNMQSNTAQGDTQEHDTHDCVGSLLRSYSLDNSNASIGTVPSHTLQGTERAVANANCYDDNNANPQNHTGNHQHWNGTALGGANTGVGPNSNMYGRAPTQSDTPPNTPLSQKADDDPAAGTTTNPASRFQNEAVKEEDEDEEREGVPPLPQKALPRPPNQAASQSPALSSSWGPDDDENLYTEVSVHMPDQGGMTVHQLVEYVKRKKKKGIYQEYEDIRKEPPAGTFDYSKKISNQIKNRYSDVLCLDQSRVRLCQLCDDEDETSDYINASFMDGYKRSNAYIATQGPLPKTFVDFWRMVWEQMVLIIVMTTRVVERGRVKCGQYWPLEEGRTEQHGYFMVKNTHIQVFQDFKLSYLELYNTQSGEKREVCHYLYVSWPDFGVPKSASAMLDFREHVLERRKAAVQSLGSSWRGPQGGPPVVVHCSAGIGRTGTFCTLDICLSQLEDVGTVNVHQTVRRMRTQRAFSIQTWDQYYFCYTAVIEYAQRHGRLSPVQWSDSELETDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2T5</accession>
    <name>A0A3B5M2T5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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            <taxon>Xiphophorus</taxon>
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    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M2T5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018418"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018650.1">
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        <property type="gene ID" value="ENSXCOG00000013868.1"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
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        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
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        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX CONTAINING 15"/>
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    </dbReference>
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        <property type="entry name" value="ANKYRIN"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="14"/>
            <end position="46"/>
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            <end position="143"/>
        </location>
    </feature>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="281" mass="31491" checksum="2DAA585CAE740931" modified="2018-12-05" version="1">MASMDATLEEQTKDGDTALTLAAEANQVENIKLLLRHGASPHNTNSRNESPLVLRTLIPVTTKRAIRLSGQSPVHSAADGGQTECLELLIQNGFDINTQLDKHISENYGDLRKTPLYFAVSNGDVTGAEILLKAGARTDLDPLRCILVAVRAERYELVKLLLSYGAEVNCYFRVINNTAFPTALYYCLRDHDMLRLLLNSGYQAHKRVPSKLNLNLTLNDNIYIFKYFTSCRCFQCFHNESEDLDSTWTDLHNQAYKIYCQPNVIPVRTDTPAFLPPPLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4E3</accession>
    <name>A0A3B5M4E3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MYG1 exonuclease</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MYG1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4E3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018322"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018553.1">
        <property type="protein sequence ID" value="ENSXCOP00000018322.1"/>
        <property type="gene ID" value="ENSXCOG00000013792.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010265"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003226">
        <property type="entry name" value="MYG1_exonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11215">
        <property type="entry name" value="METAL DEPENDENT HYDROLASE - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11215:SF1">
        <property type="entry name" value="MYG1 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03690">
        <property type="entry name" value="MYG1_exonuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="45247" checksum="866C7FAB2D306CA5" modified="2018-12-05" version="1">MWSPRVIALKLTKISHSVGHKIPLCHLKGLHFLCGRFRFSSANNCDAEQRFMSSEPKRIRIDEMGIKKIGTHNGTFHCDEVLACFFLRQLPEYAEAEIVRTRDTAVLAECDVVVDVGGEFDPKRHRYDHHQRTFAENFHSLCPEKPWVTKLSSAGLVYLHFGRRLLAQLTQMKDDDRQLNMLFDKLYENFVEEVDAVDNGISQCDGEVRYAVSSTLSARVAHLNPRWNSKSQDTEDGFRKALKMVGEEFLDRLDFYKSSWLPARAVVEQAFKERHQVDPSGEVLLFSQGGCPWKEHLFALEKELDVETPIKFVLYSDQNGQWRVQCVPAGLNTFQNRLSLPEEWRGVRDEALSELSGIKGCIFVHAGGFIGGNKTQEGALEMARRTLQAAAQSPANGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4L2</accession>
    <name>A0A3B5M4L2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligodendrocyte transcription factor 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027857015.1">
        <property type="nucleotide sequence ID" value="XM_028001214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4L2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014909"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015095.1">
        <property type="protein sequence ID" value="ENSXCOP00000014909.1"/>
        <property type="gene ID" value="ENSXCOG00000011302.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114134539"/>
    <dbReference type="KEGG" id="xco:114134539"/>
    <dbReference type="CTD" id="324857"/>
    <dbReference type="GeneTree" id="ENSGT00940000166262"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045664">
        <property type="term" value="P:regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19725">
        <property type="entry name" value="bHLH_TS_OLIG2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000031">
        <property type="entry name" value="Oligodendrocyte transcription factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF161">
        <property type="entry name" value="BHLH TRANSCRIPTION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="70"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="38"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="178"/>
            <end position="202"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="256" mass="27687" checksum="CE17BB988669960B" modified="2018-12-05" version="1">MDSDASSTCSRSSSPDLVVDDSAGSFFSNTIFQKYCQENRNDSEADQGRMERGSGKTKSRSELSKEEMQDLRLKVNSRERKRMHDLNQAMDGLREVMPYAHGPSVRKLSKISTLLLARNYILMLSSSLEEMKKLVGDVYGGGAAAQSRSTAHSTINHAATSVHLPLHPLAQSLHSLVGSTPTALQHHSSPPSSAPAPHSPPSASFLGFHAPVQGLLKDPLHLGGSYRHFPGMPCPCTLCQPLPTATSTLHSLSMNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6H3</accession>
    <name>A0A3B5M6H3_9TELE</name>
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        <submittedName>
            <fullName evidence="12">Ectodysplasin A receptor</fullName>
        </submittedName>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="38"/>
            <end position="65"/>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8X1</accession>
    <name>A0A3B5M8X1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Interferon regulatory factor 4a</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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        <property type="match status" value="1"/>
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    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="IRF tryptophan pentad repeat" evidence="8">
        <location>
            <begin position="15"/>
            <end position="123"/>
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    </feature>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <sequence length="472" mass="54509" checksum="11A8FD2BAB0C30FB" modified="2018-12-05" version="1">MNLDEDGGLSGSCGNGKLRQWLIEQIDSRRYPGLVWENEEKTIFRIPWKHAGKQDYNREEDAALFKAWAMFKGKFKEGVDKPDPPTWKTRLRCALNKSNDFDEIVERSQLDISEPYKVYRIIPEGTKKGMKMNRVEETPSHPLGYVPPYTSLHNQVSRLAISFKEKVPSFVVSPERRDWRDFHAPSEQHSLPNANHHGQHTDLQYGQCHYQSPISRVWPSSHTENGFQLSFHTYFSESQPPMYTMDHNNAMSDFSLHVSLYYRETLVKEVTVTTPEGCRITSTSPSSPSSSSSSSPCSEDRFHSGAEIVLFPAPYPESQRQGAELLPNILEKGVLLWMTADGLYAKRLCQGRVYWEGPLAPYVDKPNKLEKEQPCKLFDTHQFLVDLQDFVHNGRHIPRHQVVLCFGDEYPDPQRPRKLITAQIEPMFAKKLVYYYQQNTGHYLRAYDHIQEPNTAPAIDYPPQRPVQHIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M967</accession>
    <name>A0A3B5M967_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ankyrin repeat and SOCS box containing 10</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ankyrin SOCS box (ASB) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M967"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020077"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020324.1">
        <property type="protein sequence ID" value="ENSXCOP00000020077.1"/>
        <property type="gene ID" value="ENSXCOG00000015054.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158974"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03723">
        <property type="entry name" value="SOCS_ASB4_ASB18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051573">
        <property type="entry name" value="Ankyrin-SOCS_box_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24136:SF18">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24136">
        <property type="entry name" value="SOWAH (DROSOPHILA) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="118"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="150"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="183"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="217"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="250"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="291"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="7">
        <location>
            <begin position="419"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="464" mass="51846" checksum="0CB89DC575F8631C" modified="2018-12-05" version="1">MSQDSFVFSSTALSSLELDKDMLERHNYKTQLATHHLSSYILKKEAMERAQRRSTFARRPTVCQDVVIHNALYTGDVEALKRFFPRGSMANLIIEPQGGEMRWMARGEGLWSLTYEQELTTPLHITAGRGFVDCLSLLLQRGANVDLAPGGTTALHEACESCQAECAKLLLIYGANANAVSEDGLMPLHVCTSSESFECAKYLLQYGATINGCTLDEDDAPLHIAARHGLHDHVELYLRHGAAVDKQNDEGLTPLNAACAHPQEIKDLERYLKVCQMLLDAGADVHTKDQDKHTPLHMACKNANPDVVDLLLAKGSSVNNMDYGGEAPMHNILKVVCYKVSHHPERIVRALLNHGSIRVWPGALPVVLKHCSESPHTIEVLLNAYSHLKVTDTWVESVPEDAFKHKDFYESIFSLAMTPRSLQHLARCRLRSFLEGRVHKVVPKLNLPTFIKDYLLLKCRGYVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9C7</accession>
    <name>A0A3B5M9C7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BCL2 associated athanogene 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027890287.1">
        <property type="nucleotide sequence ID" value="XM_028034486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9C7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020878"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021134.1">
        <property type="protein sequence ID" value="ENSXCOP00000020878.1"/>
        <property type="gene ID" value="ENSXCOG00000015633.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114154951"/>
    <dbReference type="KEGG" id="xco:114154951"/>
    <dbReference type="CTD" id="9530"/>
    <dbReference type="GeneTree" id="ENSGT00940000158936"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039773">
        <property type="entry name" value="BAG_chaperone_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329:SF10">
        <property type="entry name" value="BAG FAMILY MOLECULAR CHAPERONE REGULATOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12329">
        <property type="entry name" value="BCL2-ASSOCIATED ATHANOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00264">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAG" evidence="3">
        <location>
            <begin position="299"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="50"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="50"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="188"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="377" mass="41058" checksum="D1584B453E7DE6C3" modified="2018-12-05" version="1">MQSNLKPDWPSTCNSGNNHGNWNSAMDAPQYPGYPSHYWYPQSHSTGHYANTYPSGSDVQQQYNPQVMPGGYPNGHGVYNTAQNQYPASGFHPSNPFYCADPQRSSYPSQGCPAEQSSGPSGQPHTQHQHHHYSGPHCQGGPGYPSGPYSHYSEGGHAVPPNPPYPTGQALHPSPQGDAWGHTGPYAPSQQQWHPGQQPSQNHYGNHVRPAHPPAWPGTGTGAPPPYQPKDQQHQRTPQVGPKPRSAPAPNPPNGKPAEISSPPQIYSKAGRSDPSPSQPEPQPAAAPVGPQPLSDNPGLAKVQQVMARVQLLQEDVDEFVGRKTDKSYRCLEELLTKELLELDSVETQGQETVRQARKEAVQRIQAILDQLEKKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9T3</accession>
    <name>A0A3B5M9T3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9T3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016734.1">
        <property type="protein sequence ID" value="ENSXCOP00000016524.1"/>
        <property type="gene ID" value="ENSXCOG00000012475.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000174101"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042289">
        <property type="term" value="F:MHC class II protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990782">
        <property type="term" value="F:protein tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035723">
        <property type="term" value="P:interleukin-15-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042110">
        <property type="term" value="P:T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11422:SF5">
        <property type="entry name" value="DIVERSE IMMUNOGLOBULIN DOMAIN-CONTAINING PROTEIN 1.1 ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11422">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="103"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="284" mass="31766" checksum="288E0B64C943B8F0" modified="2025-10-08" version="2">MSDSCRSHVCSKQVQFFILVSSDQVLWSGLGVFKGFPNGLNFCYTLNVQIGGLVRYSEEVTGGRVNRDSDKSSRTTVTSNCSLVLSDLTVGDAGSYVCLESKEAVTDVYLSLLSVRSGSPITELRPGGNLFLTCILFTYYDAGSCKPYSNVFRVNWLNEDGEQLPRDESRTKLLTENTRCNVTLLIKLQAEDNNRKWRCQVDITENKKVTFLDFRSSFLFQNTDTDQTVKPPVTEECPAELPISRIVLCVVLPLLVCIVGFFTWKSDHKRATTSAAVIELQDVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MB40</accession>
    <name>A0A3B5MB40_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MB40"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018718.1">
        <property type="protein sequence ID" value="ENSXCOP00000018486.1"/>
        <property type="gene ID" value="ENSXCOG00000013912.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="86" mass="9843" checksum="8121175BACD8CA25" modified="2018-12-05" version="1">MLAISSTPFQERGQGPSSLSTSLQGTYMLQRDWTERRRLFTRFELSLSLSSRSRTSMTVSPNSWKAPTSAVWQNCHLQVTWGSLWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDE8</accession>
    <name>A0A3B5MDE8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Calmodulin-lysine N-methyltransferase</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDE8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017729"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017953.1">
        <property type="protein sequence ID" value="ENSXCOP00000017729.1"/>
        <property type="gene ID" value="ENSXCOG00000013362.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002168"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018025">
        <property type="term" value="F:calmodulin-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025800">
        <property type="entry name" value="CaM-Lys-N-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13539">
        <property type="entry name" value="CALMODULIN-LYSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13539:SF3">
        <property type="entry name" value="CALMODULIN-LYSINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="302" mass="34016" checksum="A47313D40509836A" modified="2018-12-05" version="1">MEKGSTGSTEKTQSAFPCKDKSDEDSRGPNSHNSREATCGATAGRARWTLLRQVLCQKQTDSPEVKQVSVRRFASFDLFRRKKRSAQEHCDTSDDQWVEYRSVYYPEYVTNTFLVCWVLFFIFLHGYIGGLFFCSVLFNFSVSFLSLPGSQVAISADVKEVLLSDGNEKSIQNVQRVVESNRRAGKFGSTHVSSRVLRWDCEVDISALEGHFDTVMCADWYVYTHTHTHPHTQIYLRMCPLLTLYHFLHDGPCVSPPSPPPVPGPSPDSATHTHIHTHAPMPARIHFTPNCLVFMLSVPANG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDS3</accession>
    <name>A0A3B5MDS3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Helicase, POLQ like</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDS3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021680"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021944.1">
        <property type="protein sequence ID" value="ENSXCOP00000021680.1"/>
        <property type="gene ID" value="ENSXCOG00000016210.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157350"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.20:FF:000002">
        <property type="entry name" value="helicase POLQ-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001293">
        <property type="entry name" value="helicase POLQ-like isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000058">
        <property type="entry name" value="Helicase, POLQ like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046931">
        <property type="entry name" value="HTH_61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048960">
        <property type="entry name" value="POLQ-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF12">
        <property type="entry name" value="HELICASE POLQ-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20470">
        <property type="entry name" value="HTH_61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21099">
        <property type="entry name" value="POLQ_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="347"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="67"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="872" mass="97804" checksum="88EEC47B71656714" modified="2018-12-05" version="1">SILAKLEEVEQHERLRSENLQSFRTLQPSADPMSQSPKGPLTDSVFHELEDKVFEDLPSSQLQFQEQIQDRAKKSKLQVRNKTSTPSHTEAAGETNSEDGSRRHSKARRSVADLLKKTMLGNAVTPVSVSRTALLKEAVVSEEISVAMQAMETISAETLDLGPFFGLPSKVKELMHKLRGIQKLYDWQEACLNLECVQQRRNLIYSLPTSGGKTLVAEILILRELLCRRKDCLFILPYISLVQEKLHMLGDGSRGAVIEMTLAKVRHMSKATQIIGMSATLGNIKDLQTFLGAENYTNNFRPVQLKEYVKLNDTIYEVDPKEENCFRFSRMLNFKYSSSMQKIDPDHIIALVTEVIPLHSCLVFCPTKKNCENVAGMICKYLKEDFLQHREAEKAVLLQELKAGGNGSMCPVLRRTVPYGVAYHHSGLTAEERKLVEEAYSRGVLCLLACTSTLAAGINLPARRVILRSPYVATDFLKRSQYKQMVGRAGRAGIDTIGESILILQEKDRKLVCAPMESCYSNLLQDDGKGILSLILSLIGLNISSSLQQVEQFLRGTLLFVQQQQLCVERSLWDTVQRCVATLQEKELITNTATHSPSLQVTRLGRATYKGSVDLSYCSVLYKDLLKGLEGLQLSSHLHLVYLVTPYDLVPQCNPDWMVYFRQFTLLSASEQKMSAAVGVPESFVARKAAGQTVKKSVDLVAVKRMYLSLVLFSLLRDTNVWAVADRFQLSRGFVQTLLSAASTFCCCVLHFTEELEELWPFKALLLELTRRLSYCVKAELIPLMEVAGVLEFRAKQLYNAGYRTLTHLANADPAVLCGTLEHLYQKQANLMVASAKMLLNEKAAALQEEVDELLTLPADLPQQQHQELLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEW6</accession>
    <name>A0A3B5MEW6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Solute carrier family 6 member 4b</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEW6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022828"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023104.1">
        <property type="protein sequence ID" value="ENSXCOP00000022828.1"/>
        <property type="gene ID" value="ENSXCOG00000017058.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164250"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051378">
        <property type="term" value="F:serotonin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005335">
        <property type="term" value="F:serotonin:sodium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF129">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="274"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="325"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="376"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="407"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="511" mass="57549" checksum="A829CAF507B4BB82" modified="2018-12-05" version="1">MLSFFLSLFSFLSSFLSTLKRLLSCMCSGAFLIPYILMAIFGGVPLFYMELALGQFHRTGAISIWKHICPIFKGIGYAICVIALYVSFYYNTIIAWALFYFYSSFSSILPWTNCDNVWNTPNCTNYFGMDNVTWTNSSSNRNHICENVLEIHKSSGLRDVGGVRWQLMLCLFLIFTIVYFSLWKGVRTSGKVVWVTATLPYIVLFILLIRGATLPGAWRGVVFYLKPQWDKLLETSVWVDAAAQIFFSLGPGFGVLLALSSYNPFTNNCYRDAIVTSLVNCLTSFVSGFVIFTVLGYMAEMRKVEVEDVAKDKGPSLLFITYPEAIANMMGSTFFAIIFFVMMITLGLDSTFGGLEAIITAVLDEYPDYFSHRRELFVLCLVVVCFLGSLSTLTNGGAYVVKLLEEFGVGCSIIAVGFLEAIAVSWFYGIKRFSNDVQAMLGQAPGLFWRVCWVAISPAFLAVREENKVKIFITVAYTSDWHNENTLTRIYFEGKKCLICLLFVCIRLSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFS9</accession>
    <name>A0A3B5MFS9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Prominin 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Microvillus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prominin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MFS9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019976"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020223.1">
        <property type="protein sequence ID" value="ENSXCOP00000019976.1"/>
        <property type="gene ID" value="ENSXCOG00000015001.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063586"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031528">
        <property type="term" value="C:microvillus membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071914">
        <property type="term" value="C:prominosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008795">
        <property type="entry name" value="Prominin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730">
        <property type="entry name" value="PROMININ PROM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22730:SF3">
        <property type="entry name" value="PROMININ-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05478">
        <property type="entry name" value="Prominin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017323798" evidence="8">
        <location>
            <begin position="18"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="419"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="469"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="782"/>
            <end position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="848" mass="95023" checksum="612FE7179CE6A255" modified="2018-12-05" version="1">MWKSGTLLLLSVLWASGELQQETEPRRLPPPGKLDFGYVPAGVYETLAHYEPGPIGILFHLVHAFLHAVQPNAFPHDLIVKLAKDKFGAIQTEYQKAIYYEIGFLICAAVGLLFAILMPVVGLFFCMCRCCDNCGGEMHQRQRKNADCRRGLLGTLLFSTSLVITIGVLCAYAANQNLSSQVKNIRRLVNSNMRDLHTFANDTPMVRQNKNNINNIGPLLGGRIHVRLDKEVHPSLNQVLNMAGAMRETKEALENVSVSLVVLQEGAGKLHFNLSQVCDSINRTLDDPGCHDEESDATTAQLCRSIRQSLSQLQISANFTRLPNVNPQLEKMKNVVKTDLSSVVHRGFSSLNDTPHIVIEQTRSVVNSVQNLVDSVGNNISSFSKAFPLQTCLSNFTIFISHAHAKIEDYYPEVDKMDFYRWIGCIALCCMVVLVLAFNYLGLLCGTLGYDKHASPTTRGCISNTGGTLLMAGVGFSFIFSWMLMGVLTTIFLAGGNMEKLLCEPFHTKELFKVVDTPYLINPEWKNFIPGYMYNDSDLELTSESLYSTCKQNKGIYSALRLDKVFNISSFLNTTVFTKDVVRQLENFRIDLRTIILLEAEGKQNLLDFSEAGLSEINYADYLDEVNKGVTVVDVLSFAKELESQTDLMVRKGPLQTALKGHVATLQRIHRQQITPMEQAMSALNQSMRFLERTASDLPNKVTDVLEAIEAAQHLISQNATLLINQETRKYSATIIGYFNQYIEWVKTSLALEVAPCKPFSNILDTAQIMTCSFLVDSMNTFWMGLGCSTLFLLPSVILAVKLAKYYRRMDTEDVYDDVETIPMKNVPTYDTMNRFPRASAPPRHIDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MH31</accession>
    <name>A0A3B5MH31_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">POPDC1-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the popeye family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027886213.1">
        <property type="nucleotide sequence ID" value="XM_028030412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MH31"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023062"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023339.1">
        <property type="protein sequence ID" value="ENSXCOP00000023062.1"/>
        <property type="gene ID" value="ENSXCOG00000017234.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114152504"/>
    <dbReference type="KEGG" id="xco:114152504"/>
    <dbReference type="CTD" id="64208"/>
    <dbReference type="GeneTree" id="ENSGT00390000002563"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007519">
        <property type="term" value="P:skeletal muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051146">
        <property type="term" value="P:striated muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006916">
        <property type="entry name" value="POPDC1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055272">
        <property type="entry name" value="POPDC1-3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12101">
        <property type="entry name" value="POPEYE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12101:SF18">
        <property type="entry name" value="POPEYE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04831">
        <property type="entry name" value="POPDC1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="POPDC1-3" evidence="7">
        <location>
            <begin position="31"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="297" mass="33899" checksum="94022A15E4970ABB" modified="2018-12-05" version="1">MEPPDFEAMNLTVKPAPPAFPLCGEWKEAAEGSLFHLANILLFLGFMGGSGFYGHLYLFTFLTVGFFCSTVWAWSDPCTTDTFLWNFALFGACLSQALYIVYKLRSLTFDKDFQELYNCMFKRLGVSLPHFGKIVTSSDGVVHTLEKGQFFAVEGKTAIDKLSVLLSGRIHVTVNGEFLHYIYPFQFLDSPEWDSLRPSEEGIFQVTLHADDQCRYIAWRRKKLYLLFAKHRYIARIFALIVRSDIAEKVYSLSDKAFDMAGHRYDLRLPSYCHMPGPELERAHDLLEVPVPGERAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJA2</accession>
    <name>A0A3B5MJA2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rap guanine nucleotide exchange factor 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Cyclic nucleotide ras GEF</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Neural RAP guanine nucleotide exchange protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">PDZ domain-containing guanine nucleotide exchange factor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">RA-GEF-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Ras/Rap1-associating GEF-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">RAPGEF2</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Late endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RAPGEF2 family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000019629"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019874.1">
        <property type="protein sequence ID" value="ENSXCOP00000019629.1"/>
        <property type="gene ID" value="ENSXCOG00000014741.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156418"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070161">
        <property type="term" value="C:anchoring junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06755">
        <property type="entry name" value="PDZ_RapGEF2_RapGEF6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01785">
        <property type="entry name" value="RA_PDZ-GEF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45161">
        <property type="entry name" value="CYTOSKELETON-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45161:SF2">
        <property type="entry name" value="RAP GUANINE NUCLEOTIDE EXCHANGE FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0965">Cell junction</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="14" id="KW-0221">Differentiation</keyword>
    <keyword evidence="13" id="KW-0967">Endosome</keyword>
    <keyword evidence="7" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="12 24" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="18" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="27">
        <location>
            <begin position="131"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="30">
        <location>
            <begin position="263"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="28">
        <location>
            <begin position="381"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="29">
        <location>
            <begin position="612"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="26">
        <location>
            <begin position="723"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="36"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="476"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1010"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1083"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1206"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1286"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="25">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="476"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="1102"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="1133"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="1206"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="1231"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="1286"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="1320"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="1385"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="25">
        <location>
            <begin position="1405"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="25">
        <location>
            <begin position="1421"/>
            <end position="1432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00029925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00031545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00031980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00032021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1455" mass="161243" checksum="B8B92895FADA2CEA" modified="2025-10-08" version="2">MNPLAVPILNSWYGKLLPADFSRLHLADGLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATENEPGDMDLSGLPETAVDSEEDDDEEDIERASDPLMSRDIVRDCLEKDPMDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDSRTEMLCMGNSFGVSPTMEKEFMKGVMRTKVDDCQFVCIAQQDYCCILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTPERLTMHLVEEHSVVDPTYIEDFLLTYRTFLSSPMVVGKKLLEWFHDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTHFLEEFESNLEKEKMCGHLRLLNIACAAKAKPRLVTLTKPSRDSPLAFTLLGGQEKGFCIFIDTVEPGSKAAEAGLKRGDQILEVNGQNFETVQFSKANEILKNNTHLSITVKTNLLVFKELLTRPEHEHELDGDEEHDRKNGAPHLPKIGDIKKGSRYSIPDLAVDVEQVMGLEKASKKAKSNTVGGRNKLKKIFDKTLTSILPPKPYNDVCVGQSQDDSIVGMKPSKQIPAALPVSGNLSSSNPDLLQSHHRILDFNNQPDMSDQVLRVFKADQQSRYIMIGKDTTAKEVVAQAIREFALTATPEAYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKSNMETETLCSDEDAQDLLREGQIALLQLSTVEVATQLSMRAFELFCAIEPTEYIDDLFKLRSKTGSVSLKRFEESINHETFWVATEVVREPNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVSRLRGTWEKLPSKYEKLFGDLQDLFDPSRNMAKYRNVLNNQNLQPPIIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSANAAVLDVSQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLSLETNEESLQTFSLQCEPSISTLPKNAGGKRPDTSPVVPRAASQQRGQLAKGNQVLQVPSVALYPSRKRVPVKDLPPFGTSSPQSLKKILSLSEEGNERHRRQPEDTMSNASSQLSSPPTSPQSSPKKGYSRSGDAYSDSGHSEISSRSSLVSNSSFDMAQEERRLRHSGGAGESHMGGPRLERRATTDPDQYSLGSYSSMQDCRGIYAGCSTVLSSPSSEELTQDQGDRVSLDAADSGRGSWTSCSSGSHDNIQNMQQGRSWELGIGGLSSASEALLGGPAALWAAQARGSWASASSSSSSAAYWGEESEGDTGTIKRRGGKDVNADAETSSITSTGSEEAKQLGRPSPSPITAGARKEGRYREPPPTPPGYTALTLSDLAEGQHPRRPPDYTTALQRSRMVTQSPDSHQAHQAAKHRGGSLHRTRSPADEQEPDDEEEGESLSPKLVALRKPLARHTPEAPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MK21</accession>
    <name>A0A3B5MK21_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MK21"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024219.1">
        <property type="protein sequence ID" value="ENSXCOP00000023935.1"/>
        <property type="gene ID" value="ENSXCOG00000017871.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154308"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001480">
        <property type="entry name" value="Si:cabz01071911.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001732">
        <property type="entry name" value="Zgc:162936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000557">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001506">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000625">
        <property type="entry name" value="Zinc finger protein 536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 208-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="206"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="234"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="262"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="290"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="318"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="346"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="430"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="459"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="482" mass="54406" checksum="B2C01CE416C59E3E" modified="2018-12-05" version="1">MSSTVSIHTQLAAVMEALVHAAVAELERLEEDRELSKARGTTEERHETGRVQKETREKLARFASIMETLGNEALGRILNIMDEARLVAQVGHVCKKPQTSVLNVLSHTSVGMNTQAPNNVTHQSKEMESYDVNVVICSQQSLTVSVKDGHGNIDLEALPSEFSVLSETSQQNVSQKLFSCSVCGKFFSSQSNLKSHQLIHTGEKPFACSICSRAFRQRQSMQSHMRTHTGERPFQCPVCGKRFSKQAQLKTHTVIHTGEKPYGCDVCGRCFNLPQNLHRHSVTHSREKVFICSDCGKGFTRAVTLKTHQLIHSGQKPFKCEQCPKLFRHAVNLKNHQRIHSDLRPYRCDLCGKTFRQAVNLKIHGRIHTGERPFSCQQCGKMFSQQSSLISHSRTHSTDRPFQCPTCDKKFNNANSLKLHLRVHTGEKPYACEICGKTFSQGSHLRTHKRHVHAGGKQFICDKCGKRYADQRYLKLHKCCYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKA5</accession>
    <name>A0A3B5MKA5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BicC family RNA binding protein 1a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKA5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019949"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020196.1">
        <property type="protein sequence ID" value="ENSXCOP00000019949.1"/>
        <property type="gene ID" value="ENSXCOG00000014979.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156276"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627:SF78">
        <property type="entry name" value="PROTEIN BICAUDAL C HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10627">
        <property type="entry name" value="SCP160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="144"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="230"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="326"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="240"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="431" mass="45910" checksum="298E866BAA494C1B" modified="2018-12-05" version="1">MALRFDGTTVDLTLFLCLVVIATGFLGATAPHSLPASATLNTVLNSLNSALSPLQTPSPSTPTPLLKKPFFPQMFFELVLHPAAQALLMSGVQGYTLSTPSPPPGLAPIDKQPNGVPECTLNGHVKHPGYGSLATASLTETVLSPTPCDSAHEASGHSPSEPLSSKSNPDEGSDTFVEVGMPRSPSHSANGSELKQMLASCKTSPGKRQAVEFLQGTKNSYLHSDCLLSDAESSSSDGPVADKRAPGSERAAERAAQQNERERIRLAPQTSFANMQAFDYEQKKLLATKAMLKKPVVTEVRTPTNTWSGLGFSKSMPAETIKELRRANHVPYKPSMSTTYEGSPLSLSRSGSREGVGNGSDSDNWRERNGNGLPNHTEFPSAVSSPKRKQNKSGQRAVLGGGPARYILLYIVYIITPCSLIPGFHLMLTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLP2</accession>
    <name>A0A3B5MLP2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLP2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020409"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020657.1">
        <property type="protein sequence ID" value="ENSXCOP00000020409.1"/>
        <property type="gene ID" value="ENSXCOG00000015292.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155860"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017134">
        <property type="term" value="F:fibroblast growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005007">
        <property type="term" value="F:fibroblast growth factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052615">
        <property type="entry name" value="FGFRL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19890">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19890:SF10">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR RECEPTOR-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="30"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="249"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="417" mass="46064" checksum="D5BF8A6F310AE6CF" modified="2025-10-08" version="2">MCLTVRQKSKLLTKISLPLCRVAERSPHRPILQAGLPANRTAVVGTNVEFECKVFSDPQPHIQWLKHIEVNGSRVGPDGLPYVRILKTAGVNTTDKEMEVLQLRNVSFDDAGEYTCLAGNSIGFSHNSAWLTVLEAVTPYPPSSNTYLEVVIYCVGFFLIAIMITIVIILKIRASSKKSDFNSQLAVHKLAKSIPLRRQVSVDSSSSIHSGVMLVRPSRLSSSGTPMLTGVSEYELPQDPRWEIPRDKLVLGKPLGEGCFGQVMMGEVLGLDKEKPNRVTKVAVKMLKSDATEKDLSDLISEMEMMKIIGKHKNIINLLGACTQDGRRRDEQGADTNLTRLVHTHTHTHTHTHTHTVVPQTRVLLHYLFSLYLPGLDAVFHSPVFGDLSTCANPHKSVFAPAGIYMQTVASRSISPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MMG7</accession>
    <name>A0A3B5MMG7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nephrocystin 3-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MMG7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020664"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020915.1">
        <property type="protein sequence ID" value="ENSXCOP00000020664.1"/>
        <property type="gene ID" value="ENSXCOG00000015485.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155116"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056884">
        <property type="entry name" value="NPHP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24883">
        <property type="entry name" value="NPHP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Nephrocystin 3-like N-terminal" evidence="2">
        <location>
            <begin position="53"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="236" mass="25789" checksum="DD6DED3389F36BC6" modified="2018-12-05" version="1">MAQTSLLQGKRFYCREWVFHKIQHCLQEKTNNLSGVSSTHSKQPLPSGSSWGVLLVGGPGSGKTALCTELLWPTSAQGTYRSLHKQSLAFHFCRADDSDTLCVGGFIRGLVAQICRGGLVPGYEEKVRDPAVQSTLQPGECERNPAEAFKKCVLLPLLSVKPPQQSLFLLVDSIDEGSQLGEGEQRSSPGSPRTIAELLASHHEFLPPWLLLICSARRQNKAITKLFTGTLPSSVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MMI7</accession>
    <name>A0A3B5MMI7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MMI7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020935.1">
        <property type="protein sequence ID" value="ENSXCOP00000020684.1"/>
        <property type="gene ID" value="ENSXCOG00000015504.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000181570"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022111">
        <property type="entry name" value="Rhodanese_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12368">
        <property type="entry name" value="Rhodanese_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanase C-terminal" evidence="1">
        <location>
            <begin position="11"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="96" mass="10765" checksum="D8D72B65EDFB88CA" modified="2018-12-05" version="1">FLFPSGPVHSMYCGLPWDQYELCSTQFCCQLVLSCPSCRKKGCTACCPSCQRKGETQDKEASDVQHPKEECECTDTRARIPQDNCGPVIMQKPQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQ80</accession>
    <name>A0A3B5MQ80_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQ80"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021644"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021908.1">
        <property type="protein sequence ID" value="ENSXCOP00000021644.1"/>
        <property type="gene ID" value="ENSXCOG00000016183.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154817"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045180">
        <property type="term" value="C:basal cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043515">
        <property type="term" value="F:kinetochore binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040001">
        <property type="term" value="P:establishment of mitotic spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019399">
        <property type="entry name" value="Parkin_co-regulated_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF28">
        <property type="entry name" value="CLIP-ASSOCIATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10274">
        <property type="entry name" value="ParcG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="13583" checksum="DA5584EEB406F515" modified="2018-12-05" version="1">MAGSIHPEQCIKVLCPIVQTADYPINLAAIKMQTKVIERIAKDSLLQLLPDIIPGLLQGYDNTESSVRKASVFCLVAIYAVIGEELKPHLAQLTGSKMKLLNLYIKRAQTTNSNSSSSSDVSSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQF1</accession>
    <name>A0A3B5MQF1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ankyrin repeat and IBR domain-containing protein 1</fullName>
            <ecNumber evidence="4">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RBR family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQF1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021719"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021983.1">
        <property type="protein sequence ID" value="ENSXCOP00000021719.1"/>
        <property type="gene ID" value="ENSXCOG00000016241.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157621"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20346">
        <property type="entry name" value="BRcat_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20361">
        <property type="entry name" value="Rcat_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16774">
        <property type="entry name" value="RING-HC_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000003">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000040">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000129">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045840">
        <property type="entry name" value="Ariadne"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047564">
        <property type="entry name" value="Rcat_RBR_ANKIB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047563">
        <property type="entry name" value="RING-HC_RBR_ANKIB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19422">
        <property type="entry name" value="Ariadne"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="12 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="45"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="145"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="330"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="334"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="293"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="980"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1014"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="569"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="304"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1062" mass="119467" checksum="B8643E8DC8880319" modified="2018-12-05" version="1">MGNTATKFRKALINGDELLACQLYESNPQFKEALDPNATYGESYQHNTPLHYAARHAMMRLLRSFLINKDGNPNKRNVHNETSLHRLCMGPQILTSEGALQPRISRPYEDEQRRMECLHIILSWTGAKLDHGEYESAEINATDNKKNTCLHYAAASGMRTSVELLLQRGADLFVENENRETPCDCAEKQHHKDLALSLESQMVFSLAPEAEGIEADYAALDRRELYEGLRPQDLRRLKDMLIVETADMLQAPLFTAEALLRAHDWDREKLLEAWMSNAEECCQRSGVQMPNPPPSGYNAWDTLPSPRTPRTTRSSITSPDQISLLAGDDDSSLCGICMSSVSFFEEPVDMSCGHDFCRACWEGFLNLKIQEGEAHNIFCPAYDCYQLVPVEVIESVVSREMDRRYLQFDIKAFVENNPAIHWCPVAGCERAVRLNAQGPGTSNADPLSFPLLRAPAVDCGKGHLFCWSCRGEAHEPCDCDTWKLWLQKVTEMKPEELAGVSEAYEDAANCLWLLSNSKPCPTCKSPIQKNEGCNHMQCAKCKYDFCWICLEEWKKHSSSTGGYYRCTRYEVIQQVVEQSKEMTEEAEKKHKSFQELDRFMHYYTRFKNHEHSYQLEQRLLKTAKEKMEQLSRALSGREGGPPDTTFIEDAVLELLKTRRILKCSYPYGFFLEPKSTKKEIYELMQTDLEMVTEDLAQKVNRPYLRTPRHKIIRAACLVQQKRQEFLASVARGVAPNDSPEAPRRSFAGGTWDWEYLGFASPEVKECLFKIPLLKNILVLLNNKFSQIMCIPLIHIIEPYHRTVYGLDSLDEDDPNILLAIQLSLQDSRMTEIGSNHDALANEASLGAIGTSLPSRLEQSALGVDVLPRASLSSSELLERADNLARLGNISGQYSPDSTTCGSNEPSSSSALTTNANLLGNIMAWFHDMNPQGITLIPPTDPNVAPPPEDREGCSQDNEKGGFIVFPDDRKPQEAMADAGFCTQRSSNTEEKESAVAERPTQLDLVGLDTMQLPYTAAPDRSGEHGECASSKGCHVDSPRSDYASDQLPSTSSSEWEDHMHLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQG1</accession>
    <name>A0A3B5MQG1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transmembrane and ubiquitin-like domain containing 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQG1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023416"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023697.1">
        <property type="protein sequence ID" value="ENSXCOP00000023416.1"/>
        <property type="gene ID" value="ENSXCOG00000017481.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014069"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040352">
        <property type="entry name" value="TMUB1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14557">
        <property type="entry name" value="PROTEIN C7ORF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14557:SF4">
        <property type="entry name" value="TRANSMEMBRANE AND UBIQUITIN-LIKE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="159"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="68"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="122"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="88"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="133"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="314" mass="33396" checksum="72AF15326E9555D1" modified="2018-12-05" version="1">MAVCALTMLEGVEEEVVATGGALLLALALILAWLSTHVTDRGDHILGTILTVGAHASLIGLGAHDNYSGGPNGADAPEQQAAQPAQENKPDDGEPANERGEGDAEAAEDVWTDLRLDIRSKQPQAGALHSSDGEEDEADENDDEEEETTVPCASTTTSISVRLKFLNDTEEVAVVEPEDTVGILKSKCFSGREHQVKLIYRGQLLQDPKKTLFSLNITDNSVIHCHISQAVQGPNPEEGSQPGAGAGPVASGGFRSASVAISTSSLVVPVFVVILAVVWYFRINYRQFFTAPATISLVGVTVFFSFLIFGMHSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MR44</accession>
    <name>A0A3B5MR44_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proline-rich nuclear receptor coactivator 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MR44"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026258"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026576.1">
        <property type="protein sequence ID" value="ENSXCOP00000026258.1"/>
        <property type="gene ID" value="ENSXCOG00000019612.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063881"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016071">
        <property type="term" value="P:mRNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028322">
        <property type="entry name" value="PNRC-like_rgn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026780">
        <property type="entry name" value="PNRC1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15405">
        <property type="entry name" value="PROLINE-RICH NUCLEAR RECEPTOR COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15365">
        <property type="entry name" value="PNRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="124"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="129"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="18601" checksum="4D76407099AF9CCA" modified="2018-12-05" version="1">AAEEGRQEAAAASPETPETLPEHPAVRPQQHRLRRHPDGLVGAPRHLPRHDQLAHNVNTRSHKPKQSQSPVASPPAKKNDNRTPKKPSSSVEPKKPLNASNNVTFPTGSSAEAEYLKDCEKIYAGPKFSEPPSPSVLPKPPSHWVGEDGQNSQSHEQMSVHLKSLLKVQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRB0</accession>
    <name>A0A3B5MRB0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Vitamin D receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. Recruited to promoters via its interaction with BAZ1B/WSTF which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homodimer in the absence of bound vitamin D3. Heterodimer with RXRA after vitamin D3 binding. Interacts with ncoa1 and possibly other coactivators, leading to a strong increase of transcription of target genes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the nuclear hormone receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027858442.1">
        <property type="nucleotide sequence ID" value="XM_028002641.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027858443.1">
        <property type="nucleotide sequence ID" value="XM_028002642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRB0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023731"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024015.1">
        <property type="protein sequence ID" value="ENSXCOP00000023731.1"/>
        <property type="gene ID" value="ENSXCOG00000017721.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114135384"/>
    <dbReference type="KEGG" id="xco:114135384"/>
    <dbReference type="GeneTree" id="ENSGT00940000155473"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:nuclear receptor activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0070644">
        <property type="term" value="F:vitamin D response element binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0003146">
        <property type="term" value="P:heart jogging"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:heart looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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    </dbReference>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="NR_DBD_VDR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Vitamin D3 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000021">
        <property type="entry name" value="Vitamin D3 receptor B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DBD_VDR"/>
    </dbReference>
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        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
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        <property type="entry name" value="Nuclear_hormone_rcpt_NR1"/>
    </dbReference>
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        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
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        <property type="entry name" value="VitD_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24082:SF504">
        <property type="entry name" value="VITAMIN D3 RECEPTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00350">
        <property type="entry name" value="VITAMINDR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="16">
        <location>
            <begin position="21"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="17">
        <location>
            <begin position="131"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="106"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00063971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="48163" checksum="490CCFFC89CEF0C6" modified="2018-12-05" version="1">MEPTVVSTSTVAPDEFDRNVPRICGVCGDKATGFHFNAMTCEGCKGFFRRSMKRKASFTCPFNGSCTITKDNRRHCQACRLKRCVDIGMMKEFILTDEEVQRKKDLIQRRKDEEAQREAEKEARRPRLSDEQSHVIATLVEAHHKTYDDSYSDFCRFRPPVREGPVTRSASRAASLHSFSDASSDSFSHSPESVDTKMNFNNLLMMYQEHGNSPDSSEEEGSSYSMLPHLADLVSYSIQKVIGFAKMIPGFRELTAEDQIALLKSSAIEVIMLRSNQSFNLEDMSWSCGGPDFKYQISDVTKAGHTLELLEPLVKFQVGLKKLNLHEEEHVLLMAICLLSPDRPGVQDHSRIEALQDKLSETLQAYIRLHHPGGQLLYAKMIQKLADLRSLNEEHSKQYRSLSFQPEHSMQLTPLVLEVFGSEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSX0</accession>
    <name>A0A3B5MSX0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Si:dkeyp-34f6.4</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSX0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027197.1">
        <property type="protein sequence ID" value="ENSXCOP00000026868.1"/>
        <property type="gene ID" value="ENSXCOG00000020067.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177780"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="85" mass="8771" checksum="9F32CEA23AF710DC" modified="2018-12-05" version="1">ICFSSLFRLFLVLVWSGGGVALAPVLVVVGGLLPLHVLHFLAVKVVIARKLHALQALLSGLGMSLYVLAAGEQLAADVAAEGAHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSY9</accession>
    <name>A0A3B5MSY9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ras-GEF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSY9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024326"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024619.1">
        <property type="protein sequence ID" value="ENSXCOP00000024326.1"/>
        <property type="gene ID" value="ENSXCOG00000018170.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160144"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF230">
        <property type="entry name" value="RAP GUANINE NUCLEOTIDE EXCHANGE FACTOR-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GEF" evidence="3">
        <location>
            <begin position="319"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="557" mass="63935" checksum="A2E7DB6FF41DD2EB" modified="2018-12-05" version="1">MPAGMKPLEKFLKKQTTALTRAGGFGGVGDKASGGTGASRRRASLSRVVPFFRETSPESGHAREVFTLLDDFMLTHPIFLAADRLQQVLLQGGSERRDGEAEAAMSAAERKQAVLNMAFRYLDTYRELLQEEGERSNSFPKELYMCAVQELTRYPELVEDVLKLQRWSEILHCSSDEEKESRKKQVRPLFRHFRRIDACLQPREAFRGSDEFCRVYTPDHSYVTIRSRLSCRVGEILALVKEKLQYSEDQPVLPGNLILVAVTSAGKAVFRPSDEAVFTTLGVNTHLFACEPSELDSLQLPLPEEIHWTPGDSKLHDMSAEEVANQLVVFDWELFSCVHEQVEFICYVFHGEQSRWRPLNLELVLQRCSEVQHWVATEILQCQSLPKRIQLLRKFIKIAACKQQQDLLSFLAVVLGLDNPAVSRLRLTWEGLPGKFRKQFQQFESIADPSRNHKSYRDLITSLRPPLIPFTPLLLKLTFLHESCKTFHGEFVNFEKMLQHKVAEMVRTIRRYRSSQLIDTETSPSHLQTKAYVRQLQVIDNQNLLFDMSCKMEPKDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVY8</accession>
    <name>A0A3B5MVY8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FA complementation group B</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVY8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025497"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025804.1">
        <property type="protein sequence ID" value="ENSXCOP00000025497.1"/>
        <property type="gene ID" value="ENSXCOG00000019049.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009885"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043240">
        <property type="term" value="C:Fanconi anaemia nuclear complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000042">
        <property type="term" value="P:negative regulation of double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905168">
        <property type="term" value="P:positive regulation of double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033333">
        <property type="entry name" value="FANCB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28450">
        <property type="entry name" value="FANCONI ANEMIA GROUP B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28450:SF1">
        <property type="entry name" value="FANCONI ANEMIA GROUP B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="433"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="436"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="829" mass="91635" checksum="9A121FD71F6B3C1C" modified="2018-12-05" version="1">MEKLSSDDSNRKVHGLSHGGKIILFACKRDPAVNVSERGGLTFRSFYFEREGNAFLNATEGATAISRKLSAHVDIVTCKCAVDVQKRVKTAFVLVTKKSEKGGSFKYSLLALRSSHRLEPCIEFKLPYQMKGKVCILQGPTVLWSHDNSVSYTSPLAEGVQKIPIQMSKCLFGELPIQKGQLFALGLSEPLLANHSPTLGYLFEGGHAFDGSLILPHPYISITQCMRVLTADRVDDVLRCSVIAATSSKQLVLFENGTVKDTCEVPFGQVEDIQMVDTGRNGCLFVVSFKQGHVCAVWKETFQIASCWSDVDSTHVDDFLGCGTEQILLIFRNKGISSGPLEKFLITDLCGISYSCGQGSEVPKASPPPQENSLLTLQALESRLQSGLIVLQELEREVRVKDRVLQQSIQVLTDVLSGRETVLSQPEQEGLVALWDSDEESNDETLDDKSHESPALSSRPKIDKLWHRITEEQPASGVSLSLLTEPGQNSTPAVIQTRSQVFWLPACGPSTPTLSSPSSGFTFSEPAAKRSRQHIASDDFSGCRLAVTAVTKLAPLLTSGCVKCHAMLHYVEKAEAVFPGSSPTTAVLHCGEVSLDIHDLCQKPLLQNPQIKTDEVREDLLSLLTVLDNWVLRIDSPDYSLGDIDGWIKRREGFKKINVSPEYLLLDSPGSSAPMLLRWHQINPFQGDLSIHSSQLQMLQFLDSMLSYLPDSCFLQPVKRARSQSTAQKLASALEKEVLSLREGLSSLLCVKDEEERNKGHTEHCEIPDSGSAEDLIRCCREVWQQNVEKSRLRLNPVVDAGRYREMIQSISQVKMESDLAALRQLYQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVZ2</accession>
    <name>A0A3B5MVZ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">small monomeric GTPase</fullName>
            <ecNumber evidence="5">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">RAB9A</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Phagosome membrane</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVZ2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025502"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025809.1">
        <property type="protein sequence ID" value="ENSXCOP00000025502.1"/>
        <property type="gene ID" value="ENSXCOG00000019054.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158619"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032482">
        <property type="term" value="P:Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04116">
        <property type="entry name" value="Rab9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000360">
        <property type="entry name" value="RAB9B, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041824">
        <property type="entry name" value="Rab9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF9">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB-9A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="220" mass="24547" checksum="B5CA3DBE05ECF512" modified="2018-12-05" version="1">TSPTVSRAGSVTPAVTHLSLKGKSLLKVILLGDGGVGKSSIMNRYVTNKFDAHLFHTIGVEFLNKTLEVDGNQVTLQIWDTAGQERFRSLRTPFYRGSDCCLLTFSVDDNQSFLNLSNWKKEFIYYADVKEPENFPFVVLGNKVDVTERQVSTEEAQMWCQENGDYPYFETSAKDATNVAVAFEEAVHRALAADDKADHLIPTDTVKLHRKPRSATSCCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MW31</accession>
    <name>A0A3B5MW31_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LAP (LRR and PDZ) protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MW31"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023804"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024088.1">
        <property type="protein sequence ID" value="ENSXCOP00000023804.1"/>
        <property type="gene ID" value="ENSXCOG00000017763.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159526"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
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        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
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        <property type="term" value="P:neurotransmitter receptor transport postsynaptic membrane to endosome"/>
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        <property type="entry name" value="Erbin isoform 7"/>
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        <property type="entry name" value="Leucine-rich repeat-containing protein 7"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
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        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
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        <property type="entry name" value="LRR_dom_sf"/>
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        <property type="entry name" value="PDZ"/>
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        <property type="entry name" value="PDZ_sf"/>
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        <property type="entry name" value="Synaptic_Scaffolding_LAP-MAGUK"/>
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        <property type="entry name" value="DISCS LARGE"/>
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        <property type="entry name" value="ERBB2 INTERACTING PROTEIN"/>
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        <property type="entry name" value="Outer arm dynein light chain 1"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0677">Repeat</keyword>
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        <location>
            <begin position="976"/>
            <end position="1065"/>
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    <sequence length="1067" mass="117764" checksum="BD1A80EEFE15EB96" modified="2018-12-05" version="1">MHGMKASLLKSHISATGQCNVIHTMKNNKTTNKQSWNADKMTDAPGDRRPTLLTSIDELDCSFNEIEALPASIGQCVSIRTFAADHNFLSQLPPELGSWKSATVLFLHSNKLESLPEEMGDMQKLKVINLSNNKLKNLPYSFTKLNEMTAMWLSENQSKPLIPLQKEEDPETHKTVLTNYMFPQHAEDCEFIPSSDSENFNPALWEEQRKQRAQVAFECDEDKDERETPPREGHLKRYPTPYPDELKNMVKTAQSVAHRLKEDETSDESGKESKPVERNHIGVQDVGVKVSSTCYWKKVFKYAFVFFYELSEEEEMKIAEMRPPLIEISINQPKVVTLSKDKKDDADSLLDDTVANSNQNNSNCSSPSRMSDSVSLTTDSSQDNSLCTPERESKIQDDENMNQPKDTTPLLHNGNGSEPLLQTLLKTQQTPPDYDLSMEAYIEKGVTNGMGDAYTKWDQINMNVTKLPPYHAAQFNKLDKVKNGSVNDCNDFQNGNQQGSDCINSLVNRSQGTRAETSASSGVAASSDMSLSRSTEELSPEKRCPLPSQVTKSHSVSNIEAGGMKLYSFDGDDEAAAAAAGRIVPGPGAQGQNIVRSKSASQLLNDQNLLVYPGSLSSSSDLLSSSKPPVSTSRYLGSSSMSMGVPPPQYNIQYASSAVPKDDLWAQRDHQGYLPPHPHSLANTNYSNRNQAPPYTLQPQQRGVPMAPMGDWTKERLHSAGGPPRSSTLQRQGSSASSVSMGDPKRVQAPEGEYMTYRDIHTLARGPLAMSQAMQRPFSARTYSIDAPIASRPVAARPPPHELPERTMSVSDFSYQQSSPSKRPNTRVKSEHSLLDGPGQVSGGMGPGRVPTDWRDQVMRHIEAKKMEKNALSRSYNFNTSPLSWSHYGSCRDMHAGQGGVAFSGADRQPYGALSFRVSTVPLMNGQMGPSGRPQMSQSSMARHPSREQLIDYLMLKVSQQPHGPPRVPHDTLQQEIRVKVEKNPELGFSISGGVGGRGNPFRPEDNGIFVTRVQPEGPASKILQPGDKIIQANGYSFVNIDHGNAVSLLKMFPTTVDLTIVREVQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWB5</accession>
    <name>A0A3B5MWB5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sorting nexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sorting nexin family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_028036559.1"/>
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    <dbReference type="STRING" id="32473.ENSXCOP00000027966"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028306.1">
        <property type="protein sequence ID" value="ENSXCOP00000027966.1"/>
        <property type="gene ID" value="ENSXCOG00000020863.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="xco:114156236"/>
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    <dbReference type="GeneTree" id="ENSGT00940000160162"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:cleavage furrow formation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="P:mitotic cytokinesis"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:plasma membrane tubulation"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PX_dom"/>
    </dbReference>
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        <property type="entry name" value="PX_dom_sf"/>
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        <property type="entry name" value="SH3-like_dom_sf"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR037427">
        <property type="entry name" value="SNX33_BAR"/>
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        <property type="entry name" value="Snx9_fam"/>
    </dbReference>
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        <property type="entry name" value="Sorting_nexin_WASP-bd-dom"/>
    </dbReference>
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        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45827:SF3">
        <property type="entry name" value="SORTING NEXIN-33"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BAR_3_WASP_bdg"/>
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    </dbReference>
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        <property type="entry name" value="PX"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Snx9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="12">
        <location>
            <begin position="218"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR027744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR027744-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="562" mass="64693" checksum="209BC113B2A53894" modified="2018-12-05" version="1">MSVKGVAIYTFLSENKEEINIQENEEVVIFDENSVDGWVLGENSRGERGLFPKSYVNIIRPRSDSNVTDCSISPAGSLGNDSPHLPSTPNTSINSGQIYDEDYYDDWDDWDDRSTVVDDADHSNTSTTGANGHIFQAPPSNNPNVHYRSKSVSGQDSMASARKGSMVGRNLNRFSSFVRSGVEAFVLGDVPMMAKIAESYTIEMGSLGPQWKANPRPFICSIEDPTKQTKFKGIKTYISYRVTPSHIGRPVYRRYKHFDWLYNRLLHKFTVISVPHLPEKQATGRFEEDFIEKRKRRLILWMNHMTSHPVLSQYEGFEHFLMCADDKQWKLGKRRAEKDEMVGAHFMLTLQIPNEHQDLQDVEERVDNFKTFAKKMDDSVMQLTHVASELVRKHLGGFRKEFQRLGNAFQSISQAFTLDPPYRSDALNNAISHTGRTYENIGEMFAEQPKYDLFQMLDKLSLYQGLLANFPDIIHLQKGAFAKVKESQRMSDEGKMDQDEADGIRKRCRTVGFALQAEMSHFHQQREVDFKDMMQAYLREQIAFYQRVGQQLERTLRMYDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWE9</accession>
    <name>A0A3B5MWE9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWE9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000028016"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028357.1">
        <property type="protein sequence ID" value="ENSXCOP00000028016.1"/>
        <property type="gene ID" value="ENSXCOG00000020898.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234592"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="entry name" value="Extracellular Endonuclease, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044929">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001604">
        <property type="entry name" value="Endo_G_ENPP1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039015">
        <property type="entry name" value="ENDOD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020821">
        <property type="entry name" value="ENPP1-3/EXOG-like_nuc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21472">
        <property type="entry name" value="ENDONUCLEASE DOMAIN-CONTAINING 1 PROTEIN ENDOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21472:SF19">
        <property type="entry name" value="ZGC:172339"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01223">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00892">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00477">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017310599" evidence="1">
        <location>
            <begin position="21"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/RNA non-specific endonuclease/pyrophosphatase/phosphodiesterase" evidence="3">
        <location>
            <begin position="57"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="ENPP1-3/EXOG-like endonuclease/phosphodiesterase" evidence="2">
        <location>
            <begin position="58"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000028016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="296" mass="34019" checksum="A07C9E0D80936CE7" modified="2018-12-05" version="1">GTPRALRKLLYFLIVVMAGAEVQDNISPECKQFLYMGTIPRGLEQQPLKKICQYYAGKPRFFTLYDSFSHIPVYSAYTFKRSDGSKKVDIPWMYEPQLSLVSGSREMEPFPSANIHKSFEDSQAVLDDYSDSVIFERGQLNPDEHQADPEDKAATYTLTNVVPRVREFNIGPWKMHEHTTRKRLNNYCRGTAYVVTGITTSGHTIRRHNINRLGVPTYLWSAYCCIEFDHNAPYSERSKFPAFAAYGLNDRENNKVQEMTIQQLEDFLKRVTYVSSSFQIFYDNCVPPNTANSPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWF3</accession>
    <name>A0A3B5MWF3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RING finger protein 141</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWF3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000028021"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028362.1">
        <property type="protein sequence ID" value="ENSXCOP00000028021.1"/>
        <property type="gene ID" value="ENSXCOG00000020902.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003145"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16545">
        <property type="entry name" value="RING-HC_RNF141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING-HC_RNF141"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF3">
        <property type="entry name" value="RING FINGER PROTEIN 141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="151"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000028021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="177" mass="19522" checksum="00BC300517351081" modified="2018-12-05" version="1">MGQQISGQINHLPEKLVKHAGLVRESGYLTYEEFLGRVAELNDVTAKLAAGQQKHLLFEVQPGSDASALWKVAVRIVCTKINKESGVVEASRIMNLYQFIQLYRDITSQAAEVLSAEGAVRGSSAQLPSTDSCQASMWMGRVKQLTDQEECCICMDGKADLILPCAHSFCQKCIDKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWQ0</accession>
    <name>A0A3B5MWQ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Uridylate-specific endoribonuclease</fullName>
            <ecNumber evidence="11">4.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ribonucleotidyl-uridine-RNA = a 5'-end dephospho-uridine-RNA + a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA</text>
            <dbReference type="Rhea" id="RHEA:67792"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17354"/>
            <dbReference type="Rhea" id="RHEA-COMP:17356"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173117"/>
            <dbReference type="ChEBI" id="CHEBI:173224"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ENDOU family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.-" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWQ0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026178.1">
        <property type="protein sequence ID" value="ENSXCOP00000025867.1"/>
        <property type="gene ID" value="ENSXCOG00000019330.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063825"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21159">
        <property type="entry name" value="XendoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039787">
        <property type="entry name" value="ENDOU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037227">
        <property type="entry name" value="EndoU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018998">
        <property type="entry name" value="EndoU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12439">
        <property type="entry name" value="PLACENTAL PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12439:SF33">
        <property type="entry name" value="URIDYLATE-SPECIFIC ENDORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09412">
        <property type="entry name" value="XendoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142877">
        <property type="entry name" value="EndoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51959">
        <property type="entry name" value="ENDOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="EndoU" evidence="12">
        <location>
            <begin position="7"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="274" mass="31355" checksum="CAAD31874F709B51" modified="2018-12-05" version="1">MASGRQANRELTEVLNKLWQLDTNRLKPGTDYKISLQGKAGHVAQGSNNARDRACAPLFAYVDEGKLKNIATFARFINLLDNYEMSTGVSETVTSEELQENRLFIDAIMETELMKYAHNYLVKKGQSPADRGQFKKQLYDIWFRLYHRGKSDSCGFEHVFVGETKYGNEIMGLHNWVQFYLQEKHGHVDYKGYKARDNKDTPDEDDHPVGSSFIGVSPEFEVALFTIIFLMSTEKMMSVVVKVDEYVLELVVYRHGRAIGTSYPKLLSSNNRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXE6</accession>
    <name>A0A3B5MXE6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cortexin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CTXN2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXE6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024943.1">
        <property type="protein sequence ID" value="ENSXCOP00000024645.1"/>
        <property type="gene ID" value="ENSXCOG00000018416.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154412"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020066">
        <property type="entry name" value="Cortexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16736">
        <property type="entry name" value="CORTEXIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16736:SF2">
        <property type="entry name" value="CORTEXIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11057">
        <property type="entry name" value="Cortexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="81" mass="8936" checksum="5722B33B095DAEF8" modified="2018-12-05" version="1">HLIDQPTTSSLVICGNDIMAHSLTLEQKTAFAFVGMLLVFLGLLIIRCFRILLDPYSSMPSSNWADGIEGLEKGTFEYALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MYL0</accession>
    <name>A0A3B5MYL0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CHD subfamily II SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MYL0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025430"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025737.1">
        <property type="protein sequence ID" value="ENSXCOP00000025430.1"/>
        <property type="gene ID" value="ENSXCOG00000018998.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155088"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016581">
        <property type="term" value="C:NuRD complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009462">
        <property type="entry name" value="CHD_II_SANT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009463">
        <property type="entry name" value="DUF1087"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF22">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06461">
        <property type="entry name" value="CHDII_SANT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06465">
        <property type="entry name" value="DUF1087"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01146">
        <property type="entry name" value="DUF1086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01147">
        <property type="entry name" value="DUF1087"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1087" evidence="4">
        <location>
            <begin position="11"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="CHD subfamily II SANT-like" evidence="3">
        <location>
            <begin position="83"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="67"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="237"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="383" mass="43368" checksum="111177F70C98A17E" modified="2018-12-05" version="1">MNEYLSSFKVAQYVVKDEEEEREIIKQEESVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDADWQDDQSDGQSDYSVASEEGDEDFDERSEGLRNDKDKPLPPLLARVGGNIEVLGFNSRQRKAFLNAVMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGQWSMPWMADLEENKRAAAQPDSPGKTPSTGTPADTQPNTPAPGTSLASENEEASKDGEKDAKKEGEADKNGKEPAKANSEVKNLIRRRWGLKNVQRLESDLSMMPTGPNGDAGEEKRRGADGDGQTEQRRSRESKGKGRRGGRGREKELRGRRGRQTSCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZX0</accession>
    <name>A0A3B5MZX0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MZX0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027837"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028173.1">
        <property type="protein sequence ID" value="ENSXCOP00000027837.1"/>
        <property type="gene ID" value="ENSXCOG00000020758.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002390"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045055">
        <property type="term" value="P:regulated exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31004">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 79"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31004:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 79"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="373" mass="41047" checksum="0493A5340A1BC2DB" modified="2018-12-05" version="1">MDVSLSDDPLLKATLTENGGGTNKKRDADEPAAMLEPETLQWPEDRETGMGGRTDGVIARSDASSWTESERGRRMERAELIGEGRDHLEENRLPEKAAQVFSPAVTVLPSLAASRDTEAFWEMESERSPFLKPRQVDYNQHDLCSRWVGLNPHLSVSPVCPGKGGCSDRDALKLGVSVMTSALLFPFLVWGGFVFLPFDAPLLDGAPLRLVYTLRCSVFAAAPVVFGETERRDNLGLTCLSLTCLTCHPLLPQAELQEVSVHRRFISDSASLFLIYFLQLVVMAMYLTQEQLKLVPLLTIVFAFGQLAYWLAAVFGSSLRGFGFGLSFLPSLVMMAANFYFIFTVESAGSVFGAAPPPDEALPLPTGRQRFWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N1V3</accession>
    <name>A0A3B5N1V3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N1V3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027820"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028155.1">
        <property type="protein sequence ID" value="ENSXCOP00000027820.1"/>
        <property type="gene ID" value="ENSXCOG00000020744.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153801"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008670">
        <property type="term" value="F:2,4-dienoyl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05369">
        <property type="entry name" value="TER_DECR_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43658:SF8">
        <property type="entry name" value="17-BETA-HYDROXYSTEROID DEHYDROGENASE 14-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43658">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="325" mass="34703" checksum="796D293D9BFC2ECA" modified="2018-12-05" version="1">MTKGGGAKAGSENLDAAGGGRTAPPMYHHGNRLQCPLQPPVQSGLARPFGFFAPSEGAMLPAGSFRDRLAFITGGGTGLGRAMTVVLSQLGARCVIASRKLDVLQETASEISSQTRNQVHALQCDVRDPQAVAHCVDQMVALSGLPDVIINNAAGNFICPTERLTPNGWRSVTDIVLNGTAFVTLELGKRLIQAQKGQNQNRDQNQNLSRSNRILVSQAPRSSPSPPSTSLAAEWGRYGHRFNIIQPGPIKTKGAFSRLDPTGTFEKSMIDRIPTGRLGKPAELANLAAYLSSDFASWMSGAVIRFDGGEYVSMAGEFNELRKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KLY5</accession>
    <name>A0A3B5KLY5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">THD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tumor necrosis factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KLY5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001141.1">
        <property type="protein sequence ID" value="ENSXCOP00000001128.1"/>
        <property type="gene ID" value="ENSXCOG00000000934.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177239"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006052">
        <property type="entry name" value="TNF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00229">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00207">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50049">
        <property type="entry name" value="THD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THD" evidence="2">
        <location>
            <begin position="38"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="173" mass="18939" checksum="961134DFA88CFCF0" modified="2018-12-05" version="1">MRKGADQEMEALSGIIVQSEQEVCHSGLDAKLTDANGPSADSVNVANLLSGSQLEWRARFGGGAYSRGLWAIVVPETGLYFVYMKFVLKGPARDFRVTLQRRSKGYDRDMDLMGAQDGGPYPTGPPDGGGGHHRVRTVYVGQLFDLWRGDQLKVLISEGQELIDRASFGAFRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KM67</accession>
    <name>A0A3B5KM67_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KM67"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001208"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001226.1">
        <property type="protein sequence ID" value="ENSXCOP00000001208.1"/>
        <property type="gene ID" value="ENSXCOG00000000991.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154905"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044295">
        <property type="term" value="C:axonal growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045198">
        <property type="term" value="P:establishment of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000013">
        <property type="entry name" value="unconventional myosin-IXa isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046987">
        <property type="entry name" value="Myo9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46184:SF3">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46184">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IXB-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="10">
        <location>
            <begin position="14"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="107"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="201" mass="22628" checksum="B125072357AA6932" modified="2018-12-05" version="1">MVERGFLPQPAGNDPPSDLCALPELTERALLESLRTRFRQEKIYTYVGSILIVINPFQFLPIYNPKYVKLYDNHTLGKLEPHIYAVADVAYHAMLQRRKNQCIVISGESGSGKTQSTNFLIHHLTALSQKGFASGVEQIILGAGPVLEAFGNAKTAHNNNSSRFGKFIQVNYQESGTVRGAYVEKYLLEKSRLVYQEHNER</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KNI4</accession>
    <name>A0A3B5KNI4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="AlphaFoldDB" id="A0A3B5KNI4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000027"/>
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        <property type="protein sequence ID" value="ENSXCOP00000000027.1"/>
        <property type="gene ID" value="ENSXCOG00000000024.1"/>
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    <dbReference type="GeneTree" id="ENSGT01040000244599"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:extracellular matrix binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Kazal_dom"/>
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        <property type="entry name" value="Kazal_dom_sf"/>
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        <property type="entry name" value="BM-40"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR13866">
        <property type="entry name" value="SPARC OSTEONECTIN"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Kazal-like" evidence="4">
        <location>
            <begin position="39"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="100" mass="10736" checksum="F60725A3A2811741" modified="2018-12-05" version="1">SMILSWFIVMSMPADRLRLKKQDKGTPSKAARGLIFLGVPAALGANMWLGCHPSISVTVEQVCGSDGVTYADQCQLRTIACRQDKDVTVQHSGQCTGEPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KP32</accession>
    <name>A0A3B5KP32_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Thrombospondin 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KP32"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001893"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001921.1">
        <property type="protein sequence ID" value="ENSXCOP00000001893.1"/>
        <property type="gene ID" value="ENSXCOG00000001461.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155832"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016525">
        <property type="term" value="P:negative regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Adhesion G protein-coupled receptor B2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thrombospondin 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Thrombospondin 1"/>
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    </dbReference>
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        <property type="entry name" value="Thrombospondin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000041">
        <property type="entry name" value="thrombospondin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1080.10">
        <property type="entry name" value="TSP type-3 repeat"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ConA-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR003367">
        <property type="entry name" value="Thrombospondin_3-like_rpt"/>
    </dbReference>
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        <property type="entry name" value="Thrombospondin_3_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008859">
        <property type="entry name" value="Thrombospondin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
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        <property type="entry name" value="TSP_type-3_rpt"/>
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        <property type="entry name" value="TSPN-like_N"/>
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    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
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        <property type="entry name" value="THROMBOSPONDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10199:SF78">
        <property type="entry name" value="THROMBOSPONDIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02412">
        <property type="entry name" value="TSP_3"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="TSP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VWC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="TSPN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103647">
        <property type="entry name" value="TSP type-3 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="TSP3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51236">
        <property type="entry name" value="TSP_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017288499" evidence="10">
        <location>
            <begin position="18"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="domain" description="VWFC" evidence="11">
        <location>
            <begin position="312"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="repeat" description="TSP type-3" evidence="7">
        <location>
            <begin position="553"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="repeat" description="TSP type-3" evidence="7">
        <location>
            <begin position="589"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="TSP C-terminal" evidence="12">
        <location>
            <begin position="628"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="515"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="279"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="553"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="852" mass="95082" checksum="B58574A5B5F9A93F" modified="2018-12-05" version="1">MKVTGICLLLMLCSCEGARVAESRDDNSVFDLFELIRVPSKNHGVTLAKGDDPYSPAYKILNPDLIPPVPESAFRDLVDSVHAERGFLLLLNFKQFKRTRGSLLTVEKRDGSGAVFEIVSNGKANTLDIVYSTENKQQVVSIEEVDLATGNWKNITLFVQEDRAQLFVGCEEVNTAELDAPIQNILTQGTPAGAQLRVGKGAVNDRFMGVLQNVRFVFGTTLAAILRNKGCQSGNDTMIVTNLNGSSAIRTEYTGHKTKDLQMVCGFSCEDLISMFKELKGLGVVVKELSNELRQLTNENKLIKNRIGIHSGVCIHNGIVRKNKDEWTVDDCTECTCQNSATVCRKISCPLIPCANATVPDGECCPRCGTPSDYAEDGWSPWSEWTHCSVSCGRGIQQRGRSCDRINNICEGTSVQTRDCYLQECDKRFKQDGNWSHWSPWSSCSVTCGSGVITRIRLCNSPTPQLGGRDCVEEGRQTEKCEKSPCPKALGWILWRNWGMCMPCQFSTGLLSPPVDSDDDRVGDKCDSNQDIDEDGHQNNLDNCPYIPNANQADHDKDGKGDACDHDDDNDGIPDDKDNCRLAFNPDQLDSDGDGRGDACKDDFDQDNVPDIYDVCPENFDISETDFRKFQMVPLDPKGTSQIDPNWVVRHQGKELVQTVNCDPGIAVGYHEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQITQTYWSSKPTKAQGYSGLSIKVVNSTTGPGEHLRNALWHTGNTAGQVRTLWHDPKNVGWKDFTAYRWHLIHRPRTGLIRVVMYEGKKIMADSGSIYDKTYAGGRLGLFVFSQEMVYFSDLKYECRDDIFNVQLLPHCCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KR40</accession>
    <name>A0A3B5KR40_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transmembrane protein 121B</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMEM121 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KR40"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002623.1">
        <property type="protein sequence ID" value="ENSXCOP00000002588.1"/>
        <property type="gene ID" value="ENSXCOG00000002074.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154822"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026624">
        <property type="entry name" value="CECR6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032776">
        <property type="entry name" value="CECR6/TMEM121"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47399">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 121B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47399:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 121B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14997">
        <property type="entry name" value="CECR6_TMEM121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
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        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
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            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
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        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="370" mass="41341" checksum="5D7C3C3C2A9AE2C8" modified="2018-12-05" version="1">MISESGGVHQNSDLPPSPSSSSLRSAACQLSSSRDTRSTSSSFQPLFSACIMTSGEFQPSAPLLVHKSRRSLLYKSLCFLLLFFQGGVLDFYLILFTDLYWCSWIATDLVVISGWGVFFLKNARSQRERACGFHQKSSILGCNLGEFTYAYLAWLIYVIACTPKVVLILETSILELIALKVPCGVTGFKITVLLSAPLLFCLINSIVVDLNGPTRHHSQSCFTSTCLDLLDSFTLMEMLLRGELPSVYLKYTVITVYFVALAVPVVWLYELTAAELRCRWLWARFSTGLLVNAPLLVVRCFQVYVYKMPVSVFMFKNIFLLACRLLELLEQCAAVQGLRRRTGTNNPAQFSHCVSENDMCPHGYVNTLAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KS72</accession>
    <name>A0A3B5KS72_9TELE</name>
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        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KS72"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001327"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001350.1">
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    <dbReference type="GeneTree" id="ENSGT01030000234530"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:response to bacterium"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
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        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF127">
        <property type="entry name" value="NITR9"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IGv"/>
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    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
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    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
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        <location>
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            <end position="259"/>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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            <dbReference type="ARBA" id="ARBA00022859"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
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            <dbReference type="Ensembl" id="ENSXCOP00000001327.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="326" mass="36781" checksum="E71CAFB29B533952" modified="2018-12-05" version="1">MAVISFFFLGLIQTIQIPDKIPLTEAELSQTVNLTCKVLGFDNGLFFWYKINHGLMVQTVAGGSFNKVKLEQHFLNSRFESKKVEDTYCLIIKNVSKEDEATYFCQAGAAYKMTFVNGTNLVKSYNVIQTSDVKSVLPGTEVTLHCSLLSEKKKKESTDQCADKHDVFWFRAESKSNPGFIYADKKRCSTQAGSSCEYRLTKTIRNSLDAGTYYCAVVTCGEILFGEGIKVETREELFLYAIVLLGGLLACSVLVNITLMSQRRKPKRHCENRIGRFVDGLNYVALNFSSRKSARRKSKRETSDNCTYSNIKLSMKGSIHQRDNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KU54</accession>
    <name>A0A3B5KU54_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KU54"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002062.1">
        <property type="protein sequence ID" value="ENSXCOP00000002032.1"/>
        <property type="gene ID" value="ENSXCOG00000001638.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:T cell receptor complex"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Immunoglobulins"/>
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        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
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        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002032.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="103" mass="11791" checksum="26E2253C162040F4" modified="2018-12-05" version="1">CKGDSVKQTEGEVVAAAGESLTLRCTYETSDTYPYLFWYKQGENSFPKYMLKRYSTTSENSPEFSEDRFDADLNTEAKEKSVNLKIQDLRLSDSAVYYCALRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KUG0</accession>
    <name>A0A3B5KUG0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KUG0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001251"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001269.1">
        <property type="protein sequence ID" value="ENSXCOP00000001251.1"/>
        <property type="gene ID" value="ENSXCOG00000001026.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177608"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="58" mass="6113" checksum="C2DAEF13DB13AD07" modified="2018-12-05" version="1">LFDPPLLPLPCPVVFSTPQLMLTAGPNMAVPPQQAYGYGYTGAPAYGQPPQPSFGYGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KW01</accession>
    <name>A0A3B5KW01_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Glycoprotein Ib platelet subunit beta</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027889073.1">
        <property type="nucleotide sequence ID" value="XM_028033272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KW01"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002712"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002748.1">
        <property type="protein sequence ID" value="ENSXCOP00000002712.1"/>
        <property type="gene ID" value="ENSXCOG00000002157.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114154298"/>
    <dbReference type="KEGG" id="xco:114154298"/>
    <dbReference type="CTD" id="2812"/>
    <dbReference type="GeneTree" id="ENSGT00530000064244"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052313">
        <property type="entry name" value="GPIb-IX-V_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22650">
        <property type="entry name" value="GLYCOPROTEIN IB BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22650:SF7">
        <property type="entry name" value="PLATELET GLYCOPROTEIN IB BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01462">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017250850" evidence="12">
        <location>
            <begin position="20"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="143"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="LRRNT" evidence="13">
        <location>
            <begin position="19"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="14">
        <location>
            <begin position="83"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="206" mass="22728" checksum="8EA6F57FE6C1B08E" modified="2018-12-05" version="1">MTTYLLLCLLLLGGHRSLACPHPCSCQGRRVNCSGRSLTSGSMPPSFPTGTTELRLHDNLLSTLPNGLLDDLTSLRSVSLHGNPWICDCGILYLRAWLRRQTTDVASYLGANCSSPPSLRGRSVVYLTEEEVLESCHYWYCNLALASLGSLLVFVAVQAALLVALIVFLKRFERLTKEAKRTTEESFTAGDGLRENDYELLKDSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZ09</accession>
    <name>A0A3B5KZ09_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycerol-3-phosphate phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.21</ecNumber>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Aspartate-based ubiquitous Mg(2+)-dependent phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Phosphoglycolate phosphatase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Glycerol-3-phosphate phosphatase hydrolyzing glycerol-3-phosphate into glycerol. Thereby, regulates the cellular levels of glycerol-3-phosphate a metabolic intermediate of glucose, lipid and energy metabolism. Was also shown to have a 2-phosphoglycolate phosphatase activity and a tyrosine-protein phosphatase activity. However, their physiological relevance is unclear. In vitro, also has a phosphatase activity toward ADP, ATP, GDP and GTP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 22">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>sn-glycerol 1-phosphate + H2O = glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:46084"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="EC" id="3.1.3.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>sn-glycerol 3-phosphate + H2O = glycerol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:66372"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="EC" id="3.1.3.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="19">Divalent metal ions. Mg(2+) is the most effective.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.21" evidence="8"/>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027894149.1">
        <property type="nucleotide sequence ID" value="XM_028038348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZ09"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003792"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003832.1">
        <property type="protein sequence ID" value="ENSXCOP00000003792.1"/>
        <property type="gene ID" value="ENSXCOG00000002972.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114157407"/>
    <dbReference type="KEGG" id="xco:114157407"/>
    <dbReference type="CTD" id="283871"/>
    <dbReference type="GeneTree" id="ENSGT00940000160577"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043136">
        <property type="term" value="F:sn-glycerol 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006114">
        <property type="term" value="P:glycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000123">
        <property type="entry name" value="glycerol-3-phosphate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006349">
        <property type="entry name" value="PGP_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01452">
        <property type="entry name" value="PGP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF92">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000915">
        <property type="entry name" value="PGP-type_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="17">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="17">
        <location>
            <position position="29"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="312" mass="33741" checksum="E08652A9E25C953B" modified="2018-12-05" version="1">MSGSRCARLGGPLVRQLLDSVDSVLFDCDGVIWRGDQAIPGAARVISLLKQRGKKVFFVTNNSTKTRKMYADKLSSLGFDAAEDEVFGTAFCCAAYLRSECGLRGKVYLVGSDAMRQELEAVGIQAVGVGPDPVSGKQLDWASVPLDPEVQAVVVGFDEHFSYMKLNRAMQYLTQKDCLFVGTNRDTRLPLEGGKAVPGTGCLLQAVETAAQRQAQTVGKPSRFMFECVARRFGLRPERCLMVGDRLDTDILLGSACGLKTLLTLTGVSTAAEAAAHRDSGDDERRAMVPDYYVESIADLLPALQGEDDPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZI2</accession>
    <name>A0A3B5KZI2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Collagen and calcium binding EGF domains 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
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    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    <dbReference type="STRING" id="32473.ENSXCOP00000003036"/>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="P:lymphangiogenesis"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:vascular endothelial growth factor signaling pathway"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:venous blood vessel morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:venous endothelial cell migration involved in lymph vessel development"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000010">
        <property type="entry name" value="Pro-epidermal growth factor"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="ECM_structural_protein"/>
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        <property type="entry name" value="EGF"/>
    </dbReference>
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        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
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        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
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        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
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        <property type="entry name" value="NOTCH1_EGF-like"/>
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        <property type="entry name" value="COLLAGEN AND CALCIUM-BINDING EGF DOMAIN-CONTAINING PROTEIN 1"/>
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    </dbReference>
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        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
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        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
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        <location>
            <begin position="104"/>
            <end position="145"/>
        </location>
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        <location>
            <begin position="269"/>
            <end position="324"/>
        </location>
    </feature>
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        <location>
            <begin position="309"/>
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    <evidence type="ECO:0000256" key="1">
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        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="324" mass="37154" checksum="3783E65326ABB303" modified="2018-12-05" version="1">MKTSRNVNGTIRLWYDSASIFCLVIFLFVNISSKVNLISYRRKCCKGFRFVMGQCIPEDYDVCAGAPCEQQCTDHFGRVVCTCYLGYRYDRERHRNREKPYCLDIDECADKNMSACSQICINTAGSFRCECEKGYFLEDDGKTCTKGERVHLFEKCDNLMNAGTCSATCEDFLQIKMAVLQLKQKVWDNRREMFVWQKGLMGPVGPTPDLFHIKRGPRGPLVSLYMMLIFCFVFLISQGPPGSFDFLLLLMADIRNDIADLQSKVYSRPMHSPEEDIPLAPDSWRDTADSLDAGSGEDYKEPPSQTGRWSKRNKKRKPGRERTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1K6</accession>
    <name>A0A3B5L1K6_9TELE</name>
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        <submittedName>
            <fullName evidence="4">Required for meiotic nuclear division 1 homolog</fullName>
        </submittedName>
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        <name type="scientific">Xiphophorus couchianus</name>
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        <lineage>
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            <taxon>Atherinomorphae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
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        <property type="nucleotide sequence ID" value="XM_028000759.1"/>
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        <property type="protein sequence ID" value="ENSXCOP00000004220.1"/>
        <property type="gene ID" value="ENSXCOG00000003318.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of mitochondrial translation"/>
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    </dbReference>
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        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF1">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <begin position="206"/>
            <end position="383"/>
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            <begin position="118"/>
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    </feature>
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            <begin position="124"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="429" mass="49421" checksum="7CD7F869F693927B" modified="2018-12-05" version="1">MLLRTLWSRLGARQLAGSRPVCTVSSTTLTQCLKPSNNESTPRIHSWASTNKITFYLNTFNQQCMHKTHVHPAIAGFNNYYSVPLLTLPGTPIHCRCDQARFYSSNIVKSVLKPTLKPVIVPPKGKRPPKGPRTKQPSRSNQPSKKEDEDMMQCIAFATADQYHLPTLSHDLINHGFQEIDLPRDASNVLVISTENAAKVDDDALIFFFREGSVVFWNVEEKEMKKVLRLLEHHEIQPYEVALVHWENEEINYTVGEGNTKLERGNFILSYDMDQQEAVLKKFAFSNALCLSVKLAIWEVALDNFVESIQSIPEMLKSGRRIKLSSAEVMQKIGELFTLRHCINLRSDLLLTPDFYWDRENLEKLYDKTCQFLSINRRVNVVNEKLEHCSQLTDLMRSHLSEKHSLRLEWMIVILITIEVMFELAKMIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1M3</accession>
    <name>A0A3B5L1M3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1M3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001729.1">
        <property type="protein sequence ID" value="ENSXCOP00000001704.1"/>
        <property type="gene ID" value="ENSXCOG00000001376.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="110" mass="12360" checksum="9038A3EFC9059804" modified="2018-12-05" version="1">RQRVILNPLHHREEVGSSGAAGGVRSTSKFPKNFPTMGSGSINKIRGYSKVPNQGNQGCFQWCTAQPQRWSHLFCALITCFLEAGKVLLQKGGIYRRLTLLWFESHNMIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L426</accession>
    <name>A0A3B5L426_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">WD40 repeat domain 95</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L426"/>
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        <property type="gene ID" value="ENSXCOG00000005464.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051242">
        <property type="entry name" value="WD-EF-hand_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324">
        <property type="entry name" value="WD40 REPEAT DOMAIN 95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44324:SF4">
        <property type="entry name" value="WD40 REPEAT DOMAIN 95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="226"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="313"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="453"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="536"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="623" mass="70332" checksum="D8C18A3ADBCD5CE4" modified="2018-12-05" version="1">QYEAKMETVRRRKQVAFHLPAIMNDGGHGVPVVNIHSTHDGGVLTMREDGFICQWSPDLKPQRTKHMFSQGSGKRSSKWITDFTMMSEYNKLMIGTDREIQFYELSTLEPYCQISGLETIPLTLSYLWNRLPKAENVPNIAIKNAVLSKNVTFVRWKVHHDWVTQVKYFHNFQAVVSSSNEDASSLVLQTIFSIYKGVKAFDLCQKHSLLVTGGMDRLIRKPTGILKGHSAPIFSLFISSEDGRIFSISRDNTVKIWHIEDQCCLFTADPTASGIHGDVSACSYSPAMRSLYIAADCIAVLPLMLPKPHSGISVSHDEAVMCCGYSEEFRQVVSCSEEAVVKVWDFDTGRQVFEFTATPDLTSITCMTFDIKGRLITGGSNGYIKVWNFNSGHCIKTLKKGKCHQVCDCTFLKVQGNYVIAVGRDRRIDIYSDSPEELYHVQRPQPSWKDDLQNGHTEDILCVVQSPPSLLATGSYNGEIIVWNTDSGSINQQKITKLYITDEKTLLYAADRMGYIYIYNMETFDPEQRSPREHCWRAHTSSITLQIVTSDQVVLTSSTDHTVRLWSASGEFITFGQPEMWSVQIPSSWIHPGVPYEVLIDPLSMPHHDILKRKSHLSEAISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L476</accession>
    <name>A0A3B5L476_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Melanocortin 2 receptor accessory protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MRAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L476"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007123"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007214.1">
        <property type="protein sequence ID" value="ENSXCOP00000007123.1"/>
        <property type="gene ID" value="ENSXCOG00000005506.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01010000222559"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031780">
        <property type="term" value="F:corticotropin hormone receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030545">
        <property type="term" value="F:signaling receptor regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070996">
        <property type="term" value="F:type 1 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031781">
        <property type="term" value="F:type 3 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031782">
        <property type="term" value="F:type 4 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031783">
        <property type="term" value="F:type 5 melanocortin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106070">
        <property type="term" value="P:regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028111">
        <property type="entry name" value="MRAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28675:SF2">
        <property type="entry name" value="MELANOCORTIN-2 RECEPTOR ACCESSORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28675">
        <property type="entry name" value="MELANOCORTIN-2 RECEPTOR ACCESSORY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15183">
        <property type="entry name" value="MRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="46"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="92"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="137"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="17416" checksum="76232A59402BB6A7" modified="2018-12-05" version="1">MTSYFSKNDTIVPGNSTSAPLYEWEYYLDYLDPVFVDESQLKYHKYSIVIILWVSLAAFVGFLFLILNLMSLIGLLVCLHGERCPDARGRSQESAQTGSTEQPSHPICKEEITQTPASMTKRHSYEVLPEVSADGQSHTEKFTDEWEWKTFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4M4</accession>
    <name>A0A3B5L4M4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SUZ RNA-binding domain-containing</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SZRD1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SZRD1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L4M4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005767"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005832.1">
        <property type="protein sequence ID" value="ENSXCOP00000005767.1"/>
        <property type="gene ID" value="ENSXCOG00000004483.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005532"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024771">
        <property type="entry name" value="SUZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024642">
        <property type="entry name" value="SUZ-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039228">
        <property type="entry name" value="SZRD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31796">
        <property type="entry name" value="SUZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31796:SF2">
        <property type="entry name" value="SUZ DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12752">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12901">
        <property type="entry name" value="SUZ-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51673">
        <property type="entry name" value="SUZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51938">
        <property type="entry name" value="SUZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUZ" evidence="4">
        <location>
            <begin position="39"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="SUZ-C" evidence="5">
        <location>
            <begin position="114"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="49"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="107"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00044802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="160" mass="17816" checksum="94D805AB6266811A" modified="2018-12-05" version="1">MEDEEVAESWEEAADSGIFLLRKKLKWFYFHRKTSLKSASSPVRTAIVVQDDSLPAAPPPQIRILKRPSNNGTAGNSASSSRPSQQMKSLAQREAEYAEARRRILGSASSEETPQDNPSQDRASRMNVQQQQPLEIVRPNNHAIRQPSGPDGTSGFRLCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4V6</accession>
    <name>A0A3B5L4V6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Chemokine interleukin-8-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intercrine beta (chemokine CC) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L4V6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007353"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007448.1">
        <property type="protein sequence ID" value="ENSXCOP00000007353.1"/>
        <property type="gene ID" value="ENSXCOG00000005680.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000288024"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00272">
        <property type="entry name" value="Chemokine_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000002">
        <property type="entry name" value="C-C motif chemokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015:SF183">
        <property type="entry name" value="C-C MOTIF CHEMOKINE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017432017" description="Chemokine interleukin-8-like domain-containing protein" evidence="7">
        <location>
            <begin position="23"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="8">
        <location>
            <begin position="22"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="85" mass="9730" checksum="A4F07E76F70EE195" modified="2018-12-05" version="1">MKGSLFMVAKYLIVFFSGPNVALHCCFTFSTRMVAKEQIRSYSITGSHCSKSAVLFVMMSGSYICVNPKLFWVQKIMKDLDKKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L592</accession>
    <name>A0A3B5L592_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L592"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005575"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005641.1">
        <property type="protein sequence ID" value="ENSXCOP00000005575.1"/>
        <property type="gene ID" value="ENSXCOG00000004348.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234588"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060048">
        <property type="term" value="P:cardiac muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003009">
        <property type="term" value="P:skeletal muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050875">
        <property type="entry name" value="Troponin_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738">
        <property type="entry name" value="TROPONIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738:SF31">
        <property type="entry name" value="TROPONIN I TYPE 2B (SKELETAL, FAST), TANDEM DUPLICATE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="167" mass="18879" checksum="C2B4EF9F403F71E3" modified="2018-12-05" version="1">KSERHFLSHKKHLVLSVAATWLEKEKKDLVVAKETYMAENCPAPNLSGDQATLMETCKKLHAVIDKVDEERYDLEAKVGKADKEIEDLKIKVVDLAGVKKPALKKVRMSADAMLKALLGSKHTVNLDLRANLKQVKKETKEEPTEAVGDWRKNIEDKADRKKMFESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L593</accession>
    <name>A0A3B5L593_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Fatty acid desaturase 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; polyunsaturated fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027884094.1">
        <property type="nucleotide sequence ID" value="XM_028028293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L593"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005006"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005066.1">
        <property type="protein sequence ID" value="ENSXCOP00000005006.1"/>
        <property type="gene ID" value="ENSXCOG00000003925.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114151212"/>
    <dbReference type="KEGG" id="xco:114151212"/>
    <dbReference type="GeneTree" id="ENSGT00950000182990"/>
    <dbReference type="UniPathway" id="UPA00658"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016717">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03506">
        <property type="entry name" value="Delta6-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353:SF12">
        <property type="entry name" value="ACYL-COA 6-DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353">
        <property type="entry name" value="FATTY ACID DESATURASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015921">
        <property type="entry name" value="FA_sphinglp_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="13">
        <location>
            <begin position="19"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="445" mass="52320" checksum="82CDC49F5F1A313A" modified="2018-12-05" version="1">MGGGGQQTEPAEPGSGKTAGVYTWEEVQKHRNRNDQWLVVNRKVYNISQWVKRHPGGSRVIGHYAGEDATEVFAAFHPDQKFVQKFLKPLLIGELVAAEPNQDGNKSAELIDEFDRLREQTEKRGLFQAQPLFFCLHLGHIVLLEALVFLLLSHWGTSWTMTLLCSVLLATAQSQAGWLQHDFGHLSVFKKSKWNHLVHKFVIGHLKGASANWWNHRHFQHHAKPNIFKKDPDINMMDIFVLGNTQPVEYGIKKIKHYPYNYQHQYFFLVAPPLLIPVFYNYNIMKTMFTRRDWVDLAWASTYYIRYFYCFVPLYGVFGSLALMMFVRFLESHWFVWVTQMSHLPKEINHEKRQDWLTMQLQATCNIEQSFFNDWFSGHLNFQIEHHLFPRMPRHNYQLVAPQVRELCEKHGIPYETKTLWQGMVDVVRSLKISGEMWLDAYLHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5D6</accession>
    <name>A0A3B5L5D6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="1">PPIase</shortName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PPIL6</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5D6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005615"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005681.1">
        <property type="protein sequence ID" value="ENSXCOP00000005615.1"/>
        <property type="gene ID" value="ENSXCOG00000004376.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159634"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000048">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="2">
        <location>
            <begin position="126"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="293" mass="33303" checksum="BF853F2282E5D8A7" modified="2018-12-05" version="1">MASQTQIEVVGIIKDPNFHAARSIAEVLKQRFPNDFLDPEIVPLLEFDWHEYLWKKKRELRGDVWEYPSNVICFLNGVLLGNEKQLASWAENQWNFTLTHPEDFYTAITEECYAEHLQKTGHKFVFMDIVVAGEDPRRISFELFSDLCPKTSENFEALCTGERGKSQSGLPLHYKGSLFHRIVPNGWVQGGDISPHSRGNGGESIYGPTFEDESFAISHSKRGTLGMANKGPHTNGSQFYITLQPTPWMDKNYVAFGQVIEGVEVLKKLEESKTFNERPKYECRVSACGVYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5S8</accession>
    <name>A0A3B5L5S8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ELAV-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the RRM elav family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5S8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003119"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003156.1">
        <property type="protein sequence ID" value="ENSXCOP00000003119.1"/>
        <property type="gene ID" value="ENSXCOG00000002464.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156823"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12771">
        <property type="entry name" value="RRM1_HuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000006">
        <property type="entry name" value="ELAV-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000005">
        <property type="entry name" value="ELAV-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000017">
        <property type="entry name" value="ELAV-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006548">
        <property type="entry name" value="ELAD_HU_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002343">
        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01661">
        <property type="entry name" value="ELAV_HUD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 4 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="44"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="130"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="269"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="352" mass="38473" checksum="DDCD33646AF49428" modified="2018-12-05" version="1">LVIATMETQLSNGPTCNNTSNGPSTISNNCSSPVESGSIEDSKTNLIVNYLPQNMTQEELKSLFGSIGEIESCKLVRDKITGQSLGYGFVNYVDPKDAEKAINTLNGLRLQTKTIKVSYARPSSASIRDANLYVSGLPKTMTQKELEQLFSQYGRIITSRILVDQVTGVSRGVGFIRFDRRIEAEEAIKGLNCQKPPGATEPITVKFANNPSQKTSQALLSQLYQSPNRRYPGPLAQQAQRFSRFSPMAIDGVTSLAGINIPGHAGTGWCIFVYNLAPDADESILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYDEAAVAIASLNGYRLGDRVLQVSFKTNKTHKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6T3</accession>
    <name>A0A3B5L6T3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6T3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006150"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006221.1">
        <property type="protein sequence ID" value="ENSXCOP00000006150.1"/>
        <property type="gene ID" value="ENSXCOG00000004752.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164475"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001946">
        <property type="entry name" value="Antigen WC1.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000031">
        <property type="entry name" value="RIKEN cDNA 5830411N06, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF23">
        <property type="entry name" value="SI:CH211-150O23.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="4">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="101"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="21"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="34"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="365" mass="39973" checksum="01076A4C363FBDB2" modified="2018-12-05" version="1">GDHHCSGRVEVNLHEKWGTICDHDWDMQAASVVCSELGCGFAESASSGAEFGPGGGLIALRSVHCKGHESSLVHCDAFINRNSICTHQNDAGVKCSTLMEPRLSVLSPHFVFSAGESVHFYCNIQPRDRVSNVHLYKHGVETPLVTQSADPTQIGVSLVLSDVETFQQGSYSCQYNIKGGFPPHRITSPTSHPVNITVVDLLTPHHWYNTSSEAPTGSVIKGEGFNITCSTPRQYPGASFQLRLVRSNGTVRQSLPAVSQSVTFTFPNAQSSNEGYYYCLYRVQLDGRTFVSRESQPLPIAIKPDPLLSPMVISWLVSGVTFVIAVIIIFTVAKVMCNKEKKPSELERETRTVDNTYVALSVNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L721</accession>
    <name>A0A3B5L721_9TELE</name>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <location>
            <begin position="175"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="256" mass="29509" checksum="3E79038BE1CC7CCF" modified="2018-12-05" version="1">MVVLTNDEEQDYILIEERKCVSLPCSPAKRVSPAKRKQYYINQAVRNSELTPRAKGKKSQRRQENTRYLANLLEKDECSKDDLEVCSNPALPSIFTEACTNGNYIEPWNDFMNCSGEEQERLLSLLEQEGTNRKNANQLLKDHRNVFPAFSAQECFHRIDRRLRTTLKRKQIPMVGVLEVIEEDLLSFFSAEPHSVYTTILSSSFERLLLHAICQYMDLVSASIDYNGSRQTEVMNKQDEFLPPGLLLSAYLEKMS</sequence>
</entry>
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        <scope>IDENTIFICATION</scope>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="76" mass="8267" checksum="AB6D27EB80EB2664" modified="2018-12-05" version="1">MSCDVVSSNQSGVLKHNTLTLGMLYQGGGHGQGRSTTLNFNLYNNTRHEPPAGRWLPLPPPPSAIIVSILMRKLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L831</accession>
    <name>A0A3B5L831_9TELE</name>
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        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
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            <taxon>Atherinomorphae</taxon>
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        </location>
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        </location>
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        </source>
    </evidence>
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        <source>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="155" mass="17368" checksum="8DFA2462BFB9ACE6" modified="2018-12-05" version="1">MEDSETELTVSESDALGADFITVELDTQPIEYVVKWAEVGSKFTISCVKKDSDEAEELTAEPLKIEADEAFFAPYEEVFPCEVTEQSVEIKTDSDEEDEDGEETRLLLEAGGGAPDGEADPDGDYYEPEGRQYRCSYCGKRYSHASSLYRHQQTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9G8</accession>
    <name>A0A3B5L9G8_9TELE</name>
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        <submittedName>
            <fullName evidence="13">Adenosine A2b receptor</fullName>
        </submittedName>
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        <name evidence="13" type="primary">ADORA2B</name>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="entry name" value="Adenosine receptor A2"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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        <property type="entry name" value="Adenosn_rcpt"/>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="entry name" value="ADENOSINE RECEPTOR A2B"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="7 11" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="19"/>
            <end position="283"/>
        </location>
    </feature>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU201114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="331" mass="37380" checksum="3B5B8203B5BA89AC" modified="2018-12-05" version="1">MNASYIAIEAVIAVLSICGNVLVCWAVAINSRLKNATYYFLVSLAVADILVGCLAIPFAIIISVGLYLNFYGCLFLACFVLVLTQSSIFSLLAIAIDRYLAVKIPLRYKELMTGKTARKIIAILWILSFVIGLIPFFGWNLKDSSRENCSSVNSTNCKECYFESVVDMQYMVYFNFFVCVLVPLLIMLGIYVKIFTVARKQLRQIELKCVGNGDNQNKSLLQKEIRAAKSLSIIVGLFTVCWLPVHILNCLTLFYSDLGKPPVVMYVAIILSHANSVINPIIYAYRIQDFRNTFRKILVQHFLCHKEELYLSSNGSRRNRDQVTVTIDPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9X8</accession>
    <name>A0A3B5L9X8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9X8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007642"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007738.1">
        <property type="protein sequence ID" value="ENSXCOP00000007642.1"/>
        <property type="gene ID" value="ENSXCOG00000005880.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157769"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071598">
        <property type="term" value="C:neuronal ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:ribonucleoprotein complex binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046825">
        <property type="term" value="P:regulation of protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12465">
        <property type="entry name" value="RRM_UHMK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000322">
        <property type="entry name" value="Serine/threonine-protein kinase Kist isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000241">
        <property type="entry name" value="Serine/threonine-protein kinase Kist isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034372">
        <property type="entry name" value="UHMK1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034371">
        <property type="entry name" value="UHMK1_RRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46962">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE KIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46962:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE KIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="241"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="339" mass="38042" checksum="ADAEF9864EF1D841" modified="2018-12-05" version="1">MHDVSSCFSVTLYGVFTNHNCVGVATRCLLLELLDVSVSELLVSRPQKGHSMWLVQHCARDILEALAFLHREGYVHADLKPRNVLWSADDECFKLIDFGLSFKQGNQDVKYIQTDGYRAPEAELQNSLAQAGMEGDSGCTAAIDLWSLGVILLEMFSGIKLKDTVRSQDWKDNSVAVVNRIFASNGVMCPAIPVYHLRDLIKSMLLNNPKQRCTAEAALLSPFFSIPFAPHIEDLVLLPSPVLRLLNLIDDSHLHNDEEYEDILEDMKEECQKYGSVVSLLIPKENPGKGQVFVEYANSSDSKEAQRLLTGRTFDGRFVVATFYPLSAYKRGYLYQTVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LA79</accession>
    <name>A0A3B5LA79_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LA79"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006727.1">
        <property type="protein sequence ID" value="ENSXCOP00000006646.1"/>
        <property type="gene ID" value="ENSXCOG00000005145.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287299"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="111" mass="12748" checksum="B9E4A3A0BFE96FAD" modified="2018-12-05" version="1">LWKEKKTYRVILASRYGTCGLLQPLPRRLRPRSERARITLPRASRPVLMILLARSEPARSTRQSLDERTVFKDGVRITLERLEAFPISTFLVTSPLKVCCRFGLPGRPTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAD7</accession>
    <name>A0A3B5LAD7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">FMR1 autosomal homolog 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoplasmic ribonucleoprotein granule</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FMR1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAD7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007802"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007900.1">
        <property type="protein sequence ID" value="ENSXCOP00000007802.1"/>
        <property type="gene ID" value="ENSXCOG00000005798.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183189"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045182">
        <property type="term" value="F:translation regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048170">
        <property type="term" value="P:positive regulation of long-term neuronal synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043488">
        <property type="term" value="P:regulation of mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099577">
        <property type="term" value="P:regulation of translation at presynapse, modulating synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20473">
        <property type="entry name" value="Tudor_Agenet_FXR2_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20476">
        <property type="entry name" value="Tudor_Agenet_FXR2_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000001">
        <property type="entry name" value="Fragile X mental retardation 1, isoform CRA_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000002">
        <property type="entry name" value="Fragile X mental retardation 1, isoform CRA_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000004">
        <property type="entry name" value="Fragile X mental retardation 1, isoform CRA_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000017">
        <property type="entry name" value="Fragile X mental retardation syndrome-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008395">
        <property type="entry name" value="Agenet-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040148">
        <property type="entry name" value="FMR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022034">
        <property type="entry name" value="FMR1-like_C_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040472">
        <property type="entry name" value="FMRP_KH0"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032172">
        <property type="entry name" value="FXR1_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047421">
        <property type="entry name" value="Tudor_Agenet_FXR2_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047420">
        <property type="entry name" value="Tudor_Agenet_FXR2_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041560">
        <property type="entry name" value="Tudor_FRM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10603">
        <property type="entry name" value="FRAGILE X MENTAL RETARDATION SYNDROME-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10603:SF3">
        <property type="entry name" value="RNA-BINDING PROTEIN FXR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05641">
        <property type="entry name" value="Agenet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12235">
        <property type="entry name" value="FXMRP1_C_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16096">
        <property type="entry name" value="FXR_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17904">
        <property type="entry name" value="KH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18336">
        <property type="entry name" value="Tudor_FRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51641">
        <property type="entry name" value="AGENET_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Agenet-like" evidence="8">
        <location>
            <begin position="4"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Agenet-like" evidence="8">
        <location>
            <begin position="63"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="400"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="440"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="463"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="486"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="523"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="580"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="619"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="640"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="654" mass="72985" checksum="B8B492CDD19BAE55" modified="2018-12-05" version="1">MDGLAVEVRGSNGAFYKGFIKDVHEDSLTIVFENNWQPERQLPFSDVRLPPPADYHKDIGEGEEVEVYSRANEQEPCGWWLARVRMMKGEFYVIEYAACDATYNEIVTSERIRPLNPNSPASRNTFHKMTIPVPEDLRDICASDDIHKDFRKAIGANCIFYSAQTHDLIVLSTNDTTVKRATLLSDMHFRSIRTKLILMSRNEEATKHLETSKQLAAAYQEEVRVREDLMGLAIGSHGANIQQARKVPGVTAIELEEESCTFRIYGETPEAVKQAREYLEFKEDYFQVPRNLVGKVIGKNGKVIQEIVDKSGVVRVRIEGDNDKKLPREEVGRQGAGRDDTASSKEGMVPFVFVGTKENISNAQALLEYHVSYLQEVEQLRLERLQIDEQLRQIGVGYRVAPSRPAGPGGDREKGYMTDESSNSLHASRTYGGRGRGRRPSNTQYSGYGTNSELSNASESEDVNDRDLRPRGIGGDERGSRRGGRGRGANSGRGRGGPAAKPSNSISSVLRDPDSNPYALLEGEGELADADLSEGTVGDRHRRSRRRRNDQEPSVMDAATESDNQAGPSENGLDEEARPQRRNRSRRRRNRGSRPEGGSASRDRQPGESVTVADYISRPESQSRQNLPQKENHSGPEPKVNTQTHQSDSPSQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAF1</accession>
    <name>A0A3B5LAF1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Transmembrane p24 trafficking protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TMED7</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">cis-Golgi network membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAF1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006716"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006798.1">
        <property type="protein sequence ID" value="ENSXCOP00000006716.1"/>
        <property type="gene ID" value="ENSXCOG00000005195.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158463"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017454617" evidence="12">
        <location>
            <begin position="24"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="175"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="13">
        <location>
            <begin position="35"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="213" mass="24402" checksum="3B93B3245D664839" modified="2018-12-05" version="1">MFRSLRLLLQLLWAQLLCGWVMGSELTFELPDNAKQCFYEDIITGTKCTLEFQVVTGGHYDVDCRLEDPDGTTLYKEMKKQYDSFTFTAAKNGTFKFCFSNEFSTFTHKTVYFDFQVGDDPPLFPNENRVTALTQMESACVSIHEALKSVIDYQTHFRLREAQGRSRAEDLNTRVAFWSIGEAIILLVVSISQVVLLRSFFSDKKTTMTRVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAS2</accession>
    <name>A0A3B5LAS2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerase III subunit RPC5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027885894.1">
        <property type="nucleotide sequence ID" value="XM_028030093.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027885895.1">
        <property type="nucleotide sequence ID" value="XM_028030094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAS2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007595"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007691.1">
        <property type="protein sequence ID" value="ENSXCOP00000007595.1"/>
        <property type="gene ID" value="ENSXCOG00000005850.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114152266"/>
    <dbReference type="KEGG" id="xco:114152266"/>
    <dbReference type="CTD" id="55718"/>
    <dbReference type="GeneTree" id="ENSGT00390000016123"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006886">
        <property type="entry name" value="RNA_pol_III_Rpc5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045576">
        <property type="entry name" value="RPC5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12069:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12069">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES III 80 KDA POLYPEPTIDE RNA POLYMERASE III SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04801">
        <property type="entry name" value="RPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19725">
        <property type="entry name" value="RPC5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase III subunit RPC5 C-terminal" evidence="2">
        <location>
            <begin position="444"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="467"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="686" mass="77607" checksum="ED8A0384826CED10" modified="2018-12-05" version="1">MASGDEDDPIIEEIDVYLSKSLADKLYLLQYPVRPGTMTYDDVSHLSAKIKPKQQRLELEMAINTMSPNYCRSKGEQIALNVDGTSYDETNTYRAKMMDKQTFSSMQATTNTSRYAAAVFRKGELHITPLTGILQMRPGFSYLDKADSKTREREAANEGGDSSQDEAEEEAKAITVRFARPESEQARQRRIQSYEFLQKKQAEEPWIHLQYHSAKDSRSDHEKQYLYCQSVDASQNSELVKTPKEYLAMLMPPLAEEKIAKPVGPSNVLSMAQLRTLPLGEQVKTLMKNVKVMPFANLMGLLASGTDQTSVLRCIQQVALLVQGNWVVKSDVLYPKNTCSAHSGVPAEVLCRGRDFVMWRFTKERSVMRKEMTSIIKLPPEDVKEFLEHVASPRVNRGWEFLLPTDADFIKKHPDVAHRQNMLWLGIQSKLEKVFNFSKEDFMPKNSPQPEPVHVSGAQRLKFAQERAQDNQASLQRDLDGRRAGSSVQIKQEPNGDVAEELMDTSSSIHNGSVNGYSSATSPCLDHTNGGSPTNSLTPELQDFVTKTFQKHIVLTLNELKRLFNLYLASIPAGQNVFSSISDHMLQDAVLLCHCKQIMVPFPAQSKLAPDERKVFGLWETGDNFDKHRRLLYDLFTKNHRVRRNVIQARLAQDLGYVSKAEVDRLLHECCISSAGFWYLKGTLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAU9</accession>
    <name>A0A3B5LAU9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAU9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009439.1">
        <property type="protein sequence ID" value="ENSXCOP00000009328.1"/>
        <property type="gene ID" value="ENSXCOG00000007103.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155053"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="102"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="218" mass="25579" checksum="8BB10E72F88D679A" modified="2018-12-05" version="1">TIMTSEGELLPWRRDNKEKDRKCQRRKETKWRRKETKWRRTVTPSRNPVVQDIPESVEDFLRNFLRKLGLTRTLSLFELEWHSRARGGGGGGIFVPDALTHRQALLEKLEAVRGDAEQLRKQVLTAAQSLLRMQQESDYHRQQHRRLSEQKHVLTGDCKQLQEQLQDSEEALKRLRDKHEAALRTKTLLGLQKERAQRPGLTRTLEQMYGGPILEKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LB12</accession>
    <name>A0A3B5LB12_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LB12"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005059"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005120.1">
        <property type="protein sequence ID" value="ENSXCOP00000005059.1"/>
        <property type="gene ID" value="ENSXCOG00000003971.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154078"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045329">
        <property type="entry name" value="LZTS"/>
    </dbReference>
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        <property type="entry name" value="ZIPPER PUTATIVE TUMOR SUPPRESSOR 2 HOMOLOG-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZIPPER PUTATIVE TUMOR SUPPRESSOR 2-RELATED"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="24"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="107"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="227"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="125"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="285" mass="31282" checksum="8026AB7CF963D079" modified="2018-12-05" version="1">MEKTVLRPTAFKPVIPKSRSAMQYLSPRHGASAPEGQNNLNLLSPAHREVSPSCSEKRSSYSGGRNASSGSSQSCSLSDSGRNSLSSLPPFNGATYSLASAEVPAGHLEPIRSVPSVGTHGHTNSDSGRSSSSKSTGSGSVSGRGQHQSDSGSGGRSPGPVEGYEGVVRDLEDKLRERDLELQQLKDNLDDNEAAICQKRFELELEELRQSCATRMQVASQKAQRAQQVLQLQVYQLQQEKKKLQEDFAQLLKEREQLEERCTSYEHEKIQLGPRLEETKWEVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBN6</accession>
    <name>A0A3B5LBN6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Granulins domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the granulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBN6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005284"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005345.1">
        <property type="protein sequence ID" value="ENSXCOP00000005284.1"/>
        <property type="gene ID" value="ENSXCOG00000004138.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.160">
        <property type="entry name" value="Granulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000118">
        <property type="entry name" value="Granulin"/>
    </dbReference>
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        <property type="entry name" value="Granulin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037277">
        <property type="entry name" value="Granulin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12274">
        <property type="entry name" value="GRANULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12274:SF6">
        <property type="entry name" value="GRANULIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00396">
        <property type="entry name" value="Granulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00277">
        <property type="entry name" value="GRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57277">
        <property type="entry name" value="Granulin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00799">
        <property type="entry name" value="GRANULINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Granulins" evidence="5">
        <location>
            <begin position="36"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="82" mass="9004" checksum="78AE674BD47639D6" modified="2018-12-05" version="1">MCDGQTSCPKDTTCCFMERTHKWGCCPLPEVGAVCCKDGIHCCPSGHTCDPHRTAVLRATHATCKLKHVTRKATIWSSPLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LD28</accession>
    <name>A0A3B5LD28_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Gamma-aminobutyric acid type A receptor subunit alpha6b</fullName>
        </submittedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LD28"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007601"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007697.1">
        <property type="protein sequence ID" value="ENSXCOP00000007601.1"/>
        <property type="gene ID" value="ENSXCOG00000005851.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156722"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19052">
        <property type="entry name" value="LGIC_TM_GABAAR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000001">
        <property type="entry name" value="Gamma-aminobutyric acid A receptor subunit alpha-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000067">
        <property type="entry name" value="Glycine receptor subunit alpha-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001390">
        <property type="entry name" value="GABAAa_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047024">
        <property type="entry name" value="Gabra-1-6_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01079">
        <property type="entry name" value="GABAARALPHA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5022264295" evidence="18">
        <location>
            <begin position="28"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="316"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="428"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="44"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="258"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="455" mass="51565" checksum="F5AE59EAB7D84EFA" modified="2018-12-05" version="1">MNHTFLNFNVFFLVFFFFASLSCKGHAYSIKAEREQYIYLDNITQILDRLLDGYDNRLRPGFGGPVTEVKTDIFVTSFGPVSDVEMEYTMDVFFRQTWIDERLKFEGPIEILRLNNLMVSKIWTPDTFFRNGKRSISHNMTTPNKLFRIMQNGTILYTMRLTINAECPMRLMNFPMDGHACPLKFGSYAYPISEIVYTWKKGPLSSVEVPQESSSLLQYDLIGQTVSSERLKSNTGEYVIMTVHFHLQRKMGFFLIQTYIPCIMTVILAQVSFWINKESVPARTVFGITTVLTMTTLSISARHSLPKVSYATAMDWFIAVCFAFVFSALIEFAAVNYFSTLQANRELRKAAAIKAAAQEATAAAAVVPAVDANSVLKKRMNSAPVFDRPAKTFPNPPVNAQAFLQQGSAVPANNVLTGTSIIDKYSRILFPLSFGAFNLVYWIVYLTKDTMEMSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LD61</accession>
    <name>A0A3B5LD61_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Arginine vasopressin</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the vasopressin/oxytocin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LD61"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008782"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008888.1">
        <property type="protein sequence ID" value="ENSXCOP00000008782.1"/>
        <property type="gene ID" value="ENSXCOG00000006704.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004511"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005185">
        <property type="term" value="F:neurohypophyseal hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.9.10:FF:000001">
        <property type="entry name" value="oxytocin-neurophysin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.9.10">
        <property type="entry name" value="Neurohypophysial hormone domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000981">
        <property type="entry name" value="Neurhyp_horm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036387">
        <property type="entry name" value="Neurhyp_horm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022423">
        <property type="entry name" value="Neurohypophysial_hormone_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11681">
        <property type="entry name" value="NEUROPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11681:SF13">
        <property type="entry name" value="VASOPRESSIN-NEUROPHYSIN 2-COPEPTIN PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00220">
        <property type="entry name" value="Hormone_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00184">
        <property type="entry name" value="Hormone_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001815">
        <property type="entry name" value="Nonapeptide_hormone_precursor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00831">
        <property type="entry name" value="NEUROPHYSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00003">
        <property type="entry name" value="NH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49606">
        <property type="entry name" value="Neurophysin II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00264">
        <property type="entry name" value="NEUROHYPOPHYS_HORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0027">Amidation</keyword>
    <keyword evidence="2" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0372">Hormone</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017180136" evidence="8">
        <location>
            <begin position="20"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="20"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="41"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="59"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="106"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001815-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="153" mass="15644" checksum="9B13CC0B90B350CA" modified="2018-12-05" version="1">GSMHHSLLCVLGLLALSSACYIQNCPRGGKRALPEGGVRQCMPCGPGDRGRCFGPSICCGEGLGCLLGSAAAAHCEEENYLLTPCQPGGRPCGPEGGHCASSGLCCNSEGCMVDSDCLGETDAADPAQSSARSSPTDLLLRLLHVASRGQNDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDB5</accession>
    <name>A0A3B5LDB5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDB5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008832"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008938.1">
        <property type="protein sequence ID" value="ENSXCOP00000008832.1"/>
        <property type="gene ID" value="ENSXCOG00000006734.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161555"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14885">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 43-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14885:SF3">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="196"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="382"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="493" mass="55370" checksum="588653E4E7CAC86D" modified="2018-12-05" version="1">EDGQLMTFGADGAVRSWDFEKINMATMSDSSSSRFEVEPINELLVGYNVCLTSVVRTCVPDSFMWFAQDSNGTIWKLDLSFTNTAADPECLFFFHAGVIQGMDVSKKSHLMATTTLDRSVRVFDFVANKEVAISRFNQGGTMVCWAPPLVNQSGGLLVTGFEDGVVRMLELFNPQRLPTPSRGPVMDAELRLNQAFKPHNAPVTAVAYDRNGEILATGSLDCTVFFFTVSEKYSPIGFILVPGPVKALEWSPHFHSENRLLILCQSGHAVEVPCPDPEDHESTKTFQLQNLPRSSFRFKSIKSKIKRKEREEKIKELKKAGITVPEEEEEEEEEEEELPPIFIPKPPSPLCCGFYSQPGQFWLSMLFVSQPTSTFTPPSIETAKQKTEKDSLRREAEQKEAEMLKHLLELQKTFKEIQSDNESLPEHILLKPQVLEPAAEFSCSTTLQHKEGSTETASGQDIAELNKCTQKCHNYLRNIKISPLMTNLDFILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDP5</accession>
    <name>A0A3B5LDP5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Notochord homeobox</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDP5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005989"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006057.1">
        <property type="protein sequence ID" value="ENSXCOP00000005989.1"/>
        <property type="gene ID" value="ENSXCOG00000004648.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154361"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014028">
        <property type="term" value="P:notochord formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000450">
        <property type="entry name" value="Homeobox protein notochord"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050877">
        <property type="entry name" value="EMX-VAX-Noto_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24339:SF67">
        <property type="entry name" value="GNOT1 HOMEODOMAIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24339">
        <property type="entry name" value="HOMEOBOX PROTEIN EMX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="128"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="130"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="201"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="239" mass="26626" checksum="2BB511BA7E8DAD08" modified="2018-12-05" version="1">MQIPNRPMEAGGYSVRNYARASLCTPSGKSFTIEALLAKPEEAAPRDRSGPNQWGEKYPQTAPPPHLAGLTLPPAQYLYSPNALHPSVHPQPGYSVYCYPPFTYAPSCRGAFYSQANAAMHQFKTKGGKSKRMRTSFTSEQLSRLEKEFARQQYMVGSERFLLASALQLTEAQVKVWFQNRRIKWRKQSLEQQQAKLAKLGLATPLKSPGSQGHGDEGDEDFSDSDVDIDVSDDLVDHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LF45</accession>
    <name>A0A3B5LF45_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">E3 ubiquitin-protein ligase RNF14</fullName>
            <ecNumber evidence="5">2.3.2.31</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">RING finger protein 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">RNF14</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with GCN1; interaction takes place in response to ribosome collisions and is required for ubiquitination of EEF1A1/eEF1A. Interacts with the ubiquitin-conjugating enzymes UBE2E1 and UBE2E2. Interacts with AR/androgen receptor. Interacts with TCF7/TCF1, TCF7L1/TCF3 and TCF7L2/TCF4; promoting Wnt signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the RBR family. RNF14 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LF45"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008311"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008412.1">
        <property type="protein sequence ID" value="ENSXCOP00000008311.1"/>
        <property type="gene ID" value="ENSXCOG00000006366.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154507"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060828">
        <property type="term" value="P:regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20341">
        <property type="entry name" value="BRcat_RBR_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20354">
        <property type="entry name" value="Rcat_RBR_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16628">
        <property type="entry name" value="RING-HC_RBR_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23820">
        <property type="entry name" value="RWD_RNF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000011">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000007">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000049">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000186">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047548">
        <property type="entry name" value="Rcat_RBR_RNF14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031128">
        <property type="entry name" value="RNF14_RING-HC_Zfn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0804">Transcription</keyword>
    <keyword evidence="15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RWD" evidence="24">
        <location>
            <begin position="10"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="25">
        <location>
            <begin position="196"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="23">
        <location>
            <begin position="200"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00062346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00067098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00075528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="454" mass="51514" checksum="CC348704C005E2F9" modified="2018-12-05" version="1">MSEDKEAQENELLALASIYDEEEFRRAESAQGGEIQLCLELPPNFKLLVKGDKTAEYNVCFLPPLVLNFELPADYPSTSPPVFSLSSKWMTKAQLSSLCQRLDELWDENQGFEVLFMWIQFLKEETLNFLSIQSPLELYRSSIKGGGERRKTDPEAPSLGQMSDPRAVVLMDPHSDPLPQLLDFDEAQRQRAFDGKVFCCGICFSDKLGASCLCFKGCQHVYCKVCMTEYFQIQIRDGNVQCLNCPEPECTSLATPSQVKQLVDEELFARYDRLLLQSSLDLMADVVYCPRHSCGTAVMVEPDTTMGICSACQYAFCTLCKLGYHGVSHCKITADDLRSLRDEYLSATPEGQKFMEQRFGKRVVQKAVEESFSRDWLKENCKCCPRCGTNIQKVDGCNKMTCTSCKQYFCWLCLGALSKVNPYSHFNNPHSPCFNQLFHGVDVDDDEFWSDEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LH14</accession>
    <name>A0A3B5LH14_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LH14"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007159"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007251.1">
        <property type="protein sequence ID" value="ENSXCOP00000007159.1"/>
        <property type="gene ID" value="ENSXCOG00000005336.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169195"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000046">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051370">
        <property type="entry name" value="PPIase_Pin1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10657">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10657:SF4">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="WW" evidence="5">
        <location>
            <begin position="5"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="132" mass="15642" checksum="5A86D6E2C2EFB164" modified="2018-12-05" version="1">MGDEENLPSGWEKRMSRSSGKVYYFNHITNASQWERPVGDGRGEPEKVRCSHLLVKHNQSRRPSSWREQNITRSKDEALDLIQSKRLRCHCYMSVIDFKSKDCLLSFLTYSTDQKFGHTFLIQWVFFIFMTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKX2</accession>
    <name>A0A3B5LKX2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Suppressor of cytokine signaling 5b</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKX2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008524"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008629.1">
        <property type="protein sequence ID" value="ENSXCOP00000008524.1"/>
        <property type="gene ID" value="ENSXCOG00000006527.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164285"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046935">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022252">
        <property type="entry name" value="SOCS4/SOCS5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF15">
        <property type="entry name" value="SUPPRESSOR OF CYTOKINE SIGNALING 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12610">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="3" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SH2" evidence="8">
        <location>
            <begin position="389"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="9">
        <location>
            <begin position="454"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="92"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="505" mass="56278" checksum="57B9AF2A0445B5A7" modified="2025-10-08" version="2">MTTVGNMWSSLKSRCQTLFHSTGSAESRVEVETVQCVVDLGETDNAVETRGPAAATASPSWRLMPVPVVATGRRHHNCVSDIPQIVEISIDKESEDSRGTSGGVPMARRDSYSRHAPWGGKKKHSCSTKTQSSLEADRRSGRLRGCGNRRDRRYGISSIQEINDSGSGGRSLNTRSLRQRLSDTVGLCLPLPARQRSHPKNPINSKRKIHLTELMLETCPFPPGSDLAHKWHLIKQHTAPVSPHSSTALLDAFDPAHPSPEDEEERLRERRRLSIEEGVDPPPNAQIHTLEASVPGPSLYKLGPKMAPTGAASLGACGSILGATAASQDCDSEEDSTTLCLQARRPKQRHASGDGHQSRQQPGPWKVHTQIDYIHCLVPDLLQLTALPCYWGVMDRYEAEALLDGRPEGTFLLRDSAQEDYLFSVSFRRYNRSLHARIEQWNHNFSFDAHDPCHLARAAICRWTTYDGIGSLPLPPALQDFLKEYHYKQKVRVRWLEREPPLKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMC6</accession>
    <name>A0A3B5LMC6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alpha-2A adrenergic receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Alpha-2A adrenoreceptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMC6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012117"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012255.1">
        <property type="protein sequence ID" value="ENSXCOP00000012117.1"/>
        <property type="gene ID" value="ENSXCOG00000009161.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161451"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004938">
        <property type="term" value="F:alpha2-adrenergic receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051379">
        <property type="term" value="F:epinephrine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002233">
        <property type="entry name" value="ADR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF24">
        <property type="entry name" value="ALPHA-2A ADRENERGIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01103">
        <property type="entry name" value="ADRENERGICR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 15" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 15" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 15 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="29"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="202"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="225"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="387" mass="44129" checksum="AA793928D0D1EB9F" modified="2018-12-05" version="1">MIIGQPFSVQTSVLLTTLVGMLILLTVFGNVMVVIAVITSRVLKAPQNLFLVSLACADILVSTLVMPFSLANELMGYWYFGTVWCEIHLALDVLFCTSSIVHLCAISLDRYWSVTKAIEYNLKRTPRRIKCTICLVWALAALISFPPLITMTKKEGGKDNPACKINEVKWYIIFSSIASFFAPCVIMIMVYVRIYQVAKKRTRASPGERQRENGNLENTQFDMDPGEKQEKEMDEPKDGEEVYRLDVEEEPSSSDGNENILCSLMKKRSGRTPKVTQVKSKETYTKAEDLSSARASKWKGRQYRERRFTFVLAVVVGVFVLCWFPFFFTYTLAAVCDMCCIPETLFKTFFWFGYCNSSLNPVIYTIFNKDFRKSFKKILCKRNTGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMD1</accession>
    <name>A0A3B5LMD1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000012122"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012260.1">
        <property type="protein sequence ID" value="ENSXCOP00000012122.1"/>
        <property type="gene ID" value="ENSXCOG00000009158.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156061"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003013">
        <property type="term" value="P:circulatory system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009913">
        <property type="term" value="P:epidermal cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090136">
        <property type="term" value="P:epithelial cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060218">
        <property type="term" value="P:hematopoietic stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036303">
        <property type="term" value="P:lymph vessel morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051241">
        <property type="term" value="P:negative regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045597">
        <property type="term" value="P:positive regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010572">
        <property type="term" value="P:positive regulation of platelet activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901222">
        <property type="term" value="P:regulation of non-canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="33"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="CDD" id="cd01450">
        <property type="entry name" value="vWFA_subfamily_ECM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.10">
        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003410">
        <property type="entry name" value="HYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051277">
        <property type="entry name" value="SEZ6_CSMD_C4BPB_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011641">
        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45656:SF4">
        <property type="entry name" value="PROTEIN CBR-CLEC-78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07699">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02494">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="32"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00895">
        <property type="entry name" value="PENTAXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="35"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00159">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="34"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50825">
        <property type="entry name" value="HYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51828">
        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0768">Sushi</keyword>
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        <location>
            <begin position="31"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="12">
        <location>
            <begin position="378"/>
            <end position="439"/>
        </location>
    </feature>
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        <location>
            <begin position="473"/>
            <end position="562"/>
        </location>
    </feature>
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        <location>
            <begin position="562"/>
            <end position="625"/>
        </location>
    </feature>
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            <begin position="1031"/>
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            <begin position="1068"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
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            <begin position="1105"/>
            <end position="1140"/>
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            <begin position="1142"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="1180"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="9">
        <location>
            <begin position="1217"/>
            <end position="1253"/>
        </location>
    </feature>
    <feature type="domain" description="Pentraxin (PTX)" evidence="13">
        <location>
            <begin position="1257"/>
            <end position="1460"/>
        </location>
    </feature>
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        <location>
            <begin position="1516"/>
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            <begin position="1572"/>
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            <begin position="1613"/>
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            <begin position="1727"/>
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        </location>
    </feature>
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        </location>
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        </location>
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            <begin position="2908"/>
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            <begin position="3013"/>
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            <begin position="3072"/>
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            <begin position="3129"/>
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            <begin position="3244"/>
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            <begin position="3361"/>
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        </location>
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            <begin position="728"/>
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        </location>
    </feature>
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        <location>
            <begin position="728"/>
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        </location>
    </feature>
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        <location>
            <begin position="741"/>
            <end position="750"/>
        </location>
    </feature>
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        <location>
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            <begin position="1243"/>
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    </feature>
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            <begin position="1544"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
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    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="1905"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="1994"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="2163"/>
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    </feature>
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        <location>
            <begin position="2223"/>
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    </feature>
    <feature type="disulfide bond" evidence="7">
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    </feature>
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        <location>
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    </feature>
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        <location>
            <begin position="2472"/>
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    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="2530"/>
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    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="2588"/>
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    <feature type="disulfide bond" evidence="7">
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            <begin position="2704"/>
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    <feature type="disulfide bond" evidence="7">
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            <begin position="2762"/>
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    <feature type="disulfide bond" evidence="7">
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            <begin position="2819"/>
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    </feature>
    <feature type="disulfide bond" evidence="7">
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    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="3041"/>
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    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="3099"/>
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    </feature>
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            <begin position="3332"/>
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    <feature type="disulfide bond" evidence="6">
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            <begin position="3350"/>
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    <feature type="disulfide bond" evidence="6">
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            <begin position="3363"/>
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        </location>
    </feature>
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        <location>
            <begin position="3381"/>
            <end position="3390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
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            <dbReference type="PROSITE" id="PS50234"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50825"/>
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    </evidence>
    <evidence type="ECO:0000259" key="12">
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            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="3391" mass="367595" checksum="FDE5D24A1823BF4D" modified="2025-10-08" version="2">MSESAESKVERLGQAFKQNVRELREKSACLDLVFLVDESSSVGSNNFQSELRFIRKMLSDFPVAPENTRVALVTFSSKTHVVTRVDHISAPKSHQHKCSLFNQEIPSINYRGGGTYTKGAFQRAAQILRNSRGNATKAIFLITDGYSNGGDPRPVAAALRERGVEIFTLGIWQGNIRELHDMASQPKDQHCYLVHNFAEFEALARRALHELPTGSYIQEDLSRCASLCDEGKDCCDVMASCKCGMHTGQYDCICEKGYYGKGLKHECPPGTYKPEGTPGGLTTCLSCPDAQHTSQPGSTALHDCVCKPGYQPIGMTCQIHCPALTPPEYGFFVQDVCNNHFEAACGVRCQPGFELHGTGIRLCQADSTWSGTPASCTRSCPVLSRPQHGFLRCSDGGVSYGMQCQVGCERGYRLEGDSTLACQANSQWSGSLPRVRCHPIVNPKNNFLLSPPPCGKRAVSPGFVCHLTCRQGYSLQGNRRAACLGSGNWTANVHKATTEPPFIDRCRSPPTVQATNTEAAVDWEVPQFSDNSFPVGETVVQYTATDTAGNSRVCNLTITVQSTCEQPYVPVNGEFICSEEEEGVNCTLQCKEGYSFAQDAVQSYFCAYNGVWEPAYSSDRPDCSNRIANNGYKPFEMLFKASRCDDADLVKSFAGHFNAKLGSMLPSICSSDEITCKLEVMSQGHCLEYNYDYENGFSIAPGGWANNWGTQGAQDYAYFESGFATGTRQLPSRQQDGSVRQRSKRHRKIRGPTKDHKIQIYFNITSIPLPLSRNDSVEVANQKRLLRTLEQLTNRLKRTLAKQPLSSYHVSSEMIISDPKSLESKKASLFCRPGSVLKGRMCQCPVGTYFSLEHNECESCWLGSYQDQEGQLECKSCPEGTSTAYLHSRSITECKQCKPGSHSLNGLEICESCPLGHFQPGYGARKCLVCSGGTSTVTRGAVDETECGPCMAGHFSRTGLVPCYPCPRDYYQPDHGRSYCLSCPFYGTTTITGASSIQRCSFSSSFLAKEESVTPAPEVEVIEDYQVSSQVFHECFLNPCQNKGTCEEVGAGYVCTCMPGFTAKCEVDINECDSAPCQNGGLCRDGMGDFQCQCKPGFLSLCEGQVNECLSSPCLNEGLCVDEVNKFSCSCAEGFTSLCELEINECLSSPCLNGGVCEDLTGDYSCNCAVGFSGDRCEINIDECYSSPCLNGGSCIDDVNSFCECVEGFRGRLCEVDVDECNPNPCVNGASCFDGLGSYTCRCLPGFNGSRCETMSSAFNLDFEVSGIHGYVMMDGVMPALTEITCSFWMKSTDITNYGTPVSYAVEGSDNAFLLIDYNWVLYVNGKERITDCPAVNTGQWYHIGVSWRSWDGDWRVYINGKPSDGGKGLSVGTTIPGGALVLGQDQDQRGEGFNPVESFVGSISQLNIWDRVLTPQQIKVLASSCPASHVTHRGNVLAWPDFLSGVAGRVKVNLNSIFCACPKLESAVSHLHASSVEVSPGAQVHLSCDPGFYLVGEPVLQCQNKGEWSHLLPSVSCGPPRPLENGLYQGSDFQAGSSVVYRCNVGFYLLGDAKVHCSNSGQWGGHPPACLVDECALGSDCDDHASCQNTEGSYSCTCIHPYTGDGKNCTPVKCQNPGSPEFGHRDGSSFLVGGAVAFGCDSGYELIGSSRLHCLESGTWDHPVPYCRLSCPTPSVPENSVMTGNNFTFGSKVTFCKNGFLPQIPYEFHCLSTLRWSGAPPICYPVTCGGPPAVAHASYVLDTNTYLSTIKYTCVEGYPQGSMEVVCEATGEWSRPIPRCVSILCNEPPALKDAVIVGENYEVGNKVHYVCKEYTLIGPETRECLPTGQWTEGFLQCVPRSCGPPPAVDHAVPHETHKLFGDTASYYCTDGYTASNNSKMVCNAQGVWVAPDGMEAPRCIANFCLRPPEVSHAILDSVNKPKYSSNTEVSYKCEEGFMLNTTATLRCLLGGTWEPSPFEIGCVPVRCSKPESIDRGYVRGTNYSFGAVVAYSCEAGYLIRGEKRRICKANGEWGGSLPTCVPVSCSSPPVRTRYTFNNKVTYACNAGYKLVGGPERVCQANRQWSNNDPPICVLLTCDPPPAVVHGHYKGSTFRVGSKVEYFCDEGYELGGNPVSTCLKNGKWDQPNPPRCSPVKCPEPPLEESHLVLKSLYSESGTVELSCEDGYILEGANVLRCTPSQEWNDTFPVCKEVFCGPPPDVTFGAPNNTSHSFPFSSVITYTCMGGFTFRKEDSVSCLASSRWSTPYPECIPVECPQPEEISNGFVDVQGLMYLSKALYSCRTGYNLVGDSTVLCGDRGTWIGGVQSCRPVECSIPKQIASGKVVYTKLQYGHSATYSCHRGFRLRGPETLKCSADGEWDSEPPVCTQISCTLPQPIENGFVEGQDHSFGVTIFYSCFPGFQLVGQDHLTCEETGWSSSVPMCVPSDCGLPPHIDFGDYVRVAESDTAPKDTIPASPLDLNFLHGTLIEYRCHQGYDISRHTRLVCQEDGGWNGTAPSCVPTECELPSNPQHGSINVTYAPVGSVVSYSCEEGYELVGEPIRQCVSGRLWTGDEPVCRPVSCGDPGVVANCLTHGRDFVYPEVLHFECHPGFVLKGTDTIACQADGNWNSQTPRCEPVSCGPPTVPSDLTVQGKEYTYNKEIELRCQPGFILKGTSKSVCQADGTWSHAYNSCVPAHCGRPSPIPHGRVLGSETGFNRKLKYECDEGYKLIGDPTVVCQSDGLWDKPPPRCDIISCDPPEDISHGFVNGSGFNYNNVVEYVCFEGYEVVGDPILRCSAQGLWVGSVPQCQPCACSPPEVQFGTVTGPGRACGDRVQFTCDQGYKLLGPSEAVCEKGGVWSPGVPVCGRGRCSIAPPVVPNAVLQGGTTTFPETVTYMCRPGYLLKGSSLLSCGKDGRWGEPRIRCEPVNCGKPPAVAHGQVVGDVFTFPNQVTYCEDGYKLATSRASLSCQSDGTWSKHTIQCLPAPCLLPTNISIPHLQINGRELTPVGGTITLSCPTGFYLQGSALAECQVMCEKPSALLHGVMEGDSYTYGDFVLYSCLPGFDLKGESIQTCQGDGTWSGTQPVCVSASSCGPPPFVKNAQIQTGGASLQNVTYVCNEGQQLDGPKTLTCLANGTWSMPPPTCAVRGCNSPKQVLHGRVQEHNLSSGRALEFLCDRGYSLVGDGLVMCMGGNTWSSTFPIKSCPPPPWWIKHTNSGGSQPEFHVGQAVRASCPKGQQVKGGGTITCRPDQTWTPLSSVCDVSCGPPLHVANGLVRGAVFQFGDVAVYSCFSGYTMEGVDRSRCQENGTWTPPPTCRRCWLQCQNGGVCQRPNICACTEGWMGRLCEEICILPCLNGGHCVAPYQCECPSGWTGTRCHTVCSSPCLNGGRCIRPNRCSCTPGWSGHNCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMY4</accession>
    <name>A0A3B5LMY4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Frizzled-3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor Fz/Smo family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027896864.1">
        <property type="nucleotide sequence ID" value="XM_028041063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMY4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012342"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012491.1">
        <property type="protein sequence ID" value="ENSXCOP00000012342.1"/>
        <property type="gene ID" value="ENSXCOG00000009330.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114159030"/>
    <dbReference type="CTD" id="565921"/>
    <dbReference type="GeneTree" id="ENSGT00940000156491"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042813">
        <property type="term" value="F:Wnt receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033564">
        <property type="term" value="P:anterior/posterior axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071679">
        <property type="term" value="P:commissural neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021754">
        <property type="term" value="P:facial nucleus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035567">
        <property type="term" value="P:non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000036">
        <property type="entry name" value="frizzled-3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000006">
        <property type="entry name" value="Frizzled-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000539">
        <property type="entry name" value="Frizzled/Smoothened_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF144">
        <property type="entry name" value="FRIZZLED-3 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01534">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00489">
        <property type="entry name" value="FRIZZLED"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01330">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 21" id="KW-0732">Signal</keyword>
    <keyword evidence="16" id="KW-0807">Transducer</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017419987" description="Frizzled-3" evidence="21">
        <location>
            <begin position="18"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="291"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="376"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="420"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="22">
        <location>
            <begin position="27"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="23">
        <location>
            <begin position="202"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="547"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="554"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="630"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="32"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="40"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="678" mass="76047" checksum="EFA438C518226EC5" modified="2018-12-05" version="1">MDLLWVLSVSMLAACVAIPMDAAAGSHSLFTCEPITLRMCQGLPYNSTFMPNIMNHYDQQTAALAMEPFHPMVNLQCSPDLRMFLCAIYAPVCTEYGRMTLPCRRLCLQARSDCYKLMDMFGVSWPEEMDCNRFPDCDEPYPRPADLLSTSDSTESPVSVQRDYGFWCPRELKIEPELGYSFLGVRDCSAPCPNMYFTREELTFARYFIGVVSIVCLSATLFTFLTFLIDVSRFRYPERPIIFYAVCYMMVSLVFFLGFLLEDRVSCNAASPGKFRASTVTQGSHNKACTLLFMVLYFFTMAGSVWWVILTITWFLAAVPKWGSEAIEKKALLFHACAWGVPGVLTVTLLAMNKIEGDSVSGVCFVGLYNLTALRWFLLAPLGLDVVVGVVLLLAGIAALNRVRMEIPLEKENQEKLVKFMIRIGVFSVLYLVPLLTVVACYLYESSYRTVWETTWVQEKCRDYHIPCPYKVESTSRPDLALFLIKYLMMLIVGIPSVFWVGSKKTCFEWASFFHGKRRKDGMVNDSRQVLQEPDFAQLLYRDPNTPIVRKSRGTSTQGTSTHASSTHLAMLDEPPSASTSRAGSVHSARSKMSSYHGSLHRSRDGRYTGSSMPRLSDQSRHCSTNRLDGLSRHDSALRLDSQSRHSSTRDLSGAAQAVLGVPGNGIHRILDEDGAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPP6</accession>
    <name>A0A3B5LPP6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPP6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009814"/>
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        <property type="protein sequence ID" value="ENSXCOP00000009814.1"/>
        <property type="gene ID" value="ENSXCOG00000007450.1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5045270930" evidence="1">
        <location>
            <begin position="25"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="312" mass="34680" checksum="ECC055F6579D6BFC" modified="2025-10-08" version="2">MRRSNWCLVSQRLCLSLLGRLAGSERERLLKQADISSPGDSAERREEMEAAMMQVCANIMEFCQTLLLQSSSQAQFSICLFSPSGSEPAGRDGARSDLSSSVPSITHSRVPSLGLVLYLLKNSAADFFQFHQSHRQSLGKLQSLEQLPPEELKELCQGLVSGPGGVEKISSVQRSLLARRRLVQFINNRAKLLAFCSYVIETCLFVLWRHLEYYLLHCTPSDPKDSLLPGSSLFRTRLADGETAGGGRGIGLSRVSQQDLDLLKGDMAAGFGEALQRKLLEVEGQYSQVRSRYTFIQALVRRIRGLLRQPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQB1</accession>
    <name>A0A3B5LQB1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQB1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013125.1">
        <property type="protein sequence ID" value="ENSXCOP00000012965.1"/>
        <property type="gene ID" value="ENSXCOG00000009817.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="75" mass="8958" checksum="37798DFFE46D2552" modified="2018-12-05" version="1">MLMRKLLEKREFQQDSNSKRTNKSGQFWNFNPIENLRAVQSSMSRIHVRKSQRMIDSMSCRFEASFIEHGSEAKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRD7</accession>
    <name>A0A3B5LRD7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRD7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011971"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012109.1">
        <property type="protein sequence ID" value="ENSXCOP00000011971.1"/>
        <property type="gene ID" value="ENSXCOG00000009063.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010968"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034164">
        <property type="term" value="P:negative regulation of toll-like receptor 9 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037847">
        <property type="entry name" value="GRAMDC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37402">
        <property type="entry name" value="GRAM DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37402:SF1">
        <property type="entry name" value="GRAM DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="420"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="33"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="427"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="451"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="506" mass="55985" checksum="DBB227660F217FDC" modified="2018-12-05" version="1">MSVGKGKKLSHTSDTENSDDVVTKMRFLPREQEKITLLENQFLQKELKKLKDEPLIDSLKQELEKELSKRTELEQKVIKLEVQDSPSQPGTTAAKMKIRDRLRAFKEKLSFHPKAIVEREDPLSPIKMTKNMRRLERDFRPVAHFFRNLSALSSWRSVHMTFCFFCVYMCTVWYSWTIPSILLLMILHLSINYLTSKGWRIPRSIIPDVCEPGEHLKGNPNILQMFHLAFNSAQKTQKLFGNMADILEKIKNLCMWVRPELTVKSYVGLWLVFIFSCVFPYQLLGFIIGVSVGIRFFIIDLIFERCSKLQQRFDAPYNFWTNLPTDLQLRELRNTFLKKHPVHFNYYLISESSVALNLSLFLLTPVRTLTCLSNVSSKNSLISSRVYKSPLDVCESAIETLSESLSYSMSSTCAGGAADWTPAAPPSGNTTASAAPPSGNATASAAPPSGNTTASAAPPSGSATASAAPPSGNTTASAAPPSGSATASAAPPSGSATASATTQPTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LT66</accession>
    <name>A0A3B5LT66_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ATP synthase F(0) complex subunit a</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">F-ATPase protein 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Proton-conducting channel, ATP synthase F(0) complex subunit a</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>H(+)(in) = H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34979"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the ATP synthase complex composed at least of ATP5F1A/subunit alpha, ATP5F1B/subunit beta, ATP5MC1/subunit c (homooctomer), MT-ATP6/subunit a, MT-ATP8/subunit 8, ATP5ME/subunit e, ATP5MF/subunit f, ATP5MG/subunit g, ATP5MK/subunit k, ATP5MJ/subunit j, ATP5F1C/subunit gamma, ATP5F1D/subunit delta, ATP5F1E/subunit epsilon, ATP5PF/subunit F6, ATP5PB/subunit b, ATP5PD/subunit d, ATP5PO/subunit OSCP. ATP synthase complex consists of a soluble F(1) head domain (subunits alpha(3) and beta(3)) - the catalytic core - and a membrane F(0) domain - the membrane proton channel (subunits c, a, 8, e, f, g, k and j). These two domains are linked by a central stalk (subunits gamma, delta, and epsilon) rotating inside the F1 region and a stationary peripheral stalk (subunits F6, b, d, and OSCP). Interacts with DNAJC30; interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase A chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LT66"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014015"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014189.1">
        <property type="protein sequence ID" value="ENSXCOP00000014015.1"/>
        <property type="gene ID" value="ENSXCOG00000010609.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005568"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00310">
        <property type="entry name" value="ATP-synt_Fo_a_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.220">
        <property type="entry name" value="ATP synthase, F0 complex, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000568">
        <property type="entry name" value="ATP_synth_F0_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023011">
        <property type="entry name" value="ATP_synth_F0_asu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045083">
        <property type="entry name" value="ATP_synth_F0_asu_bact/mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035908">
        <property type="entry name" value="F0_ATP_A_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01131">
        <property type="entry name" value="ATP_synt_6_or_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11410">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11410:SF0">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00119">
        <property type="entry name" value="ATP-synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00123">
        <property type="entry name" value="ATPASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81336">
        <property type="entry name" value="F1F0 ATP synthase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00449">
        <property type="entry name" value="ATPASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-0138">CF(0)</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="61"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="117" mass="12421" checksum="E4567C236C5F93EE" modified="2018-12-05" version="1">LATVVLGLCTQPTQALGHLLPEGTPTPLIPVLIIIETISLFIRPIALGVRLTANLTAGHLLIQLISSATSVLLSVLPAVATFTITLLFLLTLLEVAVAIIQAYVFVLLLSLYLQENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUI2</accession>
    <name>A0A3B5LUI2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IFI6/IFI27 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUI2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014695.1">
        <property type="protein sequence ID" value="ENSXCOP00000014510.1"/>
        <property type="gene ID" value="ENSXCOG00000011005.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097193">
        <property type="term" value="P:intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001836">
        <property type="term" value="P:release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009311">
        <property type="entry name" value="IFI6/IFI27-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038213">
        <property type="entry name" value="IFI6/IFI27-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16932:SF38">
        <property type="entry name" value="INTERFERON ALPHA INDUCIBLE PROTEIN 46-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16932">
        <property type="entry name" value="INTERFERON ALPHA-INDUCIBLE PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06140">
        <property type="entry name" value="Ifi-6-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="104" mass="9294" checksum="D52D3BBFF4DDD396" modified="2018-12-05" version="1">CVSGKILVITGGSVGAVALTPVALGAIGFTSAGIAAGSFAASMMSVAAISNGGGVAAGGLVAVLQSAGAAGLSGAASAVVASVGAGVGAAVGRLAGKTHKEKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUV0</accession>
    <name>A0A3B5LUV0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane protein 14A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TMEM14A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUV0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015763"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015958.1">
        <property type="protein sequence ID" value="ENSXCOP00000015763.1"/>
        <property type="gene ID" value="ENSXCOG00000011913.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158206"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070453">
        <property type="term" value="P:regulation of heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1740">
        <property type="entry name" value="Transmembrane protein 14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005349">
        <property type="entry name" value="TMEM14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044890">
        <property type="entry name" value="TMEM14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 14, 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668:SF11">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 14A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03647">
        <property type="entry name" value="Tmemb_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="102" mass="10849" checksum="2D1C1F26CDA23F4E" modified="2025-10-08" version="2">MAMDWIGFGYAAAIIFGGFMGYKRKGSVMSLIAGLVFGGLSAYGAYNVSNDPKDILVLLASSGLLAVIMGMRYKKSGKLMPAGIMTGLSLLMVFRLLLLTIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVL6</accession>
    <name>A0A3B5LVL6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">DNA replication ATP-dependent helicase/nuclease</fullName>
            <ecNumber evidence="21">3.1.-.-</ecNumber>
            <ecNumber evidence="21">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Key enzyme involved in DNA replication and DNA repair. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break (DSB) repair by mediating the cleavage of 5'-ssDNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20 21">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="21">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="21">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 21">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="21"/>
    <dbReference type="EC" id="3.6.4.12" evidence="21"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVL6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016003"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016202.1">
        <property type="protein sequence ID" value="ENSXCOP00000016003.1"/>
        <property type="gene ID" value="ENSXCOG00000012086.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00780000122010"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033567">
        <property type="term" value="P:DNA replication, Okazaki fragment processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071932">
        <property type="term" value="P:replication fork reversal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18041">
        <property type="entry name" value="DEXXQc_DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22318">
        <property type="entry name" value="DNA2_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000721">
        <property type="entry name" value="DNA replication ATP-dependent helicase/nuclease DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000789">
        <property type="entry name" value="DNA replication ATP-dependent helicase/nuclease DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000915">
        <property type="entry name" value="DNA replication ATP-dependent helicase/nuclease DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026851">
        <property type="entry name" value="Dna2/JHS1_DEXXQ-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048459">
        <property type="entry name" value="DNA2_Rift"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014808">
        <property type="entry name" value="DNA_replication_fac_Dna2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF433">
        <property type="entry name" value="DNA REPLICATION ATP-DEPENDENT HELICASE_NUCLEASE DNA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08696">
        <property type="entry name" value="Dna2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21123">
        <property type="entry name" value="Dna2_Rift"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 21" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 21" id="KW-0227">DNA damage</keyword>
    <keyword evidence="17 21" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 21" id="KW-0235">DNA replication</keyword>
    <keyword evidence="15 21" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11 21" id="KW-0347">Helicase</keyword>
    <keyword evidence="10 21" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 21" id="KW-0408">Iron</keyword>
    <keyword evidence="14 21" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="22" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16 21" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="19 21" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5 21" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18 21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="DNA replication factor Dna2 N-terminal" evidence="23">
        <location>
            <begin position="83"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2 rift barrel" evidence="26">
        <location>
            <begin position="460"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="24">
        <location>
            <begin position="618"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="24">
        <location>
            <begin position="729"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="25">
        <location>
            <begin position="796"/>
            <end position="1012"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU367041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF08696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF21123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1054" mass="118120" checksum="7D1FB0878ADA89F2" modified="2018-12-05" version="1">MRRELVLSLWLGYCCHSNCLLRWLSVTTFPKCDVMRTKYIMILFFFFFFLPLGSCVLSYFYSFVRCASASKCRIFSSLCRELTPVCRGDVVHLEGRSEGGAWLVDRERGFLVLQPDSLISGTSISNSIRCMRRAVLGELFKRFDGGSKQMLNGTMVHEVFQRAASAKDFSLQKLSQLADEALRNHRYLGDMFALGVSQEEMQQELHDYLPSLEHWANEYLCSSTPKPIGSKIRCQAPPAVFTLTELADIEENVWSPRFGLKGKIDVTAKVRIQRSRNGSNRVCEEKTVPLELKTGRESNSIEHRSQVILYTLMSMERYGPEIGLLLYLKTGNMHPVAAGHMDHRELLKLRNSLVHHINHFVEKGADRSRLSRLPDILTSRQTCQYCPQKLNCALYERVVDSTSADVGEDTVQAFLQQETSHLTHPHLDYFAHWLLLCSLEASTMEAKSGRKRVWLQSVLESGSCVGNLQLSDSVTAQSEGVFVHRFLRSAADSQQAANHCGLTSGDRVVVSDQEGRLIGLATGYLKGVNATSVSCTLDRWALRSQLILGILFRLDSDEGVVGLSTHLSNLSRLMENSGDSERLRELVVDLRPPEFITNLSSVLPREAKDTVANILKGLNKPQKQAMKKVLLSKDFTLIVGMPGTGKTTAICTLVRILHACGFSVLLTSYTHSAVDNILLKLKRFRVGFLRLGQGQKVHPEILPFTEESVRRKDVHTLTELQQLYCKELVVATTCMGIKHPIFTRRRFDFCIVDEASQISQPICLGPLFYAKRFVLVGDHQQLPPIVQNQEARSFGMDESLFKRLELHSEAVVQLNVQYRMNSQIMSLSNSLMYDGRLECGSERTASALLTLPSLASVQSEVTSRSETRPQLDLSWLQAALLPSNPVCFLDCSVPALESVEQGGVSNRTEAALIHMMLSLLIKAGCKPGDIGVIAPYRQQLKCISALLQSSAFSGVEVNTVDRYQGRDKSLIVLSFVRSTAEEGNLGELLKDWRRLNVAVTRAKHKLLMVGSAATLRRYPPVEKLLQHLLSQVEIQFLLFFSDLPASAPWRPPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LW91</accession>
    <name>A0A3B5LW91_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">TGFB-induced factor homeobox 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TALE/TGIF homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LW91"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015140"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015330.1">
        <property type="protein sequence ID" value="ENSXCOP00000015140.1"/>
        <property type="gene ID" value="ENSXCOG00000011475.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155230"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048854">
        <property type="term" value="P:brain morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021797">
        <property type="term" value="P:forebrain anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046530">
        <property type="term" value="P:photoreceptor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048385">
        <property type="term" value="P:regulation of retinoic acid receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072091">
        <property type="term" value="P:regulation of stem cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017015">
        <property type="term" value="P:regulation of transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070654">
        <property type="term" value="P:sensory epithelium regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000059">
        <property type="entry name" value="TGFB-induced factor homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="34"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="36"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="99"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="197"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="118"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="165"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="199"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="263" mass="28548" checksum="5D1FA249AB5B12E1" modified="2018-12-05" version="1">MKTKKGLTMLSGSETDDEDSVDAPLDLSSSTGSNGKRKRRGNLPKESVQILRDWLYEHRYNAYPSEQEKALLSKQTQLSTLQVCNWFINARRRLLPEMLRKDGKDPNQFTISRKGSKAGDSFSDSSQSPKHSSPAAGAEEACDKRALHPSSFDPSIPSVLRKPVSHSMSSPVSSSPSPGLIPNRPSVICHTTVTTAMQASPTASHPSASTSMEGNLSPRSGLFNTPPPTPPDLTQDFSGFQLLVDVALKRAAEMELQAKQLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWY6</accession>
    <name>A0A3B5LWY6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Methyltransferase-like 26</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0585 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWY6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016438"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016645.1">
        <property type="protein sequence ID" value="ENSXCOP00000016438.1"/>
        <property type="gene ID" value="ENSXCOG00000012402.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010750"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010342">
        <property type="entry name" value="DUF938"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20974:SF2">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20974">
        <property type="entry name" value="UPF0585 PROTEIN CG18661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06080">
        <property type="entry name" value="DUF938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="253" mass="28340" checksum="8A93C36E3AABDD4C" modified="2018-12-05" version="1">MSGCLNCFSAPLVKEAQLGEFRSPKGLPRWLHKLYSITRRLNPGFGTMLNAAAAERNKEPILSVLRDSVNTERPLQALEISSGTGQHVVHFAQTLRNITWQPSEYDRQSLASIEAYRSHYQLHNVKPAVYLDASRPHQGWAGIQPESLDLVLNINMIHISPLSCTEGLFRGAGVLLKPQGLLLTYGPYAVNGQITPQSNVDFDYSLRQRNPEWGLKDISFLNSVAQKNGLFLEKIVEMPANNKCLLFRKESLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LX81</accession>
    <name>A0A3B5LX81_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">KIAA1549 like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LX81"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015505"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015698.1">
        <property type="protein sequence ID" value="ENSXCOP00000015505.1"/>
        <property type="gene ID" value="ENSXCOG00000011726.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063472"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024606">
        <property type="entry name" value="KIAA1549"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21590">
        <property type="entry name" value="SEA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21590:SF3">
        <property type="entry name" value="UPF0606 PROTEIN KIAA1549L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12877">
        <property type="entry name" value="KIAA1549"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="402"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="525"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="408"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="451"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="550"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="577" mass="63256" checksum="2CBE9D46AACEB3A6" modified="2018-12-05" version="1">QVETVFGSPNMTVAYHVTGGETVYPPSVVAEGLMSYGRDKLIADLRQYLPMVTALPLPVALWRSSPATGFQLKTLQFVGSGDDPRSCRFLQTMEQRLEKVFSEAQAKVLSAKSRLAVQILSVSQTPGSPAVSLVYTVKNGTVFLNGTVASNLLGQFSAELVGYFLFYPPLIIAELEYHNLNTSVETRDFWVVTIQDVDNASLEGQYQSFASLMEQRLADLFVLAGQQGTRFRRATTVGSFTVQMVSIRRASGLKNPAEMTYYVQHNGIPLSGTSAAKVLNTVDSQTMALTLGYFVQVQAEVVKNPPNNLWIIAAVLAPISVVTLIIIIITAVLCKKNKNDFKADAIGNLNPRAKPVQGFDYAKQHLGQQGGDEETLPVSQDTLVMSLQIRDTSLSLEKALQQDGTANKKSHTADKHKKLPSEDGSVISDESEKLNSKRGLTVPKVTAQQKSTKEEARRRDPYDTSSGSLQLISIKPVTAQTNCSHPAFSDRSQDSAIVNGEQVSLALKQKSDIEHYRNKLRLKAKKKGYYDFPSTNGSSSSGGRALVHKQRSDYEREVGEHGRPLEPDDEQGSTYVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXM0</accession>
    <name>A0A3B5LXM0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXM0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012569"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012720.1">
        <property type="protein sequence ID" value="ENSXCOP00000012569.1"/>
        <property type="gene ID" value="ENSXCOG00000009295.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164025"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01385">
        <property type="entry name" value="CLAUDIN14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="170" mass="18276" checksum="A8DD87EA391EC391" modified="2018-12-05" version="1">MASQGLQILGILLSFIGWLGSIFTCVLPMWRVTAFVGANIVTAQVIWEGLWMSCVVQSTGQMQCKVYDSMLALSQDLQAARAMVIISVIVGVFGMLMAIAGGKCTNSTPRICNPKDAIAVSVRSFLCAWPPTETSSAAKKFYASLPGWALAPVLTYKHLSCFEPRGGPHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZU7</accession>
    <name>A0A3B5LZU7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Calcineurin B homologous protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Tescalcin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Ruffle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the calcineurin regulatory subunit family. CHP subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZU7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013269"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013433.1">
        <property type="protein sequence ID" value="ENSXCOP00000013269.1"/>
        <property type="gene ID" value="ENSXCOG00000010062.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155845"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052490">
        <property type="entry name" value="CHP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46823">
        <property type="entry name" value="CALCINEURIN B HOMOLOGOUS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13405">
        <property type="entry name" value="EF-hand_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="101"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="212" mass="24331" checksum="F59770B16A177867" modified="2018-12-05" version="1">MLMINVFVSSVSTEQIKNLHMRFQQLCGNEKTLRKEHLENIPTLADNPIKTEIIKAFFDKRNQHNNELGSYEEIGFEEFLMVMSHFRPPSIKTSAEEKAAMRKRKVRFLFNMHDTDGDGKITLEEYRKVGIVEGLLSESESIGQEVARAIADAAMLEVASTNVPNMVREVTKITLALFLHFACPQILKDLEMESRMYVRFLDMNTANLRCGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M121</accession>
    <name>A0A3B5M121_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M121"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017107"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017327.1">
        <property type="protein sequence ID" value="ENSXCOP00000017107.1"/>
        <property type="gene ID" value="ENSXCOG00000012897.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="118" mass="13121" checksum="70D101F00564043C" modified="2018-12-05" version="1">MRKDSPWKQNVSLLARERLHGGSGQDVGSLPGVRLQDQVNRKLHLPAPPGVFMLVAGGIVAGIFLIFIEIAYKRHKDARRKQMQLAFAAVNVWRKNLQNWTTGSEREGSDPSLPTVPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M327</accession>
    <name>A0A3B5M327_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M327"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017842"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018066.1">
        <property type="protein sequence ID" value="ENSXCOP00000017842.1"/>
        <property type="gene ID" value="ENSXCOG00000013446.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161075"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005332">
        <property type="term" value="F:gamma-aminobutyric acid:sodium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF280">
        <property type="entry name" value="TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="167" mass="18759" checksum="41614E3C8DE333A4" modified="2018-12-05" version="1">CVELHDPSLVPVIALRDKQDPEEGVQPRGKWANNMEFILSMAGEIIGLGNVWRFPYLCYKNGGGVFLIPYCVFLFCCGIPVFFLETALGQYTSEGGVTAWRKICPMFEGFGIASQVIVIYLNIYYIVVLAWAIFYLINSFNIPLPWSSCTTSVTCKLHLYALSSFCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3A7</accession>
    <name>A0A3B5M3A7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pinin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="20">Found in a mRNA splicing-dependent exon junction complex (EJC). Found in a complex with SR proteins. Found in a mRNP complex with RNPS1. Component of the PSAP complex consisting of RNPS1, SAP18 and PNN. Interacts with PNISR, CTBP1, CTBP2, KRT8, KRT18, KRT19, PS1D/PNO40, PPIG, RNPS1, SFRS4 and SRRM2. Identified in the spliceosome C complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Desmosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pinin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3A7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018633"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018867.1">
        <property type="protein sequence ID" value="ENSXCOP00000018633.1"/>
        <property type="gene ID" value="ENSXCOG00000014023.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111160"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030057">
        <property type="term" value="C:desmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039853">
        <property type="entry name" value="Pinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006786">
        <property type="entry name" value="Pinin_SDK_MemA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006787">
        <property type="entry name" value="Pinin_SDK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12707:SF0">
        <property type="entry name" value="PININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12707">
        <property type="entry name" value="PINN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04696">
        <property type="entry name" value="Pinin_SDK_memA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04697">
        <property type="entry name" value="Pinin_SDK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="16" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0965">Cell junction</keyword>
    <keyword evidence="14 21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="18" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Pinin/SDK" evidence="24">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Pinin/SDK/MemA protein" evidence="23">
        <location>
            <begin position="138"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="90"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="277"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="175"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="277"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="314"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="22">
        <location>
            <begin position="327"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="367"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="401"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="461"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="483"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="524"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="557"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="595"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00025916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF04696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF04697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="648" mass="72694" checksum="65BEEBF83FDD1004" modified="2018-12-05" version="1">MAVAVRSLQDQLEKAKENLKNVEDNIRKLTGRDPNESPGQLRRLSGPMVGLGGGRGRGINLLRSLSDMGSSGPPAKQRDIEGALLLAGDQRARRDVRHDSDAEDDDDVKKPALQSSVVATSKERTRRDLIQDQTMDEKGKQNRRMFGLLMGTLQKFKQESNVSSEKQLKRRSEIEQKLEVQAEAERKKVENEKRELFEERRAKQTELRLLEQKVELAQLQEEWNSHNNHLVKYIRTKTKPHIFYRPGKMCSATQKLLEESTKKLNVFEERREAFAEHLSKMESRPRRQPNRDQDGSSASRMDRPAEGKPAGQVEKQRGKRGESRREEDEDEDEDEEEEREKEIGGGDAEEGEKEELVKIEEVEEEVMEVREEGSKEEAGEEQKEKSEKAASPESEEMEVEKEDKGKEVEVETDRKQESTEIQEEKTPDVQSCEAAVSGLEVPDSSDEPPQSLAAGEPAAQSPVTETASPSQEPQKASAALAQDPTSPTQDNMATESSVQDSTAENQTEERKSKEDEEEGPQKLPDSKEVDAPAKEEGEEQGRGRKKEKDAKKHRSRSNSSSSSSSGSSSSGSSSSSSGSSRSSSSSSSSSTSSSRSRDSDKRKRRPSDRGRDKKKEERSRQKSGGRDSAERRKRRSEEKKGRPSRSDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M463</accession>
    <name>A0A3B5M463_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome c domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 11">Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="11">Binds 1 heme group per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the cytochrome c family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027863440.1">
        <property type="nucleotide sequence ID" value="XM_028007639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M463"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018943"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019180.1">
        <property type="protein sequence ID" value="ENSXCOP00000018943.1"/>
        <property type="gene ID" value="ENSXCOG00000014246.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114138409"/>
    <dbReference type="KEGG" id="xco:114138409"/>
    <dbReference type="GeneTree" id="ENSGT00940000157883"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.760.10:FF:000001">
        <property type="entry name" value="Cytochrome c iso-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="entry name" value="Cytochrome c-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002327">
        <property type="entry name" value="Cyt_c_1A/1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11961">
        <property type="entry name" value="CYTOCHROME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00604">
        <property type="entry name" value="CYTCHRMECIAB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="Cytochrome c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 9 11" id="KW-0349">Heme</keyword>
    <keyword evidence="8 9 11" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome c" evidence="12">
        <location>
            <begin position="2"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="104" mass="11455" checksum="BD1BA2CC0B74510A" modified="2018-12-05" version="1">MGDVEKGKKVFVQKCAQCHTVEAGGKHKVGPNLHGIFGRKTGQAEGYSYTDANKSKGIVWSDDTLMEYLENPKKYIPGTKMIFAGIKKKGERQDLVAYLKSSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4Z3</accession>
    <name>A0A3B5M4Z3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Inhibin subunit beta C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">INHBC</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4Z3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018440"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018672.1">
        <property type="protein sequence ID" value="ENSXCOP00000018440.1"/>
        <property type="gene ID" value="ENSXCOG00000013881.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160065"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19406">
        <property type="entry name" value="TGF_beta_INHBC_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000005">
        <property type="entry name" value="Inhibin beta A chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000028">
        <property type="entry name" value="Inhibin subunit beta C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF6">
        <property type="entry name" value="INHIBIN BETA E CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0339">Growth factor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017311475" evidence="9">
        <location>
            <begin position="35"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="10">
        <location>
            <begin position="266"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="383" mass="43226" checksum="29C2F3589BAF8E39" modified="2018-12-05" version="1">MLNFSLCWRMTPSFSFISLLLLATLLLKGLRVSGSPACASCGLPAMEKDAEQKMMIEIAKQHLLEKLHLKERPNITQTVPKVALLTALRKLHSGRVRQDGTLELENSLPTKDQGYEIVSFADISDSDSGDKASLSLTFQFLQERGKSIQVLQSSLWIYAKLSEDPHRSSRLLARVFLSTDEETSDSNRTLVIEKMIDVKESNWHTFPITHTLQTFLDGGQRRLRLEVSCEEGERNLCSFNGSTDKANQPFMVAQVRLRDDHSKHLIRKRSLRCGDEVTVCCKRDFYIKFKDIQWDEWIIAPEGYHMNYCMGQCPQHLAGSPGIASSFHATVFNQLKVNGINTATASCCIPTERRPLSMVYFNSQHSIVKTDVPDMIVESCGCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6R1</accession>
    <name>A0A3B5M6R1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Nanos homolog 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the nanos family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6R1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019187"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019424.1">
        <property type="protein sequence ID" value="ENSXCOP00000019187.1"/>
        <property type="gene ID" value="ENSXCOG00000014426.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183135"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043186">
        <property type="term" value="C:P granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036099">
        <property type="term" value="P:female germ-line stem cell population maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008354">
        <property type="term" value="P:germ cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001555">
        <property type="term" value="P:oocyte growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.30:FF:000001">
        <property type="entry name" value="nanos homolog 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.30">
        <property type="entry name" value="Nanos, RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008705">
        <property type="entry name" value="Nanos/Xcar2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038129">
        <property type="entry name" value="Nanos_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024161">
        <property type="entry name" value="Znf_nanos-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12887">
        <property type="entry name" value="NANOS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05741">
        <property type="entry name" value="zf-nanos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51522">
        <property type="entry name" value="ZF_NANOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nanos-type" evidence="10">
        <location>
            <begin position="142"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="98"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="99"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="210" mass="23312" checksum="A08F9583199C691C" modified="2018-12-05" version="1">MNGTVSGLFHHLSRFMESNSESFQPWRDYMGLSETVREILSRNSTEESVLPHLKAHHSQPDHFSGTLGSMRMNAESCESLLSGSASVLNPEDMFRFAPQSASADPLQTPTTGSSCRPKDRRSTASRFKAPAPEPPARPSSKFCSFCKHNGESELVYGSHWLKNQAGEVVCPYLRQYVCPLCGATGARAHTKRFCPKVDSSYSSVYTKTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6U5</accession>
    <name>A0A3B5M6U5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6U5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019968"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020215.1">
        <property type="protein sequence ID" value="ENSXCOP00000019968.1"/>
        <property type="gene ID" value="ENSXCOG00000014996.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00790000123099"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000380">
        <property type="term" value="P:alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12648">
        <property type="entry name" value="RRM3_UHM_PUF60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000133">
        <property type="entry name" value="poly(U)-binding-splicing factor PUF60 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051974">
        <property type="entry name" value="PUF60_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034212">
        <property type="entry name" value="PUF60_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47330:SF1">
        <property type="entry name" value="POLY(U)-BINDING-SPLICING FACTOR PUF60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47330">
        <property type="entry name" value="POLY(U)-BINDING-SPLICING FACTOR PUF60-B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="31"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="128" mass="14659" checksum="D466C36CC0157197" modified="2018-12-05" version="1">MPLSEQEHMSISGSSARHMVMRKLLRKQESTVMVLRNMVGPKDIDDDLEGEVTEECGKFGQVRRVIIYQERQGEEDDADVIVKIFVEFSEAAHRNRAIQALNNRWFGGRKVAAEVYDRDRFDASDLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7B1</accession>
    <name>A0A3B5M7B1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UDP glucuronosyltransferase 5 family, polypeptide F1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7B1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015878.1">
        <property type="protein sequence ID" value="ENSXCOP00000015684.1"/>
        <property type="gene ID" value="ENSXCOG00000011850.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165787"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000203">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF52">
        <property type="entry name" value="UDP GLUCURONOSYLTRANSFERASE 5 FAMILY POLYPEPTIDE B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017443534" description="UDP glucuronosyltransferase 5 family, polypeptide F1" evidence="4">
        <location>
            <begin position="31"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="318" mass="36531" checksum="BBAE0B604769A8E5" modified="2018-12-05" version="1">GSQTPVPKAMYQLTLLTLAVLLCSSSLVNGGKVLVFPVDGSHWVNMNIIIEQLHARGHEVTVLRPFDSWHIKPDSLHYKAITVNSSSGFSEQSFGLFVNRTLELRREGPSFRTHISIVCNLLSQSYLMHEQVLQMTEVIFEDKVLMQSLRDEKFDLVLTDPAFGGGVFLGHRLGLPLVFNVRWTIQVEGHFTIAPSPLSYIPVPGSELTDRMTFIQRVNNFLIYIVTCIQFWYVVEPLYPEFVHRHFGKDVDYIELFQSADIWLMRNDFTFEFPRPTMPNIIYMSGFQCKPSKPLPKQLEEHVLLKRFQDSAKIAVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7G7</accession>
    <name>A0A3B5M7G7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">hydroxymethylglutaryl-CoA lyase</fullName>
            <ecNumber evidence="3">4.1.3.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(3S)-3-hydroxy-3-methylglutaryl-CoA = acetoacetate + acetyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:24404"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="4.1.3.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate metabolism; (S)-3-hydroxy-3-methylglutaryl-CoA degradation; acetoacetate from (S)-3-hydroxy-3-methylglutaryl-CoA: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMG-CoA lyase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.4" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7G7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015734"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015928.1">
        <property type="protein sequence ID" value="ENSXCOP00000015734.1"/>
        <property type="gene ID" value="ENSXCOG00000011887.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159467"/>
    <dbReference type="UniPathway" id="UPA00896">
        <property type="reaction ID" value="UER00863"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004419">
        <property type="term" value="F:hydroxymethylglutaryl-CoA lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046951">
        <property type="term" value="P:ketone body biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07938">
        <property type="entry name" value="DRE_TIM_HMGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000038">
        <property type="entry name" value="Hydroxymethylglutaryl-CoA lyase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043594">
        <property type="entry name" value="HMGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004283">
        <property type="entry name" value="PRK05692.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42738:SF16">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COA LYASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42738">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="7">
        <location>
            <begin position="2"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="295" mass="31457" checksum="699F5F9FDD035B9A" modified="2018-12-05" version="1">PSVAPTLPVRDVVAMTTEIVPTRVKIQLIDMLSTTGLSVIEATSFVSSKWVPQMADHTDVVKGIQRVPHVQYPVLTPNIQGFQNAVAAGATEVAVFGSASETFCRRNINCSISESMLRFEEVISTAKDRQIPVRGYVSCALGCPHEGHIEPSQVAEVAKKLYEMGCYEISLGDTIGVGTPGSMFKMLQKVMNDVPISALAVHCHDTYGQALPNILTALQMGVSVVDSAVAGLGGCPYAQGSSGNVSTEDVLYMLHGMGIETGVNLDKVIETGDFICNALGRRTNSKVAQARSRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7V7</accession>
    <name>A0A3B5M7V7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7V7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016066.1">
        <property type="protein sequence ID" value="ENSXCOP00000015869.1"/>
        <property type="gene ID" value="ENSXCOG00000011987.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000267447"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF18">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Immunoglobulin" evidence="6">
        <location>
            <begin position="30"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin V-set" evidence="5">
        <location>
            <begin position="40"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="26807" checksum="7D968933DACF45EF" modified="2018-12-05" version="1">NDLLSHRRRTSRCVSCGVWHTDNDNGLYGSFEFVAAAGESLTLRCTFKPRNSFPKYMLKEYVKLPDNAPEFSKDRFDAKVNNSAFDLKIHNLHPSDSAVYYCALQPTVTGNTRTLYKNLQFSTTATRGPHKPGSSVALQTNRTINFISSWSEQTAGGASWRFMAVDEFLSVTNEGGVKIPYCCPSSYVRSLVYFLPSYVRLPVPQTPGDISHFLKPKACEVMGQSESSSIKLTEDEYRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8N0</accession>
    <name>A0A3B5M8N0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">GB1/RHD3-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M8N0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017626"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017849.1">
        <property type="protein sequence ID" value="ENSXCOP00000017626.1"/>
        <property type="gene ID" value="ENSXCOG00000012464.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155710"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098826">
        <property type="term" value="C:endoplasmic reticulum tubular network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990809">
        <property type="term" value="P:endoplasmic reticulum tubular network membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.420:FF:000001">
        <property type="entry name" value="Atlastin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000314">
        <property type="entry name" value="Atlastin-2 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.420">
        <property type="entry name" value="AHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003191">
        <property type="entry name" value="Guanylate-bd/ATL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036543">
        <property type="entry name" value="Guanylate-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015894">
        <property type="entry name" value="Guanylate-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10751">
        <property type="entry name" value="GUANYLATE BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02263">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02841">
        <property type="entry name" value="GBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48340">
        <property type="entry name" value="Interferon-induced guanylate-binding protein 1 (GBP1), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="463"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="GB1/RHD3-type G" evidence="13">
        <location>
            <begin position="60"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="540" mass="60820" checksum="29C8D73EABF71119" modified="2018-12-05" version="1">SGASAFPYKPLITWEKNDNEPAAEERPGPVQIVRASESKCIFELDAAALEKILLQDHVKDLNVVVVSVAGAFRKGKSFLLDFMLRYMYNQHSDTWIGGDHEPLIGFKWRGGSERETTGIQVWSDVFVVDKPDGNKVAVLLVDTQGAFDSQSTLKDSATVFALSTMTSSVQVYNLSHNIQEDDLQHLQLFTEYGRLAMEETESKPFQSLMFLIRDWSSPYEYVYGLKGGQAFLQKRLQVKNCQHEELQNVRKHIDSCFSKISCFLLPHPGLKVATNPKFDGRLKDIEDDFKTLLAELMPVLLAPDQLVEKQIGGCKITCRDLVEYFKAYIKIYQGDELPHPKSMLQATAEANNLTAVAGAKDLYSKSMEKVCGGDRPYMAPADLESCHEAFSKDSVKFFRSIKKMGGEEFCQRYQDRLESELKEAYTNFSKHNEGKNIFYAARTPATLFVVMFFTYVLSGVTGFIGLSVIAALANMVLGVALLLLCIWAYVKYSGEFREAGILIDQLAEALWEQISGNLFLFKMFILRINHTHSPLGSSYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M972</accession>
    <name>A0A3B5M972_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arginine vasopressin-induced protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in MAP kinase activation, epithelial sodium channel (ENaC) down-regulation and cell cycling.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M972"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016309"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016515.1">
        <property type="protein sequence ID" value="ENSXCOP00000016309.1"/>
        <property type="gene ID" value="ENSXCOG00000012320.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000112219"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039579">
        <property type="entry name" value="AVPI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020282">
        <property type="entry name" value="Avpi1/C8orf4_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14350">
        <property type="entry name" value="ARGININE VASOPRESSIN-INDUCED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15063">
        <property type="entry name" value="TC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginine vasopressin-induced protein 1/transcriptional and immune response regulator" evidence="4">
        <location>
            <begin position="7"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="133" mass="15002" checksum="BCAC55297D55223F" modified="2018-12-05" version="1">MDTRSASASPSVVASPSSLWRLAERRNRKSGSGNIFSTVNLWQLQRLFRAAGDEDAEQRAQLVWGHRDEAELAKALVALRSQGHRRALRTNGRDAMGSHWLRAFNNLRATTTSSGLRRIGESNPERYLHRILH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9I3</accession>
    <name>A0A3B5M9I3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Paired domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9I3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017941"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018165.1">
        <property type="protein sequence ID" value="ENSXCOP00000017941.1"/>
        <property type="gene ID" value="ENSXCOG00000013518.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156759"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000080">
        <property type="entry name" value="paired box protein Pax-3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001523">
        <property type="entry name" value="Paired_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043565">
        <property type="entry name" value="PAX_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-6-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45636:SF26">
        <property type="entry name" value="PAIRED BOX PROTEIN PAX-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00292">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00351">
        <property type="entry name" value="PAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51057">
        <property type="entry name" value="PAIRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0563">Paired box</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017471615" description="Paired domain-containing protein" evidence="9">
        <location>
            <begin position="18"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Paired" evidence="10">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="143" mass="16022" checksum="F7C1D064C809AD85" modified="2018-12-05" version="1">MSVSFKLKSLLFVCSLAINLSNLKQVATPDVEKRIEEYKRENPGMFSWEIRDKLLKDGVCDRSTVPSGEASSVSSISRVLRARFGKKDDEDDCDKKDEDGEKKTKHSIDGILGDKCIQSDSICTFSQLFGWVEIEGDPNVGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9R2</accession>
    <name>A0A3B5M9R2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Caspase a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase C14A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9R2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016504"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016711.1">
        <property type="protein sequence ID" value="ENSXCOP00000016504.1"/>
        <property type="gene ID" value="ENSXCOG00000012455.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162428"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097169">
        <property type="term" value="C:AIM2 inflammasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072557">
        <property type="term" value="C:IPAF inflammasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072559">
        <property type="term" value="C:NLRP3 inflammasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050727">
        <property type="term" value="P:regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1470">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033139">
        <property type="entry name" value="Caspase_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016129">
        <property type="entry name" value="Caspase_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002398">
        <property type="entry name" value="Pept_C14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002138">
        <property type="entry name" value="Pept_C14_p10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47901">
        <property type="entry name" value="CASPASE RECRUITMENT DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47901:SF3">
        <property type="entry name" value="CASPASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038001">
        <property type="entry name" value="Caspase_ICE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00376">
        <property type="entry name" value="IL1BCENZYME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01122">
        <property type="entry name" value="CASPASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01121">
        <property type="entry name" value="CASPASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50207">
        <property type="entry name" value="CASPASE_P10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="CARD" evidence="10">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p20" evidence="9">
        <location>
            <begin position="131"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p10" evidence="8">
        <location>
            <begin position="276"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR038001-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="365" mass="42092" checksum="BDEF429D31F73F3D" modified="2018-12-05" version="1">MDNRKVKYRFVHKVSREVLRCLVDLLLEDQVLNGEEEDIICEGRYSRAGQARRLIDYVIRQGERASTRLIDHLQNIDPVLHKALGLSSDQPGLSGKRKKFQSTPSVPTVEDFLKTIRTNPEVYPVTQTSLKNRVALLITNFKFSDKSMNRRGAEKDKKNMEKLLSNLGYEVVKYTNLTGKAIDEALIYFTKHPKLKHTDSVFVVLMSHGKLGKILGVDWKEDKPDEFPINNIFKHLGSKSCPELRDKPKVIIIQACRGEHSAGYYVLQSEDHAADMLHHLILEKDFICFSSCVPDTVSYRQRDRRSLLIQNIVEAFSPKSYQEDICGLFEKIEMATHCYLFDEQRMSKITRNTLTKHFYPLTGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAX6</accession>
    <name>A0A3B5MAX6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kit ligand</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Mast cell growth factor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Stem cell factor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">c-Kit ligand</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Filopodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SCF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAX6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018653.1">
        <property type="protein sequence ID" value="ENSXCOP00000018421.1"/>
        <property type="gene ID" value="ENSXCOG00000013869.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018272"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030175">
        <property type="term" value="C:filopodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005173">
        <property type="term" value="F:stem cell factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003452">
        <property type="entry name" value="SCF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11574">
        <property type="entry name" value="KIT LIGAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11574:SF0">
        <property type="entry name" value="KIT LIGAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02404">
        <property type="entry name" value="SCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="20" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0339">Growth factor</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0964">Secreted</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00017304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00030364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00032898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00033123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="234" mass="26410" checksum="FAD034F0F9D97A4C" modified="2018-12-05" version="1">MGPLIRRNHEPDCIAKHLHVHAETQESGFLPTTCVLLGLFSSLHLCSGKFGTPITDDVSKLSVLKQNIPSDYEIPVGYIPREVAGTCWVVLNIYPLERSLEKLASMFGDMSSNRDDVNVFVTMLKSLRLTFDHEELVAMQVFQCHYREKSLLSGLYFDYIQDLLRAATQGTLSFSCKPQPCLNHPQTAGTCNYSLLAEGQEESRKYRWLKSSSLLLVLIPFTACIVLIVWQVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAY7</accession>
    <name>A0A3B5MAY7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL42</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL42 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAY7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018431"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018663.1">
        <property type="protein sequence ID" value="ENSXCOP00000018431.1"/>
        <property type="gene ID" value="ENSXCOG00000013875.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165713"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019346">
        <property type="entry name" value="Ribosomal_mL42"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13450:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13450">
        <property type="entry name" value="MITOCHONDRIAL 39S RIBOSOMAL PROTEIN L42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10210">
        <property type="entry name" value="MRP-S32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="133" mass="15069" checksum="6DF47D8389F424D0" modified="2018-12-05" version="1">PSMASGHLCKLHSCASRLLYGATLRNVQSKCCFYSYEVEIGVTSDGKTIVCYHPAVDVPYELTQPIERPDPLTNQAETHDQVLKAHLSKEVLDGKKGPTIEELSKMFFTTKHRFYPKGQYVSRRINKTPPKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MB73</accession>
    <name>A0A3B5MB73_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NAD(+) hydrolase SARM1</fullName>
            <ecNumber evidence="3">3.2.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">NADP(+) hydrolase SARM1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Sterile alpha and TIR motif-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>NAD(+) + H2O = ADP-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16301"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="EC" id="3.2.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:16302"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>NAD(+) = cyclic ADP-beta-D-ribose + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38611"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:73672"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:38612"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>NADP(+) + H2O = ADP-D-ribose 2'-phosphate + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19849"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58673"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:19850"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SARM1 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MB73"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016949"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017168.1">
        <property type="protein sequence ID" value="ENSXCOP00000016949.1"/>
        <property type="gene ID" value="ENSXCOG00000012777.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004155"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003953">
        <property type="term" value="F:NAD+ nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019677">
        <property type="term" value="P:NAD+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034128">
        <property type="term" value="P:negative regulation of MyD88-independent toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048679">
        <property type="term" value="P:regulation of axon regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:response to axon injury"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09501">
        <property type="entry name" value="SAM_SARM1-like_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09502">
        <property type="entry name" value="SAM_SARM1-like_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000043">
        <property type="entry name" value="Sterile alpha and TIR motif-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039184">
        <property type="entry name" value="SARM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22998:SF1">
        <property type="entry name" value="NAD(+) HYDROLASE SARM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22998">
        <property type="entry name" value="SARM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13676">
        <property type="entry name" value="TIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SAM" evidence="17">
        <location>
            <begin position="287"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="16">
        <location>
            <begin position="431"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="589" mass="66148" checksum="A2114B1DC78B6448" modified="2018-12-05" version="1">TVCITVTSMMSACKPLIGTVCLGRKHGLQLDLDYVARIGLGVILNLTRQQDDAQLARSVSGILEHMFKHTEETSVQLISNGALDALLFWCRGTDPTVLRHCAVALANCAMYGGHRCQRWMIEKQAAEWLFPLAFSKEDELIRFHACLAVTVLAANREIEKEVVRSGTLELVEPFIASLDPDDFARCLLDSADCMQGKTASDLQHLLPLLDGTRVEGKCIAAFYLCVETSIKSRQRNTKIFQEIGAVQSLKRIVMYSSNGTVSSLAKRALSMMGEEVPKRILSCVPNWKTCEVQTWLQQVGFSAFSDRFQELQVDGDLLLNITDEDLSSDLNMAAGLTRKRFLRDLRVLKTYADYSTCDPNNMADWLAEVDPRFRQYTYGLVQSGVDRNNVENLTDQQLQHDCHIDNGVHRAKILSTASRPLKPSHTDAQPPGPDVFISYRRTTGSQLASLLKVHLQVRGYSVFIDVEKLEAGKFEDKLIQSVQRARNFILVLSANALDKCMGDTAMKDWVHKEIATALSGKKNIVPVTDNFMWPDPMSLPEDMRAILNFNGIKWSHEYQEATIEKIIRFLKGRHDTTDAPDESKSQKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBC2</accession>
    <name>A0A3B5MBC2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G protein-coupled receptor kinase</fullName>
            <ecNumber evidence="10">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[G-protein-coupled receptor] + ATP = [G-protein-coupled receptor]-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12008"/>
            <dbReference type="Rhea" id="RHEA-COMP:11260"/>
            <dbReference type="Rhea" id="RHEA-COMP:11261"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBC2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020870"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021126.1">
        <property type="protein sequence ID" value="ENSXCOP00000020870.1"/>
        <property type="gene ID" value="ENSXCOG00000015628.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164321"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050254">
        <property type="term" value="F:rhodopsin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035469">
        <property type="term" value="P:determination of pancreatic left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001947">
        <property type="term" value="P:heart looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902017">
        <property type="term" value="P:regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000009">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000074">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000239">
        <property type="entry name" value="GPCR_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF26">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00717">
        <property type="entry name" value="GPCRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017412244" description="G protein-coupled receptor kinase" evidence="12">
        <location>
            <begin position="19"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="14">
        <location>
            <begin position="59"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="186"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="443"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="381"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="570" mass="65345" checksum="BC6DA33567B71213" modified="2018-12-05" version="1">FNVFPLVCVCVCVRAATGGGGKRKGRSKKWKEILRFPHISQCAELGKIIERDYASICDKQPIGRLLFRLYCETKPNLQRCIQLLDATCCLHQEVMDLCVSVYICDGVCLHVLMQSPQRVDIAEDFAEQCRENLELSPCKEIFSDCRKAVHEYLSGAPYSDYQNSMYFDRFLQWKMLERQPITKDTFRQYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGESMALNEKQILEKVNSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNMGTPGFDKDRVQFYAQSQTNMYMNLTMLFFHSVCGRLMTGHIRISDLGLAIKVPEGELIRGRVGTVGYMAPEVINNEKYAMSPDWWGLGCLVYEMTAGRSPFRARKERVKREEVERRVQEEEEEYNDKFTEDAKAICRMLLTKDPKQRLGCNADGAAGVKAHSFFKNINFKRMEAGIVDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDQTDNDFYSKFATGCVPIPWQNEMIETECFRDLNVFGPQGTRPPDLDWNQPPEPPRRSLLDRIFRRHVSGCERTYGGGSRRARSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBG6</accession>
    <name>A0A3B5MBG6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SEA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBG6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020937"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021194.1">
        <property type="protein sequence ID" value="ENSXCOP00000020937.1"/>
        <property type="gene ID" value="ENSXCOG00000015675.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000172773"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="55716" checksum="E48D7EE0F7A96873" modified="2018-12-05" version="1">PVNTISSILNATSSPVSPTSPAANVTSPPLAATSEPISPTLPAANITSHPLTATSEPISPTSPAANITSPPLTATSEPISPTSPAANITSHQLTATSEPMSPTSPAANITSHPLIATSEPVSPTSPAANVTSPPLTATSEPISPTSPAANITSPPLTATSEPISPTSPAANITSHPLTATSEPISPTSPAANITSHPLTATSEPVSPTSPAANVTSPPLTATSEPISPTSPAANITSPPLTATSEPISPTSPAANITSHPLTATSEPISPTSPAANITSPPLTATSEPISPTSPAANITSHPLTATSEPISPTSPAANITSHPLTATSEPISPTSPAANITSPPLTATSELISTTSQAANVSLPSLTATSVPVSPTSPAANVTSTPFTATSVPVSPTPLTSRSSPTITMNSTTVNITSTLPIPTSLPGNFLSIIDLYVGFNKRSMTDLPHVYLLALKLQTIHFYSLHCSKTTPIMQILTSSMSSIHILKEGSIVVDSELIFNNITEIPNASVIAESLKNASSTPGFGLKVNISTIAAAGKVYLLPLKNVDSCKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5ME21</accession>
    <name>A0A3B5ME21_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 25">Receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="3 25">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="25">Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="3 25"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5ME21"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021790"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022054.1">
        <property type="protein sequence ID" value="ENSXCOP00000021790.1"/>
        <property type="gene ID" value="ENSXCOG00000016121.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155450"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009925">
        <property type="term" value="C:basal plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048408">
        <property type="term" value="F:epidermal growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005006">
        <property type="term" value="F:epidermal growth factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010647">
        <property type="term" value="P:positive regulation of cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050679">
        <property type="term" value="P:positive regulation of epithelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023056">
        <property type="term" value="P:positive regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05108">
        <property type="entry name" value="PTKc_EGFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12087">
        <property type="entry name" value="TM_EGFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.20.20:FF:000013">
        <property type="entry name" value="Erb-b2 receptor tyrosine kinase 3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000001">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000002">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000044">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.20.20:FF:000006">
        <property type="entry name" value="Receptor protein-tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002828">
        <property type="entry name" value="Receptor tyrosine-protein kinase erbB-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.20.20">
        <property type="entry name" value="Receptor L-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044912">
        <property type="entry name" value="Egfr_JX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006211">
        <property type="entry name" value="Furin-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032778">
        <property type="entry name" value="GF_recep_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000494">
        <property type="entry name" value="Rcpt_L-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036941">
        <property type="entry name" value="Rcpt_L-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049328">
        <property type="entry name" value="TM_ErbB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016245">
        <property type="entry name" value="Tyr_kinase_EGF/ERB/XmrK_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF91">
        <property type="entry name" value="EPIDERMAL GROWTH FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00757">
        <property type="entry name" value="Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14843">
        <property type="entry name" value="GF_recep_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01030">
        <property type="entry name" value="Recep_L_domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21314">
        <property type="entry name" value="TM_ErbB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000619">
        <property type="entry name" value="TyrPK_EGF-R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0010">Activator</keyword>
    <keyword evidence="12 25 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="22" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 25" id="KW-0418">Kinase</keyword>
    <keyword evidence="16 25 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 25 28" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21 25" id="KW-0675">Receptor</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="20" id="KW-0804">Transcription</keyword>
    <keyword evidence="15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 25" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 29" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 29" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="17 25" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="652"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="30">
        <location>
            <begin position="719"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="26">
        <location>
            <position position="844"/>
        </location>
    </feature>
    <feature type="binding site" evidence="27">
        <location>
            <begin position="725"/>
            <end position="733"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="27 28">
        <location>
            <position position="752"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRNR" id="PIRNR000619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR000619-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR000619-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1206" mass="134125" checksum="B8B635D7F463F2B1" modified="2018-12-05" version="1">FFKIPFEGFFFSLLLGCSQNQIIFFFKIFFNFFPVCQGFSNRVNLMDNKEDHYLNMVKTYSNCTIVLENLEITHVDENRDLSFLKSIEEVSGYVLIALNTVSRIPLENLRIIRGHSLYEEKFALSVLANIDKNTGQGTTELLLTSLTEILRGGVKFLNNKLCNVETIQWFDIVNADTKPSMELQVAGNNPFCKCNKCHSSCYNGSCWAPGAENCQTLTKLNCAQQCSKRCKGPSPSDCCNEHCAAGCTGPTAADCLACRDFQDDGVCKDSCPGLMRYDPNLHQLVPNPQGKYNFGATCVKNCPHNYVVTDHGACVRTCSGNTYEVEEGGIRKCAKCNGLCPKVCNGLGYGDLTYILSVNATNIGSFKNCTKITGDITILRTSIHGDPYTKTPKMEPAQLDVFKTVKEITGHLWIQDWPKSMNSLSPFENLEIIRGRTKRGSRSLVVTLLDIDYLGFRSLKEISDGDVVIIKNHNLCYMDDSYWKRFFKSGGQTATVNENANATICVLRNKTCDRKCTADGCWGLGPDMCFACRDYSRGGSCVDFCNILEGEPREAVMNKTCMECDPECLRMNGTATCQAPGPGNCTKCANFQDGLYCVPRCPQGVPGEGDTLVWKYVDEKRVCQLCHKNCTKGCVGPGPEGCQGERPSGLSIIAAGVVGGLLAVLIAGLSVFVLLRRRHIKRKRTMRRLLQERALVEPLTPSGQAPNQALLRILKEPEFKKIKVLGSGAFGTVYKGLWVPEGEDVKIPVAIKVLREATSPKANQEILDEAYVMASVEHPHVCRLLGICLTSTVQLITQLMPYGCLLDYVKEKKDNIGSQHLLNWCVQIAKGMNYLEERHLVHRDLAARNVLVKTPHHVKITDFGLAKLLNADEKEYHADGGKVPIKWMALESILNRTYTHQSDVWSYGVTVWELMTFGTKPYDGIPASEIAGILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPHFRELIAEFTKMARDPPRYLVIQGDDRMHLPSPTDTKFYRSLISGGDLDYGVDAEEYLVPQHSFFSSPSTSYTPLLHSTSINSSIGTSKTRNGLMNGVPSRDGSTVLRYMPDPTDKFLDQAFLPAPEYMNQNGISNVINYVYQHSGPPRTLLPTISSDDTESEYLNYFKNGANGPEYLNELPSSTALPLNSNNTAIGGQNYRPQNSMDNPDYQQDFTPTFKTHTNGHIPAAENVEYLGPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFZ2</accession>
    <name>A0A3B5MFZ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027858548.1">
        <property type="nucleotide sequence ID" value="XM_028002747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MFZ2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018752.1">
        <property type="protein sequence ID" value="ENSXCOP00000018519.1"/>
        <property type="gene ID" value="ENSXCOG00000013939.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114135443"/>
    <dbReference type="KEGG" id="xco:114135443"/>
    <dbReference type="GeneTree" id="ENSGT00940000161337"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00637">
        <property type="entry name" value="7tm_classA_rhodopsin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000096">
        <property type="entry name" value="Odorant receptor 131-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF998">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="6">
        <location>
            <begin position="38"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="305" mass="35720" checksum="D139840B0A70DBCF" modified="2018-12-05" version="1">MNLSAANVTVVVQYGDSFSKALIKNLIVVVLSIFINYINAGLIYTFSKHQIFNTNPRYILFVHLVINDMIQVTLTVALFITSYIFFRIPMTVCCFFILIAIFTIENTPLNLACMAVECYIAICIPLRHSQICTVKKTRLLILLVWATSLVSVLPDLFMTLATESQDYFKLRVFCVRERVFPNPRIIERRNITYIVYLVLVWCIIFFIYFRIMFTARTASKDAKKARDTILLHGFQLLLCMATYAEHLVKDMVWQWFRDYYLDALFACYLLFQIIPRFISSIIYGVRDKTFRKHLKGYLLYKISPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHA4</accession>
    <name>A0A3B5MHA4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="8">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="8">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.</text>
    </comment>
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    <dbReference type="AlphaFoldDB" id="A0A3B5MHA4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020496"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020746.1">
        <property type="protein sequence ID" value="ENSXCOP00000020496.1"/>
        <property type="gene ID" value="ENSXCOG00000015361.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:hydrolase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:RNA binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:RNA helicase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA helicase"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
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        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
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        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
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        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="101"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="273"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="545" mass="61309" checksum="8C92872E41FCF75F" modified="2018-12-05" version="1">EPAAAGPTEPERNQQNPAEEEEAEETPLKKQKKKKKRKLADGAAGSVSTRKQTGFTGFTGFTGAFEDTSFASLTGLVGDHILRAIKEMGFDHMTEIQHKTIRPLLEGRDILAAAKTGSGKTLAFLIPSIELINKLKFMPRNGTGVVILSPTRELAMQTYGVMKELMAHDVHTFGLIMGGSNRSAEAQRLANGVNILVATPGRLLDHLQVRRWNCRRVLHQRLGSAFGCLPKRQTMLFSATQTRRVEDLARISLKKEPLYVGVDDHKDSATVDGLEQGYVVCPSEKRFLLLFTFLKKNRRKKLMVFFSSCMSVKFHYELLNYIDLPVMAIHGKQKQTKRTTTFFQFCNADSGILLCTDVAARGLDIPEVDWIVQYDPPDDPKEYIHRVGRTARGIDGRGHALLILRPQELGFLRFLKQAKVPLSEFEFSWSKISDIQSQLEKLIEKNYYLHKSAQEAYKSYVRAYDSHSLKQIYSVGTLNLPMVALSFGFTVPPYVDLNVHSSKGLKLQKRGGGGGFGYQKAKNVRKSRIFKHVSRGPSDRRQFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHK9</accession>
    <name>A0A3B5MHK9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019059"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019296.1">
        <property type="protein sequence ID" value="ENSXCOP00000019059.1"/>
        <property type="gene ID" value="ENSXCOG00000014324.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166425"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051718">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.50">
        <property type="entry name" value="PWWP, helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025766">
        <property type="entry name" value="ADD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018117">
        <property type="entry name" value="C5_DNA_meth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040552">
        <property type="entry name" value="DNMT3_ADD_GATA1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049554">
        <property type="entry name" value="DNMT3_ADD_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23068:SF53">
        <property type="entry name" value="DNA (CYTOSINE-5-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23068">
        <property type="entry name" value="DNA CYTOSINE-5- -METHYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17980">
        <property type="entry name" value="ADD_DNMT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21255">
        <property type="entry name" value="DNMT3_ADD_GATA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00293">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51533">
        <property type="entry name" value="ADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00094">
        <property type="entry name" value="C5_MTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="13">
        <location>
            <begin position="15"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="PWWP" evidence="14">
        <location>
            <begin position="836"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="15">
        <location>
            <begin position="1003"/>
            <end position="1135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="803"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="958"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="647"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="816"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="960"/>
            <end position="974"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1378" mass="155136" checksum="B82CD66A09256EB9" modified="2018-12-05" version="1">MAGSPVVTPNSIRDKSSRFYVVSWINRLLKTDFKNVQEMGTGACQCQMMDCTFPGSIDMAKVKFDAQSKEDFRHNFCLLQDAFNLKGVKKTIPVDGLIEGDFKTCYEFMKWLKLFYTENTKNKESTPVKATICNSEEITPVSPSSENSDMETNGTERTTKTFLYTEKWKEEYGWADPSPLGEQHTYCSICHTNLASYRRGAVELQRHMDTNKHQKRAKDFQMCQNETEVGERFPCSDAAVRFIFNRCYTGSAKGEQVSRHFARCKLGLKYPKDLVAVSNRTPYCVYVYEALAAGNDDTVAVVLLGFFDSESSGHRICLLDAVESADGAGDQTAAAVVQALKRLELSTDNLAVCYPGDGEASEQICSHLRELNPNIIALGGLYSIADAAYHAGVQELPSEIQELLVDLYAHYSSCSTKNDNLKALFDSDITKNSKPFYLSISCLRFHQLITKLTEIWSDLILYFSSCPEDEEKGKSLCSQLKNPKVKAMFMLLDYALKPLETFQKQIQESSWAKMPLILEEASMLLSTYTSRFLQPNAATRYLNEHNAQILKNKKHHLSGPDLNLGGKDMEDFLKTEEAAEVLPQLQEELLLFYVAATCCIAEELPLSDTVLKNIAQLLNPESSLKVTDVAMEELGRKLGICSSPEEVRQLKQEFREYQQAEEERSNQEVNGDTLENHWARVLEGTKPTSVFRKLVLTLLSFPCPPLDAQHVLSQALDNNDVAPCSESDTVTESESEVIPDRVLTNGSKKDLHLYTAECVLSQGVWRGGHGWESSLRQKPQARKVFQAGVGTWSKPIGLIKGHKSGADSIDEEVGISPSSKNTPSKGQYQDGKGFSVGELVWGKLTGMSMWPGLVMPWKSKHAPPGMRRVEWFGDGMFSEVSTERLKPFSLFTLCFCKNSFASLPVYKDAIYQICELAAERSGKSFSKAEGDKERELKMMVDWASEGFLPTGAKGFAPLESSSNQDPSDSPSSDYQPPPKRKYVYKNRAAAPNTPYNRESLKEKVKEKGKTIEDFCLSCASSEIDVQHPLFKGGLCLRCKENFTETLYRYDEDGYQSYCTVCCAGLEVILCGNASCCRCFCKDCLDILVAEGTFNKLKDIDPWSCFMCEPSQCDGNLKLRPDWSVKIQDLFTNNSAMEFEPHRIYPSIPADQRKPLKVLSLFDGIATANNECFDIATGYLVLKDLGFKIKRYIASEICDDSIVVGMIKHEGKIEYVNDVRTITRKHLAEWGPFDLLIGGSPCNDLSMVNPLRKGLFEGTGRLFFEFYRILTLLKPKEDDNRPFFWLFENVVLMSAHDKTDICRFLECNPVLVDAVKVSPAHRARYFWGNIPGMSRYVLIQSCSFLTIVEPHFLLKQSHSCCIFTYIFVFFKDFLLCMQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHP0</accession>
    <name>A0A3B5MHP0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Tectonic family member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TCTN2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Part of the tectonic-like complex (also named B9 complex).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tectonic family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHP0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023135"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023413.1">
        <property type="protein sequence ID" value="ENSXCOP00000023135.1"/>
        <property type="gene ID" value="ENSXCOG00000017260.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00570000079101"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036038">
        <property type="term" value="C:MKS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904491">
        <property type="term" value="P:protein localization to ciliary transition zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040354">
        <property type="entry name" value="TCTN1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011677">
        <property type="entry name" value="TCTN1-3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057724">
        <property type="entry name" value="TCTN1-3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14611">
        <property type="entry name" value="TECTONIC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14611:SF6">
        <property type="entry name" value="TECTONIC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25752">
        <property type="entry name" value="DUF1619_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07773">
        <property type="entry name" value="TCTN_DUF1619"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="524"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Tectonic-1-3 N-terminal" evidence="8">
        <location>
            <begin position="104"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Tectonic-1-3" evidence="7">
        <location>
            <begin position="311"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="558" mass="61475" checksum="33B82F59B0188DD7" modified="2018-12-05" version="1">KVSAFLLGNTSRISLNLKTVSVSITTGRKKKKTKHPTKHLLLSAIRVQLTLNQSLHLCGENETSPDCCPKPQCVLETLQVTACLGSRPQASLLIQATINSRLFISDNNTVFPNQVYQPLGVCPCDLTSRACDVRCCCDQDCSNEDLKIFASHCLLGPFGGQVSPAPDFQCSVHSAENSPDWFEFLCVTSPPENNPYLGLFYQGDTTPGPSFQEPVRSAPMPFTPYIQGSPVVTSDDKYFAIPQMVLGRCSGAPVAFLQNFEVECITRFNSCPTGPPLKTLQEDLSVDVLNGQGGGSYLCYSHGGLYQFHMAGYQVGRPVIAGILVNNTELIQRTPINVWKPVGDGLCSTDSMKPVVFGENSTSGCLLYFAFTYICLVVLCQIDVLTNDLLHKSNAVMTPNSSSVMNNNSGVCSDIPFHQHISVWSLTTSMVDGIPQREIQALEISYGMSTWALDCRGGSISVCEDPEKRQLFPIISSVTFFDIDTGPAKTRFQINFTEYDCDRNDVCWPELAFPFTRHYTGEPYFLSLAKGMVLVFMFVTAAILGTPWRQIRQAWRNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIV2</accession>
    <name>A0A3B5MIV2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone-binding protein RBBP4-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIV2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023682"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023965.1">
        <property type="protein sequence ID" value="ENSXCOP00000023682.1"/>
        <property type="gene ID" value="ENSXCOG00000017690.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154748"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000021">
        <property type="entry name" value="histone-binding protein RBBP4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 11" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="12">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="138"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="188"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="234"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="278"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="335"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="382" mass="42648" checksum="0657327940F5E9C6" modified="2018-12-05" version="1">MTHALEWPSLTVQWLPDVNRPEGKDYTVHRLVLGTHTSDEQNHLVIASVQVPNDDAQFDASHYDSEKGEFGGFGSVSGKIEIEIKINHEGEVNRARYMPQNPCIIATKTPTSDVLVFDYTKHPSKPDPSGECSPDLRLKGHQKEGYGLSWNPNLSGNLLSASDDHTICLWDIGTGPKEGKIVDAKTIFTGHTAVVEDVSWHLLHESLFGSVADDQKLMIWDTRSNNTSKASHSVDAHTAEVNCLSFNPYSEFILATGSADKTVALWDLRNLKLKLHSFESHKDEIFQVQWSPHNETILASSGTDRRLNVWDLSKIGEEQSSEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWVICSVSEDNIMQVWQMVRTVLIQSLSRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MK65</accession>
    <name>A0A3B5MK65_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MK65"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023970"/>
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        <property type="protein sequence ID" value="ENSXCOP00000023970.1"/>
        <property type="gene ID" value="ENSXCOG00000017900.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR051360">
        <property type="entry name" value="Neuronal_Pentraxin_Related"/>
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        <property type="entry name" value="NEURONAL PENTRAXIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277">
        <property type="entry name" value="PENTRAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="215" mass="24056" checksum="B266166F97412D16" modified="2018-12-05" version="1">MHQATPNAAALHKLLLNKSFGACRDGKAPNSDPVSTSLFCRQTLVRFLTTARTASPRTHQRRASQRGQPLRAAVPVLTGPGGWRTWKRSWRGRWSCDAGLSFPEGYRLSFPARTNYMFALMRHAVPKLRAFTACLWLRPAAAGIGTPLSYAVAEQPNELVLLQDRSAPAELLIDDKVRNPHRAEKAKHLTEAGIGWNDNLEEKYHGLTFVRDFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKN9</accession>
    <name>A0A3B5MKN9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Zinc finger, matrin-type 4a</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKN9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020089"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020336.1">
        <property type="protein sequence ID" value="ENSXCOP00000020089.1"/>
        <property type="gene ID" value="ENSXCOG00000015061.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051868">
        <property type="entry name" value="ZN346_ZMAT4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46144:SF3">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46144">
        <property type="entry name" value="ZINC FINGER PROTEIN 385B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="124" mass="13971" checksum="7603313D6EAFC381" modified="2018-12-05" version="1">MKSTEVDQGLFTDSYCKVCSAQLISESQRVAHYEVKMASKITSSQSVTLHFWVHGSDESDVDKNKCCTLCNMSFTSAVVAQSHYQGKIHAKRLKLLLGEQPAITTKGIFYDWIKKCHDEEAIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKX2</accession>
    <name>A0A3B5MKX2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Katanin p60 ATPase-containing subunit A1</fullName>
            <shortName evidence="8">Katanin p60 subunit A1</shortName>
            <ecNumber evidence="8">5.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">p60 katanin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">KATNA1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers.</text>
            <dbReference type="EC" id="5.6.1.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="8">ATPase activity is stimulated by microtubules, which promote homooligomerization. ATP-dependent microtubule severing is stimulated by interaction with KATNB1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Can homooligomerize into hexameric rings, which may be promoted by interaction with microtubules. Interacts with KATNB1, which may serve as a targeting subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
            <location evidence="1 8">Cytoskeleton</location>
            <location evidence="1 8">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
            <location evidence="8">Cytoskeleton</location>
            <location evidence="8">Microtubule organizing center</location>
            <location evidence="8">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
            <location evidence="8">Cytoskeleton</location>
            <location evidence="8">Spindle pole</location>
        </subcellularLocation>
        <text evidence="8">Predominantly cytoplasmic. Also localized to the interphase centrosome and the mitotic spindle poles. Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with KATNB1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the AAA ATPase family. Katanin p60 subunit A1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.1" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKX2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025034"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025339.1">
        <property type="protein sequence ID" value="ENSXCOP00000025034.1"/>
        <property type="gene ID" value="ENSXCOG00000018687.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156630"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008568">
        <property type="term" value="F:microtubule severing ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051013">
        <property type="term" value="P:microtubule severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21748">
        <property type="entry name" value="Kp60-NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19522">
        <property type="entry name" value="RecA-like_KTNA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000025">
        <property type="entry name" value="Katanin p60 ATPase-containing subunit A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000003">
        <property type="entry name" value="Katanin p60 ATPase-containing subunit A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000159">
        <property type="entry name" value="Katanin p60 ATPase-containing subunit A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03023">
        <property type="entry name" value="Katanin_p60_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028596">
        <property type="entry name" value="KATNA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048611">
        <property type="entry name" value="KATNA1_MIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048612">
        <property type="entry name" value="KTNA1_AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF65">
        <property type="entry name" value="KATANIN P60 ATPASE-CONTAINING SUBUNIT A-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21126">
        <property type="entry name" value="KATNA1_MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="227"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="112"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="140"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="235"/>
            <end position="242"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="477" mass="53833" checksum="BB52EB4103A1F6C3" modified="2018-12-05" version="1">MNLAEICDNAKKGREYALLGNYDSSVVYYQGVIQQIHKHSQSLRDPALKVKWQQLRQELTEEYEQVKSIMGTLESFKSEKPVDILVPLERPEDPAVWPPPIPVEHRNPVVVKRPNSAIKQHRKDSPGMQHRGGRAQANPKAERQANRAAKAKDDKKVDSLAEGEQKKFDGTGYDSDLVDSLERDIISRNPNVHWDDIADLEDAKKLLREAVVLPMWMPDFFKGIRRPWKGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYAPTTIFIDEIDSICGRRGTSDEHEASRRVKSELLIQMDGVGGALENDDPSKMVMVLAATNFPWDIDEALRRRLEKRIYIPLPTAVGRVELLKINLREVELAPDVNLDLIAEKIEGYSGADITNVCRDASMMAMRRRIQGLSPEEIRALSKDELQMPVTMEDFTMTLKKISKSVSAADLEKYESWMAEFGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKX8</accession>
    <name>A0A3B5MKX8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKX8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021796"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022060.1">
        <property type="protein sequence ID" value="ENSXCOP00000021796.1"/>
        <property type="gene ID" value="ENSXCOG00000016291.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271858"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000366">
        <property type="entry name" value="GTPase, IMAP family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF186">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="212" mass="23696" checksum="1FC2076E71E62C34" modified="2018-12-05" version="1">LRIVLLGKTGVGKSAAGNTMLGGRFFHSCLSFSSMTSVCEKKINDFDGLNLAVIDTPGLFDTNKSHKEIMEEIAKGICLAAPGPHVFLIVLQPTRFTTEEERSLKIIQKIFGNEAATYTMILFTHGDELKRENIAIGEMLLKHRPLRHFISQCAFFQTFEDKYHVFDNTVEDPGQVRGLVQKINRLVKGNGGSFYTNEMFKEAVQKMGCSTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPI1</accession>
    <name>A0A3B5MPI1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Phosphoinositide-3-kinase interacting protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MPI1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023081"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023358.1">
        <property type="protein sequence ID" value="ENSXCOP00000023081.1"/>
        <property type="gene ID" value="ENSXCOG00000017247.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017774"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051898">
        <property type="term" value="P:negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00108">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.20.10:FF:000001">
        <property type="entry name" value="Urokinase-type plasminogen activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.20.10">
        <property type="entry name" value="Plasminogen Kringle 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000001">
        <property type="entry name" value="Kringle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018056">
        <property type="entry name" value="Kringle_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038178">
        <property type="entry name" value="Kringle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050759">
        <property type="entry name" value="Serine_protease_kringle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24261:SF16">
        <property type="entry name" value="PHOSPHOINOSITIDE-3-KINASE-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24261">
        <property type="entry name" value="PLASMINOGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00051">
        <property type="entry name" value="Kringle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00018">
        <property type="entry name" value="KRINGLE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00130">
        <property type="entry name" value="KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00021">
        <property type="entry name" value="KRINGLE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50070">
        <property type="entry name" value="KRINGLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 4" id="KW-0420">Kringle</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017181216" evidence="6">
        <location>
            <begin position="22"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="7">
        <location>
            <begin position="17"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="39"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="243" mass="26894" checksum="1B1393203A6E7043" modified="2018-12-05" version="1">MFFSLHIVFLSAAIVEDCVRSRGLYYRGEQQSSSTGLTCLNWINTTRDYDVEMHPDSQTGAGDHNYCRNPDASEGPWCYIAAPDGTIQRQFCAIEMCKGKPTEITSSTQRFQAATPSKQRDVAAVQPVMGVSQRVRTGPKKKKDLGTTGMVLGSLMMAIIIILGVGITLGYFYKRGQNLKKQHEQRRYEREMQRISLPLSAFSNPTCELIDENTIVITAEHETTPVREGGDPLMGQHAGTPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSY4</accession>
    <name>A0A3B5MSY4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSY4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024614.1">
        <property type="protein sequence ID" value="ENSXCOP00000024321.1"/>
        <property type="gene ID" value="ENSXCOG00000018165.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017328"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045639">
        <property type="term" value="P:positive regulation of myeloid cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040117">
        <property type="entry name" value="GCSF/MGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10511">
        <property type="entry name" value="GRANULOCYTE COLONY-STIMULATING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10511:SF2">
        <property type="entry name" value="GRANULOCYTE COLONY-STIMULATING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="212" mass="23347" checksum="C5A035889FB255E1" modified="2018-12-05" version="1">INTLIVPICFLPSVLKMLQYFLFASLVRSAPAGLPSNLPPEFRETALKAMMLGEKILKDISAAHAAAVKDFSLDSSTSNLQSMAVMMEIPLSPVIQAVSPEFPLETLVSHMLAGVQMYQNLLEVLSSKVSGLDDLRADLRDLLNHINKLKEVAQLTVEVSDQNPSTNLASHLQNSHNVPVAFRLTFTRLRSFCHDLNRTFKHLSKLLAAGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MTG1</accession>
    <name>A0A3B5MTG1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">acetyl-CoA carboxylase</fullName>
            <ecNumber evidence="3">6.4.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="6.4.1.2" evidence="3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022779"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023055.1">
        <property type="protein sequence ID" value="ENSXCOP00000022779.1"/>
        <property type="gene ID" value="ENSXCOG00000017018.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155049"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.460.10:FF:000001">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000005">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000010">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.460.10">
        <property type="entry name" value="Biotin dependent carboxylase carboxyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1770.10">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049076">
        <property type="entry name" value="ACCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049074">
        <property type="entry name" value="ACCA_BT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013537">
        <property type="entry name" value="AcCoA_COase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728:SF1">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08326">
        <property type="entry name" value="ACC_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21385">
        <property type="entry name" value="ACCA_BT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0092">Biotin</keyword>
    <keyword evidence="10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="17">
        <location>
            <begin position="68"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="16">
        <location>
            <begin position="224"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="15">
        <location>
            <begin position="691"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="18">
        <location>
            <begin position="1507"/>
            <end position="1837"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="19">
        <location>
            <begin position="1841"/>
            <end position="2152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="2262" mass="255849" checksum="7ABDA00F6C805279" modified="2018-12-05" version="1">RLKFFLGASEDNSSDDEVLVTKPPSGAPSMSGPHLVKKGREHRKMDLQRDFTVASPAEFVTRFGGNRVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERIIRFVVMVTPEDLKANAYIKMADHYVPVPGGTNNNNYANVELIVDVAKRIPVQAVWAGWGHASENPKLPELLNKSGITFLPSSKAMWALGDKVASSIVAQSADIPTLPWSGSLRVEWTEEYQRRGYVISVPPEVYKEGCVHDVDDGLAEAEKIGYPVVIKASEGGGGKGIRKVENSDEFSSFFRQVQTEVPGSPIFIMQLAQHARHLEVQILADEYGNAISLFGRDCSIQRRHQKIIEEAPATIAPASTLEQMERHAVRMAKMVGYVSAGTVEYLYSEDGSFHFLELNPRLQVEHPCTEMIGDVNLPAAQLQIAMGIPLHRIKDIRVLYGESPWGDSIINFETPDCIPIPRGHVIAARITSENPDEGFKPSSGTVQELNFRSSKNVWGYFSVGAAGGLHEFADSQFGHCFSWGENREEAINMVVAMKELSIRGDFRTTVEYLIKLLETESFRNNDIDTGWLDHLIAEKVQAERPDTMLGIVCGALHVADFSFRKSMSDFLHSLEGQVLAADSLLNSVNIDLIYEGVKYCLKVARQSQTTYVIIMNGSDIEIDVHRLNDGGLLLCYDGNSHTTYMKEEVDYRVTVGNKTCVFEKERDPTVLRSPSAGKLLQFTVEDGDHIFAGEPYAEIEVMKMVMTLTVPQSGCIHFVKRPGAVLEPGCVTARMELDDPSSIHKVELNTATLPPQQPLPMVGEKLHQVFHNVLENLVRVMDGYCLEEPYFSTKLKEWVATLMKTLRDPSLPLLELQEIMTSVASRIPPTVEKDIRKVMAQYASNITSVLCQFPSQRFQIANILDSHAATLQRKADREVFFMNTQSIVQLVQYRSGTRGYMKSVVLDLLKRYLQVEMQFQQHYDKCVINLREKHKPDMSPVLDYIFSHAQVFKKNILVTMLIDQLCGRDATLADELMAILNEFTQLSKMENSKVALRARQVLIASHMPSYELRHNQVESIFLSAIDMYGHQFCPENLKKLILSETSIFDVLPNFFYHTNQVVCMAALEVYVRRAYIAYELNSIQHHQLLDGTCAVDFQFMLPSSHPNGSSPTLNFRLPVAAGGLGQFKVQRQSSELLMDGAFSPPCQRMGAMVAFQCFDDFKNFDEVLSSFAEQLVEGASFLESCSSFYEEENMKNNRENPIHIINVSIRTADTEDDDALVKAFTAFAQSKKAILFEYGIRRMTFLITQKREFPKFFTFRARDEFQEDRIYRNLEPALAFQLELNRMRNFDLTAVPCANKKMHLYLGAARVQEGAEVTDYRFFIRAIMRHSDLITKEASFEYLQNEGERLLLEAMDELEVAFNYTSVRTDCNHIFLNFVPTVIMDPSKQIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGNAIPIRLFLTNESGYYLDISLYKEVTEPSSRQIMFQSYGDKQGPLHGMLINTPYVTKDLLQAKRFQAQSLGTTYVYDVPEMFRQALFKLWVPGDKCPKDVLICTELVLDPEGRLVQMNRQPADNDVGMVAFRMKMKTPEYPEGRDIIVICNDITHMIGSFGPQEDELFLKASELARAEGIPRIYIAANSGARIGLAEEVKHMFQVAWIDPADPYKGFKYLYLSPQDYTRISSTNSVHCQHVEEGGESYIITDIIGKDEGLGVENLRGSGTIAGESSQAYEEIITISMQVTCRAIGIGAYLVRLGQRVIQVENSHIILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHTTVPDDFEGVFTILKWLSYMPKNNHSPVPVRATTDPVDREIEFVPTKAPYDPRWMLAGRPHPSRGVWQSGFFDHGSFMEIMGSWAQAVVVGRALGGIPLGVIAVETRTVELTIPADPANLDSESKVLQQAGQVWFPDSAFKTAQAICDFNREHLPLMVFANWRGFSGGMKMYDQVLKFGAYIVDALHSFHQPVLVYIPPYAELRGGSWVVIDPTINLLCMELYADRESGGVLEPEGTVEIKFRRKDLLKTMRRLDSVYASLVENLSPELSDKEIKELEKKLKAREEFLLPIYHQVAVQFVDLHDTPGRMQEKGVITDILDWKQARTFFYWRLRRLLLEQVVKCEIMEANKDLSDGHMQSMLRRWFVETEGTVKAYLWDNNQAVVEWLEKHLSTEDGTRSVIRENIKYLKRENALKHIRLVQANPDIAMDCIIHMSQNISPSQRASLSHLLATMDTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUQ6</accession>
    <name>A0A3B5MUQ6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Myosin VIIA</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MUQ6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024992"/>
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        <property type="protein sequence ID" value="ENSXCOP00000024992.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:myosin complex"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:actin binding"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
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        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
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        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
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        <property type="entry name" value="Kinesin_motor_dom_sf"/>
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        <property type="entry name" value="Myosin_head_motor_dom-like"/>
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        <property type="entry name" value="Myosin_VII_N"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="MYOSIN VIIA"/>
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    </dbReference>
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        <property type="entry name" value="Myosin_VII_N"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
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    <dbReference type="SMART" id="SM00139">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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        <property type="match status" value="4"/>
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    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="6 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="12">
        <location>
            <begin position="65"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="MyTH4" evidence="11">
        <location>
            <begin position="1019"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="9">
        <location>
            <begin position="618"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="905"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
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        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="158"/>
            <end position="165"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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            <dbReference type="ARBA" id="ARBA00023175"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
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        </source>
    </evidence>
    <sequence length="1193" mass="138162" checksum="F6DE8BE112E8E6C4" modified="2025-10-08" version="2">MFFLMQGDYVWLDLKTGREFVVPVGAVVKLCDSGQIQVLDDEGREHWIFPQNATNIKPMHPTSIHGVEDMIRLGDLNEAGILRNLLIRYNEHVIYTYTGSILVAVNPYQLLPIYTPDQIRLYTNRKIGEMPPHIFAIADNCYFNMQRNNKDQCCIISGESGAGKTESTKLILQFLAAISGQHSWIEQQVLEATPILEAFGNAKTIRNDNSSRFGKYIDIHFNKRGAIEGAKIEQYLLEKSRVCRQAADERNYHIFYCMLKGMGPEMKAKLGLGLARDYCYLTAGNCTECDGRDDLADYSSILSAMKVLMFSETENVEISKLLAAILHMGNLRFEARTYDNLDACVVVRSPDLVTAASLMEVEPKDVMVCLTTRTLITRGESVTTPLSVEQGLDVRDAFVKGIYGRLFVWIVDKINAAIYRPPSCESSVIRRSIGLLDIFGFENFIVNSFEQLCINFANENLQQFFVRHVFKLEQEEYNLEDINWQHIEFTDNQEALDMIANKPMNIISLIDEESKFPKGTDATMLYKLNSQHKLNTNYIPPKNSYETQFGIQHFAGVVHYESRGFLEKNRDSLHTDIIQLVHSSKNKFIKQIFQADVAMGVETRKRSPTLSSQFKRSLEMLMRTLSVCQPFFVRCIKPNEFKKPLLFDRELCIRQLRYSGMMETIRIRRAGYPIRYSFAEFVDRYRVLMPGIKPAHIQDDLKGTCQQIVSARLGKQEDWQIGKTKIFLKDHHDMQLEIERDKAITDKVILIQKSVRGLKARSNFRRLRSAVVCIQKVWRGYRSRKNYQIMKSGFIRLQAVCRSRKYYRSYRITRCRITLIQARCRGFLIRQTFWRRLRAVLTLQAYTRGMIARRLCQRLRAEHQRRLEAERQRLAEEEQLRNQMTVRRAKAEADRKHQERLFQLAQQQEERDREEKEEARRKKELLDQMEREKEQPVDHSDMVDRMFGFLGDSGPLPNQDGQAPAGFEDLEQTPRGEEAEEEMLNEAPPLPDEEEEDLSEYKFSKFAATYFQGLSTHTYIRRPLKQPLLFHEDEGDQLAALAVWITVLRFMGDLPEPKCQIVINDGSEKIPVMTKIYETLGKRTYKRELQELQVEEEVRRSIDSQKRNSVRHKLVSLTLKRKSKITEEVTRRLTEGVDYGLQGNSMLEDRPTSNLEKLHFIIGNGILRPTEETSLLTASTCCPPSPGRSTTSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUT8</accession>
    <name>A0A3B5MUT8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Apolipoprotein D</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027875305.1">
        <property type="nucleotide sequence ID" value="XM_028019504.1"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A3B5MUT8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025032"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025337.1">
        <property type="protein sequence ID" value="ENSXCOP00000025032.1"/>
        <property type="gene ID" value="ENSXCOG00000018686.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114145860"/>
    <dbReference type="KEGG" id="xco:114145860"/>
    <dbReference type="CTD" id="767642"/>
    <dbReference type="GeneTree" id="ENSGT00510000046981"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000302">
        <property type="term" value="P:response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042246">
        <property type="term" value="P:tissue regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19437">
        <property type="entry name" value="lipocalin_apoD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000003">
        <property type="entry name" value="Apolipoprotein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026222">
        <property type="entry name" value="ApoD_vertbrte"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002969">
        <property type="entry name" value="ApolipopD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022271">
        <property type="entry name" value="Lipocalin_ApoD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10612">
        <property type="entry name" value="APOLIPOPROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10612:SF14">
        <property type="entry name" value="APOLIPOPROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036893">
        <property type="entry name" value="Lipocalin_ApoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02058">
        <property type="entry name" value="APODVERTBRTE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01219">
        <property type="entry name" value="APOLIPOPROTD"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00179">
        <property type="entry name" value="LIPOCALIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10" id="KW-0873">Pyrrolidone carboxylic acid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017107520" description="Apolipoprotein D" evidence="11">
        <location>
            <begin position="19"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="12">
        <location>
            <begin position="42"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR036893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="186" mass="21216" checksum="263D7A535A2E2C02" modified="2018-12-05" version="1">MRVFLASIFVLLIPIIWAQVPHWGPCPEPEVQPAFILKQFMGRWFEIAKLPAQFEKGRCIETNFTLTTDNSIRVVSSEILKGEVRKIVGTGVIEDPKNPAKLGITYSYVLPYSPYWILSTDYVNFALVYSCTDVLRLFHVDFAWILGRTRSLPDATVEKARETFATNNIDVTRMIPSRQQGCDKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MV27</accession>
    <name>A0A3B5MV27_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thyroid hormone receptor associated protein 3b</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the BCLAF1/THRAP3 family.</text>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="THRAP3/BCLAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="THYROID HORMONE RECEPTOR-ASSOCIATED PROTEIN 3"/>
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    </dbReference>
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        <property type="entry name" value="THRAP3_BCLAF1"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="309"/>
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            <begin position="672"/>
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            <begin position="11"/>
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            <begin position="63"/>
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            <begin position="101"/>
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            <begin position="127"/>
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            <begin position="728"/>
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    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    <sequence length="782" mass="89133" checksum="CD0CA10C31B8922A" modified="2018-12-05" version="1">MSKVLDSPSRPRSRSRSRSRSYSRSRSRSHSRSRSRKRHYSSRSRSRSHSPGYRNYPSRDYQGNNRGFRGYNRGYRRPYYYRGRNRGGGGGGWHDRQEHHSPRRPYSRSRTPKKRSGSRSRSRYSDRSSSGRSRQSRHSSYSSRSRSRSSSRRHDSKSRKKSKDSKDKQPESQPEKSATAADGTVIAKTSGGKWIDYDASPKRRSPDSKKEDTLDLEAKGSGGQMWKTVGSVSPPGKSPSKSGQTASFSGFGFFSKDDTKPGDSAGISAAFKKYDADPEKSSKSSDIFSISASPYADPKDDKTLPFFDPGEEEFLESQGLKDRIINENADGKPVLTERDIFGKWGDEPNYSTSYQSAKEKGRRDAEEEEAVDEAEEELYRSRKHSSKKEEKSKKKEKKEKEKTKRSLSPPTTSKEKERPLFPGAFPSLEPSPPHRLSASREEFELKMSCLEEMASSSVSKERLAQRDLLLSSKKDPEFRSIFQHIQSAQLRRSPSELFAQHIVSIVHYIKAQHFPSSDMTLSERFAMYQRKAAEAEMMKPRKSPEIHRRIDVSPSAFKRHPHLFEEVDESSYKDKRFKGDIMDLRLDIERRKRFAGKEHDYKRDGGRSPAGSRGPSRERSTDKSGKHHKKSKKSKKKRDRSPSSSSSSSSPSPYPPHYRGKEYLAEGAEHLEEGYGPPRYAPRDYGGPADRGPRDYEGHFPDRGRGRGFVSFSFPRMRGRGWSRGNYPGNSNGNPANLNAPVRPPEEDWDPEYTPKSRKYYLVGFLFLQRAFCGGQQRAAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MV30</accession>
    <name>A0A3B5MV30_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">Protein HGH1 homolog</fullName>
        </recommendedName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Atherinomorphae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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    </reference>
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        <text evidence="1">Belongs to the HGH1 family.</text>
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            <begin position="101"/>
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            <dbReference type="Proteomes" id="UP000261380"/>
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    <sequence length="372" mass="42480" checksum="B80CD87979DD957E" modified="2018-12-05" version="1">MLSDAEAAELLAFLTPSTRPDVKGKATEYILGLSGSRWDGCRFLRSKPDLLAALFALTSDSSVAIVKDCYHILINLSADETLHQVLVCEVQVLPVLLKNLQDPEFLFSDQISTVLSNLSRHDKTCRTVFQVLQEELGLAQLVEIFCNESFNRKARLHYLGLLLSNLTQLPEARSLLMDRDRCVIQRLLPFTQYEASVVRRGGVIGTLRNCCFDHAHHAWLLSNAVDVLPFLLLPLAGPEELTEEENEGLPVDLQYLPEDKQREADPDIRKMLLETLLLLTATKAGRLTLRQKNVYPIVRELHRWEKDVHVGATCEKLVQVLIGDEPQHGMENLMEVDIPQDVEDKLREEDAKEQQELQEEERRRREEEKQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MV66</accession>
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        <recommendedName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
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        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
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        <location>
            <position position="18"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027698.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="49" mass="5583" checksum="9969AAB029A455D3" modified="2018-12-05" version="1">MIIKSGLDDEQFPESLTCHSILELPLYSTKEIMRERLTKALESKGGFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVJ1</accession>
    <name>A0A3B5MVJ1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
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    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PDZK1-interacting protein 1/SMIM24 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVJ1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023845.1">
        <property type="protein sequence ID" value="ENSXCOP00000023564.1"/>
        <property type="gene ID" value="ENSXCOG00000017601.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000271616"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031627">
        <property type="entry name" value="PDZK1IP1/SMIM24"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15807">
        <property type="entry name" value="MAP17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="114" mass="12827" checksum="2E9B39F0B45670C1" modified="2018-12-05" version="1">VSCGNIPLLCTPLYFSAVRSTKRTLQPWLIGVSAAVGFLVIVFILLIGKKLLSIKRFVNHSYFFSNTDGVALYSVSLRLLKNHALMLEGCLETEEKRGYELTQKLDLDVYETNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVT6</accession>
    <name>A0A3B5MVT6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Olfactomedin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVT6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028046.1">
        <property type="protein sequence ID" value="ENSXCOP00000027711.1"/>
        <property type="gene ID" value="ENSXCOG00000020662.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160055"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003112">
        <property type="entry name" value="Olfac-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050605">
        <property type="entry name" value="Olfactomedin-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192:SF13">
        <property type="entry name" value="OLFACTOMEDIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192">
        <property type="entry name" value="OLFACTOMEDIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02191">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00284">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51132">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017418796" description="Olfactomedin-like domain-containing protein" evidence="5">
        <location>
            <begin position="24"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Olfactomedin-like" evidence="6">
        <location>
            <begin position="132"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="75"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="393" mass="44674" checksum="7A0F4A948DDEAF5D" modified="2018-12-05" version="1">IASTRMVLLLLLSLGLTLGSVQSQGSSQDAFIIQYLERRLVQMEVKQDIIQMTKSVLLTHKYVLFLLRSEVKSQVDSVSVRVDRVERELEYLENKIPTQSNIEMEEALLEQQIKAVELEQLKKKAKINVENDCNTVLSQVKSLKIVKKSGDPNGCWFKDPSEGSNKVYLFSGFRNNTILEYKSLKSFTKTSTPPANEVQLPFYWQGTGHVIYNGFLYCHKADTPNEILKVDLLNGTVVDRTLLPGAGRLPVYSLNPNTYLDMAVDELGLWVIHADLEYGGNLVITKLDKVSLAVEYTWDTQCRSSDAEGAFLICGTLYVVYNTRYGGRSTIQCLYDIHDTIHRLVPVMFFPKRYTSHSSIHYHPGDKQLYAWDDGYQTIYKVETRSNAQVTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MW14</accession>
    <name>A0A3B5MW14_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MW14"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027816"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028151.1">
        <property type="protein sequence ID" value="ENSXCOP00000027816.1"/>
        <property type="gene ID" value="ENSXCOG00000020739.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165572"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032835">
        <property type="term" value="P:glomerulus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF341">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4" id="KW-0518">Myosin</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="5">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="157" mass="17257" checksum="4A1CC315277B1066" modified="2018-12-05" version="1">TCPKCPEEERKCRFLAFPSFLLGISQDGLCNKLTSRVMDSKWGGKTESISVTLNTEQACFSRDALSKALYARLFDYLVDVSPSVRPRLRLSLSVSAGVSCVSVRQQGHAEGDGGAEHRSPGHLRLRDLPGKPAFLNLFWDSAPYPSSRSSPPQSKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZ67</accession>
    <name>A0A3B5MZ67_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SLAIN motif family, member 1b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SLAIN motif-containing family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MZ67"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027740.1">
        <property type="protein sequence ID" value="ENSXCOP00000027407.1"/>
        <property type="gene ID" value="ENSXCOG00000020464.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017860"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031116">
        <property type="term" value="P:positive regulation of microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026179">
        <property type="entry name" value="Slain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22406">
        <property type="entry name" value="NASCENT POLYPEPTIDE-ASSOCIATED COMPLEX SUBUNIT ALPHA, MUSCLE-SPECIFIC FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22406:SF2">
        <property type="entry name" value="SLAIN MOTIF-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15301">
        <property type="entry name" value="SLAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="111"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="192"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
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    <sequence length="273" mass="31246" checksum="8244320380CC7272" modified="2018-12-05" version="1">MGNMMRGASDSSKVNTDNPDSSDDDIVVVINDYPSMEISEPIYKMGENQKLRVSLLEAYWWRVRSIDTRKENYIPNSHVVRVYHGWLFEGVGRQKAEELLLLPGNRVGSFLVRESTGERGLYSLSVKHRAVKHYRIFRLDNSWYYISPKLTFQCLEDMISHYSDSADGLCCTLTTPCLSGKAEAPETNPGPPPVVMRHNFDWRKVDRKQLVSTEGHSNNMVSYGVRNSIAAYLSFSGQEDPQKQRGDRRKKKSRSVYVIPESSNANTEYEDDF</sequence>
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        </source>
    </evidence>
    <sequence length="2058" mass="226918" checksum="A79E28D9A26A00AE" modified="2018-12-05" version="1">MLLFSTSHTFPFFPVVPGPPLTLEGEAVGSNGILLSWTMQSDANNIDGYVIRYFFFLLGRIYPDLLSSKMQHDQSNLYFHAFPVAPGLVTNVTAYAQNHTFVVVTWFLPHRINGLITKFAVKAKHVRTGQTVHYSADILSRATPSPLEITASSPPITLSAVPPAASWNVPISVGVDQLRPYTAYLFEVSAFTSDGEGQVASTMVRMPESAPEDPPDNFSVMNMTSRSIYASWNSPSKITGKFSYVLYLYGPTGCFLYDNSTWDMHFLFTGLTPYTMYRVAVQAKAAGEVGPAAQKVIVTPAEAPSAVQNLEAVAEDSVSIRVSWRSPAQPNGPITQYRLQVLADDTLLQDITLTSGSGHVSTDFHGYSFSPEEVVDVLSEELSYLVSDLNPFTEYVFRVTASTTAGEGPAADITEKTSEQVPSSVLEVSYQNISSTSILVNWIPPLNPNGRITHYSVYGLNLHSNQAMKWVTNTTTILISDLDKYTGYKLRVAASTAVGESTLSEDDDVFVFTLEDVPGFCVCHFNTKHFKSSLPIFFSEPDSPPENLSVVDMSPSTATLTWSAPEKANGVIQYYEVFYENDSYFAVMNTTSNRVTLTSLKPFSFYNVSVRAYTRYGNGNQTSITLNLLSGEDVPGSPPYGLSYESISPSEVNVSWYAPLQPNGVVTHYSLELWNATHHLNLTSPANSLHITHLRKYTQYRIVVQAHTRAGPGNYSSEPLNITTLEDEPFDPPQNVTVVNVTTSSVTLMWQPPTEPNGIIVHYTIYYTDNSTVIEKQRSVKVTLFYLFSFAVPSDPVSNLTAQIFSSTIIIVSWDPPMEPNGRPYYILTLQEAGIFPNMSSPGTVAVNKTIKQTTTDNVFLFTRLRKYFPYVLTVTPATGADSSTDDCMFLSLVPSSPPLLLSTRNHSSSSIEVVWQRPLEANGEITEYTLTLSGPGGTNTTQVPNTSVILTKLASFTAYNLTIIASTRKGAGPSLLVQLHTDEAGPSSPPRNLTIYNHTAVSVWLTWEPPLEPNGVVIKYGFRIQDLITQTVTHRNSSGSSTTEHLSGFRPHSSYEISVYSYTRVGHGNQFSSPVTFTTNESVSDAVGNLSCSGLSWDSVELSWEVPANPNGQILFYEITAEVDMQTFNHQAYAPQYKVAGLSPDQEYAFTVAAVNSAGPGNSLNCTAFTLSESVPTAPRFLSISEVTATNVTLQWTAPVSVPGLLKEYHVVAQLISAVCEPNTQAVVLPTPEDSMALHCVEDNFNVSVNASDGAEEASVILQSLAKYRYYRFKVAAVTNAGPGEYAEWIYAYTLAGNPDAPPRDLKVTPTSTSLQITWEVPAVLSGPTSYLVQVDGPGVNISIVRVPGELTTVFVTNLTAFNLYAVTVTAFTGSVEDASSDGKAIGPIVFQTLEEEPKDPPKNVTVSAVPERVNLVRVSFSPPEKPNGNITAYYVLVYEKDQLVKNMSLDYIHGEPNMLFAFVEGLKGGHTYSIQIAARNGAGRSPLSPHVQITTGIKAPSKPTQRPQAVLGQGGVAMVTHRSITIHMPACFYSDDNGPIAKIQVIVAESGVRDSENLTNWKGAYFHHPAPYLTDAGFPNPTCLARDRAAVMLIEDGRSVSGDDPSLLNHTVTGTYMIGENDNCMGHNDTDHFCNGPLKSNTVYVFKFRATNIKGQYTDSEFSEYIRTTGRSSRKDQALITRLKCILSLLSTFVSRIHQRKKEGGTYSPREAEIIETKCKLDQLIAVADMELKQEKLNRPVNKKSFLQHVEDLCANDNAKFQEEFSDLPKLLPDLATTDADLPWNKSKNRFLNIKPYNNNRVKLLSEPGMAGSDYINASFVSGYLCPNEFIATQGPLPSTVADFWRMIWETGTRTVAMLTQCYEKGRIRCHKYWPEDNKPMTVFSDILISKVSEEVLPDWTVRTLKVEKHGHYILVKHFNYTSWPEHGVPESCSTLIKFVKAVRAHRHDNSTIVVHCSAGVGRTGVFVALDHLIQHVRDHDFVDIYGLVAELRSERMCMVQNLAQYIFLHQSTLELLNNKGNSQSIWFVNYSALEKMDSLDAMEGDVELEWEETTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N1Y6</accession>
    <name>A0A3B5N1Y6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N1Y6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027900"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028236.1">
        <property type="protein sequence ID" value="ENSXCOP00000027900.1"/>
        <property type="gene ID" value="ENSXCOG00000020803.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158165"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06967">
        <property type="entry name" value="NR_DBD_TR2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06952">
        <property type="entry name" value="NR_LBD_TR2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000012">
        <property type="entry name" value="Nuclear receptor subfamily 2 group C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000015">
        <property type="entry name" value="Nuclear receptor subfamily 2, group C, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048245">
        <property type="entry name" value="NR2C1/2-like_DBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048246">
        <property type="entry name" value="NR2C1/2-like_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="12">
        <location>
            <begin position="77"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="13">
        <location>
            <begin position="315"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="574" mass="63031" checksum="903EF985E83EB78D" modified="2018-12-05" version="1">LESSSPGKQQFILANADYSPGGKVILAKQECSPNKVILASSDGSGVNQLLFAAPELAGQQIQFVTDSSDQSIIKPVVEYCVVCGDKASGRHYGAVSCEGCKGFFKRSIRKNLVYTCRGSGECAINKLHRNRCQYCRLQRCIALGMKQDSVQCERKPVEVATREKSMNCAASTEKIYIRKNLCSPLAATPTFVSEKETARSTSLLESSMLLNIQQPFPKLENTILIPASPDKDDLSQGDLGTLANVVSSLAHLNKARELSDGGNDVLGAETLSNGDGSMSDIKVEDHTASDITRAFDTLAKVLHPDDGSAGESLETTMQLMSGDQSGPVVELDGPVLSESHIPFKLMMPLPVPEYLNVNYICESASRLLFLSMHWARSIPAFQALGGQDNDINLMKACWNELFALGLAQCSNIMNVGTILSAIINHLQTSLQEEKLSPERGKVVMEHIWRMQEFCNSMSKLSPDSYEYAYLKAIVLFSPDHPGIDNIPQIEMFQEKAYMELQDYVTRTYPEDSYRLMMLLSKLLLRLPALRLISAAVTEELFFAGLIGNVQIDSIIPYILKMESTDYNSQAGSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KJI7</accession>
    <name>A0A3B5KJI7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Translocon-associated protein subunit alpha</fullName>
            <shortName evidence="11">TRAP-alpha</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Signal sequence receptor subunit alpha</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9 11">TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotetramer of TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">Shows a remarkable charge distribution with the N-terminus being highly negatively charged, and the cytoplasmic C-terminus positively charged.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TRAP-alpha family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KJI7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000323"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000330.1">
        <property type="protein sequence ID" value="ENSXCOP00000000323.1"/>
        <property type="gene ID" value="ENSXCOG00000000113.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00400000022103"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005595">
        <property type="entry name" value="TRAP_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12924:SF2">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12924">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03896">
        <property type="entry name" value="TRAP_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="240"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="10"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="268" mass="29879" checksum="1CF84963E4007668" modified="2018-12-05" version="1">SGPAVSAQDLTEDEEAEAVDEDVVDDVTAEDEDDEAEVEDDENTELVRFGLTSTSLLGPVRDIKHVKYFPANNIVKFLLGFTNKGGENFMVESLDASFRYPQDYQFYIQNFTALQLGTVVPPGRQATFEYSFIPAEPMGGRPFGLVINLNYKDSSGNVFQDAVFNQTVTITEREDGLDGETIFMYVFLSGLGLLVIVGLHQLLESRKRRRPAPKVETGTSSHNDVDLSWIPQETLNQISTFSIRSPDKASPRRSPRKRNQKRSAGSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KPG4</accession>
    <name>A0A3B5KPG4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Plakophilin 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PKP4</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-catenin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KPG4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000357"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000364.1">
        <property type="protein sequence ID" value="ENSXCOP00000000357.1"/>
        <property type="gene ID" value="ENSXCOG00000000299.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155773"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000008">
        <property type="entry name" value="plakophilin-4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028435">
        <property type="entry name" value="Plakophilin/d_Catenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372:SF8">
        <property type="entry name" value="PLAKOPHILIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372">
        <property type="entry name" value="PLAKOPHILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="415"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="459"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="9">
        <location>
            <begin position="717"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="119"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="275"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="618"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="927"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="78"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="145"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1036" mass="114650" checksum="DDCE1BD8827C0E5E" modified="2018-12-05" version="1">MNSYSDSGYQDASSGYLSRQNQGKAELKMQHSFPGAGTGTLLRNARAEGQVPGVTAALPGRAMRRVSSVPSRSHSPAYASSISPSRGSLRTSVGSAYGSPVVTEPKPLSNIHFTTLPSVQRTGSIGSGSPYSPQKTSPAGLRRISSTNSRPGSTSRTTSPYQTSAGSASGRMGSPLTLVDPPLIKQPTHSSSPVRASMTAVPQHYSSTLPRSMLHNTDPYGPQSYDIYERMTRPDSLAGIRSSYASQHSHHGQDMRSTISPDRHIAPIYEDRTFRGPLYRSPSHTQQGTLYRSGSGVGSLQRTSSQRSAMTYQRNNYALNTAANYADPYRSAQYRPSDSNYARQTIVMDDGAPRSPSIDSIQKDPREFAWRDPELTEVIHMLQHHFPSVQANAAAYLQHLCFGDNRVKSEVCRLGGIKHLVDLLDHKTVEVQRNSCGALRNLVYGKAMDENKIAVRNAGGIPALLRLLRKTVDAEVRELVTGVLWNLSSCDAVKMTIIRDALSTLTNTVIIPHSGWSSSTFDDDHKLKFHSSLVLRNTTGCLRNLSSAGEEARKQMRTCDGLVDSLLYVIKACVNTSDFDSKIVENCICTLRNLSYRLELEMMPSSFPVNQEMDGLLGRESPSKEVDSSCWGRKRKKKKNSLQEDSWDGVGPIPGFSKSPKGAEMLWHPSVVKPYLTLLAESSNPATLEGSAGSLQNLSAGNWKFAAYIRAAVRKEKGLPILVELLRMDNDRVVCSVATALRNMALDIRNKELIGKYAMRDLVNRLPGGNTTLLSDETVAAICCTLHEVTSKNMENAKALADTGGIKKLVDITKGRGDRYSVKVVKAAAQVLNTLWLYRDLRPIYKKDGWNQNHFITPVSTLERDRFKSQPTLATSTVQMSPVNHPAASATSSPAILGIKEHRSSVRDYQRTQSTMQFYNYQGDGSIHRKQYTGSGKPSPYYYSSPTREEPRRSQPVYYTEEPSRRNYDTYRMYLQHPHGYDDAYLEEVITYPPTADYSSQPHGLKSTSNYVDYYASTRRPSYRAEQYPGSPDSWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KPK7</accession>
    <name>A0A3B5KPK7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Laminin subunit alpha 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KPK7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002068"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002098.1">
        <property type="protein sequence ID" value="ENSXCOP00000002068.1"/>
        <property type="gene ID" value="ENSXCOG00000001657.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155362"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000069">
        <property type="entry name" value="Laminin subunit alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000315">
        <property type="entry name" value="Laminin subunit alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000017">
        <property type="entry name" value="Laminin subunit alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000026">
        <property type="entry name" value="laminin subunit alpha-2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF291">
        <property type="entry name" value="LAMININ SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="7 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="565"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin N-terminal" evidence="13">
        <location>
            <begin position="13"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="12">
        <location>
            <begin position="392"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="12">
        <location>
            <begin position="447"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="392"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="466"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="599" mass="67388" checksum="2351867C4EF15241" modified="2018-12-05" version="1">PYAEYKVYVLLFCFSGLFPAVLNLATMADITTNATCGTNGPEMFCKLVEHVPGQPVRNPQCRTCNQRSTNSFEHHPIEYAIDGTNRWWQSPSIMNGMEYHYVTVTLDLKQVFQVAYVIIKAANSPRPGNWILERSIDGVTFEPWQYYAITDTECLTRFNIYPRTGPPSYTRDDEVICTSFYSKVHPLENGEIHTSLINGRPSADDPSPTLLNFTSARYIRLVFQRIRTLNADLMTLTLSDPKDIDPIVTRRYYYSIKDISVGGMCICYGHAKACPLNSATKFSCECEHNTCGESCDHCCPGYHQQPWMAGTFLTRHVCEKCNCNNKAEECFFNQTVADLSLSLDTHGQYRGGGVCIGCRDNTAGINCQSCIPGYYRPTKVCRIYEEDPCIPCSCDPHGSISQTCVADESQASPGQPAGSCWCKEGFGGLRCDRCAVGYKDYPTCERCNCRVEGSINADPCVTPCICKENVEGENCDRCKLGFYNLQQDNRRGCEKCFCMGIASQCSPSTWNFENVRGRVWSVHRETPSYLTIRHLDVVNDLGSAYYWNAPEIYLGNKVRADKRIFLYYCIPSVLLMIFVIFVNFFAAVLFCCSSLSCSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQ89</accession>
    <name>A0A3B5KQ89_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027890795.1">
        <property type="nucleotide sequence ID" value="XM_028034994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQ89"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000637"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000647.1">
        <property type="protein sequence ID" value="ENSXCOP00000000637.1"/>
        <property type="gene ID" value="ENSXCOG00000000555.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114155204"/>
    <dbReference type="KEGG" id="xco:114155204"/>
    <dbReference type="GeneTree" id="ENSGT00390000012710"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022829">
        <property type="term" value="F:wide pore channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001771">
        <property type="term" value="P:immunological synapse formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001913">
        <property type="term" value="P:T cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052784">
        <property type="entry name" value="Perforin-1_pore-forming"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096:SF3">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017479542" description="C2 domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="128" mass="14527" checksum="D6903AA53A7ACACF" modified="2018-12-05" version="1">MVSKLHVVLLVLCALSLAQAQLRLFDLRASKLPSNLFGTADGYVKVFCGETSLGKTAVRKDTANPWWEEEFNYFHALENDEMKLEVYDNDLLFDDQLGVCQRQLQPGTHQHDCFLKKGGTLHYSYTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KR43</accession>
    <name>A0A3B5KR43_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KR43"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000357.1">
        <property type="protein sequence ID" value="ENSXCOP00000000350.1"/>
        <property type="gene ID" value="ENSXCOG00000000316.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="64" mass="7470" checksum="8C205DEC50AB95BF" modified="2018-12-05" version="1">MSLHGNDFFWKGKDRKYFRIFWGEGQPDNVNGSEDCAQILEVNKTWNDNKCDGSFPWICEKAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KR90</accession>
    <name>A0A3B5KR90_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anaphase-promoting complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cyclosome subunit 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Together with the RING-H2 protein ANAPC11, constitutes the catalytic component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 drives presynaptic differentiation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1 and FBXO5. In the context of the APC/C complex, directly interacts with UBE2C and UBE2S. Interacts (via cullin domain) with ANAPC11 and with UBCH10. Interacts with NEUROD2. Interacts with FBXO43; the interaction is direct.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KR90"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000156"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000161.1">
        <property type="protein sequence ID" value="ENSXCOP00000000156.1"/>
        <property type="gene ID" value="ENSXCOG00000000153.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016127"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000284">
        <property type="entry name" value="Anaphase-promoting complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000027">
        <property type="entry name" value="Anaphase-promoting complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.130:FF:000008">
        <property type="entry name" value="anaphase-promoting complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044554">
        <property type="entry name" value="ANAPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014786">
        <property type="entry name" value="ANAPC2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057975">
        <property type="entry name" value="TPR_ANAPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45957">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45957:SF1">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08672">
        <property type="entry name" value="ANAPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25773">
        <property type="entry name" value="TPR_ANAPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01013">
        <property type="entry name" value="APC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="15">
        <location>
            <begin position="382"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="341"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="348"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="704" mass="79922" checksum="EB9C1388F1F52747" modified="2018-12-05" version="1">MEEMVVELNSGEPSAPGSDQGVAKAWESVTAALVSGTEPRFGAVSPGSSLSEQDRLNLALLCSHGLGRLLGVWLLESLQVRLSSSVVPEFWSGLEQPQDQLEERDRAGVLLSAFQTLLDRLDPFLSYAEAVTAILHRLIEQRMEQHCRGEYERSFLLDFQEWLELVLGWLGKVFATEGDGATPVPMDPSVPSTPSACGSPVLKQWRCHMHQFFCRIYVNMRIEELFSIIRDFPESKPAIEDLKFCLERTNQRQQLLTSLKSAFESRLLHPGVHTSDILTVYISAIKALRELDPSMVILQVACQPIRKYLRTREDTVRQIVAGLTGDAEACSDLASELSRGDPVTLEMQDSDDEGNDPEDWAPDPTDAVPDKLGSKRRSSDIISLLVSIYGSKEIFIDEYRTVLADRLLHQLNYNTAREIRNVELLKLRFGESHMHYCEVMLKDMADSRRINSHIREEESKLSEEEQPPLALSAIVLSSEFWPTLKEEKLELPAAVCHAMEAYTRRYEKLKAMRTLSWKPHLGSVTLDLELEDRTLTNLTVSPFHAAIILHFQEKSSWTLEELSVKLGAPKELLHRKLALWQQQGVLREEAAGRYVVMETGATRDRLDRGVMLIDSDEERDSNTTTQSEQREEKLQLFWAYIQAMLTNLDSMTLERIHTMLRMFVATGPVVTEMDVNELEAFLQRKVREHQLIVSTGIYRLPKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYC2</accession>
    <name>A0A3B5KYC2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PWWP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KYC2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005108"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005169.1">
        <property type="protein sequence ID" value="ENSXCOP00000005108.1"/>
        <property type="gene ID" value="ENSXCOG00000003996.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000172841"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00855">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50812">
        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="2">
        <location>
            <begin position="58"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="152" mass="17332" checksum="35D6A2F2F767FC6A" modified="2018-12-05" version="1">PRPATRAASARPCRPPPPPSNTRLSTATSSQTTLPPPPPHTQPSGSRKKPPPPVKFAEGEIVWAKFGRRPWWPCEVLADPALGVHHRMKEPCDRLCRLYHVRTFGEPKEFVWVEEKSTQAFHGGFEFDQLLLLRRRSKQREQNNRNTVSVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYD4</accession>
    <name>A0A3B5KYD4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KYD4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005118"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005179.1">
        <property type="protein sequence ID" value="ENSXCOP00000005118.1"/>
        <property type="gene ID" value="ENSXCOG00000004000.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160408"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052420">
        <property type="entry name" value="Espin/Espin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24153">
        <property type="entry name" value="ESPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24153:SF14">
        <property type="entry name" value="ESPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00246">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017402602" description="WH2 domain-containing protein" evidence="4">
        <location>
            <begin position="25"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="WH2" evidence="5">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="222" mass="25115" checksum="CC0E82DCBC74AD5A" modified="2018-12-05" version="1">DFPPVLPFTKLMLSLISLPLLVEALPHRENRCSGGTKSFNMMSPTGDNSELLAEIKAGKSLKPTPHSKGYTTVFSNTGTTGNNVGPNPSPTAGAAQPESERKTSLADIEALVPIHDEQGKPIPEWKRQVMVRKLQAKMQEEEEYKRKAEEEAQRLASMPAWRRDMMKKKMDEERKAEQQAKQAKECEEKQELERLRTMGYDETKLAPWQRQIILKKGDIAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZW5</accession>
    <name>A0A3B5KZW5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aminoacyl tRNA synthase complex-interacting multifunctional protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Multisynthase complex auxiliary component p38</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Protein JTV-1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Required for assembly and stability of the aminoacyl-tRNA synthase complex. Mediates ubiquitination and degradation of FUBP1, a transcriptional activator of MYC, leading to MYC down-regulation which is required for aveolar type II cell differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Part of the multisynthetase complex (MSC), a multisubunit complex that groups tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18. Interacts (via N-terminus) with KARS1. Interacts with EPRS1. Forms a linear complex that contains MARS1, EEF1E1, EPRS1 and AIMP2 that is at the core of the multisubunit complex. Binds FUBP1 (via C-terminus). Interacts in both its unphosphorylated and phosphorylated forms with p53/TP53 (via N-terminus) in the nucleus following UV irradiation. Interacts (via N-terminus) with PRKN/parkin (via first RING-type domain). Interacts with TARS3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027898627.1">
        <property type="nucleotide sequence ID" value="XM_028042826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZW5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001104"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001117.1">
        <property type="protein sequence ID" value="ENSXCOP00000001104.1"/>
        <property type="gene ID" value="ENSXCOG00000000914.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114160291"/>
    <dbReference type="KEGG" id="xco:114160291"/>
    <dbReference type="CTD" id="7965"/>
    <dbReference type="GeneTree" id="ENSGT00390000015826"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042360">
        <property type="entry name" value="AIMP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031889">
        <property type="entry name" value="AIMP2_LysRS-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041503">
        <property type="entry name" value="AIMP2_thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13438">
        <property type="entry name" value="AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13438:SF2">
        <property type="entry name" value="AMINOACYL TRNA SYNTHASE COMPLEX-INTERACTING MULTIFUNCTIONAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16780">
        <property type="entry name" value="AIMP2_LysRS_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18569">
        <property type="entry name" value="Thioredoxin_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="AIMP2 lysyl-tRNA synthetase binding" evidence="17">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="AIMP2 thioredoxin-like" evidence="18">
        <location>
            <begin position="119"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Glutathione S-transferase C-terminal" evidence="16">
        <location>
            <begin position="248"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF18569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="322" mass="35124" checksum="89EAAE8BB706C33A" modified="2018-12-05" version="1">MSMYQVKPVSPDIKIDLPTCMYKLPNIHTRQENSCSSDHVLQNGDMDPAVRALEARQDEIMRKLYELKAAVEGLAKTVTTPDADLDVTVSSSLSSQSSSCDIFKATKNLDALLGKDLGALRDIVINANPAQPPLTLLVLHSLLCQHYRVLSTVHVHSSVSSVPPQLLSCLGPKHADSYARHMFQLGFTLIWKDVPKLEMKFSIRNMCPIEGEANVARFLFKLLAPYPSDAAVATLVDTWVDIAFYQLADGSAKEQAAVLRALGSALGHAPWLCGPEFSLADIACYCCLSQNGSTSATAANIQRWIKSCENLGHFGPAKLLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1E4</accession>
    <name>A0A3B5L1E4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cadherin 15, type 1, M-cadherin (myotubule)</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1E4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004155"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004200.1">
        <property type="protein sequence ID" value="ENSXCOP00000004155.1"/>
        <property type="gene ID" value="ENSXCOG00000003270.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160118"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042074">
        <property type="term" value="P:cell migration involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060027">
        <property type="term" value="P:convergent extension involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007398">
        <property type="term" value="P:ectoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007498">
        <property type="term" value="P:mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001841">
        <property type="term" value="P:neural tube formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000011">
        <property type="entry name" value="Cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000019">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000022">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF300">
        <property type="entry name" value="CADHERIN-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cadherin" evidence="11">
        <location>
            <begin position="80"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="11">
        <location>
            <begin position="187"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="11">
        <location>
            <begin position="299"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="429"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="438"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="453" mass="50799" checksum="E2EFBA1B9A8A9D65" modified="2018-12-05" version="1">MQVPSYCSLDKHFKFIVASTTSAIYLKLYFSCNFDLTYCQPCFLQVCSSAEYHHNEAEQDLNALYLWRNKGKGMIRVKRDWIIPPIRVSENSRVVPEDLVQIKSDKIFSGEVIYKLEGPGVDQEPKNLFEIDDKTGMIRSKRPLDREQHSSFTLKAFALSPSGERLENPTTIEIVVLDQNDNKPVFTQKQFFGSVHEFSVPGTLVMSVSATDADDPMTENAALGFSIVGQESIPPNAVNKTMFGINNQTGAIYTRDVGLDREEFKLKLRVADMGGDGLISDAVAIITVSDINNHAPQFHPTSYNIKALENVYNVEIGRVSVIDRDDPGTGNWEVKYTVSHDPFGNFAIQTDPVTNQGILTVVKPLDYEVKREHNLILTVENINSLSIKVPNLRLSSATVSITVVNENEAPYFKENPILIQVPENEKPGKLLKSNIASDPDHSELRYERKALQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1V0</accession>
    <name>A0A3B5L1V0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1V0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003861"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003904.1">
        <property type="protein sequence ID" value="ENSXCOP00000003861.1"/>
        <property type="gene ID" value="ENSXCOG00000003031.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010427"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090694">
        <property type="term" value="C:Scc2-Scc4 cohesin loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061775">
        <property type="term" value="F:cohesin loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140588">
        <property type="term" value="P:chromatin looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048565">
        <property type="term" value="P:digestive tract development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048703">
        <property type="term" value="P:embryonic viscerocranium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034087">
        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071169">
        <property type="term" value="P:establishment of protein localization to chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033031">
        <property type="entry name" value="Scc2/Nipped-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704:SF18">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN DELANGIN SCC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="110" mass="12322" checksum="5060FBE2A363B56B" modified="2018-12-05" version="1">MDSADDTGQTVELMDIVAICRPKYKDRPQIAKIVQKTRSGYSVHWMTGSYSGPWTVAKKRDGRKKVPWVDSIKESDIIYKKISLTSGQKLTNKVAQTLRALYAAKDGSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2F1</accession>
    <name>A0A3B5L2F1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cadherin 16, KSP-cadherin</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2F1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004046"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004090.1">
        <property type="protein sequence ID" value="ENSXCOP00000004046.1"/>
        <property type="gene ID" value="ENSXCOG00000003173.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165234"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000242">
        <property type="entry name" value="Cadherin 16, KSP-cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000243">
        <property type="entry name" value="Cadherin 16, KSP-cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000253">
        <property type="entry name" value="Cadherin 16, KSP-cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000355">
        <property type="entry name" value="Cadherin 16, KSP-cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000418">
        <property type="entry name" value="Cadherin 16, KSP-cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF424">
        <property type="entry name" value="CADHERIN-16 ISOFORM X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="724"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="7">
        <location>
            <begin position="76"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="7">
        <location>
            <begin position="218"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="7">
        <location>
            <begin position="285"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="7">
        <location>
            <begin position="401"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="7">
        <location>
            <begin position="508"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="767" mass="85284" checksum="949DE884B2B27D96" modified="2018-12-05" version="1">VKIGVPENYDGIFPWYLTKYSDLVLTGDDEGIFGVDAQFLYAIKPLDREKQPSYSLQTFTVEVFVIDKNDNVPTFIEESMRGSVQLGLLKIPFMRVEAVDRDDRNTAHAELRFSMMEQTPRIPSSQMFFIDSITGEVSLTEEGASTLDPEMCGRYKLSVMVKDMAGRTNAFFTSGIVTVEVTGNTWASPDPVRLQENLPGPYPIPISQVKWSGSQAAYSLDGEFPEMLFTISREGVIYLNAPLDRETQDQFHISIVVERPDGRQIAKPVELRVMVGDANDNRPTFPQAQYHTVVKELAARTEILTVQAADNDDPKTDNVRIFYRLVGQIPESPRALFRVDRDSGVISVQADSMEGTAPQYTLTITAEDAKLNSSCTVVVTVQDENNNPPVFSQHEYGPFHIPEDALVGTTVTSVLARDADVRGGDSWQVDYRLESGNEEEVFTLVTDRQTNEVSLILSKVLDFERESEYILVLSAQNQVPLVRGRYGPLSTATVSIYVDDVNEGPVLSQSHYEVTVREGEEPGRVIATIRGYDPDSHPIYSLQGDTKKYFSIGKYSGELKTVQALDREENSTYTMEVIAVDGNSSLSASTLVTVQILDVNDNSPVLVGDYSWKYLCTPRWEDQALVLASRDSDGPQHGGRLNFSLRSDATVRRNWKLTPINTHTNLSLNIPNLPPEVYMVPFTISDSSSPPRSTFINLPCPCNVRGNCKIAAKPLQGMPTIQSAVGLLLGTFAVIIILIVIFVRLSYQNPKEPSKTSQERVPLKISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2K5</accession>
    <name>A0A3B5L2K5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">IPT/TIG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Forms either a homodimer or a heterodimer with a related family member.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the COE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2K5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004101"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004145.1">
        <property type="protein sequence ID" value="ENSXCOP00000004101.1"/>
        <property type="gene ID" value="ENSXCOG00000003166.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182859"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11606">
        <property type="entry name" value="COE_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01175">
        <property type="entry name" value="IPT_COE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4280:FF:000001">
        <property type="entry name" value="transcription factor COE1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3180:FF:000001">
        <property type="entry name" value="transcription factor COE1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001696">
        <property type="entry name" value="Transcription factor COE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3180">
        <property type="entry name" value="Transcription factor COE1, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032200">
        <property type="entry name" value="COE_DBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038173">
        <property type="entry name" value="COE_DBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032201">
        <property type="entry name" value="COE_HLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038006">
        <property type="entry name" value="COE_IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003523">
        <property type="entry name" value="Transcription_factor_COE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018350">
        <property type="entry name" value="Transcription_factor_COE_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10747">
        <property type="entry name" value="TRANSCRIPTION FACTOR COE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16422">
        <property type="entry name" value="COE1_DBD"/>
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    <keyword evidence="6 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="248"/>
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            <dbReference type="SMART" id="SM00429"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="587" mass="63974" checksum="3B406CCDFAB4F5C2" modified="2018-12-05" version="1">MFGIQDSLPRGAAAGLKEEPLAVRSWMHSAGMVDANTAAQSGVGLARAHFEKQPPSNLRKSNFFHFVLALYDRQGQPVEIERTTYVDFVEKEKPSGEKTNNGIHYKLQLLYSNGVRTEQDLYIRLIDSMTKQAIIYEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRRFQVVVSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEATPCIKAISPSEGWTTGGATVILIGDNFFDGLQVVFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGAPGRFVYTALNEPTIDYGFQRLQKVIPRHPGDPERLPKEVLLKRAADLVEALYGMPHNNQEIILKRAADIAEALYSVPRNHNQIPSLGNSTSHGMMGVNSFGGQLAVNVSETGQGTMGYNRNTSSVSPRGYVPSSTPQQSNYGNTVSSSMNGYGNASMPNLGVATSPGFLNGSSANSPYGIVPSSPSMAVSNCNSSHGVFSFSAAVKQKSAFAPVVRPSASPPPPPNCSGGNANGLQGEAPPSLLPRWPTSPASVKLYLMFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L3N9</accession>
    <name>A0A3B5L3N9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Nucleolin</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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    </dbReference>
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        <property type="entry name" value="NUCLEAR RNA-BINDING PROTEIN SART-3"/>
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    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="514" mass="55331" checksum="FD2BD86DDFCC8DC6" modified="2018-12-05" version="1">AAAKPAKGPAKPVVKTQSAKESSDEEDSEEEAPPPKKATPAKPAAKAPAKAAAAEESSDDEDSEEEAPPPKKTPAAKQSAKPAAKAQPAEESSEEDDDEDDFPPVFFYYYLFFVTASLKFLPSSTEAPVKPAKRKAEGKKETPPAKKVKSDGEGLSLFVGNLNCNKDFEEIKDGLRKFFSKNDIEVTDVRLGMSKRFGYVDFANEEDLNKALELNGKKFMGQEVKLDKARSKESSQEGKKERDARTLFVKNLPYSATIDDLKELFESAVDVRLPQGQTGSNRGKKKESILSSKIQCSTHMFNTQWFLSVLFVSFPFTLVTNLLYQGLVFVFRFAFVEFENAEDAKEALENLNHTEIEGRSVRLEFSQNSGGRDGGRGNSGPTKTLFVKGLSEDTTDQSLKDSFEGSVGARIVTDRDTGASKGFGFVDFDNESDCKAAKEAMEDGEIDGSKVTLDYAKPKGEGGFRGGRGGGGGGGRGGFRGGRGGGFGGFGGFGGRGGGGFGAKPQGKKIKFDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L3Y0</accession>
    <name>A0A3B5L3Y0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Superoxide dismutase</fullName>
            <ecNumber evidence="13">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 13">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
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    </dbReference>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="P:innate immune response"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001780">
        <property type="term" value="P:neutrophil homeostasis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:response to cadmium ion"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051597">
        <property type="term" value="P:response to methylmercury"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Superoxide dismutase [Mn], mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050265">
        <property type="entry name" value="Fe/Mn_Superoxide_Dismutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11404">
        <property type="entry name" value="SUPEROXIDE DISMUTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11404:SF6">
        <property type="entry name" value="SUPEROXIDE DISMUTASE [MN], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0944">Nitration</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017380032" description="Superoxide dismutase" evidence="14">
        <location>
            <begin position="28"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase N-terminal" evidence="15">
        <location>
            <begin position="42"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="16">
        <location>
            <begin position="130"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000349-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="239" mass="26770" checksum="0E2003B78A01BCFC" modified="2018-12-05" version="1">MEKFSLHVSSTTGLFFSLFCFLSRCAASLSQSICKVAASRQKHTLPDLTYDYGALEPHINAEIMQLHHSKHHATYVNNLNVTEEKYEEALAKGDVTAQVALQPALKFNGGGHINHTIFWTNLSPNSGGEPQGELMEAIKRDFGSYQKMKEKMAAVTVAVQGSGWGWLGYDKESGRLRIAACANQDPLQGTTGLIPLLGIDVWEHAYYLQYKNVRPDYVKAIWNVINWENVQERLQIAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4L0</accession>
    <name>A0A3B5L4L0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L4L0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002734.1">
        <property type="protein sequence ID" value="ENSXCOP00000002699.1"/>
        <property type="gene ID" value="ENSXCOG00000002145.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164468"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF296">
        <property type="entry name" value="PROTOCADHERIN 1 GAMMA 11 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017388242" description="Cadherin domain-containing protein" evidence="12">
        <location>
            <begin position="25"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="22"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="129"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="238"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="598" mass="65972" checksum="FB30519436809A46" modified="2018-12-05" version="1">MLERTMRWQVLLFVVIFALDCVAGQATYSIPEEMSKGSLVGNIAHDLGLDVKRLKSGRARIYTEDKAEYIELNTDRGLLVVKERIDRETLCGQTTPCALHLQITLEDPIEFYSVNVEINDINDNVPTFKKEEMKFRISESAVIGAKFVLERAMDLDVGVNGLQSYSLKPTDNFILKLNSQQDGSKKVEMVLQKPLDREAQDHIALTLTASDGGEPQLSGTMRVEIVVLDVNDNAPVFTQETYKVTVMETAPRGTILSTVRAVDADEGSNGKVVYSITNTLDDVPNLFQVAEESGVVSLIGSLDYEKTQHAGHRGPALEPGLERGHDGERLVAGLLPMEPGRAQPCCLAYGPNNSSDYPPFLESLEGLLESAPPGDSLVLLGDFNAHVSNASETWRGMVGGNTIPRSSDVLLLDFCAHHGLSITNTMFRHKGVHTCTWHQDTLGRSSMIDFVNVSLDLRPCVLDTQVKRGAELSIHHYLVVSWLRWWGRMPVRPGRPKRAVRVCWERLVESPVRLSFNSHLRQNFEHVPGEVGAMESEWTILGASIMDTAYRSCGRKVVGACRGGQPSNPLVDAFGEGCRQAEEGVLLGLFGLWDSKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L546</accession>
    <name>A0A3B5L546_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Gamma-aminobutyric acid receptor subunit rho-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">GABA(A) receptor subunit rho-1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">Three rho subunits (rho-1/GBRR1, rho-2/GBRR2 and rho-3/GBRR3) coassemble either to form functional homopentamers or heteropentamers. Rho-1/GBRR1 subunits can also associate with alpha-1/GBRA1 subunits to form a functional GABAAR. Interacts with SQSTM1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRR1 sub-subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L546"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005937"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006003.1">
        <property type="protein sequence ID" value="ENSXCOP00000005937.1"/>
        <property type="gene ID" value="ENSXCOG00000004603.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158591"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    </evidence>
    <sequence length="452" mass="52524" checksum="EFDFE064FBED6BEE" modified="2018-12-05" version="1">MCSIHWPNTDCKYKVYISQTFCFYFPNSPIYKRSPDLTKNPMTKSEQLLRIDDHDFTMRPAFGGPPIPVGVDVQVESIDTISEVDMDFTMTLYLRHYWKDERLSFPSTNNQSMTFDSRLVKKIWVPDMFFVHSKRSFIHDTTTDNVMLRVYPDGNVLYSLRVTVTAMCNMDLSRFPLDTQTCSLEIESYAYTDDDLMLYWKKGNESLNTDDRISLSQFLIQKFHTTTKLAFYSSTGWYNRLYIHFTLRRHIFFFLLQTYFPATLMVMLSWVSFWIDRRAVPARVPLGITTVLTMSTIITGVNASMPRVSYIKAVDIYLWVSFVFVFLSVIEYAAVNYLSTVQERKERKLRERLPCTCGIGNPDEMMIDPQITGYGSMDVNTTGNYGIPENGGRQERILAQVALNDPQITGQVKASRSYVNIWIDTHAIDKYSRVVFPGAYILFNIIYWSIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9G0</accession>
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        <submittedName>
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        </submittedName>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
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        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
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            <begin position="23"/>
            <end position="134"/>
        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004454.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="134" mass="15453" checksum="3A6BF9F05D774417" modified="2018-12-05" version="1">MSWSFVVIFVFILLLFVGYCSEKTNSKGCANLTLVLDNWKYAIMTQVKDLLLHDHNTVLPDYGRIQPLSEALGDLYKEFNSLKERLVGLTARFGDVESFVDDARGLWWGRRRETVQFDGLKKSPLFIFDAEPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9N0</accession>
    <name>A0A3B5L9N0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-directed primase/polymerase protein</fullName>
            <ecNumber evidence="7">2.7.7.102</ecNumber>
            <ecNumber evidence="2">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PRIMPOL</name>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Poeciliinae</taxon>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <reaction evidence="8">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
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            <dbReference type="ChEBI" id="CHEBI:61560"/>
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    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate.</text>
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        </reaction>
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        <text evidence="1">Belongs to the eukaryotic-type primase small subunit family.</text>
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        <property type="gene ID" value="ENSXCOG00000005560.1"/>
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        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:chromatin binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="P:response to UV"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="DNA-DIRECTED PRIMASE / POLYMERASE PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="DNA-DIRECTED PRIMASE_POLYMERASE PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="Herpes_UL52"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="420"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="424"/>
            <end position="435"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00009762"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="481" mass="55459" checksum="8F989568A0F32895" modified="2018-12-05" version="1">LRMSRWGERLKKVERLAQSFQQNPLTTPYKPRLWPYQPSSVWKLFPRQNLAISFAHSCKEAVHVFALEKEKASLGQRIYLVTSYSELWHYYRVYPQSLMHCYEVIPEGAVCKLYFDLEFHKPSNKGSDGNSMVSLFIKYVCNKLVEVYGIECSVKNVLNLDSSTEEKFSRHLIFMLQSAAFKDNIHVGMSPQSPDAKRQKQEVRDLSFLLVKSKDGRNGLFVDLGVYTKNRNFRLYKSSKVGRNAAFTVAEDNQFTAKPDIEKSAEENVFLASLVCNVSFTGQRILTWDAPETKDVKSPTAQSTFSGCLSSPYKEVDNFVFALVRKDGVQGSIRRWNYFAADQLLVYDIAKYRWCGNVSRFHKSNNIMIIVDLKEEVWYQKCHDPDCRNFRSSSFPLPQEICVSYILTLDEEDQAYLMDDSGNIEPSQAPNSSMHPTEETPEVWGDEQDEREFLKSLNDFEQRSTEIPDELLLTCVADFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAA9</accession>
    <name>A0A3B5LAA9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">USP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAA9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004857.1">
        <property type="protein sequence ID" value="ENSXCOP00000004799.1"/>
        <property type="gene ID" value="ENSXCOG00000003763.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155571"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052398">
        <property type="entry name" value="Ubiquitin_hydrolase_53/54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22975:SF5">
        <property type="entry name" value="INACTIVE UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22975">
        <property type="entry name" value="UBIQUITIN SPECIFIC PROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="3">
        <location>
            <begin position="28"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="287" mass="31959" checksum="CD3A353FD400A1E1" modified="2018-12-05" version="1">DKTIKHENKKSGNGVVAAHTMASIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLSSHKCLEDSCIFCALKNIFAQFQFSSEKVLPSDALRSALAKSFQDQQRFQLGVMDDAAECFENILMRIHFHISDETKEDVCTARHCIPHQKFAMTLFEQCVCSSCGASSDPLPFIQMVHYISTTSLCNQAVKMLESRENPTPSMFGELLRNASMGDLRTCPSRCGQQLRIARVLLNSPEIVSIGLVWDSEHSDLAEDVIHSLGTRLRLGDVRNRPGGEPERGGRMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAF2</accession>
    <name>A0A3B5LAF2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SUMO specific peptidase 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAF2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009178"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009289.1">
        <property type="protein sequence ID" value="ENSXCOP00000009178.1"/>
        <property type="gene ID" value="ENSXCOG00000006999.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155489"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.395.10:FF:000001">
        <property type="entry name" value="Sentrin-specific protease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF30">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="532"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="98"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="313"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="242"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="378"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="744" mass="83556" checksum="D970BC6986BE661F" modified="2018-12-05" version="1">MLNKIYEWIETSFANLRDGEPAVENSPEHPAPTDAPVRRKRPIDCLEDGHSIDQEHLIAKKTRMGDLIDSVRSAAEGVKNHSSNVATWMKKNVNPTLRNILPASSDSPSGEPQPSTSAAVYPRRPIVHRNSLDETFAAPSTILEWKTPKTDWLRNDRMIMGSKVCRRQVCMSPAHREVPKTNGHSVCVRPAIHTKLHPSPRLGRPLNLRPHGASSLSSGVGTANSSCNNMYEKTFPIKVVQSPTHSTSSSRLNKAGSHCTAQESIREEEKEIYRQLLTMVSGGQSTVLHNGSAHTFVRSHRDFTSFLTSHTRLQMSSPAGSAAEGTSEGPSSPPSPRGMSSQSSSNLPSPVGASRKSGSQIWPADTDLSFSQTTALFSAPSPSTLQDNTSQGTESSAHDGDSVIIVNEQKCKKQESSSIPLFQAELWIKELTSMYDSRARERRRQIEEQEALAAQLLLQRLSDEGKRSPDVEVHVRVPLEKEIPLTFVIEKPKSVEEKPEFPELTEEMEAEVHRALMKGGNPHEVLSEGFGLSLTRKDLQTLSNLNWLNDEVINFYMNLLVERSKEPNRPSVNTFNTFFYPKLRSSGYSAVRRWTKKMDIFAKDILLVPVHLGMHWCLSVVDFRKKSVIYFDSMGGSNDQACQILFDYLQQESRDKKGKELDTSGWTLHSKNIEVSDKLNQMFPLDVLPCLQEIPQQMNGSDCGMFTCKYADYITKDKPITFTQKHMPYFRRRMVWEIMNHKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBP2</accession>
    <name>A0A3B5LBP2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Zinc finger protein 335</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBP2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008262"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008360.1">
        <property type="protein sequence ID" value="ENSXCOP00000008262.1"/>
        <property type="gene ID" value="ENSXCOG00000006338.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158508"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050769">
        <property type="term" value="P:positive regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004444">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003059">
        <property type="entry name" value="Zinc finger protein 335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050688">
        <property type="entry name" value="Zinc_finger/UBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24403:SF36">
        <property type="entry name" value="ZINC FINGER PROTEIN 335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="1135"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="433"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="463"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="491"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="529"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="579"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="607"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="880"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="908"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="936"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="964"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="172"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="268"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="11"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="214"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="283"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="319"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="336"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="351"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="381"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1191" mass="133118" checksum="2C8F8F73BAA59AB2" modified="2018-12-05" version="1">MDSEENEVESSSDAGPSGMEEPSESGMGMESSEAMSADSSDAAASHAQAPESDCHVGQSSEGLVVRFSECPLLEDNLNLINVSSVSTVTQSVLVSESAQVLVHSSAVSDGAMMVSDSTASTSSDLGSAIDKIIESTIGPDVMNGCIAVTSAEDGGAETTQYLLLQGPDDGAPMVAQMSSSAMSNRAAIEAHAEGPTSTCQDQGDPQGGLEPDQPDDHPGHSGYREENHQPNLAELEEMMEVVIVHQFKCKMCPYKSVSKDTLINHMRDKHFKPAARQKAEREEQPEEEVDDIVDAGAIDDPEEDSDYNPVDQEIKGRPPAFPRKPPTPISSSSQGRPRRKVGRPRKYNVSGEGNSSKEASSSGLGQGLGREINEDTAEAAISQSDSENKDPSSNSQPEEEFLPRKRGRPSKRFLRKKYRNRYYKSLKPLLRPHNCWICGSRFLTQEDLRFHIDSHEGNDPELFKCLQCSYRCKRWSSLKEHMFNHEGTKPFKCEECDYSSVYKKDVIRHSAVHTCLSFSFLQVPKVLEFPCPVCDKVYPMQKRLTQHMKTHSSEKPHMCDKVWSLFLSNSAFTSQSSVFKCEFCDYSCENKKLLLNHQLSHTNDRPFRCDYCKYSTSKEEFLVSHMAIKHTGEKPFSCDMCHFTTKHRKNLRLHVQCRHPEAFDEWSLAHPEEPVRRRRKPFFTLQQIEELKQQHDDNNPTLQNTIAMQGIENASVTQDALGNTTIIYEHAESSGLSAQNALDLLLNMSNAREIVGNSLQVAVLKSEGSEGEGGTVGALAVAPGQGQKIMTFHVSENGDAVLQEAYEAAASEAGELTHIAIGSYEDAGEFSVVEEPAGEVHSPAYRCLKTEWNTENICFLFLLSLHYSDKSTSSPNTKRFCCRICMESFNGRSDMENHKRAHLDSNTFKCPDCDFTSTAWPEVKSHMGMHSYLRPHKCPSCSFASKNKKDLRRHMMTHTNEKPFACKYCGQRFNRNGHLKFHMERLHSQDHPTRKSRSNASQQTIIVNSDEEALATLQSLQAHQTTITPERLQVLGQDHIIVSQEQPLSDQEEGSYIQQITTIDGQTVQHLMTGDNQVQYIISQDGVEHLIPQEYVVVSDGNQIQVIGQNYFKIYNKFCELLSRFSPSFLNNLKWDAIFLIIIINITFLLAAVADAAMGQTQTLYTEATPEQLEQLQQQGIPYDVITFTNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBQ6</accession>
    <name>A0A3B5LBQ6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="6">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBQ6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005304"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005365.1">
        <property type="protein sequence ID" value="ENSXCOP00000005304.1"/>
        <property type="gene ID" value="ENSXCOG00000004155.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168767"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="7">
        <location>
            <begin position="114"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="221" mass="25606" checksum="AF6888DFF3E0C5E2" modified="2018-12-05" version="1">MDEKAFTKELDQWIEQLNECRQLSEGQVKTLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFKIGGKSPDTNYLFMGDYVDRGYYSVETVSLLVALKVRFRERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALVDSQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGEPHVTRRTPDYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LC43</accession>
    <name>A0A3B5LC43_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Coiled-coil domain-containing protein 39</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Required for assembly of dynein regulatory complex (DRC) and inner dynein arm (IDA) complexes, which are responsible for ciliary beat regulation, thereby playing a central role in motility in cilia and flagella. Probably acts together with CCDC40 to form a molecular ruler that determines the 96 nanometer (nm) repeat length and arrangements of components in cilia and flagella. Not required for outer dynein arm complexes assembly.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC39 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LC43"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009748"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009862.1">
        <property type="protein sequence ID" value="ENSXCOP00000009748.1"/>
        <property type="gene ID" value="ENSXCOG00000007410.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015010"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060285">
        <property type="term" value="P:cilium-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060287">
        <property type="term" value="P:epithelial cilium movement involved in determination of left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036159">
        <property type="term" value="P:inner dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033290">
        <property type="entry name" value="CCDC39"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18962">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18962:SF0">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24161">
        <property type="entry name" value="CCDC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="679"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="273"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="374"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="684"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="703" mass="82005" checksum="64D49CF9CB47D4A0" modified="2018-12-05" version="1">MASRDFDKFSDPEFDKILYEIGWDYSFVLPELNDANKALMEEVRQSRTVHKTPSSYLCTRIMLSHCLFDCVQALMKANEKELEVTKHLKTLAERERSRYCQLTAQAEREISQLKATQNLEELRLQMDLDQQTMENLLAEAAKKDEDLMVIMKYTQMDEHKIKSLTLTIEKKTLEVKEKRKALDKELTETRSTQQQDLMEELFRQRQSFQSAKVKEKNLIALASKNKFTITNLLSQLTKMENTLIRQQMINNNQVLMLQRKVAQLSGASHTNESEMLEVKVKELTANLEDKKETTKILKNALNECEVCLKKHFLTDFPLKFQWSYHILHMASVSLADRYPLCEERDGEDGLVNNNLLKTEVKRVRDIIYIDADSMMSWEMRKLELEKAMKEREEEIHVYSEMLRHQLKFTELDRQKLSMELNEELSKVEMVKNRFEIMESSLAGSEGEKSQIYYITKATLDKEELRRKSEDMKDHIQKMELETKALENTIHLFSGRCSDYSNGLSEMKKFSEEYQEKMQLEERLKTVEETLKLKKRQIQELRQEIQSLMLEEKVEKEKTRHVQGLIANLNKEAASVKERIGRFTKLTREVRSAQSTRSETLEEQDIKLKELKQFNKSINNMLSKAMEEDPDLRPVLEERFQKVVFLMLPRSSASSAGSSHSKSLPWVKTVNLNLDLSAAYPTITSPKSPSSPSSSDSSSSKKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCG1</accession>
    <name>A0A3B5LCG1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCG1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005554"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005620.1">
        <property type="protein sequence ID" value="ENSXCOP00000005554.1"/>
        <property type="gene ID" value="ENSXCOG00000004329.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160080"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000057">
        <property type="entry name" value="neural cell adhesion molecule L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000367">
        <property type="entry name" value="Neural cell adhesion molecule L1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000028">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000038">
        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026966">
        <property type="entry name" value="Neurofascin/L1/NrCAM_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF45">
        <property type="entry name" value="NEURAL CELL ADHESION MOLECULE L1-LIKE PROTEIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13882">
        <property type="entry name" value="Bravo_FIGEY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017181975" evidence="13">
        <location>
            <begin position="25"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="967"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="28"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="125"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="256"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="330"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="392"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="503"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="577"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="677"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="775"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="658"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1041"/>
            <end position="1060"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1088" mass="122557" checksum="C9258C1F6A3E1DD8" modified="2018-12-05" version="1">LRIMRRPGDLLLVLLWTFTSRARMEQPPTITNHTAGPIIALPFDNTLTIRCEARGNPPPAYTWTKDGQELNLPNIPTVKTDNRNGTFMFHNKHFAQFRGKYRCFASNKLGTAMTEEIEIVLSTSPKFPKETFDTIVVKEGYPITLHCNPPEGVAPRQIYWMTHGLQHIKQDERVSMGTDGNLYFAYALPQDSRKDYGCVAAFQRIRTIVQKTPLSVKVERCRFNQQAKTLTFFLNSLLQKCFCSFSMFLILNRRDLLCHVYAQGKSYPTPKITWMKMGETLTDRTKLDNFKKLLTISAVDEKDQGKYMCMAENSAGKADHYFDVIVEEPPSWRTKPPQNQLSVIGSDVFIKCSVDGKPTPVITWRKNGKILQASFLSHYNLSSLNVLLDMAPLMLTKDFQEYAVIEGEDIVMNCSVFSSPPSSVSWTKEGTVVEGERFHVLENGQFLKIIRSEKGDMGKYACMASNREGMSNLTALLDVKGMSAKSLHSSFVNEPKKANQGIGKLARLMCRSEYDRSLQGTFEVIWRKDGEEIFRSSENSFRDLNISDQGRYTCIARTSLDEDTATAFLTVLDTPDAPMSLKISDQESRNVTLSWIPGSDHNSSVREFVVEYEESHWEPGRWKELQKVAGNQATAELFLHGDLNYQFRVYAVNAIGPGPPSEPTERLKTPPAAPDRNPENIRIHGHFPHEMDISWDPLLPIEFNGPGLEYKLSYRKLEVEDKWQEHLVTRPSFIVRNTSTFAPYEIKIQSRNSLGWGPEPRPITGYSGEDIPTAAPHNVAVEVINTTVLRVSWSPVLPATVRGHLGGYNVNWERMKSFLSPDKILEEHKSKSFPGNRTHVIVPGFEPFSEYRLTVNVFNKKGKGPKSDPVNFYTPEGVPEKVPVLIPRIQDDDTVLLEWGPPSVTNGILTGYFLKYHLCMYIHKTHCLIIDVQAFNFLTYIVDLCISLFFLLAEKVGTEASTSTQRWFIGTLCAVALLTLLAVIVCFVMKTKGGKYMFWLFQCDTDPDAPSFVTGDSGSDEKRLKKPILGSTDDTIEDRISRDSLADYSDGDGEFSEDGSFIGEYAGARYRDSISEPSGASGPSTATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDD4</accession>
    <name>A0A3B5LDD4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDD4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010302.1">
        <property type="protein sequence ID" value="ENSXCOP00000010183.1"/>
        <property type="gene ID" value="ENSXCOG00000007707.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161814"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017316842" description="C-type lectin domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="3">
        <location>
            <begin position="38"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="158" mass="18501" checksum="336907DDC38F17F7" modified="2018-12-05" version="1">MAVLSIYKLFLILCLCLCSALNGTVRRSGSCSPGWTRVKGRCYHVFPFGFSLSPLQENCRSIQAHLASIHDYEVNNQLVRMIMSAGRGHTAVWIGGNDIIWERHWRWVDGSPFNYSNWCRYEPNNFFDQDCLQINYRHRCWDDAQCNFRRPFICVKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDF2</accession>
    <name>A0A3B5LDF2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDF2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008691.1">
        <property type="protein sequence ID" value="ENSXCOP00000008585.1"/>
        <property type="gene ID" value="ENSXCOG00000006573.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000263473"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
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        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784:SF3">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 4 MEMBER K-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="32"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="140"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="257" mass="30264" checksum="919208CE3A8F71AF" modified="2018-12-05" version="1">MTTKPSTSLKYSQPVMCFFSAFSTDTGRNQKYHLIQDKKSWQKAQIYCRVNHTDLISGMKQLQDARAANVLNSVGKETHVYFGLFRDAWRWSDGSSLSFRHWHKGFSNQKPNSGQCAVFKFNDKGRWRNESCTAKKHFVCYDDNVILIKENKTWEDALTYCRDHHHDLVTITNMDDQRWMQEKVKNASTDYVWTGLRYTCTLDLWFWVSDEVVIYKNWAKGELMDDCDMSGAMETGGRHQWRKKNDSETFNFFCSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFG5</accession>
    <name>A0A3B5LFG5_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFG5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010898"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011023.1">
        <property type="protein sequence ID" value="ENSXCOP00000010898.1"/>
        <property type="gene ID" value="ENSXCOG00000008233.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000235360"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="127" mass="13903" checksum="C2F485F9C70B5C28" modified="2018-12-05" version="1">MATLGPESARPPLAQLGSPPSNQPHVGCANLPPNRGLERALEEAAASGVLNLSCRKLKEFPRTAANHDLSDTVEAGTVLSALLFLKSCRRFMQNSSGRGHWLLTVGLEWKSPPLPSFFFFFFFHVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFL9</accession>
    <name>A0A3B5LFL9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">MAD2L1 binding protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="32473.ENSXCOP00000009642"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009756.1">
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        <property type="gene ID" value="ENSXCOG00000007350.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003812"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:regulation of exit from mitosis"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR15681">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15681:SF1">
        <property type="entry name" value="MAD2L1-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06581">
        <property type="entry name" value="p31comet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="253" mass="28594" checksum="C8753D1936124B0F" modified="2018-12-05" version="1">ITASNMESLMHKSNFSPSCLKDTNTEKQDAEVVRKAQEEGCVDVVFPGAVTQDGCYRFVSEILKCILYQRQQLPMTYDQLVYSQKKLQTSKPLHSADLDLRKCHQTLQELEELLQQLEVLFSLSRVPRVLLLMGGSLILPKEMYEVNMEALALAAGDQSLRASSCLRQLFRTLFVADLLSDNNPVRLMPTTVLALAHRECGIGWFRPKLQFKVPTRVKNKIINLSTEGHGAEGADWQDYVWFQAPTAIKGFTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LG51</accession>
    <name>A0A3B5LG51_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 12</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF12 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LG51"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011123"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011251.1">
        <property type="protein sequence ID" value="ENSXCOP00000011123.1"/>
        <property type="gene ID" value="ENSXCOG00000008406.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002144"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="C:transcription factor TFIID complex"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:protein heterodimerization activity"/>
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    </dbReference>
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        <property type="term" value="F:TBP-class protein binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07981">
        <property type="entry name" value="HFD_TAF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000011">
        <property type="entry name" value="Transcription initiation factor TFIID subunit 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037794">
        <property type="entry name" value="TAF12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003228">
        <property type="entry name" value="TFIID_TAF12_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12264">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12264:SF21">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03847">
        <property type="entry name" value="TFIID_20kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 12" evidence="7">
        <location>
            <begin position="72"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="178" mass="20105" checksum="B3FE63C7A59D5EFE" modified="2018-12-05" version="1">MEELQTSATQGRIYWWHICYVLHKSGLYGGLESRSSSLPVATSMANSTPIIVKGLTSGPAGRSSPEGSQVLSKKKLQDLVREIDPNEQLDEDVEEMLLQIADDFIESVVTAACQLARHRKSNTLEVKDVQLHLERQWNMWIPGYGSDEIRPFKKACTTEAHKQTDKKVKLLTLYLRGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGG3</accession>
    <name>A0A3B5LGG3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WH1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGG3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009690"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009804.1">
        <property type="protein sequence ID" value="ENSXCOP00000009690.1"/>
        <property type="gene ID" value="ENSXCOG00000007371.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156765"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="C:filopodium"/>
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        <property type="term" value="C:focal adhesion"/>
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        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005522">
        <property type="term" value="F:profilin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001843">
        <property type="term" value="P:neural tube closure"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030838">
        <property type="term" value="P:positive regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01207">
        <property type="entry name" value="EVH1_Ena_VASP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202:SF12">
        <property type="entry name" value="VASODILATOR-STIMULATED PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00461">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50229">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="119" mass="13258" checksum="7C27E38982C56023" modified="2025-10-08" version="2">MSSLCAVRATVLLYDDSSKRWVPAGSDATHVSRVQIYHNATTNTFRVVGRKLQADQQVVLNCPIVKGMKYNQATATFHQWRDPKQVWGLNFGNKEDAALFANSMTHALELISSYREGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LH09</accession>
    <name>A0A3B5LH09_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LH09"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009880"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009998.1">
        <property type="protein sequence ID" value="ENSXCOP00000009880.1"/>
        <property type="gene ID" value="ENSXCOG00000007494.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000033504"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001129">
        <property type="entry name" value="ras-related protein Rab-44 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="65"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="454"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="183"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="686" mass="78593" checksum="2B4216BF875C9533" modified="2018-12-05" version="1">QDEDSNGGSDRTSAEEDDSSRSRSSDVGQNTILEKTHEFFQMCDTENKGFITRRDMQRLNGELPLSADELENVFDSLDSDGNGFLTLEEFSSGFSAFLFGRRISVDDIMTEKNLSKSVPEVLYQSRWKEGPGAAEDDEEKHFVMLMESLGAGSLLEHPAEVRSLWAQLRRDEPQLLSNFEHFLARVTAQIRDANQEKRDMESALKRKTATHDDEVQHLYEEMEQQIKNEKEKILLQDYDRYLSRSKDLELQLSSKEKDLEQLFQKQRRLECQCQELHSEQHETKVENVKLKQTNDDLARELELVSQELTLAQEQLGLLQEQSARLHEEKEMEIYRLSEGLQRERASLHKQLDLLRFDSIFDCFRSNQLQLHVWCFCSEDEEEPTSTALRQNLNGFYQPSFPAEARSRFQRIISIEEDHLPYLLNNSLAQTALQNCAEVETQLDDEDGVDVMSEPRRRSLPIPEQQSEERQVPSSPRGQPVGKETTINVSEESVPSAPDRLFKVVLVGNSSVGKTSLLQRFCDDRFHPGTCATVGIDYSVKTITVDNSQVALQLWDTAGQERYRSITKQFFRKADGVIVMYDVTTEQSFTAVRQWLTSVKEGTGGEIPIMLLGNKMDKEAEREVQKEVGERLAKDCQMTFFECSACSGANVAESMVHLARILKEQEDQQKEKTVQLISSPSKRRSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LH99</accession>
    <name>A0A3B5LH99_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LH99"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010237"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010360.1">
        <property type="protein sequence ID" value="ENSXCOP00000010237.1"/>
        <property type="gene ID" value="ENSXCOG00000007769.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244890"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001234">
        <property type="term" value="P:negative regulation of apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10428">
        <property type="entry name" value="LFG_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="BAX INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291:SF35">
        <property type="entry name" value="PROTEIN LIFEGUARD 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="220"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="283"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="310" mass="33736" checksum="55A5AA7FA47A9B45" modified="2018-12-05" version="1">MSRSDYPPGYDNSHGPMYPPQGGGYPPPPSYGFPSYGGPQPGQPFAPYPSGPNAPLFPGQPTGYPAAPYPGQPQPAGPPGSGYPAPPPMPPVMPPTIPSGVLSSGKGPFILLSSRTCKAFDFFEIVHILSTTQSSNIVETVAVYFITHIVLVCCKGPRRKFPWNVILLLIFVILIQFLSGCSYYNTKAVFLALGITAVVCIAVTVFCFQTKVDFTKCQGLFCVLGIVMFVTGIITAIVLSFKYIYWLHMLYAAIGAIVFTLFLAYHTQLLIGNRKYSISPEEYVFAALSLYVDIVQIFLFLLQIIGSFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHB4</accession>
    <name>A0A3B5LHB4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Mitochondrial basic amino acids transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Carnitine/acylcarnitine translocase-like</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Mitochondrial carnitine/acylcarnitine carrier protein CACL</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Mitochondrial ornithine transporter 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Solute carrier family 25 member 29</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-arginine(in) = L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:32143"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-histidine(out) + L-arginine(in) = L-histidine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:71063"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-histidine(out) = L-histidine(in)</text>
            <dbReference type="Rhea" id="RHEA:72807"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-homoarginine(in) + L-arginine(out) = L-homoarginine(out) + L-arginine(in)</text>
            <dbReference type="Rhea" id="RHEA:72799"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:143006"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-lysine(in) = L-lysine(out)</text>
            <dbReference type="Rhea" id="RHEA:70935"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-lysine(out) + L-arginine(in) = L-lysine(in) + L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:70827"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-ornithine(in) + L-arginine(out) = L-ornithine(out) + L-arginine(in)</text>
            <dbReference type="Rhea" id="RHEA:34991"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-ornithine(in) = L-ornithine(out)</text>
            <dbReference type="Rhea" id="RHEA:71199"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>N(omega)-methyl-L-arginine(in) + L-arginine(out) = N(omega)-methyl-L-arginine(out) + L-arginine(in)</text>
            <dbReference type="Rhea" id="RHEA:72803"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:114953"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 26">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHB4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010098.1">
        <property type="protein sequence ID" value="ENSXCOP00000009980.1"/>
        <property type="gene ID" value="ENSXCOG00000007565.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157766"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005289">
        <property type="term" value="F:high-affinity L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000037">
        <property type="entry name" value="Solute carrier family 25 member 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF61">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="10 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 25 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 26" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="25">
        <location>
            <begin position="2"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="25">
        <location>
            <begin position="89"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="25">
        <location>
            <begin position="184"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00071763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00076491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00078745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00079387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00080567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="282" mass="30888" checksum="E4E3D6635F4EB207" modified="2018-12-05" version="1">KMMDFVAGCIGGAAGVLVGHPFDTVKVRSVQKFDNPLYRGTFHCFKSIVRQESAFGLCKGIGSPMIGLTFISGIVFGVQGNAMRLLGEDTPTHQFITGAAAGTMQSVICCPMELAKTRMQMQGTWQKKWSSKNLYKNSLDCLLHIYRREGMVTTLVREAPGFGVYFWTYAVLTQSLGCEPSDSYMIPKLLFSGGMAGITSWIVIYPVDVIKSRLQADGVGGVNQYSSIADCVRQSVRKEGYMVFTRGLTSTVLRAFPMNAAIFATNSLVLMYARGEEEGPED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJJ3</accession>
    <name>A0A3B5LJJ3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Calsequestrin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Sarcoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the calsequestrin family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSXCOT00000008142.1">
        <property type="protein sequence ID" value="ENSXCOP00000008044.1"/>
        <property type="gene ID" value="ENSXCOG00000006166.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000019377"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033018">
        <property type="term" value="C:sarcoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014809">
        <property type="term" value="P:regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03074">
        <property type="entry name" value="PDI_b'_Calsequestrin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03066">
        <property type="entry name" value="PDI_b_Calsequestrin_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000031">
        <property type="entry name" value="Calsequestrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000047">
        <property type="entry name" value="Calsequestrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001393">
        <property type="entry name" value="Calsequestrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041860">
        <property type="entry name" value="Calsequestrin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041858">
        <property type="entry name" value="Calsequestrin_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10033">
        <property type="entry name" value="CALSEQUESTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10033:SF14">
        <property type="entry name" value="CALSEQUESTRIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01216">
        <property type="entry name" value="Calsequestrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00312">
        <property type="entry name" value="CALSEQUESTRN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017433029" description="Calsequestrin" evidence="8">
        <location>
            <begin position="22"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="370"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="375"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="455" mass="52660" checksum="2A008FC2FB97B837" modified="2018-12-05" version="1">MKWTWVFLGSYFLSLGAPSLGEKGLEIPEYDGKDRVHTLTAKNYKSIMRKYDVMVIYYHKNVDGNRSAMKQLQIEELALELAAQVLDEFEDEDIGFGLVDEKKASAVAKKLDSIYIFADNEIIEYDGELAADTIVEFLYDLLQVIEDPVEIIDNDRELKGFYNMDDVIRLVGYFKSERSPFIEYDDAAEEFHPFVKFFATFDPKFQIAKKLKLKVNEVDFYEPFMGEPVTIPGKPYIESELIEYIEQHDPTLRKLQPHSMYEIWEDDINGEHIVAFAEEDDPGFEFLEILKEVARDNTDDPNLSIIWIDPDNFPLLVPYWERTFGIDLSSPQIGVVDVEDQADSVWMDMDDQDDMPTAEELEQWIEDVLSGKIDPDDDDDDEEDDDDDDDDDDDEEDEDDDDDDDDDDDDDDDDDDDDDDDDDNDDDEDDDXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKB5</accession>
    <name>A0A3B5LKB5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SOWAHA-C winged helix-turn-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the SOWAH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKB5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011357"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011488.1">
        <property type="protein sequence ID" value="ENSXCOP00000011357.1"/>
        <property type="gene ID" value="ENSXCOG00000008585.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183003"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058889">
        <property type="entry name" value="WHD_SOWAHA-C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14491:SF2">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN SOWAHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14491">
        <property type="entry name" value="SOSONDOWAH, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25877">
        <property type="entry name" value="WHD_SOWAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SOWAHA-C winged helix-turn-helix" evidence="6">
        <location>
            <begin position="3"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="319"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="419" mass="46115" checksum="A9317DD494EBEDC4" modified="2018-12-05" version="1">MALTQEAVLCFLLERGGKVKNSELVNHFKSLINSGDPAGKQHNRELFKKLVNSVAVVRQIDEVKFVVVRKRYQDFVKEEMDQSLFRQTANSNARRSSSVSNYYSDDKSCVEGLQGSGPAVSTADSATVKVLNIAGDQPCGASKSGAVFAVIAVRSPERDSAAGARDGSRSQVHQQHGNPDKAVIRVPSLPALTSNSPSHQRELIKELQCWKSKQTEAKQAPGSPLVRPQNKTLRQADDESVPLEPIAHVWLVKCAAGLWGQIYALLLQDTRLAQRKDFMSGFTALHWAAKDGSCDIIHKLIDVSSQRGTYVNVNSKAHGGYTPLHIAAMHGHSEVMVALVQLYGASVNERDHDGKKALHYLGKGASAEVRALLGGQQQSKEKMEGEEYKEHTRGFNTISKLFHPHLGKKHKTCRFAHEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKL3</accession>
    <name>A0A3B5LKL3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 19">Polyprenal reductase</fullName>
            <ecNumber evidence="3 19">1.3.1.22</ecNumber>
            <ecNumber evidence="4 19">1.3.1.94</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Plays a key role in early steps of protein N-linked glycosylation by being involved in the conversion of polyprenol into dolichol. Acts as a polyprenal reductase that mediates the reduction of polyprenal into dolichal in a NADP-dependent mechanism. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>17beta-hydroxy-5alpha-androstan-3-one + NADP(+) = testosterone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50820"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16330"/>
            <dbReference type="ChEBI" id="CHEBI:17347"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.22"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:50822"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 3-oxo-5alpha-steroid + NADP(+) = a 3-oxo-Delta(4)-steroid + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54384"/>
            <dbReference type="ChEBI" id="CHEBI:13601"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:47909"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.22"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:54386"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>a di-trans,poly-cis-dolichal + NADP(+) = a di-trans,poly-cis-polyprenal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80727"/>
            <dbReference type="Rhea" id="RHEA-COMP:19536"/>
            <dbReference type="Rhea" id="RHEA-COMP:19537"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:231623"/>
            <dbReference type="ChEBI" id="CHEBI:231637"/>
            <dbReference type="EC" id="1.3.1.94"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18 19">
            <dbReference type="Rhea" id="RHEA:80729"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>androst-4-ene-3,17-dione + NADPH + H(+) = 5alpha-androstan-3,17-dione + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:50816"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15994"/>
            <dbReference type="ChEBI" id="CHEBI:16422"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:50818"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 19">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 19">Belongs to the steroid 5-alpha reductase family. Polyprenal reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.22" evidence="3 19"/>
    <dbReference type="EC" id="1.3.1.94" evidence="4 19"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKL3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011452"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011584.1">
        <property type="protein sequence ID" value="ENSXCOP00000011452.1"/>
        <property type="gene ID" value="ENSXCOG00000008646.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00500000044920"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047751">
        <property type="term" value="F:3-oxo-5-alpha-steroid 4-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160198">
        <property type="term" value="F:polyprenal reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102389">
        <property type="term" value="F:polyprenol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016095">
        <property type="term" value="P:polyprenol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000021">
        <property type="entry name" value="Polyprenol reductase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039698">
        <property type="entry name" value="Dfg10/SRD5A3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14624">
        <property type="entry name" value="DFG10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14624:SF0">
        <property type="entry name" value="POLYPRENOL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 19" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 19" id="KW-0521">NADP</keyword>
    <keyword evidence="9 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0732">Signal</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017467476" description="Polyprenal reductase" evidence="20">
        <location>
            <begin position="34"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="184"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="243"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="21">
        <location>
            <begin position="184"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
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    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="301" mass="34080" checksum="4FDB83FD92133EFB" modified="2018-12-05" version="1">MTWTSLSLSLIDTCWFFLALCFLVAFSVHKASAQLPGEYRKSSLLTLFQDLIRYGKTKQKLHRAQWLRVFDVPKRWFWHFYAISICWNIFLLLFSSLLGVFISCPILADGGLLSAAPQLSTVLVQLLLCVHSLRRLLECLFVSVFSDGAMHLVQYLFGLGYYIVLGLTVLCSDRPGTLLSRSDWICAAGFALFIAASLLQHQSIVLLAGLRKGKSGQVETLAHRVPEGGWFELVSCPHYFAELLIYVSLGFALGGFSFTWWLVVLYVLFNQALAGQLCHDLYVSKYESYPKHRRAFIPFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKQ7</accession>
    <name>A0A3B5LKQ7_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">Myb/SANT-like DNA-binding domain-containing protein</fullName>
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    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
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            <taxon>Xiphophorus</taxon>
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    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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            <begin position="7"/>
            <end position="99"/>
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            <end position="145"/>
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            <begin position="112"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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    <evidence type="ECO:0000313" key="4">
        <source>
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    <sequence length="145" mass="15906" checksum="3A98856DC98AA24D" modified="2018-12-05" version="1">MESGLRPWGEAEVRALLCVWADRSVRERLKSTLRNKCIFQEMARQMQRKFGVVRNWKQCRTKYKNLKYDYKTAKSAHAAAGSGAGGPGRYMKFFEEVEAILSPTGSCSKALSATGSSATPSTKTSSTSTGPFRGARRTKPSGSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LL83</accession>
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    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
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    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 16" id="KW-0863">Zinc-finger</keyword>
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        <location>
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            <begin position="28"/>
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            <begin position="122"/>
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        <location>
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            <begin position="469"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="544"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="733"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="854"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1553"/>
            <end position="1565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1697"/>
            <end position="1722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1735"/>
            <end position="1752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1766"/>
            <end position="1832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1842"/>
            <end position="1852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1853"/>
            <end position="1862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1862" mass="207812" checksum="29D8584E416E6393" modified="2018-12-05" version="1">MNQSYRPGPRVESAFGPSQPDLRARNGPLNTSYRNQCGTVTRGPGQMPQPAFLGYNQLERFRCYSPQRRPQRLNADPQDRNFHPRSNLHCASSITDEDEGFEPPPIQLPSSPGTDDMDPFESLQNGSRNGFSPRSPDSFERIPDAPIAFWEHRGLHFGSMLFDSSDIKEEEEGHGGVQDLKPFQLPSNATQDRNVTGSKSTCNSVVERRAYKPTVFNLIFNLVKLRYSLSNSPKKKLLPVVKYLDGDLVWAKFNRRPWWPCQVLCDPNQGVHTKMKVPSNRPCRMYFLETVGEVSERTWVPGSVILPFEGGHQFDELPVLRRRGKQKEKDYKYTIPKSLLTAWKVSVAEAEYLLPGRQRNSEKPLEAPSVSIVPNRPDSPGPTTNGNGHHLFKNSTAHGNKSKALKKKKKCLSDIFGHIVGGKETANKAGQLHATTRALKEEPKDSPYADLDSVPMLNRPKRTALSPKYDTDTTDRKEHASVKVKKTSKKLNLCSDPSDLSCIFQNKATSKDPEQRPGKLSQGSSEKHSGTLSASSRLITKALKAAEETNHKDGPATSRVPPEAPREDCFGKTPTDTAITAELSPNRKYPSKTFHNGSVVRSKCEGITRPAQQPVRIKAENSPSDLSTSSSLSLSPMDTFQDSKELRFKSLAMEECSESELSVFRPDSNYKFSTILMLLKDMHDTREKEGKPLVLPPSATLIKEEPLVIPTAAEGGPLRGSCEGLPLQIKLENGQSEKPMLSQSTPVKTNSEKQRRKQRLPSKLKLTTPDLRLDVPERDFISFQSGLTEPVTDSFGPDSSASYLEKSSEIAVAPKKRWQLVEEAAETEAAGSERSAETEAPAEMKGSPTMRASPDLEAHTENDSRSSDARQSENKRLRKPTKRLLESTEEYEQIFSTKKRSKKHTADCSKMVRKVLGVVVSYNSGTSLQERKRPRKLSHKAMECTIEEVSAGSSKKKVCLRNLKLHCLAKVFTLNFFSCLFNNRKPQCLLLGFYRCARNYSYYYDRYIISVPLAACVFLQVCEKTGDLLVCDGPCFGAFHLQCIGLSAAPKGKFLCQECKTGVHNCFVCKKSDNGVKRCIIPLCGKFYHSDCIMAYSPTQPHNKGFRCPLHVCLSCHITNPLNACSTKGRLARCVRCPVAYHPNDNCMAAGSLVLSNNSFLCPNHFAPRKGCKNHEHINVSWCFVCSEGGSLLCCESCPAAFHRECLNIEMPQGSWFCNDCKAGKKPRMKDILWVKWGRYRWWPAEVCLTKDVPNNILRMKHEVGEFPVQFFGSKDFVWTYQARVFPYMEGDTHNIEKMGKGSDAVYKKGINEAASGGQKERQETTSVQVNRPIGKVQIITADLSEIPRCNCKAYDENPCSMDSECINRMLMYECHPQVCAAGERCQNQTFTKRQYTTVEIFRTLSCGWGLRAISDIKKGAFVSEYVGEVIDEEECRARIRHAQENDICNFYMLTLDKDRVIDAGPKGNQARFMNHSCQPNCETQKWTVNGDTRVGLFALQDIPKGVELTFNYNLECRGNGKTACKCGAPNCSGFLGVRPKNQPPAEKMKLKEGKRKGSMKKKTKQQVTKEREDECFSCGDGGQIVSCKKPGCPKVYHADCLNLAKRPAGRWECPWHQCDVCGKEAASFCVMCPNSYCKEHREGKLFISKLDGKLSCSDHDPCGPEPLEPGEVREYMPNMASMRPGNMAVPVPPDTLPAATGATPPSSSSAASLSSTDSQAAPSGREPPPRLYINTKTATSSFVPSSTSYLTDRTELTTGAAREGEDGEVEDGDVYGLDLEEDDEEEDEDDEEDNETEEMEVVEDEEEEEEEPPYGSDALEDEEEEEDEEGGDMCGTWGGYVDDDADDEEVEEWGRLEDDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLG1</accession>
    <name>A0A3B5LLG1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytoplasmic tRNA 2-thiolation protein 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLG1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010391"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010514.1">
        <property type="protein sequence ID" value="ENSXCOP00000010391.1"/>
        <property type="gene ID" value="ENSXCOG00000007871.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001041"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032442">
        <property type="entry name" value="CTU1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00269">
        <property type="entry name" value="TIGR00269 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807">
        <property type="entry name" value="ATPASES OF THE PP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807:SF12">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16503">
        <property type="entry name" value="zn-ribbon_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytoplasmic tRNA 2-thiolation protein 1 C-terminal" evidence="2">
        <location>
            <begin position="131"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="192" mass="21661" checksum="B1CE31DB3027AA84" modified="2018-12-05" version="1">AHPSPIFIVVHLFVCHNADDVAETVLMNILRGDIARLRRCTAISTASEGDGVVPRCKPLKYAYEKEIVLYAYFKKLDYFSTECIYSPNAYRGYARTFLKDLESIRPSSIMDIIHSGENLSVREGVKMPVQGTCSRCGYISSQALCKSCVLLEGLNRGLPKLGIGKHHRLHEKILSQQPLTEREERKLKSVDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLK6</accession>
    <name>A0A3B5LLK6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Splicing factor 3b, subunit 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLK6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011827"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011963.1">
        <property type="protein sequence ID" value="ENSXCOP00000011827.1"/>
        <property type="gene ID" value="ENSXCOG00000008943.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006734"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007180">
        <property type="entry name" value="DUF382"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006568">
        <property type="entry name" value="PSP_pro-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052584">
        <property type="entry name" value="U2_snRNP_Complex_Component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785">
        <property type="entry name" value="SPLICING FACTOR 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785:SF6">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04037">
        <property type="entry name" value="DUF382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04046">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00581">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSP proline-rich" evidence="2">
        <location>
            <begin position="408"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="503"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="653"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="519"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="653"/>
            <end position="676"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="702" mass="80044" checksum="0943F235513499D8" modified="2018-12-05" version="1">MYFSHFPYLSSLQMSVIPPMPPMPMPPMPPGMSMLQPLPMMPPGAPPPPRIPMGLEHAPLPPPGLSQDDQLKMAQQRAAMVLQQEERAKQQVNLTDLSESKPFQLWQNEEISVGGPKIPQVLEKILQLKEIRQEQLTDAPVFFNLHNLNRKKKSKKKRALEKKEQKDKDKDKGKDPEVEIEYVTEEPEIYDPNFIFFKRIFEAFKLTDDVKKEKEKEPEKAEKQEAVGMLRKKGFELERKDSDDSDDDAKPDVPKLSKKKLRRMNRLTVAELKQLVARPDVVEMHDVTAQEPKLLVHLKATRNTVPVPRHWCFKRKYLQGKRGIEKPPFELPEFIRRTGIQEMREALQEKEDAKSMKTKMREKVRPKMGKIDIDYQKLHDAFFKWQIKPKLTIHGDLYYEGKEFETRLKEKKPGDLSDELRIALGMPVGPNAHKVPPPWLIAMQRYGPPPSYPNLKIPGLNSPIPENCTFGYHAGGWGKPPVDEMGKPLYGDVFGTNATDFQSKAEEEEVDHTPWGELEPSDEESSEEEEEEESDEEKPDETGFFTPADSGLITPGGISSVPAGMETPELIELRKKKIEEAMDGNETPQLFTVLPERRTGAVGAAMMASTHIYDMTGVGRKTGGGQESQGVEVALAPEELELDPMAMTQKYEEHVREQQAQVEKEDFSDMVAEHAAKQKQKKRKAQPQDTRGGAKKYKEFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LM89</accession>
    <name>A0A3B5LM89_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Zinc finger and BTB domain containing 47b</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LM89"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013344.1">
        <property type="protein sequence ID" value="ENSXCOP00000013183.1"/>
        <property type="gene ID" value="ENSXCOG00000009992.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160089"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000900">
        <property type="entry name" value="Zinc finger and BTB domain containing 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000312">
        <property type="entry name" value="Zinc finger and BTB domain-containing 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000550">
        <property type="entry name" value="Zinc finger and BTB domain-containing 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000166">
        <property type="entry name" value="Zinc finger and BTB domain-containing 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001300">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000284">
        <property type="entry name" value="Zinc finger protein 652 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="36"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="206"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="284" mass="32994" checksum="4C44EBA3E116D520" modified="2018-12-05" version="1">MNVTHNRMQICNNCGKRFLLESELLLHQQTDCERSIQCVTCGKAFKKLWSLHEHNKIVHGYAEKKFSCEICEKKFYTMAHVRKHMVAHTKDMPFTCETCGKSFKRSMSLKVHSLQHSGEKPFKCESCSERFQYKYQLRSHMSIHIGHKQFMCQWCGKDFNMKQYFDEHMKTHTGEKPYICEICGKSFTSRPNMKRHRRTHTGEKPYPCEVCGQRFRFSNMLKAHREKCFHITNPMVVPNCGDPLGLDLTQLSLSLLHPMGGLATASRLPPPPPLFSAGRMNSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMB6</accession>
    <name>A0A3B5LMB6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Dynein heavy chain hydrolytic ATP-binding dynein motor region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMB6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012107"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012245.1">
        <property type="protein sequence ID" value="ENSXCOP00000012107.1"/>
        <property type="gene ID" value="ENSXCOG00000009151.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158992"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.710:FF:000003">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000543">
        <property type="entry name" value="Dynein axonemal heavy chain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46961:SF18">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46961">
        <property type="entry name" value="DYNEIN HEAVY CHAIN 1, AXONEMAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region" evidence="14">
        <location>
            <begin position="59"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="352" mass="39538" checksum="22B207CE1B2B29B2" modified="2018-12-05" version="1">MRVKLASCGFNDNVILAQKFFVLYKLCEEQLTKQVGTLHVVHNYTFFHDNWHFWDSICLPCQVHYDFGLRNILSVLRTLGASKRARPDDTESSIVMRVLRDMNLSKLVWDEPLFLSLINDLFPGIQLDSSTYVELQAAVSHQVELAGLVNHPPWNLKLVQLYETAQVRHGLMTLGPSGAGKTASINMLQRSLSECGIPHKEMRMNPKAITAPQMFGRLDAATNDWTDGIFSTLWRRSLKAKKAIWIVLDGPVDAIWIENLNSVLDDNKTLTLANGDRIVMSPTCKLVFEVHNMDNASPATVSRVGMVFMSSSALSWEPILQNTLSSSLEAVAYNSIFKIVKCTFSFVQSSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LN54</accession>
    <name>A0A3B5LN54_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LN54"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013690.1">
        <property type="protein sequence ID" value="ENSXCOP00000013523.1"/>
        <property type="gene ID" value="ENSXCOG00000010247.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="108" mass="11389" checksum="BDF80931E6F3C2AE" modified="2018-12-05" version="1">MNPVLTFWTKGSRSSSRTRTSWFTLRTCRVIIKSALPALPARSQLVLNKCDLVQPLSPFSPTPGGPDSPRGPIGPSRPLCPDCPSGPGSPGQKQGDSHPECPICPGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNR3</accession>
    <name>A0A3B5LNR3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 22">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNR3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009484"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009596.1">
        <property type="protein sequence ID" value="ENSXCOP00000009484.1"/>
        <property type="gene ID" value="ENSXCOG00000007220.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157904"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030336">
        <property type="term" value="P:negative regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06609">
        <property type="entry name" value="STKc_MST3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000411">
        <property type="entry name" value="Probable Ste20-like kinase Don3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000092">
        <property type="entry name" value="Serine/threonine-protein kinase 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046409">
        <property type="entry name" value="PDC10_dimerisation_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF7">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="3"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="276"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="339"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="318"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="389" mass="43826" checksum="B2B070BBF38EE0FA" modified="2018-12-05" version="1">ALFTKLDRIGKGSFGEVFKGIDNQTQKVVAIKTIDLEEAEDEIEDIQQEITVLSQCDSPYITKYFGSFLKGSKLWIIMEYLGGGSALDLLKAGPFDEAQIATMLKEILKGLDYLHSEKKIHRDIKAANVLLSEHGEVKLADFGVAGQLTDTQIKRETFVGTPFWMAPEVIQQSAYDSKADIWSLGITAIELAKGEPPNSDMHPMRVLFHIPKSPPPTLTGDFSKSFKDFTESCLNKDPSFRPTAKELLKHKFIVKHCKKTSYLSELIDRYRRWREEGHSDSSSDDSDSESSNKENNESPAWSFTTVRKKKPEDRKVLNGTTQDQLSKPSSLSTLISPIFSELKLQHKEHSEQRQAIEELERNIQVAEKVCPGITDRMITHIIDVSPLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQ93</accession>
    <name>A0A3B5LQ93_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQ93"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013212"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013373.1">
        <property type="protein sequence ID" value="ENSXCOP00000013212.1"/>
        <property type="gene ID" value="ENSXCOG00000010001.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156812"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045117">
        <property type="entry name" value="ATXN2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009818">
        <property type="entry name" value="PAM2_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12854">
        <property type="entry name" value="ATAXIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12854:SF7">
        <property type="entry name" value="ATAXIN-2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07145">
        <property type="entry name" value="PAM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="474"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="417"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="441"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="585" mass="62127" checksum="A2E19AEF96332BE1" modified="2018-12-05" version="1">MAPIETVTTVTSASSPTAASPAPNMVASPSGDGKECRVQETRQSSPSANNENIKSLDNSPSISRPVCKGPPSMAPDHRKQIDNLKKFSVDFRVSILVKLNKNSTGHKPKDLSLDKAAAAGRDGAEDGAAGLTAGGSGAAPASSASKPGSPAAPSPSSLEQKRGGQDVTSQGFQTTAMSTLSGPKHEEKEEKKEAVQDQVRKSTLNPNANEFKPRFNTQPKPANTPTPPRPQGQPSPSIVVQQPPAVYGCFPQMYPLTPVSPGVQVRCTPVQMPHMAVSQSKPYRPVPNMPQQRSDQHHPPGTPTMMHPATAAGPPIVAPNPAYSAQYFTCSPQQFTSQPLVQQMTHYQSQAQHVFSPVMQSTGRMMGPPTHGQPSLVSSSTTQYPEQTHTMYVSPGPMPQQYPHPSATLHPHPQHPQPSATPTGQPQQGGPPQHGGPPSHPAASPVQHPQHQQAAALHLSNAPPQQQIYSALAQTPPSMTPNPQSPQASFPSAQQTVYIHPQVQHGYNPNHMAHMQQAHMQSGIVPSHHPAATHAPMMLMATQGPPGGPQPPMPQAALNPIPVSSTTHFSYLAHAQVQPHHQQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQI6</accession>
    <name>A0A3B5LQI6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Neopterygii</taxon>
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            <taxon>Atherinomorphae</taxon>
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            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 13 ISOFORM X1"/>
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        <property type="entry name" value="Bet v1-like"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
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        <location>
            <begin position="367"/>
            <end position="535"/>
        </location>
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        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="204"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="321"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="327"/>
            <end position="343"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
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        <source>
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            <dbReference type="PROSITE" id="PS50848"/>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="565" mass="63521" checksum="C30759DAB2658DC4" modified="2018-12-05" version="1">MVRIGGKADIHRKFPSNLSDLELFPINISAVKRDHDFLDRDLVEPLCRRLNTLNKCATMKLDVSHPKKKSDDSDEDDHLAISKRWTFEWSSRRWSRLQDFLLDNTNESSPTGLGEGLRSTVSSESVLTDLSEQEITEISSLHSEDSASAMPDSISMASLSASYQPPRELPHYNSLPIKSSRHGQGGKSKAKEFLRKMELMRTWGPSTMKKSSNRRPQLVINGDEPPVSDSKTSKDQITLNVSPSKETVAPSVQEPACTKPRTASKRSSMYLEDMELPSQGKRPEGQSQFGRNQFRSYENLLIHIPKDHKPGTFPKALSIESLAPSPSDRNNNPQSHSQTSPNENGLGEPLSKPACPGAPRGSRVRLIQNLLKEAKDKSKGWVSRLSSDNTELAFKKVGDGNPLRRWRVCLEVSASPSEVLQRLLKERLLWQTELQQEKVLETLDKQTDVYHYTCCNMAPQPCYDYVVLRSWRTDLCKESCALVCVSVEHDDSPHVGAVRGVVLESQYFLEPCGMGKTRLTHISRVDLRGRSPEWYNKAFGHLCVNEAQRIRSSFLPGDQSPEAKN</sequence>
</entry>
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    <accession>A0A3B5LTS4</accession>
    <name>A0A3B5LTS4_9TELE</name>
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        <submittedName>
            <fullName evidence="5">Fas (TNFRSF6) associated factor 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ubiquitin binding"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF96">
        <property type="entry name" value="FAS-ASSOCIATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAF1"/>
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    </dbReference>
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        <property type="entry name" value="UBX"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="4">
        <location>
            <begin position="56"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="UBX" evidence="3">
        <location>
            <begin position="525"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="445"/>
            <end position="515"/>
        </location>
    </feature>
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        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50033"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50053"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    </evidence>
    <sequence length="589" mass="66421" checksum="06B94E3EF7B94730" modified="2018-12-05" version="1">LQAAINGVIPQENGILQSAGPPPSSSSSSSSPPSSSTSAFCPISPSTRHIVERQPRMINFRVEYRQRSVELVLEESRTVGEIKGILQTELGIPASKMHLKGWKSGDVSDTTVLRSLHLPKNNSLYVLTPDIAPTASTSKNSALQDSLNQNFLLVISHREAQRDYSLNFPGSKTIQEVKRNISDLTNIPVRHQQWEGWPASASDDSMTLALSGISYPCHHLSVCRRSSPANAQEPTEECTDVHMISDSEGDDFEDASEFGVDDSEMFGMGTTGCRKSPMMPENSENEADALLHFTAEFSSRYGETHPMFYIGSLEAASQEAFYGKARDRKLLAIYLHNDESVLSNVFCSQMMCADSIVSYLSQNFITWAWDVTKEANKARLLTMCTRHFGSVVTQTIRTYKTDQFPLLLIVMGKRTSNEVLNVIQGNTTVDELMMRLMGAMEIFTAQQQEDIKDEDEREAREMVKREQDEAYRLSLEADRKKREAQEREEAEQVRLEQMRKEQEEEKEAIRLSLEQTLPPEPDEESVEQISKLRIRTPSGEFLERRFLGSCQLQVLFDFVASKGFPFEGFKILTTFPRRNVSILHISLPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LU30</accession>
    <name>A0A3B5LU30_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LU30"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014642"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014828.1">
        <property type="protein sequence ID" value="ENSXCOP00000014642.1"/>
        <property type="gene ID" value="ENSXCOG00000011112.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159326"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13361">
        <property type="entry name" value="PH_PLC_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
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        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037862">
        <property type="entry name" value="PLC-beta_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053945">
        <property type="entry name" value="PLCB1-4-like_EFh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF10">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17787">
        <property type="entry name" value="PH_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22631">
        <property type="entry name" value="PLCB1-4-like_EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLC-beta PH" evidence="1">
        <location>
            <begin position="12"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoinositide phospholipase C beta 1-4-like EF-hand" evidence="2">
        <location>
            <begin position="142"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="225" mass="25951" checksum="42C82B4B257DA546" modified="2018-12-05" version="1">MNKKRYFLDPPEVKDYLVKGERFTKWSETVPVTMKMDPKGFYVYWTNQSKETTFLDVATIRDTRTGKYAKLPKVIRNVFNLDFPDSNHLAKTLTIVSGPDMVNLTYHNFFASKEKVTQNWADGILATAYNAARSNACRQVFLEKIYIRISLQTNKDGKIPVKNIYKMFPADKKRVESALASAHLPKGKVGNQKIPCFLFLNPSKFQSMPNSFFTLFFLTKCDMKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUA5</accession>
    <name>A0A3B5LUA5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytokine receptor-like factor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Cytokine-like factor 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type I cytokine receptor family. Type 3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUA5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012896"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013056.1">
        <property type="protein sequence ID" value="ENSXCOP00000012896.1"/>
        <property type="gene ID" value="ENSXCOG00000009757.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156569"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097058">
        <property type="term" value="C:CRLF-CLCF1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000281">
        <property type="entry name" value="Cytokine receptor like factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000386">
        <property type="entry name" value="Cytokine receptor like factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000690">
        <property type="entry name" value="Cytokine receptor like factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015152">
        <property type="entry name" value="Growth/epo_recpt_lig-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010457">
        <property type="entry name" value="IgC2-like_lig-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF35">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09067">
        <property type="entry name" value="EpoR_lig-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06328">
        <property type="entry name" value="Lep_receptor_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017454274" description="Cytokine receptor-like factor 1" evidence="18">
        <location>
            <begin position="45"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="39"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="139"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="20">
        <location>
            <begin position="240"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="433" mass="48124" checksum="13B231EA0DC23AF8" modified="2018-12-05" version="1">MLPPNRSIRGSLERGAESRPSTRTELPGMVLLLLLLLLPGLDVAVISPQDPVLRIGSGLTASCTLSPELGLHASSLYWTLNGVRLSSSTYSAVSSDTLSIELRSLNGSQQKSGDNLMCHGADGRILAGSCLHVGMPPEKPVNLTCWSHNAKDLSCRWSVSGQGETHIPTKYTLKYKLRWHGRENECENYSSEKKGYACRILAKIALFTPYEIWVEATNQLGSAASDIISLDVLDVVTTDPPADVRLSRIGSLDDQLMVRWASPPELKDFLFQAKYQIRYRMEDGADWKAVDNIGNLTSCRLAGLRPGTVYFVQVRCNPVGIFGSRKPGIWSDWSHPAAASTPSRELLQRGSCDPKAGEQNSALRRELKQFFGWVRKHSPGCGSMSIKLYDQWRVWLQKTRKTRNQSACRPRGFVMRQGCRCRRPGSRLSITLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUI6</accession>
    <name>A0A3B5LUI6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000015663"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015857.1">
        <property type="protein sequence ID" value="ENSXCOP00000015663.1"/>
        <property type="gene ID" value="ENSXCOG00000011832.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157983"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046935">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF7">
        <property type="entry name" value="SUPPRESSOR OF CYTOKINE SIGNALING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017227740" evidence="7">
        <location>
            <begin position="23"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="8">
        <location>
            <begin position="15"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="9">
        <location>
            <begin position="61"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="116" mass="13227" checksum="70DA27C0F7D92C35" modified="2018-12-05" version="1">KNVLNVLLVLIGPGWYWGGLTANEAKEILQDSSEGSFLLRDSSQRDFLFTISTMTSAGPTNLRIEFRHGKFKLDSXXXXXXXXLCRISINRSARRVQDLPIPNRLKDYLTDYVYNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVD1</accession>
    <name>A0A3B5LVD1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVD1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011925.1">
        <property type="protein sequence ID" value="ENSXCOP00000011789.1"/>
        <property type="gene ID" value="ENSXCOG00000008910.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000271280"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="198" mass="19929" checksum="8F89D615DA17A9E4" modified="2018-12-05" version="1">MRRGDGAMRRSDGAMRRGDVTDGPRVPSSSPRSPSDSSESSHRAPPKSSPAVAPPESPPPGGTRDSSPLPPGGTRNSSLPPPGGTRDSSPPGTRDSSPLPPGGTRDSSPLPPGGTRDYSPPPPGGTTDSSPPPPGGTRDSSPPPPGGTRDSSPLPPSGTRDSSLLPPGGTRDSSPLPPGAPPHFVFGDDHSQPSVRIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVD9</accession>
    <name>A0A3B5LVD9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA biogenesis protein RRP36</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the 90S pre-ribosome involved in the maturation of rRNAs. Required for early cleavages of the pre-RNAs in the 40S ribosomal subunit maturation pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Associates with 90S and pre-40S pre-ribosomal particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
            <location evidence="1 6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the RRP36 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVD9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014835"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015021.1">
        <property type="protein sequence ID" value="ENSXCOP00000014835.1"/>
        <property type="gene ID" value="ENSXCOG00000011238.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064271"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009292">
        <property type="entry name" value="RRP36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21738">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING PROTEIN 36 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21738:SF0">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING PROTEIN 36 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06102">
        <property type="entry name" value="RRP36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 6" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="336"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="249"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="63"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="368" mass="42933" checksum="EB1080DA8995A760" modified="2018-12-05" version="1">MRRREKQRQEAERKKKKTVSCSEEEDSDVEKKVSLFTLRGGEAEELRLSGHDDEDQNQSGGGAEEDSGGSEEEEVSEGESNDEGDDDDGDEEEELEEEEANAEEDDDDEEELEEEEADAEEDDDDEEEDAEEEGGHTGDGTELQTEEGIRKELSNMPFEDVIKLQQQVGTKAFNEAAFNNKKCRAVNSKKRLNKNRPMEISSKIPPSFLRQVIPAKKSTRRDPRFDSLSGEYKPEIFEKTYKFINDIKHREKEKQLKKTKTDRKKKEKLEFLLKRLNNQERARLIQEQQRERELQFKKQQRERASLGERPFFLKKSEKKKLQLAEKYQELKKSGKLENFLSKKRKRNAGKDRRKLPGKQLHNKKPFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVR4</accession>
    <name>A0A3B5LVR4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SHC (Src homology 2 domain containing) transforming protein 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVR4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013416"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013581.1">
        <property type="protein sequence ID" value="ENSXCOP00000013416.1"/>
        <property type="gene ID" value="ENSXCOG00000010170.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182870"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01209">
        <property type="entry name" value="PTB_Shc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09925">
        <property type="entry name" value="SH2_SHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000005">
        <property type="entry name" value="SHC-transforming protein 1 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051235">
        <property type="entry name" value="CEP152/SHC-Transforming"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006019">
        <property type="entry name" value="PID_Shc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035676">
        <property type="entry name" value="SHC_SH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10337">
        <property type="entry name" value="SHC TRANSFORMING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10337:SF5">
        <property type="entry name" value="SHC-TRANSFORMING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00629">
        <property type="entry name" value="SHCPIDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="PID" evidence="3">
        <location>
            <begin position="16"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="4">
        <location>
            <begin position="291"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="386" mass="43369" checksum="D6F87665DF69242F" modified="2018-12-05" version="1">TRQKTKYESFQDLNLYMGCIEVLKSMRSLDFNTRTQVTREAINRLCEAVPGGKGAWRKKTPNKALQSVMGKSNLRFAGMNIAVNISVDGLGLLVPTTRQDTPDYVAYVAKDPVNQRACHILECSDGLAQSVISTIGQAFELQFKQYLHSPPKTMSSLVWGEDEDASEHDYYNSIPGKEPPVGGVVDSRLRPTGALLGHIHTQPQNGYLCADGQPPGSKDYEEHQYVNTQSLENLESFTHGADGHKGARAHDSPKKDLFDMSDVLVEDQWPSPPRRRAPVAPNEEQLRRETWYHSRMSRRDAEKLLVRDGDFLVRESTTNPGQYVLTGMHCGLPKHLLLVDPEGVVRTKDMLFESISHLITYHLKNELPIVAAESELHLKQAVRRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXI5</accession>
    <name>A0A3B5LXI5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXI5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014051"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014226.1">
        <property type="protein sequence ID" value="ENSXCOP00000014051.1"/>
        <property type="gene ID" value="ENSXCOG00000010635.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155843"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042043">
        <property type="term" value="F:neurexin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08393">
        <property type="entry name" value="C2A_SLP-1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04020">
        <property type="entry name" value="C2B_SLP_1-2-3-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000006">
        <property type="entry name" value="Synaptotagmin-like 5, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043567">
        <property type="entry name" value="SYTL1-5_C2B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45716">
        <property type="entry name" value="BITESIZE, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45716:SF5">
        <property type="entry name" value="SYNAPTOTAGMIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="4">
        <location>
            <begin position="14"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="277" mass="31790" checksum="4A8C16BDABF47E5F" modified="2018-12-05" version="1">GSVMTMYSGDFVEVQGTIQFSINYVQRLREFHIFVAECRDLAAVDPKRSRSDYVKSYLVPDKANLGKRKTSVKKKTLSPTFNEILRYRVRMEYLRTQTLILSVWHHDTFGKNSFLGEVDVELSKWDFDHTQMNYLALKAKVGEIHIWVKECKNLPLIRATIDFVLPDTSRKSRQKTRVLRRTVEPVFNHTMVYDGIGEADLTEACVELTVWDRDRLASNLLGGLRLGAGTRSYGALVDWMDSGPYEAALWDRMMASPSEWVEDVLPLRMLSSARAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYL1</accession>
    <name>A0A3B5LYL1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Arachidonate--CoA ligase</fullName>
            <ecNumber evidence="13">6.2.1.15</ecNumber>
            <ecNumber evidence="14">6.2.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Long-chain-fatty-acid--CoA ligase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + ATP + CoA = (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19713"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19714"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(9Z)-octadecenoate(out) = (9Z)-octadecenoate(in)</text>
            <dbReference type="Rhea" id="RHEA:33655"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(9Z,12Z)-octadecadienoate(out) = (9Z,12Z)-octadecadienoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45264"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a fatty acid(in) = a fatty acid(out)</text>
            <dbReference type="Rhea" id="RHEA:38879"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54536"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58950"/>
            <dbReference type="ChEBI" id="CHEBI:138261"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:54537"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>hexadecanoate(out) = hexadecanoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45256"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>tetracosanoate + ATP + CoA = tetracosanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:33639"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:31014"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:33640"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.15" evidence="13"/>
    <dbReference type="EC" id="6.2.1.3" evidence="14"/>
    <dbReference type="RefSeq" id="XP_027898974.1">
        <property type="nucleotide sequence ID" value="XM_028043173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYL1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014431"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014615.1">
        <property type="protein sequence ID" value="ENSXCOP00000014431.1"/>
        <property type="gene ID" value="ENSXCOG00000010942.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114160531"/>
    <dbReference type="KEGG" id="xco:114160531"/>
    <dbReference type="CTD" id="541410"/>
    <dbReference type="GeneTree" id="ENSGT00940000159323"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047676">
        <property type="term" value="F:arachidonate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090434">
        <property type="term" value="F:oleoyl-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044539">
        <property type="term" value="P:long-chain fatty acid import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001579">
        <property type="term" value="P:medium-chain fatty acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000002">
        <property type="entry name" value="Long-chain fatty acid transport protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000008">
        <property type="entry name" value="Long-chain fatty acid transport protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006134">
        <property type="entry name" value="PRK08279.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107:SF7">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="23">
        <location>
            <begin position="82"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00035842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00035938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00041297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="648" mass="71808" checksum="731AA2D8F2E001C8" modified="2018-12-05" version="1">MHNAASVSASLGSLGLLRLVGVSWSWSLAAGLGVYLGTKSWKYFYIAARTAKRDVNGLYVLLRVKLALWRYMRNGSSILSIFAQTVKRHPNKPALIYEATGETWTFTQLDEISNAVAHWARGQGWVSGDVVALFLESRPLHVALWLGLAKIGVEAALINFSLRCDPLLHCIGVSGSRAIVFGAELADAMLEINSSISQSIVRFCTGDLSVERLTALNARPLDPVLATASRHPPSPCVPPKGMNDRLFYIYTSGTTGLPKAAIVVHSRYYRIAAFGYFAFRMHPDDVIYDCLPLYHSAGNIIGIGQCLIHGLTVVVKRKFSASRFWEDCIKYNCTVVQYIGEICRYLLSQPVRPSEKGHRVRLAVGNGLRPSVWEAFTERFGVGQIGEFYGATECNCSIANMDGKVGACGFNSRILPNVYPIRLVKVNEETMELIRDSRGLCVPCSPGEPGLLVGRINQKDPLRRFDGYANQDATKKKIVQNVFRKNDSAYLSGDVLMMDELGYMYFRDRSGDTFRWRGENVSTTEVEGILSNVLDQTDVAVYGVAVPGVEGKAGMAAIADATGKFDCDSFLQKIQRSLPTYARPVFLRISPQVDTTGTFKIQKTRLQREGFDPRLTTDQIYFLNSRAARYVPVDEELYNSIAEGRMSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYZ0</accession>
    <name>A0A3B5LYZ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CXXC-type zinc finger protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">CpG-binding protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">PHD finger and CXXC domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Transcriptional activator that exhibits a unique DNA binding specificity for CpG unmethylated motifs with a preference for CpGG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYZ0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012994"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013154.1">
        <property type="protein sequence ID" value="ENSXCOP00000012994.1"/>
        <property type="gene ID" value="ENSXCOG00000009834.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166392"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15553">
        <property type="entry name" value="PHD_Cfp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000138">
        <property type="entry name" value="CXXC-type zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022056">
        <property type="entry name" value="CpG-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037869">
        <property type="entry name" value="Spp1/CFP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002857">
        <property type="entry name" value="Znf_CXXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46174">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46174:SF4">
        <property type="entry name" value="CXXC-TYPE ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12269">
        <property type="entry name" value="CpG_bind_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02008">
        <property type="entry name" value="zf-CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51058">
        <property type="entry name" value="ZF_CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="17">
        <location>
            <begin position="24"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="CXXC-type" evidence="18">
        <location>
            <begin position="115"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="82"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="210"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="334"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="227"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="249"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="264"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="559" mass="66023" checksum="873A3EFA06014EAD" modified="2018-12-05" version="1">DSELSDVDTAPGPESSSMEGENAPLYCICRKPDINCFMGCDNCNEWFHGHCINITEKMAKAIQEWYCMSCRENPLLEVKYRSKKSREKEPDSDRTERQYSTPSTPDYRSDRRRGSKVKRSVRMCGECEPCRRTEDCAQCDFCKDMKKFGGPNKIRQKCRFRQCEVRARKMLRVKEEELSLRERRENSHHRRRRYSEDYDSEAELYQQYRAWGSDDDDDDEPAFSPVMRKKAIKVKHVKRREKKIEKKKESRRHKQKQKHRDKSRHSERGDLRDSGGMRQCLGPNCVKGARPNSKYCSEDCGMKLAARIYEILPQRIQQWQQSPCIAEEHGKKQLERIRRDQQNARLRLTDMERRFHELEGIIAKAKQQAVQQDEEVNEGEGDDTDLQIFCVSCGHPINPKVALRHMERCYAKYESQTSFGSMYPTRIEATRLFCDVYNPQSKTYCKRLQVLCPEHSRDPKVPVDEVCGCPLVKNVFEPTGDYCRVSKRKCNKHYNWEKLRRAEVDLERVRVWYKLDELFEQERNVRTAMTNRAGLLALMLHQTIQHDPLTIDLRSNKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZY7</accession>
    <name>A0A3B5LZY7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">S-adenosylmethionine synthase</fullName>
            <ecNumber evidence="12">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="12">Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the AdoMet synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZY7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016737"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016952.1">
        <property type="protein sequence ID" value="ENSXCOP00000016737.1"/>
        <property type="gene ID" value="ENSXCOG00000012617.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183185"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18079">
        <property type="entry name" value="S-AdoMet_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000003">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000004">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000011">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="S-adenosylmethionine synthetase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 12" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="10 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-adenosylmethionine synthetase N-terminal" evidence="14">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase central" evidence="15">
        <location>
            <begin position="66"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase C-terminal" evidence="16">
        <location>
            <begin position="189"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="332" mass="36940" checksum="DB51B0AB175B7411" modified="2018-12-05" version="1">MVLLCGEITSRANVDYQKVVRDAIKHIGYDNSEKGFDYKTCNVLVALEQQSPDIAQGVHIDRREEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNSKMAELRRNGTVPWLRPDSKTQVTVHYDQKDGAVVPKRVHTIVISVQHDEGISLEEQQRVLKEKVIKAVVPAKYLDDKTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAAYAARWVAKSLVKAKLCRRVLVQVSYAIGVAHPLSISLFTYGSSQKLESELLKIVNKNFDLRPGVIVRDLQLKRPIYQQTASYGHFGRPEFSWEVPKELVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0F0</accession>
    <name>A0A3B5M0F0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CaM kinase-like vesicle-associated protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Does not appear to have detectable kinase activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with calmodulin, in the presence of calcium.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027859691.1">
        <property type="nucleotide sequence ID" value="XM_028003890.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027859692.1">
        <property type="nucleotide sequence ID" value="XM_028003891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0F0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013469"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013635.1">
        <property type="protein sequence ID" value="ENSXCOP00000013469.1"/>
        <property type="gene ID" value="ENSXCOG00000010215.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114136102"/>
    <dbReference type="KEGG" id="xco:114136102"/>
    <dbReference type="GeneTree" id="ENSGT00940000156468"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000188">
        <property type="entry name" value="CaM kinase-like vesicle-associated, like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000155">
        <property type="entry name" value="CaM kinase-like vesicle-associated, like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="24"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="344"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="416" mass="46419" checksum="5798B93A383D1813" modified="2018-12-05" version="1">MPFGCLTLGEKKDYNSPTEVTDKYDLGQVVKSEEFCEIFRAKDRNTLKMYTCKKFNKKDGRKVRKAAKNEIMILKMVKHHNILQLVDAFETKKEYFIFLELATGREVFDWILDQGYYSERDTSNVMRQVLEAVAYLHSLKIVHRNLKLENLVYYNRLKHSKIVISDFQLAKLENGLIKDPCGTPEYLAPEVVGRQRYGRPVDCWAIGVIMYILLSGNPPFYDDSDEEDPDSRDKNLFLKILSGDYEFDSPYWDDISDSAKTLVASLMDVDQDQRLTAQEAIAHEWISGNAASDKNIKDGVCAQIEKNFAKAKWKKAVRVTTLMKRLRGPDQSDSVAAGAAAGAAADANTPGGGPAPPAGGSLSMAATLKAALKEKASDTQTATNPAQSLQPGAQQEEQQSRCNGEAPQMVPQRKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0G8</accession>
    <name>A0A3B5M0G8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0G8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016755"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016972.1">
        <property type="protein sequence ID" value="ENSXCOP00000016755.1"/>
        <property type="gene ID" value="ENSXCOG00000012635.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="65" mass="7571" checksum="8050F443B8C6C65C" modified="2018-12-05" version="1">MNAVKPFSGDSENFRAFRFYSLNAIMDPWVFIIFRKSVFRHLCSLLSCRFVRLRTCATNSSAEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0Y2</accession>
    <name>A0A3B5M0Y2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoserine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">O-phosphoserine phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the last irreversible step in the biosynthesis of L-serine from carbohydrates, the dephosphorylation of O-phospho-L-serine to L-serine. L-serine can then be used in protein synthesis, to produce other amino acids, in nucleotide metabolism or in glutathione synthesis, or can be racemized to D-serine, a neuromodulator. May also act on O-phospho-D-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-D-serine + H2O = D-serine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24873"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35247"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58680"/>
            <dbReference type="EC" id="3.1.3.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:24874"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>O-phospho-L-serine + H2O = L-serine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21208"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="EC" id="3.1.3.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:21209"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027855089.1">
        <property type="nucleotide sequence ID" value="XM_027999288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0Y2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016910"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017127.1">
        <property type="protein sequence ID" value="ENSXCOP00000016910.1"/>
        <property type="gene ID" value="ENSXCOG00000012751.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114133405"/>
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        <property type="reaction ID" value="UER00198"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:magnesium ion binding"/>
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        <property type="term" value="P:L-serine biosynthetic process"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="HAD_PSP_eu"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Phosphoserine phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoserine phosphatase, chloroplastic"/>
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        <property type="entry name" value="Phosphoserine phosphatase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HAD superfamily/HAD-like"/>
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        <property type="entry name" value="Phosphoserine phosphatase, domain 2"/>
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    </dbReference>
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        <property type="entry name" value="HAD-like_SerB"/>
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        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
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    <dbReference type="InterPro" id="IPR004469">
        <property type="entry name" value="PSP"/>
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    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00338">
        <property type="entry name" value="serB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344:SF2">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="active site" description="Nucleophile" evidence="15">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015196"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR604469-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="226" mass="25330" checksum="063DB558C9B7FC50" modified="2018-12-05" version="1">MTTIQETKELFRRADAVCFDVDSTVIKEEGIDELAKFCGVGDVVREMTRKAMGGSMTFKKALTERLSIIRCSREQVNKLITDHPPQLTPGIKELVDRLHKRNIKVFLISGGFRCIVEHVASQLNIPLHHVYANRLKFYFNGEYAGFDETQPTAESGGKGKVISMLKEQYGFETVVMIGDGATDLEACPPANAFIGFGGNVVRAQVKERSSWYVTSFDELLKEMEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M1E8</accession>
    <name>A0A3B5M1E8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tachykinin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M1E8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017095"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017315.1">
        <property type="protein sequence ID" value="ENSXCOP00000017095.1"/>
        <property type="gene ID" value="ENSXCOG00000012890.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000176912"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013055">
        <property type="entry name" value="Tachy_Neuro_lke_CS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00267">
        <property type="entry name" value="TACHYKININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0027">Amidation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017179434" evidence="3">
        <location>
            <begin position="23"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="111" mass="12957" checksum="5FFF17A5BF8D547B" modified="2018-12-05" version="1">MESWKIQLVVVTFCVLVQTSKGSTSGAEDERRLSRHWQYEPVESSPVDSLMKRSKALRFYGLMGKRAGTNKRVAFVDLMGRSVSSEGEQLLKTQVKRKVKISRQLYWWRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2W1</accession>
    <name>A0A3B5M2W1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Potassium voltage-gated channel subfamily D member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">KCND3</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Dendrite</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M2W1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018443"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018675.1">
        <property type="protein sequence ID" value="ENSXCOP00000018443.1"/>
        <property type="gene ID" value="ENSXCOG00000013884.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155343"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005250">
        <property type="term" value="F:A-type (transient outward) potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097623">
        <property type="term" value="P:potassium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18419">
        <property type="entry name" value="BTB_POZ_KCND3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000073">
        <property type="entry name" value="Potassium voltage-gated channel subfamily D member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000111">
        <property type="entry name" value="Potassium voltage-gated channel subfamily D member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000016">
        <property type="entry name" value="Potassium voltage-gated channel subfamily D member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000004">
        <property type="entry name" value="Potassium voltage-gated channel subfamily D member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003975">
        <property type="entry name" value="K_chnl_volt-dep_Kv4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004056">
        <property type="entry name" value="K_chnl_volt-dep_Kv4.3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024587">
        <property type="entry name" value="K_chnl_volt-dep_Kv4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021645">
        <property type="entry name" value="Shal-type_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF182">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY D MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11879">
        <property type="entry name" value="DUF3399"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11601">
        <property type="entry name" value="Shal-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01518">
        <property type="entry name" value="KV43CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01497">
        <property type="entry name" value="SHALCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0630">Potassium</keyword>
    <keyword evidence="9" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0732">Signal</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0851">Voltage-gated channel</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017423889" evidence="20">
        <location>
            <begin position="17"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="353"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="381"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="21">
        <location>
            <begin position="38"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="633" mass="71061" checksum="D92C89A3EDF2A492" modified="2018-12-05" version="1">MATGVAAWLPFARAAAIGWMPVANCPMPIAPRDNSRKQDELIILNVSGRRFQTWRTTLDRYPDTLLGSSEKDFFYNEETKEYFFDRDPDAFRSILNFYRTGKLHYPRHECISAYDEELTFFGIIPEIIGDCCYEEYKDRKRENLERLQDDQEENKDLKQPNLNFRETMWRAFENPHTSTLALVFYYVTGFFIAISVITNVVETVPCGTTTNQKDMPCGERYTVAFFCIDTACVMIFTVEYLLRLFAAPSRYRFMRSVMSIIDVVAILPYYIGLVMTNNEDVSGAFVTLRVFRVFRIFKFSRHSQGLRILGYTLKSCASELGFLLFSLTMAIIIFATVMFYAEKGSSSSKFTSIPASFWYTIVTMTTLGYGDMVPKTIAGKIFGSICSLSGVLVIALPVPVIVSNFSRIYHQNQRADKRRAQKKARLARIRIAKSGSANAFLQSKHNGLLNELLELTVRKPPMLFDTETERKKKAFSDVFIKTDAHEFVDEQVYEQNCLETALQTYPSHSSSIASHDSPAVTCCGASGKKNTPLPNANLPLANPVPSHRGPLQELSALHIQSADPLSHTSSRSNLNLRTDESKRINCKSGRITTAIISLPTPPALAPDADGLHGPPIQTTTSSAGTNIVKVSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M318</accession>
    <name>A0A3B5M318_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027898486.1">
        <property type="nucleotide sequence ID" value="XM_028042685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M318"/>
    <dbReference type="SMR" id="A0A3B5M318"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014384"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014568.1">
        <property type="protein sequence ID" value="ENSXCOP00000014384.1"/>
        <property type="gene ID" value="ENSXCOG00000010913.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114160209"/>
    <dbReference type="KEGG" id="xco:114160209"/>
    <dbReference type="CTD" id="767746"/>
    <dbReference type="GeneTree" id="ENSGT00940000154394"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000003">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="12">
        <location>
            <begin position="47"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="13">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="444" mass="49647" checksum="E2A6572CB211E95B" modified="2018-12-05" version="1">MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRSLSVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEGEFEEEGEEEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3I0</accession>
    <name>A0A3B5M3I0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Paraneoplastic antigen Ma-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3I0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015981"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016180.1">
        <property type="protein sequence ID" value="ENSXCOP00000015981.1"/>
        <property type="gene ID" value="ENSXCOG00000012071.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000205106"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048271">
        <property type="entry name" value="PNMA_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20846">
        <property type="entry name" value="PNMA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Paraneoplastic antigen Ma-like N-terminal" evidence="1">
        <location>
            <begin position="6"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="65" mass="7207" checksum="4980FE89C4512690" modified="2018-12-05" version="1">VSKTLMSLLENWCTGEGLDASHALVVKQVPVETTVKHIEETLMTIKALGRVRVRGRVCLTLNHSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5F4</accession>
    <name>A0A3B5M5F4_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">Receptor for retinol uptake STRA6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
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            <taxon>Xiphophorus</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    <dbReference type="AlphaFoldDB" id="A0A3B5M5F4"/>
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    </dbReference>
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            <begin position="606"/>
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            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000261380"/>
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    <sequence length="644" mass="72775" checksum="9BCB8A4FC6C6B3C3" modified="2018-12-05" version="1">MSFEPFQYSYYDYADWYSNPEPTKPPKEVILPCDPTADEKYFHICILSISFVMMLIMAALTRKNKLCQGFARGSSTNFLDQTQQKGLVMAVFGLVFSKLAMLMIAPDPLPFITDTPANIKEYMKIIAIFYYPVLYYPLLVCGTLQRNAGYVFGTLLSFTHFGVLVWQKFDCPKTPEIYKYYALLASLPQLACLAYLCVQFPVLRVKPDITSLLKKKSSAVSSSETDKPSLVERILEVPKNYIYLPEKVFQFPVKLAVLSFVTCVAIYHTALLLVVLVVPTLHIVRAGIDENIAFLLLGFGIILSEDRMEVVRIVTFYTWLLEVCYLCAMTLSCLVSLVMLMRSMVLHRSNLKGLYKGDAYKNYNTQKTISPSRCGVFCWMGLTGYQAAIVCLGMAIQTLVFFICFLFLVFLIIIPIFYGRNIIVFEVAGKSWPVWVTVVLVTVVQHVTAQFFFVKKDSGTRDLNNRESLFLLTYLLFVVNVVVGLIVGIWRIVITALFNIVHFGRIDISLLHRTAESYDPAYRYYTQFLKVEVSQSHPVLKAFCGLLLDVMVEVGRGGQKTRDAEEGAQENRPNKAASSRRIRARWQLLYTLVNNPSLLGSRKHFQTLQTSESVPNGTPNRSSKKEAEKAEAEAAVSCLPPGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5V7</accession>
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        <recommendedName>
            <fullName evidence="3">Phorbol-ester/DAG-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
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            <taxon>Poeciliinae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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            <begin position="19"/>
            <end position="50"/>
        </location>
    </feature>
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            <dbReference type="Ensembl" id="ENSXCOP00000018765.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    <sequence length="50" mass="6158" checksum="2B1BB9B96178F17F" modified="2018-12-05" version="1">MRPRKRQGAVRRRVHQVNGHKFMATYLRQPTYCSHCRDFIWSESYFKTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6L4</accession>
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        <recommendedName>
            <fullName evidence="3">Protein numb homolog</fullName>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Actinopterygii</taxon>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="3">Plays a role in the process of neurogenesis.</text>
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        <subcellularLocation>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="Numb_domain"/>
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        <property type="entry name" value="PTB/PI_dom"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47368:SF5">
        <property type="entry name" value="PROTEIN NUMB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06311">
        <property type="entry name" value="NumbF"/>
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        <property type="entry name" value="Numb/numb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0217">Developmental protein</keyword>
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    <keyword evidence="3" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PID" evidence="5">
        <location>
            <begin position="12"/>
            <end position="141"/>
        </location>
    </feature>
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        <location>
            <begin position="182"/>
            <end position="215"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="552"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="303"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR017607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="612" mass="65933" checksum="2D395E02E8FF0DF9" modified="2018-12-05" version="1">WSSLTSFHLFLQYLGHVEVEESRGMHICEDAVKRLKTDKKFFKGFFVKVSYCFVTLTLSFDHANDRNCFPQDLILDQTIEKVSFCAPDRNFERAFSYICRDGTTRRWICHCFMAIKDSGERLSHAVGCAFAACLERKQKREKECGVTATFDANRTTFTREGSFRVTTATEAAEREEVMRQLQDAKKGAVTNSSVLQSGSSPSPSSSPPLSVGALGPQVIPRRHAPAEALARQGSFRGFPALSQKTSPFKRQLSLRMNELPSTMQRKSDFPIKNTVPEVEGENDSISSLCTQIATAFSGPPEDPFSSAPMPKPASSPQSPVAPVNGTLPAFSIAATAAAAASIPAVLHPALPTRETNPWAANPAGPAAPVLPGKRLSMVAPPTASPAMSAHKRTPSEADRWLEEVTKSVRAPQSKPLAAGGTPPPQPFAAPEHMPMAPVPVVPAVPFMPSLAPSVPMVPPRQTAFHPPAAASYPVSNGLPFPQSSVPVFGITPSQMVANVFGSTTQPQPFPIPATATMPQTGDAQVFNNVSPYVKPPAPTEVHIQPPNGRAAFNGPEGWAASSSQYGQPAPAQQEDAFEAQWAALENRPHQRTTPSPTNPFSTELHKTFEIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7V0</accession>
    <name>A0A3B5M7V0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027899664.1">
        <property type="nucleotide sequence ID" value="XM_028043863.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027899665.1">
        <property type="nucleotide sequence ID" value="XM_028043864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7V0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019570"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019815.1">
        <property type="protein sequence ID" value="ENSXCOP00000019570.1"/>
        <property type="gene ID" value="ENSXCOG00000014708.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114160926"/>
    <dbReference type="KEGG" id="xco:114160926"/>
    <dbReference type="GeneTree" id="ENSGT00940000155961"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047787">
        <property type="term" value="F:Delta4-3-oxosteroid 5beta-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006699">
        <property type="term" value="P:bile acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19109">
        <property type="entry name" value="AKR_AKR1D1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000003">
        <property type="entry name" value="Aldo-keto reductase family 1 member C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044483">
        <property type="entry name" value="AKR1D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="4">
        <location>
            <begin position="21"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="3">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="326" mass="37154" checksum="210B335364A901CB" modified="2018-12-05" version="1">MALTPQKHSIPLSDGNSIPLIGLGTYSNPQNTPKGTALAAVKRAIEVGYRHIDGALIYCNEDEVGQAIREKIADGTVKREDIFYCGKLWNTFHPPELARPALEKTLQELQLDYVDLYIIEMPTAFRPGDTFYPQDENGKCAYHKSNLCATWEAMEACKDAGLAKSIGVSNFNKRQLELILNKPGLKHKPVINQVECHPYFTQPKLREYCREKNIVIVGYSPLGTNRDPSWVNLECPPMLEDKLLTSIAQKYKKTTAQVCLRFNMQRGVVVIPKSFNLDRIKDNFQVFDFSLSEEEIKAIEGLNKNIRFVVLPFWADHPEYPFHEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M962</accession>
    <name>A0A3B5M962_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">VPS37B subunit of ESCRT-I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">VPS37B</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS37 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M962"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020067"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020314.1">
        <property type="protein sequence ID" value="ENSXCOP00000020067.1"/>
        <property type="gene ID" value="ENSXCOG00000015052.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183012"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036258">
        <property type="term" value="P:multivesicular body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043162">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039702">
        <property type="term" value="P:viral budding via host ESCRT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.660:FF:000003">
        <property type="entry name" value="vacuolar protein sorting-associated protein 37B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037202">
        <property type="entry name" value="ESCRT_assembly_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009851">
        <property type="entry name" value="Mod_r"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13678">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13678:SF9">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 37B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07200">
        <property type="entry name" value="Mod_r"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140111">
        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51314">
        <property type="entry name" value="VPS37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VPS37 C-terminal" evidence="10">
        <location>
            <begin position="76"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="182"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="202"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="225"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="238"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="259"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="278" mass="30925" checksum="448B9C21C03AB4A4" modified="2018-12-05" version="1">ITGFSAPFMTFFPKMHFQFLNWSNMLSGFVQEVQQNKEKTLANNRTLAEQNLTLQPQLEHKKEQLTKSYSCLQESFESYQLRKSRLDHKSGATSLDILLALLQAEGAKIEEETENMADSFLDGDMTLDAFVDSYQNKRKLAHLRRVKIEKLQEMVQKGQHLLSQAAAVPSSRLQDVPAVSSLLADGNSSNSSPVAQPRRKPPAPPSQPPPVLSPTPTTTPQSPFFCPPSQYPPIPPRTGQSFPSVSTGYPSPYISQYPPALPQRPPPRLAPQPGFIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9E9</accession>
    <name>A0A3B5M9E9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein 266</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9E9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016389"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016596.1">
        <property type="protein sequence ID" value="ENSXCOP00000016389.1"/>
        <property type="gene ID" value="ENSXCOG00000012380.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156738"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022832">
        <property type="term" value="F:voltage-gated channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042857">
        <property type="entry name" value="TMEM266"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46842">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46842:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="363"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="363"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="381"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="57856" checksum="75385D1A47F902B1" modified="2018-12-05" version="1">FSNSVEGLSDLEIISQPVEDENQRLAPPVQLVSFGYRDLPLAALDLSLAGSQLLSNADEDENREGSNWLKPCCGRRVALWQVCLFSAGFNCVLVACVILVVLFLSLELLIDTKLLQFSNAFQFATIIHWISLIILSLFFSETVFRIVVLGIWDYIENKVEVFDGAVIVLSLAPMVASTVANGPSSPWDAISLIITLRIWRVKRIIDAYVLQVKVEMELEIQQCEKTKAVREEQLERLTQICQEQAFEIRQLRAHLAQQDLDLTAEREAAMQIHHAWGKQGRSFQVVEGLTPGESDDEDAVVRDDMNNYISQYYSEASSDAGASGIGARVITTAAIDVHLPNSTNPIQSNPKLTMQRVGSAVSDASTSISQSSGSFTVRPPSLSSHTPGSSLTDCSSSAAQATSLSSHRCCPASFSGQDPCKDPSAVVQELLSSLSEDSCLAQKGLVVNPINLKLPSPTGSENGSPELDNRINLFNRRNQEERRGRGRGCVLLQTKPLIHLQGSTTEPSLEEKYRLLGPADSPLGHMSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9I2</accession>
    <name>A0A3B5M9I2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9I2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020180"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020426.1">
        <property type="protein sequence ID" value="ENSXCOP00000020180.1"/>
        <property type="gene ID" value="ENSXCOG00000015123.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182955"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000800">
        <property type="entry name" value="ATP-binding cassette sub-family D member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF68">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY D MEMBER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="114"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="481"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="738" mass="82629" checksum="41685341BF13677E" modified="2018-12-05" version="1">MSNILDAASRVKWDRTTAAKRAVLLAAAAYGVKTLYPIIWKQLCGSRKPNRDRRESKAENGSTCSGKAQIDVLDDRKASPGVNAEFFKQILELGKILFPRLVSRELGLLCLHSVALISRTFLSIYVASLDGKIVKTIVEKKPRHFIIQLMKWLLIAIPATFVNSAIRYLECKLALAFRTRLVNHAYRTYFTDQTYYRVSNMDGRLANPDQSLTEDVMMFSQSVAHLYSNLTKPILDVMLTSYTLIQTARARGANASGPTLLAGLVVFATAKVLRACSPRFGKLVAEEAHRKGYLRYVHSRIIANAEEIAFYRGHKVEMCQLQKCYRALADQMNLILSKRLWYIMIEQFLMKYVWSGSGLVMVAVPIITATGFADNGGDRVALTQMMVSERTEAFTTARNLLASGADAIERIMSSYKEVTELAGYTARVHNMFVVFEDVQRGIYKRSSLSATTGTTLKSKPEMHIDGPLEIKGEVIDVDRGIVCENVPIITPNGDVVVSCLNLKVEEGMHLLITGPNGCGKSSLFRILSGLWPVYGGRLHKPSPEHMFYIPQRPYMSMGSLRDQVIYPDSVEDMAARGLTDKDLEAILDIVNLNHIVTREGGWDAELDWKDVLSGGEKQRMGMARMFYHKPKYALLDECTSAVSIDVEGKIFQAAKDAGISLLSITHRPSLWKYHTHLLQFDGEGGWRFEELDSATRLSLTEEKQRLEAQLAGIPEMQNRLNELCKILGDDSVLKTAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MA03</accession>
    <name>A0A3B5MA03_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Dedicator of cytokinesis 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000020377"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020625.1">
        <property type="protein sequence ID" value="ENSXCOP00000020377.1"/>
        <property type="gene ID" value="ENSXCOG00000015239.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157734"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007520">
        <property type="term" value="P:myoblast fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.740:FF:000004">
        <property type="entry name" value="Dedicator of cytokinesis protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.410:FF:000004">
        <property type="entry name" value="Dedicator of cytokinesis protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000044">
        <property type="entry name" value="dedicator of cytokinesis protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.350:FF:000001">
        <property type="entry name" value="dedicator of cytokinesis protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.350">
        <property type="entry name" value="Dedicator of cytokinesis N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032376">
        <property type="entry name" value="DOCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042455">
        <property type="entry name" value="DOCK_N_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046769">
        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056372">
        <property type="entry name" value="TPR_DOCK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653:SF3">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16172">
        <property type="entry name" value="DOCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23554">
        <property type="entry name" value="TPR_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="6"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="C2 DOCK-type" evidence="12">
        <location>
            <begin position="441"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="13">
        <location>
            <begin position="1227"/>
            <end position="1638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1672"/>
            <end position="1707"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1721"/>
            <end position="1749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1807"/>
            <end position="1836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1672"/>
            <end position="1689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1693"/>
            <end position="1707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1729"/>
            <end position="1742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1807"/>
            <end position="1821"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1836" mass="211818" checksum="0E98D0AE4610ED81" modified="2018-12-05" version="1">CNNRIPLFFSPLAIYNYDPRGEQELRLQVGDTVHILEKLEGWYRGYTLRKKAQKGIFPASYIHLKEATVEGIGKQEIIIPADLPLVQEIGATLREWVQIWQELFVANKITLFRSVQQMAYSLIEYRSQIVSGTLPKDDLVELKKKVTAKIDYGNRILGLDLVVRDEAGNTLDPDRTSTVSLFQAHETASRSVDDRIQEEKTRLQNLEMRRQTLFSSVHTYSLLMNLKNFVCNIGEDAELLMSLYDPDQSEFISENFLVRWDSTGMPKEIEKLNNLPALFTDLSSSDLMRQRLFLVCQIIRVGSMELKEGKKQTGGLRRPFGVAVMDITDVARGKIDDEDKQHFIPFQQIAMETYIRQRQIIMSPLVPSRVIGENEPLTAVFNKVIATREVNHKGQGLFVTLKLLPGDLAQVRKDFPHLVDRSAAIVRKMGFPEIILPGNVRNDIYVTLLQGEFDRGKKKTPKNVEVILSVHDDEGNPMEKAIFPGAGYDGITEYKSVIYYQVKQPCWNETVKVTIPIEDVCRCHLRVMFRHRSSQDSRDKSEKPFGMAYVKLMRGDGTTLKDGRHELFVYKVADVKKADDAKVYLSQPATRVAVEQIQRQTGKPFHHSGVIPVTKDSFQIATLTCSTKLTQNVDLLGLLNWRSNSHDLVQILQRLMEVEGGEIVKFLQDTLDALFNIMMETSEKDTYDTLVFDALVFIITLIGDIKFQHFNPVLETYISKHFSATLAYRKLTIVLNYYVGHPEEPSLTERLYTALKALKYLFRFIVQSRVLSFRFPENSEDPDAFSNSIRLLFLSFNTLMDRPLDEGVKIKGAILKYLPSIINDIQTVFDPVELSVLLSKFIESIPDSQFVRQKLGCMCKIIESDLFKQPDCRDVLLPLVNDQLSGQLDDHSSKPDYEACVQLLSTVLDTLDRKDVGPTRVHFQQIMERLLRRVNRTVISMDRTSPLIGHYLACMTAILKQMDDKHYTHYISTFKTRQDIIDFLMETFIMFKDLMGNVFPADWMVMNLVQMQVFLRAMNQYSDVLNRLFLDPVHFELQLWNNYFHLTVAFLTHKTLQLESFSQEKRTKILNKYGDMRKNMGFRIRDMWYNIGPHKMKFIPSMVGPILEVTLVPEPELRKDTIPIFFDMMQCEHNFSSSRTFQMFENELITKLDQEVEGGRGDEQYKVLLEKILLEHCRRHRYLSQSGEELALLLSSLLENLLAYRTITHDESPEHRMSCTVNVLNFYKEKKREDIYIRYLYKLRDLHLDCENYTEAAYTLLLHAELLEWSDKPCAPHLIPRDGEQVWTQQELKERLFQEIIRYLDKGKMWEKAIELGKQLAKMHEIHMFDFMELSELLKKQAKFYEQIMHAMRPQPEYFAVGYYGLGFPSFLRNKMFIYRGKEYTWLEDFSLKLLSQFPNAVRMTSTAPPGDDICNSPGQHIQCFTVKPVLTVPQRFKDKGVPEQILNYYRHNEVDQFQYSRPFRKGEKDPDNEFATMWIERTTYITAYRFPGILKWFEVKSVSVEEISPLMNAIETMEMANEKLSNLVQQQACDRSLSINPLSMMLSGIVDPAVMGGFSNYEKAFFTDTYIQEHPGDRERIGVLKQLIALQIPLLEDGIRLHGEKATEQLKPLHNRLVTCFRDLREKVEKLYGVITLPCSLTEKKKSRVGSVVVPYIMSSTLRRMSTASTLSSTSSGLSSGSSDGASSQESVPDRRVSVLSRSEEDNRIARKNRKEWSMSKSQVLLEKQSDADEVSPDKQQRPKSLQLGDRRLTLSLFQGVSSQLSLSNQLSPLAASPHTPNTPRSSSTYLNTNLFFFSPQFVPPLPVKIENKPPPPPPKTRNKAAPTRRPWEFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MA10</accession>
    <name>A0A3B5MA10_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">GTPase IMAP family member 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Immune-associated nucleotide-binding protein 9</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Exerts an anti-apoptotic effect in the immune system and is involved in responses to infections.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MA10"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016604"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016819.1">
        <property type="protein sequence ID" value="ENSXCOP00000016604.1"/>
        <property type="gene ID" value="ENSXCOG00000012536.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271858"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01852">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000536">
        <property type="entry name" value="GTPase IMAP family member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000366">
        <property type="entry name" value="GTPase, IMAP family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF188">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AIG1-type G" evidence="17">
        <location>
            <begin position="23"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="AIG1-type G" evidence="17">
        <location>
            <begin position="436"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="228"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="231"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="751" mass="83651" checksum="11477AFA8D6C7B1D" modified="2018-12-05" version="1">IFCSEHQRQTADQRTDGDAYHCTKRLRIVLIGKTGSGKSSSGNTILGRKEFKAEASPQSVTTKCDKSYCKVDKHHVVVVDTPGLFDNNLTPGQVNKELKKCFSLVSPGPHVFLLVLQIGRFTKEDTETLELIEEILGENYKKFTIVLFTRGDALEYEKMSIKYYTEHDCDDACKNLIRDCGGRYHVFNNYKIKNRSQVTDLIKKINRMMKDNEGSCYTREMLPVTETKINSPVCSDPGSGPGSDPGSDPGSGPDSDPDSVPVSELRVVLLGGNWSQRSSVGNFILGCNGFNKSLNTFKKISAELEEKQISVINSPDVLFSTAEKLTEFIRKCAEVSDPGPHVFLLVLHSESFTRGEKDKICRILQDFSDRSFDHSLVLILKSRQEGSMIGSPLKDLIRKCRYRNLKMEETEPEQLLIRFEIIFNLRNFNPQQIPAADCLRIVLIGKTGGGKSSSGNTILGRKQFEAKASQQSVTRNCQKAQTVIAGRRVVVVDTPGLFDNTLTADQVDEELLKCISLLAPGPHVFLLVLQVGRFTEEEKRTLRQMKKVFGKNSEDFTLILFTGGDKLQYEEKTIEEYIREGCDESFRKLVNDCGGRFHVFNNYDTKNRSQTNELIQKINNMVKKNKGKCFTSEILNANAWLSLNANACLSLNANACLSLSANAWLSLNANACLSLSANACLSLSANACLSLNANAWLSLNANAWLSPGGCLMVESQWSSGSAAGFHLIRFEDLPSFVQERKKHQRDEGGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MA23</accession>
    <name>A0A3B5MA23_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HotDog ACOT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MA23"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021098"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021357.1">
        <property type="protein sequence ID" value="ENSXCOP00000021098.1"/>
        <property type="gene ID" value="ENSXCOG00000015785.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005330"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03442">
        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655:SF0">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="6">
        <location>
            <begin position="42"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="171" mass="19394" checksum="A08C733E9DE621B4" modified="2018-12-05" version="1">MLGLCLLFLCRHSDHQQAMAERVSLASMLARSQSQLPAKRMKDSFLEVHLPLGSEPALREKYLTNHNTVRFGRILEDLDSLAVLISYSHTYNPALKRSPLSIVTALVDKIDMRQHVIYPDCDIKFTGQVTWVGRSSIEAKMHMSQYRDGAYAPVLDATFVMVARDPGNKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDQ6</accession>
    <name>A0A3B5MDQ6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Olfactomedin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDQ6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022393"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022667.1">
        <property type="protein sequence ID" value="ENSXCOP00000022393.1"/>
        <property type="gene ID" value="ENSXCOG00000016728.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158020"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003112">
        <property type="entry name" value="Olfac-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050605">
        <property type="entry name" value="Olfactomedin-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192:SF85">
        <property type="entry name" value="GLIOMEDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192">
        <property type="entry name" value="OLFACTOMEDIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02191">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00284">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51132">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Olfactomedin-like" evidence="4">
        <location>
            <begin position="47"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="288" mass="33173" checksum="D8469251E516A5A9" modified="2018-12-05" version="1">TLDISEFYSPPSKKLESGIEVLYMCILQEKNWIFPKSHKVFALFYLGCNIKAIKCSDKSINMQLTFGAWMSDASRPDDRIWVAEHFSGHIFRQLKNISDTKQKFIDIEKYYQGCGHVVYKNSFYFHNGGTNNLLKFTLNTKNTSSLEMPNSKYKGLRYLFHNSKTYFKFAVDENGLWVIFASYTDSNTMVAKLDPDAFFVLSIINTGYPSAKAGNAFIVCGVVYFTDNNDKRVTYAFDLMTQSYLDASFDLRAGDGTLAMLSYYPNKRLLYMWDNKSARTCKIKFKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDT3</accession>
    <name>A0A3B5MDT3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Gastrin/cholecystokinin peptide hormone domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the gastrin/cholecystokinin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDT3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021792"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022056.1">
        <property type="protein sequence ID" value="ENSXCOP00000021792.1"/>
        <property type="gene ID" value="ENSXCOG00000016289.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00970000194068"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015499">
        <property type="entry name" value="CCK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001651">
        <property type="entry name" value="Gastrin/CCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013152">
        <property type="entry name" value="Gastrin/cholecystokinin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10786">
        <property type="entry name" value="CHOLECYSTOKININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10786:SF0">
        <property type="entry name" value="CHOLECYSTOKININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00918">
        <property type="entry name" value="Gastrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00029">
        <property type="entry name" value="GASTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00259">
        <property type="entry name" value="GASTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0027">Amidation</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0765">Sulfation</keyword>
    <feature type="domain" description="Gastrin/cholecystokinin peptide hormone" evidence="9">
        <location>
            <begin position="24"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="129" mass="14664" checksum="68AC92DA24414AD6" modified="2018-12-05" version="1">MDLGVCLKFEALIRISVHLPFFCQRAESTSHLTNSLHAPSPSRTRQVRSAPAPPSDRFAHYSQLREHAEAPNSLNQLLARLIPRKGSAFQIRSSLSSRALAPSHRIEDRDYLGWMDFGRRSAEEYEYSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MF85</accession>
    <name>A0A3B5MF85_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MF85"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022250"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022519.1">
        <property type="protein sequence ID" value="ENSXCOP00000022250.1"/>
        <property type="gene ID" value="ENSXCOG00000016618.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000235509"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.780">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5032819973" evidence="1">
        <location>
            <begin position="23"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="75" mass="8619" checksum="B6C70F19EBF692FF" modified="2018-12-05" version="1">VPFFFSFYFFSVLFSIFMETEANYERLRIGGLVFTCLLIIGAVLLLVCNCVRGNRSQKLDETHIKNHIKSLLSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHI7</accession>
    <name>A0A3B5MHI7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. ROCO subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023778"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024062.1">
        <property type="protein sequence ID" value="ENSXCOP00000023778.1"/>
        <property type="gene ID" value="ENSXCOG00000017757.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165193"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000361">
        <property type="entry name" value="Leucine-rich repeat serine/threonine-protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000091">
        <property type="entry name" value="leucine-rich repeat serine/threonine-protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1390">
        <property type="entry name" value="ROC domain from the Parkinson's disease-associated leucine-rich repeat kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032171">
        <property type="entry name" value="COR-A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057263">
        <property type="entry name" value="COR-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050647">
        <property type="entry name" value="Plant_LRR-RLKs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020859">
        <property type="entry name" value="ROC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056">
        <property type="entry name" value="LRR RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48056:SF81">
        <property type="entry name" value="RECEPTOR PROTEIN-TYROSINE KINASE CEPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16095">
        <property type="entry name" value="COR-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25497">
        <property type="entry name" value="COR-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08477">
        <property type="entry name" value="Roc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51424">
        <property type="entry name" value="ROC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Roc" evidence="14">
        <location>
            <begin position="395"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="1012"/>
            <end position="1294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1780" mass="199638" checksum="083EBA5AF9239428" modified="2018-12-05" version="1">MLAGHFLWQAVLRCLIIGHISEIILCGAEYVCLLVSLPYLDLDWFMDVCSQITHLDLSSNSLGTLPSVVPWGLIHLRTLDLSNNALKELPFAQNSQDVICSRLQMVNISQNQLKSLPTGLLHLVHLQKLCVADNQLSTLFDIPDTVNWIGLRKLEGLDVSGNCLTSLLTSVMHCLKSLRCLDVSRNKLSAFPDPWACPLNQCKASSNRIENLPNTISIFWRTQLQEVDFSDNCLKELPSYIFELEAIVSLKLCGNLIEMLPSANKWKCSKLKTLDLSRNQLGRSVHTLTWKHFLVHRQAAKISLFPSVLRDSLEVLILNDNQLECVPQSLCALHNLTELYLSNNPGIRELPAELGQLSNLWQLDIENLNINNIPQAVKDEGTNSVLAFLRAQLRKAEPCRLLKMLVVGPPRQGKSALVEALLTGKASPFTPSECSISTFSWELEKPNAGKNNKESVVFHVWDIGGPASMTTVNQCFFTDKSLYVVIWNLALGEEAVANLQTWLLNIEARAPNSSVVVVGTHLDLIDTKFRTERLATLRAYVLALCRSPSGSRAAGYPDVTVKQLHEVSCKTLEGFDGLKMLLYNVALSMKDSSSSSSSGCGSKLLARLIPRSYLILQEAVIAEKKKRHAEGEVQYLTEAQLLSIIEQNPESDIRDYEDLQTAISFLIETGTLLHFPDTSHGLCTLYFLCPVWLSDCLERIVHLNSCRPVPRNGVIKVEDLRKLLAGTGFTQETEEQYFQFLAKFEIALPVANDRYLLPHLLPPKPAMDIHSFHRHTNNTLQRLFKMSFVPVGFWERFIARMLISLTEMDLQSFDSAKNRSLNRRSSLIYSFAGSQQRNRCSTFRVRRSQTIYWKEGLLVTFDGGYLSVESSDVNWKKMKSGGIKILCQSDIRDFSAMAFITDHVNSLMEQWFPALTGTQSDGSLLIEQYVPCSLCAPRDQQQEQAEDSRRGEAEVHYFNIEDCVLAAVEQEHIICPQHPEQPIPLQELVPELFMTDFPARLFLEKAQLELCEVEQNILGQGGSGTIIYRARYRDQPVAVKLFHFKRCRQLSATSDRDTMVKHLQFVNACRSFSEFRQEASMLHSLQHPCIVALVGISIHPLCFALQLAPLGSLNTVLEERHKGSEYMPLGHMLTFKVAYQVAAGLAYLHRKNIIFCDLKSDNILVWSLEDHINVKLSDYGISRQSFHEGALGVEGTPGYQAPEVRPGIVYDEKVDMFSYGMVLYELLSGRRPALGNHQLQIAKKLSRGVRPVLGSLEQVQFYSLHTLMTECWDTKPEKRPVALWCVRQMEEPSFPCLKYLLSCDSQSQLFLSQLQGCGAVFWHGDNEDRNYSVVNVEKGQVEVKRMCCPGNRISCQMKMPNSLWISTEEQEVFVYSLKDMCPLSQPQKRLSCPAVVTCLFHVPAAEQSLAKVFAGMSDGLVAVYTLVDDLPLDGEMYLCSHTLNKTVFGLKDSDPRQRPYPVRSMALLSSGSQLWFSNGPGLLVIDTSSLQAVRRLEPYKAPSSVVSMTTSFSLCGEEAVWTLDDHSNTLKLYYAASYELCATYCCGDRNPLRDVFAVQRPAGMAAAAGDDINTTDQNGKLEWSNGDVTLIFSEEAGTQIIQHQDSVTDYCSLSSPCSLEPPQSNCLSATDCSSLRSRSCSPLALNLQAQQEPRTTNLYSAEEINPAVPELQAFNLLPVNGTVWIPRRGGDLIIIEIQSCGNQLRGRVSAVLSPPGLSSLGTLEEAALVAKDTVVCGFQQKENMEWCLVVWRAWGHQELDVFYQSWEELYRSESSSRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHP7</accession>
    <name>A0A3B5MHP7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Progestin and adipoQ receptor family member VII, b</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027868867.1">
        <property type="nucleotide sequence ID" value="XM_028013066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHP7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020646"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020897.1">
        <property type="protein sequence ID" value="ENSXCOP00000020646.1"/>
        <property type="gene ID" value="ENSXCOG00000015469.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114142047"/>
    <dbReference type="KEGG" id="xco:114142047"/>
    <dbReference type="CTD" id="368256"/>
    <dbReference type="GeneTree" id="ENSGT00940000161438"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003707">
        <property type="term" value="F:nuclear steroid receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005496">
        <property type="term" value="F:steroid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007193">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001556">
        <property type="term" value="P:oocyte maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF41">
        <property type="entry name" value="MEMBRANE PROGESTIN RECEPTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0896">Oogenesis</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0754">Steroid-binding</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="321"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="352" mass="40823" checksum="6A69435ED759A93B" modified="2018-12-05" version="1">MATVVMEQIGRLFINAQQLRQIPQLLESAFPTLPCTVKISDVPRVFRERHILTGYRQTDQSWRYYFLTLFQRHNETLNVWTHLLAALIILVKCQEISETVDFLRDPHAQPLFIVLLAAFTFLSFSALAHLLSAKSELSYYTFYFLDYVGVSVYQYGSALAHYYYAIEKEWHSSVQGFFLHTAAFLAWLTCFGCCYGKYARHDLPKFAHKLLQVVPSALAYCLDISPVVHRIYSCYWGGCSDPIVWYHVYHVIFFLISAYFFCCPHPESLLPGKCDFFGQGHQIFHVLVVVCTLTQIEALRTDFTERRPLYERLHGDLAHDAVALFIFTTCCCALTAFYVRNRVRASLYEKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MI13</accession>
    <name>A0A3B5MI13_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027857693.1">
        <property type="nucleotide sequence ID" value="XM_028001892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MI13"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023270"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023548.1">
        <property type="protein sequence ID" value="ENSXCOP00000023270.1"/>
        <property type="gene ID" value="ENSXCOG00000017378.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114134989"/>
    <dbReference type="KEGG" id="xco:114134989"/>
    <dbReference type="GeneTree" id="ENSGT00940000154664"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF172">
        <property type="entry name" value="KELCH-LIKE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00501">
        <property type="entry name" value="KELCHREPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="25"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="588" mass="65818" checksum="2BFEDA181151E097" modified="2018-12-05" version="1">MSDKVQHPNEQDSVYNQLRLAGKLTDAVIIAEGVGFPVHKIILCNCTPYFCELFTRSCSLDQEIFHIPGISAEVMGLILDYAYTNSLTITAGNVQQLLLTANLLCAPEVVHACCIFIEDLIHPDNCIGMWRYTTTVCPQPVLHYKIYCYILKHFEAVFLCEEFLLLSAGELTDIIETDNLIVKTERTVYEAILRWTSHALEERQAHFPSLFSKIRLALLDVDYIRDKVLPSTLTQGPCLSLLSEATKTITQLLSIRPYLTGVCNPLARPRLPTGILLAIGGWSGGGPTNGIEAYDVRADCWINVTNNTERPRAYHGTIFLNNFVYCIGGFDRLEHFNSVRKFDPIKGNWHEVAPMYYQRCYVSVTVLNGCIYAIGGYDGHMRLSTAEYYRPELNQWTLIAPMQEQRSDANCTTLNNKIYICGGFNGHDCLQTAEYYSPETNQWTMISPMNSQRSGIGVVAYAGHVYAVGGFDGNSRLRSVEAYNPETNAWTNVASMITTRSNFGIEVVEDRLFVVGGFNGFTTSYHVECYDATRNEWLEVCGMGIFRSAVSCCVIAGLPNMPEYVVPRDALPLLPIEEDHETDSGDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIE8</accession>
    <name>A0A3B5MIE8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 12">Kinetochore protein Spc24</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Chromosome</location>
            <location evidence="12">Centromere</location>
            <location evidence="12">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the SPC24 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIE8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020891"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021148.1">
        <property type="protein sequence ID" value="ENSXCOP00000020891.1"/>
        <property type="gene ID" value="ENSXCOG00000015639.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005584"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.570">
        <property type="entry name" value="Ncd80 complex, Spc24 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013252">
        <property type="entry name" value="Ndc80_Spc24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22142">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22142:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN SPC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08286">
        <property type="entry name" value="Spc24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 12" id="KW-0132">Cell division</keyword>
    <keyword evidence="10 12" id="KW-0137">Centromere</keyword>
    <keyword evidence="3 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 12" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5 12" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="94"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="225" mass="26481" checksum="3A9935B8B3E43C17" modified="2018-12-05" version="1">MCCHLRRTRRYSFCSSPFFPVFQLPTMSLSHKFQDVEENGEMLVAFINSSQPEKLREVKVERQALVDRHLETKKTVTQILKDVAQIEERAGQRLLNMEEQKQHRQKELEDLEEQLQRCTAKSQITDSEIQFLQTELESVRNTERELETLQNEVHEDTTEVIPSAVYVAQVYYLITKIKWEYDMQPNILKGVHYGPDLATPINIDTSVRSRSDISDQLWDFVSTEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIJ6</accession>
    <name>A0A3B5MIJ6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027873549.1">
        <property type="nucleotide sequence ID" value="XM_028017748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIJ6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019379"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019617.1">
        <property type="protein sequence ID" value="ENSXCOP00000019379.1"/>
        <property type="gene ID" value="ENSXCOG00000014563.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114144672"/>
    <dbReference type="KEGG" id="xco:114144672"/>
    <dbReference type="CTD" id="115861"/>
    <dbReference type="GeneTree" id="ENSGT00940000161246"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045494">
        <property type="term" value="P:photoreceptor cell maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029520">
        <property type="entry name" value="RdCVF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47109">
        <property type="entry name" value="NUCLEOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47109:SF1">
        <property type="entry name" value="NUCLEOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13905">
        <property type="entry name" value="Thioredoxin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin-like fold" evidence="2">
        <location>
            <begin position="32"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="231" mass="27187" checksum="67E46AE8C102E75B" modified="2018-12-05" version="1">MVDLFADRVLVKNNKDQDELDTEREIITRLQNRILMLFFASAESDTCQRFAPTLHDFFKQLTDEFYVERSAQLVLLYVSLDQSVEQLENFLKELPKKSLFLAYEDPYRRELETMFDVREVPTVVVLRPDCSILIPNAVEEITRLGPDCYRNWHEAAELIDRNFLTKEDFEDKSMRSFTDPVRRLKYKVEDEKKKKKEEEKKKKKQRRGWGVGGDEGEEAGADDRDGGGSMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJM6</accession>
    <name>A0A3B5MJM6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mothers against decapentaplegic homolog</fullName>
            <shortName evidence="9">MAD homolog</shortName>
            <shortName evidence="9">Mothers against DPP homolog</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">SMAD family member</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the dwarfin/SMAD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJM6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024539"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024835.1">
        <property type="protein sequence ID" value="ENSXCOP00000024539.1"/>
        <property type="gene ID" value="ENSXCOG00000018332.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153499"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071144">
        <property type="term" value="C:heteromeric SMAD protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070411">
        <property type="term" value="F:I-SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032924">
        <property type="term" value="P:activin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009953">
        <property type="term" value="P:dorsal/ventral pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009880">
        <property type="term" value="P:embryonic pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060395">
        <property type="term" value="P:SMAD protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10491">
        <property type="entry name" value="MH1_SMAD_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10985">
        <property type="entry name" value="MH2_SMAD_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.10:FF:000001">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.520.10:FF:000001">
        <property type="entry name" value="Mothers against decapentaplegic homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.520.10">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013790">
        <property type="entry name" value="Dwarfin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003619">
        <property type="entry name" value="MAD_homology1_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013019">
        <property type="entry name" value="MAD_homology_MH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017855">
        <property type="entry name" value="SMAD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001132">
        <property type="entry name" value="SMAD_dom_Dwarfin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036578">
        <property type="entry name" value="SMAD_MH1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703:SF53">
        <property type="entry name" value="MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13703">
        <property type="entry name" value="SMAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03165">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03166">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00523">
        <property type="entry name" value="DWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00524">
        <property type="entry name" value="DWB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56366">
        <property type="entry name" value="SMAD MH1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51075">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51076">
        <property type="entry name" value="MH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MH1" evidence="11">
        <location>
            <begin position="11"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="MH2" evidence="12">
        <location>
            <begin position="234"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="168"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="205"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="427" mass="48409" checksum="9E4912C4DDD02BFF" modified="2018-12-05" version="1">MSMSILPFTPPIVKRLLGWKKGEQNGQEEKWCEKAVKSLVKKLKKTGQLDELEKAITTQNINTKCITIPRSLDGRLQVSHRKGLPHVIYCRLWRWPDLQSHHELRAVELCEYAFHTKKDEVCVNPYHYQRVETPVLPPVLVPRHTEIPSEFPPLDDYSHSIPENTNFPAGIEPQSNYIPETPPPGYLSEDGETSDHQMTHSMDTSSPNLSPNPVSPTHSNLDLHPVTYCEPAFWCSISYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNAAVELTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPNIRCSSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKT1</accession>
    <name>A0A3B5MKT1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Olfactory marker protein b</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKT1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024989"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025293.1">
        <property type="protein sequence ID" value="ENSXCOP00000024989.1"/>
        <property type="gene ID" value="ENSXCOG00000018652.1"/>
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    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
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        <property type="term" value="P:sensory perception of smell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="Gene3D" id="2.60.120.390">
        <property type="entry name" value="Olfactory marker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009103">
        <property type="entry name" value="Olfactory_marker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036727">
        <property type="entry name" value="Olfactory_marker_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15357">
        <property type="entry name" value="OLFACTORY MARKER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15357:SF0">
        <property type="entry name" value="OLFACTORY MARKER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06554">
        <property type="entry name" value="Olfactory_mark"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63697">
        <property type="entry name" value="Olfactory marker protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="161" mass="18535" checksum="0C00108E74AEFD34" modified="2018-12-05" version="1">MGSESPDTELHFRLDSQLTEVMRLRVQSLQQRSQKRQEGERLLRPNEAVYRLDFSKQSLRFSHWMVRLAQPGRLTITATSQLWTPDLTNLMTRQLLEPVGVFWRTAAEDAPAQCYEADAAEFGERIAELAKVRKVMYFLFAFAEGCSHESVDCSITFMVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5ML18</accession>
    <name>A0A3B5ML18_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5ML18"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024443"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024737.1">
        <property type="protein sequence ID" value="ENSXCOP00000024443.1"/>
        <property type="gene ID" value="ENSXCOG00000018255.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001532">
        <property type="entry name" value="Immunoglobulin heavy variable 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="101" mass="11410" checksum="382920C63E40C9E7" modified="2018-12-05" version="1">EVHLTESGSVVKKPGESHKLTCTASGFTFSSYWMNWIRQAPGKGPEWIARISSSGGTKYYSQSVQGRFTISRDNSRDQVFLQMNSLRTEDSAVYHCVRETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5ML76</accession>
    <name>A0A3B5ML76_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Desmosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plakin or cytolinker family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000025149"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025454.1">
        <property type="protein sequence ID" value="ENSXCOP00000025149.1"/>
        <property type="gene ID" value="ENSXCOG00000018784.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162855"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030057">
        <property type="term" value="C:desmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030056">
        <property type="term" value="C:hemidesmosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030506">
        <property type="term" value="F:ankyrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031581">
        <property type="term" value="P:hemidesmosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045104">
        <property type="term" value="P:intermediate filament cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042060">
        <property type="term" value="P:wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.780:FF:000001">
        <property type="entry name" value="Plectin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1290.10:FF:000001">
        <property type="entry name" value="Plectin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1290.10:FF:000002">
        <property type="entry name" value="Plectin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1290.10">
        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041615">
        <property type="entry name" value="Desmoplakin_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041573">
        <property type="entry name" value="Desmoplakin_Spectrin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043197">
        <property type="entry name" value="Plakin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035915">
        <property type="entry name" value="Plakin_repeat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001101">
        <property type="entry name" value="Plectin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169">
        <property type="entry name" value="ENVOPLAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23169:SF32">
        <property type="entry name" value="PLECTIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00681">
        <property type="entry name" value="Plectin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17902">
        <property type="entry name" value="SH3_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18373">
        <property type="entry name" value="Spectrin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21019">
        <property type="entry name" value="Spectrin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00250">
        <property type="entry name" value="PLEC"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75399">
        <property type="entry name" value="Plakin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Desmoplakin SH3" evidence="9">
        <location>
            <begin position="277"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Desmoplakin spectrin-like" evidence="10">
        <location>
            <begin position="373"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="484"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="587"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="740"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="903"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="979"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1513"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1752"/>
            <end position="1798"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="241"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="484"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="511"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="740"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="756"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="979"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1000"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1513"/>
            <end position="1533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1798" mass="204886" checksum="4D28BFCDCB4EE7D9" modified="2018-12-05" version="1">MTQIPMTQIPMTQIPVQVHSQVQQQSSRVRPELDEVTLRYVLDLLGWVEDNQKRVEEGEWGSDLPNQHNKHFLSASQRFHHHKPSSSLQSASKTRLRHLVELHAFVSAATKELMWLNEKEEEEINYDWSDKNSNMASKKESYSGLMRELELREKKVNGVQASGDKLLRDGHPARPTIEAFTAALQTQWSWLLQLCCCIETHLKENTAYFQFFADVKEAEERLRKMQEVMKRKYTCDRSVTVTRLEDLLQDAADEKEQLAEFQTHLEGLKRRAKTVVQLKPRNPASATKGKLPVQAVCDFKQMEVNECALLSNSQPFSWRVLNDRGSEAAVPSVCFLVPPTNKDAVNSVAGLDGNLQRLLAMWQTLFVDLKSLLSWQYLMRDVHIINSWNVTMVTTLRVDEYRLALRNLEQHYQDFLRDSPDSQMFGAEDRMQVESSYNRASQHYQTMLLKEEERRKLAEVQAELDKQKRLAEAQAQSAARAAQEAQELKLQMQQEASRRQGAAADSEQQKKNIQQELQQLRSLSEQEARSKSQQLQEALDSRSKIEEEIRLVRIQLETTVRQKTSAESELQSLRDRAAEADTLRRAAQDEAERLRRQVAEETQKKKTAEEELKRKAEAEREAAKQKQKALEELQRFKTQAEEAERRVKQAEEEQLRQVKVVEEVAQRSAATQMQTKASLFTEKTSQLEESLKKEQGAVLQLQEEAERLRKLQDEADRARQRAEQEVETWRQKANEALRLRLQAEEEAQKKSQAQQEAERQKVEAERDAKRRTKAEEAALKQKDGAEKELEKQRQFAEQIAQQKLSAEQECIRLKADFEHADQQRTLLDSELQRLKNEVSATESQRKQLEDELARVRREMDALLQMKVKAEQETLSKTEKSKQLLESEALKMKRLAEEAARLRAVAEEAQRQRQRAEEEAARQRAEADEILKEKLAAMDEATQVKTQAEIALKAKEAENERLKRKAEEEAYQRKLLEDQAAQHKQQIEEKMSQLKGSSQSELERQKTVVEETLRQKTKQYEDEVKKAKAFKEEQERQRALMEEERMKLQAVMDEAVKKQKEAEEEIKAKQKEVEELEKKRMEQEKLLEDENKKLREKLQSLEGTSKTGTTRTKEMEVQTDDVPEEQLVSMTMVGTTRKVLNGAVEVDRVKKDGGSPYAFDGIREKVPAERLFDLGVLTKKDLDKLRKGKVTVSRDLKKYLEGTSCIAGVYVESTKDRLSIYQAMKKNMIRPGTAFELLEAQAATGYVIDPIKNLKLNVTEAVKMAVVGPEFKDKLLSAERAVTGYRDPYSGQVVSLFQAMKKNLILKDHGIRLLEAQIATGGIIDPQESHRLPVETAYERGLFDEEMNTILTDPSDDTKGFFDPNTEENLTYLQLMERCMTDPETGLRLLLLKEKKRERKTSSKSSVRKRRVVIVDPESGKEMSVYEAYQKGLIDHQTYIELSEQECEWEEITITSSDGVVKSMIIDRRSGRQYDIDDAITRGLIDKSALDQYRAGTLSITEFADMLSGNMSGSRSRSSSFGSSSSYTASPVPSIKTPATTWNDPTEETGPVAGILDTDTLEKVSITEAIHRNLVDNITGQRLLEAQACTGGIIDPNTGEKFSVADAMNKGLVDKIMVDRISLAQKAHNGFEDPRTKSKMSAAQALKKGWLYYEAGQRFLEVQYLTGGLIEPDASGRVSIDEAVKKGTLDARTAQKLRDVSAYSKYLTCPKTKLKISYKDAMERSMIEEGTGLRLLEASSQSTKGLYSPYSISGSGSATGSRSGSRTGSRRGSFDATGSSFTTTFSSSSFGSPSYSRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MMA3</accession>
    <name>A0A3B5MMA3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-binding protein RFX6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Regulatory factor X 6</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MMA3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025549"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025857.1">
        <property type="protein sequence ID" value="ENSXCOP00000025549.1"/>
        <property type="gene ID" value="ENSXCOG00000019087.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244879"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031018">
        <property type="term" value="P:endocrine pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000211">
        <property type="entry name" value="Regulatory factor X, 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003150">
        <property type="entry name" value="DNA-bd_RFX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039779">
        <property type="entry name" value="RFX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057321">
        <property type="entry name" value="RFX1-4/6/8-like_BCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619:SF28">
        <property type="entry name" value="DNA-BINDING PROTEIN RFX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619">
        <property type="entry name" value="RFX TRANSCRIPTION FACTOR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25340">
        <property type="entry name" value="BCD_RFX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02257">
        <property type="entry name" value="RFX_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51526">
        <property type="entry name" value="RFX_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RFX-type winged-helix" evidence="11">
        <location>
            <begin position="84"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="696"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00077088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="872" mass="96281" checksum="BA22665DC7FB27BE" modified="2018-12-05" version="1">MKTFPLHAGKHFQKFLHVFQVNFGLNFPALLYFSAEDSNETPSSEDEHDLLELNSDLSMKQSNSSSRKTISQIIKDKKKQTQLTLQWLEENYIVCEGVCLPRCILYAHYLDFCRKEKLEPACAATFGKTIRQKFPLLTTRRLGTRGHSKYHYYGIGIKESSAYYHSVYSGKGLTRFSGSKLKNEGGFTRKYSLSSKTGTLLPDFPNAQHLVLQGNISREKVDTLIMMYKTHCQCILDNAININFEEIQNFLLHFWQGMPDHLLPLLENPIIVDVFCVCDSILYKVLTDVLIPATMQEMPESLLADIRNFAKHWEHWLASSLENLPECLAAKKLPIARRFVSSLKRQTSFLHLAQIARPALFDQTVVTDMVLDIDCVDLSSINSQPLLSASAAGDQDSDIYSEYDSVSVFQELKELLRKNATVESFIEWLDSVVEHKVIKPGKQCGRSVKKRAQDFLLRWSFFGARIMHNLTLNNASSFGSFHLIRMLLDEYILLALETQFNNDKEQDLQNLLEKYMKNADASKAAFMASPSSCFLANRSKAPTSVDQSVKNESLGGHAYMLLSTNQQQDLEAKAVLYQGNDSGQMDFSQVSGPLMTPPISPAALVHRGSVINQGPMAGRPLTPCSSYPETLYHCLPQSSGFFPAVSSGPSAQTYQTAPRFALYPALSKDGFYPPSVHHSSGSGDCSLTTYGAAVRPPSRYTPGPEPVQTEPGLDQQAGAPQILDAPDGFGFMGAGLNLAGGFYGNSSFLASQRVNPLVDQHVSVISSVGNLRPFSAAFSEVHDPLNILDEPGRKTSGSFFTNANILIERVVLSSGHHPLPPSGSATCMFGLPSPFSSQDALTSLHRGAASSEVQDLVSSLPPINTVFMGGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNS5</accession>
    <name>A0A3B5MNS5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNS5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021134"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021393.1">
        <property type="protein sequence ID" value="ENSXCOP00000021134.1"/>
        <property type="gene ID" value="ENSXCOG00000015809.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154405"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052458">
        <property type="entry name" value="PcG_PRC1-like_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46389:SF4">
        <property type="entry name" value="CHROMOBOX PROTEIN HOMOLOG 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46389">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN PC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="9"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="361" mass="39546" checksum="9C0EE636AB7FECD4" modified="2018-12-05" version="1">MTISVKKKIWFWSVILIKFSLFFSFCREREREMHGPKKRGPKPKNVVAKRCQKGAKPTSRTSSSRQNTPRSSSTASSRASSSSSAVPNALPFSSSSSSSAPAPSPKLNSLAATHKLKKDIHRCHRMSRRPLPRTDPTRSAFSSSSGFQSRMHVSPFSETVRILNRRVKPREVKRGRIILNLKVIDKPGRGGGGAAGSRNVQTGRQNIPSRNRIIGRKGEAPYRPFQPPLKMLGFPMYGKPFGLQCGGPMSFHSQTGSSSTTGARHSNSSSSQRRALPPSSSSSSTSTNVTAKHPQPATEASKESKSSKSPETQNSSTRFCVLYLFPHALPYSCAPTLLAWLFGQPLDSRDFSLMTVRISLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MR32</accession>
    <name>A0A3B5MR32_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein intermediate chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MR32"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025961"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026272.1">
        <property type="protein sequence ID" value="ENSXCOP00000025961.1"/>
        <property type="gene ID" value="ENSXCOG00000019390.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163996"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036157">
        <property type="term" value="C:outer dynein arm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000584">
        <property type="entry name" value="Dynein intermediate chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001723">
        <property type="entry name" value="Dynein intermediate chain 3, ciliary"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF7">
        <property type="entry name" value="DYNEIN AXONEMAL INTERMEDIATE CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="546"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="546"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="585"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="606"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="621" mass="71165" checksum="37591AB6F296A013" modified="2018-12-05" version="1">APSPLRTPGSSPPDVPGFPPRPRTAAAMEITYVYTKFRREFGRPGLFSDRAETVMVDIQPNPNLISNFVVKTPRDQATQACVDMCEHPVNTERFESNNSGVNHMEGAWPKDINPRDLEQTVRFKKKIEKEENYVNSLVHLGYVMDHCIRQNNTIDIYQEHFKEDTMEKVPELPTAKTMCVFRDPNKVKRTITGLSWHPDGGRKLAAAYCSLDFQKSPPDMSFDSYIWDLENPNYPEMALKPESPLVCLDYNPKDPHTLVGGSYSGQIAFWDTRRGSQPVEYSSLEHNHRDPVYKIIWLQSKTGTDAFSASTDGQILWWDVRKLSEPTERLVLDPSREGNLDRALGAISLEFEPTMPTKFMVGTEQGMVVSCNRKAKTPAEKIVCTYEGHHGPIYAIQRNPFFPKNFLTVGDWTARIWSEDIKESSIMWTKYQMSYLMDACWSPVRPSVFFTVKMEGVLDIWDLLFKQREPTLSVKVSDKPLYSLRIQDNGQVLACGSHSGEAALLQISSGLSTLQKNEKSLMAAMFERETKREKILEARQREIRLKERSRSEQSREDEAGREDGDDDPEELLAKVESDFYSLVETEMRKREKERRAAAPQRQPRPRQSESSEKKEEETLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRY2</accession>
    <name>A0A3B5MRY2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphorylase b kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PHKB</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Glycan biosynthesis; glycogen metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Lipid-anchor</topology>
            <orientation evidence="1 8">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="7">Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the phosphorylase b kinase regulatory chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRY2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023941"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024226.1">
        <property type="protein sequence ID" value="ENSXCOP00000023941.1"/>
        <property type="gene ID" value="ENSXCOG00000017866.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183118"/>
    <dbReference type="UniPathway" id="UPA00163"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005964">
        <property type="term" value="C:phosphorylase kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045583">
        <property type="entry name" value="KPBA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008734">
        <property type="entry name" value="PHK_A/B_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749:SF8">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19292">
        <property type="entry name" value="KPBB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="6 8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 8" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="7 8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="GH15-like" evidence="11">
        <location>
            <begin position="48"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="GH15-like" evidence="11">
        <location>
            <begin position="170"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphorylase b kinase regulatory subunit alpha/beta C-terminal" evidence="12">
        <location>
            <begin position="841"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="618"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="623"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="S-farnesyl cysteine" evidence="7">
        <location>
            <position position="1010"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608734-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF19292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1018" mass="116581" checksum="0179C7B930608FBB" modified="2025-10-08" version="2">MRVNMASLADVGWKLLEFKARSKRSGSIYEPLKLSNLQREDEPLWEKLDRYYNAVKTTILNYQSPTTGLFPVKTFSDMKEAKVRDSLYCAACAWALGMAYRRIDDDLGRTHELEHSAIKCMRGILYCYMRQADKVSLFQRLLLSCCFPQESLLLFQLITFIVVIMWLIFGVSSHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLSSLLPRESRSHNTDAALLPTISYPAFAVDDDALYSQTLDKIVRKLRGKYGFKRFLRDGYRTANEDKNRRYYKPAEMKLFDGIECEFPMFFIYMMIDGVFRGNKAQVKEYQDLLEPIIFQSFEGHAVIPKYYYVPADFVEAEQNKHGSQKRFPSNSGRDGKMFLWGQAMYNIAKLLVDELINPKDIDPVHRYVPRQDQRNVSMRYSNQVNSFLLQVFLNTYGIQTQTPQQVEPIQIWPQKELVKAYRFLAVNKKLGLSGRPDRPVGCIGTCKIYRILGKTVVCYPIVFDLSDFYLSQDVMLLIDDIKNALQFIKQCWKMQGRPLFLVLIREDNIKGSRFNPVLDMLASFKKGVIGGVKVHVDRLQTLISGAFVEQLDFLRVNEAEIPEFKSFEELELPKHSKVKRQTSTPNASDLEQQPEISVGEWQNKPTYEILQKFHDCNCLASQAQLACILLRREGPDFLAKDGNLMDELERIYRRAGSRKLWLAVRHAAAITRKYASSIAPHITTILVHGKQVTLGLFGHEEEVISNPLSPGVIQGIIYSKCSPQGGEREAVLQQELVIHIGWIISNNPELFSGMLKIRVGWIVQAMKHELKIRGGDLAPQDIYQLSPSNIKQLLLDVLQPQHTARSWLNRRQIDGSLNRTPLGFYDRVWQILERTPNGIVVGGTHLPQQPTLSDMTMYEMNFSLLVEDTLKNIVLPEYRQIIVELLMVVATVLERNPELEFGDKVDLDSLVKEAFNDFQRDRSRFEGMEKQDDMEVFYNTPPLGRRGTSSYLTKAVMIQLLQGDMKPCKDDPCAVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MT45</accession>
    <name>A0A3B5MT45_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">histone acetyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">KAT2B</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21992"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:21993"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. GCN5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MT45"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026938"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027269.1">
        <property type="protein sequence ID" value="ENSXCOP00000026938.1"/>
        <property type="gene ID" value="ENSXCOG00000020122.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154995"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140672">
        <property type="term" value="C:ATAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043992">
        <property type="term" value="F:histone H3K9 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060349">
        <property type="term" value="P:bone morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060173">
        <property type="term" value="P:limb development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903010">
        <property type="term" value="P:regulation of bone development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061035">
        <property type="term" value="P:regulation of cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05509">
        <property type="entry name" value="Bromo_gcn5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000004">
        <property type="entry name" value="Histone acetyltransferase KAT2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000014">
        <property type="entry name" value="Histone acetyltransferase KAT2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037800">
        <property type="entry name" value="GCN5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750">
        <property type="entry name" value="GH11602P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750:SF2">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 14" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="16">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="15">
        <location>
            <begin position="207"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="299" mass="35086" checksum="0C6D5CF122F8C2A8" modified="2018-12-05" version="1">MPKEYITRLVFDPKHKTLSLIKDGRVIGGICFRMFPSQGFTEIVFCAVTSNEQVKGYGTHLMNHLKEYHIKHKILNFLTYADEYAIGYFKKQGFSKDIKVPKAKYVGYIKDYEGATLMGCELNPSIPYTEFSVIIKKQKEIIKKLIERKQAQIRKVYPGLSCFKDGVRQIPIESIPGIRTLLRTARKELKDPDQLYSTLKTILQHVKNHQNAWPFMEPVKKTEAPGYYQVIRFPMDLKTMSERLKSRYYTTRKLFMADMQRIFTNCREYNAPESEYYKCANILEKFFYTKIKEAGLIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MTR5</accession>
    <name>A0A3B5MTR5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyrosyl-DNA phosphodiesterase 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">5'-tyrosyl-DNA phosphodiesterase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">PML body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027891250.1">
        <property type="nucleotide sequence ID" value="XM_028035449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MTR5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026891"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027220.1">
        <property type="protein sequence ID" value="ENSXCOP00000026891.1"/>
        <property type="gene ID" value="ENSXCOG00000020082.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114155526"/>
    <dbReference type="KEGG" id="xco:114155526"/>
    <dbReference type="CTD" id="553516"/>
    <dbReference type="GeneTree" id="ENSGT00390000014242"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070260">
        <property type="term" value="F:5'-tyrosyl-DNA phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09080">
        <property type="entry name" value="TDP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14344">
        <property type="entry name" value="UBA_TYDP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000024">
        <property type="entry name" value="Tyrosyl-DNA phosphodiesterase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051547">
        <property type="entry name" value="TDP2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15822">
        <property type="entry name" value="TRAF AND TNF RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15822:SF4">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="14">
        <location>
            <begin position="114"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="74"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="359" mass="40572" checksum="D710E5629C77340C" modified="2018-12-05" version="1">MASTSNSESPSVSDLEKSRSGLCEEFATITGTDSAVAQCYLAENDWEMERAVNSFFEADMERIFEEDFQESDCVPPKKKQKVEEEPSKECIDLTGDSPVARKPPDEDDGKLSLISWNVDGLDTDNLAERARGLCSYLVLYTPDVVFLQELIPPYVQYLKKRAVSYLIIEGGEEAYFTGMMLKKSRVKFLESEIVTYPTTQMMRNLLVAQVTFKGQKLCLMTSHLESCKGHAQERMKQLHTVMQRMKDAPDDVTVLFGGDTNLRDTEVAKVGLPSGVCDVWERLGKQEHCRYTWDTKANNNKAVPFVSRCRFDRIYLRPASKEGVPRLAPDHMALIGLEKLDCGRYTSDHWGLYCSFTAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUN4</accession>
    <name>A0A3B5MUN4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 6</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MUN4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023199"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023477.1">
        <property type="protein sequence ID" value="ENSXCOP00000023199.1"/>
        <property type="gene ID" value="ENSXCOG00000017334.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234535"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003828">
        <property type="term" value="F:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000001">
        <property type="entry name" value="ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050943">
        <property type="entry name" value="Glycosyltr_29_Sialyltrsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012163">
        <property type="entry name" value="Sialyl_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987:SF50">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE 8F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005557">
        <property type="entry name" value="Sialyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017199109" evidence="13">
        <location>
            <begin position="26"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="108"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR005557-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="323" mass="36776" checksum="DADE737798919F51" modified="2018-12-05" version="1">MRGQILKPLLSLMITILLLVIPLRHETEPHFGYILCCTESSDYLWVPRSQLSAKCHGLEKATITQANTPVGTKLVYDGEKKRTLQVNPEIFSTFFKGHPFPNKTWHTCVVVGNGGILANSSCGKMIDSAEFVIRCNLPPLSNEYEKHVGINTDLVTANPSILMEKFGALMGRRRKFVESLRSYGDSLLLLPAFSFGHNTPVSLRAFYTLEDFKSPVRPIFFNPGYLHNLALFWRSQGLRTIRLSTGIIMTSLALELCDNVQLYGFWPFDFHPYSFQTLTNHYYDDRKTKTKFHAMPDEFNHLLRLHSQGVLKLHLGHCKAEPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUZ7</accession>
    <name>A0A3B5MUZ7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SPRY domain-containing protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SPRYD7</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MUZ7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025097"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025402.1">
        <property type="protein sequence ID" value="ENSXCOP00000025097.1"/>
        <property type="gene ID" value="ENSXCOG00000018738.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011048"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd12880">
        <property type="entry name" value="SPRYD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035766">
        <property type="entry name" value="SPRYD7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20951">
        <property type="entry name" value="C13ORF1 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20951:SF2">
        <property type="entry name" value="SPRY DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="2">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00021772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="188" mass="21045" checksum="AFF72B4304B75BD8" modified="2018-12-05" version="1">LEQSHQCCFQFISSFVRCKHPSKTQYGSTDVVIVKSGRRICGTGGCLANAPLHQNKSYFEFKIQSSGVWGIGVATQKVNLNQVPLGRDTNSLVLRHDGSVYHNDEEKNRLPANSLPQEGDIVGITYDHVEMNLYLNGKNMHCPASGIRGTVYPVVYVDDSAILDCQFSDFYHTPPQGFEKILFEQQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVD4</accession>
    <name>A0A3B5MVD4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Hexosyltransferase</fullName>
            <ecNumber evidence="15">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Golgi apparatus membrane</location>
            <topology evidence="2 15">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVD4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027521"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027855.1">
        <property type="protein sequence ID" value="ENSXCOP00000027521.1"/>
        <property type="gene ID" value="ENSXCOG00000020545.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156219"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008499">
        <property type="term" value="F:N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000001">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF20">
        <property type="entry name" value="BETA-1,3-GALACTOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 15" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="328" mass="38108" checksum="CAB9E3C8509D30A2" modified="2018-12-05" version="1">MPSKVSCLYLLTVVCWASALWYLSGSRPSSSYVGQVSIPMRKAPKVSKNSTFSNIRTRSLNPHDFGYLINEAKKCEAEPPFLVILISTTHKEFNARQAIRETWGDETTFPDVRVVTLFLLGRSIDTVLNQMVEQESQIFHDIVVEDFIDSYHNLTLKTLMGMRWVATFCPKSQYVLKTDSDIFVNMENLIYNLLKPTTKPRRRYFTGYVINGGPIRDMRSKWYMPRDLYPESKYPPFCSGTGYVFSADIAELIYKTSLHTRLLHLEDVYVGVCLRKLGIHPFQNSGFNHWKMAYSLCRYRRVVTVHQISPEEMHRIWNDMTSKKHLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXV6</accession>
    <name>A0A3B5MXV6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcriptional repressor scratch 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Scratch homolog 1 zinc finger protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the snail C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXV6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026926.1">
        <property type="protein sequence ID" value="ENSXCOP00000026602.1"/>
        <property type="gene ID" value="ENSXCOG00000019861.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154491"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000560">
        <property type="entry name" value="Scratch family transcriptional repressor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000043">
        <property type="entry name" value="Scratch family zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000169">
        <property type="entry name" value="transcriptional repressor scratch 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000207">
        <property type="entry name" value="zinc finger protein SNAI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF60">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR SCRATCH 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="177"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="59"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="269" mass="30030" checksum="276592018620BDFE" modified="2018-12-05" version="1">MPRSFLVKKVKLDDFSAAALETSYSRREELRLRFHNHDKGFHSSIIYGHINNTYSGIHSDIHGDSDQPDSPRSEVSSSAGGYINGDAAVSEGYSVDAFFIMDGRSRRKAAANPRGATQRHTCSECGKTYATSSNLSRHKQTHRSIDSKMAKKCPTCGKVYVSMPAMAMHLLTHDLKHKCDVCGKAFSRPWLLQGHMRSHTGEKPFGCAHCGKAFADRSNLRAHMQTHSAFKHYKCKRCDKTFALKSYLNKHYESACFKGAFSPLSSLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MYM1</accession>
    <name>A0A3B5MYM1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Chromatin assembly factor 1, subunit B</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat HIR1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MYM1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025450"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025757.1">
        <property type="protein sequence ID" value="ENSXCOP00000025450.1"/>
        <property type="gene ID" value="ENSXCOG00000019012.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074968"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033186">
        <property type="term" value="C:CAF-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048568">
        <property type="term" value="P:embryonic organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060041">
        <property type="term" value="P:retina development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000852">
        <property type="entry name" value="Chromatin assembly factor 1 subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055410">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045145">
        <property type="entry name" value="PTHR15271"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15271">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15271:SF4">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24105">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CAF1B/HIR1 beta-propeller" evidence="11">
        <location>
            <begin position="1"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="62"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="126"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="168"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="391"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="399"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="423"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="444"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="469"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="510"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="537" mass="58613" checksum="10D9D6D63EC149EB" modified="2018-12-05" version="1">MKVVTCEIAWHNKEPVYSLDFQHNTEGRFHRLATAGVDTTVRLWRVEQGPDGKAVVEFLSNLARHSKAVNVVRFSPNGELLASGGDDAAILLWKLNDSKEPEQATVFQDEEDAQLNKESWSVVKTLRGHLEDVYDICWTRDGNFMISGSVDNTAIMWDISKGQKLCMLNDHKSYVQGVTWDPQGQYVATLSCDRVMRVYSTHTKKKAFCISKMSSGPLADGEVKQYRMFHDDSMRSFFRRLSFTPDGSFLLAPAGCVEVGENIINTTYIFSRKSLKRPIAHLPCPSKATLAVRCCPALPNLFQLPYRMVFAVASEDSIFLYDTQQSLPFGLVSNIHYHTLSDLTWSSDGSFLAVSSTDGYCSFLSFSPGELGTPLKEPPVLEIFVPNSGVEKKGKKSSASKNSSPVSQPSSSTPSSASQTSSGKKDSPCPTPTDEKKTTPKPRSTPQPRRITLNTLEGWGKPTTPKPSTPSASQTPSSGSTSAPSTPQPSVTPLTPTSSSRNQPRHAPLTPSTSKVLNTAGSTTPKGPTTPKGPTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N031</accession>
    <name>A0A3B5N031_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Testis expressed 10</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the IPI1/TEX10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N031"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026325"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026644.1">
        <property type="protein sequence ID" value="ENSXCOP00000026325.1"/>
        <property type="gene ID" value="ENSXCOG00000019657.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182992"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071339">
        <property type="term" value="C:MLL1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024679">
        <property type="entry name" value="Ipi1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057949">
        <property type="entry name" value="TPR_TEX10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16056">
        <property type="entry name" value="REGULATOR OF MICROTUBULE DYNAMICS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16056:SF2">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12333">
        <property type="entry name" value="Ipi1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25781">
        <property type="entry name" value="TPR_TEX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="HEAT" evidence="5">
        <location>
            <begin position="102"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-rRNA-processing protein Ipi1 N-terminal" evidence="7">
        <location>
            <begin position="135"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="TEX10-like TPR repeats" evidence="8">
        <location>
            <begin position="559"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="932" mass="104328" checksum="6F1BC60ABA60C1DB" modified="2018-12-05" version="1">MKAKKKKRQDDFQKVKLKVGKKKPRADNATNTNFRTRGICLPDQLKRDTSGPTTHRQLGINVSSAELQNKPNIYIEFQNALLGLKELLSLSPSLLEQHLSRLLSEVAAVLSDKDGNVRVAATRSLRFIAQSVPAEQVAPFFPLLSAHLTCAMTHIEPGIQEDAMNVLDIFLEHYPSLLASRPVVLLTNFLELISHRQNSGGARKTQDARGRTWTLSVHPDRRVTSQQWRLSVLLRLGNFLQAIVEERPTEEGDLSMRTEGVFGSSGMSRLTPLNLNWEELTYKKIDVKVYENSGATPTPHSTFKLRPEMDPGGVGSKGLDSAETVQSFAGTLVPLLLEVWVEACAGDCSWNSTEGAHLLTPDTTSLMFQVLSILQLLRKLPTHRENKEALDAWFRKEYLEDFKQHFMKNFPYGLRDTPKHKKKVDLKRSKQTAAIPSSTVEPLALNITLCQVMVSLSQKQGLGHEADGDWLSPLRAFVRDTLGNGVKLSYRQLHMLLRTVWKMVLTQQSRAVTEELLAAVYIYYKQKNLPLQTRSLLLSFYSKLYLQEQEHTHIARSKVLCRWLASLPVQLSQLGHRNPALSAQLIVSIHAAASRGNKELLNSLQTYACRLYDPQDGVVVLLPAESQQRMVQLLYFLPKMPQPLLANLSGCCTAGRISATLAASLIRILHFRSSLSGWSVGSQEAALQDVDYISFLFSTLAGFSSEHLAALQDDETALLPSSLSPLCLYQTPLEQFTHHWDVVEEVCHSLETIGSKSQCFDILQNGIFRYLVGLEVVPDSMSAGLLRAVSRLLDLSILPAEQVLRFLSQCCLSLLVLLFVFREAIWSSCVTALSSVPRLLRIVLQSMHVGDLSAEELPQLGQILSMLLQHTPLHNQLMANAVLLQELIQNLTRFFRGATREQWLTDLLYCYSVTVAHGSSAHRGSMGLRDMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N139</accession>
    <name>A0A3B5N139_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carbohydrate sulfotransferase</fullName>
            <ecNumber evidence="11">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sulfotransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5N139"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027155"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027488.1">
        <property type="protein sequence ID" value="ENSXCOP00000027155.1"/>
        <property type="gene ID" value="ENSXCOG00000020271.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157128"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030166">
        <property type="term" value="P:proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018011">
        <property type="entry name" value="Carb_sulfotrans_8-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137:SF2">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="9 11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="361" mass="42079" checksum="0D33ABE68AB6C689" modified="2018-12-05" version="1">MRHQWLLLGACGWVLLVLMFVSKFISFKTIEGKRPVIDTISWWEKNHKTLDGKLSGDIAEAYRSQRIVTVIKPEDYWQAISESRIEHLSAVCKNSSLSNLTHVSISKFVLDRIFVCDKRQILFCQTPKVGNTQWKKVLIVLNGGFPSVEEIPENLVHDHDKNGLPRLSSFSPQEITQRLNSYFKFFIVRDPFERLISAFKDKFVKNPRFEPWYKHDIAPAIIRKYRRNHRESDLAASGLHFEDFVRYLGDAEGRRRMDRQFGEHIIHWVTYAELCAPCEINYSVVGHHETLEHDAAFILRAAGIHQLVSYPAIPPGITRYNKTKVEHYFSGISKRDIRRLYARYHGDFQLFGYPTPAFLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KJ84</accession>
    <name>A0A3B5KJ84_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KJ84"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000158"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000163.1">
        <property type="protein sequence ID" value="ENSXCOP00000000158.1"/>
        <property type="gene ID" value="ENSXCOG00000000156.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000176898"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902936">
        <property type="term" value="F:phosphatidylinositol bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028068">
        <property type="entry name" value="PIRT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16100:SF4">
        <property type="entry name" value="PHOSPHOINOSITIDE-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16100">
        <property type="entry name" value="PHOSPHOINOSITIDE-INTERACTING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15099">
        <property type="entry name" value="PIRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="154" mass="16783" checksum="C496F8CDAC154C1A" modified="2018-12-05" version="1">MPGSPENIPLGECALSQSQDRLTPPSHAASTVFSLSRSESLWTPEPPPKRCDVFWFPIHFMSTGGSFLACGVIISGLCFAGQCQPAPALLGPALLSIGLMVLVVGVVLIPITKKNRKRSHMRSLLNMKLSGCIFSFKLNAYFIFSHKLNIYFAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KJC7</accession>
    <name>A0A3B5KJC7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000000213"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000219.1">
        <property type="protein sequence ID" value="ENSXCOP00000000213.1"/>
        <property type="gene ID" value="ENSXCOG00000000213.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154065"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052573">
        <property type="entry name" value="DnaJ_C_subfamily_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018961">
        <property type="entry name" value="DnaJ_homolog_subfam-C_membr-28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39158:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39158">
        <property type="entry name" value="OS08G0560600 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09350">
        <property type="entry name" value="DJC28_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="20"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="359" mass="40300" checksum="2E2739108BFBFDCB" modified="2018-12-05" version="1">SSRRSRWFSSDDRISRSLRESYRVLQLPAEGRSSPAEVRAAYLRLAKLYHPDCGEPTADAGLFALVEEAYRAVLAHQSKTQQPDGATEDDEDKSRGNALQHRHYLSYEGVGSGTPSQRERQYRQVRVDRAAEQVLSYRQREHERAAAADGAMAERDARQRSHKIKITQAVERLVEDLIQESMARGDFRNLSGAGKPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KKA2</accession>
    <name>A0A3B5KKA2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">[histone H3]-trimethyl-L-lysine(9) demethylase</fullName>
            <ecNumber evidence="4">1.14.11.66</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(9)-[histone H3] + 2 2-oxoglutarate + 2 O2 = N(6)-methyl-L-lysyl(9)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:60200"/>
            <dbReference type="Rhea" id="RHEA-COMP:15538"/>
            <dbReference type="Rhea" id="RHEA-COMP:15542"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JHDM3 histone demethylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.66" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KKA2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000613"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000623.1">
        <property type="protein sequence ID" value="ENSXCOP00000000613.1"/>
        <property type="gene ID" value="ENSXCOG00000000526.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154930"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051864">
        <property type="term" value="F:histone H3K36 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140684">
        <property type="term" value="F:histone H3K9me2/H3K9me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20463">
        <property type="entry name" value="Tudor_JMJD2A_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20466">
        <property type="entry name" value="Tudor_JMJD2A_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000048">
        <property type="entry name" value="Lysine-specific demethylase 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000029">
        <property type="entry name" value="lysine-specific demethylase 4C isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.70:FF:000001">
        <property type="entry name" value="Putative lysine-specific demethylase 4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040477">
        <property type="entry name" value="KDM4-like_Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047479">
        <property type="entry name" value="Tudor_KDM4A_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047481">
        <property type="entry name" value="Tudor_KDM4A_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF51">
        <property type="entry name" value="[HISTONE H3]-TRIMETHYL-L-LYSINE(9) DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18104">
        <property type="entry name" value="Tudor_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="18">
        <location>
            <begin position="14"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="19">
        <location>
            <begin position="142"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="20">
        <location>
            <begin position="680"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="429"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="973" mass="110453" checksum="84F1A656BAF00D56" modified="2018-12-05" version="1">MTTDTPDQTPNFRVMTFTPSKEEFKDFVRYVAFMESQGAHIAGIAKIIPPKGWKPRHTYDDIDDLLIPAPIQQVVTGQSGLFTQYNIQKKPMTVVEFQKTSNLAKFCSPRYVDFDELERKFWKNLTFNPPLYGADVSGTLFDPDVSEWNMAHLNTILDTVESESAVKIKGLTTPCLYFGMWKSAFPWHTEDMNLYSICYLHYGEPKSWYVVPPEHGRRLERLAKGFFPGNAQSCEAFLRHKMTLISPSILKKYGIPYEKVTQEAGQFIVTFPFAYHAGFNHGFNCAESTNFATQRWIDYGKQAKLCSCRQDMVKISMDAFVRKFQPDRYKLWKAGKDDTPIDHVKPTPEAAEFLKKEKASPCKKVSSEASSEEPTSPAQKDKRVVIKCFIFNTAQPKSERQLEKHIKPPPKCISKANKKTSNRLISARKDKSGGRAADMSPLLPVEGNAEGSVEPRVETSNIPVHKLFQRTLSPADVLHVHSYAKGDYGEEILVQEEEQAIRSDKKEDIKHVSSVGICDSTSIQIIQLPPVEAGTFEGDNWAKPLTHLWQSSPPNLKKEREYNQRMGSKPPYCSICMLFQTYQQVFLSIRENTTPERFSDSDMKMQTKPLIPEMCFTITTEEDAESEEQPVAHYLEEDGTSLLISCSQCSVWVHASCYGVNPASVSTEWKCARCKANAMNESCCLCLLRGGALHKTTNDKWVHVLCAVAVMEARFVNIAERSPVDLSGIPLQRFKLKCYYCKKRVKKASGCCVQCSHGRCPTAYHPTCAQAAGVLMQPDDWPFVVHVTCCRHKGPAQIERNKAAMHELTVGQKVICKHKNGRYYQCDVVQLSKETFYEVNFDDGSFSDNLFPEDIVNQDCAQLGPPPQGEVVQVRWTDGLVYGAKFVAAHVIQMYLVEFEDGSQLTAKRDDVYTLDEELPKRVKSRLSKASDMRFDGIFEDNEIIQESKRQRVINSRYRGDYIEPVIYRAIME</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQT3</accession>
    <name>A0A3B5KQT3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">E3 ubiquitin-protein ligase TRIM71</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Protein lin-41 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">RING-type E3 ubiquitin transferase TRIM71</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Tripartite motif-containing protein 71</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance. Binds to miRNAs and participates in post-transcriptional repression of transcripts. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 29">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_027890924.1">
        <property type="nucleotide sequence ID" value="XM_028035123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQT3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002483"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002517.1">
        <property type="protein sequence ID" value="ENSXCOP00000002483.1"/>
        <property type="gene ID" value="ENSXCOG00000001981.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114155302"/>
    <dbReference type="KEGG" id="xco:114155302"/>
    <dbReference type="CTD" id="131405"/>
    <dbReference type="GeneTree" id="ENSGT00940000159099"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035198">
        <property type="term" value="F:miRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19812">
        <property type="entry name" value="Bbox1_TRIM71_C-VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19796">
        <property type="entry name" value="Bbox2_TRIM71_C-VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14954">
        <property type="entry name" value="NHL_TRIM71_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16589">
        <property type="entry name" value="RING-HC_TRIM71_C-VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000013">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000025">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000080">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000923">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000435">
        <property type="entry name" value="Tripartite motif containing 71, E3 ubiquitin protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050952">
        <property type="entry name" value="TRIM-NHL_E3_ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NHLRC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24104:SF55">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51125">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="16" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="25">
        <location>
            <begin position="12"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="26">
        <location>
            <begin position="152"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="26">
        <location>
            <begin position="235"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="23">
        <location>
            <begin position="441"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="24">
        <location>
            <begin position="556"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="24">
        <location>
            <begin position="603"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="24">
        <location>
            <begin position="650"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="24">
        <location>
            <begin position="697"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="24">
        <location>
            <begin position="744"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="24">
        <location>
            <begin position="791"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00040205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00043228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00059249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="831" mass="91430" checksum="1021BDB158A7C2EC" modified="2018-12-05" version="1">MASFPDSDLQTCPLCKELCGSSAPISSSSSTSSSSSHTSGSSNHATKRLHVLPCLHTFCRQCLEGQRSPSDPLKLRCPTCDHKVSISEAGVDSLPSSNFLFSNLLDVVVSSEDQIQNKNGHHHLGGLIGGSSGFHPSHGGLLHAQHMGEPQCSSCDEENPATSHCLDCQEYLCDNCVRAHQRVRLTKDHFIERLGESLHLSRVNSNSSGTGQPGVSVSLAQSLQNNFALLSLFQDRMSFCQHHDSEVFLFFCETCSVPICRECSVGRHMGHTFVYLQDAVQDCRAITIQLLADAQQGRQAVQLSMEKVQAMAEQVEIKAKVVQTEVKALVLRHRKALEERECELLWKVEKIRQVKAKSLYLQVEKLHQSLTKLDSTIAAVSQILDEGHHLDVLLARERMLTQIHELKTLRGLLQPQEDDRFMFTPPDQALYIAIQSMGLISSSAFAPVTKAHGEGLKNVLRGKPASFTVIGYNHDGEPRLSGSDPVSVMVMSVADGSPSAVEVTDHQNGSYTVSYLPKTEGEHLVSVLVCNQHIQGSPFKVIVKSGRSYGSLGSQVSSFGCEGEGDGQLCRPWGISVDKEGYVVVADRSNNRIQIFKPCGAFHHKFGSLGSRPGQFDRPAGVACDSQRRIIVADKDNHRVQVFTFEGQFLLKFGEKGTKNGQFNYPWDVAVNSEGKILVSDTRNHRVQLFAPDGSFLNKYGFEGALWKHFDSPRGVAFNHEDHLVVTDFNNHRLLVIRPDCQSARFLGSEGTGNGQFLRPQGVAVDQENRIIVADSRNHRIQVFEPNGNFLCKFGTQGSGYGQMDRPSGVAVMPDGVIVVVDFGNNRILKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRI6</accession>
    <name>A0A3B5KRI6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATED"/>
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        <location>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001097.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="137" mass="15298" checksum="BF68A8B6F0124BF3" modified="2018-12-05" version="1">MRCSPGANILRDSVGNVKLGDFGASRRLQTICLSGTGIKSVTGTPYWMSPEVISGEGYGRKADIWSVGCTVVEMLTQRPPWAEFEAMAAIFKIATQPTNPVLPPHVSDHCREFLRRIFVETKHRPSADELLRHFFLH</sequence>
</entry>
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    <accession>A0A3B5KSU6</accession>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <authorList>
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            <end position="38"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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    <accession>A0A3B5KSZ5</accession>
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        <recommendedName>
            <fullName evidence="1">BUB1 N-terminal domain-containing protein</fullName>
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    </protein>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <begin position="28"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000731.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="120" mass="13702" checksum="7D66EE247036203E" modified="2018-12-05" version="1">MKVRFCCNPWCWKLLHCSGVHGNRVFQASYCVDPANLYAHVFSRGVGSRTAALYLAWARHFEQKGMKDQADAVYLKHHVGRPPQLAGRLSQQHHRLRPAGPVRLHTVHSQNAIQWRLLPG</sequence>
</entry>
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    <accession>A0A3B5KSZ8</accession>
    <name>A0A3B5KSZ8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
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            <authorList>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
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        <property type="entry name" value="MYOSIN PHOSPHATASE RHO-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17271">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY PH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
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        <location>
            <begin position="80"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="190"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="350"/>
            <end position="392"/>
        </location>
    </feature>
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        <location>
            <begin position="492"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="555"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="714"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="203"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="381"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="835" mass="93776" checksum="FE65C143F6C160EF" modified="2018-12-05" version="1">SFSRLLHQILPNVDPHVTLQPSTLPQGTINMNQCLDVVDGEARTGQKSSLCILTPDKEHFIRAECREIINGWQEALTVYPRTNRQNQKKKRKVDPPTHQEPGPAKVTVTSGSMADAEQIPTSRTTLWQEQSRWNRAAIPCSRSASCLSQLGQSQPDDRMFDLTCSCVSDGGAVSSGRKVRVESGYFSLEKTKSEPNPAPPQHLPLSSSASSSSLGASRYEPDLPTSQRSPDSLPSPKALVSPSYSTISSSQSSLDSEPSSQALPTWAGGTTRGASELDGEIDLSTCYDVKEFPVQRNYGFQILCKDGACTLSAMTSGIRRNWIQAIMKNVRPTVAPDVTRHVMLEPCQVTSDPKLDDSKQSPGSNAATPPSEPRRKGVRERRREGRSKTFDWTEFKMERKEKPVKERAETIDLNEPSPEERGKMDVDHRAVGLWGADATDGVTPNIRQEIEQRWHQVETTPLREEKQVPISTTAGTAERLPANERLPANELAALLDKELGQKQKELDRLQEQNSLLKEQLEDALGREQSAREGYILQATCERGFAAMEETHQKVVEDLQRQHQREISKLMEERERLLAEETAATIAAIEAMKNAHKEELEKNQRSHLSGLNSDIDELRLQYEEELQSIHRELEVLSEQYSQKCLENAHLAQALEAERQALRQCQRENQELNSHNQELNNRLTAEITRMRSCFSGETALSPLTHGKDVYELEVLLRIKESEIQYLKQEIHSLKDELQSALRDKKYATDKYKDIYTELSIVKAKADCDISKLKEKLLMATEALGERNVDDAVTSGYDIMKSKSNPDFMKKEKSTPPKQLRGLRSKVSDVVVVVLFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KUY7</accession>
    <name>A0A3B5KUY7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate dehydrogenase assembly factor 3</fullName>
            <shortName evidence="6">SDH assembly factor 3</shortName>
            <shortName evidence="6">SDHAF3</shortName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Promotes maturation of the iron-sulfur protein subunit of the SDH catalytic dimer, protecting it from the deleterious effects of oxidants. May act together with SDHAF1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with the iron-sulfur protein subunit within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the complex I LYR family. SDHAF3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KUY7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003933"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003977.1">
        <property type="protein sequence ID" value="ENSXCOP00000003933.1"/>
        <property type="gene ID" value="ENSXCOG00000003095.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010029"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006105">
        <property type="term" value="P:succinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20270">
        <property type="entry name" value="Complex1_LYR_SDHAF3_LYRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008381">
        <property type="entry name" value="SDHAF3/Sdh7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137">
        <property type="entry name" value="DC11 ACN9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137:SF6">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="70" mass="8150" checksum="7D8C22C6CEE14648" modified="2018-12-05" version="1">MAASAHVSTIRSLYKRILVLHRFLPIHLRALGDQYVKEEFRRHKSAAPEEVKSFLLEWEVSDSSTEQRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KWS9</accession>
    <name>A0A3B5KWS9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KWS9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000029"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000029.1">
        <property type="protein sequence ID" value="ENSXCOP00000000029.1"/>
        <property type="gene ID" value="ENSXCOG00000000026.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000271609"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.30:FF:000022">
        <property type="entry name" value="Transmembrane agrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017254913" description="Kazal-like domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="2">
        <location>
            <begin position="25"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="79" mass="9158" checksum="760F20B88190DD9F" modified="2018-12-05" version="1">FFFFFTFVVLFLCKDCLFNAVCVVEQSGARCSCDPIECDDTYKPLCSVDGRTFPNNCWRRKAECLSKSLIRTKILRKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KY59</accession>
    <name>A0A3B5KY59_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Otolin 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KY59"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002571"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002606.1">
        <property type="protein sequence ID" value="ENSXCOP00000002571.1"/>
        <property type="gene ID" value="ENSXCOG00000002059.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155435"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045299">
        <property type="term" value="P:otolith mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051259">
        <property type="term" value="P:protein complex oligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000001">
        <property type="entry name" value="Complement C1q B chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427:SF51">
        <property type="entry name" value="OTOLIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017313713" evidence="8">
        <location>
            <begin position="24"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="9">
        <location>
            <begin position="343"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="25"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="132"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="207"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="262"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="308"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="474" mass="49502" checksum="953E1EE0F5B4FE4D" modified="2018-12-05" version="1">MPSADLILVTTVFMAFIITLTSGGRTTRWPKPQNTNKPPQTGTSGFGGGLRSGQIATKNPSPSSSLHTEETTGFMTDGYSLSPTDSSIFNSEAYPTEYLTDAILPPGNAPGNYTFDYNECFFNVCECCPPEKGPEGPTGEKGSPGERGPPGLPGEKGEIGPPGSPGPAGLPGLSGLNGEKGSTRMKRKILVDALFLLSACDLGFKGEKGENGINGLKGDAGEKGEPGHNGTMGTIGPMGPPGTAGTKGQKGEQGRLGECLLGERGQKGDLGDPGPPGPKGEMGPQGANGSDGSKGEMGPAGEKGETGARGPPGARGVAGMKGERGVKGVRGPRGPKGDPGESLEPVRSAFSVGLFPSRSFPPPGLPVKFDKVYYNEEGHWDPALNKFNVTHSGVYLLTYHITVRNRPLRVALVVNGVRKVRTRDSLYGQDIDQASNLVLMQLSEGDQVWLETLRDWNGIYSSSEDDSTFSAKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZH7</accession>
    <name>A0A3B5KZH7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metalloendopeptidase</fullName>
            <ecNumber evidence="2">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1 2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZH7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003068.1">
        <property type="protein sequence ID" value="ENSXCOP00000003031.1"/>
        <property type="gene ID" value="ENSXCOG00000002393.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154856"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF899">
        <property type="entry name" value="ASTACIN-LIKE METALLOENDOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 2" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 2" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="3">
        <location>
            <begin position="56"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="252" mass="29360" checksum="FC140D580C1115B4" modified="2018-12-05" version="1">MKSLRIVAKLPKIQVALFTLDVWFAAGRIKQLSHYPKLETPELLDDIVIPDTRNRNADPCTARGCKWPKSGRFVNVPYKISSFFYRKFIMDALRSFHGRTCIRFVPKRPRHRDYIRFISGDGCWSFLGRQDGGQKLSLQKDGCLYDATVEHEVLHALGFDHEHVRSDRDKYIWIRYQNIKKGEKSNFDKKKTNNLGTPYDFKSVMQYSNDAFSKNGKPTIVAKRDPKMKFGDAKKLSVNDIARVNRLYKCSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L0P6</accession>
    <name>A0A3B5L0P6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Galanin peptides</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the galanin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027859180.1">
        <property type="nucleotide sequence ID" value="XM_028003379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L0P6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001379"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001402.1">
        <property type="protein sequence ID" value="ENSXCOP00000001379.1"/>
        <property type="gene ID" value="ENSXCOG00000001125.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSXCOT00000007288.1">
        <property type="protein sequence ID" value="ENSXCOP00000007196.1"/>
        <property type="gene ID" value="ENSXCOG00000005561.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114135791"/>
    <dbReference type="CTD" id="51083"/>
    <dbReference type="GeneTree" id="ENSGT00390000009663"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031763">
        <property type="term" value="F:galanin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008174">
        <property type="entry name" value="Galanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008175">
        <property type="entry name" value="Galanin_pre"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013068">
        <property type="entry name" value="GMAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16839">
        <property type="entry name" value="GALANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16839:SF1">
        <property type="entry name" value="GALANIN PEPTIDES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01296">
        <property type="entry name" value="Galanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06540">
        <property type="entry name" value="GMAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00273">
        <property type="entry name" value="GALANIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00071">
        <property type="entry name" value="Galanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00861">
        <property type="entry name" value="GALANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="6" id="KW-0372">Hormone</keyword>
    <keyword evidence="8" id="KW-0527">Neuropeptide</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044592470" description="Galanin peptides" evidence="9">
        <location>
            <begin position="28"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Galanin" evidence="10">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="118" mass="12746" checksum="08B0ADE143B86697" modified="2018-12-05" version="1">MQRCFGVFCVTLIFCATLSETIGLVIAAKEKRGWTLNSAGYLLGPHGIDGHRTLGDKAGLAGKRDMGQDEDFRTGSLRIGDEDIIHTVIDFLSYLKLKEMGALDSLSSPVSSDELANP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L3F0</accession>
    <name>A0A3B5L3F0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">nucleoside diphosphate phosphatase</fullName>
            <ecNumber evidence="3">3.6.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ENTPD6</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.6" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027862871.1">
        <property type="nucleotide sequence ID" value="XM_028007070.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027862872.1">
        <property type="nucleotide sequence ID" value="XM_028007071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L3F0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005337"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005398.1">
        <property type="protein sequence ID" value="ENSXCOP00000005337.1"/>
        <property type="gene ID" value="ENSXCOG00000004176.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114138047"/>
    <dbReference type="GeneTree" id="ENSGT01150000286963"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017110">
        <property type="term" value="F:nucleoside diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000052">
        <property type="entry name" value="Ectonucleoside triphosphate diphosphohydrolase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF99">
        <property type="entry name" value="ECTONUCLEOSIDE TRIPHOSPHATE DIPHOSPHOHYDROLASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01238">
        <property type="entry name" value="GDA1_CD39_NTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017325068" description="nucleoside diphosphate phosphatase" evidence="8">
        <location>
            <begin position="31"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="210"/>
            <end position="214"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="433" mass="47590" checksum="C33FEF13FFC721F4" modified="2018-12-05" version="1">MRFKMAALFLLLVLVLVLVLFLQRFSSSSSYRPPPHRPASHRTEPDWPGSSEGDGFQYEVMFDAGSTGTRVHVFRFQMGNPGSPSLDRETFRAIEPGLSTYADDPQKCSAGIVALLDLARSVVPPSQWTRTPVVLKATAGLRLLPGDRAEQLLDRVRALFQDSPFLSTEDSVSIMDGTDEGISAWITINFLTGGLQGLDRPTVGMLDLGGGSTQITFSPRHEKTIQTSAIDDIRSFQMFNRAHTVYTHSYLGLGLMSARLAVLGGVDVSPSGGSTERVSPCLAPEFSGSWEHADVIYTLKGQKEGEPVYEACLNKIQKVLYRKLSAAPEAADVDFYAFSFYYDRAVDLQAIDEAAGGTIRVSDYGQAAERVCRGPPDLQNPFLCLDLVYISVLLQELGFPAHKQLRLARTVRQVEASWALGAAFQHLEALRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5B1</accession>
    <name>A0A3B5L5B1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GNG2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">G proteins are composed of 3 units; alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <orientation evidence="1 9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the G protein gamma family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027856654.1">
        <property type="nucleotide sequence ID" value="XM_028000853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5B1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005026"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005087.1">
        <property type="protein sequence ID" value="ENSXCOP00000005026.1"/>
        <property type="gene ID" value="ENSXCOG00000003944.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114134327"/>
    <dbReference type="GeneTree" id="ENSGT01100000263497"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00068">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001770">
        <property type="entry name" value="G-protein_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13809">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00321">
        <property type="entry name" value="GPROTEING"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00224">
        <property type="entry name" value="GGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50058">
        <property type="entry name" value="G_PROTEIN_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="10">
        <location>
            <begin position="5"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="71" mass="7852" checksum="F450D02435B7A37A" modified="2018-12-05" version="1">MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMSYCEAHAKEDPLLSPVPASENPFREKKFFCAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6F3</accession>
    <name>A0A3B5L6F3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA repair protein SWI5 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Protein SAE3 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the SWI5-SFR1 complex, a complex required for double-strand break repair via homologous recombination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the swi5-sfr1 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SWI5/SAE3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6F3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006392"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006472.1">
        <property type="protein sequence ID" value="ENSXCOP00000006392.1"/>
        <property type="gene ID" value="ENSXCOG00000004951.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009349"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032798">
        <property type="term" value="C:Swi5-Sfr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034974">
        <property type="term" value="C:Swi5-Swi2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000056">
        <property type="entry name" value="DNA repair protein SWI5 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010760">
        <property type="entry name" value="DNA-repair_Swi5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28529">
        <property type="entry name" value="DNA REPAIR PROTEIN SWI5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28529:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN SWI5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07061">
        <property type="entry name" value="Swi5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00059338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="97" mass="11054" checksum="E874C2C95CF42384" modified="2018-12-05" version="1">KKVNSHFKSPLQVPDSPKVSPEEEVAELQKKKEQLDSEIAQLEEEGCRVEELDQHIDKLHEYNDIKDIGQSLLGRIAALRGTTTRDLYAHFGLELDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6G0</accession>
    <name>A0A3B5L6G0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">COPII-coated vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6G0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006005"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006073.1">
        <property type="protein sequence ID" value="ENSXCOP00000006005.1"/>
        <property type="gene ID" value="ENSXCOG00000004660.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006916"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF7">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="2">
        <location>
            <begin position="58"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="115" mass="13075" checksum="68A05DC072282BAF" modified="2018-12-05" version="1">MATFAEYIAQNEERDGVRFSWNVWPSSRLEATRMVVPVAALFTPLRERSDLPPIQYEPVLCSRATCRAVLNPLCQVDYRAKLWACNFCYQRNQVSGVLLPLSCCLTSPPSSMLCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6L1</accession>
    <name>A0A3B5L6L1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cyclic nucleotide gated channel subunit alpha 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6L1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006462"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006542.1">
        <property type="protein sequence ID" value="ENSXCOP00000006462.1"/>
        <property type="gene ID" value="ENSXCOG00000004999.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156074"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017071">
        <property type="term" value="C:intracellular cyclic nucleotide activated cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005222">
        <property type="term" value="F:intracellularly cAMP-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005223">
        <property type="term" value="F:intracellularly cGMP-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000002">
        <property type="entry name" value="Cyclic nucleotide gated channel alpha 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000001">
        <property type="entry name" value="Cyclic nucleotide-gated channel alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyclic nucleotide-gated channel alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032406">
        <property type="entry name" value="CLZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050866">
        <property type="entry name" value="CNG_cation_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638:SF20">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CHANNEL SUBUNIT ALPHA 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16526">
        <property type="entry name" value="CLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="12">
        <location>
            <begin position="386"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="572"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="16"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="575"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="586"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="642"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
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            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="658" mass="76581" checksum="38E6CFEEF63B298A" modified="2018-12-05" version="1">MVFVHFTESCRKKKKEKEEKEAKEKEQEREKEKEKEKEREKEKEKEKAKEKDKDKPKEIFVINPAGNLYYNWLFIIAMPVMYNWTMIIVRACFEELQQDYILYWVLLDCSSDLIYLADMFVRTRTGYLEQGLMVKDKKLLRDNYVKSFQFRLDVLSMLPTDLLYFVFGLHYPEIRINKLLRIGRMMEFFTRTETKTNYPNIFRIANLIMYILIIIHWNACFYFSFSKSIGFGADDWVYPALDDPEEPEFGQPMRKYAFSLYWSTLTLTTIGETPPPALDSEFLFHVVDFLVGVLIFATIVGNIATMISNMNAAQAQFQSRIDNIKQYMQVRKVSKELELRVIKWFDYLWNNGKAQDEREVLRYLPDKLRAEIAIQVHMDTLKKVRIFADCEAGLLIQLVLKLCPQVFSPGDYICKKGDIGREMYIIKDGKLAVVADDGVTQFVVLGSGSYFGEISILNIKGSKAGNRRTANIRSIGYSDLFCLSKDDLMESLIEYPDAKAMLEEKGRQILMKDGLIDLDPANIKPEVKELEEKVTKLYSTMELMQVKLKKILGNYKNTGKALRQRITDLERLSGEEVEEEEEEEDEGKKEAEEKEAEKEPDEKVVEEKEEKAEEEKGVDEKDVVETEEKTEGKGAGEKVMEEAEEITDEDKSEVTKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L780</accession>
    <name>A0A3B5L780_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ninjurin 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ninjurin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L780"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006372.1">
        <property type="protein sequence ID" value="ENSXCOP00000006295.1"/>
        <property type="gene ID" value="ENSXCOG00000004867.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158892"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042246">
        <property type="term" value="P:tissue regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007007">
        <property type="entry name" value="Ninjurin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12316:SF19">
        <property type="entry name" value="NINJURIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12316">
        <property type="entry name" value="NINJURIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04923">
        <property type="entry name" value="Ninjurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="142" mass="16019" checksum="2A52C1CF2F26B2CA" modified="2018-12-05" version="1">MDTETIAMNGDVFHRENRIVRRRGPLNMNHYANKKSAAESMLDVALLMANASQLKAVLEHGPSFTFYVPLITLLSISLILQIVVGVMLIFIVKWNLNDERMHHKLDILESCVMAFIFVIVVVNVFITAFGVQQPHSRDSPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7Q3</accession>
    <name>A0A3B5L7Q3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Receptor class 5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006470"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006550.1">
        <property type="protein sequence ID" value="ENSXCOP00000006470.1"/>
        <property type="gene ID" value="ENSXCOG00000005006.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155048"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17670">
        <property type="entry name" value="R-PTP-G-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000016">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase gamma isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000255">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase gamma isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000013">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase zeta isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF468">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="633"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="19">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="255"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="16">
        <location>
            <begin position="717"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="17">
        <location>
            <begin position="908"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="16">
        <location>
            <begin position="1019"/>
            <end position="1279"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="17">
        <location>
            <begin position="1196"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="437"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="440"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="472"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="485"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="506"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="558"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="589"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1314" mass="147445" checksum="4BE35C39FC3CABE6" modified="2018-12-05" version="1">TVVYSVESASECVLEYLSLIQFLHPSLSPVAVLLKDDYFVRGAGLPGRFKAEKMEFHWGQSNGSAGSEHSINGRRFPVEMQIYLYNSDDFDSLNAAMKERRIIAAMAVFFELGQKDNPAVDPIIQGLKGVVHHEKETNLRSFILRDLLPSSVDSYYRYTGSLTTPPCSKVVEWIIFSRPVYLSHSQLDAFYSIFTTEQQDHVKSVEYLTNNFRPLQDLNNRKIFKSAVKDAWQKDLTEILGSPHSTEASRVCSSAPVSMKIQPLNETALMVSWERPLTIYHPPITSYMVSYSWVKRDVADEKTFLKTGDQSMKAVITNVSPDLLYLFRVQAVCQHDLRSDFSQTLLFRANTTRIFEGSRIVKTGMPTISPASSADMAPISSGSSTWTSSGIHFSLVSMATGMGPSSSGSQATVASVVTSTLLAGLGVGGGVISSLPSSVWPTQTPPASQNPTHPSTPPSKPSTEQAAGQGAESEKQAEDKPAAGEGEEKEEENGEKEEEEEEEEELEKRKKDKNSPDNEEKQANSTVSKEPLIPTPASTAKEKGKGNSTADAEPASSGDDRLVNEEKRPTEVNTLSTQEPHNDSAEMQIPQSSTVSPKNRSDGKGQWPFSVHTDQTILSNVTRTPPHPGMGRMVWIVPLVVVSALTLLCLVMLLIVMVYWRRFFQTAHFYVEESSSPRVVANENIPIIPIPDDMDAIPVKQFVKHVMELYKSNMQGFAEEFEEVQRSTVDLKITAEHSNHPDNKHKNRYINIVAYDHSRVKLRALAGKDAKHSDYINANYVDGYNRPRAYIAAQGPLKSTFEDFWRMVWEQNTGIIVMITNLVEKGRRKCDQYWPTESSEQYGNIVVTLKSTKVHACYTLRRFLIRNTKVKKGQKGNQKGKLNERIVIQYHYTQWPDMGVPEYTLPVLTFINRSSAARTADMGPVLVHCSAGVGRTGTYIVIDSMLQQIKDKSTASVLDFLKHIRTQRNYLVQTEEQYVFIHDALMEAILSRETEVPAWKLHGYVNSILTPNSTGRTRLEKQFRLLSQCNTRFVECFSAHKDCNKEKNRNSSVVPSERARVGLTTLPGMKGTDYINASYIMGYYRSNEFIITQHPLPHTTTDFWRMIWDHNAQIIVMLPDNQGLAEDEFVYWPSREEAMNCIAFTVTLISKDRLCLSNEEQIIIHDFILEATQDDYVLEVRHFQCPKWPNPDAPLSSTFELINVIKEEAMTRDGPTIVHDECGAVSAGMLCALTTLSQQLESEGVVDIYQVAKMINLMRPGVFTDIEQYQYLYKAMLSLVSNRECSLSPTHTDTNGVVVIADESDPAESMESLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LA18</accession>
    <name>A0A3B5LA18_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="1">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="34 35">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="23">Postsynaptic cell membrane</location>
            <topology evidence="23">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="28">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="1"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
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            <begin position="486"/>
            <end position="512"/>
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            <begin position="311"/>
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        <location>
            <begin position="570"/>
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        <location>
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        <location>
            <begin position="577"/>
            <end position="584"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        <location>
            <position position="604"/>
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        <location>
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        </location>
        <ligand>
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        </ligand>
    </feature>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="863" mass="96283" checksum="247529A0D1BCC1EF" modified="2018-12-05" version="1">MCESPVLMNLDRCVTECFLFLFFPYWLAPRIITLLETVDVLLDHNATFICEVESSPSASITWTKNNQPIMYYDNRYLTKEQGQMLTIPHVKDSDNGEYCCIASNGIGEAAKSCGALQLKMKPQIKRQPTNVTLLIESKAVLPCVTLGHPKPDVTWLKDDELIKISDRVTILDYGALKIHRIQTEDAGMYRCVARNSFGLAFSKPVTIEVQTPARLLRVPKEKRADYGSQITLECNATGNPTPTITWLENGNTVSGASVEETLEGNVILSVLKVTVTKSALYTCVASNRHSGGANNVKKSARVTEWSSSHKGITGYCSAYRGDACRTNLRDDSLVFFNSSLSDPEGMQEYLVQSWLAELEGLSGLCNPAALSLLCHSAFPDCNPSGGSAPKPVCREHCLAVKELYCHKEWLTLEGTGSVQAGLLSSVTRSQIFTSLLPNCQALPSLHTDPYGCTYIPFVVENLTNFSSFSPDQVPRPPPTSTSSSPAYSMTVIVIVLTSMAIAVFLTIIILGCHRRRKQWRNRKREIGTPTLSALPSELLLDRLHPNPMYQRVPLLLNSKLLALEYPRNNIQYVRDIGEGAFGRVFQARAPGLLPTEPFTMVAVKMLKEEASADMQNDFQREAALMAEFDHSNIVRLLGVCAVGKPMCLLFEYMAQGDLNEFLRRRSPTQSVRTISRASLSARSFSSDGEAASLTCPEQLSVSKQIAAGMAYLSERKFVHRDLATRNCLVGEEMIVKIADFGLSRNIYSADYYKANENDAIPIRWMPPESIFYNRYTTESDVWAYGVVLWEIFSHGMQPYYGMGHEEVIYYVRDGHILSCPEDCPLELYNLMRLCWSTHPSDRPSFSSIHRILERITVIQEGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAH4</accession>
    <name>A0A3B5LAH4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Solute carrier organic anion transporter family member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the organo anion transporter (TC 2.A.60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAH4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007837"/>
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        <property type="protein sequence ID" value="ENSXCOP00000007837.1"/>
        <property type="gene ID" value="ENSXCOG00000006019.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286901"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015132">
        <property type="term" value="F:prostaglandin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015347">
        <property type="term" value="F:sodium-independent organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004156">
        <property type="entry name" value="OATP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00805">
        <property type="entry name" value="oat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388">
        <property type="entry name" value="ORGANIC ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388:SF14">
        <property type="entry name" value="SOLUTE CARRIER ORGANIC ANION TRANSPORTER FAMILY MEMBER 2A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03137">
        <property type="entry name" value="OATP"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="156"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="359" mass="40519" checksum="8A74DAF24B20AA60" modified="2018-12-05" version="1">YFRSTISTIERRYGLSSFSSGTISSLNEISNCILIVFVSYFGSRVHRPRVIGFGGLLMAISAMILTLPHFLSQPYTRDLSSQSKSKLHFHDLCKPHDNTTSPESCDQEDTRRLADTTQVWILMAIAQLLFGVGSVPIQPFGISYIDDYAGSKNSPLYIAILFAVSVFGPSIGYLLGSIMLRFYVDVNRNAFPVLSPVDPEWVGAWWMGLLIITVFLTLSSIPYFFFPRQMNFEVNVSDYDKTGCAYSIAMMDIDLARSLQASFLFLYVLWTVFPRMFFRLLSGPLFLLLVLSQCCFSSVIAGLASFLSKFLERQYGASVTYAEHPSHGVSFRVETRNLNTSNKDTYMFFPHCLNLNQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBM0</accession>
    <name>A0A3B5LBM0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBM0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007106"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007196.1">
        <property type="protein sequence ID" value="ENSXCOP00000007106.1"/>
        <property type="gene ID" value="ENSXCOG00000005496.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00960000191276"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="114" mass="12559" checksum="BCCD850FAC65E404" modified="2018-12-05" version="1">MLCSIYPHEMYCLSGQVPFGLKLHPDRKHLIYPLGSIIVLKRIEDGKLEFLDGHSNNVSCISVSKSGSYIASGQVNFMGFQVATLTSLLKNTWCLLGAKMTAGKQQSLTRLKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LC05</accession>
    <name>A0A3B5LC05_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Signal transducer and activator of transcription</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the transcription factor STAT family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LC05"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008377"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008483.1">
        <property type="protein sequence ID" value="ENSXCOP00000008377.1"/>
        <property type="gene ID" value="ENSXCOG00000006422.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01080000257420"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002432">
        <property type="term" value="P:granuloma formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000126">
        <property type="entry name" value="Signal transducer and activator of transcription"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000029">
        <property type="entry name" value="Signal transducer and transcription activator 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.630:FF:000003">
        <property type="entry name" value="Signal transducer and transcription activator 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.20">
        <property type="entry name" value="STAT transcription factor, all-alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.630">
        <property type="entry name" value="STAT transcription factor, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.532.10">
        <property type="entry name" value="STAT transcription factor, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001217">
        <property type="entry name" value="STAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048988">
        <property type="entry name" value="STAT_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036535">
        <property type="entry name" value="STAT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015988">
        <property type="entry name" value="STAT_TF_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013801">
        <property type="entry name" value="STAT_TF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012345">
        <property type="entry name" value="STAT_TF_DNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013799">
        <property type="entry name" value="STAT_TF_prot_interaction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11801">
        <property type="entry name" value="SIGNAL TRANSDUCER AND ACTIVATOR OF TRANSCRIPTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02864">
        <property type="entry name" value="STAT_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02865">
        <property type="entry name" value="STAT_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21354">
        <property type="entry name" value="STAT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00964">
        <property type="entry name" value="STAT_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47655">
        <property type="entry name" value="STAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48092">
        <property type="entry name" value="Transcription factor STAT-4 N-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0010">Activator</keyword>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12 13" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="10 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 13" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SH2" evidence="14">
        <location>
            <begin position="522"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU046415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="681" mass="79182" checksum="07F155F5058454A5" modified="2018-12-05" version="1">MAQWLEMSYVLTNMDDERINALYPPDTFPIEIRHYLCQWMEEKDWEGFNPDQSDKEQLAQALLSDCILLLESVAEQQNIVERMKLLQITKNLMTRPAMEFAVKIRNILRQEKMILCERHTPKRTEGKTPDFEDLTFMKSIVLQLQDRRKRIHSCKENMNWEMVNYEALQGKELQMLSKKIQQLKINQKTLTKLKQCQDNFIIKMNVWQWEQHKSVIGYPFTDDLSHMQNWSEQLFAVNSNLRQEWLLIPDLPKEIGEKLTCLLQELIQSSFVVEKQPPQVIKTQSKFSAVVRYLLGESLVSGQPVTMKAQIINELQARNVNLAFDNVGELINHTAILDRNTATKTTNANFRHMSIKKIKRADRKGAESVTEEKFALMFLAEIFITGHETPFKIHINSQPVVVIVHGSQDINAVATIIWDCAFAEPDRVPFVVPDRVTWKMMIETLNVKFCSEVGTNHNLNPYNMHFLAQKIFDQPEYASDLSDSVVTWCQFNKEVLAGRNFTFWQWFEGAMDLTKKHLKPYWSDGLIFGFIGKQHVHLILKESPSGTFLLRFSDSEIGGITIAYVSHENGIPMIQNIQPFTRKDLEIRCLGDRIRDINEILYLYPNVLKHDAFKKFYTEQQPTNSGYIPVRLQTKVGTGMLFNVKSCIRLRKTLIMCFLSSSPADGMDYDFYFNDFPKNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCL5</accession>
    <name>A0A3B5LCL5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Antifreeze protein type IV</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCL5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005675.1">
        <property type="protein sequence ID" value="ENSXCOP00000005609.1"/>
        <property type="gene ID" value="ENSXCOG00000004370.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00760000119652"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042157">
        <property type="term" value="P:lipoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006801">
        <property type="entry name" value="ApoA-II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04711">
        <property type="entry name" value="ApoA-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58113">
        <property type="entry name" value="Apolipoprotein A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017199680" evidence="1">
        <location>
            <begin position="19"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="148" mass="16759" checksum="93FB1F4DBBFEF202" modified="2018-12-05" version="1">MKFSLVATILVVLAVSEARSLVKREVQAEVDRITQILREMSASVTMATQDMVEKMKALEMTNTATYMEDSRAKIQPLVEQVQSEAAKLQEQVKPLISNIEEHIRPLTDDFNAQVLTDNLQNQVKPLTSMMEKFLQQVMEQSKALLPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCN9</accession>
    <name>A0A3B5LCN9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCN9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008718.1">
        <property type="protein sequence ID" value="ENSXCOP00000008612.1"/>
        <property type="gene ID" value="ENSXCOG00000006590.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163711"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050488">
        <property type="entry name" value="Ig_Fc_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481:SF64">
        <property type="entry name" value="FC RECEPTOR-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481">
        <property type="entry name" value="IMMUNOGLOBULIN FC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="3"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="209"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="317" mass="35261" checksum="515EA2CD59623AF3" modified="2018-12-05" version="1">PRPAVSLTSVLTVSPSWPSPGASVTLTCNHDDQSAGWRFYWYKAAPHKSEDGYRYELLPDSISGTVQDSFIIHEQNHTSGYSCRAGRRNPELFTDYSEPKFVWSAVSRPAASLSVSPDREQHFTSESVTLNCGGNSAEWRVRRFSESGYLIVIANCYYWGKMSGSSCIVKYLSNPKSVYWCESGSGEFSNAANITSHSTLLSNVPVHPVTEGASVTLGCRLRGENQLSNVFFYHNDKLIQSDSRGELNISAVSQSDEGFYKCEHSGKVSPQSWMAVKGEQRFNTLLHFCGSVLVILLLLLCCFRLSKGETFHLLKLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCX8</accession>
    <name>A0A3B5LCX8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027872081.1">
        <property type="nucleotide sequence ID" value="XM_028016280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCX8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008496.1">
        <property type="protein sequence ID" value="ENSXCOP00000008390.1"/>
        <property type="gene ID" value="ENSXCOG00000006435.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114143894"/>
    <dbReference type="KEGG" id="xco:114143894"/>
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    <dbReference type="GeneTree" id="ENSGT00940000155969"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Complement C1q B chain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cerebellin_Synaptic_Org"/>
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    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923">
        <property type="entry name" value="CEREBELLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923:SF99">
        <property type="entry name" value="COMPLEMENT C1Q-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017418524" description="C1q domain-containing protein" evidence="7">
        <location>
            <begin position="19"/>
            <end position="241"/>
        </location>
    </feature>
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        <location>
            <begin position="108"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="48"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="241" mass="25548" checksum="91A809F40FA163F7" modified="2018-12-05" version="1">MVLILLVAIPLLVHTTKGGSAGATGSRYEMLGSCRMVCDPYATSQPGQELIAVSPPPQEYSGKKIRSGLRGPPGNPGPPGERGPPGEPGKPGPQGPPGPGPGGYSPSIYTPKIAFYAGLRKQHEGSEILKFDDVVTNVGNYYEPSTGKFTCPLPGIYFFTYHVLMRGGDGTSMWADLKKNGLVRASAIAQDADQNYDYASNSVILHLDVGDEICVQLDGGKVHGGNQNKYSTFSGFLIYPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LE62</accession>
    <name>A0A3B5LE62_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LE62"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009243.1">
        <property type="protein sequence ID" value="ENSXCOP00000009132.1"/>
        <property type="gene ID" value="ENSXCOG00000006968.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050822">
        <property type="entry name" value="Cerebellin_Synaptic_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923:SF102">
        <property type="entry name" value="CEREBELLIN 13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923">
        <property type="entry name" value="CEREBELLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017362946" description="C1q domain-containing protein" evidence="4">
        <location>
            <begin position="22"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="5">
        <location>
            <begin position="104"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="243" mass="26863" checksum="4BBC9309ECA156DF" modified="2018-12-05" version="1">MFFRALLLICSLSSAQLHAAAENKIRQTGSQEGDAENLSNNQQTFTQDINAVLREMSASLAGLKVELRCLQKDHDVLICLIFQTPDSFLRTSNGLIPIKLSYCLVVKQVAFSASLLASGDGHIGPFNTQTPLVFRHVVANIGNAYNPNTGFFIAPMRGAYHFEFHLHGLGHASYPTGAVLVKNGDPIFTAYEHQASYYVNPSNGATLLLETGDVVFLRQWQNTRIYDNHNHHTTFSGHLLFTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LF21</accession>
    <name>A0A3B5LF21_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Zgc:154006</fullName>
        </submittedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the ELL/occludin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LF21"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009548.1">
        <property type="protein sequence ID" value="ENSXCOP00000009437.1"/>
        <property type="gene ID" value="ENSXCOG00000007189.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167296"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031176">
        <property type="entry name" value="ELL/occludin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010844">
        <property type="entry name" value="Occludin_ELL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288:SF38">
        <property type="entry name" value="OCCLUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288">
        <property type="entry name" value="OCCLUDIN AND RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07303">
        <property type="entry name" value="Occludin_ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144292">
        <property type="entry name" value="occludin/ELL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51980">
        <property type="entry name" value="OCEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10 11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6 11 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="169"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="252"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="15">
        <location>
            <begin position="63"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="OCEL" evidence="16">
        <location>
            <begin position="415"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="356"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="492" mass="55495" checksum="6D101DF040145A62" modified="2018-12-05" version="1">MYEPRYYDSPPVYSPPYSTTSHSLYLPRSVHSPQSQYVSYAHQPPPDSYYMEGQPQHFYRWFSPPGFVKTFQGTTALMCFIIFACVASTLVWDMNGFGYGGYGVGDAVSVGGAGSGYYGGSYGYGGSYMTPQSAKAAMISMVAINFLVSLSFLVGSFSRSRMMRGCRFYLTIFISDIILAVFQGIIDIIFVIGVNPMSQSSQSMLYNPMLRMCQNIQGTPSLSGSVGAGFPGGFPLYNQYLYHYCFMDPEEAVGLVLGLLVVVALSISAYYAYKTRSKIWRHGKSNILWDEPMVRLSDGHDVLDWVSVLCENVSLIADFVFLVSSCTENSARHDTEPVYQNTRATVCSSSSYETDSVRKLPLHHTNKREPPLHHNNKRPQKQLTEPTPAAQLAAESQYETGYTSGDTGNELDWDQRDYLMYPAITTDEQRRQYKREFDSHLAHYKRLCSEMDHISDQVHKLSRELDTLDEGSVRYQGVADEYNWLKDLKKVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFE0</accession>
    <name>A0A3B5LFE0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the L6 tetraspanin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFE0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010873"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010998.1">
        <property type="protein sequence ID" value="ENSXCOP00000010873.1"/>
        <property type="gene ID" value="ENSXCOG00000008212.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234590"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008661">
        <property type="entry name" value="L6_membrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14198:SF18">
        <property type="entry name" value="TRANSMEMBRANE 4 L6 FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14198">
        <property type="entry name" value="TRANSMEMBRANE 4 L6 FAMILY MEMBER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05805">
        <property type="entry name" value="L6_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="161" mass="17424" checksum="013467502B7EEE4A" modified="2018-12-05" version="1">CSVGFARSLGLALLPLALCSILANLLLLFPMGEITYIQQDRLASYIWYFGGLGGGGLLMCGSVLAAIVGLLGSGYCFVISGLALVQGPHCFTSYGWYLLQPEIWPRCLQPINIVEWNITLLCVLLGLAVLEFIICLLQLGNGLVNAICRPCCYKQEYSLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFZ3</accession>
    <name>A0A3B5LFZ3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFZ3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011058"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011185.1">
        <property type="protein sequence ID" value="ENSXCOP00000011058.1"/>
        <property type="gene ID" value="ENSXCOG00000008356.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158589"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:proteolysis"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
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    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF27">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S1" evidence="3">
        <location>
            <begin position="25"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="107" mass="11775" checksum="3ABB4C95FFE68548" modified="2018-12-05" version="1">LARFALLPVLELRGFFPRSTALMSIVGGNISKPGQFPWQVSLHFQREHICGGSIITSSWVLTAAHCVYGSDRCAPPLLAPTHHKVVVSGQLHIALHPSVQDTWLQIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LG48</accession>
    <name>A0A3B5LG48_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PAR14-like first RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LG48"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009565"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009677.1">
        <property type="protein sequence ID" value="ENSXCOP00000009565.1"/>
        <property type="gene ID" value="ENSXCOG00000007286.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177519"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057051">
        <property type="entry name" value="PARP14_RPM_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23222">
        <property type="entry name" value="RRM_PARP14_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAR14-like first RRM" evidence="1">
        <location>
            <begin position="13"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="109" mass="12585" checksum="99112A235796D231" modified="2018-12-05" version="1">YRGQTGPEYTFHVLVELEETNTPRLKNKLVKYFQSKKSNGGDCEVDYESGSSTALLRFQEGESQTMTVRLPSDGKQKEVRAKKWKLVNNLTHRQVPEHPVVKVFEQLFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGD7</accession>
    <name>A0A3B5LGD7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027896792.1">
        <property type="nucleotide sequence ID" value="XM_028040991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGD7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010035.1">
        <property type="protein sequence ID" value="ENSXCOP00000009917.1"/>
        <property type="gene ID" value="ENSXCOG00000007526.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114158994"/>
    <dbReference type="KEGG" id="xco:114158994"/>
    <dbReference type="GeneTree" id="ENSGT00950000182966"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:external side of plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:leukotriene receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Leukotriene B4 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF946">
        <property type="entry name" value="LEUKOTRIENE B4 RECEPTOR 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="45"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="320" mass="36051" checksum="909F88187201B28D" modified="2018-12-05" version="1">MELFNSTVVTYNLSSSGLLQHPSWLSKGVVPVVVVSFCCIVGVSGNIAVILLKPNWHHLSSLSQSLMLNLAISDLLCLLTLLPWIYPLLYGWSFGLVACKILTYLVYCSIYGSKLTVIVLSIQRYLTVVHQRRFSQVKKRAMLALLWMAAVILSVPALVVRQLSTNQQWIVCQSQYSSMTQGVVVLLAETLFDFCSFALVVFSYVRLHRTVNQAAFFNNPQTTRLITSIIVSYFVLWTPYHIINVLGVAGMCLKNERLLKFCKDTGYVVKTLTFVHSCLNPLLYAFTSNKMCVVCQKREEVQSQSLQTNHSPDIGRVLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHS8</accession>
    <name>A0A3B5LHS8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHS8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010412"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010535.1">
        <property type="protein sequence ID" value="ENSXCOP00000010412.1"/>
        <property type="gene ID" value="ENSXCOG00000007888.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000203785"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="215" mass="23528" checksum="18465E27EAFAD4FA" modified="2018-12-05" version="1">PWRRSYGESHRLQLRVRPFPPGPSRGLVFPGKSLFGFIRRVYRCCQEGVGCRSVKGIQTAGVEFVLTREILAATASRAELHLQLSNPRRLDISPVIPFMAKRNLPTRYSVASRGDAVELRVDLLFLLRLLQEAAGDGGRGLVNEHLGRLLSSEDGETSLGIVQDADGEAWHDRKDVILARDVGLVLGCSRAGAGVPCESGGVRLSDAPFMAVYYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIF8</accession>
    <name>A0A3B5LIF8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TLC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIF8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010548.1">
        <property type="protein sequence ID" value="ENSXCOP00000010425.1"/>
        <property type="gene ID" value="ENSXCOG00000007898.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01010000222313"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071709">
        <property type="term" value="P:membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055091">
        <property type="term" value="P:phospholipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097035">
        <property type="term" value="P:regulation of membrane lipid distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050846">
        <property type="entry name" value="TLCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13439:SF2">
        <property type="entry name" value="TLC DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017203206" description="TLC domain-containing protein" evidence="7">
        <location>
            <begin position="21"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="8">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="168" mass="18975" checksum="34D76BA544A9C898" modified="2018-12-05" version="1">MHPVFGFSFLCFFLNPFHFAAPWSATHVNLVSKNAQASLPSVVITCFGLSVVSCRYVGFSVVALLVEINSVFLHLRQILRMASMAASTLYRVNSIINLGTYVVFRINTLAWMTRWLVLNRDKIPLLAYTLGSVGMAIMTVMNIILFCRLLRSDFLKSSTREAKKEKEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIV0</accession>
    <name>A0A3B5LIV0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIV0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010570"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010694.1">
        <property type="protein sequence ID" value="ENSXCOP00000010570.1"/>
        <property type="gene ID" value="ENSXCOG00000007995.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156836"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF491">
        <property type="entry name" value="KELCH REPEAT AND BTB DOMAIN-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="395" mass="44158" checksum="27075581B79D56C8" modified="2018-12-05" version="1">LRRVRLELVDPGFLRKARRRNPVLLRDAECFGMIDAALQTSGLCETSAPPRPPLRYGMETTDLLLCLGGVNTEGVPARRGGLADQSFCFAPHGRKTYYIPSPLRDCGGQGQVTAGAVTRDSSILVAVEAEDQHRRKRLDIYNTSEEKIWVKLCSAPYRDMYALGLVGDALYLIGGQMKVRNHYIITDSVNRWSLKRGGSWLSFAPLPLALACHYTVSLKERLYVLGGWTPQDQPDDEPDKLSNRVFRFDPGKDRWTECARMKYSRYRCGTAVLNGEIYILGGIGCDGEDCGQSRRCLSSVEIYNPDADSWRPGPTLPTTLLSLRTNASNIGTVEGKLYLCGYYKGVGRHEIITKEILELDPADYVWTVVERRAAMHDSYDVCLVANLNPRDLLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LL12</accession>
    <name>A0A3B5LL12_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Jacalin-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LL12"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008564"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008670.1">
        <property type="protein sequence ID" value="ENSXCOP00000008564.1"/>
        <property type="gene ID" value="ENSXCOG00000006554.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164478"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.30">
        <property type="entry name" value="Jacalin-like lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036404">
        <property type="entry name" value="Jacalin-like_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052321">
        <property type="entry name" value="PolyBind_ProtTraffic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33589">
        <property type="entry name" value="OS11G0524900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33589:SF3">
        <property type="entry name" value="ZYMOGEN GRANULE MEMBRANE PROTEIN 16-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00915">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51101">
        <property type="entry name" value="Mannose-binding lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51752">
        <property type="entry name" value="JACALIN_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="3">
        <location>
            <begin position="15"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="157" mass="17398" checksum="2860483AC198EA6C" modified="2018-12-05" version="1">LVTFLFFPIPVRPHYSFSPPIGSGGGNSYAITGQGRITAVRLFTLFLFFSSIQFQYRYLSSSGVGYTYGDAQELQLYDGEAVIQISGKHSSYLQSIVFTTNRSRSLYVGQPSGTSFNMYPDRPEAELIFISSHVSGGLTSLGSHWAIVYSYKILFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LL53</accession>
    <name>A0A3B5LL53_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Elongation factor RNA polymerase II</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ELL/occludin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LL53"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012818"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012972.1">
        <property type="protein sequence ID" value="ENSXCOP00000012818.1"/>
        <property type="gene ID" value="ENSXCOG00000009675.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155914"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2670">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042065">
        <property type="entry name" value="E3_ELL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031176">
        <property type="entry name" value="ELL/occludin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019464">
        <property type="entry name" value="ELL_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010844">
        <property type="entry name" value="Occludin_ELL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288">
        <property type="entry name" value="OCCLUDIN AND RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288:SF9">
        <property type="entry name" value="RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10390">
        <property type="entry name" value="ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07303">
        <property type="entry name" value="Occludin_ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144292">
        <property type="entry name" value="occludin/ELL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51980">
        <property type="entry name" value="OCEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="OCEL" evidence="8">
        <location>
            <begin position="502"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="165"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="285"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="343"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="360"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="389"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="454"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="465"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="612" mass="67726" checksum="0E7540956AC56DE1" modified="2018-12-05" version="1">MAALKEEQCYGLSCGRVSNGSNVTVFHVKLTDSALRALEGFRSSKILSQQPFIRFNGNQGKISIPRSETSSELQTFTFDLSNVGRDNPQGSFDCIQQYITSEGGIQLDCLGSIQDKITVRATDDSYQKARESMAQAEETTRSRSAIVIKPGGRFVGKKVQIRKPAPALSDVAPSRRTSRPVLISSSTQKKATTQHRPLRERLVHLLALKPYKKPELILRLQKDGLSQLDKESLDSHLQQVANLNGKENTFTLKDFMYKEVQKDWPGYTEGDQQLLKRILFRKQAQTSSAPVPESPPKELGSSSPSQVSPKRPTVDFIDPLANKKPRISHLASKAATVQMNGKLGSSCGRGDPGAALSGAAVSTSESGMTSGSQPLPVLDIPRPFEALSDVSNDSSNNGRDGDSQETTLSERLSQPPSLLSVSTVASAPSLGTSSSGHTVLEGPRDNGPSAVNNKSRKKSKKHKDKEKSKDKGKGRAKEKKICQEPASEPKTTCEMSLKIFKNSVAFKSTVITSPEQRQRYKNDFNAEYSEYRGLHARIEGITRQFTVLDNELKQLQQGTDKYKSIHNQILQEYHKIKKTNPNYSQEKNRCEYLHNKLAHIKKLIAEYDHQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLZ5</accession>
    <name>A0A3B5LLZ5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Potassium sodium-activated channel subfamily T member 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLZ5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013248.1">
        <property type="protein sequence ID" value="ENSXCOP00000013088.1"/>
        <property type="gene ID" value="ENSXCOG00000009915.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156880"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005228">
        <property type="term" value="F:intracellular sodium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000011">
        <property type="entry name" value="Potassium channel subfamily T member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000069">
        <property type="entry name" value="Potassium sodium-activated channel subfamily T member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF14">
        <property type="entry name" value="POTASSIUM CHANNEL SUBFAMILY T MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="187"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="13">
        <location>
            <begin position="257"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="426" mass="48898" checksum="D01BF70C8C30492C" modified="2018-12-05" version="1">NTFKERLKLFFIKNQRSSLRIRLFNFALKILTCALYILRVSLDNPTENNSINGCQVHSSPSPSVLQVTVALISFLETMLITYLSYKGNIWEQIFQISFILEMINTVPFIITIFWAPLRNIFVPVFLNCWLAKGALENMINDFHRAIQRTHSAMFNQVFILICTLLCLVFTGACGIQHLERAGKNLSLFDSFYFCIVTFSTVGYGDVTPQIWPSQLLVVILICVALVVLPLQFEELAYLWMESQKLGGNYSRHRAQTEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEIDIQVRRILQIPLWSQRVIYLQGSALKDQDLMRAKMDDAEACFILSSRNEVDRTAADHQTILRAWAAKDFAPNCPLYVQILKPENKFHVKFADHVVCEEEFKYAMLALNCVCPATSTLVTLLVHTSRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNN3</accession>
    <name>A0A3B5LNN3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNN3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012340"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012489.1">
        <property type="protein sequence ID" value="ENSXCOP00000012340.1"/>
        <property type="gene ID" value="ENSXCOG00000009327.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155181"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031635">
        <property type="entry name" value="NTRK_LRRCT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16920">
        <property type="entry name" value="LRRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017244571" description="Ig-like domain-containing protein" evidence="4">
        <location>
            <begin position="39"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="177"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="441" mass="49244" checksum="9029D9CC6E0C78B7" modified="2018-12-05" version="1">MCASGPGIWALPSAWGTSGSWQCFWWWVVAVGVTVGSGDPWMSAEACPSTCSCSSTRISCVDPERGISAFPVLQSEAEMENITDIYIANQSSFSSINDKDLHYYKNLRNLLCQSSSPCVCTYSSRILSGNPLHCSCENMWIKLWLGEEADTQELRCIEDGGARKPLSRLRLPKCEVPMATLSPSKVTKMEKDNVELFCTTSGMPSPELIWDMALVTNYIETSGQISVLRLSNLSSMDHNKKISCTAENIVGERESSLLLDILFAPQITKLSDAIADHHWCIPFSVAGMEDNLIKNILTEGEYIMTMIHDITEREYHGCLQLDNPTHINNGLYRLEVKNKYGSDEKEITAHFMRRPDDGEEQYWNLFCLVRGACSLSEGAHKIITVPDPHEHNHTLNKSHVCAITVLNVCRNVLIISIKIAMKKSYRLQSIEAAALPAIERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LP24</accession>
    <name>A0A3B5LP24_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glomulin, FKBP associated protein a</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LP24"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009599"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009712.1">
        <property type="protein sequence ID" value="ENSXCOP00000009599.1"/>
        <property type="gene ID" value="ENSXCOG00000007309.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018446"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055105">
        <property type="term" value="F:ubiquitin-protein transferase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019516">
        <property type="entry name" value="Glomulin/ALF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013877">
        <property type="entry name" value="YAP-bd/ALF4/Glomulin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15430">
        <property type="entry name" value="GLOMULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15430:SF1">
        <property type="entry name" value="GLOMULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08568">
        <property type="entry name" value="Kinetochor_Ybp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="579" mass="65303" checksum="23A30C88F18530F1" modified="2018-12-05" version="1">MALEQLSDVVQRCQALPDDSYSSEDHDVFTVAGRSCIEEGHSPQVLSIVLDEKNQSVVRSMGWNLLPPLVQVLLRKDDKHLPQCLAIFNHLLETCGPKELLIGLLELLEHDDADRIAESFHLLLNPLQKVLLRLGGRKASSLGMTLSSVLDQLAKLPVPDTKEQEEDDVFSLGRCCADLARFVGPFAQEARLNQSTKDELRTELLKFCMKTLSHPLLDVQIWDPDTLPLSPLRTFAAEILDILGAVGESLPGLVFHPLLKKKEVPGFLEEEVRYPKNSLACLAHLVFVHHLAADTFPSVFSPVFCLRCNMEHISILLSRLVRVEDGSLPADLLAIKTFLTVPQNLVKVMTICPKHDLRTKGLKVFQLSIDKFNTEAKYRFFQYMLRTSSHSGVEGYIIKNIKNQIDAALQPGNGNIWFEGVHLLPLLRKVFSLPDGPETDLLQYLDRFMESLNLLRYLVIRDKVTENQTGVWTELYKIEDTFMKPLRVGVNMSRAHYEQELQDTRKKKKKNTKGHKDSVLSVSVGGEKLPKMTSESQIQALHSALHTFDMIESVLVRIEELVEVKDSLETAGEPQLLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPZ6</accession>
    <name>A0A3B5LPZ6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NPC intracellular cholesterol transporter 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Epididymal secretory protein E1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NPC2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPZ6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012840"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012995.1">
        <property type="protein sequence ID" value="ENSXCOP00000012840.1"/>
        <property type="gene ID" value="ENSXCOG00000009691.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006223"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033344">
        <property type="term" value="P:cholesterol efflux"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032367">
        <property type="term" value="P:intracellular cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00916">
        <property type="entry name" value="Npc2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.770:FF:000001">
        <property type="entry name" value="NPC intracellular cholesterol transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003172">
        <property type="entry name" value="ML_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033916">
        <property type="entry name" value="ML_Npc2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039670">
        <property type="entry name" value="NPC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306">
        <property type="entry name" value="NIEMANN PICK TYPE C2 PROTEIN NPC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11306:SF68">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02221">
        <property type="entry name" value="E1_DerP2_DerF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00737">
        <property type="entry name" value="ML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017393376" description="NPC intracellular cholesterol transporter 2" evidence="8">
        <location>
            <begin position="29"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="MD-2-related lipid-recognition" evidence="9">
        <location>
            <begin position="33"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="158" mass="17362" checksum="03B6CC91E3C3DBF4" modified="2018-12-05" version="1">MFLCFRIKNMDVRTGLIVLFCLMGLTWAQPVKYIDCGSTTGKVIGVDINPCPNQPCQLHKGNSYTVNVTFTSNVDSDKSTAVVHGVVGGIPIPFSIPVPDGCRSGIQCPIKTGNTYNYQASLPVKTEYPSIKCVVEWELRDNGNEDLFCIKFPVQIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQ79</accession>
    <name>A0A3B5LQ79_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Lysine-specific demethylase PHF2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Lysine-specific demethylase phf2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">PHD finger protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Lysine demethylase that demethylates both histones and non-histone proteins. Mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2). Recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQ79"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013197"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013358.1">
        <property type="protein sequence ID" value="ENSXCOP00000013197.1"/>
        <property type="gene ID" value="ENSXCOG00000009989.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158148"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140096">
        <property type="term" value="F:catalytic activity, acting on a protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15554">
        <property type="entry name" value="PHD_PHF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1360:FF:000001">
        <property type="entry name" value="Histone lysine demethylase PHF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000193">
        <property type="entry name" value="lysine-specific demethylase PHF2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000011">
        <property type="entry name" value="PHD finger protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041070">
        <property type="entry name" value="JHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050690">
        <property type="entry name" value="JHDM1_Histone_Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23123">
        <property type="entry name" value="PHD/F-BOX CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17811">
        <property type="entry name" value="JHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0010">Activator</keyword>
    <keyword evidence="18" id="KW-0137">Centromere</keyword>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0804">Transcription</keyword>
    <keyword evidence="14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 23" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="25">
        <location>
            <begin position="5"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="26">
        <location>
            <begin position="197"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="452"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="559"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="702"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="754"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="562"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="706"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="793"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="24">
        <location>
            <begin position="851"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="862"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="872"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="915"/>
            <end position="925"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00056118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00068607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00069081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00081977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="953" mass="107683" checksum="1D041D6924524E18" modified="2018-12-05" version="1">MATVPVYCICRLPYDVTQFMIECDACKDWFHGSCVGVDEDDAPDIDIYHCPNCEKTHGKSTLKKKKNWSKHDTGQSTDIRAVQNGSQVFIKELRSRTFPSADDIVVKLSSSQLTLDYLEENGFNEPILVQKKDGLGMSMPPPTFYISDVEKYVGPDVPVDVVDVTKQTDSQMKLKEFVDYYYSTNRKKVLNVINLEFSDTRMSSIVESPQIVRRLSWVENYWPDDALLGKPKVTKYCLICVKDSYTDFHIECGGASVWYHVLKGEKIFFLIKPTSANLSLYERWRSSSNHGEMFFADQVDKCYKCTLKQGQTLFVPSGWINAVLTPVDCLAFSGHFVHNLSVEMQMRFAYEIEKRLKVKTLTPFPNFETACWYVGRHLLERFKGLHKANKQPAPYLIHGAKIINGAFRAWTKKQALLEHEDELPENMKPSQLIKDLAKEIRLSENATKAIKSEPSIKAPVEEPAFTHSEPDEPVSPATIPSPSREKPKKKAPKSQKPPKAPKAPKPPKVKDGEKKKVKKIKELSPPPKPSSFAALESHAKEILSQMDQQKKPKVVKNVLSVSEKETSKQNNMDKFEIREQNKNKTEAKWKYKNSKPDSLLKMEEECKFDRIPLSGNKDRFSFTMSHRKLASSKMLKPQTNSSIFGSLQNLKEDKTKPVRDEYEYVSDEGELKIDEFPIRRKKNTVKRDLSCECFQIKRVNPFSRPKSVDSSDEETLHIDTEAKPEVKSRNSKVKKKSGSAAGILDLLQASKQVVYPSLESEEDNPVFKSRSKKRKSSDDTPYSPTARVGPSVPRHERPAREGARVASIETGLAAAAAKLSHQVGTPERRTRRTRGKGNRVSCVLQEEQQRTKKKKKSGKKKSAAEPPKHSQDSSSPDHNADSQEGSLTDHEFTTGTAKSPGGPQPMAPGIFLSQRRPSVSSPNNTKRLKKGMATAKQRLGKILKIHRNGKLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQ92</accession>
    <name>A0A3B5LQ92_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQ92"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012945"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013105.1">
        <property type="protein sequence ID" value="ENSXCOP00000012945.1"/>
        <property type="gene ID" value="ENSXCOG00000009796.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163009"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000002">
        <property type="entry name" value="Transmembrane protease serine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017315012" description="Peptidase S1 domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="4">
        <location>
            <begin position="25"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="267" mass="29753" checksum="E05409F18C5F6421" modified="2018-12-05" version="1">MAVGKLLALLVLLQSLMGAGVRSSIIGGGDAPKGRWPGLVYLDIKTDSGERKWHCSGSILNQKWIMTAGLFSNNRPQLYTSMWIGTYELDKPSERYMEVNLVVRNPEFRVTGNGFINDIALIKLKEPLEFSKNVAPVNLPKDDDIFDSSSECWIAGWWEKINVWGEKSNTLQEMEVRIVGQTRCEATFPDLSDNMLCAGSPAGGKYCCNHPPGDHGGPLMCRTASGFVQVGIMSFHSPDDCHTPDRFGIYTQVFSYMDFIMTYIMFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQM2</accession>
    <name>A0A3B5LQM2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">V-type proton ATPase subunit C</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6 7">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex. The V1 complex consists of three catalytic AB heterodimers that form a heterohexamer, three peripheral stalks each consisting of EG heterodimers, one central rotor including subunits D and F, and the regulatory subunits C and H. The proton translocation complex V0 consists of the proton transport subunit a, a ring of proteolipid subunits c9c'', rotary subunit d, subunits e and f, and two accessory subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the V-ATPase C subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQM2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013085"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013245.1">
        <property type="protein sequence ID" value="ENSXCOP00000013085.1"/>
        <property type="gene ID" value="ENSXCOG00000009914.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004263"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14785">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1180:FF:000003">
        <property type="entry name" value="V-type proton ATPase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1180">
        <property type="entry name" value="Vacuolar atp synthase subunit c, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015095">
        <property type="entry name" value="AlkB_hom8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004907">
        <property type="entry name" value="ATPase_V1-cplx_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036132">
        <property type="entry name" value="Vac_ATP_synth_c_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10137">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10137:SF5">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09004">
        <property type="entry name" value="ALKBH8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03223">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118203">
        <property type="entry name" value="Vacuolar ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5 7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Alkylated DNA repair protein AlkB homologue 8 N-terminal" evidence="8">
        <location>
            <begin position="3"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="277" mass="32498" checksum="F8CAE7222B9FD1FE" modified="2018-12-05" version="1">LTWNMNTLELIKKAQQRLNFLRVLRRNNIKEKLLVSFYKCSIESILTYCICVWYNSSTTAQRKALQRVVNTAQKIIGCPLPSLEDLHSDRCLRKAQHITKDTSHPGPSLQLFEDNDSGLFSVTLFRKAVDDFRHKARENKFTVRDFQYNEEEMKADKEEMTRLSTDKKKQFGPLVRWLKVNFSEAFIAWIHIKALRVFVESVLRYGLPVNFQAMLLQPNKKNMKKLREVLNDLYKHLDSSAAVIDASMDIPGLNLSQQEYYPYVYYKIDCNLLDFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQW8</accession>
    <name>A0A3B5LQW8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Choline transporter-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Choline transporter.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Choline/H+ antiporter.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>choline(out) + n H(+)(in) = choline(in) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:75463"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQW8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013190"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013351.1">
        <property type="protein sequence ID" value="ENSXCOP00000013190.1"/>
        <property type="gene ID" value="ENSXCOG00000009981.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156600"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385:SF42">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="324"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="567"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="612"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="679" mass="76636" checksum="10CBD9EBAB09C9E7" modified="2018-12-05" version="1">MSLSVIYACVLIYRKCTDVVCCIIFLIVILGYIALGTVAWFHGDPRKIISPTDSQGQFCGQTDTSNSNKNILFYFNMLKCASPAVLINLQCPTTQLCVSKCPDRFATLSEVSVNKSWEYYRQFCKPGFVLGSKVGNILQRCFPEFKSKEGLLTVANQTTFRDPRNNTWTVNDLRDAAMGIASLLNAKEIGVKILEDYANSWVWILIGLGMTMVFSLSFILLMRYIACVILWLMICGIIIAAGYGGWRCYKEYVLLLEKPDTNVAISDIGFQTDLSIYLEMSQTWLVLMILLILIVVVIVLMLIFLRKNLSIAFALMKEGSRAIFYIICTLFYPIVTFFLLAICIAYSAVTAVFLASSGNAIYKVTSPDDKCIHRNLTCSPKTFSQTNIAKECPGAQCMFAFYGGETVYHNYIILLHVCNLFVFLWLVNFVLALGKCTLAGAFASYYWAKRKPKDIPTCPVYASFSRAMRYHTGSLAFGSLLLAMVQMIRIVLENLHLKLKRSQSCMSRFLLHCLKCCFWCLERFIKFLNTNAYIMIAIYGHSFCKSSRDAFDLLMRNILRVVVLDNLITFLLFIGKLVISGGIGVLAFLFFSKRIPIVQDMVPQLNFNWVPLLTLIIGSFMISSGFFNVYAMCVDTLFLCFCEDLERNDGSSSRPYYVSPGLHKILRKGEERAKSSASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LR32</accession>
    <name>A0A3B5LR32_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Kelch-like family member 29</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LR32"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010244"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010367.1">
        <property type="protein sequence ID" value="ENSXCOP00000010244.1"/>
        <property type="gene ID" value="ENSXCOG00000007776.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158824"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd18258">
        <property type="entry name" value="BTB_POZ_KLHL29_KBTBD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF10">
        <property type="entry name" value="KELCH-LIKE PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="782"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="326"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="823" mass="89428" checksum="5BBC7D136BC2B254" modified="2018-12-05" version="1">MSRHHHRFERDYRGPWDRRDIRPSGLHNGGANHSCASAVVNGNNRPGLLPLPVIPSLLPTPVTVAVTTSSCDLVVKRGITAAGSVAPVSGLSPGDNQTPGLLLAPGVRWGQTPINQLTPWDTDEPPAKQHRDGEPTGPAWAAPVAAVSQQSLLPHSYGLPQPPSFSHSVTAQSPIIGVTPSIQTQVAPQHPLMTTHFQLTPQSTQQGPSLVLSMPSQPPYPAAQPTVTPTALTAQANPVVHPNPHSLMGNGHLTSHSSLLQPPPLPLTNGQAAVHAQPTPADNQDAPNGLQMLRTVGSGKYEFSDPGHPKEMLKELNQQRRTKEFTDLKIIVEGKEFEVHQNVLASCSLYFKDLVKRSSGETKSGETLELTMSNIGADVLELLLEFVYTGSLVIDSANAKTLLEAANKFQFNTFCKVCVSFLEKQLTAANCLGVLAMAEAMSCTELHNMAKAFALQNFPEVAGQEEILSVSKEDLVTYLSNDSLNTKAEELVYETVIKWIKQDPSSRVQSLLIRSLPGLLPFTHPPACCLQTHSLFLSGRSACSLEPAGAANREDLRTHVAACVAEVIVLLGGRQAIGMNQRSLTAVTCFNPQNSKWYPLASLPFYDREFFSVISAGDNIYLSGGTESGVMVADVWCYMSLLDNWNLVSRMTVARCRHNSLVYDGKLYTIGGLGVSGNLDHVERYDTITNTWETVSPLPKPVHSAAATVCGGKIYVFGGVNEAGRSAGVLQSYIPQTNAWSFIESPMIGKKSLSSLIGFICSYWNSCYEFGCNKTDPVYTCLAIYFYLFPLVGCFLLDFEFIVVEVPRLFDCKICFIHKLMSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRH8</accession>
    <name>A0A3B5LRH8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">X globin</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="5">Monomer. Homodimers and homotetramers. Mainly monomeric but also detected as part of homodimers and homotetramers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the globin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027855991.1">
        <property type="nucleotide sequence ID" value="XM_028000190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRH8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014663"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014849.1">
        <property type="protein sequence ID" value="ENSXCOP00000014663.1"/>
        <property type="gene ID" value="ENSXCOG00000011133.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114134006"/>
    <dbReference type="KEGG" id="xco:114134006"/>
    <dbReference type="CTD" id="497122"/>
    <dbReference type="GeneTree" id="ENSGT00730000111686"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098809">
        <property type="term" value="F:nitrite reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd12137">
        <property type="entry name" value="GbX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050532">
        <property type="entry name" value="Globin-like_OT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46458">
        <property type="entry name" value="BLR2807 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46458:SF2">
        <property type="entry name" value="X GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00188">
        <property type="entry name" value="PLANTGLOBIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Globin" evidence="8">
        <location>
            <begin position="30"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="202" mass="23310" checksum="C3A6B3B67598A779" modified="2018-12-05" version="1">MGCAISGLATKTEFSQRDREEAAAAAAAAHLSEEQVQMIKDSWKVIRDDIAKVGIIMFVRLFETHPECKDVFFLFRDVQDLERLRTNRELRAHGLRVMSFIEKSVARLDQPERLEALAVELGKSHYHYNAPPKYYNYVGTEFICAVQPILKERWTAELEEAWKTMFQYVASLMRQGYQDESAQQRHLAASPKDRPDKKNTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LS96</accession>
    <name>A0A3B5LS96_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Neurabin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Neurabin-I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Neural tissue-specific F-actin-binding protein I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Protein phosphatase 1 regulatory subunit 9A</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LS96"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010813.1">
        <property type="protein sequence ID" value="ENSXCOP00000010689.1"/>
        <property type="gene ID" value="ENSXCOG00000008073.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164127"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06790">
        <property type="entry name" value="PDZ_neurabin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09512">
        <property type="entry name" value="SAM_Neurabin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000010">
        <property type="entry name" value="Neurabin-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000008">
        <property type="entry name" value="Neurabin-1 isoform 1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040645">
        <property type="entry name" value="Neurabin-1/2_PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043446">
        <property type="entry name" value="Neurabin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16154">
        <property type="entry name" value="NEURABIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16154:SF26">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 9 LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17817">
        <property type="entry name" value="PDZ_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="PDZ" evidence="19">
        <location>
            <begin position="613"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="18">
        <location>
            <begin position="1100"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="393"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1159"/>
            <end position="1196"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="846"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="394"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1160"/>
            <end position="1185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00076637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1207" mass="135311" checksum="CDB78A43E62A26B8" modified="2018-12-05" version="1">MMRTDSRGRSASPHRITYKSDFHAIKCTFDTGISLQGGPQTAAACQGSAVPCDDLSTSSVTGSRGRIQGTRGTKIRENIFLQMDSQQSRQDGAPVLGSGSIPLLSASHPSLQLNTSPFPESRHSVMSFSPSFLSSLASISSTEKLLQDKPSRSEKKVDIDRVALAQKFSVTRRLFESKMLEVGRVGGGGEQVLKPQIIRGSKGAAVLKGEVEKLQEASEKIKEKDVSDKDLSINHNINIKAPQAESLMRHYESSVLNGPGEVATSQSKEETGVGLAPEETLRAELVNVKNDSSESDENEEERVQKDKYCTKGEIEMHLNKADEEDQKASVGDVFEEDVFVETPESLHTFTEGRDNLITSSEQHTELSKDVQWKTTGNGGERRGDMYQQVNKQWEGGREEEQHRHFTAQTKKNQTEESEDEEKTTDGGFRESAAGLEVLIQEMFSDKEGGGKVEGGEEGFRHSQRTEIKEGTTSENIVTEKMMHVSCSDGEGGKDDQTEAVCGIETRAFWYDKDSQSHPELSASLQEEQESCMKGGNQLAAEYEEIPGVPELDCQNEDSPEAAKRKVRFSTAPIKVYSTYSNADYDRHNEDIDPVSASAEFELEKRVERMDVFPVDIEKGVDGLGISIIGMGVGADQGLEKLGIFVKTITEGGATHKDGRIQVNDQIVEVDGVSLVGVSQLFAATVLKNTSGLVKFLIGREKEGVESEVARLINESLEMDKTKQVSLGVSERDSIFEEEEVEEDEDEEEDASVLSSLDNYQLCLKYQQTCEGKIKMYPSYSVMAVVSRMMLLVVNMVIRLALIVSYWQEAQTLCRVVSQRLADTQGQTESLEVKYGKAKRLVRDYQKEEMDKKLADMTNDMEEREKQHRETIERLQTQVRGRYYKSVWIHFLHVLLCVLKDQSKILYAKNNKRNLFFSDWYLPVPNSGRLDSSAHIARAQLAQKSKRNPPSRDKLRESFKKQCYSFCICINSLYYSCFLRGFFSILDKLCQKDTGKPCFLVTSEACNNFTVSSGSCGDLIDEPLEVSPSGLFNSVPSCLHLPWFGDRGKEKEEGEQNRQRLRSVSISFFFLTSLISHQTLDDYPIPNNNNNRWQCQPLSEWNNQQVCLWLISMSMNRYTKEFTAREVDGTQLLHMDSQKLKALGVSNQNDRSVLKKKLKEMRREEKDQRKEGKKLKEGEGGERARMLSDTSVNDVGGCGKTVRTESLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSE9</accession>
    <name>A0A3B5LSE9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSE9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012316"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012465.1">
        <property type="protein sequence ID" value="ENSXCOP00000012316.1"/>
        <property type="gene ID" value="ENSXCOG00000009309.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158721"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17979">
        <property type="entry name" value="DEXHc_DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000013">
        <property type="entry name" value="ATP-dependent RNA helicase DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000540">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000725">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056382">
        <property type="entry name" value="DHX34_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF221">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24485">
        <property type="entry name" value="zf-C2H2_DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="166"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="362"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="692"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="701"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="748"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1133" mass="128707" checksum="7E409AC823D9A677" modified="2018-12-05" version="1">MDRDRSRDSWDWGSPQNRAQMDEIFFREQDYIQAGCTEHKKFWAFFDRFQRFKAKREMSGSGIRDDVKEKKRSGSEKVDLGLPKEYDARYRINVSVCTGDIEKRVRKSDPRSRQRSSGPGPQEVSDCRLALLHFLDFSQKQSFGKLAKLRREQKNLPIFQYRDRIVELVRRHPVIVVAGDTGCGKSTQVPQYLLSAGFSHIACTQPRRIACISLAKRVSFESLNQYGSKVGYQIRFETSRTAATKLLFLTEGLLLRQIQQDKILDQYQVVIVDEVHERHLHCDFLLGVLRSLLAERPDLRLILMSATINIKLFSEYFRSAPVLQVPGRLFPIQVIYQPIPPEEQPSRSEKLDPRPYLRILQGIDQRYPPEERGDLLLFLSGVAEISNIQEACQVYATHTRRWIVLPLHSTLSLAQQDKVFDVSPPGVRKCIISTNIAETSVTIDGVRFVVDSGKVKEMSFDPKAKMQRLQEFWISRASSEQRKGRAGRTGPGVCYRLYSESDYNAFAPYPVPEIHRVALDSLVLQMKSMDLGDPLSFVFIDPPPTASIQTAVTYLKEQGALDSRGELTSIGKLLAQLPVDVVIGKMLVLGSLFNLVEPVLTIAAALSVQSPFLRSSQQNPDCKTSCQPLHSNQGDPFTLLNTFNAWVEVKGERGGGSRKWCRRRGLEEQRLYEMVNLRRQFKNLLRSHGLLESEDTAPSSGDREQRRERFTERKKLHQLKRDHEQQEGTKRKVLRLEEGGEFSSGSDSEDRGRGRKSKEGQGQEVDIQEVKFKLRHNVSELQEAVGASQDLSSRHQALLKLLLCRGLYPQLALPDEHNTTRKDSDQVFHTRNKQGVVMHPTSVFASDPEVLHVPEDEPRETGPDRRDSSRHQLLAFVTLLETNKPYLSNCVRVPALQALLLVANSVDTNADCTRLVVDGWLELEVREPDEALRVLSRALSLRSEWERLLKAQLGQSATSGQGVSRRDMEKLSDGLVRFLLYTEVSYSLQRLTALQTQNLYVGPQADPDSVYSELPDLSLLFPGAEAKPDPIKGGLRVTSFFIYNCLADSKDLYSECLRTFWSCPNCDLYMPLTPLERMHHEASCRPAGERQESGSSGKSSLTRVYHCDVCDEDLTLTSTEILKHKRQHTFSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LT72</accession>
    <name>A0A3B5LT72_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydroxysteroid (11-beta) dehydrogenase 1-like a</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LT72"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012576"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012727.1">
        <property type="protein sequence ID" value="ENSXCOP00000012576.1"/>
        <property type="gene ID" value="ENSXCOG00000009506.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162487"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051253">
        <property type="entry name" value="11-beta-HSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44279">
        <property type="entry name" value="HYDROXYSTEROID (11-BETA) DEHYDROGENASE 1-LIKE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44279:SF2">
        <property type="entry name" value="HYDROXYSTEROID (11-BETA) DEHYDROGENASE 1-LIKE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="302" mass="33483" checksum="39E118233AC377A0" modified="2018-12-05" version="1">SELRTKKKDIINGFHCICFFECVDFVLESLKGARVLVTGASSGIGEQMAYHYARFGAQIVITARREKVLQQVAEKCLSLGAQKALHIAADMANESDPERVVDFALEKLGGLDYLVLNHIGPTTFGMWEGDVEHTRWLMKVNFFSYIQMAWRALPSLEQSKGSLVVVSSVLGKMTNPFTLPYSSTKFALSGFFGSLYHELAMKKSNVSISVCTLGLIDTDSAMDKVRGITKVPAYPATEAALNIIIAGATRQLDLYYPWFTQVITLIKDWSPSGGVTLDRSPVCRRATQRHTTMHTHSHLGRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTV4</accession>
    <name>A0A3B5LTV4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Peripheral myelin protein 22a</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the PMP-22/EMP/MP20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027898631.1">
        <property type="nucleotide sequence ID" value="XM_028042830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTV4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014457.1">
        <property type="protein sequence ID" value="ENSXCOP00000014280.1"/>
        <property type="gene ID" value="ENSXCOG00000010823.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114160293"/>
    <dbReference type="KEGG" id="xco:114160293"/>
    <dbReference type="GeneTree" id="ENSGT00950000182696"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050579">
        <property type="entry name" value="PMP-22/EMP/MP20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004032">
        <property type="entry name" value="PMP22_EMP_MP20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671">
        <property type="entry name" value="EPITHELIAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671:SF7">
        <property type="entry name" value="PERIPHERAL MYELIN PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01453">
        <property type="entry name" value="EPMEMFAMILY"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01221">
        <property type="entry name" value="PMP22_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017367757" evidence="7">
        <location>
            <begin position="27"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="154" mass="17483" checksum="A1D6E184D10BC2BA" modified="2018-12-05" version="1">MLCILLGVLALHLLVFILLIVSTASSQWTVEARGHTDLWYNCHSDTQGYHCSQASNEDWIQAVQALMILAVVFCFVSLLAFAYQLFRLFKGGRFFFTSIFQILASVFVMCAAIIYTVMRPDSDKDYGYSYVLAWVAFPLSLISGLIYLILRKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUD2</accession>
    <name>A0A3B5LUD2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUD2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013081.1">
        <property type="protein sequence ID" value="ENSXCOP00000012921.1"/>
        <property type="gene ID" value="ENSXCOG00000009781.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286958"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001480">
        <property type="entry name" value="Si:cabz01071911.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001506">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000681">
        <property type="entry name" value="zinc finger protein 205 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF142">
        <property type="entry name" value="ZINC FINGER PROTEIN 384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="117"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="201"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="248" mass="28287" checksum="C1BF684F157B8EF9" modified="2018-12-05" version="1">MDENRPVARTAVKDSSEDDEEKPQVHLPELHHNQTEESEDFVGPEPDQHLESDVEDKTSDSSSDSEAQYGLGSGTNNTDSVCERDKKLHRCSECSKTFSRRRYLLEHKRIHTGEKPYSCSICDKTFTWKQSLTLHMRTHSEEKPFSCFVCTKAFTSKANLKEHTKVHTQHRPCRCPICNAAFKRKNNLIEHIRIHTGEKPYSCPVCSKAFTSKGNLQKHTKIHAEERPYICSVGLDGSDSKLISHEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LV78</accession>
    <name>A0A3B5LV78_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Divergent protein kinase domain 2Ab</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DIPK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LV78"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015047"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015236.1">
        <property type="protein sequence ID" value="ENSXCOP00000015047.1"/>
        <property type="gene ID" value="ENSXCOG00000011404.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00520000055625"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020519">
        <property type="entry name" value="DIPK2A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022049">
        <property type="entry name" value="FAM69_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32073:SF6">
        <property type="entry name" value="DIVERGENT PROTEIN KINASE DOMAIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32073">
        <property type="entry name" value="GH11358P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12260">
        <property type="entry name" value="PIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="FAM69 protein-kinase" evidence="5">
        <location>
            <begin position="206"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="431" mass="49806" checksum="11AF02D13F08F076" modified="2018-12-05" version="1">MLRFLPLKLGRLYRCLKLLLVLGLSVILLMNTHNLFASFQKNELTDRRFINLNKCPACFGTSWCRKFMNGQVSFETWGRLRFLDVFNVKNVYFAQYGEPREGTRRVVLKRLGSNQELAEIDQKICKRATGRPRCDLIQAMYKTEFARINGDVRLLTPGVVEGWSDLVHCPSQRLLDRVVRRYAETKDSGSFLLKNLKDTERMQLLITLAFNPEPLVLQSFPSDEGWPFAKYLGACGRMVAVNYVGEELWSFYNAPWEKRVDLARQLMDIAEQLTNNDFDFALYLLDVSFDNFAVGPRDGKVIIVDAENVLVADKRQIKQDKPENYDVWYESRFEECDREACLSFSKEALCSRVTVDHNYYAVCQNLLSQYATWRGTTGGLLHDPPAHIAKDGQLEALLDECTKPKKRYGRFQAAKELREYLTRLSAAQYDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LX01</accession>
    <name>A0A3B5LX01_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zgc:175214</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LX01"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016453"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016660.1">
        <property type="protein sequence ID" value="ENSXCOP00000016453.1"/>
        <property type="gene ID" value="ENSXCOG00000012416.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166025"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763:SF190">
        <property type="entry name" value="RING FINGER PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="91"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="155" mass="17830" checksum="5ED2DB66AF3FF3EA" modified="2018-12-05" version="1">MQPFQWCNGCLCGLSFHRSEHYCSMTSDIYHLPLNVYVIVLGIGLFVFMLSLIFCCYLFRLKQQGTREQFSYNEVRRTHTCHCGGLCNQTCAVCLEEFRTRDELGVCPCSHAFHKKCLLKWLEIRSVCPMCNKPILRLHSDAPQGAEGPMDPEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXE3</accession>
    <name>A0A3B5LXE3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Late endosomal/lysosomal adaptor, MAPK and MTOR activator 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator plays a dual role for the small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD): it (1) acts as a guanine nucleotide exchange factor (GEF), activating the small GTPases Rag and (2) mediates recruitment of Rag GTPases to the lysosome membrane. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LAMTOR3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXE3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012489"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012640.1">
        <property type="protein sequence ID" value="ENSXCOP00000012489.1"/>
        <property type="gene ID" value="ENSXCOG00000009440.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013159"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071986">
        <property type="term" value="C:Ragulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032008">
        <property type="term" value="P:positive regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.30:FF:000003">
        <property type="entry name" value="ragulator complex protein LAMTOR3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015019">
        <property type="entry name" value="LAMTOR3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13378:SF1">
        <property type="entry name" value="RAGULATOR COMPLEX PROTEIN LAMTOR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13378">
        <property type="entry name" value="REGULATOR COMPLEX PROTEIN LAMTOR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08923">
        <property type="entry name" value="MAPKK1_Int"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Roadblock/LC7 domain"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="124" mass="13688" checksum="CE7A487054DD774A" modified="2018-12-05" version="1">LLQDLKRYLYKQLQSVEGLHAVVVTDRDGVPVIKVANDSAPVHALRPGFLSTFALATDQGSKLGLSKNKSIICYYNSYQIVQFNRLPLVISFIASSNANTGLIMSLEKELAPLIENLRQVVEVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXJ4</accession>
    <name>A0A3B5LXJ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXJ4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015867"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016064.1">
        <property type="protein sequence ID" value="ENSXCOP00000015867.1"/>
        <property type="gene ID" value="ENSXCOG00000011984.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052074">
        <property type="entry name" value="NonRcpt_TyrProt_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46900:SF4">
        <property type="entry name" value="FERM AND PDZ DOMAIN CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46900">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="1">
        <location>
            <begin position="15"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="161" mass="17095" checksum="E8194A0A8AE0D3D3" modified="2018-12-05" version="1">SDGSVTYCLMGNGLTIMFKKPEGGGLGFALVGGANGTMLRVREICSGGVAEQDGRLKVGDILLEVNGVIVSGLSHSKVVDILRKAEGTVQLTICRDILPLTYSESPTPPNMSAHTEAILAEQPAHMSNSDVCSAPESLQDREAERLVGMISSFCLSFYQFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LY84</accession>
    <name>A0A3B5LY84_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000015880"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016077.1">
        <property type="protein sequence ID" value="ENSXCOP00000015880.1"/>
        <property type="gene ID" value="ENSXCOG00000011996.1"/>
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    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025:SF27">
        <property type="entry name" value="SI:CH211-193K19.2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31025">
        <property type="entry name" value="SI:CH211-196P9.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="151" mass="16971" checksum="B7666E839D2DA308" modified="2018-12-05" version="1">IAETFLGIFVIPPEGAEAADAHLDVGIVLEGVEVMNSLGSVLFAIVMLLGLIYALNLSYPEELKHTFEVLQKIIMKMDGKNLSKKAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGITNFLLIMLRWENWKLKIQNYEKLLCIRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYU3</accession>
    <name>A0A3B5LYU3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ARID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000014516"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014701.1">
        <property type="protein sequence ID" value="ENSXCOP00000014516.1"/>
        <property type="gene ID" value="ENSXCOG00000011009.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155634"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035060">
        <property type="term" value="C:brahma complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071565">
        <property type="term" value="C:nBAF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
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        <property type="entry name" value="ARID_dom"/>
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        <property type="entry name" value="ARID_dom_sf"/>
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        <property type="entry name" value="ARM-like"/>
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        <property type="entry name" value="BAF250/Osa"/>
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        <property type="entry name" value="BAF250_C"/>
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        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BRG-1 ASSOCIATED FACTOR 250 BAF250"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ARID" evidence="5">
        <location>
            <begin position="910"/>
            <end position="1001"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="96"/>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Ensembl" id="ENSXCOP00000014516.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    <sequence length="2041" mass="219830" checksum="FA8E2F8D4A6905A1" modified="2018-12-05" version="1">MAAQVASAPTRTNNKNKKLSGALAPELNSGKSGGGGGGAGTAQRSGQMLGAGDGTLNNVDHHRRGELGSMVHSTSAAHKAPDGHDKTGINISASNSNTSPMEAALIANHKLKCAASGDAPQPQQQQPPPPQFGQFAPHQQRQSNNSNNGQPPRGDRSVDHQQHGGKENLLVGQEETQQQHGPGKGEGDLTCKPTERMMGSRFEHSNLGPPTNNSEFNNSYYTSRPCYDQHAGQQQQSSGMGVTQDSNEGGYHNSQYGQYPAYRAGYGGGAYGMIGSSGCRQPGNMMMGSNSSATHSKSPASGGFQRYPGQTQQQQGPSGATPTLNQLLTSPSPMMRGYGGAYQDYGGPTPQQQAGMALGKDVGPQYGASTGHWGGQQRNHPLSMNAGSGGQGLGRTQVPSMDFMAMKRSQMYGMTNNPYSTPQQPGAGNYPSSQPYASPPSHRYPMSMQGRSQMSMSGMQYPQQQVAPYGQQSMGGFSREQQGGQQGTPPYFSPPQQTPTGLSQSSYLQPRPPPQQVLSLTYRTLSLDKGACCSLLPKQVSPSLLAFQDLSGSIDDLPTGTEAGLGSAVGAGGSSNGSSGGSSQGEPGGAGNQGQSPFSPHASPHLPGQRSGPSPSPVGSPAGSTQSQPSRSGSRPISPASGPPANTTAPGRVSLSTHLPITRSPMAQERGFMSNMQRNQSGSQFAPPQSGPPMSPHPSHGGSMYPSMGTYSQSGPAGPYGPQGPQYGHQGNYPRPSNYIGSAGSNYSGPGLSNSLGMNATSPMHGQGPGGRSHAPGSQNRTYPIMGPTSPSMPQPAGPGMGPPSNRKPQDGAVANSVHNRQGNYQAPGINQVSSGSYSQPTSNNSSTTGNGQGPGYGMPPAETIHKISKVSSSETHIYTLVCVPQQKVNPNSSSIGGERISRLYELGSEPERRSWVERYLTFMEERGTPVSQLPVVGKKPLDLWKLYVAVREIGGLAMVNKNKKWRELSSTLNVGTSSSSASTLKKQYIQYLFAYECKMERGEEPPIDSNSGGSLTEGDSRKQVKIQPPSPANSGGSLQGPQTPQSSGSSSTAEAAGDLKPPTPATTPLGQVTPLPPNRSSVSVQDPFSEVSDPAFQKRTSSLPPSAPYQQALGMPDMMMRMQYDAKDPFAGMRKSKQSAHCVTQFTKNGGMQDLYSRPPSGLSMSQRSQYPYGPVTYLHCVLLLLFIQGYKRQLDGMYGPPPKRHESDMYAMQYTNQQPDVYNHYSGGYSGPDHRPIQGQFPYPYHRDRMAPSGQSQHSMMGGGPASNHAPEGPNMWPSRTDLSYSYHSRQGPPSQMQPYGSICRDDIEGRSAHDHWLRQPPYISSSGGMAPMSSRHSSYSNSPTMANHLPRAPSPGAFQRSMESRMSPSKAPFMSPMKMPKPGMAMMGSQGSGPNGQFPPNLRRDLNYPPGSVEATMPALKPRRKLTSKDSGTPEAWRVMMSLKSGLLAESTWALDTINILLYDDSTVASFNLSQLPGFLELIVEYFRRCLIEIFGILEEFEVRTMVPKNDLHSTEIQERQTGVIKEEMSPVTEEQAEVPEKEQDPDVTKKKKNSNKGQSEKGLKEPSAETESKPKQASKYDKLPIKIVHKDDPIEDITERLGYVTEFDSGLIHWQAGGGDSTAHIQTHFELRSDLPARGGTKTLNENKPENNTEGEDSKKLKHITATIDDVLFARVDALSYAHPARSLPSYPFRVHPDGEDDHITLLEDEPRCLDEAPLCTTSSWQDSLSKRCLCVSNIVRSLSFIPGNDSEMSHHPALVLLLGRLLLLHHVHPERNRSAPTYQKDEQQERGLSSSKEEWWWECLSLLRENAMVTLSNIAGHLDLSTYSDTICLPILDGLLHWMVCPSAEAQDPFLSTSPHSQLAPQRLVLECLCKLSIQDGNVDLLLATPPFSRQEKFFAVLVRYVGQRKAQVYREMAVAILSHLAQGDPTAARAIALQKASVGNLVTFLEDGVSMAQYQQNPHNLLHMGHPPMDPPSVNMMCRAAKGLLAMAKVEENKKEFVLYESRLLDISISSVLNTGVVAIVCQVLFHLGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZS9</accession>
    <name>A0A3B5LZS9_9TELE</name>
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        <submittedName>
            <fullName evidence="8">Zgc:112294</fullName>
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    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="TRANSMEMBRANE PROTEIN 17B"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="47"/>
            <end position="65"/>
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            <begin position="144"/>
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            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013254.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="210" mass="24284" checksum="DB111D84A2F6B511" modified="2018-12-05" version="1">MPVFYSPVPDNLQMGLAYMGGSVFTNNRTADSDFMVNELVSHLPLQMLLYFNLFYFPCWWFSAVFMLEVKYYYLPGYYQALLITGLVLVTVIEVIRLYLGYIGNLKEKVPALAAFWLLSFTFQLPVLLFFITDEGVIILPLERAVHFIYLIFLLAQILASFLALRTMTRKLTLLFHLRQFGKVESLRHAEMSPIYGLPYNRSVLPVSPTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2Z2</accession>
    <name>A0A3B5M2Z2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP-2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M2Z2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018483"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018715.1">
        <property type="protein sequence ID" value="ENSXCOP00000018483.1"/>
        <property type="gene ID" value="ENSXCOG00000013904.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182848"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014032">
        <property type="term" value="P:neural crest cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014036">
        <property type="term" value="P:neural crest cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071698">
        <property type="term" value="P:olfactory placode development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043049">
        <property type="term" value="P:otic placode formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004979">
        <property type="entry name" value="TF_AP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013854">
        <property type="entry name" value="TF_AP2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10812:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-2 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03299">
        <property type="entry name" value="TF_AP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01748">
        <property type="entry name" value="AP2TNSCPFCT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor AP-2 C-terminal" evidence="8">
        <location>
            <begin position="65"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="269"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="269"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="293" mass="32019" checksum="D89A5E32F9F59AC1" modified="2018-12-05" version="1">LHAKKQTFKEGFRAYVHTLEKGICKQSLFFPFSPGPVTLPKGNTLGLPFQKESLLGMVSNPTEVFCSVPGRLSLLSSTSKYKVTVAEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLREKLDKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYVCETEFPAKAIAEYLGRPHVERNEVNSRKNMLLAAKQICKEFTDLLTQDRSPLGNSRPAPILEPGIQGCLTHFSLITHGFGSPAICAAMTSLQNYLNEALKQVDKMYLSSGSETQGSSESGSKSTDKMDKHRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3B3</accession>
    <name>A0A3B5M3B3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Macrophage stimulating 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase S1 family. Plasminogen subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3B3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017850"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018074.1">
        <property type="protein sequence ID" value="ENSXCOP00000017850.1"/>
        <property type="gene ID" value="ENSXCOG00000013438.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159461"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046425">
        <property type="term" value="P:regulation of receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00108">
        <property type="entry name" value="KR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd01099">
        <property type="entry name" value="PAN_AP_HGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000063">
        <property type="entry name" value="Hepatocyte growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.20.10:FF:000002">
        <property type="entry name" value="Hepatocyte growth factor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.20.10:FF:000004">
        <property type="entry name" value="Hepatocyte growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000055">
        <property type="entry name" value="Hepatocyte growth factor-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.20.10:FF:000009">
        <property type="entry name" value="Hepatocyte growth factor-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.20.10">
        <property type="entry name" value="Plasminogen Kringle 4"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024174">
        <property type="entry name" value="HGF/MST1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000001">
        <property type="entry name" value="Kringle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018056">
        <property type="entry name" value="Kringle_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038178">
        <property type="entry name" value="Kringle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050759">
        <property type="entry name" value="Serine_protease_kringle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24261:SF12">
        <property type="entry name" value="HEPATOCYTE GROWTH FACTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24261">
        <property type="entry name" value="PLASMINOGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00051">
        <property type="entry name" value="Kringle"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00024">
        <property type="entry name" value="PAN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001152">
        <property type="entry name" value="HGF_MST1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00018">
        <property type="entry name" value="KRINGLE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00130">
        <property type="entry name" value="KR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00473">
        <property type="entry name" value="PAN_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57414">
        <property type="entry name" value="Hairpin loop containing domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00021">
        <property type="entry name" value="KRINGLE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50070">
        <property type="entry name" value="KRINGLE_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0420">Kringle</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 8" id="KW-0721">Serine protease homolog</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Apple" evidence="12">
        <location>
            <begin position="32"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="10">
        <location>
            <begin position="122"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="10">
        <location>
            <begin position="203"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="10">
        <location>
            <begin position="297"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="10">
        <location>
            <begin position="384"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="11">
        <location>
            <begin position="496"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="204"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="225"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="319"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR001152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="723" mass="82274" checksum="2BDE0853A4A32432" modified="2018-12-05" version="1">MCAGSCPNVADGATDLLLTVSISINTFTSALCLGSLNDYQRSEGRELVPTAWNAARMQMLPGLNLEECASRCSQSQDCRAFNYEIRPIGTCKHLPWVADGSNAEVKRNVNCDLYEKKVYVRKCIVGKGEDYRGKVFTTKSGLTCQQWWSKFPHDHRWTPSPTNGLELNYCRNPDGDRIGPWCYTTDPERRYESCNIPQCKDEVCITCNGEDYRGQVDHTESGKECQRWDQQFPHQHIYQPEKYPDKSLDDNYCRNPDASPVPWCYTTDTETERESCDISKCTEVLVEKRQRSSFTTNCFRGRGEDYRGKVNETASGIPCQRWDVQVPHRHPFIPNIYECKGLEENYCRNPDGSEAPWCFTSVPEMRTVLCLQIKRCADDIEAEDCYQENGKNYRGVVRKTRKGITCQKWNVNTPHQTRINPRTHPDANLTENYCRNPDGDQHGPWCYTTDPKTEFDYCAIKQCAGEKVPLTETVETVEFSECGKRDDRPQPTRLRIVNGIPGNSPWTVSLRDRKGNHFCGGSLVNPRWVISTKQCFSSCYVDLPGYSAMMGTLFRDPQEGEPSMQTIPLTKIVCGPSESQLVMLQLEYPAQFNERVSQICLPPERYIVPEHTKCEIAGWGETRGTGDETVLNVAHVPVISNTECNRYFRGRVRENEMCTNSFQAGVGACEKDYGGPLACQNADCWVLEGVIIPMRRCGHPGQPNIFIRVSVYVDWIKKVMEMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M410</accession>
    <name>A0A3B5M410_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ral guanine nucleotide dissociation stimulator-like 3a</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M450</accession>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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        </subcellularLocation>
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        <text evidence="2">Belongs to the type II (or gamma) interferon family.</text>
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    </dbReference>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="211" mass="24174" checksum="59E1DFBCDC34C213" modified="2018-12-05" version="1">MRMGAVMRKVVCLLAWLSVGQVSGSYLPQDMNRTLQDLLQHYKISKSDIYDGKHVFPKEELKGKMESQMLFMGGVLEAYDKLFGHMLKGLPTPSPLSATSGDKADTSGPSTTEPSAAGDTRFNLIKVLQKIRELKRHSYQEQLKVLHGLQKLKHIEMDNIKVQGKALWELPWIYEKANSLADNVMRRRRRRRRQTRTGLAREPERRAKGQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M526</accession>
    <name>A0A3B5M526_9TELE</name>
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        <submittedName>
            <fullName evidence="9">von Willebrand factor C domain containing 2 like</fullName>
        </submittedName>
    </protein>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Atherinomorphae</taxon>
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            <taxon>Poeciliinae</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Synapse</location>
        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="ENSXCOG00000013907.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="VWC2L_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46252">
        <property type="entry name" value="BRORIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46252:SF2">
        <property type="entry name" value="VON WILLEBRAND FACTOR C DOMAIN-CONTAINING PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="VWC"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017419879" evidence="7">
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            <begin position="23"/>
            <end position="225"/>
        </location>
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        <location>
            <begin position="117"/>
            <end position="175"/>
        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="225" mass="24823" checksum="409E65F72EE1185F" modified="2018-12-05" version="1">MGPLLSAASVLLLFLLLALSVASPATVGNPEDYVADEAERTAADNIVFDDYRGKGCVDDSGFVYKLGERFYPGHSNCPCVCTEDGPVCDQPECPKLHPKCTKVEHNGCCPECKEVKNFCEYRGKTYKILEEFKPSPCEWCRCEPNNEVHCVVSDCAVPECVNPVYEPEQCCPICKHGPNCFAGTTIIPAGKEVKVDDCTICRCHNGDWWKPAQCLRRECLNGQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6Z1</accession>
    <name>A0A3B5M6Z1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6Z1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017336.1">
        <property type="protein sequence ID" value="ENSXCOP00000017116.1"/>
        <property type="gene ID" value="ENSXCOG00000012903.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000267866"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="106" mass="12268" checksum="2DBAB4119D01BCD6" modified="2018-12-05" version="1">ELYVEVVGVHLQFLRVQHAQVFVCGLNVVHVLHSFLQTCHDDLSVSRHFGVSKAAKVPLSPGVDNQTPEETKRSLKRIKPQINTFLGKENRFFFTWRELHSQQHHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M791</accession>
    <name>A0A3B5M791_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M791"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019367"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019605.1">
        <property type="protein sequence ID" value="ENSXCOP00000019367.1"/>
        <property type="gene ID" value="ENSXCOG00000014555.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183208"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000082">
        <property type="entry name" value="Putative zinc finger E-box-binding homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008598">
        <property type="entry name" value="Di19_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051574">
        <property type="entry name" value="ZnF_E-box_Homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24391">
        <property type="entry name" value="HISTONE H4 TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24391:SF11">
        <property type="entry name" value="ZINC FINGER E-BOX-BINDING HOMEOBOX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05605">
        <property type="entry name" value="zf-Di19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017329345" description="C2H2-type domain-containing protein" evidence="13">
        <location>
            <begin position="26"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="205"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="276"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="369" mass="41893" checksum="E8FB3542745C8D0E" modified="2018-12-05" version="1">MEIQKKSWFQMFYQLSYLLLHLCVSVLNYDSVLDTTSETDEDDQTLLRDDSSFTGGAGDDEEAGSPVAVPALEASPRVGHALLSNREPDDSEHQGEDECIGESLIGSAPKCCFEYDALGPEASLQAVEDGRRLDGISGLDEFFLKRKLEDDDGHQATIAEYLQRSDTAIIYPEAPEEVTRLSTPEAGGQDENLPSSATDDFAQLLTCPYCERGYKRLTSLKEHIKYRHENTEETFTCLLCAETFNQRAQLERHMTTHKPTVDQPPLLSEGAANRKFKCSECGKAFKYKHHLKEHLRIHSGEWSRGVEEQINTHFYHPGHWHNTRHTHSEKEPRQCSLLVIKTQYPVVIRKYTKSITATVSFIQIKKNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9N7</accession>
    <name>A0A3B5M9N7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase</fullName>
            <ecNumber evidence="2">3.2.2.6</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ADP-ribosyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.6" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9N7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020235"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020483.1">
        <property type="protein sequence ID" value="ENSXCOP00000020235.1"/>
        <property type="gene ID" value="ENSXCOG00000015170.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017291"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061809">
        <property type="term" value="F:NAD+ nucleosidase activity, cyclic ADP-ribose generating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016849">
        <property type="term" value="F:phosphorus-oxygen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030890">
        <property type="term" value="P:positive regulation of B cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.82.10">
        <property type="entry name" value="ADP Ribosyl Cyclase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003193">
        <property type="entry name" value="ADP-ribosyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10912">
        <property type="entry name" value="ADP-RIBOSYL CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10912:SF9">
        <property type="entry name" value="ADP-RIBOSYL CYCLASE_CYCLIC ADP-RIBOSE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02267">
        <property type="entry name" value="Rib_hydrolayse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52309">
        <property type="entry name" value="N-(deoxy)ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017384019" description="ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase" evidence="7">
        <location>
            <begin position="25"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="208" mass="23046" checksum="E04B2779CE7C577D" modified="2018-12-05" version="1">PELSSLSAGLLLLAVVVVVPPATLLSPRTSQFRATFRQKCQAFPETSQSCSVLLSMFEEAYVGKDPCNFPDEAYHPLFVENPFIHSCDKTMFWSDTKDLVHQFCKGRNHFVSLENSLLGNIMDGLTWCGKKSSKDTFFYLCEKCDINTVSSFWVRASAEFADYACGNASVLLNGAISKPFDPTSGAFYLQERLQCCATCCTWNCGSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9Z0</accession>
    <name>A0A3B5M9Z0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9Z0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020362"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020609.1">
        <property type="protein sequence ID" value="ENSXCOP00000020362.1"/>
        <property type="gene ID" value="ENSXCOG00000015253.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158672"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110158">
        <property type="term" value="C:calpain complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46735:SF3">
        <property type="entry name" value="CALPAIN SMALL SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46735">
        <property type="entry name" value="CALPAIN, SMALL SUBUNIT 1 A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="57"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="164" mass="19052" checksum="72A704E5143E390F" modified="2018-12-05" version="1">MEISVRELRTVLNRVVSKHRDLQTDGFSMESCRAMVSLMDKDGSARLGLLEFQILWNKIRKWLGIFREFDLDKSGCMNSYEMRLALENGGFKLNNKLYQMLIARYADNEIIDFDNFTCCLVKLETMFRIFQGLDRDGTGTVEMNLMEVPNPVLSWFCSSGLKYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAF3</accession>
    <name>A0A3B5MAF3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Germ cell associated 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GSG1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAF3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021493.1">
        <property type="protein sequence ID" value="ENSXCOP00000021233.1"/>
        <property type="gene ID" value="ENSXCOG00000015888.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244814"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012478">
        <property type="entry name" value="GSG-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050579">
        <property type="entry name" value="PMP-22/EMP/MP20-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671">
        <property type="entry name" value="EPITHELIAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10671:SF43">
        <property type="entry name" value="GERM CELL-SPECIFIC GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07803">
        <property type="entry name" value="GSG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="303"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="310"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="321"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="40059" checksum="11729A64DF82BFA0" modified="2018-12-05" version="1">LAKFILSKVRSPSLSFFQTVVSLLLSTVALTSSFWCTGQQKVPKPLCTATKFKRCIPVPGVSNTSNIQFFWETGDDRFVFPNFHTGLFIICEENIYVDEWGERARVLSVEKRGAEQAIIWLCLMLEFMYISLLLVSCLLLSAQLCITAWFPSTLRWGQMLNAFAAVFTVLGGLLGMVGHMMFMQVFQTTVSMGPEDFKPHSYGYSWAFYLVWLAFTVCMVAGVSTLNNYTKKVLMVGPRSSSSLNPCSFNFVGLLPPAPYYTPPNLPVTLSAPPPAPVISHLSPYYEPPPPASPRLTHSHSLPLTHLDSFPPPPPHPAPASPFHRLSLSSPSLSSSISVHRMLPGHREHPFDRDEDYSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAZ6</accession>
    <name>A0A3B5MAZ6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Si:dkey-103g5.3</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAZ6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017096.1">
        <property type="protein sequence ID" value="ENSXCOP00000016879.1"/>
        <property type="gene ID" value="ENSXCOG00000012728.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166045"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002">
        <property type="entry name" value="ENDOGLIN/TGF-BETA RECEPTOR TYPE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14002:SF14">
        <property type="entry name" value="SI:DKEY-103G5.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="ZP" evidence="3">
        <location>
            <begin position="7"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="301" mass="33508" checksum="0DCD56677AB6EDB0" modified="2018-12-05" version="1">NSDLTIECSTTMITIEVNLCTALWAGFNASNLALNGNHNNTACLGSIDDSVEPPIIRYYLPVNNSVDNSCRQSIVDETPDPSGPFSSFSSIQTVIISSYIDTPKTDSGIISYSTDLYYYFSCSYPLEYLINNTQIVSSVSVATTNNNGSFIDTLKMGVFNDTDYRYPLTVPSSGLELRTKVYVEVKAVNLTGFHILLDHCFATPSPYNMTQTDQYNFFTTIITSNGLSNVARFNFEAFRFVQHRNQEKSTIYLHCILRLCEPTKCQELLNQACNARRKRSLTPFGEESSNSATVSVGPLYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MB48</accession>
    <name>A0A3B5MB48_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">IF rod domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MB48"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016929"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017148.1">
        <property type="protein sequence ID" value="ENSXCOP00000016929.1"/>
        <property type="gene ID" value="ENSXCOG00000012766.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155258"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000002">
        <property type="entry name" value="Type I keratin 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF347">
        <property type="entry name" value="KERATIN 93-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="7">
        <location>
            <begin position="88"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="85"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="297"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="350"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="398" mass="44442" checksum="5A83D077AA5FDAA5" modified="2018-12-05" version="1">LSNSTEPLSSQLPNGPLSSGPSVITKQTRSYTSSAAPHKAHSVSGLSFRSGPRISSSSARMVSSGFGGMGGGFDLSKGLDQSTVHLNEKATMQNLNDRLANYLEKVRSLEAANAKLEKQIREYYEKKGPAAVKDYSNYWAIINDLKDKINAATIENANILLQIDNSKLAADDFKTKFEHELIMRQSVEADIANLRRLLDQTTLTKADLEMQIEGLQDELAYLKKNHAEELAAMRSQLTGTVNVEVDAAPQQDLNKVMEEIRAQYEAITDKHRRDQEAWFNEKSAALNKEVAISTETIQTSKTEISDLRRTLQGLEIELQSQLSMKGALEHTLAETEARYSSMLCGYQNTINMLEAELSNVRASIEQQGQEYKMLLDIKTRLEQEIATYRKLLDSEESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBC9</accession>
    <name>A0A3B5MBC9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Si:dkeyp-69e1.8</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBC9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021154.1">
        <property type="protein sequence ID" value="ENSXCOP00000020897.1"/>
        <property type="gene ID" value="ENSXCOG00000015646.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165343"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF167">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="AIG1-type G" evidence="6">
        <location>
            <begin position="46"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="186"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="192"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="309" mass="33712" checksum="76CD38C12C352795" modified="2018-12-05" version="1">AELRLVVLGPIKPGNRTAVCSILGLEESRRDAEARECTKHRGEAAGRQHISSLIASSSPGPHAFLLCVPVNEPARDESMALDALAKLFGPAAVSGRTVVLFTHTEELGEDETLEQYVVTWRKDLRQLVERCGDRYHTLESGGGAAEEGRAVEELLEKVEEAVKESGGKHLSCPLYREAEERVRQRQTEIVRQRSTNSSASNSQQEVTEEEMEAVRDEAERSTGDFDLNLEDIFPSDDKSVSPSSSAPPSLLRGLREKLVDWVRWLSSLVRVEALFGSLVGLFVGGAMGGTVGATVGSVATEVSRRKTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBP8</accession>
    <name>A0A3B5MBP8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBP8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017124"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017344.1">
        <property type="protein sequence ID" value="ENSXCOP00000017124.1"/>
        <property type="gene ID" value="ENSXCOG00000012910.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155308"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099572">
        <property type="term" value="C:postsynaptic specialization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005026">
        <property type="entry name" value="SAPAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12353:SF32">
        <property type="entry name" value="DISKS LARGE-ASSOCIATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12353">
        <property type="entry name" value="DISKS LARGE-ASSOCIATED PROTEIN DAP SAP90/PSD-95-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="162"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="346"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="503"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="538" mass="58839" checksum="3CB28CA3F56B7AD6" modified="2018-12-05" version="1">MKGLGASRSRHLSDSCEPAGCAQKPLCPLTSDPHSSFLLSPTINHYGTLDPHLHHYTPTSPTSLTPDCLLPFSQVPNSSTFPRLHFTPHSDQLDCSQTAAKMNRLPSSLLDQLERHLPLQRDGFSTLQFHRGRMSKQRSESPGRIRHIMHSVQKLFAKSQSLENSGLKGSLNSTGGGEEGGRPSRRSKSKDRAKTEGSKQRPRPNTLGLWGSDDALDTDTTKAGTIAVGYRNPLSMMTLGRAVSDSQTALRHIPQGYNTISAHSLKTSKSSSDLKFLACPAINRGSKDDMSVKRGTWSTLTLNQARQVLQKGSATVNRTLLKTKSCHQDLAQQFLQVPLEDWTGNLGHGRTKGTEIPCRRMRSGSYVKAMGDPEDSEESEGSPKPSPKSAARRQSYLKATQSSLSEHLIIKFPTHSSLPSLKELPTNRSLDNLDCLVSPVELALCPQCVSLHTFFQVFAQGCGHSLPYCEGESKAVEALDLPAPTCFRSRSHSYLRAIQAGCSQDEDTTSVDSETPPPTAVGYSYSSNTSEYHLEEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MC40</accession>
    <name>A0A3B5MC40_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NACHT LRR and PYD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MC40"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021447.1">
        <property type="protein sequence ID" value="ENSXCOP00000021187.1"/>
        <property type="gene ID" value="ENSXCOG00000015855.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01070000253760"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="11125" checksum="769402FA5FD4E3E4" modified="2018-12-05" version="1">MSEVSCSSLVSALKSNPSHLTELDLRGNDLKDSGVKELCGFLQNPECRIQRLMSCSLSEISCSSLVSALKSNQHLTELDLRGNNLKDSDVQQLKLFVKDVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEG4</accession>
    <name>A0A3B5MEG4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Estrogen receptor binding site associated antigen 9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027874304.1">
        <property type="nucleotide sequence ID" value="XM_028018503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEG4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022658"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022934.1">
        <property type="protein sequence ID" value="ENSXCOP00000022658.1"/>
        <property type="gene ID" value="ENSXCOG00000016931.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114145111"/>
    <dbReference type="KEGG" id="xco:114145111"/>
    <dbReference type="CTD" id="9166"/>
    <dbReference type="GeneTree" id="ENSGT00390000004040"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017025">
        <property type="entry name" value="Cancer-assoc_antigen_RCAS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15208:SF2">
        <property type="entry name" value="RECEPTOR-BINDING CANCER ANTIGEN EXPRESSED ON SISO CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15208">
        <property type="entry name" value="RECEPTOR-BINDING CANCER ANTIGEN EXPRESSED ON SISO CELLS CANCER ASSOCIATED SURFACE ANTIGEN RCAS1 ESTROGEN RECEPTOR-BINDING FRAGMENT- ASSOCIATED GENE 9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF034247">
        <property type="entry name" value="RCAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017230394" description="Estrogen receptor binding site associated antigen 9" evidence="4">
        <location>
            <begin position="28"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="170"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR034247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="24292" checksum="D1C0015024E46E85" modified="2018-12-05" version="1">MAITQFRLFKICTCLASLLSFFKRLICRSGRGRKLSGDQITLPTTVDFSSSLAKQPEIEEWSSWDEEAPTSIKIEGGNGNVSPPPNDADEEPDYFKDMAPTIRKTQKIVLKKREPLNYLVPDGSAGFSSRLAASQDMMSFSPPSGELGEMENWQEDTNAWEDESDAAWEAEEVLRQQKMAEREKRFMEQQKKKMEKEAQRMLKKEQKIAVKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIK0</accession>
    <name>A0A3B5MIK0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIK0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019384"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019622.1">
        <property type="protein sequence ID" value="ENSXCOP00000019384.1"/>
        <property type="gene ID" value="ENSXCOG00000014567.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633">
        <property type="entry name" value="IMMUNOGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47633:SF4">
        <property type="entry name" value="MYOPALLADIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="45"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="151" mass="16545" checksum="78F23919C34E90AE" modified="2018-12-05" version="1">MFLRSTSPRSLSRSPGRSPSRSPGRSPARRLDETDEQQLERLYKPVFVMKPTACKCSEGQTARFDLKVVGRPMPDTYWFHNGETHIKGIFIEEDGTQSLIIVPAMPHDSGEWTVVAQNRAGRSSISVTLTVDGKTSLSSGDGEAAGEHRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MK41</accession>
    <name>A0A3B5MK41_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MK41"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019884"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020131.1">
        <property type="protein sequence ID" value="ENSXCOP00000019884.1"/>
        <property type="gene ID" value="ENSXCOG00000014931.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
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        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="3">
        <location>
            <begin position="13"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="145" mass="15674" checksum="92B3C617AEF19C4F" modified="2018-12-05" version="1">MNGSLPSCPRLVDTNLKRGLAGQKFNINGGINQQFIVNDNGVYVGRVKKDGSAALDGRLEDGDKILVINGVLLENRYAGEKVERRKKSHLSTGPPTSKPNRQPSTSTLVILVLFARAASIIVIVALYKRKCKGPGGLYENDPVCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKD9</accession>
    <name>A0A3B5MKD9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKD9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021870.1">
        <property type="protein sequence ID" value="ENSXCOP00000021606.1"/>
        <property type="gene ID" value="ENSXCOG00000016156.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000235259"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327">
        <property type="entry name" value="RING FINGER PROTEIN 127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327:SF51">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR OF YEAST FORM ADHERENCE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="15"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="74" mass="8470" checksum="B45F7C7EE02B4F01" modified="2018-12-05" version="1">MATSCDFLSEDQFQCSICLDIFTDPVSTPCGHTFCKACLTRHWAGKRECQCPLFNNQIQKCFGGIFWFLTLLHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5ML07</accession>
    <name>A0A3B5ML07_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5ML07"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021821"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022085.1">
        <property type="protein sequence ID" value="ENSXCOP00000021821.1"/>
        <property type="gene ID" value="ENSXCOG00000015757.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183165"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000005">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000002">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="12">
        <location>
            <begin position="54"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="13">
        <location>
            <begin position="253"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="454" mass="50398" checksum="587B8E1D950ABBC5" modified="2018-12-05" version="1">MVSSSQRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPSVIDEVRSGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSVYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNSCFEPANQLVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGADSLGDEDEGEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLD1</accession>
    <name>A0A3B5MLD1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G protein-coupled receptor kinase</fullName>
            <ecNumber evidence="11">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[G-protein-coupled receptor] + ATP = [G-protein-coupled receptor]-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12008"/>
            <dbReference type="Rhea" id="RHEA-COMP:11260"/>
            <dbReference type="Rhea" id="RHEA-COMP:11261"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLD1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025204"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025509.1">
        <property type="protein sequence ID" value="ENSXCOP00000025204.1"/>
        <property type="gene ID" value="ENSXCOG00000018830.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158544"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050254">
        <property type="term" value="F:rhodopsin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05605">
        <property type="entry name" value="STKc_GRK4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000009">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000074">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000239">
        <property type="entry name" value="GPCR_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF15">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00717">
        <property type="entry name" value="GPCRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RGS" evidence="13">
        <location>
            <begin position="46"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="13">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="157"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="420"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600239-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="539" mass="62147" checksum="188681E70657A41C" modified="2018-12-05" version="1">MDWRGGGGNRKGKSKKWKQMLQFPHISLCEELRQTTEKDYSSLCERQPIGRLLFRQFCDTRPELRRCVKFLDAVAEYEVTPDEKRKECGQELIDKYFNPKVCPSGFDSLNQNLTFVTVLIHDYLSVAPFADYLDSMYFNRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGESMALNEKQILEKVNSRFVVSLAYAYETKDALCLVLTLMNGGDLKFHIYHMGDAGFEERRAVFYAAEICCGLEDLHRERIIYRDLKPENILLDDHGHIRISDLGLAIRVPDGETIKGRVGTVGYMAPEVVMNERYTFSPDWWALGCLLYEMIEGQSPFQQRKKKIKREEVERLVKEVQEEYSSKFSEEAKSICKMLLAKDPTKRLGSLGGGASEVKGHPIFRPINFKRLEAGMLQPPFIPDPQAIYAKDVLDIEQFSTVKGVELDPKDESFYSKVSTGSVSIPWQNEMIETECFSELNVFYEDGAIPPDLDWRGQPTPTPKQGLLQRLFGRQVRSLKSLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MMD7</accession>
    <name>A0A3B5MMD7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MMD7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025584"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025893.1">
        <property type="protein sequence ID" value="ENSXCOP00000025584.1"/>
        <property type="gene ID" value="ENSXCOG00000019112.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157632"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="76" mass="8772" checksum="C111D570AC67EFB1" modified="2018-12-05" version="1">MGTWARTLLDHAYDEETDYCPESYTLSVQDLERDLEKNMSPKTMKRSQSVVEPGVLKRPDKVKSEEDNLQPLLSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MMI0</accession>
    <name>A0A3B5MMI0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Synovial sarcoma, X breakpoint 2 interacting protein a</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
            <location evidence="2">Centriolar satellite</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ADIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MMI0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024993"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025297.1">
        <property type="protein sequence ID" value="ENSXCOP00000024993.1"/>
        <property type="gene ID" value="ENSXCOG00000018654.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007688"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007368">
        <property type="term" value="P:determination of left/right symmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035735">
        <property type="term" value="P:intraciliary transport involved in cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052300">
        <property type="entry name" value="Adhesion_Centrosome_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021622">
        <property type="entry name" value="Afadin/alpha-actinin-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46507">
        <property type="entry name" value="AFADIN- AND ALPHA-ACTININ-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46507:SF2">
        <property type="entry name" value="AFADIN- AND ALPHA-ACTININ-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11559">
        <property type="entry name" value="ADIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="295"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="306"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="534" mass="61664" checksum="093823FE8F2787F8" modified="2018-12-05" version="1">MGEWRMQCTNYFYVFSLFSEDPEISSISHVTMSPSRHNHVPVYSLPLFRSSYSVISAFCTEDNVPQSISYLNQELAVLGLSMCIEASLSEGASLKAVPVLNAMYELLQIHKRNMCALEEMEKEQLKKLSTLEHIQISNSRLKDQLELSMREKSGLHETERQLQLKIKTLQSCLKTEKEEVQKLQSIIASRASQYSHDAKRKEREAAKLKERLSRLLIDRKEKKLAIDVLNSLGRSDGKRSMWKTAKLSASHESEMYKALLGDYETSRKALILENAELKKVLQQMKKEMMHILAPRQQSTSGAAADDSQEQVESDEEKAGNASREALDHSCELAREQLLNSIRQQWRKLRNHVEKLDQGTSVMESNKETIPLETHEDEMERMRQEVQQCREFIHAQQQLLQQQLNTSFDDETAALLNDCYTLEEKERLKEEWKLFEEQKKNFEKERKNFTEAAIRLGREKMAFEEDRVAWLKNHFLNITPFTNRRRYSSSDGQSALSGIKYATSICTCTVFISWVCAATPQDVPKKPNLQGHHCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNL5</accession>
    <name>A0A3B5MNL5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027890688.1">
        <property type="nucleotide sequence ID" value="XM_028034887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNL5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022756"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023032.1">
        <property type="protein sequence ID" value="ENSXCOP00000022756.1"/>
        <property type="gene ID" value="ENSXCOG00000016998.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114155143"/>
    <dbReference type="KEGG" id="xco:114155143"/>
    <dbReference type="GeneTree" id="ENSGT00940000164243"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046323">
        <property type="term" value="P:D-glucose import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070837">
        <property type="term" value="P:dehydroascorbic acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="MFS_GLUT_Class2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000029">
        <property type="entry name" value="solute carrier family 2, facilitated glucose transporter member 4"/>
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    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
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        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF1">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="356"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="423"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="453"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="34"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="511" mass="56063" checksum="64A6581AA78CE8A1" modified="2018-12-05" version="1">MPTEYVDEYEPLLVRGIKDERRLKLPNKSLLLAVFAACVGGTFQYGYNISVINAPTVYVQNFINQTWMQRYQTNISQGGLTLLWSTIVSMFTLGGFIGTSVGGTLSVKLGRRGTLLANNAFSLTAALLMGLSSTAGSFELLIIGRLLSGMNAGIALCVQPLYLGEIAPTALRGAMGMGTSVFITGGILTGQVMGLKEILGKEEYWPILLSTTCIPAFLQLLTLPWFPESPRYLLIDKGDEESCKKALRQLHGLADSGDALEDIEKEKNNLVGFQAKKPWELFSDRSLRWQLLTIILLNIAQQLNGINAIYFYADYVFGQSGIPMDKIRYATVGTGACECITALTCGMLIECLGRKVLIMGGYVLMSICCVFFTLTLTFQDFSPVFPYLSMACVFAFILSFGLGPGGVTNILTTELFTQNTRPAAFMIAGSVNWLSFFLISMVFPFIVIGLQQYCFLVFLAVCSLVVTYIFFVVPETKNKTFVEIQNEFQSLKTNKFCSSDGAGTILLSTSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPK2</accession>
    <name>A0A3B5MPK2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027880319.1">
        <property type="nucleotide sequence ID" value="XM_028024518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MPK2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023106"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023384.1">
        <property type="protein sequence ID" value="ENSXCOP00000023106.1"/>
        <property type="gene ID" value="ENSXCOG00000017266.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114149001"/>
    <dbReference type="KEGG" id="xco:114149001"/>
    <dbReference type="GeneTree" id="ENSGT00940000157820"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Polyubiquitin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
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        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
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    </dbReference>
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        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="77" mass="8707" checksum="16A8FA35397FFD9B" modified="2018-12-05" version="1">MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNVQKESTLHLVLRLRGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPK3</accession>
    <name>A0A3B5MPK3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MPK3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025718"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026028.1">
        <property type="protein sequence ID" value="ENSXCOP00000025718.1"/>
        <property type="gene ID" value="ENSXCOG00000019221.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074694"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05528">
        <property type="entry name" value="Bromo_AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19517">
        <property type="entry name" value="RecA-like_Yta7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000016">
        <property type="entry name" value="ATPase family AAA domain-containing protein 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000734">
        <property type="entry name" value="ATPase family, AAA domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000061">
        <property type="entry name" value="ATPase family, AAA domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045199">
        <property type="entry name" value="ATAD2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069:SF4">
        <property type="entry name" value="ATPASE FAMILY AAA DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="6">
        <location>
            <begin position="653"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="886" mass="100618" checksum="11B3545D0954783A" modified="2018-12-05" version="1">SETYLFTSSLSIRAAQNAWSTDDADSDDEKASSSNCRSLGLRTEELFSSRRDKLAMGAGLADIDPMAIDRKVGFDSIGGLSGHISALKEMVVFPLLYPEVFDNFKIQPPRGCLFYGPPGTGKTLVARALANECSHGNEKVSFFMRKGADCLSKWVGESERQLRLLFEQAYQRRPSIIFFDEIDGLAPVRSSKQDQIHSSIVSTLLALMDGLDGRGEIVVIGATNRLDSIDPALRRPGRFDREFLFGLPDKESRKEIMKIHTRLWKPPPSEDFLDELAEKCVGYCGADIRAVCTEAALCALRRRYPQIYGTSQKLLLDVSSISVSSCDFVAAMRKMKPASQRSNACPTKPLSAVVQPLLHSALSHILEVLQKMFPHAEQGMKRKREPDLTSGILDDGLMSSGDEGTSTCSISAPSTSQNKNFLHFARSAVKHPTSHRPRMLLAGRPDSGQTSHLAPAILHALERFTVHSLDSAVLFGFSWTSPEEACAQVFCEAKRTSPSILYIPHIQQWWDTAGSALRASFLSLLGSIPSFSPILLLATCSVPHQKLDTEIQTLFREEYGEIYTMTVPSRQERTEFFEDLILNQAAEPPPSKNKRMTQNMEILPVAPLPPLRQMSEQERLRVEEQEEDVLRELRLFLRNLTERLMVDRRFKAFTKPVDIEEVPDYLIVIQKPMDLSTLLYNIDEHKYVTVDEFMSDADLIWQNALEYNPDRDPADRHIRHRACALKDTMRVIVRDELDKDFQRICEEIRESRLERGKTLFTVAKLHQVFSMYSQFYSYKCYLVLNPASNLFAAIVCSHYSCFLQELLNRAIIKTEAAEVETLEKLYALLAQSIYRHRNIYNKTELIKVQQRHSALFIQEPFRWRLLKRVEKLLKAITYYCFILAVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQ18</accession>
    <name>A0A3B5MQ18_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">STAT transcription factor protein interaction domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQ18"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021579"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021843.1">
        <property type="protein sequence ID" value="ENSXCOP00000021579.1"/>
        <property type="gene ID" value="ENSXCOG00000016148.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000178909"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.532.10">
        <property type="entry name" value="STAT transcription factor, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036535">
        <property type="entry name" value="STAT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013799">
        <property type="entry name" value="STAT_TF_prot_interaction"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02865">
        <property type="entry name" value="STAT_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48092">
        <property type="entry name" value="Transcription factor STAT-4 N-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="STAT transcription factor protein interaction" evidence="1">
        <location>
            <begin position="2"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="84" mass="9968" checksum="6E47994B6B1BE0E0" modified="2018-12-05" version="1">MAQWQELLKLDSALQNQVRQLYEGRFPTEIRNQLSQWTESQDCGHWTRTNCPCCRPRSWVGPTEPEPPTSSMFLLPYMQWIRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQB6</accession>
    <name>A0A3B5MQB6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM263 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQB6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023371"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023652.1">
        <property type="protein sequence ID" value="ENSXCOP00000023371.1"/>
        <property type="gene ID" value="ENSXCOG00000017455.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013899"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028153">
        <property type="entry name" value="UPF0444"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31443">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15475">
        <property type="entry name" value="UPF0444"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="119" mass="12004" checksum="44E803697396417A" modified="2018-12-05" version="1">MKESVSSKTINRCNKSKLWLICVPDPGKDHAVAPPGMVWRVTGGLFNVTKGVVGAAVGGVAWVGGKSLEITKSAVTTVPSVGVGLVKGGVSAVAGGVSSVGSTVANKVPFTAKRKDKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MR18</accession>
    <name>A0A3B5MR18_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Liprin-beta-1/2 coiled-coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MR18"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021924"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022191.1">
        <property type="protein sequence ID" value="ENSXCOP00000021924.1"/>
        <property type="gene ID" value="ENSXCOG00000016383.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175988"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048786">
        <property type="term" value="C:presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007528">
        <property type="term" value="P:neuromuscular junction development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058914">
        <property type="entry name" value="LIPB1/2_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029515">
        <property type="entry name" value="Liprin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587">
        <property type="entry name" value="LAR INTERACTING PROTEIN LIP -RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12587:SF18">
        <property type="entry name" value="LIPRIN-BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26022">
        <property type="entry name" value="CC_Liprin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Liprin-beta-1/2 coiled-coil" evidence="3">
        <location>
            <begin position="92"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="88"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="214" mass="24061" checksum="AFE3842399A1D5BE" modified="2018-12-05" version="1">METHASHMLKAALEQMDDIIAGKNVDPELPLPTPGDPAFVALELMEVLRAVLEDQRSEEKQGALSKQMSTDTANVILKWLQRDEVSNSESYQERLSRLEGDKESLILQVSVLTDQVEAQGVKIHDLESSLVEHQHKLNSTEEMLQQELVHRTSLEKQKLNLMGEVSYLKLKLADMEGTQVHGAERQHKAEVRLLTGSGTPQVHYIDNFGFIILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRT1</accession>
    <name>A0A3B5MRT1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">RNA binding motif protein 24b</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRT1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026493"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026814.1">
        <property type="protein sequence ID" value="ENSXCOP00000026493.1"/>
        <property type="gene ID" value="ENSXCOG00000019779.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156245"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003228">
        <property type="term" value="P:atrial cardiac muscle tissue development"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:embryonic neurocranium morphogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:heart looping"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0006397">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:mRNA stabilization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of myotube differentiation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007519">
        <property type="term" value="P:skeletal muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001756">
        <property type="term" value="P:somitogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003229">
        <property type="term" value="P:ventricular cardiac muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd12384">
        <property type="entry name" value="RRM_RBM24_RBM38_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding motif protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050886">
        <property type="entry name" value="RNA-binding_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024">
        <property type="entry name" value="GEO13361P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024:SF59">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN 24B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="10">
        <location>
            <begin position="11"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="238" mass="25147" checksum="E1783EED880A623B" modified="2018-12-05" version="1">MHSAQKDTTFTKIFVGGLPYHTTDSSLRKYFEVFGDIEEAVVITDRQTGKSRGYGFVTMADRASADRACKDPNPIIDGRKANVNLAYLGAKPRVIQPGFAFGVPQIHPAFIQRPYGIPAHYVYPQAFVQPSVVIPHVQPSAATATAAAAAATSPYLDYTGAAYAQYSAAATAAAAAAAAAYDQYPYAASPAPTSYMTTAGYGYTVQQPLATAATPGAAAAAAAFSQYQPQQLQTDRMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSU7</accession>
    <name>A0A3B5MSU7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small ribosomal subunit protein eS19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">40S ribosomal protein S19</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Required for pre-rRNA processing and maturation of 40S ribosomal subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSU7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026566"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026888.1">
        <property type="protein sequence ID" value="ENSXCOP00000026566.1"/>
        <property type="gene ID" value="ENSXCOG00000019835.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013102"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000255">
        <property type="entry name" value="40S ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_eS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018277">
        <property type="entry name" value="Ribosomal_eS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00628">
        <property type="entry name" value="RIBOSOMAL_S19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00058881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="154" mass="17065" checksum="60AE77A9E578444C" modified="2018-12-05" version="1">MPSVTVKDVNQQEFVKALSAFLKKSGKLKVPEWVDTVKLARHKELAPCDDNWFYTRAASTARHLYLRGGVGVGAMIKIYGGRQRNGVCPAHFSVGSRNVARKVLQALEGLKMVEKDPSGGRRLSPQGQRDLDRIAGQVSAACCRRPRWGTCSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVQ7</accession>
    <name>A0A3B5MVQ7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Potassium voltage-gated channel, shaker-related subfamily, member 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVQ7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027923"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028259.1">
        <property type="protein sequence ID" value="ENSXCOP00000027923.1"/>
        <property type="gene ID" value="ENSXCOG00000020823.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158576"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044224">
        <property type="term" value="C:juxtaparanode region of axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033270">
        <property type="term" value="C:paranode region of axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000002">
        <property type="entry name" value="Potassium voltage-gated channel subfamily a member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000007">
        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000021">
        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003972">
        <property type="entry name" value="K_chnl_volt-dep_Kv1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
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        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
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        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF24">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY A MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01496">
        <property type="entry name" value="SHAKERCHANEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="POZ domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="7" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="19">
        <location>
            <begin position="26"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="419"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="419"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="435"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="475" mass="54048" checksum="851AE19C966A22BF" modified="2018-12-05" version="1">MDETSAVPGHPQDTYPPDHNDHECCERVVINIAGLRFETQLKTLSQFPETLLGNPKKRMRYFDPLRNEYFFDRNRPSFDAILYYYQSGGRLRRPVNVPLDMFSEEIKFYELGVDAMERFREDEGFIREEERPLPEKEFQRQIWLLFEHPESSGPARGIAIVSVMVILISIVIFCLETLPQLKEDPMGRIETFGNITIRYKPNILTDPFFVIETLCIIWFSFELIVRFMACPSKPAFFKNMMNTIDIVAIIPYFITLGTELAEDPENEGVGEQATSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILFSSAVYFAEAEEPDSHFGSIPDAFWWAVVSMTTVGYGDMVPVTIGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETEGEEQAQLLNVSNPNIPSETNSSRHDPPTPNPSPPRSHNPFPSPPAKDLLLSKAPYSRKTALLSCFWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MW82</accession>
    <name>A0A3B5MW82_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Death effector domain-containing 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MW82"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025637"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025946.1">
        <property type="protein sequence ID" value="ENSXCOP00000025637.1"/>
        <property type="gene ID" value="ENSXCOG00000019154.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008714"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008625">
        <property type="term" value="P:extrinsic apoptotic signaling pathway via death domain receptors"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="DED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038856">
        <property type="entry name" value="DEDD/DEDD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049341">
        <property type="entry name" value="TRADD-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15205">
        <property type="entry name" value="DEATH EFFECTOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15205:SF1">
        <property type="entry name" value="DNA-BINDING DEATH EFFECTOR DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01335">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRADD-like_N"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50168">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
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    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DED" evidence="7">
        <location>
            <begin position="27"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="267" mass="30429" checksum="DE96FF8AC8C86187" modified="2018-12-05" version="1">IELKATTPMDSLHWDETECLNYYGMLSLHEMFDVVASQLTESDIEALSFLLNETYPAQHPLDPFKSGLELLLELERRGYLYDGNLEPLLQLLRVLTRHDLLPFVSHKKRRTEKTVATKTSRLVQPLPLPLGPQKVSCDIRLRVRAEYLEHESALREGVSSNKRQPLERQFELFSRSSSLLRARDLGSIVCDIKFTELDNLEAFWADYLSGALLEALKGVFITDSLRTAAGSEGVRLLISVDQDDYEEGRRLMKLEGGRIFFDAARNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWW9</accession>
    <name>A0A3B5MWW9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA polymerase II elongation factor ELL N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWW9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024309"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024602.1">
        <property type="protein sequence ID" value="ENSXCOP00000024309.1"/>
        <property type="gene ID" value="ENSXCOG00000018157.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164943"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031176">
        <property type="entry name" value="ELL/occludin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019464">
        <property type="entry name" value="ELL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288">
        <property type="entry name" value="OCCLUDIN AND RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288:SF12">
        <property type="entry name" value="RNA POLYMERASE II ELONGATION FACTOR ELL2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10390">
        <property type="entry name" value="ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase II elongation factor ELL N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="79" mass="9047" checksum="CD30C277AEECF451" modified="2018-12-05" version="1">MASLRQEHRYGLSCGKTTKNSSNITLYHVKLTDTAIRALEAYQNLKGVLQNETSICFKGNHGVSFFILFYYYFILISMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXH9</accession>
    <name>A0A3B5MXH9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Galectin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXH9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026357"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026677.1">
        <property type="protein sequence ID" value="ENSXCOP00000026357.1"/>
        <property type="gene ID" value="ENSXCOG00000019683.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155727"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00070">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000124">
        <property type="entry name" value="Galectin-4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044156">
        <property type="entry name" value="Galectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346:SF111">
        <property type="entry name" value="GALECTIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00276">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0430">Lectin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Galectin" evidence="4">
        <location>
            <begin position="17"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Galectin" evidence="4">
        <location>
            <begin position="186"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU102079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="316" mass="35204" checksum="9B50B78FD5F409EB" modified="2018-12-05" version="1">MSISNPRQVFSNPLIPFAGTILGGLLPDEMVLIQGSVPSGADRFQVDFTCGSSVKPRADVAFHFNPRLKKKRIVCNTLEKERWGSEEILYQMPLRVGAAFELIFLVLKEKYKVAVNGAHVLEYKHRVDLNRVDTILISGAVAVQAVAVLPPASSPASPASSLTIKMKNTNMQTQNSVFRLHQRFPFRGRLEKGLKVGHSIIIKGQTSSNAKSFAVNLRDASGRDIVLHLNPRLAKRVFARNSFLSECWGAEESDLEAFPFAAGEYFEIIVRSNERHFRVAINGCHQFDYKHRVQDLSAVGQLEVVGDVTLMDVRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXT8</accession>
    <name>A0A3B5MXT8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the potassium channel HCN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027892958.1">
        <property type="nucleotide sequence ID" value="XM_028037157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXT8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026884.1">
        <property type="protein sequence ID" value="ENSXCOP00000026562.1"/>
        <property type="gene ID" value="ENSXCOG00000019831.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114156620"/>
    <dbReference type="KEGG" id="xco:114156620"/>
    <dbReference type="CTD" id="57657"/>
    <dbReference type="GeneTree" id="ENSGT00940000154743"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098855">
        <property type="term" value="C:HCN channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005272">
        <property type="term" value="F:sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003254">
        <property type="term" value="P:regulation of membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000002">
        <property type="entry name" value="Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013621">
        <property type="entry name" value="Ion_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051413">
        <property type="entry name" value="K/Na_HCN_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003938">
        <property type="entry name" value="K_chnl_volt-dep_EAG/ELK/ERG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689">
        <property type="entry name" value="I[[H]] CHANNEL, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45689:SF7">
        <property type="entry name" value="POTASSIUM_SODIUM HYPERPOLARIZATION-ACTIVATED CYCLIC NUCLEOTIDE-GATED CHANNEL 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08412">
        <property type="entry name" value="Ion_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01463">
        <property type="entry name" value="EAGCHANLFMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0114">cAMP</keyword>
    <keyword evidence="6" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="9" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="9" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0915">Sodium</keyword>
    <keyword evidence="4" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="15" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="154"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="308"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="22">
        <location>
            <begin position="488"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="621" mass="72064" checksum="D9C21B734A4651D2" modified="2018-12-05" version="1">MTSPSRSIDDQRGTGESPAHKAETSRYRSWSSLRFSRWRSSSQRTTPPSTPSPKTEKRSDISRTLFTLVKTHNDDYTADPDGSLDMGAEAEVGLDDLLQDQSTYFQKQFGSMLQPGVNKFSLRMFGSHKGVAAEQERVKSFGVWIIHPYSDFRFYWDIVMLLLMMGNLIILPWGITFFEDQNTIPWIIFNFLSDTLFLMDLVFNFRTGIPGEDSHIILDPKEIRMHYLRTWFLVDFVSSIPVDYIFLIVDLESRHESSDVYRTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIVNLIGMMLLLCHWDGCLTFMVPMLQDFPPDCWVSKNNMVNSTWHIQYSYALFMAMSHMLCIGYGAHPPEGMTDVWLTMISMVVGATCYAMFLGHATNLVQSLDASHRQYQEKYKQVEQYMSFHKLPADVRQRIHDYYEHRFQGKMFDEDSILGELSDPLKEEIVSYNCRGLVANMPLFANTDPHFVTVILTKLRFEVFQPGDYVIREGTLGRKMYFIQHGTVTIIPRGSKELMLSDGAYFGEICLLTQGRRTASVRADTYCRLYSLSVDDFNEVLEEHPLMRRAFESVAVERLDRVSRRSSYQPPTTESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXU9</accession>
    <name>A0A3B5MXU9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXU9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026911.1">
        <property type="protein sequence ID" value="ENSXCOP00000026587.1"/>
        <property type="gene ID" value="ENSXCOG00000019851.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000271516"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="58" mass="6705" checksum="711D198F4E89670B" modified="2018-12-05" version="1">LVAEQELRPEALQLRFLRLRHPVQDGVFFPFEHLQFDLIVGDFGFVFGFCFNGGFLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MY36</accession>
    <name>A0A3B5MY36_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MY36"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025090"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025395.1">
        <property type="protein sequence ID" value="ENSXCOP00000025090.1"/>
        <property type="gene ID" value="ENSXCOG00000018733.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156845"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072673">
        <property type="term" value="P:lamellipodium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046987">
        <property type="entry name" value="Myo9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46184:SF2">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IXB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46184">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IXB-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 8" id="KW-0518">Myosin</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-associating" evidence="9">
        <location>
            <begin position="15"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="10">
        <location>
            <begin position="123"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="217"/>
            <end position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="329" mass="36766" checksum="6D67D7EDB59380E1" modified="2018-12-05" version="1">MSATDGAGTPEHDKDARVVQIYPRLPLETDAYCPLQVSIKDTVKTVIHNAVVTLDLDSSQSYSLLEVKESSGEETPLDDGDRPLVRVLRWPPETQKWHPRSQGYYFVLQQSNNGAKQVATSKEESDDLCSLPSVTKEAVLEVLRQRFHKHKIYTYISNILVAVNPNRFLPLYYNPKYVKLYKNQPLGNLSPHIFAIADVAFRTMLDRQANQCILISGESGSGKTESSSYLIHCLTTLNPGTYAGGLERAILGAGPVLEAFGNAKTAENNNSSRFGKFIQLIYLESGVIRGAFVEKFLLEKNRLVSRAKNQRYAILNLQIINSDYDPNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MY98</accession>
    <name>A0A3B5MY98_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ashwin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ashwin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MY98"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025220"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025525.1">
        <property type="protein sequence ID" value="ENSXCOP00000025220.1"/>
        <property type="gene ID" value="ENSXCOG00000018839.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007488"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072669">
        <property type="term" value="C:tRNA-splicing ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024887">
        <property type="entry name" value="Ashwin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28359">
        <property type="entry name" value="ASHWIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28359:SF1">
        <property type="entry name" value="ASHWIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15323">
        <property type="entry name" value="Ashwin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="57"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="144"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="159"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="209" mass="23151" checksum="B7F7F009A05D5049" modified="2018-12-05" version="1">MAASTGQNKESVSTSDADLLLHPELLSQDFMRLILKEVNRDRLTELYLRHVIPLPQRTLPNNRWGRRMQKSRGRQTAAGHRSERSSSCNDQHRKRPLIVFDGKSSQSGPLKVKKPEGTKVSAGVTDRLKPPPAANLSNPIRKLSGTSSSSSSSTHRGSETANLKQDTGDSVGSYSDRLEILKINSKYCTITGALKSPEVKKKIQHITWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MYX4</accession>
    <name>A0A3B5MYX4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MYX4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027262"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027595.1">
        <property type="protein sequence ID" value="ENSXCOP00000027262.1"/>
        <property type="gene ID" value="ENSXCOG00000020360.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064743"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017171">
        <property type="term" value="F:serine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008547">
        <property type="entry name" value="DUF829_TMEM53"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20908">
        <property type="entry name" value="LD15586P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20908:SF4">
        <property type="entry name" value="SI:DKEY-5I3.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05705">
        <property type="entry name" value="DUF829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="281" mass="32231" checksum="9F284DD0C63CA7C1" modified="2018-12-05" version="1">MIARTSLSRGITAHRLCKNVTLYLNELTSPGVMVQSQTPEDHKPLMLMLPWLGSRPQAVSKYCEIYFRTGFDVLVVESEVKDFLWPRWGLDRGKTLLELLQSERFMSRPLLVHAFSIGGYTFTQLLVHISQDKQQYQAVTNRIKGQVYDSLVIGSLEHMATGLGKTLYPRLENLVVQASMLYFCIFKRQTVDYFNASIDAFYNTPVKAPALIFFCENDLLSDARSVEKLVDYWQNCGMDITTKKWEVSTHAGHLRQHPQEYLSTLNTFLNSLHIVPLKAKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KM78</accession>
    <name>A0A3B5KM78_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10a</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KM78"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001218"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001236.1">
        <property type="protein sequence ID" value="ENSXCOP00000001218.1"/>
        <property type="gene ID" value="ENSXCOG00000000994.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159462"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.310.10:FF:000001">
        <property type="entry name" value="Serpin family A member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF191">
        <property type="entry name" value="PROTEIN Z-DEPENDENT PROTEASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017212672" evidence="3">
        <location>
            <begin position="24"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="4">
        <location>
            <begin position="42"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="396" mass="42783" checksum="74078829B073BCE7" modified="2018-12-05" version="1">KTMAAFLFLHLAFLFLLAGPAAPQTPDPAELDLLNRSADFGARLYRAVAGRTDDNVLLAPLAVFEGMLALLGGTSGPTQNELLQGLTLTGLDPQTLPGGLRPQHDPEEDGPPSQGAALFLDQSFQAPPSFQDLVQTKYGGQTQSVSFSSPADAQDVINRWVQDRTRHQAQNLLSALDRRTQLLLVSAADYQSDPPFNASLTQVERFYVDRYHVAMVPMMFRADKYFLAYDSNVKAGVLKLPMADGAAMLAVLPDEDVDLTAVEGEVTSEKIRAWIRQLKKTKLEVQLPVFLLKRSYALKDVLQALYVTQVFQDDADLSSMGGASGASLSQVSGPVYHASVVSMDESGDDGGSGGGATAFSSPPPRLTFNRPFIFIIYQQASGSLLLMGRVANPTEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KS04</accession>
    <name>A0A3B5KS04_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">N(6)-adenosine-methyltransferase non-catalytic subunit METTL14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3 6">Methyltransferase-like protein 14</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis. In the heterodimer formed with mettl3, mettl14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer; heterodimerizes with mettl3 to form an antiparallel heterodimer that constitutes an active methyltransferase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the MT-A70-like family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027874976.1">
        <property type="nucleotide sequence ID" value="XM_028019175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KS04"/>
    <dbReference type="SMR" id="A0A3B5KS04"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001262"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001280.1">
        <property type="protein sequence ID" value="ENSXCOP00000001262.1"/>
        <property type="gene ID" value="ENSXCOG00000000993.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114145551"/>
    <dbReference type="KEGG" id="xco:114145551"/>
    <dbReference type="CTD" id="57721"/>
    <dbReference type="GeneTree" id="ENSGT00550000075003"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140640">
        <property type="term" value="F:catalytic activity, acting on a nucleic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016556">
        <property type="term" value="P:mRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045123">
        <property type="entry name" value="METTL14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13107">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13107:SF0">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51592">
        <property type="entry name" value="SAM_MTA70L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="2 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="51"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="393"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="407"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="423"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00032942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00049757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="455" mass="52411" checksum="6604EE73F9ADC5B4" modified="2018-12-05" version="1">MNSRLQEIRERQKLRRQLLAQQLGAESADRIGVVLHSKDELKEIEETREICRASYDSSAPPSKKKPQTEGEDTEEDVEEQKDEVEVQQEEESNPYEEVYKDSSTFLKGTQSLNPHNDYCQHFVDTGHRPQNFIRDVGLADRFEEYPKLRELIRLKDEHISSTNIPPMYLQADPEHFDLRELKSEFDVILIEPPLEEYYRESGISHTERFWTWDDIMRLEIEEISALRSFVFLWCGSGEGLDLGRMCLRKWGFRRCEDICWIKTNKNNPGKTKALDPKAVFQRTKEHCLMGIKGTVRRSTDGDFIHANVDIDLIITEEPEMGNVEKPVEIFHIIEHFCLGRRRLHLFGRDSTIRPGWVTVGPTLTNTNFNPETYASHFPMPDSHLSGCTEEIERLRPKSPVKLKSDRGGGAPRGGRGGPNSGRGGDRGRERNRPNFRGDRGGFRGRGGPHRGFPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KSD2</accession>
    <name>A0A3B5KSD2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KSD2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000840"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000850.1">
        <property type="protein sequence ID" value="ENSXCOP00000000840.1"/>
        <property type="gene ID" value="ENSXCOG00000000711.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168128"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="49" mass="5351" checksum="579AA1011AF9613A" modified="2018-12-05" version="1">KERQENESVSAAPPDGPSQLDTLSKDDLIKYAKKQIAAMQKMKTKCAGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KSU5</accession>
    <name>A0A3B5KSU5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KSU5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001033.1">
        <property type="protein sequence ID" value="ENSXCOP00000001020.1"/>
        <property type="gene ID" value="ENSXCOG00000000837.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155662"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033562">
        <property type="entry name" value="PLPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12406:SF22">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE PNPLA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12406">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2 IPLA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="4">
        <location>
            <begin position="8"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="2">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="43"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="157"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="249" mass="27713" checksum="504912A10F4743B5" modified="2018-12-05" version="1">PAVVGWSISFAGCGFMGIYYVGVISCILQRFPRFIRDASKIYGASAGALMAAIITLGIPLGEWGFSPARKHKLGPLHPAYNLLQLVQESLLEKLPEDAHLRVSGKLSLSLTRVSDGRNLLVSQFDSREELIQALICSCFVPLYCGVVPFHSSQYYVDGAVSDNLPRCQLGRTITISPYAGESDLCPRASTLTFHEVRFNNVSFQVNSENLYRVTSTFFPPEAEVRYSCSFPSRLRSEGWLKIHHVTKRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KSY5</accession>
    <name>A0A3B5KSY5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027900451.1">
        <property type="nucleotide sequence ID" value="XM_028044650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KSY5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003218"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003255.1">
        <property type="protein sequence ID" value="ENSXCOP00000003218.1"/>
        <property type="gene ID" value="ENSXCOG00000002535.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114161397"/>
    <dbReference type="KEGG" id="xco:114161397"/>
    <dbReference type="GeneTree" id="ENSGT00940000164977"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003406">
        <property type="term" value="P:retinal pigment epithelium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000142">
        <property type="entry name" value="homeobox protein OTX2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003025">
        <property type="entry name" value="Otx_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013851">
        <property type="entry name" value="Otx_TF_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45793:SF22">
        <property type="entry name" value="CONE-ROD HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45793">
        <property type="entry name" value="HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03529">
        <property type="entry name" value="TF_Otx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01255">
        <property type="entry name" value="OTXHOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="37"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="39"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="91"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="296" mass="31991" checksum="F16C49E706BF7B6A" modified="2018-12-05" version="1">MMSYIKQPHYAVNGLTLSGPGMDLLHTAAVGYPSTPRKQRRERTTFTRAQLDILEALFAKTRYPDIFMREEVALKINLPESRVQVWFKNRRAKCRQQQQQSTGQSKPRPPKKKASPAREAPTEASANPTNGPYSPPPPPPGTAITPSSSSASATVSIWSPASISPLPDPLSASTTPCMQRTTAYPMTYTQAPAYSQSYAGSSSYFTGLDCSSYLSPMHPQLSSTGGALSPITASSMGGPLSQSPASLSSQGYTAASLGFGAVDCLDYKDQAAWKLNFNAADCLDYKDQNSWKFQVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KT83</accession>
    <name>A0A3B5KT83_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KT83"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001732.1">
        <property type="protein sequence ID" value="ENSXCOP00000001707.1"/>
        <property type="gene ID" value="ENSXCOG00000001379.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000289280"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051379">
        <property type="entry name" value="C-type_Lectin_Receptor_IMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46746:SF9">
        <property type="entry name" value="CD209 ANTIGEN-LIKE PROTEIN C-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46746">
        <property type="entry name" value="KILLER CELL LECTIN-LIKE RECEPTOR SUBFAMILY F MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="72" mass="8491" checksum="8BB46DD65553DEA3" modified="2018-12-05" version="1">QSSGRNPATPAKPNPLPSNPKCQLCPDGWLRFQEKCYWFRNDTELNWEESRRFCQDRSADLVVINNPQQQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTM6</accession>
    <name>A0A3B5KTM6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Endoplasmic reticulum chaperone BIP</fullName>
            <ecNumber evidence="3">3.6.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">78 kDa glucose-regulated protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Binding-immunoglobulin protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Endoplasmic reticulum chaperone BiP</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Heat shock protein 70 family protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Heat shock protein family A member 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Immunoglobulin heavy chain-binding protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.10" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027881975.1">
        <property type="nucleotide sequence ID" value="XM_028026174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTM6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003458"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003496.1">
        <property type="protein sequence ID" value="ENSXCOP00000003458.1"/>
        <property type="gene ID" value="ENSXCOG00000002728.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114150012"/>
    <dbReference type="KEGG" id="xco:114150012"/>
    <dbReference type="CTD" id="3309"/>
    <dbReference type="GeneTree" id="ENSGT00940000154787"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd10241">
        <property type="entry name" value="ASKHA_NBD_HSP70_BiP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000008">
        <property type="entry name" value="78 kDa glucose-regulated protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000720">
        <property type="entry name" value="Endoplasmic reticulum chaperone BiP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000153">
        <property type="entry name" value="Endoplasmic reticulum chaperone BiP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000001">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042050">
        <property type="entry name" value="BIP_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 21" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017205262" description="Endoplasmic reticulum chaperone BIP" evidence="21">
        <location>
            <begin position="17"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="631"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="276"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="643"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00069311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="652" mass="72041" checksum="F4D7FEBFAA42116C" modified="2018-12-05" version="1">MKLLWLVVLVVGTVRADDDEKKDSVGTVIGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTSEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWGDSSVQQDIKYLPFKVIEKKSKPHIQVDIGGGQIKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKRDGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSAQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRATMKPVQKVLEDSDLKKPDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGEEDTGEVVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEDIERMVNDAERFADEDKKLKERIDARNELESYAYSLKNQIGDKEKLGGKLSDEDKEAIEKAVEEKIEWMESHQDADLEDFQAKKKELEEVVQPIISKLYGSAGGPPPEGAEEQEKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KUH9</accession>
    <name>A0A3B5KUH9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Fructose-bisphosphate aldolase</fullName>
            <ecNumber evidence="3 7">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class I fructose-bisphosphate aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="3 7"/>
    <dbReference type="RefSeq" id="XP_027901509.1">
        <property type="nucleotide sequence ID" value="XM_028045708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KUH9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002167"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002198.1">
        <property type="protein sequence ID" value="ENSXCOP00000002167.1"/>
        <property type="gene ID" value="ENSXCOG00000001729.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114161974"/>
    <dbReference type="KEGG" id="xco:114161974"/>
    <dbReference type="CTD" id="406496"/>
    <dbReference type="GeneTree" id="ENSGT00950000182987"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00183"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00948">
        <property type="entry name" value="FBP_aldolase_I_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000021">
        <property type="entry name" value="Fructose-bisphosphate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029768">
        <property type="entry name" value="Aldolase_I_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000741">
        <property type="entry name" value="FBA_I"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033379">
        <property type="entry name" value="FrucBisAld_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11627">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00274">
        <property type="entry name" value="Glycolytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00158">
        <property type="entry name" value="ALDOLASE_CLASS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0704">Schiff base</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="363" mass="39652" checksum="B9853C715E145497" modified="2018-12-05" version="1">MPHAYPFLTPEQKKELSDIAQKIVAPGKGILAADESTGSVAKRFQSINAENTEENRRLYRQLLFTADDRIVPCIGGVILFHETMYQKTDDGKTFPQYLKERGMVVGIKVDKGVVPLAGTNGETTTQGLDGLYERCAQYKKDGADFAKWRCVLKITPTTPSKLAILENANVLARYASICQMHGIVPIVEPEILPDGDHDLKRCQYITEKVLAAVYKALSDHHVYLEGTLLKPNMVTSGHSCSHKYSNQEIAMATVTALRRTVPPAVPGITFLSGGQSEEEASVNLNAMNQCPLHRPWALTFSYGRALQASALKAWGGKKENGKACQDEFVKRALANSLACQGKYVSSGTSTAGGESLFVANHAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KW09</accession>
    <name>A0A3B5KW09_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KW09"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004303"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004350.1">
        <property type="protein sequence ID" value="ENSXCOP00000004303.1"/>
        <property type="gene ID" value="ENSXCOG00000003386.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00520000055616"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009801">
        <property type="entry name" value="TMEM126"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16296:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 126A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16296">
        <property type="entry name" value="UNCHARACTERIZED HYPOTHALAMUS PROTEIN HT007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07114">
        <property type="entry name" value="TMEM126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="199" mass="21552" checksum="B32CDF86F55688D7" modified="2018-12-05" version="1">ISLKDFGPGLSRAVIAEMLLKNFEKLSDSDQKLFMYGPLYLGGNAGFAGLIANSLYRRALNVHAARIASNLPMAVLPFLTTTALYNAVVSQPLMSGDLNCPTCSLIRGALVGAVGGGVYPMLLALPLNVGLASRYSTAPMPEKGTMLRYCVEVSRPVFNKMRAVFLLQAFFGTYLGSRHFESYAKLAQITFSKEEGLQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KW97</accession>
    <name>A0A3B5KW97_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Family with sequence similarity 131 member C</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM131 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KW97"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002807"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002844.1">
        <property type="protein sequence ID" value="ENSXCOP00000002807.1"/>
        <property type="gene ID" value="ENSXCOG00000002231.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183106"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026782">
        <property type="entry name" value="FAM131"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15736">
        <property type="entry name" value="PROTEIN FAM131B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15736:SF2">
        <property type="entry name" value="PROTEIN FAM131C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15010">
        <property type="entry name" value="FAM131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="213" mass="22734" checksum="55532A5AB92C53F6" modified="2018-12-05" version="1">HQIIHFAPLLGLVATIKEHITKPTAMAQGRVAHLIEWKGWGGCGEGGGNRAGWSGGWSKGGGGWGAAGTELQEDEQFYSQMTDEIKEARFAAGVAEQFALAEAAMSMWSMSDNVEQPSTSSQAAEGHFLSKYLLDGGSVGVPQHLYSIHTQTYGDSDATSLIHPPLANSTLPPAPGAQRDADRPFEDRSMAMAEAAVRHVDSSSLSEDDVFYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KWV2</accession>
    <name>A0A3B5KWV2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clathrin heavy chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasmic vesicle membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Coated pit</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the clathrin heavy chain family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000002106"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002136.1">
        <property type="protein sequence ID" value="ENSXCOP00000002106.1"/>
        <property type="gene ID" value="ENSXCOG00000001677.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183166"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071439">
        <property type="term" value="C:clathrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045334">
        <property type="term" value="C:clathrin-coated endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000001">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000002">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000007">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000095">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.730:FF:000001">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.110:FF:000001">
        <property type="entry name" value="Clathrin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.110">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055358">
        <property type="entry name" value="CHCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015348">
        <property type="entry name" value="Clathrin_H-chain_linker_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016025">
        <property type="entry name" value="Clathrin_H-chain_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022365">
        <property type="entry name" value="Clathrin_H-chain_propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016341">
        <property type="entry name" value="Clathrin_heavy_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292:SF6">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10292">
        <property type="entry name" value="CLATHRIN HEAVY CHAIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00637">
        <property type="entry name" value="Clathrin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09268">
        <property type="entry name" value="Clathrin-link"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13838">
        <property type="entry name" value="Clathrin_H_link"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01394">
        <property type="entry name" value="Clathrin_propel"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002290">
        <property type="entry name" value="Clathrin_H_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00299">
        <property type="entry name" value="CLH"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50989">
        <property type="entry name" value="Clathrin heavy-chain terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0168">Coated pit</keyword>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clathrin heavy chain linker core motif" evidence="8">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="529"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="678"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="825"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="971"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1120"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1266"/>
            <end position="1412"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="7">
        <location>
            <begin position="1415"/>
            <end position="1558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1616" mass="184914" checksum="D7F2C12D3FB8038B" modified="2018-12-05" version="1">YSLYFPLQNLGVNPANIGFSYLTMESDKFICIREKVGEQNQVVIVDLSDPNNPIRRPISADSAIMNPASKVIALKAAKTLQIFNIEMKSKMKAHTTTEEVMFWKWISVNTVALVTDTAVYHWSMEGDSQPTKVFDRHASLAGCQIIHYRTDEQQKWLLLIGISAQQNRVVGAMQLYSVDRKVSQPIEGHAAAFGEFKVEGNAKPSTLFCFAVRSQAGGKLHIIEVGQPAAGNQPFAKKAVDVFFPPEAQTDFPVAMQIGNKHGVIYLITKYGYIHLYDLESGVCIYMNRISAETIFVTAPHEATSGIIGVNKKGQVLSVCVEEENIVNYATNVLQNPDLALRMAMRSNLVGAEELFGRKFNTLFAQGSYSEAAKVAASAPKGILRTAETIRKFQSVPAQPGQASPLLQYFGILLDQGQLNKFESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKASDPTLALSVYLRANVPNKVIQCFAETGQFQKIVLYAKKVGYTPDWVFLLRNVMRVNPDQGLQFAQMLVQDEEPLANINQIVDVFMEGSLIQQCTSFLLDALKNNRPAEGHLQTRLLEMNLIHAPQVADAILGNQMFTHYDRAHVAQLCEKAGLLQRALEHYTDLYDIKRAVVHTHLLNPEWLVNFFGSLSVDDSLECLRAMLSANIRQNLQLCVQVASKYHEQLGTQALVELFESFKSYEGLFYFLGSIVNFSQEPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLIMVVRGQFSTDELVDEVEKRNRLKLLLPWLESRILEGCEEPATHNALAKIYIDSNNTPERFLKENPFYDSAVVGRYCEKRDPHLACVAYERGQCDLDLIKVCNENSLFKSEARYLVRRKDPELWAKVLEEDNPFRRQLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFKKFDVNTSAIQVLIEHIGNLDRAYEFAERCNEPAVWSQLARAQLHRDLVKEAIDSYIKAVDPSAYMEVVNAASKNNNWEDLVKFLQMARKKARESYVETELIFALAKTNRLAELEEFVSGPNNAHIQQVGDRCYEEGMYEAAKLLFNNVSNFARLASTLVHLGEYQAAVDSARKANSTRTWKEVCFACVDGEEFRLAQICGLHIVIHADELEDLISYYQDRGYFEELIALLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQSHLWAELVFLYDKYEEYDNAVITMMNHATDAWKEGLFKDIIAKVANVELYYKSLSFYLEYKPLLINDLLTILSPRLDHNRAVSFFSKMNQLKLVKPYLRSVQNHNNKAVNEALNNLLTEEEDYQSLRASIDAYDNFDTIDLAQRLEKHELIEFRRIAAYLYKRNNRWRQSVELCKKDKLYKDAMLFAAESKDAELAETLLQWFLEEDRKECFAACLFASYDLLHPDVVLELAWRHNIMDFAMPYFIQVMREYLTKVRAEQRLKTDHLCLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYL5</accession>
    <name>A0A3B5KYL5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KYL5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003162.1">
        <property type="protein sequence ID" value="ENSXCOP00000003125.1"/>
        <property type="gene ID" value="ENSXCOG00000002473.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286977"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001485">
        <property type="entry name" value="Krueppel-related zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001480">
        <property type="entry name" value="Si:cabz01071911.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000557">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002274">
        <property type="entry name" value="Zinc finger protein 432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000839">
        <property type="entry name" value="Zinc finger protein 691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001450">
        <property type="entry name" value="zinc finger protein 774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050826">
        <property type="entry name" value="Krueppel_C2H2_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24377">
        <property type="entry name" value="IP01015P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="85"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="253"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="281"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="309"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="364" mass="41486" checksum="09BF9E0F7314FE05" modified="2018-12-05" version="1">MDQQPRSPTAEVHLDQEEMESVQIKEEHDELQESEQDKESRKTKLKRHRKSDTNYNLSSCKICGKTFTQKYLIAHMRIHTGEKPFKCSTCAKKFILKSHLKNHMKIHSGEKPFSCQTCGKSFIQRMNLTLHMRTHTGEKPFPCEICNKTFASRSQLTTHVRTHTGEKPYACKTCGKSFSTRGTLISHMRSHTGEKPFSCQICGQCFTQKPGLKVHSRKHTGEKPFMCQTCGKSFNQKSALLTHIRTHTGEKPFSCLTCGLCFIMKSSLTQHIRTHTGEKPYSCQICGKRCSLKGNLKFHMRIHTGEKPYSCLTCGKSFAVKCQLTRHMRTHTGEKPFSCQTCGKGFTKNLYLIAHMKTHTGEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZB4</accession>
    <name>A0A3B5KZB4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Muscarinic acetylcholine receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Postsynaptic cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZB4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000904"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000916.1">
        <property type="protein sequence ID" value="ENSXCOP00000000904.1"/>
        <property type="gene ID" value="ENSXCOG00000000566.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160084"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016907">
        <property type="term" value="F:G protein-coupled acetylcholine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007197">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006940">
        <property type="term" value="P:regulation of smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15299">
        <property type="entry name" value="7tmA_mAChR_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000047">
        <property type="entry name" value="Muscarinic acetylcholine receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000534">
        <property type="entry name" value="Muscarinic acetylcholine receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000995">
        <property type="entry name" value="Musac_Ach_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF183">
        <property type="entry name" value="MUSCARINIC ACETYLCHOLINE RECEPTOR M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00243">
        <property type="entry name" value="MUSCARINICR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0770">Synapse</keyword>
    <keyword evidence="11 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="68"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="108"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="188"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="452"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="490"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="88"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="269"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="277"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="548" mass="61441" checksum="0A2B5818BC8473CE" modified="2018-12-05" version="1">MNLTSGSDPAHFFPDKPSGGSFQQWSRYWNLHNISILSRKGGNNSESALNMTFQLPEEEFDPLGGHTVWQVIIIVFLTGSLSFVTVVGNILVLVSFKVNKSLKTVNNYYLLSLAFADLTIGTLSMNLYTTYIIMGQWALGPVVCDLWLAIDYVASNASVMNLLVISFDRYFSVTRPLTYRAKRTTKRAMTMIGLAWSISFILWAPAILFWQYIVGERTVQPNECYIQFLSEPIITFCTAIAAFYLPVSIMAILFWKIYQETEKRAKDIQGLKGSGSGTNPSQAQNLDNGGGKDAVGSQRMSSIMLRQISSQSASSSEICLSSPERNRNNNSTSPGVENQKCCRTLCFQFSAFWTRFPASNRSCNTTTSTVEEAERSSVNGDMAAASEMNKNSQHYESVKVQKGSDGLTPSSSLDQSVAKATRDAVLAKRFATKAKIEINKRKNEKKANEKKAARTLSAILFAFITTWLPYNIMVLVNTFCQDCIPESLWALGYWLCYVNSTVNPMCYALCNKTFRTTFRDILICQWNQNRKKPNFNQRQAVGSRKEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZU4</accession>
    <name>A0A3B5KZU4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Semaphorin 4D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SEMA4D</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZU4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003146"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003183.1">
        <property type="protein sequence ID" value="ENSXCOP00000003146.1"/>
        <property type="gene ID" value="ENSXCOG00000002486.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159594"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043931">
        <property type="term" value="P:ossification involved in bone maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002325">
        <property type="entry name" value="Semaphorin 4D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF18">
        <property type="entry name" value="SEMAPHORIN-4D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017276953" evidence="9">
        <location>
            <begin position="19"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="705"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="11">
        <location>
            <begin position="11"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="502"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="622"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="654"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="791"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="654"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="840" mass="93631" checksum="EC1EAC7D80D422E6" modified="2018-12-05" version="1">MGFGVLSVFLGLLLEVSTHGPHSVPRTSWKLDLMEFSEPGIFNYSTLLLSEERGALYVGARDAIFELNKRNVTVRNQKVQWKVAEHPMEMCTRKGKSRERDCPNYIRVLQIVDDKRLYICGTHAFQPQCDYLEDGRGKCSFDPSQSFTTVMVGDLYSGTAYNFLGSEPIISRYSPSHSLLRTEYSTSWLNPSFVFADVIRAEKHRGDGEDDKVYYFFTEVSVEYEFFGKLLIPRVARVCKGDLGGERTLQKKWTSFLKAKLVCSMPELNFVFNVVHDVFILKGRDWRETVFYGVFTSQGNVGLSAVCAYNMAAVEEVFSKGKYMQKATVEQSHTKWVRYNGIPPSPRPGAQCISNQLRQKNISSSLHLPDKTLQFVRDHPLLEDPVLPIGNGPRLITKDINYTQIVVERVLALDGNIYDVIFTGTKGVLHKSVVFEEDVHIVEEIQLLKNSEPIKNLLLSSTQSVYAGSDSGVIQSPTAFCGKYSTCDDCVLARDPYCAWDPNTAACVCISSVSFKDYQKVLVKPGSSAELPCVVKSNLAHVMWKSNNTLLDEASRFHFIGENGLLIYSVAPEDQGDYQCWSVEWAPAAGKNFSRLLAGYTLKLDLPPRPLARTNRVATTVHNQETSSVLPAEGNGKTERQPLTSASFTSTVLLTTPPQTDPSLTPPSSGTVRLQPSRNHPPNSVIPRPDSQDPAAEYLQHNNSVALLFLFLLFFLLFLAAMVYNCYMQYLPAPCLRLRATLLGSNKCTHQPEYRACEAGLMEASAADKINMTEQPAQNGSQPTKNLQALRDTGYETEPECGNGRLPSHNFGGDSSSQEKPFDVDCDSQSIQFADADDAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZV4</accession>
    <name>A0A3B5KZV4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">CD164 molecule, sialomucin</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD164 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZV4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005679.1">
        <property type="protein sequence ID" value="ENSXCOP00000005613.1"/>
        <property type="gene ID" value="ENSXCOG00000004373.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063929"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007947">
        <property type="entry name" value="CD164_MGC24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11337">
        <property type="entry name" value="MUCIN/PORIMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11337:SF12">
        <property type="entry name" value="SIALOMUCIN CORE PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05283">
        <property type="entry name" value="MGC-24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017301291" evidence="9">
        <location>
            <begin position="29"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="124" mass="13553" checksum="509DC3FECFFD5F18" modified="2018-12-05" version="1">MFGCFVWLFFVCCVLVLVPCPAPPTTLATPTTIHTSVAPIIPTTGNHCLIPELYLVCGVHFALVLLTDVFCFLSPNTTTVAPHKNSTFDAASFIGGIVLVLGLQAVIFFLYKFCKSKDRNYHTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L077</accession>
    <name>A0A3B5L077_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Actin-related protein 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin family. ARP5 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L077"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004202"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004248.1">
        <property type="protein sequence ID" value="ENSXCOP00000004202.1"/>
        <property type="gene ID" value="ENSXCOG00000003300.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00720000108866"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045935">
        <property type="term" value="P:positive regulation of nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033044">
        <property type="term" value="P:regulation of chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051052">
        <property type="term" value="P:regulation of DNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10211">
        <property type="entry name" value="ASKHA_NBD_Arp5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000048">
        <property type="entry name" value="ARP5 actin-related protein 5 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000098">
        <property type="entry name" value="ARP5 actin-related protein 5 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000122">
        <property type="entry name" value="ARP5 actin-related protein 5 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000016">
        <property type="entry name" value="ARP5 actin-related protein 5 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00432">
        <property type="entry name" value="ACTINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0558">Oxidation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="615" mass="69149" checksum="B28AEAA20BD567F8" modified="2018-12-05" version="1">MSPDPVFELPAQCVTPTAAPIVIDNGSFQTRAGWAAPAAEFDSPRLLFRSVAARSRGAARSETQIGNDIPNLEPLRWLLKSQFDRNVVVNFEIQELILDYVFTHLGLSSEGSVEHPILMTEAPCNPLHCRQMMSELLFECYRVPFVSYGVDGLYSFYHNNNQRNQRPNTGIILSSGYHCSHILPVVNGRLDALNSKRVNVAGSQAASYLQRLLQLKYPGHLAAITLSRMEELLHGHSYAAVDYHEELEKWRCPEFYEREVHRIQLPFSGKVPGGCVSAEERQERRAQQLRRLQEINTRRREEKLQQDQERLDRFLAIQELLEDGLLDQFHKSLMELNMDSAEELQSYINKLQLAVEQGRLKLLQSDGGEGKMEVRFISLKCLFPLATVTDNVQNIHKTIQTSQKKCCVVAFYSTVFQPAFNMAEYHQLFVGTERLRCPEILFQPSLIGEDQMGLMETLQYVLARYTPEQQEALVSNVFLTGGNMQHPGMKERVERELLALRPFQSHFKVTMATRPGLDAWYGARDWALEHPAAAGAEGWISRQDYEEKGGEYLSEHCASNVFVPMKLAKPVPVRPAEPPAALQTLTGASAASATVTVAPAPSLSTDSADVNMAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L0D5</accession>
    <name>A0A3B5L0D5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zmp:0000000624</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L0D5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003336"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003374.1">
        <property type="protein sequence ID" value="ENSXCOP00000003336.1"/>
        <property type="gene ID" value="ENSXCOG00000002635.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017838"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019327">
        <property type="entry name" value="WKF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22306">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22306:SF2">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10180">
        <property type="entry name" value="WKF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WKF" evidence="2">
        <location>
            <begin position="83"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="173" mass="19838" checksum="DB921444AA2654A4" modified="2018-12-05" version="1">MNRRTPITSSWKSAVTDRWLINRINTSLNGDPEGPLTPEEKRVLERKLKKIRKKEEKKKLKESGPADPKTTEPGPSASKQALDYLTCWAESRSAWKFQKIRQTWLLQHMYDSEKVPDDTFVVLLQYLEGMFGRAKETTVQKALALVEESGKAPQDASVQQRAQRAREVIQLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L0I0</accession>
    <name>A0A3B5L0I0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L0I0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001337.1">
        <property type="protein sequence ID" value="ENSXCOP00000001314.1"/>
        <property type="gene ID" value="ENSXCOG00000001081.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000204427"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="72" mass="8010" checksum="ED9EB25FF0343D73" modified="2018-12-05" version="1">SGFACLQTPATNIKRTFFLPALFKLLSRVTFIKQRPEVTAYLLVSVIVVGLDCFHKLSQGTFILTEKDGSMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2B0</accession>
    <name>A0A3B5L2B0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Adenylate cyclase activating polypeptide 1b (pituitary) receptor type I</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2B0"/>
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        <property type="gene ID" value="ENSXCOG00000004996.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:peptide hormone binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
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        <property type="term" value="P:cell surface receptor signaling pathway"/>
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    </dbReference>
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        <property type="term" value="P:hypothalamus development"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pituitary adenylate cyclase-activating polypeptide type I receptor"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GPCR_2_extracellular_dom"/>
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    <dbReference type="InterPro" id="IPR002285">
        <property type="entry name" value="GPCR_2_PACAP_1_rcpt"/>
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        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620">
        <property type="entry name" value="PDF RECEPTOR-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45620:SF12">
        <property type="entry name" value="PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE TYPE I RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HRM"/>
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    </dbReference>
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        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
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        <property type="entry name" value="PACAPRECEPTR"/>
    </dbReference>
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        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="263"/>
            <end position="288"/>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
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        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 1" evidence="12">
        <location>
            <begin position="15"/>
            <end position="96"/>
        </location>
    </feature>
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        <location>
            <begin position="109"/>
            <end position="376"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00005314"/>
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            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
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            <dbReference type="Ensembl" id="ENSXCOP00000006448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="440" mass="50930" checksum="91FC275CB7376BB6" modified="2018-12-05" version="1">QQVPSNCVIKREQEKCMEMMALEDSDSEFDCPWFWDNLTCWQPAKVGEVIQMNCPELFSQFMSEEDYELGKVSRNCTEFGWSELFPHYIEACLYEEGNSSHPDMYYVSVKALYTVGYSTSLVSLTTAMVILCRFRKLHCTRNFIHMNLFVSFILRAISVFIKDSVLYAEEDSDHCFIHTLECRAVMIFFHYCVLSNYFWLFIEGLYLFTLLVETFFPEKRYFYWYIVIGWGTPTVCVTIWAVLRLHYDDIGCWDMNDNAAIWWVIKGPVLASIMINFVLFIGIIVILVQKLQSPDIGGNEFAINICNITMAKSLKYWLCCRRLARSTLLLIPLFGIHYTVFAFSPENVSKRERLVFELGLGSFQGFVVAVLYCFLNGEVQSEIKRKWRSWTVNRYFAVDLKHRHPSLASSGVNGGTQLSILSKSSSQIRMSSLQAETPAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L303</accession>
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        <submittedName>
            <fullName evidence="8">F-box protein 30a</fullName>
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    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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    </dbReference>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRAF-type" evidence="6">
        <location>
            <begin position="47"/>
            <end position="89"/>
        </location>
    </feature>
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        <location>
            <begin position="575"/>
            <end position="629"/>
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    </feature>
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        <location>
            <begin position="47"/>
            <end position="89"/>
        </location>
    </feature>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
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            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="710" mass="79678" checksum="DFAC6BB07CE039E8" modified="2018-12-05" version="1">MENLHPHCLKCINRRCMAKPEPGVSCNLVGCPLVCGAVFHSCKLDEHRLLCPYERLPCLNSGFGCPFTIKRIKMAQHLETCPASIVCCTMEWNRWPVSYADHKSYENLSKDFDEVEQLDMALALQDQRMLLESLKVTTNVSKNGEKGAGYGDEMVSVLGSETVEMEEEPYNNLYRASGEATRSLAAALDILTNSNNIGKIIENINGDKNGLTHNGENGESSLVAEGGRNVEMLDSNSDSECELGAVGGVDCAVETDGDDDAANWSEERNFVELHFEKKDVVINQPNQNGDLVWPRIPQDYIPLVAPEPPTHQLVPLTPPFLVSDQVRRSFCRYRCWKQNLEVLSMFTYNVPQALINPYLFRAKMEDKAVDTSDLEVEEDPMGLHGIDLITAALLFCLGDSPGGRGISDSRFVDGYHIDFGTQTFSFPSAILATNTMVGDIASASACDHASPQLSNPSPFHTLRLELVLECVARYQTKQRSMFTFVCGQLFRRDEFSSHFKNVHGDIHAGLNGWMEQRCPLAYYGCTYSQRRFCPSVQGFRIIHDRHLGSFGVQPGLPLKTGDGHSRKPHHIDAQYDQFSSLPFEVLQHIASFLDSFSLCQLSRVSRTMRNVCASLLQMRGMVVLLWEKKRRDDGSPSWQITHKVWRFSTAFGTVNEWKFADIASMADHLKKCKFNTIARREEAIPLPCMCFTRELTKEGRCLRSVLKPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4M0</accession>
    <name>A0A3B5L4M0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AGC-kinase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L4M0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007263"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007356.1">
        <property type="protein sequence ID" value="ENSXCOP00000007263.1"/>
        <property type="gene ID" value="ENSXCOG00000005617.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234842"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="59" mass="6884" checksum="D9E975142FE9F900" modified="2018-12-05" version="1">MKLNPFLHLSSPRDVGNFDKEFTKMAVELTPTDKLFIMNLDQNEFQGFSYTNPEFVIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L681</accession>
    <name>A0A3B5L681_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein phosphatase, Mg2+/Mn2+ dependent, 1Db</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L681"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007823"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007921.1">
        <property type="protein sequence ID" value="ENSXCOP00000007823.1"/>
        <property type="gene ID" value="ENSXCOG00000006003.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155672"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="6">
        <location>
            <begin position="62"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="35"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="461"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="466"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="508"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="536" mass="58863" checksum="2CF5E76280400012" modified="2018-12-05" version="1">MNDSLLFRMSAFSDQGGRKYMEDVIEIRIEYEPTASTDEEYPKSQRHGGQGSAAGPQTVTEPHGLSRPGPATAAWVESLSDEDVCSVGTPASSENVVVDTRRSVAFFAVFDGHGGREAAHFASEHLWDLLKRQRGFWSKEHGEVCAALRKGFIACHQAMWKELPEWPKTITGLPSTSGTTASVIVIRGVHMYVAHVGDSAVVVGVKENNSDITLQALEITQDHKPELPKEKERIERLGGSVMKKSGVNRVVWKRPRLTHNGPVRRSTVIDQIPFLAVARSLGDLWSYDFYSGEFVVSPEPDTTVMTLDPKRHRYIILGSDGLWNMMPPKNAVNMCYSHDKMAGPKGMSCARRLGCTALLFWKERMLRADNTTVIVLALQERGGPPIPMHRDEIVVDMATGIDQIPYPGTPYNTCEIQKPIGPLEAPSPFTESSRTLEQAFGLYEAAFCATTQLLPEADSTLRPADRSTNVFEKQDPSSTQVDCDDSAPPSKKPCRSSSRAPELKDSEQSLQPNNAESGPSEDGGILPQHASALCVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6G7</accession>
    <name>A0A3B5L6G7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CDK2 associated cullin domain 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6G7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007903"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008001.1">
        <property type="protein sequence ID" value="ENSXCOP00000007903.1"/>
        <property type="gene ID" value="ENSXCOG00000006068.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000403"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042652">
        <property type="entry name" value="CACUL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46636">
        <property type="entry name" value="CDK2-ASSOCIATED AND CULLIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46636:SF1">
        <property type="entry name" value="CDK2-ASSOCIATED AND CULLIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin N-terminal" evidence="2">
        <location>
            <begin position="11"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="208" mass="23990" checksum="053B237807C86462" modified="2018-12-05" version="1">MAVEDYRKNHWPNLEEAIDRLLIQNFSDHISVSYAQIYYVYKCVCQQHSELLYSDLTSKISAHLQQVSTQLQASPTENLIENFNIALTQYLASLQCIVPVFIYLNKFYIESKLNRDLKEDLMKLFADHVAEEHLSKLMLLIKAHSMPFQVQPSTMASVVKGLHSLRPWAKLAPALFSSFIPQIHPPAMESQLPDYADCDRKLQMELSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7Z1</accession>
    <name>A0A3B5L7Z1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">alanine transaminase</fullName>
            <ecNumber evidence="8">2.6.1.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-alanine + 2-oxoglutarate = pyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:19453"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.2" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L7Z1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006570"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006650.1">
        <property type="protein sequence ID" value="ENSXCOP00000006570.1"/>
        <property type="gene ID" value="ENSXCOG00000005087.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155265"/>
    <dbReference type="UniPathway" id="UPA00528">
        <property type="reaction ID" value="UER00586"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004021">
        <property type="term" value="F:L-alanine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042853">
        <property type="term" value="P:L-alanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045088">
        <property type="entry name" value="ALAT1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751">
        <property type="entry name" value="ALANINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751:SF469">
        <property type="entry name" value="ALANINE TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="11">
        <location>
            <begin position="16"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="142"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="157"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="178" mass="19690" checksum="3CE395D09EF2FC44" modified="2025-10-08" version="2">MAVCLCPELLSDEHLPLDVRTRALRLLEACDGESVGSYTDSSGLPYIRQTIAKFIMKRDEGVPAYVKNIFISSGAQRALMVIVKLLSGGEGRFQTGVLIPQPCPHTLLPLLDEAGALAMPYRLIEEENWAVNTSELRRALTTSRGLCEPRDKAEKQQAVTSRGNTPKPHQTSRKIIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9G3</accession>
    <name>A0A3B5L9G3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Long-chain-fatty-acid--CoA ligase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a fatty acid(in) = a fatty acid(out)</text>
            <dbReference type="Rhea" id="RHEA:38879"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54536"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58950"/>
            <dbReference type="ChEBI" id="CHEBI:138261"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:54537"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tetracosanoate + ATP + CoA = tetracosanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:33639"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:31014"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:33640"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9G3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006391"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006471.1">
        <property type="protein sequence ID" value="ENSXCOP00000006391.1"/>
        <property type="gene ID" value="ENSXCOG00000004948.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158646"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090434">
        <property type="term" value="F:oleoyl-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044539">
        <property type="term" value="P:long-chain fatty acid import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001579">
        <property type="term" value="P:medium-chain fatty acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000008">
        <property type="entry name" value="Long-chain fatty acid transport protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022272">
        <property type="entry name" value="Lipocalin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43107:SF11">
        <property type="entry name" value="LONG-CHAIN FATTY ACID TRANSPORT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00213">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="14">
        <location>
            <begin position="81"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="585" mass="66179" checksum="6B8B49D2CE3CCA99" modified="2018-12-05" version="1">RMMRLACCTVLLFVLRLLVGLPWYQVLPAILIFYLGSGGWSFLDIVVKTIGRDLHAAYVLLRVKLNVKRHVREKNTLPKIFAETVRRHGDKTALIFEGTGERWTFRQLDEYSNRVANLLLDRGFKEGDVVALFMENRSQYVGLWLGMAKIGVEAALINFNLRLDALVHCVTISDAKAVVFGSELSDAVSEVHSLMRKAVLMFCCGDWDPKRVPQGTECLEPLLTGASSRLPQRPDHRLFYIYTSGTTGMPKAAIVVHSRYYRMAALVYYGFRMTPDDVLYDCLPLYHSAGNIVGVGQCLIHGMTVVIRKKFSASRFWDDCVKYNCTIVQYIGEICRYLLNQPVRDSERQHRVRMALGNGLRQSIWEEFMKRYNIPQIAEFYGATECNCSLGNFGNKVGACGFNSQILPFIYPIRLVRVDEETMELIRGPDGVCIPCKPGEPGQLVGKIIQNDPLRRFDGYVNETATSKKIANSVFKKGDSAYLSGAEGKAGMAAIADPSQSTDLEKLFKDMEKVLPPYARPVFLRFLPEVNKTGTFKFQKTELRHDGFNPTVVSDKLFFLDSSRGRYVQLDEELYRSIVSGKHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9P0</accession>
    <name>A0A3B5L9P0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LDLR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000007205"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007297.1">
        <property type="protein sequence ID" value="ENSXCOP00000007205.1"/>
        <property type="gene ID" value="ENSXCOG00000005567.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156574"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000024">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000001">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000017">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000016">
        <property type="entry name" value="Low-density lipoprotein receptor-related protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050778">
        <property type="entry name" value="Cueball_EGF_LRP_Nidogen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000033">
        <property type="entry name" value="LDLR_classB_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513:SF16">
        <property type="entry name" value="LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46513">
        <property type="entry name" value="VITELLOGENIN RECEPTOR-LIKE PROTEIN-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00058">
        <property type="entry name" value="Ldl_recept_b"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00135">
        <property type="entry name" value="LY"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51120">
        <property type="entry name" value="LDLRB"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="15 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="9 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="1363"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="19">
        <location>
            <begin position="89"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="19">
        <location>
            <begin position="132"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="19">
        <location>
            <begin position="176"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="267"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="19">
        <location>
            <begin position="354"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="19">
        <location>
            <begin position="397"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="19">
        <location>
            <begin position="440"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="19">
        <location>
            <begin position="484"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="573"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="19">
        <location>
            <begin position="656"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="19">
        <location>
            <begin position="699"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="19">
        <location>
            <begin position="748"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="19">
        <location>
            <begin position="791"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="881"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="19">
        <location>
            <begin position="1010"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="19">
        <location>
            <begin position="1053"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="repeat" description="LDL-receptor class B" evidence="19">
        <location>
            <begin position="1098"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="1190"/>
            <end position="1228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1455"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1532"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="1591"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="1254"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="1272"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="1279"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="1291"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="1310"/>
            <end position="1322"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="1317"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="1329"/>
            <end position="1344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
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            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1602" mass="178799" checksum="CAFC6EBDA74BDD5D" modified="2018-12-05" version="1">SIYSSPLLLFANRRDVRLVDGDGVRGESAIVVSELEDAAAVDFLFDESLIFWTDVSEEAIKQTHWNQSFNLVVSGLNSPDGLACDWLGKKLYWTDSETNRIEVANLDGTSRKVLVWMDLDQPRAIALNPGQRYMYWTDWGEEPRIERAGMDGSSRKVIVNKNIHWPNGLTIDLVEEKLYWADAKLSFIHRANLDGSVREAVVEGTLTHPFALTLSKETLYWTDWQTRSIHACNKHSGDKTREILGGIYSPMDIQVLEPYRQPYIQTPCSNDNGGCSHLCLLSPLQPFYSCACPTGVQLKADGKTCKPGAEQVLLLARRTDLRRISLDLPDFTDIVLQVHDIRHAIAIDYDPVEGFVYWTDDEVRAIRRSYIDGSNAMILVTMDINHPDGIAVDWVARNLYWTDTGTDRIEVTRLNGTSRKILISDDLEEPRAIVVDPVNGFMYWTDWGQHPKIERANLDGTARVVLLNSSLGWPNGLAIDYTAGKLYWGDAKTDKIEVINVDGSNRQTLLEDKLPHIFGFSLLGEYIYWTDWQSRSIERIHKATAVREIIIDQLPDLMGLKATKVTETHGTNDCAENNGGCSHLCFWCEQAVRCACPMGLELLSDLRTCVVPEAFLLFTNRDSIRSISLGTNSNDVAIPLTGVKEASALDFDVSERRIYWTDIQAKTISRAYMNGSSVEHVLEFGLDYPEGMAIDWMGRNIYWADTGTNRIEVARLDGQYRQVLVCKDLDNPRLLDNPRSLALDPANGYMYWTEWGGRPRIARAYMDGSTISTLVDKVGRANGLTIDYVEHRLYWTDLDTCMIECTNMQGLQREIIVDDLPHPFGLTQYRDFIYWTDFNLRSIERADKRSGLNRTVVLQGQLELMLDILVFHSSRQDGTNECSQNNGNCGHLCLATPAGAQCRCAFHYTLNPDGKNCSSPSSFLLFSQRASISRMVLGDQSPDIILPIHVTRNVRAVSYDPLERLVYWLDGRQNIRRARDDGTMSSIIVSSPAQQVDNQLHDLSLDPYSRHIYWTCETTNTITVQKMDGRIVGVVVKDDTDKPRAIVVNAEKGYMYFTTVQEHSAKIEGASLDGTERESLFSTGLIRPVALALDNKLGKLFWVDADLKRIESSDLTGANRIVLQDSNILQPMGLTVLGDHLYWIDRQQQMIERVDKLTGEFRTRIQGRISYLTSIHAAWEMDPSEFASHPCSRDNGGCSHICIAKGDGTSTCSCPMHLVLLQDLLHCGEPPTCSTEQFTCSTGEIGCIPMAWRCDGFPECDDGSDEMHCPICSAFQYQCDEGNCIDAQKRCDGEPDCPDKSDEHECDTVCPPNQFRCGDNHCITKKQQCDNYSDCLDGSDELACDYISPSSSFIPSNETTNPIGPVIAIILLMFVMGGACFVCQRVVCRRYKGPNGSFPHEYISGTPHVPLNFISPSSSQHGTYQGISCGKSTLSSVSLMGSSSSGAPLYDRNHVTGASSSSSSSTKGAFYPKILNPPPSPATDRSLYNTEIFYSSNSPSTTRSYRPYHPVHGGAPPTTPCSTDVCDSDYTPHHPPAPGHAAHGKPTKYYMDLNSDSDPYPPPPTPRSQYMSAEESCPPSPSTERSYFHLCPPPPSPCTDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9Y1</accession>
    <name>A0A3B5L9Y1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AGRL2-4 GAIN subdomain A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9Y1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004654"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004711.1">
        <property type="protein sequence ID" value="ENSXCOP00000004654.1"/>
        <property type="gene ID" value="ENSXCOG00000003673.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00960000190587"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032471">
        <property type="entry name" value="AGRL2-4_GAIN_subdom_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16489">
        <property type="entry name" value="GAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AGRL2-4 GAIN subdomain A" evidence="1">
        <location>
            <begin position="23"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="105" mass="11820" checksum="91718B74A487CBF3" modified="2018-12-05" version="1">MAIHELSLKASPGSAPLNPALSVLAPETKPHWELIQHTEGGSAALLRHYEDYANTLAQNMRKTYLSPFTIVACCWPRLQTQSLHPHIKLAANRCSIMQQYIFDKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LA27</accession>
    <name>A0A3B5LA27_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">EMAP like 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Midbody</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat EMAP family.</text>
    </comment>
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        <property type="gene ID" value="ENSXCOG00000003713.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="echinoderm microtubule-associated protein-like 4 isoform X2"/>
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        <property type="entry name" value="Putative echinoderm microtubule-associated protein-like 1"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
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        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
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        <property type="entry name" value="ECHINODERM MICROTUBULE-ASSOCIATED PROTEIN-LIKE 3"/>
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    </dbReference>
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        <property type="entry name" value="WD-40 REPEAT PROTEIN"/>
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        <property type="entry name" value="Beta-prop_EML"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
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            <end position="637"/>
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        <location>
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            <end position="523"/>
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        <location>
            <begin position="644"/>
            <end position="665"/>
        </location>
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        <location>
            <begin position="648"/>
            <end position="659"/>
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    </evidence>
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    <sequence length="665" mass="73948" checksum="D843FB73F027E3FC" modified="2018-12-05" version="1">MFIRGRPITMYVPSNIQNYDNLKMELPSERLELDWVYGYRGRDCRANLFFLPSGEAVYFIACVIVLYHIKNRMQRHYRKHTDSVRCLAVHPDNVRVASGQTAGVDKDGKPMQACVHIWDSTTLVTLQQIGLGTFQRGVGMGSLAFSIADSGTFLCVIDDSNEHILSVWDCTKGTKHAEIKTTNEAVFDVDFNPSDSNNIITCGKSHVYFWTLSAGQFTKKQGIFGKYKKPKFIHCFVFSLTGDVLTGDSEGNILPWVKSAADTKTLGKGAKETYQIVRPIKAHEGSVFTLCILKCGALLSGGGKDRKIIRWSADLAPERECEIPEKFGAVRTVAVVDDEGLLVGTTRNAILRGSFSDGFVAIGHLDEMWGLATHPSQNIFLSCSHDRQVCLWNTEEHNLDWCITLEEYGLCADFCPSGSVVSVGLSTGRWVVLDLVTKEVISEFIDGNEQLSVMRYSPDGRFLAVGSHDNFIYIYSVTESGRRYSRFGRCNGHSSFITHLDWSKDSCYIVSNSGDYEILYWDIAGGCKLLRNRFESKDREWASFTCVLGFHVIGVWLEGSDGTDINALCRSHNERVVAVADDFCKVHLFQYPCPKPKAPSYKYEGHGSHVTNVCFTHNDSHLLSIGGKDTCILQWKMTRGGVGESRETLASASSTSTSSPEPPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LB15</accession>
    <name>A0A3B5LB15_9TELE</name>
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        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="SH3 Domains"/>
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        <property type="entry name" value="Rho_GTPase_activation_prot"/>
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    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
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        <property type="entry name" value="WW_dom"/>
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        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF104">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 27"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00233">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PH domain-like"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="6"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="7">
        <location>
            <begin position="285"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="7">
        <location>
            <begin position="336"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="7">
        <location>
            <begin position="451"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="541"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="8">
        <location>
            <begin position="710"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="68"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="126"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="358"/>
            <end position="383"/>
        </location>
    </feature>
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        <location>
            <begin position="658"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="184"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="658"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="901" mass="103177" checksum="4B1F547A1E906362" modified="2018-12-05" version="1">SLGSVGKMAFVLVEFEFEYKAKDGRRICIKPNERYILVSKTNDHWWHVSKDRDSKPFYIPAEYVKEVTAPPGASSGPKKTESPKSLTKHTKPVTHLPVRDDSKETYRFSTFGLWVNLPSETLKDAPAARNRAHSLDDKNKLMTPNSPRRASVTPEPLTSEDQQLYATPQHGDKKQQGKNTNQDKSIEKSQDSANDKDVDLPPHNIYEMDFPLPPPHFYDMTPEISVTDYDYFPEPPDLAGSSPKESNENLRSGVYVNVAELRKSISESPPSDPSPCSLSYLKQEGWEVHVDEDSGQEYYYHSDTGRTTWDNPFLDSPTDPEQFPPEVPFPPPSPQSDWASEWEQLVDETTGQPYFYNHMSGETSWDPPEQQSPYPSPMEPMSDYPSEDYPGADQAEFYEDIQNTGPPTLPKEYALGHVSRTTIPRANLDRSTPPGWNLTVEPNGTWVFTSENSPDQWIKSVDDQGQTYYYLRDGTKSEWNLPEVKTLLYFADFSGKLKSFYTFHFLFYFPVEHRLLHLIFAQTNLQNNEILAFLIQFQVQNLEKAGILNKTKISENGKKVRKNWVPTWTVLHGGVLTFHKDPKPSPGANKTNQIVPEFTVELKGATIGWATKDKSSKKNVLELKGKNGVEFLIQYDTESIIYDWHKILKDTIQQLVSPYSEQNHQQESPSNAGETDPKKVRTKLMKFLMKRPTLQSVKEKGYIRENVFGCHLHTLCALERTTVPSFVEKCIKAVERRGLDIDGLYRVSGNLAVIQKLRFKADHEELDLEDGQWEDVHVITGALKLFFRELPEPLFPFSHFNDFIAAIRISDYNTKLSHIYELVKSLPTVNHDTMKLLFGHLQRVIEYGEDNRMTVQNVAIVFGPTLLRPETETANITMHMVFQNQIVEFILNEYSRIFHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LB72</accession>
    <name>A0A3B5LB72_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Palmitoyltransferase</fullName>
            <ecNumber evidence="12">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:36684"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LB72"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009443"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009554.1">
        <property type="protein sequence ID" value="ENSXCOP00000009443.1"/>
        <property type="gene ID" value="ENSXCOG00000007194.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156558"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF475">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="13">
        <location>
            <begin position="212"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="387" mass="43974" checksum="D697D5DCA2CAC05A" modified="2018-12-05" version="1">MKWEKLKPPEPDDPMCCCECDIYLYRCCCDCEDLDEAFNRWLKEKPPSYGLQSPVLRTMINQLEISMIPALLLLPLLLKAAALHYLLGITILTALPILVLSYYYATHQRKRRTLFFLTLALYSLAYMYYLFIVEILPRGDVSPLQACVVTTGMILTIVSLIYTKRGPGFVPASHHEANRQRTDAEDDFTHSDGFIQATAAEQTSKYPPTTKWSLCPVCKVMRPPRAGHCRTCGSCVQRLDHHCVWINSCVGQANHRSFLQTLCVFVLTSVYGIGLVLRSLCPHQYLMTALFYCPGVYTQPSTALCFTCCWYSSIITVGLLYLLVVQVLNISFNVTGREAQLALRNKKGQSRLWGLVIDTGEYSRGFYQNWAEFLTMYTSAKKHKAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBB0</accession>
    <name>A0A3B5LBB0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oculocutaneous albinism II</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBB0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008122"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008220.1">
        <property type="protein sequence ID" value="ENSXCOP00000008122.1"/>
        <property type="gene ID" value="ENSXCOG00000006235.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234550"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033162">
        <property type="term" value="C:melanosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042438">
        <property type="term" value="P:melanin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030318">
        <property type="term" value="P:melanocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01116">
        <property type="entry name" value="P_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051475">
        <property type="entry name" value="Diverse_Ion_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43568">
        <property type="entry name" value="P PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43568:SF1">
        <property type="entry name" value="P PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Citrate transporter-like" evidence="6">
        <location>
            <begin position="204"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="668" mass="74781" checksum="13226EB4F3D634FB" modified="2018-12-05" version="1">MTVHEPSFVGVEEPWDNCCMDTERRYRLGSEVAGLSRSNSSEKYETLENLNRSFRLTSSIKYDSPQIYRCLHFSLDGACLSAMNVTDFRDNALLKLQVAGPFAGRAVDSASQEFILIQVEQAEEPGQRRRRTQQVTHNWTIPLHPERSDQKVSTRTFEIDPIVITIQASLQDNDVVPLSMTHQSLYVTKETQVAIAGIILAGVYVLIIFEIVHRTLAAMLGSLAALAALAIIGDRPSLVTVVEWIDYETLALLFGMMVLVAIFSETGFFDYCAVKAYQLSRGRVWPMIIILCLIAAILSAFLDNVTTMMLFTPVTIRLCEVLNLDPRHVLIAEVIFTNIGGAATAVGDPPNVIIVSNQGLRREGIDFASFTGYMFLGICLVLLTSFPFLRMLYWNKKLYNKESIEIVELKHEILVWRQTAQRINPASREETAVKCLLMQKVLNLENLLRKMMKTFQRQISQEDKNWEQNIQELQKKHRITDTVLLVKCASVLAVVIFMFFLNSFVPSIHLELGWIAILGALWLLVLADVQDFEIILHRVEWATLLFFAGLFVLMEVVTMQSLCVTFNKKSAVPEDQRLAVAIILVLWVSALASSLIDNIPFTATMIPVLINLSQDVDVNLPVKPLIFALAMGACLGGNGTLIGASANVVCAGIAEQHGYGFSFVEFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBF5</accession>
    <name>A0A3B5LBF5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chondroitin sulfate proteoglycan 5a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSXCOT00000007938.1">
        <property type="protein sequence ID" value="ENSXCOP00000007840.1"/>
        <property type="gene ID" value="ENSXCOG00000006018.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00410000028737"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048858">
        <property type="term" value="P:cell projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15381:SF1">
        <property type="entry name" value="CHONDROITIN SULFATE PROTEOGLYCAN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15381">
        <property type="entry name" value="CHONDROITIN SULFATE PROTEOGLYCAN 5 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="434" mass="47231" checksum="12F9A03EC7000559" modified="2018-12-05" version="1">MESQIEDQNNFSFRHKLPVIYSSSSPLTNLILLILITTTPDDKVNTTSTVIEEDGPVKVNDVMATVTGMSTSATSAPIGSVTQRAGRMPRGDKDDTDSSGMFPAPTVEKVSVAATPQIIPDSAETDLLLPSNPLREDVVKGEEEGDEEEEGLPHTDPPWIHVKETAMFDLDHLTTTTAPPAAATNPSNQNVLHVDFFDPSSRGRNLNLAPPSPSSLAHELQGAEATSWAMPENYDYLTPYDDGMTTTVDEYSYSTTTDAYETEEDLREAAGSPARSRPRVPGSGSFIPGGSYPGAAPNVLPNGCRVGYQLVNGSCRSACETQPNYCLNGGQCYQLDGEEIFCRYITPLCRNLLISCELPXXXXXXXXXXXXXXXXXXXXXXXXXXKTWSLKGKNCPDINILLENSLKITILSLISIIKETIYWTTCFFLPGQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LC27</accession>
    <name>A0A3B5LC27_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2 DOCK-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LC27"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007261"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007354.1">
        <property type="protein sequence ID" value="ENSXCOP00000007261.1"/>
        <property type="gene ID" value="ENSXCOG00000005612.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154974"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007520">
        <property type="term" value="P:myoblast fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.350:FF:000001">
        <property type="entry name" value="dedicator of cytokinesis protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.350">
        <property type="entry name" value="Dedicator of cytokinesis N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032376">
        <property type="entry name" value="DOCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042455">
        <property type="entry name" value="DOCK_N_sub1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653:SF1">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16172">
        <property type="entry name" value="DOCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 DOCK-type" evidence="4">
        <location>
            <begin position="332"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="441" mass="50782" checksum="9B86547C9780CF45" modified="2018-12-05" version="1">TTLREWATIWRDLYVGDKREMFNSVRDMIYDLIEWRSQILSGTLPQDELTELKQKVTSKIDYGNKYLDLDLVVRDKDGNILDPELTSTVSIFREHEAASKQIEDRIQEEKSQKQNIDLTRQAKFASTPAFALFVTLKNVVCKIGEDAEVLMSLYDPVESKFISENYLVKWSSSGLVKDIDQLHNLRAVFTDLGSEDLKREKISFVCQIVRVGRMELRDNNTKKLTSGLRRPFGVAVMDVTDIITGKMDDEDKQHFIPFQPVAGENDFLQTVINKVIAAKEVNHKGQGLWVTLKLLPGDIHQIRKDFPHLVDRSTAVARKMGFPEIIMPGDIRNDIYVTLVQGDFDKGSKTTPKNVEVTMSVYDEDGKKLENVIFPGAGDEGINEYKSVIYYQVKQPRWFETIKVAIPIEDVNRSHLRFTFRHRSSQDCEYKSQLLKNVFFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LD27</accession>
    <name>A0A3B5LD27_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Calcium load-activated calcium channel</fullName>
            <shortName evidence="15">CLAC channel</shortName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Calcium-selective channel required to prevent calcium stores from overfilling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the TMCO1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027883718.1">
        <property type="nucleotide sequence ID" value="XM_028027917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LD27"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005779"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005845.1">
        <property type="protein sequence ID" value="ENSXCOP00000005779.1"/>
        <property type="gene ID" value="ENSXCOG00000004390.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114151031"/>
    <dbReference type="KEGG" id="xco:114151031"/>
    <dbReference type="CTD" id="54499"/>
    <dbReference type="GeneTree" id="ENSGT00390000002659"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032469">
        <property type="term" value="P:endoplasmic reticulum calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008559">
        <property type="entry name" value="TMCO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20917:SF0">
        <property type="entry name" value="CALCIUM LOAD-ACTIVATED CALCIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20917">
        <property type="entry name" value="PNAS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023322">
        <property type="entry name" value="DUF841_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 15" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="11 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 15 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017262152" description="Calcium load-activated calcium channel" evidence="18">
        <location>
            <begin position="20"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="33"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR023322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="188" mass="21310" checksum="4656A826B049B1DD" modified="2018-12-05" version="1">MSTMFADTILIVFISVCTALLAEGITWVLVYRTEKYKRLKAEVEKQSKKLEKKKETITESAGRQQKKKIERQEEKLKNNNRDLSMVRMKSMFAIGFCFTALMGMFNSIFDGRVVAKLPFVPLSYIQGLSHRNLLGEDYTDCSFIFLYILCTMSIRQNIQKMLGLAPSRAATKQAGGFLGPPPQAAKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEV3</accession>
    <name>A0A3B5LEV3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14 18">Postsynaptic cell membrane</location>
            <topology evidence="14 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LEV3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008216"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008314.1">
        <property type="protein sequence ID" value="ENSXCOP00000008216.1"/>
        <property type="gene ID" value="ENSXCOG00000006304.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022824">
        <property type="term" value="F:transmitter-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13715">
        <property type="entry name" value="PBP2_iGluR_AMPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000001">
        <property type="entry name" value="Glutamate receptor ionotropic, kainate 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000004">
        <property type="entry name" value="Glutamate receptor, ionotropic, AMPA 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000666">
        <property type="entry name" value="Glutamate receptor, ionotropic, AMPA 2a"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutamate receptor, ionotropic, AMPA 4"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
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        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
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        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
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        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
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        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 18" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 18" id="KW-1071">Ligand-gated ion channel</keyword>
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    <keyword evidence="11 18" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="9 18" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6 18" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="510"/>
            <end position="532"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="594"/>
            <end position="616"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="784"/>
            <end position="806"/>
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    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="19">
        <location>
            <begin position="383"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="20">
        <location>
            <begin position="393"/>
            <end position="458"/>
        </location>
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    <feature type="binding site" evidence="15">
        <location>
            <position position="467"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
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        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
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        </ligand>
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    <feature type="binding site" evidence="15">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="644"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="645"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="695"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="16">
        <location>
            <position position="442"/>
        </location>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="16">
        <location>
            <position position="623"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="16">
        <location>
            <position position="650"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="16">
        <location>
            <position position="742"/>
        </location>
    </feature>
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        <location>
            <begin position="51"/>
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        </location>
    </feature>
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        <location>
            <begin position="708"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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            <dbReference type="ARBA" id="ARBA00023257"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU367118"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="852" mass="96342" checksum="2793EDFF1B6C28E2" modified="2018-12-05" version="1">MRSTVQEHSTFRFAVQLYNTNQNVTEKPFHLNYNVDNLESSNSFSVTHAFCSQFSRGVYAIFGFYDRKSMNTLTSFCGALHTSFITPSFPIDADVQFVIQMRPSLRGAILSLLDHYKWERFVYLYDTDRGFAILQAIMESAVANNWQVTARSVGNVNVSTYTQIIREMDNRQEKRFLIDCEVDRIDLILQEVVALGKNTRGYHYIVANLGISNTSLDKVFSGGANITSFQIINPDSPIVQQFLQRWERLDEREFPEAKNTPLKYTSALTHDAILVIAEAFRYLRRQRVDVSRRGSAGDCLANPAVPWSQGIDIERALKMVQVPGMTGNIQFDTFGRRSNYTIDVYEMKPKGPRRIGYWNEYEKFVYIMDQQVTNESSSVENRTIVVTTIMEAPYVMYKKNYMQMDGNDRYEGYCVDLASEIAKHVGIKYKLSIVPDGKYGARDPDTKTWNGMVGELVYGRADIAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHLDENDEAKDPQSPPDPPNDFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLDSGSTKEFFRRSKIAVYEKMWSYMKSAEPTVFVKTTPDGVSRVRKSKGKFAFLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGVATPKSSALRTPVNLAVLKLSEQGILDKLKNKWWYDKGECGTKDSGSKDKTSALSLSNVAGVFYILVGGLGLAMTVALIEFCYKSRQETKKLKLAKNAQNFKPAPPANAQNFATYREGYNVYGTESVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFH9</accession>
    <name>A0A3B5LFH9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Period circadian protein homolog 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Circadian clock protein PERIOD 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000006624"/>
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        <property type="protein sequence ID" value="ENSXCOP00000006624.1"/>
        <property type="gene ID" value="ENSXCOG00000005125.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001222">
        <property type="term" value="F:transcription corepressor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000013">
        <property type="entry name" value="Period circadian protein homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000004">
        <property type="entry name" value="Period circadian protein homolog 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057310">
        <property type="entry name" value="PER1-3_bHLH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048814">
        <property type="entry name" value="Per1-3_PAS-A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022728">
        <property type="entry name" value="Period_circadian-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050760">
        <property type="entry name" value="Period_circadian_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11269">
        <property type="entry name" value="PERIOD CIRCADIAN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11269:SF9">
        <property type="entry name" value="PERIOD CIRCADIAN PROTEIN HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23170">
        <property type="entry name" value="bHLH_PER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21353">
        <property type="entry name" value="Per3-like_PAS-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12114">
        <property type="entry name" value="Period_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PAS" evidence="12">
        <location>
            <begin position="430"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="558"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="625"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="691"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="851"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="933"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1034"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1094"/>
            <end position="1143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1157"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1297"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="79"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="137"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="564"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="869"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="902"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="946"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1094"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="1110"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1161"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1300"/>
            <end position="1315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1339" mass="146264" checksum="4C57E6B037536540" modified="2018-12-05" version="1">MSEDSDPKLYRFSTLDGCEQNREDQGPRRGSMSKLHRMASSYSEGCGRQDRVELQPELGLASEGSDSSHEHQASLGSPRNDRMRQRMPLHEDVEMGGSGSSGSGTESHGNESHGNESHGNESVGSSNGNVKDSALESSVSNKSSNSHSPSPPSSSNAFSLMSSEQDNPSTSGCSSEQSAKAKTQKELFKTLKELKMHLPPEKRSKGKSNTVNTLKYALRCIKQVKANEEYYQMLMINDSQPPGFDVTSYTIEEINTITSEYTLKNTDIFAVAVSLITGKIVYISDQAASILNCKREVFHNAKFVEFLTPQDVSVFYSFTTPYRLPSWSMCTGAESSPTECMQEKSFFCRISGGKEREGDLQYYPFRMTPYLMKVQDAELSEEQFCCLLLAERVHSGYEAPRIPPDKRIFTTTHTPNCVFQDVDESRAVPLLGYLPQDLIGTPILLNMHPSDRPLMLAVHRKILQCAGQPFDHSSIRFCARNGEYITIDTSWSSFVNPWSRKVSFVIGRHKVRMGPVNEDVFAAPAFHGGKMMDSDIQEISEQIHRLLLQPVHNMGSSGYGSYGSNGSHEQQVSISSSSESNGNIPVGNTAEETSKTKPSRTFQEICKGVHMLKNQDSQVWSSAAQRSSGVRVRDSAPHSQVRDNTGANMDDFTYKDQTVCSYQQISCLDSVIRYLESCNVPITVKRKYQFSSNTTSSNSDDDKKGSEDGIQVPQGTHPAPKKPPTGAATVVGGTLAPLTLPSKAESVVSITSQCSYSSTIVHVGDKKPQPESEIIEDVTESPAPPAVPVSMVSPPSQEKEAYKRLGLTKEVLAAHTQKEEQAFLNRCRELRNARSFQKECSSCLHNQRGPANVEVAKQGPTRPETTTKKGNRNRKSKKPRMKHPDSSDSAVSNRKPRPPLQGLNQTSWSPSEASQSAFNVSYPTMVPAYPLYPPTPAAPAQGSRPDASQSSAFGEGQNAQVPPTSTPFPPPIVTPVVALVLPNYLIPHMGQLNQMNQMSQLSYPMQTQLSFTPQQPFPVQTAFSPQHPFQAAQAPYTTQQPFPPPKALTAEPKEGAASRSSTPASGAPEPTTSPPLFESRCSSPLQLNLLSMEEGQRSLERQDSTVAQFGGPGSGAAVGSGATGDKSGALEPRDSGAHSDGNSSSCDLLDILLQEQEDAHSGTGSATSGSMGSGLGSGSASGSSNTSKYFGSIDSLDKSAQGEGEHLIKYVLQEPLWLLMANADDKVMMTYQMPSRDIQRVLREDKERLRQMQKSQPHFTSEQRRELVEEHPWMRRGGLPAAINVKECVYCEDPAQDAAPLEEDLPDMDMGELGEDLGRDTQKGQSQSEETKPQPDSGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGT5</accession>
    <name>A0A3B5LGT5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGT5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008886"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008992.1">
        <property type="protein sequence ID" value="ENSXCOP00000008886.1"/>
        <property type="gene ID" value="ENSXCOG00000006778.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010767"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001164">
        <property type="term" value="F:RNA polymerase I core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038801">
        <property type="entry name" value="TAF1C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049087">
        <property type="entry name" value="TAF1C_beta-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049090">
        <property type="entry name" value="TAF1C_HB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15319">
        <property type="entry name" value="TATA BOX-BINDING PROTEIN ASSOCIATED FACTOR RNA POLYMERASE I SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15319:SF1">
        <property type="entry name" value="TATA BOX-BINDING PROTEIN-ASSOCIATED FACTOR RNA POLYMERASE I SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20641">
        <property type="entry name" value="TAF1C_beta-prop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20642">
        <property type="entry name" value="TAF1C_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TAF1C beta-propeller" evidence="2">
        <location>
            <begin position="180"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="TAF1C helical bundle" evidence="3">
        <location>
            <begin position="423"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="543"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="614"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="634"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="689" mass="76614" checksum="52C4DD9DA7B5BCE6" modified="2018-12-05" version="1">MRLWFQGGTGSLSSWAFTSKRQLGGEVWRQTEPVPVPLLSPRKGEPVLIQKASRVSALLVLFPIVSACPMAYRSRSLDRYCSLLWDVVHDVPPELLGSLLHEELAEQRDRMQFSEAATGGALAFVPFSQGEGCLLYPRGPGMDRLSILLCSFRSKFRPYLRSAMLPEVQLTVSCRPAGCVAVRTDHVCGVWGMREQEEPRLLQVVGTRAAASCVSVSPHILGEVLVASESGAANLWTVGRGMQEVRTEESNLYFNAKSSWRWCEFSAHPRVMLYADRTGVELTDFRSNPCHTLFRISSTSDCQSGERLLLVRFLGDVHPFHHLLTTQHSAYIMDERFPGVPMLKWDHMMEAPPLFCHVAPGSASGTTKVLLGSQSSQEITLLQYSGELAGGSSRLWSAFVFPARLPDQVPAFVAVGNEADWSAPSVLSSATRHAWRRWLQKLRRREGKPGAPPHLAVRTAGLLSVAEPGAAGRHMQGALQQDLQRCMRTRSLLLNRDLDPVPVPPVVDAEAWCDPLSDRLTAAWRGGTEAWLEWWQEERGENRAMKKEALRRRRRREKEAQRAAGSHLRLSGSFTSSISYQTDVDDFSGWSSGTSGPLSDSQRSRPLGRLAAFLEGQDDSPPPDRLDPPSRTPEPQTANQRSCKLADQSLSSLWETQVGMMLPPPGFRRVGRNICSSIYMISFSSNYHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LH42</accession>
    <name>A0A3B5LH42_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NXPE C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NXPE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LH42"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009105.1">
        <property type="protein sequence ID" value="ENSXCOP00000008996.1"/>
        <property type="gene ID" value="ENSXCOG00000006862.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182866"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057106">
        <property type="entry name" value="NXPE4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026845">
        <property type="entry name" value="NXPH/NXPE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16165">
        <property type="entry name" value="NXPE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16165:SF9">
        <property type="entry name" value="NXPE FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06312">
        <property type="entry name" value="Neurexophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24536">
        <property type="entry name" value="NXPE4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017289520" description="NXPE C-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="NXPE C-terminal" evidence="3">
        <location>
            <begin position="298"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="523" mass="58208" checksum="4DF58843D266A264" modified="2018-12-05" version="1">MHFPKTVKAKFIILKIIFFMITNITPTKMAPKPNTITPFGPTEPASVCSFHSASPENATEVNLLKESIAWPETPSVPPDFSLTDTSDPAHSTFTILPRRGGGSWHVGDQLEVLIRISDHRGRPRKSGGDLLYARLQNRAVFAGVAGKVLDHRNGSYTAVFSLLWEGNAQVEVSLVHPSEAITVLAKVTQEYPDRAYFLSVFRSGSVTETVTCNVCLKGPKEKLCNYTDLHTGEPWFCYKPKKLTCDNRITHSKGGFSFKPESNENQLFQRLYLPVYLPDGTSDIVKTKPAGYYYQGAWRALDGTTVRQFNNASARTQCLKGKVVHLYGDSTVRQFFEFLTGNTPGRHVFDLKSPGQAGPYMALDYKNNILLTYRCHGSPILYTPMSAIETRHIATELRTVAGGPDTVIVISVWAHFTSFPIEVYIRRLLNIKRAVVQLLTRAPDTLVIIRTSNPKGSVLSEIRTSSDWYALVNVQLLDAWEMCLAHHLPHSLHPQPPIIKNMIDVVLSHTCPLGGKSEGATKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHD4</accession>
    <name>A0A3B5LHD4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHD4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009091"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009202.1">
        <property type="protein sequence ID" value="ENSXCOP00000009091.1"/>
        <property type="gene ID" value="ENSXCOG00000006935.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161207"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="47" mass="5118" checksum="ED5B00DA3C96168F" modified="2018-12-05" version="1">MGHDPIRAYDFLLKFLLVGDSDVGKGEILESLQDGASESPYGYNLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIV3</accession>
    <name>A0A3B5LIV3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Signal transducing adaptor family member 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIV3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010807"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010932.1">
        <property type="protein sequence ID" value="ENSXCOP00000010807.1"/>
        <property type="gene ID" value="ENSXCOG00000008160.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063841"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039111">
        <property type="entry name" value="STAP1/STAP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16186:SF11">
        <property type="entry name" value="SIGNAL-TRANSDUCING ADAPTOR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16186">
        <property type="entry name" value="SIGNAL-TRANSDUCING ADAPTOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="5">
        <location>
            <begin position="141"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="265"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="337" mass="38101" checksum="2053E8FEF521D23D" modified="2018-12-05" version="1">MAAAPVRQRCGSGGPRSQLPPCYFEGYLEKRGPKEKASRRLWTCLCGNSLYFFNNAKDAHYVEKLDLGGFVSLKDDSSRDRNLEAARLILRMKDGETKFTAPNLEARELWKGFLYSVIDVIISLLHFFTRVFLPISRYYRWARSLRECFRPVSRTEAEVLLERHPDCGNMLLRPGRDGCSLAVTTRQDLNGSVFRHYRVTQRDQGGYVIDVENPIPCATLHDVIDALVEKTAGTLQPFLLEEPYEENITYVSANDENGEKILHTAPSSPLPRVPALPPKQGFPLSRSPAADRRILTSPVPASPTNPMRRLILSPSPLTQTLNEELKLTLEKRKTSQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIW2</accession>
    <name>A0A3B5LIW2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BTB (POZ) domain containing 3b</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Adapter protein for the cul3 E3 ubiquitin-protein ligase complex. Promotes the export of zbtb16/plzf from the nucleus to the cytoplasm and targets zbtb16/plzf for ubiquitination and degradation. Up-regulates neurog1 expression and antagonizes zbtb16/plzf, to promote neurogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with cul3. Interacts (via BTB domain) with zbtb16/plzf.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIW2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009591"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009704.1">
        <property type="protein sequence ID" value="ENSXCOP00000009591.1"/>
        <property type="gene ID" value="ENSXCOG00000007304.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156461"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021987">
        <property type="term" value="P:cerebral cortex development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048813">
        <property type="term" value="P:dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18488">
        <property type="entry name" value="BACK_BTBD3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000003">
        <property type="entry name" value="BTB/POZ domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.820:FF:000001">
        <property type="entry name" value="BTB/POZ domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000015">
        <property type="entry name" value="BTB/POZ domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.820">
        <property type="entry name" value="PHR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049737">
        <property type="entry name" value="Btbd6a-like_BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012983">
        <property type="entry name" value="PHR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038648">
        <property type="entry name" value="PHR_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45774">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45774:SF2">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08005">
        <property type="entry name" value="PHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="7">
        <location>
            <begin position="117"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="519" mass="58114" checksum="8D6CA770B777586E" modified="2018-12-05" version="1">MVDAKGRNMKCLTFFLMLPDSVKSKSSKSSKKGNASGSSKLPPVCYEIITLRSKKKKMSAEIFPTKKPASSTTTTTVQEYQQQNLNNNNTIQNCNWQGLYSTIRERNSVMFNNELMADVHFVVGQPGRTQRLPGHRYVLAVGSSVFHAMFYGELAENKDEIHIPDVEPAAFLAMLKYIYCDEIDLTADTVLATLYAAKKYIVPHLARACVNFLETSLSAKNACVLLSQSCLFEEPELTQRCWEVIDAQAELALRSEGFCDIDSQTLESILQRETLNAKEIVVFEAALSWAEAECQRQEFTSSTDNKRKVLGKAMYLIRIPTMALDDFANGAAQSGVLTLNETNDIFLWYTAAKKPELKFASQPRKGLTPQRCHRFQSCAYRSNQWRYRGRCDSIQFAVDKRVFIAGFGLYGSSCGSAEYSAKIELKRQGVLLGQNLNKYFSDGSSNTFSVWFEYPVQIEPDTFYTASVVLDGNELSYFGQEGMTEVQCGKVTFQFQCSSDSTNGTGVQGGQIPELIFYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJB1</accession>
    <name>A0A3B5LJB1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidylinositol 3-kinase regulatory subunit gamma</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">p55PIK</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of a regulatory subunit PIK3R3 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3K p85 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027867728.1">
        <property type="nucleotide sequence ID" value="XM_028011927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJB1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009701"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009815.1">
        <property type="protein sequence ID" value="ENSXCOP00000009701.1"/>
        <property type="gene ID" value="ENSXCOG00000007379.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114141391"/>
    <dbReference type="GeneTree" id="ENSGT00940000156259"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046935">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09930">
        <property type="entry name" value="SH2_cSH2_p85_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09942">
        <property type="entry name" value="SH2_nSH2_p85_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000006">
        <property type="entry name" value="Phosphatidylinositol 3-kinase regulatory subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000017">
        <property type="entry name" value="Phosphatidylinositol 3-kinase regulatory subunit gamma b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1490:FF:000001">
        <property type="entry name" value="Putative phosphatidylinositol 3-kinase regulatory subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032498">
        <property type="entry name" value="PI3K_P85_iSH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035020">
        <property type="entry name" value="PI3kinase_P85_cSH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035022">
        <property type="entry name" value="PI3kinase_P85_nSH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF10">
        <property type="entry name" value="PI3K21B, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16454">
        <property type="entry name" value="PI3K_P85_iSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00678">
        <property type="entry name" value="PI3KINASEP85"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="10">
        <location>
            <begin position="68"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="10">
        <location>
            <begin position="358"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00057933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00082483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="461" mass="53022" checksum="745D8D97B30E2940" modified="2018-12-05" version="1">MYNTVWTAEKPGEDGDWRDVVMPYSTELIFYLEMDQPPALPPKPIKLQASSSSGVRGDDGDSLQEAEWYWGDISREEVNEKLRDTPDGTFLVRDASTKMQGDYTLTLRKGGNNKLIKIYHRDGKFGFSEPLTFSSVVELIGHYRHQSLAQYNTKLDVRLTYPVSRFQQDQLVKEDNIDAVGKKLLEYHSQYQEKSKEYDRMYEEFTKTSQEIQMKRTAIEAFNETIKIFEEQCQTQERCSQDCVERCSRCSGDKDMERILVNFEKLKSRLGEIHDSKVRLEQDLKTQATENRETDKKMNSLKPDLIQLRKIRDQHLVWLNHKGVRQKRINDWLGIQNDAAAEGGEEEESLPHYDEKSWFVGDVSRTQAEELLGGKPDGAFLIRESSKKGCYACSVVASGAVKHCVIYSTPRGFGFAEPYFLYGSLKELVLHYRLTSLVQHNDALNVRLAHPVHAPAPPLCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJP9</accession>
    <name>A0A3B5LJP9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Neugrin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Neurite outgrowth-associated protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an essential role in mitochondrial ribosome biogenesis. As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mitochondrial ribosomal RNA (16S mt-rRNA), controls 16S mt-rRNA abundance and is required for intra-mitochondrial translation of core subunits of the oxidative phosphorylation system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Forms a regulatory protein-RNA complex, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mt-rRNA. Interacts with 16S mt-rRNA; this interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the neugrin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJP9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008104"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008202.1">
        <property type="protein sequence ID" value="ENSXCOP00000008104.1"/>
        <property type="gene ID" value="ENSXCOG00000006221.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014472"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010487">
        <property type="entry name" value="NGRN/Rrg9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13475">
        <property type="entry name" value="NEUGRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13475:SF4">
        <property type="entry name" value="NEUGRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06413">
        <property type="entry name" value="Neugrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-0221">Differentiation</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0964">Secreted</keyword>
    <keyword evidence="10 18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017423635" description="Neugrin" evidence="18">
        <location>
            <begin position="21"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="41"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="243"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00016593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="293" mass="33093" checksum="897085A02AA60A25" modified="2018-12-05" version="1">MARPFQVCCLLFRFSAPSLASPVYLNGVRFSSRGSSKVWVGQNHVSGDGTRPQTSRFNDDVEPELEGKEKKRQRSVKFDMIRRKMTPPGAPQRKLTWDAIEQIRYLKQEQPEEWTIERLAEGFSVPQDVILRVLKSKFTPSPNRKVKQDAKVMVRLSQQVLPSDSRRQENKLKLPQSQTPVTLIPRQTEGALISETAPAQVLQSKGSASLVPVQPAKLPAGTYKEARVKGSTIDGDGFSTKETEEDEEWEESWDGQVLTEEDLEEILDMDKPAPVVQVGNDFFDAEGNFLYRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJT0</accession>
    <name>A0A3B5LJT0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ty3 transposon capsid-like protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJT0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010905"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011030.1">
        <property type="protein sequence ID" value="ENSXCOP00000010905.1"/>
        <property type="gene ID" value="ENSXCOG00000008239.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183173"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032567">
        <property type="entry name" value="RTL1-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045358">
        <property type="entry name" value="Ty3_capsid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503">
        <property type="entry name" value="LDOC1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503:SF22">
        <property type="entry name" value="TRANSPOSON TY3-I GAG POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19259">
        <property type="entry name" value="Ty3_capsid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ty3 transposon capsid-like protein" evidence="2">
        <location>
            <begin position="87"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="323" mass="35799" checksum="C282754BC61A4CA7" modified="2018-12-05" version="1">MTEHSGQPTDPAEALRRTLADQNSLIQSHDAALRILGNQQSETNQRLDNLTNLLQRVLQQGAAAASAAPDPAPDPVRPPSPEKYSGDVYRCGGFLLQCSLAFNHSPRSFTHDGAKISFIISHLSGRALEWAEARFKDPTNFGCSFQEFLVEFKQTFNQEPDNSSCSRNLLSIKQGPRSVADFAVDFRIGAASSGWNEPALKNMFFHTLNESLKDELATIDEPGSLNDLISLAIRLDNRMRDRHKERIRKNPPVRSPLPVGSPPSYSPPLSQTQSEPEPMQIGRARLSPEERQKRMSADLCLYCGLPGNYIASCPSRSKAQTRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLH4</accession>
    <name>A0A3B5LLH4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SUEL-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLH4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011918.1">
        <property type="protein sequence ID" value="ENSXCOP00000011782.1"/>
        <property type="gene ID" value="ENSXCOG00000008905.1"/>
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        <text evidence="12">Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini.</text>
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        </subcellularLocation>
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        </source>
    </evidence>
    <sequence length="1547" mass="174167" checksum="EAE2DECF40CBA4E3" modified="2018-12-05" version="1">MTDVYLIFLPSAEEQQERKKHKQHKRREAKRPDLQRYQPAAGHGRCHQDSEEGEPGQRDSLDAKAQQSDQSSMSGKRVSEDVLDKKGCTDLSGTKNKDEDRMRGDGSNSLNYKQGTQSLGKIDANSENGKEQQDSLGAPKPAKKVRKPDREFYQPGSRRNVQGKDYGVGRELDKLSPNKQGLTPEPESQLIGGEGNSKKSIPKHRGKDKVTFSDSIEETTEQGRQTVDQTEEKIMSKVERLNIKEKGEVGSPSNNVGDVACTRREKESSAQGGEIKCQEEKTDRKEKGNRRRRGGEKEKKEENETHLESKREEEANQSRKHNQRKLEKEKDKRAPNTDRRKSEKPGEPHHSKQNDNPRENHKIKDNNSNFKDTEKAAKTGSHRDRVVEERDRHRSNLNATTPNSKRYSKSNIRHSRNRTYSSSSASSVTSQDGPRRHMGMEGVKWPRLPGQNNELGDKCEGPRRHSSTESLDRSEVCDREEADRRRRRRKREEWKTSNKSGGRGILRVSLDNQSPVAKDSPVPRGRGRGILVLPARTDISNSPEVGQRLFSTGTRGGAAGRTRGGRGGGVRRLWDPNNPDQKPALTNTQTSKHAALKQPLYLQSGTGYGQLHFLDTDDELSGSPPVAQGDHFQSQQAAAMAYYKFQNSDNPYCYPIATNSPNSTTGQRYPHLYNMGPYQMAHPNGMYPSPSTSPFSGSYRGVGYSQPRVAGGLTFEEDLGRMLKAADAHELQLSNLISRDRVSADGLDRMSQLRVDLLGLYERIILTDIEFSDSQNVDQALWKNVFYQVIERFRQLLKDPSCDNTHDIRNMLLTLLDEGALFFDALLQKLQAVYQFTLEDYMDGMAIRTRPLRKTVKYALISAQRCMICQGDIARYREQATNSANYGKARSWYLKAQQIAPKNGRPYNQLALLAVYTKRKLDAVCYYMRSLAASNPILTAKESLMSLFEEAKRKAEQFERRRRQEQEGGSQGPAVKGRRKWDDGARVEIWIRLSGQTTTPSSQRGGSESSRDSEQDGELGSLTATELNKRFILSFLHAHGKLFTKVGMESFPGVASRVLQEFKTLLQHGPSLLGFTNLLQIITINMFAIYNAHSRGEEGDVRSVLLEQSTALGLDMFALLVQRCTELLKETPAEEGKTEERKGMVRVSAFPLDLRELLPSIKVWSDWMLGHPDEWNPPPCSLECSPDVWQCLADLCNALARVDHEEMLLYKVDTDEGEGDEELTVLQLKEDKLLAGFVPLLAAPQEPCYTDKHTDMAIAADCKRVTVLKYFLEALCGQEEPLLAFKGGKYVSVATCSSPNHSEDTRSRQDSLTEKEEDFEEAGESENDIKELKARRHALANKLAQQQKRRDKIQAVLQTGGQLELEVRPLFLVPDTNCFIDHLDRLKNLLQCGTYIIVVPLIVITELDGLAKGQDGSRGNYNVSVAHMRAVQEQARSAVAFLEKGFEAREPCLRALTSRGNQLESIAFRSEDTSGQQGNNDDVILSCCLHYCKDKAKDFMPPQRNGTVRLQREVVLLTDDRNLRVKALTRNVPVRDIPSFLSWAKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMA5</accession>
    <name>A0A3B5LMA5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UDP-N-acetylglucosamine pyrophosphorylase 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMA5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012097"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012235.1">
        <property type="protein sequence ID" value="ENSXCOP00000012097.1"/>
        <property type="gene ID" value="ENSXCOG00000009144.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153464"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039741">
        <property type="entry name" value="UDP-sugar_pyrophosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952">
        <property type="entry name" value="UDP- GLUCOSE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952:SF4">
        <property type="entry name" value="UDP-N-ACETYLHEXOSAMINE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="266" mass="29963" checksum="F860D1742D962A5D" modified="2018-12-05" version="1">MDPNTSGLVQKLAEAGQSQLLQFWKELSPKDQQELFQDLQGIDFLEINGFFKSTMETSSSTKHEKMDGRMEPVPREVLGSVTRDRESLKDWEVKGLDCIAQSKVAVLLLAGGQGTRLGVSYPKGMYDVGLPSHKTLFQIQAERILKLQQLAEQKTTVKCCVPWYIMTSGRTMESTKDFFSQHKYFGLDKNNVIFFQQGMLPAMDYSGKIILESKGKLSMAPDGNGGLYRALGNQGILDDMQRRGVEFIHVYCVDNILVKVMECQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQI7</accession>
    <name>A0A3B5LQI7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Olfactomedin-like 2Ba</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQI7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013476.1">
        <property type="protein sequence ID" value="ENSXCOP00000013312.1"/>
        <property type="gene ID" value="ENSXCOG00000010092.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157757"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003112">
        <property type="entry name" value="Olfac-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050605">
        <property type="entry name" value="Olfactomedin-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192:SF37">
        <property type="entry name" value="OLFACTOMEDIN-LIKE PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192">
        <property type="entry name" value="OLFACTOMEDIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02191">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00284">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51132">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Olfactomedin-like" evidence="4">
        <location>
            <begin position="48"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="309" mass="36079" checksum="C5E0EEDE6B49B8F7" modified="2018-12-05" version="1">FPSRTFSTIYTATACRKAFLQHDAATIMFHLDNNFSRLPCLWVLFTGECKDTLASIAEPVTHNNYGRPEGAWMKDPKSNDDKIYVTNFHYGNNLLEFHNLEVFKKGRFTNSYKLPYNWIGTGHVVYGGAFYYNRAFSRDIIKYDLRLRYVAAWTMLHDAVFEESSSPWEWRSHSSINFAVDENGLWIIYPALDDEGFLQEVVILSRLNPTDLSMQSETTWRTGLRRNNFGNCFVVCGVLYAVDSYNKRDANLEYAFDTHTNTQMTPRMPFINNYTYTTQIDYNPKEGVLYAWDNRHQVTYNIKFAYVDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRI5</accession>
    <name>A0A3B5LRI5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Protocadherin 20-like</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRI5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012159.1">
        <property type="protein sequence ID" value="ENSXCOP00000012021.1"/>
        <property type="gene ID" value="ENSXCOG00000009097.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167495"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000020">
        <property type="entry name" value="Dachsous cadherin-related 1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000033">
        <property type="entry name" value="FAT atypical cadherin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000016">
        <property type="entry name" value="Protocadherin 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF276">
        <property type="entry name" value="PROTOCADHERIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Cadherin" evidence="12">
        <location>
            <begin position="25"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="12">
        <location>
            <begin position="96"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="12">
        <location>
            <begin position="205"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="12">
        <location>
            <begin position="421"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="12">
        <location>
            <begin position="525"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="12">
        <location>
            <begin position="640"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="735" mass="80946" checksum="E044AA8749E48530" modified="2018-12-05" version="1">HFSIKEELEPHTLVGSLNKHFAPPYQLLTEEYLWMNKSTGNFYITEKKMDREHLCSEETKDDECIILQSVIVGPSRELEQFSVIIEDINDNAPYFENSKICLDISEDVPAGTSLMLDDQARDRDSGPNEQLKYELKGSGGVFSLKVEEEGPLIKLIVQKALDRETQDLYQMQLVATDCGVQPLSVSVPIFVTVTDVNDNCPSFSPNSPLSATISGDSKKDTVVTQVVAADPDLGPNAAIVYSLSPKVSERAKKLLILSSHSGHIRLTQDLEIDSSEELVLKVLASGLHCPPAETQVTVSLLPKATQGLTIKIGFIAKHYNQTVVIPESQPPTPLAVLELEGDSSFKDSSLAIEGDVPFFLSPQSGKYLLSTSKPLDYEMKSEYHISVVVKGSSSERSVIAPPQREIRVMVEDVNDNAPHFSQTDYQLEVEENNEPAVILLQVSATDADSGLNGRVTYRLDKHTSAIFKVDSVTGQLSALVPLDREQKSVHKLTVFARDSGVPPLESQASVIIRVLDRNDNAPAFSTPHFIFFIPENAPPFAQVGLIEVEDPDEGENGKIELQIVQGNTSFVVDSIQRMLHTTTNLDREMTDRHELYLAVSDNGRPVALTSSARVTIFIEDINDNNPKVILPSSNYSCLVVSPGTPVGTNVTKVYAIDEDSGVNSEITYSVVAPKSVWQNTPFQVDSKSGNITLRQELLQENMGMHNLFIVVRDNGKPTPLYTTVWINLLVNKSTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRK7</accession>
    <name>A0A3B5LRK7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027863890.1">
        <property type="nucleotide sequence ID" value="XM_028008089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRK7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012041"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012179.1">
        <property type="protein sequence ID" value="ENSXCOP00000012041.1"/>
        <property type="gene ID" value="ENSXCOG00000009110.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114138700"/>
    <dbReference type="KEGG" id="xco:114138700"/>
    <dbReference type="GeneTree" id="ENSGT01150000286930"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000002">
        <property type="entry name" value="Gap junction protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034634">
        <property type="entry name" value="Connexin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF49">
        <property type="entry name" value="GAP JUNCTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118220">
        <property type="entry name" value="Connexin43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0303">Gap junction</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="13">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="14">
        <location>
            <begin position="160"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="306"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="330"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="361" mass="41189" checksum="188D9B36AFFAF9CF" modified="2018-12-05" version="1">MGDWNLLGKLLEKAQEHSTVVGKVWLTVLFIFRILVLSAATEKVWGDEQSGFTCDTKQPGCENVCYDITFPISHVRFWVLQIIFVSTPTLIYLGHILHLVRMEEKQKEKDKERQQQVDKQAPFVDTHHKKALKRDEKGRVRLQGKLLRTYVFNVIFKTLFEVGFIVAQYLLYGFELKPMYTCDRPPCPNVVNCYISRPTEKTIFIIFMLGVASVSLFLNLIEIYHLGFTKCRQGITFKRGQQNLQSISKGHRETPVPLVPSYDDYFREHSQVQPSYPPVPSYNLSPLSEGTDSSFHPYHSKAAYKQNKDNLAVERSSSKPEECDVKGKNGSVSAPGSPTQTRSSHGGKHSSSRTRIDDLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRV2</accession>
    <name>A0A3B5LRV2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alpha-actinin-2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRV2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013530"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013697.1">
        <property type="protein sequence ID" value="ENSXCOP00000013530.1"/>
        <property type="gene ID" value="ENSXCOG00000010254.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000179259"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="73" mass="8825" checksum="65A37BE4913C698E" modified="2018-12-05" version="1">MIHGTTVHYDNGYEDEYMLQEEEWDRDMLLDPAWEQQQRKVCLKFDVLIKCQLFIVMKLYQCNSLRGQLRPGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LS92</accession>
    <name>A0A3B5LS92_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">TM2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TM2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LS92"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010684"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010808.1">
        <property type="protein sequence ID" value="ENSXCOP00000010684.1"/>
        <property type="gene ID" value="ENSXCOG00000008070.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157668"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007829">
        <property type="entry name" value="TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050932">
        <property type="entry name" value="TM2D1-3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21016">
        <property type="entry name" value="BETA-AMYLOID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21016:SF1">
        <property type="entry name" value="TM2 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05154">
        <property type="entry name" value="TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="TM2" evidence="9">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="176"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="337" mass="36411" checksum="24630D1FE608E235" modified="2018-12-05" version="1">MAPRPQDPEGNTPSRDGWLIMAAPFGRLLLLTLLLFCCGSGSGLKATEAEEAEDCRHLRLGQYPSIDESTQEPENCRHGVAFVDCLPAPNVTCRLSNGTEVRFSGDEVGFNRTVACRNVTGYSYKVAVALSLFLGWLGADRFYLGYPALDLWSLGRIGRGHLASADPRLPVPTFQTEPASACGGAAVRSRPRVRGGGAAQRPGPWADSTPGPLGSGPATQSRRAAHLRPAARPAGLRRGGPEAARLLHQRRPAADHQSHRRGDRGETRPGSGPEHGELPRHTRGTNSRAVPVQVRHRPSRVRGQRSGLEQMDPAGFPNRSGFSVNASSKLFHLFIYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSA7</accession>
    <name>A0A3B5LSA7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EF-hand and coiled-coil domain containing 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSA7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012427.1">
        <property type="protein sequence ID" value="ENSXCOP00000012281.1"/>
        <property type="gene ID" value="ENSXCOG00000009287.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165705"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031601">
        <property type="entry name" value="CCD48"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15799">
        <property type="entry name" value="CCD48"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="370"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="283"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="504" mass="55810" checksum="D2A67CD1EA79E9E7" modified="2018-12-05" version="1">MRRTALAPPRVQPSPRAARKSEWLRSALAHHHCPDPAAENEIVVLATGIDQYLQEVFHHLAHPSRDDTVSGEDFAALCVVLGLPGAQRTPEAEDGRGGGGAEQLEQRAEFADVCSGLPERLSFRDFHSRLCGCFRVRTAPGVSGGRAWRLPVSQDTELVERQIRLRWPRVRRRKCVSFDLGRDQTGTRSRTVRSGAEEELREQGTLTVGALRELVEDLRSALQGSDARCLALEVALRRERSRTPAGPCSQGSDGTRKGQRRWDIRDPLLRELKLIRSSRDGQLEEAIKFNERLEEELQWAYQEVRKLQGSESSLRRENSQIRRRAEEAREALNVGLQKVRLIQEQARLVPQLQSKITQLETELLRYSCGHGTPEGFTPAHSPPDAEGLQRAVEGRAASDEEEDDGSLEETGNCCSSKEKSVASRSPGCGEGSVTVLLCYYPLVPSRHMEGKTGSSVHHSYQSIQITDYNLSASSVCSLILITESDSLDFPLECVTHGPGENGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSN5</accession>
    <name>A0A3B5LSN5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mediator of RNA polymerase II transcription subunit 17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mediator complex subunit 17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MED17</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6 7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the Mediator complex subunit 17 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSN5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012401"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012552.1">
        <property type="protein sequence ID" value="ENSXCOP00000012401.1"/>
        <property type="gene ID" value="ENSXCOG00000009368.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011810"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019313">
        <property type="entry name" value="Mediator_Med17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13114">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13114:SF7">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10156">
        <property type="entry name" value="Med17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="45"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="639" mass="71700" checksum="DF4F3438698800EC" modified="2018-12-05" version="1">MSGGPAVRISIESSCEKQVQEVDFHGTETYVPPLSMSQNLTKLAQRIDFSQGSDSEEDGADGEPRDREWGKQETEEEEGTVKFQPSLWPWDSVRNNLRSALTEMCVLHDVLSVVKEKKYMALDPVSQDPNTPQVFQLISKKKSLATAAQLLLKGAEKLSKSVAENQENRRQRDFNAELLRLRSQWKLRKVGDKILGDLSYRSAGSLFPHPGTFEVIKNTDIDLDKKIPEDYCPLDVQIPSDLEGSAYIKVSIQKQAPDIGDLGTVNLFRRPQKSKAGPPWHLKLEAAQNVLLCKEIFAQLSREAVQIKAQIPHIVVKNQIISQPFPGLQLSISLCHSAGERKSSRASPEKPKPDDHLYVLEHNLHQMMREFHKQQLSSVVMPHPASAPFGHKRLRLAGPMAYDKAEISSLQQGEGVLEKIIKQAKHIFLRSRTARTIDSLASRIEDPQIQAHWSNINDVYESSVKVLITSHGYEQICKSIQLQLNIGVEQIRVVHRDGRVVTLSHQEQELQDFLLSQMSQHQVHAVQQLAKVMGWHVLSFSNHVGLGPVESIGNASAITVASLNGEYAISVRNGPESGCKVLVQFPRSQIKELPKSDAVQDAKWSQLRGPHREVNWSKMEGRNFVYKMELLMAALTPCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSN8</accession>
    <name>A0A3B5LSN8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSN8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012406"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012557.1">
        <property type="protein sequence ID" value="ENSXCOP00000012406.1"/>
        <property type="gene ID" value="ENSXCOG00000009376.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153649"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18668">
        <property type="entry name" value="CD1_tandem_CHD5-9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051493">
        <property type="entry name" value="CHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46850">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46850:SF1">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="3">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="3">
        <location>
            <begin position="60"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="245" mass="29019" checksum="6040E54BAE185F3E" modified="2018-12-05" version="1">YTNAEEFFVKYKNYSYLHCEWATMEQLEKDKRIHQKIKRFKTKHAQMRTRSLLTQTTSVTGHLKLIFSDLLQPVIYYLVKWCSLPYEDATWELKEDVDEGKVEEFGKIQNRQPRLKRSARPPASSWKKLEETREYKNGNTLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSITLLSEVYAAGVQGPFLVIAPLSTITNWEREFSTWTDMNAIVYHGSLASRQMIQQYEMYCKDDKVKNAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LT10</accession>
    <name>A0A3B5LT10_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sorting nexin-27</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the retrograde transport from endosome to plasma membrane, a trafficking pathway that promotes the recycling of internalized transmembrane proteins. Following internalization, endocytosed transmembrane proteins are delivered to early endosomes and recycled to the plasma membrane instead of being degraded in lysosomes. SNX27 specifically binds and directs sorting of a subset of transmembrane proteins containing a PDZ-binding motif at the C-terminus: following interaction with target transmembrane proteins, associates with the retromer complex, preventing entry into the lysosomal pathway, and promotes retromer-tubule based plasma membrane recycling. SNX27 also binds with the WASH complex. Interacts with membranes containing phosphatidylinositol-3-phosphate (PtdIns(3P)). May participate in establishment of natural killer cell polarity. Recruits CYTIP to early endosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LT10"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012516"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012667.1">
        <property type="protein sequence ID" value="ENSXCOP00000012516.1"/>
        <property type="gene ID" value="ENSXCOG00000009461.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183212"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13338">
        <property type="entry name" value="FERM-like_C_SNX27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01777">
        <property type="entry name" value="FERM_F1_SNX27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23070">
        <property type="entry name" value="PDZ_SNX27-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06886">
        <property type="entry name" value="PX_SNX27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.60:FF:000002">
        <property type="entry name" value="sorting nexin-27 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000061">
        <property type="entry name" value="sorting nexin-27 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000075">
        <property type="entry name" value="sorting nexin-27 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000003">
        <property type="entry name" value="sorting nexin-27 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037827">
        <property type="entry name" value="SNX27_FERM-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037833">
        <property type="entry name" value="SNX27_PX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037835">
        <property type="entry name" value="SNX27_RA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431">
        <property type="entry name" value="SORTING NEXIN 17 AND 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12431:SF17">
        <property type="entry name" value="SORTING NEXIN 27B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PDZ" evidence="13">
        <location>
            <begin position="48"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="14">
        <location>
            <begin position="166"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="15">
        <location>
            <begin position="278"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="28"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="545" mass="61698" checksum="233BE22B70ED7E24" modified="2018-12-05" version="1">MLDEDGVCSSVPPLPRPSSRNGSGSGSVTPDTGGSWQTPTTVTSGPRLVRIVKSDSGYGFNVRGQVSEGGQLRSINGELYAPLQHVSAVLPGGAADRAGISKGDRILEVNGVNVEGATHKQVVDLIRAGEKELVLAVLSVPPQEADCLDPGDDVSALSCYDYSDKQAVPISIPSYKHTELNSEKFVVYNVYMAGRQLCSKRYREFVILHQNLKREFANFTFPKLPGKWPFSLSEQQLDARRRGLEEYLEKVCSVRVIGESDIMQEFLSESDENYNGVSDVELRIAMPDNTTLTVRVRKNSTTDQVYQAVVMKLGIDGITASYFALFEVINHTFVRKLAPNEFPHKLYVQNYTSAIPGTCLTLRKWLFTTEEEILLNDNQLAVSYFFHQAADDVKKGFIKVEQKSYQLQKLAEQKKMSMYLSLLRSCEGYNEIVFPHCSCDSRRKGHVITAISIHNFKLHACTEEGSLESQVIAFEWGEMQRWDTDEEGMAFCFEYARGEKKPRWVKIFTPYFNYMHECFERVFCELKWRKENIFQLLRHRRDGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTE5</accession>
    <name>A0A3B5LTE5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Interleukin-17 receptor D</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTE5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012646"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012798.1">
        <property type="protein sequence ID" value="ENSXCOP00000012646.1"/>
        <property type="gene ID" value="ENSXCOG00000009546.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156669"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030368">
        <property type="term" value="F:interleukin-17 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11530:FF:000003">
        <property type="entry name" value="Interleukin-17 receptor D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039465">
        <property type="entry name" value="IL-17_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031951">
        <property type="entry name" value="IL17R_D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013568">
        <property type="entry name" value="SEFIR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15583">
        <property type="entry name" value="INTERLEUKIN-17 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15583:SF14">
        <property type="entry name" value="INTERLEUKIN-17 RECEPTOR D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16742">
        <property type="entry name" value="IL17R_D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08357">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51534">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="SEFIR" evidence="10">
        <location>
            <begin position="347"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="562"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="640"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="434"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="574"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="654"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="683"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="734"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="782" mass="86580" checksum="F913DB25693B3046" modified="2018-12-05" version="1">MCSSQLSDFGFKTTTLQKHKILLSIFVLVSNKTNLQARCTMLRYPFVLFFSFVSDCSLSYPLGKHVIHEVSNVSFSHLACDDQAAVVVHWSASPLGIEHIKGFRVYLEDKNPDGKQCQHLILKDPRQLNYSFRSTKLSSQPFSSLTFDTDYMVRVVPFPSLMNESFFPPSFLRTNSCEILLGSDNLVCKPFWKPKTLNVSQLGSNLHVTFDLAPSSFNFQLYYLYYRLRQDGLVRVQRCRPDLNQARTTCVLQDVTPGTYVIELRDNSNTTRRQMQFHVSQVHSPWAGPIRAMAITVPLVIMSAFATLFTVVCRKKQQENIYSQLDEESSESSNQSAALNSERPWPRPKVFICYSNRDCPKHTSVIQSFAYFLQDFCGCEVVLDLWEHLEMCKEGQMSWLSRQLDEANFIITVCSKGLRYYMEKKSRRGKTPIGRRSNSSSGSPTGGSHGDLFVVAVAMIAEKLRLVKQNADSGDPELSRFMAVYFDYSTESDIPTALSLAPRLKLMDQLPQLYGQLHSSRSALADPEQQPDNISRRNYFRSKSGRSLYVSICNMHQHISQNPDWFEKQPSPPGASSAPSDSSSSKHAPLPPQPTCSASVPQPEQRFDSGVVLNEVLVRTPSMEGLEEEARRNVLLLAAGSSPEPPHCSASIPGSTSRLSTGTSGVSSEESSSSSSAPCVLQDEERPAPGHTEEPGSSGPDAPPPRDSGIYDSSVPSSELSIPLMEGLSHDQADSSSLADSESSSSGLGDEEPPAVSSLRCSAACKPQLHQLERSDAHAASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTE6</accession>
    <name>A0A3B5LTE6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTE6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011084"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011211.1">
        <property type="protein sequence ID" value="ENSXCOP00000011084.1"/>
        <property type="gene ID" value="ENSXCOG00000008373.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244843"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903037">
        <property type="term" value="P:regulation of leukocyte cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050863">
        <property type="term" value="P:regulation of T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000142">
        <property type="entry name" value="V-set domain-containing T-cell activation inhibitor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050504">
        <property type="entry name" value="IgSF_BTN/MOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100">
        <property type="entry name" value="BUTYROPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24100:SF151">
        <property type="entry name" value="ICOS LIGAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="29"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="219" mass="24877" checksum="17DFA2E917A62707" modified="2018-12-05" version="1">SRSFTSQTTPSSLSSFRLAIILKALFIFPGEYQLEKPIKAQVGSDVILPCLVKPPVNVRDAAVEWMFDSSKTVHLFRNEADDRESQDDRYKGRTYLNHTMLELGNISLKLSNVTKNDEGIYSCFVHRLPDLSEEKHVTLIVVNSEWLHVNSVQNLLRTFHPDHTALNDLIHSRSQRTTTLNYSAISYAVPPGLTAWHRSTFVFPFGSHPNKTLRVQPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LU04</accession>
    <name>A0A3B5LU04_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="1">Efficient DNA binding requires dimerization with another bHLH protein.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027886021.1">
        <property type="nucleotide sequence ID" value="XM_028030220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LU04"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011299"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011430.1">
        <property type="protein sequence ID" value="ENSXCOP00000011299.1"/>
        <property type="gene ID" value="ENSXCOG00000008535.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114152352"/>
    <dbReference type="KEGG" id="xco:114152352"/>
    <dbReference type="GeneTree" id="ENSGT00940000160871"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000006">
        <property type="entry name" value="Neurogenic differentiation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022575">
        <property type="entry name" value="NeuroD_DUF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF83">
        <property type="entry name" value="NEUROGENIC DIFFERENTIATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12533">
        <property type="entry name" value="Neuro_bHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="10">
        <location>
            <begin position="104"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="15"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="259"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="27"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="358" mass="39821" checksum="0A937B4AC63535C8" modified="2018-12-05" version="1">MLSRLFSEVLPDVQRLAADWVQDSDSDGCKVKEEREGCHLGEDDLDEPREGSSRAESEMAEDDDDEDDDGEEEECGDENGGDKPKKRGPKKRKMTAARVERSKLRRVKANARERTRMHDLNSALDNLRKVVPCYSKTQKLSKIETLRLAKNYILALGEILRNGKRPDVVAYVQMLCKGLSQPTTNLVAGCLQLNTRNFLTEQCADGARFHMPSSPFSVHPYPYRCSRLSSPHYQPGAGALNVRGPSYSGSGYEAVYMPGGTSPDYNSPEYEGQHSPPVCLNGGLSGKQQESSDTDRSYHYSMHYSGLTTSRPNHGLHFGPSGARSGGAHSENIPPFHDVHLHHDRAPPYEDLNAFFHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LW84</accession>
    <name>A0A3B5LW84_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calpain catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LW84"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013576"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013743.1">
        <property type="protein sequence ID" value="ENSXCOP00000013576.1"/>
        <property type="gene ID" value="ENSXCOG00000010278.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154784"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000001">
        <property type="entry name" value="Calpain-1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF395">
        <property type="entry name" value="CALPAIN 2, (M_II) LARGE SUBUNIT A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="7">
        <location>
            <begin position="45"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="377" mass="41519" checksum="28F3378615CF5281" modified="2018-12-05" version="1">MSGVASTLAKKRALAAGFGTNSNAIRYLNQNFEALRAQCLSSGKLFCDPTFPAEPESLGFNELGRNSSKTRGVTWKRPTELVSKPEFIVGGATRTDICQGALGDCWLLAAIASLTMNEFVMARVVPTDQGFGDNYAGIFHFQFWQFGEWVDVVIDDRLPVKDGQLLFVHSAEGREFWSALLEKAYAKANGCYEALSGGSTTEGFEDFTGGIAENYDLNRPPSNLFQIIKKALEAGALLGCSIDVSSLAADSEAVTRQKLVKGHAYSLTGAVEVNYRGRQEKLVRMRNPWGQVEWTGAWSDGSSEWSHVQGDCPHANSEDGEFWMSFSDFQRHYSRIEVCTLTPDTIEDDSVKHWSVSKFDGTWRRGSTAGGCRNHPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWD6</accession>
    <name>A0A3B5LWD6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Dual specificity mitogen-activated protein kinase kinase 7</fullName>
            <ecNumber evidence="20">2.7.12.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">JNK-activating kinase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">MAPK/ERK kinase 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">c-Jun N-terminal kinase kinase 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.2" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWD6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016273"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016479.1">
        <property type="protein sequence ID" value="ENSXCOP00000016273.1"/>
        <property type="gene ID" value="ENSXCOG00000012298.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158914"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001756">
        <property type="term" value="P:somitogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000040">
        <property type="entry name" value="Dual specificity mitogen-activated protein kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000214">
        <property type="entry name" value="Dual specificity mitogen-activated protein kinase kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052468">
        <property type="entry name" value="Dual_spec_MAPK_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47238:SF2">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE HEMIPTEROUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47238">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16 28" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="15" id="KW-0346">Stress response</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="29">
        <location>
            <begin position="86"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="28">
        <location>
            <begin position="3"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00038999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00051693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00073834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00077430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00081151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00083185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="408" mass="46489" checksum="40FD2A6B4E90C720" modified="2018-12-05" version="1">LAMSSLEQRLSRIEEKLKQENEEARRRIDLNIDMSPQRPRPRPIIVIQLSPAPAPSQRAGIIQSIAPSATAPRHICAPRFCSHPLFPHRSIYSISSFIEIKPFIIDERLGYLLIQHVLVKMILYVLCQQQMRRTGNKDENKRILMDLDVVLKSHDCPYIIQCYGAIVTNTDVFIAMELMGTCAEKLKKRIQGPIPERILGKMTVAIVKALLYLKEKHGVIHRDVKPSNILIDAKGQIKLCDFGISGRLVDSKAKTRSAGCAAYMAPERIDPPDPTKPDYDIRADVWSLGISLVELATGQFPYKNCKTDFEVLTKVLQEDPPVLPLSMGFSLDFQSFVKDWNHNRSLLKNNWSLSCFSSLPQEHSFIRRYEVLEVDVAGWFQAVMERTESPRSSQCYSHQHMLHSLLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWP0</accession>
    <name>A0A3B5LWP0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fibroblast growth factor</fullName>
            <shortName evidence="3">FGF</shortName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027864815.1">
        <property type="nucleotide sequence ID" value="XM_028009014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWP0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015552"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015745.1">
        <property type="protein sequence ID" value="ENSXCOP00000015552.1"/>
        <property type="gene ID" value="ENSXCOG00000011759.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114139226"/>
    <dbReference type="KEGG" id="xco:114139226"/>
    <dbReference type="CTD" id="564770"/>
    <dbReference type="GeneTree" id="ENSGT00940000158380"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048703">
        <property type="term" value="P:embryonic viscerocranium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000004">
        <property type="entry name" value="Fibroblast growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00263">
        <property type="entry name" value="HBGFFGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0339">Growth factor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="211" mass="23575" checksum="29E48BC493309125" modified="2018-12-05" version="1">MAAATLGEVGGVLHGIDGVVHVGSHFVLTPPGYGPTLLGEPHLAPGDRRYLPTTPDLKHLKGILRRRQLYCRTGFHLEILPDGTVQGTRKDHSRHGILEFISLAVGLISIKGVDSGLYLGMNDKGELYGSEKLTAECVFREQFEENWYNTYSSNLYKHGDKGTRYFVALNKDGTPRDGTKSRRHQKFTHFLPRPVDPERVPELYKDILGHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWX3</accession>
    <name>A0A3B5LWX3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA mismatch repair protein MSH3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4 11">DNA mismatch repair protein MSH3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">MSH3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the post-replicative DNA mismatch repair system (MMR).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA mismatch repair MutS family. MSH3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWX3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012334"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012483.1">
        <property type="protein sequence ID" value="ENSXCOP00000012334.1"/>
        <property type="gene ID" value="ENSXCOG00000009317.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074949"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016447">
        <property type="term" value="P:somatic recombination of immunoglobulin gene segments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.10:FF:000004">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000004">
        <property type="entry name" value="DNA mismatch repair protein Msh3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF122">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="14">
        <location>
            <begin position="763"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="64"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="918" mass="102850" checksum="633BA7EA6EB52FFC" modified="2018-12-05" version="1">ISVKKRNVKMPKSAKKPCTTQTSISRYFGGLSSSSRPQSGIKPNPAEHRLSRGKVRPGFCLSKITKSGGREGGKVAESPSDSSAPCRRSRSTYTPLEQQVLELKQQQKDVLLAVECGYKYRFFGEDAEIAAKELNITCHLDHNFMTCSIPTHRLFVHVRRLVSHGHKVGVVKQTETSALKASGTNRSALFSRQLSALYTKSTLVGEDVNPVCRVADVEEGGCADVVSDPPDSFLLCISENWDKVKKQLSVGVVVIRGSILTCGCCNKLRLLLLLFPSSAQTDDRIRVERRDSAEFEFTSAMNTVVKFYYSRSLSSVASFDSPVICCLGPLIQYLQEFNLEKVLRNESVRGSLLWVLDHTRTPFGRRLLRKWVSQPLTEPQCIAERQDAVQEMMESDSITLNSIRSLLSHLPDLERGICSIYHGKSSTQEFFLICSSLSRLGLELQALLPAVQSQIRSSLLRSLLLDTPDLLAPAHGFLKVLNEKAAKSGNKTELFSDLSGFPMLQERMSQIQSVLAEIQAHRKEVRLVLKAPALDYVTVSGQEFLIEVKNSLSSIVPPDWVKISSTKTVSRYHSPFLVERYKKLQQLREQLLLDCQREWTRFLDQFGEHYHTMKRAISHLATTDCLLSLAEVAKQGNYCRPKVCENNHQIMITDGRHPAIDLLMGEHSQYVPNHTVLQRDCKRTMIITGPNMGGKSSYIRQVALICVMAQIGSYVPACEACLGVLDGIYTRMGASDNIFKGRSTFMEELTEASEIISQATERSLVILDELGRGTSTHDGIAIAYATLEYFIKDVKSFTLFVTHYPPLCELEQVYPQHVSNFHMAFLLNEPDVTTDADEVQPEFITFLYQLTEGAAGRSYGLNVARLADIPDPILHTASRKARELELMVNARRKYQKLLSALWSICDKSSLEKWLQSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXA7</accession>
    <name>A0A3B5LXA7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sphingomyelin synthase-related protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Ceramide phosphoethanolamine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Sterile alpha motif domain-containing protein 8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Synthesizes sphingolipids through transfer of a phosphatidyl head group from a glycerophospholipid on to the primary hydroxyl of a ceramide in the lumen of the endoplasmic reticulum. Catalyzes the synthesis of ceramide phosphoethanolamines (CPEs) (such as N-acylsphing-4-enine 1-phosphoethanolamine) by transferring phosphoethanolamine head group, which is smaller and more hydrophilic than the phosphocholine (PC) headgroup transferred in the canonical sphingomyelin synthesis (SMS) reaction by SMS1 or SMS2, from a phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine, PE) to a ceramide (such as N-acylsphing-4-enine). The larger PC prevents an efficient fit in the enzyme's catalytic pocket, leading to little or no SMS activity. In vitro, in the absence of ceramide, it has PLC activity with preference for phosphatidylinositol and phosphatidic acid, but also hydrolyzes phosphatidylethanolamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N-hexadecanoyl-(4R)-hydroxysphinganine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine = N-hexadecanoyl-(4R)-hydroxysphinganine-1-phosphoethanolamine + a 1,2-diacyl-sn-glycerol</text>
            <dbReference type="Rhea" id="RHEA:42144"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:65107"/>
            <dbReference type="ChEBI" id="CHEBI:78656"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:42145"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N-hexadecanoylsphinganine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine = N-hexadecanoyl-sphinganine-1-phosphoethanolamine + a 1,2-diacyl-sn-glycerol</text>
            <dbReference type="Rhea" id="RHEA:42128"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:67042"/>
            <dbReference type="ChEBI" id="CHEBI:78654"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:42129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an N-acyl-(4R)-4-hydroxysphinganine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine = an N-acyl-(4R)-4-hydroxysphinganine-1-phosphoethanolamine + a 1,2-diacyl-sn-glycerol</text>
            <dbReference type="Rhea" id="RHEA:42148"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:31998"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:78657"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:42149"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>an N-acylsphing-4-enine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine = an N-acylsphing-4-enine 1-phosphoethanolamine + a 1,2-diacyl-sn-glycerol</text>
            <dbReference type="Rhea" id="RHEA:36079"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:73203"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:36080"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an N-acylsphinganine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine = an N-acylsphinganine-1-phosphoethanolamine + a 1,2-diacyl-sn-glycerol</text>
            <dbReference type="Rhea" id="RHEA:42136"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:31488"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:78655"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:42137"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingomyelin synthase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXA7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015787"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015982.1">
        <property type="protein sequence ID" value="ENSXCOP00000015787.1"/>
        <property type="gene ID" value="ENSXCOG00000011928.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155540"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047493">
        <property type="term" value="F:ceramide cholinephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033188">
        <property type="term" value="F:sphingomyelin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09515">
        <property type="entry name" value="SAM_SGMS1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000037">
        <property type="entry name" value="sphingomyelin synthase-related protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045221">
        <property type="entry name" value="Sphingomyelin_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025749">
        <property type="entry name" value="Sphingomyelin_synth-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290:SF25">
        <property type="entry name" value="SPHINGOMYELIN SYNTHASE-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21290">
        <property type="entry name" value="SPHINGOMYELIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14360">
        <property type="entry name" value="PAP2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="19">
        <location>
            <begin position="20"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00057029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00083488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="397" mass="45281" checksum="170A11C68DDA495E" modified="2018-12-05" version="1">MLCVSVCSRMSELNGRVSAWSSSQVAQWLQDEGFGQYVDLLCSQHRIDGVSLLALTEADLRDPPLSLTVLGHIKRLSVALRRLQRENKAELEELGLWPPDANSAGLSLALCQTHSNGRFRQPMVGRLDPEVWKTVISSVYVFLVCGLTSFVMVIVHERVPDMRTYPPLPDIFLDSVPRIPWAFVMAEACGLILCYMFLLILLLHKHRSILLRRLCSLMGTVFLLRCCTMFVTSLSVPGQHLKCASLSYGDTWGKIQRALAIWSGFGMTLTGVQTCGDYMFSGHTVVITMLNFFVTEYTPRNWNLIHTISWVLNLFGIFFILAAHEHYSIDVFIAFYITTRLFLYYHTLANTRAFQHSRRARIWFPMFSFFECNVNGPVPNQYHWPFSKPAFMKTLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXE0</accession>
    <name>A0A3B5LXE0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Exportin-T</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">Exportin(tRNA)</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 10">tRNA exportin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">tRNA nucleus export receptor which facilitates tRNA translocation across the nuclear pore complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <text evidence="10">Shuttles between the nucleus and the cytoplasm.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXE0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015817"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016012.1">
        <property type="protein sequence ID" value="ENSXCOP00000015817.1"/>
        <property type="gene ID" value="ENSXCOG00000011945.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007890"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071528">
        <property type="term" value="P:tRNA re-export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000105">
        <property type="entry name" value="Exportin for tRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045546">
        <property type="entry name" value="Exportin-T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040017">
        <property type="entry name" value="XPOT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15952:SF11">
        <property type="entry name" value="EXPORTIN-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15952">
        <property type="entry name" value="EXPORTIN-T/LOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19282">
        <property type="entry name" value="Exportin-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="5 10" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Exportin-1/Importin-beta-like" evidence="11">
        <location>
            <begin position="102"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Exportin-T C-terminal" evidence="12">
        <location>
            <begin position="328"/>
            <end position="938"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF19282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="939" mass="106715" checksum="FB879769ECC21442" modified="2018-12-05" version="1">MACQSVSAVMDEQALLGLNPNADAPYRQRLQALAYFEQLKESQGAWEVCADALAKGIYDVHVKFFCFQVLEHQIKFAQQQLIRETLMKWLQTQLMNAQPEKAFIRNKAAQVFALTFVMEYLTLWPKFFFDILNLVGLNPHGVDIYLRTLMAIDAEVVDRDIVHTSELQETRRNTLIKDSMREQCIPSLVESWFQILTAYQQTHPELTCQCLEVVGAYVSWIDLNLIANDRFVNLLLSQMSMEDLREEACDCLFEIVNKGMDPVDKTKLVESLCQVLQSAGFFNIEQEEDVDFLAKFSRLVNGMGQSLVLSWSKLVKSGNVKEATETLHAVEAKVPLLLQLLVHEDDDISTNMVGFCYDYLHVLKQQAVMLAVMKKLTYDDEYNFENEGEDEAMFVEYRKQLKMLLDRLAQVSPELLLEAVRRVFTSTMRRWQTAPFMEVEVAIRLLYMLGEALPASHGAHFSGDSAKTSALQDMMRTQLVSCGVSGYQHSSVSLEFFETVVRYDKFFIVEPQHIPNVLMAFLDQRGLRHSSPKVRSRVAYLFSRFIKTLKHMTAFIEDILTRIQDLLELAPPENGFLALLTSDDQLFMFEAAGVLIVSGESPVERKQVLMRSLLAPLTDAFRLLLTKLLQESVEEHQAALADCLSHAVGFARTSKAFSNKQTVKQCGCTEVYRDCLHSFLPALSCPVQRGALRSAVRSFLHRMIICLEEEVLPFIPAASEHMLKDCEAKDLQEFIPLISQITAKFKQIFMPLVLAIFEVLGRPAEENDQTAALEKQMLRRSYFAFIQTVAGSGMNEVLANQGAENIERVLFTIIQGAVEFPDPIAQKTCFIILTRLVELWGGKDGMVGFPDFTYKHIVPACFLAPLKPTFDLSDAQTVLTLSECALTLKMIHLKRGSEFIQFLQHEYLPSLQVTPEISQQELCQVLQQPDVKVLKNYMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXU5</accession>
    <name>A0A3B5LXU5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Bone morphogenetic protein 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXU5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015730"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015924.1">
        <property type="protein sequence ID" value="ENSXCOP00000015730.1"/>
        <property type="gene ID" value="ENSXCOG00000011884.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158644"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000003">
        <property type="entry name" value="Bone morphogenetic protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF139">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0891">Chondrogenesis</keyword>
    <keyword evidence="3" id="KW-0202">Cytokine</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 11" id="KW-0339">Growth factor</keyword>
    <keyword evidence="6" id="KW-0892">Osteogenesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="TGF-beta family profile" evidence="13">
        <location>
            <begin position="65"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="48"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="185" mass="20749" checksum="987BC06FF4A1AE28" modified="2018-12-05" version="1">MYYLFCKGRSINTKSAGIIGRNGPQSKQPFVVAFFKASGVLLRSVRAAGGKKKNHNRNKSTNQQESSRMPKPGDYNTSEQKQACKKHELYVSFRDLGWQDWIIAPEGYAAFYCDGECSFPLNAQMNATNHAIVQTLVHLMFPDNVPKPCCAPTKLNAISVLYFDDSSNVILKKYRNMVVRSCGCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYU1</accession>
    <name>A0A3B5LYU1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYU1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016110"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016313.1">
        <property type="protein sequence ID" value="ENSXCOP00000016110.1"/>
        <property type="gene ID" value="ENSXCOG00000012173.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157082"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050869">
        <property type="entry name" value="H3K4_H4K5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF193">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE SMYD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SET" evidence="3">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="230" mass="26661" checksum="53A3888F5F61F99D" modified="2018-12-05" version="1">MNHSCSPNVIVTYKGTVAEVRAVQEINPGEEIFDSYIDLLYPTEDRRERLLESYFFMCQCTECTTRSKDKAKTEVRKLSTPPEPKEIQSMVHYAKNVIEEFRRAKHYKNILELLEMCELSLEKMGAIFADTNVYMLHMMYQAMGVCLYMQDWDGAMSYGEKIVQPYSVHYPTYSLNVASMYLKLGRLYLGLEKKSQGVKALKKALAIMEVAHGKDHHYVAEVKREILDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZC0</accession>
    <name>A0A3B5LZC0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZC0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016719.1">
        <property type="protein sequence ID" value="ENSXCOP00000016512.1"/>
        <property type="gene ID" value="ENSXCOG00000012461.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01000000214661"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031696">
        <property type="entry name" value="DUF4719"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38505">
        <property type="entry name" value="HYPOTHETICAL PROTEIN LOC100362176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38505:SF1">
        <property type="entry name" value="RIKEN CDNA 1110032F04 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15843">
        <property type="entry name" value="DUF4719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017274859" evidence="3">
        <location>
            <begin position="27"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="213" mass="23174" checksum="107D0B7FBCC04843" modified="2018-12-05" version="1">MKPRLLGGGRTTRRLLSACLISACQALHSCGGVKCVDGHQCCLLPVPGNGSSGSAESCCKLPIHVFFDNVGWFTRKLSGILILLLLFAMGYFIQRIICPRPRHRNQNQEHAEEPSLFHGPTSASQDLLLEPYPECTLGDFASPNLPAYDEVKYLPTYEESMQEMHRDRDRSDDDLLSGAEPAATASGEQGRDVLGVSPGGEGGFAEIQTQVQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZK4</accession>
    <name>A0A3B5LZK4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Peroxisomal N(1)-acetyl-spermine/spermidine oxidase</fullName>
            <ecNumber evidence="16">1.5.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Polyamine oxidase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(1),N(12)-diacetylspermine + O2 + H2O = 3-acetamidopropanal + N(1)-acetylspermidine + H2O2</text>
            <dbReference type="Rhea" id="RHEA:25868"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:30322"/>
            <dbReference type="ChEBI" id="CHEBI:58324"/>
            <dbReference type="ChEBI" id="CHEBI:58550"/>
            <dbReference type="EC" id="1.5.3.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N(1)-acetylspermidine + O2 + H2O = 3-acetamidopropanal + putrescine + H2O2</text>
            <dbReference type="Rhea" id="RHEA:25812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:30322"/>
            <dbReference type="ChEBI" id="CHEBI:58324"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="1.5.3.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(1)-acetylspermine + O2 + H2O = 3-acetamidopropanal + spermidine + H2O2</text>
            <dbReference type="Rhea" id="RHEA:25800"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:30322"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:58101"/>
            <dbReference type="EC" id="1.5.3.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="15">Amine and polyamine metabolism; spermine metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.3.13" evidence="16"/>
    <dbReference type="RefSeq" id="XP_027873717.1">
        <property type="nucleotide sequence ID" value="XM_028017916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZK4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014786"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014972.1">
        <property type="protein sequence ID" value="ENSXCOP00000014786.1"/>
        <property type="gene ID" value="ENSXCOG00000011211.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114144763"/>
    <dbReference type="GeneTree" id="ENSGT00940000157511"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052903">
        <property type="term" value="F:N(1)-acetylpolyamine oxidase (3-acetamidopropanal-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046208">
        <property type="term" value="P:spermine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.660.10:FF:000008">
        <property type="entry name" value="Spermine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.660.10:FF:000003">
        <property type="entry name" value="spermine oxidase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF393">
        <property type="entry name" value="SPERMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="20">
        <location>
            <begin position="34"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Amine oxidase" evidence="20">
        <location>
            <begin position="308"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="547" mass="61651" checksum="99B90E979855527F" modified="2018-12-05" version="1">MQSCEISSDSTDDPLSSGFRPHRQPRIVVIGAGLAGLAATRVLLKNGFTDVTVLEASDHIGGRVQSVKNGEATLELGATWIHGANGNPVYHLAEENGLLEHTTDGERSVGRISLYTKNGVAHYQTNVGKRIPKDLVEEFSDLYNEVYELTQEFFQNGKPVCAESQNSVGIFTRDLVRKRIMVDPDDSESTKKLKLSMLQQYLKVESCESSSPSMDEVSLSEFGEWTEIPGAHYVIPEGFVKIVDLLAQDIPSHTICLCKPVRCIHWNYSAQHREVIGKGSHVKMEDNHNDNNHSHQRNEEPLILGQPIWVECEDEEWIPADHVIVTTSLGVLKQNHEAMFSPSLPEDKVLAIEKLGISTTDKIFLEFEEPFWSPECNSIQFVWEDEAQLEQLAYPEDLWYKKICSFDVLYPPERYGYMLSGWICGQEALHMERCDDETVAETCTKLLRRFTGNPDIPKPKRILRSSWGSNPYIRGSYSFTRVGSSGQDCERLAMPLPYANSTKAPLLQVLFAGEATHRKYYSTTHGALLSGQREATRLMEVYHDHKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZL3</accession>
    <name>A0A3B5LZL3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZL3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017529.1">
        <property type="protein sequence ID" value="ENSXCOP00000017308.1"/>
        <property type="gene ID" value="ENSXCOG00000013047.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000235333"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="105" mass="12017" checksum="FBD88401037B9420" modified="2018-12-05" version="1">MPHFTVVPVKDQAQSSYDSLEGINWVDYRDTGQDCPDHQDGADGKSHNFYSLFLTEMESQLEDLSLNGYPFTLLYFTYKSQHKQVNSCLGSFVNIWLNGYHGNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M048</accession>
    <name>A0A3B5M048_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Hypoxanthine phosphoribosyltransferase</fullName>
            <ecNumber evidence="16">2.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:25424"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:25426"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17973"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:17975"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.8" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M048"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016845.1">
        <property type="protein sequence ID" value="ENSXCOP00000016630.1"/>
        <property type="gene ID" value="ENSXCOG00000011799.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155028"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00648"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052657">
        <property type="term" value="F:guanine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032263">
        <property type="term" value="P:GMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006178">
        <property type="term" value="P:guanine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046100">
        <property type="term" value="P:hypoxanthine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000019">
        <property type="entry name" value="Hypoxanthine phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050408">
        <property type="entry name" value="HGPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005904">
        <property type="entry name" value="Hxn_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01203">
        <property type="entry name" value="HGPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43340">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43340:SF6">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 16" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 16" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="17">
        <location>
            <begin position="48"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU364099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="225" mass="25453" checksum="70D3FD4580AFDFB0" modified="2018-12-05" version="1">MNDCPSAPSLASFLGEILDEEQGYDLDLFCIPKHYAADLEKVYIPHGLILDRTERLAREIMKEMGGHHIVALCVLKGGYKFFADLLDYIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGEIKVIGGDDLSTLTGKNVLIVEDIIDTGKTMKTLLQLLKQYNPKMVKVASLLVKRTPRSVGYRPDFVGFEVPDKFVVGYALDYNEYFRDLNHICVISETGKEKYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M253</accession>
    <name>A0A3B5M253_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M253"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017591.1">
        <property type="protein sequence ID" value="ENSXCOP00000017370.1"/>
        <property type="gene ID" value="ENSXCOG00000013093.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00400000024060"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19446">
        <property type="entry name" value="REVERSE TRANSCRIPTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="154"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Reverse transcriptase" evidence="4">
        <location>
            <begin position="444"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1140" mass="127877" checksum="2BF8E2F73B7F6E0F" modified="2018-12-05" version="1">MASRSALKMTSIPSMTFHNSSLEKEEEMNYDFYEQIKSLVESDDSSDDFTEDDEDVEESFLDISAEEPVLGKFPIDTKGTITVVLPSLEYICVICKQHMGKASELVAHFNIKHRDIPLVFKCAKCDKTNSNHRSIACHAPKCGGIKLTEESLPMVCECCQARFATLSGLSQHKRHAHPVTRNEERIKDGIKGTSQRGVHRSCWSLKEVEHKKTGAPMSDSLHFSSRPLETLSPPPNVTTGTSSILAQAAERLTNENSGTLEKPAMEAIKAWLNGEGQHDALVETATALMLCPMRLVKNKGKRSKPENDIIKPRILPTRSWMKKRAEKRGSFMKHQKLFFKNRSLLASLVLDGTERHECRIPNADVYRFYCEKWEKVLPFNGLGQFKSSGVANNEYFEPLISVEEVQTAIRAIKPTSAAGPDGLTRAAICAADPEGRTLTALFNAWMITGIIPKELKKNRTILIPKVMDDEKLKELGNWRPITIGSMILRLFSRIMTARLARACPLNPRQRGFIAASGCSENLKVLQDLMRHAKKLHRPLAVMFIDIAKAFDSVSHAHILWVLRHKKVDEHVVGIIQNAYDRCTTSFKSNGESTREISIRVGVKQGDPMSPLLFNLAMDPLICTLESHGVGYSIDTDHVTALAFADDLVLVSESWVGMAANLAILESFCGLSGLEVQARKCQGFMISPTKDSYTVNNCDPWTIKNKDVHMIQPDESTKYLGLKIGPWTGTIQPDLHCQLKTWISKIDEAPLKPTQKVELLNAYALPRLLYPADHSDCKQSTLRGLDQEIRKAVKGWLHLPASTCDGLLYARARDGGLAILKLEKTIPSVQVRRLQRIANSSDAITRNIVSSQGMEEEYRNLWVRAGGDSDAIPTFVLRDSVSKEPVYPRPCDWREHEFQRWCEKPVQGRGIVNFAHDRISNAWLRPWCGFKQCFFIAALQLRANIYPTRESLNRGRDGALRSCRKCSARLESLSHILGQCPAVQKARIARHNKISNILADEAARLGWSVYKEPRFISETGEMRKPDLVFAKDEEAVVIDVTVRFELSKNTLSDAASHKVSYYTPLGDQVKVLTKASKVTFFGFPVGARGKWPLENNEVLTSLGLTKPRTTSLAKMISRRTLLFSLDILRMFCGNYAVYEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2N1</accession>
    <name>A0A3B5M2N1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M2N1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014269"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014446.1">
        <property type="protein sequence ID" value="ENSXCOP00000014269.1"/>
        <property type="gene ID" value="ENSXCOG00000010811.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155062"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000551">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000686">
        <property type="entry name" value="Ribosomal protein S6 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="50"/>
            <end position="343"/>
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        <location>
            <begin position="249"/>
            <end position="319"/>
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            <end position="401"/>
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            <begin position="277"/>
            <end position="288"/>
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        <location>
            <begin position="367"/>
            <end position="377"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="391"/>
            <end position="401"/>
        </location>
    </feature>
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        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00009804"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
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            <dbReference type="ARBA" id="ARBA00022527"/>
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    <evidence type="ECO:0000256" key="13">
        <source>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014269.1"/>
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    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="401" mass="44980" checksum="EB5C8A1CB3974BBF" modified="2018-12-05" version="1">MAGVFDIDLETEDGSDTEVSNDWITGQTEEVELTSENVNRDSERVGPDCFELLTVLGKGAYGKVFQVRKVQGPQTGKIFAMKVLKKAKIVRNAKDTAHTRAEREILEKVRHPFIVDLLYAFQTGGKLYLILECLSGGELFMQLEKEGIFMEDTACFYLGEITMALGHLHSNGIIYRDLKPENIMLCHQGHIKLTDFGLSKESIHDGTITHTFCGTIEYMWVTLLKKNPAQRLGSGKDDAADIQKHPFFRHISWEELLNKRVDPPYKPQLHSEEDVSQFDTRFTRQTPVDSPDDTRLSHSAELAFAGFSFVAPSVLESLKEGFSFEPRSRHVRRHNSSPRTPISPLKFSPAGPFKSSMDGEADLFSPTSPPAAPPPGPLENGAASRPIKTPSRNKKKKEHRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4G7</accession>
    <name>A0A3B5M4G7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Usher syndrome 2A (autosomal recessive, mild)</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000016316"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016522.1">
        <property type="protein sequence ID" value="ENSXCOP00000016316.1"/>
        <property type="gene ID" value="ENSXCOG00000012306.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158456"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="8"/>
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        <property type="match status" value="24"/>
    </dbReference>
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        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="laminin subunit alpha"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="usherin"/>
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        <property type="entry name" value="Galactose-binding domain-like"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="25"/>
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        <property type="entry name" value="ConA-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR003961">
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        <property type="entry name" value="Laminin_N"/>
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        <property type="entry name" value="LE_dom"/>
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    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
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    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF7">
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    </dbReference>
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        <property type="match status" value="15"/>
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    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
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        <property type="match status" value="5"/>
    </dbReference>
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        <property type="match status" value="25"/>
    </dbReference>
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        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 10" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="4613"/>
            <end position="4635"/>
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    </feature>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6K5</accession>
    <name>A0A3B5M6K5_9TELE</name>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
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            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="231" mass="25175" checksum="A832E9C059CEACDC" modified="2018-12-05" version="1">MVGLRNAGVLGASAVLAGGVAYLIWSYGSSRKEKKLETRPGEDGRNGREEEDEVEARPVESEGTQVLVLGLDGAGKTSLLHCFSSGSLEQDVEPTQGLNAISISKDDMDIEFLEIGGGENLRPYWQKYTPKALLLVFVVDSANPELFPVTKKHLHELLVLHPHLPLMVLANKQDLPTSCNITDLHDALSLSEAGDRRLFLIGTHVRKGDSELCSGVQDARDLIFQMVSGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M990</accession>
    <name>A0A3B5M990_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CRIB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CDC42SE/SPEC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M990"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016329"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016535.1">
        <property type="protein sequence ID" value="ENSXCOP00000016329.1"/>
        <property type="gene ID" value="ENSXCOG00000012337.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158245"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00132">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039056">
        <property type="entry name" value="SPEC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13502">
        <property type="entry name" value="CDC42 SMALL EFFECTOR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13502:SF7">
        <property type="entry name" value="CRIB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIB" evidence="11">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="91" mass="10309" checksum="271B6358F15DCB13" modified="2018-12-05" version="1">MTEFWVCFSCCIAEQPQPKRRRRIDRSMIGEPTNFVHTTHVGSGDMGHGLASVSFIHCSRFNRLNSPHCDHWLLNISNLFVGRSCSGSDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9G8</accession>
    <name>A0A3B5M9G8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9G8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020918"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021175.1">
        <property type="protein sequence ID" value="ENSXCOP00000020918.1"/>
        <property type="gene ID" value="ENSXCOG00000015660.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047764"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03157">
        <property type="entry name" value="TM4SF12_like_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF462">
        <property type="entry name" value="TETRASPANIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017288377" description="Tetraspanin" evidence="13">
        <location>
            <begin position="19"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="92"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="224"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="256"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="271"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="158"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="285" mass="31732" checksum="B3C08E86982BA16D" modified="2018-12-05" version="1">VCLSLSVCLSMCVSLSLSFSVCEQLMSVSVLGVAAWIRDTLNSVLTLTAHTRLEEAAVRSYSAAVHPVFISVCCLLLILSMLGYCGALRCRLLLLCWYFCSLLVIFCVELASAVWTYDEPSVQRADMISLKSRMPDYSLQRYQWLTHTWDSFQTQFQCCGVIYFTDWLEMTEMEFPPDSCCTNQFPGCAGRAHQHDLSDLHQEGCGLKVYRFIRGTKPLQVLRFLGVSIGVAQILAMALTLTLLWALYYGHHHQQPDSYGTPRPPGESGSVVGTNGTSVNDRVGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9Y3</accession>
    <name>A0A3B5M9Y3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="4">IMP dehydrogenase</shortName>
            <shortName evidence="4">IMPD</shortName>
            <shortName evidence="4">IMPDH</shortName>
            <ecNumber evidence="4 10">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">IMPDH</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 10">
            <text>IMP + NAD(+) + H2O = XMP + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="4">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 10">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="reaction ID" value="UER00295"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="F:IMP dehydrogenase activity"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="entry name" value="Inosine-5'-monophosphate dehydrogenase 2"/>
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        <property type="entry name" value="Aldolase class I"/>
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        <property type="entry name" value="Aldolase_TIM"/>
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        <property type="entry name" value="INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE 1B"/>
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        <property type="entry name" value="INOSINE-5-MONOPHOSPHATE DEHYDROGENASE RELATED"/>
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        <location>
            <begin position="114"/>
            <end position="175"/>
        </location>
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        <location>
            <begin position="179"/>
            <end position="237"/>
        </location>
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        <location>
            <position position="331"/>
        </location>
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        <location>
            <position position="429"/>
        </location>
    </feature>
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        <location>
            <begin position="274"/>
            <end position="276"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
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        <location>
            <begin position="324"/>
            <end position="326"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:57540"/>
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        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
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        </location>
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            <name>IMP</name>
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        <source>
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    </evidence>
    <sequence length="481" mass="52307" checksum="114FF56935CE8823" modified="2018-12-05" version="1">MADYLISGGTGYIPDDGLSAQQLFSVGDGLTYNDFLILPGFIDFTSDEVDLTSALTKKITLKTPLISSPMDTVSESAMAIAMALMGGMGFIHHNCTPEFQANEVRKVKRFEQGFITDPVVMSLRHTIGDVFEAKVRHGFSGIPITETGKMGSKLVGIVTSRDIDFLSEKDHGKPLEEAMTKREDLVVAPAGVTLKEANDILQRSKKGKLPIVNDNDELVAIIARTDLKKNRDYPLASKDSRKQLLCGAAIGTREDDKYRLDLLVQAGVDVVVLDSSQGNSVYQINMISYIKQKHPELQVVGGNVVTAAQAKNLIDAGVDALRVGMGCGSICITQEVMACGRPQGTSVYKVAEYARRFGVPVIADGGIQTVGHVVKALSLGASTVMMGSLLAATTEAPGEYFFSDGVRLKKYRGMGSLDAMEKSTSSQKRYFSLKRVMSSLQFIFFPSSMMYSGELKFEKRTMSAQVEGGVHGLHSYEKRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MB02</accession>
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        <submittedName>
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        <name type="scientific">Xiphophorus couchianus</name>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="137" mass="15668" checksum="1C69858EB7A6407D" modified="2018-12-05" version="1">MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDIFYDLVRQINRKTPVEKKKAKKKSNCVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MD99</accession>
    <name>A0A3B5MD99_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">COMM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
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        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MD99"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017679"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017903.1">
        <property type="protein sequence ID" value="ENSXCOP00000017679.1"/>
        <property type="gene ID" value="ENSXCOG00000013329.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037357">
        <property type="entry name" value="COMMD5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15666">
        <property type="entry name" value="COMM DOMAIN CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15666:SF1">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="14692" checksum="1740412426B19E4E" modified="2018-12-05" version="1">MMQDFLGGRIPPEIESMSKNLKDVDQELFRKLLKVVVSALEGKDCREVMKSIAESSVIPQERLSHIIAGMHRVLSEAIRIPASLLKQEANGYKFIYDMIKDSVVILDVKLLVKLSRFQQVFTNCWWLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MG24</accession>
    <name>A0A3B5MG24_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">ABC-type oligopeptide transporter ABCB9</fullName>
            <ecNumber evidence="16">7.4.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">ATP-binding cassette sub-family B member 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">ATP-binding cassette transporter 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">TAP-like protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">ATP-dependent low-affinity peptide transporter which translocates a broad spectrum of peptides from the cytosol to the lysosomal lumen for degradation. Displays a broad peptide length specificity from 6-mer up to at least 59-mer peptides with an optimum of 23-mers. Binds and transports smaller and larger peptides with the same affinity. Favors positively charged, aromatic or hydrophobic residues in the N- and C-terminal positions whereas negatively charged residues as well as asparagine and methionine are not favored.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a [oligopeptide](in) + ATP + H2O = a [oligopeptide](out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14429"/>
            <dbReference type="Rhea" id="RHEA-COMP:10531"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.4.2.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:14430"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer. Interacts (via TMD0 region) with LAMP1; this interaction strongly stabilizes ABCB9 and protects ABCB9 against lysosomal degradation. Interacts (via TMD0 region) with LAMP2 (isoform LAMP-2B). Interacts (via TMD0) with YIF1B; this interaction allows (but is not essential) the ER-to-Golgi trafficking and strongly depends on a salt bridge within TMD0.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.4.2.6" evidence="16"/>
    <dbReference type="RefSeq" id="XP_027889092.1">
        <property type="nucleotide sequence ID" value="XM_028033291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MG24"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020066"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020313.1">
        <property type="protein sequence ID" value="ENSXCOP00000020066.1"/>
        <property type="gene ID" value="ENSXCOG00000015051.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114154309"/>
    <dbReference type="KEGG" id="xco:114154309"/>
    <dbReference type="CTD" id="23457"/>
    <dbReference type="GeneTree" id="ENSGT00940000155431"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03249">
        <property type="entry name" value="ABC_MTABC3_MDL1_MDL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000031">
        <property type="entry name" value="ATP-binding cassette sub-family B member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000140">
        <property type="entry name" value="Lipid A export ATP-binding/permease protein MsbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF19">
        <property type="entry name" value="ABC TRANSPORTER B FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002773">
        <property type="entry name" value="ABC_prm/ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="11 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="202"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="246"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="345"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="24">
        <location>
            <begin position="207"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="23">
        <location>
            <begin position="521"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="144"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="774"/>
            <end position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00068474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00079330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00083142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00084061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="801" mass="88425" checksum="C4DF28946133B6DF" modified="2018-12-05" version="1">MGIHAPVICTLLYVVLDSVITTVLYIKGTELSLFIRDVLDFNILSSVLDLWGIVLLRASLLLGASIGVLWNREDGPPRVARVTTIILLFCMVVITYTLVKLLMLTEVEPLSRQPWILSLMVWTCASSLGVLLLWRRLGRVSSSESGLNVRNSRGGGGRVSEDTEKLVDTAGEEEEAGGRKEKEKVSSRATLGRLLSYCRKDAGLLSLAVLFLFVSAVCEAFIPLYYGKAIDSIVVHQSMEYFAKPVATLASLALVSSMAIGVRGGVFTLTMARLNLRLRSRLFRTLMTQEIAFFDENHTGDILSRLSADTTQVSDLISQNVNVFLRSVIKGIGFIIFMFRMSWKLTLVTMMGFPFIALVSKFYGEYYKKLTKEVQTVLAEANKVAEETISAMRTVRSFANESGEADSYYAKLLVMFQLNKKQALAYACYMWSSCISELALEVAVLYYGGHLVLTDQLSSGGLISFFIYMLELGECFESIASVYTGLMQGVGAAEKVFEYLDRKPKHPAEGTEAPNSCTGLVEFRDITFAYPTRPEVDVLKGVSFTLRPGEVTALVGPSGSGKSSCVSLLENFYLPQRGQVLLDGNPVNEFQHDYLHSKIALVGQEPVLFARTIKENISYGLSDVSMEMVVQAATKANAHDFITSLPKGYDTSVGEKGLQLSGGQKQRVAIARALIRQPRVLILDEATSALDAESEHNVQQALNRIMQQHTVLVIAHRLSTVEKADNIIVIDRGRVAERGNHSQLMATGGLYCKLVQRQVLGIETGAEDLNPSETVRWKSDRGRRRQSTGSSGSESEHNMRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MH26</accession>
    <name>A0A3B5MH26_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Laminin G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MH26"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023057"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023334.1">
        <property type="protein sequence ID" value="ENSXCOP00000023057.1"/>
        <property type="gene ID" value="ENSXCOG00000017232.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155978"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
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        <property type="entry name" value="NEUREXIN 2A ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Laminin G" evidence="2">
        <location>
            <begin position="17"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="170" mass="18546" checksum="6741ED84EFE2DF12" modified="2018-12-05" version="1">NSDKHVSFPLSGQQESVATFKGNEFFSYNLSTTPIQSSLDEITLSFRTLQRNGLLLHTGKSADYVNLSLRNGAVWLVINLGSGAFEALVEPASGKFNDNVWHDVRVTRNLRQVTILVDGILTTTGYTQENYTMLGSDDLFYIGGSLNTADLPGSPVSNNFMGCLKDVSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHB8</accession>
    <name>A0A3B5MHB8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHB8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018969"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019206.1">
        <property type="protein sequence ID" value="ENSXCOP00000018969.1"/>
        <property type="gene ID" value="ENSXCOG00000014264.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="F-box only protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000002">
        <property type="entry name" value="F-box only protein 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007397">
        <property type="entry name" value="F-box-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039752">
        <property type="entry name" value="F-box_only"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12125:SF12">
        <property type="entry name" value="F-BOX ONLY PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12125">
        <property type="entry name" value="F-BOX ONLY PROTEIN 6-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04300">
        <property type="entry name" value="FBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01198">
        <property type="entry name" value="FBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51114">
        <property type="entry name" value="FBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="4"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="FBA" evidence="3">
        <location>
            <begin position="72"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="253" mass="29978" checksum="DEA7026D34C082E2" modified="2018-12-05" version="1">MLLSHQAIPLPLDIMEEIFLNLPPQHVVCVCRLVCRQWKDVADSESFWRERCRREGYQLRDPSKVPSNWRLFYFLCKKRRNLIKNPRAEDEFRGWRILRNGGHKWKIEGTFGQHSNPAVQKNYVTSFDWCTKEQVINLKDEGYNPSFMDEFQPHIRISDWYGARSDCGSIYIISVQLLDHKKNVLQNFSPEPVNIPQWNDQQWHQMVHVFKDYGPGVRYVRFTHGGKDTQFWAGWYGIRVTESCVEVCPALDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJ64</accession>
    <name>A0A3B5MJ64_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Somatotropin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Growth hormone</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the somatotropin/prolactin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJ64"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024373"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024666.1">
        <property type="protein sequence ID" value="ENSXCOP00000024373.1"/>
        <property type="gene ID" value="ENSXCOG00000018198.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169702"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070186">
        <property type="term" value="F:growth hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005131">
        <property type="term" value="F:growth hormone receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060396">
        <property type="term" value="P:growth hormone receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045927">
        <property type="term" value="P:positive regulation of growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046427">
        <property type="term" value="P:positive regulation of receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031667">
        <property type="term" value="P:response to nutrient levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001400">
        <property type="entry name" value="Somatotropin/Prolactin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018116">
        <property type="entry name" value="Somatotropin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11417:SF2">
        <property type="entry name" value="SOMATOTROPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11417">
        <property type="entry name" value="SOMATOTROPIN,PROLACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00103">
        <property type="entry name" value="Hormone_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00836">
        <property type="entry name" value="SOMATOTROPIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00338">
        <property type="entry name" value="SOMATOTROPIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 8" id="KW-0372">Hormone</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="175" mass="19992" checksum="0AB7C7F0444ADD40" modified="2018-12-05" version="1">MANLYTIQQSIILYYNNNNKNRILHSITNYGWLQHSTSKTLVLASESFVDPLCIDWLQVLKLLSISYRLVESWEFPSRSLSGGSGPRAQISPKLTELKMGIMLLIKANQDVPEIFTDTSTLQLAPYGNYYQSLEADESLRRTYELLACFKKDMHKVETYLTVAKCRLSPEANCTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJU5</accession>
    <name>A0A3B5MJU5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the immunoglobulin superfamily. Turtle family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSXCOT00000024343.1">
        <property type="protein sequence ID" value="ENSXCOP00000024057.1"/>
        <property type="gene ID" value="ENSXCOG00000017964.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155900"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000272">
        <property type="entry name" value="Immunoglobulin superfamily member 9B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin superfamily member 9B"/>
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        <property type="entry name" value="protein turtle homolog B"/>
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        <property type="entry name" value="protein turtle homolog B isoform X2"/>
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        <property type="entry name" value="protein turtle homolog B isoform X2"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
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        <property type="entry name" value="FN3_dom"/>
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        <property type="entry name" value="FN3_sf"/>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Neural/epithelial_adhesion"/>
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        <property type="entry name" value="CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN TURTLE HOMOLOG B"/>
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        <property type="entry name" value="IGc2"/>
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        <property type="entry name" value="Fibronectin type III"/>
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        <property type="entry name" value="IG_LIKE"/>
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    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="691"/>
            <end position="716"/>
        </location>
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            <begin position="1"/>
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            <end position="571"/>
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            <begin position="1160"/>
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        <location>
            <begin position="761"/>
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            <begin position="1067"/>
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            <begin position="1246"/>
            <end position="1259"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00061549"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
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            <dbReference type="PROSITE" id="PS50853"/>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024057.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1288" mass="142991" checksum="FAE5ABD75443713B" modified="2018-12-05" version="1">FVTARARESVILGCDVPHPLNGKPYVVEWFKYGMPIPFFINFRFYPPHVDPEYAGRARLHGKSSLQIENLRSDDQGWYECKVLMLEQQYDTFHNGSWVHLTVNAPPTFTDTPPQYVEAKERRSITLSCTAFGNPKPSVSWLREGRLLISSAKYKVSDGNLTVLSITREDRGAYTCRAFSSQGEAIHTTRLLVQGPPFIISPPENITVNISQDAFFTCQAEAYPRNLTYTWFWEEDNVFFKNDLKRRVSILIDGSLIIVQVKPEDTGKYTCSPSNSLGTPPSASAYLAVQYPARVVNMPSVIYVAIGLPGFIRCPVDANPPLTLVKWKKDGLPLRIDKYPGWSQIDDGSIRVAEVTEDSLGTYTCMPYNALGSMGWSPPAPLVLKDPPKFSVVPGGEYRQEAGRELVIPCEAEGDPFPNITWRKVGKPSKSKHNVLPRGSLQLKSLTKDDHGEWECVATNVATSITASTHVQVIGTSPHAPTKVRVATSSTWANMSWEASYDGGFQQTYSVWLKRERLGPHDWVSIPVPGGQDWMVVPGLEPETTYQFSVLAQNKLGTGPFSEVVTVNTLVFPTSTPEPLVLLTPPRCLTANRTQQGVLLTWIPPANHTAPIQHYVMEFRLGERWEILDDSIPAGELELLARDLIQEAWYEFRVMAVMDDMVSEPSNVVGVSSTDFFPPPEMVEERGLTRPVVAGIVATICFLAAAVLFSTMAACFVNKQRRKKLKKKKDPPLSITHLRKSMSSSGKVSPESIRSKASASESSKESHDQRGRKPPPSPGRKEELSLYKKTKRAITSKRYMSKHEAEPTTPIELISRGPDGRFVIDPTDAVSVKPRRIEGFPFVEESDLYPEFRQSDEENDDPRPLPPVMAPLRPHQISPISSQESYLQPPAYSPRFHRPFEGMTIMESSQLHATGQIRGSLSHRAFYGYLGPDHDPPPPFYMPDTSPLRSVMSSPPYFAEGPFAHHMIPEEIGESEFPQYAVSGFPLPLTLTSSSHSPEIWQGLDFTFSSLERPQFIFPPHHLLHTRHDPPYASPPHVLPLSAFQHSSLPMPTYPTVLPLEAPSGRMSKSHKSKSPSKARPHGSPARHLAMQEGHLGQLRPSGHSMSVPVLSYTDPMAHIVPSKFSNLDTRWFETTPGFSPRQTRRMDSGLHQVVLQPSRLSPLTQSPLSSQEGSPEIVVRPRPRPSIVQPPIPPGMSEITLLPPSTASFSRRSSPSSSPAQGQGSRRTSPSYRSHMAFATAATSYPASQSPSPPMESSSIFGQMPAHRRTEEEILPSEPSPPQLSASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MM01</accession>
    <name>A0A3B5MM01_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Probable proton-coupled zinc antiporter SLC30A4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Solute carrier family 30 member 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Zinc transporter 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Probable proton-coupled zinc ion antiporter mediating zinc import from cytoplasm potentially into the endocytic compartment. Controls zinc deposition in milk.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Zn(2+)(in) + 2 H(+)(out) = Zn(2+)(out) + 2 H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72627"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MM01"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024591"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024888.1">
        <property type="protein sequence ID" value="ENSXCOP00000024591.1"/>
        <property type="gene ID" value="ENSXCOG00000018370.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157545"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010043">
        <property type="term" value="P:response to zinc ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000017">
        <property type="entry name" value="zinc transporter 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050681">
        <property type="entry name" value="CDF/SLC30A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562">
        <property type="entry name" value="CATION EFFLUX PROTEIN/ ZINC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562:SF27">
        <property type="entry name" value="PROTON-COUPLED ZINC ANTIPORTER SLC30A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-0458">Lysosome</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0864">Zinc transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="272"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="22">
        <location>
            <begin position="125"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="23">
        <location>
            <begin position="339"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00077288"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="Pfam" id="PF16916"/>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="432" mass="47222" checksum="D6841E269CEFE691" modified="2018-12-05" version="1">MSGGKLFGKVRSAFRRDRSDWSLSDSAPFDFSDELVEDDEAPKFNKLKVVVSGEMSDYSAGAPSNGVAVSTVVVPPGGGGDEEEDDDSLLDSSSTLGSPGLNMDPCDNCNKKRERIRHRRVMKRLIVAAVLYLLFMTGEIIGGYVSNSLAIMTDAVHLLADVIGILFSLLALWLSTKPPTKRFTFGLHRLEVVSAVLSVLLIYILTGFLLYEAVLRTVHQDFDIDGDVMLITAAVGVAVNLIMGFLLNQGGHLHSHGSHSAGSGQQRPSGSLAVRAAFIHALGDLLQSVGVLIAAYVVRFKPELKLADPICTYIFSVLVLFTTVRIIRDTIVIVLEGVPRHLDTQRIKEDLLKLEDVQSVDELNVWALTADKTAALVHLQLTPSSANNWEDVQAKARHLLLHTYGLTRCTVQVQSHRQRTVRNCAQCQQPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MM31</accession>
    <name>A0A3B5MM31_9TELE</name>
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        <submittedName>
            <fullName evidence="6">Innate immunity activator b</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Neoteleostei</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MM31"/>
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        <property type="protein sequence ID" value="ENSXCOP00000024843.1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="CCDC120/INAVA"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="INNATE IMMUNITY ACTIVATOR B"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytohesin Ubiquitin Protein Inducing" evidence="5">
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            <begin position="2"/>
            <end position="136"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00004496"/>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MMY7</accession>
    <name>A0A3B5MMY7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Atherinomorphae</taxon>
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    </dbReference>
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            <dbReference type="Proteomes" id="UP000261380"/>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="58" mass="6309" checksum="3072CA6F39824A59" modified="2018-12-05" version="1">MDNIISPDIDECSAPEPEDGSGPLCSQICLNTLGSYLCACHHGYELRSDERTCICKFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPS2</accession>
    <name>A0A3B5MPS2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">AXH domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <lineage>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATXN1 family.</text>
    </comment>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020997">
        <property type="entry name" value="Ataxin-1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043404">
        <property type="entry name" value="ATAXIN1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003652">
        <property type="entry name" value="Ataxin_AXH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036096">
        <property type="entry name" value="Ataxin_AXH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13392">
        <property type="entry name" value="ATAXIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13392:SF5">
        <property type="entry name" value="ATAXIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12547">
        <property type="entry name" value="ATXN-1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08517">
        <property type="entry name" value="AXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00536">
        <property type="entry name" value="AXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102031">
        <property type="entry name" value="AXH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51148">
        <property type="entry name" value="AXH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AXH" evidence="10">
        <location>
            <begin position="497"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="672"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="675"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="725" mass="76734" checksum="DF874F9341486C6C" modified="2018-12-05" version="1">MKANQERSNGCLPPKKREILALEQRPVVVAATTPPVAVPTDSPHTENLAWLASVASERCKSRETESPRCPIPSTSSSSPTTSIPSPATTLSAVPLASLPAVYPAALPQQAGTIQFTQLGPNVQFISSGPYAGYISSHIIPTNATSAPNSSALVGQRTQIDGYTTALISPSTKGEQQFQIGLSPSELAPVTLPSSPQVTNQYIHLDNRTPLTVNGNAVASPAAHLHLHRHAAVLPQTLTLAPSQLVVQYADGAAGKKPEGHAKSVLNGEVEVVKQAKVSGANHQPVQSYEARHFLLPADYGQNPAGLQTSLVLVAQPNHGVEHDAGSNKISLVQTEKGSICLGKPISRASSFSSLSSSEVVKSVPHTVIQTSLPPEELPVSLYSSTQPPIIGYITSANQHTVSYHAALPQHLVIPSGQSLLIPVSSANNGTEIEVSRTVSAITATTPQISTAMPHAYLATALSKCEALVPAVVAAPSQAPTPVPIPSPSSVQASPSVSSPSSPVALPPFFMRGSIIQLADGELKRVEDLKTEDFIQSAEISSELKIDSSTVERIDCGQSPNAVLIQFSVGELKAQVCVEVLVEYPFFVFGQGWSSCCPDRTTQLFELSCAKLCVGDVCLKMGSLSDLSHCSERMFRGGPMLAGSGGAEVRQEPLKTDISALCSDSERPFVRKRRWSAPERDQTEKAEEEPPVTLPKPSFLPQEVKISIEGHSSAGGERFLKKRVDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRM5</accession>
    <name>A0A3B5MRM5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein S100</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">S100 calcium-binding protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the S-100 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027868096.1">
        <property type="nucleotide sequence ID" value="XM_028012295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRM5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026156"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026472.1">
        <property type="protein sequence ID" value="ENSXCOP00000026156.1"/>
        <property type="gene ID" value="ENSXCOG00000019540.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114141636"/>
    <dbReference type="KEGG" id="xco:114141636"/>
    <dbReference type="CTD" id="406276"/>
    <dbReference type="GeneTree" id="ENSGT00940000162189"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000044">
        <property type="entry name" value="Protein S100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001751">
        <property type="entry name" value="S100/CaBP7/8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013787">
        <property type="entry name" value="S100_Ca-bd_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639:SF134">
        <property type="entry name" value="PROTEIN S100-A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639">
        <property type="entry name" value="S100 CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01023">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01394">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00303">
        <property type="entry name" value="S100_CABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="49"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="99" mass="11395" checksum="2E724DC070C49D14" modified="2018-12-05" version="1">MTELEKSMESLITVFHRYAKEGGNKTTLSKKELKKLIENELPNFLTTQKNPDTVSCIIKDLDQNKDDELDFEEFVPFVAGLTIACEKHFALHHEKKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRR5</accession>
    <name>A0A3B5MRR5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CUB domain containing protein 1a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRR5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026514.1">
        <property type="protein sequence ID" value="ENSXCOP00000026196.1"/>
        <property type="gene ID" value="ENSXCOG00000019569.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010209"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038811">
        <property type="entry name" value="CDCP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056266">
        <property type="entry name" value="CDCP1_CUB_3rd_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056269">
        <property type="entry name" value="CUB_CDCP1_2nd_5th"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14477">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14477:SF1">
        <property type="entry name" value="CUB DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23665">
        <property type="entry name" value="CDCP1_CUB_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23668">
        <property type="entry name" value="CUB_CDCP1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="588"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="CDCP1 second and fifth CUB" evidence="4">
        <location>
            <begin position="66"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="CDCP1 third and sixth CUB" evidence="3">
        <location>
            <begin position="180"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="CDCP1 second and fifth CUB" evidence="4">
        <location>
            <begin position="368"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="CDCP1 third and sixth CUB" evidence="3">
        <location>
            <begin position="472"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="689"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="708"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="776" mass="86608" checksum="FC1482CE6A4B6592" modified="2018-12-05" version="1">MIEMATRCEGMLNVCKVTQKKHFSPCPLGGSILIHSCSEKIKQQNESIFCFSALSSDCSKTSCLGNTIQAQKEFFPNFNRTFTWDIKVESTQAFHLDFPGTGLRQIPNEGTCPDGHTYSVILYLHRAAPATIGTFCKGGTVTSVLGQFKGRVTLTVPSDVTLDPLDFQLKVGPKSEATAIVKVNLPRGVSDTVIITPNYPKDFPDDEQILWEFSIPGMHNYSVQFSEHSAPECLDEGEVVVVEYQKEGMKPTRTALTDPQPQHQQGGFNLTLKNCKTNQTLPGLSLRYTVSVIRSGHPGEIWEQSDLDHHVWSSLKSDEVSVQIEKVGSDPLCEMRKDSVETKTINIFFLLVAACADASLCTSTPLSVPTLDSCLPIPLHSFSWLFTIPHEATLDLVSPTGSLRQSLPGQECDPSSVLRVAENDGSFIGDFCSNGVIQKLQVHSNISITATPRDVRSNEPLSSFCMISISSSEAIIYRISPNLMFPTILATPNWPNGMKPHSSVSWVVDLPWMYHALIKFVISGLECEKGHARINMKHQEGGNDLLDIDESEKMRTENMTAHQSFYLNMTNCQPEGHLICFCFFSTDLLAILIGLVGAVLLLLLILCYISCFFSYRKKKSKMNSQNSIYMGKGNIFRPNDRHFVKTRADNDSHVYDYIDETMVYGHLLNDSESLPNGMQVDSYQTFTGPTDRTLPVVKEPDPEPEKGQYQSFLSPSDTFMPPRPRTPIERQDSLGFQDRRMMDNELYTFKSTGDMNTIRLSGVDMEPQPPIMEESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUD8</accession>
    <name>A0A3B5MUD8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LRRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MUD8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027373"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027706.1">
        <property type="protein sequence ID" value="ENSXCOP00000027373.1"/>
        <property type="gene ID" value="ENSXCOG00000020441.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156400"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050541">
        <property type="entry name" value="LRR_TM_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369">
        <property type="entry name" value="ANTIGEN BSP, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24369:SF213">
        <property type="entry name" value="INSULIN LIKE GROWTH FACTOR BINDING PROTEIN ACID LABILE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01463">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="LRRCT" evidence="4">
        <location>
            <begin position="178"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="LRRCT" evidence="4">
        <location>
            <begin position="363"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="464" mass="53191" checksum="0DF5AB15E443C0BA" modified="2018-12-05" version="1">MISTQNAIVVTGHRVISLIILFNGMNMKSDILVKYAGLFCVFFQRSRMKALRRQSRSGFSGIVLPNCSEMTQSGEIYLDCQDQSLTSIPMASTWSRVPNHLLLARNQIKALRSGSFLGYERLSSLDLQQNQISQVEEDAFQGLTHLTTLLLQHNQLRTLSEEALIPMPNLHYLRLYDNPWKCFCPMESLIRTLQVPSNRNLGNHAKCAEPVSLKDRKLKQINSDLLCEEFGKNVNVTQTVEPNPIRGKLDATALCHTYLFPVTWMDCRNRGLTEVPSGIPDNVVRVDLSLNKIHHLKPRDFQGAKSLRILNLSSNDMQRIDTGSLSGLLHLQELDLSSNQLHFVQYGVLEDLYFLSKLKLGGNPWMCDYNIHYMVYWLRLHPGVKYSSLLCQSPLQYIGERVEDYVHSYNRDCPKDRQLSKPDQNQRDAELWDSPMELQGAVEEEELEPSHLRAPQKYQIFRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUL6</accession>
    <name>A0A3B5MUL6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Sodium/myo-inositol cotransporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Sodium/myo-inositol transporter 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Solute carrier family 5 member 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="15">Interacts with KCNQ2 (via the pore module). Interacts with KCNQ1; this interaction is direct. Forms coregulatory complexes with ion channels KCNQ2-KCNQ3 and KCNQ1-KCNE2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Basolateral cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MUL6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027211"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027544.1">
        <property type="protein sequence ID" value="ENSXCOP00000027211.1"/>
        <property type="gene ID" value="ENSXCOG00000020322.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161679"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005412">
        <property type="term" value="F:D-glucose:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015798">
        <property type="term" value="P:myo-inositol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1730.10:FF:000013">
        <property type="entry name" value="sodium/myo-inositol cotransporter isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018212">
        <property type="entry name" value="Na/solute_symporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819:SF150">
        <property type="entry name" value="SODIUM_MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00456">
        <property type="entry name" value="NA_SOLUT_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00457">
        <property type="entry name" value="NA_SOLUT_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0915">Sodium</keyword>
    <keyword evidence="14" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="368"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="409"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="449"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="513"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="659"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00074169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00078598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="681" mass="74664" checksum="F7480B0B62D002AB" modified="2018-12-05" version="1">MATNATMEAADVVVIVIYFILVLGIGFAAMRKSNRGTVSGYFLAGRSMNWFAVGASLFVSNIGTEHFIGLAGSGAASGFSVAAWEFNALLLLQLLGWVFIPVYIHCQVYTMPEYLCKRFGGKRLKVYFAALTLVLYIFTKLSVDLYSGALFIQESLGWNLYVSIIVLIVMTAALTVTGGLVAVIYTDTVQAIMMIVGALCLTGIGFAKVGGLEEKYMQASPNITAILLSSPNLTYSESCKHHLHPKANALKILRGPKDPDLPWPGFLLGQTPASIWYWCADQVIVQRVLAAKNIVHAKGSTIMAGFLKTLPMFVMVMPGMISRVLFTDQLACIGPDHCMQVCGSAAGCSNVAYPRLVMSVMPVGLRGVMMAAMIAALMSDLDSIFNSASTIFTLDIYKMLRKDASPKELVITGRLFVILMVALSIAWVPVVIEMQGGQMFYYIQEVSDYLTPPVAVLFLLGVLWQRCNETGAFWGGVVGSALGALRLLLAVVYREPYCGQPDERPFFIKQIHFMYVAAILFWVSAITTVVVSLYTPPPEKQLIRTTTLWGLRQRMKSTDGRDENTQSANGNIIPARSSLQNDSYNLIAEPMLEGEGDGREVKDVEEGKECRLGEGGNSVKVCGGICGRRKKSSQSRVITAQEQERIINKLLYEPPRTRILLNTALVLICVIGIFLYLYFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVL4</accession>
    <name>A0A3B5MVL4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Runt-related transcription factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVL4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027621"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027956.1">
        <property type="protein sequence ID" value="ENSXCOP00000027621.1"/>
        <property type="gene ID" value="ENSXCOG00000020609.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159255"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002062">
        <property type="term" value="P:chondrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0051094">
        <property type="term" value="P:positive regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045595">
        <property type="term" value="P:regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.720:FF:000001">
        <property type="entry name" value="Runt-related transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.770.10">
        <property type="entry name" value="Signal Protein Aml-1b, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000040">
        <property type="entry name" value="AML1_Runt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012346">
        <property type="entry name" value="p53/RUNT-type_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013524">
        <property type="entry name" value="Runt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027384">
        <property type="entry name" value="Runx_central_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013711">
        <property type="entry name" value="RunxI_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016554">
        <property type="entry name" value="TF_Runt-rel_RUNX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11950">
        <property type="entry name" value="RUNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11950:SF40">
        <property type="entry name" value="RUNT-RELATED TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00853">
        <property type="entry name" value="Runt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08504">
        <property type="entry name" value="RunxI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009374">
        <property type="entry name" value="TF_Runt-rel_RUNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00967">
        <property type="entry name" value="ONCOGENEAML1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51062">
        <property type="entry name" value="RUNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Runt" evidence="8">
        <location>
            <begin position="65"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR009374-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="423" mass="45536" checksum="F48332CC84A97269" modified="2018-12-05" version="1">MSKFRRYDSCHHVHFLPDPAPGRRYTPPSTSLASGGKMAEALPLGAQEAGGGATVMGKFRIADHSMAEVISDHPGELVKTDSPNFLCSVLPTHWRCNKTLPIAFKVLTLGDIPDGTLVTVMAGNDENYSAELRNATAVIKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYQRAIKITVDGPREPRHSRQIQSSPSWSYDQSYPYLGQITTPTVHTTAPLSPGRSSLGDLSSRLTGPDLTAFGDPRMALERPFSSLPSLPDSRFSNPRVHYPPTGAAFTYTPTHNPVSNGAISITMATAMATTQTGRYHTYLPPPYPANTPHQTQNGPFQSTSSPYHLYYSATTGSYQFSMMAGGGGGSDSHSPPRILPPCTTASTGSSLLHPSLPNQNEGVGVEVESSHSSSPTNMVAAEAVWRPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MX05</accession>
    <name>A0A3B5MX05_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secretory carrier-associated membrane protein</fullName>
            <shortName evidence="5">Secretory carrier membrane protein</shortName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SCAMP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MX05"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024667.1">
        <property type="protein sequence ID" value="ENSXCOP00000024374.1"/>
        <property type="gene ID" value="ENSXCOG00000018202.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156476"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007273">
        <property type="entry name" value="SCAMP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10687:SF7">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10687">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN SCAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04144">
        <property type="entry name" value="SCAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="68"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="325" mass="36219" checksum="82330D1177D5FE69" modified="2018-12-05" version="1">MSGLDSNPFADPVDVNPFQVSARCSFVTFSRFESAGKFTGTTIPVSKASSSQPAVLPTSVEQSPQATAAAAQANLVRQQEELERKAAELERKEQELQNRSTKENNWPPLPRFFPIKPCFYQNFEEDIPEDYRRICKRMYYLWMFHSATLFLNILACLAYFTADAGKGVDFGLSILWFILFTPVSFVCWYRPVYKAFRSDSSFSFFFFFFVFFFQVAVYIIQSVGIPSWGNSGWISSISMIRTNLAVAVVMMVVAGFFTVNAVLGVILLKMVHSKYRNSGASFSKAQQEFSQGVLTNRTFQSAASSAATSAATSAAQGAFSNNQGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N037</accession>
    <name>A0A3B5N037_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATPase inhibitor, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">ATP synthase F1 subunit epsilon</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase. Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 8">Homodimer; represents the active form and is present at a pH value below 6.5. Homotetramer; represents the inactive form and is present at a pH value above 7.0.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">Forms an alpha-helical dimer with monomers associated via an antiparallel alpha-helical coiled coil, leaving each N-terminal inhibitory region accessible for interaction with an F1 catalytic domain. The inhibitory N-terminal region binds the alpha(ADP-bound)-beta(ADP-bound) (ATP5F1A-ATP5F1B) interface of F1-ATPase, and also contact the central gamma subunit (ATP5F1C). This dimeric state is favored by pH values below 7.0, and at higher values the dimers associate to form inactive homotetramer, where the inhibitory region is occluded, masking its inhibitory activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ATPase inhibitor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N037"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026340"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026659.1">
        <property type="protein sequence ID" value="ENSXCOP00000026340.1"/>
        <property type="gene ID" value="ENSXCOG00000019670.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165091"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042030">
        <property type="term" value="F:ATPase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030218">
        <property type="term" value="P:erythrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007634">
        <property type="term" value="P:optokinetic behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150077">
        <property type="term" value="P:regulation of neuroinflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000003">
        <property type="entry name" value="ATPase inhibitor B, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007648">
        <property type="entry name" value="ATPase_inhibitor_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48417">
        <property type="entry name" value="ATP SYNTHASE F1 SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48417:SF1">
        <property type="entry name" value="ATP SYNTHASE F1 SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04568">
        <property type="entry name" value="IATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64602">
        <property type="entry name" value="F1 ATPase inhibitor, IF1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="29"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="64"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00026043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="106" mass="11923" checksum="7375E2741D7C231D" modified="2018-12-05" version="1">MARLFLRGSLQRCIATQIRMSSDQLGELGKGAGKGGGGGGSVRSAGGAFGKREAAEEEQYFREKEQMEALRKHHGEEIEHHKKEIERLQREIDRHKGKIRKLSHDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N173</accession>
    <name>A0A3B5N173_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RNA binding motif protein 28</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N173"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027260"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027593.1">
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    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="RRM4_RBM28_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding motif protein 28"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
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    <dbReference type="InterPro" id="IPR051945">
        <property type="entry name" value="RRM_MRD1_RNA_proc_ribogen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48039">
        <property type="entry name" value="RNA-BINDING MOTIF PROTEIN 14B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48039:SF5">
        <property type="entry name" value="RNA-BINDING PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="54"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="200"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="303"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="405"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="435"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="461"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50102"/>
        </source>
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            <dbReference type="Ensembl" id="ENSXCOP00000027260.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="470" mass="53973" checksum="921D4857B389E65C" modified="2018-12-05" version="1">LQSSDEDDIEEEDDDDGDAEEEEDDEEQDEIKEEDNIQKQKPKKHLPSDVNEGRTVFIRNLSFDTEEEGLEEVLLQFGEVNYVKIVLHPETQHSKGQKNCQYTVLLTYCSFFFQNGGVRADGRKLMIVTAVSREDALKLKVDKKKVETGSRNLYLAREGLIRAGTKAAEGVSEADMAKRVRFEEIKRAKLRDISVFVSKNRLCIHNLPKSVDDKKLKALCLKGLQGTKGVRITECRVMYDRKPEKGRVTGQSLGYGFVQFRDHEQALATLRYLNNNPDIFGPHKRPIVEFSLEDSRKLKLKEMRKQRNKDFIQSRPVKREVKKSQTGSDTKITKKDGNKGQSSSHLAGKEKGQKQNFSGFHTKPEIEHVDLQNGKKRKKVLLFPSHRGPKIRMRDKDKQQVPPPKKARPGSKRNKGPTQPFEKSNQAKKQKQFKRKDGDRFDSLVEQYKRKLMGGGDKNASIKRSKWFDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N1U8</accession>
    <name>A0A3B5N1U8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase II subunit RPB3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">DNA-directed RNA polymerase II subunit C</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_028028079.1"/>
    </dbReference>
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    <dbReference type="STRING" id="32473.ENSXCOP00000027810"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028145.1">
        <property type="protein sequence ID" value="ENSXCOP00000027810.1"/>
        <property type="gene ID" value="ENSXCOG00000020734.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114151114"/>
    <dbReference type="KEGG" id="xco:114151114"/>
    <dbReference type="CTD" id="5432"/>
    <dbReference type="GeneTree" id="ENSGT00950000183100"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07031">
        <property type="entry name" value="RNAP_II_RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB3"/>
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    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000024">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
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    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
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        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001988">
        <property type="entry name" value="PRK00783.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
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    </dbReference>
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        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="10">
        <location>
            <begin position="18"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="275" mass="31450" checksum="220F5D7770BE1AE4" modified="2018-12-05" version="1">MPYANQPTVKITELTDENVKFVIENTDLAVANSLRRVFMSEVPTIAIDWIQIDANSSVLHDEFIAHRVGLIPLTSDDIVDKMQYSRDCTCDDFCPECSVELTLDVRCTEDQTRHVTSRDLLSNNPRVIPVTSRSRDNDPNDYVEQDDILLVKLRKGQELRLRAYAKKGFGKEHAKWNPTAGVSFEYDPDNALRHTVYPRPEEWPKSEYSEIEEDEVQAPYDPNGKPERFYFNVESCGSLRPETIVMSALAVLKKKLSDLQTQLSHEIQSDVLTIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRL8</accession>
    <name>A0A3B5KRL8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fork-head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRL8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001127"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001140.1">
        <property type="protein sequence ID" value="ENSXCOP00000001127.1"/>
        <property type="gene ID" value="ENSXCOG00000000927.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162993"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20036">
        <property type="entry name" value="FH_FOXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052328">
        <property type="entry name" value="FOX_transcription_regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46789">
        <property type="entry name" value="FORKHEAD BOX PROTEIN R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46789:SF2">
        <property type="entry name" value="FORKHEAD BOX PROTEIN R2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="6">
        <location>
            <begin position="146"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="5">
        <location>
            <begin position="146"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="280" mass="32259" checksum="C17307D606F9EE51" modified="2018-12-05" version="1">MTFQLKTRGRFCGLHCSVGLTDWDMDKELKLATTTDQNCNDKVNDQYLAQRPSARACRRKDEFIWYDKTSDTFVKPNLWLLVNPNIACPVRYVQTDADLQPEPGHADQLLQLEPGDAEEDSSKPVRTRRKGKITKVVSLKPGCWPRPPVNYCVLVALALKSSQTGSLKVQQIYNFTREHFPFFQTAPDGWKNTIRHNLCFSSSFRKTCNQLCKEGKRKSCFWHLTLDGHRRLQDELHTLTGETLKMLERSMSNPVVRTHTHTHTHVSLHRGLLICVSNHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTA3</accession>
    <name>A0A3B5KTA3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="RefSeq" id="XP_027870250.1">
        <property type="nucleotide sequence ID" value="XM_028014449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTA3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000846"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000856.1">
        <property type="protein sequence ID" value="ENSXCOP00000000846.1"/>
        <property type="gene ID" value="ENSXCOG00000000710.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114142891"/>
    <dbReference type="KEGG" id="xco:114142891"/>
    <dbReference type="CTD" id="8576"/>
    <dbReference type="GeneTree" id="ENSGT00550000075037"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13986">
        <property type="entry name" value="STKc_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052239">
        <property type="entry name" value="Ser/Thr-specific_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45998">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45998:SF2">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="20"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="305" mass="34432" checksum="97D3D24EDA896D1E" modified="2018-12-05" version="1">MGQALCICSRGSITIDNKKYYFVQKLDEGGFSYVDLVEGAKDGQFYALKRILCHDRQARQEAQTEVEMHQMFNHPNILSLVAYAFSDSGGKTEAWLLLPYVRKGTLWSVLEKLRDKGSSMPENQILTIFRGICSGLKTIHDKGYAHRDLKPTNVLLDEEDRPVLMDLGSMNRARIEVRGSREAMTVQDWAAQRCTISYRAPELFNVESHCIIDERTDIWSLGCVLYCMMMLEGPYDLIFQKGDSVALAVQNPVTIPSTCSYSNGLQMLLSSIMVSNPQERPGVGWVLDQVQDLQSRSPNTQTNMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTE4</accession>
    <name>A0A3B5KTE4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">OXA1L mitochondrial inner membrane protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the OXA1/ALB3/YidC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTE4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000886"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000898.1">
        <property type="protein sequence ID" value="ENSXCOP00000000886.1"/>
        <property type="gene ID" value="ENSXCOG00000000742.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063506"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20069">
        <property type="entry name" value="5TM_Oxa1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="YidC/Oxa/ALB_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03592">
        <property type="entry name" value="yidC_oxa1_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428:SF66">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEIN OXA1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="OXA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Membrane insertase YidC/Oxa/ALB C-terminal" evidence="12">
        <location>
            <begin position="114"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="377"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="377"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="402" mass="44220" checksum="B76EFE52376F2B75" modified="2018-12-05" version="1">GCSRTAQIRSQISCRHRGKLVWVSAVAVRQNSSQIPAEGVSAAASVADSSASLPADALAVLAQQAADAAPVAADILQAVAAEPRLAELGLAGYTPVGLIQNLLEFFHMDLGLPWWGAIVVGTVLARLAVFPVIVKGQREAAKLNNVLPEMTKLTEKMNEAKQSGNKYEFAKAYSDLNLFQKKHDVNPLRGFLVPLIQAPVFISFFIALRKMAYLPVPSLQTGGVLWFTDLSAADPFYILPFVVTGTMFFILELGAESGIDNPNLRTMKTVFRIMPLIILPLTINFPTAVFTYWLTSNCFSLGQVALLRHPAVRRMFNIPERVKHPAAELPQSDGLIESMKKGWKNAQLAHQLEERERRIKSHLDIAAKGPLRQTFTHNPLQQTPQGAKQQKIKAKPWKDMIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KUX2</accession>
    <name>A0A3B5KUX2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">VPS35 endosomal protein-sorting factor-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS35L family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KUX2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001805"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001832.1">
        <property type="protein sequence ID" value="ENSXCOP00000001805.1"/>
        <property type="gene ID" value="ENSXCOG00000001459.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011343"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029705">
        <property type="entry name" value="VPS35L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13673">
        <property type="entry name" value="ESOPHAGEAL CANCER ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13673:SF0">
        <property type="entry name" value="VPS35 ENDOSOMAL PROTEIN-SORTING FACTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="709" mass="80769" checksum="000D730119AE5A15" modified="2018-12-05" version="1">MCLDPRPLPDSFTVEDVNDTAKETCLNWFFKIASIRELLPRLYVEAAILKCNRFLSKSGIQETLPRLTAMIRGMGDPLVAAYARAYLCRVGMEVAPHLKESLNANVFDLLATFRQISGDSVQKQLHMQKVEVPAYLTLYSPAINWILQCVAYRAPEQLLTEMMERCKMMANNALLLNSIMRAFRPEFVATRATDFIGMIKDCDESGFPKHLLYGSLGRSLACADPPESERLTILNEAWKVITKVRNPQDYMNCAEIWVEFTCRHFTKREVNTVLADIIKHMTPDRAFEEAYPQLQSVIRKILTYFHDFSVLFSMERFLPFLDMFQKDSVRVEVCKSIMEVFIKHQLELTRDPVILNAMLHICKTMHDSVNALTLEDEKRSLALLIIGFIRMVSFGRDFEQQLSFCVEARATFCNLEPVLVQLIHTVNQLAMETRMVMKGNHSRKTAAFVRACAAYCFITIPSLSSIFSRLHLYLLSGQVALANQCLSQADAFLKAAVSVLPEVSRSISVEGKLRSSESFLLDYINNFLATLLVVPDHPEHGVLYLVRGLLNMVQDYTWEDNSDAKVRVYISALPLLAAMSQETYLYSIPKMDSNETLYGGDPKFLSEINKLCETLIGQILDHLKTLTRDESVRRQSALAFSLFGVLLAHGDLRNNKLSQLSINLWNLSHKHGHCETRIRTLESIRHQAQRPDMAHLSDTIQRLALQSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXH9</accession>
    <name>A0A3B5KXH9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Striatin interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the cortical actin filament dynamics and cell shape. Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the STRIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXH9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004808"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004866.1">
        <property type="protein sequence ID" value="ENSXCOP00000004808.1"/>
        <property type="gene ID" value="ENSXCOG00000003768.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00400000022095"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040185">
        <property type="entry name" value="Far11/STRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021819">
        <property type="entry name" value="Far11/STRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012486">
        <property type="entry name" value="Far11/STRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13239">
        <property type="entry name" value="PROTEIN REQUIRED FOR HYPHAL ANASTOMOSIS HAM-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13239:SF7">
        <property type="entry name" value="STRIATIN-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11882">
        <property type="entry name" value="DUF3402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07923">
        <property type="entry name" value="N1221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01293">
        <property type="entry name" value="DUF3402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01292">
        <property type="entry name" value="N1221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Far11/STRP N-terminal" evidence="7">
        <location>
            <begin position="14"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Far11/STRP C-terminal" evidence="8">
        <location>
            <begin position="410"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="285"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="327"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00046123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="787" mass="90515" checksum="9F8FC4E365823238" modified="2018-12-05" version="1">MQAAMCCSQGLSEFPDFEFEYSDTDKWAAELSELYSYTEGPEFALNRKCFEEEFRAHVSDKKWTDLDAAQHRAHAMRLLDGLEVIGREKRLKVARAILYMAQGAFADCSCEAEVQYWMRYNIFLLLDVGTFSALVELLNMEIDNSAACSSAVRKPAISLADSTDLRVLLNIMYLMVETIQQDDPADKPEWKIMKETFRAELGSPLFNNEPISVMLFGMVTKFCSGHAPHFPMKKVLLLLWKSILFTLGGFEQLQSIKVCKREELGLPPLPEDSIRVIRSMRAASPPASASDLIEQQQKRARREHKALIKQDNLDAFNEKDPYKADDSREDEDDNDDNDNSLEPETFPLERDEVMPPPIPHPPSERVSFPKGLPWAPKVREKDIENFLESSRSKFIGYTLGSDTDTVVGLPRPIHESIKTLKQHKYISVAEVQMSKEEEYQKTPLSVGEEEVEMCATELLYQGILPSLPQYMIALLKILLAAAPTSKAKTDSINILADVLPEEMPTTVLQSMKLGVDVNRHKEIIVKAISAILLLLLKHFKLNHIYQFEYMAQHLVFANCIPLILKFFNQNIMSYITAKNSISVLDFPYCVVHELPELTAESLEAGDNTQFCWRNLFSCINLLRILNKLTKWKHSRTMMLVVFKSAPILKRALKVKQAMMQLYVLKLLKVQTKYLGRQWRKSNMKTMSAIYQKVRHRLNDDWAYGNDLDARPWDFQAEECALRASIERFNSRRYDKSHSNPDFLPVDNCLQSVLGQRVDLPEDFQMNYDLWLEREVFSKPISWEELLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZ42</accession>
    <name>A0A3B5KZ42_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZ42"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005419.1">
        <property type="protein sequence ID" value="ENSXCOP00000005358.1"/>
        <property type="gene ID" value="ENSXCOG00000004191.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286924"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46484:SF7">
        <property type="entry name" value="MYELIN-ASSOCIATED GLYCOPROTEIN-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46484">
        <property type="entry name" value="SI:CH211-171H4.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="156"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="306" mass="34671" checksum="A793BB8B15428B84" modified="2018-12-05" version="1">QIPSVRLDDHCKFSTPFVLILCPTTEEQWKASVVKSIEALVDSCVVLPCTFTYPGSFLSTSRLRGIWHRKDKWNEIFYHEDTTSVLDNFKGRTKMLGNLGQNNCTLEIVQVKNHDIGPFCFRIEFVKKDTNQPTTEKFSFVEECAEIKMLEEPPQPELIQSNTAVQGKPYAVTCSVRHTCPSHVPVFTWSRGSEDDIIQIQKPIVSGIWETQSIMVFIPGENDDHTEITCSATFNGGRRSSTSIKLNVKRTGNIYHLIIPVAVAIGTAVIFGVFCIAVMKKYKNRIAELQSREGSMFNRLSRMSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZB9</accession>
    <name>A0A3B5KZB9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Replication factor A protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">PML body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the replication factor A protein 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027865422.1">
        <property type="nucleotide sequence ID" value="XM_028009621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZB9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000909"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000921.1">
        <property type="protein sequence ID" value="ENSXCOP00000000909.1"/>
        <property type="gene ID" value="ENSXCOG00000000107.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114139588"/>
    <dbReference type="CTD" id="6118"/>
    <dbReference type="GeneTree" id="ENSGT00390000010045"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04478">
        <property type="entry name" value="RPA2_DBD_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000168">
        <property type="entry name" value="Replication protein A 32 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000149">
        <property type="entry name" value="Replication protein A 32 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040260">
        <property type="entry name" value="RFA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014646">
        <property type="entry name" value="Rfa2/RPA32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014892">
        <property type="entry name" value="RPA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989">
        <property type="entry name" value="REPLICATION PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989:SF16">
        <property type="entry name" value="REPLICATION PROTEIN A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08784">
        <property type="entry name" value="RPA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036949">
        <property type="entry name" value="RPA32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Replication protein A C-terminal" evidence="13">
        <location>
            <begin position="163"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)" evidence="11">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)" evidence="11">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036949-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="278" mass="29929" checksum="B494828EAB58B962" modified="2018-12-05" version="1">MWNQGGYSESGMMGGYTQSPGGFASPSLSQGGEKKGRTRATQIIPCTVSQLMSASQVDEVFRVGDVEVTQVTIVGIIRSTDKSMTNIQYKVDDMTGAPMDVKQWVDTEDPSVDSTVLPPGTYVKVSGNLRSFQNHRSVVAFSVRTLEDMNEITSHILEVVHAHMALGKPQTMPAIGGGMTVNSMPMLQQGPGMTAGSYAGTNEIPLMVINGLSGNQNQVLSLIKGCPDPQGISIQDLKQRLNGISLTVIKQAVEFLSNEGHIFSTIDEDHYKSTDNDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZH8</accession>
    <name>A0A3B5KZH8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZH8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003957"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004001.1">
        <property type="protein sequence ID" value="ENSXCOP00000003957.1"/>
        <property type="gene ID" value="ENSXCOG00000003077.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157677"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072359">
        <property type="term" value="P:circulatory system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05871">
        <property type="entry name" value="Ig_Sema3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000015">
        <property type="entry name" value="Semaphorin 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000030">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000001">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF20">
        <property type="entry name" value="SEMAPHORIN-3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Sema" evidence="13">
        <location>
            <begin position="33"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="538"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="731"/>
            <end position="758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="766" mass="86388" checksum="29F8ABB7852086DD" modified="2018-12-05" version="1">MLGKTLHVMKCVPLSFNRDNKATNRVTPANFFSFSALSTDLLSTNRSSIFSGHDGQLSLSAVLLDEYRDRLFLGGKDVLYSLMLSPVSSESKEIYWPPLPGNREDCVQTGKDQTECANFVRLLQPYNRTHLLACGTGAFQPMCAFIYVGHRGEVSGGVEERCASVLCGELYTGLTADFLGRDSVIFRSMGGRTTMRTETDQKLLHDPRFIAAHIIPDNDDRDDDKVYFFFTEKASEGGDKEEAIHTRVGRVCVNDVGGQRVLVNKWSTFIKARLVCSVPGPHGIDTHFNQLEDVFLLRTKDERNPDIYAIFSTISNVFQGFAVCVYHMADIREAFNGPFAHKEGPNYQWGPYEGRMPYPRPGVCPSKITNQPGKEFGSTKDFPDSVLQFARSHPLMWRPVLPALRQPVLVKANIPYKLKQIVVDRVEAEDGQYDVMFIGTDVGTVLKVISLHGGNSLEAEEVTLEELQVFKKLFSYCVSPHSFSLASTQQALYVGSAAGVAQVKLHRCETYGKACAECCLARDPYCAWDGSSCARFVPNSKRRFRRQDVKHGNAVLQCVDQNVSDLDVTEDKVVYGTEKNSTFLECVPRSPQASVTWLVQRDDRKEEVKLDDRVMSTDQGLLFRHLFRQDEGVYICRSREHSFSQTLARISLQVLQADTVDELIARDIAGISDGFKDRSPGNHRPWMPCSTGPYSRGQIGQSRTWFKDIMQLIGPSNLPHVEEYCERMWCNDKLRRKHKSMLEKYRQAQESARKATSEILLHTLLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZL6</accession>
    <name>A0A3B5KZL6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBX domain-containing protein 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZL6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003071"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003108.1">
        <property type="protein sequence ID" value="ENSXCOP00000003071.1"/>
        <property type="gene ID" value="ENSXCOG00000002435.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018687"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02958">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14345">
        <property type="entry name" value="UBA_UBXD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01773">
        <property type="entry name" value="UBX_UBXN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000079">
        <property type="entry name" value="UBX domain-containing protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054109">
        <property type="entry name" value="UBA_8"/>
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        <property type="entry name" value="Ubiquitin-like_domsf"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
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    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 7"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="UCP037991_UBX7/2"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM00166">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="404"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="52"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="300"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="58"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="353"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="382"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR037991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="485" mass="54374" checksum="A571B76E2DB8E65D" modified="2018-12-05" version="1">MAALGDTSAPEANGLIQQFTAITGATESVGKHMLEACNNNLEMAVTMFLDGGGMAEEPSTSSSSAASSSRAPPTDEVRAPIPQKQDILVEPEPLFGVPKRRRPARSIFDGFRDFQTETIRQEQELRNGGTVDKKLSTLADLFRPPIELMHKGSFETAKDCGQLENKWLMINIQNVQDFACQCLNRDVWSNDAVKNIIREHFIFWQVYHDSEEGQRYIQFYKLNKFPYISILDPRTGQKMVEWNQLDVALFMEQATGFLAEHGQLDGPTCNAPPAKRARSESLIDASEDSQLEAAIRASLQETHYDSSNVHEAPDSPRSEDESDAEPFSDSEGPISVDCSDSETPAPHEEKSSTSKYTPSAMVTTAHQRLHPDSSTSSHRKSPYKENNHSHKKEESKKNHLEPSAPSPKARLMLRYPDGQREQISLSSKAKLLALVRHVQSKGYPNERFELVTNFPRRKLAHLDYDITLQEAGLCPQETVFVQERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L090</accession>
    <name>A0A3B5L090_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
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    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L090"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003291"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003329.1">
        <property type="protein sequence ID" value="ENSXCOP00000003291.1"/>
        <property type="gene ID" value="ENSXCOG00000002596.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183269"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="77" mass="8872" checksum="88E649DA8011BFBD" modified="2018-12-05" version="1">MNMTRKRSSPMLKRAGSDIMRANSNVRMPLAPLMRRRTRPIRASRITLKRVGDTKYFSMISLTGVRFGCEVLMLIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L0F7</accession>
    <name>A0A3B5L0F7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chemokine interleukin-8-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027864958.1">
        <property type="nucleotide sequence ID" value="XM_028009157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L0F7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004282"/>
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        <property type="gene ID" value="ENSXCOG00000003369.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114139301"/>
    <dbReference type="KEGG" id="xco:114139301"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0202">Cytokine</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017329071" description="Chemokine interleukin-8-like domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="4">
        <location>
            <begin position="26"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="113" mass="12365" checksum="E91AF9E37DD5A3A7" modified="2018-12-05" version="1">MSGIMKVFLLLAVAVCISKAQLNEPGQSCLCQRVRNGVFSKADIRDIQIYPASIFCDKVEIVVTSANGRYCLNPELQIVKKMLTRVLRRKAASTSKPTSLSNSSVSSRTTAQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L0Q4</accession>
    <name>A0A3B5L0Q4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">AATK</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L0Q4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004382"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004435.1">
        <property type="protein sequence ID" value="ENSXCOP00000004382.1"/>
        <property type="gene ID" value="ENSXCOG00000003458.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154244"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000347">
        <property type="entry name" value="Apoptosis associated tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000275">
        <property type="entry name" value="Apoptosis associated tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24417">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE LMTK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24417:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE LMTK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="112"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="804"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1065"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="828"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="849"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="878"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="923"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="968"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1181" mass="131652" checksum="8E738E935E13B505" modified="2018-12-05" version="1">MCTCVCVGVAEGCSGSHFSSDGTPQPSRSHLRDFYSDIRPVDASLCIFLFKEFENVDCDEYHADMSTLASTVSQGTPDVYVLPLTEVSLPTTKQPARSAQLLKSSELTRHSLLYLKEIGHGWFGKVLLGEVNTGLNTTQVVVKELKVSASVQDQMHFLEEAQPYRSLPNTALQWSNLVACRCIIFLCCVQGDVKGYLRSCRSNEAITPEPLILQRMACDIASGLLHLHKHNFTHSDLALRNCLLTADISVKIGDYGLAHTKYKDDFYVTSDQTYVPLRWIAPELVDEVHGNLLLADQTQQSNIWSLGVTIWELFELGNQPYRHYSDRQVLTYAVREQQLRLPKPLLKVPLAERWYEVMQFCWLQPDQRPNAEEVHLLLSYLCAKGASEAEEDFERRWNSLRPNAGFNGHHTASTISRDQPSLTSSSFPLLEQFSSGDGYHSESGDDILTVTETSHGLNFEYKWEQARAESSYISQESSNYQGSSGSFLTAISLTMEPLLGQRHTCNTWPSKHRHSSGGHHRRASHSCRQDSYTDFNYTMPSTDIEDTWPDQHSLAFHSLPKPIDYLEPHQAKDNNACLSLSKHHTMVPSDNCNAYIYLCHEGETPETPSGECCHTHFVDPLTGLLVRNNDYCHSYSHSSYITDKTTDILSSEEMINLSPAPGGPVVSKHTLIKPDDCEEHYINPSSEETALTGKREEVIKENLIMQNPPEPKKEEVTLTMTKNTPPPPDNRHVMVSITDPQLELSHIADSGMDQAGSTVSLIDILDCSDEEEDITDDITDVTSGIFADEGNELNVSPAFKSLQKQVGTPDSMDSMDLPSAAGSCEGLSPASSHPSSSPKAMDSGYDTENNEKETEDHILLPLSDKTPYRDSAYFSDYENEKQSRDEERERQLKEMDHHSDVEKQHVGGKKAEKRKSGEDEELKDGVVNKDLKLDVDHNPKGRAESLPPGTSLTENEEPSDSGRSSPADGEEADEEDADTDDSDDSDEELRSYSAQEEEEESEEECHPVPIVVSDNSEAHKLRSLLKMPSLAAENLEEDHKKKTVSFFDDVTVYLFDQESPTKELVEHGFPLASEGSSSGRKSQERPNISDDSSDGNISEERFEWEDDFPLLPLPTSSTAADSPPPHAVAKAPEPKPAIQLSRFTVSPSNVSRFSITHISDPDMDSAGGWSLRAFRVCIFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L0W5</accession>
    <name>A0A3B5L0W5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L0W5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005963"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006029.1">
        <property type="protein sequence ID" value="ENSXCOP00000005963.1"/>
        <property type="gene ID" value="ENSXCOG00000004625.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155439"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050970">
        <property type="entry name" value="Cl_channel_volt-gated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45720:SF6">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="109"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="219" mass="24217" checksum="7D89CE7B211E3F3C" modified="2018-12-05" version="1">MSLGEERLSKCACKTKKGIEGRSAEKGKTADLFAPVGVELDSTRLFLNGPRGLFLPHSLTLHEYTTSLLCRCYCSNFVVYIHLLALPMCVSTAQKWMYGGLDSNVFLQYLVWVTCPMVLITFSAGFTQVLAPQAVGSGIPEMKTILRGVVLKEFLTFKMFVAKIIGLICALGSGMPLGKEVTHTHTHPPPHLQTQQHQYSSPTLPYCLHQFVIRTQPGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1J6</accession>
    <name>A0A3B5L1J6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">KASH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus outer membrane</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the nesprin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1J6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004210"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004256.1">
        <property type="protein sequence ID" value="ENSXCOP00000004210.1"/>
        <property type="gene ID" value="ENSXCOG00000003308.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154481"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00176">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000126">
        <property type="entry name" value="Spectrin repeat containing, nuclear envelope 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012315">
        <property type="entry name" value="KASH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002017">
        <property type="entry name" value="Spectrin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14514:SF3">
        <property type="entry name" value="NESPRIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14514">
        <property type="entry name" value="PKA ANCHORING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10541">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00435">
        <property type="entry name" value="Spectrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01249">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51049">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="9">
        <location>
            <begin position="1"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="421"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="topological domain" description="Perinuclear space" evidence="9">
        <location>
            <begin position="445"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="KASH" evidence="12">
        <location>
            <begin position="415"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="348"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="355"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="473" mass="53498" checksum="1C6D231D9903D6BD" modified="2018-12-05" version="1">LIVSTGEAASADSHMHPLDVSRSHLQQLDSSRSRTPLSTETDASYMGYMRLLGECRGSIDTVKRMGSELKEEEDAVSGLADPSSSESQTSGVIERWELLQAQDLSKEMRSKQNQQQWQQLNSDLNNIWTWLGETEEELEQQQKLDVSTDIQIIKLRIKKLKELQKAYDKRKAIVLSINLCSAEFLQTDTEESRELQAKLKDMNNSWDKLGSSLDEWRTSLQEALMQCQDFHEMSHGLLLWLENIDRRRNEIVPIDPIQDSDTLHEHHKTLTQIRQELLDSQLKVAPLQDMSLQLLVNSQGSDCLEAKEKVHVIGNRLKLLLKEVTRDLRELSRTLDITSSQQDLSSWSSADELDTSGSLSPVSGRSTPSRSRSQRGKCSLSQPGPSVSSPHHRSRSAAGSASCRSDLDLATARRSSFWRRVLWFALPLQLLLLLLVVLAFLLPLAEEDYCTQSNNFAHSFYPMLSYTKGPPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1Q9</accession>
    <name>A0A3B5L1Q9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 3 family. TAS1R subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1Q9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004275"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004322.1">
        <property type="protein sequence ID" value="ENSXCOP00000004275.1"/>
        <property type="gene ID" value="ENSXCOG00000003362.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166394"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050909">
        <property type="term" value="P:sensory perception of taste"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000004">
        <property type="entry name" value="Taste receptor type 1 member 3-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF3">
        <property type="entry name" value="TASTE RECEPTOR TYPE 1 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00592">
        <property type="entry name" value="CASENSINGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017369494" description="G-protein coupled receptors family 3 profile domain-containing protein" evidence="13">
        <location>
            <begin position="20"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="563"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="599"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="632"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="676"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="727"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="756"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="784"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="14">
        <location>
            <begin position="561"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="834" mass="92183" checksum="CB992B304106EBB4" modified="2018-12-05" version="1">MFLTVVLSLGWLMLQLAAGELDYTSQAEGMRLQGDFSFAGLFPLHYTDVSANGLPALVPCDQGRPNKHGFHLMQAMRFAVEEINNSSGPLHLLPGVKLGYQMYDICSNPASFLATLDLLEQQYQNFSNTQRAVGVIGPDSSSKSFTPAALLGAYVIPQISYEASNEMLSNKEIYPSFFRTIPSDKNQVAAMIQLLVRFNWMWIALLGSDNDYGLQGMQSLSEQAPHHGICIPYQGIIPTASKDTVQTMRNMVDNILKTKVNTIVVFSSKTKFHDFLPYVLERNITEKVWIGTEDWSPSTLISGIPGIQTIGTVLGISVKDAAIPGFDEFQKNTVEISMQRVNGEANVTLGSGNDCLQSTDLYSLARNDFSMDKYDLTSSFNVYKAVYALAQALHQALGCDSAECSRRSVTPPELLAQLRKVRFSLGNSSVYFDMHGDPPTGYDIVSWVWRGTDWSLRVVGSFAPDPIALTVDASLIEWHGKEDSESVPESFCSPPCPKGHKKLLTGQHACCFDCQACPSATFLNKSDLTDCQACLPEQWAPPSSEECLNRTVIQLDWDHPLSIALLFFLATCLLLTSSTALILLLNLNTPVAKSAGGRTCLLMLAALTVAALSTLCHFGRPSPAACVLKQPLFTISFSVCLACIAVRSLQVVCIFKFASKLPLAYDRWTKKQGPEVTIFLVSATILLISVLRVSVDTPQPSQDLRFYQDKIVRECSKTLSVGSGIELAYVSVLSVLCFSFSYMGKDLPANYNEAKCVTFSLMVYMISWMSFFTLYLINRETFTMAAQVFATLFSVLAFLAGYFLPKMYIIILRPQMNTTAHFQNCIQMYTMNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L222</accession>
    <name>A0A3B5L222_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Forms serotonin (5-hydroxytryptamine/5-HT3)-activated cation-selective channel complexes, which when activated cause fast, depolarizing responses in neurons.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Postsynaptic cell membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L222"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004390"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004443.1">
        <property type="protein sequence ID" value="ENSXCOP00000004390.1"/>
        <property type="gene ID" value="ENSXCOG00000003467.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164924"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19063">
        <property type="entry name" value="LGIC_TM_5-HT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000017">
        <property type="entry name" value="5-hydroxytryptamine receptor 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008132">
        <property type="entry name" value="5HT3_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049944">
        <property type="entry name" value="LGIC_TM_5-HT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01708">
        <property type="entry name" value="5HT3RECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 20" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="278"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="307"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="342"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="21">
        <location>
            <begin position="45"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="22">
        <location>
            <begin position="250"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
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            <dbReference type="ARBA" id="ARBA00037540"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="438" mass="50056" checksum="12406F2BD03D942A" modified="2018-12-05" version="1">KLNILLSGISNLLCYKISLSFKVNCSKPDQPSLLAALSPVFDLMAIRPVMNQTTHTTITMYFTLYGILGVVCSVHSAYYYHRSFLFLQWWTSEFSRWDPVQCGTNNISIPKEKIWLPDIVINEFMEENKAPSIPYMYLFSDGRVHEALPLRVVSSCNLDIYAFPFDIQNCSLTFNSYIYYAIELKIILGRDAELMTVFSKDVMTTMGEWTLVDITAQKIEINDKSGLYPDMLAFYIRVQRRSIMYVVTLLLPSCFLITLDLFSFLLPPESTDRSSFKMTIIFGYTVFLLMMNDLLPITGNTIPLINVFFSLCLVLMVASLLETIFITNILINSSDFSPVPRWIRILVLNVLGCLVCMLRRREKPEGSGTEALMELKSLSLALQAIRCEVEQQHKGKQSSEEWIQVGLIIDRLLFGLYIAFISVSFITIIILWVNSCSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L3X0</accession>
    <name>A0A3B5L3X0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">5'-nucleotidase, cytosolic IAa</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="32473.ENSXCOP00000004536"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="5-nucleotidase"/>
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    <dbReference type="PANTHER" id="PTHR31367:SF4">
        <property type="entry name" value="5'-NUCLEOTIDASE, CYTOSOLIC IAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31367">
        <property type="entry name" value="CYTOSOLIC 5'-NUCLEOTIDASE 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06189">
        <property type="entry name" value="5-nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="339" mass="38321" checksum="02C9A5BA20C8516F" modified="2025-10-08" version="2">MQLILKFTPLSTQISVFLQPKPENAVTVAVSSRVLFRTEREQKVFEQKGVEEYLRYQIEHENEPFAPGPAFPFVKALDAVNTRLRELYPQSEELFDIVLVTYNHAHVGIRLINTINHHNLFIERFCMTGGSSPIGYLKAWHTNLYLSADAEKVREALAEGIAAATMFMPEKLTEVSESQLRVAFDGDAVLFSDESERIFKTHGLDKFFEHEKENEDRLLDHGPLKGFLEALGKLQKKFYAKGQRLDCPIRTYLVTARSAASSGIRALKTLRAWGLEIDEALFLAGAPKGPMLEKIRPHIYFDDQMFHVEGAAEMGTVAAHVPYGIAQKYNSQQKMSSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4M7</accession>
    <name>A0A3B5L4M7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">SRY-box transcription factor 19b</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027894376.1">
        <property type="nucleotide sequence ID" value="XM_028038575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L4M7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004791"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004849.1">
        <property type="protein sequence ID" value="ENSXCOP00000004791.1"/>
        <property type="gene ID" value="ENSXCOG00000003764.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114157522"/>
    <dbReference type="KEGG" id="xco:114157522"/>
    <dbReference type="CTD" id="64812"/>
    <dbReference type="GeneTree" id="ENSGT00940000165992"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01388">
        <property type="entry name" value="HMG-box_SoxB"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="transcription factor Sox-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
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        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
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        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF283">
        <property type="entry name" value="TRANSCRIPTION FACTOR SOX-19A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SOXp"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="8">
        <location>
            <begin position="56"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="6">
        <location>
            <begin position="56"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="304" mass="33399" checksum="F9CF4C91C0A5EF13" modified="2018-12-05" version="1">MYSLMEHELKPNGQPQTHQTTGMSPITGNLTGSMGTNGNSLPKTACPPGGDPMDKVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLTDAEKRPFIDEAKRLRAVHMKEYPDYKYKPRRKTKPLLKKDAQVGKYPLSAGNLLTAAAQNQGGSPRMEGYGWAPTGGYTGMQNEALSYTQQLHRYDLSALQYPPAMTAAQTYMSGANSYSPMSYSSSPQQLSPVNMPMVKAEPVAHSPSTGTPNHHRGALQGDLRDMISMYIPGPGGDSSDPATAQRGYTSVQQHYLSGTVPLTHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L598</accession>
    <name>A0A3B5L598_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">WD repeat and FYVE domain containing 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Early endosome</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_028045163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L598"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005987"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006055.1">
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        <property type="gene ID" value="ENSXCOG00000004647.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114161680"/>
    <dbReference type="KEGG" id="xco:114161680"/>
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    <dbReference type="GeneTree" id="ENSGT00940000157731"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034141">
        <property type="term" value="P:positive regulation of toll-like receptor 3 signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034145">
        <property type="term" value="P:positive regulation of toll-like receptor 4 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="WD repeat and FYVE domain-containing protein 1"/>
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    </dbReference>
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        <property type="entry name" value="WD repeat and FYVE domain-containing protein 2"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042234">
        <property type="entry name" value="WDFY1/WDFY2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
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        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46189">
        <property type="entry name" value="LD41958P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46189:SF2">
        <property type="entry name" value="WD REPEAT AND FYVE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
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        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
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        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0853">WD repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="20"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="195"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="238"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="10">
        <location>
            <begin position="281"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="362"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="410" mass="46537" checksum="ECA0CEFAF035F5F8" modified="2018-12-05" version="1">MAAEIHSRPQTARPVLLNKIEGHSDAVNAAVLIPKEDGVITVSEDRTIRVWLKRDSGQYWPSIYHTVSSPCSCMSYHHDSRRIFIGQDNGAVVEFLISEDFNKMNQVKTYPAHQNRVSSMVFSLESEWVVSTGHDKSVSWMCTQSGSMLGRHYFTSWASCLQYDYETQHAFIGDYSGQITLLKLEKQTYSTITTLKGHEGSIGSLWWDPVQRLLFSGASDHSIIMWDIGGRKGRTLLLQGHHERVQALRYLQFTRQLLSCSADGGIAVWNMDTQREEAPQWLDSDSCQKCEQPFFWNIKQMWDTKTLGLRQHHCRKCGKAVCGKCSSKRSTFPIMGFEFSVRVCDACFDTIKEEDRTPLATFHEGKHNIAHMDMDPSRGLMVTCGSDRIVKIWDMTQVVGSSLATGFSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5T5</accession>
    <name>A0A3B5L5T5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipoxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5T5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005765"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005831.1">
        <property type="protein sequence ID" value="ENSXCOP00000005765.1"/>
        <property type="gene ID" value="ENSXCOG00000004487.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166257"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034440">
        <property type="term" value="P:lipid oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.245.10">
        <property type="entry name" value="Lipoxygenase-1, Domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000907">
        <property type="entry name" value="LipOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013819">
        <property type="entry name" value="LipOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036226">
        <property type="entry name" value="LipOase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11771">
        <property type="entry name" value="LIPOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00305">
        <property type="entry name" value="Lipoxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48484">
        <property type="entry name" value="Lipoxigenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51393">
        <property type="entry name" value="LIPOXYGENASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoxygenase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="133" mass="14844" checksum="2EB35FB41F524137" modified="2018-12-05" version="1">MVIFRVSAQHAALNDGQFDYNSWIPNGSLLLCEPPPTTKGQSSMQTLLEVLPNVGDTVKIVSAVWTLSDKYTDVVPLGTYPEEHFDEPALKQMIKEFQAELSYLSEEITARNSQLKIPYPYLNPAVIDNSVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6A7</accession>
    <name>A0A3B5L6A7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the bacterial ribosomal protein bL36 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6A7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003309"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003347.1">
        <property type="protein sequence ID" value="ENSXCOP00000003309.1"/>
        <property type="gene ID" value="ENSXCOG00000002609.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010866"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00251">
        <property type="entry name" value="Ribosomal_bL36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052143">
        <property type="entry name" value="Mitoribosomal_bL36m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000473">
        <property type="entry name" value="Ribosomal_bL36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035977">
        <property type="entry name" value="Ribosomal_bL36_sp"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01022">
        <property type="entry name" value="rpmJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46909">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L36, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46909:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL36M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00444">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57840">
        <property type="entry name" value="Ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00828">
        <property type="entry name" value="RIBOSOMAL_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="95" mass="10239" checksum="8B1342747B2E855B" modified="2018-12-05" version="1">MGLTTASGVLPGATRANYISLTTPAPPNLITPLPSSVLPVQGAPSLLRLPGAHPSAGMKTKSALKKRCKDCFFVRRRGRLFVFCKTNPRHKQRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L732</accession>
    <name>A0A3B5L732_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Protein phosphatase 1B</fullName>
            <ecNumber evidence="6">3.1.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Protein phosphatase 2C isoform beta</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="21">Monomer. Interacts with PAK6. Interacts with the phosphorylated form of IKBKB/IKKB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 24">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L732"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008113"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008211.1">
        <property type="protein sequence ID" value="ENSXCOP00000008113.1"/>
        <property type="gene ID" value="ENSXCOG00000006227.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164695"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.430:FF:000001">
        <property type="entry name" value="protein phosphatase 1B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.40.10:FF:000001">
        <property type="entry name" value="protein phosphatase 1B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.430">
        <property type="entry name" value="Phosphatase 2C, C-terminal domain suprefamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012911">
        <property type="entry name" value="PP2C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036580">
        <property type="entry name" value="PP2C_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07830">
        <property type="entry name" value="PP2C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81601">
        <property type="entry name" value="Protein serine/threonine phosphatase 2C, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 24" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="18" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0519">Myristate</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15 24" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="26">
        <location>
            <begin position="76"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00065584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00070216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00082952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="431" mass="47405" checksum="13F5D027290A7782" modified="2018-12-05" version="1">MRTARKGNVEIPAFVRQLVKETEKRVSSFFKGGRGGASDGEQTGEGEKEEVIPSPYLDRPVLEKLTEEGCAHWGLTYALGRMQGWRANMEDFHNCVPQLGGELAEWSFFAVFDGHAGNTVAQYCSQHLLGHILTTGKKFHTEHVMKTCTVGAIVEGFMHTDKHLHSVARREGWERGGTTVVSTLISPYYIYFGNCGDSRAVLCRSGQVCFSTEDHKPYSPLEKERIESAGGSVSLQRINGSLAVSRALGDFSYKGVENRTPSQQMVSPEPEVCVVERSPMDEFLVLACDGVWDTISNEELCAFVHNRLQVCTDLRDVCTQVIDLCLYKGSLDNISIILLCFPGAPQLSAEALHQEAELEDLLESKVAEIYDELSASGEEPDLLSVLTVLASTVIPGLPPGGGIQSKRNCIISAYYHQRETHKPAVPNVSPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8I5</accession>
    <name>A0A3B5L8I5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Claudin 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CLDN8</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8I5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007132"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007224.1">
        <property type="protein sequence ID" value="ENSXCOP00000007132.1"/>
        <property type="gene ID" value="ENSXCOG00000005511.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159077"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="78"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="285" mass="31294" checksum="0F72ACCF076D4D75" modified="2018-12-05" version="1">MTQGFSEITAICTGLIGLIGASATTGSPMRKVTVSSGENKTDMERRWGLWMNCYSQANVRMQCKVYDSLLHLSPDLQAGRGLMCCSVALSGLGLLFALAGMKCIPWFQNIKWVKNTILMVAGGIQFMACICIFIPVSWTGHVILRDLYNPPLTDTQKREMGGAPYIGWVTGTFLFALALLFTCKCFPSDNKPTMTSYHPVSTLKSTRNSHNSKILDVQDPISLHNVAPEVTNREALVIQSTSAFLQSQSSLVPRASPPSTSFSLKCIPLLLFHYFPLISDATLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L941</accession>
    <name>A0A3B5L941_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L941"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004324"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004371.1">
        <property type="protein sequence ID" value="ENSXCOP00000004324.1"/>
        <property type="gene ID" value="ENSXCOG00000003406.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159561"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="156" mass="17713" checksum="744B6531A21FF6F4" modified="2018-12-05" version="1">RESDAAVALCERCVSIFLCSNVLSTARLGCKLDLNFIARNKWNCEYNPKVNELQIIRKPRSTARLFRNGTLVCAGAKSIDESHRAARKFARIVQKLNFPVRLLNFQIKNILSCGKFFPVHLADVRQAYNKECRSGQLFMSSNVQCLHSHVTLQVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAK3</accession>
    <name>A0A3B5LAK3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LIM/homeobox protein Lhx1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Homeobox protein Lim-1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027886308.1">
        <property type="nucleotide sequence ID" value="XM_028030507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAK3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007867"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007965.1">
        <property type="protein sequence ID" value="ENSXCOP00000007867.1"/>
        <property type="gene ID" value="ENSXCOG00000006042.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114152579"/>
    <dbReference type="KEGG" id="xco:114152579"/>
    <dbReference type="GeneTree" id="ENSGT00940000164085"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09367">
        <property type="entry name" value="LIM1_Lhx1_Lhx5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09375">
        <property type="entry name" value="LIM2_Lhx1_Lhx5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000006">
        <property type="entry name" value="LIM homeobox transcription factor 1-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000075">
        <property type="entry name" value="LIM/homeobox protein Lhx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000046">
        <property type="entry name" value="LIM/homeobox protein Lhx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049618">
        <property type="entry name" value="Lhx1/5_LIM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049619">
        <property type="entry name" value="Lhx1/5_LIM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050453">
        <property type="entry name" value="LIM_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208">
        <property type="entry name" value="LIM/HOMEOBOX PROTEIN LHX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24208:SF106">
        <property type="entry name" value="LIM_HOMEOBOX PROTEIN LHX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 11 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 11 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 12" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="15">
        <location>
            <begin position="62"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="175"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="177"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="135"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="344"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="148"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="362"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="381"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="407" mass="45178" checksum="39530423F69DB382" modified="2018-12-05" version="1">MLHCADCEKPILDRFLLKVLDRPWHIKCVQCCECKCSLTEKCFSRDGRLYCKNDFFRRFGTKCGGCSQGILPSDLVRRAKSKVFHLNCFTCMMCNKQLSTGEELYILDEFKFVCKEDYQNNNGKDTILLSVTTCSDPSLSPDSQDPQDDGKDSEGGHASDKEACGNDNDEQSAVGKRRGPRTTIKAKQLETLKAAFAATPKPTRHIREQLSRETGLNMRVIQVWFQNRRSKERRMKQLSALSARRHVFFRSPRRMRSLGERLEPGELGHFSYYGDYPSEYYGSGGNYEYFQGPPSSQAQTPADLGFVPSTVPAGTPLGAMDHHHHPHQHAGVGQHHCGAEVQCFSDGASHHPADSPSPEPSGPGSIHSISSEMCGPGTPFTAVSLSDNGYTNQLSQPSSELSEGTAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LC82</accession>
    <name>A0A3B5LC82_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Apolipoprotein Eb</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the apolipoprotein A1/A4/E family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027868052.1">
        <property type="nucleotide sequence ID" value="XM_028012251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LC82"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009783"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009901.1">
        <property type="protein sequence ID" value="ENSXCOP00000009783.1"/>
        <property type="gene ID" value="ENSXCOG00000007430.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114141610"/>
    <dbReference type="KEGG" id="xco:114141610"/>
    <dbReference type="CTD" id="30314"/>
    <dbReference type="GeneTree" id="ENSGT00950000182929"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042627">
        <property type="term" value="C:chylomicron"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903561">
        <property type="term" value="C:extracellular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034364">
        <property type="term" value="C:high-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034362">
        <property type="term" value="C:low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034361">
        <property type="term" value="C:very-low-density lipoprotein particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120020">
        <property type="term" value="F:cholesterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060228">
        <property type="term" value="F:phosphatidylcholine-sterol O-acyltransferase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055090">
        <property type="term" value="P:acylglycerol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033344">
        <property type="term" value="P:cholesterol efflux"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042157">
        <property type="term" value="P:lipoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033700">
        <property type="term" value="P:phospholipid efflux"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.20:FF:000010">
        <property type="entry name" value="Apolipoprotein E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000074">
        <property type="entry name" value="ApoA_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050163">
        <property type="entry name" value="Apolipoprotein_A1/A4/E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18976">
        <property type="entry name" value="APOLIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18976:SF2">
        <property type="entry name" value="APOLIPOPROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01442">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58113">
        <property type="entry name" value="Apolipoprotein A-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 12" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017395287" description="Apolipoprotein Eb" evidence="12">
        <location>
            <begin position="19"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="30091" checksum="3B7ECE7CF96C55C1" modified="2018-12-05" version="1">MKAVALILALAVITGCNGRVVRQAETSLTRVENSVERFLQYITDLNQQADGVLENLKVPELSKELETLITDTMSELTTYRDGIQSRLATYTAGSPDQIPEDLQLLANRLQKDMTDAKERSTEYLNELKTMVEQNTDDVRGRITAYTTKLRKRLTKDTEDIRDTVSTYMSEIQSRTNQNLDTVKENVEPYIEQARDSAAQKLKDISTILETQAETIRTQLESSMKELSTSMDSKLEELTELLSPYATQVREHLDNVMQKVKETATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LD73</accession>
    <name>A0A3B5LD73_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Si:ch211-108p6.4</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LD73"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005885.1">
        <property type="protein sequence ID" value="ENSXCOP00000005819.1"/>
        <property type="gene ID" value="ENSXCOG00000004519.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165285"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050380">
        <property type="entry name" value="Immune_Resp_Modulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23411">
        <property type="entry name" value="TAPASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="144"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="255"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="387" mass="43615" checksum="A362C2F47AF2550B" modified="2025-10-08" version="2">MPCQFTDEHVSLNNEGHIETQLIHRQAMLQFGQKGDTPVNPDAITFLITGSKLDLRRYLDGVEAEQLECELRRYSTEGIHIHWPSQEAKDYNRWFTCTLKQDKGLFTVTGFLRHPSNEPPPGQQDYRSWPAIADTEILTTSGKPQQIRNTTPSVKATLGSQQRLHCQFGVDHRGANITVEWHWQQRGERTKLFGHSSRTGQIQGSGVGKKALAGGDATYTLPFTKMSSEGTYICSVLVNPLFGNQDVVLHIEEPPRVSLNVGNVLSVLDGAEQKVVCEAENYYPLDVEIKWYEQDPAALGQRVGAPLSKMLQNILLSSHKHNQDMTYSLSGFFYLKASLEASGRQFTCIVSHKSLRMPIKKSFILQVEDKTKQNKKKNSFRVAKDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDV5</accession>
    <name>A0A3B5LDV5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BCAS3 microtubule associated cell migration factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDV5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007866"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007964.1">
        <property type="protein sequence ID" value="ENSXCOP00000007866.1"/>
        <property type="gene ID" value="ENSXCOG00000006041.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000170057"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="54" mass="5934" checksum="6667B341B979E06E" modified="2018-12-05" version="1">MNDKRMASDSPRRPSRCAGGVLVRAQAATEQSYMESVVTFLQDVVPQVTVSHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LF33</accession>
    <name>A0A3B5LF33_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Olfactomedin 3a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Synapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LF33"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006469"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006549.1">
        <property type="protein sequence ID" value="ENSXCOP00000006469.1"/>
        <property type="gene ID" value="ENSXCOG00000005009.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022082">
        <property type="entry name" value="Noelin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003112">
        <property type="entry name" value="Olfac-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050605">
        <property type="entry name" value="Olfactomedin-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192:SF90">
        <property type="entry name" value="NOELIN-3 PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23192">
        <property type="entry name" value="OLFACTOMEDIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12308">
        <property type="entry name" value="Noelin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02191">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00284">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51132">
        <property type="entry name" value="OLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="Olfactomedin-like" evidence="11">
        <location>
            <begin position="187"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="48"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="188"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51132"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="447" mass="51637" checksum="66B0FBD71CE5864F" modified="2018-12-05" version="1">MVSECCCVCFHLQTIGPKEGWQVYSSAQDADGRCICTVVAPEQSLCSRDAKSRQLRQLLEKVQNMSQSIEVLNLRTQRDFQYVIKMENQMKGLRTKFRQVEDDRKSIMAKNFQELKDKMDELKPLIPVLEQYKMDAALISQFKEEIRNLSVVLTGIQEEIGAYDYDELYQRVLRLDTRLRSCMGKLTCGKLMKITGPVTIKTSGTRFGAWMTDPLATTRNNRVWYMDSYTNSKIVREYKTIDDFVSGVVSQTYSLPFKWEGTNHIVYNGSLYYNKYQSNIIVKYNFETGTVLAQRALEFAGFHNMYPYTWGGYSDIDVMADELGMWAVYATNQNAGNVVISQIDPDTLQVLKTWNTEYSKRNAGESFMICGTLYITNSHLSGAKVYYAYSTKTSSYEYIDIPFHNQYFHISMLDYNAKERALYAWNNGHQVIFNVTLFHVIKTEDDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFB3</accession>
    <name>A0A3B5LFB3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PI3K-ABD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFB3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010848"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010973.1">
        <property type="protein sequence ID" value="ENSXCOP00000010848.1"/>
        <property type="gene ID" value="ENSXCOG00000008194.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155531"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000055">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PI3K_ABD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02192">
        <property type="entry name" value="PI3K_p85B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00143">
        <property type="entry name" value="PI3K_p85B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51544">
        <property type="entry name" value="PI3K_ABD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PI3K-ABD" evidence="2">
        <location>
            <begin position="17"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="128" mass="15138" checksum="F62A855ACFC6BA17" modified="2018-12-05" version="1">LCFGFIGRFLIQKPIFLFKQILVDCLLPNGMILTLECLREATLITIKHELFKEARKYPLHHLLQEETSYIFVSVTQEAEREEFYDETRRLCDLRLFQPFLKVIEPVGNREEKILNREIGKTGRKGSHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFM6</accession>
    <name>A0A3B5LFM6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1. Prevents the transactivation of STAT3 target genes. May play a role in CARD15-mediated innate mucosal responses and serve to regulate intestinal epithelial cell responses to microbes.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Complex I functions in the transfer of electrons from NADH to the respiratory chain. Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Complex I is composed of 45 different subunits. Interacts with CARD15, but not with CARD4. Interacts with STAT3, but not with STAT1, STAT2 and STAT5A. Interacts with OLFM4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Mitochondrion inner membrane</location>
            <topology evidence="1 14">Single-pass membrane protein</topology>
            <orientation evidence="1 14">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the complex I NDUFA13 subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027877124.1">
        <property type="nucleotide sequence ID" value="XM_028021323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFM6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010953"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011078.1">
        <property type="protein sequence ID" value="ENSXCOP00000010953.1"/>
        <property type="gene ID" value="ENSXCOG00000008270.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114146890"/>
    <dbReference type="GeneTree" id="ENSGT00390000000719"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009346">
        <property type="entry name" value="GRIM-19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12966:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12966">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE 1 ALPHA SUBCOMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06212">
        <property type="entry name" value="GRIM-19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 14" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="144" mass="17185" checksum="0E8FE52D3F248ACB" modified="2018-12-05" version="1">MAGSKVKQDMPPSGGYPAFDYKRNLPKRGLSGYSMFGIGIGIMAFGYWRMFSWNRERRRLLIEELEARIALMPLIQAEHDRRTLRMLRENLEEESIIMKDVPGWKVGERVFHTDRWVPPLSDEMFSLRPREQYLHKRFGFLWYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFR5</accession>
    <name>A0A3B5LFR5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Prostaglandin D2 synthase b, tandem duplicate 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027860687.1">
        <property type="nucleotide sequence ID" value="XM_028004886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFR5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008521"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008627.1">
        <property type="protein sequence ID" value="ENSXCOP00000008521.1"/>
        <property type="gene ID" value="ENSXCOG00000006523.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114136698"/>
    <dbReference type="KEGG" id="xco:114136698"/>
    <dbReference type="GeneTree" id="ENSGT01120000271921"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036094">
        <property type="term" value="F:small molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002345">
        <property type="entry name" value="Lipocalin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022272">
        <property type="entry name" value="Lipocalin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11430">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11430:SF139">
        <property type="entry name" value="LIPOCALIN-15 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00179">
        <property type="entry name" value="LIPOCALIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01254">
        <property type="entry name" value="PGNDSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00213">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017216640" evidence="3">
        <location>
            <begin position="21"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="4">
        <location>
            <begin position="34"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="190" mass="21144" checksum="ECEEE4421378AA3D" modified="2018-12-05" version="1">MTTTGLRMLTALTCVLAACANTTPVPDFSLEKVAGKWYMAGFATNAQWFVNHKDTMKMGIAMMVPTDIDDLDLSYATLNADGTCWRMTHLAKKTDTPGRFTFHSQVWNNDNDMRFVDVVYDSYAVVHTIKTKEGVSEVLNKLYSRSPEISADLQQKFTQFSLDTGILAENIVTFPQNGECPEASVDPNPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFS4</accession>
    <name>A0A3B5LFS4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein fem-1 homolog C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">FEM1-gamma</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of a CRL2 E3 ubiquitin-protein ligase complex, also named ECS (Elongin BC-CUL2/5-SOCS-box protein) complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the fem-1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027889884.1">
        <property type="nucleotide sequence ID" value="XM_028034083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFS4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006719"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006801.1">
        <property type="protein sequence ID" value="ENSXCOP00000006719.1"/>
        <property type="gene ID" value="ENSXCOG00000005196.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114154730"/>
    <dbReference type="KEGG" id="xco:114154730"/>
    <dbReference type="CTD" id="56929"/>
    <dbReference type="GeneTree" id="ENSGT00940000158626"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000104">
        <property type="entry name" value="Fem-1 homolog c (C.elegans)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000076">
        <property type="entry name" value="Fem-1 homolog c (C.elegans)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000163">
        <property type="entry name" value="Fem-1 homolog c (C.elegans)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000173">
        <property type="entry name" value="Fem-1 homolog c (C.elegans)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173">
        <property type="entry name" value="ANKYRIN REPEAT CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24173:SF14">
        <property type="entry name" value="PROTEIN FEM-1 HOMOLOG C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="82"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="115"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="148"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="181"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="481"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="527"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00063767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="617" mass="68569" checksum="34782C9086BFEE27" modified="2018-12-05" version="1">MDLKTAVYNAARDGKLRLLQKLLENKDGHEVTKLMGEKTNGATPLLMAARYGHLDLVEYLLECCCAPVEVGGSVNFDGETIEGAPPLWAASAAGHLKVVQSLLGHGASVNSTTLTNSTPLRAACFDGHLDIVRYLVEHKADLEVANRHGHTCLMISCYKGHKEIAQYLLEKGADVNRKSVKGNTALHDCAESGSLEIMRMLLQYGATMERDGYGMTPLLSASVTGHTNIVDYLTTHQQTSHTERIDALELLGATFVDKKRDLLGALKYWKRAMDLRYTDSNIVHKPEPKHLIMAYDYAKEVTNGEELDGLISDPDEMRMQALLIRERILGPQHPDTSYYIRYRGAVYADSGNFERCINLWKYALEMQQNNLDPLSPMTTSSLLSFAELFSFMLQDRAKGLLGTSVSFEDLMGILTKSVLEIERAVKQIGPMPPDPAQLSKALSIILHLICLLEKVPCTTEQDHFKKKTIYRFLKLQPCGKNGFTPLHLAVDRNTTCVGRYPVCKFPSLTVASILLECGADVNSRDEDDNSPLHIAASNGHPDIMNLLISCGNHFDSTNAFQQTACDLLDEKELARNVIQPINHTTLQCLAARAIIKHSLHYRGNIPEKLEAFVLLHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LG81</accession>
    <name>A0A3B5LG81_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Wiz C-terminal zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LG81"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009605"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009719.1">
        <property type="protein sequence ID" value="ENSXCOP00000009605.1"/>
        <property type="gene ID" value="ENSXCOG00000007319.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01060000248660"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051643">
        <property type="entry name" value="Transcr_Reg_ZincFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055125">
        <property type="entry name" value="Wiz_C_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24396">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24396:SF25">
        <property type="entry name" value="ZINC FINGER PROTEIN 644"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23015">
        <property type="entry name" value="zf-WIZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Wiz C-terminal zinc finger" evidence="7">
        <location>
            <begin position="31"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="78"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="102" mass="11230" checksum="A9378A781DE27A55" modified="2018-12-05" version="1">MLFSKNCKAVRTRSRPGLKKKTLPLAQSPEEMYRLTCRFCDLVFQGPLSVQEDWIKHLQRHIMNTGVPRTGLGMAEVTSLSTNPPTLKTGEDGSSPVANAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LH15</accession>
    <name>A0A3B5LH15_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Thy-1 cell surface antigen</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LH15"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008966"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009072.1">
        <property type="protein sequence ID" value="ENSXCOP00000008966.1"/>
        <property type="gene ID" value="ENSXCOG00000006838.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000171965"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043209">
        <property type="term" value="C:myelin sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051894">
        <property type="term" value="P:positive regulation of focal adhesion assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033292">
        <property type="entry name" value="THY1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19226">
        <property type="entry name" value="THY-1 MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19226:SF2">
        <property type="entry name" value="THY-1 MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017341801" description="Thy-1 cell surface antigen" evidence="9">
        <location>
            <begin position="16"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="17030" checksum="0CA33EC9BFE6DA80" modified="2018-12-05" version="1">MFLLITVLLFGFASAQKVVQLSYCSLAENNLRMDCKYALPAESPEPFCKYTQGERLLDTTDPDEEQHAPYKNRAKARIFPGNVCRLLFKNLPKGKSNFTCNIRLGDSAAATKTSVVEKSKLPARHGHCSLQLCTSCYFTCKKTREAVILLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LH17</accession>
    <name>A0A3B5LH17_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Ras homolog family member V</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027896597.1">
        <property type="nucleotide sequence ID" value="XM_028040796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LH17"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010152"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010271.1">
        <property type="protein sequence ID" value="ENSXCOP00000010152.1"/>
        <property type="gene ID" value="ENSXCOG00000007704.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114158893"/>
    <dbReference type="KEGG" id="xco:114158893"/>
    <dbReference type="CTD" id="171177"/>
    <dbReference type="GeneTree" id="ENSGT00940000157624"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055113">
        <property type="term" value="P:epiboly involved in gastrulation with mouth forming second"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071896">
        <property type="term" value="P:protein localization to adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000561">
        <property type="entry name" value="rho-related GTP-binding protein RhoV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="236" mass="26602" checksum="D270574C24260ECE" modified="2018-12-05" version="1">MPPQMDYFYHESRVPSACGLTREDELDPDVISCMLVGDGAVGKTSMIISYISNGYPAEYQQTGFDVFSGQVQVEGSPVKIQLLDTAGQEEFDEFRALSYAHADVFLLCFSMVDPDSFHNITKKWVPDIRAHNASSPIILVGTQLDRLLDVNVLINLDKCKVKPVLSSRARSLADKIRATDYIECSSLTQKNLKEAFDAAIFAAIKSKTRKGKKRRFSDRRTKAFSRCSWKKFFCFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LH46</accession>
    <name>A0A3B5LH46_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="23">Complement C8 alpha chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">C8A</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Target cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complement C6/C7/C8/C9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LH46"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010182"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010301.1">
        <property type="protein sequence ID" value="ENSXCOP00000010182.1"/>
        <property type="gene ID" value="ENSXCOG00000007721.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160126"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005579">
        <property type="term" value="C:membrane attack complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044218">
        <property type="term" value="C:other organism cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006958">
        <property type="term" value="P:complement activation, classical pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048831">
        <property type="entry name" value="C8A_B_C6_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001862">
        <property type="entry name" value="MAC_perforin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020863">
        <property type="entry name" value="MACPF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742">
        <property type="entry name" value="COMPLEMENT COMPONENT C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742:SF1">
        <property type="entry name" value="COMPLEMENT COMPONENT C8 ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21195">
        <property type="entry name" value="EGF_C8A_B_C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00764">
        <property type="entry name" value="COMPLEMENTC9"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01705">
        <property type="entry name" value="TSP1REPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00279">
        <property type="entry name" value="MACPF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-0180">Complement pathway</keyword>
    <keyword evidence="12" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="17 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0391">Immunity</keyword>
    <keyword evidence="8" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0473">Membrane attack complex</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-1052">Target cell membrane</keyword>
    <keyword evidence="19" id="KW-1053">Target membrane</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="MACPF" evidence="22">
        <location>
            <begin position="63"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="497" mass="55484" checksum="45D8E6893860143A" modified="2018-12-05" version="1">VGTLWDRLACPTATTQCLVKDYCGESFTCKETGRCISQSLRCNGEPDCDDFSDEDECDRINQREDKCSTLIPIPGAERSTQGYNLLTGDFMDQVLDPKYFGGKCEYVYNGEWSKLTYDAFCESLQYNEDEKNYRKPYNYHTYRFVAEATSKGSDEYFEDMQRLLEARQSMSSSNFGISVGISFVNVGVKGNAESEFLNNLTKYQSQDFSFIRVWSKVQTAHFKMRSNQLMLHEDFYIALMELPEQYDFGVYSRFLNTFGTHYVTEGAMGGSLEYVAVLNKTAMAKSNIDAKRFQSCFGASLGISYSLSETSDLGVEVEGTNSSESAVVEDIIKLVKGGITETGSGLLVIRDQATYRKWGASLKYSPALTDFEILPIFEAVRLSTAADHAGARIENLERAIEEYLREFDTCRCAPCRHNGIHVLSGTSCSCICRSGFRGVACEETLRRDAKTDGSWSCWGSWSTCSSGTKSRRRVCNNPSPEGGGAPCLGSANQNRRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKV3</accession>
    <name>A0A3B5LKV3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKV3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011300"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011431.1">
        <property type="protein sequence ID" value="ENSXCOP00000011300.1"/>
        <property type="gene ID" value="ENSXCOG00000008536.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000096"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043291">
        <property type="term" value="C:RAVE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052208">
        <property type="entry name" value="DmX-like/RAVE_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13950:SF9">
        <property type="entry name" value="RABCONNECTIN-3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13950">
        <property type="entry name" value="RABCONNECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="100" mass="10412" checksum="2977739E3C857A31" modified="2018-12-05" version="1">MNLHQVLTGAVNPGDNCFSVGSVNNVPFTAYASGCDVVVLGSNFERLQIIPGAKHGNIQVGCVNCSQRGGQIAASYGSIVCVFEPVHLPDQKYASSVSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLZ4</accession>
    <name>A0A3B5LLZ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Peptidase metallopeptidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="16">Can bind about 5 Ca(2+) ions per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="16">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M10A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLZ4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010546"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010670.1">
        <property type="protein sequence ID" value="ENSXCOP00000010546.1"/>
        <property type="gene ID" value="ENSXCOG00000007976.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154907"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030574">
        <property type="term" value="P:collagen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04278">
        <property type="entry name" value="ZnMc_MMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000007">
        <property type="entry name" value="Collagenase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033739">
        <property type="entry name" value="M10A_MMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001818">
        <property type="entry name" value="Pept_M10_metallopeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021190">
        <property type="entry name" value="Pept_M10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021158">
        <property type="entry name" value="Pept_M10A_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201:SF151">
        <property type="entry name" value="INTERSTITIAL COLLAGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10201">
        <property type="entry name" value="MATRIX METALLOPROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00413">
        <property type="entry name" value="Peptidase_M10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00138">
        <property type="entry name" value="MATRIXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="PGBD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00546">
        <property type="entry name" value="CYSTEINE_SWITCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0177">Collagen degradation</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 18" id="KW-0732">Signal</keyword>
    <keyword evidence="9 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017303977" description="Peptidase metallopeptidase domain-containing protein" evidence="18">
        <location>
            <begin position="19"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase metallopeptidase" evidence="19">
        <location>
            <begin position="104"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cysteine switch" evidence="17">
        <location>
            <begin position="87"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <feature type="binding site" description="in inhibited form" evidence="16">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
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        <location>
            <position position="175"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
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        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
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        <location>
            <position position="193"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>2</label>
        </ligand>
    </feature>
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        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>3</label>
        </ligand>
    </feature>
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        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
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            <label>3</label>
        </ligand>
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        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
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        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
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        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR621190-2"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR621190-5"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="263" mass="29358" checksum="4A8EDB4536CF6EF3" modified="2018-12-05" version="1">MKTFTLSFLLSLLAAVFCMPVSQPTQQDETFAENYLKRFFNLTEERGPTFRSGISSVSKKLMEMQKFFGLQITGNLDADTLAMMKKPRCGVPDVDVSRFSTFGNNLKWDKNDLTYRIENYTPDMSAAEVDESIEKALQVWAKVTPLRFTRIYSGIADIMISFGRGEHGDFYPFDGPDGTLAHAFAPSSGIGGDAHFDEDETFTFRTNKGYVLFLVAAHEFGHSLGLSHSNDPGALMAPVYSYKNPDTFVLPQDDVRGIQSLYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMB2</accession>
    <name>A0A3B5LMB2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Baculoviral IAP repeat containing 5b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the IAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027896586.1">
        <property type="nucleotide sequence ID" value="XM_028040785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMB2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008994"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009103.1">
        <property type="protein sequence ID" value="ENSXCOP00000008994.1"/>
        <property type="gene ID" value="ENSXCOG00000006860.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114158857"/>
    <dbReference type="KEGG" id="xco:114158857"/>
    <dbReference type="CTD" id="246726"/>
    <dbReference type="GeneTree" id="ENSGT00940000161744"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000009">
        <property type="entry name" value="Baculoviral IAP repeat-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051190">
        <property type="entry name" value="Baculoviral_IAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771:SF2">
        <property type="entry name" value="BACULOVIRAL IAP REPEAT-CONTAINING PROTEIN 5.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771">
        <property type="entry name" value="DETERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="17" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="17632" checksum="31BA949B10761D7A" modified="2018-12-05" version="1">MESVDMLTIRYTTFDKMYSYDLREQSFRDWPFREECNCTPEKMAKAGFVHCPSDNEPDVACCFFCLIELEGWEPDDDPWFEHSKRSPTCGFLTMKKADFTELTVSEYCQLEGERLKIYIRKISHKMMAYLRDDMDKVLDRLKSQLETI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNM2</accession>
    <name>A0A3B5LNM2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptophysin/synaptobrevin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNM2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011046"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011173.1">
        <property type="protein sequence ID" value="ENSXCOP00000011046.1"/>
        <property type="gene ID" value="ENSXCOG00000008345.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234637"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001285">
        <property type="entry name" value="Synaptophysin/porin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10306">
        <property type="entry name" value="SYNAPTOPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10306:SF16">
        <property type="entry name" value="SYNAPTOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00220">
        <property type="entry name" value="SYNAPTOPHYSN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017392389" description="MARVEL domain-containing protein" evidence="9">
        <location>
            <begin position="20"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="10">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="204" mass="22871" checksum="6D40ABEBFC120300" modified="2018-12-05" version="1">MSLCCVCFALFAIFAFATCGGYSGQLRVSVDCMEKASSDLNIGIDFGYPFRLHQVSFEAPMCEAMRTERVFLTGDYSSSAEFFVTIAVFSFLYSLVATVVYIFFLNKYRENSQRPLIDFVVTVVFSFMWLVSSSAWAKALSDVKVATDPDEVQLLIPACKDPTNKCGSLHEPRWSGLNTSVVSLKTMSAIMLGNKKRNKIFFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNZ5</accession>
    <name>A0A3B5LNZ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pancreas/duodenum homeobox protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027861119.1">
        <property type="nucleotide sequence ID" value="XM_028005318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNZ5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011166"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011295.1">
        <property type="protein sequence ID" value="ENSXCOP00000011166.1"/>
        <property type="gene ID" value="ENSXCOG00000008442.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114136909"/>
    <dbReference type="KEGG" id="xco:114136909"/>
    <dbReference type="CTD" id="3651"/>
    <dbReference type="GeneTree" id="ENSGT00940000163955"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003309">
        <property type="term" value="P:type B pancreatic cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000176">
        <property type="entry name" value="pancreas/duodenum homeobox protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017995">
        <property type="entry name" value="Homeobox_antennapedia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664:SF12">
        <property type="entry name" value="PANCREAS_DUODENUM HOMEOBOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45664">
        <property type="entry name" value="PROTEIN ZERKNUELLT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00025">
        <property type="entry name" value="ANTENNAPEDIA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="135"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="137"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="194"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="225"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="255" mass="28815" checksum="38B7830ED91F43B3" modified="2018-12-05" version="1">MNREDPFYPSQVFKDSCAYQRSPGDDYSPPPCLYMSRQVHSVFTPPSLGTLEQASLPDVAPAAFTMPGMREDPGVPPLHHPQGLQQQPTLQPPGYIDAGEQNRYHLPFPWMKTTKSHSHTWKGQWTGSYVMTEAEENKRTRTAYTRAQLLELEKEFLFNRYISRPRRVELALTLSLTERHIKIWFQNRRMKWKKEEDRRRARGSEPDQDSSITSGDQGETAGAGVSSSNGPHTSTPPVSPLHGHTLSASGSREPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQ84</accession>
    <name>A0A3B5LQ84_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tripeptidyl-peptidase 1</fullName>
            <ecNumber evidence="4">3.4.14.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Tripeptidyl aminopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Tripeptidyl-peptidase I</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal tripeptide from a polypeptide, but also has endopeptidase activity.</text>
            <dbReference type="EC" id="3.4.14.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.9" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQ84"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009939"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010057.1">
        <property type="protein sequence ID" value="ENSXCOP00000009939.1"/>
        <property type="gene ID" value="ENSXCOG00000007539.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008684"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008240">
        <property type="term" value="F:tripeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04056">
        <property type="entry name" value="Peptidases_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11377">
        <property type="entry name" value="Pro-peptidase_S53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000012">
        <property type="entry name" value="Tripeptidyl-peptidase 1 preproprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015366">
        <property type="entry name" value="S53_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030400">
        <property type="entry name" value="Sedolisin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050819">
        <property type="entry name" value="Tripeptidyl-peptidase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218">
        <property type="entry name" value="PROTEASE S8 TRIPEPTIDYL PEPTIDASE I CLN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14218:SF15">
        <property type="entry name" value="TRIPEPTIDYL-PEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09286">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00944">
        <property type="entry name" value="Pro-kuma_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51695">
        <property type="entry name" value="SEDOLISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0458">Lysosome</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 20" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 20" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S53" evidence="21">
        <location>
            <begin position="98"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="20">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="20">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="20">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00045460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="462" mass="50453" checksum="3F350F781B5598C8" modified="2018-12-05" version="1">VVHRWLQTHGISNCRTVRTQDFLQCSMTAEVAEALLPGSRFHRYVRDGRSIVRSSSPYSVHDEVHEHLDFVGGLHRVPPKGKDANRKRPRSKAGVHLGVTPAVLRTRYNLTATDVGSAENNSQAVAQFLEQYYHPADLAEFMLMFGGTFQHLSEVDRVVGTQGGGKAGLEASLDVEYIMSTGANISTWVFTNPGRHETQEPFLQWMVLLSNMSDLPWVHTISYGDDEDSLSAAYMTRINTEFMKAGVRGLSLLFASGDSGAGCRHLGKENAFRPSFPASSPYVTTVGGTSFKNPFKVTYEVTDYISGGGFSNVFKMPDYQVSAVEGYLKSVTATLPPQSYFNTSGRAYPDMAALSDNYWVVSNRVPIPWVSGTSASTPVVGGMLSLINGQRLSKGLPALGFLNPRLYKLKGRGLFDVTEGCHLGCLDEKVQGQGFCAAPSWDPVTGWGTPNYPALLDALLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQB9</accession>
    <name>A0A3B5LQB9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phospholipase A2</fullName>
            <ecNumber evidence="9">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">The N-terminal C2 domain associates with lipid membranes upon calcium binding.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQB9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011611"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011743.1">
        <property type="protein sequence ID" value="ENSXCOP00000011611.1"/>
        <property type="gene ID" value="ENSXCOG00000008763.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234606"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04036">
        <property type="entry name" value="C2_cPLA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07200">
        <property type="entry name" value="cPLA2_Grp-IVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000030">
        <property type="entry name" value="Phospholipase A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041847">
        <property type="entry name" value="C2_cPLA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF13">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8 9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7 8 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="11">
        <location>
            <begin position="3"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="12">
        <location>
            <begin position="132"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="418"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="750" mass="85021" checksum="7CD1962D1879BA73" modified="2018-12-05" version="1">MDHCDPIYHHIKEKVEHQISHKFTVTVVRAESVTKGALGDLLDTPDPYVELFIPTAPESRKRTKHIDNDINPKWNETFHFILDPCQPNVLELTLMDANYVMDETLGTASFDITKLKVGQKKLETFKFGKVRLSLSTNLDLRFSLALCDKEKEFRHTRRERVMLGIKKLLDMEKPCFLPSSPQEVPVIAIAGSGGGFRAMVGFSGVMKALFESGVLDCTTYVAGLSGSTWYMSALYSHPDFPDKGPKDINSELMKRVSGNPLRLLLPQHITNYIQALWTKKASGQPVTFTDVFGMLIGETLIPARMDIKLSKIQGKINQSQSPLPLFTCLHVKPDVSELMFADWVEFTPYEIGMAKYGTFMTPDLFGSKFFMGTVVKKYEENPLHFLMGVWGSAFSILFNRVLGVKDTAGGSTMEEELEQIKPQHIVGEDSLDNDDEPRKGGTENQDSEEEYSRTAQASWVQRMFTSLFSDSALFNTREGRAGKVHNFMLGLNLNTSIPFSPTTEMSHPLLTPEDEVDAVTDPDEFDRIYEPLDVKSKKIHVVDSGLTYNLPYPLLLRPQRGVDLIISFDFSARPSDSSPPFKELLLAEKWARMNKLPFPKIDPKVFDREGLKECYVFKPKKGEKNCPTVIHFVLVNINFRKFKAPGVPRVTEKEKELADFDIFDDPESPFSTFNFQYSNEAFTRLHDLMEFNTLNNLEVIKEAIKDCIVAQKEIPSSHSLSFSLSNIPKKKFFKRDPDSNPWNHLGKDGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQR6</accession>
    <name>A0A3B5LQR6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Fibulin 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fibulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQR6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013135"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013296.1">
        <property type="protein sequence ID" value="ENSXCOP00000013135.1"/>
        <property type="gene ID" value="ENSXCOG00000009658.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156047"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000341">
        <property type="entry name" value="Fibulin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000078">
        <property type="entry name" value="Fibulin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000139">
        <property type="entry name" value="Fibulin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000010">
        <property type="entry name" value="Pro-epidermal growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000020">
        <property type="entry name" value="Anaphylatoxin/fibulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026823">
        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050751">
        <property type="entry name" value="ECM_structural_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056612">
        <property type="entry name" value="FIBL-2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055088">
        <property type="entry name" value="Fibulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24034">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24034:SF158">
        <property type="entry name" value="FIBULIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12662">
        <property type="entry name" value="cEGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24532">
        <property type="entry name" value="FIBL-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22914">
        <property type="entry name" value="Fibulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00104">
        <property type="entry name" value="ANATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01178">
        <property type="entry name" value="ANAPHYLATOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Anaphylatoxin-like" evidence="11">
        <location>
            <begin position="317"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="563"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="648"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS01178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="890" mass="98171" checksum="94DF8685EAF59B8C" modified="2018-12-05" version="1">MNTILIPNNCGRDYFVKNEYLCYIALERPGLTLLRCALLLLFPPVSLSQRDCSSVDCPPLENCIKEAPESDGCCASCLQKGCRCEGYQRYDCTRAGFKNGIVPEGQSYFVDYGSTECSCPVGGGPISCRYIGCPDLPPNCIETLEPEDGCQQCERIGCVHRGEKYEAGHSFHVEACRICHCPDEGGKLMCYPDPDCDPQLTLITDDNAPNGRDGFFTRFDQLSASYNRPSSGNLPHFIPLFFFPFISLRVNVTIFLLSACFNYAWSLCLSVVEQQCCLSHVKESRCDSGVAAARAGRACEADGNDSCADEYQVCCSCCAIGLLIRSEGQGCNAHKYLSYPCGHIFLSCCEDEEGQSQSPLWTQEKSRPTALPRPGSSYNFQPLSSSALHGCSQRCAAAAGQARCSCFPGFSLMTDRRTCKGKGSNFLPEGEGEMVESGMFSLFDHVVCRCGRSVIQCTCGKMQSWRSLFNVDECQGNTHSCRPNQRCVNTPGSFLCELQVRCPVGYQMKNGVCQDINECSSLSEPCTAGSACINTVGSYTCRQKILMCNEGYHSSADGTKCVDTDECQMGTHRCGAGQICRNLPGSYRCDCQTGYQYDAFRKVCTDINECWRFPGRLCAQTCENTKGSYRCSCTTGFSLALDGNNCEDVNECDGSPCSQECVNIYGSYQCYCREGYYLKEDRHTCEDIDECSQTIGNLCVFQCVNVVGSYRCSCPPHGYVTAANGRSCKDIDECMTGSHNCSFSQTCYNLQGSFKCLSFDCPQNYKKVSDTHCERMGCPPNSIDCQNSPLRITYHQLNLKTNTITPGQIFRIGPSPVYSGDNIVISIVKGNEGGYFSARKLNSFTGAVYLQRKVGDPKDFLIDVEMKLLRKGTFTSFLSRLYIFITSNKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRY1</accession>
    <name>A0A3B5LRY1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">WD repeat domain 43</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UTP5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRY1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014833"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015019.1">
        <property type="protein sequence ID" value="ENSXCOP00000014833.1"/>
        <property type="gene ID" value="ENSXCOG00000011240.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004254"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052414">
        <property type="entry name" value="U3_snoRNA-assoc_WDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44267">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44267:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="115"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="6">
        <location>
            <begin position="460"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="588"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="404"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="588"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="604"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="614"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="626" mass="69087" checksum="9751FD5BE89E81AD" modified="2018-12-05" version="1">MATDGVSLSLKLPCVFSPKSRQYLAVCAQDGRLRVWNTDSKTLHQEYVPSAHLSATCTCVAWGPCRTVKEGPQRKKRKSEAAQVEEKADLLAMGTAAGTVLIYSTAKGSLHCTLDGGHSGGVNCVHWHPEDSLLYSGSDDTNIVEWDLQTGKTRSKWKADRAAVTSLCVSPDGKLLLSAGHVIKMWDLETKEVYRKFTGHSTAVTTLRFATTRPPDSNGLYFLSGAAHDRLLSVWQVREDGKDKNSVVSFTLTDEPQHVDLITSSSKEEAVRLAVVCKDGQLHLFEHFLNGPCKKPLSPSCSVQMSDTKESPMPIPLLAAALGADPRSVLLAYGNYLQPVMENAEINTAERHVCLARDLKTNLSLSMETTVSKVKTPIVNAKSKVLIPGLPGHKAPVVSAPQGTEKRKKDAGNKEMSIAERLGEIDLEASSSQKGAPKATASLQTDNYAVLLMQGLESKDANILNKVFQTRKEVEIKKTVARLPLPAVIPLVEELTTRLQGNPLVACMMVRWLKAVLVHHTSYLASLPDLVTQLGVLYHMIESRVKMFHKLTKLHGKLHLLTTQVRGLKSSLKLFFFFFFLSQHWEEEEAMQEDEGDEEDEDPDSRTEPKANGDEDMEHENESEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSC2</accession>
    <name>A0A3B5LSC2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Actin binding LIM protein family, member 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSC2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013720"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013888.1">
        <property type="protein sequence ID" value="ENSXCOP00000013720.1"/>
        <property type="gene ID" value="ENSXCOG00000010380.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182850"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030032">
        <property type="term" value="P:lamellipodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09327">
        <property type="entry name" value="LIM1_abLIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09328">
        <property type="entry name" value="LIM2_abLIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09329">
        <property type="entry name" value="LIM3_abLIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09330">
        <property type="entry name" value="LIM4_abLIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032402">
        <property type="entry name" value="AbLIM_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051618">
        <property type="entry name" value="Actin-binding_LIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24213">
        <property type="entry name" value="ACTIN-BINDING LIM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24213:SF0">
        <property type="entry name" value="ACTIN-BINDING LIM PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16182">
        <property type="entry name" value="AbLIM_anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02209">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00153">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0440">LIM domain</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="13"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="73"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="142"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="HP" evidence="11">
        <location>
            <begin position="487"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="331"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="360"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="555" mass="62895" checksum="308F5527BF210BAD" modified="2025-10-08" version="2">MNTPLGERSSEPIRCQRCREVCKGEVVRVQDTHFHVKCFTCTVCNCDLARSGFFQKKGEYICTADYQRLYGTRCDRCGNFITGEVVSALGRTYHPKCFVCSMCSKPFPIGDRVTFSGKDCVCQQCSHTLVKSNEPIKIHGPSHCAGCGAEIKQGQSLLALEKQWHVSCFRCRTCNMVLTGEYISKDGAPYCEADYHAQYGVKCETCYRYISGRVLEAGGKHYHPTCARCARCNTMFKEGEEMYLAGCEVWHPLCKQAARAERRLRHRRLSETSISPPGSSIGSPSRVICDYDHPDAKQLRCPIPGYIDSPTYSRQGMSPIMPRSPQHFGYPATGDPNIYRKPPIYKRTATKSRTSEDILRSSSLSTYSPEPYTQSESDYYPYTSSPRAYRVPRRRFSTGGDEESWSQGLQRIGSGIGRMILKEEMKARSGSYDNDPWGSARNSRCGSKETLNTTGYGTTAYNNTVNDFGCKSASLPGYRRNGIQRNFPSRQVYPYESLIVTTRGRNKLPKDVDRARLERHLSPEEFIQVFGMTVEDFDRLALWKRNELKKQARLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTC5</accession>
    <name>A0A3B5LTC5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GP-PDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTC5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014080"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014255.1">
        <property type="protein sequence ID" value="ENSXCOP00000014080.1"/>
        <property type="gene ID" value="ENSXCOG00000010663.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156251"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008889">
        <property type="term" value="F:glycerophosphodiester phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08574">
        <property type="entry name" value="GDPD_GDE_2_3_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23344:SF13">
        <property type="entry name" value="GLYCEROPHOSPHODIESTER PHOSPHODIESTERASE DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23344">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="10">
        <location>
            <begin position="56"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="405" mass="45051" checksum="D338DDAAFA6B70F2" modified="2018-12-05" version="1">MAVLSWPTALHFFRMNKKRQVVVLALYLSVLFSLYLVPLGMYSPCIKEAGTLGPAPTLIGHRGAPMLAPENTLMSFEKAVESGGGGLETDVTIYDGVPFLMHDSTLRRTTNVAEVFPNRTHLDAAMFTWAEIQQLNAGSWFLLRDPFGTASSLSETDRSQAQNQSVPSLAQFLEVAAASGKLVLFDLRRPPYGHPYRRSYINATLQVIQAHINSSQVLWLPSEDRALVSGLDPALQQTSGLKASIQELKDDGISWLNLHYSTMSQQQIKLQSVNISTNLYVISQPWLYALAWCAGAQSITTNSVHILSSINQPLFLMQTPEEYTLMWILTDFVSAFLIFAIFTFHWRERGLPFVSGSRHTNDNGPYSKFRTLSDVWSISSVNVRPDLRSSPSSPSGPHLPTITEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTT7</accession>
    <name>A0A3B5LTT7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Leptin receptor</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTT7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011234"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011365.1">
        <property type="protein sequence ID" value="ENSXCOP00000011234.1"/>
        <property type="gene ID" value="ENSXCOG00000008298.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111209"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043010">
        <property type="term" value="P:camera-type eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048839">
        <property type="term" value="P:inner ear development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033210">
        <property type="term" value="P:leptin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001556">
        <property type="term" value="P:oocyte maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030728">
        <property type="term" value="P:ovulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060828">
        <property type="term" value="P:regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009749">
        <property type="term" value="P:response to glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003323">
        <property type="term" value="P:type B pancreatic cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041182">
        <property type="entry name" value="LEP-R_IGD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF35">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18589">
        <property type="entry name" value="ObR_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017349588" evidence="11">
        <location>
            <begin position="27"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="801"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="210"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="501"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="972"/>
            <end position="1053"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="972"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="992"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1020"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1044"/>
            <end position="1053"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1140" mass="128946" checksum="F5D087EF0A0BF9B2" modified="2018-12-05" version="1">VCLPRTWSVMLTVLVHVLLMLLAVQCLRPGAPVHSGALDVPWQDEVCCDSPTANAYIEEGTSQSDLNLPLSPRCKFRSSSAESKPLVSLGGKSLCSKYCTCLDILCRFDEPWSNLTCDLKFSGLSSGIMKPSCLLFTLVSDGEDVSSLENDTDYHASEHKAVCDAEDSLMCSIHLNSTRIFAVRLTVNVSNVLASPVLLRISPRSVKPSPPFNLSHIQTVEGDLILRWKEPLDFSDNLLAYEIRYSHENTNKNTHKNWQVVSALEQPNVSLELKPLLKYTIQVRCTTQTQPVLWSDWSDPYHIYLNNVSYIPQQVVARAGDNVTVYCVLNGQSSNANTAMWSLNRMVPLDPSLYHPVNQRVSQITFTASETGLYDLLTCTQEWNIPYSKIYVEGGFINITCVTNGDIDTMTCRWENKEWEIVKLRSKSAEMSCDEMEERVRGGEDVGEMGHECMPVKSTEKVCIFHPLKMNCYKLWLELHQGPIRCKPVYVSPINHVKPYPPTDVMAVSRSSGILRVTWKPPSLPVEGLQCQFRYYLLGVLEKWKFEKPVWVSWAEVEVPDMCQIYVVQVRCMHTSGIGYWSEWSESVHSTAQNSRAPERGPDFWRIRHDDPYRNQSNITLLFEYFSANWNTYCVDGFIVQHQALNGTVLRRQIDMVSSYSFEWNQELHIVTVEAYNILGSSVNNVNMTLEKQPKRRCVHSFHVLVINSTCVSLSWSLLHISAVPLFMVVQWFPRKQQDFQDFGLSGKTWAKLSYTEPPTQLRGDFFSSKEYTFHLYPVFADREGEPVVTIGIFFTGNPPAYMMLMFISLICIAMFVTLVLSQNQMKRFVWKDVPNPNKCSWAKGLDFKTMDGFEYLFRPPEGLQASPLPPEKISKVTVVNKTLTKAFVQTPTLLSPPSVVASTNSFLPCLDPRTGQLLECETALGGDTSPDPLTTSHLTINKLQPANVLVEQSSGITNSSAQSSVTYSKVLHTDTNPDHPPLHLHYKEGSRSSYSDEGNFSANNSDTSETFPGGVWEVDSYRGTESDDPRRSYSYNSEKELSDASEQEDDVDVGQKKALHYLNIGHPPEDEENEEEESQTELLKSAHLIREDCSVELYPLLNPDELMDPKTLVQVSTCSFASLYMPQYRKPDVNHIPHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUT8</accession>
    <name>A0A3B5LUT8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box only protein 9</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2 7">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUT8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015753"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015947.1">
        <property type="protein sequence ID" value="ENSXCOP00000015753.1"/>
        <property type="gene ID" value="ENSXCOG00000011902.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014256"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22089">
        <property type="entry name" value="F-box_FBXO9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000012">
        <property type="entry name" value="F-box only protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045464">
        <property type="entry name" value="Hrt3/FBXO9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12874">
        <property type="entry name" value="F-BOX ONLY PROTEIN 48-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12874:SF29">
        <property type="entry name" value="F-BOX ONLY PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19270">
        <property type="entry name" value="FBO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="5 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="8">
        <location>
            <begin position="168"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="356" mass="41284" checksum="837AA9089F5A1F98" modified="2018-12-05" version="1">MKNYAGFQQRCLPLQADENADIGGMLEDEDDSSDDPNLQLELNVFRAQWMSELKPSPGTSDRLQRARGVKRTQDLAREEKATELFLRAVQEEQNGAVYEAIKFYRMAMQLDPDIEFKINYSRPPDADRGGGNYDTDGEIEDLLTYFEQQLTLESSFPKICTPELEATQTHISALPREILMYIFRWVVSSELDMRALEQLSLVCRGFYISARDPEIWRSACLRVWGRNCTKVVPFKSWREMFLQRPRVRFDGVYISKTSYIRQGEESLDGFYRAWHHVEYYRYLRFFPDGFVLMLTTPEDPLSVVPRLRTRNISANSQLHVDHVYCSEKCGLCLMLIFTFPIYGGGNGGLRVWAGSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVZ7</accession>
    <name>A0A3B5LVZ7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">14 kDa phosphohistidine phosphatase</fullName>
            <ecNumber evidence="5">3.9.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Phosphohistidine phosphatase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Protein histidine phosphatase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Exhibits phosphohistidine phosphatase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(pros)-phospho-L-histidyl-[protein] + H2O = L-histidyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47964"/>
            <dbReference type="Rhea" id="RHEA-COMP:9745"/>
            <dbReference type="Rhea" id="RHEA-COMP:9746"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64837"/>
            <dbReference type="EC" id="3.9.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(tele)-phospho-L-histidyl-[protein] + H2O = L-histidyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47960"/>
            <dbReference type="Rhea" id="RHEA-COMP:9745"/>
            <dbReference type="Rhea" id="RHEA-COMP:10719"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83586"/>
            <dbReference type="EC" id="3.9.1.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the janus family.</text>
    </comment>
    <dbReference type="EC" id="3.9.1.3" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVZ7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012009"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012147.1">
        <property type="protein sequence ID" value="ENSXCOP00000012009.1"/>
        <property type="gene ID" value="ENSXCOG00000009054.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002738"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101006">
        <property type="term" value="F:protein histidine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.20.20:FF:000001">
        <property type="entry name" value="14 kDa phosphohistidine phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.20.20">
        <property type="entry name" value="Janus/Ocnus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007702">
        <property type="entry name" value="Janus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038596">
        <property type="entry name" value="Janus_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12258:SF10">
        <property type="entry name" value="14 KDA PHOSPHOHISTIDINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12258">
        <property type="entry name" value="JANUS-A/JANUS-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05005">
        <property type="entry name" value="Ocnus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143724">
        <property type="entry name" value="PHP14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR607702-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR607702-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="129" mass="14487" checksum="BF17B2A57E881529" modified="2018-12-05" version="1">MCSQTKAAAVMANIPRADIDPSGVFKYIVIRVYSREEGDDSEIDIVRGYGWAEYHAGDIYDRVSAELEDGGLLDCECIGGGRIKHNPEAKKIHVYGYSLGYGRANHAETTEKLKEKYPDYEVTWDNEGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWQ0</accession>
    <name>A0A3B5LWQ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Disabled homolog 2-interacting protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWQ0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015562"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015755.1">
        <property type="protein sequence ID" value="ENSXCOP00000015562.1"/>
        <property type="gene ID" value="ENSXCOG00000011763.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155853"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021887">
        <property type="entry name" value="DAB2P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF26">
        <property type="entry name" value="DISABLED HOMOLOG 2-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12004">
        <property type="entry name" value="DAB2P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Disabled homolog 2-interacting protein C-terminal" evidence="3">
        <location>
            <begin position="70"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="135"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="403"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="276"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="160"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="196"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="431" mass="47227" checksum="15789626F8BFC25B" modified="2018-12-05" version="1">MMSLPAGMRGSPAPSSSYSEPNENEACYDLANGGRREGPELTNESRSLSLVDLQDSSPSDAPDDTQWQRRTGLLPLSFQNPVYHMTTPSPRQPQQTDATPSDGSAGSQGNCEERGAMATKPAFLTQMSVGFRGRLVTFAPPTGSSAPPRQNSGPQRRIDQPPPPSSAPPGPPRGRTPPSMLSGGGSAYPPRPASGSMMSSSPDWPNSGQSRLRQMSSSSKGDSPEKQRPANQNRVRPCVQAPSPCALDRTAAWLLNMNSASSYGEVEDDRHDDATVEKYQQEIALLQEKLRVAALRQEECEARLVVQDQQNQRMLQEYQARLEDTESRLRRLQDDKDLQMNSIISRLMAVEEELKKDHSDMQAVVDQKQKVIEAQEKRIASLDAANTRLMAALTQLKERYGVTSQRNGLSPSNTSSLQITENGEFRNSGNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LX77</accession>
    <name>A0A3B5LX77_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tripartite motif containing 59</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LX77"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016523"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016733.1">
        <property type="protein sequence ID" value="ENSXCOP00000016523.1"/>
        <property type="gene ID" value="ENSXCOG00000012473.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165917"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16763">
        <property type="entry name" value="RING-HC_TRIM59_C-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF229">
        <property type="entry name" value="TRANSCRIPTION INTERMEDIARY FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="10"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="398" mass="43209" checksum="CC55C747FEF1BA47" modified="2018-12-05" version="1">MDSLEEDLTCSVCYSLFSDPRVLPCSHTFCKNCLENLLQVSTNYSIWRPLRLPIKCPHCRSVVELPPSGVDALPSNVSLRAIVEKKHADLRLAVSVPEGQRAAGAVLPGAPQAAAQHVLRPGPAADLRPALGAEVESGGGWEVCELGEQLEQEKARCDTLLRQDRQEVNQFFVSLEEVLARKKQAYLEALSEAAAEVAATYDPLIHTVKELQEEQLDLVSLGSSVEEEDSPLVFLEKVHQFRDRVEKFIRTPLPSGINLSVTPRAAAYLQQNWPAVTISSLEQAPVPKLCCCTRCDGGVGTEADPPVPPAWDRWRQLKPTASVALLGLLLLLAALWANPVGGASLGFSLLSRLSQLAQSLSGSLVSSVWDTMEAECAEMGAAVERIQAVHQLQLCIPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYJ7</accession>
    <name>A0A3B5LYJ7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">OCEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ELL/occludin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYJ7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012869"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013026.1">
        <property type="protein sequence ID" value="ENSXCOP00000012869.1"/>
        <property type="gene ID" value="ENSXCOG00000009732.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154828"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2670">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042065">
        <property type="entry name" value="E3_ELL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031176">
        <property type="entry name" value="ELL/occludin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019464">
        <property type="entry name" value="ELL_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010844">
        <property type="entry name" value="Occludin_ELL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288">
        <property type="entry name" value="OCCLUDIN AND RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288:SF8">
        <property type="entry name" value="RNA POLYMERASE II ELONGATION FACTOR ELL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10390">
        <property type="entry name" value="ELL"/>
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    </dbReference>
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        <property type="entry name" value="Occludin_ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="occludin/ELL-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51980">
        <property type="entry name" value="OCEL"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="OCEL" evidence="8">
        <location>
            <begin position="484"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="152"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="324"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="327"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="353"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="365"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="382"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="404"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="435"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="447"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="598" mass="67162" checksum="519FF858D4DA1F19" modified="2018-12-05" version="1">MAALSEDGSYGLNCGQQGAERVSVLHVKLTETALRAIENYQSSSNVPSSRPKVQFKGLQGHIKIPRTDSSDAFHNFDFYLSNVGKDNPQGSFECIHQYVSSSGASHLALLATVQDKVTVCATNDSYQVTRERMTQAAEDTRERGTKVIKPGGQYRGKVHTRKPALSAPDVVPERKRSTPINPANTIRKCLSNNPVSQKPFRDRIIHLLALKSYKKLEVLGRLQRDGINQKDRNSLGTTLQQVAILNPKDNTYSLKESIYRDVQRDWPGYSEDEKIQADRILARKLGLPTERVSSNSLPKDGFPSSPQKHQADFDFIDPLAPKKARISHLSSRGPSASSSDRREDESSPRSKHSSLPPSVTSGPPTHLPIPSHPPAPSHRQLSPASNSNSPSTPEGCGTQDLPMDQSSSCRDPSPSPFSLASTVVTSPPLSSSTSKKFKKKSKKHKDKDRERVKGKQTERASSSIPPDAVAEESHRVRKRRSAEEDIEVAYAPLASLDQRQSYKDDFNAEYDEYRQLHARVESITRRFTQLDARCRKLVPGTKEYQKVQEEVLKEYKKMKQHNPNYHEEKQRCEYLHNKLAHIKRLIADFDQRRAQTWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYR3</accession>
    <name>A0A3B5LYR3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYR3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016307"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016513.1">
        <property type="protein sequence ID" value="ENSXCOP00000016307.1"/>
        <property type="gene ID" value="ENSXCOG00000012319.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175215"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="77" mass="8679" checksum="51641D9CF60160F2" modified="2018-12-05" version="1">MKSVGVVDGGLFTESYCNICNAQLISESQRTAHYENCMFLLKNAFLQHDAPTICHCGLFRKMCIIFPHSLLPVMQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZI3</accession>
    <name>A0A3B5LZI3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MyoD family inhibitor domain containing</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MDFI family.</text>
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        <property type="protein sequence ID" value="ENSXCOP00000016577.1"/>
        <property type="gene ID" value="ENSXCOG00000012511.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026134">
        <property type="entry name" value="MDFI/MDFIC"/>
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    <dbReference type="PANTHER" id="PTHR15304">
        <property type="entry name" value="MYOD FAMILY INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15304:SF4">
        <property type="entry name" value="MYOD FAMILY INHIBITOR-LIKE"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF15316">
        <property type="entry name" value="MDFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="165" mass="17185" checksum="EAA1711A01D34140" modified="2018-12-05" version="1">CRCPIYAAPPGSDQTFSSAGEARWPGRGRNGPVVPCRRRPVSRAAPMTACLSRKTFSSELKCFQTLLSFPIVPADCCVHCLLACLFCELLSMCSALGECLACGVGGANCCDCAACCCCCVEAAGEAACTEEACQAVLDCGMLENCCASSDCLEVCLECCAIVFPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M049</accession>
    <name>A0A3B5M049_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Calpain-3/13-like C-terminal EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M049"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017478"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017700.1">
        <property type="protein sequence ID" value="ENSXCOP00000017478.1"/>
        <property type="gene ID" value="ENSXCOG00000013183.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160421"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110158">
        <property type="term" value="C:calpain complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054069">
        <property type="entry name" value="CAPN3/13-like_C_EFh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46735:SF3">
        <property type="entry name" value="CALPAIN SMALL SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46735">
        <property type="entry name" value="CALPAIN, SMALL SUBUNIT 1 A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21875">
        <property type="entry name" value="CAPN13-like_C_EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calpain-3/13-like C-terminal EF-hand" evidence="8">
        <location>
            <begin position="53"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF21875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="121" mass="13599" checksum="2162B39167937204" modified="2018-12-05" version="1">DACRCMVALMDTSVTGRLNSSEFIRLWNKVIIYKDIFFQTDVSRTGTLSLTELRNALVASGMRVSDDMLNLMALRYGASSGHMTLESFISLILRLDCMFSESAGSGPDQMSNRWIYVSMYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M131</accession>
    <name>A0A3B5M131_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydrouridine synthase 4-like (S. cerevisiae)</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M131"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016960"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017179.1">
        <property type="protein sequence ID" value="ENSXCOP00000016960.1"/>
        <property type="gene ID" value="ENSXCOG00000012786.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074907"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF31">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20A_20B) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="1">
        <location>
            <begin position="19"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="115" mass="12883" checksum="ABDFFDCEA865B396" modified="2018-12-05" version="1">MKTGSIMDMFEQGKVLKICAPMVRYSKLAFRSLVRKYNCDICFTPMIVATDFLRSVKARDSEFTTNERDRPLIVQFAAHDAQTLADAACVVAPFSDGVDLNCGCPQRCCLWGHKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M176</accession>
    <name>A0A3B5M176_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M176"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018067.1">
        <property type="protein sequence ID" value="ENSXCOP00000017843.1"/>
        <property type="gene ID" value="ENSXCOG00000013447.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165002"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005332">
        <property type="term" value="F:gamma-aminobutyric acid:sodium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006836">
        <property type="term" value="P:neurotransmitter transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002980">
        <property type="entry name" value="Na/ntran_symport_GABA_GAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037979">
        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="SODIUM/CHLORIDE DEPENDENT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF316">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 6 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01195">
        <property type="entry name" value="GAT1TRNSPORT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00754">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="8" id="KW-0769">Symport</keyword>
    <keyword evidence="3 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="293"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="322"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="381"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="423"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="453"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="499"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="538"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600175-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="592" mass="66820" checksum="DEB5EF385091C188" modified="2018-12-05" version="1">MGKTGQATVDLNSPSEVKQAVPSEDLVSTAPPADQKKEKEVPPDRGSWKGKFDFLLSCVGYAIGLGNVWRFPYLCGKNGGGAFLIPYFMTLVFAGIPLFFLETALGQFTSVGGLGVWKLIPMMKGVGLAAVVLSFWLNIYYIIIIAWALYYLFNSFSSELPWQSCDNPWNTESCFSNYSLTDTTNLTSAVTEFWERNMHQMTDGLETPGELRWPLVGTLALAWVLVYFSIWKGVEWTGKVVYFSATYPYFMLFILFFRGVTLPGAIDGILFYITPDFNKLIRSEVWLDAATQIFFSYGLGLGSLIALGSYNTYNNDVYKDSIIVCCINSCTSMFAGFVIFSIVGFMSYITKKPVQELAASGPGLAFLAYPQAVTQLPMSSLWAILFFSMLMMLGLDSQFCTVEGFITALMDEYPLVLRKRKKLFILIVCFISFVIGFSNITQGGLYVFKMFDYYSASGMCLLFLVFFETISISWFYGAERFYKNIEDMIGYRPCVWWKLCWLFFTPLICLGVFTFSAFEMTPLTLGKYVYPLWGQVIGWFMALSSMILIPGYVIYMFCTTKGSIKQVHLPAALQPPTGSRNLFIKRLIKQKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M1Z1</accession>
    <name>A0A3B5M1Z1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSXCOT00000014219.1">
        <property type="protein sequence ID" value="ENSXCOP00000014044.1"/>
        <property type="gene ID" value="ENSXCOG00000010633.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286959"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000965">
        <property type="entry name" value="Neurotrophin receptor-interacting factor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001480">
        <property type="entry name" value="Si:cabz01071911.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001506">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000309">
        <property type="entry name" value="zinc finger X-chromosomal protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF49">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF49.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="197"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="253"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="71"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="389" mass="45074" checksum="16532E65FC0416F7" modified="2018-12-05" version="1">MLVKEHHLRVKREHERNVPEIKNVSKVSYSSKVTADKSILQQLEYFKREAEKYAFRNKGLIEVMVEEYRVRLKYEEQLKSHTEQASKFKAQEETVKSLQGQVADLKVKLKLPFSCVNCGKSFTLKWELTQHMMIHTGEKPFSCVNCGKSFSQKRELTEHLMIHTGEKPFSCVNCGKSFRRKPHLTQHMMIHTGEKPFSCVNCGKRYSGKRELTRHMMIHTGKKLHCCSECSKTFSCRQYLLKHNRIHTGEKPFSCSVCDKTFTWKPSLTLHMKKHSEEKPFRCSVCDKTFTRKLSLTLHIRMHSEKLFSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGPGFDSRPGSFCMEFACSPCACVGSLRVLRLPPTVQKHDCQVNWFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M296</accession>
    <name>A0A3B5M296_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 large subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M296"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018218"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018449.1">
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        <property type="gene ID" value="ENSXCOG00000013725.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111066"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="BRCT_RFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Replication factor C subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="8">
        <location>
            <begin position="347"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="183"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="251"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006116"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
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    </evidence>
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            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="450" mass="49531" checksum="5B955D881319742E" modified="2018-12-05" version="1">QDIRRFFGPVSGKPAPNVDRKPDEKRKKKTSSEEDVKKKKKKEEEEEEGSKKSSKGKAATNSDSDNFQSLKKASKPKQNGANKKSEEDSCSPKVKERVKSPAKPPLSWGKTEIKSPKVPTTLKLAPPPPPKQTLTSVLDYFGSGEIHRSDKKLVASTKRKAPTQELDDLISDEELARQLQMEEDMEVEKQVHEDEEFARTLAMLDTEPQPKKSRVSVPSKTSRTPPEDVISPSPQKSPAAVRASSKLAMMKKQDEAKDEGAKSKTPTSPRKNKISPKKEPRASSISDMKFTPKAGTTPAALKTSPKKPEVITSPDDAEKKKMHTLAFRNYLNRDGPRALGSKEIPEGAENCLEGCVFVLTGVLESMERDDTKALIERYGGKVTGNVSKKTNYLVQGRDSGASKLDKAESLGTVILDEDGLLELIRTKPGKKSKYEIAAEAEVRTPTLVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M551</accession>
    <name>A0A3B5M551_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TBC1 domain family member 20</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M551"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018500"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018733.1">
        <property type="protein sequence ID" value="ENSXCOP00000018500.1"/>
        <property type="gene ID" value="ENSXCOG00000013923.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014944"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="TBC1 domain family member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.1310:FF:000001">
        <property type="entry name" value="TBC1 domain family, member 20"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045913">
        <property type="entry name" value="TBC20/Gyp8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913:SF10">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 20/GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="9">
        <location>
            <begin position="61"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="321"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="338"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="395" mass="45244" checksum="8FCA99E58CC7B3D6" modified="2018-12-05" version="1">QTVVMFVFSTRKGRWVRGDGQLDGDWETKRKRKIADITQALSVDPVDIATLRRMAISEGGLLTDEIRCQVWPWLLNIPPHLLDQEPDYNQVLLDVQRSLRRFPPGMPDEQREGLQEELIDIILRVLQRNPQLHYYQGYHDIVVTFLLVVGERLATVLVEKLSTHHLRDFMDPTMDNTKHILNYLMPIIERVNPEVQDFMQQAEVGTVFALSWLITWFGHVLSDFRHVVRLYDFFLACHPLMPIYFAAVIVLHREEEVLDCECDMAMVHHLLSQIPQDLPYETLISRAGDLFVQFPPSELAREAAAHESMATSTFKDFELASTQQRPDTVLRRRRRQKQGSPESSTASPVMTVAQPSTARRFVRLAVMGLTVALGAAALAVVNTALEWAPKLDLFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M614</accession>
    <name>A0A3B5M614_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M614"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019878.1">
        <property type="protein sequence ID" value="ENSXCOP00000019633.1"/>
        <property type="gene ID" value="ENSXCOG00000014746.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000170113"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027814">
        <property type="entry name" value="DUF4562"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34833">
        <property type="entry name" value="GENE, 17359-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34833:SF1">
        <property type="entry name" value="GENE, 17359-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15123">
        <property type="entry name" value="DUF4562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="127" mass="14505" checksum="A98DEDCB1EA0489E" modified="2018-12-05" version="1">MHTNACGFNSHRQCSSYGIGDYRPRLNYFPLYIGVGATSPEATSDLSYLCRAAPDTPPPAPRQSFVGEVGWGWQYNQLLNGDRLLSNMQIKETQIEKYIVCKTDVAKQWDMSLKIKKKLWENKIMIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M710</accession>
    <name>A0A3B5M710_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Transmembrane protein 163a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Synaptic vesicle membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMEM163 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M710"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019530.1">
        <property type="protein sequence ID" value="ENSXCOP00000019292.1"/>
        <property type="gene ID" value="ENSXCOG00000014497.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001170"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042552">
        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014003">
        <property type="term" value="P:oligodendrocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026765">
        <property type="entry name" value="Tmem163"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31937">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31937:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="237" mass="26321" checksum="E341BF5ACEE4AA3B" modified="2018-12-05" version="1">MTHFSLSNETQGFALVVLTQNALRYSPLPASGAASGLLKIMTFLNTHEEKQKRPCNILCVCFLFAGLLESSMRLKPQEAQNYRKKALWVSWVSIVVTLILAVAAFSKHLKQMSFKSLVREIFSLLTAISTYSVDDFLYSVSIVSGLSCAVLAVAKFMLGKKLTSRALITDGFNSLVGAIMGFSILISAEVFKQHPDVWFLDGTIGVLIGLIILAYGVKLLKDMVPRVRQTRNYERFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7Z7</accession>
    <name>A0A3B5M7Z7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Cadherin 18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CDH18</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Cadherins are calcium-dependent cell adhesion proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7Z7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020403"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020651.1">
        <property type="protein sequence ID" value="ENSXCOP00000020403.1"/>
        <property type="gene ID" value="ENSXCOG00000015288.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157512"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002009">
        <property type="term" value="P:morphogenesis of an epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000001">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000008">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000009">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000014">
        <property type="entry name" value="Cadherin 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF106">
        <property type="entry name" value="CADHERIN-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 13 14" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 14" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017369497" evidence="17">
        <location>
            <begin position="25"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="556"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="80"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="161"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="270"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="18">
        <location>
            <begin position="429"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="735" mass="81473" checksum="1FFD890A745C0E5B" modified="2018-12-05" version="1">MRIPTTSCLCPFLVCLYFIQRCSGVGHHVPTKVPSKNQTKLASGETEVHHRPKRGWIWNQFFVLEEHIGPDAQYVGKLHSNSDKGDGSVRYILSGEGAGSIFIIDEVTGDIHATKSLDRERKGHYVLHAQALDRNTQEALEPMSEFIIKVQDINDNAPQFPDGPFVATVPEMSEVGTSVLQVTATDADDPTYGNSARIVYSILQGQPYFSVDPKTGIIRTALADMDREAKEHYTVVIQAKDMAGQVGGLSGSTTINVTLTDVNDNPPKFPQKNYQLYVPESAQVGKPVGKIKANDEDLGVNADIKYSIINSEGANMYSISTDRDTKEGIISLKKVSERNYKGIRLVSIGRTWFVYRYQMKGSLDGDDGVRYFRIDESSGVIRTTQPLDREDIPWHNITVMASEVDNPSLLRHVPVTVQVLDMNDNPPEIATNEEVIVCESSKLDQLIQTVTAVDKDDFANGQRFSFALPSQLPVNPNFTLKDNGDSTANIIARRRRFNHLTQELYELPIVVWDGGEPSLSGTSTLTLRVCPCQRHGKIRICQGEAFLSSAGLSTGALIAILLCIVILLAIVVLFVTLKRSKKEPLIIAEEDIRENVVTYDDEGGGEEDTEAFDIIALRNPAAAEELKFRRDIRPDARNHCGMTPCRSPTVELDEVDVHKFIKQRLVEADMDTSVPPYDSLQTYAYEGQGSSTGSISPLDSAGVQSEQEYTYLDDWGPEFQKLAELYEEAESDVTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MD13</accession>
    <name>A0A3B5MD13_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MD13"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021512"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021776.1">
        <property type="protein sequence ID" value="ENSXCOP00000021512.1"/>
        <property type="gene ID" value="ENSXCOG00000016108.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244902"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016501">
        <property type="term" value="F:prostacyclin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048662">
        <property type="term" value="P:negative regulation of smooth muscle cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008365">
        <property type="entry name" value="Prostanoid_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000370">
        <property type="entry name" value="Prostglndn_IP_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY 1 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866:SF7">
        <property type="entry name" value="PROSTACYCLIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01788">
        <property type="entry name" value="PROSTANOIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00856">
        <property type="entry name" value="PRSTNOIDIPR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="32"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="332" mass="37189" checksum="460FA6B23AB35763" modified="2018-12-05" version="1">MNSSSKCHGQHSIAGQGNKPITITFFSLGVVGNLLALFILLVHQKENRSRSSVFCILVTGLVLTDLLGTCLLSPPVFICYAHNMSLIGLGGEKLCNLFGFVMNFFGLASTLILCAMALERCMAISHPYFYSVHIRATFAKVALFFIYLFSLTFCLLPFIGYGKFRQYCPGTWCFMDMGDAPGTWEERGELILAFSLSYSSFNALLIFIVFVCNGSVIVSLCKMHRSHLMRRGSVVSVARKKKLSLAWFGRGEEELDHVVLLALITVIFVVCSLPLTVSIVHPASIQGRVLRDLLRPVRSSRPVFEDQKQKWLLLKKLVVHSVLYPRMFVEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDG9</accession>
    <name>A0A3B5MDG9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">tRNA-t(6)A37 methylthiotransferase</fullName>
            <ecNumber evidence="15">2.8.4.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CDKAL1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 15">Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:37075"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="Rhea" id="RHEA-COMP:11092"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:74420"/>
            <dbReference type="EC" id="2.8.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="15">Binds 1 or 2 [4Fe-4S] cluster. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Endoplasmic reticulum membrane</location>
            <topology evidence="15">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the methylthiotransferase family. CDKAL1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.5" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDG9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021667"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021931.1">
        <property type="protein sequence ID" value="ENSXCOP00000021667.1"/>
        <property type="gene ID" value="ENSXCOG00000016201.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155952"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035598">
        <property type="term" value="F:tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12160:FF:000005">
        <property type="entry name" value="threonylcarbamoyladenosine tRNA methylthiotransferase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.30.20:FF:000002">
        <property type="entry name" value="threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="entry name" value="Methylthiotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006466">
        <property type="entry name" value="MiaB-like_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01578">
        <property type="entry name" value="MiaB-like-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00089">
        <property type="entry name" value="MiaB/RimO family radical SAM methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918">
        <property type="entry name" value="RADICAL SAM PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918:SF45">
        <property type="entry name" value="THREONYLCARBAMOYLADENOSINE TRNA METHYLTHIOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 15" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 15" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="18">
        <location>
            <begin position="59"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="19">
        <location>
            <begin position="195"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="17">
        <location>
            <begin position="426"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="535" mass="60640" checksum="DD50802A85C50B52" modified="2018-12-05" version="1">MPSACDSLIEDIEDMVSFNDPTPHDRQSARKSILPRSRRKKELLSNEELQVDSLIPGTQKIWMKTWGCSHNNSDGEYMAGQLAASGYKITEDPAEADLWLLNSCTVKNPAEDHFRNSIKKAQEQQKKVVVAGCVPQAQPRMDYLKGLSIIGVQQIDRVVEVVDEAVKGHSVRLLGQKKDGGRRLGGARLDLPKIRKNPLIEIISINTGCLNACTYCKTKHARGDLASYPIEELVERARQSFQEGVCEIWLTSEDTGAYGRDIGTDLPTLLWRLVQEIPEGAMLRLGMTNPPYILEHLEEMAKILNHPRVYAFLHVPVQSASDSVLMDMKREYCIDDFRRVVDFLKDKVPGVTIATDIICGFPGETEEDFQDTLELVKHYQFPSLFINQFYPRPGTPAAKTEQVPAHVKKQRTKKLSELFHSYNPYDHKVGERQFVLVTEESFDAQYYVSHNKFYEQVLVPKRADFKGKMIEVDIYEAGKHFMKGRPVEDSKPFTPSIGVPLRKGEVSGLLQVRTRIHTQIHIFKMSPSQVLATTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDH4</accession>
    <name>A0A3B5MDH4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Glycerol-3-phosphate acyltransferase 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Glycerolipid metabolism; triacylglycerol biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027882236.1">
        <property type="nucleotide sequence ID" value="XM_028026435.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027882238.1">
        <property type="nucleotide sequence ID" value="XM_028026437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDH4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021672"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021936.1">
        <property type="protein sequence ID" value="ENSXCOP00000021672.1"/>
        <property type="gene ID" value="ENSXCOG00000016205.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114150161"/>
    <dbReference type="KEGG" id="xco:114150161"/>
    <dbReference type="CTD" id="84803"/>
    <dbReference type="GeneTree" id="ENSGT01030000234574"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07991">
        <property type="entry name" value="LPLAT_LPCAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045252">
        <property type="entry name" value="LPCAT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF10">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063">
        <property type="entry name" value="PHOSPHOLIPID ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="14" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="17">
        <location>
            <begin position="230"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="457" mass="51571" checksum="F5F97AB26A214F42" modified="2018-12-05" version="1">MENLWCVAGTIFQLWMFVVMFFIMLPAMFGLSLGVTEVYIQVLVKILEWATLRIQRGRQEQPSASVPLHKGIIERAGGSMEEEMWQLRCSRPEAAGAFCLSDGVYFCKKGLESIVDDQVTQRFSSEELASWNLLTRTNQNFYYISLRLTIIWGLGVFVRYCILLPFRIALASTGLSWLVIGTSVVGFLPESSAKNWLSELVHLTCYRICARGLSATIHYHNKENRPQKGGICVANHTTPIDVVILANDGCYAMVGQTHGGLLGILQKSMVRSCPHVWFERSEMKDRNAVTSRLRHHIAAKTKLPILIFPEGTCVNNTSVMMFKKGSFEIGGTIYPVTIKYDRRFGDAFWNSSKYNIVSYLLRTMTSWAIVVNVWYLPPMTIQDGEDAAQFANRVKSVIAHQGGLLDLAWDGGLKRYKVKDSLKEEQQKTYSNIIVGQNDIRTTKTCSETRGSITLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDM8</accession>
    <name>A0A3B5MDM8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">EGF-like repeats and discoidin I-like domains 3a</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDM8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022368"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022641.1">
        <property type="protein sequence ID" value="ENSXCOP00000022368.1"/>
        <property type="gene ID" value="ENSXCOG00000016698.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158144"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000002">
        <property type="entry name" value="Coagulation factor VIII"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000226">
        <property type="entry name" value="EGF-like repeat and discoidin I-like domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050633">
        <property type="entry name" value="Neuropilin_MCO_CoagFactor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806:SF9">
        <property type="entry name" value="EGF-LIKE REPEAT AND DISCOIDIN I-LIKE DOMAIN-CONTAINING PROTEIN 3 PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01285">
        <property type="entry name" value="FA58C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01286">
        <property type="entry name" value="FA58C_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="19"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="4">
        <location>
            <begin position="64"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="3">
        <location>
            <begin position="103"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="3">
        <location>
            <begin position="264"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="2">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="425" mass="48197" checksum="80CC632D5E9419EB" modified="2018-12-05" version="1">KCSDIPFFLICYALTALKNNGPCHPNPCHNRGTCEISETYRGDTFIGYVCKCPQGFSGIHCQHNINECEKDPCKNGGICTDQVANYSCECPGEYMGRNCQYKCSGPLGMEGGIISNQQITASSTHRALFGLQKWYPYFARLNKKGLVNAWTAAENDRWPWIQINLQRRMRVTGLITQGAKRIGSPEYVKSYKVAYSDDGKTWRTYKVKGKDEDMIFRGNVDNNAPSANSFTPPIEAQFVRVYPQVCRRHCTLRMELLGCELTGCSEPLGMKSGHVQDYQVTASSIFRTLNMDMFTWEPGKARLDKQGKVNAWTAGHSDQSQWLQVDLLVPTKVTGIITQGAKDFGHVQFVGSYKLAYSNDGERWSVYQDRKQGKDKVFQGNFDNDTHRKNVIDPPVYARFVRILPWSWYGRITLRVEILGCTEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5ME59</accession>
    <name>A0A3B5ME59_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5ME59"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019536"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019776.1">
        <property type="protein sequence ID" value="ENSXCOP00000019536.1"/>
        <property type="gene ID" value="ENSXCOG00000014675.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156443"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19189">
        <property type="entry name" value="PR-SET_PRDM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002548">
        <property type="entry name" value="Zinc finger and SCAN domain-containing protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000512">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044404">
        <property type="entry name" value="PRDM4_PR/SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041493">
        <property type="entry name" value="PRDM4_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF2">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18445">
        <property type="entry name" value="Zn_ribbon_PRDM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="13">
        <location>
            <begin position="404"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="636"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="664"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="691"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="719"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="744"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="772"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="125"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="210"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="532"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="802"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="247"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="532"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="588"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="608"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="802"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="812"/>
            <end position="829"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="861" mass="94001" checksum="AE3611FF7A852191" modified="2018-12-05" version="1">MNDMNLSPVGMDQLSVPSVSASHLGLPTSPTHNPIPTPMPVAIPSLGPSLGSLPSALSLMLPMGPLSDRGVMCGLPERNYSLPPPPYPHLESSYFRHLLPILSYLADRPPPQYIHPSSLNMDGTLSVASNNPSSLDPYSGPGGPLEQGLVSLDSRQVSGQADLHQAGPHELDSTGLAMESRVNSPLSPDRMGEELASMDRVVAVAVSDAQQLGGGRQPQPHEGGVMPLHGPPVLELPVVMEPDHLGGRVGSSGTGGAGGGGAGGGGNGGLGEQLHGNGEMNPSVVSVVLTSSMSNPSQLEPVSLHGHSGMGLEAVNVSSITAEVALGPENNLVLVNSTLQLEDPAPSKENMAAPCSICTLCERSYASDCPEHGPVTFIPDAPIQSRARLSLPRPLCLRISVTDEPLVFARDVIPPRTCFGPMVGQHCSNVDLSDWPEKDTPQIWKMFHNNVLEFYIVTTDENECNWMMFVRKATHEEQNLVAYPANGKLFFCTSTEIHPDQELLFYYSRDYVPQVPDGQICQCGKECSSFSELNSHSSNHSHNQPAHSHSPSLQDHAQTQQQQPAPPQQQQELQSQQHVPPEEKLLNSSSSASSSPWPCSNPAAGQANGDSRSANRNSNSVTSRGRGQGSVREKKYKCSMCSRAFITSTKLNVHFMGHVGMKPHKCEYCSKAFSDPSNLRMHLKIHTQKNYKCTVCGKSFTQKSHVASHMLIHTGAEKLKCDLCDRSFIRKQDLRQHMFSHTQIQCTKCNKHFLKTNHLKKHMNSHEGKRDFVCEKCHKAFLTKYHLTRHLKICKGPKRDRTTRKEQNVFKEEEEEDEDEEEDDDEEEDSRGRRGGDELNDEDYGLDVGGYSSEKSLSPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5ME70</accession>
    <name>A0A3B5ME70_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5ME70"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019546"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019790.1">
        <property type="protein sequence ID" value="ENSXCOP00000019546.1"/>
        <property type="gene ID" value="ENSXCOG00000014659.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158406"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004904">
        <property type="term" value="F:interferon receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015373">
        <property type="entry name" value="Interferon/interleukin_rcp_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050650">
        <property type="entry name" value="Type-II_Cytokine-TF_Rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20859:SF85">
        <property type="entry name" value="INTERFERON ALPHA_BETA RECEPTOR 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20859">
        <property type="entry name" value="INTERFERON/INTERLEUKIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09294">
        <property type="entry name" value="Interfer-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01108">
        <property type="entry name" value="Tissue_fac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="3">
        <location>
            <begin position="8"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Interferon/interleukin receptor" evidence="4">
        <location>
            <begin position="124"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="336"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="401" mass="44410" checksum="1B2ADAE619CB1907" modified="2018-12-05" version="1">QTCSFCRILLWTKKSSNTEAELTPPDDLFMLTLNTNYTLAWDWDQSAAGSDSATFTVQYAGNHSLKKRNPKWTTVCAETSGRSCDLTRSGLNYLGLYVVRVQTNLNGRHSVWKSLQFSPDKDAVLGPPSEVRLGVSGRTLDIFITDPQNSSNSSMKEHLNKLSYDIVYWEQPEDGKTTSTVANVVTLSSIKPWTQYCMQIRSRDDFYNKTSTFTAPLCAQTEGVVPWWKIFMYFLLSLVSCFLMVLLFLYGGHHCFQICKATFLPNEKLPSYIKQVLPSFPTGDYPRLIVSDYESDLCDPVIVCSESAILVINPAVDSAAPPAGLDQDRSSRHSRQNSSSSGDSGVYSAGGNSGGLQPKVASTFTDVNPGFDSEKLNMLSMNSEFKVFPAVPDEGVVDMEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MET4</accession>
    <name>A0A3B5MET4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Myosin IHa</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MET4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022793"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023069.1">
        <property type="protein sequence ID" value="ENSXCOP00000022793.1"/>
        <property type="gene ID" value="ENSXCOG00000017028.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156430"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030048">
        <property type="term" value="P:actin filament-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01378">
        <property type="entry name" value="MYSc_Myo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000101">
        <property type="entry name" value="Slow myosin heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000004">
        <property type="entry name" value="Unconventional myosin ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036072">
        <property type="entry name" value="MYSc_Myo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF852">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IH ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myosin motor" evidence="10">
        <location>
            <begin position="48"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="11">
        <location>
            <begin position="865"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="9">
        <location>
            <begin position="599"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="141"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1040" mass="119696" checksum="76FE02CDF1D3AA7D" modified="2018-12-05" version="1">LLCPSMGPHMSKVMESKLQHKIAKNKALLFALGGRLDMEGALTTRDRVGIQDFVLLDETTEVAIISNLKKRFSKDLIYTYIGTLIVSVNPYKDLDIYNKKQMDVYMGVNFFELPPHIYALADNAYHTMLTEFNNHFILISGESGAGKTEASKKILQYYAVSCPSTTLMNTVRDKMLMSNPVLEAFGNAKTLKNDNSSRFGKYMDIQFDSQGDAVGGHILNYLLEKSRVVHQNHGERNFHVFYQLVEGGSDDLLTQLGLKRDVQHYYYLTQGECAIVSSINDKNDWKSVKNALQVIEFDETNTNHLFRVVASVLHLGNVHFDPDSKGHALLKNNTELNWVSDLLGVDANNLKEGLTFRKIEAKTEQVLSPFTIDHAIYVRDALAKAIYGQTFTWLVNRINESMENKDSPRKTVIGLLDIYGFEVFYVNSFEQFCINYCNEKLQQLFIQLTLKAEQEEYEAEGIEWEPVQFFNNKIICDLVEEKHRGIISILDEECLRPGDATDLTFLDRLEEKMGNHPHFVTHRLADKMTRKTLERGDFRLLHYAGEVTYCVVGFLDKNNDLLYRNIKNVICLLQECFSAVDTTNKRRPETVATQFKNSLQKLTEMLMAKEAWYIRCLKSNESKQPGQFDEALIRHQVKYLGLMEHLRVRRAGFAYRRKYEDFLKRYKPLCPATWPHWRGMPADGVEVLAQHLGYLPDEYKMGRTKIFIRHPRTLYATEDAYEKCKHDLGNASKLNFNFMSSATKIETCWRGAQARKEKEKRAWAVKVIKKFIKAYINRGEAKSTDNSEYLAFVRQSYLNRLKNNLPKTVLDKTTWLTPPAVLTQASEILRKLHYRLMVRKYVRGIPPQKKLQMKVVTSAIFKGKKENYPPSIPQLFLDTRISEQEINIRVLSMIRNEQVKYSVPVIKYDRNGFKPRPRQFILTKTAAYLVEEAKIKQRVSYNSLKGISVSNLSDGIIVIHITCEDPKQKGDLIIQCEHLYEFLTKLSIIANKQDAIKVVQGSIKIQIQSGKENSVDFTTGQEPMVYKAKNGHLMVVSINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEW1</accession>
    <name>A0A3B5MEW1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEW1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022823"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023099.1">
        <property type="protein sequence ID" value="ENSXCOP00000022823.1"/>
        <property type="gene ID" value="ENSXCOG00000017054.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00500000044936"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001965">
        <property type="term" value="F:G-protein alpha-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042651">
        <property type="entry name" value="Rgs22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46583">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46583:SF1">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="2">
        <location>
            <begin position="435"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="2">
        <location>
            <begin position="712"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="851"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="857"/>
            <end position="868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="889" mass="103824" checksum="B0DAE9608B626135" modified="2018-12-05" version="1">FLSLPSFSEALVYNQQSGLFEVVSGKADIIAKQISATLKLYKSILLSDDPKAFSKMPPVPDICFAVVCLHKEEGIQWIKEKRLPFFLQSDCYHEYRLTKLLFQWKPTFWSHDLKNWLTTNVSDVKSCPSHTHSKTRSFSFFSLSSSGVECEQTQELHSSSDLSDNLTAEEQPEYFASKVVNQVLKEAVDVLDGQKQAETSESLSQPDLSRTDDQHEANKDEAKPERKKSAPLNSAKNSRDKPEEVKKSPVLCWEVSSAENRPGLDEFKEFLRGTSGEKLLNLWMDIQTLIALQTEERKKRYLVLLRNCYLRNSSPCCLNGELLARLGLSNSPCWIEEKLFSVQELLTEPLLSYWCGPQFRFIFAMSPVPVSIQPINLHTSSICPTGYLDLNLHFYKVAYTVVYIFPKMSAKCETPFGRRVKPMMTSLFIEFQTGLYFTQYCEKSGNKLWVNAVYFWTDLQHYHELFHQEQMDPYSVQREAQVLYSTYISSFARRNINVKTETRREVYNKLRPAFEELFDRVEEHALDILLEPWTQLNQRDKESFLQVRGSTKQRGGIYRKMRFIQRFNFFLFLCSIHLLCWLDLERYKAIFKGNKRARLEKTTLIVKKYLNEDYFFGPDSPATKEQQEDVFKAGGEEKLQSESLSNIVVIAIQEIIRRYLEETWVPLFLSSKEFLERQKIKEREKNLLTKKQTKRNSMTVGSVMSTSRDILLFRRALLNPSTCKQFQDYVSVKGELLENDVRFWVEVQRYKDLCHSHSNLTIIQQKISTIINCFINSSMPPSVQINISHEQARRILERRRELGPYIFREAQIAVFSELLKLWPKFQVLSRNLSKAELIPLLQEKRAKHKAKIRKQRRLEEEESEKKAQVDPNKQTNKTEVIRNQIVLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MF68</accession>
    <name>A0A3B5MF68_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Purine-rich element binding protein Aa</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PUR DNA-binding protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027887406.1">
        <property type="nucleotide sequence ID" value="XM_028031605.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027887407.1">
        <property type="nucleotide sequence ID" value="XM_028031606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MF68"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023215.1">
        <property type="protein sequence ID" value="ENSXCOP00000022938.1"/>
        <property type="gene ID" value="ENSXCOG00000017141.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114153212"/>
    <dbReference type="KEGG" id="xco:114153212"/>
    <dbReference type="CTD" id="415106"/>
    <dbReference type="GeneTree" id="ENSGT00950000183162"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032422">
        <property type="term" value="F:purine-rich negative regulatory element binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.700:FF:000001">
        <property type="entry name" value="Purine-rich element binding protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2450.30:FF:000001">
        <property type="entry name" value="Purine-rich element binding protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2450.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006628">
        <property type="entry name" value="PUR-bd_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12611">
        <property type="entry name" value="PUR-TRANSCRIPTIONAL ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12611:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN PUR-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04845">
        <property type="entry name" value="PurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00712">
        <property type="entry name" value="PUR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="285" mass="31874" checksum="ADAE1E86809E8F41" modified="2018-12-05" version="1">MADRDSGSEQGGAATGPGFGSMHLAAGGAGSASGLQHETQELASKRVDIQNKRFYLDVKQNAKGRFLKIAEVGAGGNKSRLTLSMSVAVEFRDYLGDFIEHYAQLGPSNPGMVQDEPRRALKSEFLVRENRKYYMDLKENQRGRFLRIRQTVNRGPGLGSTQGQTIALPAQGLIEFRDALAKLIDDYGVEDEPAELPEGSSLTVDNKRFFFDVGSNKYGVFMRVSEVKPTYRNSITVPYKVWSKFGNTFCKYAEDMKKIQEKQREKRACELQQQEEMQADDGDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFI6</accession>
    <name>A0A3B5MFI6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Myosin VC</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MFI6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022360"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022633.1">
        <property type="protein sequence ID" value="ENSXCOP00000022360.1"/>
        <property type="gene ID" value="ENSXCOG00000016692.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157971"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000002">
        <property type="entry name" value="Class V myosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000089">
        <property type="entry name" value="Myosin VC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF313">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-VC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myosin motor" evidence="10">
        <location>
            <begin position="41"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="135"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="691" mass="78544" checksum="71B8B5664B4A6A36" modified="2018-12-05" version="1">KDFHSGDNVLELLLEDGTVHLHPVDPSKPKLPPLRNPDILVGENDLTALSYLHEPAVLHNLKVRFVESRIIYTYCGIILVAVNPYKQLPVYGDAIIHAYSGQNMGDMDPHIFAVAEEAYKQMARNHKNQSIIVSGESGAGKTVSARYAMRYFAVVSKSGSQTRVEDKVLASNPITEAIGNAKTTRNDNSSRFGKYTEISFDRRYRIIGANMRTYLLEKSRVVFQAENERNYHIFYQMCSCADSAEFKHLRLLSADEFRYTGMGGDITIDGVYDKKDFEETRQTFSLLGLKEDFQSDIFKVLAAILHLGNVEIRSSGDDKSSAAVSLSDPHLAAFCDLLCVSAAELVRWLCNRRIVLVAETVVKPVPKERAAAGRDALAKQIYAHLFDCIITRINTALQVPGKQHAFIGVLDIYGFETFDINSFEQFCINYANEKLQQQFNLHVFKLEQEEYMKEDIPWTLIDFYDNQPVIDLIEAKMGILDLLDEECLFPQGTDQSWLQKLFNYLEANPLFEKPRLSNEAFVIQHFADKVEYQCRGFLEKNRDALYEELVDTMRASKFSFLAHFFQEEEQSRAASKGVKVKSARPSVKPANKQLRTSVGDKFRSSLSLLMGTLNIVKILNSYTPIDDFEKRVTSSFVRQVQSFLQEYEGATQLMLDTDYRFQVTFPFCPSSTALESLQVPSSLHLEFLTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFJ8</accession>
    <name>A0A3B5MFJ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RAB1A, member RAS oncogene family b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027862003.1">
        <property type="nucleotide sequence ID" value="XM_028006202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MFJ8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018379"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018611.1">
        <property type="protein sequence ID" value="ENSXCOP00000018379.1"/>
        <property type="gene ID" value="ENSXCOG00000013842.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114137547"/>
    <dbReference type="KEGG" id="xco:114137547"/>
    <dbReference type="GeneTree" id="ENSGT00940000155078"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01869">
        <property type="entry name" value="Rab1_Ypt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000069">
        <property type="entry name" value="Ras GTP-binding protein YPT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057289">
        <property type="entry name" value="Rab1/Ypt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="174"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="22391" checksum="C06BBB7043F78084" modified="2018-12-05" version="1">MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNASNVEQAFMTMAAEIKKRMGPGATAGGGEKPNVKLTPGTTVKPSPGGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFV6</accession>
    <name>A0A3B5MFV6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Annexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Zymogen granule membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">A pair of annexin repeats may form one binding site for calcium and phospholipid.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the annexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MFV6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020248.1">
        <property type="protein sequence ID" value="ENSXCOP00000020001.1"/>
        <property type="gene ID" value="ENSXCOG00000015010.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155988"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042589">
        <property type="term" value="C:zymogen granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000001">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000002">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000003">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.10:FF:000004">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.10">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001464">
        <property type="entry name" value="Annexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018502">
        <property type="entry name" value="Annexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018252">
        <property type="entry name" value="Annexin_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037104">
        <property type="entry name" value="Annexin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002391">
        <property type="entry name" value="ANX4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10502:SF135">
        <property type="entry name" value="ANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00191">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00196">
        <property type="entry name" value="ANNEXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00200">
        <property type="entry name" value="ANNEXINIV"/>
    </dbReference>
    <dbReference type="SMART" id="SM00335">
        <property type="entry name" value="ANX"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47874">
        <property type="entry name" value="Annexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00223">
        <property type="entry name" value="ANNEXIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51897">
        <property type="entry name" value="ANNEXIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 10" id="KW-0041">Annexin</keyword>
    <keyword evidence="4 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 10" id="KW-0111">Calcium/phospholipid-binding</keyword>
    <keyword evidence="8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="316" mass="35116" checksum="817C69C51BE7EF59" modified="2018-12-05" version="1">MFILFPCFCLQEYRGSVRPFVHFNAKHDAEFLHKAMKGIGTDEDAILMVLTARSNDQRQQIKAAYKKAYGKDLVSALKSELGGLFEALIVALMTPPVLYDATLLHKALKGAGTDDQVLIEILASRTGDEIKDISKVYKKEFGGKLEKDISSDTGGSYGRLLVILLQVCALPLQDLYAAGEEKFGTDEEKFITILGNRSSEHLQKVFDAYKKISGSDIEDIIKGETTGNLEDLLLAVVKCVRNVPGFFAESLYQSMRRAGTDDDTLMRIMVSRSEVDMLDIRANFKKMYGQSLYTAIQEDTAGDYQKALLYLCGGKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGT4</accession>
    <name>A0A3B5MGT4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">D(1B) dopamine receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">D(5) dopamine receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Dopamine D5 receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGT4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022952"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023229.1">
        <property type="protein sequence ID" value="ENSXCOP00000022952.1"/>
        <property type="gene ID" value="ENSXCOG00000017153.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157037"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001588">
        <property type="term" value="F:dopamine neurotransmitter receptor activity, coupled via Gs"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071880">
        <property type="term" value="P:adenylate cyclase-activating adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000045">
        <property type="entry name" value="D(1A) dopamine receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000497">
        <property type="entry name" value="Dopamine_D5_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000929">
        <property type="entry name" value="Dopamine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF136">
        <property type="entry name" value="D(1B) DOPAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00566">
        <property type="entry name" value="DOPAMINED1BR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00242">
        <property type="entry name" value="DOPAMINER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 16" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 16" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="18">
        <location>
            <begin position="30"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="429" mass="48444" checksum="6B42C8A40C70B7BD" modified="2018-12-05" version="1">MNRSTGKIDVVVRTVTGCLLSLLILWTLLGNILVCSAVLRFRHLRTKVTNIFIVSLALSDLFVAVLVMPWKAVAEVAGYWPFGTFCNVWVAFDIMCSTASILNLCIISVDRYWAISSPFRYERKMTQRVAFVMISITWTLSVLISFIPVQLNWHKATADGLAGAHNSSASRQLEENCDSSLSREYAISSSLISFYIPVAIMIVTYTRIYRIAQIQIRRIASLERAAEHAQSCRSNRIECQHHNTLKTSIKRETKVFKTLSVIMGVFVCCWLPFFVLNCMVPFCNKPAADRDAGLPCVSETTFDIFVWFGWANSSLNPIIYAFNNEFRKAFASLLGCRHFCSSTPVETVNISNELVSYNQDTIIHKEIANAYVNMIPNVVECMEHEETFDRISQFSHNNDNATDSVCDLEDCEADISLDRLSPFTPNGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGU9</accession>
    <name>A0A3B5MGU9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">14-3-3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homodimer, and heterodimer with other family members.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 14-3-3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGU9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022840"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023116.1">
        <property type="protein sequence ID" value="ENSXCOP00000022840.1"/>
        <property type="gene ID" value="ENSXCOG00000017069.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01090000260040"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023409">
        <property type="entry name" value="14-3-3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00797">
        <property type="entry name" value="1433_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="3">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="120" mass="13625" checksum="B18E5B2B30D371B4" modified="2018-12-05" version="1">MYVQLSNCIIKCLKLGIIKDSQKAYQQAFDISKAEMQPTHPIRLGLALNFSVFFYEILNSPEEACKLAKQAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDNAVEGEESEEPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MH85</accession>
    <name>A0A3B5MH85_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MH85"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023122"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023400.1">
        <property type="protein sequence ID" value="ENSXCOP00000023122.1"/>
        <property type="gene ID" value="ENSXCOG00000017276.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182984"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000037">
        <property type="entry name" value="Serine/threonine-protein kinase tousled-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974">
        <property type="entry name" value="MIXED LINEAGE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974:SF20">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE TOUSLED-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="376"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="24"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="277"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="664"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="327"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="42"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="303"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="686" mass="79027" checksum="841B2B0142CD1A01" modified="2018-12-05" version="1">MMEELHSLDPRRQELLEARFTGVGVSKNESSNQSLCSLGSLSDKELETPEKKANEQRVRKRKADHFDSSHGKAGARGLKISDYFEVSVFFLCTFLSHQNKTSSLNYVSSSQVQQGSPSSISSVNTELSTCAMKPVVLHMLHKTTQSDLTLEKLTALENSKNSDLEKKEGRIDDLLRANCELRRQIDEQQRMLERYKERLNKCVTMSKKLLIEKSKQEKMACRDKSMQDRLRLGHFTTVRHGASFTEQWTDGYAFQNLIKQQERINSQREDIERQRKLLAKRKPPSMAQTPPPSLEQNKRKSKTNGTESETLSQAEYHEQEEIFKLRLGYLKKEEAEIQAELERLERVRNLHIRELKRIHNEDNSQFKDHPTLNDRYLLLHLLGRGGFSEVYKAFDLTEQRYVAIKIHQLNKNWRDEKKENYHKHACREYRIHKELDHPRIVKLYDYFSLDTDSFCTVLEYCEGNDLDFYLKQHKLMSEKEGRSIIMQIVNALKYLNEIRPPIIHYDLKPGNILLVNGTACGEIKITDFGLSKIMDDDSYNSVDGMELTSQGAGTYWYLPPECFVVGKEPPKISNKVDVWSVGVIFYQCLYGRKPFGHNQSQQDILQENTILKATEVQFPPKPVVSPEAKAFIRRCLVYRKEDRIDVHQLASDPFLMPHIRKSVASSGSSGTAVASTSSSSNSSASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIQ1</accession>
    <name>A0A3B5MIQ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 186</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM186 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIQ1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019434"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019674.1">
        <property type="protein sequence ID" value="ENSXCOP00000019434.1"/>
        <property type="gene ID" value="ENSXCOG00000014600.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000087"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026571">
        <property type="entry name" value="Tmem186"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045325">
        <property type="entry name" value="TMEM70/TMEM186/TMEM223"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13603">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13603:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06979">
        <property type="entry name" value="TMEM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="219" mass="24998" checksum="AD9A61170EF93052" modified="2018-12-05" version="1">FQRSTTPIFLTRFTSQALACTRRSFLLSRYQPRSAHTPFLQSCHGTLTPEITTLIRYSDLSAQKYSMIYTLPHIRLLRAMSRLKLFQTAVTLVLLPPVYFLYFQGIASLFLVSYSTGIALFAGGMLYIASHLFRRVVGMMYLDPSLTTLKVSHLTFWGKRQDIYLPVSDVMTIADTGDSASETILKLKRYSSPDTFYFSTRFGRVVNKQGFEKVFGSFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKC7</accession>
    <name>A0A3B5MKC7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IF rod domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKC7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019974"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020221.1">
        <property type="protein sequence ID" value="ENSXCOP00000019974.1"/>
        <property type="gene ID" value="ENSXCOG00000015000.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159820"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF32">
        <property type="entry name" value="PHAKININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="5">
        <location>
            <begin position="99"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="150"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="272"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="395" mass="44113" checksum="38EF9E1572D83DD7" modified="2018-12-05" version="1">MPLPRRHSSFLGQSSSERPAAASGGRTSAAGATMPRGTPVGPGATSGLGTRVSRRALGISSVFLQGMRSCTAPVLPRAGDRTAGGGTTGLNSCLMEYRDKVRALEELNRQLEQQIRLSLDRKAASAGAWGPLRREWEDIYRQVSEAILENARLMLQTENVQANAEDFKERLEPEQNLTVVEDEVGSLRRVMEEAAVTKVELEERMESMRAELLHLESRHEQDVRALYSRTAGRDVDEPDTPVETGLDRILAHIRTHWEQLTERNRADTDGYLESKVSHLSAEEQQAEALKAECSDVSCRIQSLQAETESIRALKRGLENSLSDAHHWHDVELQNLGSVVARLEAELSDVRSEIEQQRRDFDMLLSNRQRLEQQIGLYHGILDGEEDRFLPSNAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKQ8</accession>
    <name>A0A3B5MKQ8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Septin 5b</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKQ8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021726"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021990.1">
        <property type="protein sequence ID" value="ENSXCOP00000021726.1"/>
        <property type="gene ID" value="ENSXCOG00000016246.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159913"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000064">
        <property type="entry name" value="Septin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="10">
        <location>
            <begin position="26"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="324"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="site" description="Important for dimerization" evidence="7">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006698-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="357" mass="41176" checksum="30A9619071744FBF" modified="2018-12-05" version="1">MSAGIRLQYVGFATLPNQLHRKSVKKGFDFTLMVAGESFGLGKSTLVNSLFLTDLYKERKLLNAEERIKQTVEITKRTVDIEEKGVKLKLTIVDVPGFGDAVNNTECWTPVTDYINQQFEQYFRDESGVNRKNIQDNRVHCCLYFISPFGQGLRPIDVEFMKALQDKVNVVPLIAKADCLTPSEIKRLKEQVREEIDKFGIKIYQFPDCDSDEDEEIKQQDKGLKESLPFAVIGGNTVLEVRGQRVRGRLYPWGIVEVENPSHCDFGKLRNMLIRTHMHDLKDTTNDCHYENYRAQCIQNMTSKMNADKRVESPIPILPLSTPAVETEKLIKRKDEELRRMQQMLQKMQQQMHGQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLU9</accession>
    <name>A0A3B5MLU9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLU9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022390.1">
        <property type="protein sequence ID" value="ENSXCOP00000022121.1"/>
        <property type="gene ID" value="ENSXCOG00000016533.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162312"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd16040">
        <property type="entry name" value="SPRY_PRY_SNTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="3">
        <location>
            <begin position="154"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="350" mass="38632" checksum="03D4B0E239363492" modified="2018-12-05" version="1">SVNFLLSDQIQDLHLIWDSFSGTGAKHDPFYKERTEEDPEGFESMLSGCNLSARSCEALSSVLGSQSSSLRDLDLSNNDLEDSGIKHLCHGLESPCCKLETLSLSGCLISEKGCSSLALALTSNPSHLRELDLSYNHPGEAGLELLSVSLKMPDCRLDKNSRNKQDKRTQDFCQLTLDPNTAHKFLRLSENNRKVTDLRKEHPYPDHQERFDYWPQVLCETGLSGRCYWEVEWVGKVYVAVSYRGIGRNGDSDGSKFGGNDQSWCLACTDGGYSAWHNNSGSPIPLPPSPTASHRVGVYVDGPAGILSFYRVSGDVVTHLHTFSTTFTEPLYAGFGYGVKFNSSALLCQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MN60</accession>
    <name>A0A3B5MN60_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Limb and CNS expressed 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LIX1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LIX1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MN60"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022591"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022867.1">
        <property type="protein sequence ID" value="ENSXCOP00000022591.1"/>
        <property type="gene ID" value="ENSXCOG00000016884.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005869"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051436">
        <property type="entry name" value="Autophagy-related_EPG5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029270">
        <property type="entry name" value="LIX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31139">
        <property type="entry name" value="ECTOPIC P GRANULES PROTEIN 5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31139:SF5">
        <property type="entry name" value="PROTEIN LIMB EXPRESSION 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14954">
        <property type="entry name" value="LIX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="354" mass="39748" checksum="B06FF02D67587710" modified="2018-12-05" version="1">FILKGMNGAQPLNSAGSDLSVKSKMSQPPLVLDTDEWTLVPLMPAYSSTKISKSTGSVMFFLLELSFFFPSENSSTAQYSLLLYESAPPPGPPYVCYVTLPGGSCFGNYKVCDTQAEARRDAARVALMNSLVNEFPCRRITAEFITHSLNQAATESAVSTSIGTYSLLLQSYMGRTMLEFQEMMTVFQLLHWNGTLKALREKQCSRQSVITYYSERGLDESMRSSMALDWLGREQKSPGLLAVELQMAQRELVLARRRGIELRFYKEKTEILSLALSQAYIHHPPGVFSPTPSHTDQHEQLSLHVLYSQDTDDQMSQHTVFNNAMQTSTDTPANSPLLFSQCMFMPLYNPKYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNE3</accession>
    <name>A0A3B5MNE3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ZZ-type zinc finger-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Histone H3 reader that is required for the ATAC complex-mediated maintenance of histone acetylation and gene activation. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the ADA2A-containing complex (ATAC), composed of KAT14, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1. Interacts via (ZZ-type zinc finger) with histone H3 in a methylation-independent manner and acetylation on 'Lys-4' (H3K4ac) moderately enhances the interaction.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNE3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025929"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026240.1">
        <property type="protein sequence ID" value="ENSXCOP00000025929.1"/>
        <property type="gene ID" value="ENSXCOG00000019369.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005307"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140672">
        <property type="term" value="C:ATAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051302">
        <property type="term" value="P:regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02341">
        <property type="entry name" value="ZZ_ZZZ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000128">
        <property type="entry name" value="Putative ZZ-type zinc finger-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037830">
        <property type="entry name" value="ZZZ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041981">
        <property type="entry name" value="ZZZ3_ZZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22705">
        <property type="entry name" value="ZINC FINGER, ZZ DOMAIN CONTAINING 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22705:SF0">
        <property type="entry name" value="ZZ-TYPE ZINC FINGER-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HTH myb-type" evidence="19">
        <location>
            <begin position="654"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="17">
        <location>
            <begin position="654"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="18">
        <location>
            <begin position="824"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="201"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="307"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="610"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="217"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="318"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="345"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="363"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="428"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="447"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="472"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="613"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="909" mass="101721" checksum="7C883EDFDC3A0F58" modified="2018-12-05" version="1">MAAPRSSRVTRSSVGLNGLDEKFCGRTLRNRSIAQPGETSVSPQPRARSPKKKQDTNKDNRQESKNIADSSIPAGDEDHSTVSRKRGLSRLEKDLKQEKSENCDTGKEWRNTSPPIKRAKQCSLSGEFLGHEEDQGFLNPQSQVSVSEPCKDLICEDLPSHNSCNAPPASSILSEHEGEVVGRQEKEDYCLDCDGPVFPQNSHKASNGLRETDAEETSTVTEYPSETPTSATSFPSLLNGSQMEAPSSPDSSVPFRNSVAGQMAVSGSGESPASSALTFMGSVEDPVPELIVAKEQEVEEVEVDVVGDPLGLAHEEQVTVSEGDTNGRSPSPVQEPAASSSNSGETMPTPTTPQSPTEPECNPSMSSMTPSFTELNEHRYTLRTSPRRVVSGGKASPSKPCSPMTDNGPFREEGEVEDDCPMLEEPSCSDSVGLSSEPGSVNPSCRASEKDSDFMEDKETENGKELTRSEAVEEEEEEEEEEEEEEEEEEEEPDVYYFESDHLALKHNKDYQRLLQTIVVLEAQRAQAILDLETLARQQREALSEPISFVEQLQKQVTLGLPRPQRVVQLPDIAWEQYTSGLGEFEREFCDKKRKTRQLKLIFDKGLPLRPKSPVEPKKEGESSAVYSSLPTSDAPENGRQTQMIRGRICHPNKPDTFNQLWTVEEQKKLEQLLVKFPPEEVESKRWQKIADELGNRTAKQVASRVQKYFIKLTKAGIPVPGRTPNLCMYTKKASSKRQHHLNKHLYRPSTFLTSYEPPVYMEDEDERAAFYHSVQDPSADDSDEESVPVELRNLQEYKELLQLKRLKKQKLQEIREDNAGVQHMGYKCDVCGMDPIQGVRWHCQDCPPENSVDFCSNCSDCLFKTETHKPDHHLEAVYQPNTFLDRDYCLPQSAGYNYLDPNYFPANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNZ1</accession>
    <name>A0A3B5MNZ1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Si:ch211-244c8.4</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Amer family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNZ1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023152.1">
        <property type="protein sequence ID" value="ENSXCOP00000022876.1"/>
        <property type="gene ID" value="ENSXCOG00000017090.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01130000278440"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060828">
        <property type="term" value="P:regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019003">
        <property type="entry name" value="AMER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22237:SF3">
        <property type="entry name" value="APC MEMBRANE RECRUITMENT PROTEIN 2-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22237">
        <property type="entry name" value="APC MEMBRANE RECRUITMENT PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09422">
        <property type="entry name" value="AMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="126"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="305"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="374"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="174"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="206"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="418"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="527"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="538" mass="56728" checksum="2C09E7F951DB888B" modified="2018-12-05" version="1">MDVQTENTDPPPNESQPSGKIRKGFKLFGKRKPGSIFSIRNKGDGNNKSPVIRSKTSEGLYETPAQDSEQEQDKENGKDVKQEDGEQPEEEALGEDGVLATAPARTSISSASSAKSLGFLSLLRGGKRGVGDRRVHTVSQPVARQRRGLKGLFSNVKLRSKDKEDKEEAPPSPLLQSSRTNSVEIIKEDMTLTPKCQPRALDSPETESTGPVQSSATPNSSAMTPSKTSATQVTSAENVSTTYEHVSPLLTPEPPLVPGESSLSSLLADISSLLTFDSISGGGDIMADVEAEWGKVSSAISADIPPSSTSPFFKSTLSSSPTTSSTVSSAATVKASSVMLPAQPATAAAPTHVASLSSLTPSAVDQMKVSPAVTSIPVSKELSAPTHLPPKEKKTPQSKAMGLSKIPVVGGGKVSRQPVRENQHGEPSKDLTSPVLEEKPYFNSHNTGGKDKISSPEAIGLISKQLQEEHQQPQQAKVPTSSARDSKIPLKHGSGTHAASQVSQAKEPPRTKIPVSKVPVRRAGNKPATTGSSTQIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPE4</accession>
    <name>A0A3B5MPE4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 20">Protein kinase C</fullName>
            <ecNumber evidence="4 20">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="4 20"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MPE4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023036"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023313.1">
        <property type="protein sequence ID" value="ENSXCOP00000023036.1"/>
        <property type="gene ID" value="ENSXCOG00000017214.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155327"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20834">
        <property type="entry name" value="C1_nPKC_theta-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20837">
        <property type="entry name" value="C1_nPKC_theta-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000360">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000003">
        <property type="entry name" value="Protein kinase C delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000049">
        <property type="entry name" value="Protein kinase C delta type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000008">
        <property type="entry name" value="Protein kinase C theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000150">
        <property type="entry name" value="Protein kinase C, theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014376">
        <property type="entry name" value="Prot_kin_PKC_delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21494">
        <property type="entry name" value="PKC_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000551">
        <property type="entry name" value="PKC_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 20 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 20 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 20" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="26">
        <location>
            <begin position="160"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="26">
        <location>
            <begin position="232"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="332"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="27">
        <location>
            <begin position="587"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="24">
        <location>
            <begin position="303"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="21">
        <location>
            <position position="456"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <begin position="338"/>
            <end position="346"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22 23">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRNR" id="PIRNR000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000551-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000551-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="659" mass="75149" checksum="0C1EB79253E06C49" modified="2018-12-05" version="1">MAPFLRFTLQGYDMGALGPVDGTPTCAVRIKESVSTERGKTLVQKKPTMYLQWKSSFDAHIYEGRVIEVILLKGSDEPLAKVTLGMSTLTERCKALKNKNQTEFWVELHPSGRIQISLQHFLEDTDIGKKKKKTSALEGVMTLNRRRGAVKQQKIHGIKNHEFIATFFRQPTFCSVCREFVWGLNKQGYKCKQCNAAIHKKCFEKIIYNCTGTAANSVDTMFQSERFNVNVPHRFKFYNYKSPTFCDHCGSLLWGLVRQGLKCDACNMNVHSYCQVKVANMCGVDQKLFAEVLAQFATKPKSVRPPPPATTPAQDPTPAPTSQLLHLKLDNFILHKVLGKGSFGKVMLAELKGSGLYFAVKALKKDVVLMDDDVECTMVEKRVLSLAWNNPFLTHLYSTFQTKEHLFFVMEFLNGGDLMFHIQEKGRFDLTRATFYAAEIVVGLQFLHAQGIIYRDLKLDNVMLDSSGHIKIADFGMCKENISAENKTGTFCGTPDYIAPEILVGQKYTFSVDWWSFGVLVYEMLIGQSPFHGDNEDELFESIRLDAPNYPKWITDESRSLLEQLFVREPSRRLGVVGDIRAHSFFQLINWSALENRQVEPPHKPKVKSPNDYSNFDRDFLSEKPRLTNCDKKLLETMDQTAFRDFSFVNPKMEQLIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPS9</accession>
    <name>A0A3B5MPS9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcriptional adapter 3-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NGG1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MPS9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023181"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023459.1">
        <property type="protein sequence ID" value="ENSXCOP00000023181.1"/>
        <property type="gene ID" value="ENSXCOG00000017323.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008947"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019340">
        <property type="entry name" value="Histone_AcTrfase_su3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13556:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13556">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10198">
        <property type="entry name" value="Ada3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="71"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="207"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="271"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="207"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="367" mass="41773" checksum="2F135E3CEAC2C1D3" modified="2018-12-05" version="1">MSELKDCPPLKYYDFKPVEHVKLCPRYTAVLGRSEDDGIGIEELDTLQLELETLLSSASRRLRALEEQRQILTDWQDKKGDKRFLKPSKDPDPAAPVRHKPKKQKLDCKGGHGPGPGPGRPKSKNLQPKVQEYEFTDDPQDIPRTPKNDAPNRFWASVEPYCADITNEETRLLEELLKPPEDEAEYFKIPALGKHYSQRWAQEDLLEEQREGARANDKKKSIMGGPLSELDAKDVDSLLKKSESQHESPEDGCPFGPLTQRLLQALVEENIISPMEDSPIPDMSGKDANDGAGTSPRSQGKAFRLAKEEMRKQELRQRVRVADNEVMEGFRRIMAARQKKRTPTKKEKDQAWKALKERESILKLLDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MR25</accession>
    <name>A0A3B5MR25_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Metalloendopeptidase</fullName>
            <ecNumber evidence="9">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8 9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MR25"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023646"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023929.1">
        <property type="protein sequence ID" value="ENSXCOP00000023646.1"/>
        <property type="gene ID" value="ENSXCOG00000017421.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161051"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04283">
        <property type="entry name" value="ZnMc_hatching_enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000038">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034039">
        <property type="entry name" value="ZnMP_hatching_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF899">
        <property type="entry name" value="ASTACIN-LIKE METALLOENDOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 8 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5 8 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="10">
        <location>
            <begin position="81"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="295" mass="33911" checksum="F5CB0ABEAFE591D7" modified="2018-12-05" version="1">SSIFFVLNKFKNVIFHMFCCNHNYSFPYFPESVFCFTGNNIEDDDFSVSTLLEKANANLGENLDDPLVMFGDIAVPTGLQNADPCTARGCLWNKDQDGNVYIPYSISNQFSQRERSIIVRGLESFHGSTCLRFRLRQRERDFVDIQSRSGCWSFVGRIGGGQVVSLSRNGCVFHQIVQHELLHALGFNHEQTRSDRDSHVRILLENVIRGQEHNFRKIDTNNLGTPYDYNSVMHYGRFAFSRNREPTIVPIPDPNVEIGRATEMSQNDILRVNRTCLSTNQLDLVRKNRTNLTRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MR95</accession>
    <name>A0A3B5MR95_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">THAP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MR95"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024000.1">
        <property type="protein sequence ID" value="ENSXCOP00000023716.1"/>
        <property type="gene ID" value="ENSXCOG00000017712.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164656"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.20">
        <property type="entry name" value="THAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006612">
        <property type="entry name" value="THAP_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038441">
        <property type="entry name" value="THAP_Znf_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027805">
        <property type="entry name" value="Transposase_HTH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23080:SF133">
        <property type="entry name" value="SI:CH211-262I1.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23080">
        <property type="entry name" value="THAP DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13613">
        <property type="entry name" value="HTH_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05485">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00692">
        <property type="entry name" value="DM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00980">
        <property type="entry name" value="THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50950">
        <property type="entry name" value="ZF_THAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="THAP-type" evidence="8">
        <location>
            <begin position="22"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="452" mass="51229" checksum="C8830F1E638007FA" modified="2018-12-05" version="1">FLSVATMKRMQKIKYLRATGTTTEFCCVPLCPVSSRCNKFLSFFSFPADEELRKQWIVAIRRADLAIKAHTRVCSRHFKPEDIKEPEKEMGRRRLKKGAVPALFEWNNFSLPLPPLGVCEKRIRPPPEDGEEEANPPANVCTKEHDYASAPDPAVVDLVLEENYALWEEICQLREQAESLTLRQRFGIHRFAGTAIDSSTQTLQPIDEFFLFLNYLALGSKQRDLADRYGVHLSTVSWIITAWTNFLFTMLGSIRIWIPEDQIHSSLPADFKDYPDTTVILDCIELRCQCPSSPILFCKMFSSYKSHCTLKGLIGIAPHGAVTFISGLYAGSISDKQITRESGLLSLLKPGMAVMVYRGLIIDDIVPCKVYRPAFFFGRSQMSAGEVRETPATARLGVHVETLIHRVKEHKLFDSEIPLCVLGIINQLYTVACLSNFISLSFLEKKLQKYFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MTT6</accession>
    <name>A0A3B5MTT6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ras-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MTT6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027824"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028159.1">
        <property type="protein sequence ID" value="ENSXCOP00000027824.1"/>
        <property type="gene ID" value="ENSXCOG00000020748.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183076"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903479">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.506.10:FF:000004">
        <property type="entry name" value="IQ motif containing GTPase activating protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000593">
        <property type="entry name" value="RasGAP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN WITH IQ MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149:SF10">
        <property type="entry name" value="RAS GTPASE-ACTIVATING-LIKE PROTEIN IQGAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03836">
        <property type="entry name" value="RasGAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GAP" evidence="1">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="498" mass="56713" checksum="1FDF1C208D4145FE" modified="2018-12-05" version="1">MLVNFYRQAHGQNALRDSLGPALRDVLLDQTFSIRTDPLEVYKTWINQTETQTGHKSSLPYEVSAEEALSHPEVQRRIDIAIINLKNLTDRVLKAITSNLNKLPYGLRYTAKVLRDALKTKFPAASEAELYKIVGNLVYYRYMNPAIVAPDGFDVVDRSAGSALQPEQRHILGSIARMLQHAAANKHFHGDGYHVHALNQYITQTHGRFRKFLHSVCDVPEPEERFSMDEFSEQLIVNRPIVYISVSELLNTHKLLLEHQEVLCPDPSDPLRLILNDLGPVPSLQESARCSALLTCGSGFSTKQLIIDVIRTQPGDSLRDILKTKTSPDQEVCHNWLVQRRARQDAQTPEKMKRNPSLVANGNLSLEEKKRKIVRSLRRLEALGVLRPPRAEEQLLQMVAKDIRQQRVRRQRRGAELHKLQQTLSRLQEKSSFHCQQVEFYRHYITSCLDYLTTGSKGTKRNAAESRGKKKLPALSYSAARLQEKGVLLEIEDLPTTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MTZ3</accession>
    <name>A0A3B5MTZ3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endoplasmic reticulum transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BCAP29/BCAP31 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MTZ3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027879"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028215.1">
        <property type="protein sequence ID" value="ENSXCOP00000027879.1"/>
        <property type="gene ID" value="ENSXCOG00000020793.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011863"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008417">
        <property type="entry name" value="BAP29/BAP31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040463">
        <property type="entry name" value="BAP29/BAP31_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701:SF5">
        <property type="entry name" value="B-CELL RECEPTOR-ASSOCIATED PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701">
        <property type="entry name" value="BCR-ASSOCIATED PROTEIN, BAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05529">
        <property type="entry name" value="Bap31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="BAP29/BAP31 transmembrane" evidence="6">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="181" mass="21314" checksum="3115EEF954F232A0" modified="2018-12-05" version="1">MTLQWTAVAFFLYAEIVLNLILCIPFISAHRWHLVFHWRIWSWLSPYWNKFFFAMIMALVVLFCDAIREVQKYSGPEPMHDAQANPNLYDHVHMKLFRAQRNLYICGFSLFLWLVMRRIVTLQNQIAETSVNTAGLQAQMDNAFKTAEKHQEDNQILKTVRISRGCIQDFQEVSSMLAQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MU77</accession>
    <name>A0A3B5MU77_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cornichon family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MU77"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023034"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023311.1">
        <property type="protein sequence ID" value="ENSXCOP00000023034.1"/>
        <property type="gene ID" value="ENSXCOG00000017218.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182834"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003377">
        <property type="entry name" value="Cornichon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12290">
        <property type="entry name" value="CORNICHON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03311">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01398">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="153" mass="17746" checksum="D5832D11290B3C36" modified="2018-12-05" version="1">MEATVFILSLVDCCALIFLSVYFIITLSDLECDYINARACCSKLNKVIICPEICLCYCFQWVIPEMVGQCLSMMLMLVSMHWFIFLLNLPVAAWNIYRYVKIPMGNMGVFDPTEIHNRGLLKSYMKEAMIKLGYHLLCFFIYLYSMILALIND</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUK3</accession>
    <name>A0A3B5MUK3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Insulin-like growth factor binding protein 6a</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MUK3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024927"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025231.1">
        <property type="protein sequence ID" value="ENSXCOP00000024927.1"/>
        <property type="gene ID" value="ENSXCOG00000018612.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160528"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001968">
        <property type="term" value="F:fibronectin binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="Thyroglobulin type-1"/>
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        <property type="entry name" value="IGFBP-like"/>
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    <dbReference type="PROSITE" id="PS51323">
        <property type="entry name" value="IGFBP_N_2"/>
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        <property type="entry name" value="THYROGLOBULIN_1_1"/>
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    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017216644" evidence="6">
        <location>
            <begin position="19"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="IGFBP N-terminal" evidence="8">
        <location>
            <begin position="43"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Thyroglobulin type-1" evidence="7">
        <location>
            <begin position="102"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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            <dbReference type="ARBA" id="ARBA00023157"/>
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            <dbReference type="ARBA" id="ARBA00023183"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00500"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
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            <dbReference type="PROSITE" id="PS51323"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    <sequence length="176" mass="19138" checksum="539C1854E46B5661" modified="2018-12-05" version="1">MLLYVNILILLVRDVALSSILTTPSRGCPTCTGKPVGLNVASLAPGEPCGVYTLNCAPGLRCEPPQDEPRPLRALLEGRGVCSNVSSTTPTPQVYPAGKAYRAPCRKLLMKVIQGLDAHLFKSLHDIYIPNCDKSGFYKRKQCWSSLGKQRGKCWCVDENGLPVPPKTKQQGNVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUR3</accession>
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            <fullName evidence="10">EF-hand domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Xiphophorus couchianus</name>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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            <dbReference type="ARBA" id="ARBA00004308"/>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="92" mass="9616" checksum="1FBEC32EF6623D9E" modified="2018-12-05" version="1">MGGHMGGPMGGPGPQPGYGSYGGAGYTGGYGAPTPAANDPMWGYFTAIAGQDGEVDAEELQRCLTQAGFTGSYNRKFIYASLSWLKPDTYKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZE2</accession>
    <name>A0A3B5MZE2_9TELE</name>
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        <recommendedName>
            <fullName evidence="4">Polyhomeotic homolog 1</fullName>
        </recommendedName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Atherinomorphae</taxon>
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            <taxon>Poeciliidae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025915.1"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <sequence length="179" mass="19219" checksum="7141B0876C53F8DF" modified="2018-12-05" version="1">MESGEDQNTSSTNGNPQTSGNSRAPQIARMSLYERQAVQALQALQRQPNAAQYFQQLMLQQQINTAQLQNFAVQQATLAASRQSNTPSNSISPAPTTVNLSTTSAGGTMSSPRPHGPATSATTTALNQSVLLGGNSAGQGQMYLRVSLKCTHMLPITRFMCSMCRTWLYTVPLTPKPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZN4</accession>
    <name>A0A3B5MZN4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Caspase a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Atherinomorphae</taxon>
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            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peptidase C14A family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A3B5MZN4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027697"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028032.1">
        <property type="protein sequence ID" value="ENSXCOP00000027697.1"/>
        <property type="gene ID" value="ENSXCOG00000020650.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:AIM2 inflammasome complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:NLRP3 inflammasome complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:cysteine-type endopeptidase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:regulation of inflammatory response"/>
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    </dbReference>
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        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Caspase_cys_AS"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
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        <property type="entry name" value="Pept_C14_p10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47901">
        <property type="entry name" value="CASPASE RECRUITMENT DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47901:SF3">
        <property type="entry name" value="CASPASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00376">
        <property type="entry name" value="IL1BCENZYME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01122">
        <property type="entry name" value="CASPASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50207">
        <property type="entry name" value="CASPASE_P10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p20" evidence="6">
        <location>
            <begin position="71"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p10" evidence="5">
        <location>
            <begin position="227"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="35419" checksum="7231A4BC4912EE0B" modified="2018-12-05" version="1">TSQRTRKILRQDEGKFRAARQEAEERYTQEEVTLRSKTCCSKSVLFLPAPSPFLFSLFICQVYPVTEDNYRNRVALMITNIKFSDEKLNRDGAEKDEENMEKLLKSLGYEVVKHRNLTGKGIDDALKSFAKLDKLKRTDSVFVVIMSHGKLGKILGIDYKEDNQDDFAVDKIFKCLNSHGCQALINKPKVVILQACRGGRSGVTTWVHDGESTGPPAPPPEDQEPNLDQSVHVEKDFVSLLASTPDNKAFRESKNGSILIQNIMELIVHAVSTPQVQDLVSKCGDDKKKQMPVIDRMTLNKQFFLYPGLLDSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZQ2</accession>
    <name>A0A3B5MZQ2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MZQ2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026110"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026424.1">
        <property type="protein sequence ID" value="ENSXCOP00000026110.1"/>
        <property type="gene ID" value="ENSXCOG00000019501.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157585"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18947">
        <property type="entry name" value="bHLH-PAS_ARNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000003">
        <property type="entry name" value="Aryl hydrocarbon receptor nuclear translocator 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000011">
        <property type="entry name" value="Aryl hydrocarbon receptor nuclear translocator 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050933">
        <property type="entry name" value="Circadian_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001067">
        <property type="entry name" value="Nuc_translocat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23042">
        <property type="entry name" value="CIRCADIAN PROTEIN CLOCK/ARNT/BMAL/PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14598">
        <property type="entry name" value="PAS_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00785">
        <property type="entry name" value="NCTRNSLOCATR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="10">
        <location>
            <begin position="5"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="77"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="9">
        <location>
            <begin position="286"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="430"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="439"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="497"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="564"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="577"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="609"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="664" mass="73421" checksum="E6ECBE9BBD30E540" modified="2018-12-05" version="1">GSSNEKEENHSEIERRRRNKMSAYIGELSDMVPSCSALARKPDKLTILRMAVSHMKSLRGNANTNADGSYKPSFLSDQELKHLILEAADGFLFVVSCETGRVVYVSDSVTPVLNQLQSEWLGSSLYDQLHPDDTEKLREQLSTSENNNTGRMLDLKTGTVKKESQQSSARMTMGARRSFICRMRCGTCPVEPMSMNRLSFFRSRNRNGLGTPKEGEPQYVVVHCTGYVKSWPPAGVSLTDDEADNNQGSRYCLVAIGRLQVTCCPGDTDLNSISVPVEFISRHNCQGMFTFVDHRCLTSIGYQPQELLGKNILDFSHPEDQGLLRDSFDQVVKLKGQVLSVMFRFRAKTRDWMLMRTSSFTFQNPFSEEIEYIICTNVNLQQQQQQADLEGGATRDGMYEAGPINIQQQMPVQPVVAVGPDHSKTIEKQEIYPPIFPGSDQTKGMPTNSTPSTQIYTNNFSAGRSNDSYRPVAMPPQMTQPAHSAPQMLPHMPRQNGAPQSVTPSSTNSPLHGPSGGWPAPGPAGRPQFSNQQVAPQAAKTMSQQFPPIGGFGGGSSNTFSQIPTGAAPTPTNGTNYPQMNPRTSLNTNGYDTSQSGGQFPPRVTEGVWPQWPGQQQHPQNNREQHPHAQGNQQDIFPDVLSMLDQPAAFNSDDFEIAIYPPYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N0N1</accession>
    <name>A0A3B5N0N1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">WD repeat domain 33</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5N0N1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026765"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027094.1">
        <property type="protein sequence ID" value="ENSXCOP00000026765.1"/>
        <property type="gene ID" value="ENSXCOG00000019993.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111130"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000778">
        <property type="entry name" value="WD repeat domain 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045245">
        <property type="entry name" value="Pfs2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836:SF0">
        <property type="entry name" value="PRE-MRNA 3' END PROCESSING PROTEIN WDR33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="157"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="293" mass="33730" checksum="8D12239A9D666888" modified="2018-12-05" version="1">MATDVGSPQRFFHMPRFQHQAPRQVFYKRPDFAQQQAMQQLTFDGKRMRKAVNRKTIDYNPSVIRYLENRLWQRDHRDFRAIQPDAGCYNDLVPPLGMLNNPMNAVTTKFVRTSTNKVKCPVFVIRWTPEGRRLVTGASSGEFTLWNGLTFNFETILQAHDSPVRAMTWSHNDMWMLTADHSGYVKYWQSNMNNVKMFQAHKEAIREARFAPSLSAAPMFWFGSDAAAIFSPGTLWVTSCVLVPTTTPGIHPYWYRYMTRTEPGRNSTCCFGLTLIWFCSTVSSGPGTDPETR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KPC0</accession>
    <name>A0A3B5KPC0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zona pellucida sperm-binding protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Zona pellucida</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="2">The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.</text>
    </comment>
    <comment type="PTM">
        <text evidence="2">Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZP domain family. ZPC subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KPC0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001968"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001996.1">
        <property type="protein sequence ID" value="ENSXCOP00000001968.1"/>
        <property type="gene ID" value="ENSXCOG00000001566.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234567"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035805">
        <property type="term" value="C:egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032190">
        <property type="term" value="F:acrosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035804">
        <property type="term" value="F:structural constituent of egg coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035803">
        <property type="term" value="P:egg coat formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000344">
        <property type="term" value="P:positive regulation of acrosome reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.4100:FF:000002">
        <property type="entry name" value="Zona pellucida sperm-binding protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4100">
        <property type="entry name" value="Zona pellucida, ZP-C domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3210">
        <property type="entry name" value="Zona pellucida, ZP-N domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055355">
        <property type="entry name" value="ZP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042235">
        <property type="entry name" value="ZP-C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055356">
        <property type="entry name" value="ZP-N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001507">
        <property type="entry name" value="ZP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11576:SF2">
        <property type="entry name" value="ZONA PELLUCIDA SPERM-BINDING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00100">
        <property type="entry name" value="Zona_pellucida"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23344">
        <property type="entry name" value="ZP-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00241">
        <property type="entry name" value="ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51034">
        <property type="entry name" value="ZP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="1 2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5041482950" description="Zona pellucida sperm-binding protein 3" evidence="2">
        <location>
            <begin position="21"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="ZP" evidence="4">
        <location>
            <begin position="45"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="358" mass="39213" checksum="B267C5BE3BBE6386" modified="2018-12-05" version="1">AMCVRLSMWFIVLCLTLTAGLQSDHDDAEESGQQRSELSGGQHGDCDETPPVGPQAQLVGLRLGPSGSGRDRCAASRSADGEFIIRAPLAECGSRFTDDAVLYSNMLLFFPGSSAGDTFKTEGAAVPVLCRYKSSGALRPTWTSVVSVHSAHLSLDFYLSLMTDDWSSERNPPVYFMSEMVNILASIDHHHHHHPPLRLYAGSCVATLTPDVDSHPRYPFIDHLGCFTDSQLSGSGSRFLPRVRDDLLHIQLEPFLFHQDWRHSVSIYITCYLEAVPLSKKDPEKKACSFITGRWRSADGDDHACESCDRPGEAAEGSSAYFIHERAQRSNRGRGLNGVMCAGFPPGSQCAGHVNSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KPL7</accession>
    <name>A0A3B5KPL7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Integrin beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Cell membrane</location>
            <topology evidence="1 17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the integrin beta chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KPL7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002078"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002108.1">
        <property type="protein sequence ID" value="ENSXCOP00000002078.1"/>
        <property type="gene ID" value="ENSXCOG00000001671.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286919"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034685">
        <property type="term" value="C:integrin alphav-beta6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000002">
        <property type="entry name" value="Integrin beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033760">
        <property type="entry name" value="Integrin_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015812">
        <property type="entry name" value="Integrin_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002369">
        <property type="entry name" value="Integrin_bsu_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082">
        <property type="entry name" value="INTEGRIN BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10082:SF11">
        <property type="entry name" value="INTEGRIN BETA-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00362">
        <property type="entry name" value="Integrin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17205">
        <property type="entry name" value="PSI_integrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01186">
        <property type="entry name" value="INTEGRINB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00187">
        <property type="entry name" value="INB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 17" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13 17" id="KW-0401">Integrin</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="PSI" evidence="19">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin beta subunit VWA" evidence="18">
        <location>
            <begin position="9"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="195" mass="21774" checksum="B326B3A813C231CB" modified="2018-12-05" version="1">SCSTVSAVTCDECLQLSPHCGWCTQENFTDWVSISSRCDTLDSLLEKGCSRDQVQFPVSESQILLDRPLGKITDDANSTQISPQKMSLKLRPSVETFQVKVQPTADYPVDMYYMMDLSASLIDDLKMIKDLGSSLSREMSKLTSNFQMGFGSFVEKPVLPYIKITEKELANPCDFGISCHPTFGYKHVLSLTSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQH8</accession>
    <name>A0A3B5KQH8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">creatine kinase</fullName>
            <ecNumber evidence="2">2.7.3.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>creatine + ATP = N-phosphocreatine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17157"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57947"/>
            <dbReference type="ChEBI" id="CHEBI:58092"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.3.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:17158"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 10">Belongs to the ATP:guanido phosphotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.3.2" evidence="2"/>
    <dbReference type="RefSeq" id="XP_027896456.1">
        <property type="nucleotide sequence ID" value="XM_028040655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQH8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000130"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000131.1">
        <property type="protein sequence ID" value="ENSXCOP00000000130.1"/>
        <property type="gene ID" value="ENSXCOG00000000121.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114158790"/>
    <dbReference type="KEGG" id="xco:114158790"/>
    <dbReference type="GeneTree" id="ENSGT00950000182772"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004111">
        <property type="term" value="F:creatine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046314">
        <property type="term" value="P:phosphocreatine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00716">
        <property type="entry name" value="creatine_kinase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000026">
        <property type="entry name" value="Creatine kinase B-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.135.10:FF:000001">
        <property type="entry name" value="Creatine kinase M-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.135.10">
        <property type="entry name" value="ATP:guanido phosphotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000749">
        <property type="entry name" value="ATP-guanido_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022415">
        <property type="entry name" value="ATP-guanido_PTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022414">
        <property type="entry name" value="ATP-guanido_PTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022413">
        <property type="entry name" value="ATP-guanido_PTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036802">
        <property type="entry name" value="ATP-guanido_PTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547">
        <property type="entry name" value="ARGININE OR CREATINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547:SF23">
        <property type="entry name" value="CREATINE KINASE B-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00217">
        <property type="entry name" value="ATP-gua_Ptrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02807">
        <property type="entry name" value="ATP-gua_PtransN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48034">
        <property type="entry name" value="Guanido kinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00112">
        <property type="entry name" value="PHOSPHAGEN_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51510">
        <property type="entry name" value="PHOSPHAGEN_KINASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51509">
        <property type="entry name" value="PHOSPHAGEN_KINASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphagen kinase N-terminal" evidence="12">
        <location>
            <begin position="11"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphagen kinase C-terminal" evidence="13">
        <location>
            <begin position="125"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="96"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="128"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="292"/>
            <end position="296"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="320"/>
            <end position="325"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="381" mass="42994" checksum="B897233A2B553F46" modified="2018-12-05" version="1">MPFGNTHNKFKMNYSAEQEFPDLSQHNNHMAKVLTLDMYAQLRDKETPSGFTLDDLIQTGVDNPGHPFIMTVGCVAGDEETYDVFKDLMDPVIEDRHGGYKPSDKHKTDLNAENLEGGDDLDPNYVLSSRVRTGRSIRGFCLPPHCSRGERRAIEQLSIEALDSLEGDLKGKYYALKNMTDEEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKTFLVWVNEEDHLRVISMQKGGNMREVFTRFCTGLTKIEDLFKNRGHEFMWNEHLGYVLTCPSNLGTGLRAGVHVKLPNVSKHEKFGEILKRLRLQKRGTGGVDTAAVGGVFDISNADRLGFSEVELVQMVVDGVNLLVNMEKRLEAGEAINDLMPEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRC4</accession>
    <name>A0A3B5KRC4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mitochondrial intermediate peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MIPEP</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRC4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000191"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000196.1">
        <property type="protein sequence ID" value="ENSXCOP00000000191.1"/>
        <property type="gene ID" value="ENSXCOG00000000164.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183171"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06457">
        <property type="entry name" value="M3A_MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000013">
        <property type="entry name" value="Mitochondrial intermediate peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033851">
        <property type="entry name" value="M3A_MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF71">
        <property type="entry name" value="MITOCHONDRIAL INTERMEDIATE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="7">
        <location>
            <begin position="182"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="597" mass="68244" checksum="3E5F9A4ED5C637FB" modified="2018-12-05" version="1">MSASQRLLHLVKYRSLWTHVRRKVTTWSPVGAAFNAKPFRSLDLVEKNADFIKVAHPDPAYREAAEKTCIDIGTVVEKLNTNVELCKSLKNLLDNAKVVAELDPDTKRVAELFMFDFEISGIHLDDKLRKEAVALHVKLLDLSNEFLIGSHVPNRIAKSAIPKHLQVLFASDGSFIQIGGLHADSPDDLLREIAYRIYLYPNADLMECLEELLKCRYKLAKLVGYDSYGHRALKGTMAKNPETVMSFLQLLTDKLSQRTAKDFQMMRDMKKKLNPRNAELMPWDHPFLSGVLRAERYNIEPSLYSPYLSLGACMEGLNNLFSQLYGVSLMSEHPGAGEVWSEDVRKLAVVHETEGLLGYIYCDFFHRRDKPHQDCHFTIRGGRWCQETDQYQLPVVVLMLSLPHPTKSAPTLLTPGMMENLFHEMGHAMHSMLGRTRYQHVTGTRCATDFAEVPSILMEYFATDYRVISQFARHFETGQPLPENMVARLCESKKVCGAADTQLQVFYAVLDQIYHGKPQNRSTTEILKEMQEKFYGLPYTPNTAWQLRFSHLIGYGAKYYSYLMSRAVASMVWKQCFVQDPLNRLFIDAESTFGKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRD9</accession>
    <name>A0A3B5KRD9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRD9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000206"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000212.1">
        <property type="protein sequence ID" value="ENSXCOP00000000206.1"/>
        <property type="gene ID" value="ENSXCOG00000000202.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111690"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38706:SF2">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38706">
        <property type="entry name" value="SI:CH211-198C19.1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="246" mass="28754" checksum="D478935C8606D9BD" modified="2018-12-05" version="1">MKIQFKLQKRKTKKQQLYFSNTFLELLSSSGFVWFRYSRCWLKLLCWFANTVSIDNRGNVRLTFEPESDYGSHHYGNFEGMLNPPPFGYKYYTVGNIPTSASLPSYVVNSQRNTVGNSARILIRVNNGRQIDQVFITQHYDEYENQGSSYDPEQTFRITTNFLRQLRKHSVNEIQQLADGSQTGPIRNTSNAYSHEIHQPKQDMQIDMPYTPVRQDDNCIANVYCLICALFIILFIFGSYFGNWRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KSE0</accession>
    <name>A0A3B5KSE0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NHL repeat-containing protein 3</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027882645.1">
        <property type="nucleotide sequence ID" value="XM_028026844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KSE0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000546"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000555.1">
        <property type="protein sequence ID" value="ENSXCOP00000000546.1"/>
        <property type="gene ID" value="ENSXCOG00000000483.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114150445"/>
    <dbReference type="KEGG" id="xco:114150445"/>
    <dbReference type="GeneTree" id="ENSGT00390000008657"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000043">
        <property type="entry name" value="NHL repeat containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10680">
        <property type="entry name" value="PEPTIDYL-GLYCINE ALPHA-AMIDATING MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63829">
        <property type="entry name" value="Calcium-dependent phosphotriesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51125">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="5">
        <location>
            <begin position="215"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00071346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="355" mass="38930" checksum="38D79D67CA2A63D2" modified="2018-12-05" version="1">MLIKKRSHWCLIASGMVSLILFVTLLYNGSSGGGLRRSSSSLRHDYQLMGRPLYKLDLGWPNNPEIFTGEVFCVAVNQYAAVVYVAQRGANVPKVLVFTTEGEFLMSWNTTTLEMPHGMFLADASSKDPTIWITDVGDGPYGHSIKQYSQSGKLLQVLGTPGKAGSGLDPLQFDQPAEIFVHESGEMYIVDGDGGMNNRLIKLSKDQKVLWMHAEKGQGLAQFYIPHSVTVDSAQRVWVADRGNKRIQVFNSITGDWLGTWGSCFTEDAPYSVRLTPDKKYFVVVQLNTNQITLLDAPPVGLIGQCKVASVIQLAEDVKPHLVDLDLKAGALYVAEIGAQQAQKFTPFSLGGSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KWK8</accession>
    <name>A0A3B5KWK8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KWK8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002041.1">
        <property type="protein sequence ID" value="ENSXCOP00000002011.1"/>
        <property type="gene ID" value="ENSXCOG00000001620.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166133"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052477">
        <property type="entry name" value="Orphan_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46272:SF4">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46272">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="136"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="30"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="343" mass="39145" checksum="81B7C110616E0955" modified="2018-12-05" version="1">SPHTHFPFPQMTHIPPGKQHGFLVLCFLPANLFTFYMICFRKSGMSNTAIVYLSCLAIVDTFYVVWVILLDLTLTFWTVQPFWHSNPWCGILGFLQYGSLYSSSWIVVVFTIERYLVLRSTASKQNFSQTWDTKMFCTAIVLLSHLVSVPMGWINIVTPVNLTVHGENMTLPRCRYRNHTYSTVIVWIATFLSGGIPIVLERRIMYGRNTRGTLTRTIFILGTVSVAFVVLSLPRFVTYCILRTRYNDESFNRNDYSIPINVAGDVANMLQNLNSTTNFLLYCMVSHRFRQELVQAMTCKAKARELSTLLNHTTMKVFSVVNHKASLSSSPVTVVLTSLKQTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KWQ0</accession>
    <name>A0A3B5KWQ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Syntaxin-1B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Epimorphin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Syntaxin-2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Essential for epithelial morphogenesis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Potentially involved in docking of synaptic vesicles at presynaptic active zones. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with OTOF. Interacts with SYT6 and SYT8; the interaction is Ca(2+)-dependent.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KWQ0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002056"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002086.1">
        <property type="protein sequence ID" value="ENSXCOP00000002056.1"/>
        <property type="gene ID" value="ENSXCOG00000001655.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234627"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048787">
        <property type="term" value="C:presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098967">
        <property type="term" value="P:exocytic insertion of neurotransmitter receptor to postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031629">
        <property type="term" value="P:synaptic vesicle fusion to presynaptic active zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15880">
        <property type="entry name" value="SNARE_syntaxin1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00179">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000005">
        <property type="entry name" value="Syntaxin 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.70:FF:000001">
        <property type="entry name" value="Syntaxin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF334">
        <property type="entry name" value="SYNTAXIN-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00804">
        <property type="entry name" value="Syntaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00503">
        <property type="entry name" value="SynN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="19">
        <location>
            <begin position="167"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="12"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="264" mass="30341" checksum="EFD417C227D47F1F" modified="2018-12-05" version="1">NTFKTEEIDSYVEEIRGCIEKLSEDVEQVKKQHSAILAAPNPDEKTKQELEDLTADIKKTANKVRSKLKAIEQSIEQEEGLNRSSADLRIRKTQHSTLSRKFVEVMTEYNTTQSKYRDRCKDRIQRQLEITGRTTTNEELEDMLESGKLAIFTDDIKMDSQMTKQALNEIETRHTEIIKLENSIRELHDMFVDMAMLVESQGEMIDRIEYNVEHSVDYVERAVSDTKKAVKYQSQARKKKIMIIICCVILGVVLASTIGGTLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXD4</accession>
    <name>A0A3B5KXD4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXD4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000224"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000230.1">
        <property type="protein sequence ID" value="ENSXCOP00000000224.1"/>
        <property type="gene ID" value="ENSXCOG00000000212.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00930000151044"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16515">
        <property type="entry name" value="RING-HC_LRSAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000002">
        <property type="entry name" value="Baculoviral IAP repeat containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF47">
        <property type="entry name" value="LEUCINE RICH REPEAT AND STERILE ALPHA MOTIF CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="478"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="402"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="523" mass="59821" checksum="D16381DB8938F36E" modified="2018-12-05" version="1">MPLFSRRRKPSEDSKKRLEHQLYWSNQEGADDILDVSACELTELQLPTKVFSICKVQQKKVLILHTNELKFLLPKGCDICCLATLKVLDLHENKLSVLPEEIGTLTKLQILNVEKNRLKVLPESVCRLQLLQTLNLKNCLMELPAAIGSLSSLRTLDISDNNVLQLPKHLAHIRTLELGEEYCAPSQYLLPVLESECSKPGSDCLDGLEEAWQSKFSDYERRKELKLQEKLAFERHLEEKHREHTQLLLMNNSHKENILNSDQQRVELGFSQQQRAQEAERQQVLERVRQAEDNISGRISSLLMDNSQKKSAEFLQAMEEDIRMEQLTAVTQEEAGSLRRREVALESLDRKFDRMLSLQVLDKSKAIAQILQEEEMQKAAFQALQLQKDAVHVYIRSQIKLIEAELMQLTKLEVKRRSLDAESLQEVLVEQRTALSDLLQQLLKQRDQREQELRQVLPGTPVTPSAPTPVEGPGSSECVVCMETGVVFLPCGHVCCCQVCSDALQNCPLCRSNISQRIRLYQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXP9</accession>
    <name>A0A3B5KXP9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="38">G-protein coupled estrogen receptor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="39">G protein-coupled estrogen receptor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="40">G-protein coupled receptor 30</fullName>
        </alternativeName>
    </protein>
    <organism evidence="44 45">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="44" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="44" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell projection</location>
            <location evidence="10">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Dendritic spine membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasmic vesicle membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Golgi apparatus membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="37">Postsynaptic density</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="41">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXP9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003332"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003370.1">
        <property type="protein sequence ID" value="ENSXCOP00000003332.1"/>
        <property type="gene ID" value="ENSXCOG00000002634.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154307"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032591">
        <property type="term" value="C:dendritic spine membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:nuclear estrogen receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:apoptotic process"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0071392">
        <property type="term" value="P:cellular response to estradiol stimulus"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047143">
        <property type="entry name" value="GPER1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24226:SF2">
        <property type="entry name" value="G-PROTEIN COUPLED ESTROGEN RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24226">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 182 AND ESTROGEN RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="17" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="35" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="34" id="KW-0966">Cell projection</keyword>
    <keyword evidence="15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="36" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="30" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="19" id="KW-0221">Differentiation</keyword>
    <keyword evidence="28" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="20" id="KW-0256">Endoplasmic reticulum</keyword>
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    <keyword evidence="25 41" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="24" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="27 42" id="KW-0472">Membrane</keyword>
    <keyword evidence="26" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="21" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="32" id="KW-0539">Nucleus</keyword>
    <keyword evidence="33" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="29 41" id="KW-0675">Receptor</keyword>
    <keyword evidence="45" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="23" id="KW-0770">Synapse</keyword>
    <keyword evidence="31 41" id="KW-0807">Transducer</keyword>
    <keyword evidence="16 41 42" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="22 42" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="50"/>
            <end position="71"/>
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    <feature type="transmembrane region" description="Helical" evidence="42">
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    <feature type="transmembrane region" description="Helical" evidence="42">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="42">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="42">
        <location>
            <begin position="296"/>
            <end position="315"/>
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    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="43">
        <location>
            <begin position="62"/>
            <end position="312"/>
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        </source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="45">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="377" mass="42086" checksum="80D3863F684E7B08" modified="2018-12-05" version="1">RKTGAMKPDMDINMFTDPRAGENLTVPPNLPQQASENIKHYLINLFLSGFYTLILFPVGLVGNFLILVVNLAHQGRLSAPDLYFANLAAADLILVADSLIEVFNLKKGYYDNAGLCTFMNLFQQVNLYSSVFFLTWMSIDRFVALTRIAAGACTAHACLSCCLIWAFAVMLTLLPFAIAQVQHYGELNFCFANVSQVQWLEVTLGFLLPLCILAVCYWRIAQVLRHSQRHRCYLQQRPRRQQALRMIAAAVLVFFVCWLPVNVFISVHLLSGADAAGANTAGANTLWELYPLTGHAVRLAAFSNSCLNPLIYGFLGETFRKKLRLFLQEKSRSAKLQRFASEKHIYAPVAPTCQHKTCDTWSPSTKSSSNGGKSLVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYA1</accession>
    <name>A0A3B5KYA1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Interleukin-1 beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
            <location evidence="3">Extracellular exosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the IL-1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KYA1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000529"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000538.1">
        <property type="protein sequence ID" value="ENSXCOP00000000529.1"/>
        <property type="gene ID" value="ENSXCOG00000000441.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016316"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019221">
        <property type="term" value="P:cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001660">
        <property type="term" value="P:fever generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048246">
        <property type="term" value="P:macrophage chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042119">
        <property type="term" value="P:neutrophil activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901222">
        <property type="term" value="P:regulation of non-canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00100">
        <property type="entry name" value="beta-trefoil_IL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000975">
        <property type="entry name" value="IL-1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10078:SF30">
        <property type="entry name" value="INTERLEUKIN-1 BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10078">
        <property type="entry name" value="INTERLEUKIN-1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00340">
        <property type="entry name" value="IL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00125">
        <property type="entry name" value="IL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0202">Cytokine</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="11" id="KW-0458">Lysosome</keyword>
    <keyword evidence="12" id="KW-0497">Mitogen</keyword>
    <keyword evidence="9" id="KW-0666">Pyrogen</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="360" mass="41023" checksum="3AF392E116165834" modified="2018-12-05" version="1">MLYLFTLVTELKDLKIVFAHVLQGSTVKGSVLIRHQLHEGKHHYEVEEVVKYQTKTDRFIRGDKLTEINGVDLRDLEPEELAKMIAEGNPLLVCQLSEKEEQLPLDEDTLQPYDKESTVLSFSWELTREEGANEGEKNETTDLKGNVCQDMSKENGESRELLVIHMTNTSIAVVGTRGCEECEGTGCTVSDIVVVAESSAVTFDVSFRQEKLADVLIKHVPTHRYLRTICSEKTVYSSPNPEKITIYYYKSNAMDRTFRGIAVVLNLTGSNCFLRCCKEGDRVLLQVETCEKQRLRQISKNDDCTFSFVFYMMADRTKQRKFESALHNGWFIHVANTDLVEMAETDGERGDSSFLFIIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYE5</accession>
    <name>A0A3B5KYE5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cation efflux protein transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KYE5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003050"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003087.1">
        <property type="protein sequence ID" value="ENSXCOP00000003050.1"/>
        <property type="gene ID" value="ENSXCOG00000002419.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156484"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019855">
        <property type="term" value="F:calcium channel inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010312">
        <property type="term" value="P:detoxification of zinc ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820">
        <property type="entry name" value="FI23527P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820:SF1">
        <property type="entry name" value="PROTON-COUPLED ZINC ANTIPORTER SLC30A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="8">
        <location>
            <begin position="19"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="106" mass="11679" checksum="7E1F4B89F2145EEB" modified="2018-12-05" version="1">MGIQQRSKMACGPNRVRLLCMLALTFGFFVVEVVVSRITSSLSMLSDSFHMLSDVLALVVALVAVRFAERTHSTNKNTFGWIRAEVMGALVNAIFLTASTSRFFST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYH2</accession>
    <name>A0A3B5KYH2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribitol-5-phosphate transferase FKTN N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KYH2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000599"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000609.1">
        <property type="protein sequence ID" value="ENSXCOP00000000599.1"/>
        <property type="gene ID" value="ENSXCOG00000000515.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014471"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045587">
        <property type="entry name" value="FKTN_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19737">
        <property type="entry name" value="FKTN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Ribitol-5-phosphate transferase FKTN N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="443" mass="50808" checksum="2C43B2252B4924F2" modified="2018-12-05" version="1">MPRVNRTFVFVLLLASSCAFLLFQLYYYRNYVSKVRGGTPRSGKMFLNLKKFLALAQFFRLPLFLADSAALALLSQDALRQRDRVRREPHCSFLCTGRPITSFALPPGFLLAAEQKGFQLLEVRGRDPRLASLDTLSGEEIPLHFLFRLHGYIIHVVLLYERSGGYLWHGVLRLKAAADPTFAPFRLLDYGRHAGAYDRPELLLTVVDGLDVRVPRNISRFLVQQQEARFLECRYHDARSFLQLYPDDSSTAAVEFRRKAKALLRLAAQTLDRLDVPFWISSGTCLGWFRQCGVISYSRDVDIGIFISDFRPQITAAFRNVGMSLKHQFGKVEDSLELSFLGEDVKLDIFFFYRDGDVVWNGGTQAKSGRKFKYIFPSFSLCWAELLEVKVRVPCETLDYVMANYGTTWNVPVKSWDWKSSPSNVIENGVWPVAEWARLIQVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZX9</accession>
    <name>A0A3B5KZX9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Calcineurin B homologous protein 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Tescalcin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Ruffle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the calcineurin regulatory subunit family. CHP subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZX9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004107"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004151.1">
        <property type="protein sequence ID" value="ENSXCOP00000004107.1"/>
        <property type="gene ID" value="ENSXCOG00000003224.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155845"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052490">
        <property type="entry name" value="CHP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46823">
        <property type="entry name" value="CALCINEURIN B HOMOLOGOUS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="19"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="125" mass="14479" checksum="C70F5D1AF8A7F6AF" modified="2018-12-05" version="1">MSHFRPPSPKITDEQRKNIRREKLQFIFNMHDSDGDGLVTLEDYRHVVEELLSRSGALGKETARAIADAAMFEVARTVGHKDPNEFYEGITFEHFLKLLSNFDIESRMNIRFLKSDVNPFCQLCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L061</accession>
    <name>A0A3B5L061_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L061"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003735.1">
        <property type="protein sequence ID" value="ENSXCOP00000003695.1"/>
        <property type="gene ID" value="ENSXCOG00000002899.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164173"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF287">
        <property type="entry name" value="CADHERIN-RELATED NEURONAL RECEPTOR VARIABLE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="25"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="13">
        <location>
            <begin position="132"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="283" mass="30992" checksum="66D36B54FE92080C" modified="2018-12-05" version="1">MNSVQEIPVLPFTIFIILGCGWISVSGQLSYSISEEVNPGTSLGNIAKDLNLGLHELEARMFQIVNGAKKKHFGVNLKTGVLFVNDRIDREELCAKAKKCTVSVEAVINSPLKLYRLEINIIDVNDNFPSFTSTSQNIEIAESTLPGTKFLVLSAYDADVGQNDISAYQLSTNDHFSLSVNKGEKVSAELILQKPLDREKQSVISLTLTAVDGGTPVKSGTSQLVIHVLDINDNVPMFAQPLYKSKSVRQRKAVCDSAPTDGFGRRNACKIRLHGGYRKCVGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L0D1</accession>
    <name>A0A3B5L0D1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SH3 domain-binding protein 5</fullName>
            <shortName evidence="3">SH3BP-5</shortName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Functions as guanine nucleotide exchange factor (GEF) for RAB11A.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with GDP-bound and nucleotide-free forms of RAB11A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="3">Colocalizes with RAB11A on cytoplasmic vesicle membranes.</text>
    </comment>
    <comment type="domain">
        <text evidence="3">The N-terminal half of the protein mediates interaction with RAB11A and functions as guanine nucleotide exchange factor. Four long alpha-helices (interrupted by a central kink) assemble into coiled coils, giving rise to a 'V' shape.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SH3BP5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L0D1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005788"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005854.1">
        <property type="protein sequence ID" value="ENSXCOP00000005788.1"/>
        <property type="gene ID" value="ENSXCOG00000004493.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018500"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007940">
        <property type="entry name" value="SH3BP5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19423">
        <property type="entry name" value="SH3 DOMAIN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19423:SF11">
        <property type="entry name" value="SH3 DOMAIN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05276">
        <property type="entry name" value="SH3BP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="170"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="222"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="286"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="188"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="237"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU369054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="351" mass="38803" checksum="8E06E99A93916582" modified="2018-12-05" version="1">KKTSQSTTVLNSNVLNISDFFSFVQKAQLEAQKATQDFHRATEVLRAAKETISLAEQRLLEEDNRQFDSAWQEMLNHATQRVMEAEHTKTRSELLHKETAAKYTAAIGRMKQLEKKLKRTISKSKPYFEMKAKYYLQLENLKKNVDERQAKLSQAKGEYRTALKNLEMISDEIHERRRSSTIGPRRPGVGAEGDSSSGDDVSSFKMESDGISMASECFDDEPCGGSSFMSEEDSETRSTSSLGSTPSSPQELLSPCPFAGSSSSGTLDLPSTVSLSDFGLISPVLGPRSECSGASSPECDLERGDGGEDAPGFTRDSLEGEKSVLSELQQKRSLRAETINHEESIIEIIIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1V3</accession>
    <name>A0A3B5L1V3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1V3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004320"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004367.1">
        <property type="protein sequence ID" value="ENSXCOP00000004320.1"/>
        <property type="gene ID" value="ENSXCOG00000003399.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163071"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061172">
        <property type="term" value="P:regulation of establishment of bipolar cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039269">
        <property type="entry name" value="ANKFN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21437:SF3">
        <property type="entry name" value="ANKYRIN REPEAT AND FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21437">
        <property type="entry name" value="WIDE AWAKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="390" mass="42520" checksum="0D59285E26A1D8A8" modified="2018-12-05" version="1">IGLYCRLSSVLDVDALITQQALREAITDGELTSARSRHQLILDYIQQLDEMRRDLRWITDALQYARYKQPRGGVLITCLVDASASESDSGQQKTDSTSSTMDYMPTPSPSPELRQRKAISDSLLGSDEDGSSEVFLPTDSDYDSSDALSPRELDLLYSPASDLSQQVLHSLGGSAPDVLQIHELRTLSTSPSLPLSPRFYKDLPLSFPLSPALSDTTKTFEGLSLTKEGPAPLRGLANPPAKRKLLSRSHRGQYFSGPQRWLRGHSGESLTGSLSEGVYTRQLDADLTPLGDPASPQGHTYVSHASCVLESRKARHREPRPHVRRIFVEPCKDASPSREEETVTTAGVSVVVAQAESGEEGEEPEGATAQEVDSDDQTNAQVSEILSSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1X5</accession>
    <name>A0A3B5L1X5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1X5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003929.1">
        <property type="protein sequence ID" value="ENSXCOP00000003886.1"/>
        <property type="gene ID" value="ENSXCOG00000003056.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164983"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000018">
        <property type="entry name" value="Protocadherin gamma c3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF236">
        <property type="entry name" value="PROTOCADHERIN GAMMA-C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017421454" description="Cadherin domain-containing protein" evidence="14">
        <location>
            <begin position="27"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="689"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="24"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="131"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="240"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="348"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="453"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="15">
        <location>
            <begin position="578"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="828" mass="91432" checksum="256B4E3A3391C6D9" modified="2018-12-05" version="1">MWNKLSTWQVLLWWHNFFILWSTIDAQTRYSIPEELKRGSVVGNIAKDLGLLVPELLRRKMRITSEAGKQYFNVDLGKGELLVTDRIDREELCGQRPSCLLPLELVIDNPLQVHRVEIEIQDTNDHSPSFLTKEKVVKIAELVNPGARFPLESATDPDVGANSVRTYLISKNEHFKLTVKNLKDGRKIPELVLEKALDRETQAVHNLVITAVDGGDPVRSGTSEITVIVLDNNDNAPQFEKQVYEANVSERSAIGTEILQVKASDADEGLNGEIEYVFAEQTSDIILSLFDINPSTGVITVKGVLDHESNSLHRFDITAKDKGSPEMDGHCGVEIKINDINDNNPEIVVTSLTTPVPEDSAIGTVIAIISTRDADSGDNGKVTLTLSPKSLFKLNPSISKHFSLVTNGPLDREKNSQYSIKITASDSGKPPLTSEKTIIVQLLDVNDNPPVFSQKLYTVYVKENNDPGMILCSVSASDLDSGENAKISYSILDSKVQDVSVSSYVYINSDNGSIYSMHSFDYEKLKVFQIQVQAKDQGSPSLSSNATVHVFILDQNDNTPAVIYPSSAALGSLSHQRMPRSAKAGHLVTKVTAVDADSGHNAWISYRLAEATDASLFTVNQYTGEVRTKRAVYEQDDSSQRLLIEIKDDGQPVQSATATVSILLEDGLHEPILDLRHKMSEPSKKTGRITLYLILSLASVSVLSLVTFLILAVKCIRGSRSSGSCCMRRSDCDDYKNPNRNLQIQLNTDGPIKYVEVLGGDMMSQSQSFRSCMSPMSEYSDFTLIKPSSTTDFKEVISVLDASLPDSTWTFESQQVTPHTGQRQLHNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2A0</accession>
    <name>A0A3B5L2A0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Myosin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2A0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006438"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006518.1">
        <property type="protein sequence ID" value="ENSXCOP00000006438.1"/>
        <property type="gene ID" value="ENSXCOG00000004988.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162888"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000101">
        <property type="entry name" value="Slow myosin heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
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        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF857">
        <property type="entry name" value="MYOSIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 8" id="KW-0518">Myosin</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="9">
        <location>
            <begin position="82"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="192" mass="22251" checksum="780E26A4B9D0C81A" modified="2018-12-05" version="1">VSAGVLEPEMECFGPAAIYLRKPERERIEAQNTPFDAKTAYFVSEPAEMYLKGKLVKKEGETKTLLTCNEDEIFPMNPPKFDKIEDMAMMTHLSEPSVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYDAMVVAGYRGKKRIEAPPHIFSISDNAYQFMLQGICYLFNCEHQTCHPVLCDNRSSWWKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2S5</accession>
    <name>A0A3B5L2S5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acylphosphatase</fullName>
            <ecNumber evidence="2">3.6.1.7</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an acyl phosphate + H2O = a carboxylate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
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            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the acylphosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="gene ID" value="ENSXCOG00000005126.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="entry name" value="Acylphosphatase-like_dom"/>
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        <property type="entry name" value="Acylphosphatase_CS"/>
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    </dbReference>
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        <property type="entry name" value="ACYLPHOSPHATASE 2"/>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="9"/>
            <end position="96"/>
        </location>
    </feature>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047645"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU004168"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="96" mass="10561" checksum="796E853C30A754F0" modified="2018-12-05" version="1">MSAGSGLQSVDFEIFGHVQGLFYTEKEGLRLGLVGWVKNTASGTVVGQVQGPAAMVEEMKFWLSKEGSPSSRITRAQFTNQKPIDKLEFSGFKTRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L3A2</accession>
    <name>A0A3B5L3A2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histidine rich calcium binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L3A2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002259"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002291.1">
        <property type="protein sequence ID" value="ENSXCOP00000002259.1"/>
        <property type="gene ID" value="ENSXCOG00000001808.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017281385" description="Histidine rich calcium binding protein" evidence="2">
        <location>
            <begin position="28"/>
            <end position="240"/>
        </location>
    </feature>
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        <location>
            <begin position="19"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="26951" checksum="B4B3871C39A55009" modified="2018-12-05" version="1">MAFIRSWVVALLLVLLSPLQLPHSGLAQDVAEEDEEEEEEDTRHAEEAEDEDGEGAEEDEELGEADEEETDEEEAEEEQEEGEEEEEGDEEQEEEAAEVDDESDGEEEEEDDDEEEDEASESDDGSEEEEDDEEDDEVADEVASEEEEEEDEAADDKVASDEEEEDHPAEDEEGTRCSEHCDKCPCAEGDESDHCERCEDCSSCYICPILCETVCTPGGFVDELTGSIFHNDEEVEREKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L455</accession>
    <name>A0A3B5L455_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
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            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L455"/>
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        <property type="protein sequence ID" value="ENSXCOP00000007103.1"/>
        <property type="gene ID" value="ENSXCOG00000005368.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SPRY_PRY_C-I_1"/>
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    </dbReference>
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        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
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    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="B30.2/SPRY"/>
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        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
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        <property type="entry name" value="Butyrophylin_SPRY"/>
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        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
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        <property type="entry name" value="PRY"/>
    </dbReference>
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        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
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        <property type="entry name" value="Znf-RING_euk"/>
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    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF32">
        <property type="entry name" value="BLOODTHIRSTY-RELATED GENE FAMILY, MEMBER 16 ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZF_RING_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="18"/>
            <end position="58"/>
        </location>
    </feature>
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        <location>
            <begin position="142"/>
            <end position="182"/>
        </location>
    </feature>
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            <begin position="336"/>
            <end position="530"/>
        </location>
    </feature>
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            <begin position="197"/>
            <end position="231"/>
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    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="262"/>
            <end position="289"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="544" mass="61715" checksum="8A1F0345663F087B" modified="2018-12-05" version="1">FEVMASHGLFLSKDQFQCCICQDVFSEPVSIPCGHSFCFTCITAHWEGRLSINCPKCHTEYKVCPELCENSFAKEMSEKIRAGRQDGVPGKTVGCDACVGKQTKALRSCLVCLTSYCESHLEPHLRVGSLKAHKLIEPVGMLENRVCRRHQELLELFCRSHQKFVCMQCVKTDHRCNDTVPVERESQDKKGQIKMIQGEVQQRIQDKLQKLEEIRRNLELSKENTKREIEESGQVFSALIRSIERSRAELVEVIQKKQAAAEMRARRLIAGLETEISELQRRKRELEQLFHHEDHLHVLFPAVSSLPSTEDSSNVLVHSETCLGTIRRAVADIRERLQLQLEKLSSRGLSDRQTDVLLDPRTANPWLVLSEDGRQVQDGDVMRNVADLPERFDKAPCVLALMGFTTGRRYWEVHVGGKTAWDLGVARASVRRKGAVTLSPDEGFWTVCLRKGAELWACAGEAELLCVSKRPKVIGVFLDYEDGVVSFYNAETGSHIYSFTGFHFTEAIFPFFNPDTNENGTNKLPSLCIYRHSTSTVRSFNYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4Z6</accession>
    <name>A0A3B5L4Z6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_028039431.1"/>
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    <dbReference type="STRING" id="32473.ENSXCOP00000005892"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005958.1">
        <property type="protein sequence ID" value="ENSXCOP00000005892.1"/>
        <property type="gene ID" value="ENSXCOG00000004576.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="RDD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14680:SF1">
        <property type="entry name" value="REQUIRED FOR DRUG-INDUCED DEATH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14680">
        <property type="entry name" value="SI:DKEY-126G1.9-RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF15828">
        <property type="entry name" value="RDD1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="134" mass="15235" checksum="99665590294BA928" modified="2018-12-05" version="1">MKPKSFPSRLFDRNSAGVSGERRNSCAAKYQRHVDSVDQPPSDGSSPNEKAKKDLNLAFLPERYEPLIDDEAKEEKKKRKKENYKKVKKNVGKALRTTWKCLMLGLYNFALAYSTPIAAAATFVPDFHPSRDRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L580</accession>
    <name>A0A3B5L580_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Janus kinase and microtubule-interacting protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the JAKMIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L580"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002956.1">
        <property type="protein sequence ID" value="ENSXCOP00000002919.1"/>
        <property type="gene ID" value="ENSXCOG00000002325.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:GABA receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="JAKMIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031994">
        <property type="entry name" value="JAKMIP_C"/>
    </dbReference>
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        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 4-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18935:SF6">
        <property type="entry name" value="JANUS KINASE AND MICROTUBULE-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Janus kinase and microtubule-interacting protein C-terminal" evidence="5">
        <location>
            <begin position="333"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="371"/>
            <end position="393"/>
        </location>
    </feature>
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            <begin position="145"/>
            <end position="347"/>
        </location>
    </feature>
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            <end position="460"/>
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            <begin position="491"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="596"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="371"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF16034"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="741" mass="86785" checksum="0498FE67FA3FE22D" modified="2018-12-05" version="1">MSGRKPEKSEVMADSPQATNEELRSKLMDIQIELQQERGKVCKLRERLQEQRQARELEQHKHAVALTDLRAKLHEEKLREISSAREALGRQHEAELTRAIKIRDTEVQRLQGLVNALRDGAADKLKNALLGEAREEARRAFDGERLKLQQEIQEQKTARKQAEEALANALQADKAKAADLRTAYQQHQDEVHRIKRDCEKDIRRLEVDERDMRRFQLKIAELHSVIRKLEDRNALLADERNELLKRVREAESQMKPMFEKNKRLSKKNDDLLQTLQRMEEKLKNLSRENAEMPQLKRPSSLTDLSHAHEEQEVEFLKLQVAEQRGIIEELTQERDRLVMNKKNRRKATKLPKRHVVETYFGFDEESIDSETSSLTSFNTDLTDRTPATPEEDLEETVSREESELRFRQLTREYQALQRAYALLQEQSGGTLDAEREARTREQLQMELSSCQAKIVDLEKALAESGQDSKWVEEKQYLIRTNQDLHEKICALQQAESRLQAEVQDARDQNELLEFRVLELEERERRSPALNLHMLAFPENSSSALQTYCHQEGVKDVIIPELMKKLDILGDNGNLRNEEQVAVIQAGTVISLCEKWLKQVDNTEAALTQKMIDLENDKELFSKQKGFLEEELDYRKQALDQAYMRIEELEATLYSALQQEQPACQAVAESLTDRQREELRLAVDKLRRQILRQSRQYDSQILQERMELLQQAQQRIRELEDRIDLQKRQIKEIEEKVQVQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L628</accession>
    <name>A0A3B5L628_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Immunoglobulin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L628"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003224"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003261.1">
        <property type="protein sequence ID" value="ENSXCOP00000003224.1"/>
        <property type="gene ID" value="ENSXCOG00000002540.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000270800"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05716">
        <property type="entry name" value="IgV_pIgR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF118">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Immunoglobulin" evidence="5">
        <location>
            <begin position="18"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="226"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="286" mass="32131" checksum="21F0E829D991DCB7" modified="2018-12-05" version="1">MIHTLTVLQLHCFFTQRLNKTQSIEEGSVTISCPYDSQSVNKLKFLCRGNRPSTCRQQAVITSRNNNKYSTNDDKRARTFTVTISDLRLDDAGKYWCGVTKDFTDIYKEVKLNIRPGTIESLLTSSISIWSGSSKAPDWKSLRRVVKTAEKLIGSPFPSIQNIADRRCLARANQILSDSTHPLHHLFIQLASGKRFRSICCRTAVPALPGWVFALCPPHLWAETGGQQTHQMHTSSATDPPQPHLPLENQDYQGTLRTCSPTLKPWRAVQADQPPLHPGSRLGLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6F4</accession>
    <name>A0A3B5L6F4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Unc-13 homolog D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">UNC13D</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the unc-13 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6F4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003354"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003392.1">
        <property type="protein sequence ID" value="ENSXCOP00000003354.1"/>
        <property type="gene ID" value="ENSXCOG00000002646.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000110939"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070382">
        <property type="term" value="C:exocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08676">
        <property type="entry name" value="C2A_Munc13-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052095">
        <property type="entry name" value="UNC-13_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45999:SF3">
        <property type="entry name" value="PROTEIN UNC-13 HOMOLOG D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45999">
        <property type="entry name" value="UNC-13-4A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="5">
        <location>
            <begin position="109"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="685" mass="79396" checksum="82EEEF265A8C7C9C" modified="2018-12-05" version="1">TMAGQSGQNTRKGENLLQTPEQVKKKTHFNKFSPVHTRKLGFTRRQRDRRDLKEDENPEAKARRHKEMELKPLYKELLYTIAHKMGKPASAEVFTNSQLHQYLKEAFTMTDEEHIALMEKVQNTEPPVYCLMVTVKEAKEILGKDVSGFSDPYCLLMVLEEEEEKKSSSAKAKPSKYVVKGTVSHEKIYQTDIKKQTLNPIWNQTFILEFVHLANTTFHLEMWDKDEEVSLTQKLEEIKTNLNSLRRMIKEAKKEKGTDDFLGNIVLRLQDLHCTEDNWYKLEPRTETYPNRGQCHLFLQFIHKARDGTLSAGRSAYTNYCGILQQFVQAYISKQQSSAPWKGALCGEAQTLLELYATQNDLSPFLQDLAKWVAYSKLYQTLEVDSSVLFHQLTSIEYHWHQQELPYQQKQELGDSLHGFLQYGLCLVSKYRDIFPPTPSATPKLHTLLRILVQICKTQAFQKQNPANFELHEGVHDAIETGTEEWFTIKKGLHQPMTKDLTEIGSALSRLIAEVREDIRHNKDVWNRVFFSAVQVDVFTVVYQKFDSLLAKEVKATLSIMEDQMDQNLSKSLFPVYLSLQAIHKDKAFLQKRGLLQLTDFHEGFRDALPYWLNQAFSTTQDRVVRAVQVDQVTRVGLKNMAGYLAIHKDSILKLLESRRGEREAQAFVKKRRQREKQSQEAVQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8J9</accession>
    <name>A0A3B5L8J9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NTCP5/P3 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bile acid:sodium symporter (BASS) (TC 2.A.28) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8J9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007147"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007239.1">
        <property type="protein sequence ID" value="ENSXCOP00000007147.1"/>
        <property type="gene ID" value="ENSXCOG00000005523.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182808"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008508">
        <property type="term" value="F:bile acid:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002657">
        <property type="entry name" value="BilAc:Na_symport/Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004710">
        <property type="entry name" value="Bilac:Na_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057103">
        <property type="entry name" value="NTCP5_P3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361:SF3">
        <property type="entry name" value="P3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361">
        <property type="entry name" value="SODIUM-BILE ACID COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24690">
        <property type="entry name" value="NTCP5_P3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01758">
        <property type="entry name" value="SBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0769">Symport</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="415"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="NTCP5/P3 N-terminal" evidence="9">
        <location>
            <begin position="28"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="444" mass="47578" checksum="1BB69A8CAF6433DF" modified="2018-12-05" version="1">RKTSLAFYAIKEVYIFVTDTSRTNHTGYISIGDGSAQEFEFPENTEGVIVISSRYRGSSRKQTVRVRSLDPEVLSVLNVTAGGLEGPAGSFIISIRSGFPGRAPLQIQLLDRDRDWDSVPVLIEERTDYFIRVEPSEADAATRLLQSGGLSHFTENPVLFALLPLIFVNKCAFGCKVEVEVLRALLRRPMALLLGVLAQFLVMPLYAYCASRLLSLPTALSLGLVITCSAPGGGGGYLYSLLLGGDVTLAISMTLVSTVVATAAMPLSSAVYGRLLGVHAALHVPFVKILGTLLFIAIPISLGMLIKLRLPALTRVLLVLIRPFSVVLMVGGIFMAYQMGASILANVQPQIVAVGVTVPLLGLLVGAGLARSAGLALPQMKTVSIEVGVQNSLLALAVMQLSFRRVEADFASQAPFIVALSSTSEMLLIVLGSFIWQRLCCPQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9C0</accession>
    <name>A0A3B5L9C0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Phytanoyl-CoA dioxygenase, peroxisomal</fullName>
            <ecNumber evidence="10">1.14.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Phytanic acid oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Phytanoyl-CoA alpha-hydroxylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>3-methylbutanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxy-3-methylbutanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54612"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57345"/>
            <dbReference type="ChEBI" id="CHEBI:138296"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:54613"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>3-methyldodecanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxy-3-methyldodecanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:55192"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:138636"/>
            <dbReference type="ChEBI" id="CHEBI:138637"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:55193"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>3-methylhexadecanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxy-3-methylhexadecanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:44000"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:58784"/>
            <dbReference type="ChEBI" id="CHEBI:83969"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:44001"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>3-methylnonanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxy-3-methylnonanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:55180"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:138633"/>
            <dbReference type="ChEBI" id="CHEBI:138634"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:55181"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>3-methylundecanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxy-3-methylundecanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:55184"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:84183"/>
            <dbReference type="ChEBI" id="CHEBI:138632"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:55185"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>butanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxybutanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:55176"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
            <dbReference type="ChEBI" id="CHEBI:138628"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:55177"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>decanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxydecanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54604"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61430"/>
            <dbReference type="ChEBI" id="CHEBI:138292"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:54605"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>dodecanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxydodecanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54628"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
            <dbReference type="ChEBI" id="CHEBI:138299"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:54629"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>eicosanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxyeicosanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54620"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57380"/>
            <dbReference type="ChEBI" id="CHEBI:138298"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:54621"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>heptadecanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxyheptadecanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54616"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:74307"/>
            <dbReference type="ChEBI" id="CHEBI:138297"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:54617"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>hexadecanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxyhexadecanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54596"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74115"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:54597"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>hexanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxyhexanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:55172"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
            <dbReference type="ChEBI" id="CHEBI:138630"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:55173"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>octadecanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxyoctadecanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54624"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:74116"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:54625"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>octanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxyoctanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54600"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57386"/>
            <dbReference type="ChEBI" id="CHEBI:138290"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:54601"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>phytanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxyphytanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:16065"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57334"/>
            <dbReference type="ChEBI" id="CHEBI:57391"/>
            <dbReference type="EC" id="1.14.11.18"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:16066"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tetradecanoyl-CoA + 2-oxoglutarate + O2 = 2-hydroxytetradecanoyl-CoA + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:54632"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:138300"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:54633"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="29">
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; fatty acid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PhyH family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.18" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9C0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004409"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004462.1">
        <property type="protein sequence ID" value="ENSXCOP00000004409.1"/>
        <property type="gene ID" value="ENSXCOG00000003479.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001775"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048244">
        <property type="term" value="F:phytanoyl-CoA dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001561">
        <property type="term" value="P:fatty acid alpha-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000012">
        <property type="entry name" value="Phytanoyl-CoA dioxygenase, peroxisomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047128">
        <property type="entry name" value="PhyH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008775">
        <property type="entry name" value="Phytyl_CoA_dOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21308">
        <property type="entry name" value="PHYTANOYL-COA ALPHA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21308:SF1">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0847">Vitamin C</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00051163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00051796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00051909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00051952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00052152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00052528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00052623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00053028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00071213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="300" mass="34754" checksum="865752FFD17350DA" modified="2018-12-05" version="1">MSRAAERLRTVIHHLDGATATIVSFRSVERSECSRTYSFDTGTLTPEQRLFYEENGFLLIKNLVSETDIDRFRYTFHFYVMNELRKTFIDIEPPLIQYCLGILKYVECFTGPNIMAMHTMLINKPPDAGDKTSRHPMHQDLHYFPFRPTDRIVCAWTAMERVTRQNGCLVVLPGTHKGALQEHDYPDWEGGVNKMYHGVRDYDPDHPRLHVEMEKGDTVFFHPLLIHGSGMNQTQGFRKAISCHYASGDCYYIDVRGTTQENIENEVKELAQKKYGMNEVAFKDTWAFRGRLVQGERTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9H1</accession>
    <name>A0A3B5L9H1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">BTB domain and CNC homolog 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">BACH1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9H1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007135"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007227.1">
        <property type="protein sequence ID" value="ENSXCOP00000007135.1"/>
        <property type="gene ID" value="ENSXCOG00000005514.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158923"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.880.10">
        <property type="entry name" value="Transcription factor, Skn-1-like, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004826">
        <property type="entry name" value="bZIP_Maf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF23">
        <property type="entry name" value="TRANSCRIPTION REGULATOR PROTEIN BACH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
        <property type="entry name" value="bZIP_Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BTB" evidence="6">
        <location>
            <begin position="26"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="BZIP" evidence="7">
        <location>
            <begin position="498"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="157"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="408"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="511"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="174"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="421"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="636" mass="69204" checksum="78983793689E1C15" modified="2018-12-05" version="1">MSVSSALHSAHVLQCLNEQRQQDILCDVTLLVEDQSFRAHCSVLASCSEYFRSRVTSVTRQNPIITLPEEVTVEGFEPLLQFAYTSKLLFTKENIHAILTSAEILGFRNLESGCFDFLIPKFSESKKTSQEVRSKSCCRSSEHGTSLDSCVEGSHAKSFESNSSAPSGGDEQTDFSSQCPQSTQGQTNSGDEHFCFQNCGPQMTPLSLELANDVCPLLSLPCPGPDKADHSSHFCEKDILQIGDVSDRSELRSVVGCDAQCELSTPGDENPPEITEPGSADNEPTVETLGAEASCAPASCPLKTSTAEDCTAVLQQDEVCPEQGAAGELSGPALNPQSLEAANGERSRVEREVAEHLAKGFWSDLCPGQSPLPSLDSASQNNLTKTSDFHWLRHLDLNSSVGDCPFLRDLDTDDEPGTRADNFSQAEKSPCMSSPLNSGDESELETDGDMEANHKRAAEVILPFPVEQISELSRSAFQQLVRDHSLTKEQLEFVHDVRRRSKNRVAAQRCRKRKLDSIHQLEYEIKKLKSEKEQLILEQKELKKNLAEIKQNVCGLCKSLLANLSSSDFPVSSPSLVANNMSQLSVEMVSSSLERGPIVPPLVSIQIAVPQSCTADTGCPVSAPSLSACLDMSNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCI5</accession>
    <name>A0A3B5LCI5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCI5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005579"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005645.1">
        <property type="protein sequence ID" value="ENSXCOP00000005579.1"/>
        <property type="gene ID" value="ENSXCOG00000004349.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156141"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11308">
        <property type="entry name" value="Peptidase_M14NE-CP-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1120:FF:000009">
        <property type="entry name" value="adipocyte enhancer-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000007">
        <property type="entry name" value="Carboxypeptidase X (M14 family), member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050753">
        <property type="entry name" value="Peptidase_M14_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532:SF43">
        <property type="entry name" value="CARBOXYPEPTIDASE X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532">
        <property type="entry name" value="PROTEASE M14 CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13620">
        <property type="entry name" value="CarboxypepD_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M14" evidence="14">
        <location>
            <begin position="1"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="13">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="449" mass="51520" checksum="12EA8D612BFF7396" modified="2018-12-05" version="1">EMRKLMKSVTEECPDITRIYTIGKSYTGLKLYVMEISDNPGKHELGEPEFRYVAGMHGNEALGRELVLNLMQYLCKEYKKGNQRVIRLVTETRIHLLPSMNPDGHEVAYKKGSELAGWAEGRYSYEGIDMNHNFPDLNNIMWDAQETAADPSKVSNHYIPIPEYYTQEDVAPETRAVISWMQDIPFVLSANLHGGELVVTYPFDCTRDWAPQENTPTADDAFFRWLATVYASTHLVLANPDRRICHYEDFQMHNNIINGGAWHTVPGMNDFSYLHTNCLEVTVELSCDKFPHASELPVEWENNKESLLVSSLTQVHRGIKGVIRDKSTKQGIENAVVKVEDHDHDIRSAADGDYWRLLNPGEYKVVVWAEGYFPSMRSCRVGLEPHPTICDFALTKTPIQRLKELRAKGEKIPRDLQLRLRALRMRKLRASTKAINRRRESQARRARSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCL7</accession>
    <name>A0A3B5LCL7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Progestin and adipoQ receptor family member VI</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCL7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005680.1">
        <property type="protein sequence ID" value="ENSXCOP00000005614.1"/>
        <property type="gene ID" value="ENSXCOG00000004375.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160567"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF39">
        <property type="entry name" value="MEMBRANE PROGESTIN RECEPTOR DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="363"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="403" mass="45797" checksum="7F1121D7539094E2" modified="2018-12-05" version="1">MKKSSRDLLSLFPVWRGIGASSAVGWSRGWGRCLRWEPGIWLVFCRRGRARLREVCQRGRGAIRLPTDLLSLKLPQLFDIHQVPKVFREDSIISGYRHPQSSAMDCVLSSFQMNNETVNIWTHFLPTWYFVWRFLALCSAMNFLNDSYTWPLLVYMLLICVYPFTSSCAHTFSSMSAESRHICYFFDYGALSLYSLGCAISYGHYVMPDCWVNSWLHQHFILIAIGNSLFCTSMSCYSRFVELQFPKRSKALRTGAFVVPFIFDTVPLFYRALICFWGGGTPSDALSSHCYHLLFAFLTCFLFTAHLPERLAPGRFDYIGHSHQLFHISAVVGTHFQMEGVVADMTSRRAWLQLHGATPSFLGTIGALAVSVALNLAIIGIFSAPLLWKTRPGSAQQHACREQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDP6</accession>
    <name>A0A3B5LDP6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Electron transfer flavoprotein subunit beta</fullName>
            <shortName evidence="8">Beta-ETF</shortName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase. Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism. ETFB binds an AMP molecule that probably has a purely structural role.</text>
    </comment>
    <comment type="function">
        <text evidence="8">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer composed of ETFA and ETFB. Identified in a complex that contains ETFA, ETFB and ETFRF1. Interacts with ACADM.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ETF beta-subunit/FixA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDP6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010288"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010411.1">
        <property type="protein sequence ID" value="ENSXCOP00000010288.1"/>
        <property type="gene ID" value="ENSXCOG00000007798.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009936"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045251">
        <property type="term" value="C:electron transfer flavoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000011">
        <property type="entry name" value="Electron transfer flavoprotein subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000049">
        <property type="entry name" value="ET-Flavoprotein_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294:SF8">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01065">
        <property type="entry name" value="ETF_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="9">
        <location>
            <begin position="25"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="237" mass="25558" checksum="7E2793E933EA0FC8" modified="2018-12-05" version="1">MSGRVLVGVKRVIDYAVKIRVKPDNTGVVTDGVKHSMNPFCEIAVEEAVKLKEKKLVKEVVAVSCGPQQSQETIRTALAMGADRGIHVEVSGKDYETLGPLQVSKIMAALAKKEDAQLIILGKQAIDDDCNQTGQMTAALLDWPQGTFASEVTLDGDKIKVVREIDGGLETIKINTPAVLTADLRLNTPRYATLPNIMKAKKKKIANVKAADLGVNLTSRLETVEDLVGKLKEAGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LG06</accession>
    <name>A0A3B5LG06_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LG06"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009787"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009905.1">
        <property type="protein sequence ID" value="ENSXCOP00000009787.1"/>
        <property type="gene ID" value="ENSXCOG00000007436.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000212973"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015269">
        <property type="term" value="F:calcium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005513">
        <property type="term" value="P:detection of calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003930">
        <property type="entry name" value="K_chnl_Ca-activ_BK_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10258">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10258:SF5">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT BETA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03185">
        <property type="entry name" value="CaKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="157" mass="17239" checksum="42A90FC0D7377D57" modified="2018-12-05" version="1">HMGMEMTTRGQGSRSFLLSLSLKGRVLSSMPRSPGVMTCSYSYGSDCGRVSRFPCLQVYVSVNNTGRIGRLSHNEEMQDASSEGSPCVYIPRCQKDSAANRAVIVNISQHLKTNQHLCLSHPVVALLHAQVGALIVVMVNLTQYLSRLCEEVGKLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHP8</accession>
    <name>A0A3B5LHP8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMEM70 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHP8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009206"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009317.1">
        <property type="protein sequence ID" value="ENSXCOP00000009206.1"/>
        <property type="gene ID" value="ENSXCOG00000007022.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018710"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009724">
        <property type="entry name" value="TMEM70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045325">
        <property type="entry name" value="TMEM70/TMEM186/TMEM223"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13281">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 70, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13281:SF0">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 70, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06979">
        <property type="entry name" value="TMEM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="245" mass="27622" checksum="06F85BF7746F3B8F" modified="2018-12-05" version="1">MDGSGDLWGLVFSLAVSPLRQPRFRCRSHRLRFLLLRLVLLGESAALWAAGSCLRSTPRSRKHSRVVVFMSPKSLDDRNSSRRWRSYANLLSGCKVCSVLGGVFWVKMFSYSTSGASLILMPQILLKTGLGLGNIALEVISCGIIGFFTFLTPVILHFITKGYVIRLYHNPDRDTYTAITYSVFLTEKRCVFHQSQVRIPAVSKMFANFYAGPMGLFVNPDLFPIPHDYNHLMGYDKLKTALMLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIJ2</accession>
    <name>A0A3B5LIJ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Diacylglycerol kinase</fullName>
            <shortName evidence="20">DAG kinase</shortName>
            <ecNumber evidence="20">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + ATP = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40327"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:40328"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1,2-didecanoyl-sn-glycerol + ATP = 1,2-didecanoyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43428"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18155"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:78227"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:43429"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + ATP = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40323"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:75728"/>
            <dbReference type="ChEBI" id="CHEBI:77091"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:40324"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:10273"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="19">Glycerolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; glycerolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIJ2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009471"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009582.1">
        <property type="protein sequence ID" value="ENSXCOP00000009471.1"/>
        <property type="gene ID" value="ENSXCOG00000007212.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159770"/>
    <dbReference type="UniPathway" id="UPA00230"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20845">
        <property type="entry name" value="C1_DGKbeta_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20891">
        <property type="entry name" value="C1_DGKbeta_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000017">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.40:FF:000003">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000013">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000016">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000003">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.110">
        <property type="entry name" value="Diacylglycerol kinase alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047471">
        <property type="entry name" value="C1_DGKbeta-like_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047470">
        <property type="entry name" value="C1_DGKbeta_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029477">
        <property type="entry name" value="DAG_kinase_typeI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038199">
        <property type="entry name" value="DGK_typeI_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF32">
        <property type="entry name" value="DIACYLGLYCEROL KINASE BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14513">
        <property type="entry name" value="DAG_kinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="EF-hand" evidence="24">
        <location>
            <begin position="127"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="24">
        <location>
            <begin position="172"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="22">
        <location>
            <begin position="222"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="22">
        <location>
            <begin position="286"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="DAGKc" evidence="23">
        <location>
            <begin position="407"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="645"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="380"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="651"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00060536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="777" mass="87552" checksum="2C0CB5F5AA4BB331" modified="2018-12-05" version="1">TTNQSQECLSAVNLLNKLKDVLQEFHGDGVLAKYNPEEEIDFEGFKVFMQTFLESELPEEFCQHLFMSFSNKGPRPSPSSADKPRVSGLKLIKSNSTPLKTSAPPGPLDMVQLKDIVFYLSLLEGGRPEDKLEFMFRLYDTDGNGSLDSSELEHIISQMMHVAEYLEWDVTELKPILQEMMHEIDYDQDGTVSLGEWIQGGLTTIPLLVLLGLETNVKDDGQHVWRLKHFNKPAYCNLCLNMLIGLGKQGLCCSFCKYTVHERCVARAPLSCIKTYVKSKKNTEVMHHFWVEGNCPTKCDKCHKTIKCYQGLTGLHCVWCQITLHNKCASHVKPECDCGPLKDHILPPNSICPVVLVRLRLFWSAGLEERQSTLRKDSRSSQSSRGKMQRANSVTVDGQGLQITTIEGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYNLAKNGPLPGLNFFRDVPDFRVLTCGGDGTVGWILDFIDKANLDRNPPVCILPLGTGNDLARCLRWGGGYEGENLLKILRDIENSTEVMLDRWKIDVTSADKEERGDPVPYSIMNNYFSIGVDASIAHRFHVMREKHPEKFNSRTKNKLWYFEFGTSETFSATCKKLHDFLEVECDGVTLDLSSISLEGIAILNIPSMHGGSNLWGESKKRRGQRKAGKKSQEKRTPVVDPKELMFAVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVTIRTTKSLPMQIDGEPWMQTPCTIQIVHKNQAHMLMGAPQGRSFFSSILRRTRSESKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJE9</accession>
    <name>A0A3B5LJE9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Myosin-binding protein H</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">H-protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Binds to myosin; probably involved in interaction with thick myofilaments in the A-band.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the immunoglobulin superfamily. MyBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJE9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009736"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009850.1">
        <property type="protein sequence ID" value="ENSXCOP00000009736.1"/>
        <property type="gene ID" value="ENSXCOG00000007404.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158040"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000557">
        <property type="entry name" value="Myosin binding protein Ha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000225">
        <property type="entry name" value="Myosin-binding protein C, cardiac-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000031">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000062">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF49">
        <property type="entry name" value="MYOSIN-BINDING PROTEIN H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="96"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="179"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="12">
        <location>
            <begin position="292"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="405"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="35"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="496" mass="53974" checksum="1981A8E21DDE4BFC" modified="2018-12-05" version="1">MPSKPAPMKKPPAKKAAEKAPEPAPEPEPTPPAEAPAEPALAAAEGEAAPATSADDSPAAEGAAPAKHVVKTGKLLPNACVFVFLIHSCRTVPSSAPLDVAVDDVNDTSLTIKWKTPENVGSSGLSGYTVEYRKDGTTDWIVANGELTMADCYCVRNLATGDLLHVRVVAVNPGGRSEPGLLPEPVPIREIGDRPKIRLPRLLRSCYVGKVGQQINLVLPFVGKPKPAVSWLKDGQPLDTKRVNIRNSDRDSIMFIRSAQREDSGVYEMTLKVESFEDKTTITLQIVDLPGPPASVKLVDAWGFNAALEWTPPKDNGNSDITGYTVQKADKKTGEWFTVLEHYHRLTATVSDLIMGNSYLFRVFSENQVGRSENGAVTKTAATIQKTGIVYKPPEYQEHDFNEAPKFTTPLINRAATVGYTTKLLCSVRGCPRPKIEWMKNQMIIGNDPKFRQISNQGICSLEIRKPCSFDGGVYTCRAKNAQGEAMVSCKLEVKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJZ8</accession>
    <name>A0A3B5LJZ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">XK-related protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the XK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJZ8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012408"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012559.1">
        <property type="protein sequence ID" value="ENSXCOP00000012408.1"/>
        <property type="gene ID" value="ENSXCOG00000009370.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282565"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018629">
        <property type="entry name" value="XK-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050895">
        <property type="entry name" value="XK-related_scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16024">
        <property type="entry name" value="XK-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16024:SF13">
        <property type="entry name" value="XK-RELATED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09815">
        <property type="entry name" value="XK-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="311"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU910716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="369" mass="42852" checksum="6C3E1075BBCAC7B3" modified="2018-12-05" version="1">MPYSKRRWLLALVGLFMYLGDICTDTALVFRYFHEKRYVWSGLTALFIITGLTVNQIFSYAWFLEDMHKNVMDSKPKRRFLVLHIFGMGILLRYVRALMSCIQDLQAANLNFISAAEMDSSTVDAADLSMLKLFETFLESAPQLLLQLYIRQSHDEWSVLQYMSMVFSFFNLAWSLVDYQIWLRKSLPQSRELPSGLPTVVYLLYKLFTITSLILSYSLLLFLSIYSTVGFTSLWLLGTIWAHLLQTDFCNSGVLELLYRGVIGAILVFTFFNAKGQNTKREMTVYYFFWTAINFSTPLLMFFLKPELKTITFFWVVSGVIYGGSLLGLISLVSYYRFLHPKQCELDQVDGQEKEKTPLTKRTRAFIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LK89</accession>
    <name>A0A3B5LK89_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-alpha-acetyltransferase 40</fullName>
            <ecNumber evidence="4">2.3.1.257</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">N-acetyltransferase 11</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">N-alpha-acetyltransferase D</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N-terminal L-seryl-[histone H2A] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[histone H2A] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50600"/>
            <dbReference type="Rhea" id="RHEA-COMP:12742"/>
            <dbReference type="Rhea" id="RHEA-COMP:12744"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.257"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N-terminal L-seryl-[histone H4] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[histone H4] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50596"/>
            <dbReference type="Rhea" id="RHEA-COMP:12740"/>
            <dbReference type="Rhea" id="RHEA-COMP:12743"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.257"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. NAA40 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.257" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LK89"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008304"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008403.1">
        <property type="protein sequence ID" value="ENSXCOP00000008304.1"/>
        <property type="gene ID" value="ENSXCOG00000006356.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014903"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043998">
        <property type="term" value="F:histone H2A acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010485">
        <property type="term" value="F:histone H4 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990189">
        <property type="term" value="F:protein N-terminal-serine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000033">
        <property type="entry name" value="N-alpha-acetyltransferase 40 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039949">
        <property type="entry name" value="NAA40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20531">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20531:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="16">
        <location>
            <begin position="12"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="139" mass="15672" checksum="E40E22758634B473" modified="2018-12-05" version="1">MNDERAWYLLARDADSTPLAFSHFRFDVECGEEVLYCYEAQLESKVRRKGLGKFLIQILQLIANSTQMKKVMLTVFKHNHGAYQFFREALQFEIDETSPSMSGCCGDDCSYEILSRRTKHGEASAGHTHGGGHCGGCCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKM9</accession>
    <name>A0A3B5LKM9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKM9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011225"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011356.1">
        <property type="protein sequence ID" value="ENSXCOP00000011225.1"/>
        <property type="gene ID" value="ENSXCOG00000008491.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183097"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051639">
        <property type="term" value="P:actin filament network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21333">
        <property type="entry name" value="CH_PLS2_rpt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21292">
        <property type="entry name" value="CH_PLS_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000042">
        <property type="entry name" value="Fimbrin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000010">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000012">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000014">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039959">
        <property type="entry name" value="Fimbrin/Plastin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961">
        <property type="entry name" value="FIMBRIN/PLASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961:SF35">
        <property type="entry name" value="PLASTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00019">
        <property type="entry name" value="ACTININ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="42"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="184"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="314"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="7">
        <location>
            <begin position="435"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="546" mass="61181" checksum="B038E63DF4B6490B" modified="2018-12-05" version="1">MVHGLKSTEVAKTFRKAINKKEGICNVAGTSEQSGTQHSYSEEEKVAFVNWINKALEKDPDCKHVIPMDPNNNDLFTAMGDGIVLCKMINLSVPDTIDERTINKKKLTPFTVQENLNLALNSASAIGCHVVNIGAEDLKEGRQHLVLGLLWQVIKIGLFADIEISRNEGIIVMVLLSLEDLMKLSPEELLLRWANYHLEEAGCGKINNFNSDIKDSKAYYNILNQVAPKGDEDGVPAIAIDMSGIREKEDLKRAECMLDQADKLGCRQFVMPADVVRGNPKLNLAFVANLFNKYPALKKPENQDIDWSSIEGETREERTFRNWMNSLGVNPRVNHLYADIDDALVIFQLYEKIKVPVDWDRVNKPPYPKLGSNMKKLENCNYAVELGKKEAKFSLVGIAGQDLNEGNRTLTLALLWQLMRRYTLNILEDLGDGQKVVDETIVSWVNGTLTEAGKGTISSFKDKSISSSMPVLDLIDAIQPGSIRYDLLKTEDLMEEEKLNNAKYAISMARKIGARVYALPEDLVEVKPKMVMTVFACLMARGMRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LL55</accession>
    <name>A0A3B5LL55_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="19">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 19">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(out) + ATP + H2O = a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66036"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:66037"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LL55"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011405"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011537.1">
        <property type="protein sequence ID" value="ENSXCOP00000011405.1"/>
        <property type="gene ID" value="ENSXCOG00000008609.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159531"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990531">
        <property type="term" value="C:phospholipid-translocating ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140351">
        <property type="term" value="F:glycosylceramide flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000006">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000022">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000009">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000130">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF79">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE VB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 18 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 19" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="292"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="944"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1006"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1041"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1066"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1107"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="20">
        <location>
            <begin position="42"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="21">
        <location>
            <begin position="895"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="16">
        <location>
            <position position="412"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="412"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="414"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="414"/>
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        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="559"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="601"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="625"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="667"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="747"/>
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        <ligand>
            <name>ATP</name>
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    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="748"/>
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        <ligand>
            <name>ATP</name>
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            <position position="749"/>
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        <ligand>
            <name>ATP</name>
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    </feature>
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        </location>
        <ligand>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
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            <position position="849"/>
        </location>
        <ligand>
            <name>ATP</name>
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    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="869"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="872"/>
        </location>
        <ligand>
            <name>ATP</name>
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    </feature>
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        <location>
            <position position="873"/>
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        <ligand>
            <name>Mg(2+)</name>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00050913"/>
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        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
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        <source>
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        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
            <dbReference type="Pfam" id="PF16212"/>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011405.1"/>
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        <source>
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        </source>
    </evidence>
    <sequence length="1217" mass="138455" checksum="123168FFD719C729" modified="2018-12-05" version="1">MTWRSPLALVRDALQGQRARERELRSLVSNLLYEGLEKTKQPNRFFLSNAIKTTKYSLLLFLPMNLYEQFHRFANLYFVGIAILNFIPVVNAFQPEVALIPICVILLLTALKDGWEDFRRYQSDRKVNNTPCFIYSRYKQYMERRWKDVRVGDFVRVVSNEIVPADLLLLYSSNPNGVCHIETANLDGETNLKQRTVVSGLWAANLGFEPESFNSVVVCERPNNNLNHFKCFVEKPDKDKVGASIESLLLRGCTVRNTNHAVGFVVYAGHETKSMLNSNGSRYKRSKLERKLNVDVFFCLLLLFTMCLIGAFGHYLWLESLPGVPSFLVPDSNGHLDVPSLESFYMFITMIILLQILIPISLYVSVELVKVGQIFFITNDIDLYDEETDNRVQCRALNITEDLGQIEYIFSDKTGTLTENKMVFRRCSVMGTEYPHKENGKEIYQKLLQQISRAESSGGRRDGNRNFDPHLDFFLALAICNTVVVSMENAHRQRVTGAFFQRNKWNRTRSEDPVIDSAKTENEADTNELQTCSLHPPSQNGSTIAEAEELCYEAESPDEAALVYAARAYGFILLARTPDSVTVRLPSGEDLVFEVLDTLTFDSNRKRMSVLVRHPVTKDYVLYTKGADYSIMELLGTPYAENVSGIEKNIAADTQYHLDCYAKDGLRTLCITKKVVSESTYQSWLGERKRAQAAMECREELIMRTAVQLETNLTLLGATGIEDRLQENVPDSIVALREAGIHVWVLTGDKPETAVNIGYACRLLEEEDLVINMSCKNKVTCTSNLDCTLEEVRRYGDDLHNIGTTQNISLVIDGNTLDIAMSPDLQQRFIELTKHCRSVLCCRVTPLQKSAVVKLVRDKLKVMTLAVGDGANDVNMIQAADIGIGISGQEGMQAVMASDFAISRFKHLKKLILVHGHWCYTRLANMIIYFFYKNVAYVNLLFWYQFFCGFTATTMFDYWQMIFFNLFFTSTPPIMFGIFDKDVSVEVLQGVPELYRTGQRKGEYNFLTFWISILDAFYQSLVCFFIPYLAYQDSDVDLFTFGMPLNTAALFTILLHLAIEIKSWTLVHWAIMLGSVALYFVVTLAYTAGCVTCNPPSNPYWILQSQMADPMFYLIVTLCTVVALLPRYSPPLCICHSVIYCRQLDRMDPSTRDQWIKEWRGFRGGAPAKRYELPDPPSPTLETPVDFFPDFLPDSDFMGDDLSLNVIAESRQRAVHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMP6</accession>
    <name>A0A3B5LMP6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FNBP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMP6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010786"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010911.1">
        <property type="protein sequence ID" value="ENSXCOP00000010786.1"/>
        <property type="gene ID" value="ENSXCOG00000008138.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183047"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11628">
        <property type="entry name" value="HR1_CIP4_FNBP1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000002">
        <property type="entry name" value="Formin-binding protein 1-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057871">
        <property type="entry name" value="HR1_CIP4_FNBP1L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057870">
        <property type="entry name" value="HR1_TOCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF14">
        <property type="entry name" value="FORMIN-BINDING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25610">
        <property type="entry name" value="HR1_TOCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 14 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="18">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="19">
        <location>
            <begin position="346"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="498"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="293"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="418"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="131"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="353"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="293"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="305"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="418"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="472"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="568" mass="66178" checksum="5106B5FB43A227E3" modified="2018-12-05" version="1">MNDLTKLWFSDQFENLDKHTQWGIDFLERYAKFVKERLDIEQNYAKQLRNLVKKYCPKRSKDEEPRFTSCMSFYSIMGELNDYAGQREVVAEEMSHKVYNELMRYSQELKAERKHHLQEGRKAQQFLDHCWKQMDNSKKKFERECREAEKAQITYDRLDNDINATKSEVEKAKAQLYTRTHMADESKNEYAAQLQNFNGEQWKHFNNSIPQIFKNLQGMDERRTVKLGETYRSFAEAERRVIPIITKCLDGMVSAAKAVDEQRDSTIVVESFKSGFEPPGDFPFEDFSQNLSRTGSDGTISNTPKGERELAPVPRSEAKHTISKGKNKLWLFGKKPKIPSLEDFSHLPPEQRRKRLQQKIDELNRELQKETDQRDALNKMKDVYEKNPQMGDPNNLQPKISETNCNIQKLRSEIHKNETWLSEVEGKQSSRGDRRHSADNHHHTPHGRESPEGSYNDDTSQEHQTPHHTPQHRPAQPAQPNHDPHEFDDEFDDDDPLPVIGHCKALYSFHAAMLGMLLDLLQVLYIIEEDKGDGWTRARKQSGEEGYVPTSYVEITIEKNSKGAVTYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMR1</accession>
    <name>A0A3B5LMR1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Presynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMR1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012005"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012143.1">
        <property type="protein sequence ID" value="ENSXCOP00000012005.1"/>
        <property type="gene ID" value="ENSXCOG00000008036.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164903"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032590">
        <property type="term" value="C:dendrite membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032809">
        <property type="term" value="C:neuronal cell body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18414">
        <property type="entry name" value="BTB_KCNC1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000011">
        <property type="entry name" value="Potassium channel, voltage-gated Shaw-related subfamily C, member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000014">
        <property type="entry name" value="Potassium channel, voltage-gated Shaw-related subfamily C, member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000002">
        <property type="entry name" value="Potassium voltage-gated channel subfamily C member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003974">
        <property type="entry name" value="K_chnl_volt-dep_Kv3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF184">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY C MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01498">
        <property type="entry name" value="SHAWCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="7" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0770">Synapse</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="384"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="413"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="20">
        <location>
            <begin position="15"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="639" mass="71235" checksum="B5646746332A150A" modified="2018-12-05" version="1">EGMDNPMTCSASDSEKIVINCGGIRHETYRSTLKTLPGTRLSWLTEPDAFSNFDYDPKSDEFFFDRHPATFAFILNYYRTGKLHCPNDVCGPLFEEELAFWGIDETDVEACCWMNYRQHRDAEEALDSFETPEPDVPEDDPALTGGVDGDLKRLCMQEDGRKATWWEVWQPRMWALFEDPYSSKYARYVALASLFFILLSISTFCLETHEAFNTIYNKTENVTTGNVTHEEIVYEVVTDGWLTYVEGVCVIWFTIEVVARVIFCPDKAEFFRSTLNIIDFVAIVPFYLEVALSGLSSKTAKDVLSFLRVVRFVRILRIFKLTRHFVGLRVLGHTLRASTNEFLLLIIFLALGVLIFATMIYYAERIGADPKDPTAAAHTNFKNIPIGFWWAVVTMTTLGYGDMYPETWSGMLVGALCALAGVLTIAMPVPVIVNNFGMYYSLAMAKQKLPKKRNKHIPRAPQPGSPNYCKPDALAMATASPHRLMGNVIGPGLVESGSMIGTGDCPLAQEEIIEINRADSKQNGDAAANAAALANEDCPTIDQVLGDERSPVTGGLGSNTSRERYPHDRACFLLSAGEFQRTTDGNVRKVTGYEKSRSLNNISGMTGGPLRLTPITNSTFETFEPSGLRRCHSPIPSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMR5</accession>
    <name>A0A3B5LMR5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="33">Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating</fullName>
            <ecNumber evidence="32">1.1.1.170</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="36 37">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="36" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="36" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>4alpha-carboxy-4beta-methyl-5alpha-cholest-8-en-3beta-ol + NAD(+) = 4alpha-methyl-5alpha-cholest-8-en-3-one + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:47168"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:87047"/>
            <dbReference type="ChEBI" id="CHEBI:87050"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:47169"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>4alpha-carboxy-4beta-methyl-5alpha-cholest-8-en-3beta-ol + NADP(+) = 4alpha-methyl-5alpha-cholest-8-en-3-one + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:46828"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:87047"/>
            <dbReference type="ChEBI" id="CHEBI:87050"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:46829"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>4alpha-carboxy-5alpha-cholest-8-ene-3beta-ol + NAD(+) = 5alpha-cholest-8-en-3-one + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:47172"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:87055"/>
            <dbReference type="ChEBI" id="CHEBI:87056"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:47173"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>4alpha-carboxy-5alpha-cholest-8-ene-3beta-ol + NADP(+) = 5alpha-cholest-8-en-3-one + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:46848"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:87055"/>
            <dbReference type="ChEBI" id="CHEBI:87056"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:46849"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>4alpha-carboxyzymosterol + NAD(+) = zymosterone + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:47164"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:52386"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:143575"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:47165"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>4alpha-carboxyzymosterol + NADP(+) = zymosterone + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:33455"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:52386"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:143575"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:33456"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>4beta-methylzymosterol-4alpha-carboxylate + NAD(+) = 3-dehydro-4-methylzymosterol + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:47160"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:50593"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64925"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:47161"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>4beta-methylzymosterol-4alpha-carboxylate + NADP(+) = 3-dehydro-4-methylzymosterol + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:33447"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:50593"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:64925"/>
            <dbReference type="EC" id="1.1.1.170"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:33448"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>a 3beta-hydroxysteroid-4alpha-carboxylate + NAD(+) = a 3-oxosteroid + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:34775"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:47788"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:136966"/>
            <dbReference type="EC" id="1.1.1.170"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a 3beta-hydroxysteroid-4alpha-carboxylate + NADP(+) = a 3-oxosteroid + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:34771"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:47788"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:136966"/>
            <dbReference type="EC" id="1.1.1.170"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="31">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 4/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 34">Belongs to the 3-beta-HSD family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.170" evidence="32"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMR5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012010"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012148.1">
        <property type="protein sequence ID" value="ENSXCOP00000012010.1"/>
        <property type="gene ID" value="ENSXCOG00000009088.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158229"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000252">
        <property type="term" value="F:3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000251">
        <property type="entry name" value="Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050177">
        <property type="entry name" value="Lipid_A_modif_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN ARNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245:SF51">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE_REDUCTASE FAMILY 42E, MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01073">
        <property type="entry name" value="3Beta_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0152">Cholesterol biosynthesis</keyword>
    <keyword evidence="6" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="17" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="18 34" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0520">NAD</keyword>
    <keyword evidence="14 34" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="37" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="20" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="15" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="19" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="8 34" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 34" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="3-beta hydroxysteroid dehydrogenase/isomerase" evidence="35">
        <location>
            <begin position="36"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022778"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022955"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00051034"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00051208"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00066634"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00074569"/>
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    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="RuleBase" id="RU004475"/>
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    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="Pfam" id="PF01073"/>
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    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012010.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="368" mass="41241" checksum="76A9752750AED387" modified="2018-12-05" version="1">LQNKQVQKWRIGWMVTHLSEITLMGNDERKGSKRCAVIGGSGFLGRHLVEKLLDRGYSVSLFDIRQSYELPGVTFHQGDLCDKQALLSALKDVSLVFHCASPAPSSDDRLLFERVNIQGTQTVIQACLDAGVQKLVLTSSASVVFEGTDVKNGKEDLPYAKKPIDYYTQTKIEQEKLVLEACNKPKGFLTVAIRPHGIFGPRDPQLVPTLVDAARRGKMKFIIGDGTNLVDFTYVENVVHGHILAAEHLRADSPICGKPYHITNDEPIRFWDFMSEILVGLGYAAPRFHLPYTLVYGLALLLWLLTWILHPFMSFKPTFTPMRVALAGTYHYYSCERAKEHLGYKPVVSLKEGIARTVASYPHLRRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LN71</accession>
    <name>A0A3B5LN71_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein phosphatase 1 regulatory subunit 37</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Leucine-rich repeat-containing protein 68</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the PPP1R37 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LN71"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012588.1">
        <property type="protein sequence ID" value="ENSXCOP00000012437.1"/>
        <property type="gene ID" value="ENSXCOG00000009397.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
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        <property type="entry name" value="Leu-rich_rpt"/>
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        <property type="entry name" value="LRR_dom_sf"/>
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        <property type="entry name" value="PP1-Reg/Actin-Interact_Protein"/>
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        <property type="entry name" value="LEUCINE-RICH REPEAT, ISOFORM F-RELATED"/>
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        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 37"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="295" mass="32589" checksum="AD019F58130354B6" modified="2018-12-05" version="1">SLLDMILYYESTTYLDISDNGSMGTSSWRALAHLIKQSVCLSRLDACNVCLVDYPVQSLSKALLTSRLQVLHLNNVQLSGSSLFTLCALKINRALLELHLSSNQLNGYQDAMQLGDLLRYNSTLQTLELSDNVLGDALEELCDGLSRQTKGLKVLILKNNQITERGMNHLAKTLLQPGLKILEVLDLGDNHLGNEGIQVIREPLMVNSSLLHLGLAQSNITCEAVALAEFLAESRLIQRLDLHQNRVKLGGLMALSLALRINCSLSHLGDEFLSETQKRLQAEITQRCKANASRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNM6</accession>
    <name>A0A3B5LNM6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">WWC family member 3</fullName>
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    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNM6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011051"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011178.1">
        <property type="protein sequence ID" value="ENSXCOP00000011051.1"/>
        <property type="gene ID" value="ENSXCOG00000008344.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of organ growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035330">
        <property type="term" value="P:regulation of hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08680">
        <property type="entry name" value="C2_Kibra"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1"/>
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    </dbReference>
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        <property type="entry name" value="WW and C2 domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="C2_WWC"/>
    </dbReference>
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        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051105">
        <property type="entry name" value="WWC/KIBRA_Hippo_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057747">
        <property type="entry name" value="WWC1_hairpin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14791">
        <property type="entry name" value="BOMB/KIRA PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14791:SF25">
        <property type="entry name" value="PROTEIN WWC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25802">
        <property type="entry name" value="WWC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="WW" evidence="10">
        <location>
            <begin position="15"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="10">
        <location>
            <begin position="62"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="679"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="371"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="512"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="607"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="819"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="995"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="308"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="932"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="393"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="526"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="821"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1000"/>
            <end position="1010"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1058" mass="119330" checksum="6A0847A50A44EE18" modified="2018-12-05" version="1">MPWVSGGKRRESSELPLPAGWEEARDYDGRVFYIDHNTRQTSWIDPRDRITKPLTFADCVGDELPLGWEEVYDQQVGVYYIDHINKTTQIENPRTQWRQEQERMLKEYLVVAQEALTAKKEMYLIKQQRLELAQQEMLRLDELSGDTHSITSTFSGSSASSKYDPDQIKMEIACKRERLSRLKQELAQVKQELQYKEMGVETLQEIDRKMSCSQTNYKLDEAQAIFNELRSIKKSISTGEKERQDLIQSLAKLTVHFQTSLSVSDTAGEVADSSATAGDQCYPQQYCDTGCQTDIMGEVRAPSHLVDKVKLNWQYEEAKKKVQSIQHQLAQLDSESWSGRAEADRDRDFLQLLREKEALLQELILVSKKPHAAEKQQQLEEERSRLEEEVQKAHSSQSQGANQRLLQQEKRNDLLRQLQEATRITTYLHSQLKSLSASSLTVSSSSSRGSLASSRGSLASSRGSLSSISFSDIYGVPQYDRHEGAAEALDPHLRYLLPPESASRDCSVFNPEHMTQSKSKRSHDTPQSLASLSSRSSLSSLSPPSSPMDTPYHLAPQDCPLAQMTEEYMEQAGRGLLEGLRAQTVSHGVLMGGEDTVSPAALHQLDSKSQREGGAQTAFTSTGVTLRGNSANRSGRRARRVSAGVSEDALATDSGVFEAWGKRPEEYDEVSYAKEASAASEPTQIQLGLLWESASQSLRLHVLQLKSLNCSNVRESYKVYVKVHLIPLDASRACAFYCCTALEPQSQMTFNEGFRIPVPTNALAVCALQLSVCSLGPQAQEELLGTAKLSLADCEGSADMVYHWLRVQMLTDSQKALYSRRTHDSQEDEARPRATVNGHSHADEQRLAKLFTPFDLFCIKATYFSSSGQSKNPRITPTRKMTRKAEHPVRLYCGVSFTVKVILHLLTRGTAVCLNTQPSYRSSLAEQPTRTSLDLELDLQACRTRLRQLMEELSALRELKLRLEEPQASDADELPTWALRDERFRCLLREAQRQANQSKQEQRQEEAAERRLRKASKEVLQMRGQNQKEPLPVQTFREKMAFFTRPRFNIPPLPADDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNQ9</accession>
    <name>A0A3B5LNQ9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LDAF1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNQ9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013718"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013886.1">
        <property type="protein sequence ID" value="ENSXCOP00000013718.1"/>
        <property type="gene ID" value="ENSXCOG00000010379.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175471"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029709">
        <property type="entry name" value="LDAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14275:SF0">
        <property type="entry name" value="LIPID DROPLET ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14275">
        <property type="entry name" value="PROMETHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16015">
        <property type="entry name" value="Promethin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="142" mass="16207" checksum="DCC12F1659FA1F85" modified="2018-12-05" version="1">CVYKKVQLVSLDSQIKKKNLLRTMNLSERFLWLIFRPFLALALLLFSAMAALPVGVFLLFALITIVMSVVGFVFFEGMLHISNSTMWGFFSSYFKLILILLFSVSAVYWRNDSAVSPVFCCWLFQASVAVVHETPQRHLCTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNS4</accession>
    <name>A0A3B5LNS4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNS4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011223.1">
        <property type="protein sequence ID" value="ENSXCOP00000011096.1"/>
        <property type="gene ID" value="ENSXCOG00000008384.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177606"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007098">
        <property type="term" value="P:centrosome cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090063">
        <property type="term" value="P:positive regulation of microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903358">
        <property type="term" value="P:regulation of Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052593">
        <property type="entry name" value="MT-associated_AKAP9-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46501">
        <property type="entry name" value="MYOMEGALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46501:SF2">
        <property type="entry name" value="MYOMEGALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="144" mass="16419" checksum="9499213EA8A76FB9" modified="2018-12-05" version="1">SSSSEVGAIWQDIEEGLREQAARLRSDLTLSLQENRELKERLMVSEATVHAQAEQLKDYRDLSVIRLRKLLSLFTQRFTSNLRASLPLLRKYNSLIQAQARELSHLRQRMREGQGVCHILTQHLGDTTKVLQLPIGTGVTAKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPV3</accession>
    <name>A0A3B5LPV3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Threonylcarbamoyl-AMP synthase</fullName>
            <ecNumber evidence="3">2.7.7.87</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonine + hydrogencarbonate + ATP = L-threonylcarbamoyladenylate + diphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUA5 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011456"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011588.1">
        <property type="protein sequence ID" value="ENSXCOP00000011456.1"/>
        <property type="gene ID" value="ENSXCOG00000008647.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015364"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.870.10:FF:000010">
        <property type="entry name" value="Si:ch211-153b23.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="Sua5-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050156">
        <property type="entry name" value="TC-AMP_synthase_SUA5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490:SF17">
        <property type="entry name" value="THREONYLCARBAMOYL-AMP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="YrdC-like" evidence="9">
        <location>
            <begin position="87"/>
            <end position="856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="869" mass="95778" checksum="FB039DA17C46B68C" modified="2018-12-05" version="1">SLLLLVNSYASTPEKDFIPHTALGILLLIIAALIAYTVRHSLSHSRLFSTLCLTISALWCGSGLVYILIGQRVLPLTELKSSLIPGLAAFTLALFIIGIAALFVKKPVLLVIAISISXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGFRVKKQAEDRPMSMWISSVKQLEPVRHLLSPLLLDFMESAWPSSISMVIPRGPWLDIFGLGDAAKHIGTPQSIAIRSPDCAVATHLINLVGPIAVTSANPTGEADTTHHNQVYAKLGKKVEGVLCDGPSPENIASTVVDCTKIETGQIGFFRVGLIPKSKVLQIFEEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQX2</accession>
    <name>A0A3B5LQX2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Collagen IV NC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQX2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011806"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011942.1">
        <property type="protein sequence ID" value="ENSXCOP00000011806.1"/>
        <property type="gene ID" value="ENSXCOG00000008925.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164076"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005201">
        <property type="term" value="F:extracellular matrix structural constituent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.240.10:FF:000001">
        <property type="entry name" value="Collagen IV alpha 1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.240.10">
        <property type="entry name" value="Collagen IV, non-collagenous"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001442">
        <property type="entry name" value="Collagen_IV_NC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036954">
        <property type="entry name" value="Collagen_IV_NC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019326">
        <property type="entry name" value="NDNF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14619:SF8">
        <property type="entry name" value="COLLAGEN TYPE IV ALPHA 4 CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14619">
        <property type="entry name" value="NEURON-DERIVED NEUROTROPHIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01413">
        <property type="entry name" value="C4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00111">
        <property type="entry name" value="C4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51403">
        <property type="entry name" value="NC1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="6" id="KW-0176">Collagen</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Collagen IV NC1" evidence="9">
        <location>
            <begin position="199"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="416" mass="43484" checksum="A5FADE4FFB45FA35" modified="2018-12-05" version="1">FPGPAGMKGTPGFPGENGGTGPRGPPGLPGPGTIPGRPGTPGRKGDKGLPGLPGRPGSPGRPGFPVDSKGQAGEPGFPGRPGQPGFPGTKGEGGIMGFPGVTGERVRRNSPVPHISVQHNQGGGAEGRPGAAGELGRTGPPGFPGPVGDPGFPGSQGITGDQGQETLATIWSRFSLVGFPGAIGRPGIPGGVGRSIGVGYTLVKHSQTTDVPMCPQGMDQLWEGYSLLDFSLKLCQPGSCLPRFNTIPFLYCNPSETCYYASRNDKSFWLSTTAPIPMMPVAEEQIRPYISRCSVCEAPSQAVAVHSQDLTLPNCPPGWRSLWIGYSFLMHTAAGAEGGGQSLMSPGSCLEDFRSTPFIECNGARGTCHYFASKYSFWLTTVDPRLEFGAPTQQETLKGGQERFRVSRCQVCSKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSK9</accession>
    <name>A0A3B5LSK9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">glycogenin glucosyltransferase</fullName>
            <ecNumber evidence="11">2.4.1.186</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Glycogenin participates in the glycogen biosynthetic process along with glycogen synthase and glycogen branching enzyme. It catalyzes the formation of a short alpha (1,4)-glucosyl chain covalently attached via a glucose 1-O-tyrosyl linkage to internal tyrosine residues and these chains act as primers for the elongation reaction catalyzed by glycogen synthase.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Self-glucosylating initiator of glycogen synthesis. It catalyzes the formation of a short alpha (1,4)-glucosyl chain covalently attached via a glucose 1-O-tyrosyl linkage to internal tyrosine residues and these chains act as primers for the elongation reaction catalyzed by glycogen synthase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-tyrosyl-[glycogenin] + UDP-alpha-D-glucose = alpha-D-glucosyl-L-tyrosyl-[glycogenin] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23360"/>
            <dbReference type="Rhea" id="RHEA-COMP:14604"/>
            <dbReference type="Rhea" id="RHEA-COMP:14605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:140573"/>
            <dbReference type="EC" id="2.4.1.186"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:23361"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[1,4-alpha-D-glucosyl](n)-L-tyrosyl-[glycogenin] + UDP-alpha-D-glucose = [1,4-alpha-D-glucosyl](n+1)-L-tyrosyl-[glycogenin] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56560"/>
            <dbReference type="Rhea" id="RHEA-COMP:14606"/>
            <dbReference type="Rhea" id="RHEA-COMP:14607"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:140574"/>
            <dbReference type="EC" id="2.4.1.186"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:56561"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 8 family. Glycogenin subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.186" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSK9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015003"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015192.1">
        <property type="protein sequence ID" value="ENSXCOP00000015003.1"/>
        <property type="gene ID" value="ENSXCOG00000011358.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154674"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008466">
        <property type="term" value="F:glycogenin glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02537">
        <property type="entry name" value="GT8_Glycogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000092">
        <property type="entry name" value="Glycogenin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017474849" description="glycogenin glucosyltransferase" evidence="17">
        <location>
            <begin position="21"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="334" mass="37572" checksum="3F310D5678C213F2" modified="2018-12-05" version="1">MGRTWWIYFVNLAQAFVTLATNDSYARGAMVLGKSLRNHNTSKKLVVLVGPQVSNPCQSVLKNIFDEVKVVDVLDSGDAAHLAMMKRPDLGVTFTKLQCWTLTHYSKCVFMDADTLALSNIDELFEREELSAAPDPGWPDCFNSGVFVFRPSVETHGKLLQYCTEHGSFDGGDQGVLNGFFSDWATADISKHLPFIYNLSSIAIYTYLPAFKQYGGNAKVLHFLGKTKPWSYTFDRKTRQISGGVQEPAMHPSFLLNWWTLYASTVVPMLQEHYGDKPFHSGCVEGESGSSHAQAFQPIPTSEERKEQWEQGQADYMGMDSFDNIKRKLDTFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSL1</accession>
    <name>A0A3B5LSL1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SWI/SNF Subunit INI1 DNA binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSL1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013805"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013974.1">
        <property type="protein sequence ID" value="ENSXCOP00000013805.1"/>
        <property type="gene ID" value="ENSXCOG00000010451.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000038585"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21086">
        <property type="entry name" value="WH_NTD_SMARCB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048664">
        <property type="entry name" value="INI1_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006939">
        <property type="entry name" value="SNF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10019">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21459">
        <property type="entry name" value="INI1_DNA-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04855">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SWI/SNF Subunit INI1 DNA binding" evidence="6">
        <location>
            <begin position="10"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF21459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="266" mass="30538" checksum="C09978B9AA468336" modified="2018-12-05" version="1">MALSKTFGQKPVKFQLEEDGEFYMIGSEVGNYLRMFRGSLYKRYPSLWRKLATVEERKKIVESSHGQLRSVTKNHGYTQLATSVTLLKASEVEEILEGNDEKYKAVSISTEPPTYLREQKAKRNNQWVPTLPNSSHHLDAVPCSTTINRSRLGRDKKRTFPLCFDDHDPAVIHENATQPEVLVPIRLDMEIEGQKLRDAFTWNMNEKLMTPEMFAEILCDDLDLNPLAFVPAIASAIRQQIESYPTDSLLEDQTDQRVIIKVRLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSW5</accession>
    <name>A0A3B5LSW5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Orange domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSW5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013915"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014085.1">
        <property type="protein sequence ID" value="ENSXCOP00000013915.1"/>
        <property type="gene ID" value="ENSXCOG00000010537.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159619"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00511">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Orange" evidence="6">
        <location>
            <begin position="100"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="246" mass="25983" checksum="A194F9FFCEFE7AE8" modified="2018-12-05" version="1">GLTRHGEHPRAGPHTRAPRGSAQTARATSFGTRKGQQTHPPASFASLLQPTVKRGRCGNHPPPCIGALSLSLPFGERSAQDALRHPSTALNTDPSILGKYRAGFSECMNEVTRFLSTCEGVNTEVRTRLLGHLASCMTQINAMNYPSQHQHQHQLPPAVLPMNPASCKGGSPPASLPSDATKVYGGFQIVPATDGQFAFLIPNAAFAPNGPVIPVYANNMSTSVPVPAAVSPGAPSANTDSVWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LU24</accession>
    <name>A0A3B5LU24_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transforming acidic coiled-coil-containing protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TACC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LU24"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014350"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014534.1">
        <property type="protein sequence ID" value="ENSXCOP00000014350.1"/>
        <property type="gene ID" value="ENSXCOG00000010885.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157052"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021987">
        <property type="term" value="P:cerebral cortex development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1700:FF:000001">
        <property type="entry name" value="Transforming acidic coiled-coil-containing protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039915">
        <property type="entry name" value="TACC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007707">
        <property type="entry name" value="TACC_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13924">
        <property type="entry name" value="TRANSFORMING ACIDIC COILED-COIL CONTAINING PROTEIN 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13924:SF11">
        <property type="entry name" value="TRANSFORMING ACIDIC COILED-COIL-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05010">
        <property type="entry name" value="TACC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transforming acidic coiled-coil-containing protein C-terminal" evidence="9">
        <location>
            <begin position="608"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="103"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="284"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="593"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="103"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="219"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="302"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="352"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="365"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="422"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="466"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="484"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="757" mass="84301" checksum="FEF03C93E36AD9E8" modified="2018-12-05" version="1">MFCFRSSDSDGAFETPESTTPVKAVSPAEPQRELLESDDKGVNGSEGDLSLDITATDAPCRSPSIAFDENKPIAASGTYNFDFVAVEPTSHTLTRSLSLQGGELDSSSLLEGSASGAFRQHSESFSIGTENTPGKLRRPKKVSPGSVKKKPFLRQNSNPDSSKPASSKETNQTCQEESKGVPALPLCQEEAPLAENVEDKDNSPIPPSASYKWDPENFENINPFSTGGSKITNSPVLGHKGPVLPVPAAEPHHHTVTAPLEELSTNPEEQPILPKRQPVRLEFDYSEESCEVPQQASPPSKKLGKKPPGKMLSRKPKLGLKKVAPQQVEQLDNNPPAAYNGSDEIPIPKVSYHIEPDKWDDPNFNPFSSKKSMSNSPTLSRPSYSFDPNNIVESTNPFKSSNKMAASPPRVPVSFETSSNDYDNENDNDNIGELEDQNQNKPAKKKKTPLKSNTFRVKRSPKKSPLSDTSQDPTSADESSSLHQQDDHATDEEKLASSTGHKWAVRHEVDQDLNTDHQDFPQPCDVTTFVNENSLPQENPGLALLSDFFLSPSGTCVHATSKCTTLVQLFSEIQMAKVISFQHLELYLKEKEVLEWQRKYEDSRQEVDDQKEKSLSHHTIQQLIIEKDQALADLNSVEKSLADLFRRYEKMKDVLEGFRKNEEVLKKCAQEYLSRVRKEEQRYQALKIHAEEKLDKANAEIAQVRSKAKQEQAAHQASLRKEQMKVDSLERTLEQKNKEIEELTKICDELIAKMGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUE2</accession>
    <name>A0A3B5LUE2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DnaJ homolog subfamily C member 17</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May negatively affect PAX8-induced thyroglobulin/TG transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUE2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014762"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014948.1">
        <property type="protein sequence ID" value="ENSXCOP00000014762.1"/>
        <property type="gene ID" value="ENSXCOG00000011198.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155132"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12429">
        <property type="entry name" value="RRM_DNAJC17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000059">
        <property type="entry name" value="dnaJ homolog subfamily C member 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034254">
        <property type="entry name" value="DNAJC17_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052094">
        <property type="entry name" value="Pre-mRNA-splicing_ERAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44313">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44313:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="J" evidence="10">
        <location>
            <begin position="12"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="188"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="73"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="311" mass="35446" checksum="7F65BE902EFF1C6A" modified="2018-12-05" version="1">MSGKAKEILQMDLYGLLGIGSSATTKEIKKAYRQKALTCHPDKNPDNPKAAELFHQLSQALEVLTDAAAKAAYDKICAAKKQAEERNKKLDDKRKKIKLDLEARERRAEVQSQEEVQITRTLEEEIARLREEGSRQLEEEQRLIREQIQREREAHCSFVFLPHWERYSQSNATPKLKLKWKCKKDDELNGGYSQDILLKLLHKYGDVLNVIVSSKKKGSAVVEFASVRAAELAVKNESGLSGNPLKITWLEGQPEDVAAASQTGHFMSSQSSLSNERDYETVVMMRMRQAAERQRLIEQMQREDDEESAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUI0</accession>
    <name>A0A3B5LUI0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Tumor necrosis factor ligand superfamily member 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">TNF-related apoptosis-inducing ligand</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homotrimer. One TNFSF10 homotrimer interacts with three TNFSF10A mononers. One TNFSF10 homotrimer interacts with three TNFSF10B mononers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tumor necrosis factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUI0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011479"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011611.1">
        <property type="protein sequence ID" value="ENSXCOP00000011479.1"/>
        <property type="gene ID" value="ENSXCOG00000008665.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01130000278318"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001238">
        <property type="term" value="P:positive regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00184">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000014">
        <property type="entry name" value="Tumor necrosis factor ligand superfamily member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006052">
        <property type="entry name" value="TNF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017355">
        <property type="entry name" value="TNF_ligand_10/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11471:SF28">
        <property type="entry name" value="DEATH LIGAND 1B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11471">
        <property type="entry name" value="TUMOR NECROSIS FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00229">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038013">
        <property type="entry name" value="TNF10_TNF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00207">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50049">
        <property type="entry name" value="THD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0202">Cytokine</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="12" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11 19" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="THD" evidence="21">
        <location>
            <begin position="120"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between all trimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00055277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00074586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR038013-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="285" mass="31483" checksum="7CF95CE6DD037A51" modified="2018-12-05" version="1">MVPTGSPGGTARREAPSKMWLAMVVIVVVVLQIASTTGLFVYLNMSISQVRSQGVTEELKCLSLLNALDKDQDIPDQLAQLFGEPCIKLAEGIKAYISKARTMPRSFNNSKFMSSVAQRPSAHLTLRDTSSQGFSPYPIPTANLHQSCRHVVRSWANQSFGAHLHNMTLTNGRLRVPQDGRYYLYSQVYFRYPSPAASDGDQRSVSHQLVQCVYKKTSYPSPIQLLKGVGTKCWAPDAEYALHSVYQGGLFELRAGDELFVSVSSPTMVNADDSSSYFGAFRLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LW71</accession>
    <name>A0A3B5LW71_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Perforin 1.2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complement C6/C7/C8/C9 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LW71"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015574.1">
        <property type="protein sequence ID" value="ENSXCOP00000015382.1"/>
        <property type="gene ID" value="ENSXCOG00000011638.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063725"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022829">
        <property type="term" value="F:wide pore channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001771">
        <property type="term" value="P:immunological synapse formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001913">
        <property type="term" value="P:T cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020863">
        <property type="entry name" value="MACPF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052784">
        <property type="entry name" value="Perforin-1_pore-forming"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096:SF5">
        <property type="entry name" value="PERFORIN 1.2 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46096">
        <property type="entry name" value="PERFORIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00279">
        <property type="entry name" value="MACPF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017385153" description="Perforin 1.2" evidence="8">
        <location>
            <begin position="26"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="10">
        <location>
            <begin position="32"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="9">
        <location>
            <begin position="397"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="577" mass="64491" checksum="B24D8734F7A4F7DA" modified="2018-12-05" version="1">SSLQLMANLWQLMLLFWMWIPPGLSSTSFIGSPQQCETAHFVPGYNLGGEGFDIVTMERKGAYVIDTETWNLRNGTCKMYRNSYQNGENQKVPVSVADWRNLPKCSMKVSGMLYDSVESLVNDSTSSVTNDWKIDLNIPVDPTVTVGVGLGGSHSRASEFGMKKSKSDRYEFVRQSVSCNFYRYRLTTSPPLSRDFLAAVNSLPPYRPGKASPYLDLIDTYGTHYITQVYLGGEIKATTSFQTCKATMNGFTSTDVSDCLTVEASANFASSASVKAMTKHCEAKKKTLRSGQSFSSEFNERFTEVTGGDEVGVVLFGGGLDPDVYKNWLNSLKTIPDIVQYNLKPLHTILPENHRAKNGLKKEVEQYIFKNAVLKKCSETCQIGHRSNQRDPCACVCNSNKNVKANCCPAGKGLAILKVYKLYARGLYGDNWGKTDGSVEVKYGDQLKRTKIISNDDNPKWPEKFDFGQIVINMQNQLTFSVYDEDTHWNSDLLGWCSFELRKGLVTDSCMFNHGTFFFTYSVECAPSLGGSRCDEYISSPMNPSLAKEFYTRNGVLLGDTMKKFSKLARRASLDQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWD8</accession>
    <name>A0A3B5LWD8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphatidylinositol-4-phosphate 5-kinase, type I, gamma a</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWD8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012154"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012294.1">
        <property type="protein sequence ID" value="ENSXCOP00000012154.1"/>
        <property type="gene ID" value="ENSXCOG00000009195.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159258"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17308">
        <property type="entry name" value="PIPKc_PIP5K1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000001">
        <property type="entry name" value="phosphatidylinositol 4-phosphate 5-kinase type-1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF26">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-PHOSPHATE 5-KINASE TYPE-1 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="5">
        <location>
            <begin position="50"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="19"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="526"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="561"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="587" mass="65993" checksum="DC9FB824FDDC00C8" modified="2018-12-05" version="1">PHNGVGRFISSHVLLQRIPMPSSSGQPGQGKKIGHRGVDASGETTYKKTTSSALKGAIQLGIGYTVGNLSSKPERDVLMQDFYVVESIFFPSEGSNLTPAHHFPDFRFKTYAPVAFRYFRELFGIRPDDYLYSLCNEPLIELSNPGASGSVFYLTKDDEFIIKTVMHKEAEFLQKLLPGYYMNLNQNPRTLLPKFFGLYCVQSGGKNIRVVVMNNVLPRVVRMHLKYDLKGSTYKRRASKKEREKARPTFKDLDFMQDMPDGLMLDQDTYNAMVKTLQRDCLVLESFKIMDYSLLLGVHNMDQAERERQLEGSQGGSDEKRPVAQQKALYSTAMESIQGGAACGGSIDTDDTMGGIPAVNGKGERLLLYIGIIDILQSYRLIKKLEHTWKALVHDGDTVSVHRPGFYADRFFKFMSSTVFKKSSCDKGNGFKVIFTKLERFGAACFHSIKRFWVWWVERCREGVNACVFTRVLLVSRCRGVPACVFLFACFISCVVLRAVRTQEELRVREEDQQTITVEVELKRHDSEPTISVPQHSPEPAEEAPPRPEVVVVEPDGASLMSGSGCTSQASVDDDDDMPVSDIYFVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWS3</accession>
    <name>A0A3B5LWS3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Solute carrier family 41 member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a magnesium transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SLC41A transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWS3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012284"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012430.1">
        <property type="protein sequence ID" value="ENSXCOP00000012284.1"/>
        <property type="gene ID" value="ENSXCOG00000009288.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022890">
        <property type="term" value="F:inorganic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045349">
        <property type="entry name" value="SLC41A1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228">
        <property type="entry name" value="DIVALENT CATION TRANSPORTER SOLUTE CARRIER FAMILY 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228:SF23">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 41 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="34"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="43"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU369007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="105" mass="11273" checksum="DED6583B2D591D9F" modified="2018-12-05" version="1">MVTSFTERIGETGENEERSEIVVALDCRANAKGQREEDALLENASQSNESDDNSINQSPEPPVPLKETSISIGLQVVFPFLLAGFGTVAAGMLLDIVQVSYTATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWT9</accession>
    <name>A0A3B5LWT9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JIP scaffold family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWT9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013796"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013965.1">
        <property type="protein sequence ID" value="ENSXCOP00000013796.1"/>
        <property type="gene ID" value="ENSXCOG00000010446.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157089"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008432">
        <property type="term" value="F:JUN kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007254">
        <property type="term" value="P:JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046328">
        <property type="term" value="P:regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01212">
        <property type="entry name" value="PTB_JIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11943">
        <property type="entry name" value="SH3_JIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000108">
        <property type="entry name" value="C-Jun-amino-terminal kinase-interacting protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000032">
        <property type="entry name" value="Putative C-Jun-amino-terminal kinase-interacting protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047178">
        <property type="entry name" value="JIP1_scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035638">
        <property type="entry name" value="JIP1_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47437:SF3">
        <property type="entry name" value="C-JUN-AMINO-TERMINAL KINASE-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47437">
        <property type="entry name" value="JNK-INTERACTING PROTEIN 1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="9">
        <location>
            <begin position="243"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="PID" evidence="8">
        <location>
            <begin position="320"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="28"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="71"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="460" mass="51896" checksum="08E28CB39434314D" modified="2018-12-05" version="1">MDIGGSSPGLADWGGGPPVQKGGPEGVSGHREKIRHQADVHLEPTEEIYLTPVQRSADPLEPPNVQERPFLSQQTEQGRMSISSDTEGPPPYQPLPDRTNPSIFEEDELYVPPPSYASYGGGSKPAILRTTMSSEASSLTYDSVKYTLVVDEHAQLELVSLRQCYQGYSDDSDSATVYDNCVSSPYESAIGEEEATACLSEDSTPEEDLHFSKKFLNVFMNGRSRSSSAESFGLYSCIINGEEKDQTHRAVYRFVPRHDDELELEVDDPLLVEVQGEDYWYEGYNMRTGAHGIFPVYYAIEVNKDTDCYKVKSSEWMDRYRLKFLGSVQVPYHKGNDVLCAAMQKIANNRRMTVKFNPPSFCVLEISVKGIKLAVQEDYYSNECSHFFQLKNVSFCGYHPKNSKYFGFITKHPADQRFACHVFVSENSTKPLAESIGKAFQLYYKEFVEFSCPTEDIYLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXJ8</accession>
    <name>A0A3B5LXJ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Glypican-1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Cell surface proteoglycan.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Lipid-anchor</topology>
            <topology evidence="1 12">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glypican family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXJ8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016638"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016853.1">
        <property type="protein sequence ID" value="ENSXCOP00000016638.1"/>
        <property type="gene ID" value="ENSXCOG00000012553.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244897"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905475">
        <property type="term" value="P:regulation of protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001863">
        <property type="entry name" value="Glypican"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10822">
        <property type="entry name" value="GLYPICAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10822:SF25">
        <property type="entry name" value="GLYPICAN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01153">
        <property type="entry name" value="Glypican"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 12" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9 12" id="KW-0357">Heparan sulfate</keyword>
    <keyword evidence="10 12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017217273" description="Glypican-1" evidence="13">
        <location>
            <begin position="23"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16010" checksum="7F8C08481346A55F" modified="2018-12-05" version="1">MKPLSVLCAVCTLAVLSASATAEPKLKNCNEVREAFTSKGFNMNDAPNKVVNGAPLKVCPQGFTCCTAEMEDKLSQQSHTEIKAPVSRLSTSLQSTFKQRHDHFDSKQTLLHKHTHTHTHTADKVVQTWHSLESLPVWGWAIWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZC9</accession>
    <name>A0A3B5LZC9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Potassium channel tetramerization domain containing 14</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027900359.1">
        <property type="nucleotide sequence ID" value="XM_028044558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZC9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016522"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016732.1">
        <property type="protein sequence ID" value="ENSXCOP00000016522.1"/>
        <property type="gene ID" value="ENSXCOG00000012474.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114161337"/>
    <dbReference type="KEGG" id="xco:114161337"/>
    <dbReference type="CTD" id="65987"/>
    <dbReference type="GeneTree" id="ENSGT00940000160762"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057890">
        <property type="entry name" value="KCTD7/14_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499:SF3">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN KCTD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14499">
        <property type="entry name" value="POTASSIUM CHANNEL TETRAMERIZATION DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25611">
        <property type="entry name" value="KCTD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BTB" evidence="1">
        <location>
            <begin position="21"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="241" mass="27411" checksum="9A84A10A39E72DE6" modified="2018-12-05" version="1">MSLPDYKSTGRQPSSVLSRSPVVQLNVGGHLFSTTLSTLRRHPDSKVAELFGSQPKVSTDAQGRFFIDRDGSHFGAVLEFLRSEKLPTENIQEVYTEAVHYNIKELIKRLEETPELFGELVGRQQFLSRVPHYKENIEVLIRIARAEAVAARRSTILICVMRTEDDLGFYDNAVNSLEAGTESAVTFGPWKADPSVRDLLDCVKMDIARRGYNISIEAHAPERSFLSKSYDFFYKLTFTWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZJ1</accession>
    <name>A0A3B5LZJ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP cyclohydrolase 1 feedback regulatory protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">GTP cyclohydrolase I feedback regulatory protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GFRP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027857491.1">
        <property type="nucleotide sequence ID" value="XM_028001690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZJ1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014776"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014962.1">
        <property type="protein sequence ID" value="ENSXCOP00000014776.1"/>
        <property type="gene ID" value="ENSXCOG00000011204.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114134837"/>
    <dbReference type="KEGG" id="xco:114134837"/>
    <dbReference type="CTD" id="2644"/>
    <dbReference type="GeneTree" id="ENSGT00440000033849"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044549">
        <property type="term" value="F:GTP cyclohydrolase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009890">
        <property type="term" value="P:negative regulation of biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1410.10:FF:000001">
        <property type="entry name" value="GTP cyclohydrolase 1 feedback regulatory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1410.10">
        <property type="entry name" value="GTP cyclohydrolase I feedback regulatory protein GFRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036717">
        <property type="entry name" value="GFRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009112">
        <property type="entry name" value="GTP_CycHdrlase_I_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16852">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1 FEEDBACK REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16852:SF2">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1 FEEDBACK REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06399">
        <property type="entry name" value="GFRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69761">
        <property type="entry name" value="GTP cyclohydrolase I feedback regulatory protein, GFRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="86" mass="9723" checksum="6F56A99357762372" modified="2018-12-05" version="1">MPYIFISTQIRLESGPTNVGDEFSDPSIMSYLGARKTTMLGNNFSEYHVDDPPRLVLDKLEKIGFRLLSMTGVGQTLVWCLHKESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZQ0</accession>
    <name>A0A3B5LZQ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cell morphogenesis protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZQ0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013229"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013392.1">
        <property type="protein sequence ID" value="ENSXCOP00000013229.1"/>
        <property type="gene ID" value="ENSXCOG00000010020.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00610000086058"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025614">
        <property type="entry name" value="Cell_morpho_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039867">
        <property type="entry name" value="Furry/Tao3/Mor2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295">
        <property type="entry name" value="FURRY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295:SF29">
        <property type="entry name" value="PROTEIN FURRY HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14222">
        <property type="entry name" value="MOR2-PAG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cell morphogenesis protein N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="426" mass="48331" checksum="D87B39668A489CDD" modified="2018-12-05" version="1">FLSRWHIFLNNCLSNLKNRDPKMARVALESLYRLLWVYMIRIKCESNTATQSRLTSITSTLFPKGSRSVVPRDMPLNIFVKIIQFIAQERLDFAMKEIIFDLLSVGKPAKAFSLNPERMNIGLRAFLVIADALQQKDGEPPMPNTGATLPSGNSLKKKKTYLSKTLTEEEAKLIGMSLYYSQVRKALDNILRHLDKEVGRCMMLTSVQMLNKEPEDMITGERKPKIDLFRTCVAAIPRILPDSMSKPELIDLLSRLTVHMDDELRLISQNSLQSLLLDFSDWREDVLFGYTHFLLREVQDTHQGLQDASVKLLLQLLTQWRLALQLQGKMRGGTDVRNNKSHPRLPERSPHCSVLHAVEGLALLLLCSCQISTRKLAVGVLREIRCLFTALGHDDDKPMIEILDQLSPAVMNSFVHVAHSDSVSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZR8</accession>
    <name>A0A3B5LZR8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Mitogen-activated protein kinase kinase kinase 21</fullName>
            <ecNumber evidence="3">2.7.11.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Mitogen-activated protein kinase kinase kinase MLK4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Mixed lineage kinase 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Negative regulator of TLR4 signaling. Does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer. Interacts with TLR4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZR8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016480"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016687.1">
        <property type="protein sequence ID" value="ENSXCOP00000016480.1"/>
        <property type="gene ID" value="ENSXCOG00000012440.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159629"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004706">
        <property type="term" value="F:JUN kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12059">
        <property type="entry name" value="SH3_MLK1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14146">
        <property type="entry name" value="STKc_MLK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000076">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000169">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000085">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035779">
        <property type="entry name" value="MLK1-3_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF30">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 19" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SH3" evidence="23">
        <location>
            <begin position="42"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="135"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="552"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="648"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="725"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="421"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="22">
        <location>
            <begin position="567"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="588"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="653"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="766"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00053407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00076612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00078274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="868" mass="96107" checksum="4EAD3417AFDDFB3A" modified="2018-12-05" version="1">MDVSQAASPNGEGRPGGGGAGEHAWTECPTVRSWAHSAPLCPTRSLWTAAYDYEASGEDELSLRRGDVVEVLSKDAAISGDEGWWTGKLNHRVGIFPSNYVTYQPAIYRLPATSGSAGVSEQVPSAPTQIPFSELVLEEIIGVGGFGKVYRGTWKDQEVAVKAARQDPDEDINATAASVKQEAKLFSMLQHPNIIKLEGVCMEEPNLCLVMEYARGGTLNRALTGRRIPPHILVNWAVQIARGMLYLHEDAVVRIIHRDLKSSNILLLEKIENDDIGRKTLKITDFGLAREWHKTTKMSAAGTYSWMAPEVIKSSLFSKGSDVWSYGVLLWELLTGEVPYRGIDGLAVAYGVAVNKLTLPIPSTCPEPFAKLMEECWDQDPHVRPSFSCILEQLSAIEEAVMATMPQDSFHSMQDDWRVEIQEMFDELRTKEKELRSREEELTRAALQQKSQEELLKRREQQLAEREINVLERELNILIFQLNKDKPNVKKRKGKFKRSRLKLKDGNRISLPSGEKQMYNKPLRLYLQKEKETNLFLVLITFDQSCVCFQREQAGRKRSPRNPQSPGGNGGPGDAGVGAGSPPETPEDPCTTTSTSSTSTTPQLTPTNSLKRASARRKTDFALYGCGSLLASVVLGYDIREAIRTSAPPEECETPKEEKKKKEGLFQRATRFRRSTSPPGGRSRKEDTSSNSAGAPPVNLVSMLAIMECNSTKCLLQPEAEVSELSPGKAEPAVSDHNSETSRPVRAATVKSQTHNGPPAPSAAQRKQEKEEKPANGDAVPRQRPVSFRAKPHAWALLRGRNKSYSLGHYSGEKTAQDLNVVLSSEVGCSLLDMDTEGQKRDCTVPLCRIQSSPARPSMFELEKEFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0A9</accession>
    <name>A0A3B5M0A9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinase</fullName>
            <ecNumber evidence="4">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0A9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016695"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016910.1">
        <property type="protein sequence ID" value="ENSXCOP00000016695.1"/>
        <property type="gene ID" value="ENSXCOG00000012591.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160033"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000828">
        <property type="term" value="F:inositol hexakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF106">
        <property type="entry name" value="INOSITOL-TRISPHOSPHATE 3-KINASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="256" mass="29453" checksum="678FCAA9022F6AF2" modified="2018-12-05" version="1">DIESSLSPTRSPCSAGDGSGLQSKSWSKLKSMVHFSPLAFTLKPRHSWVQLAGHTSFTAGDGGRLLKRYCECEQRCLEKLMKDVLRPYVPGYYGVLHKDEQDFNIMDDLLTDFDMPSIMDCKMGSRTCLEEELIKAKECQRLRRDMYEKMISVDPAAPTEEERAQQGVLKPRYMRWRETLSSTATLGFRIEGIKQKGDGSCNTGFKKTKHREQVVKAFEDFVDGDKSYLKRLEELRSVLEKSDFFRTHEVRTRFPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0Q2</accession>
    <name>A0A3B5M0Q2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0Q2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015191"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015381.1">
        <property type="protein sequence ID" value="ENSXCOP00000015191.1"/>
        <property type="gene ID" value="ENSXCOG00000011507.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157764"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000038">
        <property type="entry name" value="Putative sodium-coupled neutral amino acid transporter 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF226">
        <property type="entry name" value="SODIUM-COUPLED NEUTRAL AMINO ACID TRANSPORTER 8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="250"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="337"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="360"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="403"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="9">
        <location>
            <begin position="28"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="433" mass="47830" checksum="23326E6ED73DF496" modified="2018-12-05" version="1">MEELARESISLLARSASLADPPRLGSFGAVFIMLKSALGAGLLNFPWAFGKAGGVNTAITVELVSLVFLISGLVILGYASSISRQKTYQDVVREVCGRAVGQLCEVCFCFNLFMISVAFLVVVQDQLEKLCISLYETVTGSSEADMPYHWYTDQRFALFIMCLLIILPLSIPKEIGIQKYTSVLGTLAATYLCVAVVVKHYLMDSHRIIYPEQSRGTWTSMFSVVPTICFGFQCHEACIAIYSSMENQKLLHWAAISVLSMFFCLLTYTLTGVYGFLTFGQGVASDILMSYPGNDVVMIISRLLFGVSIITIYPIILLLGRNRRGVVTHSFESRCRVVLTMAWISFTLLIAMFVPDMADVISVIGGISAFFIFIFPGLCLVCSPRLSSDLLSFVCHRRDILTIWGVVTIVVGAFIFGQSTTIAVMEIFHKFKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M1D5</accession>
    <name>A0A3B5M1D5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kazrin, periplakin interacting protein b</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M1D5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017898"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018122.1">
        <property type="protein sequence ID" value="ENSXCOP00000017898.1"/>
        <property type="gene ID" value="ENSXCOG00000013483.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154570"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037614">
        <property type="entry name" value="Kazrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059089">
        <property type="entry name" value="Kazrin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12776:SF2">
        <property type="entry name" value="KAZRIN ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12776">
        <property type="entry name" value="KAZRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25986">
        <property type="entry name" value="Kazrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="3">
        <location>
            <begin position="408"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="3">
        <location>
            <begin position="492"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="289"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="710"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="64"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="761" mass="86974" checksum="1C276F7375E25675" modified="2018-12-05" version="1">MMEDNKQLAQRIDGAIQSASQEVTNLRSELSATSRKLAELGASSPLATISTTLLNKTTLLREEVVQLQEEVHLLRQMKDMLSKDLEETQGGCSANLLSATELRAQLGDKEQELDRAKEALQAMKADRKRLKVEKSELANQMQQLYTTLESREEQLREFIRNYDQHRKESEDAVKVLAKEKDVLEREKWDLRRQTKESTEQANMLRSQIDMKENRIKELEAELTMAKQSLATLTKDVPKRHSLVVQPEPLVNGSQEWVMQADLPLTAAIRQSQQTLYHGHNADRQAVVRISPCHTRQPSVISDASAADGDRSSTPSDINSPRHRTHSLCNVRNAPVSMEDLEDQKRKKKKDKMTLGSLSRVFTRGKQRKSLDPGLFDDSDTQHSLSDGEEQLDRLQQAELSRTTCMSHWRAGAVQAWMEVVMGMPMYIRACSENVKSGKVLLSLTDEDLELGLGIGNQIHRRKVRLAIEDYRRAEGELSKASELDHHWVSTSWLSDVGLPQYSQTFQTNLVDGRVLNSLSRRDLERFLNIGDHFHQTSLLLAIQLLQMLSFDKEALQLRRLKCEHENRDPLVWTCHRVMKWIKDIDLKEFAENLQGKGIHGAVIALDQSFDTEALANALGIPSNKHMLHRHLYEEMKSLSREAFFSDRWPAVSSNRLGKDCVESGGNTLHPQINSLISCVVFSESAEVTRRKSLRGARPWFPWQLRLSAQRGQSSRCSPRQRKPHAHLQERRNHQRVKEPWRCSLFTLLCCQLYNVGAFLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2B1</accession>
    <name>A0A3B5M2B1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NUC153 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M2B1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014159"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014336.1">
        <property type="protein sequence ID" value="ENSXCOP00000014159.1"/>
        <property type="gene ID" value="ENSXCOG00000010728.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004881"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039754">
        <property type="entry name" value="Esf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012580">
        <property type="entry name" value="NUC153"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202:SF0">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08159">
        <property type="entry name" value="NUC153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NUC153" evidence="4">
        <location>
            <begin position="55"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="73"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="106" mass="12221" checksum="D2DB32996EA2CADB" modified="2018-12-05" version="1">MALLMEDDEDEAKHKHFNYDKIVEQQNLSRRKRKKLLRKGEAPLEDDGFQVDVSDPRFQAVFTSHLFNLDPSHPGFRNTRATRNILEEKQRQPPGPAPLKGSAPDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2H4</accession>
    <name>A0A3B5M2H4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M2H4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018529.1">
        <property type="protein sequence ID" value="ENSXCOP00000018298.1"/>
        <property type="gene ID" value="ENSXCOG00000013780.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="90" mass="9891" checksum="065BDB8585D9F8FF" modified="2025-10-08" version="2">MTATLVNYVQLRGVVTLNRGSGAAGGAAPRERMCGTATRNHSLYICRTGRLQFKCVFMLTIVFSIIFLETVIVLVNDFLPNVLLIILGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2N4</accession>
    <name>A0A3B5M2N4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Small integral membrane protein 19</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMIM19 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M2N4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017901.1">
        <property type="protein sequence ID" value="ENSXCOP00000017677.1"/>
        <property type="gene ID" value="ENSXCOG00000013327.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000436"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029368">
        <property type="entry name" value="SMIM19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31888">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31888:SF1">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15117">
        <property type="entry name" value="UPF0697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="116" mass="13747" checksum="EE32E9E8522F0624" modified="2018-12-05" version="1">MLHKNSSIIFLFFNIFEVAVCNFLNGFYIFVKTVCDVTVWYETDNVKKIKVRPPSRKIMRIFTLPPTVGSSPEPNFYDSLQKVRLRQQLEMYSLARKFEQQQQQQQQTDSVQLSME</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M796</accession>
    <name>A0A3B5M796_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DnaJ heat shock protein family (Hsp40) member B1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027873508.1">
        <property type="nucleotide sequence ID" value="XM_028017707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M796"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019350"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019588.1">
        <property type="protein sequence ID" value="ENSXCOP00000019350.1"/>
        <property type="gene ID" value="ENSXCOG00000014547.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114144647"/>
    <dbReference type="KEGG" id="xco:114144647"/>
    <dbReference type="CTD" id="327244"/>
    <dbReference type="GeneTree" id="ENSGT00940000164566"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000005">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily B, member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000002">
        <property type="entry name" value="Dnaj homolog subfamily b member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000007">
        <property type="entry name" value="dnaJ homolog subfamily B member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051339">
        <property type="entry name" value="DnaJ_subfamily_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078:SF568">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="4"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="354" mass="38773" checksum="8ECE4968904DFA55" modified="2018-12-05" version="1">MGKDYYDILGIKKGASEDDIKKAYRKQALRYHPDKNKSPGAEEKFKEIAEAYDVLSDPKKKDIYDRFGEEGLKGGGGGGGPTGGSGPGTYSYTFHGDPHAIFAEFFGGRNPFEQFFGARNGGMDENMDTDDPFAQFGMAGRGMGPGGMGGFSHSFNPGMGGMGGHTSVVKKQQDPPVVHDLNVSLEEVLQGCTKKMKISRKRLSPDGRTTRTEEKILEVQIKKGWKEGTKITFAKEGDETPRNIPADVVFVLKDKPHPVFKRDGSDVIYTAKVSLRDALCGCTVQAPTLDGRTVTVTSTDVVQPGMKRRVSGEGLPYPKRPDRRGDLIVEYEVKFPERLSKNARDTIAQVLPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7I9</accession>
    <name>A0A3B5M7I9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the eukaryotic/archaeal RNase P protein component 4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027882608.1">
        <property type="nucleotide sequence ID" value="XM_028026807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7I9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017296"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017517.1">
        <property type="protein sequence ID" value="ENSXCOP00000017296.1"/>
        <property type="gene ID" value="ENSXCOG00000013033.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114150422"/>
    <dbReference type="KEGG" id="xco:114150422"/>
    <dbReference type="CTD" id="79897"/>
    <dbReference type="GeneTree" id="ENSGT00390000003020"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04032">
        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="114"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="142"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="157" mass="17816" checksum="6B661A8BFD6D007F" modified="2018-12-05" version="1">MAVHLKDREAYERLNYLYQAAHCVLSQNPNNVELARFYCFTQKTIARRLVLRQDPSVKRTVCKKCCSLLIPGVTATTRQRRKNKKNRFTVVRCLGCGQTKNLLNNPDHCLWADRPEAQLEQRKQSDPAPSAKNQPKCPKADGSQSSKTHDAQSSTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7M0</accession>
    <name>A0A3B5M7M0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7M0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015784"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015979.1">
        <property type="protein sequence ID" value="ENSXCOP00000015784.1"/>
        <property type="gene ID" value="ENSXCOG00000011926.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164226"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042923">
        <property type="term" value="F:neuropeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004983">
        <property type="term" value="F:neuropeptide Y receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15397">
        <property type="entry name" value="7tmA_NPY4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000062">
        <property type="entry name" value="Neuropeptide Y receptor type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000351">
        <property type="entry name" value="NPY1_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000611">
        <property type="entry name" value="NPY_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235">
        <property type="entry name" value="NEUROPEPTIDE Y RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24235:SF25">
        <property type="entry name" value="NEUROPEPTIDE Y RECEPTOR TYPE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01013">
        <property type="entry name" value="NRPEPTIDEY1R"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01012">
        <property type="entry name" value="NRPEPTIDEYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 15" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11 15" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 15" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 15 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="65"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="45"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="386" mass="44641" checksum="A5C6F7D5B8649609" modified="2018-12-05" version="1">LNSENLSPAPYLSVLEQEEQCHMSPVLTTCLVVWYSVTMVLGLVGNIGLICIITHRREKVNVTSIFICNLSFSDILVCMFCLPFTVIYTLMDHWVFGSLMCRLVPFIQCVSVTVSVLSLVFIALERHQLIINPSGWKPSIPQAYMAVIFIWILASFTSTPFLAFQLLTNEPYTNVVLPLHPQLYPPVYLNHWPSHEHRVAYTTWLLLFQYCGPLLLVLLCYVRVFVRLRHRKDMLDRARTSENQRMTNSRRINIMLVALITAFALCWLPLTIFNVLSDWNLEALPVCHHNLLFSLCHLLAMSSTCINPIIYGFLNSNFRQEVQEVLLHCRCFPQEEEFEHFPMSTVNMEMSRTSVPLQSLPTLFKSLRDDNEIAMKFLQIWSFTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9A3</accession>
    <name>A0A3B5M9A3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome P450, family 27, subfamily A, polypeptide 7</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9A3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020100"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020347.1">
        <property type="protein sequence ID" value="ENSXCOP00000020100.1"/>
        <property type="gene ID" value="ENSXCOG00000015067.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182905"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006700">
        <property type="term" value="P:C21-steroid hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071375">
        <property type="term" value="P:cellular response to peptide hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034650">
        <property type="term" value="P:cortisol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006704">
        <property type="term" value="P:glucocorticoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042359">
        <property type="term" value="P:vitamin D metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000006">
        <property type="entry name" value="Cytochrome P450 302a1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF123">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 27 SUBFAMILY A MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="503" mass="57730" checksum="511E404CAC3495F6" modified="2018-12-05" version="1">MAAWLSRSAACGICSLRRPLCGGALRWSSSALQEKLRSVQDLPHVSALEMLYRVLVQGYFSSLHMLQVYEKQLYGPIYKNGRNAVSVSSAELLEELVRKDDKFPTRGDMDLWTEYREMRGLGYGPLTEEGERWYKLRAVLNKRMLHPRDSAQYDGVINAVVTDFIKRIKRLRLCSPTGDLVPDIANKFYLFSLEGISSILFEARLGCLSEEIPAGTQEFITSISQMFSNAIYVAIFPKWSRSLLPYWQRYIASWDGIFSFGKTLIDKKMEDIQQRVDNDQDVEDEYLTYLLSNSQMSTRDIYASVTELLLAGVDTTSNTLTWALYQLSRNPETQNRLYEEVSSLVPADRIPSAAEVTAMPFLRAVIKETLRMYPVVPINSRIIKEKPVAVGGYQFPEKTGFTLCHYAISHDENIFPEPYRFKPERWLRDGRQRPNAFGSIPFGYGVRGCVGRRIAELEMYLVLFRLIREFEIKPNPTADDLKIVSRAVLVPNKQLDLHFVDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9M7</accession>
    <name>A0A3B5M9M7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">T cell leukemia homeobox 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9M7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020468.1">
        <property type="protein sequence ID" value="ENSXCOP00000020220.1"/>
        <property type="gene ID" value="ENSXCOG00000015040.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165256"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048839">
        <property type="term" value="P:inner ear development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000040">
        <property type="entry name" value="T-cell leukemia homeobox protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042247">
        <property type="entry name" value="TLX1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45921">
        <property type="entry name" value="IP01054P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45921:SF3">
        <property type="entry name" value="T-CELL LEUKEMIA HOMEOBOX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="178"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="180"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="307" mass="34412" checksum="ABE02483FADC0FA2" modified="2018-12-05" version="1">FLRARSSSASRVNQTHQQQHEPISFGIDQILNSSDQSSGCMLPNRTGDPDYALAPNVYSNGYNSVYNPACSYNVNMNMNVSMNMNMNVNVNSGNSGGVIRVPAHRPMPPPPPPSAAAAPHPPPGIPAVPGMGMGNPANFTFPWMESSRRFAKDRLTAALSPFSVTRRIGHPYQNRTPPKRKKPRTSFSRVQICELEKRFHRQKYLASAERATLAKALKMTDAQVKTWFQNRRTKWRRQTAEEREAERQQANRLMLQLQQEAFQKTLSQPLQPDPLCLHNSSLYALQNLQPWAEDNKVTSVTSVASVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9Y7</accession>
    <name>A0A3B5M9Y7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bone Gla protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Gamma-carboxyglutamic acid-containing protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">The carboxylated form is one of the main organic components of the bone matrix, which constitutes 1-2% of the total bone protein. The carboxylated form binds strongly to apatite and calcium.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the osteocalcin/matrix Gla protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027872129.1">
        <property type="nucleotide sequence ID" value="XM_028016328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9Y7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018091"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018322.1">
        <property type="protein sequence ID" value="ENSXCOP00000018091.1"/>
        <property type="gene ID" value="ENSXCOG00000013639.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114143922"/>
    <dbReference type="KEGG" id="xco:114143922"/>
    <dbReference type="CTD" id="337724"/>
    <dbReference type="GeneTree" id="ENSGT00410000026290"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046848">
        <property type="term" value="F:hydroxyapatite binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008147">
        <property type="term" value="F:structural constituent of bone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031214">
        <property type="term" value="P:biomineral tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060348">
        <property type="term" value="P:bone development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001649">
        <property type="term" value="P:osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900076">
        <property type="term" value="P:regulation of cellular response to insulin stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032571">
        <property type="term" value="P:response to vitamin K"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058704">
        <property type="entry name" value="BGLAP-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035972">
        <property type="entry name" value="GLA-like_dom_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000294">
        <property type="entry name" value="GLA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039176">
        <property type="entry name" value="Osteocalcin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14235">
        <property type="entry name" value="OSTEOCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14235:SF0">
        <property type="entry name" value="OSTEOCALCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25890">
        <property type="entry name" value="BGLAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00069">
        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57630">
        <property type="entry name" value="GLA-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00011">
        <property type="entry name" value="GLA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50998">
        <property type="entry name" value="GLA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0091">Biomineralization</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0301">Gamma-carboxyglutamic acid</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017201595" description="Bone Gla protein" evidence="13">
        <location>
            <begin position="19"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Gla" evidence="14">
        <location>
            <begin position="137"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="33"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="33"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="187" mass="18757" checksum="BF342EDA49823506" modified="2018-12-05" version="1">MKTLTLLSICALLSVCWSMGAVEPEVVVDPAADTAADPAADAPASSSSSSSESDSASSSASDSSASDSTSDSSSSSESSESSESSSAESSESSSSESNSSASDSAASSSSSSSSSSESAGAEESHVVMKRDLASVLLRRRRAAQGNLSPLQLESLKEVCELNVACDDMADTEGIVAAYTAYYGPVPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MA80</accession>
    <name>A0A3B5MA80_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">P-type ATPase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MA80"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021163"/>
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        <property type="protein sequence ID" value="ENSXCOP00000021163.1"/>
        <property type="gene ID" value="ENSXCOG00000015835.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
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        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF38">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="2">
        <location>
            <begin position="4"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="137" mass="15791" checksum="D7E3038D73B81473" modified="2018-12-05" version="1">IPPKFCDNRIVSSKYTVWNFLPKNLFEQFRRIANFYFLIIFLVQVMDTPTSPVTSGLPLFFVITVTAIKQVRPCLMRQISNVHYFVPGLVYLLDLLFSSSFGQLTSTSHVTGIVLLSFSKVIIVFMPHFFPHSVTFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MA93</accession>
    <name>A0A3B5MA93_9TELE</name>
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        <recommendedName>
            <fullName evidence="2">Glyoxylate reductase/hydroxypyruvate reductase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027874320.1">
        <property type="nucleotide sequence ID" value="XM_028018519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MA93"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018201"/>
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        <property type="gene ID" value="ENSXCOG00000013708.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="xco:114145121"/>
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    <dbReference type="GeneTree" id="ENSGT00940000165381"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008465">
        <property type="term" value="F:hydroxypyruvate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05301">
        <property type="entry name" value="GDH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glyoxylate/hydroxypyruvate reductase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
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        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF230">
        <property type="entry name" value="GLYOXYLATE REDUCTASE_HYDROXYPYRUVATE REDUCTASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="4">
        <location>
            <begin position="39"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="147"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00073306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="359" mass="39139" checksum="772EC30B7C1177D5" modified="2018-12-05" version="1">MLSSRMALRRLQRIAVAPLSFTSGATTKVQRDMSTLPRVYVTRQIPSEGLKILQDSGQVQFELWDSDDVPVPRKELLQKVKGVDGLLCVLTEKIDAELLDAAGSNLKVVSTMSVGFDHLSLEELKKRGIRVGYTPEVLTDAVAELTVALLLATSRRLIEATHEAKTGGWGTWRTLWLCGYELANSTVGILGLGRIGVAIAERLAPFKVKKFIYTDVAPRPELAKAINAEYVTLDELAKQSDFLSVCCALTPETKEICNTNLFSKMKNTSIFINTSRGGVVNQEDLYEALSTGQIAGAGLDVTVPEPLPTSHPLFTLKNCVILPHIASASYSTRNAMSALAANNLLLGLRSQPMIKELKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MB89</accession>
    <name>A0A3B5MB89_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">E3 ubiquitin-protein ligase TRIM21-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSXCOP00000021513.1"/>
        <property type="gene ID" value="ENSXCOG00000016109.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000240400"/>
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        <property type="component" value="Unplaced"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06503">
        <property type="entry name" value="ATP-synt_Fo_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19769">
        <property type="entry name" value="Bbox2_TRIM16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13733">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000004">
        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058030">
        <property type="entry name" value="TRIM8/14/16/25/29/45/65_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF32">
        <property type="entry name" value="BLOODTHIRSTY-RELATED GENE FAMILY, MEMBER 16 ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25600">
        <property type="entry name" value="TRIM_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="1" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="15"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="9">
        <location>
            <begin position="148"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="10">
        <location>
            <begin position="352"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="246"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="552" mass="62904" checksum="A59C320A8A816A95" modified="2018-12-05" version="1">MSSGSNLQSKDQFLCSICLDVFTDPVSTSCGHNFCKTCITQLWDGNVSYRCPLCNKHFASRPQLRVNTLLKEMVDQFRGEAQQETSSSSEQQAAKPGEVPCDVCTGPKLKALKSCMVCLLSYCQTHLEPHLTAPRLKKHQLMEPVENLEDRMCLQHDRPLELFCRTDQRCVCLLCPVLDHKNHEFVPLREESEGKKAELRKTEPEVQEMIQERRLKIQEIRESVKISKDAADREKAEGVQVFTALIESAERGLKELLKEIQDKQETTEKQAEDFIRDLEQEISELKKRSSEVKQLSQDEDHLHLLQRFSSLKDAPPTKTWTKVRVLPPSYDGTVVRAGTQLQEEMKRMMEAEMKRMKRMMEMKRIHQFAVDVTLDPDTASPWLILSDDGKQVKDGDVKKNLPDNPERFDQCVNVLGQQSFSSGKFYFEVQVKGKTEWDLGVARESINRKGKIAATPQDGFWTVVLRNGNEYKAAAGPSVRLHLHPGPQKVGVFVDYEEGLVSFYDVDAAALIYSFTGCCFKEKLYPYFSPCKNDLGKNSDPLIICPVNQADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MC12</accession>
    <name>A0A3B5MC12_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Beta-1,4-galactosyltransferase</fullName>
            <shortName evidence="17">Beta-1,4-GalTase</shortName>
            <ecNumber evidence="17">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N-acetyl-D-glucosamine + UDP-alpha-D-galactose = beta-D-galactosyl-(1-&gt;4)-N-acetyl-D-glucosamine + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17745"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:60152"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="ChEBI" id="CHEBI:506227"/>
            <dbReference type="EC" id="2.4.1.90"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:17746"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Golgi apparatus membrane</location>
            <topology evidence="2 17">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the glycosyltransferase 7 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MC12"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021080"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021339.1">
        <property type="protein sequence ID" value="ENSXCOP00000021080.1"/>
        <property type="gene ID" value="ENSXCOG00000015769.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155244"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003831">
        <property type="term" value="F:beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003945">
        <property type="term" value="F:N-acetyllactosamine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00899">
        <property type="entry name" value="b4GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000028">
        <property type="entry name" value="beta-1,4-galactosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003859">
        <property type="entry name" value="Galactosyl_T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027791">
        <property type="entry name" value="Galactosyl_T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027995">
        <property type="entry name" value="Galactosyl_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19300:SF5">
        <property type="entry name" value="BETA-1,4-GALACTOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02709">
        <property type="entry name" value="Glyco_transf_7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13733">
        <property type="entry name" value="Glyco_transf_7N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02050">
        <property type="entry name" value="B14GALTRFASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14 17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 17" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 17" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15 17" id="KW-0464">Manganese</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase N-terminal" evidence="19">
        <location>
            <begin position="95"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Galactosyltransferase C-terminal" evidence="18">
        <location>
            <begin position="233"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU368121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF13733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="354" mass="39915" checksum="C92FD727BD0F4350" modified="2018-12-05" version="1">MGTSVASDLSIREMKKKITIILALSVLFTLTCFTVFLLHSKDTSLVFPSLPQQVVKGSRSVFGKMMDHIRVKKIPGVSRETAATSAQPAPPLDPCPDSPPSLVGPVLVEFETIRSLQWVRNQLGSVLQQGGRYKPPDCTARQKVAVIIPFRNRYEHLTHWLYYLHPVLMRQQLDYGVYVINQDGDGAFNRAKLMNIGFVEALKEYDYDCFVFSDVDLVPIDDRNLYRCSDHPRHLSVAIDKFNYILSSVTAFGGVSLLTEEQFLKVNGFSNTFWGWGGEDDDLYNRIIVRGMSITRPDAGIAKYKMIKHGRDLHNEVNPENNIETDGLNSLNFTVKEILKDVLFTLISVDVQAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MC18</accession>
    <name>A0A3B5MC18_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">SRY-box transcription factor 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027864730.1">
        <property type="nucleotide sequence ID" value="XM_028008929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MC18"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021162"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021421.1">
        <property type="protein sequence ID" value="ENSXCOP00000021162.1"/>
        <property type="gene ID" value="ENSXCOG00000015821.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114139180"/>
    <dbReference type="KEGG" id="xco:114139180"/>
    <dbReference type="CTD" id="6658"/>
    <dbReference type="GeneTree" id="ENSGT00940000162359"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905040">
        <property type="term" value="P:otic placode development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043049">
        <property type="term" value="P:otic placode formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001541">
        <property type="term" value="P:ovarian follicle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007422">
        <property type="term" value="P:peripheral nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01388">
        <property type="entry name" value="HMG-box_SoxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000002">
        <property type="entry name" value="transcription factor Sox-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022097">
        <property type="entry name" value="SOX_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF111">
        <property type="entry name" value="TRANSCRIPTION FACTOR SOX-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12336">
        <property type="entry name" value="SOXp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="8">
        <location>
            <begin position="35"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="6">
        <location>
            <begin position="35"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="13"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="300" mass="33456" checksum="C087AD3C815ADC87" modified="2018-12-05" version="1">MYNMMETEIKTPLPQSNSGSAPGAKNNSASDQERVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGADWKLLTDAEKRPFIDEAKRLRAMHMKEHPDYKYRPRRKTKTLLKKDKYSLPGGLLAPGSNAVNNSVSVGQRMDGYAHMNGWTNSAYSLMQDQLAYPQHHSMNSPQIQQMHRYEMAGLQYPMMSSAQTYMNAASTYSMSPAYTQQTTSAMGLSSMASVCKTEPSSPPPAITSHSQRACLGDLRDMISMYLPPGGDSAEHSSLQSSRLHSVHPHYQTAGTGVNGTLPLTHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCZ7</accession>
    <name>A0A3B5MCZ7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCZ7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017797.1">
        <property type="protein sequence ID" value="ENSXCOP00000017574.1"/>
        <property type="gene ID" value="ENSXCOG00000013254.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000276573"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="54" mass="5790" checksum="E3156CEE85791241" modified="2018-12-05" version="1">MNLCGVPPSTAISFKCRSGRCSLSKAFCSTISTFLLPSVFTCCFSIKLSFGDKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFA4</accession>
    <name>A0A3B5MFA4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Si:dkey-57c15.1</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MFA4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022275"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022546.1">
        <property type="protein sequence ID" value="ENSXCOP00000022275.1"/>
        <property type="gene ID" value="ENSXCOG00000016641.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165535"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3590.10">
        <property type="entry name" value="acid-sensing ion channel 1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001873">
        <property type="entry name" value="ENaC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690:SF222">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11690">
        <property type="entry name" value="AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00858">
        <property type="entry name" value="ASC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01078">
        <property type="entry name" value="AMINACHANNEL"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 11" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="9 11" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="185" mass="21465" checksum="01FA5047D6B06AAA" modified="2018-12-05" version="1">MDLKADPEDVDYKRPAPIEVFATRSTLHGISHMFTYERKYVKRSLWIVFFLASLGVLVMVCVDRVQFYFEYPHVTKLDEVAASMIVFPAVTFCNLNSFRFSQVTHNDLYHVSPAAKEALLALLDFKNFLPPPLESQPSMKELLDRLGHQLEEMLLYCRYQGEPCGPRNFSIRSFSYLKQQPRSCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MG35</accession>
    <name>A0A3B5MG35_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">G protein-coupled receptor 137c</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MG35"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023278"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023556.1">
        <property type="protein sequence ID" value="ENSXCOP00000023278.1"/>
        <property type="gene ID" value="ENSXCOG00000017385.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153986"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21475">
        <property type="entry name" value="7tm_GPR137C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029723">
        <property type="entry name" value="GPR137"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15146">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN GPR137"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15146:SF1">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN GPR137C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0458">Lysosome</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="324"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="324"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="355" mass="40285" checksum="F8F93CFB44104CEC" modified="2018-12-05" version="1">MFSPGEEVDSDLFTSLKESSGDAISPTLELSLTTVYTVLYSFLFVFVYLQLWLILHYGHKRFSYQSVFLFLCLLWAALRTTLFSFYFKNVVQANQLQPLAYWLLYCCPVCLQFFTLCLLNLYFTQVMFKAKAKYLPELTKYKVPLRLFFLCLSIFFLVVNLTCALHAVLARVLINDSLFVLCAISLAVCIFKIAKMSSANVYLESKGTSVCQATAVGAVVILLYTSRACYNLVVVALSPQDRPSPFSYGWYSVSDQADTQVISGEGYIVFGIILFFWELLPTSLVVVFFRVQKPHQNLAPGGMINSHSFSSRAYFFDNPRRYDSDDDLSRSINSRSDRQTQSLDRESVSYAAVCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MH54</accession>
    <name>A0A3B5MH54_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MH54"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023087"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023364.1">
        <property type="protein sequence ID" value="ENSXCOP00000023087.1"/>
        <property type="gene ID" value="ENSXCOG00000017252.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156368"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004741">
        <property type="term" value="F:[pyruvate dehydrogenase (acetyl-transferring)]-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF779">
        <property type="entry name" value="SI:CH211-15P9.2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="PPM-type phosphatase" evidence="7">
        <location>
            <begin position="131"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="535" mass="59502" checksum="D2E0C1AE31AB39EE" modified="2018-12-05" version="1">MAVPSIIYNKTEEINSVSINIHLVPPKHPAVVHQLIFLLLLQVLLVLEVIMLRRSGAQTRRCWLEIQQSRYLSSAPAPPELRRLHYPASSRSRQGQAGQESGQLSPAQINRILKANEHSHTLPRGPASHGVLGFHSNTLPSNHPSEDRRSSATCLLGRGGVLFGVFDGHAGPSCAQAISQRLFYYFTVAMLPLRTLQELDRAVEEDRAVPPLLEWHKHPQDHSSLDGGAISFHSLRNYWQERLDEDDEEDNEGQYNNIAGLRVSIPGEGFSLSSPLRVALSGSTACVVHVSNGVLHVANLGDSRAVLGVQEGDGRWSALSLTNDHSAQNPEELQRILGEHPASERKTVVRHDRLLGLLLPFRAFGDVRFKWSPEMLSRLYDTRPDVLSAVSESVRTLPPHYLTPPYLSAQPEVTRHLMKPADKFLVLATDGLWELMHRQTVVQLVGDHVAGQETSGPADTTLGSLQRLLLERRDRVQSVLEDQNVATHLLRHALGDDGYGSVDQNRLARMLSLPADLARRYRDDITITVIHLNDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MH55</accession>
    <name>A0A3B5MH55_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Syntaxin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MH55"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022945"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023222.1">
        <property type="protein sequence ID" value="ENSXCOP00000022945.1"/>
        <property type="gene ID" value="ENSXCOG00000017147.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167456"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SNARE"/>
    </dbReference>
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        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14523">
        <property type="entry name" value="Syntaxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="40"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="254" mass="28306" checksum="D2E7ED78963E3545" modified="2018-12-05" version="1">MSYGSIDGGSFGSRNPFGGPTRQGYQPVTQVSPSELQDVFQEASSNIFQINVILEKSLQSLGTSRDTTELRQNHSTQQQTNKVITSTSHVIKQLSDIISGSSRQDRLRLTRLKTELSDSVQRYGDLQKKIAERSRALLPPAQKDKKKSPQSPSSDPNQEDPLFAEAAGAEEGVQAFLSEISEEDVEVLRQREEALLQIERHAGCQSNNEGPSQYGPRTRRNNKAVEEKKVLPSVGRSLDSRHPHPHYCCFSKKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MI32</accession>
    <name>A0A3B5MI32_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rhophilin, Rho GTPase binding protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MI32"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023973"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024258.1">
        <property type="protein sequence ID" value="ENSXCOP00000023973.1"/>
        <property type="gene ID" value="ENSXCOG00000017899.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153837"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051497">
        <property type="term" value="P:negative regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06712">
        <property type="entry name" value="PDZ_rhophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.160">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047138">
        <property type="entry name" value="RHPN1_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23031">
        <property type="entry name" value="RHOPHILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23031:SF5">
        <property type="entry name" value="RHOPHILIN-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017194100" description="Rhophilin, Rho GTPase binding protein 2" evidence="1">
        <location>
            <begin position="25"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="BRO1" evidence="3">
        <location>
            <begin position="109"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="2">
        <location>
            <begin position="372"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="544" mass="61282" checksum="A520EFF2BBB01271" modified="2018-12-05" version="1">YPLKQHLAELSFLFPCLLLQGCNPLAQTGRSKLQKQRASLNQQIIKQMRMRAGAENLLKYGPNSRFQVFAEVSSELFLRAETMKKNTVSFLNGFSFFCVLSDLIVKHQSSMLSHLKATFTHTPSYDMSPAMVSMLIQLMIAQAQECLFEKNALPGVLNQFTSLIKMAQEAAKVSEIYDQVHQFMVQTPIKDNVPLFWSTMSQVKTNHYRAMAHHFLASALLDHELGPNDDEDQQEKTLSQVYDQLPEGCSPLGILKNKDERERIGKAHIRCAISGHEEALRNYSLCQHLEKLEALQEILKASHRRSLDKYSDYDNEEEFVDYVEAPDIISKTEHKAEMELPAATKVKVTDFFQRLGPLAVFSAKQKWTAPRKIRVCAEDRDLGFTLKGEAPVQVVSLDPLCPAATDGLKEGDYIVAVGDKDTKWMAVSDMMRLLKDLDEEGIEIQVVSVIDNSPPMPTKSATFSGNLPKTYSMICLAHNDEDKPPKNRKVMKKSSFLSWGLKNKMKSASTLSLPTDDHSGALSWNKPIPTFPTSSTYNNNNNCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJ02</accession>
    <name>A0A3B5MJ02_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sulfurtransferase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJ02"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023732"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024016.1">
        <property type="protein sequence ID" value="ENSXCOP00000023732.1"/>
        <property type="gene ID" value="ENSXCOG00000017722.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000046773"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01448">
        <property type="entry name" value="TST_Repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01449">
        <property type="entry name" value="TST_Repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000001">
        <property type="entry name" value="Sulfurtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000008">
        <property type="entry name" value="Sulfurtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045078">
        <property type="entry name" value="TST/MPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364:SF27">
        <property type="entry name" value="SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364">
        <property type="entry name" value="THIOSULFATE SULFERTANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00380">
        <property type="entry name" value="RHODANESE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00683">
        <property type="entry name" value="RHODANESE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="6">
        <location>
            <begin position="49"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="6">
        <location>
            <begin position="198"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="321" mass="35885" checksum="ACD93B94E622C5E6" modified="2018-12-05" version="1">MLLNVCCQPPVNPRRRTHVRFDLSNMAADPTPAVVSGQWLADAIQNKLVGPKLRILDGSWYLPKTKRDTREEFNQKHIPGASFFNIDECCDKSSPYDHMLPTPSDFSRYVSDLGIGNDTHVVVYDTSDFGSYTAPRLWWTFRVFGHSSVSVLDGGFKNWVADGHPVTSDYTKPEKTDFQASLNQSWVKSYEDVLQNIKTKQVQVVDARSGGRFRGTDPEPREDILPGHFPETINIPFTSFLDSSGKHHSPENLTKLFQESGVDLEKPLWASCGSGVTACHVILAAHRLGYSGVGLYDGSWGEFFKRAPPEFIVSEGEGKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJE5</accession>
    <name>A0A3B5MJE5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJE5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023887"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024171.1">
        <property type="protein sequence ID" value="ENSXCOP00000023887.1"/>
        <property type="gene ID" value="ENSXCOG00000017842.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166815"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051000">
        <property type="entry name" value="Homeobox_DNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324:SF5">
        <property type="entry name" value="HEMATOPOIETICALLY-EXPRESSED HOMEOBOX PROTEIN HHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324">
        <property type="entry name" value="HOMEOBOX PROTEIN HHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="7">
        <location>
            <begin position="172"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="174"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="222"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="252"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="276" mass="30147" checksum="FF44898A1A51ED24" modified="2018-12-05" version="1">APRSASAAAHTMSVPLYAPTPIQPAHPTPFYIEDILGRTGSAAISSPSSSSSSCSTPVIPTPTLPSANSSFTSLISPYRTPIYEPTPIHPVLSHAALTATYGPVGAFSGSIYPFHQQQHRSMGEYAQALLRQDPLGEQRPARPFHFLHSDSCLTDSWSLSLCKEHPSVFVFQVKTWFQNRRAKWRRLKQENPQGGKREVEDDSALGRNCKSDKLTEQVGIQSLEHRQTGPTPLLTPGGHRVHSGSVQSPTELDSDVSDDSDQELDIENNSELTLNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJJ0</accession>
    <name>A0A3B5MJJ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase</fullName>
            <ecNumber evidence="19">2.7.1.60</ecNumber>
            <ecNumber evidence="20">3.2.1.183</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">UDP-GlcNAc-2-epimerase/ManAc kinase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>UDP-N-acetyl-alpha-D-glucosamine + H2O = aldehydo-N-acetyl-D-mannosamine + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30683"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17122"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="3.2.1.183"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:30684"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>an N-acyl-D-mannosamine + ATP = an N-acyl-D-mannosamine 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23832"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16062"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57666"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.60"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:23833"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="15">Amino-sugar metabolism; N-acetylneuraminate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer. Homotetramer. Homohexamer. The hexameric form exhibits both enzyme activities, whereas the dimeric form only catalyzes the phosphorylation of N-acyl-D-mannosamine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">In the C-terminal section; belongs to the ROK (NagC/XylR) family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">In the N-terminal section; belongs to the UDP-N-acetylglucosamine 2-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.60" evidence="19"/>
    <dbReference type="EC" id="3.2.1.183" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJJ0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019714"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019959.1">
        <property type="protein sequence ID" value="ENSXCOP00000019714.1"/>
        <property type="gene ID" value="ENSXCOG00000014805.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017246"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009384">
        <property type="term" value="F:N-acylmannosamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008761">
        <property type="term" value="F:UDP-N-acetylglucosamine 2-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24060">
        <property type="entry name" value="ASKHA_NBD_ROK_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000053">
        <property type="entry name" value="Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000060">
        <property type="entry name" value="Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000013">
        <property type="entry name" value="Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000600">
        <property type="entry name" value="ROK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003331">
        <property type="entry name" value="UDP_GlcNAc_Epimerase_2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964:SF149">
        <property type="entry name" value="BIFUNCTIONAL UDP-N-ACETYLGLUCOSAMINE 2-EPIMERASE_N-ACETYLMANNOSAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18964">
        <property type="entry name" value="ROK (REPRESSOR, ORF, KINASE) FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02350">
        <property type="entry name" value="Epimerase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00480">
        <property type="entry name" value="ROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="UDP-N-acetylglucosamine 2-epimerase" evidence="23">
        <location>
            <begin position="3"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00061321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00064721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00066377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00066960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00071183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00081985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="553" mass="60323" checksum="BED35CA81C0997C0" modified="2018-12-05" version="1">MCEDHSRILLAGCPSYDKLLSTYHRDDYMDIIKSWLGDKVKENDYIVALQHPVTTDIQHSIKIYGLMLDALISFNKRTLILFPNIDAGSKEMVRVMRKKGIEQHPNFQAMKHIPFEQFIQLVSHAGCMIGNSSCGVREAGAFGTPVINLGTRQTGRETGENVLHVRDADSDNKIYHALELQFGKRYPGSKIYGDGNAVPRILKFLSSIDLKEPLQKTFCFPPVKDTISQDIDHILETQSALAVDLGGTNLRVAIVCMRGNIVKKYTEPNPKTFEDRMHLILKMCKEAVQGAVRLNCRILGVGISTGGRVNPQEGIVLHSTKLIQEWSSVDLRTPISDALGLPVWVDNDGNCAALAEKKFGHGRGVDNFVTVITGTGIGGGIIHNSELIHGSTFCAAELGHIKVSLDGPECSCGSQGCIEAFASGMALQREAKRLHDEELLKMDGMDVKLAEPITAAHLISAAKNGNSKANAVLDKASLALGVGIVNILHIVNPSLVILSGVLASYYQAPVQQVMLERALFSAQSIKVVKSDLEEPALLGAASMVLDYATRRTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJR6</accession>
    <name>A0A3B5MJR6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000024027"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024312.1">
        <property type="protein sequence ID" value="ENSXCOP00000024027.1"/>
        <property type="gene ID" value="ENSXCOG00000017937.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156472"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008579">
        <property type="term" value="F:JUN kinase phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46377">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46377:SF1">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="1">
        <location>
            <begin position="7"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="2">
        <location>
            <begin position="148"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="215" mass="24105" checksum="CCA22EBF30BA1505" modified="2018-12-05" version="1">MQSLTQEIQSFSRTRLRKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSQDAAHDFGTLRKHKVSHILNVAFGVENAFPDLFIYRTVSILDHPDADLLQYMRDCCDFIQQARREKGVVLVHCNAGVSRAPAVVIGYLMSCDGQSFEDAFSLVKSARPASSPNPGFLEQLRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKE7</accession>
    <name>A0A3B5MKE7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">SHANK-associated RH domain interacting protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKE7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019994"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020241.1">
        <property type="protein sequence ID" value="ENSXCOP00000019994.1"/>
        <property type="gene ID" value="ENSXCOG00000015019.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164582"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071797">
        <property type="term" value="C:LUBAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048701">
        <property type="term" value="P:embryonic cranial skeleton morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048592">
        <property type="term" value="P:eye morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071600">
        <property type="term" value="P:otic vesicle morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097039">
        <property type="term" value="P:protein linear polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000130">
        <property type="entry name" value="SHANK-associated RH domain interactor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057468">
        <property type="entry name" value="HOIL-1/Sharpin_LTM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051628">
        <property type="entry name" value="LUBAC_E3_Ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031912">
        <property type="entry name" value="Sharpin_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770:SF45">
        <property type="entry name" value="RANBP-TYPE AND C3HC4-TYPE ZINC FINGER-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22770">
        <property type="entry name" value="UBIQUITIN CONJUGATING ENZYME 7 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25393">
        <property type="entry name" value="LTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16764">
        <property type="entry name" value="Sharpin_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="7">
        <location>
            <begin position="301"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="342" mass="36996" checksum="0D2FBB72588A3C98" modified="2018-12-05" version="1">MALSSGSWALPAPVPASSQQPACSGPSPAACCSTVLMSVRVSVSHSGIRPLCLPGAGSDSLRLQLSMDPNLAGEFRLALRDTSGNRSVVRFHPHTRLFTCRAVVKRSDVEEAPMFLHLYIPHSNAGLDGVGHLEQFRKELCSELSKVIEAGDIQAASQHASALARQKAMSELKRVGFRPSPFHLYLTYAVIHVHSSLSVVVEDASSSSSGGFYPQTSIAVLLQVFLEYGFHPRVQRWVIGQCLCSDPRSLASYGVQKDGDTAYLYLISARQARITRQLFQQDLENSLTASALPPGSSLTSQQTEWACPSCTFINKPSRPGCEICATARPDVQSCIQQVLPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLJ1</accession>
    <name>A0A3B5MLJ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitoferrin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Mitochondrial iron transporter 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Solute carrier family 25 member 37</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Mitochondrial iron transporter that specifically mediates iron uptake in developing erythroid cells, thereby playing an essential role in heme biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>Fe(2+)(in) = Fe(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28486"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027890293.1">
        <property type="nucleotide sequence ID" value="XM_028034492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLJ1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022006"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022273.1">
        <property type="protein sequence ID" value="ENSXCOP00000022006.1"/>
        <property type="gene ID" value="ENSXCOG00000016445.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114154957"/>
    <dbReference type="KEGG" id="xco:114154957"/>
    <dbReference type="CTD" id="51312"/>
    <dbReference type="GeneTree" id="ENSGT00940000158607"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035162">
        <property type="term" value="P:embryonic hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043249">
        <property type="term" value="P:erythrocyte maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048250">
        <property type="term" value="P:iron import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000027">
        <property type="entry name" value="mitoferrin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000031">
        <property type="entry name" value="mitoferrin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45758:SF4">
        <property type="entry name" value="MITOFERRIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45758">
        <property type="entry name" value="MITOFERRIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0410">Iron transport</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 18 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 19" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="18">
        <location>
            <begin position="40"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="18">
        <location>
            <begin position="138"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="18">
        <location>
            <begin position="229"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="338" mass="37065" checksum="7F3F1FF264939B76" modified="2018-12-05" version="1">MELSSGRAVARLEMSQVQSEDGEPFNESSDGDYESLPPHVSVTTHMTAGAVAGILEHTVMYPVDSVKTRMQSLQPDPNAQYKGVYEALKRIIKTEGIFRPLRGLNITMMGAGPAHALYFACYEHVKHSLSHIIQSGGNSHIANGVAGSVATVLHDAVMNPAEVVKQRMQMYNSPYRGLWDCVRTVTHTEGIGAFYRSYSTQLTMNIPFQAVHFITYELMQEQLNPHRHYNPSSHIVSGAAAGAVSAAVTTPLDVCKTLLNTQENVALSSVNISGHLSGMANAFRTVYRLGGLAAFFKGVQARVIYQMPSTAIAWSVYEFFKYFLTKQKLEQEAASGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLQ1</accession>
    <name>A0A3B5MLQ1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Immunoglobulin heavy variable 9-2</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLQ1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024496"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024790.1">
        <property type="protein sequence ID" value="ENSXCOP00000024496.1"/>
        <property type="gene ID" value="ENSXCOG00000018299.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282517"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001594">
        <property type="entry name" value="Immunoglobulin heavy variable 9-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="110" mass="12702" checksum="574AD7AEDEEB027A" modified="2018-12-05" version="1">CGTLTQSESVVKRPAESHKLTCTASEFSLTGYWMHWIRQAPGKGLEYIATDAGGNNFYSEPVRGRFTASRDYDKQQLYLQMNSLKTEDTAVYFCARKTQWRPTSPRYKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MND5</accession>
    <name>A0A3B5MND5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rab3 GTPase-activating protein catalytic subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Rab3-GAP catalytic subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MND5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022676"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022952.1">
        <property type="protein sequence ID" value="ENSXCOP00000022676.1"/>
        <property type="gene ID" value="ENSXCOG00000016938.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006705"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045700">
        <property type="entry name" value="Rab3GAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045698">
        <property type="entry name" value="Rab3GAP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026147">
        <property type="entry name" value="Rab3GAP1_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21422">
        <property type="entry name" value="RAB3 GTPASE-ACTIVATING PROTEIN CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21422:SF9">
        <property type="entry name" value="RAB3 GTPASE-ACTIVATING PROTEIN CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19533">
        <property type="entry name" value="Rab3-GAP_cat_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13890">
        <property type="entry name" value="Rab3-GTPase_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab3GAP catalytic subunit conserved" evidence="11">
        <location>
            <begin position="606"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="domain" description="Rab3GAP catalytic subunit C-terminal" evidence="12">
        <location>
            <begin position="816"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="538"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="587"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="538"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="558"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="592"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF19533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="926" mass="104703" checksum="407FE9AC2941E57F" modified="2018-12-05" version="1">MAADNDPESEVFEITDFTTASEWERFVSRVEEVLNEWKLTENYVEKTSPEKGAYINGSWGEQSLDIHFADFKFYITHHFLKEDCKTENGKSEEDTLPLAMQDLLCMHNDFPPRAHCLVRWYGLREFVVISPGANCEAIISESKCNLLLSSVSMSLSNSGCQVPMFVQIQQKWRRIYAGECQGPGVRTDFEIVHLRKVPSQYNHLSGLLDIFKSKIGCNLLPLPPVDIAIRYTYILQDWQQSSWPQQPPDFDTLLGGEVGGVEFGNLPFGACEEPISELHLATTWPHLTEGIVVDNDVYSDLDPLQAPHWSVRVRTADNLQCLLGDFITEFFKLCCRKESAEEILGRGNAEEGGQENSDISSALSKLTEPTAQVPLSKLSVSSMVHNARRRIRRHRHVDESPLHIDMLNSILLYLFPDAAVEKSSDKEKTTQANSCRTSEQEKSPDDNLFLQLKSAPCDSLTYRLALCLCMVNYSYGGLAAVAHLWQEFVLELRYRWENNYLIYGWLTGGPPDLRCCLLHQKFQMLNCCIERKRARDEARKAQEGNRERERKVSGGRASASTTRETPQGKSWDSWSDSEDEFFECLSDQGEMESSHSETEKDSSKLKADGRLHPYNNMTLLHSAELLYVPVTQEPAPMTEDQLEEQSEVLAKLGTSAEGTHLRARMQSACLLSDMESFKAANPGCVLEDFVRWYSPRDYVEEEVVDGNGSTVIRGELSARMKIPGNMWVEAWDTARVTPARRQKRLFDDTKEAEKVLHYLAMLKPGDLARHLLPCLLHAAIMKLKQEESVENIPSVEKSIQQAASQANKLLHSTNYDYKKFVISLLEEPEVVVLGAGQARIGRIIHRLFINAQRAALLAPVDDDLEPAAESRKIPDFPPPAGREILLRTCVPRPAPYSKALPQRLFCVLMKDEFRLAGAFSADTSFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNX9</accession>
    <name>A0A3B5MNX9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNX9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026433.1">
        <property type="protein sequence ID" value="ENSXCOP00000026119.1"/>
        <property type="gene ID" value="ENSXCOG00000019509.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160576"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="100" mass="11154" checksum="73C727C1331B1338" modified="2018-12-05" version="1">MFNDCFLFPGEPAQFDPTFNGPIKKRGCTDIICCILFMIVILGYIGVGMLAWLYGDPRHVLFPRNSTGWFCGNGPNNLSCFLCLLEGVAEEKWDNVVTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MR16</accession>
    <name>A0A3B5MR16_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GMP reductase</fullName>
            <shortName evidence="8">GMPR</shortName>
            <ecNumber evidence="8">1.7.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Guanosine 5'-monophosphate oxidoreductase</fullName>
            <shortName evidence="8">Guanosine monophosphate reductase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">GMPR</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides. Plays a role in modulating cellular differentiation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8 11">
            <text>IMP + NH4(+) + NADP(+) = GMP + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:17185"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.7.1.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the IMPDH/GMPR family. GuaC type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.7.1.7" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MR16"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026859"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027188.1">
        <property type="protein sequence ID" value="ENSXCOP00000026859.1"/>
        <property type="gene ID" value="ENSXCOG00000020060.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156595"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902560">
        <property type="term" value="C:GMP reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003920">
        <property type="term" value="F:GMP reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006163">
        <property type="term" value="P:purine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000012">
        <property type="entry name" value="GMP reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00596">
        <property type="entry name" value="GMP_reduct_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050139">
        <property type="entry name" value="GMP_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005993">
        <property type="entry name" value="GMPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01305">
        <property type="entry name" value="GMP_reduct_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003470">
        <property type="entry name" value="PRK05096.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43170">
        <property type="entry name" value="GMP REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43170:SF3">
        <property type="entry name" value="GMP REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000235">
        <property type="entry name" value="GMP_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8 11" id="KW-0521">NADP</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 8 10" id="KW-0630">Potassium</keyword>
    <keyword evidence="1 8" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMP dehydrogenase/GMP reductase" evidence="12">
        <location>
            <begin position="64"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="8 9">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="8">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="26"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <begin position="117"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <begin position="168"/>
            <end position="169"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="207"/>
            <end position="209"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="230"/>
            <end position="231"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="256"/>
            <end position="258"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <begin position="273"/>
            <end position="274"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="274"/>
            <end position="278"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="302"/>
            <end position="305"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000235-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000235-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="333" mass="36212" checksum="799D03C43A597E28" modified="2018-12-05" version="1">MPRVDADLKLDFKDVLFRPKRSSLKSRSEVDLRRTFTFRNSKQTYTGIPIIAANMDTTGTFEMAKVLSKVKDWKNFAADHPECLEHVAASSGSGNADLEKLCAILQAVPTLQYICLDVANGYSEYFVEFVKTVREKFPKHTIMAGNVVTGEMVEELILSGADIIKVGIGPGSVCTTRIKTGVGYPQLSAVIECADSAHGLKGHIISDGGCSCPGDVAKAFGAGADFVMMGGMLAGHDQCTGEIIEKNGKKYKLFYGMSSDTAMKKYVGGVAEYRASEGRTVEVPYRGDVENTVRDVLGGLRSTCTYVGAAKLKELSRRTTFIRVMQQSSQMFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MU81</accession>
    <name>A0A3B5MU81_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">ISL LIM homeobox 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MU81"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027056"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027389.1">
        <property type="protein sequence ID" value="ENSXCOP00000027056.1"/>
        <property type="gene ID" value="ENSXCOG00000020209.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153731"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035476">
        <property type="term" value="P:angioblast cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048675">
        <property type="term" value="P:axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031017">
        <property type="term" value="P:exocrine pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048665">
        <property type="term" value="P:neuron fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048845">
        <property type="term" value="P:venous blood vessel morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09366">
        <property type="entry name" value="LIM1_Isl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09374">
        <property type="entry name" value="LIM2_Isl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000034">
        <property type="entry name" value="Insulin gene enhancer protein ISL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000041">
        <property type="entry name" value="insulin gene enhancer protein ISL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000068">
        <property type="entry name" value="Insulin gene enhancer protein ISL-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047169">
        <property type="entry name" value="ISL1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047244">
        <property type="entry name" value="ISL1/2-like_LIM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24204">
        <property type="entry name" value="INSULIN GENE ENHANCER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24204:SF2">
        <property type="entry name" value="INSULIN GENE ENHANCER PROTEIN ISL-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7 10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 10 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 11" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="14">
        <location>
            <begin position="25"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="14">
        <location>
            <begin position="88"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="15">
        <location>
            <begin position="189"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="191"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="258"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="326"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="258"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="359" mass="40133" checksum="655FA4D52C3FB23E" modified="2018-12-05" version="1">MVDVLLNTSFLDDMGDRSKKKSGIAMCVGCGSQIHDQYILRVSPDLEWHAACLKCAECNQYLDETCTCFVRDGKTYCKRDYARLFGIKCAKCDLGFCSSDLVMRARGNVYHMECFRCSVCSRHLLPGDEFSLRDDELLCQADHGLLVDRSSAGSPLSPGNIHTRPLHIADSISVRHPPHHRNHVHKQSEKTTRVRTVLNEKQLHTLRTCYNANPRPDALMKEQLVEMTGLSPRVIRVWFQNKRCKDKKKSILMKQLQQQQQSDKTNLQGLTGTPLVAGSPIRHDNAVQGNPVEVQTYQPPWKSLSDFALQTDLDQPAFQQLVSFSESGSLGNSSGSDVTSLSSQLPDTPNSMVPSPVDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MY90</accession>
    <name>A0A3B5MY90_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Grainyhead-like transcription factor 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MY90"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027171.1">
        <property type="protein sequence ID" value="ENSXCOP00000026842.1"/>
        <property type="gene ID" value="ENSXCOG00000020043.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155788"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060561">
        <property type="term" value="P:apoptotic process involved in morphogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048839">
        <property type="term" value="P:inner ear development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021555">
        <property type="term" value="P:midbrain-hindbrain boundary morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007604">
        <property type="entry name" value="CP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057520">
        <property type="entry name" value="GRHL1/CP2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040167">
        <property type="entry name" value="TF_CP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037:SF17">
        <property type="entry name" value="GRAINYHEAD-LIKE PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11037">
        <property type="entry name" value="TRANSCRIPTION FACTOR CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04516">
        <property type="entry name" value="CP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25416">
        <property type="entry name" value="GRHL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51968">
        <property type="entry name" value="GRH_CP2_DB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Grh/CP2 DB" evidence="8">
        <location>
            <begin position="232"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="190"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="596" mass="67458" checksum="0FCA509BD1124AE8" modified="2018-12-05" version="1">MSQETDNKRLVVVVPNESSFPAVRRAYTSEDEAWRSYLENPLTAATKAMMSINGDEDSANALGLLYDYYKVPKEKRVLAIPKVAEIPEDPEKRPLLPNNPTSQNDTETLDNRVQVLKSVPVNLSLGTDHQDPKLCSSTGEAPADGDPPAVAVVKAEVYAPVFMAGPGLHYRVEGEERVVYEQSPYEVTTVNHSSYVKEDQQSPPDSPYEEETDGKYHSPSSLAADDFLFHQEGAGSFQYTLDATRSLRQKQGEGPMTYLNKGQFYAVTLSELSANKRLRHPISKVRSVIMVVFSEDKNRDEQLKYWKYWHSRQHTAKQRVLDIADYKESFNTIGNIEEIAYNAISFTWDVNEEAKIFITVNCLSTDFSSQKGVKGLPLMIQIDTYSYNNRSNKPLHRAYSQIKVFCDKGAERKIRDEERKLLRKKSKGKDGGVGVITIPKKSDVTFFKTLTDLEAQPVLFIPDVHFGNLQRAGQVDALSVLVKRMFRPSDEDLCPSPHKQSREETHKRVLLYVRKETDEVFDALMLKSPTLRGLMDAISEKYGVPTERIAKVYKKSKKGILVNMDDNIVEHYSNEDTFILSIENSADAFKVILTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MYV5</accession>
    <name>A0A3B5MYV5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transmembrane protein 145</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MYV5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025590"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025899.1">
        <property type="protein sequence ID" value="ENSXCOP00000025590.1"/>
        <property type="gene ID" value="ENSXCOG00000019117.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153981"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019236">
        <property type="term" value="P:response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053880">
        <property type="entry name" value="GPR180-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047831">
        <property type="entry name" value="GPR180/TMEM145"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019336">
        <property type="entry name" value="GPR180/TMEM145_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23252">
        <property type="entry name" value="INTIMAL THICKNESS RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23252:SF24">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10192">
        <property type="entry name" value="GPR180-TMEM145_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21892">
        <property type="entry name" value="TMEM145_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="260"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="GPR180-like N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="GPR180/TMEM145 transmembrane" evidence="8">
        <location>
            <begin position="157"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="474"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="477"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="510"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="520" mass="59032" checksum="C9DDCC78DEE8918B" modified="2018-12-05" version="1">AHTFFNNPEDWVFLTRFCFLTEFGRLDFKFRYPKSRCCQNILLYFDDSSQWPAVYKKPEKNCYEKEAVLRPENNQVINLTTRYTWSGCDDTNEEVLSCVGGRSFRSVRERWWYIALSKCGGDGLQLEYEMKLTNGQSFWTQHFSADEFGILETDITFLVIFTLVFFLSCYFAYNLKGRQLLHTTYKMFMTAAGVEVLSLMFHCVYWGLYARDGVGNGSLKILGKLLFSVSFLVFLLMLILLGKGFTVTRARISHSGSVKLSIYMTVYTITYVILFIYEAEFFDPGEVLYAYDSPAGYGLMGLQLVAYVWFCYAVLVSLKHYPEKQPFYVPFFTTYTLWFFAVPVMALIANFGIPRWAREKIVNGIQLGIHLYAHIVFLVITRPSAANKNFPYHVRTSQIGILQSSPKGGEGGESFPHHAYGNSSFLGDSQPNFTELFSIQSDPVKTVEETVARKGPDVPRNSEQKIITTAADLTMILPPPPPPIPPRPASRSPPMPPRLPSFTEYFSMQSGGGGGFGTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KK75</accession>
    <name>A0A3B5KK75_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Adenomatous polyposis coli N-terminal dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KK75"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000583"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000592.1">
        <property type="protein sequence ID" value="ENSXCOP00000000583.1"/>
        <property type="gene ID" value="ENSXCOG00000000501.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063749"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030877">
        <property type="term" value="C:beta-catenin destruction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045295">
        <property type="term" value="F:gamma-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007389">
        <property type="term" value="P:pattern specification process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.450:FF:000001">
        <property type="entry name" value="adenomatous polyposis coli protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.450">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026831">
        <property type="entry name" value="APC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026818">
        <property type="entry name" value="Apc_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12607:SF11">
        <property type="entry name" value="ADENOMATOUS POLYPOSIS COLI PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12607">
        <property type="entry name" value="ADENOMATOUS POLYPOSIS COLI PROTEIN FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11414">
        <property type="entry name" value="Suppressor_APC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82931">
        <property type="entry name" value="Tumor suppressor gene product Apc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="102"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="145"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="36827" checksum="E8A0E89047DB8A24" modified="2018-12-05" version="1">MVLSERCWLIVVSPASCLGPTSCHFLSGIPSQSRNAALCVQQEVLKQLQGTIEEESGEASGSQLELIERLKGEENWVGPLRAWFWSRQLPHRVLPSEMSLDPAGFRTRTRAPPPPSSSSSSGPNPAAFPRRGAPTAGREGHDRCLEELEKEKSLLLAELEKEEKEKDWYYAQLQNLTKRIDSLPLTENFTLQTDMSRRQLEFEARQIRSAMEEQLGSCQEMERRAQVGQVTWRSAGRQAEVCHGRHARLLLQEPVTELAERHRPGEQQQGVRPAPAARGRGGGGGTSCCWTGETGGTSWEGSAGFFLCRSRGTLSNPKNPAFCSSLCWASLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KR76</accession>
    <name>A0A3B5KR76_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Apple domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KR76"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000141"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000144.1">
        <property type="protein sequence ID" value="ENSXCOP00000000141.1"/>
        <property type="gene ID" value="ENSXCOG00000000132.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169449"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01100">
        <property type="entry name" value="APPLE_Factor_XI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.4.10">
        <property type="entry name" value="Hepatocyte Growth Factor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000177">
        <property type="entry name" value="Apple"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003609">
        <property type="entry name" value="Pan_app"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33946">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33946:SF4">
        <property type="entry name" value="COAGULATION FACTOR XI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00024">
        <property type="entry name" value="PAN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14295">
        <property type="entry name" value="PAN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00223">
        <property type="entry name" value="APPLE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57414">
        <property type="entry name" value="Hairpin loop containing domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50948">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Apple" evidence="3">
        <location>
            <begin position="19"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="189" mass="21313" checksum="FC9CFBC281C506BC" modified="2018-12-05" version="1">SISRKQAYHITAEPNCKTCQNQFFANTNIPGSDLEQEQSVSVEHCQVLCSAHPKCTYFSYSSDDFKCYLKNNLNDLAATPKDGVTSGIPAHFCQLDDSTYHESTYEGVDFRGSDIRFELMDTAELCQSKCDEDPSCQFYAYVDESFSDRRNPLMLPLCLSKLANVVSGFSLRDWKLSKLECFSLCKLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRQ5</accession>
    <name>A0A3B5KRQ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRQ5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000326"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000333.1">
        <property type="protein sequence ID" value="ENSXCOP00000000326.1"/>
        <property type="gene ID" value="ENSXCOG00000000286.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163117"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000126">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031852">
        <property type="entry name" value="Vik1/Cik1_MT-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117:SF1">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16796">
        <property type="entry name" value="Microtub_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="4">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="256" mass="27882" checksum="30159825AD4E1720" modified="2018-12-05" version="1">VSVLAGASEGYNVCLFAYGQTGSGKTYTMMGTPESIGLTPRICQFLEIYNERVRDLLRGEQKKRTSLRVREHPEKGPYVELSQHMVTDCKQAIDLLEEGIANRMTAATHNHDTSSRSHAIFTIQYTQAILENNLPSETVSKINLVDLAGERADPQYCRDRLTEGSNINKSLVTLGIVISALQNSQMSSSCQSINSVASEGDSSTLGSHSSSLSGGAGGGRRLCFIPYRDSVLTWLLKDSLGGNSKTIMIASRYCFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KT76</accession>
    <name>A0A3B5KT76_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Swi5-dependent recombination DNA repair protein 1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Meiosis protein 5 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SFR1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SFR1/MEI5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KT76"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000826.1">
        <property type="protein sequence ID" value="ENSXCOP00000000816.1"/>
        <property type="gene ID" value="ENSXCOG00000000694.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018550"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032798">
        <property type="term" value="C:Swi5-Sfr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042429">
        <property type="entry name" value="SFR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018468">
        <property type="entry name" value="SFR1/Mei5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28643">
        <property type="entry name" value="SWI5-DEPENDENT RECOMBINATION DNA REPAIR PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28643:SF1">
        <property type="entry name" value="SWI5-DEPENDENT RECOMBINATION DNA REPAIR PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10376">
        <property type="entry name" value="Mei5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="194" mass="21643" checksum="CF727ECF3D7525B0" modified="2018-12-05" version="1">MEITPKVAKLSCGSPSPCSVESSPSDASLRERLKRTRRSFISPLSVAKRLCVDSEEEEDAPPAPAGSQETITVHTMDANRTEVKLGSERLPSLAPQPSHMSSGDLALQLRKEVKEKTETLRRLKMVKMYRSKNDLTQLQTLIDKWRSCAQAALYELQSEFPIDGRKADLSELIDLFGLDDSILCFDRPEGDFTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KUE8</accession>
    <name>A0A3B5KUE8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KUE8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001259.1">
        <property type="protein sequence ID" value="ENSXCOP00000001241.1"/>
        <property type="gene ID" value="ENSXCOG00000001022.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00500000044904"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32046">
        <property type="entry name" value="G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32046:SF11">
        <property type="entry name" value="IMMUNE-ASSOCIATED NUCLEOTIDE-BINDING PROTEIN 10-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="53"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="288"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="407" mass="46276" checksum="96421624C4BB5AA8" modified="2018-12-05" version="1">EINYKPMPLEVISKSLLIQQGFPAIYQLKLKKEEIGPVTKVTLGKKNISKINKTILLVGESGAGKSTLINTMVNYTMGVKWEDKIWFQIVEEDGRNQSDSQTSDVIMYEIFGFEGKTMPFSLTIIDTPGFGDTRGIDYDTIVSHRLFDLFRSEDGVHEIHAVGLVMKATDNRVNDRLSYVLNSVMSLFGRNLENNIVALLTHSDGQRPRNVIEALEKTKIKCAKNEKNQPVYFLFDNCQNEDRTEEEDTEFLQIAEKISDKGMRALTEFLEKTAPTRVMTTAQVLNERISLMASIQNLQARIQLTEQKEMEINKIQEALKKHSEEMSRNENFTVEITEVYKAKEPTKGKKLMRLSFFEKAMCCTVCEENCHYPGCTLVPSPQHCELTSSACTICRECSFLPAIEMCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KUX8</accession>
    <name>A0A3B5KUX8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BPTI/Kunitz inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KUX8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003923"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003967.1">
        <property type="protein sequence ID" value="ENSXCOP00000003923.1"/>
        <property type="gene ID" value="ENSXCOG00000003089.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01090000260131"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000020">
        <property type="entry name" value="Collagen, type VI, alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050098">
        <property type="entry name" value="TFPI/VKTCI-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083:SF375">
        <property type="entry name" value="BPTI_KUNITZ INHIBITOR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10083">
        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="2">
        <location>
            <begin position="21"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="74" mass="8669" checksum="1E073F3E01D678E3" modified="2018-12-05" version="1">MKRHHLDKNHFFLPDFSSGGCSQPLEPGPCRQYRIRWYYDPEANACAQFWYGGCQGNTNNFENEANCRNTCVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KW70</accession>
    <name>A0A3B5KW70_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Complex I subunit B13</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Complex I-13kD-B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">NADH-ubiquinone oxidoreductase 13 kDa-B subunit</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFA5 subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KW70"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004363"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004416.1">
        <property type="protein sequence ID" value="ENSXCOP00000004363.1"/>
        <property type="gene ID" value="ENSXCOG00000003446.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008099"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006806">
        <property type="entry name" value="NDUFA5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12653:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12653">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-B SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04716">
        <property type="entry name" value="ETC_C1_NDUFA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="101" mass="11832" checksum="5521FDA19EB2F009" modified="2018-12-05" version="1">MAGLLKKRLRILYAKILASLQTMPQDAAYRKYTEQLVNERLDHVKTEPDVVKLEKKINGGQIEEVIFQAECELSLSRKMADWKPWEPLVEEAPPNQWKWPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXF2</accession>
    <name>A0A3B5KXF2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">SIM bHLH transcription factor 1b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Transcriptional factor that may have pleiotropic effects during embryogenesis and in the adult.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXF2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002306"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002340.1">
        <property type="protein sequence ID" value="ENSXCOP00000002306.1"/>
        <property type="gene ID" value="ENSXCOG00000001848.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19738">
        <property type="entry name" value="bHLH-PAS_SIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000017">
        <property type="entry name" value="SIM bHLH transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000047">
        <property type="entry name" value="SIM bHLH transcription factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000007">
        <property type="entry name" value="single-minded homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010578">
        <property type="entry name" value="SIM_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043:SF22">
        <property type="entry name" value="SINGLE-MINDED HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23171">
        <property type="entry name" value="bHLH_HIF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06621">
        <property type="entry name" value="SIM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51302">
        <property type="entry name" value="SIM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="13">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="12">
        <location>
            <begin position="77"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="12">
        <location>
            <begin position="233"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Single-minded C-terminal" evidence="14">
        <location>
            <begin position="336"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="346"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="519"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="633"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="400"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="643"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="761" mass="84829" checksum="C0F184749B333921" modified="2018-12-05" version="1">MKEKSKTAARTRREKENSEFYELAKMLPLPSAITSQLDKASIIRLTTSYLKMRIVFPQGLGEAWGHTSRTRSLDNLGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYVHPADHDEMTAVLTPHQPYHSHFVQEYEVERSFFLRMKCVLAKRNAGLTSGGYKVIHCSGYLKIRQYSLDMSPFEGCYQNVGLVAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHSCDIFHLRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGMQLSLDQMSPTKPAFPYADSHSEDRKTSKSRLTQSKAKPRVSPYPQVKASFNPERSESDQDSQWGGSPLTDSASPQLLDPSEAAEASCAYRLYPDSGSLCYGLGLSEDDLAHTQAHPHTTTCDRVRCQGGRYFLGTPQSGREMWWDTTRSVLSLPKFSVDNSEGYEITSYHGAIHGRGHWDEDSVVSSPDGGGSTSDSGERYHGDQFQSSPREPSKMETLIRATQQMIKEEENRLQQQKVLLDVSGLTKTHSPCFASSIPHHSQLTMPSVVCRGPGAPSIDLPSERLHHRYSLKALGSHDNDENTTSPVSVSRLSSPGSDGVPRPGLPLTKDYMPTDLSPHPAQAHSSPLLYPAQERQQMERQAAYALTGYSLEHLYDPENLRSYSSLACGGVQYDVASHVRMQAEQMQGHKATSVIITNGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KY06</accession>
    <name>A0A3B5KY06_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TPX2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KY06"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002516"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002550.1">
        <property type="protein sequence ID" value="ENSXCOP00000002516.1"/>
        <property type="gene ID" value="ENSXCOG00000001982.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009842"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060236">
        <property type="term" value="P:regulation of mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027329">
        <property type="entry name" value="TPX2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027330">
        <property type="entry name" value="TPX2_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009675">
        <property type="entry name" value="TPX2_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14326">
        <property type="entry name" value="TARGETING PROTEIN FOR XKLP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14326:SF44">
        <property type="entry name" value="TARGETING PROTEIN FOR XKLP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06886">
        <property type="entry name" value="TPX2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12214">
        <property type="entry name" value="TPX2_importin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPX2 central" evidence="9">
        <location>
            <begin position="254"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="TPX2 C-terminal" evidence="8">
        <location>
            <begin position="432"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="TPX2 C-terminal" evidence="8">
        <location>
            <begin position="544"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="233"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="329"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="503"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="592"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="257"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="527"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="621"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="630" mass="72358" checksum="1CCC728A0CF5AEEA" modified="2018-12-05" version="1">MEADHSGAHGDEVEKDVKSGQCFCTLVNKIRIASVGTKDLYRVEWKLMSERFVSGQSQAKCTSSNMISSEEAKKSGQAKREPAEYTTVGFCQLHSLCPRRLSSSEELELERIRSLQREVALHRRKNEASYKAALAGDPPPKKMLTSATVPKDFHFNTESRIKAPASSNASQKEVNFINQLRQPCSPAKARKGATVPKPFNLSAGHKNCKDGPGVYVSMAQLVEQFQKRTPTRYHLRSRRTQERGPSPLKGQHLHLTQPHSPQLMTRQRSRQPSVKSSAELEAEELEKLQKFKIKTVELNKKILEGPEELRKPAAKEPTVPEGFELEIEKRLQERQLNKKSQDREEPHNFKANPLPKKVLDGIVGLPEKKVTYPTVPESPAFVLKKRVRIERKVEEVKPPSPVKAISVPYFGVPFQPQLSESHQVEVCPFSFEQRDKERQALKEKKLAELQNEEVPHFKAQPLPDFNAVVLPEKKKPEPTKPEPFRLQIDERGAGRLRQWEQMVKEEQKQQEEAATFKAKPNTVTQKEPFRPKKENRTSVAVEPFQLATERRAQEWQEYERLIGEKEVLRARMEAEQRLEEEQREREEIARLRQEQVHKAQPIRHYKPVAVKKSDAPLTVPESPNFSKRFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L108</accession>
    <name>A0A3B5L108_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L108"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004010"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004054.1">
        <property type="protein sequence ID" value="ENSXCOP00000004010.1"/>
        <property type="gene ID" value="ENSXCOG00000003147.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158412"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110031">
        <property type="term" value="P:negative regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13977">
        <property type="entry name" value="STKc_PDIK1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000174">
        <property type="entry name" value="Serine/threonine-protein kinase PDIK1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000165">
        <property type="entry name" value="Serine/threonine-protein kinase PDIK1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF58">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PDIK1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="53"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="386" mass="43335" checksum="B4D0D2A1C06D5741" modified="2018-12-05" version="1">MWLCLFKLLQSLKDFSPGLMLAHGSGQFTTISDGPVLFLHSEDGRMVSSQPKYDLIQEVGRGSYGVVYEAVVKRSGARVAVKKIRCHSPENVELALREFWALSSIQSQHPNVIHLEECILQRDQLAQRMNHGSSSPLYLELVETSLKGEITFDPCCAYYLWFVMDFCDGGDMNAYLLSRKPSRKTNTSFMLQLGSALAFLHRNQIIHRDLKPDNILISQACTPTGSSEPTLKVADFGLSKVCSASGLNPEEPASVNKCFLSTACGTDFYMAPEVWEGHYTAKADIFALGVIFWAMVERITFVDVETQKELLGSYVQQGSEIVPLGEALLENPKMELLIPARKKSMNSHMKQLIREMLSANPQERPDAFELELRLVRIACRELDWDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1W3</accession>
    <name>A0A3B5L1W3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Regulator of calcineurin 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. Could play a role during central nervous system development.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RCAN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027856287.1">
        <property type="nucleotide sequence ID" value="XM_028000486.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027856288.1">
        <property type="nucleotide sequence ID" value="XM_028000487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1W3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006288"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006363.1">
        <property type="protein sequence ID" value="ENSXCOP00000006288.1"/>
        <property type="gene ID" value="ENSXCOG00000004862.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114134140"/>
    <dbReference type="KEGG" id="xco:114134140"/>
    <dbReference type="CTD" id="11123"/>
    <dbReference type="GeneTree" id="ENSGT00940000159501"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008597">
        <property type="term" value="F:calcium-dependent protein serine/threonine phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="Calcipressin-2 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006931">
        <property type="entry name" value="Calcipressin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10300">
        <property type="entry name" value="CALCIPRESSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10300:SF6">
        <property type="entry name" value="CALCIPRESSIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04847">
        <property type="entry name" value="Calcipressin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="208"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="236" mass="26699" checksum="79CFBDB49F401750" modified="2018-12-05" version="1">MLRENFKAWSNGSEAYSSDPDEDEEEEEDMVFGESDQDGVADEMMDLSDLPTALFACSVHDTVFEEDMHRERFEALFRIYDDQVTFQLFKSFRRVRINFSTPEAAARARIELHESEFNGKKLKLYFAQIQNGDEDIDKSYLAPPQPVKQFLISPPASPPVGWSQSEDATPVINYDLLCAVAKLGPGEKYELHAGTESTPSVVVHVCESETEEDEAVRPKQQIVQTRRPDGPPAVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L246</accession>
    <name>A0A3B5L246_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L246"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001869"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001897.1">
        <property type="protein sequence ID" value="ENSXCOP00000001869.1"/>
        <property type="gene ID" value="ENSXCOG00000001521.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158365"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000006">
        <property type="entry name" value="Chromobox protein homolog 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043000">
        <property type="entry name" value="CBX7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017984">
        <property type="entry name" value="Chromo_dom_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47277">
        <property type="entry name" value="CHROMOBOX PROTEIN HOMOLOG 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47277:SF1">
        <property type="entry name" value="CHROMOBOX PROTEIN HOMOLOG 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00504">
        <property type="entry name" value="CHROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="6">
        <location>
            <begin position="11"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="60" mass="7187" checksum="E4FB263B18F36437" modified="2018-12-05" version="1">MELSAIGEQVFAVESILKKRLRKGNVEYLLKWKGWPPKYSTWEPEEHILDRRLVQAFEDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2G1</accession>
    <name>A0A3B5L2G1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">G patch domain-containing protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2G1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004987"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005047.1">
        <property type="protein sequence ID" value="ENSXCOP00000004987.1"/>
        <property type="gene ID" value="ENSXCOG00000003911.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008765"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050656">
        <property type="entry name" value="PINX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149:SF9">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="3">
        <location>
            <begin position="11"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="123"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="197"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="317"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00040365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="377" mass="41623" checksum="7D5530BE9611BD0C" modified="2018-12-05" version="1">MAEAEQEKSRGLKFAEQLLLSHGWEHGKGLGRAENGISEAIKVKVKCDKGGIGHREGEQFSFHWWDHVFNKASSSLQVEAGQVGQRSHIRSQQEGAISNKKPRKFLQNKSKLYGRFVKSATLLSGQEEPEPKAPSSDSSSSDEEEEQKLDLSSTTRLSDADLMKVCGGRTAHKGARHGLTMSAKLARLEQQEAEFLTKYGKKSQTGSSPDVCDTATHPEETNRRKKKKCTESCSELSGDESPEAGFQPEKKKKKRKVKTEGGREAEEEEELIIGGNVEDQKEDGGNPSAEETEPLPSDCNRKKKKSAQHDADSAETGRSSGQSSQSDPRSQQEVEVTMATEKQRRKKSRKDKSVTEKPADDEELPPKKKKKSEGGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L3V4</accession>
    <name>A0A3B5L3V4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Lysosome-associated membrane glycoprotein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytolytic granule membrane</location>
            <topology evidence="12">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Late endosome membrane</location>
            <topology evidence="11">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Lysosome membrane</location>
            <topology evidence="14">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the LAMP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L3V4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002449"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002483.1">
        <property type="protein sequence ID" value="ENSXCOP00000002449.1"/>
        <property type="gene ID" value="ENSXCOG00000001955.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182899"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072594">
        <property type="term" value="P:establishment of protein localization to organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12087">
        <property type="entry name" value="TM_EGFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.110">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048528">
        <property type="entry name" value="Lamp2-like_luminal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048524">
        <property type="entry name" value="Lamp2-like_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018134">
        <property type="entry name" value="LAMP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002000">
        <property type="entry name" value="Lysosome-assoc_membr_glycop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11506">
        <property type="entry name" value="LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11506:SF27">
        <property type="entry name" value="LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01299">
        <property type="entry name" value="Lamp2-like_luminal"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21222">
        <property type="entry name" value="Lamp2_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00336">
        <property type="entry name" value="LYSASSOCTDMP"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00311">
        <property type="entry name" value="LAMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51407">
        <property type="entry name" value="LAMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0458">Lysosome</keyword>
    <keyword evidence="7 14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0732">Signal</keyword>
    <keyword evidence="3 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017384820" description="Lysosome-associated membrane glycoprotein 1" evidence="17">
        <location>
            <begin position="24"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Lysosome-associated membrane glycoprotein 2-like luminal" evidence="18">
        <location>
            <begin position="25"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Lysosome-associated membrane glycoprotein 2-like luminal" evidence="18">
        <location>
            <begin position="212"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Lysosome-associated membrane glycoprotein 2-like luminal" evidence="18">
        <location>
            <begin position="294"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Lysosome-associated membrane glycoprotein 2-like transmembrane" evidence="19">
        <location>
            <begin position="362"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="180"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="186"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="35"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="149"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="14">
        <location>
            <begin position="316"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF21222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="395" mass="41208" checksum="97D369FE02541CEC" modified="2018-12-05" version="1">MKVLESLAPLLIACLGILGCTQAVTLEVKEGNSTCIKAELSALLFITYTTFNSTSNVSTSLPDSTSVDAASSSCGAAGRPPWLVAVFGSGHALGLSFATNGSFYSVTNLTLQYNLSDTSLFPQSNGSGVVTVVSSSVGIWAPINTTYRCASPTTIRAGGAAVTFSAMRLEAYMPGNDLSPAESVCTADQTTTTAPPTTTTTAAPAPTPPGTPETGVFSVTNSSGTVCLLAKMGLQLNVSYVSQSQNKTVQELVNVNPNLTVTSGSCGGTTASLVLTQQTTTLNFTFSMHCFPVSPFSASNTSLDYLRSMLGRSYMCNTEQTLLVVPAFSLNTFRLQVQPFGLTASQFAAAEECQLDQDQMLIPIIVGAALAGLVLIILIAYLIGRKRSHAGYQTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4W7</accession>
    <name>A0A3B5L4W7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">receptor protein-tyrosine kinase</fullName>
            <ecNumber evidence="2">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20 31">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L4W7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002804"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002841.1">
        <property type="protein sequence ID" value="ENSXCOP00000002804.1"/>
        <property type="gene ID" value="ENSXCOG00000002226.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165103"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005005">
        <property type="term" value="F:transmembrane-ephrin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05063">
        <property type="entry name" value="PTKc_EphR_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000019">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.10:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1770:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000001">
        <property type="entry name" value="Ephrin type-A receptor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ephrin a2 ectodomain"/>
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        <property type="entry name" value="Galactose-binding domain-like"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
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    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
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    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
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    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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        <property type="entry name" value="Tumor Necrosis Factor Receptor, subunit A, domain 2"/>
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        <property type="entry name" value="Eph_TM"/>
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        <property type="entry name" value="Ephrin_rcpt_lig-bd_dom"/>
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    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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        <property type="entry name" value="SAM"/>
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        <property type="entry name" value="SAM/pointed_sf"/>
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        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
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        <property type="entry name" value="Tyr-kin_ephrin_A/B_rcpt-like"/>
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        <property type="entry name" value="Tyr_kinase_AS"/>
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    <dbReference type="InterPro" id="IPR020635">
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        <property type="entry name" value="Tyr_kinase_rcpt_V_CS"/>
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    <dbReference type="PANTHER" id="PTHR46877:SF20">
        <property type="entry name" value="RECEPTOR PROTEIN-TYROSINE KINASE"/>
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        <property type="entry name" value="EphA2_TM"/>
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    <dbReference type="Pfam" id="PF07699">
        <property type="entry name" value="Ephrin_rec_like"/>
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        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
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    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000666">
        <property type="entry name" value="TyrPK_ephrin_receptor"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00615">
        <property type="entry name" value="EPH_lbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
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    </dbReference>
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        <property type="entry name" value="RECEPTOR_TYR_KIN_V_2"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 22 24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-0675">Receptor</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="17" id="KW-0829">Tyrosine-protein kinase</keyword>
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        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
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        <location>
            <begin position="289"/>
            <end position="392"/>
        </location>
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        <location>
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            <end position="504"/>
        </location>
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        <location>
            <begin position="578"/>
            <end position="839"/>
        </location>
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        <location>
            <begin position="868"/>
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        </location>
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        <location>
            <position position="703"/>
        </location>
    </feature>
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        <location>
            <begin position="584"/>
            <end position="592"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="22 24">
        <location>
            <position position="610"/>
        </location>
        <ligand>
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        </ligand>
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        <location>
            <begin position="35"/>
            <end position="149"/>
        </location>
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        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    </evidence>
    <sequence length="906" mass="101464" checksum="D0C343B43A7DAC7B" modified="2018-12-05" version="1">MKAAGGELGWLTWSPNQGDRSGVSMVLFFYTYSICNVDEREQDNWLRTTFIQRRPSVSQVFVELQFIVRDCNSFDGASLTCKETFNLYSSDADADVGTTFRKSQFKKVATVAPDEITKKNDMRINTETKVVQSLSQKGFYLAFQDIGACIALLSVRVYYKACPATTKSLASFPETVAGGLNQQLKKVTGKCVDNAYSEDLPRIYCTADGEWVVPVGQCQCDAGFEEVEDTCQECQPGFFKAEASRDKCEPCPANTQSQNPRSLFCVCMDGFYRALTDPLTGPCSGLPSAPRGLVATPNSLTPGKLLLSWQPPEHTGGRSDITYSVECHRWEGSSHLPCGEKIRYDPANTGLLDTKVSVSELDPYLNYTFTVEVHSGVSMFPSQAMPRSFKPQSSAALNTSLHYTGEYPPKITSMRLVERDSTSLSLSWDVFPRPRVQSQPIRYELAYRKKIDVTTYVVLKLEKNSVQISDLSPGTAYLVKVQALGDDGNQGVSSLEEQFETLSEGAGRNLQRFDLKTVLLFFFLFRNRNTHTRQGPEDTYFSSPEQLKPLKTYVDPHTYEDPNVAVLKFATEIHPSHISKQKVIGAGEFGEVYRGTLKAPGKKEVAVAIKTLKPGYTEKQRQDFLSEASIMGQFSHQNIIRLEGVVTKFKHAMIVTEFMENGALDRYLRDHDGEFSSHQLVGMLRGIAAGMKYLSEMSYVHRDLAARNILVNTALECKVSDFGLSRVLEDDPEGTYTTSGGKIPIRWTAPEAIAYRKFTSASDVWSFGIVMWEVMAFGERPYWDMSNHEVMKAINEAFRLPAPMDCPSAVYQLMLQCWLQDRAKRPRFGDIVSLLDKLLRSPESLKTIADFDPRVSIRLPSTSGSDGSPFRSVSEWLESIKMSQYSENFTRAGVVTMDQVLQMKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L680</accession>
    <name>A0A3B5L680_9TELE</name>
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        <recommendedName>
            <fullName evidence="4">WNK lysine deficient protein kinase 2</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSXCOP00000006317.1"/>
        <property type="gene ID" value="ENSXCOG00000004886.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="284"/>
        </location>
    </feature>
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        <location>
            <begin position="320"/>
            <end position="343"/>
        </location>
    </feature>
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        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
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        <location>
            <begin position="165"/>
            <end position="177"/>
        </location>
    </feature>
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        <location>
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            <end position="235"/>
        </location>
    </feature>
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        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
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        <location>
            <begin position="331"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="40175" checksum="52926E5746811A14" modified="2018-12-05" version="1">MPLSLDVNPPQGRSPLAPLPLQELGATKESTSVSYAPAVRSELPQQPVVLHQPFSNVGGSKVSSLPQSPAPSQHGAGPSESDGEGRVGRGGFVDSTIKTLDEKLRNLLYQEYAPMYPSGSAAETPGSGTEYIQFPPGPDSATGGAASPAHPGGYNGDPIPPEALATSTTIGRFSVVSTEDDITQRTRCSRYSAPPDFYLDTPPPMGKSSGPPAKLAPATPSQYARSERRGSDLMKRAVAFLRRTGRSSSVQSSDSPSRHGGLPGSAYASSDNDSEMEDSDIKRELQRLREKHLKEISELQAHQRGEVELLYRRLGKVPPPGLGLLHVAPHTSRRKKSSKHRLKPGKLLSPLVQQFRNVKTKSSDSTKPGELLFMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6H6</accession>
    <name>A0A3B5L6H6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytokine-inducible SH2-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027859038.1">
        <property type="nucleotide sequence ID" value="XM_028003237.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027859039.1">
        <property type="nucleotide sequence ID" value="XM_028003238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6H6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006030"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006098.1">
        <property type="protein sequence ID" value="ENSXCOP00000006030.1"/>
        <property type="gene ID" value="ENSXCOG00000004672.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114135713"/>
    <dbReference type="KEGG" id="xco:114135713"/>
    <dbReference type="CTD" id="100136861"/>
    <dbReference type="GeneTree" id="ENSGT00940000157392"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046935">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd10718">
        <property type="entry name" value="SH2_CIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000002">
        <property type="entry name" value="Suppressor of cytokine signaling 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035887">
        <property type="entry name" value="CIS_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155:SF9">
        <property type="entry name" value="CYTOKINE-INDUCIBLE SH2-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10155">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00253">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="4" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SH2" evidence="8">
        <location>
            <begin position="48"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="9">
        <location>
            <begin position="163"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="213" mass="23995" checksum="98037258E353098A" modified="2018-12-05" version="1">MVARAATILHHEEPQSSCYSQPLPPAWHPAQDLQCITTNFQYLQTTGWYWGSISASQAREVLQSKSEGTFLVRDSSHPQYMLALSVKTRCGPTSVRIEYNRGSFWLDSTSPQLPHLQAFPDVPSLVQHYTASGQTAQDLESVRPQTKPDPVQRAAKDSGVPLKLLRPLHNVQQGFPSLQHLARLAINKHTNCTDQLPLPKPLLRFLQNYPFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L741</accession>
    <name>A0A3B5L741_9TELE</name>
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        <submittedName>
            <fullName evidence="11">Nuclear receptor subfamily 2, group F, member 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L741"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005591"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005657.1">
        <property type="protein sequence ID" value="ENSXCOP00000005591.1"/>
        <property type="gene ID" value="ENSXCOG00000004239.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165753"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904888">
        <property type="term" value="P:cranial skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032526">
        <property type="term" value="P:response to retinoic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="NR_LBD_COUP-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Coup transcription factor 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050274">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="COUPTNFACTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
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        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="10">
        <location>
            <begin position="158"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="411" mass="46250" checksum="18B42E8153480FD1" modified="2018-12-05" version="1">SPFPPALFLPSIFLFSTYHLSLSTLFHYSSLLPHFHPLTHGSSLHFHCLSPLSHLLRVTVDGVISQTVGMPEQVFSKWAFERSPHHHSSPAGGWDVCLLESLTSVCPFVSWETFVKLMQPPVFVCFWTAVQRGRTSNSQSSPGQYLTNGSDPYNSQPYLSGFISLLLRAEPYPTSRYGAQCMQGNNLMGIENICELAARLLFSAVEWAKNIPFFPDLQLMDQVALLRMSWSELFVLNAAQCSMPLHVAPLLAAAGLHASPMSAERVVAFMDHIRVFQEQVEKLKALQVDTAEYSCLKSIVLFTSDAMGLSDVAHVESIQEKSQCALEEYVRNQYPSQPNRFGRLLLRLPSLRIVSSPVIEQLFFVRLVGKTPIETLLRDMLLSVAFLLPHILTCFVLICLKYCKWRQKKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7Y3</accession>
    <name>A0A3B5L7Y3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L7Y3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006560"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006640.1">
        <property type="protein sequence ID" value="ENSXCOP00000006560.1"/>
        <property type="gene ID" value="ENSXCOG00000005081.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154824"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017071">
        <property type="term" value="C:intracellular cyclic nucleotide activated cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005222">
        <property type="term" value="F:intracellularly cAMP-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005223">
        <property type="term" value="F:intracellularly cGMP-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000072">
        <property type="entry name" value="Cyclic nucleotide gated channel beta 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000001">
        <property type="entry name" value="Cyclic nucleotide-gated channel alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000020">
        <property type="entry name" value="Cyclic nucleotide-gated channel beta 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050866">
        <property type="entry name" value="CNG_cation_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638:SF23">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL BETA-3-LIKE ISOFORM X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0140">cGMP</keyword>
    <keyword evidence="8" id="KW-0142">cGMP-binding</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="13" id="KW-0844">Vision</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="18">
        <location>
            <begin position="325"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="108"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="14"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="500" mass="56137" checksum="89A63428B6D2CC0F" modified="2018-12-05" version="1">APASAPAPAPEPTAAPASAPAPASAPAPAATPANPEQQNEAPPPPPPPIVINKYSDEQLRKVAHRMKQRLILYKEKVIDQYASSPEITPPVSEYDCIHRLSEEDRIKKEEADKKKKEEQEKKKKEAEQRKKEEEEKRKEEEKKVKLLLLFLLRVIRTIGYLLFMLHLNACFYYVASEYQGIGGTKWVYSGLGSAYLRCYYFAVRSLINIGGLNEPHTVFEITFQMTNFFVGVFVFSSLIGQMRDVIGAATAGQTYFRTSMDNTVAYMVTNNIPSMVQNRVRTWYTYTWDAQGMLDESELLDKMPLVMRTAIAVDINLATFQKIDLFKGCDQQMLVDMLLRLKSIIYLPEDFVVKKGDIGKEMYIIKSGAVQVVGGPDNSIVFVTLKAGCVFGEISLLQSSKDGGNRRTANVKAYGFANLFVLEKKDLFDILIHYPESQKVLARKGRKLMKAKGPAVATAKEEKKKGLALFGPKPPTPKLLRVFGGNFNKKMNKKNLLYKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L856</accession>
    <name>A0A3B5L856_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Single-pass membrane and coiled-coil domain-containing protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMCO4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027901222.1">
        <property type="nucleotide sequence ID" value="XM_028045421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L856"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007002"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007090.1">
        <property type="protein sequence ID" value="ENSXCOP00000007002.1"/>
        <property type="gene ID" value="ENSXCOG00000005409.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114161831"/>
    <dbReference type="GeneTree" id="ENSGT00390000015987"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027960">
        <property type="entry name" value="DUF4519"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34644">
        <property type="entry name" value="SINGLE-PASS MEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34644:SF2">
        <property type="entry name" value="SINGLE-PASS MEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15012">
        <property type="entry name" value="DUF4519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="59" mass="6640" checksum="1B64D75A881780FC" modified="2018-12-05" version="1">MRQLKGKPKKETSKDKKERKQAMQEARQQVATVVVPTVTVVVLLIVVFVYVATRPGAME</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8A9</accession>
    <name>A0A3B5L8A9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Solute carrier family 12 member 5b</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) + chloride(in) = K(+)(out) + chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:72427"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the SLC12A transporter family. K/Cl co-transporter subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8A9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007057"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007145.1">
        <property type="protein sequence ID" value="ENSXCOP00000007057.1"/>
        <property type="gene ID" value="ENSXCOG00000005448.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165387"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015379">
        <property type="term" value="F:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055064">
        <property type="term" value="P:chloride ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055075">
        <property type="term" value="P:potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000076">
        <property type="entry name" value="KCL_cotranspt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018491">
        <property type="entry name" value="SLC12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004842">
        <property type="entry name" value="SLC12A_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827:SF54">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12 MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03522">
        <property type="entry name" value="SLC12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01081">
        <property type="entry name" value="KCLTRNSPORT"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="231"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="17">
        <location>
            <begin position="44"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="18">
        <location>
            <begin position="327"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="18">
        <location>
            <begin position="459"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF03522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="680" mass="76916" checksum="8C3D1383899FB8AD" modified="2018-12-05" version="1">LYVWIQGSGAVVSSPHIHTCHGKHKWTQWNKIQNFKSESLNGFFFFLGLTGIMAGSNRSGDLRDAQKSIPIGTIAAITTTSTVYMSCVILFGACIEGVVLRDKFGEGVNGNLVIGTLAWPSPWVIVFGSFFSTCGAGLQSLTGAPRLLQAISRDGIIPFLRVFGHGKANGEPTWALLLTASICEIGIIIASLDSVAPILSMFFLMCYMFVNLACALQTLLRTPNWRPRFKFYHWALSFLGMSLCLSLMFICSWYYAIVAMGIATCIYKYIEFCGAEKEWGDGIRGISLSAARFALMRLEEGPPHTKNWRPQILVLVSMDAEQNVEQPRLLSLTNQLKAGKGLTIVGTAVQGTFLDSYSEAQRADQSLRKLMETERVKGFSQVVFSSNMRDGTSHLIQVGGLGGLKHNTVMVSWPQNWKQPECYQQFRNFIETVRETTIASLALLVPKNISSYPSNGERFTEGHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCKMRIFTVAQMDDNSIQMKKDLITFLYHLRIDAAVEVVEMLDSDISAYTYEKTLVMEQRSQILKQMHLTKNEMEREGYEYFHSHFLPIWNKLKELKGRFVFHFCRFRVLLISPVDPSTDVRRMHTAMRLNEVITKKSKEAKLVLLNMPGPPKNRVGDENYMEFLEVLTEGLNRVLLVRGGGREVITIYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8J2</accession>
    <name>A0A3B5L8J2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lebercilin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LCA5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8J2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006770"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006853.1">
        <property type="protein sequence ID" value="ENSXCOP00000006770.1"/>
        <property type="gene ID" value="ENSXCOG00000005240.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042073">
        <property type="term" value="P:intraciliary transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026188">
        <property type="entry name" value="Lebercilin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028933">
        <property type="entry name" value="Lebercilin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16650">
        <property type="entry name" value="C21ORF13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16650:SF10">
        <property type="entry name" value="LEBERCILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15619">
        <property type="entry name" value="Lebercilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lebercilin" evidence="4">
        <location>
            <begin position="167"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="372"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="439"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="600"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="687"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="66"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="450"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="483"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="509"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF15619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="755" mass="86071" checksum="0CE323BC76ADABAB" modified="2018-12-05" version="1">MEESEKVPEAIEDNRILSRQSRRSTNKESRASSGQRHRKNHQGKIHREDTMVESVGSTSKTGTCRSDPDRDRISDGEGRRSDGSLYSEDYENATQSERSLSPFSQSLTPSPVPQRGCLAKQISRSGVGRWNASRPQRPGGLPVTQHHRKGNRSQSKESTPPKDLDLVTKRMLSARLLKINELRNSLAELKQQNDELKKENRTLRQLQVRQEKALQRYDDTESEISQLLARHSNEIHVLRERLRRTQERERTAERRLKESEEQLHRSQATVTRLKKLVDQQELGTRDKLSRSFEEEKTRAQEAQRKVKDLERSIELSNNSFQRQFAAEKKKTMNSQEEVKILQEELDRLTSKLKEKERELDAKNIYSNRLVKPALRKDGESSTKQKRKSLTKAVQTEDKMSSQDFPSPPPAISDTNEYSGQSAGEYLSLKVPCSSLTFLVDKPAESNKVSQKTDEHKTRGRHEEELVREQETQQREGLNQELNVLKEKLNIKKEQEEDEDSKTSNSLFSQKEEDNYWKHGHVQEEVARWNKESTSSQQATEEARRKKEQLLAKMREIDRQNQEAQDSVFIESPLSESRKINNVASPHPPEDRNRNFSVFNHSERQSVEHGGFTAGRRALRPHTSSDDLAFGRYAPSFGHLASRVSPPPSDSKNTALEAIGLFNKDKKSSLMEQLFGAQTIPAGDSFSASNKMKVLNSPPTTNGVRSRREGLHRFSSGSSTPPPSSSIIDKALNVADSKPAIRAIPSFDDDIEELTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8Q4</accession>
    <name>A0A3B5L8Q4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Breast cancer anti-estrogen resistance protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">CRK-associated substrate</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">p130cas</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CAS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8Q4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007202"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007294.1">
        <property type="protein sequence ID" value="ENSXCOP00000007202.1"/>
        <property type="gene ID" value="ENSXCOG00000005565.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183008"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11552">
        <property type="entry name" value="Serine_rich_BCAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12001">
        <property type="entry name" value="SH3_BCAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.830:FF:000001">
        <property type="entry name" value="BCAR1 scaffold protein, Cas family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.230:FF:000001">
        <property type="entry name" value="Breast cancer anti-estrogen resistance 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000009">
        <property type="entry name" value="Breast cancer anti-estrogen resistance 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.230">
        <property type="entry name" value="Alpha-catenin/vinculin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.830">
        <property type="entry name" value="Serine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035745">
        <property type="entry name" value="BCAR1_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021901">
        <property type="entry name" value="CAS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037362">
        <property type="entry name" value="CAS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014928">
        <property type="entry name" value="Serine_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038319">
        <property type="entry name" value="Serine_rich_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10654:SF15">
        <property type="entry name" value="BREAST CANCER ANTI-ESTROGEN RESISTANCE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10654">
        <property type="entry name" value="CAS SCAFFOLDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12026">
        <property type="entry name" value="CAS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08824">
        <property type="entry name" value="Serine_rich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="11" id="KW-0729">SH3-binding</keyword>
    <feature type="domain" description="SH3" evidence="18">
        <location>
            <begin position="3"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="98"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="370"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="422"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="383"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="426"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="449"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="897" mass="99058" checksum="E7F79F6C0E49FD00" modified="2018-12-05" version="1">MNKNNILAKALYDNVAESPDELSFRKGDIMTVLERDTQGLDGWWLCSLHGRQGIVPGNRLKILVGMYDSKQQQQVTPTTPDPAVSLYANKPLPSVQYTSMHPAYSGPNPVQPSTDSVYMMPPSHGPKVSSQSLYQVPCGPNGSSPQAPPGPPKKTSAVGHRPFELPGQDIYQVPPSVGPGLSQATASAGVTGTGQDVYQVPPSWENSTKPHGKVVVPTRVGQLYVYDTLKADKDEYDVPPRHQPPAQQDIYDVPPTRQQYSTQVYDTPPMVVKGPSSGQDIYDTPAITDKNPQQTVYSFPPSVSKDVPDCQLIREETYDVPPHFAKLKHQVPAPLIPEDVYDVPPPILTEKHHRGERGAVSQEIYDIPATLRGGGLPSQDVYDFPREREDRGGERGDQYVYDVPPQVVRDAQSNAEELTKSFKRLSASSTGSTRSNHSASSLDMVPVRDTSSLPASGSIQGKPLMLDLDQAMERLSRLQQAVESSVSLMMSFITGNWRSPAHLDGNLTAIHQAADRVRVTVRDFLEFARGAVANAAQATDRLLQTKLGRQVGKMEEVFQSLIRHSQSLDSISWSPTALSTPTPGGDDLDRLIITARGIPDDAKQLASFLHGNASLLFKRTNRQQQQQLPLPPIPGEISGHMTGSGSGTYQGGEKVHIQSRPLPSPPKFTSAEEEGVDRPYEVTEEGWMEDYDYVHLQGKEEFEKNQRQLLEKGNIIRHNKTQLEQQQIKQFERLEQEVSRPINNDMTDWVPSPHHPLPASSKLCHSDRQLLLFYQEQCEQNITTVTNAIDAFFTAVNSNQPPKIFVAHSKFVILSAHKLVFIGDTLSRQAKSPEVRGRMAQSSNTLCEKLKDIVISTKTAALQYPCAGAAKEMTERVKELAGCTQQFRMGLGQLLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9M5</accession>
    <name>A0A3B5L9M5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027886374.1">
        <property type="nucleotide sequence ID" value="XM_028030573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9M5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008928"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009034.1">
        <property type="protein sequence ID" value="ENSXCOP00000008928.1"/>
        <property type="gene ID" value="ENSXCOG00000006754.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114152639"/>
    <dbReference type="GeneTree" id="ENSGT00940000165100"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000001">
        <property type="entry name" value="Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="224" mass="24333" checksum="8B366D595086A979" modified="2018-12-05" version="1">MNPIVEAVACFLGFLGWIMVGVALPNRYWRESTVDGNIITTSAVYENLWMSCSTDATGVHNCRDFPSLLELNGYIQAARALMIASIVFGTFGLVGTLVGMQCTKVCGENYVLKGRIVAIGGVFFLLQGLCTMIAVSWYAANITQQFFDQLYAGTKYELGQALYIGWSSAVLALCGGSCLLCACKLNSSSEKMSYPYQPASRRHVLSTVATSQSAQINYGRNAYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCJ7</accession>
    <name>A0A3B5LCJ7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Neurexin 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Neuronal cell surface protein that may be involved in cell recognition and cell adhesion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Presynaptic cell membrane</location>
            <topology evidence="11">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCJ7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007421"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007517.1">
        <property type="protein sequence ID" value="ENSXCOP00000007421.1"/>
        <property type="gene ID" value="ENSXCOG00000005723.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155978"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000015">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000029">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000004">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000005">
        <property type="entry name" value="neurexin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF52">
        <property type="entry name" value="NEUREXIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 13" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0357">Heparan sulfate</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0654">Proteoglycan</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Laminin G" evidence="14">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="14">
        <location>
            <begin position="78"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="274"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="14">
        <location>
            <begin position="314"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="14">
        <location>
            <begin position="493"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="15">
        <location>
            <begin position="671"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="772" mass="85827" checksum="B6021580E5619A54" modified="2018-12-05" version="1">EDYTMLGSDDFFYIGGSPNTADLPGSPVSNNFMGCLKDVVYKNNDFKLELSRLAIDRDPKITLHGDLVFRCEDVAALDPVTFEAPEAFISLPKWNTKKTGSISFDFRTTEPSGLLLFSHGRPQGPKEQKPGRELKTDYFAMELLDGYLYLLIDMGSGKTKLKASNKKVNDGEWCHVDFQREGRKGSISVNSRSMPFLSPEGSEILDLDSDMYLGGLPESKSDLILPPEVWTALLNYGYVGCVRDLFIDGKSRDVRRLSEIQSALGVSSFCTRELQKRCSSAPCGNGGLCKEGWNRYICDCTGTGYLGTNCEIEATVLSYDGSMYLKIIMPITMHTEAEDVALRFMSQRAYGLLMATTSKESADTLRLELDGGRVKLTVNLGKGPETLFAGQKLNDNEWHSVKVVRRGKSLQLSVDNVTVEGQMTGAHTRLEFHNIETGIMTERRFISVVPSNFIGHLQGLSFNSLPYPDQCKNGDISYCELNARFGMRHIIADPVTFRTKGSYLALATLQAYASMHLFFQFKTTTPDGLMLFNSGDGNDFIVVELVKGYVHYVFDLGNGPSLMKGNSDKPLNDNQWHNVVVSRDANNVHTLKIDSRTVTQHSNGARNLDLKGELYIGGVTKSMYSNLPKLIASRDGYQGCLASVDLNGRLPDLIADALHKVGQVERGCDGPSTTCTEESCYHQGVCLQQWEGFTCDCTMTSYGGSFCNDRKQTLNYLKNSVYAIESFRNSQVNWHFRIYVESLILGLARCASYLFSVYLVRSKQNISSIISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LD58</accession>
    <name>A0A3B5LD58_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enhancer of mRNA-decapping protein 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat EDC4 family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000010113"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010232.1">
        <property type="protein sequence ID" value="ENSXCOP00000010113.1"/>
        <property type="gene ID" value="ENSXCOG00000007670.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047791"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.100:FF:000001">
        <property type="entry name" value="Enhancer of mRNA-decapping protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000138">
        <property type="entry name" value="Enhancer of mRNA-decapping protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.100">
        <property type="entry name" value="conserved c-terminal region of ge- 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045152">
        <property type="entry name" value="EDC4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049404">
        <property type="entry name" value="EDC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044938">
        <property type="entry name" value="EDC4_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032401">
        <property type="entry name" value="EDC4_WD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598:SF5">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21289">
        <property type="entry name" value="EDC4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16529">
        <property type="entry name" value="Ge1_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Enhancer of mRNA-decapping protein 4 WD40 repeat region" evidence="12">
        <location>
            <begin position="109"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="287"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Enhancer of mRNA-decapping protein 4 C-terminal" evidence="13">
        <location>
            <begin position="1158"/>
            <end position="1276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="470"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="530"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="723"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="794"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF21289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1288" mass="141423" checksum="D3C8E9C57E814FA6" modified="2018-12-05" version="1">MASNSNIDIEGATQHLRDILKLDRPGSSAGNEDQRKLSFNGELNGLLGAGLLGSSLSESTRPISTDVSIQESQKICLTGDDGSTCIPIVLNNVEIEASQDSGINSKARGSNKVKIQPVAKYDWEHKYYYGRLIAVSNSFVAYAIRGANNHAMIRVLSLDFSERSLLKGFTGAVTDLAFAHLDSSLLGCVDEAGNLMVWHLTLTRSKILDEIVVHIQRPEDAPLNSHRRLIWCPYILEDSEENQDDTSQTLALLHEDGAEVWDLEVLRANNSSWPVDVSEIKDGLIIVKGHTQRVSEGALSPDGTVLATASHDGYIKFWQIYIEGGQDKPRCLHELRPHEGRPLSCLLFCDNHKRQDPEVPFWRFLITGADQNQELKLWCTVSWTCLQTIRFSPDPFNSTVLPSLKASLDLAAEYLILTDVQRKVLYVMELRQDLEKGKASFTAVSEFLLTHPVLSFGVRDVTHSRLRHTEVLPAEEESESMSTEGTQGPAESKSGIQIKLYCVHTKLFSVFNSALCSFFPAGFPDHLTDHSSDKDSGSGSQSDLRKIPSLPVPSDFLSNSGPSGGPMPKLMTPDAFMTPSTSVLIVPASPGSSASSLTIVTAISSNSDSTNRNLLFDLTDHQDKHISCVFLQTLASPSGPLGLDSPLVPDSPLLPPLASPSRARSPDVISSASTVMSQDMPEIATQTLQLQRGHGLEALQLPALQTDSMASAACALHLLTSPRAPTNNGGGSLDGSVSHTSWPAAPDITRETRNSLRDNGLAECSPYHRRSYHLPQNDSQDVGADQSDPDDEIASLASSSGNCGSRSSHRMPVKDWKSSPRASPKLKRKLKKDDRLLFGVLQFNTETQDELLMLLRSQQRELTDLCSLVETMPGTIMAQVEHVLMKHQEQEQRRLDRALAENQSRQQQLHEQLSQQLSHALSTALTNRMDKVLREELKKAVPQTISKSLEPVTGQLNNSIAAKMAAVEVTIKDIVIKVVKSKNTTDAMGRAAAEAMQGPIQAAYKDVFQSTVLPVFERGCQSMFQQINDSFKQGTQDYIQQLEAHLKSRKQREQEARDPVIGQLQQTIDGFQSCTDQLASSVSTAVRAEVQHQMQVVVGNLQESILSHLQRIVKGEVSLAMREQQAVVTSSIMQAMRSAAGTPVPTAHLDYQSQQANILQLLQQGQLNQAFQQALSATDLNLVLYVCETIDSQQVFGQTPCPLSQPVLLSLIQQLSSNLATRSQLKISYLEDAVMNLDHSEPLTRDHMSSVLAQVRSKLLAFLQQDPHSPLSKRARGLLLMLNGVVNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDZ2</accession>
    <name>A0A3B5LDZ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDZ2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007999.1">
        <property type="protein sequence ID" value="ENSXCOP00000007901.1"/>
        <property type="gene ID" value="ENSXCOG00000006065.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000171555"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF111">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="148"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="19"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="233" mass="25910" checksum="76E85509B4BD886E" modified="2018-12-05" version="1">MKTFENVIVSVICCELSWISVSVCQFHTVSVRPAEDVTLMCSNISKRVSNIFWYKMNSSPNASHISSMLSAESNVSLFDGFHFSKYNMTSNTTNLFLKINEVNFSDSGLYFCGLYAAAVPAGFTATYLEVEGKMDVIDAAFSINVSDGFLNLPCVILGCVAVFLTVVIVALVVKIRTIQTGYYETLLQNLDPHCLNYTALKSHPKAKNYARLSDEEELETDVLYAATRETHEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LE31</accession>
    <name>A0A3B5LE31_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PDZ and LIM domain protein 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May play a role in the organization of actin filament arrays within muscle cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
            <location evidence="1">Z line</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027872448.1">
        <property type="nucleotide sequence ID" value="XM_028016647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LE31"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008840"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008946.1">
        <property type="protein sequence ID" value="ENSXCOP00000008840.1"/>
        <property type="gene ID" value="ENSXCOG00000006629.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114144135"/>
    <dbReference type="KEGG" id="xco:114144135"/>
    <dbReference type="GeneTree" id="ENSGT00940000156741"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031941">
        <property type="term" value="C:filamentous actin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09450">
        <property type="entry name" value="LIM_ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06753">
        <property type="entry name" value="PDZ_PDLIM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000026">
        <property type="entry name" value="PDZ and LIM domain protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000055">
        <property type="entry name" value="PDZ and LIM domain protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031847">
        <property type="entry name" value="PDLI1-4/Zasp-like_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050604">
        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006643">
        <property type="entry name" value="Zasp-like_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF7">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15936">
        <property type="entry name" value="DUF4749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00735">
        <property type="entry name" value="ZM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="11">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="243"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="315" mass="34590" checksum="BC6533D05FF6EFAC" modified="2018-12-05" version="1">MPLNVVLDGPPPWGFRLTGGKDFNQPLTISRITPGSKASGAKLCAGDIILAIEGAPAADMLHCEAQSKIKECFNQLCLTVERNESMLWSPRVVEDGRAHPFKLNLESEQQEYKPIGTAHNRKAQPFVAAANIDDKRQVVSTSYNTPIGLYSSGNIQDAMQGQVRGLVLPKPESPRTLTNIEESDVYRMLQRDQDDPPEPRQSGSFKALQEFIDSDGTRPIVTRTVKAPTTKPAAPSGNLQKLPVCDKCGNGIVGTVVKARDKCRHPGCFVCADCDVNLKQKGYFFVESQLYCEIHARARMRPPEGHDLVTTFPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFI4</accession>
    <name>A0A3B5LFI4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the U2 small nuclear ribonucleoprotein A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027900894.1">
        <property type="nucleotide sequence ID" value="XM_028045093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFI4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006629"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006710.1">
        <property type="protein sequence ID" value="ENSXCOP00000006629.1"/>
        <property type="gene ID" value="ENSXCOG00000005136.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114161648"/>
    <dbReference type="KEGG" id="xco:114161648"/>
    <dbReference type="CTD" id="393726"/>
    <dbReference type="GeneTree" id="ENSGT00530000063405"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17045">
        <property type="entry name" value="Ubl_TBCEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000837">
        <property type="entry name" value="Tubulin folding cofactor E-like a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000115">
        <property type="entry name" value="tubulin-specific chaperone cofactor E-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000145">
        <property type="entry name" value="Tubulin-specific chaperone cofactor E-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044640">
        <property type="entry name" value="RU2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047991">
        <property type="entry name" value="TBCEL_Ubl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552:SF6">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14560">
        <property type="entry name" value="Ubiquitin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="349"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="429" mass="48651" checksum="55EB17762B14D217" modified="2018-12-05" version="1">MEPREDREGRSFVEVINEKYSPENFPYRRGPGMGVVVVPTAGLQGSPMKDRLNLPSVLVLNCCGISKAGDQAEIAAFCAHVMELDLSHNNLQDWQEISKIASSIPNLEFLNLSSNPLAGTILEPQCAEAFSRVRRLVLNNTQISWDTVMLLTQNIPKLEELFLCLNEYSSVSASSVACPTLRLLHITDNSLHNWAEVGKFGSMFPSLDTLIMANNNLASIQDNKDILARLFPNLRRINLNNSGLNRWEDIEKLNFFPKLDEVRLQGIPLLQAYTKAERRNLMIAQLPSVSNLNGSVVTDSEREDAERFFIRHYLDCPEKELPFIYHSLVTKYGKLEPLAEIDLRPRCRAQVEVHCEEKVEQLCIRLDQTVAELKKQLTTVVQLSTNSMRLYYIDKDSAFGPEELKYNNRALHSYSIQDGDEILVVPKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LI58</accession>
    <name>A0A3B5LI58_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">(Lyso)-N-acylphosphatidylethanolamine lipase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Alpha/beta hydrolase domain-containing protein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Alpha/beta-hydrolase 4</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1-O-(1Z-octadecenoyl)-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-N-hexadecanoyl-ethanolamine + H2O = 1-O-(1Z-octadecenyl)-sn-glycero-3-phospho-N-hexadecanoyl-ethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55240"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:137009"/>
            <dbReference type="ChEBI" id="CHEBI:138663"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:55241"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(N-hexadecanoyl)-serine + H2O = 1-octadecanoyl-2-hydroxy-sn-glycero-3-phospho-(N-hexadecanoyl)-serine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55236"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:138661"/>
            <dbReference type="ChEBI" id="CHEBI:138662"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:55237"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N,1-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45396"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:85222"/>
            <dbReference type="ChEBI" id="CHEBI:85229"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:45397"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N,1-diacyl-sn-glycero-3-phosphoethanolamine + H2O = N-acyl-sn-glycero-3-phosphoethanolamine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45420"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:85216"/>
            <dbReference type="ChEBI" id="CHEBI:85225"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:45421"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45400"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:85223"/>
            <dbReference type="ChEBI" id="CHEBI:85230"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:45401"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-eicosanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-eicosanoyl-sn-glycero-3-phosphoethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45392"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:85221"/>
            <dbReference type="ChEBI" id="CHEBI:85228"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:45393"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N-hexadecanoyl-1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-hexadecanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45424"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:78097"/>
            <dbReference type="ChEBI" id="CHEBI:85217"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:45425"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N-hexadecanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-hexadecanoyl-sn-glycero-3-phosphoethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:85217"/>
            <dbReference type="ChEBI" id="CHEBI:85226"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:45385"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N-octadecanoyl-1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = N-octadecanoyl-sn-glycero-3-phospho-ethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45388"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:85219"/>
            <dbReference type="ChEBI" id="CHEBI:85227"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:45389"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>an N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine + H2O = N,1-diacyl-sn-glycero-3-phosphoethanolamine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45460"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:62537"/>
            <dbReference type="ChEBI" id="CHEBI:85216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:45461"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LI58"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010300"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010423.1">
        <property type="protein sequence ID" value="ENSXCOP00000010300.1"/>
        <property type="gene ID" value="ENSXCOG00000007810.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016277"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000044">
        <property type="entry name" value="Abhydrolase domain containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF21">
        <property type="entry name" value="(LYSO)-N-ACYLPHOSPHATIDYLETHANOLAMINE LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="19">
        <location>
            <begin position="60"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00050883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00051976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00054321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00076451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00081215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="343" mass="38484" checksum="2CF8D5CCC5A3D540" modified="2018-12-05" version="1">GCTADKSSSLWSWWPSWRPTSMSLLKTTESKILACKTNKVMRFLIYNKRRLTEDASPKTPLVMVHGFGGGVGLWIRNLDALSRSRTVYAFDLLGFGRSSRPSFPSDAAKAEEQFVDSIEQWRQAVGLENMILLGHSLGGYLATSYAIQHPSRVSHLILVDPWGFPERTKPETQQSEDQGAEAVKRPTIPRWARAIVSILSLFNPLAVIRAAGPWGPGLVNRFRPDFKRKFEDLFDDDTMTQYIYHCNAQTPSGEVGFRAMSETLGWAKRPMLQRVHLLPTSMPVTMLYGARSWVDSSSGDRAAEIRGKAHTKVLLVDDASHHVYADQPDDFNKVVENICKSVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIY0</accession>
    <name>A0A3B5LIY0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein TMEM103</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ELP6</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP6 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIY0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007829"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007927.1">
        <property type="protein sequence ID" value="ENSXCOP00000007829.1"/>
        <property type="gene ID" value="ENSXCOG00000006012.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011734"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
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        <property type="entry name" value="Elongator acetyltransferase complex subunit 6"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 6"/>
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        <source>
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        <source>
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        <source>
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        <source>
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    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="278" mass="30960" checksum="4BF7BC3F351138C7" modified="2018-12-05" version="1">MFTELNSILNTSPDSYTRGEFILVSDRQSDASFLIHHFLSFYLRARCKVCFLGLAQSFCHYSAVSQRLGVSLTQAKEKGQLVFFEGLKECLSVLTPKENNVESEAMNFLRDPPVGLRSLYEFVRSSVIGSDNMEEGAEQWGPSVLLVDDLSVLLSLGVDVGAVLDFSHYCRALVCSQVQGNMVMLVRCNGEEDDDDDDDDDGDIGSDVLLKGLTHQCNLNLHVQGLPTGYCKDIHGQMEVCWKRRQGDAPHMQKNLFQYKVYDKGASFFARGTSSAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJ61</accession>
    <name>A0A3B5LJ61_9TELE</name>
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        <recommendedName>
            <fullName evidence="10">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
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    <dbReference type="AlphaFoldDB" id="A0A3B5LJ61"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
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    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
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        <location>
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            <end position="21"/>
        </location>
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            <begin position="7"/>
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        </location>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="160" mass="17801" checksum="B51FDDD1406E05FC" modified="2018-12-05" version="1">TTTLISPLFFVLTLRTNHVELVQEPNSETLQPGSSVTFNCTVDTEACEGGHTVLWYRHGAYQAVLRTHTSQCEITSAQQKSSQACVYHLQKNNLSSSDAGTYYCVVASCGKMLFGSGSELLIRRHAEERLSQTGTFFWLSVFRCGILLLFVIICVFSNHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJD8</accession>
    <name>A0A3B5LJD8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Endothelin receptor type B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Endothelin receptor non-selective type</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
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            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJD8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007994"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008092.1">
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        <property type="gene ID" value="ENSXCOG00000006140.1"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048066">
        <property type="term" value="P:developmental pigmentation"/>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="P:regulation of blood pressure"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:vasoconstriction"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7TM_GPCR_Srsx"/>
    </dbReference>
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        <property type="entry name" value="Endthln_rcpt"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46099:SF3">
        <property type="entry name" value="ENDOTHELIN RECEPTOR TYPE B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00366">
        <property type="entry name" value="ENDOTHELINR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="9" id="KW-0675">Receptor</keyword>
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    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017446167" description="Endothelin receptor type B" evidence="14">
        <location>
            <begin position="20"/>
            <end position="152"/>
        </location>
    </feature>
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        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="92"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00032475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="152" mass="16790" checksum="57802E27145E29A7" modified="2018-12-05" version="1">MTTRLCFLLLVAHMTVASAQPEPKNQIPQAPLSVTQRLGLVQRRAQDPARPNITMPRSRQPPMCTGPTEIRDTFKYINTVVSCLVFVVGIIGNSTLLRIIYKNKCMRNGPNILIGSLALGDLLHLIVGIPINVYKVRDAPPGALRNSQPLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJM0</accession>
    <name>A0A3B5LJM0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJM0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008074"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008172.1">
        <property type="protein sequence ID" value="ENSXCOP00000008074.1"/>
        <property type="gene ID" value="ENSXCOG00000006203.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000235424"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050488">
        <property type="entry name" value="Ig_Fc_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481:SF64">
        <property type="entry name" value="FC RECEPTOR-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481">
        <property type="entry name" value="IMMUNOGLOBULIN FC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="17"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="144"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="310" mass="34542" checksum="B172054C65A4ED3F" modified="2018-12-05" version="1">MLCYLKNMQIIGHAKEPTATLTAGSTTIPVGGSVTLSCSVEPSAGWKYRWSRRTSDTSEFLLEKCPKPNPFFSLCLILCFCSHLINSYSDWEYKLNRNNKSYLSYIPHKSNTLLLLSGEYQCCGCRRSSDQTKCSNIVTVSGKPTATLTAGSTTIPVGGSVTLSCSVEPSAGWKYRWFRRTLDTPEAEITTNNEDNREITVTQGGIYKCAGERENPVFNSLLSHDVTIKTTCEFLLENFLWSVLETEIKCAISTKGFECSSEPDAPKRPVHNSSTCCQLPVSKTHFVQCPYSERIFLLIDFSCLCDELFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LK32</accession>
    <name>A0A3B5LK32_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LK32"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012438"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012589.1">
        <property type="protein sequence ID" value="ENSXCOP00000012438.1"/>
        <property type="gene ID" value="ENSXCOG00000009399.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007686"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051362">
        <property type="entry name" value="WD_repeat_creC_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14107:SF15">
        <property type="entry name" value="DYSTROPHIA MYOTONICA WD REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14107">
        <property type="entry name" value="WD REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="268"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="331"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="420"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="443"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="574" mass="63008" checksum="D1FA5F48130AEE0C" modified="2018-12-05" version="1">YVSPNKLLSSSSSNAAANSSPAGFLPPVRVSMVKLQPEDPSEESERVCFNIGRELYFYTYTNIKKAVDLSKPIDKRIYKGTQPTCHDFNQYSATAESVALIVGFSAGQVQYLDPIKKETSKLFNEELPTFHSLFCVYFNCHNIQTLWYFLPEFQRLIDKSKVTCLKWLPKSENLFLASHASGHLYLYNVDHPCGTTAPQYSLLRQGEGFAVYACKSKTPRNPLLRWAVGEGGLNEFAFSPDGVHVACVGQDGCLRVFHFDSMELQGVMKSYFGGLLCVSWSPDGKYLATGGEDDLVTVWSFAESRVVARGHGHKSWVNVVAFDPFTTSLEDDEPMELSGSEEDLHQGAQNNSTHFGRVRTSSTLSRLSRHSSKGGGSPSVTYRFGSVGQDTQFCLWDLTDDVLYPRLPLSRAFTNTFGVEGHHHPPGANTTSANPPTLPLPLPRSLSRSNSLPHPAVANASKGPGSGSGSVEKEHKRYHSLGNISKSNDKINVAPRSNRLDGAKVLGTTLCPRMHEVPLLEPLVCKKIAHERLTVLVFMDDCIITACQEGLICTWARPGKAVPQNGNSPSGTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKD3</accession>
    <name>A0A3B5LKD3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Dispatched homolog 1 (Drosophila)</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the dispatched family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKD3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012533"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012684.1">
        <property type="protein sequence ID" value="ENSXCOP00000012533.1"/>
        <property type="gene ID" value="ENSXCOG00000009477.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157407"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021984">
        <property type="term" value="P:adenohypophysis development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008015">
        <property type="term" value="P:blood circulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048701">
        <property type="term" value="P:embryonic cranial skeleton morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048839">
        <property type="term" value="P:inner ear development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042694">
        <property type="term" value="P:muscle cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031290">
        <property type="term" value="P:retinal ganglion cell axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007519">
        <property type="term" value="P:skeletal muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1640.10:FF:000011">
        <property type="entry name" value="Dispatched RND transporter family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052081">
        <property type="entry name" value="Dispatched_Hh_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45951:SF4">
        <property type="entry name" value="PROTEIN DISPATCHED HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45951">
        <property type="entry name" value="PROTEIN DISPATCHED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="462"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="502"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="531"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="9">
        <location>
            <begin position="18"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="578"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="641"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="739"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="882"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="650"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="739"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="764"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="790"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="895"/>
            <end position="905"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="905" mass="100790" checksum="ADAFB0FF8B182713" modified="2018-12-05" version="1">MRVGGGSCISPCTWPPTVSVTLHHAALSMFVTSFTTAAAFYANYVSNITAIRCFGVYAGTAILVNYILMVTWLPAVVVLHERYLPNLFICSKPPQQQQGNCFTQVFWASVCHKIHKCLFTLSEGSRIFFEKVLPCVVIKLRYLWLFWFLAITVGGAYVVCVNPKMKLPSLELAEFQVFRSSHPFERYDAEYKKLFMFERVNHGEDLHMPITIIWGVTPEDNGDPLNPKNKGKLMLDSSFNIASPASQLWILNFCQKLRNQSFVFQSEDQDFTSCFIETFKQWMENQDCEETSVYPCCSQSTFPYKKDVFQLCIKRAIMELDRSTSYHLDSKTPGPRFDINDTIRAIVLEFQSTYLFTLAYEKMYHFYREVDAWITEELRFAPEGLSHGWFISNLEFYDLQDSLSGGTLVAMALSVAVAFSVMLLTTWNVIISLYAILSIAGTIFVTVGSLVLLGWELNVLESVTISVAVGLSVDFAVHYGVAYRLAPEPGREGKVIFSLSRMGSAIAMAAVTTFVAGAMMMPSTVLAYTQLGTFMMLIMCISWAFATFFFQCMCCCLGPQGTCGQIPLPKKLQCQAFSEDAPSSPSPEGKYQLDSRGGEAEHYELEPLASYQKTGVKPEEKEEAEEELGTRLCNGAPSHANHTMSIPHIHSNDTDRKTRPENGLVTSLSTPSRCHYTPNNSLLPPNVDAVYSHMDCCVHYVHCPLTHFHHCSQGRAVGHRQGPAHTCHLRKYCVQPASNQDIGQASGSPSAPQTPDKETKHLSPGSTQNGNSQVPTRPNGQCPSPTALTAHRENQRTKEREHCCDKNPASAINTDSITDIDDCRKIPGNQERPESYPVTREQSVKKCKRNSKRAQASSASPKRLYSFNGTLKVKCNSASEFSLPKSEASVPPVAVNTQPSSESLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKR4</accession>
    <name>A0A3B5LKR4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">PurE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the AIR carboxylase family. Class II subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the SAICAR synthetase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKR4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011512"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011644.1">
        <property type="protein sequence ID" value="ENSXCOP00000011512.1"/>
        <property type="gene ID" value="ENSXCOG00000008689.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010172"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01416">
        <property type="entry name" value="SAICAR_synt_Ade5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000183">
        <property type="entry name" value="Probable multifunctional protein ADE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000020">
        <property type="entry name" value="Probable multifunctional protein ADE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1970:FF:000006">
        <property type="entry name" value="Probable multifunctional protein ADE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02045">
        <property type="entry name" value="PurE_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033626">
        <property type="entry name" value="PurE_classII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050089">
        <property type="entry name" value="SAICAR_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43599:SF11">
        <property type="entry name" value="BIFUNCTIONAL PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE_PHOSPHORIBOSYLAMINOIMIDAZOLE SUCCINOCARBOXAMIDE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43599">
        <property type="entry name" value="MULTIFUNCTIONAL PROTEIN ADE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52255">
        <property type="entry name" value="N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01058">
        <property type="entry name" value="SAICAR_SYNTHETASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurE" evidence="13">
        <location>
            <begin position="276"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="435" mass="48121" checksum="B0B392DF5F4A5293" modified="2018-12-05" version="1">MAFIERSPSSNFKCFTLNIGQKLSEGKTKQIFELVDQPGLVLVQSKDQITAGNAARKDQMEGKAAIANKTTCCVFKLLQESGIKTAFVQQHSETAFTAAHCEMIPIEWVCRRVATGSFLKRNPGVKEGYRFTPLKMEMFFKDDANNDPQWSEEQVLAAKFSLAGLTIGQCEVDIMNRSTVAIFEILEKAWTTQNCTLVDMKIEFGVNVKTREIVLADVIDNDSWRLWPAGDRSQQKDKQVYRDLKEVTPEAMQMVKRNFEWVSERVQLLLEPQASGRVVVLMGSTSDMAHCEKIKKACTSYGLPCILRVTSAHKGPDETLRIKAEYEGDGVPTIFVAVAGRSNGLGPVMSGNTAYPVINCPPLTPDWGAQDVWSSLRLPSGLGCSTILAPDAAAQFAAQIIGLSNHLVWCKIRASMLNTWVSLKLADQKLQACSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLR2</accession>
    <name>A0A3B5LLR2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Leucine-rich repeat-containing protein 51</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027885591.1">
        <property type="nucleotide sequence ID" value="XM_028029790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLR2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008809"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008915.1">
        <property type="protein sequence ID" value="ENSXCOP00000008809.1"/>
        <property type="gene ID" value="ENSXCOG00000006715.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114152079"/>
    <dbReference type="KEGG" id="xco:114152079"/>
    <dbReference type="GeneTree" id="ENSGT00510000047925"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46545">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46545:SF1">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="183" mass="21052" checksum="3903AE20B01FE3E9" modified="2018-12-05" version="1">MYGPPIDLSFKDLNNVTDALAEIPRRPLRPLKIDMNNKYLSQSMRLNNNNITNLVGLEFILNHFLAQPSSLGWLDLSCNKITSIEHVLCELKELRVLYLHGNDIWSLTEISKLGELNYLHTITLHGNGIENVKGYRNYVISMLPHLKRMDFSAVTRQEKVLASIWPTNIKRPRTRPQDASEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LM08</accession>
    <name>A0A3B5LM08_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shroom family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LM08"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008894"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009000.1">
        <property type="protein sequence ID" value="ENSXCOP00000008894.1"/>
        <property type="gene ID" value="ENSXCOG00000006784.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155212"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06750">
        <property type="entry name" value="PDZ_shroom2_3_4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000100">
        <property type="entry name" value="Shroom family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027685">
        <property type="entry name" value="Shroom_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15012">
        <property type="entry name" value="APICAL PROTEIN/SHROOM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15012:SF32">
        <property type="entry name" value="PROTEIN SHROOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="8">
        <location>
            <begin position="50"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="147" mass="16453" checksum="C18749E6EF4A992B" modified="2018-12-05" version="1">MNTPHFSACVCVSEHSCLWNHRVDSFRNRLLRQSLLETLSGLLSARLQGGAPWGFTVQGGLEHGQPLIISKVEDGGKADSVERPLLVGDEIIIINDVELSGYRQEAIALIKGSFKTLKLTVRRFLFIYCHNYFKMKINKSGTNSSFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LM49</accession>
    <name>A0A3B5LM49_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">FAM3 metabolism regulating signaling molecule A</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LM49"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009040.1">
        <property type="protein sequence ID" value="ENSXCOP00000008934.1"/>
        <property type="gene ID" value="ENSXCOG00000006670.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183004"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039220">
        <property type="entry name" value="FAM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039477">
        <property type="entry name" value="ILEI/PANDER_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14592">
        <property type="entry name" value="UNCHARACTERIZED FAM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15711">
        <property type="entry name" value="ILEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52031">
        <property type="entry name" value="GG_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0430">Lectin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="ILEI/PANDER" evidence="8">
        <location>
            <begin position="38"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF15711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="161" mass="18219" checksum="A260630BA205F842" modified="2018-12-05" version="1">MCLGFTLYCDTMCKSSRKRNTSAMHCKLMSSVKNNVGRGLNIALVNGVTGELLETRAFDMWAGGNEIHLHVIQFSNNCFVFFVYFGRLNDESRRLFEELGSTAVKELAFRDSWVFVGAKGIENKSPFEQRMKNSKSSNKYEGWPESLEMDGCIPLRPPLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNM1</accession>
    <name>A0A3B5LNM1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WD repeat-containing protein 26</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNM1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009444"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009555.1">
        <property type="protein sequence ID" value="ENSXCOP00000009444.1"/>
        <property type="gene ID" value="ENSXCOG00000007174.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153634"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000087">
        <property type="entry name" value="WD repeat-containing protein 26 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054080">
        <property type="entry name" value="TPR1-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051350">
        <property type="entry name" value="WD_repeat-ST_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22838">
        <property type="entry name" value="WD REPEAT PROTEIN 26-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22838:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21889">
        <property type="entry name" value="TPR1-like_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CTLH" evidence="8">
        <location>
            <begin position="117"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="292"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="503"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="557" mass="61746" checksum="37E3DD7395F8EDB1" modified="2018-12-05" version="1">MQSNGAGQGQESSELPCLNSAQNGESSSSAGGTHSNGLLSNADNGNGVGTSNGSSVGPCSGTSATSTASGSEVGSLKKKKRLSQTEEDVIRLIGQHLHGLGLQTVDLLMQESGCRLEHPSASKFRNHVMEGEWDKAENDLNELRALMHSPNAIRMKFLLLQQKYLEYLEDGKVLEALQVLRGELTPLKYNTDRIHVLSYLMCSHADDLRAKAEWEGKGTASRSRLLDKLQYLPPSVMLPPRRLHTLLRQAVELQRDRCLYHNTKLDNNLDSVSLLLDHVCSESHQLKLLRTLEGHAYGVSYLAWSPDDTYLIACGPDDCSELWLWNVQTGELRTKMSQSHEDSLTSVAWNPDGKRFVTGGQRGQFYQCQDLDGNLLDSWEGVRVQCLWCLSDGKTVLASDTHQRIRGYNFEDLTDRNVQEDHPIMSFTVSKNGRLALLNVATQGVHLWDLQDRVLVRKYQGVTQGFYTIHSCFGGHNEDFIASGSEHKVYIWHRRGELPIAELTGHTRTVNCVSWNPAIPGLMASASDDGTVRIWGPAPFLDTQEADGLNNSSNMDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LP79</accession>
    <name>A0A3B5LP79_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Calcitonin peptide-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LP79"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013893"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014063.1">
        <property type="protein sequence ID" value="ENSXCOP00000013893.1"/>
        <property type="gene ID" value="ENSXCOG00000010520.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177875"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021116">
        <property type="entry name" value="Calcitonin/adrenomedullin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00214">
        <property type="entry name" value="Calc_CGRP_IAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017208467" description="Calcitonin peptide-like domain-containing protein" evidence="3">
        <location>
            <begin position="23"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="17"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="97"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="72"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR621116-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="12904" checksum="BF403B6C14129F20" modified="2018-12-05" version="1">GAKMRIPMLLTIPLYFSSGSSSSDDKQENALPDKESRSLPELIADPFSGLMGARPERGLTATNSHHVQKRKCDLGTCHRERLGHDLMVLAGRKEKKTDVGENSFGKREIHLPPAQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPC7</accession>
    <name>A0A3B5LPC7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ataxin 7</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPC7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013948"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014120.1">
        <property type="protein sequence ID" value="ENSXCOP00000013948.1"/>
        <property type="gene ID" value="ENSXCOG00000010558.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157279"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021707">
        <property type="term" value="P:cerebellar granule cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021702">
        <property type="term" value="P:cerebellar Purkinje cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043009">
        <property type="term" value="P:chordate embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046530">
        <property type="term" value="P:photoreceptor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060042">
        <property type="term" value="P:retina morphogenesis in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052237">
        <property type="entry name" value="Ataxin-7-like_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013243">
        <property type="entry name" value="SCA7_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15117">
        <property type="entry name" value="ATAXIN 7 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15117:SF2">
        <property type="entry name" value="ATAXIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08313">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51505">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCA7" evidence="2">
        <location>
            <begin position="277"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="518"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="635"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="477"/>
            <end position="490"/>
        </location>
    </feature>
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        <location>
            <begin position="529"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="574"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="645"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="675"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="697"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="769" mass="81326" checksum="7C05CC15D9DCB170" modified="2018-12-05" version="1">MSERAEDDVRGEQRRAARQQLKQQQIQRGEGSTAMATVAERRSLPSPEIMLGQPWSSWVDAAKLHGHDGAESEESFKDLGKNREAMRLCREDMPIFGQCPAQDDFYLVMCSHCGHVVKPQAFQAHYGKCRSKADCHDIVSLGLQNLSQGLGNALSRFSHFISVTFSSSPAVKVEKMHLKVESSAKPLQASSAPATSSSSSSTPSSNTTVSTAAITAPTSLSSSSLPSSSSSALKPGLNCPLIQKPPLLVSGPIPNGKGLPADKKQDSSTSSKRHVNKKVSEREFNPDIHCGVMDATGRKPCTRSLTCKTHSLGHRRAVPGRRKRFDILLAEHKNRTRERDRERELHQNHSQQTAPLRDPHPSPQLAPSHDTHLVSHAPRSVCSNALVFCSSGDPSVVHESLHHPQPTPDVLSRPSSDEGENEEREDGTDKLDFLYSGYHPRPAAVSPNSSLASLHKTSTNSHGSTPSSGFLSPPATLPQSPYSQSVESRSSLSYGTTLNAHSSLQGGTEHPAYGTTQARQVSSLPQMPSAHLSSSSSSSAASLASSRALKSRSSSSTNNSSSTSFSSGKKRKNSSLLSSSHCSSESSSSYAGAPSSSSSSSSFKKNCANVGSSGSTYHHGSLGASSSVGLNCGPSMRTNSLSLKAEPSAGGSSGPPARGPPSGSPAESIKRMSVVMNSSDSTLSLGPFVHHQPASSSDHHANFSHHSADGRLEAKKRKSSSASGGTGPGRPKMTKSPALNNIHGKHSRSIPGTPGLPNNSHLHQPKARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPR3</accession>
    <name>A0A3B5LPR3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPR3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012894.1">
        <property type="protein sequence ID" value="ENSXCOP00000012740.1"/>
        <property type="gene ID" value="ENSXCOG00000009619.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155796"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051493">
        <property type="term" value="P:regulation of cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000030">
        <property type="entry name" value="Serine/threonine-protein kinase TAO2, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000029">
        <property type="entry name" value="Serine/threonine-protein kinase TAO2, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051234">
        <property type="entry name" value="TAO_STE20_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47167">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TAO1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47167:SF8">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE TAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="28"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="316"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="557"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="891"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="332"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="393"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="409"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="567"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="902"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="948"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="988"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1006" mass="116000" checksum="A2EAAE9E616B64A3" modified="2018-12-05" version="1">MPNNSRAGNLKDPDIAELFFREDPEKLFTDLHEIGHGSFGAVYFARDVRSNDVVAIKKMSYIGKQSNEKWQDIIKEVKFLQRIQHPNSIEYKGCYLREHTAWFVYFFSPYFHKKPLQEVEIAAITHGALHGLAYLHSHNMIHRDIKAGNILLTEPGQVKLADFGSASIACPANSFVGTPYWMAPEVILAMDEGQYDGKVDIWSLGITCIELAERKPPLFNMNAMSALYHIAQNDSPVLQSTEWTDYFRKFVDSCLQKLPQERPNSEELLKHAFIQRERPESVLIDLINRTKDAVRELDNLQYRKVKKILFQEAHNGSMNEAQDDDEEADPSVSGTGTVNSVGSNQSIPSMSISASSQSSSVNSIPDAGEDKSEPDMDGDGTVMSNSSVINLKPEQEARKEESEPHGRGTTEPQAAPTQTQRTRRNREDFATIRTASLTRQMKEHEQESELREQILGYKRMRRQHQKQLLALENKLKTEKDEHRLRLDKELETQRNNFAQELEKVVKKHQASMEKDAKIFSNDEKKFQHHIQGQQKKELNSFLESQKREYKLRKEQLKEELNENQSTPKKEKQEWLSKQKENFQNLQAAEEANLQRRQRQYLDLECRRFKRRILMARHKIEQQHLREELNKRQTQKDLEHAMLLRHHESMQELQFRQLNNIQKTRVELIRLQHQTELTNQQEYNKRRERELRRKHGMEVRQQPKSLKNKELQIKRQFQDTCKIQTRQYKALRNHLLETTPKSEHKAVLKRLKLEQHRKLAILAEQYDHSISEMFSTQALRLDVTQESQCQALQIQLQQELELLNAYQSKIKMQTDAQHAREKNDLEQRVSLRRALLEQKIEEEMQSLQNERTERIQNLLERQAREVEAFDSESMRLGFNNMMLADLSPEALSNSFPGAPGGFSQHQQLRPSSSSQGPRWGSRGSSLSSNHRGSHHHYQSSLGGSPMQQGWGGHSSAGGGGGGGAGQMQWGHPQGGALQAMGGRVEPGMSRSSSVTSQISNGSQFSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQ88</accession>
    <name>A0A3B5LQ88_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQ88"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012940"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013100.1">
        <property type="protein sequence ID" value="ENSXCOP00000012940.1"/>
        <property type="gene ID" value="ENSXCOG00000009799.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00800000124176"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032300">
        <property type="term" value="C:mismatch repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF47">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="123" mass="13434" checksum="DB19FF862D42CD12" modified="2018-12-05" version="1">MIRCLPEEIQGKIRSGVAIPSLQQCVEELILNSIDAGATCVGVRIDMEAFKVQVIDNGSGMTVEDMERVGTRYHTSKCSCIEDLENLKCYGFRGEALASIVSLATCCYTEVQLHTDGVCLLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LR02</accession>
    <name>A0A3B5LR02_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LR02"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011972.1">
        <property type="protein sequence ID" value="ENSXCOP00000011836.1"/>
        <property type="gene ID" value="ENSXCOG00000008954.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000263473"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784:SF3">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 4 MEMBER K-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="24"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="124"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="240" mass="28656" checksum="67F10D38E6954DE1" modified="2018-12-05" version="1">EKELCYFPTLPVSVSLLSLFLYMKTWLEAQSYCREKHTDLISGTKQLQDEEVKKLMNSSSTYPHIGLFRDTWRWSDGNSFSFRHWNNDFNYPQSNSGQCAMTVFNDGGRWRNVSCAERKPFICYDDKVILITENKTWEDALYYCRDHHHDLVTITNMDDQRWIQEKVKNASTDYVWTGLRYTCTLDLWFWVSDEVVIYKNWTEGEPMDDCDMSGAMETKRNHQWFKRNDDELLNFICSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRU7</accession>
    <name>A0A3B5LRU7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="31">Carboxylic ester hydrolase</fullName>
            <ecNumber evidence="31">3.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="34 35">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = di-(9Z)-octadecenoylglycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38575"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:75945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:38576"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1,2,3-trioctanoylglycerol + H2O = dioctanoylglycerol + octanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47864"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25646"/>
            <dbReference type="ChEBI" id="CHEBI:76978"/>
            <dbReference type="ChEBI" id="CHEBI:88066"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:47865"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>12-(9Z-hexadecenoyloxy)-octadecanoate + H2O = 12-hydroxyoctadecanoate + (9Z)-hexadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52072"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:84201"/>
            <dbReference type="ChEBI" id="CHEBI:136312"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:52073"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>12-(9Z-octadecenoyloxy)-octadecanoate + H2O = 12-hydroxyoctadecanoate + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52060"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:84201"/>
            <dbReference type="ChEBI" id="CHEBI:136302"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:52061"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>12-hexadecanoyloxy-octadecanoate + H2O = 12-hydroxyoctadecanoate + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52056"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83677"/>
            <dbReference type="ChEBI" id="CHEBI:84201"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:52057"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>12-octadecanoyloxy-octadecanoate + H2O = 12-hydroxyoctadecanoate + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:84201"/>
            <dbReference type="ChEBI" id="CHEBI:136330"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:52081"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>13-(9Z-hexadecenoyloxy)-octadecanoate + H2O = 13-hydroxy-octadecanoate + (9Z)-hexadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52076"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:136304"/>
            <dbReference type="ChEBI" id="CHEBI:136315"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:52077"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>13-(9Z-octadecenoyloxy)-octadecanoate + H2O = 13-hydroxy-octadecanoate + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:136303"/>
            <dbReference type="ChEBI" id="CHEBI:136304"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:52065"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>13-octadecanoyloxy-octadecanoate + H2O = 13-hydroxy-octadecanoate + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52084"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:136304"/>
            <dbReference type="ChEBI" id="CHEBI:136335"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:52085"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>5-(9Z-hexadecenoyloxy)-octadecanoate + H2O = 5-hydroxy-octadecanoate + (9Z)-hexadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52092"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:136369"/>
            <dbReference type="ChEBI" id="CHEBI:136370"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:52093"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>9-(9Z-hexadecenoyloxy)-octadecanoate + H2O = (9Z)-hexadecenoate + 9-hydroxy-octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52068"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
            <dbReference type="ChEBI" id="CHEBI:136309"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:52069"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>9-(9Z-octadecenoyloxy)-octadecanoate + H2O = 9-hydroxy-octadecanoate + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52048"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:136282"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:52049"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>9-hexadecanoyloxy-octadecanoate + H2O = 9-hydroxy-octadecanoate + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52052"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83670"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:52053"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>9-octadecanoyloxy-octadecanoate + H2O = 9-hydroxy-octadecanoate + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:136286"/>
            <dbReference type="ChEBI" id="CHEBI:136373"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:52097"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a butanoate ester + H2O = an aliphatic alcohol + butanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47348"/>
            <dbReference type="ChEBI" id="CHEBI:2571"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17968"/>
            <dbReference type="ChEBI" id="CHEBI:50477"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:47349"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>a sterol ester + H2O = a sterol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10100"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15889"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:35915"/>
            <dbReference type="EC" id="3.1.1.13"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:10101"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:12045"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>an acetyl ester + H2O = an aliphatic alcohol + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12957"/>
            <dbReference type="ChEBI" id="CHEBI:2571"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:47622"/>
            <dbReference type="EC" id="3.1.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:12958"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>cholesteryl (9Z-octadecenoate) + H2O = cholesterol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33875"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16113"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:46898"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:33876"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="30">Interacts with CLC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 31">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="31"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRU7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012121"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012259.1">
        <property type="protein sequence ID" value="ENSXCOP00000012121.1"/>
        <property type="gene ID" value="ENSXCOG00000009102.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156231"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008126">
        <property type="term" value="F:acetylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004771">
        <property type="term" value="F:sterol ester esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00312">
        <property type="entry name" value="Esterase_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000100">
        <property type="entry name" value="Carboxylic ester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019826">
        <property type="entry name" value="Carboxylesterase_B_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051093">
        <property type="entry name" value="Neuroligin/BSAL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43903">
        <property type="entry name" value="NEUROLIGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00122">
        <property type="entry name" value="CARBOXYLESTERASE_B_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 31" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="32" id="KW-0472">Membrane</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="32" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="32" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="33">
        <location>
            <begin position="52"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00049290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00049322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00049428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00051791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00052473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00053019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00064516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="RuleBase" id="RU361235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="580" mass="63672" checksum="133EAD4CF74BAEAF" modified="2018-12-05" version="1">MCHVCTCVRCYLKSASLAIILVFRVLFLMAVLGVLVALSVLLEVISATSLGVVSTEGGLVQGQNLLLGFSRYMDVFKGIPFADIPGRFEKPKSHPGWSGTLKATQFRDKCLQLNPFMTIVSGNEDCLYLNIWVPHLGSVASNMPVMVWIYGGAFMFGESFGANFMGTHLYNGQELAERGNVIVVTLGYRVGTLGFLSAGDSTLPGNYGLWDQQAAIAWVHRNIQAFGGDPSNITVFGESAGGASVSFQTLTPHNKGLIRRAITQSGVALCPWSVNRNPRKYAEELALKVNCPTDSGMADCLKMTDPKVLTKAGTVSLTSSPDDPFVDNLALTPVIDGDFLPDDPSNLFHNTADIDYMAGANDMDGHIFTFYDVPSINSPLTFTSVDEIKRLLGAFTKEKGTLGFENAFSTYTLDWGSNPSQETIKKTAVAIETDYIFLIPTQAALYLHASNAGTGRTYSYLFSEPNRMGNYNKPWLGADHADDLQYVFGLPYSTPVMYWPKHRTLSGHMIAYWTNFAKTGDPNVGELRTPATWPTFTSTGHKFLEINSKMDAGYVGQKLRLRYVNFWTSIFPNLPSIYSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTB5</accession>
    <name>A0A3B5LTB5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Dual specificity tyrosine-phosphorylation-regulated kinase 1A</fullName>
            <ecNumber evidence="3">2.7.11.23</ecNumber>
            <ecNumber evidence="4">2.7.12.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Protein kinase minibrain homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:10217"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.23" evidence="3"/>
    <dbReference type="EC" id="2.7.12.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTB5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014070"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014245.1">
        <property type="protein sequence ID" value="ENSXCOP00000014070.1"/>
        <property type="gene ID" value="ENSXCOG00000010651.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157408"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14226">
        <property type="entry name" value="PKc_DYRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000087">
        <property type="entry name" value="Dual specificity tyrosine-phosphorylation-regulated kinase 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044131">
        <property type="entry name" value="PKc_DYR1A/1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF121">
        <property type="entry name" value="DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION-REGULATED KINASE 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="130"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="340"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="417"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="530"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="418"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="530"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="556"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="576"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="685" mass="75935" checksum="DD1E9287A79BEEC4" modified="2018-12-05" version="1">FSLHTAGLQMAAPMPHTHQQYSDRHQPSTDQSVTVLPYSDQTPQLTANQRHMPQCFRDPTSAPLRKLSIDLIKTYKHINEVYYAKKKRRHQQGQGEDSSHKKERKVFNDGYDDDNYDYIVKNGEKWMDRYEIDSLIGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEVRLLELMNKHDTEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNFRGVSLNLTRKFAQQLCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRSLLPFPRGAAGNVDQMNKIVEVLGIPPNHIMDLAPKARKFFEKLSDGTWSVKKTKDGKRYKPPASRKLHSILGVETGGPGGRRAGESGHAVADYLKFKDLILRMLDYDPKSRIQPYYALQHSFFKKTADEGTNTSSSVSTSPALEQSQSSGTTSSTSSSSGGSSGTSTSGRARSDPTHHHLHSGGHFGTTMPAMDGDSLCPQARQPYPPPLVWGGGVGPESVTGETHPVQETTFHVPPQHPKALHPHSHFHHHHGQMMATRPRPRHYTSPTHSSSTQDSMEVVHGHLSMTSLSSSASSSSTSSSSTGNHGNQAYQLRHLPAGALDFGQNGGLSMGLGAFSNPRQETGMAAHPAFSMGTNTGPGHYLAEGHLGMRQGMDREESPMTGVCVQQSSMASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTR7</accession>
    <name>A0A3B5LTR7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sodium/mannose cotransporter SLC5A10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Sodium/glucose cotransporter 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Solute carrier family 5 member 10</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Electrogenic Na+-coupled sugar symporter that actively transports D-mannose or D-fructose at the plasma membrane, with a Na+ to sugar coupling ratio of 1:1. Transporter activity is driven by a transmembrane Na+ electrochemical gradient set by the Na+/K+ pump. Exclusively recognizes sugar substrates having a pyranose ring with an axial hydroxyl group on carbon 2. Has likely evolved to enable renal reabsorption of D-mannose, an important constituent of oligosaccharide chains of glycoproteins. Contributes to dietary D-fructose reabsorption from glomerular filtrate across the brush border of the kidney.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>D-fructopyranose(out) + Na(+)(out) = D-fructopyranose(in) + Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72915"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:37714"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:72916"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>D-mannose(out) + Na(+)(out) = D-mannose(in) + Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72907"/>
            <dbReference type="ChEBI" id="CHEBI:4208"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:72908"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTR7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011214"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011345.1">
        <property type="protein sequence ID" value="ENSXCOP00000011214.1"/>
        <property type="gene ID" value="ENSXCOG00000008482.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159416"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005412">
        <property type="term" value="F:D-glucose:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018212">
        <property type="entry name" value="Na/solute_symporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819:SF128">
        <property type="entry name" value="SODIUM_MANNOSE COTRANSPORTER SLC5A10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11819">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00456">
        <property type="entry name" value="NA_SOLUT_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="261" mass="28494" checksum="806A1DF87E2A255A" modified="2018-12-05" version="1">SFLDISDIIVIATYFLLNLAVGIWSSCRVSRNTLSGYFLAGRDMAWWPIGSSLFASSEGSGLFIGLAGTGAAGGIAVAGFEWNATYVLLALAWIFVPVYISSGIVTMPEYLGRRFGGERIRTYLAVLSLLLSVFTKISTDLYSGALFVQVCLGWNLYLSTVLMLVVTALYTIAGGLAAVIYTDTLQTFIMIIGAIILTITGMCKFYHFKAEPPLRFSLRGGKLRLLTPCVFSRYQGNQRGGVTPGSRIELSVIQFLVERER</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUP5</accession>
    <name>A0A3B5LUP5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating per1 and per2, which may lead to their degradation. Participates in wnt signaling.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Monomer. Interacts with per1 and per2. Component of the circadian core oscillator.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 15">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUP5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013031"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013191.1">
        <property type="protein sequence ID" value="ENSXCOP00000013031.1"/>
        <property type="gene ID" value="ENSXCOG00000009869.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153536"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14125">
        <property type="entry name" value="STKc_CK1_delta_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000194">
        <property type="entry name" value="Casein kinase I isoform delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="9"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="301"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="301"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="404" mass="46024" checksum="97A12D3967A0FDAC" modified="2018-12-05" version="1">MELRVGNRYRLGRKIGSGSFGDIYLGTDISVGEEVAIKLECVKTKHPQLHIESKIYKMMQGGVGIPTIKWCGAEGDYNVMVMELLGPSLEDLFNFCSRKFSLKTVLLLADQMISRIEYIHSKNFIHRDVKPDNFLMGLGKKGNLVYIIDFGLAKKYRDARTHQHIPYRENKNLTGTARYASINTHLGIEQSRRDDLESLGYVLMYFNLGSLPWQGLKAATKRQKYERISEKKMSTPIEVLCKGYPSEFATYLNFCRSLRFDDKPDYSYLRQLFRNLFHRQGFSYDYVFDWNMLKFGANRAVEDAERERREREERLRHSRNPGARGMASASGRARAAQDAAAPSPLNPASHTGQSAVCMEKERKVSMRLHRGAPVNISSSDLTGRQDTSRMSTSQVLSSGARIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LW24</accession>
    <name>A0A3B5LW24_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LW24"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012039"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012177.1">
        <property type="protein sequence ID" value="ENSXCOP00000012039.1"/>
        <property type="gene ID" value="ENSXCOG00000009109.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287066"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="117" mass="12965" checksum="BBB869CB02C4310F" modified="2018-12-05" version="1">MHKWQAVGEHPKADVGDGEGDVVAHVLATGLLGVAGEPRLLVAPHLLGGGAEDEDAEDEQDSEPDLSDHSGVLLVQDTERHNLMHCGVGFVRNHYHVLICWRRFKKQVNDFIIHLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWA6</accession>
    <name>A0A3B5LWA6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Nuclear receptor coactivator 3</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SRC/p160 nuclear receptor coactivator family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015155"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015345.1">
        <property type="protein sequence ID" value="ENSXCOP00000015155.1"/>
        <property type="gene ID" value="ENSXCOG00000011471.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183021"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016922">
        <property type="term" value="F:nuclear receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061733">
        <property type="term" value="F:protein-lysine-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000008">
        <property type="entry name" value="Nuclear receptor coactivator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000008">
        <property type="entry name" value="Nuclear receptor coactivator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000027">
        <property type="entry name" value="Nuclear receptor coactivator 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056193">
        <property type="entry name" value="bHLH_NCOA1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010011">
        <property type="entry name" value="NCO_DUF1518"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032565">
        <property type="entry name" value="NCOA2/3_DUF4927"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009110">
        <property type="entry name" value="Nuc_rcpt_coact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014920">
        <property type="entry name" value="Nuc_rcpt_coact_Ncoa-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037077">
        <property type="entry name" value="Nuc_rcpt_coact_Ncoa_int_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017426">
        <property type="entry name" value="Nuclear_rcpt_coactivator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014935">
        <property type="entry name" value="SRC/p160_LXXLL"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10684">
        <property type="entry name" value="NUCLEAR RECEPTOR COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10684:SF3">
        <property type="entry name" value="NUCLEAR RECEPTOR COACTIVATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23172">
        <property type="entry name" value="bHLH_NCOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16279">
        <property type="entry name" value="DUF4927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16665">
        <property type="entry name" value="NCOA_u2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08815">
        <property type="entry name" value="Nuc_rec_co-act"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14598">
        <property type="entry name" value="PAS_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08832">
        <property type="entry name" value="SRC-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01151">
        <property type="entry name" value="DUF1518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69125">
        <property type="entry name" value="Nuclear receptor coactivator interlocking domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BHLH" evidence="18">
        <location>
            <begin position="23"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="17">
        <location>
            <begin position="115"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="462"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="561"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="646"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="749"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="918"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="462"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="562"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="612"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="666"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="697"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="763"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="783"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="808"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="820"/>
            <end position="836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1447" mass="153388" checksum="A6619A11CFF906E1" modified="2018-12-05" version="1">MSGLGDNSMEPLSSENRKRKLSTCDTSGLGCERKRREQESKYIEELAELISANLSDIDSFNVKPDKCAILKETVRQIRQIKEQGKASSNDDDVQKSDVSSTGQGVIDKDHLGPLLLQALDGFLFVVNREGSIVFVSDNVTQYLQYKQEELINTSVYNILHEEDREEFHKNLPRTNINGVSWGNEATRQKSHTFTCRMLVKFGHAHGPMEEGPGGPRYETMQCFALTQPKAMIEEGEDLQSCMICVARRVTAVERTESFNTRHELSGKLIQIDQSSLRASMRPGWEDLLRRCIQMFLQHSDGQPWSHKRHYHEAFLQGHAETPLYRFSLSDGTPVTAQTKSKLYRHPMSNEPQGFISTHLLQREPNGYRSAPGGNMMPNNMRHQGMGNANMNPQMNVNAGGGMGMGGMGGMNRGFGMSEQSHMGHMGPMGGLGSRMMQMNQMNQMNQVGHMNQMSQMGPMNHPGPGMQQHPQQQPPFQSAGGFGLSGMNSPSGSPRMGGPQPGLLMSPRNRGSPKMGANQFSPGGMHSPMSGLGGGGAAGGNTCTFSSSSLNALQAISEGVGSSLPSTLTSPSAAHKPDSSPSIHSSSQPSHQAKPGQDGSKSPAGAAKECGEASGEAGMAGADPPRRLPDSKGHKKLLQLLTSPTEELGISGSGPTGPAGPPPPNSSGASAGSDSKEATGGMTSPSSTGVSSSSASGGAGLAPGQGGASASAHYTGSLQEKHKILHKLLQNGNTPDEVAKITAEATGKVTSAAGSGPGLIADPKQEQHSPKKEKTHALLHYLLNKDDSKEQTDVKPKLEDLEGKCLPGAGGGGGGGPAAEHAESKIKSEPPDDLHNLESILGDLRGSSSDFYPDQTGGAGANNTGNKPQGCLDDGLQVMGPGPRGPFQRTMSLDGKPLGGPGGAVRRPMLIKQENMMGRPMNRGAGPQRSPMGGSGDWKPRSSISPVSSAGHPSMMRPGMEYNNGKSVMSGPMVSRSNSVPGNRSMLQQQLMDMGSSDMGMGMSPFSQQGQPSQSPSWHEPMMNMEGNRWFGNPLDDLLVPPTTSEGQSDERALLDQLDSLLNNTDGIALEEIDRALGIPDLVSQVRCQKEPHCSVHFGGQDPSMLLDQKPLYGAGYPGPPAMPMQSGYGGNPMQGQGQQAGFGPMLSPMGQGGSFPGMGGMGGMGHPRANMMRPRMMNANKPMRLQLQQRLQGQQFMNQTRQGMAMKMENPGGNPGMRPGMQPGMGGQQGFLNAQMMVQRNRDVVNLHMRRQRMMMLMQQQQQQAAAAAAAAAGGFSPPPNVTAPGGMDNPMGGPNMGQPGPQQFGYGGSYGVTQQGDPSFGPSGGSPPNAMMPGRMGPQNPMMQQHSQGGPMYPGADMKGWSQGGMGRNRYDQQFGQQGNPGQYGGMMMNGGMPASGGGGHMGQMGMNPMVMGRMPMGPDQVCAKSGSLNTERTLIVYSEDGLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWS1</accession>
    <name>A0A3B5LWS1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcription factor 25 (basic helix-loop-helix)</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
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            <authorList>
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        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT TCF25"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="387" mass="44665" checksum="9FD7C817E78C2B4F" modified="2018-12-05" version="1">MQRLSQKSSEIHVSHYLSYCTLTRVALLQLNPYHIDSLLQLSDVCRIQEDQEMARDLIERALYSFECAFHPLFSLTSGTSQLDYLRPENRAFYLALYKHMMFLEKRGCPRTALEYCKLILCLDPDSDPLCMLLLIDFLMLRSREYKPLLQLYQDWEEHRNLSQLPNFAFSCALCHFHLSQQDDVDPEESDKQRQQADQMLQSALIMFPGVLMPLLDLCTVQPDSTVSSHSFFGPKSQIGQPPALAELTALYVGRTYSLWKEAVVMLWLEESVKEVLRRVDAKDPLVEDCENKRKQRYQSAPRNIHRHVLLSEIKEATSTLPLEVTIQPVMGFDPLPPLDSVISYTRPESLTYAFLKKNILCHCRMLPPPCFTIAAVCSGRWAVFVFC</sequence>
</entry>
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    <accession>A0A3B5LWS5</accession>
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            <fullName evidence="1">PID domain-containing protein</fullName>
        </recommendedName>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
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            <dbReference type="Ensembl" id="ENSXCOP00000013781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="157" mass="17341" checksum="3F646F0CA465A8B4" modified="2018-12-05" version="1">MTWKENFTLSFQQVAKTVKRKTSVCLYTETCPAPSSPSVIEEDSVQFNKLSYLGCTWVKAPRNEAEAQSAMATLRAESAIPIPITLHVPCGPDGSVRIVDQASGTEIASFPIYKVLFCARGREGSVESDCFSFTESYRSSEDFQIHVFSCHIKEAVS</sequence>
</entry>
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    <accession>A0A3B5LWT1</accession>
    <name>A0A3B5LWT1_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <begin position="28"/>
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            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="235"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="250" mass="26378" checksum="94A36B7AB278A4D5" modified="2018-12-05" version="1">MDSNNHFNYGTSTNSGLKLSSGDSLYTNGSSTSFPQQGKNLNGEMNVNGVTTVHGSSVSGSHPPSAPYPHMSGHHQGAVGYEYLWGGLPQYGSGMGPSSGQGVHQKQPASGIAQSQSQHHFQSHGQYQLNGGVEASHQPPIASQTNQHYSMMTNGMPFYQPPQQQLPQQSQSQTRMMPPAAQNFTPPRGSPQHHSLGRGSCSSPLPVGMSPTPVVSPPIQDSGSPKGLSRDRSPHTSSAGMSSPLQGDFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LX99</accession>
    <name>A0A3B5LX99_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cleavage stimulation factor subunit 3</fullName>
        </recommendedName>
        <alternativeName>
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        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Cleavage stimulation factor 77 kDa subunit</fullName>
        </alternativeName>
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        <name type="scientific">Xiphophorus couchianus</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF3 directly interacts with CSTF1 and CSTF2. Interacts with FIP1L1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cleavage stimulation factor subunit 3"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HAT"/>
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        <property type="entry name" value="Rna14-like"/>
    </dbReference>
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        <property type="entry name" value="Suf"/>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 3"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR19980">
        <property type="entry name" value="RNA CLEAVAGE STIMULATION FACTOR"/>
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    </dbReference>
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        <property type="match status" value="9"/>
    </dbReference>
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        <property type="entry name" value="TPR-like"/>
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    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
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            <begin position="1"/>
            <end position="449"/>
        </location>
    </feature>
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        </location>
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            <begin position="478"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00055756"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00062648"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00074328"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00076953"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00078012"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF05843"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="621" mass="71635" checksum="6BA7A624F31C82E0" modified="2018-12-05" version="1">MKVLHIDLWKCYLAYVRETKGKLPSYKEKMAQAYDFALDKIGMEIMSYQIWVDYINFLKGVEAVGSYAENQRITAVRRVYQRGCVNPMINIEQLWRDYSKYEEGINVHLAKKMIEDRSRDYMNARRVAKEYETVMKGLDRNAPSVPPQNSPQEAQQVEMWKKYIQWEKSNPLRTEDQTLITKRVMFAYEQCLLVLGHHPDIWYEAAQYLEQSSKLLAEKGDMNNSKLFSDEAANIYERAIGTLLKKNMLLYFAFADYEESRMKYEKVHSIYNKLLAIEDIDPTLVYIQYMKFARRAEGIKSGRAIFKKAREDLRTRHHVYVSAALMEYYCSKDKSVAFKIFELGLKKYGDIPEYILAYIDYLSHLNEDNNTRVLFERVLTSGSLSPEKSGEIWARFLAFESNIGDLASILKVERRRFTAFKDEYEGKETALLVDRYKFMDLYPCSTSELKALGYKDVSRAKLAAMLPEAVVAPSVPTLKDDPDRKPEYPKPDTNQMIPYQPRHLARLHPVPGGVFPVPPAAVVLMKLLPPPTCFTGPFVQVDELMETFRRCTLPESVDAAVELITGRQPEAAGEGNGPMENHATAKSLKRPNADSDEEDDKGAIAPPIHDIYRARQQKRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYB2</accession>
    <name>A0A3B5LYB2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial pyruvate carrier</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the uptake of pyruvate into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYB2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015910"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016107.1">
        <property type="protein sequence ID" value="ENSXCOP00000015910.1"/>
        <property type="gene ID" value="ENSXCOG00000011827.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000046988"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006850">
        <property type="term" value="P:pyruvate import into mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005336">
        <property type="entry name" value="MPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03650">
        <property type="entry name" value="MPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="108" mass="12507" checksum="C7A8F0AB06D2A42C" modified="2018-12-05" version="1">MAGTIARKAIDHLKSKEFRDYLTRFHFWGPVANWGLPIAAITDMKKSPEIISGRMTFALCCYSLLFMRFAYKVQPRNWLLFACHLTNESAQLVQGSRLIKYNMEKKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYS2</accession>
    <name>A0A3B5LYS2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYS2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012934"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013094.1">
        <property type="protein sequence ID" value="ENSXCOP00000012934.1"/>
        <property type="gene ID" value="ENSXCOG00000009792.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159191"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF8">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="9">
        <location>
            <begin position="119"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="314"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="377"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="337"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="377"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="420"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="474" mass="52278" checksum="CD8F3E9C8D946ACB" modified="2018-12-05" version="1">MEVCGRNPNRTAPTPDPRRSEVPLCSRTNGWTWPPHPFQLLAWLLYVYFAVTGFGVFVPLLPPHWVPAGYICTGILFVSHLCVHVLAVSIDPADLNVRMRSDRGPVPAFDRSKHAHVIENCHCYLCQVDVGPKSKHCSACNKCVANFDHHCRWLNNCVGSRNYKLFLHSVVSALLGVSLVLVFASYVFVEFFRDPSNLRTDKYFLVQNQTGVWFVFLPVAPLRSAAAVIPVLAAITMVMGLLSSGLLCHLLCFHIYLMWNRLSTYEYIVRQRHRLDSRDPRTAAAAKQSRRPSVVQDVNYSGTLGYTNPQLDVQQEQAETSESSQGQRLPFPAFPLRAPPGHAPAPGPIQAAAPPADYHSDSAESLEEIPVALAKLGSSSSGDAAGTSRRADRRLARGARGPLPDRPAPALHKTRKKNPSSRGNRTTAQLRFEMASTQPPSVFVSRTSGETAEVLGWTYTPTEPSGVKGQQHND</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0H3</accession>
    <name>A0A3B5M0H3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutaminyl-peptide cyclotransferase</fullName>
            <ecNumber evidence="4">2.3.2.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Glutaminyl cyclase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Glutaminyl-tRNA cyclotransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-terminal L-glutaminyl-[peptide] = N-terminal 5-oxo-L-prolyl-[peptide] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23652"/>
            <dbReference type="Rhea" id="RHEA-COMP:11736"/>
            <dbReference type="Rhea" id="RHEA-COMP:11846"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:64722"/>
            <dbReference type="ChEBI" id="CHEBI:87215"/>
            <dbReference type="EC" id="2.3.2.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glutaminyl-peptide cyclotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.5" evidence="4"/>
    <dbReference type="RefSeq" id="XP_027854875.1">
        <property type="nucleotide sequence ID" value="XM_027999074.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027854876.1">
        <property type="nucleotide sequence ID" value="XM_027999075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0H3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016760"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016977.1">
        <property type="protein sequence ID" value="ENSXCOP00000016760.1"/>
        <property type="gene ID" value="ENSXCOG00000012639.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114133289"/>
    <dbReference type="KEGG" id="xco:114133289"/>
    <dbReference type="CTD" id="561142"/>
    <dbReference type="GeneTree" id="ENSGT00390000003107"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016603">
        <property type="term" value="F:glutaminyl-peptide cyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03880">
        <property type="entry name" value="M28_QC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000029">
        <property type="entry name" value="Glutaminyl-peptide cyclotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037457">
        <property type="entry name" value="M28_QC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040234">
        <property type="entry name" value="QC/QCL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12283:SF3">
        <property type="entry name" value="GLUTAMINYL-PEPTIDE CYCLOTRANSFERASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="16">
        <location>
            <begin position="155"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="394" mass="43430" checksum="45CED8D9C6A4E9A1" modified="2018-12-05" version="1">MSRSSRRYNKPPPQSNGGSLPGCDRVRIPGARVLLLGLLGVLVLAVVLSVYLSNDTSGGHGSRLPAVDLVKDRLSHKPSKVSVAQIRRLASLVDGARLWETHLRPILIERLPGTRGSLAVQQHITSSLSSLSAGWSVDLDSFRSPTPRGQVTFTNVVAVLDPAAPRRLLLACHYDSKALPPDPRAPERVFLGASDSAVPCAMILELATSLDAQLRSFKQQKLPVTLQLVFFDGEESFEEWTATDSLYGSRHLAELMGNTPHPAASSRTTALQAVDLFVLLDLLGGPDPLIANHFDNTARWFDRLISAEKRLHRQGLLLSHPAEQTYFRKDVYLGPVQDDHIPFLHKGVPVLHIIATPFPQFWHTLDDTEENMHRPTVENLTKIMAVFLAEYLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0V3</accession>
    <name>A0A3B5M0V3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Family with sequence similarity 124B</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM124 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0V3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017032"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017252.1">
        <property type="protein sequence ID" value="ENSXCOP00000017032.1"/>
        <property type="gene ID" value="ENSXCOG00000012850.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00590000083134"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029380">
        <property type="entry name" value="FAM124"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046365">
        <property type="entry name" value="FAM124_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14715">
        <property type="entry name" value="FAM124 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14715:SF2">
        <property type="entry name" value="PROTEIN FAM124B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15067">
        <property type="entry name" value="FAM124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM124" evidence="3">
        <location>
            <begin position="12"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="283"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="435" mass="48083" checksum="D64E791EE9698825" modified="2018-12-05" version="1">FEFRQQQLLPINLHLLSNPGDSLLLQQTLDRLLRWLCPSLRIFHVSERASPFRAHTHLHYPSLAITFFLHEAYGEERILKVLDFFQRPPWHSSPSNALLRPYLLPSRDFYSLGPCMPVWGVRPVHCGGEILRITLYSSYDNYEDAVRFYETILQRQAEEQKAGFCWFTLHAEPGLCLQLALKQLSPGVGVEACSSAVLQFSVEEVGQLVPLLPNPCAPISNTRWQTEDLDGNKVLFQVSLREIMCLKVEPGLEKLLGGCGGAESLGSGSCCSTPPGSSCYSSQRSSPAQLSTSNHPDSPLRPSVTRSLSYLLLEEEEEETNVDTGVAISPLSDTAVATITRSSSCDLLRTRNSAKPSVVGASPVEQRAKELTKCLLQTHINHQAPSGGCKGAAEESKTTARGQRTSQKLLAENSTSTELLSAYINHEEPVDEFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M1L3</accession>
    <name>A0A3B5M1L3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4939 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M1L3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013914"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014084.1">
        <property type="protein sequence ID" value="ENSXCOP00000013914.1"/>
        <property type="gene ID" value="ENSXCOG00000010536.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000173342"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032549">
        <property type="entry name" value="DUF4939"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16297">
        <property type="entry name" value="DUF4939"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4939" evidence="2">
        <location>
            <begin position="86"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="176" mass="20081" checksum="C07673FD4B83047B" modified="2018-12-05" version="1">MTEHSGQNPSPADAIRKTLTEQHALLQSHESALQELNTRQIETNRWLNELMNFLQNSVPQSPAPEPTPAPDPDPPVRSTFSEIHPPTPERFSGDLDKCKGFILQCSIIFNHSPQRFVHDSAKIAYVLSLLSGQALSWAEARFPSPTDYDCTFEEFLREFKQVFSHDHDKIFHSREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M1W7</accession>
    <name>A0A3B5M1W7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Metalloendopeptidase</fullName>
            <ecNumber evidence="13">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12 13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12 13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Zymogen granule</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M1W7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014193.1">
        <property type="protein sequence ID" value="ENSXCOP00000014019.1"/>
        <property type="gene ID" value="ENSXCOG00000010612.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154856"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042588">
        <property type="term" value="C:zymogen granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000040">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF779">
        <property type="entry name" value="METALLOENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 12 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12 13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 12 13" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017101118" description="Metalloendopeptidase" evidence="13">
        <location>
            <begin position="22"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12A" evidence="14">
        <location>
            <begin position="52"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="249" mass="28629" checksum="AB55D0D7B0061569" modified="2018-12-05" version="1">NYFTIITVCTVLICLVEHLKSGLLNTLNVWMHTEDEPLVIDDIAYKNENERNADQCTSRGCLWPKSADGKVYIPYMIANHYTSRELSVIERGLQSFSHVSCIRFTRRSSERDYLSIQSHNGCYSYVGRLGYSQTLSLDQAGCIYHSTVQHELLHALGFNHEQCRSDRDQYIRILWENIQSGLAYAFDKLGTLNLNTPYDYNSVMQYHRYAFSANNQPTMVPIPDSSVSFGTATEMSMNDITRLNRLYNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M629</accession>
    <name>A0A3B5M629_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein naked cuticle homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Cell autonomous antagonist of the canonical Wnt signaling pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the NKD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M629"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016861"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017078.1">
        <property type="protein sequence ID" value="ENSXCOP00000016861.1"/>
        <property type="gene ID" value="ENSXCOG00000012717.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000033589"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060971">
        <property type="term" value="P:embryonic heart tube left/right pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003146">
        <property type="term" value="P:heart jogging"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070121">
        <property type="term" value="P:Kupffer's vesicle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042308">
        <property type="term" value="P:negative regulation of protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001839">
        <property type="term" value="P:neural plate morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060061">
        <property type="term" value="P:Spemann organizer formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000166">
        <property type="entry name" value="Naked cuticle homolog 1 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040140">
        <property type="entry name" value="Nkd-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22611">
        <property type="entry name" value="PROTEIN NAKED CUTICLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22611:SF2">
        <property type="entry name" value="PROTEIN NAKED CUTICLE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="EF-hand" evidence="12">
        <location>
            <begin position="118"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="256"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="415"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="320"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="416"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="434" mass="48953" checksum="6B63A723C642384D" modified="2018-12-05" version="1">MGKLHSKHGDSFVVNACLAKKGVDDWLVKQKYYCTSSRLEQQDCHHKSNCGLTARDSLVDACGERIIDEHYRLEVALPPEKTDSCCVEEKMQERDSRSPAGPQKQLQFEELECAVSVEEDNRQEWTFTLYDFDNNGKVTREDITSLLHTIYEVVDASVNHSPGNSKTLRVKLSVAPDSSQRWRSCAQGTTGKCHKNDKCIDDLKSADKKSRALLRRHHSDHHTQSGCQRHCVDENLERRNHYLDLAGIENYTSRFGAGPAAEPTKVEAQARSSNQARSRSHEPENGHPHSHHRRLQSAVDTPAAESLHPARPRGQDSGRHGIRSPKTHSRTPPAQNSGRVMRSRGAPPPPALPSQTTPHQSAAPYRKHKQRSKESQGYRALGSLGSPGPIVEKEQVRDLPSVLLYEGGLAQVVQRHEHHHHHEHHHHYHHFYQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6S8</accession>
    <name>A0A3B5M6S8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Si:dkey-283j8.1</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM255 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6S8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017051"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017271.1">
        <property type="protein sequence ID" value="ENSXCOP00000017051.1"/>
        <property type="gene ID" value="ENSXCOG00000012864.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160889"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028014">
        <property type="entry name" value="TMEM255"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33721:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 255B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33721">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 255B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14967">
        <property type="entry name" value="FAM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="209" mass="23150" checksum="D1199C8C24E6C618" modified="2018-12-05" version="1">VRRALWLVLSMLSLSLLLVVLGAYTTTRTESLNVTGYISGVFLTAAIVFLSFGIITSFLCLMIDGVGILFNMDVRPLKAGRCQYYSSGSKKYYSHLVPCQNLRESCTMTVRSGTCYCCDLYNCANGDYMNNYFEFVGVQSCEEVFTLYILIWTLTGVNLVAFFTGILTAAVLGNIKYLRFVPTVLSESNPPPFNPLTTLLSQRVHGLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7A8</accession>
    <name>A0A3B5M7A8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">G protein subunit beta 1 like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">GNB1L</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027888962.1">
        <property type="nucleotide sequence ID" value="XM_028033161.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSXCOP00000020143.1"/>
        <property type="gene ID" value="ENSXCOG00000015096.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
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        <property type="entry name" value="WD40_rpt"/>
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    <dbReference type="PANTHER" id="PTHR19854:SF1">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT BETA-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TRANSDUCIN BETA-LIKE 3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="WD40"/>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
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        <property type="entry name" value="WD40 repeat-like"/>
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        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_REGION"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
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    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="283"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="328" mass="36032" checksum="D1E081689F47A3A3" modified="2018-12-05" version="1">MSRPAPCPVYTLRGAGAPLNTLHFRCSGGDSPLLFSGSAKGAVHIWNLKSRRAERVLEGHGSNSVIWVSTLQTADTLISQGRDMRVCLWDLTEGRIEALDSVWTGSVGFCQCSVLETSPSSYLLAFAGPQTEEIKIIEMPSKNPVCTLTPDTKLGMVMCVQLWKPDSGLGPLLLAGYEDGSLLLWDVTQRSELSRVKAHPEPVMCLTFDPGRLRGVSGSCEKKLSSWMLDGQNHIQLQDCVTLVNPGISQLCVRSDGRLLASAGWDHRVRVFGWKKLQPLAVLHYHTDMALSVAFSDHQDPKQRLLAAGSKDQRISLWSIYNQNADTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7I5</accession>
    <name>A0A3B5M7I5_9TELE</name>
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        <recommendedName>
            <fullName evidence="9">Period circadian protein homolog 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Circadian clock protein PERIOD 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
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        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="ENSXCOG00000014612.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0001222">
        <property type="term" value="F:transcription corepressor binding"/>
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        <property type="term" value="P:circadian regulation of gene expression"/>
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        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045475">
        <property type="term" value="P:locomotor rhythm"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:negative regulation of angiogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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    </dbReference>
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        <property type="term" value="P:positive regulation of circadian rhythm"/>
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    <dbReference type="GO" id="GO:0009409">
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:response to hydrogen peroxide"/>
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    <dbReference type="GO" id="GO:0009411">
        <property type="term" value="P:response to UV"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="Period circadian protein homolog 3"/>
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        <property type="match status" value="2"/>
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    <dbReference type="InterPro" id="IPR050760">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11269">
        <property type="entry name" value="PERIOD CIRCADIAN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PERIOD CIRCADIAN PROTEIN HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="6" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PAS" evidence="12">
        <location>
            <begin position="414"/>
            <end position="457"/>
        </location>
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            <begin position="1"/>
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        <location>
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        </location>
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        <location>
            <begin position="859"/>
            <end position="870"/>
        </location>
    </feature>
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        <location>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1375" mass="151214" checksum="DF65A969B7ED9E5D" modified="2018-12-05" version="1">MSDDSDSKPYPLPVPADRDRASASSSPMAQMRWVGSYAQGRPDLGLPSEGSDSSGQDPAISPHNVRKTTRSRLSPEEDVEMKSSGSSGSGTESHGNESHGNESHGNDSNGHESMGSSTSRSKDSALLESLGSNKSSNSHSPSPPSSSNAYSLLSSEQDNPSTSGCSSQESAKAKNQKEVIKTLKELKLHVPADKQHNSKSTTLSALKYALRCVKQVEANEEYYQMMMVNDSHPSGLDVSSYTIEEIDSITSEYTLKNNDIFAVAVSLSTGKIIYISDQAASILNCKRDAFKDSKFVEFLMPQDISVFYSFTTPYRLPSWSMCTGADPSPSDCMQEKSFFCRISGGNGFEGDLRYYPFRMTPYLMKVQDMAHDEDQFCCLLLAERVHSGYEAPRIPPDKRIFTTTHTPNCVFQDVDGRAVPLLGYLPQDLIGTPILFHLHPNDRPVMLAIHKKIIQCAGQPFDHSSIRFCAQNGEYIVLDTSWSTFVNPWSRKVSFVIGRHKVRMGPMNEDIFMAPVSCTGHMKNTDSDIQEITEQIHRLLLQPVHNSGSSGYSSLNSNEYLQGMTSSSSESLNNGKQGKKQEKEGEQSSKSRPRTFQEICKGIHLQKNQEHDCHIYDFIQKLIFDSAPPINWREATAADSRLSFHEELDLNDQTAYSYQQISCLDSVIRYLESYNVPVLMKRKCQSSSNTTSSNSDEDKQKKSHTVQISEEPTLSKDQSRISALDDPHKKTSDPATAVVGTSMPLPVPNKPESVVSITSQCSYSSTIVHVGDKKPQPESGTNINTPRPVSPPSQEMESYKKLGLTKQVLAAHTQKEEQTFLCRIRELRGLAPHKANGSPPPDQQRDERTATAAHTVQPRKQEGQRPERTPRRGTRNKKTKTKRAKLLTSPSTEAAPHKERQPQQPPPQPQTNQSHMQIPVSPSDASQPVFPAPYPVMLPSYPMQIYARTDSALLRTEAPPQDLVNNQGAQLPLLFPGTQSVPVTSHMVTPIMAFVLPNYLYPLIGHQAPPVPVYQVDAGGVPMQAQPFDQTAFSSQAAFATPPFINSQNQLNPMLASYLSPPFYFPSSNDTPKPPAEGRSRSSTPQSGESEGQPSPPLFQSRCSSPLNLLELEFSVDRQDNAPHASVGQGSNMAEREKGASASKRSDEGNNSDVISTSSDMLDIILQEDSCSATSGSMGSGSNGFGTSASRMSKSRTSASRSNNSSKYFGSVDSSQSSQKHKGLLRSSEERPAETGLLKCVLQDPVWLLKANTDEKVMMTYELPSRDIDRVLEEDNEKIRVLQQCQPHFSVDQKKELCEVHPWIEKGELPKAINTAATVGAEDQPDLGLGDTETGEDCCPERLRGEPLTSAMVSCHSGPPSSCSPRAEEANQQEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9H5</accession>
    <name>A0A3B5M9H5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9H5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020192"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020439.1">
        <property type="protein sequence ID" value="ENSXCOP00000020192.1"/>
        <property type="gene ID" value="ENSXCOG00000015136.1"/>
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    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="57" mass="6619" checksum="7DB84908A73B90D7" modified="2018-12-05" version="1">MEGNSPLPLFLLSTLICVFIVTGAHHLFPFHSPIPIDTRHHEGRYHYEPHPLHAMHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9M8</accession>
    <name>A0A3B5M9M8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">POC1 centriolar protein homolog A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">POC1A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
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            <taxon>Metazoa</taxon>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Atherinomorphae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <text evidence="4">Belongs to the WD repeat POC1 family.</text>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:multicellular organismal-level homeostasis"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WD_REPEATS_REGION"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="15"/>
            <end position="56"/>
        </location>
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        </location>
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    </feature>
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            <begin position="321"/>
            <end position="334"/>
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    </feature>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00039725"/>
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    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017991.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="413" mass="45937" checksum="ECA412C12E5ABD14" modified="2018-12-05" version="1">TVSALDSDPTLERHFKGHRHTVTSVDFSCNMKQIATGSVDSCVMIWNMKPQMRAYRFDGHKEAVTCVQFSPSGHLVASTSRDKTIRLWVPSMKADSTVFRAHTATVRCVNFSGDGQTMLTSSDDKTIKLWTVHRQKFLFSFTQHMNWVRCAKFSPDDGLIVSCSDDKTIKLWDKKSRECVHTFTEHAGYASYVDFHPSGTCIAAASTDTSVKIWDIRTHKMLQHYPVHSGAVNSLSFHPGGNFLITASSDSTMKILDLVEGKLLYTLHGHQGAVTCVAFSRAGDYFSSGGSDEQVKVYSFGHSDGHSRGTHSHAHRDAATRSPSTQQTQGSQLPAHQDKGLSDVSDGRVPSSLANTLEHIIGQLDVLTQTVSILEQRLTLTEDKLKECLENQMEIGLHLQNRDEGRRWPNQHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9Q2</accession>
    <name>A0A3B5M9Q2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma</fullName>
            <ecNumber evidence="13">2.7.1.149</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phosphatidylinositol 5-phosphate 4-kinase type II gamma</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + ATP = 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83423"/>
            <dbReference type="ChEBI" id="CHEBI:84968"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:55993"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + GTP = 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:83423"/>
            <dbReference type="ChEBI" id="CHEBI:84968"/>
        </reaction>
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            <dbReference type="Rhea" id="RHEA:55965"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-5-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12280"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57795"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
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        </reaction>
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            <dbReference type="Rhea" id="RHEA:12281"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="Ensembl" id="ENSXCOT00000021246.1">
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        <property type="gene ID" value="ENSXCOG00000015670.1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
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    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
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    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF35">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 5-PHOSPHATE 4-KINASE TYPE-2 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="18">
        <location>
            <begin position="39"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="292"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="294"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="419" mass="47541" checksum="1F07A7A319005201" modified="2018-12-05" version="1">MAALGNSGTVTSSPMVVLAPKTKTKKKHFVQQKVKVFRASEPIVSVFIWGVNHSINDLSQVPVPVMLLPDDFKANTKVKVNNHLFNKENLPGHFKFKEYCPQVFRNLRERFGIEDLDYQVSLTRSPPVRSADDQGESLLLNSYDRTLVIKQISSEDVADMHNILSEYHQHIVKCHGSTLLPQFLGMYRVSVDSEEAYLIVMRNMFSHRLVVHRKYDLKGSLVDREASDKEREKELPTFKDMDFRNNLQKVYVTDEEKEKFMEKLNKDVEFLVKLKIMDYSLLLGIHDIGRTEREEEESGEVSNEEEAESENGLAPGPAVGSYGTSPEGIAGYMSSCKPLGPGEFDPYVDVYALRSCPGEKFGREVYFMGLIDVLTQYDTKKKAAHAAKTVKHGAGAEISTVHPEQYAKRFRDFISKIFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MA60</accession>
    <name>A0A3B5MA60_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bridge-like lipid transfer protein family member 3B</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSXCOT00000018392.1">
        <property type="protein sequence ID" value="ENSXCOP00000018161.1"/>
        <property type="gene ID" value="ENSXCOG00000013687.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00600000084428"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026728">
        <property type="entry name" value="BLTP3A/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22774:SF17">
        <property type="entry name" value="BRIDGE-LIKE LIPID TRANSFER PROTEIN FAMILY MEMBER 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22774">
        <property type="entry name" value="CHOREIN N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24917">
        <property type="entry name" value="BLTP3A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="264"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="749"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="863"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="934"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1100"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1307"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1335"/>
            <end position="1355"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1361"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="276"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="405"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="944"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="965"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1123"/>
            <end position="1132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1397" mass="153255" checksum="6F98E065A50F70E8" modified="2018-12-05" version="1">LASLVLPQLLVLFAKNLSPDKINLSTLKGEGQLTNLELDEEVLQSLLDLPTWLAINRVFCNKAAIRIPWTKLKTHPISLTLDKVIMEMSTCDEPRPPNGPSPIATASGQSEYGFAEKVVEGISLSINSIVIKISAKAFNASFELSQLQVYSVNTSWSISDLRFTRIQDPQRGEILTFKEISWQMIRIEADAIQSAEHEMLSAPIRLITNQSKIRVTLKRRMKDCNVVASKLILVLDDLLWVLTDSQLKAMVQYAKSLSEAMEKSAQQRKSMAADDQASSPPQTAQQVRTQQASTAADQSATMAKLFSAYDVCETSHHLQITHLDLHICDDIHAKDKGGAMQLSFSSITLDYYPFHKAGRFFASFCVHWMHYSEATKTRETWAKSLLDEYKTDVERLKGAVRDRQGPGAAHGSPHHGSPPPPPKTQLMSSSFVLRMADFSIYQVSMADQRRSSPKTMISCNKKSLYLPAEMPAIHVEFTEYYFPDGKDYPIPCPNLYAQLNALQLVLDPRSLVWINLFALDLRQSLEQFMQIYKLNDSQKPDEHVDIKVDGLMLKLVVPTEPDASYAADLPRSVSIQTSEMVATNTRYPPNCTRSHLEALLQAFEGEPFFSPSFSTFPRSPSSVPVLHPVFQQHAHEQDTKLQHIHRGLVEPTMGADALKMPAAADFWALHFAQFWVDYEGSRGGKGRPQPFVDSFPLTVWACQPTKLVQHQEKLKDSVGTGLSRSSSAEAVARLQRKRILKEYYSADSAPASTQSNGLHKPHSLDSVPNSRCSLSPSNDADVHLLVHVQKPLSAQVSHRQYVFLMHLLRSLKTLQQTLQQDLESMASKRERKDAMHRPADHKPFTFCLGLLLKSAEVSLLMKPIPQPEGSGSPAGSELSPSESRGTLEPAGDVAEGPGKANEEPGSQDGGAAKQPCTVDQLLCGEGPEGGATLYATPLIPSSTCEERTPVKNPDRGASEAVGEGLVDGLGTGDEAGSGLDPKTALTDPLSDFSDKEKAAAAKMPQSISRKGSLSVVSDLLSSSNSSRSTSLYSMSNIGRLMRDRSQSSFSVSYKNMKKSPSLQSLDNISIDSYLMEDGDTYSLTERDDVSISGFKDAISEQSVPESAAEAVTVQEKDGAASPDSVSAASQSIDEPAKDTVSVLVLKVQTLCVGMEAVGESSAVTLEVGRVTPNQLGNIGLREYLSNRSLAGGETRSGSSRGLYNPEVRARLESGPSAATHSPLAERNGFLQLHLHGYQARFMMASLRNLALFLEGDRSTDVMPMEIRIRDTHINLEARKPYDSADNPDAETNTLHVDSLVVRRNDDGSFSVGGKRTQTDSTLSPLPEAVSGVCRTAKATQTQTPPSSPRPTDREKMLLEENECLKVELSRAKMALAEAQMEKDSLLHRMKSLKMNTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MB11</accession>
    <name>A0A3B5MB11_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sperm surface protein Sp17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Sperm autoantigenic protein 17</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Sperm surface zona pellucida binding protein. Helps to bind spermatozoa to the zona pellucida with high affinity. Might function in binding zona pellucida and carbohydrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer. May interact with ROPN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000016894"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017111.1">
        <property type="protein sequence ID" value="ENSXCOP00000016894.1"/>
        <property type="gene ID" value="ENSXCOG00000012739.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000039164"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007338">
        <property type="term" value="P:single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12100">
        <property type="entry name" value="DD_CABYR_SP17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.890.10:FF:000006">
        <property type="entry name" value="Sperm surface protein Sp17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003117">
        <property type="entry name" value="cAMP_dep_PK_reg_su_I/II_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047579">
        <property type="entry name" value="DD_CABYR_SP17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10699">
        <property type="entry name" value="NEUROMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10699:SF16">
        <property type="entry name" value="SPERM SURFACE PROTEIN SP17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02197">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00394">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47391">
        <property type="entry name" value="Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="RIIa" evidence="9">
        <location>
            <begin position="14"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="74"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="74"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="107"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="136"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="241" mass="27570" checksum="68A55B06277709D9" modified="2018-12-05" version="1">MSVPFSNTHLRVPRGFGALLEGLTREILRDQPDNIPAYAAQYFDKLLKEREESGMDPSEWAAKLEDRFYNNHAFKSTEDEAQSEKEITDTESEREITDTQGESEVTETLKEKEITETKGEGEITETQGEGKITETPGERDKTETPGEREVTETLKEKEITETKGEGKITETXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYIYIIFFIYLFFIPHHNKHTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBW3</accession>
    <name>A0A3B5MBW3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor Fz/Smo family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBW3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017194"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017414.1">
        <property type="protein sequence ID" value="ENSXCOP00000017194.1"/>
        <property type="gene ID" value="ENSXCOG00000012964.1"/>
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        <property type="component" value="Unplaced"/>
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        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
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            <end position="588"/>
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            <dbReference type="Ensembl" id="ENSXCOP00000017194.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="588" mass="65511" checksum="E9D4E1F70152D9ED" modified="2018-12-05" version="1">LLKIISMDCCPLQVVLSLCLLVTSSSSFEIGSYDLDRDRPAKCEPIVIPMCQGIGYNLTRMPNFMQHDDQEEAAIKLLEFAPLVKYGCDVHLRFFLCSLYAPMCTDKVSTSIPACRPMCEQAREKCAPIMKQFSYTWPDSLNCSKLPTRNDPNALCMEAPENETRTEGKKGEGMLPVPHRPWQPGASGARSPGAVGSCENPDKFQFVDKSQSCAPRCSPAVDVYWSRQDKDFAFIWVTVWSILCFVSTAFTVLTFLLEPHRFQYPERPIIFLSMCYNVYSVAFIIRSVAGPENIACDRENGELYIIQEGLESTGCTIVFLILYYFGMASSIWWVILTLTWFLAAGKKWGHEAIEAHSNYFHMAAWGIPALKTIIILTMRKVAGDELTGLCYVGSMDSGALTGFVLIPLSCYLIIGTSFILTGFVALFHIRKVMKTEGTNTEKLEKLMVKIGIYSILYTVPATCVIVCYFYERLNMDYWKLRGLQTKCGSFGGPAGDCSLQTSVPTVAVFMLKIFMSLVVGITSGVWVWSSKTLQTWQGLCSRKLADRTSGRKPCGGVNCGSTHCHYKSPAVVLHMAKTDPHSDSPTHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCR8</accession>
    <name>A0A3B5MCR8_9TELE</name>
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        <recommendedName>
            <fullName evidence="3">HOOK N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
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            <taxon>Xiphophorus</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <property type="entry name" value="Calponin-like domain"/>
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        <property type="entry name" value="CH_dom_sf"/>
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        <property type="entry name" value="HOOK PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947:SF31">
        <property type="entry name" value="PROTEIN DAPLE"/>
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    </dbReference>
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        <property type="entry name" value="Hook domain"/>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="12095" checksum="7F5F45A991E9FB41" modified="2018-12-05" version="1">MDVTLSELLASFMESPLVLWVRMLGPLGSEERVAMFMELVDGVFLHKVMTYIDPNPTNQRLNKNVNNDVSLRLYNLTVLTRHIRTYYQVQNRTHCSRTGPIGPVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCV8</accession>
    <name>A0A3B5MCV8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Leucine zipper putative tumor suppressor 2 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein LAPSER1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LZTS2</name>
        <name evidence="9" type="synonym">LAPSER1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Negative regulator of katanin-mediated microtubule severing and release from the centrosome. Required for central spindle formation and the completion of cytokinesis. Negative regulator of the Wnt signaling pathway. Represses beta-catenin-mediated transcriptional activation by promoting the nuclear exclusion of beta-catenin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
            <location evidence="9">Cytoskeleton</location>
            <location evidence="9">Microtubule organizing center</location>
            <location evidence="9">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the LZTS2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCV8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017534"/>
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        <property type="protein sequence ID" value="ENSXCOP00000017534.1"/>
        <property type="gene ID" value="ENSXCOG00000013220.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:centrosome"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042074">
        <property type="term" value="P:cell migration involved in gastrulation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060026">
        <property type="term" value="P:convergent extension"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009953">
        <property type="term" value="P:dorsal/ventral pattern formation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051013">
        <property type="term" value="P:microtubule severing"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051168">
        <property type="term" value="P:nuclear export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030513">
        <property type="term" value="P:positive regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051255">
        <property type="term" value="P:spindle midzone assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03026">
        <property type="entry name" value="LZTS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045329">
        <property type="entry name" value="LZTS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028597">
        <property type="entry name" value="LZTS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19354">
        <property type="entry name" value="ZIPPER PUTATIVE TUMOR SUPPRESSOR 2 HOMOLOG-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19354:SF4">
        <property type="entry name" value="ZIPPER PUTATIVE TUMOR SUPPRESSOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06818">
        <property type="entry name" value="Fez1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="6 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="194"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="291"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="205"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="252"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="670" mass="74555" checksum="4CC7E3D66285A65A" modified="2018-12-05" version="1">MALVQALPVSDHPNPGLDLHQCRPLSPHSPSDPCSGPCGACSSDTAMGSVSSLISGRTYQERHCRAGSEFTNKPRRSTPATSCFRLQDKTLRSGSSLEQLGGGMAAGAAGGGTNANFGYDNGTVSGNISGPPPKLVPVSGQLEKNMEKVLIRPTAFKPVVPKNRHSVQYLSPRPGGSSLSESQASLNLLLPLAAPNSTSSTNSNVGSRNARSIQSCSMSDSGRNSLSSLPTHSSTGYSLAPSEGSRSGGHGHSNSDSGRSSFSKSTGSGSLSGRGQPLSDSGSCGHSPPPVEGYEVVVKELEEKLRERDQELQQLRENLDENEAAICQVYEEKQRRCEREMEELRQSCAVKMKQTSQKAQRAQQVLQLQVFQLQQEKKKLQEDFSSLLQDRETLERKCATIQREQTQLGPRLEETKWEVCQKSGEISLLKQQLKEIQSELSQKAGDIVVLKAQLREARSELQSSQGRTQEAQTALRTRSLELEVCENELQRRKSEAELLREKVSRLEDESGRIRDTLSNHSGNAPKKGLGMSLSFQQGRVVMGRGGPSPYMYRDGEDSYLVWGGESDEAKAQRQNVDRLKAELMYERRTGEEQRSRFEEERRVWHEEKEKVIRYQKQLQQNYIQMYRRNRDLERVMRELSLELENRDMEDYEVRSGSNDIHFEEITATEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCW0</accession>
    <name>A0A3B5MCW0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCW0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021452"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021714.1">
        <property type="protein sequence ID" value="ENSXCOP00000021452.1"/>
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    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CBX7"/>
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        <property type="entry name" value="CHROMOBOX PROTEIN HOMOLOG 7"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR47277:SF1">
        <property type="entry name" value="CHROMOBOX PROTEIN HOMOLOG 7"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF54160">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="2">
        <location>
            <begin position="11"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="55" mass="6503" checksum="6024153926C301B8" modified="2018-12-05" version="1">MELSSIGEQVFAVESITKKRIRKTVVKYLRICRYSTWEPEDNILDPLLVLAYEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEP2</accession>
    <name>A0A3B5MEP2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fibroblast growth factor</fullName>
            <shortName evidence="3">FGF</shortName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEP2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019691"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019936.1">
        <property type="protein sequence ID" value="ENSXCOP00000019691.1"/>
        <property type="gene ID" value="ENSXCOG00000014693.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158380"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:anatomical structure homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031101">
        <property type="term" value="P:fin regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046850">
        <property type="term" value="P:regulation of bone remodeling"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="Fibroblast growth factor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fibroblast_GF_fam"/>
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    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00263">
        <property type="entry name" value="HBGFFGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0339">Growth factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="54"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="251" mass="28341" checksum="23F596254EE6B353" modified="2018-12-05" version="1">MGAVAEVGSFANGFLDSFGAASASVGSHFVLTPAGAVDYNSATGLMLGGQALLQQHHHHHQQQHQQHQQQQHHHHHHHQQQENLVSAERLSRSLADLSHLKGILRRRQLYCRTGFHLEIHPDGTVEGTRKDHSRFGILEFISLAVGLVSIRGVDSGLYLAMNSKGELYGSEKLSAECVFREQFEENWYNTYSSNIYRHGEKGAFYFVGLNKDGTSRDGTRSRRHQRFTHFLPRPVDPERVPDLYRDILGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFR8</accession>
    <name>A0A3B5MFR8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">LIM domain binding 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the LDB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MFR8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019966"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020213.1">
        <property type="protein sequence ID" value="ENSXCOP00000019966.1"/>
        <property type="gene ID" value="ENSXCOG00000014993.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005639"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030274">
        <property type="term" value="F:LIM domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000063">
        <property type="entry name" value="LIM domain-binding protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041363">
        <property type="entry name" value="LID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029005">
        <property type="entry name" value="LIM-bd/SEUSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10378">
        <property type="entry name" value="LIM DOMAIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17916">
        <property type="entry name" value="LID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01803">
        <property type="entry name" value="LIM_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51957">
        <property type="entry name" value="LID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LIM interaction" evidence="6">
        <location>
            <begin position="293"/>
            <end position="332"/>
        </location>
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        <location>
            <begin position="245"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="325"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="336"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006928"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="368" mass="42192" checksum="C6A9F7E56C7E5E8B" modified="2018-12-05" version="1">MSNPTHDPFYSSPFGPFYRRHSPYMVQPEYRIYEMNKRLQTRTEESDSLWWDAFATEFFEEDATLTLSFCLEDGPKRYTIGRTLIPRYFSTVFEGGVTELHYILKHSKESFHNSCITLDCDQCTMVTQHGKPMFTKVCTEGRLILEFAFDDLMRIKTWHFTIRQYRELIPRSILAMHAQDPQVLEQLSKNITRMGLTNFTLNYLRLCVILEPMQELMSRHKTYNLSPRDCLKTCLFQKWQRMVAPPAEPARQTTTKRRKRKNSASSANSSVGTAASKKRSPATNFSLSSQVPDVMVVGEPTLMGGEFGDEDERLITRLENTQYDATNGLDDEDDFNSSPALGNNAPWSSKPPAAQDSKPESTAPQPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGC3</accession>
    <name>A0A3B5MGC3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGC3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020161"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020408.1">
        <property type="protein sequence ID" value="ENSXCOP00000020161.1"/>
        <property type="gene ID" value="ENSXCOG00000015111.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000155601"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
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        <property type="term" value="P:calcium ion import across plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
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    </dbReference>
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        <property type="entry name" value="Voltage-dependent N-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
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    </dbReference>
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        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050599">
        <property type="entry name" value="VDCC_alpha-1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45628:SF5">
        <property type="entry name" value="VOLTAGE-DEPENDENT R-TYPE CALCIUM CHANNEL SUBUNIT ALPHA-1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
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    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
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    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
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    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="134"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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            <dbReference type="Pfam" id="PF00520"/>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020161.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    </evidence>
    <sequence length="282" mass="31506" checksum="62C4A52D5240B8D5" modified="2018-12-05" version="1">MARFGDESAPATVDSGDGDLERGGDGQDAASGGLTASMKQAKAQRARTMALYNPVPHRQNCLTVDRSLFIFAEDNIIRKYARRIIEWPYPYMKSMICYMILATITANCVVLALEQHLPGEDKTPMAKRLEKTEPYFIGIFCFEAGIKLVALGFVFHKGSYLRNGWNVMDFIVVLSGILATAGAHMNIPVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYSGKLHHTLWEEGEKRDRIQDRSRNQSSEKVNLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHJ0</accession>
    <name>A0A3B5MHJ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thyroglobulin type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Acanthomorphata</taxon>
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            <taxon>Atherinomorphae</taxon>
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            <taxon>Poeciliinae</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHJ0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023222"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023500.1">
        <property type="protein sequence ID" value="ENSXCOP00000023222.1"/>
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    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="NIDOGEN-2"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12352">
        <property type="entry name" value="SECRETED MODULAR CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00086">
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57610">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="domain" description="Thyroglobulin type-1" evidence="7">
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            <begin position="22"/>
            <end position="89"/>
        </location>
    </feature>
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    </feature>
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            <begin position="141"/>
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    </feature>
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            <dbReference type="ARBA" id="ARBA00022525"/>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="191" mass="20784" checksum="A9C620F178ECA3A3" modified="2018-12-05" version="1">EPGLDLVHLVSTAEQVRLMRPKTPCEHHKERVQASGSREYPVAGAYVPQCDTDGQYIPLQNFTTSLCWCFNGQERAGTRTPPGAQPRDCVKPGKRVGFIAPKTPCEEQRDRAQASIQERYPVAGVFVPRCDAQGQFVSQQCHGSTGHCWCVDSSGQERAGSRTAPGASPVDCRKPGKKAASSRGRMTSAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIH2</accession>
    <name>A0A3B5MIH2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GED domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIH2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019359"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019597.1">
        <property type="protein sequence ID" value="ENSXCOP00000019359.1"/>
        <property type="gene ID" value="ENSXCOG00000014552.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155214"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:microtubule"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:GTPase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:microtubule binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016185">
        <property type="term" value="P:synaptic vesicle budding from presynaptic endocytic zone membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Dynamin, middle domain"/>
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    </dbReference>
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        <property type="entry name" value="Dynamin"/>
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        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF23">
        <property type="entry name" value="DYNAMIN-2"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF02212">
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    </dbReference>
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        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GED" evidence="2">
        <location>
            <begin position="9"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="200" mass="21526" checksum="FBB71E8800D381D0" modified="2018-12-05" version="1">MDPQLERQVETIRNLVDSYISIVNKSIRDLMPKTIMHLMINNTKDFIHSELLAYLYSAGDQGSLMEESAEKAQRRDEMLRMYHALKEALVIIGDISTTTISTPVPPPVDDTWIQHLALCFSPPAASRPLASTAAPPNRPPAARGPTAGPPAPLNPSPAFGAPPVPSRPGGPSPYDANSAVPLIPSRPPRVPPALPPGIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIU2</accession>
    <name>A0A3B5MIU2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Caudal type homeobox 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Caudal homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIU2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021036"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021294.1">
        <property type="protein sequence ID" value="ENSXCOP00000021036.1"/>
        <property type="gene ID" value="ENSXCOG00000015739.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009948">
        <property type="term" value="P:anterior/posterior axis specification"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0048617">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060914">
        <property type="term" value="P:heart formation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:hindbrain-spinal cord boundary formation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003311">
        <property type="term" value="P:pancreatic D cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036342">
        <property type="term" value="P:post-anal tail morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048793">
        <property type="term" value="P:pronephros development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048384">
        <property type="term" value="P:retinoic acid receptor signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001756">
        <property type="term" value="P:somitogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Caudal type homeobox 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006820">
        <property type="entry name" value="Caudal_activation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047152">
        <property type="entry name" value="Caudal_homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24332">
        <property type="entry name" value="HOMEOBOX PROTEIN CDX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24332:SF15">
        <property type="entry name" value="HOMEOBOX PROTEIN CDX-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04731">
        <property type="entry name" value="Caudal_act"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="146"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="148"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="209"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="129"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="225"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="268" mass="30061" checksum="E0451323FFD82791" modified="2018-12-05" version="1">MWENFKSGMYHQGPVRRSSINLPPQNFVSSPQYPDFTGYHHVPNMDTHAQSGASWGPSYGAPREDWGAPYSLGPPNNLPPPMNNSSPGQVPYCSSEYSHMHPPGAAVLQPPPDNVTVAQLSPERERRNSYQWMSKTAQASSTGKTRTKEKYRVVYTDHQRLELEKEFHFNRYITIRRKSELAVNLGLSERQVKIWFQNRRAKERKLIKKKLGQSDGSSGSVHSDPGSVSPLPVPGSLSPTEIHGSLYPPQGMNTLPSIRNIQQVTVTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJ76</accession>
    <name>A0A3B5MJ76_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zgc:162297</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BtpA family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027869658.1">
        <property type="nucleotide sequence ID" value="XM_028013857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJ76"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023812"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024096.1">
        <property type="protein sequence ID" value="ENSXCOP00000023812.1"/>
        <property type="gene ID" value="ENSXCOG00000017787.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114142530"/>
    <dbReference type="KEGG" id="xco:114142530"/>
    <dbReference type="GeneTree" id="ENSGT00390000006020"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005137">
        <property type="entry name" value="BtpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00259">
        <property type="entry name" value="thylakoid_BtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21381:SF3">
        <property type="entry name" value="SGC REGION PROTEIN SGCQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21381">
        <property type="entry name" value="ZGC:162297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03437">
        <property type="entry name" value="BtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005956">
        <property type="entry name" value="BtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="271" mass="29020" checksum="75CA8D40DBF4F60F" modified="2018-12-05" version="1">MKFQSLFGSLKSVVIGMIHVKALPGTPLGCLNMLQIVKEACGEAEVYLEAGIDGLIIENMHDVPYSFNVGPEVCACMTAVCSAVRHVCPTLPLGVQILSSANQQALAVALASGLDFIRAEGFVFSHVADEGLLNACAGDLLRYRKQIGADGVQIFTDIKKKHSSHALTADVTIKETARAAEFFLSDGVIITGAATGDQADPCELTEVSQSVKIPVLIGSGVTYNNVENFLDARGMIIGSHFKLGGHWANAIDPDRVKRFMAKMHHLRKPQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJF8</accession>
    <name>A0A3B5MJF8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027860845.1">
        <property type="nucleotide sequence ID" value="XM_028005044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJF8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019924.1">
        <property type="protein sequence ID" value="ENSXCOP00000019679.1"/>
        <property type="gene ID" value="ENSXCOG00000014781.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114136774"/>
    <dbReference type="KEGG" id="xco:114136774"/>
    <dbReference type="CTD" id="566349"/>
    <dbReference type="GeneTree" id="ENSGT00390000009209"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040288">
        <property type="entry name" value="PXDC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31433">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31433:SF0">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="1">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="233" mass="27219" checksum="80D4968AF6FD975D" modified="2018-12-05" version="1">MASAVFEGTSLVNMFVRDCWVNGIRRLIISQRGEEEEFFEIRTEWSDRNILYLHRSYSDLGRLFKRLLKSFPEDRKDLSKSPLIEGLVKIKDAHDIEEKLNEVERLLKNAINMPCKFSRSEVMLTFFERSPLDQVLRNDKVHRIQPCFQSPINISEIMRSNGFCLANTETIVFDDSIPEEKERPSSTDSGEHTYEDGTEFLPMEADVCEEETEAYVTNLSYYHLVPFETDILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKH5</accession>
    <name>A0A3B5MKH5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL46</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L46, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL46 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027870452.1">
        <property type="nucleotide sequence ID" value="XM_028014651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKH5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024252"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024543.1">
        <property type="protein sequence ID" value="ENSXCOP00000024252.1"/>
        <property type="gene ID" value="ENSXCOG00000018106.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114142984"/>
    <dbReference type="KEGG" id="xco:114142984"/>
    <dbReference type="CTD" id="26589"/>
    <dbReference type="GeneTree" id="ENSGT00390000015400"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04661">
        <property type="entry name" value="NUDIX_MRP_L46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000018">
        <property type="entry name" value="39S ribosomal protein L46, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040008">
        <property type="entry name" value="Ribosomal_mL46"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021757">
        <property type="entry name" value="Ribosomal_mL46_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033650">
        <property type="entry name" value="Ribosomal_mL46_NUDIX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13124">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L46, MITOCHONDRIAL PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13124:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11788">
        <property type="entry name" value="MRP-L46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Large ribosomal subunit protein mL46 N-terminal" evidence="9">
        <location>
            <begin position="56"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="292" mass="32787" checksum="38029FBD5F33D7F0" modified="2018-12-05" version="1">MAAPCRKMASRSFPQLLCYFSRIAVGSGACRRFSVTPIDRATPRASTAADRATSPWSLMAAVCLQRLPVITADCSPIEQRFKQMLQQMELEKSLVSDHELRLMEDAERMSRRQADDYDSDEEDRGDQEIMMTQDLEDTWEQRLKNFQPAPRVRADVDKDLSSAQRCLADSLVLLAEQQVGAERLWLLPQAAWQEGETLRQTAERALDSVTAAGVKVTFLGNAPCGVYKYKLPKAARTEGSVGRKVFFFKAILADGAPGSAPTAPLLWVKKSELQKYLKPSYMMKVDRFILNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLF4</accession>
    <name>A0A3B5MLF4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Proteasome assembly chaperone 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PSMG1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027887885.1">
        <property type="nucleotide sequence ID" value="XM_028032084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLF4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025229"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025534.1">
        <property type="protein sequence ID" value="ENSXCOP00000025229.1"/>
        <property type="gene ID" value="ENSXCOG00000018843.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114153498"/>
    <dbReference type="KEGG" id="xco:114153498"/>
    <dbReference type="CTD" id="8624"/>
    <dbReference type="GeneTree" id="ENSGT00500000044950"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080129">
        <property type="term" value="P:proteasome core complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016565">
        <property type="entry name" value="Proteasome_assmbl_chp_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15069">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15069:SF1">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16094">
        <property type="entry name" value="PAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="9"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="279" mass="30910" checksum="393A8055FDBCA7D2" modified="2018-12-05" version="1">MATFFGEVLSVYSRAVEEDDEELEENEEDEQIRRELEEKRTVHLHWNPEISELLRAGNKVQCSSFILAVGHNAASFLSAYILASANWDVVGRAVAWNEMSRSGTGPSGEGSACVFYRHKDEPAALICHVTCQMAEDQLFQWTEKVFGYLQTRGLTVTVLSDSSVADYKTADYLCSSSAPFLRSLCSSAFSDQPVCQSLEQPNIITGLPAAVLNHCQVHSIAAAVYQCYSDVTGPDSITMDKYKPVLTKLGRSIQVYSSQSTDVLCKFVRSSEIQSNLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLZ9</accession>
    <name>A0A3B5MLZ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Soluble scavenger receptor cysteine-rich domain-containing protein SSC5D</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to extracellular matrix proteins. Binds to pathogen-associated molecular patterns (PAMPs) present on the cell walls of Gram-positive and Gram-negative bacteria and fungi, behaving as a pattern recognition receptor (PRR). Induces bacterial and fungal aggregation and subsequent inhibition of PAMP-induced cytokine release. Does not possess intrinsic bactericidal activity. May play a role in the innate defense and homeostasis of certain epithelial surfaces.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with LGALS1 and laminin.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLZ9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022171"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022440.1">
        <property type="protein sequence ID" value="ENSXCOP00000022171.1"/>
        <property type="gene ID" value="ENSXCOG00000016564.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183145"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000001">
        <property type="entry name" value="Lysyl oxidase 4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000006">
        <property type="entry name" value="neurotrypsin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000007">
        <property type="entry name" value="Soluble scavenger receptor cysteine-rich domain-containing protein SSC5D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF27">
        <property type="entry name" value="SCAVENGER RECEPTOR CYSTEINE-RICH TYPE 1 PROTEIN M130-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="26"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="129"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="10">
        <location>
            <begin position="236"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="50"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="63"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="153"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00064153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="308" mass="32259" checksum="D57455C9A4586785" modified="2018-12-05" version="1">MDVFLQPAVFISPVSPLCLSVVDAQTRLVGPSRCSGRVEVYHDEVWGTVCDDSWDLSDAAVVCRQLGCGPPKSAPTVAYFGEGTDPIWLDDVACAGTESSLADCLHRGFGSHNCGHVEDAGVICEEAQFRLAGPTRCSGRVEVYHGDAWGTVCDDGWDLSDAAVVCRQLGCGAALGAPTSAYFGQGTGEIWLSNLSCSGREVSLSGCSNPGFGIQDCDHSEDSAKQIQTHFSGAQIRLTGPTRCSGRVEVLFNDTWGTVCDDGWDLSDAAVVCRQLGCGPPQAALSAASFGEGTGPVWLADMSCSGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MN25</accession>
    <name>A0A3B5MN25_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MN25"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022556"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022831.1">
        <property type="protein sequence ID" value="ENSXCOP00000022556.1"/>
        <property type="gene ID" value="ENSXCOG00000016864.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074870"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="164" mass="18561" checksum="9A8F6E65234DA3D8" modified="2018-12-05" version="1">MAERGYSFSLTTFRVLVRRARKLAQQYYLVYQEPIPTGQLVQRVASVMQEYTQSGGVRPFGVSLLIAGWDEDHPYLFQSDPSGAYFAWKATAMGKNYVNGKTFLEKRYNNDLELEDAIHTAILTLKESFEGQMTEENIEVGICNEAGFKRLTPAEVKDYLAAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MN94</accession>
    <name>A0A3B5MN94_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Oligosaccharyltransferase complex subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6 8">Specific component of the STT3A-containing form of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Specific component of the STT3A-containing form of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the OSTC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MN94"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020934"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021191.1">
        <property type="protein sequence ID" value="ENSXCOP00000020934.1"/>
        <property type="gene ID" value="ENSXCOG00000015672.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001376"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021149">
        <property type="entry name" value="OligosaccharylTrfase_OST3/OST6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042416">
        <property type="entry name" value="OSTC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13160">
        <property type="entry name" value="OLIGOSACCHARYLTRANSFERASE COMPLEX SUBUNIT OSTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13160:SF4">
        <property type="entry name" value="OLIGOSACCHARYLTRANSFERASE COMPLEX SUBUNIT OSTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04756">
        <property type="entry name" value="OST3_OST6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="184" mass="20825" checksum="24261C8A30F3E97B" modified="2018-12-05" version="1">MYFRDTSTKSVRRRTRAWAERSAAGEKDATFFCSIMETLYSFPFAVLECPNIKLKKPSWLHMPSAMTVYAVVIVSYFLITGGIIYDVIVEPPSVGSMTDEHGHQRPVAFLAYRVNGQYIMEGLASSFLFTMGGLGFIILDRSNAPNIPKLNRFLLLFIGFVSVLLSFFMARVFMRMKLPGYLMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNU0</accession>
    <name>A0A3B5MNU0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Myosin, heavy chain 14, non-muscle</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000026079"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026393.1">
        <property type="protein sequence ID" value="ENSXCOP00000026079.1"/>
        <property type="gene ID" value="ENSXCOG00000019480.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158808"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.360:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1590:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000002">
        <property type="entry name" value="Myosin heavy chain 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000009">
        <property type="entry name" value="myosin-11 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000101">
        <property type="entry name" value="Slow myosin heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
        <property type="entry name" value="Myosin S1 fragment, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myosin, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF857">
        <property type="entry name" value="MYOSIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
        <property type="entry name" value="Myosin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90257">
        <property type="entry name" value="Myosin rod fragments"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51844">
        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 9" id="KW-0518">Myosin</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin N-terminal SH3-like" evidence="12">
        <location>
            <begin position="41"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="11">
        <location>
            <begin position="95"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="9">
        <location>
            <begin position="670"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="937"/>
            <end position="968"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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            <end position="1182"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1248"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
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            <end position="1400"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1703"/>
            <end position="1728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1889"/>
            <end position="1921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1133"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
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            <end position="1267"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
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            <begin position="1356"/>
            <end position="1370"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1371"/>
            <end position="1381"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1703"/>
            <end position="1715"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="188"/>
            <end position="195"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1934" mass="223923" checksum="005A03AB1B9480E9" modified="2018-12-05" version="1">MSRPTGGVANDVTRFLSSGVGTSSPTASSSMFSAASQADWAAKRLVWVPSEKHGFESASIREERGDEVEVELTDSQRKLTLSREEVQRMNPPRFSKVEDMADLTCLNEASVLHNLRERYYSGLIYTYSGLFCVVVNPYKNLPIYTESIVEMYRGKKRHEMPPHIYAISEAAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSVRVSQGELERQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVAGYIVGANIETYLLEKSRATRQAKDERTFHIFYQLLCGASEETRADLLLGSADDYRFLSGGSIPVPGQSDAENFTQTMDSVAIMGFTPEESVSMLKVISAVLQFGNISFTKEKNHDQASMPDNTAAQKLCHLLGINIMEFTRAILTPKIKVGREYVQKAQTKEQADFAIEALAKATYERLFRWLVHRINRALDRRQRQGASFIGILDIAGFEIFQLNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIERPAHPPGVLALLDEECWFPRATDRTFVEKLCSEQGNHSKFYKSKQPRGEADFSIIHYAGKVDYKANDWLIKNMDPLNDNVASLLHQSSDHFVSELWKEVDRIVGLDQVSTGESSGSVPFGAAGLKTKKGMFRTVGQLYKESLTKLMATLRNTNPNFLRCIIPNHEKRAGKLAPHLVLDQLRCNGVLEGIRICRQGFPNRIPFQEFRQRYEILTPNAIPRTFMDGKQASELMINALELDPNLYRVGQSKVFFRAGVLAHLEEERDLKITDTIIRFQSASRGFLARKAFMKKQQQLSAVRVMQRNCAAYLKLRNWQWWRLFTKVKPLLQVTRQDEEIQARESELHKAKENLTRVEQEYTELDRKHTQLIEEKAVLADQLQAEAELFAEAEEMRARLASRKQELEEVLSEMESRLVEEEERGAQLANEKKRMQQNVQDLEEQLEEEESARQRLLLEKVTLETKVKSLEADLLTAVEQRDRLSKEKKLLEEHLSEVTDQLTEEEEKSKSLNKLKNKQEAVIADLEERLKREEQGRLEQEKWKRRMESDSLEAQEQLSDLGMLTAELRGTLSHKEKEITTLQGRLEEEGARRAEAQRSLREALSQVSELKEEVENERGMRERAEKQRRDLGEELEALRTELEDTLDTTATQQELRSRREAELSELQRCVEEETRRHEAQLSELRIKHSAAIDNLQEQLDNSKRARQSLEKAKAVLEEERQNLTEEIKNLQTSRSESERGRKRADGQLQELNARLAQADREREDREERMHKLQIEGLSGNLSSSDSKSLRLAKEVSSLESQLHDVKELLQDETRQKMALASRVRALEEEKNGLMERLEEEEERAKELSRQIQTHSQQLRKQSEEVSTAVEAGEETRRKLQRELDSAFQKERQKEEEKERVERQRERLREELEDMTIALQRERQNCTALEKRQKKFDQCLAEEKGVSARLAEERDRAEADSREKETKCLFLSRSLQEVQDQRDELERANKQLRLEMEQLVNQQDDVGKNVHELERSKRSLETEVQNLRTQTQELEEELSEAENLRLRLEVNLQALKAQFEREISNNEEKGEEKRRALSKQVKELEIHLEEERTQRSQAVAAKKQLEAELQEAEAQLEAASRGKEDSVKQLRRLQGQMKEILRELDETKLSKDEVIAQSKDGEKKIQTLEAEVLHLTEELAVSDRQRRQAQQERDEMSEEMVSSSSGKNVLFDEKRRLEVRVTQLEEDLEEEQTNAELLIERQRKTALQVETLTVQLQGERTLAQKAESSREQLEKQNKELRARLEELEGAVRGKHKLSVAALEAKIETMEEQLEQERQAIANKLTRKTEKKLKEAMMQADDERRHADQYREQLDKSMVRLKQLKRQLEEVEEENSRSNAQKRKLQRELEELTDSSQTMTREISSLRTQLSFCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPE6</accession>
    <name>A0A3B5MPE6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MPE6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025406"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025713.1">
        <property type="protein sequence ID" value="ENSXCOP00000025406.1"/>
        <property type="gene ID" value="ENSXCOG00000018976.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014743"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000036">
        <property type="term" value="P:regulation of stem cell population maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012270">
        <property type="entry name" value="CCR4-NOT_su3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007207">
        <property type="entry name" value="Not_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04065">
        <property type="entry name" value="Not3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005290">
        <property type="entry name" value="NOT_su_3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-Not complex component Not N-terminal" evidence="11">
        <location>
            <begin position="4"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="12">
        <location>
            <begin position="739"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="234"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="339"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="449"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="509"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="257"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="353"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="390"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="456"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="513"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="544"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="565"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="584"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="632"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="843" mass="89283" checksum="63A7C531ED66CA9F" modified="2018-12-05" version="1">MADKRKLQGEIDRCLKKVAEGVEQFEDIWQKLHNAANTNQKEKYEADLKKEIKKLQRLRDQIKTWVASNEIKDKRQLIENRKLIETQMERFKIVERETKTKAYSKEGLGLIQKVDPAQKEKEEVGTWLTNTIDTLNMQVDQFESEVESLSVQTRKKKGDKEKQDRIEELKKFIEKHRYHIRMLETILRMLDNDSLQVESIRKIKDDVEYYRESSQDPDFEENDYIYDDLDLDEFPQPLLATSPPGPSHLEDEIFQNSSSTPTSTTSSSPIPPSPATCTSPVKNGNPSSSLSSSSSSSSSSSSASGLTSSSLVSVAGRGIGGSGSSHLSAVGGPFSNTLASSYSNATQQPPHPSVQQQQAKNSPSSSAPINSSTSTNSHNASSASSPPSTQGLLTSNSLPQAPSGPTATSNSLGLSLGPSLGKSSISTSPGLSGMPASLSNMVGLLSSSTPTPYAQAAASGTASSGLPGSLGGVSSTTTNSGVGMLGTLGTASIVGSVGSGILGLGLGSGQSGVQGSSLGPQSPIGSLAPGSGIGVIGSNSGSSGSAGSGVGGGSSSLSGRPPSRPKQNGSTSHSAVVADSTTDSALNSATQSQSSQSSSLTSTANQLKDTGPSLLGSMGLSSTSPLPVSYSEAKTTSGGSLQNGPLSYSQPPESIKEPLSRSMAERAAPSSGIEGDVSSLHLTSDIFPSSTTTPSGPPSAPQSSLSEVSILPSLGVCPLGPVPLSKDQLYQQAMQEAAWTHMPHPSDSERIRQYLMRNPCPTPPFHHQMPPPHSDTVEFYQRLSTETLFFIFYYLEGTKAQYLAAKALKKQSWRFHTKYMMWFQRHEEPKTITDEYEQVSLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQ92</accession>
    <name>A0A3B5MQ92_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">2Fe-2S ferredoxin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adrenodoxin/putidaredoxin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQ92"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023346"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023626.1">
        <property type="protein sequence ID" value="ENSXCOP00000023346.1"/>
        <property type="gene ID" value="ENSXCOG00000017435.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156916"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000013">
        <property type="entry name" value="Adrenodoxin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018298">
        <property type="entry name" value="Adrenodoxin_Fe-S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426:SF75">
        <property type="entry name" value="ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="FERREDOXIN/ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00355">
        <property type="entry name" value="ADRENODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00814">
        <property type="entry name" value="ADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="11">
        <location>
            <begin position="70"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="185" mass="20639" checksum="1149C45E2CFAE363" modified="2018-12-05" version="1">MFAEILVKLWGCWRKRHNVIYKQFFTLKEYVQLRRYRQRSASSQVNLPFSVSDRRAAGSGGSALPCVFKVTVHFINRDGEKITVKASPGDSLLDVVINEDLNFDGFGACEGTLACSTCHLIFDEEVYKKLGPVTDEEMDMLDLAYGLTETSRLGCQICLTKSLEGMVARVPESVADIRQTKDGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQP4</accession>
    <name>A0A3B5MQP4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cell adhesion molecule 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nectin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQP4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026098"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026412.1">
        <property type="protein sequence ID" value="ENSXCOP00000026098.1"/>
        <property type="gene ID" value="ENSXCOG00000019491.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159779"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000013">
        <property type="entry name" value="cell adhesion molecule 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003585">
        <property type="entry name" value="Neurexin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45889:SF5">
        <property type="entry name" value="CELL ADHESION MOLECULE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45889">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00294">
        <property type="entry name" value="4.1m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="386"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="61"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="186"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="286"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="452" mass="49329" checksum="738A5804ADFD47D1" modified="2018-12-05" version="1">MFFCLLTLESPQAPQFPSVVSLLSPPFFFSHSPGFPPRFATQPPLHTLIHPSFPLCRRSLPKFTVAALQLSFVTFLFYLDEDPLQPMTSDETVSVGGMVTLTCRVAETDNSSLQWSNTAQQTLYFGEKRALRDNRIQLHRSTPTELSINISDVQLSDEGEYTCSIFTMPVRTARATVTVLGVPGTPQISGFENPVPEGEKVTLTCTSTGSKPPAKLRWYKGDIEVQGRPDVVESVPDDPTYNVSSELTLEVTANDDDTKISCVVDHDSVKHGEKKSEQSLRVLYAPDVAITPEERMAIEGQRFSLQCSGKGNPEPTIYSWEKAEGELPPLAKADGAFLSFSVLNKSDNGMYICHADNGIGKGRGSYTLQVQDTTAMSTSSGVDHAVIGGVVAVIVFILLCLLIVLGRYLIRHKGTYLTHEAKGSDDAPDADTAIINAEGGHSGAEEKKEYFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRT3</accession>
    <name>A0A3B5MRT3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRT3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022204"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022473.1">
        <property type="protein sequence ID" value="ENSXCOP00000022204.1"/>
        <property type="gene ID" value="ENSXCOG00000016587.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01070000254339"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF87">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Immunoglobulin V-set" evidence="4">
        <location>
            <begin position="27"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="216" mass="24216" checksum="945BCA807013BD38" modified="2018-12-05" version="1">MFESRNVTPDRCCKPTAVLDRRSGPQQKRVSAEPGSSVTVACWFALSGGTKYFCRGDCGGNALVQTAGESAHSGRYSIQHERKLSAEGVLLVTITELTQSDSGRYHFDITVTPVHFNPPTIVLRVWAAGTRSRKRSYFILEMIPQRLRSNFLFILGLWKMRLSLGFLKVFSDVYPWKPAASVSAHCSRNQQHQSHRCGSCLRSHFFKIFPCKRSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSM1</accession>
    <name>A0A3B5MSM1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Kelch domain-containing protein 10</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the KLHDC10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSM1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027399"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027732.1">
        <property type="protein sequence ID" value="ENSXCOP00000027399.1"/>
        <property type="gene ID" value="ENSXCOG00000020456.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155977"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032874">
        <property type="term" value="P:positive regulation of stress-activated MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000009">
        <property type="entry name" value="Kelch domain-containing protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000010">
        <property type="entry name" value="kelch domain-containing protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052125">
        <property type="entry name" value="KLHDC10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46428">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46428:SF1">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="383" mass="43682" checksum="EDFF7322E2719CF3" modified="2018-12-05" version="1">MPSLEPNGTSSHLNEFEKLSWRPSVRHCIPSARSGHRCVADSSNLYVFGGYNPDFEEAGGSENEDYPLFRELWRFHFATATWQQIRTEGYMPTELASMSAVLHGNNLLVFGGTGIPFGENNSNDVHVCNVLYKRWNLLNCKGKKPNKIYGQAMVIINGYLYVFGGTTGYLYSTDLHRLDLSTREWTHLKPKNTPLDLPDERYRHELAQDRQRIYILGGGTSWMSYPLDKIHAYNLETNYWEEIVTKPHEKTGYPAARRCHSCVQVKDDVFICGGYNGELILSDLWKINLQTFQWMKLPAVMPEPAYFHSAAVTPAGCMYIHGGVVNMSGNQRTASLYKVWLVVPSLLEMTWEKLLKLFPHLTQLSTLQLLSLGLTHSLIQRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUD6</accession>
    <name>A0A3B5MUD6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MUD6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000028029"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028370.1">
        <property type="protein sequence ID" value="ENSXCOP00000028029.1"/>
        <property type="gene ID" value="ENSXCOG00000020910.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165144"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05502">
        <property type="entry name" value="Bromo_tif1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45915">
        <property type="entry name" value="TRANSCRIPTION INTERMEDIARY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45915:SF7">
        <property type="entry name" value="TRIPARTITE MOTIF-CONTAINING PROTEIN 66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Bromo" evidence="9">
        <location>
            <begin position="216"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="24"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000028029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="342" mass="38812" checksum="4DB2F759E14F68F0" modified="2018-12-05" version="1">FSVGPPQSDECQVETVSACWTEPYCPAGSPYGEPEQDSGFDCESNPDQPYILLDSGSDDGRSETETFYLDPDAKQAVMMELDPEANRDLCVQLEDDPDVKCEADVLETELSEDGGTDLCCLTKPELESGAGTEQTEESEDFCAVCLNGGELLCCDRCPKVYHLDCHVPSLMSFPNYEFSNLLQLLLQILVNSVPLSSCAFFFFVLQRCEKLSLMLYCHPLSVPFHDPVSPLARNYYQIIKRPIDLSVIRRKLDKCNTLHYFTAEQFVDDILLMFKNCATFNYPDSEVAQAGRTLEVFFLSSLKEVFPERTFPSASQDRMNKARNRWLSRKRRHAFSGRKFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWG5</accession>
    <name>A0A3B5MWG5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Glucose-6-phosphatase</fullName>
            <ecNumber evidence="4 11">3.1.3.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">G6PC2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2 11">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glucose-6-phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.9" evidence="4 11"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWG5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025762"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026072.1">
        <property type="protein sequence ID" value="ENSXCOP00000025762.1"/>
        <property type="gene ID" value="ENSXCOG00000019256.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183150"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004346">
        <property type="term" value="F:glucose-6-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03381">
        <property type="entry name" value="PAP2_glucose_6_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016275">
        <property type="entry name" value="Glucose-6-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12591">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12591:SF1">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000905">
        <property type="entry name" value="Glucose-6-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 11" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="280"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="309"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="15">
        <location>
            <begin position="53"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000905-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000905-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="345" mass="39671" checksum="FB49EE439EC62BEC" modified="2018-12-05" version="1">MDFIHTSGVLIIQHLQNNYREYNSFLSFMSIVGDPRNIFYVYFPVWFHLSHNVGTKMIWVAVVGDWFNLIFKWILYGQRPYWWVHETHLFQNSSKPHLDQFHITCETGPGSPSGHAMGSSCVWYVMVTSALAFTRFHLLKSILWTAFWVIQISVCISRVFIATHFPHQVVLGLVIGMLVAEAFDYITWIYNASLKRYLLVNIFLLSFAICFYLLLKLLDFDPLWSVTKAKMWCANPDWIHLDTTPFAGLCRNLGALFGLGLAVNSEMFIQSCKGKNSCNAAFKLLCMTANLTILQLYDLFKMPNHSEALFYLLSFCKSASVPFGVVAVIPYCVCLLIRDDERKLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWK6</accession>
    <name>A0A3B5MWK6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Epsin 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the epsin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWK6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024104"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024390.1">
        <property type="protein sequence ID" value="ENSXCOP00000024104.1"/>
        <property type="gene ID" value="ENSXCOG00000017991.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157239"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16990">
        <property type="entry name" value="ENTH_Epsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000002">
        <property type="entry name" value="epsin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF50">
        <property type="entry name" value="EPSIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ENTH" evidence="8">
        <location>
            <begin position="11"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="141"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="310"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="141"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="175"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="539" mass="58339" checksum="7962A68E0D56699C" modified="2018-12-05" version="1">MPSTIRRQMKNMVNNYSDAEKKVREATSNDPWGPSSSLMSEVADLTYNVVAFSEIMNMIWKRLNDHGKNWRHVYKALTLLDYLIKTGSERVALQCKENIFAIQTLKDFQYIDKDGKDQGINVREKSKQLVVLLKDEERLKGERSQALKTKERMAQVSTGSTQMGFGRGSSQPNLSTSYSEEYGRSEGSPASYHGSTSPNAGSELEQARPQTSGEEELQLQLALAMSREAAEQEERLRRGDDLRLQMALEESKKEGPGSAKLPKKKKEVRLPWNLTALVTQLLHLLKYALETALSVFFTLLMSDWKECSAPRPSVPPDPWAAPASLPTMKASDPWANSAPATDPWGSGAGNFDLFSTSNGTSKEDFSEFDNLRSSSSVPTADAGITSSLPSQVSLASSGSLDLFDPLPTSTLISTNPTRKTPESFLGPNAALVNLDSLVTKPAQPAPIVNPFLAATGGSAPAPLANPFQMTQPVPPTLNQMRVSPMPSGFGSMAEPMPLASVPAQPVAMVPLAGMAPMGHGIMPPQAGSQPAGTTNPFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZV8</accession>
    <name>A0A3B5MZV8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA-directed RNA polymerase III subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPC7 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MZV8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027817"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028152.1">
        <property type="protein sequence ID" value="ENSXCOP00000027817.1"/>
        <property type="gene ID" value="ENSXCOG00000020741.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169539"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024661">
        <property type="entry name" value="RNA_pol_III_Rpc31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15367">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15367:SF4">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC7-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11705">
        <property type="entry name" value="RNA_pol_3_Rpc31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="120"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="126"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="20581" checksum="1CBA187D3B00E70D" modified="2018-12-05" version="1">MAGRGRGRRTLGLDSVGTSRGDSLPPAVQQPTTVFPVMEQKPLPLAGGEESEYLTALKQDFRVAMRSQPCFIQPAAAHRDVERYSDKYHLSDKMDGLTDWIIGEFNSIRNSSFQFSLTLEKKEQQESSEEEGEEQKEDEAEAEEEYDEEEFEEETDYIMSYFDNGEDFGGDSDDNMDEAVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZY6</accession>
    <name>A0A3B5MZY6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta/gamma crystallin 'Greek key' domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MZY6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028198.1">
        <property type="protein sequence ID" value="ENSXCOP00000027862.1"/>
        <property type="gene ID" value="ENSXCOG00000020780.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163149"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000001">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF62">
        <property type="entry name" value="CRYGM2B PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="39"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="128"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="174" mass="21363" checksum="E70494FB5D31F6D2" modified="2018-12-05" version="1">MVIFYEDRNFQGRSYDCSSDCADMSSYLSRCHSCRVERGCFMVYDRTNYMGNQYFMRRGEYADYMSMMGMSDCIRSCRMIPMYRGSYRMRIYERENYGGQMYEMMDDCDNITDRYRMSNCMSCHVMDGHWLMYEQPHYRGRMMYMRPGEYRNFTNMGLSNMRFMSMRRIMDSYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N126</accession>
    <name>A0A3B5N126_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA polymerase II elongation factor ELL N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5N126"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027120"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027453.1">
        <property type="protein sequence ID" value="ENSXCOP00000027120.1"/>
        <property type="gene ID" value="ENSXCOG00000020247.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164943"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031176">
        <property type="entry name" value="ELL/occludin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019464">
        <property type="entry name" value="ELL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288">
        <property type="entry name" value="OCCLUDIN AND RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23288:SF12">
        <property type="entry name" value="RNA POLYMERASE II ELONGATION FACTOR ELL2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10390">
        <property type="entry name" value="ELL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA polymerase II elongation factor ELL N-terminal" evidence="1">
        <location>
            <begin position="19"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="114" mass="13144" checksum="50EA87F54328CFB3" modified="2018-12-05" version="1">MFLTVSHSYFFCFYLWQYIKIPAPTTESPDGFRVFSFYLSSDSKDKPQSSFDCIHQFISGEGRDHLESQGSIQDKITVCATDDSYQTTRERMSQVLKKSVSSCCIMSCVHNVYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KMN5</accession>
    <name>A0A3B5KMN5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CLIP1 zinc knuckle domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KMN5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001363"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001386.1">
        <property type="protein sequence ID" value="ENSXCOP00000001363.1"/>
        <property type="gene ID" value="ENSXCOG00000001117.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155122"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032108">
        <property type="entry name" value="CLIP1_ZNF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16641">
        <property type="entry name" value="CLIP1_ZNF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CLIP1 zinc knuckle" evidence="7">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="CLIP1 zinc knuckle" evidence="7">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="99" mass="11355" checksum="4A275C3E81F7A0EB" modified="2018-12-05" version="1">LKDKLEKMAATALNGNSPSELDNNYDKQPEKKKPPPRKFCDICDCFDLHETEDCPLQEQMPDSPPHTTYHGSKGEERPYCDTCEVFGHWTESCTDDQTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KNB0</accession>
    <name>A0A3B5KNB0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KNB0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001603"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001628.1">
        <property type="protein sequence ID" value="ENSXCOP00000001603.1"/>
        <property type="gene ID" value="ENSXCOG00000001297.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158676"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000034">
        <property type="entry name" value="Ras suppressor protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="4">
        <location>
            <begin position="78"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="203"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="231" mass="25927" checksum="ECB51CAFD77358FE" modified="2018-12-05" version="1">MSKSLKKIVEESRDRSLPEVEMCDRGISNMLDIPGLFTLSHITQLVLSHNKLTGKMVPSCCWNGASCSFGISGKINANTQPSYFCFLATLRALYLSDNDFELLPPDIGKLTKLQILSLRDNDLVSLPREIGDLAQLKELHIQGNRLTVLPPELGNLDLTGPKQVFKAENNPWVTPIADQFQLGVSHVFEYVRSETYKYLYGRHMQANPEPPKKSSDKSRKISRKPLAAKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KPK3</accession>
    <name>A0A3B5KPK3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KPK3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002063"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002093.1">
        <property type="protein sequence ID" value="ENSXCOP00000002063.1"/>
        <property type="gene ID" value="ENSXCOG00000001656.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013285"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051843">
        <property type="entry name" value="CPA1_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31102">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31102:SF23">
        <property type="entry name" value="SI:DKEY-162B23.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="435"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="8">
        <location>
            <begin position="86"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="493" mass="52197" checksum="A427849FE6AB936B" modified="2018-12-05" version="1">AGENHSSYCKSYPPVVCCPLLRKFCPCPPRGLLASLITKVLLAAVLFGVIVSITEEECLPGGNLFGITVVFLCAIVGGKAVALIQLPTLPPFPPLLGMLLMGFLLRNIPVVTDSVYINFRWSASLRNIALAVILARAGLGLDPKALRKLKTVCIRVAAGPCLIEASTAALISHFVMGLPWKWGFVLGFVLSAVSPAVVVPSMLRLQKDGYGVEKGIPTLLMAAGSFDDILSITGFTTCLGMAFAEGSTLYNLLRGVLEVGGGMVVGILLGFLIQYFPSVDQKHLVMKRSFLVLGLSVFAVFGSNMAGFPGSGGLCTLVLSFLAGLGWGEEKARVGEVISWAWDVFQPLLFGLIGAEIRVNELEGLTVGLGISTILIAVMVRVLFTFVCVLHAGFNLKEKVFIALAWMPKATVQAAIGSTALDMARAKANEEQMKYGIDVLTVAVLAILLTAPVVSLIIGILGPRLLQGPKTPTCTAAGSEDDTETPVTFESTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KS05</accession>
    <name>A0A3B5KS05_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Laminin EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KS05"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000690"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000700.1">
        <property type="protein sequence ID" value="ENSXCOP00000000690.1"/>
        <property type="gene ID" value="ENSXCOG00000000595.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000271620"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000188">
        <property type="entry name" value="Laminin subunit gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000180">
        <property type="entry name" value="Netrin G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF270">
        <property type="entry name" value="LAMININ SUBUNIT GAMMA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 6" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Laminin EGF-like" evidence="7">
        <location>
            <begin position="75"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="75"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="126" mass="13473" checksum="78BE5CEA8EDA7C6D" modified="2018-12-05" version="1">PVAVLLHCCRSFRASSCLRPAADSRCECGGRARRCLRDAWGLRCLDCQGNTEGRHCERCKDGFYQQGAQLSCTPCSCNPTGSVSNTCDSRGRCSCKEGVSGDKCDLCPDGPMGAEGCARTERILGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KSM4</accession>
    <name>A0A3B5KSM4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KSM4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001482"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001505.1">
        <property type="protein sequence ID" value="ENSXCOP00000001482.1"/>
        <property type="gene ID" value="ENSXCOG00000001199.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234530"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF133">
        <property type="entry name" value="IMMUNE-TYPE RECEPTOR 5 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="144"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="277" mass="31716" checksum="6EE69E19FD5E5F7B" modified="2018-12-05" version="1">HNDDVKVISRIWAICSYNSQRRNYYFSILILQESKVAASKLNFCSTSGTTEYVASQSVLPQNSQFHMFLLHHILQLQLNHLFIFSKDITASDAGTYYCAVATHEELFFGNGTFLDVQGNTVGTSNYRVVQKKMVSDSNRPDNYVTLQCSILSDFENNSCSEDLSVFWFRSDKSHPDIIYTDGNRTNRCQKKIDDQTGRVYNFSRKISSSDDGTYYCAVATCGEILFGNGTKLEGCMILYFPLKNTFSTIILSRKNIFFVFSSQSKKTKWETLNFPYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KT04</accession>
    <name>A0A3B5KT04_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Serpin peptidase inhibitor, clade I (neuroserpin), member 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KT04"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001090"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001103.1">
        <property type="protein sequence ID" value="ENSXCOP00000001090.1"/>
        <property type="gene ID" value="ENSXCOG00000000894.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158168"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010975">
        <property type="term" value="P:regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001966">
        <property type="term" value="P:thigmotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF50">
        <property type="entry name" value="NEUROSERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serpin" evidence="2">
        <location>
            <begin position="2"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="338" mass="38409" checksum="94673C7BFE3BB38A" modified="2018-12-05" version="1">MNVCVSAVIHAGVEFSLLQNLTEALSDDDAHYVIRFANSLFLQEGISFNPDFLQLMKKYFQAEVETVDFSESPVVAEQINNWVENHTESKIRSLLSADDFSSITRLTLVNAVYFRGSWKNQFRPENTRTFSFSRDDGSEVQTLMMYQQGDFYYGEFSDGSQEAGGVYQVLEMPYEGEDMSMMIILPRQEVPLASLEPIIKAPLLEEWANNVKRQKVEVYLPRFKVEQKIDLRTTLQELGITKIFTDDADLSAMSDGKDLYIGKAVQKAYLEVTEEGSEGAVGSGMIALTRTLVLNQQVMADHPFFFVIRNRRTGSILFMGRVMTPEVVEPFDHDFDSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTQ4</accession>
    <name>A0A3B5KTQ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Troponin I2, fast skeletal type</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTQ4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001882"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001910.1">
        <property type="protein sequence ID" value="ENSXCOP00000001882.1"/>
        <property type="gene ID" value="ENSXCOG00000001528.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234588"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060048">
        <property type="term" value="P:cardiac muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003009">
        <property type="term" value="P:skeletal muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050875">
        <property type="entry name" value="Troponin_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738">
        <property type="entry name" value="TROPONIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738:SF31">
        <property type="entry name" value="TROPONIN I TYPE 2B (SKELETAL, FAST), TANDEM DUPLICATE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017286529" description="Troponin I2, fast skeletal type" evidence="6">
        <location>
            <begin position="23"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19644" checksum="BF9A07A68A5968A0" modified="2018-12-05" version="1">RNKTRLCVWWCFLLQSLVLQVAQTWLEKEKKERAEAKEAYMAENCPPSDTSGDQATLMELCKKIHAAIDKIDEERYDIEAKVQKADKEIEELKIKVVDLVGVKKPALKKVRMSADAMLKALLGSKHTVNMDLRANLKQVKKEVKEEPAEAVGDWRKNIEDKADRKKMFESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXW8</accession>
    <name>A0A3B5KXW8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Immunoglobulin C1-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027899586.1">
        <property type="nucleotide sequence ID" value="XM_028043785.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027899587.1">
        <property type="nucleotide sequence ID" value="XM_028043786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXW8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002476"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002510.1">
        <property type="protein sequence ID" value="ENSXCOP00000002476.1"/>
        <property type="gene ID" value="ENSXCOG00000001904.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114160884"/>
    <dbReference type="KEGG" id="xco:114160884"/>
    <dbReference type="CTD" id="567"/>
    <dbReference type="GeneTree" id="ENSGT00940000165013"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050160">
        <property type="entry name" value="MHC/Immunoglobulin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944:SF62">
        <property type="entry name" value="BETA-2-MICROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944">
        <property type="entry name" value="MHC CLASS II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017394730" description="Immunoglobulin C1-set domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Immunoglobulin C1-set" evidence="3">
        <location>
            <begin position="39"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="116" mass="12880" checksum="F270AD04E805718E" modified="2018-12-05" version="1">MKLLLCLAALVAVFCAVSCKTSPPKVLIYSRDPGEFGKENSLICHVSNFHPPDITIDLLCDGTTLPNPVQTDLAFKQDWHFHLTKSATFTPESGKTCICRVNHAGKSKDYNWEPNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYZ1</accession>
    <name>A0A3B5KYZ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WH1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KYZ1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000774"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000784.1">
        <property type="protein sequence ID" value="ENSXCOP00000000774.1"/>
        <property type="gene ID" value="ENSXCOG00000000658.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157376"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005522">
        <property type="term" value="F:profilin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008154">
        <property type="term" value="P:actin polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070358">
        <property type="term" value="P:actin polymerization-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000697">
        <property type="entry name" value="WH1/EVH1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202:SF1">
        <property type="entry name" value="PROTEIN ENABLED HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11202">
        <property type="entry name" value="SPROUTY-RELATED, EVH1 DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00568">
        <property type="entry name" value="WH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="97" mass="11381" checksum="FFC62E8A07CCF2FC" modified="2018-12-05" version="1">MVYDDANKKWVPAGGSTGFSRVHIYHHTGNNAFRVVGRKIQDHQVEWGHFYDILSDLYHVSSQIRKYCTAEEKVTLQQKYFCVDYAQYTLCSKLLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZE1</accession>
    <name>A0A3B5KZE1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ARVCF delta catenin family member a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the beta-catenin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZE1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003922"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003966.1">
        <property type="protein sequence ID" value="ENSXCOP00000003922.1"/>
        <property type="gene ID" value="ENSXCOG00000003081.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157027"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000007">
        <property type="entry name" value="ARVCF, delta catenin family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028435">
        <property type="entry name" value="Plakophilin/d_Catenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372">
        <property type="entry name" value="PLAKOPHILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372:SF5">
        <property type="entry name" value="SPLICING REGULATOR ARVCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ARM" evidence="10">
        <location>
            <begin position="282"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="10">
        <location>
            <begin position="325"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="10">
        <location>
            <begin position="585"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="31"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="50"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="758" mass="84607" checksum="B3688938FAD45444" modified="2018-12-05" version="1">KEANNENPTTADELDTPTSIASVMTSTNETVSIVSAEKASPEVKVEPSESAPNTTTSSTTIATAQPQPVLQPGEPHPVNPDSQNRVYTLPDAFRGMGGDACPGYGSLSRFSLHGYLPTKNFQPGTHYTLPFLRDSYASAGLSNQTEENRENPLDVKADLPAAGYHTLGMHQFRPITTMELLREPSRTRSGYDDAFMAQLEGNLNPFFQAMCRAQTLQLPHKRSSMVSLDSIRRDPRWRDPNLHEVISMLRHPMDPVKSNAAAYLQHLCYENDLIKQEVRQLNGVPILVELLDHPKAEVHRKACGALRNISYGKDHNNKMAIKSCDGIQALVRLLRKSSSMEVKELATGTLWNLSSHEPLKMTIINQGLQTLTDEIIIPHSGWRRRDSVDASKPLSAEWTTVFKNTTGCLRNVSSDGAEARQRLRECEGLVAALLHALQSAVVKKDADNKSVENCVCILRNLSYHVHKEIPGAERFQEPHAGHLMRTVGHQKKKNEPECCSGKRPKGWKNGFVERKYNTLDLPKCAELIKGLDLLYQPEVVRLYLSLLTCSHNHNTLEAAAGALQNLAAGHWAWSSYIRATVRKEKGLPVLVELLRSDVDKVVRAVAIALRNLAIDRRNKDLIGNYALRDLVANLPYGQQHPVKNLEGDTVVSILNTIYEIITDNPENARVLIQGHAVQKLVAINKSSQSTRETKAASHVLQTIWAYKELRHTLTKAGWNKSNFKVFLYILYERKHFIRAGRPAVGLMDNKPPPLDSWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZM1</accession>
    <name>A0A3B5KZM1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box only protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins and acts as a regulator of mTOR signaling.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO9).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZM1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001009"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001021.1">
        <property type="protein sequence ID" value="ENSXCOP00000001009.1"/>
        <property type="gene ID" value="ENSXCOG00000000821.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003354"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22123">
        <property type="entry name" value="F-box_FBXL12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12874:SF9">
        <property type="entry name" value="F-BOX ONLY PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12874">
        <property type="entry name" value="F-BOX ONLY PROTEIN 48-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017244202" description="F-box only protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="4">
        <location>
            <begin position="32"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="341"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="350"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU369085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="412" mass="46026" checksum="BC811F7605930BC3" modified="2018-12-05" version="1">MIFTRLWSIFIALCWAIRCSATKAENRGTNKCCNLDYFPENILIDVLSYLSVRELVRAGRVCKRWKRLVKDQRLWRTVDLTAWKGVTSRILWFLLRQYLGCGLRCLRLRGLLLSARGGTFLSESWLKALSTKCPRLSKLCLLHADLRSLPNCQILPRSLRVLELRGCELPRSFFNQSLPTSPGKGIASPSGIGVERLVLNNVPSFTDQHLQNLASWERLSRLELRDTFRVTANGLRSCAAKEGLSGLEGLSRLKYLEIGITGRQGYQLQMASLGLGAGWLGLEELSLGGKEVGPGLLCASRLKDLKCLHLWACTLSELQIVRSCRMLRGLRQLEFLDVTFQPRQSPPAEEGQEQEGGEEQQNEEGGTGGESNDENRTLDGTDPFPSLRRSLAGLLPSCSLVFTNCSVQINVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZM2</accession>
    <name>A0A3B5KZM2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="15">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5 15">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endosome</location>
            <location evidence="4">Multivesicular body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZM2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003076"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003113.1">
        <property type="protein sequence ID" value="ENSXCOP00000003076.1"/>
        <property type="gene ID" value="ENSXCOG00000002438.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156873"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019871">
        <property type="term" value="F:sodium channel inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010766">
        <property type="term" value="P:negative regulation of sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048814">
        <property type="term" value="P:regulation of dendrite morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000017">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000026">
        <property type="entry name" value="E3 ubiquitin-protein ligase HACE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000006">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000008">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000069">
        <property type="entry name" value="Neural precursor cell expressed, developmentally down-regulated 4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024928">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF441">
        <property type="entry name" value="HECT-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001569">
        <property type="entry name" value="E3_ub_ligase_SMURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12 15 17" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="WW" evidence="19">
        <location>
            <begin position="68"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="19">
        <location>
            <begin position="235"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="19">
        <location>
            <begin position="338"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="19">
        <location>
            <begin position="389"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="20">
        <location>
            <begin position="481"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="33"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="194"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="272"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="208"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="287"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="316"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="16 17">
        <location>
            <position position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR001569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001569-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="816" mass="94863" checksum="3BB18F9F03D40AAA" modified="2018-12-05" version="1">MERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKQGGQEEEAGEMREEAEGWDESADSGSQRPQQLLPPLPSGWEEKVDNLGRTYYVNHNNRTTQWKRPSNMDVISEIESDNQQRQINQEAHRVFRARRHISEDLENERMDSRDMDNVREQAEDILIVLTHTCTQPQLSRVTRSEKHVFSCFLFFSPSVLGAHHRRGSQRQLGPLSNRLRSSSMTDGVSDQAQSPSAASYTLTTPGLPPGWEERKDGKGRTYYVNHNNRTTTWTRPIVQLTEDGASTSAPQVRRPRSLSSPTVTLSTPLEGANNIQVRRAVKDTLSNPQSPQLSPYSSPKSQHKTQQSFLPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRNKNSMEPGELGPLPPGWEERVHTDGRTFYIDHNTKTTQWEDPRLQSPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVFHGKLLDGFFIRPFYKMMLGKQISLKDMESVDSEYYNSLKWILENDPTELDLRFCIDEDNFGQTYQVDLKPSGSDMVVTNENKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVDVNDWRQHTVYKNGYCPNHPVIQWFWKVVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGTPDKLPRAHTCFNRLDLPSYESFEDLREKLLMAVENAQGFEGVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L0T3</accession>
    <name>A0A3B5L0T3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Hydroxylysine kinase</fullName>
            <ecNumber evidence="8">2.7.1.81</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the GTP-dependent phosphorylation of 5-hydroxy-L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(5R)-5-hydroxy-L-lysine + GTP = (5R)-5-phosphooxy-L-lysine + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19049"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57882"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58357"/>
            <dbReference type="EC" id="2.7.1.81"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aminoglycoside phosphotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.81" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L0T3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004412"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004465.1">
        <property type="protein sequence ID" value="ENSXCOP00000004412.1"/>
        <property type="gene ID" value="ENSXCOG00000003480.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011314"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047992">
        <property type="term" value="F:hydroxylysine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000549">
        <property type="entry name" value="hydroxylysine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1200.10:FF:000007">
        <property type="entry name" value="hydroxylysine kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050249">
        <property type="entry name" value="Pseudomonas-type_ThrB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21064">
        <property type="entry name" value="AMINOGLYCOSIDE PHOSPHOTRANSFERASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21064:SF1">
        <property type="entry name" value="HYDROXYLYSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="10">
        <location>
            <begin position="29"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="358" mass="40824" checksum="18D47CC97DC847C2" modified="2018-12-05" version="1">MSVKNAQPNFSPSQVSEIMKRLYNLTASEIRPLPSYDDQNLYVAPTEGGEYVLKIMNTEDSKNPTLIELQTHAMSFLYQNGLPAQTAMPTTSGQLMSLEEIDCGYGCQKYLVRLLTYLPGVTVSKVPLTPKLLYETGRMAARMDEVLQKMEHPHLSLLERENFIWSLSNICLLEEYMSVLDGDPLQEVVKSVIDQYKNSVIPRRSNFRKCINHGDFNDLNILMQPDDNHGYKISGILDFGDMNSGFYINELAITIMYMMIEHPDPIEVGGPVLAGWESVIPLNKDEKDCLYVLVLSRFCQSLVMARYSVMLHPENGEYLMITSRKGVKILQKVWELGKEHVEKVWFDHAAQQLKSKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L189</accession>
    <name>A0A3B5L189_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L189"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001594.1">
        <property type="protein sequence ID" value="ENSXCOP00000001569.1"/>
        <property type="gene ID" value="ENSXCOG00000001268.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="74" mass="8648" checksum="BE80BE3161A0B7AB" modified="2018-12-05" version="1">MQKKLDHLPFCFLVHRHTDTLKLLLVNFGLHTFFFQILSILLMYSLPNVENSKQVGGGIRALIQRRKGKMLWVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2N9</accession>
    <name>A0A3B5L2N9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pentraxin (PTX) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027890995.1">
        <property type="nucleotide sequence ID" value="XM_028035194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2N9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004131"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004176.1">
        <property type="protein sequence ID" value="ENSXCOP00000004131.1"/>
        <property type="gene ID" value="ENSXCOG00000003240.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114155348"/>
    <dbReference type="KEGG" id="xco:114155348"/>
    <dbReference type="CTD" id="556906"/>
    <dbReference type="GeneTree" id="ENSGT01060000248591"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00152">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000012">
        <property type="entry name" value="neuronal pentraxin receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051360">
        <property type="entry name" value="Neuronal_Pentraxin_Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030476">
        <property type="entry name" value="Pentaxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277:SF1">
        <property type="entry name" value="NEURONAL PENTRAXIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277">
        <property type="entry name" value="PENTRAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00895">
        <property type="entry name" value="PENTAXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00159">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00289">
        <property type="entry name" value="PTX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51828">
        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017441188" description="Pentraxin (PTX) domain-containing protein" evidence="9">
        <location>
            <begin position="18"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Pentraxin (PTX)" evidence="10">
        <location>
            <begin position="224"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="95"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="124"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="433" mass="47566" checksum="E34844434B0E2B2E" modified="2018-12-05" version="1">MLSLWCGFLSFCVLICAQPDDGKRYICRAVAVSGDAGCPVTLLPDLSAGGQEEELRNTVMQLRETIVQQKDTISKQLGTINELTTKLSLCASAASDRKSGKGGTWGKDKQNTMGDVPTDPNDSVDSLGKTMQGLKERLENLEQQQMRANLSGASFPSELRELLQRRLGELEKQLLKKVSSLEEEKSQLSNATAAYRLKTESTLNALVERISELEKGAGDFKSPEQFKLSLPQRTNYLYGRITKSLPEMYAFTLCMWIKSSASPGVGTPFSYGVPGQANEIVLIEWGNNPIELLINDKVAQLPLEVRDGKWHHICISWTTRDGQWDAYQDGDKLGAGDNLAAWHPIKPGGVIILGQEQDVVGGRFDAGQAFVGELSQVNIWDRVLKPAEIRSMANCSAYLAGNVVAWLPGSVEVFGRGASKRPLEVCQERLPNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L350</accession>
    <name>A0A3B5L350_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BPTI/Kunitz inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L350"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005232"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005293.1">
        <property type="protein sequence ID" value="ENSXCOP00000005232.1"/>
        <property type="gene ID" value="ENSXCOG00000004094.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156891"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020901">
        <property type="entry name" value="Prtase_inh_Kunz-CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF281">
        <property type="entry name" value="PAPILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19030">
        <property type="entry name" value="TSP1_ADAMTS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00759">
        <property type="entry name" value="BASICPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00280">
        <property type="entry name" value="BPTI_KUNITZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="6">
        <location>
            <begin position="632"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="524"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="532"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="16"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="20"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="688" mass="76099" checksum="B39591D561DAF07B" modified="2025-10-08" version="2">MPSVDYWDSWGPYGECSRSCGGGVTMRTRRCVTQTDGGHNCVGPDKSYRICNIQDCPEGSRDFREEQCSQFDGTDFQGKRYKWLPYYGENPCELNCMPRGENFYYRHRSSVADGTPCHPGRRDICVGGVCKRLGCDNMLESSQQEDPCLQCGGSGQSCHLVKNTFNARNLYNQMFIIPVGATTISIRETTATRNYLIKNLRGEYYLNGHWVIEFSRATPIAGTMLYYQRGAEGENVPETIIGRGPTTEPLVVELISQETNHGIEYEYYLPNGRSRQGYYWSSGSWSSCSKECGSYQSRLVFCSIDNEAYPDYLCASLPRPQSNRTCNPHPCPQTQMAYLYPPQSWRTGEWNACSVTCGGGSQLRSVQCILHDASGPQVVEDVVCAAYVDAPPSLRTCNMHKCAEYRCSVSCGSGDQTRDVTCVGSGGTMLEEASCRTLLRPPSVQPCEMPACPKQIHWHVGAWGLLQCSRSCGSGSRERQVICSDQERNLYHVDQCNAQPKPSTMERCNTQPCFSPQVVPSIQNPRGHDRAQTSFQPPTQRNTVRDPHDFSPALHCSQSIYGCCPDGRTSARGPQSQGCPYGCCQDGVRAAQGPNREGCNAVECRTTTYGCCYDRKTPAGGPNGEGCPNPPNHLPRAAGSCSSWVARYHFDVVTSKCAHFWYGGCHGNSNNFMSRAECQRACQVSAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L359</accession>
    <name>A0A3B5L359_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Voltage-gated potassium channel regulatory subunit KCNF1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Potassium voltage-gated channel subfamily F member 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Voltage-gated potassium channel subunit Kv5.1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">KCNF1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Regulatory alpha-subunit of the voltage-gated potassium (Kv) channel which, when coassembled with KCNB1 or KCNB2, can modulate their expression and their gating kinetics by acting on deactivation upon repolarization and inactivation during maintained depolarization. Accelerates inactivation but has relatively little effect on deactivation. Coexpression with KCNB1 or KCNB2 markedly slows inactivation. Each modulatory subunit has its own specific properties of regulation, and can lead to extensive inhibitions, to large changes in kinetics, and/or to large shifts in the voltage dependencies of the inactivation process. The gating kinetics depends on the nature and stoichiometry of the associated regulatory sunbunit. Fails to produce a potassium current when expressed alone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Heterotetramer with KCNB1 or KCNB2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the potassium channel family. F (TC 1.A.1.2) subfamily. Kv5.1/KCNF1 sub-subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L359"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004800"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004858.1">
        <property type="protein sequence ID" value="ENSXCOP00000004800.1"/>
        <property type="gene ID" value="ENSXCOG00000003771.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160213"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18381">
        <property type="entry name" value="BTB_POZ_Kv5_KCNF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000046">
        <property type="entry name" value="Potassium voltage-gated channel subfamily F member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000072">
        <property type="entry name" value="Potassium voltage-gated channel subfamily F member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000005">
        <property type="entry name" value="potassium voltage-gated channel subfamily G member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003971">
        <property type="entry name" value="K_chnl_volt-dep_Kv5/Kv9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048010">
        <property type="entry name" value="KCNF1-like_BTB_POZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF171">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY F MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01494">
        <property type="entry name" value="KV9CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="173"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="382"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel tetramerisation-type BTB" evidence="22">
        <location>
            <begin position="19"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="21">
        <location>
            <begin position="177"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="467"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="478"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00060051"/>
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    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060851"/>
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    <evidence type="ECO:0000256" key="15">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00071451"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00076730"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02214"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004800.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="495" mass="55521" checksum="3ED830CA28B7C285" modified="2018-12-05" version="1">MGYGQILHQHGKEEDQAEIVVNIGGVKQVLYGDVLNRFPETRLAELVDCSLKSPEEISSLCDDYDPDTGEFYFDRDPEAFKCIVELYYYGEVHMKRGICPICFMKEMDFWKIGADFLDECCKSNLKEVQDELAEIAERVRTILVDREGDPSAGGCQRFQASLWRLMEKPESSVPAHVIAILSFIFILVSSVVMCVGTIPELQVEDSDGNLTEHPTLEVIETVCIGWFTIEYLLRLISSPNKIKFVLSFMNIIDFMAIMPFYVVLILTSFGAGVMELANVQQAVQALRIMRIARIFKLARHSSGLQTLTSALKSSLKELGLLLMYMGVGVFLFSALGYTMEQNHPDTLFTSIPQSFWWAVITMTTVGYGDVYPKTTLGRCNAAISFLCGVIAIALPIHPIINNFVLFYNKQQVLETAAKHEIELMALRSTEGELRAAPEHREHVCGSRVWDTSMSSCHSDTFLPLLKDTRGGPSMQTPSMETSFESTAEASEYFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L3K0</accession>
    <name>A0A3B5L3K0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cadherin-23</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Otocadherin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells. CDH23 is required for establishing and/or maintaining the proper organization of the stereocilia bundle of hair cells in the cochlea and the vestibule during late embryonic/early postnatal development. It is part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000004426"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004479.1">
        <property type="protein sequence ID" value="ENSXCOP00000004426.1"/>
        <property type="gene ID" value="ENSXCOG00000003481.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155245"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000141">
        <property type="entry name" value="Cadherin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000142">
        <property type="entry name" value="Cadherin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000147">
        <property type="entry name" value="Cadherin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000173">
        <property type="entry name" value="Cadherin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000228">
        <property type="entry name" value="Cadherin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000098">
        <property type="entry name" value="cadherin-23 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000104">
        <property type="entry name" value="cadherin-23 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="cadherin-23 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000135">
        <property type="entry name" value="cadherin-23 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000146">
        <property type="entry name" value="cadherin-23 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="cadherin-23 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000156">
        <property type="entry name" value="cadherin-23 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="cadherin-23 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Dachsous cadherin-related 1b"/>
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    </dbReference>
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        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protocadherin 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000100">
        <property type="entry name" value="protocadherin Fat 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000060">
        <property type="entry name" value="Putative cadherin-23"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050971">
        <property type="entry name" value="Cadherin-domain_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056989">
        <property type="entry name" value="PCDH15_12th_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24025:SF18">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24025">
        <property type="entry name" value="DESMOGLEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23206">
        <property type="entry name" value="PCDH15_12th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="27"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="27"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="27"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1009">Hearing</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 19" id="KW-0732">Signal</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017274559" description="Cadherin-23" evidence="19">
        <location>
            <begin position="32"/>
            <end position="3296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="3040"/>
            <end position="3063"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="36"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
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            <begin position="120"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="224"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="336"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="446"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="547"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="657"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="764"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="874"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="979"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="1121"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="1179"/>
            <end position="1287"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="1283"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="1388"/>
            <end position="1495"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="1497"/>
            <end position="1602"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="1619"/>
            <end position="1712"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
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            <begin position="1727"/>
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        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="1820"/>
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        </location>
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    <feature type="domain" description="Cadherin" evidence="20">
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            <begin position="1928"/>
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    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="2038"/>
            <end position="2142"/>
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    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="2143"/>
            <end position="2261"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="2265"/>
            <end position="2370"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="2371"/>
            <end position="2477"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="2478"/>
            <end position="2579"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="2582"/>
            <end position="2691"/>
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    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="2698"/>
            <end position="2819"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="20">
        <location>
            <begin position="2820"/>
            <end position="2940"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="3259"/>
            <end position="3296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="3296" mass="361874" checksum="FB36E836CF78C73A" modified="2018-12-05" version="1">FHMLLSACYQIFHLFFLYFTNGNILLPYTTSDPCPSPAGSSVAQLKAMDPEGEPLIYGVSGEEAMKYFSVNKETGVVWLRQQLDRETKSEMQVEFYVSDLQEVRVNIQIGDVNDNAPNFHGQPYTVHIPENTPVGRSIFMVNATDPDQGTGGSVLFSFQPPSSFFAIDGARGTVTVTKTLDYETTAAYQLTVNATDQDKVRPLSKLANLAITITDVQDMDPIFTNLPYSTNIEEDIPLGYEVRKITAIDQDLGQPRGIGYTIVSGNTNSVFALDYISGSLTVNGQLDRENPLYSAGFTLRVKATELKDDRSPSDATVMTTFTILLIDKNDNAPKFNSSEYRVRITELAQVGFALPLFIQVEDKDEGVNSMFQVFLTGNNSEYFTISPTAVQGHADIRVRVALPLDYEQIRSFSFSLYANESMSDHVGFARVFIELINENDNRPIFSKPLYNISLPENTPPGTSLLQILATDGDVGPFGIVRYYFSDDPDQFSLDAETGWVILQASLDYELMRRFTLTVLARDGGGEETTGRIRVNVLDINDNTPLFQKEAYVGSVRENEQAVQSVARIRATDEDSPPNNFLTYTIISASAFPQYFSIIMVEGYAVISVTRPLDYEMVPKGLIYLTVMAKDGGNPPLNSTVSVTVEVIDENDNPPEFSKPSYIVKIPENLNAGATVLVVNATDLDASREFGQASLIYSLEGSSQFRLNSRSGEITTTALLDREQKSEYILIVRAVDGGVGPQQKTGIATVNITILDINDNAPTWRDEPYEANVVEMSPINTDVISVLAVDPDNGENGTVVYSISPANPFYTIDSRTGKIRTSGVTLDRESTRDTELMRTIIVSAADRGTPPLRTSASTTVSVNLLDLNDNDPTFLNLPSVAEVPEGLPIGNSFFRVQVVDPDKDANGRITMALQLCVPRLDFYLNTSTGVLTSTAVLDREQIGQYQLRIIAHDAGEFPRTSTSTLTVSVLDVNDETPTFSPRLYNVSLMENVPRDYVVARLVCFDKDAGLNAELSYFITGGNQDGKFSVGFRDGIVRTVVGLDRETQAAYTLVIEAIDNGPADSRRTGTATVSVEVQDVNDNRPIFLQNSYETSILETVPIGTSILQVGRAWTESAAGNHGINRQSGQVTRGVRALDRETSSSHVLEVEAFNSDEGSMRSSVRVIIYVEDANDEVPVFTQQQYNRLGLRETAGIGTSVIVVRATDPDTGDGGAVAYSIVSGSDRKFEVDVSTGLVTTVDYLDYETKTSYLMNLSATDQAPPFHRGFCTVYVTLLNELDEAVAFFSPGYEASLRENIATGTEVVQVQARSADNLNQLTYRFDPDTSPAALALFKIDSVTGRITVTGLLDREKGDLYTLTVVADNGGPKKDSTVVSITILDENDNSPVFDITSDTSVSIPENTAMGKRVAVVLAKDSDAGLNGLVNFTLVAGNMEDVFKIKTVNNSYGEVYVNAALDRESVDRYLLKVRATDNGSPTRYTDHSLTVNILDVNDNAPVIESQRGYNVSVNENVGGGTSVLRVIATDRDIGPNAMLFYYITAGNQDLTFRMDRMTGEMVTRPAPPDRERQQEYRLNVTVEDDGMPPLSTSTTVYIRIVDENDNAPDFPEEEYVTVLSEGPDTVGATIATVTAIDPDEGLNGTLRYAITQGNLIQTFNINSITGRITAVKELDYEISKGHYALVVTATDQCPKPALRLTSSTTVSVNVLDMNDVTPTFPRQYEGPFEVTEGQPGPRVWTLRASDEDSGLNGKVEYSITGGDNQNEFMISPVEGELRVRRDVELDRETTAFYNITVTARDLGTPSRNSSVVVGVYVLDINDNDPVILNLPYNTSVSEGAAIHTSVARVQARDADSGRNALLTYNITAGNPSGAFYINDTSGVVQVNRPLDRELVAEYTLTITVKDNPENPRIARRDSDFLVITILDENDNRPVFTRTSYRAEITENSPAGSTVTVLNGPVLAEDKDIGPNAVVKYRLLGPRVDLFIVDSNTGVLLVRQGATLDREAFQDPRVELILVGEDVGGLNNSVPLTVTILDQNDNPPVFSPSSFSVRLPENSPTGVVVTQLSATDADSGANGWLMYRLESGAQDRFVVDPISGAVLVGNTTLDREERGSYRLVVMATDRGTPPLSGTATLTVILDDINDSRPRFIEPITMINVNESTPPGVVVATLTAEDPDLNPRLEYYIISVEAKDDGNNPVLGLQNSFGIDLHTGAVFVRNPLNRELVATFEIIVSVHDNASQVIDKSGSVPNARLTINVLDVNDNAPRFRPFGVTNFTEQILEGAQPGTTLLSVSAVDPDKGPNGQVIYQLLHLPRGGYVRLEDSSMGKIVANQTVDFEQVQWLNFTIRAQDHGTPPRSAELPVYLRIVDVNDNNPVFLQPSYQEPVFENVDLGTTIARVRATDADSGLFAVIEYSLVDGEGKFGIKPSTGEIYILSPLDRETKDHYTLTAVARDNPGGSPNNRRENSVQVLVTVLDVNDYRPRFTKRVYNTSVFENEPSGTSVITISAIDLDEGENAALIYSILGPNLDAFSLDPITGLVRSRRLLQSSERFNFTVVATDQGRPPLWGTADLIITVIDVNDNRPVFVRPANGTIIHISEEQPPGLPVYEVHATDSDEGVNGEVRYAFLQTGAGNRDWENFHIDTMSGVITTTVKLDREKQALLSLIIVARDMGQPVPYETTQPLQVALLDIDDNEPVFLKPPRGSLPYQKLTVPEHSPSGTVVGNVTRAVDADEGSNAIVYYFITGGNSDANFGLSLDGELKVLRDLDREEIPAYSIIIKASSNRSWTPPKGQRSLRAKALNPALDPSLLEVRIELEDINDQTPRFAKAEYTAGVAANAKVGSDLIRLVATDNDIGNNSVVQYHIVTIRYFQTQSNGSEDVGNIFTIGLTDGMIRTYDLFTAYNPGYFQLEILACDLAGHSSTTNVNIYILRDDQRVKIVFNEIPDWVRENQDNFISLLSNITGAIVNLDDIQFHVDKKGRVSYSQTDMLIHVVNNQTNTILDVERVIQMIDENKEQLRSLFRTYNVVDVQPAIGDKLPDDISTLQLVIIILAVLLCLAGILFVTMNWHYRRVHQRKLKAIVAGSTGNQGLMDILDMPNTNKYSFEGANPVWMDPFCRNLELAAQADHEDDLPENLSEITDLWNSPARTHGTFGREPQARPEDDRYLRAAIQEYDNIAKLGQIMREGPIKLIQSDLDDDEDENLDLAGGRGTLRFKHKLPVELKGPEGVHVVHGSTGTLLTSDINSLPEDDQRALARSLEALNADGGLYSERNARTESAKSTPMHRHKDGDTLSESPLEITEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4Z0</accession>
    <name>A0A3B5L4Z0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane channel-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TMC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L4Z0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007388"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007483.1">
        <property type="protein sequence ID" value="ENSXCOP00000007388.1"/>
        <property type="gene ID" value="ENSXCOG00000005695.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244942"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050910">
        <property type="term" value="P:detection of mechanical stimulus involved in sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060005">
        <property type="term" value="P:vestibular reflex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038900">
        <property type="entry name" value="TMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012496">
        <property type="entry name" value="TMC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302:SF17">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07810">
        <property type="entry name" value="TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="440"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="514"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="657"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="706"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="763"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="domain" description="TMC" evidence="8">
        <location>
            <begin position="586"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="805"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="103"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="805"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="843"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="886"/>
            <end position="897"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU310713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="913" mass="102099" checksum="C50BDDAFA28597A1" modified="2018-12-05" version="1">RGGGGRGRGRGRGRGKKAGSDEEEDDEDEAPKGRGRGGNRRKPGNKRGGGKNNGDDDDSEDESPKKKRGGAANRKKGGKAQDSDEDDSDDGKGKRGKKGGKKGGKEEESGKGKKGDAKGKDKGKDKGKDKDDDKKKKKKGDSSSDSNSNSDEEEESMSEGEMARLMEEVEEKKKLIATIRNKPWRMKRRLVLLKEAQQFVDKFEGALGKGKGRKWYAYKVMMTKKWIKFQRDFENFRTACIPWERKIKEVESHFGSSVASYFIFLRWMYGMNLVLFGLTFGLVVIPEVLMGLPYGSIPRKTVPRAEQDTAQDYSVLMDFNGYCKYSVLFYGYYDDKRTIGPLKFRLPLSYLLVGIGSFGYSLMLVIRTMAKNADVGGGDGEEGEFTFAWKMFTSWDYLIGNAETADNKYASITTSFKESIVDEQENQKDENIHLRRFLRVLANFLITCSLGGSGYLIYFVVKRSQEFANRNDLSWYEKNELEIIMSLLGLVCPPLFETIAELEDYHPRIALKWQLGRIFALFLGNLYTFLFALFDEVNSKLDEEQSIKNASIWAMKEYYANYSRLLNDSDATPPPMSPADVIRGPCWETAVGIEFVKLTVSDIQVTYLTILIGDFARAVIVRFLNYCWCWDLEAGFPSYGEFDISGNVLGLVFNQGMIWMGAFYAPGLVGINVLRLLSSMYYQCWAVMATNVPHERVFKASKSNNFYMGLLLLILFLSLLPVVYTIMTLPPSFDCGPFSGRAKMFDVIMETITLDLPAFMGTLFSYAANPGLIIPAVLLMVLAIYYLNSVSEAYQNSNMELKKKMQMARDEEKNRRNNKDSTNQVMKDLEDLLPNRSLSPPPEPEKPPEPEAKPTKTKPGSAGKGVNLQKDVALASSNPNVRGPVDRPPGPRGPGPGPGAGRGRGRGRGAPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5F6</accession>
    <name>A0A3B5L5F6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="6">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5F6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007543"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007639.1">
        <property type="protein sequence ID" value="ENSXCOP00000007543.1"/>
        <property type="gene ID" value="ENSXCOG00000005811.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156070"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="protein phosphatase 1B isoform X1"/>
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        <property type="entry name" value="PPM-type phosphatase domain"/>
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        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PP2C-like"/>
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    </dbReference>
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        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0519">Myristate</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 18" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="20">
        <location>
            <begin position="23"/>
            <end position="290"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="16"/>
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    <evidence type="ECO:0000256" key="1">
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    </evidence>
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        <source>
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        </source>
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    </evidence>
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        </source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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    <evidence type="ECO:0000313" key="21">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="331" mass="36448" checksum="5F6C7D178C7FB7D6" modified="2018-12-05" version="1">MGAFLDKPKTEKHSAHGDGNGLRYGLSSMQGWRVEMEDAHTAVVGLPHGLTDWSFFAVYDGHAGSRVANYCSGHLLEHILSGGTEFGSGPGSVEGVKDGIRSGFLNIDEYMRNFSDLRQGLDRSGSTAVCVLLSPTHLYFINCGDSRSVLCRDGKVGFSTQDHKPCNPREKERIQNAGGSVMIQRVNGSLAVSRALGDYDYKCVDGKGPTEQLVSPEPEVCVLERASEGDEFVVLACDGIWDVMSNEELCDFVRSRLLVCEDLEKICNSVVDTCLHKGSRDNMSVVLVCLPGAPKISEEAMKKEEELDKYLETRVEGQHFKHTHPQRRTLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6F1</accession>
    <name>A0A3B5L6F1_9TELE</name>
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        </recommendedName>
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        <name type="scientific">Xiphophorus couchianus</name>
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        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="336" mass="39384" checksum="1CD12351FADC36B1" modified="2018-12-05" version="1">GALQVKKQFIRLLNRTKWKLKRAEFEYKQHVMNSATITSNFPKHKEKVAIKKLHRPFQSEIFAKRAYRELRLLKHMKHENVIGLQNVFTPACSLDDFQDFYLVMPYMYTDLSKVRGQLSEDKVQFLVYQMLCGLRYIHKAGIIHRDLKPGNLAVNQDCELKILDFGLARSADAEMTGYVVTRWYRAPEVILNWMHYTQTVDIWSVGCIMAEMINGKTLFKGKDYMDQLSQIMKVCGVPGQEFIQKLESPEARSYIKGLPRHPRKDFSTLFPRASEQGVNMDAFNIFFFLQTKMWLCRRKPNRPPFSLTGLCYKEVNSFVPFPRRDSKRKNTLTMSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6I7</accession>
    <name>A0A3B5L6I7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Castor zinc finger 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSXCOG00000004684.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CASZ1"/>
    </dbReference>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12451">
        <property type="entry name" value="TRANSCRIPTION FACTOR CASTOR PROTEIN MING -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12451:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN CASTOR HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="583"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="700"/>
            <end position="729"/>
        </location>
    </feature>
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        <location>
            <begin position="60"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="116"/>
            <end position="205"/>
        </location>
    </feature>
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        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
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        <location>
            <begin position="767"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1306"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
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        <location>
            <begin position="118"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="174"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="326"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="779"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="802"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1314"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1327"/>
            <end position="1352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1375" mass="152172" checksum="51BC0CE7D91727BE" modified="2018-12-05" version="1">MSITVFYSRCISIFLPVFPPAERSLCTDTTSGKPKMAAKRKGGLKLNAICAKLSRQVVYDSSSQNAEGDQSVAENSERGSSYYDDNETNFPESLSLSQSLEEDQKRREAIEKWVNGEYWEEPPAPDEEQEHELRVSNDEDSPPEGVYMVQPKGCSDEEDNGEEAEATTGSQDGSYHDDKETDERPPKDNSYMPPSEGQSREASALRDYAVNTMNKFLGMFGYDDHQVRDEVTKKIGFDAVKATSDPSSLSNAEDSRSPRFSKYEEYIRKLKAGETLPWPVHASPPKPDDLNSKLTQEKSASMLQSSGGLPGVEGQIYHSSLDHKQSGATQLGNSQPPNSSHVQNMASRASKYDFFIQKLKMGESLQQQNGNAYKRPSKYDLENVKFLHLFKPGEGNPDMGGAIAFKTGKVGRPSKYDIRTIQKLIPGNPETSLIPNVLATAPGNPGAPGAGHISFNAADYMKSSFSKTDSITTGTVSSVKNGLPPDKPASDDINLYQKYIARFSGSQHCGHVHCAYQYREHYHCMDPECNYQRFTSKQDVIRHYNMHKKRDNSLQHGFMRFSPLDDCSVYYHGCHLNGKSTHYHCMQVGCSKVYTSTSDVMTHENFHKKNAQLINDGFQRFRATEDCGTVGCQFYGQKTTHFHCRRPGCTFTFKNKCDIEKHKSYHIKDDAYAKDGFKKFYKYEECKYEGCVYSKATNHFHCIRSGCGFTFTSTSQMTSHKRKHERRHIRSSGVMGLSSTFLAPKDEQEESSNDDLMDFSAISSKNSSLSASPTTQQSATAPHLLSTPTTATASSMSGHNLKPTQSLPGAGQRMSSLLTQNLPSNMPVALALSNSALASSNPFFPLLPRLPLQPPPTAAGLITAVSSGAHSMPTDSLTQGGSSVGGDAAMAPTPTSFASSSIMEKISASKGLISPMMARLAAAALKPLNNIDTGRSNESNGHPVPGNTSLLSSLMNKVRSIHQTPFIPRQISGTRRGLFLNESSSLLGKQYIYASFFFYSPIQRFDTDDFCEAQCDFLHKVHFHCLVEDCGALFSTVDGAIKHSNFHLRATLKVKPESPFSDGKEPGEAAPLQPVAPVSIASNPSMDVANLTSSGGYSSPPPSLLAWKQLTGSIPQMPASMPNLPANSHDPCLATDCKYSNKFHFHCLFGNCKYVCKTSGKAESHCLDHINPNNNLVNVRDQFSYYSLQCLCPNQHCEFRMRGHYHCLRPGCFFVTNITTKLPWHIKKHEKAERRAANGFKYFTKREECGRLGCKYNQVNSHFHCIREGCQFSFLLKHQMTSHARKHMRRMLGKNFDRVPSQVRNTTKRKTFKPSSPLGLSSESSNQDRSCTSTPVGNDSSPAGEGTVNSKKPTQTFGLTHVNLSHLLLICVMIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6I9</accession>
    <name>A0A3B5L6I9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6I9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003394"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003432.1">
        <property type="protein sequence ID" value="ENSXCOP00000003394.1"/>
        <property type="gene ID" value="ENSXCOG00000002677.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167095"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003531">
        <property type="entry name" value="Hempt_rcpt_S_F1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF7">
        <property type="entry name" value="INTERLEUKIN-21 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01355">
        <property type="entry name" value="HEMATOPO_REC_S_F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="8">
        <location>
            <begin position="126"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="400" mass="45216" checksum="3689A69561E0B21A" modified="2018-12-05" version="1">MGLRSLRLTETDQNSRLPSFPDADYNLDCVNDYLFTLNCSLNTSPAASNKTYWLKVTSDLDQTEYKCLLKNTTAGFFCSLDRSLMPDDDETERFSDDEYFEISLCENHKEICELLEENYEPELHIKPSAPCCLEVSHNSTRHHFTWKSTYESFTDTALGEYLMYQLQLKARNISITTTHFVEDQHFEPGTEYAAQVRSSPNQRHYKGQWSDWSSAVQWRTEPAENGFIFNLPVTFGLLCALVVLVLVCYGSHSTFIPTPAPYFHSLYKDCQGDFKSWVVTQENAADVLKPEEALRIDSLVECMPVQDFQFHQYPSVCGCAAPLLDQRGSVNSLSGYCRSCSPAQDSGCWLCSDTSLEKESSWYCNQYCTLSCLQQAGCAAAGCHGDLKAHPRFSDAGMEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L775</accession>
    <name>A0A3B5L775_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Exocyst complex component 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Exocyst complex component Exo70</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the EXO70 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L775"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006290"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006365.1">
        <property type="protein sequence ID" value="ENSXCOP00000006290.1"/>
        <property type="gene ID" value="ENSXCOG00000004864.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003595"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.170">
        <property type="entry name" value="Exocyst complex component Exo70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004140">
        <property type="entry name" value="Exo70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046364">
        <property type="entry name" value="Exo70_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542:SF41">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12542">
        <property type="entry name" value="EXOCYST COMPLEX PROTEIN EXO70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03081">
        <property type="entry name" value="Exo70_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20669">
        <property type="entry name" value="Exo70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex subunit Exo70 C-terminal" evidence="7">
        <location>
            <begin position="283"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="652" mass="74872" checksum="080055007085F488" modified="2018-12-05" version="1">MIPTEDASARKRETEEKLKQEQETLSFIRENLEKSDQLTKGMVSILSSFESRLMQLENSIIPVHKQTENLQRLQENVDKTLSCLDHVISYYHVAKDTDRIIREGPSGRLDEYLACIARIQKAVEYFQDNNPDSPELNTVKARFEKGKELLEAEFRSLLTRYSKPVPPILILDAISVDEDIELQEEVVLEHLPEAVLQDIICISGWLVEYGRNQDFMNVYFQIRSSQLDRSIKGLKDHFRKNSASSGILYSPAVQTKRKDTPTKKAPKRPGKDDVLDIEIDSYIHCISAFVKLAQSEYALLTEIIPEHHQKKTFDSLIQEALDNLMLEGDNIVSAARRAIMRHDYSAVLTIFPILRHLKMNKSEFDSTLQGTAASTKNKLPTLITSMETIGAKALEEFADSIKNDPDKEYNMPKDGTVHELTSNAILFLQQLLDFHETAGAMLASQETSTASSSTSDFNKRLLSSYICKVLGNLQLNLLSKSKVYEDSALSAIFLHNNYNYILKSLEKSELIQLVTVTQRKAESSYRELIKQQIDIYQRSWLKVLEHLTDKNMPIFQPGAKLKDKERQVIKDKFKGFNDGLEELCKIQKGWAIPDKEQRDYIRQSQRKIVSHAYRAFVQRCANISFTKNPEKYHKYRPEEVEEMIEKLFDTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8K9</accession>
    <name>A0A3B5L8K9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein 44</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8K9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004179.1">
        <property type="protein sequence ID" value="ENSXCOP00000004134.1"/>
        <property type="gene ID" value="ENSXCOG00000003246.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000178708"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF53">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="245"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="302"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="325" mass="35757" checksum="DE12D26CF59B8AE7" modified="2018-12-05" version="1">MDGGVSTAEDELRGSSDHFLTSLGAFWLHSVASCGSPDAAHLCVPAGLTLLSAVLLLLSSLFQCYHFRIVCRKCKLRGEYSKETTIVFYSFLGNLCSTVGAVLARTILWCSPAERRLRVVKRRRRQLFLAVGVLMVLRSLVCEAQMLIILLIIPKDSKEILGYILGLLASVIAWTSRFPALCRANLTQTFIVSGGLCTVSGALYTAAILLHDVRFRFLIRVMPWLLSSIGCVILDLLVSFCTSTWIPFVICTFTEITIIIAPCCMICMFLLSLKEVTLSKDGLDVRPPHRMVRVVRVDGLCSSDTSYSSSSDSTDLEVGGNLDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8V5</accession>
    <name>A0A3B5L8V5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DEAH (Asp-Glu-Ala-His) box polypeptide 32a</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8V5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007247"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007340.1">
        <property type="protein sequence ID" value="ENSXCOP00000007247.1"/>
        <property type="gene ID" value="ENSXCOG00000005601.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157227"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000010">
        <property type="entry name" value="ATP-dependent RNA helicase DQX1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF88">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE DHX32-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="63"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="735" mass="82424" checksum="7A934F8F47F3FE02" modified="2018-12-05" version="1">MSIESISIEADIFSESWEESSSFGFGDDLELNQFDGLPFSSRYYELLEKRKALPVWKVRSKFEDALENNQLVIVSGVAQTGSSTQIPQWCAEFCLSAQFQHGVVVCTQTNKRQAVDLALHVADEMDVNIGHEVGYTIPLETRSSSDTVLRYCTDDMLLREMMSDPFLEQYGVIIIDQAHERTVSTDILLGLLKNVLLQRPELKVVVLTMSAVVDRLLGHYGSVPLIKLEISGSPEVVYTKMNNKDYFYLALALLQEIHLTKEIGNVIVFLASEEDVCRAWSIIQSESPRLEEGLDQITPIVLCPSQQGSSPLLIEQAGTTNSKRVFLLAPKQEAVWLAGQSVNFVIDTGVQKKMVKIYNPRARAFSEVLQPISQCQADVRKHLCSSAGKCFRLYPEASLPTAEIYPQILDCNITPTVLYLKRMEIAGLGQCHFMERPDPEGLMQALEELDYLAALDDDGNLSEIGIIMSEIPLDPQMAKALLASCEFDCVSEMLTIAAMLSAPSCFLQPAGSMTQEAFKCQRKFQHPEGDHFTLINIYNAFKQKQKDQYLNVKTWCQDYFLDHSALNIADTIRSELTSTLNRIELPISEPAFGTKTNTKDIKRALLAGFFMQIARDVDGSGNYLILTNNHVAQLHPFSGYGVQSHKLGLPEWVVFNEYALAENSLRTVSEISPQVFNEMAPMYFFYNLPPSESKDILQDMLDSEESRRCTKKENRNSDTDISCAAGTQTDKCLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8V6</accession>
    <name>A0A3B5L8V6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendritic spine</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8V6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006196"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006267.1">
        <property type="protein sequence ID" value="ENSXCOP00000006196.1"/>
        <property type="gene ID" value="ENSXCOG00000004782.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153491"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08686">
        <property type="entry name" value="C2_ABR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04387">
        <property type="entry name" value="RhoGAP_Bcr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000057">
        <property type="entry name" value="active breakpoint cluster region-related protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000004">
        <property type="entry name" value="active breakpoint cluster region-related protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037769">
        <property type="entry name" value="Abr/Bcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23182:SF3">
        <property type="entry name" value="BREAKPOINT CLUSTER REGION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23182">
        <property type="entry name" value="BREAKPOINT CLUSTER REGION PROTEIN BCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19057">
        <property type="entry name" value="PH_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="DH" evidence="9">
        <location>
            <begin position="31"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="216"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="398"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="10">
        <location>
            <begin position="561"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="778" mass="88744" checksum="0B9C60889D936ACE" modified="2018-12-05" version="1">NIAPVSFFNLCIRTCLYSSQTECDLEKGLEMRKWVLSGILATEEAYLSHLEALLLPMKPLKAAATTSQPVLTVAQIETIFFKVPELYEIHKEFYDGLLPRVQQWSHHQRVGDLFQKLASQLGVYRAFLDNYELAVETAEKCCQANSQFAEISEPVDRVTRSTLVLHDLLKHTPTSHPDHPLLQDALRISQNFLSSINEETTSRRQSLAGKKGENRQLLKDSFMVELVEGTRKLRHVFLFTDLLLCAKLKKQIGGKNQQYDCKWYIPLTELTFQGPEDTEPLTIPQVPDEELDAVKVKISHLRSEIQREKRANKGSKVIDRLRKKLSEQESLLLLTSPSMPLRLFNKNGKSYSFLISSDYERSEWREIIREQQQKCVKTPSLTSMELQMLTSSCLKLQTVHNIPLSLNKEEDESSGLYGFLNVIVHSASGLKQSLNLYCTLEVDSFGFFLNKAKTRVYRYTTEPKWNEEFEIELEGSQTLRLLCYEKSYNRTKLNKEDGDSTDRIIGKGQILLEAQTLQGKDWQRTVIPMNGVEVKLSMKFTSREFSLKRMPSEKPMGVFGVKISTVTKRERSKVPYIVRQCLEEIERRGMEEVGIYRVSGVATDIQALKTAFDTNNKDVSVMMSEMDVNAIAGTLKLYFRELPEPLFTDELYPNFAGGITLTDSVAKESCMLNLLLSLPEPNLVTFLFLLDHLKRVAEKESVNKMSLHNLATVFGPTLLRPAEKDSKIPSNPTQPISMGDSWSLEVMAQVQVLLYFLQLETIPTPDSKRQSILFSTEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8Y0</accession>
    <name>A0A3B5L8Y0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">alanine transaminase</fullName>
            <ecNumber evidence="8">2.6.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Glutamate pyruvate transaminase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Glutamic--alanine transaminase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Glutamic--pyruvic transaminase 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-alanine + 2-oxoglutarate = pyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:19453"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.2" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8Y0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008748"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008854.1">
        <property type="protein sequence ID" value="ENSXCOP00000008748.1"/>
        <property type="gene ID" value="ENSXCOG00000006677.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155265"/>
    <dbReference type="UniPathway" id="UPA00528">
        <property type="reaction ID" value="UER00586"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004021">
        <property type="term" value="F:L-alanine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042853">
        <property type="term" value="P:L-alanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1970:FF:000001">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000226">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000345">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000198">
        <property type="entry name" value="Myosin binding subunit, isoform P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045088">
        <property type="entry name" value="ALAT1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751">
        <property type="entry name" value="ALANINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751:SF480">
        <property type="entry name" value="ALANINE TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="14" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="99"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="132"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="227"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="260"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="16">
        <location>
            <begin position="603"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="320"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="391"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="332"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1011" mass="111248" checksum="89437721F09B1ECA" modified="2018-12-05" version="1">MAADHGELLAEMATVGRLSATERLKHAQKRRAQQLKAWAQMEKDASRGSRAKADKKKKRTTKVKFPNSITLLEAAARNDVEEVRELLNSGVSPDLVNEDGLTALHQCCIDDFVEIVQCLLDAGASVNACDSELWTPLHAAATCGHTGLVQLLVQAGANLLAVNADGNMAYDLCEDEATLELLEMVMAEQGITQKRIDECRGAKELAMLADVEALINTGADLSAQDNNGATLLHIASANGYMSVAELLVDQRAQLELKDSDGWTPLHAAACWGQVLVELLVAHGANLNSKSVLDETPLDVCIDEEVRAKLTDLKNKHDAIMKSQDKQKGTLQRRVSSTGSRGKVVRRVSVNERSSLYRREHHREAIVWQEPATTPEPQDDDEDRQTDIELVGNGETSVSLASSVPGELWSGGGRMDRSGTYELSPAPGQAEGEGADSMTREKSHHTLADLKRQRAAAKLNKYPAPPPPLPSTAEVFFTPASGDPPLLKLRAPEEEQTNNKEPCCVPQISSGYLEFLRAEHKAARMSQQAVNGVSCRGKVLTVENMNPNVKRVEYAVRGPIVQRAVQIEKELREGVKKPFTEVIKANIGDAQAMGQRPITFFRQVLALCSYPELLEDNKFPEDAKKRARRILDACGGHSIGAYSASQGIECIRQDVARYIERRDGGIPSNPDNIYLSTGASDAIVTVLKLLVCGEGRDRTGVMISIPQYPLYSAALADLAAVQINYYLDEDNCWSLSVAELKRALNEAKQHCKPRVLCIINPGNPTGQVQSRQCIEDVIQFAKEEHLFLMADEVYQDNVYAEGCKFHSFKKVLFEMGPEYSSTVEMASFHSTSKCYMGECGFRGGYMEVINMDPEVKAQLTKLVSVRLCPPVPGQALLDLVANPPQPDEPSYTLFTKRTAVLAALAEKARLTEEIFNKVPGITCNPVQGAMYTFPRITLPQKAIDKAEGKVPDMFYCMKLLEEEGICLVPGSGFGQKDGTFHFRMTILPPTEKLKVVLQKIRDFHLRFTNEFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L969</accession>
    <name>A0A3B5L969_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Glucocorticoid modulatory element binding protein 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L969"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004354"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004407.1">
        <property type="protein sequence ID" value="ENSXCOP00000004354.1"/>
        <property type="gene ID" value="ENSXCOG00000003436.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00410000025596"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.390.10:FF:000003">
        <property type="entry name" value="glucocorticoid modulatory element-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.390.10">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059099">
        <property type="entry name" value="GMEB1/2/Spe-44_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010919">
        <property type="entry name" value="SAND-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000770">
        <property type="entry name" value="SAND_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10417">
        <property type="entry name" value="GLUCOCORTICOID MODULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10417:SF3">
        <property type="entry name" value="GLUCOCORTICOID MODULATORY ELEMENT-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01342">
        <property type="entry name" value="SAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25892">
        <property type="entry name" value="Spe-44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00258">
        <property type="entry name" value="SAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63763">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50864">
        <property type="entry name" value="SAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SAND" evidence="11">
        <location>
            <begin position="71"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="349"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="360"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="598" mass="64422" checksum="128ED266640772A3" modified="2018-12-05" version="1">QGDVILRGSGELKCRVTRGRTCSVVRAMANTNEVTVSVGDMVMVKNEDEGDSDDPNKTQVILQLQPISTGDESAETDAAVMTEGDDVEIGCPITCGDCKAVLLVKKFVCPGINVKCVKYEDQLISPKQFVHLSGKATLKDWKRAIRMGGVMLRKMMDSGQLDFYQHSTLCTNTCRSTKFDLLINNTRFPPDSTGLTTPTSSQVQTVLGNGGTVTDDRAEIMGRKLDWSSSSMESGDKKGSSEISEDTLNFWKGIADVGLLGEVVTNINTELIQMLDGVLQRREQNTLQDTEVAVLSNLAQVFGLLDSVKKILERRKQQADPSQEQILSTLSNLEVQLEEQKKQQQVRALLSSPQAAKSKAIAKRPTKRPRLHRPASTTTLLTSPISQQATLQPQQFTVLSPISLSSVGQPFTVAGLPIASLAQSSNTVTLLPAGSQLFTRYMVTGDGKGDAITLHPSSGLTLVGTTAVQDSSQLGTVMSPVELVHLSQHAGGAEMVPIEGQVVDGTMLVQQEVMQGDMEAGQEHTVIEINPTPVEQTVGMMELQLAEEPGREATAMVVQSGIEVTMEADGLQLDASGQLSGVQIVVIEENTQDDNKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9B8</accession>
    <name>A0A3B5L9B8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">High mobility group box 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HMGB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9B8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007070"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007158.1">
        <property type="protein sequence ID" value="ENSXCOP00000007070.1"/>
        <property type="gene ID" value="ENSXCOG00000005463.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183120"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21978">
        <property type="entry name" value="HMG-box_HMGB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21979">
        <property type="entry name" value="HMG-box_HMGB_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000006">
        <property type="entry name" value="High mobility group protein B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000015">
        <property type="entry name" value="high mobility group protein B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017967">
        <property type="entry name" value="HMG_boxA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF28">
        <property type="entry name" value="HIGH MOBILITY GROUP BOX 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09011">
        <property type="entry name" value="HMG_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00886">
        <property type="entry name" value="HIGHMOBLTY12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00353">
        <property type="entry name" value="HMG_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HMG box" evidence="10">
        <location>
            <begin position="10"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="10">
        <location>
            <begin position="96"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="8">
        <location>
            <begin position="10"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="8">
        <location>
            <begin position="96"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="163"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="201" mass="23097" checksum="A862DEA64A2CAE1F" modified="2018-12-05" version="1">MAKGTDPKKPRGKMSSYAYFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSPKEKGKFEDMAKQDKLRYEREMKNYVPPKGQKKKRFKDPNAPKRPPSAFFLFCADFRPKVKGESPGLSIGDTAKKLGEMWNSLSGEEKQPYEKKAAKLKEKYDKDIVAYRTKGSVDSAPAAAANDDEDDDEEEGDEEEEEEEEDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAI9</accession>
    <name>A0A3B5LAI9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Junctophilin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the junctophilin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAI9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009213"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009324.1">
        <property type="protein sequence ID" value="ENSXCOP00000009213.1"/>
        <property type="gene ID" value="ENSXCOG00000007026.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156130"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030314">
        <property type="term" value="C:junctional membrane complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016529">
        <property type="term" value="C:sarcoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000001">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000003">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000012">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017191">
        <property type="entry name" value="Junctophilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23085">
        <property type="entry name" value="GH28348P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23085:SF6">
        <property type="entry name" value="JUNCTOPHILIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037387">
        <property type="entry name" value="Junctophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="621"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="228"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="420"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="230"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="430"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="476"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="520"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="590"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="642" mass="70486" checksum="8CED5A3FC540A491" modified="2018-12-05" version="1">MTGGRFDFDDGGTYCGGWEDGKAHGHGICTGPKGQGEYAGSWSHGFEIVGVYTWPSGNTYKGYWSQGKRHGLGVENKGRWIYRGEWSHGFKGRYGVRQSHNTPARYEGTWSNGLQDGYGVETYGDGGTYQGQWMGGMRHGYGVRQSVPYGMATVIRSPLRTSMASLRSEQSNGAVLHDLSSPVDTPTGTRGGFVLNFHSDSEVVTGKKKGLFRRGSLFGSLRQLRKSDSRTSISSKRSSARSDATMSRISSSDANSTISIGDGELPDEDLPLEDHVDATTTETYMGEWKNDKRNGFGVSERSNGMKYEGEWLNNKRHGYGCTVFPDSTKEEGKYKNNVLVRGIRKQLIPLKNPKTKEKVDRAVEGAQRAAAIARSKVEIAASRTAHARTKSEAAEQASISAVHESEIARAVARELSPNFYQPGPDYIKQQSKEPVEIKEVPVEKKEESPHDSPHFYRKGTTPPHSPVASPKSSYKASKDERSSRKLSKEERLSFTDGPKTSHTHIEAPPKPTKVQQPAPATGPPVQPPTVPVNGQLHSEYHSYYVKATPRGPPPPEPEEVEEEPSAQALARLPPQPKSFSTPIPTPKPPSIRDSKSDPKLRKQDSLKPKSLADTKKGPNSILVAMVMLLNIGLAIIFVHFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LB43</accession>
    <name>A0A3B5LB43_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GADD45 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027884635.1">
        <property type="nucleotide sequence ID" value="XM_028028834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LB43"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009418"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009529.1">
        <property type="protein sequence ID" value="ENSXCOP00000009418.1"/>
        <property type="gene ID" value="ENSXCOG00000007175.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114151556"/>
    <dbReference type="GeneTree" id="ENSGT00950000182964"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000012">
        <property type="entry name" value="growth arrest and DNA damage-inducible protein GADD45 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024824">
        <property type="entry name" value="GADD45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10411">
        <property type="entry name" value="GROWTH ARREST AND DNA DAMAGE-INDUCIBLE PROTEIN GADD45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10411:SF5">
        <property type="entry name" value="GROWTH ARREST AND DNA DAMAGE-INDUCIBLE PROTEIN GADD45 BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="2">
        <location>
            <begin position="22"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="160" mass="17707" checksum="B4C258B5CD8623CF" modified="2018-12-05" version="1">MTLEEVVGTNSTEQKMETVSQAVEELLVAAQRQDCLTVGVYESAKLMNVDPDSVVLCLLATDEEDEDDIALQIHFTLLQAFCCDNDINIVRVSGVRRLSQLLGEDTGDSNGNEPRDLHCILVTNPPVQPLQCQALQHVSSFCEQSRCSNQWVPCLELRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCM6</accession>
    <name>A0A3B5LCM6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zgc:195245</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCM6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008389.1">
        <property type="protein sequence ID" value="ENSXCOP00000008290.1"/>
        <property type="gene ID" value="ENSXCOG00000006350.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011228"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009533">
        <property type="entry name" value="FAM107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16768">
        <property type="entry name" value="DOWN REGULATED IN RENAL CARCINOMA 1/TU3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16768:SF7">
        <property type="entry name" value="PROTEIN FAM107B-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06625">
        <property type="entry name" value="DUF1151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="87"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="146" mass="17358" checksum="8F61115929D2B192" modified="2018-12-05" version="1">QFSQKPGYGNHKREMPVGPLSLAHNSAVDGSGSEIIQLRKINGSSAETPSYQHLHRELLLSHKRGLLLEEKPELKRVLEQRRLELHREEEMAQRRPSDLETELRKRQQKLQEYEQEEIRQRENQNKIPEFVRVRENLRRTQTFDQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LD25</accession>
    <name>A0A3B5LD25_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LD25"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008853.1">
        <property type="protein sequence ID" value="ENSXCOP00000008747.1"/>
        <property type="gene ID" value="ENSXCOG00000006679.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01070000253760"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004020">
        <property type="entry name" value="DAPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02758">
        <property type="entry name" value="PYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01289">
        <property type="entry name" value="PYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50824">
        <property type="entry name" value="DAPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Pyrin" evidence="3">
        <location>
            <begin position="38"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="4">
        <location>
            <begin position="208"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="653" mass="76116" checksum="D16B9F2F2936077F" modified="2018-12-05" version="1">MKPVCWTLTRKKNKEEVTEPSNQLELIQEPEEIKHPSLTLQFTFKALCETLKKLQENDFRMFKWKLWSHYPQPFSASQSMDIVDLVDRLLESFTCEMSVQITRTILVSVSKKNVAEHLRMLYIKNQIRHELCELMIQKYSEVSITEGQTMPLDDIYVDVSTLSCFDNGPNIEHEVMQIAKPYTKKNPGKRQSLAETVSQEWVTKSHARQVYLYGLAGSGKSMFVKKLALDWARGRSHQNFSFVYVIAIREMNEYLETKVSLMDIVNKLYPMAQRIKTEGLRYENNEALFIFDGLDEYPEEFNFNRTEIHSDATCPMTMRVLLTNLLRGRFLSRSRFMITSRPHADRCAPWDGHYDEIEMGSFSDENKDEYFRKRFKDPTQATRVIEHVKSVKTLHIMCCLPLFCTLLADECQLVFRESRTEEKLPTNITYMYTKLLLKLVHIRRTRAPDFSPEEELKLLMDLGKFAFTLLEKGQFHIKRTKCPEISHIEAVNRSGICTQYMTTPFVLCHEDVVCFLHPTMQEYMAALYAYLSFRNHDKNIFDQPIKSKFRLSTKGHKIMELYKAAVEKSLQCPDGKLDMFLRFLFGMGCKTNQELLLPFHKPPSSHHSTVFMYIKKITSLSNQGISGGGLNANTHFQYLFIFFNTLASQLCCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFE5</accession>
    <name>A0A3B5LFE5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFE5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009562"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009674.1">
        <property type="protein sequence ID" value="ENSXCOP00000009562.1"/>
        <property type="gene ID" value="ENSXCOG00000007283.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00490000044642"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33244">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33244:SF3">
        <property type="entry name" value="PEPTIDASE A2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="223" mass="24757" checksum="DC23AE77B9B1A103" modified="2018-12-05" version="1">MDQHKVVYGPQEERQGYVGPFTLIQSTCVISSRYKSGSSVMKATDPYMALLAYRATPLQNGYSPAQLLMGRRLRTTVPTHPSELQPALPDCSSLFQKEREKRMSDATNFNRRHRAKPLSSLSPGQEVWITDTKTSGTVIQSHTSPRSYLVDAPLGVVRRNRLHLIPLQSPAQDEGGQQQQEPLPVLEQDHAPPALGFPVPSPGVSTPRNRSGRVIKQPTRLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LG28</accession>
    <name>A0A3B5LG28_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytosol aminopeptidase</fullName>
            <ecNumber evidence="8">3.4.13.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cysteinylglycine-S-conjugate dipeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Leucine aminopeptidase 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Proline aminopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Prolyl aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Cytosolic metallopeptidase that catalyzes the removal of unsubstituted N-terminal hydrophobic amino acids from various peptides. The presence of Zn(2+) ions is essential for the peptidase activity, and the association with other cofactors can modulate the substrate spectificity of the enzyme. For instance, in the presence of Mn(2+), it displays a specific Cys-Gly hydrolyzing activity of Cys-Gly-S-conjugates. Involved in the metabolism of glutathione and in the degradation of glutathione S-conjugates, which may play a role in the control of the cell redox status.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-cysteinylglycine + H2O = L-cysteine + glycine</text>
            <dbReference type="Rhea" id="RHEA:28783"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:28784"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>S-benzyl-L-cysteinylglycine + H2O = S-benzyl-L-cysteine + glycine</text>
            <dbReference type="Rhea" id="RHEA:62568"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:145802"/>
            <dbReference type="ChEBI" id="CHEBI:145803"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:62569"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>an S-substituted L-cysteinylglycine + H2O = an S-substituted L-cysteine + glycine</text>
            <dbReference type="Rhea" id="RHEA:60444"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58717"/>
            <dbReference type="ChEBI" id="CHEBI:143103"/>
            <dbReference type="EC" id="3.4.13.23"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:60445"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M17 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.13.23" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LG28"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006819"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006902.1">
        <property type="protein sequence ID" value="ENSXCOP00000006819.1"/>
        <property type="gene ID" value="ENSXCOG00000005275.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063255"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00433">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008283">
        <property type="entry name" value="Peptidase_M17_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963:SF23">
        <property type="entry name" value="CYTOSOL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="LEUCINE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02789">
        <property type="entry name" value="Peptidase_M17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017454138" description="Cytosol aminopeptidase" evidence="16">
        <location>
            <begin position="25"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Cytosol aminopeptidase" evidence="17">
        <location>
            <begin position="347"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="481" mass="51613" checksum="E267B582F5AE292C" modified="2018-12-05" version="1">VICLPFPVDLCVCVCVCLQGLVLGVFEKDGEEESGHLTEAAARFDQSVSGKLSELLKISGPGLKKGKSRLFYGIHQDFPCVAVVGLGKDSEGVCGAEKWDSRKENIRQAVSAGCRTLQELEVGHVEVDDCGDAKSAAEGAVLGLFSYDELKSKKKTRVTSQLHGSADVDGWKKGIIYAEGQNLARFLMEAPANRITPTAFANTMEERLAPHAERVTIKKRSKAWMEEQQMGAFLSVARGSEEPPVFLELHYSGSADSQQPLLLVGKGITFDSGGISLKPSQSMDAMRADMGGAATVCASIVAAAALKLPVNIVGGLLLIPFCQCLHFYCAQTNILHVCARSRSGVDNTDAEGRLILADALCYGHTFNPRAIVNVATLTGAMDVALGSAATGVFTNSDWLWEQLQQVDRNLTFEHDNYEGSSGGACTAAAFLREFVTAPHWAHLDIAGVMSNKDEVPYLRKGMSGRPTRTLVEFAARLAHSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHK1</accession>
    <name>A0A3B5LHK1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gametogenetin-binding protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Protein ZNF403</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in spermatogenesis.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHK1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007334"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007429.1">
        <property type="protein sequence ID" value="ENSXCOP00000007334.1"/>
        <property type="gene ID" value="ENSXCOG00000005671.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009552"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026073">
        <property type="entry name" value="GGNBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13601">
        <property type="entry name" value="GAMETOGENETIN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13601:SF2">
        <property type="entry name" value="GAMETOGENETIN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="456"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="343"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="456"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00031743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="669" mass="75906" checksum="FF0518984412A793" modified="2018-12-05" version="1">MARLVAVCREGEEDYPFLARQIPLYINDTLTMVMEFPDSFMDAEGTDIVIYYSKLKQQDLNIAFMVSSSEVYSALSQLVPCVGCRRSVERLFSHSVETGNPALEPLTVKPKGMLTVTKACLADMKKLYTLFYIHGPKLNDMIDAIPKSKKNKRCQLHSLDTHKPKPLGGSWMDVWELMSQECRDEVVLIDSACLLETLETYLRKHRFCTDCKNKVIRAYNILVGEVDSSEEKGYCAALYEGLCCCPHERHIHVCCETDFIAHLLGRAEPEFSGGYERRERHAKTIDIAQEEVLTCLGIHLYERLHRLWQKLRAEEQTWQILFHLGIDALRKSFEMAVEKMQGISRLEQFVEELSEEERAKELKLEKKRQKRKNRRKNKCGFDMSEQEAENKEKILDEGSFDSVEGSCKACGSHDEEEEEAGCEETVTTTRNASCNCSDNTKRGIIIRDTTLSSDLSHNSNGSDCGYSSSMEGSEMGSREGSDVACSEGMCNHEAGDYPNVHHCVEDKEEDGTDSCVDCWPHSEENSQCKSKKKKKKGCTSDGNATGHNLTSANTCRTKDIFSSLCGDKLANISLRSPWAVHQSNLSLHMRPTKANLSLVELLDDSEVTSDEESGLTEDEIQAFLEQNQSFYSNRHQYRQLLKEKFTNYCRATERSKRVCGKWFATTSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHT5</accession>
    <name>A0A3B5LHT5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHT5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011688"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011821.1">
        <property type="protein sequence ID" value="ENSXCOP00000011688.1"/>
        <property type="gene ID" value="ENSXCOG00000008827.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000243802"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039341">
        <property type="entry name" value="CFAP99"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34649">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34649:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="199"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="268"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="422" mass="49828" checksum="C6497B0934CEC1BD" modified="2018-12-05" version="1">VSCRWRPEINHLMDQLSKKERRARTKLTQCQDFILSKNKLPTPPKEELSPLPDKFTPVRITFILTIFKHFLQQLSLTARVCILIPNRKQIIEELVQGSTDLSPFLQRQKELEERERQEKRQKEERTRLETQITEQEIALNRKRIAEQKHEAVQRKKEETARIMQRYAKKRLKEEKEVRHLVQQVMEDRINLKVAQERSKKIKQKIVKEVSEENQELLRQALEEQEEELRKRFQIVHEIRAIKSLHIVKPNNFNDTETAGYELLGEMSLAELKERLALLRQNQQKEEEERRSNILQEKLKKQQQILETLDNIDLYRQLKEERRAQQELLKQVVAEDSTIVALKKKLEDKKQELHKVKETERSRAKLRQRFASRASIQVSFLQGSISLPASTSVVLLSFLPPERVKGDNMGGTGAELSPLRSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIF4</accession>
    <name>A0A3B5LIF4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucuronosyltransferase</fullName>
            <ecNumber evidence="3">2.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.17" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIF4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010543.1">
        <property type="protein sequence ID" value="ENSXCOP00000010420.1"/>
        <property type="gene ID" value="ENSXCOG00000007893.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159677"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000066">
        <property type="entry name" value="UDP-glucuronosyltransferase 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF161">
        <property type="entry name" value="UDP GLUCURONOSYLTRANSFERASE FAMILY 1 MEMBER A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="309" mass="35007" checksum="C6D5568218B1BD87" modified="2018-12-05" version="1">MAVEGRGAAGKVKDSAETPKLVKTSKVKETDTILEKSVSSSGFLGNLLVVPMDGSHWVGIKAIAQEMGRRGHRVIVVIPELSIRMGPGKYYDTITYPVPYGRDYMDFVMASHKDILEKSAQPLMEKIKKRFGQIQKIMSFIHTTAESLLFNSSLISHLAQQNFDAVLTDPMVPTGLLIAHKLGIPTINLLRGVPCSLDMKASGCPSPPSFVPRFFTGFTDRMSFKERLINTLVALLEPLFCRLMYWHFDQIAYDFLEEKVSVAELFDVIRLFFYLIFSSTLFSSYKNYKNKSKIKLKLYKKYIDFAFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIL4</accession>
    <name>A0A3B5LIL4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Myosin tail domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIL4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011928"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012066.1">
        <property type="protein sequence ID" value="ENSXCOP00000011928.1"/>
        <property type="gene ID" value="ENSXCOG00000009033.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164626"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.370:FF:000002">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014751">
        <property type="entry name" value="XRCC4-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349">
        <property type="entry name" value="CINGULIN-LIKE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349:SF6">
        <property type="entry name" value="MYOSIN-6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0505">Motor protein</keyword>
    <keyword evidence="9" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="7" id="KW-0518">Myosin</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin tail" evidence="12">
        <location>
            <begin position="3"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="202"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="256" mass="29561" checksum="1B5CB118D9694494" modified="2018-12-05" version="1">MVAEIEELRAALEQTERARKVAEQELVDASERVGLLHSQNTSLLNTKKKLESDLVQVQGEVDDAVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEVTVKDLQHRLDEAENLAMKGGKKQLQKLEQRVRELETEVEGEQRRGADAVKGVRKYERRVKELTYQTEEDKKNLARLQDLVDKLQLKVKAYKRQAEEAEEQANSHMSRLRKVQHEMEEAQERADIAESQVNKLRAKSRDLYNFVFGQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LME4</accession>
    <name>A0A3B5LME4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LME4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012021.1">
        <property type="protein sequence ID" value="ENSXCOP00000011885.1"/>
        <property type="gene ID" value="ENSXCOG00000008998.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159013"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="150" mass="16957" checksum="02ADBBCC3483F5C6" modified="2018-12-05" version="1">MSERRRSAAALSSRAHAFSVEALIGSNKKRKLRGWEEKELELSMESLATDGEDPAHCLDMDPGQCAHTHTHTHICIYIYIYIAYIISLKKTNTAKSSRQVSLRRGSTPMDPRLPTQSAYAPQSNPEKCIRSSAAAFQQKHRRETQLLQFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNL8</accession>
    <name>A0A3B5LNL8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transmembrane channel-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the TMC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNL8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012325"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012474.1">
        <property type="protein sequence ID" value="ENSXCOP00000012325.1"/>
        <property type="gene ID" value="ENSXCOG00000009318.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244942"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038900">
        <property type="entry name" value="TMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012496">
        <property type="entry name" value="TMC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302:SF35">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23302">
        <property type="entry name" value="TRANSMEMBRANE CHANNEL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07810">
        <property type="entry name" value="TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="297"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="452"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="510"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="560"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="617"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="TMC" evidence="8">
        <location>
            <begin position="440"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="692"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="751"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="880"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="945"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="704"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="757"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="987"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1027"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1051"/>
            <end position="1066"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU310713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1090" mass="124030" checksum="86FDF925FF09CBB7" modified="2018-12-05" version="1">CLKRQLNKRHLSIYVPAPSSIYDKEVDSNDPEEMFQNIQYQKEIIANIRSRPWPMRRKLKVLKQAREIVLKYEGQLTRTRGYQTAGADLLKKLYRVLYNIVVTFIPWEVRIKKIESHFGSGVASYFIFLRWLFGINIVLTIMTGSFVVLPELLAGAPFGTTRSKTIPKEHLASAQDLDTIWSLGGYLQYSVLFYGYYGSVRKIGSAGYRLPLAYFLVGMAVFAYSFIILLRKMAKNSRLSLASASDENFTFCWRVFCAWDYLIGNPEAAECKGAAIVNNIRVSYTLTQISFLISLRILANILVLLSLAGSIYIIYFVVDRSQKLEQEKPELTLWEKNEVSVVVSLITMIAPSAFELVAQLEMYHPRTSLRFQLARVLVLYLGNLYSLIIALLDKVNSMSSAVSIEILCTILLGNFSTICQEHTFSTIPFEKNARSQQDQCWETYVGQEMLKLSIIDMIFTVASILLIDFIRGLVVRYLSDCCCWDLESKFPEYGEFKIAENVLHLIYNQGMIWMGAFFSPCLPAFNVLKLIGLMYLRSWAVLTCNVPHQQVFRASRSNNFYLAMLLFMLFLCMLPTIFAIVRYRPSEHCGPFSGQEKIYDIISDTVASDFPLWFSKVMSYVTSPVVVLPAVLLLFMLIYYLQAIARSLKFTNNQLRIQLQTVSFKIVTMTVCKKCCDCVSTATITARLQVPEASADKNPDQQESDIPNQEASIHTSSPQQNGSITNVQSPINRSNSIRTISQSASGADLNEGCVSGSVRTPQPSAQSVSPDITPIHPGSCRSKSCHHMAYNPPSDLSNAIHTDPLYRKTIRSLHPVEAAGIIAAQNYGGHCGPTTRYVIVNEHRPRKKLLCSTTRIPRHYLMEEPREILELYPRNIKHYTPRTIHQQHPPRSHTSQQLRKEEEETGKDRKRTSLGKSLRPCSLPDHHQQAHFYIGERLDSFRESQRARYEMQEDDEEEAGKEGIDWGDFNLPSPSQTSVKEKESSHTQRQPVHSPRRQYQSPTRTRHMGSQLKPKLRGQLETHLTESDSASIASSSDQQNSSTDQYIHVIHSKERHLGSDVRQEKMSKKKSKTNFDLNYTESNDLICSNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LP27</accession>
    <name>A0A3B5LP27_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cystatin-B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Stefin-B</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cystatin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LP27"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012485"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012636.1">
        <property type="protein sequence ID" value="ENSXCOP00000012485.1"/>
        <property type="gene ID" value="ENSXCOG00000009434.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154826"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071220">
        <property type="term" value="P:cellular response to bacterial lipoprotein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.10:FF:000001">
        <property type="entry name" value="Cystatin-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018073">
        <property type="entry name" value="Prot_inh_cystat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001713">
        <property type="entry name" value="Prot_inh_stefin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11414:SF21">
        <property type="entry name" value="CYSTATIN 14A, TANDEM DUPLICATE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11414">
        <property type="entry name" value="CYSTATIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00295">
        <property type="entry name" value="STEFINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00287">
        <property type="entry name" value="CYSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0789">Thiol protease inhibitor</keyword>
    <feature type="domain" description="Cystatin" evidence="9">
        <location>
            <begin position="2"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="100" mass="11121" checksum="ED150FC415319B6D" modified="2018-12-05" version="1">NMLCGGTTAAAQDATAEIQEICNTMKTKVEQKAGKTYETFTAKSYKSQVVAGTNFFIKVHVGGDEHIHIRVFKKLPCHGQDCELTNIQLSKSHQDPIEYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPB4</accession>
    <name>A0A3B5LPB4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyroglutamyl-peptidase I</fullName>
            <ecNumber evidence="5">3.4.19.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro.</text>
            <dbReference type="EC" id="3.4.19.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C15 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.3" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPB4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013933"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014105.1">
        <property type="protein sequence ID" value="ENSXCOP00000013933.1"/>
        <property type="gene ID" value="ENSXCOG00000010552.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015368"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016920">
        <property type="term" value="F:pyroglutamyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.20">
        <property type="entry name" value="Peptidase C15, pyroglutamyl peptidase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016125">
        <property type="entry name" value="Peptidase_C15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036440">
        <property type="entry name" value="Peptidase_C15-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033694">
        <property type="entry name" value="PGPEP1_Cys_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402">
        <property type="entry name" value="PROTEASE FAMILY C15 PYROGLUTAMYL-PEPTIDASE I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402:SF1">
        <property type="entry name" value="PYROGLUTAMYL-PEPTIDASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53182">
        <property type="entry name" value="Pyrrolidone carboxyl peptidase (pyroglutamate aminopeptidase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01334">
        <property type="entry name" value="PYRASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="active site" evidence="5">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="140" mass="15323" checksum="96A7A6F71A62DB21" modified="2018-12-05" version="1">MDSVSGIATSVTLEQCGHNMGYTRLDNRSFCPASHCCMENGPDYIKSVIDMDTVCKRVNGSNVGITVSVSKDAGRYLCDYTYYTSLHQGQGCSAFVHVPPLGGPYSSEELGRGLQAVVKEMLNLLEVDYNEWGKRQTLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPE4</accession>
    <name>A0A3B5LPE4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">N-terminal Xaa-Pro-Lys N-methyltransferase</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. NTM1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPE4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009714"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009828.1">
        <property type="protein sequence ID" value="ENSXCOP00000009714.1"/>
        <property type="gene ID" value="ENSXCOG00000007388.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008371"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071885">
        <property type="term" value="F:N-terminal protein N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008576">
        <property type="entry name" value="MeTrfase_NTM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753">
        <property type="entry name" value="AD-003 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753:SF2">
        <property type="entry name" value="N-TERMINAL XAA-PRO-LYS N-METHYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05891">
        <property type="entry name" value="Methyltransf_PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="136" mass="15887" checksum="EB5F7553323A1342" modified="2018-12-05" version="1">MADMMEHFLLHAHEEYLGDAADRIETYYCFNLQEFTPPRNKYDVIWMQWVACHLTDKDLLNFLIRCKKSLRPNGVLIIKDNMARQGCKLDPIDSSISRHLDIMKVIVSKAGLEILAVERQEGFPEVIMPVWMMAMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPR1</accession>
    <name>A0A3B5LPR1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPR1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011426"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011558.1">
        <property type="protein sequence ID" value="ENSXCOP00000011426.1"/>
        <property type="gene ID" value="ENSXCOG00000008628.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165492"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05382">
        <property type="entry name" value="CAP_GAPR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000002">
        <property type="entry name" value="Golgi-associated plant pathogenesis-related protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034113">
        <property type="entry name" value="SCP_GAPR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP" evidence="1">
        <location>
            <begin position="6"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="150" mass="16876" checksum="AD8C7F14430D5C52" modified="2018-12-05" version="1">MTGNETFKKQFLDAHNNYRAAHGAQNLTYNGDLNSAAQKWAEHLLQSNTMKHSNIEDGENIYYSYNSEKVTPQGKSAVDSWYGEIKDYDFTRPGVQPKTGHFTQVVWKDTKELGVGVATDGHTVFVVGQYRPGGNITNAGYFEKNVQQKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPZ1</accession>
    <name>A0A3B5LPZ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">MPN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Probable protease. Acts as a sensor of N(6)-methyladenosine methylation on DNA (m6A): recognizes and binds m6A DNA, leading to its degradation. Binds only double strand DNA (dsDNA) in a sequence-independent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M67 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPZ1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013082"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013242.1">
        <property type="protein sequence ID" value="ENSXCOP00000013082.1"/>
        <property type="gene ID" value="ENSXCOG00000009913.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160191"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08067">
        <property type="entry name" value="MPN_2A_DUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000053">
        <property type="entry name" value="MPN domain-containing protein CG4751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040843">
        <property type="entry name" value="RAMA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18755">
        <property type="entry name" value="RAMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="10">
        <location>
            <begin position="233"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="152"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="432" mass="48102" checksum="D734E8B1878BE048" modified="2018-12-05" version="1">VELFLSVSSGSEPPGSPQVVEDGGEEDEEELSGGEEAEFRSSSGRGSLLTRRGITLRVLLKDGLVEPGDGVLAIHYLGKNFVGDLLNDGKIRWVETGQIFNSPSAWATHCKRLVNPAKKSGCGWASVRYRGQKLAQYKTTWLHKYQPSADMSMVSEEDDDEDEEEGKAAVQADEKNKNNKPGLHDVMVSRRADRERIPVRYCSLGTRDAARDPHTLVELSAFSAINRFQPFNVAVSSNVLLLMDFHCHLTTSEVVGYLGGRWDTNTQLLTVLRAFPCRTRLADRDTASAVEEEICQNLFMRGLSLVGWYHSHPRGPALPSLQDIDSQMDHQLRLQGSNNGFQPCLGIICGPYYHGNQGVASTITPFWVVPPPEMLLVDYYRTAPDLVQFNQYWCPETTMLDKVKGSLSCHAPKDQAYSQILEHVYSQLSTTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQ31</accession>
    <name>A0A3B5LQ31_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Neuroendocrine convertase 1</fullName>
            <ecNumber evidence="6">3.4.21.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Prohormone convertase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Proprotein convertase 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of protein hormones, neuropeptides and renin from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.</text>
            <dbReference type="EC" id="3.4.21.93"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
            <location evidence="4">Secretory vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase S8 family. Furin subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.93" evidence="6"/>
    <dbReference type="RefSeq" id="XP_027857987.1">
        <property type="nucleotide sequence ID" value="XM_028002186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQ31"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012885"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013042.1">
        <property type="protein sequence ID" value="ENSXCOP00000012885.1"/>
        <property type="gene ID" value="ENSXCOG00000009745.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114135174"/>
    <dbReference type="CTD" id="5122"/>
    <dbReference type="GeneTree" id="ENSGT00940000157385"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034774">
        <property type="term" value="C:secretory granule lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016486">
        <property type="term" value="P:peptide hormone processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000054">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.850:FF:000001">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000010">
        <property type="entry name" value="Putative neuroendocrine convertase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.850">
        <property type="entry name" value="Peptidase S8, pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022005">
        <property type="entry name" value="Proho_convert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032815">
        <property type="entry name" value="S8_pro-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038466">
        <property type="entry name" value="S8_pro-domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF14">
        <property type="entry name" value="NEUROENDOCRINE CONVERTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12177">
        <property type="entry name" value="Proho_convert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16470">
        <property type="entry name" value="S8_pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="17" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 21 22" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 21 22" id="KW-0645">Protease</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 21 22" id="KW-0720">Serine protease</keyword>
    <keyword evidence="10 24" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017328298" description="Neuroendocrine convertase 1" evidence="24">
        <location>
            <begin position="27"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="25">
        <location>
            <begin position="467"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="633"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="684"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="684"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="20 21">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="20 21">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="20 21">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00032862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="772" mass="86139" checksum="4AA99A0EF71966E3" modified="2018-12-05" version="1">MEVRCRPAVMCCVFAVLCSALSRSQALSYADRQYLNEWAVEIPGGLSEAEAIAKELDYQLVRQIGALENLFLFKHGSHPRRMRRGAEHITRQLSEDDRVLWAEQQYEKRRSKRAALRECRDCSVDKLFDDPMWNQQWYLQDTRSSSSLPKLDLHVIPVWQKGITGKGVVITVLDDGLEWNHTDIYANYDPAASYDFNDNDPDPFPRYDSTNENKHGTRCTGEIAMQADNNKCGVGVAYNSKVGGIRMLDGIVTDAIEASSIGFNPNHVDIYSASWGPNDDGKTVEGPGRLAQKAFEYGIQQGRNGKGSIFVWASGNGGRQGDNCDCDGYTDSIYTISISSASQQGLSPWYAEKCSSTLATAYSSGDYTDQRITSSDLHDDCTQTHTGTSASAPLAAGIFALALEQNPDLTWRDLQHIVVWTSEFDPLANNPGWKRNGAGLMVNSRFGFGLLNAKALVDLADPAVWKHMPEKKQCIVRDDAFQPRELKAAGEITIEIPTKACEGQENAIRSLEHVQVEASIEYTRRGDLHITLTSPAGTTTVLLAERERDTSSNGFKNWDFMSVHTWGEDPAGTWTLKITDTSGRMENKGRILNWKLILHGTSEKPEHMKKPRVYIPYNAVQNDRRGVEHMDDMMEEPTQPRPPQKTGAAEASPSVSEKEPKSPSSGSPRRPSLALLRLLQMAFKQQATSSQRPGATARPLSARRKQQSRGFLPLPTKLPAHKLYQALDLINKFSGPGDSLYSDYSDGFYNIKPYKHRNDRLLQALFEMLDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQJ4</accession>
    <name>A0A3B5LQJ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Actin-related protein 2/3 complex subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the WD repeat ARPC1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQJ4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014383"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014567.1">
        <property type="protein sequence ID" value="ENSXCOP00000014383.1"/>
        <property type="gene ID" value="ENSXCOG00000010912.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183183"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000030">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017383">
        <property type="entry name" value="ARPC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10709">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2/3 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10709:SF17">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038093">
        <property type="entry name" value="ARP2/3_su1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="48"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="384" mass="42694" checksum="885554514EB407DF" modified="2018-12-05" version="1">MSLHSFGLEPLSCHAWNKDRTQIAVSPNNNVVNIYEKKGKDWHKIHELTEHSGRITGVDWAPVSNRIVTCASDRNAYVWTLKDGVWKPTLVLVRINRAATCVKWSPLENKFALGSGARLISVCYFEQENDWWLSKHIKKSIRSTVLSLDWHPNNILLAAGSADLHCRIFSTYIKEIEDKPGPTAWGTKMPFGELLLEHKDCGGWVHSVSFSPGGDQLAWVAHNSSLTVVDAAQGKEVTQLTTDLLPLLSVLYVNPTDIVAAGHDCCPYLFTYTGPHALECVKKLDIPKQTSKGSLSAMQHFRNLDKKATEEDNNELETLHQNSIMQLCVVSGEKAKVEKYSSVGLDGAMVIWDFKVQCQVKAVSSNGQPLVLSAIPALWLLPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LR43</accession>
    <name>A0A3B5LR43_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Coiled-coil domain containing 85C</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CCDC85 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LR43"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011876"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012012.1">
        <property type="protein sequence ID" value="ENSXCOP00000011876.1"/>
        <property type="gene ID" value="ENSXCOG00000008988.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159071"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019359">
        <property type="entry name" value="CCDC85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13546:SF14">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 85C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13546">
        <property type="entry name" value="RE60986P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10226">
        <property type="entry name" value="CCDC85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="19932" checksum="255F37828EDB23DF" modified="2018-12-05" version="1">MEDLIRLSDQDLQRFGKEDLIRTLRALQNRSTDVMLEHGKLMKDVNRSLQVHLQEIRSLKEVNQKLQEDNQELRELCCFLDDDRQKDRKVSREWQRFGRYTAGVLWRELGLYQQKLTELEAGQETLRAENAELKEIVLMLDEERSGAGSRSSIDSQSSLSGPVFLLEFVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LS50</accession>
    <name>A0A3B5LS50_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">small monomeric GTPase</fullName>
            <ecNumber evidence="5">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Phagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="5"/>
    <dbReference type="RefSeq" id="XP_027875656.1">
        <property type="nucleotide sequence ID" value="XM_028019855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LS50"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010639"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010763.1">
        <property type="protein sequence ID" value="ENSXCOP00000010639.1"/>
        <property type="gene ID" value="ENSXCOG00000008042.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114146089"/>
    <dbReference type="KEGG" id="xco:114146089"/>
    <dbReference type="GeneTree" id="ENSGT00940000163126"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000085">
        <property type="entry name" value="Putative ras-related protein rab-11a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="184"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="218" mass="24503" checksum="F1AAB42238B4C283" modified="2018-12-05" version="1">MGNRDDEYDFLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADNNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNNLSFIETSALDSTNVEEAFKNILTEIYRIVSQKQIAERSAHDESPGNNVVDISVPPTTDGQRGSKLQCCQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LS60</accession>
    <name>A0A3B5LS60_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Synuclein, beta</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the synuclein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LS60"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013650"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013818.1">
        <property type="protein sequence ID" value="ENSXCOP00000013650.1"/>
        <property type="gene ID" value="ENSXCOG00000010338.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183175"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903136">
        <property type="term" value="F:cuprous ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071542">
        <property type="term" value="P:dopaminergic neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008345">
        <property type="term" value="P:larval locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.700">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001058">
        <property type="entry name" value="Synuclein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13820:SF4">
        <property type="entry name" value="BETA-SYNUCLEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13820">
        <property type="entry name" value="SYNUCLEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01387">
        <property type="entry name" value="Synuclein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01211">
        <property type="entry name" value="SYNUCLEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118375">
        <property type="entry name" value="Synuclein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="113" mass="11828" checksum="0657570623B172CB" modified="2018-12-05" version="1">MDVFMKGLSKAKEGMAVAAEKTKEGVAVAAEKTKEGVMFVGTKAKDSVGTVAEKTTGAMGNIVAATGLVKKDEFPTDMNPEEYGQEAMEGQGEAMLEPEGEAYDESQQVTGTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSB1</accession>
    <name>A0A3B5LSB1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the distal-less homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027880129.1">
        <property type="nucleotide sequence ID" value="XM_028024328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSB1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014140.1">
        <property type="protein sequence ID" value="ENSXCOP00000013967.1"/>
        <property type="gene ID" value="ENSXCOG00000010571.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114148843"/>
    <dbReference type="KEGG" id="xco:114148843"/>
    <dbReference type="GeneTree" id="ENSGT00940000165079"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043009">
        <property type="term" value="P:chordate embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048731">
        <property type="term" value="P:system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000266">
        <property type="entry name" value="Distal-less homeobox 4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050460">
        <property type="entry name" value="Distal-less_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327:SF86">
        <property type="entry name" value="DISTAL-LESS HOMEOBOX 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327">
        <property type="entry name" value="HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="130"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="132"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="255" mass="27684" checksum="099A7D416149A83E" modified="2018-12-05" version="1">MTMSSISDALVSADPSKSAFLEFGGHGYPGHQQPPSGLAHNHYPVHSLHAVGGHGQHDGPFAAGASSYGRSLGYPPYHAPVSSHHPGTYLPYQHGGHGGALGHTRLEDTEHEKPTAVIENGEVRLNGKGKKIRKPRTIYSSLQLQALNQRFQQTQYLALPERADLAAKLGLTQTQVKIWFQNKRSKYKKIMKNGPCGAEGDHLAPPPPASSSPCSLWDISMAAKGPPVHSGGYMNNFGHWYSGHQQDTMPRTQMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSY0</accession>
    <name>A0A3B5LSY0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSY0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013930"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014102.1">
        <property type="protein sequence ID" value="ENSXCOP00000013930.1"/>
        <property type="gene ID" value="ENSXCOG00000010551.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158354"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000055">
        <property type="entry name" value="Homeobox protein Hox-A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050296">
        <property type="entry name" value="Antp_homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017995">
        <property type="entry name" value="Homeobox_antennapedia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45659">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45659:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00025">
        <property type="entry name" value="ANTENNAPEDIA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="186"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="188"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="79"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="152"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="263" mass="29288" checksum="775B6C3AF8F29346" modified="2018-12-05" version="1">MSSYFVNSFSGRYPNGSDYQLLNYGTNGAVSGSFRDPGTMHSGSFGYNYNGMDLTVNRNNTGGHFGAVREDARGFAPDARYRQTPNCSLSSPDPVPPSCATAGRDPLELKSPSPPSDRSATSSSGGNSLGKAGGAHFAEIEDAAQQQQQHQEPNATSSTPTPNECQTPQIFPWMRKLHISHDMTGPDGKRARTAYTRYQTLELEKEFHFNRYLTRRRRIEIAHALCLSERQIKIWFQNRRMKWKKDNKLKSMSLVTGGSAFHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTI9</accession>
    <name>A0A3B5LTI9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">small monomeric GTPase</fullName>
            <ecNumber evidence="5">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasmic vesicle membrane</location>
            <topology evidence="13">Lipid-anchor</topology>
            <orientation evidence="13">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Phagosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTI9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014155"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014332.1">
        <property type="protein sequence ID" value="ENSXCOP00000014155.1"/>
        <property type="gene ID" value="ENSXCOG00000010726.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157246"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030670">
        <property type="term" value="C:phagocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01867">
        <property type="entry name" value="Rab8_Rab10_Rab13_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000202">
        <property type="entry name" value="ras-related protein Rab-8A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="176" mass="20390" checksum="934F0A46DB32E8A9" modified="2018-12-05" version="1">MAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKKIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIKNWIRNIEEHASADVERMVLGNKCDVNDKRQVSKDRGEKLALEYGIKFMETSAKANINVENVSPFLFFLYFYYSENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVF2</accession>
    <name>A0A3B5LVF2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVF2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011809"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011945.1">
        <property type="protein sequence ID" value="ENSXCOP00000011809.1"/>
        <property type="gene ID" value="ENSXCOG00000008927.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163711"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050488">
        <property type="entry name" value="Ig_Fc_receptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481:SF64">
        <property type="entry name" value="FC RECEPTOR-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11481">
        <property type="entry name" value="IMMUNOGLOBULIN FC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="13"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="113"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="317" mass="35404" checksum="3FAA4A6079590952" modified="2018-12-05" version="1">MSDPGSWRSSVDPVLSVSPSWPNPGASVTLSCEGLEHQSEGWRFFWYKTVPDPSSHYSYELLPGSTNGTEQNSFIINGPTHTAGFVCRAGRGEPKFYTYYSKLKFIWSADPRPAASLSVNPDRVQHFRSDSVSLSCEGNSTEWRVMRFIERDRLLYFTCSSWGTMTGSTCTIDVSWNNDGVFWCESRSGETSNAINITVQDRFSAPLLVSPVHPVTDGDPVTLSCRDQKQKLLSNVFFYHNDKLLQNDSRGELNISAVSKSDEGFYKCQHSGKESPRSWMSVRGHLKINKPCALSPCVPYTGLPFITVTIALLVLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWF9</accession>
    <name>A0A3B5LWF9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cupin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWF9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015215"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015406.1">
        <property type="protein sequence ID" value="ENSXCOP00000015215.1"/>
        <property type="gene ID" value="ENSXCOG00000011530.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156867"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033749">
        <property type="term" value="F:histone H4R3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106140">
        <property type="term" value="F:P-TEFb complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050910">
        <property type="entry name" value="JMJD6_ArgDemeth/LysHydrox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480">
        <property type="entry name" value="ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480:SF32">
        <property type="entry name" value="BIFUNCTIONAL ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="18239" checksum="C28B07798AC95D71" modified="2018-12-05" version="1">MNHKSKKRIKEAKRSARPELKDSLDWMKHGYYENFDVSHQTVKDNVERVDALHLSPEEFILRFERPYKPVVLLNCQESWPAREKWTLERLKRKYRNQKFKCGEDNDGYSVKLKMKYYVEYLESTHDDSPLYIFDSKVWLLAPPTELAPPTQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWJ2</accession>
    <name>A0A3B5LWJ2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWJ2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013691"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013859.1">
        <property type="protein sequence ID" value="ENSXCOP00000013691.1"/>
        <property type="gene ID" value="ENSXCOG00000010362.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166251"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000021">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000039">
        <property type="entry name" value="Ubiquinol-cytochrome c reductase core subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF226">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="4">
        <location>
            <begin position="55"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="5">
        <location>
            <begin position="206"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="459" mass="49012" checksum="9E46F90038D4BDB8" modified="2018-12-05" version="1">IFRVLCSYFSQKRCYASARRGQTLTEPFSNVKPAQAAPFSPQDVQVSKLPNGLVIASVENYSPLSRVGVFIRAGSRHENSENLGVSHVLRLAANLTTKGASAFKICHGVEALGGSLSVTGSRENIIYSADCLRDDLGSLLDFLGNVTTAQEFRPWEVDELTSRVKIDRALAQQCPQISVIEKLHEAAYKNALSNSLYCPDFMVGRISSEQLHSFVKDNFTTGRMALVGVGVNHSVLKQVGEGLLSTRSGAGAPVAKAAYHGGELRVQNRDDLVHALVVSEGGVMGTAETNAFTVLQRILGASLHVKRGSSITSKLSQGIAKATAQPFDATAFNASYSDSGLFGVYTIAQANSAREVIRAAIAQVRGVAEGNVTEADIIRAKNQVKAEYLMSIESSEGLLEEIGAQALNTAAYQAPESVLHAIDAITRADVVKAARKFVDGKKTMAASGHLVNTPFVDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LX31</accession>
    <name>A0A3B5LX31_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nuclear receptor interacting protein 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LX31"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016483"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016690.1">
        <property type="protein sequence ID" value="ENSXCOP00000016483.1"/>
        <property type="gene ID" value="ENSXCOG00000012441.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007999"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <sequence length="1031" mass="110744" checksum="260153547278D271" modified="2018-12-05" version="1">MTHGEEPGPETHKDSAVLTYLEGLLMHPVVAGPGATAGGRSEASNSNQEQDNKVGGPLPQPNHDPMVPKTGTNGPKLGSSQHLKKARLLRSGAWNDPGNQRMSSPPVELNGQVGTLQNGALEASPHAGESTLLASLLQSFSSRLQSVAMSQHSNKPTGECSSPSKAPATDKEPLPVYGTASGRLKGLMRKSKLQNHNNTPYSRRGHSQDRPPESPRSAHSATPPAAPTVTDSVSCAERLKAVANMVKIRSSPAPSPKPSVACSQLALLLSSEAHLQQYSREHALKAQLSGRSASERLAAMANQQHGQDRRPPSVGGSSDTVSSLSTQNGMTTATITTTLPRTAATNPQSPSLQRGHSQNSPPSPPHAPSLPTREKRGFDTRPTRPPQTCSSLLLLLLNNHNNQKQLTKNGHLEDISGLLPPSGSSSVTSDSECSVHERSNTKDSSDAESSYSSCSPIDLSMRAPTQPPTTSFSPSSMAVSSVSYPILSTVSKNKEPEMNPDLKSHPKVTLMQLLLERRNNEMANKSTGRQDSPLDITMANMSRNQQKGSVTWEEGPTKSPIERPAGPSQSVYSLSRDTGPAMSPYSYPSPHVQSSPLDLCKSKTFPADKASEPAFSASKLLQNLAQCGTTSPPPPISSGKGASQELNDSRPHALLERLNTPVNRTTPTPLSDGASGSSTPFSRKEPSPPSSQIENLLERRTVLQLLLGTGSTGAIAGRKERPSGSGHGSAEMEELPEDVGSSLTKSEDLSGKKRLSSYGKNSPLSDSQQDLKTEPRPAEVIAKYGLLSQLLKQQTATYYSSAATMQPEPQSRQVKEEHRDYPNPSPKRRRLCSEHTGSLNSISSPRAVDSGNANMCASSTLQADPEQQRGLKEEELPPRSPMSETFTRESRGFNVLKQLLLSDNCLKELSQQPRQGPSPSILQTTGKEPESPIRWSSQENDGMDEGCESNPDSPRLSRSNPILYYMLQKGNIQLRKEVPDQAEGARHAIRVKEEPVSDMHTYERSLSSTPQSPTHNDKHSHEGQGLSQSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXK0</accession>
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        <recommendedName>
            <fullName evidence="2">KIND domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="32473.ENSXCOP00000015872"/>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
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    <sequence length="207" mass="22818" checksum="F0F405F84EDCEEBD" modified="2018-12-05" version="1">MSSTFVTLAEVLEARGGPLLEEEIWSLLLGAAECLMDVSYKNNMCSIISPTSLLLSATGSLAFKNCGLSDEVSAFAAPEMLQDRAGSTKPATDRQMLVYSLGMTLYWSVDFQLPQNQPVQLSDHLNSILLSMCEDLAHRRVNLTSILEACESQHKASNLLPPTKVVRQLVEEVFHESVDGSLPDSNIPLSGRSQMIRERLHSEYAFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXP4</accession>
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        <submittedName>
            <fullName evidence="8">GRAM domain containing 1c</fullName>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
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        <property type="entry name" value="Cholesterol_transport"/>
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        <property type="entry name" value="VASt"/>
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        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
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        <property type="entry name" value="PROTEIN ASTER-C"/>
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    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="433"/>
            <end position="456"/>
        </location>
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    <feature type="domain" description="VASt" evidence="7">
        <location>
            <begin position="212"/>
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        </location>
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            <begin position="187"/>
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        <location>
            <begin position="404"/>
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            <begin position="187"/>
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            <begin position="404"/>
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            <dbReference type="ARBA" id="ARBA00004167"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="507" mass="59368" checksum="F465A1EB4D476CB7" modified="2018-12-05" version="1">AVPQSPLPSYKQRVEEFKRLFKELPDSEILILYPCALHRDILLQGRLYLSENWICFYSYVFRGTKITLSLKDITTMSREKTARLIPNAIQICTHTEKYFFASFSAREKSYLSVFRIWQNTLLDKLWQMIKQHYGYDLGLNNEEMESLQLQDESLNTPSLHRTPTLPLERHLAERLAKCSTQSLDLNANENLLEQSGSETNEEEDRMDKSEEPGRLYLNKVFHMSASKMFALLFTDSSFTQRFMEIRRITDPSSTAWQKDDSGNDKRVMNYTITINNPLIGKFSTATENQTLSKESREGQYYQVDAVVHTHDVPYHDHFYTQNRYYIIRVSKRKCRLVYSDVKYSKPTFGLIKTFITKNSWSGIEDYFRQLAELLEEEAEMNQAVGESGKAGLRRRRRTFSRTLLDHGKPGKQSVRDPDDRDGNMPQRWSTTAIVLSMSVLLILMVVNLGLFFKLWAMEDVAHRMYLTTKHRLRERMETMQLLKTVLQDSILLLEQLRTSLVVLQQNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXZ4</accession>
    <name>A0A3B5LXZ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the even-skipped homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXZ4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012843.1">
        <property type="protein sequence ID" value="ENSXCOP00000012689.1"/>
        <property type="gene ID" value="ENSXCOG00000009586.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000167564"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052002">
        <property type="entry name" value="Even-skipped_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46294:SF3">
        <property type="entry name" value="EVEN-SKIPPED-LIKE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46294">
        <property type="entry name" value="SEGMENTATION PROTEIN EVEN-SKIPPED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="11"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="13"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="216" mass="24715" checksum="3581ECB0D26639A0" modified="2018-12-05" version="1">EHRTNLCGSLDPSRRHRTAFTRDQVSRLEQEYRNESYVSRARRCELAAALNLPETTIKVWFQNRRMKDKRQRHSLPWSHPLINPLGTLLMGHASPSSNLIYPFSTSQLPHLPFQHYCPLTFSGMINGPHSALMKQPERFSLSQQHNKPGEGPPTAVLVYPSPGVMHHHVSSHCSFCPQWGQQDLHKVQWEMEGLSHANNSAAKKQTGSLQQREEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LY96</accession>
    <name>A0A3B5LY96_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="14">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 14">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LY96"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014316"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014493.1">
        <property type="protein sequence ID" value="ENSXCOP00000014316.1"/>
        <property type="gene ID" value="ENSXCOG00000010856.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153838"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05052">
        <property type="entry name" value="PTKc_Abl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09935">
        <property type="entry name" value="SH2_ABL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11850">
        <property type="entry name" value="SH3_Abl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000070">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000010">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000037">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000004">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035837">
        <property type="entry name" value="ABL_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015015">
        <property type="entry name" value="F-actin-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08919">
        <property type="entry name" value="F_actin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00808">
        <property type="entry name" value="FABD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0519">Myristate</keyword>
    <keyword evidence="4 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="1 12" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="2 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 14" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="93"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="16">
        <location>
            <begin position="159"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="274"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="568"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="636"/>
            <end position="738"/>
        </location>
    </feature>
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        <location>
            <begin position="757"/>
            <end position="919"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="940"/>
            <end position="1071"/>
        </location>
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        <location>
            <begin position="636"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="664"/>
            <end position="678"/>
        </location>
    </feature>
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        <location>
            <begin position="699"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="779"/>
            <end position="788"/>
        </location>
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        <location>
            <begin position="831"/>
            <end position="847"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="873"/>
            <end position="886"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="892"/>
            <end position="902"/>
        </location>
    </feature>
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        <location>
            <begin position="940"/>
            <end position="949"/>
        </location>
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        <location>
            <begin position="1016"/>
            <end position="1026"/>
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        <location>
            <begin position="1035"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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            <dbReference type="ARBA" id="ARBA00022707"/>
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            <dbReference type="ARBA" id="ARBA00022777"/>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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            <dbReference type="ARBA" id="ARBA00023288"/>
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            <dbReference type="ARBA" id="ARBA00051245"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
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        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1177" mass="127663" checksum="F39099DC8DF434B7" modified="2018-12-05" version="1">MGQQVGRVGEVTSTALQPPQPHASQPHQGKGSRGSVAGRRPREPGCSTMMPPVRTTATNAPDPGINIFTQHSEVLTEAVRWSSKENLLGATESDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNQNGEWSEVRSKNGQGWVPSNYITPVNSLEKHSWYHGPVSRSAAEYLLSSLINGSFLVRESESSPGQLSISLRYEGRVYHYRINTATDGKVYVTSESRFATLAELVHHHSTVPDGLVTTLHYPAPKCNKPTVYGVSPIHDKWEMERTDITMKHKLGGGQYGEVYVGVWKKYNLTVAVKTLKEDTMEVEEFLKEAAVMKEVKHPNLVQLLGVCTLEPPFYIVTEYMPHGNLLDYLRDCEKSEVNAVALLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHVVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNTFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYDLLEKGYRMEQPEGCPPKVYELMRACWQWSPLDRPSFAEIHQAFETMFHDSSISEEVAEELCKTAHSVSHDMPLLPSKSRTLHKHTENKENIEGGLDGWTSLFGGDGRSSSSPALPRKQQPRDKSPSSLLEDAQDIGTFTRDRKTGFFSSFIKKKSTSSSSSSSSPSSQVQQNLPTPPKRSSSFREMETQPHKKYEPTADFSAPPPLPQSDSLGGFSASPSHSHGEATQSQSRCCGAAFGQKPSAGGLGSQLTSGSSWSGLAGFFTPRLIKKTLGLRTGKTASSDESGIVGGPKPFPRSNSTSSMSSGLPDLERMALTLPRNRSAKSPLERTASTTSQLENGAARPSENLLRRMDEGTAQIRDRPKAKLLPRGTAVGVRAPGVGGEVGESESLSRVRDSREEGGGLDRQQSWSSPSKNSTSTHNHKVPVLISPTLKHSPADVHLVGLDSQGNRFKLLSEHQGERDRPRLVKPRCAPPPPPTLRGLQQSYSCDSEDQVSGAPVEVNGDAVKGQRSGRMSGGATTGRPSVPPPQVPPASSSSSCPASTNTTPTKMANGAATTASSSSQTAPSAGSKVALRRTRQQVERVPLERASREALLECAESLSSVLSSSSDSPSSSQVLDAGHQLLDCCSGYVDCIPQMRNKFAFREAVGKLELSLQELRASSSGGGCLSSVGSNTVLDNLHNCIKEISDVVQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0Q8</accession>
    <name>A0A3B5M0Q8_9TELE</name>
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        <recommendedName>
            <fullName evidence="1">FHA domain-containing protein</fullName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <begin position="23"/>
            <end position="73"/>
        </location>
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            <dbReference type="PROSITE" id="PS50006"/>
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    <sequence length="136" mass="15645" checksum="BFC66819A44AAEAF" modified="2018-12-05" version="1">MSVTSWFLVSNGGTRHRLPREMIFVGRDDCELMLQSRSVDKQHAVINYEPGSDEHKVKDLGSLNGTFVNDVRIQEQMYITLKLEDKLRFGYDILSLFHFLLCEASHIQWTHSHPHSDYLSQSEAHSYSNATAIGYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="1">Ig-like domain-containing protein</fullName>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="15"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="113" mass="12469" checksum="DA824F7728C73029" modified="2018-12-05" version="1">MNLMMKQTIIQSLGSQITVTQQPAAVRADLGRSATITCRTSQNVYNNNYLHWYQQKDGQTPKLLIYYTSTKASGTPSRFSGSGSNSDFTLTISEVQAEDAAVYYCQKSNSSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2R6</accession>
    <name>A0A3B5M2R6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Heat shock 70 kDa protein 12A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Adapter protein for SORL1, but not SORT1. Delays SORL1 internalization and affects SORL1 subcellular localization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with SORL1 (via cytosolic C-terminus); this interaction affects SORL1 internalization and subcellular localization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M2R6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018398"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018630.1">
        <property type="protein sequence ID" value="ENSXCOP00000018398.1"/>
        <property type="gene ID" value="ENSXCOG00000013857.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154551"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11735">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000061">
        <property type="entry name" value="Heat shock protein family A (Hsp70) member 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000011">
        <property type="entry name" value="Heat shock protein family A (Hsp70) member 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026685">
        <property type="entry name" value="HSPA12A_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187">
        <property type="entry name" value="ALPHA KINASE/ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187:SF46">
        <property type="entry name" value="HEAT SHOCK 70 KDA PROTEIN 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="655" mass="73352" checksum="39DFC90149AECF39" modified="2018-12-05" version="1">MANPSLSKSGGDPGITPLSPSHIQQSDTDQVQSGPSFVVVVAIDFGTTSSGYAYAFTKEPECIHTMRRWEGGDPGVSNQKTPTTILLTPDRKFHSFGYAARDFYHDLDPSESKHWLYLEKFKMKLHTTANLSIDTEIHAANGKRVKALDIFAYALAFFKEQALKELSDQTGSEFDNSNVRWVITVPAIWKMPAKQFMREAAYKSALVSRENPEQLIIALEPEAASIYCRKLRLHQMVDLGTQTTQNGFSPNDNVGSGMTQAKEHARRNRQSRTFLVENVIGELWSELEEGDRYVVMDCGGGTVDLTVHQIRLPEGHLKELYKASGGPYGSIGVDYEFEKLLCKIFGQDFIDQFKIKRPAAWVDLMIAFESRKRAAAPERTNPLNINLPFSFIDYYKKFRGHSVDHALRKSNVDFVKWSSQGMLRMSPDAMNTLFKPTIDHIIQHLTELFEKPEVSDIKFLFLVGGFAESALLQQAVQNMLQGRSRIIIPHDVGLTILKGAVLFGLDPSVIKVRRSPLTYGVGVLNRFVEGKHPPEKLLIKDGTRWCTDVFDTFIAADQSVALGEMVKRSYTPAKPSQQVIVIHVYCSEKERAGFISEPGVRKCGTLRLDVSGTESSAPRREIQTLMQFGDTEIRAMAIDVSTGRTVKASIDFLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3L7</accession>
    <name>A0A3B5M3L7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Coiled-coil domain containing 59</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027899474.1">
        <property type="nucleotide sequence ID" value="XM_028043673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3L7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018057"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018284.1">
        <property type="protein sequence ID" value="ENSXCOP00000018057.1"/>
        <property type="gene ID" value="ENSXCOG00000013601.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114160828"/>
    <dbReference type="KEGG" id="xco:114160828"/>
    <dbReference type="CTD" id="29080"/>
    <dbReference type="GeneTree" id="ENSGT00390000006546"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013730">
        <property type="entry name" value="Fyv7/TAP26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15657">
        <property type="entry name" value="THYROID TRANSCRIPTION FACTOR 1-ASSOCIATED PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15657:SF1">
        <property type="entry name" value="THYROID TRANSCRIPTION FACTOR 1-ASSOCIATED PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08524">
        <property type="entry name" value="rRNA_processing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01854">
        <property type="entry name" value="BR22PROTEIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="250" mass="29586" checksum="3E5BBDB8A41A4C69" modified="2018-12-05" version="1">MAPTNQKRKNDKPQIKDGNFRKERQASGGVNKKKKWIPHNKMFEGSVKEGQGFAFKKKEKVRHEYNKLLRRERKKNPKADVVYKEEYPQHLKHLYLAEAEQLRKQAWENRVNRSKLRMKGQMKDGTENNADVPDTDFAGGSELMDSASGNPEPTTSEQESVPISNRMRKKMQRKTSYQKTKEEFENVQEKRRLKKEAYLKNKQQREEAIQRYKQKKVETFRLLSQKTKKGQPNLNVQMEYLLQKIQGTDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5Y9</accession>
    <name>A0A3B5M5Y9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription factor HES-5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Hairy and enhancer of split 5</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Transcriptional repressor of genes that require a bHLH protein for their transcription. Plays an important role as neurogenesis negative regulator.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027857596.1">
        <property type="nucleotide sequence ID" value="XM_028001795.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027857597.1">
        <property type="nucleotide sequence ID" value="XM_028001796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M5Y9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019093.1">
        <property type="protein sequence ID" value="ENSXCOP00000018857.1"/>
        <property type="gene ID" value="ENSXCOG00000014192.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114134925"/>
    <dbReference type="KEGG" id="xco:114134925"/>
    <dbReference type="GeneTree" id="ENSGT00940000163346"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097150">
        <property type="term" value="P:neuronal stem cell population maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11461">
        <property type="entry name" value="bHLH-O_HES5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000033">
        <property type="entry name" value="Transcription factor HES-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003650">
        <property type="entry name" value="Orange_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158457">
        <property type="entry name" value="Orange domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51054">
        <property type="entry name" value="ORANGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="15">
        <location>
            <begin position="19"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Orange" evidence="16">
        <location>
            <begin position="88"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="121"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00081413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="159" mass="18381" checksum="45E95E651A30F923" modified="2018-12-05" version="1">MAPTIPAAMTNSQEHLTLNHKLRKPLVEKLRRERINSSIEQLKSLLGPEFLKQQPDSKLEKADILEMTVCFLRRLQQQQHPHLNAGAADQGYSRCFQEVVHFLSKDDLKTQSQRRLLNHMNQLQSSSERNLKDNFPINSTTQASIKKKSPANSSLWRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6H1</accession>
    <name>A0A3B5M6H1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">26S proteasome non-ATPase regulatory subunit 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">26S proteasome regulatory subunit RPN5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PSMD12</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD12, a base containing 6 ATPases and few additional components. Interacts with ERCC6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the proteasome subunit p55 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6H1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019818"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020064.1">
        <property type="protein sequence ID" value="ENSXCOP00000019818.1"/>
        <property type="gene ID" value="ENSXCOG00000014873.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153510"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000159">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054559">
        <property type="entry name" value="PSMD12-CSN4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040134">
        <property type="entry name" value="PSMD12/CSN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040896">
        <property type="entry name" value="RPN5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12/COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22241">
        <property type="entry name" value="PSMD12-CSN4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18098">
        <property type="entry name" value="RPN5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="PCI" evidence="10">
        <location>
            <begin position="222"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00075108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="436" mass="50648" checksum="032FD2DCE51999D3" modified="2018-12-05" version="1">MSEERSERSDGRIVKMEVDYSSTVDERLPECEKMAKASDMVSTSRILVAVVQMCYEAKDWDALNENIMLLTKRRSQLKQAVAKMVQECYKYVDAVTDLNIKLRLIDTLRTVTAGKIYVEIERARLTKTLANIKEQSGDVKEAAAILQELQVETYGSMEKKEKVEFILEQMRLCIAVKDYIRTQIISKKINTKFFQEEGAEELKLKYYNLMIQVDQHEGSYLSICKHYKAIYDTPSILEDSSKWQQALKSVVLYVILSPYDNEQSDLVHRISADKKLEEIPKYKDLLKQFTTMELMRWASLVEDYGKELREGSSESPATDVFSYSEEAEKRWKDLKNRVVEHNIRIMAKYYTRITMKRMAGLLDLSVDESEEFLSSLVVNKTIYAKVDRLAGIINFQRPKDPNDLLNDWSQKLNSLMSLVNKTTHLIAKEEMIHNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M783</accession>
    <name>A0A3B5M783_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Histone deacetylase</fullName>
            <ecNumber evidence="3 12">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the histone deacetylase family. HD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="3 12"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M783"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019330"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019568.1">
        <property type="protein sequence ID" value="ENSXCOP00000019330.1"/>
        <property type="gene ID" value="ENSXCOG00000014540.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157440"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10162">
        <property type="entry name" value="ClassIIa_HDAC4_Gln-rich-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000002">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046949">
        <property type="entry name" value="HDAC4/5/7/9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024643">
        <property type="entry name" value="Hist_deacetylase_Gln_rich_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45364:SF13">
        <property type="entry name" value="HISTONE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45364">
        <property type="entry name" value="HISTONE DEACETYLASE 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12203">
        <property type="entry name" value="HDAC4_Gln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037911">
        <property type="entry name" value="HDAC_II_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0678">Repressor</keyword>
    <keyword evidence="10 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Histone deacetylase glutamine rich N-terminal" evidence="18">
        <location>
            <begin position="44"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Histone deacetylase" evidence="17">
        <location>
            <begin position="639"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="109"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="209"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="483"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="536"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="109"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="210"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="235"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="268"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="512"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="761"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="631"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="633"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="639"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="709"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="site" description="Contributes to catalysis" evidence="15">
        <location>
            <position position="934"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR037911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR037911-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037911-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR037911-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF12203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1042" mass="114643" checksum="141FA97E1E1474E4" modified="2018-12-05" version="1">MSVDVSTAALPSQVPSAASAILPMDLRIVDHSHHHHQQPPFGRQDQHLQQELVALKHKQELQRQLLIAEFHRQHEQLSRQHEVQLQEHIKHQQDLLALKHQQELLEHHRKMEQQHHEQQLEKQQREQKLQQLKNKERGQESAVASTEVKMRLQEFVLNKKKALAQRNLNHCMPSDPRYWYGKTQHSSLDQSSPPQAALSAYTHPVLGAFDSKDDFPLRKTASEPNLKRRSWLKQKVTERRSSPLLRRKDGPITTAKKRSLDRADSACNSAPGSGSSSPNNSSSNIPSENGIPISTSPGEASLIQRLATRDGSLSQLSLYTSPSLPNITLGLPASGPANSVVSGHQDGETHLALPALQPGIPLASAFLPTSHLPSYLASSALDRDGPGGGAAGHNPLLQHMVLMEQSHSSMVGLGGLPLQSPTVSKLVRGHRPLGRTQSAPLPQQPCGQAQALQQLVVQQQHQQFLEKHKQLFQQQHIINKVSHHVQSSVPGRQHQSHPEETEEELREHQGLTSSSSSSSTSSGPETGLIRGLVIKQEPPDLQEQEEREQRDQRERQQAMLLEQQRIHQLRSYRASMEAAGLPVSLAAHRPLSRTQSSPASASFPIVVSEPPVKHRFTTGLVYDSLMQKHQCICGNATIHPEHAGRIQSIWSRLQETGLRAQCECIRGRKASQEELQTVHSEVHVQLYGTNPLRQKLECSVNPMFVRLPCGGIGVDSDTIWNEIHSSTAARLAVGSVVELVFKVATGELKNGFAVVRPPGHHAEESTPMGFCYFNSVAIAAKLLQQRLNVSKILIVDWDVHHGNGTQQAFYADPSVLYLSLHRYDDGNFFPGSGAPDEVGSGAGVGFNVNMAFTGGLEPPMGDAEYLAAFRTVVMPIANEFAPDVVLVSSGFDAVDGHAPPLGGYKLTAKCFSYLTRQLMGLAGSRLVLALEGGHDLMAICDASEACISALLGNELDPIPYEVLQQKPNANAVRSMEKVIEVQSKYWRSLQRSASTLGWSLNEALQRDVEEAETVSAMASLSVANKHIGKRAEEEPMEEEAPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7D8</accession>
    <name>A0A3B5M7D8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Akt interacting protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7D8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017251"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017472.1">
        <property type="protein sequence ID" value="ENSXCOP00000017251.1"/>
        <property type="gene ID" value="ENSXCOG00000012999.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010125"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23814">
        <property type="entry name" value="UEV_AKTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000030">
        <property type="entry name" value="AKT-interacting protein-like isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="76"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="289" mass="33195" checksum="35E6B280955AEB38" modified="2018-12-05" version="1">MNLNPFWSMSANTSRKRADEEQSGHTDQRASPARTHFGKKVLPPIPKNATPITKPPSMVSAAHSANGTHASYGPFYLEYSLLAEFTLVIKQKLPGIYVQPSYKSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGECPKLVFDIPVFHPLVDPVSGELDVRRAFTKWRRNHNHIWQVLMYARTVFYKINTTEPLNPEAAVLYEKDVQLFKSKVVDSVKLCNSHLFDQPKIDDPYAISFSPWNPAVHEEAKERMFTYKVSYMNILKSTELYSVKVWIRYLHVICSAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7Z9</accession>
    <name>A0A3B5M7Z9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Band 7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7Z9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019620"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019865.1">
        <property type="protein sequence ID" value="ENSXCOP00000019620.1"/>
        <property type="gene ID" value="ENSXCOG00000014734.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014666"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03406">
        <property type="entry name" value="SPFH_like_u3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033294">
        <property type="entry name" value="Erlin1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15351">
        <property type="entry name" value="ERLIN (ER LIPID RAFT ASSOCIATED PROTEIN) HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15351:SF4">
        <property type="entry name" value="ERLIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="12" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="10" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017309937" description="Band 7 domain-containing protein" evidence="13">
        <location>
            <begin position="18"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="14">
        <location>
            <begin position="21"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="312" mass="34725" checksum="91AA35B31D10E023" modified="2018-12-05" version="1">MAQWGAIFSIVAALGGAALLASVHKIDEGHTGVYYRGGALLTATSGPGFHLMLPFITTYKSVQECLSSVWENPLFFLFSVYDIVRNFTADYDKALIFNKVHHELNQFCSVHSLQEVYIGLFDQIDENLKLTLQEDLTSMAPGLIIQAVRVTKPNIPESIRRNYELMESEKTKLLISQQTQKVVEKEAETERIKAVIEAEKVAQVAEIKFGQKIMEKETEKKISEIEDGAFLARQKAKADAEFYTALRVAEANKLKLTPEYLQLMKYKAIAANSKIYFGNDIPQMFVDSGSAGSSIKASAALDVIGDQVIDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M960</accession>
    <name>A0A3B5M960_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Centromere protein S</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TAF9 family. CENP-S/MHF1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M960"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017811"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018035.1">
        <property type="protein sequence ID" value="ENSXCOP00000017811.1"/>
        <property type="gene ID" value="ENSXCOG00000013420.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000174411"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071821">
        <property type="term" value="C:FANCM-MHF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029003">
        <property type="entry name" value="CENP-S/Mhf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22980:SF0">
        <property type="entry name" value="CENTROMERE PROTEIN S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22980">
        <property type="entry name" value="CORTISTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15630">
        <property type="entry name" value="CENP-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="44"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="80" mass="9236" checksum="11F5307D5EF5D4F0" modified="2018-12-05" version="1">SLHFETPVTVVIFAKDLEAFAHAKRSTVSAEDVKLLARRSRALSNHIQNKSEELAQEQRESRKKSTVKRKSRETEEESRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9G0</accession>
    <name>A0A3B5M9G0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9G0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021165.1">
        <property type="protein sequence ID" value="ENSXCOP00000020908.1"/>
        <property type="gene ID" value="ENSXCOG00000015654.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="135" mass="15542" checksum="16E783BECEBFB15C" modified="2018-12-05" version="1">MVFNSLLMCLKYHSFSALWCTNYAVTDAELFIFVLANLFLSVLCVFLYYTKKNLFLVSLFHNTVLCNSYFVKLKINSTDNFLAEKDKKKQLTRLDEGIYQLSVSETKCMQKEDGSRADCIEVTVAARACSLFLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9W8</accession>
    <name>A0A3B5M9W8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9W8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016773.1">
        <property type="protein sequence ID" value="ENSXCOP00000016559.1"/>
        <property type="gene ID" value="ENSXCOG00000012498.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157147"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03391">
        <property type="entry name" value="PAP2_containing_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF17">
        <property type="entry name" value="INACTIVE PHOSPHOLIPID PHOSPHATASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="3">
        <location>
            <begin position="148"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="283" mass="30845" checksum="03E23030F7C8C48F" modified="2018-12-05" version="1">MPGSQTRSRPRDRNNILNRPEFMSLNQPVRRETSGEGRSRRTATRRPSQDSSDGAPSGDGVPKDGSRWPEQDCMQLNPSFRGIAINSLLAIDINLSRRLGMCMVGPRGPGAPLRPMVALLAFSGHALPWLCGTLLCLWRSDSLAGQEVLVNLLLALLLDLMTVAGVQKLVRRHSPWNAPPGFLDRVALDSYSFPAAHASRAVVVSRFLLNHLVLAVPLRILLYLWAFLVGVSRVLLGKHHLSDVGCGLALGFLLFSLVESVWLDSAACQALLAVGSLHWAPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAH9</accession>
    <name>A0A3B5MAH9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Vitamin K-dependent protein C</fullName>
            <ecNumber evidence="16">3.4.21.69</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Anticoagulant protein C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Autoprothrombin IIA</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Blood coagulation factor XIV</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Exerts a protective effect on the endothelial cell barrier function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Degradation of blood coagulation factors Va and VIIIa.</text>
            <dbReference type="EC" id="3.4.21.69"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.69" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAH9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021253"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021513.1">
        <property type="protein sequence ID" value="ENSXCOP00000021253.1"/>
        <property type="gene ID" value="ENSXCOG00000015907.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160104"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000011">
        <property type="entry name" value="Coagulation factor X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF20">
        <property type="entry name" value="LOW QUALITY PROTEIN: TRANSMEMBRANE PROTEASE SERINE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Peptidase S1" evidence="21">
        <location>
            <begin position="6"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00040219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="97" mass="10537" checksum="3268612B694A7714" modified="2018-12-05" version="1">LPTSGKVDVRLVSEEACVRSYGYLVTPRMLCAGYRSGGKDACQGDSGGPLVCQEPSGRWFLAGVVSWGKGCGRPDYYGVYTRITRLTDWIKQVISSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAV7</accession>
    <name>A0A3B5MAV7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Potassium voltage-gated channel, subfamily G, member 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAV7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020717"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020969.1">
        <property type="protein sequence ID" value="ENSXCOP00000020717.1"/>
        <property type="gene ID" value="ENSXCOG00000015529.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159686"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18421">
        <property type="entry name" value="BTB_POZ_KCNG1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000005">
        <property type="entry name" value="potassium voltage-gated channel subfamily G member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000024">
        <property type="entry name" value="Potassium voltage-gated channel subfamily G member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000019">
        <property type="entry name" value="Potassium voltage-gated channel, subfamily G, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003969">
        <property type="entry name" value="K_chnl_volt-dep_Kv6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF88">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY G MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01492">
        <property type="entry name" value="KV6CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="303"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="380"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="413"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel tetramerisation-type BTB" evidence="15">
        <location>
            <begin position="68"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="14">
        <location>
            <begin position="230"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="517" mass="59058" checksum="E69A864C37D000B9" modified="2018-12-05" version="1">MTLLAGDGSDYDYSALSCASDTSLNQPPQQEAEARKGAFYKRAQLALERTTVPDNTALSGARKIHAIINVGGLRYQMPWTTLEDFPLSRLGQLHLCSSFDEIMRICDDYDVAHNEFFFDRSPCAFRTILTFLRAGKLRSLREMCALSFREELLYWGVPEESLEWCCRRRMLQRVKEFEAMERAEEEDELLEDLLDSENGHRERTAETRINWCMNKLRDMVERPHSGLPGKIFACLSVLFVTITAINLSISTMPAMREEEEAGTCSKMCYNIFIVETVCVAWFSLEFTLRFIQDRSKLTFFRKPLNLIDVVAILPYYITLLVDGSSNVEKRLSSGSSYLDKVGLVLRVLRALRILYVMRLARHSLGLQTLGLTARRCTREFGLLLLFLCVAIALYSPLLYLIENEMAATQEFTSIPATYWWAVITMTTVGYGDMVPRSIPGQVVALSSILSGILLMAFPVTSIFHTFSRSYVELKQEQQRILQRRTHFLLRSRMAGLGSNLSLESDLLFPIGEEWKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBC4</accession>
    <name>A0A3B5MBC4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phagosome assembly factor 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PHAF1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBC4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016999"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017218.1">
        <property type="protein sequence ID" value="ENSXCOP00000016999.1"/>
        <property type="gene ID" value="ENSXCOG00000012818.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153528"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005373">
        <property type="entry name" value="PHAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039156">
        <property type="entry name" value="PHAF1/BROMI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465:SF2">
        <property type="entry name" value="PHAGOSOME ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465">
        <property type="entry name" value="UPF0183 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03676">
        <property type="entry name" value="PHAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="413" mass="46684" checksum="C1CF818ED0E9F2EF" modified="2018-12-05" version="1">MLDLEVVPERSLGNEQWEFALGMPFSQAISILQKHCRVIKNVQVLYSEQTPLSHDLILNLTQDGIKLLFDATNQRLKVRMARLAQISNSHCIHFNSQAITPTIEQIDQSFGATHPGVYNAAEQLFHLNFRGLSFSFQLDSWNEAPKYEVRMDRFPSIPHGAMVKRMHIYTGNNLQETKAPAMPLACFMGNIFAESVDVLRDGAGPLGLKLRLLTAGSGLGVMADAKVRYVERSIFFGDSCQDVLGTLGSPHKVFYKSEDKMKIHSPSPHKQVPSKCNDYFFNYFTLGVDILFDSTTHLVKKFVLHTNFPGHYNFNIYHRCDFKIPLVIKKGVFVFLRQWDQIQELLGHPMEKPVVLHRSSSANNTNPFGSTFCFGLQRMIFEVMQNNHIASVTLYGAPRTTSQARPESSASSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBL0</accession>
    <name>A0A3B5MBL0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBL0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020960"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021218.1">
        <property type="protein sequence ID" value="ENSXCOP00000020960.1"/>
        <property type="gene ID" value="ENSXCOG00000015689.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154313"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="137" mass="15616" checksum="30FCE805F3F0A780" modified="2018-12-05" version="1">MENNPTIYLTMRGTVCVGCRSLRPGRHIEDDNIVPELAHIHPRERPDWEETISAMARSAEIPELRAEPLIRSSSSSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGTIQSEARCPGSSNWRHSWHFYTSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCK1</accession>
    <name>A0A3B5MCK1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA repair protein RAD51 homolog 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCK1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021978"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022245.1">
        <property type="protein sequence ID" value="ENSXCOP00000021978.1"/>
        <property type="gene ID" value="ENSXCOG00000016419.1"/>
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    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033063">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000707">
        <property type="term" value="P:meiotic DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19492">
        <property type="entry name" value="Rad51C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052093">
        <property type="entry name" value="HR_Repair_Mediator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46239:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46239">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 3 RAD51C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005856">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
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    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="9">
        <location>
            <begin position="84"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="331"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="332"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="348"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="361" mass="39089" checksum="8B548B8A6B947F91" modified="2018-12-05" version="1">KRAAVSAVRMKRPVSSLSLGSSLKGKLVRAGFQFTTDLQDLNPEHLSAEAGVSQQEALEALQAASSGHGVAPVTALELLQKEEEFRSIVTFCSQLDEALGGGVPVGKVTEICGAPGVGKTQLCLQLAVDVQVPAVFGGLGGQVLFVDTEGGFQVQRLVDLAGAAVRHCSLLAEDEEQRVAMTTFTVETILSNVFLVRCRDYVELLAELHLLADFLSNRPRLRLLVIDSVAFPFLRLHDDLSQRTRLLQGLGLRLIAAATGHNIAVVTTNHMTTRLHSSQAQLAPALGDVWGHAPSIRLLLRWEESRRLASIVKSPGHMDASVQYQITYQGFRDADQSERPPSKRPRSQTDQSASSRKNTGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDN2</accession>
    <name>A0A3B5MDN2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDN2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017809"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018033.1">
        <property type="protein sequence ID" value="ENSXCOP00000017809.1"/>
        <property type="gene ID" value="ENSXCOG00000013418.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161860"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014704">
        <property type="term" value="C:intercalated disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Potassium voltage-gated channel subfamily a member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000012">
        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 10"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003972">
        <property type="entry name" value="K_chnl_volt-dep_Kv1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF250">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY A MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01496">
        <property type="entry name" value="SHAKERCHANEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="341"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="15">
        <location>
            <begin position="51"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="487" mass="55799" checksum="2C90166492CF17B6" modified="2018-12-05" version="1">MKAVCNCKEKRRVFKARRTRNIGTGSRVWPWAPNFPPEKRGGHKGNKESNERVLINIAGLRYETQLGTLNQFPDTLLGDPAKRIKYFDPLRNEYFFDRNRPSFDGILYFYQSGGKIRRPVNVSIDVFADEIRFYQLGEEAMERFREDEGFIKEEEKPLPQNEFQKQVWLIFEYPESSSPARGIAIVSVIVITISIITFCLETLPEFRDERELAVSSRLDNSTAPRPSLTFTDPFFIIETTCVIWFTFELIVRFFACPSKSEFSKTIMNIIDIMSIMPYFITVGTELAEQQGQEHQNGQQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILFSSAVYFAEADEPESHFSSIPDAFWWAVVTMTTVGYGDMRPVTVGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETDQDQSKTDRLLSGNFPVALRNRLLSQNFHVAKRNRQVSFPNLGLRLKNFQVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEM2</accession>
    <name>A0A3B5MEM2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ATP-binding cassette, sub-family D (ALD), member 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEM2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022005"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022272.1">
        <property type="protein sequence ID" value="ENSXCOP00000022005.1"/>
        <property type="gene ID" value="ENSXCOG00000016443.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182955"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF71">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY D MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="254" mass="28366" checksum="3090712535074178" modified="2018-12-05" version="1">MDGRLSNPDQSLTEDIVMFSASVAHLYSNLTKPILDVVVTCYTLLRTAQSKGANTTWPSAIAGLVVALTAKVLRSCSPHFGKLVAEEAKRKGDLRYMHSRIIANSEEIAFYAGHKVELSQLQRSYASLSSQIHQILLKRLWYVMLEQFLMKYVWSASGLVMVAVPIITATGYSKNGKLRPPAVGRRLRRQLVTSDPWCADSEEVKRAAMVMKEEDLVSERTQAFTTARNLLNAAADAVERIMSSYKEVSMPRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MET2</accession>
    <name>A0A3B5MET2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Platelet glycoprotein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="31">Glycoprotein IIIb</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">PAS IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">PAS-4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">Platelet glycoprotein IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="34" type="primary">CD36</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(9Z)-octadecenoate(out) = (9Z)-octadecenoate(in)</text>
            <dbReference type="Rhea" id="RHEA:33655"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:33656"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(9Z,12Z)-octadecadienoate(out) = (9Z,12Z)-octadecadienoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45264"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:45265"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>butanoate(out) = butanoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45248"/>
            <dbReference type="ChEBI" id="CHEBI:17968"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:45249"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>hexadecanoate(out) = hexadecanoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45256"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:45257"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>tetracosanoate(out) = tetracosanoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45260"/>
            <dbReference type="ChEBI" id="CHEBI:31014"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:45261"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>tetradecanoate(out) = tetradecanoate(in)</text>
            <dbReference type="Rhea" id="RHEA:45252"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:45253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane raft</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the CD36 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MET2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019721"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019967.1">
        <property type="protein sequence ID" value="ENSXCOP00000019721.1"/>
        <property type="gene ID" value="ENSXCOG00000014818.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153372"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030169">
        <property type="term" value="F:low-density lipoprotein particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005041">
        <property type="term" value="F:low-density lipoprotein particle receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150094">
        <property type="term" value="P:amyloid-beta clearance by cellular catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044539">
        <property type="term" value="P:long-chain fatty acid import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034383">
        <property type="term" value="P:low-density lipoprotein particle clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005428">
        <property type="entry name" value="CD36/SCARB1/SNMP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002159">
        <property type="entry name" value="CD36_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923:SF12">
        <property type="entry name" value="PLATELET GLYCOPROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11923">
        <property type="entry name" value="SCAVENGER RECEPTOR CLASS B TYPE-1 SR-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01130">
        <property type="entry name" value="CD36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01610">
        <property type="entry name" value="CD36ANTIGEN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01609">
        <property type="entry name" value="CD36FAMILY"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="23 32" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="25" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="19" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="20" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="26" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="21 33" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-0564">Palmitate</keyword>
    <keyword evidence="24" id="KW-0675">Receptor</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15 33" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="18 33" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0813">Transport</keyword>
    <keyword evidence="16" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="33">
        <location>
            <begin position="439"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="32">
        <location>
            <begin position="235"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="32">
        <location>
            <begin position="264"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="32">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00000934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00000996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00010532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00020772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00029966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00031821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00032188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00032780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="PIRSR" id="PIRSR605428-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="468" mass="52109" checksum="BAA0B48437FD2550" modified="2018-12-05" version="1">MGCCNRRCGLIAGAVVGALVAILGGILFPVGDIIIQGTVETEAVIEPGTTAYENWVAAGAKVYRQFWLFDVMNPLEVLEYGATPAVVERGPYTYMTRYLARDNITFHLNHTVTFLLPNGAIFEPSMSVGTEDDKITSLNLAVAGAYSAIPTELHSIVETLIKTTNSSLFQRRTVKELLFGYTDPILRGDVGFFLDYNQTYDGPYDVFTGKDDISKVGKIDMWKGSSLGFWNDEYCDKLNGTDASSFPPFVNKKEPLFFFSSDICRSVSASYESTMNLKGIDVYRYSLHPSTLASPLDNPDNKCFCSNMKTTKNCTLAGVLDISTCQQGRPVYISLPHFLHGSPSLRTDVIGLNPHEEHHKTFLDVEPTTGFTLNFAKRIQVNMMYGPSKVITVLKKVKDYTIFPLVWLNETATLDDETAEMFKKELISRIQMLEIIQQVLLGVGIGVFILCLLGYCIVRRSHNKNEFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEV9</accession>
    <name>A0A3B5MEV9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin family domain containing 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEV9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022105"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022374.1">
        <property type="protein sequence ID" value="ENSXCOP00000022105.1"/>
        <property type="gene ID" value="ENSXCOG00000016520.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012857"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17047">
        <property type="entry name" value="Ubl_UBFD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039120">
        <property type="entry name" value="UBFD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057455">
        <property type="entry name" value="UBFD1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16470">
        <property type="entry name" value="UBIQUITIN DOMAIN-CONTAINING PROTEIN UBFD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16470:SF0">
        <property type="entry name" value="UBIQUITIN DOMAIN-CONTAINING PROTEIN UBFD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25343">
        <property type="entry name" value="PH_UBFD1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="53"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="278" mass="31073" checksum="6AE4B0182EC85D33" modified="2018-12-05" version="1">MDTETEAKPTEAEALGENVEKADAESTSLTDAGNATTQDSSISNGDDADDKQETVDLKIIWNKNKYDLKIPVDSTGAKLKERIHALTGLPPAMQKVMYKGLLPEDKTLRDIKVTNGAKIMVVGSTINDVLAVNTPKEVIQQEVKAEENKKEPLCRQKQHRKVLDKGKPEDIMPSIKGTKERLPTVPLSGMFNKSGGKVRLTFKLEQDQLWIGTKERTEKVPMGSIKNVVSEPIEGHEDYHMMAFQLGPTEASQYWVYWVPAQFVDAIKDTVLGKWQYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHZ3</accession>
    <name>A0A3B5MHZ3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">All-trans-retinol 13,14-reductase</fullName>
            <ecNumber evidence="15">1.3.99.23</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>all-trans-13,14-dihydroretinol + A = all-trans-retinol + AH2</text>
            <dbReference type="Rhea" id="RHEA:19193"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:52075"/>
            <dbReference type="EC" id="1.3.99.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the carotenoid/retinoid oxidoreductase family. CrtISO subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.23" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHZ3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023367"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023648.1">
        <property type="protein sequence ID" value="ENSXCOP00000023367.1"/>
        <property type="gene ID" value="ENSXCOG00000017452.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017613"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051786">
        <property type="term" value="F:all-trans-retinol 13,14-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052206">
        <property type="entry name" value="Retinol_saturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46091:SF1">
        <property type="entry name" value="ALL-TRANS-RETINOL 13,14-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46091">
        <property type="entry name" value="BLR7054 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="509" mass="57463" checksum="5C1C75E3343E48CC" modified="2025-10-08" version="2">MRALIIIYHFMKSDSTLGIHYIGDLLGDSPFRCMLDQMTNGQLQWEPLEKAFDHVVLGSLENRRSYPIFSGSARYLEELKKRFPGEEKAIDEYLRLVKVESYHFGSWLFVVLKLCPLPLAKFLIHTGLVKHLSFFFKMAPRSLRDVVNELTENKDLRAVFTYIFGTYGTPYASFAMHSLLITHYLNGAWYPKAGSSEIAYHMIPIIEKAGGAVLVRAPVNRILFNEAKEACGVSVLKGQEEVHIKAPMVISDAGIFNTYENLLPKELQIQKQLSLVKHGEGGLSILIGLTGTKEELGLKANNYWIFTENNFDELMERYLNDGREQASKKIPLLFVASPSAKDPSWEERSPGKSTLSLVSFVKYSWFEEWKDGKVTNRGSDYKELKQAFIDSALDVVMDVFPKITRDKIEYIDAGTPITNTHYIGAPKGEFYGVDHGIARFNPELNATIRPQTPLKNLYLTGQDVFLCGFAGAIAGALTCGSVILNRNLHLDSFLLAMKTKYIDKKLKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIA8</accession>
    <name>A0A3B5MIA8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Metal transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Metal transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ACDP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIA8"/>
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    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="169"/>
            <end position="188"/>
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        <location>
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            <end position="251"/>
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            <end position="279"/>
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            <begin position="291"/>
            <end position="313"/>
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    </feature>
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            <begin position="167"/>
            <end position="349"/>
        </location>
    </feature>
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        <source>
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            <dbReference type="Ensembl" id="ENSXCOP00000023482.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="516" mass="57175" checksum="3BCCF5D938F8BC32" modified="2018-12-05" version="1">CKGSPQKVPINTSNLAGLRFLLMILLFCGIRFGACSQEAPQVVGLRLEDPGGLVRMKDRIISAPEGATFQLRLFGVNLNGSWPWPWLAFAGAAAGAAGAVGDADPCEEESSRQEAAFNATEPFKADEPNSGLLTVEVKKRGSVSADFYHLCVQSGGMWASVGPDRLQVFPPWGLALLVVLMLLTCGLLRTVNLSLLWLDPVELYVLHSCGSEEDKRAAKRLEPIRRRGNFLTCSLLFLCVLGHSALGVLLYRALGSVVSAVFTSGLLIFFLAELVPHVLCSGYGFQLAPGLTWLAQVCMVLTCPLSCPLGLILDLGLGRDISTCGIRERAMEMIRASVNDPYSEFVKEEFSRGMLRTKTVEDILTPLKDCFMLPSSAVLDFSTMSEIMQSGYTRVPIYEEERSNIVEILYVKDLALVDPEDCTPMTTITKFYNHPLHYVFNDTKLDAMLEEFKKVDLLQSYSHNHVQKLSSCHGDKGTPNVSLCWEWSTRFCELWIFKNNIKKMLQLQTRNSLGFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJA5</accession>
    <name>A0A3B5MJA5_9TELE</name>
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        <recommendedName>
            <fullName evidence="3">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
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            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="CHROMOBOX PROTEIN HOMOLOG 2"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CHROMO_2"/>
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    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="12"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <end position="185"/>
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            <end position="266"/>
        </location>
    </feature>
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        <location>
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            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="78"/>
            <end position="102"/>
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            <begin position="221"/>
            <end position="247"/>
        </location>
    </feature>
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        <location>
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            <end position="300"/>
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            <end position="347"/>
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    </feature>
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        <location>
            <begin position="370"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="480" mass="51254" checksum="92009ABDFB0A25CB" modified="2018-12-05" version="1">MEELSAVGEQVFDAECILNKRLRKGKLEFLVKWRGWSSKEQEKELLMLKRRKRPRGRPRKIIVSIDHSRTNIPEPQKSSTSSSASSSSSSDSSSSCSSSSSSSEDEDEGDDGHAKQASPTVRPRELHPVPQKKAQIVMAKQEPLRKRNRKPLPPEAKEFQQNKGPRKVLKTARETDLPGAIKKPVHPASFTFMGFHRSSAKDAAPGPYRSPLTQGGAVKPATSSPGSGRSSVQTASLALNKSNQIRNATEGKLSISGGNSGGSLDLKTAACKSKGVAALNVNTTKHPVQGTTQHTLSSPSGPKKPHSTGSAMQRVAGTKAGASPSKSSSSNQGHQPLNLQNKQSQSGDAPGTTAASGLRNPVIPARKPTVAQNLETNANKSRLDKSCSEIQNPPARTATKGSKKAKMTDMSTGEEESSSDSDHDSSYAGQGHPVAGQNQDWKPTRSLIEHVFVTDVTANLVTVTVKESPTSVGFFSIRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKA6</accession>
    <name>A0A3B5MKA6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the EGR C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKA6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024809"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025110.1">
        <property type="protein sequence ID" value="ENSXCOP00000024809.1"/>
        <property type="gene ID" value="ENSXCOG00000018526.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165075"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048568">
        <property type="term" value="P:embryonic organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048880">
        <property type="term" value="P:sensory system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000324">
        <property type="entry name" value="Early growth response protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000419">
        <property type="entry name" value="Early growth response protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021849">
        <property type="entry name" value="EGR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF42">
        <property type="entry name" value="EARLY GROWTH RESPONSE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11928">
        <property type="entry name" value="DUF3446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="14 17" id="KW-0804">Transcription</keyword>
    <keyword evidence="10 17" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9 17" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 16 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="228"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="258"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="286"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="303"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="373" mass="39808" checksum="9D988603D7E2AD63" modified="2018-12-05" version="1">MAATKAELLLSALQISEPLFGHPAPPSPLDGYPKLEELQMLLHNAASAAEAAGLLGSEPSEFTDSLCDLPDLQSLPPLTPRLAYSGRFSFEPSTSCATAASSQWAEPLLSLLSGLVSMAAPSSSCSLATSSSSVTSSVTSPSASQSCGSADVTSIFSVTPPAPIPMLPDYLLPQPDAEVDQKPVLPPLTPLPTIKAFSSQFQPQGAACRSRKLPAGRQCKTPPHERPYACPADGCDRRFSRSDELTRHVRVHTGQKPFQCRICMRNFSRSDHLTTHIRTHTGEKPFACAECGRKFARSDERKRHAKIHQRQRDRRADCSAAATPCTSVATPSPPSLPQVYASSLSPHLYSSSCSSPMGSSLSELPSPHSSNIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKW9</accession>
    <name>A0A3B5MKW9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aspartyl/asparaginy/proline hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartyl/asparaginyl beta-hydroxylase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKW9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024397"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024691.1">
        <property type="protein sequence ID" value="ENSXCOP00000024397.1"/>
        <property type="gene ID" value="ENSXCOG00000018218.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00980000198701"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007803">
        <property type="entry name" value="Asp/Arg/Pro-Hydrxlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051821">
        <property type="entry name" value="Asp/Asn_beta-hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46332:SF4">
        <property type="entry name" value="ASPARTATE BETA-HYDROXYLASE DOMAIN-CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46332">
        <property type="entry name" value="ASPARTATE BETA-HYDROXYLASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05118">
        <property type="entry name" value="Asp_Arg_Hydrox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartyl/asparaginy/proline hydroxylase" evidence="5">
        <location>
            <begin position="188"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="357" mass="39521" checksum="EA52EB794C1B74DE" modified="2018-12-05" version="1">MDWSINTLPLPEYVELGVHSLSGLLWTLLFLFLWHCYRIGSDLPIPGHAGKLKSSSRRSMISRSGVCMGTKCSARSKLSRSDQIAPFISMETAKDEEQGQGYLTPVLSHALFPAQASAEAKKLYAALQEYAKRYSWVGMGRIHKGLREQVRLNDLSTIQKPHLFFLPDVPSVPFFPHDAHRHDIEVLEANYPAILAEFQAVYQRGIDSKSGWTCLGPKSQAVFSLYSAGVCVAENCRSCPSTYRTLLSLRTFINSNSLGSAGFWLLGPGAILGSSYGPTNTRLRCHLLQTPTSCELVVGGEPQCWSEGHCLLLDDSFLHTISHKPPESGPRVILSVDLWHPNVAAAERQALDYMFTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLR6</accession>
    <name>A0A3B5MLR6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLR6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022086"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022355.1">
        <property type="protein sequence ID" value="ENSXCOP00000022086.1"/>
        <property type="gene ID" value="ENSXCOG00000016507.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164755"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316:SF41">
        <property type="entry name" value="MAGI FAMILY MEMBER, X-LINKED A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316">
        <property type="entry name" value="MEMBRANE ASSOCIATED GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="13959" checksum="86FBD146E8899A96" modified="2018-12-05" version="1">MSKATVKKLHWRSKPPDFLFLSSGGADYGEFPFVTAAPGGGATVGDIILEIGGTPVLGMTLGDVRGVLNSCPHPIRIKTVFPGSSLCKDLRLYLSKCFTPGSMDSQLQQLIRENLYLRAVPCKTHLDTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNY6</accession>
    <name>A0A3B5MNY6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial</fullName>
            <shortName evidence="1">Glu-AdT subunit C</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">GATC</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (GATB) and C (GATC) subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNY6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022871"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023147.1">
        <property type="protein sequence ID" value="ENSXCOP00000022871.1"/>
        <property type="gene ID" value="ENSXCOG00000017087.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018351"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00122">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="GatC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00135">
        <property type="entry name" value="gatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004:SF0">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02686">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="Glu-tRNAGln amidotransferase C subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="137" mass="15453" checksum="79243865542B2A00" modified="2018-12-05" version="1">MCARCLTTLIVEKSGHTSFHTSLMLQPTKIPADLVDRLERLALVDFRTQQGLECLEKAIRFADQLHVVDTSGVEPMDSVLEDRALYLRDDTVTEGDCAEELLKLAKNTVEEYFVAPPGNIPLPKREERAAILRHSEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNZ7</accession>
    <name>A0A3B5MNZ7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Integrin, alpha 10</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Membrane</location>
            <topology evidence="1 16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNZ7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026139"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026453.1">
        <property type="protein sequence ID" value="ENSXCOP00000026139.1"/>
        <property type="gene ID" value="ENSXCOG00000019521.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158423"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008305">
        <property type="term" value="C:integrin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01469">
        <property type="entry name" value="vWA_integrins_alpha_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048286">
        <property type="entry name" value="Integrin_alpha_Ig-like_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF26">
        <property type="entry name" value="INTEGRIN ALPHA-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20806">
        <property type="entry name" value="Integrin_A_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 16" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 16" id="KW-0401">Integrin</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 16" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1093"/>
            <end position="1117"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="6"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="17">
        <location>
            <begin position="146"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="448"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="512"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="15">
        <location>
            <begin position="574"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1118" mass="124597" checksum="BDB94228FFB36873" modified="2025-10-08" version="2">MCQCFNIDVKHPRVFTGPEDAQFGFSVLQHEADGEKMLVGAPWDGPPNNRKGDVYKCVVGEEKNSNCSKVNLETALRNVSKNLRNSHLGMTLTPDSPDGFLACAPLWSQECGTSMFSTGICASVGDDLEPRGTIAPTEQECQTFMDIVIVVDGSNSIYPWYEVQNFLSNILSKFHISSDQMQVGILQYAEVAVHEWSLKDYQTTQEVVEAAKNISRQEGRETRTAYAIQMATEAFSAERGAREGATKVMIVVTDGESHDGDELPDALEECESRNITRYAIAVLGYYIRKQQDPETFINEIKYIASDPDDEYFFNVTDEAALNDIVDALGDRIFTLETLGYNQSTFHMEMSQIGFSTHTLDDGILFGIVGAYDWDGGVLKEGTNGVIIPPREAFESEFPLELKNHAAYLYTVSSVVVGDWRRLYVAGAPRFKHKGKVVLFELHENGYVDIVQALNGEQIGSYYGSELCGLDIDKDGITDVLLVAAPMYLGSGNKEAGKVYIYTLNGEGKFISNGTLKSDNKSQDARFGYALAAAPDLNHDGFTDLVVGAPLEDEHRGAIYVYHGYDIYIIHDCKQHILGSSLSPSLQYFGRSVSARLDLDGDELIDLAVGAQGRAVLLIVQINVSLSFQPHSINVIQKTCQRGGRESACLNATACFTAVSRSPGSHSNAFGLWVSAMLDDRKFSARALFDESSHRQMQLPVRVQTRKTLCYKLPFHVYDTADYIRPISFSLRFKINDTETGPVLDEGWPTSIPFFKDCGEDDVCTTDLVLQAHLDISGTQKPYVIRSPRRRLSVEVQLQNRLENAYNTSLKLHYSRNLHFSSISIKENANFKIECTALGSNSHSCNVSYPVFRSHSKVNFMLEFEFSCTSLHNRLQMKLNATDSSEREGTLGDNSVQLQTFVQYEPDLFVSESNLNRYEVHPTRTASEAMGPEFYTYIRLLNIGCYSVSNLELRVLLPSVAAEDAAFMTVTDVFTYNATGMKCGVLSDVTQLKSRQKDIRPLHPEDMMHDEILNCSRSWCTEVVCEVRQLGQQAVIRISRRVHDDFFRKVKYKSVRIISAYSLSAQENNLITLGAGTVQGETVLEVLKGQAIPISLWILIGSIIGGLLLLALIIFILWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNZ9</accession>
    <name>A0A3B5MNZ9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FERM and PDZ domain containing 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000025508"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025815.1">
        <property type="protein sequence ID" value="ENSXCOP00000025508.1"/>
        <property type="gene ID" value="ENSXCOG00000019059.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183035"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13183">
        <property type="entry name" value="FERM_C_FRMPD1_FRMPD3_FRMPD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06769">
        <property type="entry name" value="PDZ_FRMPD1_3_4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000203">
        <property type="entry name" value="FERM and PDZ domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000066">
        <property type="entry name" value="FERM and PDZ domain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000053">
        <property type="entry name" value="FERM and PDZ domain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049385">
        <property type="entry name" value="FAK1-like_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041779">
        <property type="entry name" value="FRMPD1/3/4_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46221:SF4">
        <property type="entry name" value="FERM AND PDZ DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46221">
        <property type="entry name" value="FERM AND PDZ DOMAIN-CONTAINING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21477">
        <property type="entry name" value="FERM_C_FAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="3">
        <location>
            <begin position="25"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="FERM" evidence="2">
        <location>
            <begin position="149"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="803"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="855"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="909"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="979"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1165"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="804"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="814"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="922"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1007"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1044"/>
            <end position="1062"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1185" mass="132292" checksum="79E70501D9D72A0C" modified="2018-12-05" version="1">HVSQSNSLEEVCLDGDKFVPPAPRKVEMRRDPVLGFGFVAGSEKPVVVRSVTPGPSEGKLIPGDEIIMINDEPVSSAPRERVIDLVSCKESIVLTVVQPYPSPKSAFISAAKKAKLKTNPVKVRFAEEVIVNGQVPETVKDNSLLFMPNVLKVYLENGQTKSFRFDSNTSIKDVILTLQEKLSIKCIEHFSLMLEQRAEGSASKLMLLHEQEMLTQVTQRPGSHKMKCFFRITFVPKDPVDLLRRDAVAFEYLYVQSCNDVVLERFGSELKYDTALRLAALQMYILTINTKQSQKVSLKYIKEWGLALFLPPAVLSSMKEKNIKKALTHILKTNQNLVPPGKKLTALQAKVHYLKYLSDLRLYGGRVFKSTLVQGEKHTEVTLLVGPKYGISHVINTKTNLVALLADFSHVNRIEMYAEDDNRVRVDLHVLDVKPITLLMESVDAMNLACLTAGYYRLLVDSRRSIFNVAKNCEDRSNQRCSQDYLDQECEYLDHSRCEVQPVYVTEIHQPQHSVHMAERAECCRVPCSQTYLNIPRPKPPDSSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVQISRTFSPESSDSGNETNSSEMTESSELAAAQRHSENHLRMHVAMTEGYRAMNEEKAEVCTTSDENPEEAKSSAVTSSQIFHSDGGEMEPDTMEIKSVSEFFTKMHMGSLMSRQRGRQREAESSIQGDRCSSSEKFNSTSLDSAKEEPPHLVGKYNTFTVRDSYYMNQLDLGRTHCKDRHQKWQQRAPGSKMAENLASECANDSQASHADRLTAKGENQDSDERNQQLNAHLISPSKGPFPAESDATSQDNKQKQIKFSQSEQDVTRMYEYHVSKRMSSIQSEGVHSLQSSQCSSIDAGCSTGSSSWVTPMDSPLCATDSMHVLSESSLKGMSYAAGAEEKAYGPTVQGKVGHTMDPTLMRKIHAATSAEPGFGSSHKMPKIKETT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPH2</accession>
    <name>A0A3B5MPH2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta/gamma crystallin 'Greek key' domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MPH2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023348.1">
        <property type="protein sequence ID" value="ENSXCOP00000023071.1"/>
        <property type="gene ID" value="ENSXCOG00000017236.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163148"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000001">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF139">
        <property type="entry name" value="CRYSTALLIN, GAMMA M1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="11"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="50"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="138"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="183" mass="22166" checksum="89F15E4C7C04DB1B" modified="2018-12-05" version="1">HRSSSMRTETSRIIFYEERNFQGRSYETSSDCADMSSHLSRCHSCRVESGCFMVYDRTNFMGNQYFVRRGEYSDYQRMGMSDCIRSCRMIPMHRGQFRIRIYEREDFGGQMHECMEDCDSIMDRYRMSDCMSCHVMDGHWLMYEQPQFRGRMMYLRPGEYRSFREMGYGGMRFMSMRRIMDSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRZ2</accession>
    <name>A0A3B5MRZ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Putative nuclease HARBI1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Harbinger transposase-derived nuclease</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Transposase-derived protein that may have nuclease activity. Does not have transposase activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRZ2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027179"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027512.1">
        <property type="protein sequence ID" value="ENSXCOP00000027179.1"/>
        <property type="gene ID" value="ENSXCOG00000020296.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154348"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026103">
        <property type="entry name" value="HARBI1_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF253">
        <property type="entry name" value="NUCLEASE HARBI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02086">
        <property type="entry name" value="PUTNUCHARBI1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="13">
        <location>
            <begin position="133"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="326" mass="36482" checksum="43DC0259B0DC720D" modified="2018-12-05" version="1">MAIPIAILDCDLLLHGRGHKTLERFDLDTVSNSVLLSNFGFPRDFILYLVELLRETLSRRTLRSRAISPEVQVLAALGFYTSGSFQTSMGDIIGISQASMSRCVTNVTKALVEKAPQFITFNKRKFVLILPQQVAIKAPNSDASSYVNKKGFHSIACQLVCNARGLLLSAETWPGGLQDTKILQRSAISKQLQDNEEGWLLGDGRYPLKKWLMTPVESPGTAAEFQYNLAHTVTHEIVDRTFRAIQTRFRCLDGIKGNLQYSPERSSSILLACCVLHNASLHSGLDAWTLERTEPLEQPKGPEQTLEDRDSEAEELRKHIILKHFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MU78</accession>
    <name>A0A3B5MU78_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transmembrane protein 266</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MU78"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027051"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027384.1">
        <property type="protein sequence ID" value="ENSXCOP00000027051.1"/>
        <property type="gene ID" value="ENSXCOG00000020205.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156738"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005216">
        <property type="term" value="F:monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022832">
        <property type="term" value="F:voltage-gated channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042857">
        <property type="entry name" value="TMEM266"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46842">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46842:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="8">
        <location>
            <begin position="71"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="321"/>
            <end position="415"/>
        </location>
    </feature>
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        <location>
            <begin position="436"/>
            <end position="458"/>
        </location>
    </feature>
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        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
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        <location>
            <begin position="321"/>
            <end position="331"/>
        </location>
    </feature>
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        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
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        <location>
            <begin position="361"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
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            <dbReference type="Ensembl" id="ENSXCOP00000027051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="511" mass="56372" checksum="D1908E96D8CEDE5C" modified="2018-12-05" version="1">VEDDNQCLAPVQLVNFTYRDLPLAALDISLAGSQLISNPDEEDNRDGSNWLKPCCGRRAALWQVCLLSAGFNCFLVACVILVVLLLTVELLIDIKLLQFNNASQFASIIHWISLAILSVFFTETVFRIVVLGIWDYIENKVEVFDGAVIVLSLAPMVASTVANGPSSPWDAIGLIISLRIWRVKRIIDAYVLQVKVEMELEIQQYEKAKAVREEQLDRLTQICQEQAFEIRQLRAHLAQQDLDLVAEREAAMQIHHMWGKESSSFQEVDGLAAGASDNHRPAKDDMNNYISQYYSEPSSDMGIPDPARVITTAAIDVHLPNNPSQLPSTLVSADAAPSSRLQRTSSSVSEASTTAVSRSSFSARQHSISSHTLGSSMDCSSTVREASTSTDYSDQRCYPPPYSSPLALGPQPRGSPSAVVQELLYSLSEDSCLTQKGLDPVNLKPPSPTGSTKTSPELERRLNIYNKRNQESRIGLHGKPVIHVQGNEPLLEEKYRMMGSVETSVNRLSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWA5</accession>
    <name>A0A3B5MWA5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Overexpressed in colon carcinoma 1 protein homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWA5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028287.1">
        <property type="protein sequence ID" value="ENSXCOP00000027951.1"/>
        <property type="gene ID" value="ENSXCOG00000020845.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177234"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029133">
        <property type="entry name" value="OCC1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15506">
        <property type="entry name" value="OCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="59" mass="6045" checksum="39D6457594DBBA92" modified="2018-12-05" version="1">MGCGNSSATSTSGGGECFRNLCVDVNYGGVYVGLPADLTTVAASQSKSTRKGEHLLQTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZS0</accession>
    <name>A0A3B5MZS0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">DNA-directed RNA polymerase III subunit RPC3</fullName>
            <shortName evidence="7">RNA polymerase III subunit C3</shortName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the eukaryotic RPC3/POLR3C RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MZS0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026140"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026454.1">
        <property type="protein sequence ID" value="ENSXCOP00000026140.1"/>
        <property type="gene ID" value="ENSXCOG00000019524.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002799"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase III subunit RPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000218">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit RPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000256">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit RPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000262">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit RPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055207">
        <property type="entry name" value="POLR3C_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013197">
        <property type="entry name" value="RNA_pol_III_RPC82-rel_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008806">
        <property type="entry name" value="RNA_pol_III_Rpc82_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039748">
        <property type="entry name" value="RPC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949">
        <property type="entry name" value="RNA POLYMERASE III DNA DIRECTED -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08221">
        <property type="entry name" value="HTH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05645">
        <property type="entry name" value="RNA_pol_Rpc82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20912">
        <property type="entry name" value="RPC3_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22536">
        <property type="entry name" value="WHD_POLR3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase III subunit RPC82-related helix-turn-helix" evidence="10">
        <location>
            <begin position="8"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase III Rpc82 C -terminal" evidence="9">
        <location>
            <begin position="147"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase III subunit RPC3 winged-helix" evidence="11">
        <location>
            <begin position="342"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="195"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="205"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="509" mass="57022" checksum="CA1197F0E5A88039" modified="2018-12-05" version="1">MTAQEIRLCGLLLQEHFGEVVEKVGTHLLRRGAQNLRTIVQETNLSVKKCLCVLVQHGACCFTSGRKGPGSPTEYRACSDRILRVLRYPRYIYTAKTLYGDTGELIIEELLQRGHMTMSGTVKTVADRLTQNMEEGHSMDYSEVSSAFSKLVETHFLQRCPPVGGAGTSGTTAPAAAPSFPECYSVPHVSLIGRGKRQLVPENGEDQRNAKRAKMDTETHGDEGIHWQVNFERFHQHFRDQAIISAVANKLDQTSSEIVRTILRMSEVTTSATAACTKPLSANEVFRSLPASYNIPRHILDQYLTLLVDDPMEFVGKTGESGGGMFVVNLHRALANLARATVESIVQERFGSRSARIFRLLLRKRHLEQKQVEDFAMIPAKEAKDMLYTLLSHNLVQLQVNHNSLIDKMLLKCPFWVTVANLIERRLFETKENKRLLEKSQRIEAILASLQASGAEPEQLSEVEEMISAPEKQQLDVLKLHVNKLDSAENQVDETIFILESYVSSTSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N1B2</accession>
    <name>A0A3B5N1B2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N1B2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027375"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027708.1">
        <property type="protein sequence ID" value="ENSXCOP00000027375.1"/>
        <property type="gene ID" value="ENSXCOG00000020442.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153678"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17677">
        <property type="entry name" value="RUN1_DENND5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047278">
        <property type="entry name" value="DEN5A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46070:SF3">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46070">
        <property type="entry name" value="PINSTRIPE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03455">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00801">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00593">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00800">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50826">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="UDENN" evidence="6">
        <location>
            <begin position="34"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="RUN" evidence="7">
        <location>
            <begin position="773"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="domain" description="RUN" evidence="7">
        <location>
            <begin position="1112"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="135"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="674"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="141"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="684"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1268" mass="140910" checksum="DA374AEE6556AC23" modified="2018-12-05" version="1">MNGTAAATGGSASCRFAHYFVVCGVDTETGLEPDDGAGESFEQSPIRRSFKSKVLVHYPENMDRNPFNKDAVNMLCLPRGLSFCTQADSLDPQFHSFTVTSDDGTRSYGFVHTFYEEVTSPQIITAMQTLYQMHHVEHHSSSSTSSPSSSTSSSASTPSTSSMDSLASSLEESDAESLTSGVSGCLGCVGSFDAARDTLYVSKALCLITPLPFLQASRQFLAQLHQAVTSQMAPPLPLESYVYNILYEVPLPPPGRSLRFHGVQGPIVCQRPGPGELPLGDYPLGEAFSLLGVDNMVKLLTCALLETQVLLYSKDYQRLMMVAEGITTLLFPFQWQHIYLPIISTPLHHLLDAPVPFLMGIQHRNGAQRSSLHLPHEANLCFVDIDSHCVEAPEDLPQFPDQTELIQELSEVLLSFGLPPRGGVTATTTTGSTSSSARLSSLVLEDLMEDRRNGNLGGEELAVLERLQALARRCGGGKVSEGGKTLGHVFEEEEEELKAAKLNIQLREVFAVRFAAIFGRYEDFIVNSAMDLDSWLNNRDGTCSFDKGSFLSVQPAAHLHFLSRFLETSMFSSFVDGKVVSRWVDREPLQQLFDGRMERERLYDISDEESRNRPYRKCTTLFESAQAIERRLLKTDHTAIHPHLLDMKIGQGRHHPGYFPRLQADVLAQAHNTHKYSSRITASRRTEPRRSVADHSGTDSEPKQVLTHTATHTGLKSCIIYLQGHFLCSIETRIHKESLQTDETPRPTKRMLKERMGKECVELGQGEANITGLQENTLIHGLCDLLERTWGHGLQIKQGKSAIWSHLLHYQAAQEKMEAPADVPGSNSLDQRLSDDGPLLLRGSLIQDMRFIQTMRDDLSEVGQARAWIHLALEKKMLSQHLKELLTNPELLRQLYKPHAFLLCEEEREQFLYHLLSLNTVDYLCFTRTFTSISIPYRVVIIPMKKLSIAMATVNPWVCVSGELGDSGVRQIPKNAQEIFFQNLGRLSTLQLGVQENSGLLAKCLIDCVVVYNEITGHTYRFPCGRWLGRGVGDGSLERVLIGQLVSPGAEEDAGRWSGTPPELASPSQSVRAVLGSLGGMHSAEVQEDLREAANNLVKHFHKPEQERGNLTVLLCGEGGLVLALEKFLLHGFKSNRLFQRSVFVWDFVEKAVVSMETNDQMGDLHGSTLPKSPPCDLLCRYVHAINSSPRNIGKEGKFQLFVCLGIRDKLLSQWFPLLAECPLTARTYEDGALLRDRAALHSLCRILQTLNDFSITLEAALVKGVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N1H6</accession>
    <name>A0A3B5N1H6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glucagon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">GCG</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glucagon family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N1H6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027510"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027844.1">
        <property type="protein sequence ID" value="ENSXCOP00000027510.1"/>
        <property type="gene ID" value="ENSXCOG00000020536.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005372"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031769">
        <property type="term" value="F:glucagon receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.590">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015550">
        <property type="entry name" value="Glucagon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000532">
        <property type="entry name" value="Glucagon_GIP_secretin_VIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11418">
        <property type="entry name" value="GLUCAGON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11418:SF0">
        <property type="entry name" value="PRO-GLUCAGON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00123">
        <property type="entry name" value="Hormone_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00275">
        <property type="entry name" value="GLUCAGON"/>
    </dbReference>
    <dbReference type="SMART" id="SM00070">
        <property type="entry name" value="GLUCA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00260">
        <property type="entry name" value="GLUCAGON"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0372">Hormone</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017338515" evidence="5">
        <location>
            <begin position="23"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Glucagon / GIP / secretin / VIP family" evidence="6">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Glucagon / GIP / secretin / VIP family" evidence="6">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="180" mass="20330" checksum="0C1BD2BB9A82F350" modified="2018-12-05" version="1">MRSIHCLAGILLVLGFMQVSWQVPLLGTEDNSSFEEDNRLASGPQELSNIKRHSEGTFSNDYSKYLEDRKAQDFVRWLMSNKRSGLVADKRHADGTFTSDVSSYLKDQAIKDFVAQLKSGQIQKESEMDSLAQTISRRHVDGSFTSDVNKVLDSMAAKEYLLWVMTSKPSGESNKRQDNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KJ33</accession>
    <name>A0A3B5KJ33_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <property type="protein sequence ID" value="ENSXCOP00000000108.1"/>
        <property type="gene ID" value="ENSXCOG00000000086.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Znf_C2H2C"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="82" mass="9426" checksum="2D78A2AA9082FD3E" modified="2018-12-05" version="1">NEFRGELVNQRWTRGERNSSRCQASQRLQSRRPIMMSVEGDDKRTRTRSKGIRPIELVGQELCPTPGCNGSGHISGRYSRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KJD6</accession>
    <name>A0A3B5KJD6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Solute carrier family 7 member 14a</fullName>
        </submittedName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:amino acid transmembrane transporter activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:eye development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CAT_C"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AA_permease_C"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="2">
        <location>
            <begin position="229"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="378" mass="42027" checksum="776AB38653553F93" modified="2025-10-08" version="2">MHPLHNNVLLCVCLRHKIPMKTTEVSLCVLLLRYQPEGDIHGFVNFLSEEQNKKKKEGILAECEKDACSPTSEADEYGGQTTNTCGAKNLPSLGDNEMLIGKPDKNAYSAGHPNYGTVDMTTGIEAEESEGGMSRRLKKLIGPRYYTLRIRLGLPGKMDRPTPVTGKTVTVCVLLLFILFFAFCSFIIFGKSKSGHWWALLLLIFLIIFIALLIIIILQQPENPKRLPYMAPCVPFVPASAMLVNIYLMLKLSAITWIRFSIWCLVGVLIYFGYGMWNSTLEITARENEVHASTYQRYDQGVDEGFCGFDDDFYPPSTDAWGAPGAGLEATPPPKANSFIFHLPSCSPLDINRATSAESNLSESRVIGHQKLACTLEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KLM2</accession>
    <name>A0A3B5KLM2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Suppressor of cytokine signaling 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
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            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Protein modification; protein ubiquitination.</text>
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        <property type="nucleotide sequence ID" value="XM_028015847.1"/>
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        <property type="protein sequence ID" value="ENSXCOP00000001008.1"/>
        <property type="gene ID" value="ENSXCOG00000000827.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="xco:114143623"/>
    <dbReference type="CTD" id="122809"/>
    <dbReference type="GeneTree" id="ENSGT00940000166202"/>
    <dbReference type="UniPathway" id="UPA00143"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="P:protein ubiquitination"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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        <property type="entry name" value="SOCS4/SOCS5_dom"/>
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        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
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    </dbReference>
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        <property type="entry name" value="SUPPRESSOR OF CYTOKINE SIGNALING 4 ISOFORM X1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="3" id="KW-0734">Signal transduction inhibitor</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SH2" evidence="8">
        <location>
            <begin position="242"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="9">
        <location>
            <begin position="332"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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            <dbReference type="ARBA" id="ARBA00022786"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022999"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50001"/>
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            <dbReference type="PROSITE" id="PS50225"/>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001008.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="400" mass="45264" checksum="9DC024A6C050DB17" modified="2018-12-05" version="1">MSEKTPRGADTRPKSGLRSWSADSYIWRGKKRSRSSRNGSSPGGSEADGSEELGMRSTSCSRRRRERKCSCSALGESLMAGDMDGVCRKALHRRSLRQKFQDAVGQCLPLRHHHHHHYHHPPGSSRPFSVLFWSKRKIHVSELMQDKCPFSPKSELAKCWHLIKNQVGHPGSLKDMETPLKPRVSSSTSPPQTPLSWEDICCSPAPLEDWDPSSLHGRPDGACANTDYILVPDLLQINNNPCYWGVLNRFEAEELLEGQPEGTFLLRDSAQDEFLFSVSFRRYSRSLHARIEQNGKRFSFDVRDPCMYRDASVTGLLRHYSDPATCLFFEPLLSRPLPRTFPFPLQHLCRAVICSCTTYQGVEGLPLPPQLREYLRQYHIKCDGACAVSMPTAANTQDLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KS66</accession>
    <name>A0A3B5KS66_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Solute carrier family 40 member</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in iron transport and iron homeostasis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ferroportin (FP) (TC 2.A.100) family. SLC40A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KS66"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000476"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000484.1">
        <property type="protein sequence ID" value="ENSXCOP00000000476.1"/>
        <property type="gene ID" value="ENSXCOG00000000413.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164650"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd17480">
        <property type="entry name" value="MFS_SLC40A1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009716">
        <property type="entry name" value="Ferroportin-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11660">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 40 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11660:SF50">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 40 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06963">
        <property type="entry name" value="FPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="18"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="425"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="470"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="493"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="526" mass="58256" checksum="66666585011BD902" modified="2018-12-05" version="1">MWHFAISVFLIELYGRNLLLTAVFGLVLAGSVLLLGALIGDWVDRNPRNKVAHASLFIQNTSVTVCSIVLMLVFSYKEQIELIWDGWFTVVCYTVVIILADVANLASTALTIAIQRDWIVVITGYNRGHLAGMNATMRRIDQVTNILAPLAVGQVMTLASNVVGCGFILAWNLFSLIVEFFFLSRVYRIVPALSVKPPTVEGDQEYLARTERRGLQGTGVFSQLILMKKKNYMHLKEITNLPLCFQRFRWLLRTCMDGWRAYYRQPVFLAGLGLAFLYTTVLGFDCITTGYAYTQGISGSLLSLLMGVSAITGLMGTVMFTRLRKSVGLVNTGIISSCLHLGCLLLCVCSVFAPGSPIDLSLLMPYFSSGSSAEPGGMASKRQKHTFPLSGGSNQPLLPDRSSIHWTNNTVLFDNVPSGTAPDSYISIILLFLGVITARIGLWCFDLTVTQLLQETICESERGVVNGVQSSMNYLMDLLHFIMVISAPQPQHFGILVIISVIFITTGHSMYFLYAHRAKRKHRLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTC3</accession>
    <name>A0A3B5KTC3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts with ODC1 and thereby sterically blocks ODC homodimerization.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ODC antizyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTC3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001220"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001238.1">
        <property type="protein sequence ID" value="ENSXCOP00000001220.1"/>
        <property type="gene ID" value="ENSXCOG00000001002.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157725"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008073">
        <property type="term" value="F:ornithine decarboxylase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075523">
        <property type="term" value="P:viral translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.60:FF:000001">
        <property type="entry name" value="Ornithine decarboxylase antizyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002993">
        <property type="entry name" value="ODC_AZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038581">
        <property type="entry name" value="ODC_AZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10279">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE ANTIZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10279:SF6">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE ANTIZYME 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02100">
        <property type="entry name" value="ODC_AZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01337">
        <property type="entry name" value="ODC_AZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0688">Ribosomal frameshifting</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="177" mass="19860" checksum="9150BF2C8E522417" modified="2018-12-05" version="1">SWLSGALKPSSIPQAPGPLWCSDAPHPQLKIPGGRGTVRDHSHAVLIHKQDDRLTVTQATPVGGRPSLLRFHYQLTERRSAFWDTALSGDSLFLEIPAGSLVEGSKELTTLLEFAEEKLKVNYVFLWFHKNREDRVIIKTFHYMGFEMVKPGNPMVPARPDLAFMAYTLDNSSSDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KUK6</accession>
    <name>A0A3B5KUK6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KUK6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002228.1">
        <property type="protein sequence ID" value="ENSXCOP00000002197.1"/>
        <property type="gene ID" value="ENSXCOG00000001752.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182934"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051378">
        <property type="term" value="F:serotonin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071875">
        <property type="term" value="P:adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000523">
        <property type="entry name" value="5-hydroxytryptamine receptor 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248">
        <property type="entry name" value="ADRENERGIC RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24248:SF192">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0085">Behavior</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="50"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="332" mass="37464" checksum="51B14EBEB545010C" modified="2018-12-05" version="1">QDAETSTSSSGSLIKIFTNIQNSAEVDGQTVTSYILNAFVVTWSILIICGNLLVIISVVYFKQLHTPTNYLILSLAVTDLLVGALVLPFSTILSLSSCWNSSYLLCKMRGLFDILLCASSILNLCCISIDRYYAVCHPLTYRSKMNVRVILAMILVSWALSALLAISITGRGLTQGNLNRRCAIFIATKETSPGPFFEFYIPAIIILTIYLRILMVAKKQARRIQNTMKSEAALSKMEKKANITLTVVMGVFLMCWTPFFLSLGLYSIGKFTIPVAVMEAFKWLGWSNSMLNPLVYAFFYRWFRRAFKMIIFEVIIFLILICNNETVIYHIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KV76</accession>
    <name>A0A3B5KV76_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KV76"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001511"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001534.1">
        <property type="protein sequence ID" value="ENSXCOP00000001511.1"/>
        <property type="gene ID" value="ENSXCOG00000001224.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="80" mass="8817" checksum="71E91052A6C1043D" modified="2018-12-05" version="1">WTIGSTGCRRSLFDAGVKGRGLRASRELSAGEVLLAEPGYAAVLIYPPVDFWEPISFILGHRDRLILTREADLNFISKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KVN2</accession>
    <name>A0A3B5KVN2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RNA binding motif, single stranded interacting protein 1a</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KVN2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002100.1">
        <property type="protein sequence ID" value="ENSXCOP00000002070.1"/>
        <property type="gene ID" value="ENSXCOG00000001659.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156082"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="protein alan shepard isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000450">
        <property type="entry name" value="RNA binding motif, single stranded interacting protein 1a"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="43"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="122"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="356" mass="38575" checksum="361BFB9243DF4121" modified="2018-12-05" version="1">MIFAHTGNPLKTSNRKQSYPMTPSSPSSSSNSSSTGLEQLSKTNLYIRGLPPATTDLDLVKLCHQYGKIQSAKAILDKTTNKCKGYGFVDFDSPAAALKAVHALKTSGIQAQMAKQQEQDPTNLYISNLPLSVDERELEKMLQPFGQVVSTRILRDYSGNSRGVGFARMDTTEQCNAVISHFNGKFIKLASGALGPSQPLLCKFADSQRKKHAHSGYVPNGQTGDLRLGAMTLTYDPSTAAIQNGYYPSPYPVTNRIMTVQPAMSPYMSPVSAYQVQSHSWVAHQPYIMQHPAAVMSPSVDPSMSMHPTAMITQQMGQLSLGNTGAVYAALALQENGTLQQVDSSNNSSPYSQLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KVU6</accession>
    <name>A0A3B5KVU6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KVU6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004243"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004290.1">
        <property type="protein sequence ID" value="ENSXCOP00000004243.1"/>
        <property type="gene ID" value="ENSXCOG00000003336.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000271001"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="8044" checksum="804767E79B19FA80" modified="2018-12-05" version="1">MDQNNLARVFGPTIVGHSIAEPSPTTIMKDTTIQPKVCLKELPASGPRQIHCVLPSVLCVPLPAPCVHQVPLHIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXC2</accession>
    <name>A0A3B5KXC2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 19">Cellular tumor antigen p53</fullName>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15 19">Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence. Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Negatively regulates cell division by controlling expression of a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 19">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="16 19">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 19">Binds DNA as a homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 19">Belongs to the p53 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXC2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002276"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002310.1">
        <property type="protein sequence ID" value="ENSXCOP00000002276.1"/>
        <property type="gene ID" value="ENSXCOG00000001823.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183153"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08367">
        <property type="entry name" value="P53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.170.10">
        <property type="entry name" value="p53-like tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012346">
        <property type="entry name" value="p53/RUNT-type_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057064">
        <property type="entry name" value="P53_central_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011615">
        <property type="entry name" value="p53_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036674">
        <property type="entry name" value="p53_tetramer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010991">
        <property type="entry name" value="p53_tetrameristn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013872">
        <property type="entry name" value="p53_transactivation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002117">
        <property type="entry name" value="p53_tumour_suppressor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11447">
        <property type="entry name" value="CELLULAR TUMOR ANTIGEN P53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11447:SF6">
        <property type="entry name" value="CELLULAR TUMOR ANTIGEN P53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00870">
        <property type="entry name" value="P53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08563">
        <property type="entry name" value="P53_TAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07710">
        <property type="entry name" value="P53_tetramer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00386">
        <property type="entry name" value="P53SUPPRESSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47719">
        <property type="entry name" value="p53 tetramerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00348">
        <property type="entry name" value="P53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0010">Activator</keyword>
    <keyword evidence="6 19" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="14 19" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 19" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 16 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 19" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 19" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8 16 19" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="p53 transactivation" evidence="23">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="p53 DNA-binding" evidence="21">
        <location>
            <begin position="58"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="p53 tetramerisation" evidence="22">
        <location>
            <begin position="267"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="234"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="234"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="261"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="17">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)" evidence="18">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR602117-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR602117-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR602117-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF07710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF08563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="332" mass="36883" checksum="5B2F98705B81B097" modified="2018-12-05" version="1">AVTVKARMEEADLTLPLSQDTFHDLWNNSQNMDFWEDPETMQETKNVPTAPTVPAISNYAGEHGFNLEFHDSGTAKSVTSTYSVKLGKLFCQLAKTTPIGVLVKEEPPQGAVIRATAVYKKTEHVGEVVKRCPHHQSEDLSDNKSHLIRVEGSQLAQYFEDPNTRRHSVTVPYERPQLGSEMTTILLSFMCNSSCMGGMNRRPILTILTLETTEGEVLGRRCFEVRVCACPGRDRKTEEGNLEKSGTKQTKKRKSAPAPDTSTAKKSKSASSGEDEDKEIYTLSIRGRNRYLWFKSLNDGLELMDKTGPKIKQEIPAPSSGKRLLKGGSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXF0</accession>
    <name>A0A3B5KXF0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VWFD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXF0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000239"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000245.1">
        <property type="protein sequence ID" value="ENSXCOP00000000239.1"/>
        <property type="gene ID" value="ENSXCOG00000000182.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157490"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050780">
        <property type="entry name" value="Mucin_vWF_Thrombospondin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014853">
        <property type="entry name" value="VWF/SSPO/ZAN-like_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001846">
        <property type="entry name" value="VWF_type-D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339">
        <property type="entry name" value="EXTRACELLULAR MATRIX GLYCOPROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11339:SF228">
        <property type="entry name" value="OTOGELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08742">
        <property type="entry name" value="C8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00094">
        <property type="entry name" value="VWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00832">
        <property type="entry name" value="C8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51233">
        <property type="entry name" value="VWFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="231" mass="25890" checksum="0D65001A4CE5E7BE" modified="2018-12-05" version="1">MQNPSSVIDRKQQMFIWPAGYFTIIHFPEEDVTVLWDRKTTVHVQVGPRWQGKLSGLCGNFDLKTVNEMRTPDNIDSPTPQEFGNSWTAMEVSGCVNSPDIRHPCSLSPLREPFAKRRCAVLLSEVFQTCHPVVDVTWFYTNCLADTCACSRGGDCECFCTSVAAYAGRCCHEGVSVDWRSPSLCRKKTLPLIGLFLPNICLMLELHCKKHKILPTVCGLVSNVSILVHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXG5</accession>
    <name>A0A3B5KXG5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-acylglucosamine 2-epimerase</fullName>
            <ecNumber evidence="3">5.1.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">GlcNAc 2-epimerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">N-acetyl-D-glucosamine 2-epimerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Renin-binding protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>an N-acyl-D-glucosamine = an N-acyl-D-mannosamine</text>
            <dbReference type="Rhea" id="RHEA:19033"/>
            <dbReference type="ChEBI" id="CHEBI:16062"/>
            <dbReference type="ChEBI" id="CHEBI:17274"/>
            <dbReference type="EC" id="5.1.3.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19034"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:19035"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-sugar metabolism; N-acetylneuraminate degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer. Forms a heterodimer with renin and inhibits its activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the N-acylglucosamine 2-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.8" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXG5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002725"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002762.1">
        <property type="protein sequence ID" value="ENSXCOP00000002725.1"/>
        <property type="gene ID" value="ENSXCOG00000002164.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013740"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050121">
        <property type="term" value="F:N-acylglucosamine 2-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010819">
        <property type="entry name" value="AGE/CE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15108">
        <property type="entry name" value="N-ACYLGLUCOSAMINE-2-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07221">
        <property type="entry name" value="GlcNAc_2-epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="272" mass="30689" checksum="5F79666C2BCFAC46" modified="2018-12-05" version="1">CVCVGVCVCVCLEAEQMMEQLIYWVREDASGLGRPQLPGDDPTNSMAVPMMLLCLVQQLSDGRPETGGKYAELGSWCVSEIQQHVQRDGTAILENVSATGAELPGCLGRLQNPGHALEAGWFLLRYAADNDNKEIQRTAIEKFVELPFESGWDKAHGGLFYFLDVDGHCPTQLEWSMKLWWPHSEALVAFLMAYSQTKNPELLENFFRVYEYTFSHFPDSESGEWFGYLTQEGKVALDFKGGPFKGFFHVPRCLYMCESLLDDLMTTIEEPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KY14</accession>
    <name>A0A3B5KY14_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transmembrane protein 100</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
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    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KY14"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002560.1">
        <property type="protein sequence ID" value="ENSXCOP00000002526.1"/>
        <property type="gene ID" value="ENSXCOG00000002020.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154322"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071773">
        <property type="term" value="P:cellular response to BMP stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032536">
        <property type="entry name" value="TMEM100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16100">
        <property type="entry name" value="PHOSPHOINOSITIDE-INTERACTING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16100:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16311">
        <property type="entry name" value="TMEM100"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="17860" checksum="6176D517ABFF3252" modified="2018-12-05" version="1">MALKIIFRDIPAECGVFRVTCQNTAWCQKEKKNVIDKAMSTPHCGSDFTIYTSKWNLGRGQARPPESLHTLERLAQATGGTEKSWYRCVFPFGVISLVIGMAGTGVTFTFNTLPQTKVVSVALLCSGVVMLLVAAVCWRAHRLRRRKKKESGFFTADQGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KY79</accession>
    <name>A0A3B5KY79_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KY79"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002626.1">
        <property type="protein sequence ID" value="ENSXCOP00000002591.1"/>
        <property type="gene ID" value="ENSXCOG00000002075.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064862"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33802">
        <property type="entry name" value="SI:CH211-161H7.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33802:SF4">
        <property type="entry name" value="SI:DKEY-29D8.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="239" mass="27470" checksum="0C252BC129354F14" modified="2018-12-05" version="1">PFHFSTGNVSARYDTGITPAGWTFSIWGVIYTWLTLMVIYVTSYVFRSWAQSLLPYAFYFSWLSNMLLNVTWLMLWDKLMLAALVVLILIAFTNYSTLFFVCFATDYYGLWLQSYHSKDLVCLRILVQNGLAVYATWSSIASLLNFSVVLQLWGVDKSTAATISLCILFAEFVLENWVLDRWVRNILTVYPVVIVALVGIIYRHFNPADPTPNTVFMVQLVIACILFVSRVCSVIWRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1B7</accession>
    <name>A0A3B5L1B7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1B7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003726.1">
        <property type="protein sequence ID" value="ENSXCOP00000003686.1"/>
        <property type="gene ID" value="ENSXCOG00000002892.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000271692"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2" id="KW-0732">Signal</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017430425" evidence="2">
        <location>
            <begin position="25"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="94" mass="10524" checksum="03080FBA2F533F13" modified="2018-12-05" version="1">LGRFQRHMQTHLLVLLCPSGKITATKNSPTTIPNSIHTHTLGLEEITFSLGLVLLHSSSYLIFLLIFKQKSSGKQIILFLIMYAHVFSVLLTGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1E8</accession>
    <name>A0A3B5L1E8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HMG box domain containing 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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            <taxon>Poeciliinae</taxon>
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        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1E8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004160"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004206.1">
        <property type="protein sequence ID" value="ENSXCOP00000004160.1"/>
        <property type="gene ID" value="ENSXCOG00000003266.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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    </dbReference>
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        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039598">
        <property type="entry name" value="HMGXB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040648">
        <property type="entry name" value="HMGXB3_CxC4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17609">
        <property type="entry name" value="HMG DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17609:SF2">
        <property type="entry name" value="HMG DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18717">
        <property type="entry name" value="CxC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09011">
        <property type="entry name" value="HMG_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="3">
        <location>
            <begin position="44"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="1">
        <location>
            <begin position="44"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="369"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="941" mass="104757" checksum="313B6E6624FC3AEB" modified="2018-12-05" version="1">MEDMDNVEVLEVVKVTEEVENVYSQVEMTSPKKKRGKTDENSHERPKKSAYLLYYFDVHQVMQLGTPNLPQSEVNKRISESWRRLSVAEKSYYLERAKLEKEGIDTTSQSSSKHLPGFRKILPRANCFLVPNSASSNYQAVGSQSEVCIESIDFSAEGSLCSVALRVPKSKVAPVDLATEMEISEPPTVPSDTAEETVVPSYSVKTYTRRGRGRCLNPGCSFVYVTRHKPPTCPECGSHLGGKWIPAVKVELCIRCSSDVVVLVATVPQEILSGLKPSTLKQLGQTVPTTTTIQVKTHPSILKTQSDSDEVKFLHSLHPLLQDSSVSDLGLSTARGRGRCKNPACDYMYKNRHKPAVCPKCGTELTRKNTKPPKTLLDPHQDLSPAQRDVQRQSTLQLIRSTLQIPESDTELQETLSLIQELNNVKIVLVKNGEGSDAETETLLETGWPQFYESAATHCGLCSYHLLKGGRGTVAGQEDCWLLTETLMQTASLQLKVCPNLRCLALHSFADLHPGLFNVGNRLLVSLDLFLKIRANIKLGQTPSLAAQSVLTPEESSQVQELFLSGYWAFECLTVRDYNDMICGICGVAPKVEIAQRHRNDVLELKNVEFTWPECSVSDEVHVDDFWLTMEGEAIEQAAFPADIPITRVDASIIAPFAPPLMRSPTVINTEKDKLMPQIQQPAGDPSALVRLIHDGQLRLDRVEDHSEDELRTILDSCGVGLTPGSTKVCVCLGRKQVVCSSKYLVRGETARDHVDLLLSSRYWPPVYVCDCPRQVALCTDLQYPELATQMWGRNQGCFSDPFEKPEVSFVTCPELQDQPYTADLSLVAENQLVHPITKSSSCWLVYPPGAENNVNTKSIIFNNTAYYYLYNRLVDFLSSRDIVNQQINQVVKACQPGEVVIRDSLYRLGVAQINTDEDEGTRLDAQTEEEGEKQLMVLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1W4</accession>
    <name>A0A3B5L1W4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitogen-activated protein kinase kinase kinase 8</fullName>
            <ecNumber evidence="5">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1W4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004792"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004850.1">
        <property type="protein sequence ID" value="ENSXCOP00000004792.1"/>
        <property type="gene ID" value="ENSXCOG00000003759.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158806"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000327">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF31">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="116"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR038171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="435" mass="48923" checksum="22D3710C5729DBEF" modified="2018-12-05" version="1">MDYKYDTGLELLLAHMNVEDIINGLSQEEMDENEEIGDGVGPGKEQKDYANGLGGVRYGTVQDILSFVNLLSNMQAAALQHLPEEMGVLLNKVRLKGRYQINLDVLLFPWKLTYKNPGCGLVPKGSFGKVHLAQDITTRKRMACKLIPIENFKAADVEFQARFRHENIAELYGAVLWDQTVHLFMEAGEGGSVLEKLDSCGPMREFEIIWVAKQVLRGLEYLHSHKVIHHDIKPSNIVLMSDKAVLVDFGLTVQMTEDMYTPRDLRGTEMYMSPELVLCRGHNTKTDIYSLGTTIIHMQTASPPWVRRYPRTAYPSYLYIIHKQAPPLEDIPEDCSAAMRSFLGRALERNPALRSSASELLKDDALNPPREDQPRCWSLDSALEEATHLLLRQPKSSLYSEDSGHLRRKGSLYIDLGALSGYGKLVTGPPTSEYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1Y4</accession>
    <name>A0A3B5L1Y4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CUL7/CUL9 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1Y4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004350"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004403.1">
        <property type="protein sequence ID" value="ENSXCOP00000004350.1"/>
        <property type="gene ID" value="ENSXCOG00000003433.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153954"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055486">
        <property type="entry name" value="CUL7/CUL9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22771:SF4">
        <property type="entry name" value="CULLIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22771">
        <property type="entry name" value="CULLIN AND GALACTOSE-BINDING DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23168">
        <property type="entry name" value="CUL7_CUL9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUL7/CUL9 N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="220" mass="24459" checksum="DFD2F0FD6B56EF70" modified="2018-12-05" version="1">MVGERRNGNLLVQLGPSLQAYPEELIRQRRTHDGQTEYLIRWCLVTVDDGSTKPENILMWMTMEDVFANCPTLLGKRKAEAQHGAPQGGEEFPADVTFDEVELSDMKQDVKNLVCRARKQMAKKSDFSINIMHTIHVLSAYASIGSLVGVFKETGALNLLMELLCNKETQTRRSAGKMLRALASHDAGQLHSGEPERHGGAHAGGLRGGERRRRGRVPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L333</accession>
    <name>A0A3B5L333_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L333"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004833.1">
        <property type="protein sequence ID" value="ENSXCOP00000004775.1"/>
        <property type="gene ID" value="ENSXCOG00000003757.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000048454"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
    </dbReference>
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        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006574">
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    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="109" mass="12230" checksum="DFAFDE57BACC371B" modified="2018-12-05" version="1">PPPASVVLDPNTAHSNLKLSQELTSVQYSNKLLLPDNPERCTSRMCVLGATGFTSGKHSWTVEVGHDKDWFVGVARESIKRKTTTFLSPEEAYLLLSFWSFWAGVWDFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4H3</accession>
    <name>A0A3B5L4H3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="18">SET domain containing 1B, histone lysine methyltransferase a</fullName>
        </submittedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000005717"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005783.1">
        <property type="protein sequence ID" value="ENSXCOP00000005717.1"/>
        <property type="gene ID" value="ENSXCOG00000004451.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154575"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042800">
        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19169">
        <property type="entry name" value="SET_SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000010">
        <property type="entry name" value="Histone-lysine N-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <begin position="870"/>
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    <sequence length="1810" mass="200046" checksum="067606F0432AC2C3" modified="2018-12-05" version="1">NGESKAISSFCVFKKKHSIGRTMSKPAERNRLNEDHGRKQSSSLANGMDSHPVCGSAEKKSHHWRSYKLIIDPALKKGSHKLYRYDGQTFSMPVSGIPPVDLVRDPRIGRLWTKYKETDLPVPKFKIDEFYVGPVPPKEVTFARLNDNIREGFLTDMCKKFGDIEEVEILYNPKNKKHLGIAKVVFENVKSAKVAVQSLHNTSVMGNIIHVELDPKGENRLRYFQLLMNGSYTPRTLPVGGEENRDVSPRSLAEALLACEPIRRLSESSMSIGGVLPPSSSTTPLSLETGYSSIRQDTPQSQGTPHTPRQPGTPFSQDSSYSSRQSTPAYQSSRPESSGGYKSRRHESKFQDAYNRRPERPQYRSNMYRNTPSDQASFKQHQLTPPEPPPSTPSFNYTAHPPATPNFKSAFSPYQAALPPAFPPSESPYHHPAQRDGEYLRPPQPPPTAPTDVLPVKERPETPPIPEPPPEPVPQPTTPPPQTPERCPSPGSPALDPERNSLDSRIEMLLKEKRTKLLPFLDERDSDNEVRMEGSPISSSSSQLSPIPPFAGGQQNSRPSSTGLEDISPTPLPDSDDEEPISGTASLIKRIGSPINEKMGKSDLKDGAFPGNSPTEKMDTAHQSSGEDMEISDDEMPGATITSGECAKGIVVNSAVSPMHTMSMHPASYHAMQHQAGFAIAPQHLDPHSNMLGHPAHLRGHHGVPPHMISPIGHYHQDVIQMMGLMNCVPWERWNTMHISFQMQQQMLSRMTQTRGPYSYPLFMDGGTSGQFGGLYPPFSLDAAAAAAPACSTGVPGQQWHHPSLPQFNPTVPPPGYETKKEDPHKATVDGVLLVIVKELKAIMKRDLNRKMVEVVAFRAFDEWWDKKEHSAKASLTPVKGAESKEEERPKPKETIGSSLLENWNKGEGLGYEGMGLGMGLRGVKRKDPPDTATGGESKRARPATPADDELEDEAELPSDDSKMAGDGPSAKRRPSRPLELDSEGEEEMDTSEKEEEEESLSDAVDEERDEDKAPDRLLSSADEEDKDKDGDSSSDSGSSDSSDDEADSSASSKASSDSSAESSDSSGESSDSEYEMSSEEEETEEDVKAVAEKVDGKEAETTSSSSSSSTSSSDEEEENKEEDVKPATPPIVQVQEFKEERREEEEQSSKFKLKEEKVCVDVKVEPQDHIAGLRPLTPTGALPDSDQEVKLKGKAKPEEVPCTPTQLPPSHPVAPAPKSISTSSMHLPLPPHPAVESRSLLHLPPGPLPEFPQRPRLPTDEDIPRTPGRDLMERARCLGKSQSTDTIPNTPGSDAPLTGSSLLLSSPHIPGSPFSYPSQSPVLSAGVPRTPGRDLTFTPVFPEPAALTLNRKSFDERPVFKEPPISAPPSLAAGADHSAVAPEDLPTGVSGDIPLMSDATASKKKPGRPKIKKAGDELSSESTDLPQAPVLDSVSLTTVQSPKHTSLDFRERDVDPQTLLPAEDGFPSYDEEPPAVAKPARKTRRCWEELLLDSLSPVTTPQRAYFKPRSDFEEMTILYDIWNEGIDEEDIRLLQITYDKMLQQDNGNDWLNDTLWFWVIFFPLDFCAPTHIPGVKKKRRDDGMRDHATGCARSEGYYKIDKKDKLKYLQSSRVQSEEPPVDTQGMSIPAQVHASTRAGSERRSEQRRLLSSFACDSDLLKFNQLKFRKKKIRFCKSHIHDWGLFAMEPIAADEMVIEYVGQNIRQVIADMREKRYEEEGIGSSYMFRVDHDTIIDATKCGNFARFINHSCNPNCYAKVITVESQKKIVIYSRQPINVNEEITYDYKFPIEDEKIPCLCGAENCRGTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4K1</accession>
    <name>A0A3B5L4K1_9TELE</name>
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        <submittedName>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="2">
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        <property type="gene ID" value="ENSXCOG00000004166.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="89"/>
            <end position="107"/>
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    </feature>
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            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="Ensembl" id="ENSXCOP00000005320.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    <sequence length="138" mass="15915" checksum="BFBCF38E81109884" modified="2018-12-05" version="1">MTVSGREKKGHQQGRLLLQLEVAWAAACYVALLVLCSLCYSNSLYGELVHDDVWAIVNNPDVRPGSSLRNIFRNDFWGKRMADNTSHKSYRPLCILTFNFYLLRILAKVKSYFCYKDFEGIVHSFIALQLNYCNLLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5G7</accession>
    <name>A0A3B5L5G7_9TELE</name>
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        <recommendedName>
            <fullName evidence="3">RCC1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5G7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003004"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003041.1">
        <property type="protein sequence ID" value="ENSXCOP00000003004.1"/>
        <property type="gene ID" value="ENSXCOG00000002383.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166813"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051210">
        <property type="entry name" value="Ub_ligase/GEF_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870:SF155">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HERC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22870">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RCC1-like" evidence="3">
        <location>
            <begin position="4"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="45"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="100"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="155"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="210"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="295" mass="31503" checksum="8C0BF8F6B87E726F" modified="2018-12-05" version="1">MFAGKLGHGDTNRVYRPKVIEALHGFIIRKVCAVSQSSLALTSAGQVFAWGCGSCLGCGSSETTSLRPRFIEDLGITKIIDISCGDSHCLALSHGEKSSPVLYAWGNNTMGQCGQGHTSTPITKPRKVLGLEGISIQQITAGTSHSLAWTEGGKSEVYLWGNGRYGQLAGTGTNLTMPTVAPSLLQTQQVSALVTSCGSDGHSMALTETGEVFTWGDGDFGKLGHGNSERQRRPKQIEALQGEDVVQVNTAGALSCSIWTPRVRYVKSLCCASQAQTCLVCKKRELFFISKIFSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5W6</accession>
    <name>A0A3B5L5W6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ras-related protein Rab</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">The small GTPases Rab are key regulators in vesicle trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endomembrane system</location>
            <topology evidence="6">Lipid-anchor</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027896567.1">
        <property type="nucleotide sequence ID" value="XM_028040766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5W6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005795"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005861.1">
        <property type="protein sequence ID" value="ENSXCOP00000005795.1"/>
        <property type="gene ID" value="ENSXCOG00000004502.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114158869"/>
    <dbReference type="KEGG" id="xco:114158869"/>
    <dbReference type="CTD" id="378969"/>
    <dbReference type="GeneTree" id="ENSGT00940000162477"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090385">
        <property type="term" value="P:phagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04107">
        <property type="entry name" value="Rab32_Rab38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000222">
        <property type="entry name" value="RAB32, member RAS oncogene family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030697">
        <property type="entry name" value="Rab29/Rab38/Rab32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF41">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB-32 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="213" mass="23771" checksum="9D23F06877A33021" modified="2018-12-05" version="1">MAGGSVSECKEYLFKVLVIGELGVGKTSIIKRYVHQLFSQHYRATIGVDFALKVINWDSKTLVRLQLWDIAGQERFGNMTRVYYKEAVGAFVVFDVTRGATFEAVSKWKHDLDSKVKLANGNPIPAVLLANKCDQKKDNVNNSTLMDNFCKETGFLGWFETSAKDNINVDEAARFLVENILANDKGLPYEESNGDRIKLDQETVAAESKSGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L750</accession>
    <name>A0A3B5L750_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA damage-inducible transcript 4-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DDIT4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L750"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006265"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006340.1">
        <property type="protein sequence ID" value="ENSXCOP00000006265.1"/>
        <property type="gene ID" value="ENSXCOG00000004842.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063652"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.40">
        <property type="entry name" value="RTP801-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012918">
        <property type="entry name" value="RTP801-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038281">
        <property type="entry name" value="RTP801-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12478:SF17">
        <property type="entry name" value="DNA DAMAGE-INDUCIBLE TRANSCRIPT 4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12478">
        <property type="entry name" value="DNA-DAMAGE-INDUCIBLE TRANSCRIPT 4 PROTEIN DDIT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07809">
        <property type="entry name" value="RTP801_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="195" mass="22008" checksum="1AA00EAB8730CA80" modified="2018-12-05" version="1">VTVTMVATSKLKIKNTEGVSELVERRYDQVCIEKELDFWGHCMAEPHADVTENRSCHQLAKMFENCLSRAKKTTLHCSSVLVPEKLTRRIAREVLQLASCEPCGLRGCVLYVHLELEKGWKQLERIACDASVVPTFELTLVFKQDGSAWPSLRDFLLMGSCFGPTFRHVIKLSPGFRLVKKKLYSSSAGTVIEKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7E4</accession>
    <name>A0A3B5L7E4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L7E4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005696"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005762.1">
        <property type="protein sequence ID" value="ENSXCOP00000005696.1"/>
        <property type="gene ID" value="ENSXCOG00000002622.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00740000115534"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd23807">
        <property type="entry name" value="UEV_UBE2V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000026">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017175864" description="UBC core domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="UBC core" evidence="3">
        <location>
            <begin position="25"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="165" mass="18668" checksum="630FB1D901BDF221" modified="2018-12-05" version="1">MYLFFNVFLFLSLCVCVRIKGVVVPRSFRLLEELEEGQKGGGDGTVSWGLADDDDMMLTHWNGMIIGPAKVSPLPTVYEGRIYSLKVDCGHRYPEYPPMVRFVNKINLTGVNSSTGAVDLKSVSALSRWKNSYNIKMVLQELRQHMMMKENSRLSQPPEGQVYSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7G5</accession>
    <name>A0A3B5L7G5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nuclear factor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Binds DNA as a homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the CTF/NF-I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L7G5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006380"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006460.1">
        <property type="protein sequence ID" value="ENSXCOP00000006380.1"/>
        <property type="gene ID" value="ENSXCOG00000004936.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182916"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000647">
        <property type="entry name" value="CTF/NFI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020604">
        <property type="entry name" value="CTF/NFI_DNA-bd-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019739">
        <property type="entry name" value="CTF/NFI_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019548">
        <property type="entry name" value="CTF/NFI_DNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003619">
        <property type="entry name" value="MAD_homology1_Dwarfin-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11492:SF4">
        <property type="entry name" value="NUCLEAR FACTOR 1 B-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11492">
        <property type="entry name" value="NUCLEAR FACTOR I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00859">
        <property type="entry name" value="CTF_NFI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03165">
        <property type="entry name" value="MH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10524">
        <property type="entry name" value="NfI_DNAbd_pre-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00523">
        <property type="entry name" value="DWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00349">
        <property type="entry name" value="CTF_NFI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51080">
        <property type="entry name" value="CTF_NFI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0010">Activator</keyword>
    <keyword evidence="3 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CTF/NF-I" evidence="11">
        <location>
            <begin position="2"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="261"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="325"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="353"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="488" mass="54389" checksum="37CF51CC0DE3756B" modified="2018-12-05" version="1">VMYSPICLTQDEFHPFIEALLPHVRAIAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKPEVKQKWASRLLAKLRKDIRQEYREDFVLSVTGKKPPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKSPHCTNPALCVQPHHITVSVKELDLFLAYYVQDQDSQQSGSPSNNEPGKTPLPVYLEDSFIKSGVFSVTELVRVSRMPITHGAAVPFSMADMPSQPYYHDLNSSVALQRSLSSPPGSKRPKTNMEENMDTSPTGPDFYSSPSSPASSRANWHDRDGEMSSPTMKKPEKPLFSPTSPQDSSPRLSTFSQPHHPGLSGVGHSVISSRSPPSHSPLQFSASAILPPAPSSYFSHPTIRYPPHLNPADPLKSYVPSYDPSSPQSSQLRKCDWSVTQNGRHYGPSATDDTLGIAWQSPGTWASLVPFQVSNGTPILPTNVHQNYSINIIGEPLVPAESGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7I8</accession>
    <name>A0A3B5L7I8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L7I8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005736"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005802.1">
        <property type="protein sequence ID" value="ENSXCOP00000005736.1"/>
        <property type="gene ID" value="ENSXCOG00000004472.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154717"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22180">
        <property type="entry name" value="F-box_FBXL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000011">
        <property type="entry name" value="Lysine-specific demethylase 2B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050690">
        <property type="entry name" value="JHDM1_Histone_Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23123">
        <property type="entry name" value="PHD/F-BOX CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="3">
        <location>
            <begin position="119"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="390" mass="44423" checksum="4C5894B9D6852C86" modified="2018-12-05" version="1">LKIRPLKTEDCSSNQNHSPVKPEPGSEADEQKPRWPLSNGSSDLGDWLRHRGREVNGMPHGYSPLSWNRSVPITPICPRPLPRRSPPKCVQMERHVIRPPPISPPPDRLPLNDGEAHVMRREAWMKVFSHLPHRDLCVCMRVCRAWNRWCCDKTLWKQISLNRCKSITPLMLSGIIRRQPVALDLSWTNISKKQLSWLINRLPGLRVLLLSGCSWVAVSALCTSSCPLLRTLDVQWVEGLKDAQMRDLLSPPTDNRPGQLDNRSKLRHVEELRLAGLDITDTTLCLIIRYMPLLSKLDLSYCNHVTDQSVNILTAAGTTTRDSLTDINLSVCNRVTDQSLTYFKRCGSICHIDLRYCKQVTKEGCDQFIAEMSVSVQFELIEDKLLQKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7R0</accession>
    <name>A0A3B5L7R0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">F-box protein 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L7R0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006862"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006950.1">
        <property type="protein sequence ID" value="ENSXCOP00000006862.1"/>
        <property type="gene ID" value="ENSXCOG00000005306.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014416"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22085">
        <property type="entry name" value="F-box_FBXO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039588">
        <property type="entry name" value="FBXO4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16008">
        <property type="entry name" value="F-BOX ONLY PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16008:SF4">
        <property type="entry name" value="F-BOX ONLY PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="45"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="368" mass="41215" checksum="CCE90C474F0C405B" modified="2018-12-05" version="1">MAERNGQLKSVVIRSFRRFSERCFPEKAARSSMLDKEQEEEASHLGYLDSFPVDVQFLIMTFLSPVDICNLGATCRYWRAMVRDPLLWKFFLLRDMPHWSSIDHLTMPDLELLDAPLVSEYLKGCPSCRQQWLPSRPAYELLTSFLQYLVPSSEPRYAMFGPGTEQLDVSLVTRLMHAPDLLPVSGTPHRQINGIGSGISYLFNNQHKFNILTLYSTNKAEREKARLQQQSISNKLFIFEGTDDAGYPVYTPAPQVQKVCQEVDGFIYVANAEPGKGDGASEAAQIRAVLNFVGDSTAKPLLVLSCVSREPEEASEGAKCRTPCVYVAKRLGLPQLSNHWMVQDTVAESLTGVLDGIAWLLKSSGVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L844</accession>
    <name>A0A3B5L844_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine-threonine kinase receptor-associated protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat STRAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L844"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003959"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004003.1">
        <property type="protein sequence ID" value="ENSXCOP00000003959.1"/>
        <property type="gene ID" value="ENSXCOG00000003110.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155197"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032797">
        <property type="term" value="C:SMN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877:SF13">
        <property type="entry name" value="SERINE-THREONINE KINASE RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="8"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="131"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="198" mass="21957" checksum="AD3C7E53A843DCF5" modified="2018-12-05" version="1">ARLTIDLIAGHSSAIKKSLWCNDDSQILSAAEDKTVRLWDSRSMEEVKTLTFDTSVSSMEYLADGETLVITYGKTIAFYNALSLDLIKTVEAPAPINSASLHPEKDFFVAGGDDFKLYKFDYSTKEELESYKGHFGPVHCVRFSPDGELYASGSEDGTLRLWQTAVGKTYGLWKCVLPEDLGTENSEQLYSSTPEIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8T1</accession>
    <name>A0A3B5L8T1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ornithine aminotransferase</fullName>
            <ecNumber evidence="10">2.6.1.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reversible interconversion of L-ornithine and 2-oxoglutarate to L-glutamate semialdehyde and L-glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-ornithine + 2-oxoglutarate = L-glutamate 5-semialdehyde + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:25160"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="EC" id="2.6.1.13"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:25161"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="7">
            <dbReference type="Rhea" id="RHEA:25162"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 2-oxocarboxylate + L-ornithine = L-glutamate 5-semialdehyde + an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:13877"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="EC" id="2.6.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.13" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8T1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007227"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007319.1">
        <property type="protein sequence ID" value="ENSXCOP00000007227.1"/>
        <property type="gene ID" value="ENSXCOG00000005587.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00630000089895"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00358"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004587">
        <property type="term" value="F:ornithine aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019544">
        <property type="term" value="P:L-arginine catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010121">
        <property type="term" value="P:L-arginine catabolic process to proline via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000011">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000152">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010164">
        <property type="entry name" value="Orn_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01885">
        <property type="entry name" value="Orn_aminotrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF18">
        <property type="entry name" value="ORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="437" mass="48339" checksum="636B0E9258E568F7" modified="2018-12-05" version="1">LIGLYSMSRGISFMRVSFSHFLRSSINTHPRPATVTKLTSEEIYAREEKHGAHNYHPLPVALERGIYVWDVEGNRYYDFLSAYSAVNQGHCHPKIVSALTKQASTLALTSRAFYNDVLGAYGEYITNMFGYDKVLPMNTGVEGGETACKLARKWAYNIKGVPNNKAKIIFAEGNFWGRTLAAISSSTDPSSYEGFGPYMPGFELVPFNDIPALEKAFQDQNVAAFMVEPIQGEAGVVVPDQGYLTKVRELCTKYNVLWIADEVQTGLARTGRRLAVDHEGVRPDLVILGKALSGGVYPVSAVLCDNDIMLTIKPGEHGSTFGGNPVACRVAIAALEVMEEEKLAENAQRMGELLRSELRKLPKAIVTTVRGKGLLNAVVIKETKDYDAWKVCLRLRDNGLLAKPTHGDIIRLAPPLVIREHELQECIDIIQRTIMSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LA45</accession>
    <name>A0A3B5LA45_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Procollagen C-endopeptidase enhancer a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027886960.1">
        <property type="nucleotide sequence ID" value="XM_028031159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LA45"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007717"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007814.1">
        <property type="protein sequence ID" value="ENSXCOP00000007717.1"/>
        <property type="gene ID" value="ENSXCOG00000005930.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114152970"/>
    <dbReference type="KEGG" id="xco:114152970"/>
    <dbReference type="CTD" id="563867"/>
    <dbReference type="GeneTree" id="ENSGT00940000159264"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016504">
        <property type="term" value="F:peptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd03576">
        <property type="entry name" value="NTR_PCOLCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000013">
        <property type="entry name" value="Membrane frizzled-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018933">
        <property type="entry name" value="Netrin_module_non-TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035814">
        <property type="entry name" value="NTR_PCOLCE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF48">
        <property type="entry name" value="PROCOLLAGEN C-ENDOPEPTIDASE ENHANCER A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01759">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00643">
        <property type="entry name" value="C345C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017274119" evidence="9">
        <location>
            <begin position="24"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="10">
        <location>
            <begin position="35"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="10">
        <location>
            <begin position="157"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="11">
        <location>
            <begin position="345"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="276"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="328"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="464" mass="51036" checksum="1DDF886E93CD406B" modified="2018-12-05" version="1">MMRVDCIWGLLLLLSLSLGWTKAQETNYTRPVFYCGGDLVADSGFVGSEGFPSFYKPNSKCTWRITVPEGNVVALSFRIFDLEADSQCRYDYLDIYNGHSNLVQKLGRFCGTFRPGTQVSTTNTMMLEMVTDEGNQARGFLAHFSGTKPYVNGEQSCGGKMTKSQGEIKTPNWPDGKYPPGTTCSWLITVEPDRVIQVKFDKFVLEADTYCRFDYVAFFNGGERDDSRLIGKYCGDQTPQPIITNGNELLVQFVSDLSVTSDGFFAYYTSIPRGSEPPIDKSRPRVGSAPSRRPVKPARPEGPAITARVPIPTTTAAPKYVPPPPVSPTRRVPNRNRPGPQKPLCPRVCKRAGTIKNSFCSSEFVITGTITSLGPGPRGSVSMNISLIKAYKSGRLTITQAGETMSVKLVSQCKRCPLVRRGANYILMGQVDEEGRGTLAPGAFTAPYKVAHHKLLMNINNQPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAF9</accession>
    <name>A0A3B5LAF9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Uracil nucleotide/cysteinyl leukotriene receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">G-protein coupled receptor 17</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Dual specificity receptor for uracil nucleotides and cysteinyl leukotrienes (CysLTs). Signals through G(i) and inhibition of adenylyl cyclase. May mediate brain damage by nucleotides and CysLTs following ischemia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAF9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007822"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007920.1">
        <property type="protein sequence ID" value="ENSXCOP00000007822.1"/>
        <property type="gene ID" value="ENSXCOG00000006011.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286998"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15161">
        <property type="entry name" value="7tmA_GPR17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000152">
        <property type="entry name" value="uracil nucleotide/cysteinyl leukotriene receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047160">
        <property type="entry name" value="GP183-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24237:SF42">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24237">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="196"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="239"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="51"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="344" mass="38249" checksum="204EF5E3DCB54719" modified="2018-12-05" version="1">MSIMTKWSATTAETSLLSNQSSESCVPAETAIQNQLFGWFYVVVFVLALGGNSLALWIFSRQRGVFSPANVFLVHLAVADLSYVVILPLRATYHFTGGHWPLGEVPCRLVGFLFYVNMYASLYFLACVAGDRYLAVVHAVRSMKLRQGRYAHIISLCLWVLVTLSMAPLLVNHQTAQVDNTTVCLQLYREKVSRRALVSLAVAFTPPFLATLSCYLLIIHSLHRGCRLDPALKLKALRTIGAVILIYMVCFLPYHLSRVTFILGYNNPDVSCQTQRALSMANRFTSSLTCMNGAMDPLVYLFGAEKFRSTLKRLFCKDKAGMIGATSGDLKETHESSLSAKSEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LB80</accession>
    <name>A0A3B5LB80_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">E2f-associated phosphoprotein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LB80"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006971"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007059.1">
        <property type="protein sequence ID" value="ENSXCOP00000006971.1"/>
        <property type="gene ID" value="ENSXCOG00000005388.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001332"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019370">
        <property type="entry name" value="E2F-assoc_phosphoprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15967">
        <property type="entry name" value="E2F-ASSOCIATED PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15967:SF0">
        <property type="entry name" value="E2F-ASSOCIATED PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10238">
        <property type="entry name" value="Eapp_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="265" mass="30341" checksum="EEE34C03BA7A8E9E" modified="2018-12-05" version="1">MNKLSKPQEFDSYEIEEPSDEERAESSSEDELDVLLNGTPEQKKKLIREYLTGESESSSEDEFEKEMEAELSSTIRTMEGTWGPAAGSAGASCVSKPQMYDKVYFDSDSEDDDTPGKRMVLSNDELLYDPDEDDRDQAWVDARRYQHVLFSAMKVSDAVLNCPACMTTLCLDCQRHEKYRTQYRAMFVMNCTVKTDEVLRYKTQQEKKPRKRRRGQTAEGASPRPAGMDSDEIYHPVKCSECSTEVAVFDKDEVYHFFNILASHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCP3</accession>
    <name>A0A3B5LCP3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="RefSeq" id="XP_027877859.1">
        <property type="nucleotide sequence ID" value="XM_028022058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCP3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008310"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008410.1">
        <property type="protein sequence ID" value="ENSXCOP00000008310.1"/>
        <property type="gene ID" value="ENSXCOG00000006365.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114147575"/>
    <dbReference type="KEGG" id="xco:114147575"/>
    <dbReference type="CTD" id="559882"/>
    <dbReference type="GeneTree" id="ENSGT00950000183077"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000117">
        <property type="entry name" value="Probable E3 ubiquitin-protein ligase makorin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045072">
        <property type="entry name" value="MKRN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224">
        <property type="entry name" value="MAKORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224:SF39">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="5"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="192"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="274"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="5"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="274"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="352" mass="40166" checksum="D386946F4780F668" modified="2018-12-05" version="1">MDSVRSGNICRRFINGSCRFGRRCKYRHEFTPIPASQICRYFQKGGCWYGERCRFFHVLLPQVGPVFPGRRGSVPVASPSGMPHYSERRGSEPSLWRFNVPSRHRCSGSQSAIYPFNLQQETGNLPEYVLEELSQENYLESAPSSGIAEASAWQGRYERASKSETCEDGAAAACNEKSEETLAFLQSKNVVCGICMDKVYEKENQRDRVFGILPNCNHPFCLDCIATWRKTKDIGLDVVRACPQCRVRSAFYVPNKYWVEGQEKETVIGAFKKKFGKKRCCFYDRYGCCPFKKECLYRHVGDSTNSPVFSYLSEEEDDNFTDLLGFFISMSLLGGEEADEELLFCLTEDYGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LD89</accession>
    <name>A0A3B5LD89_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Fb</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LD89"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005834"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005900.1">
        <property type="protein sequence ID" value="ENSXCOP00000005834.1"/>
        <property type="gene ID" value="ENSXCOG00000004484.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156703"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038191">
        <property type="term" value="F:neuropilin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003205">
        <property type="term" value="P:cardiac chamber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003170">
        <property type="term" value="P:heart valve development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097350">
        <property type="term" value="P:neutrophil clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05871">
        <property type="entry name" value="Ig_Sema3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11254">
        <property type="entry name" value="Sema_3F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000030">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000001">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF27">
        <property type="entry name" value="SEMAPHORIN-3F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Sema" evidence="10">
        <location>
            <begin position="31"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="617"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="801" mass="90547" checksum="3290BEF46D5C4BF7" modified="2018-12-05" version="1">MLLCRSGMRSMSVVCMRKCAYINVFGCHKRNRFCLHTELRSTGTAHHFAYLLNASDYRILRMDEDHDRMYVGSKDHILSLDLHDINKSPHIIHWPVSERRRTECLVSGKDASEECANFIRLIEPWNRTHLYICGTGAYNPVCTFVNRGRKPQTSMYLQMALAGGRASRAADPSAVHDTLGLKEEIFHLEPGKVESGKGKCSYDPKLNSVSALINGELYAGVYVDFMGTDSAIFRTLGTKTAMRTDQYNSRWLNDPTFVQVHLIPDSAERNDDKLYFFFREKSSDMGQSPVTQSRIGRICLNDDGGHCCLVNKWSTFLKARLICSVPGTDGMETHFDELRDVYIQPTQDTKNPVIYGVFTVSGSVFKGSAVCVYSMADIRMVFNGPFAHKEGPNYQWVAYTGKIPYPRPGTCPGGTFTPSMKSTKDYPDEVINFMRNHPAMHNAVYPVHKRPLVVRTNVDYEFTTISVDQVTAADGNYEVLFLGTDKGTVQKVIVLPRDDLQTEELVLEEVEVFKVPTPITTMKISSKRQQLYVSSAVGLTQLALHRCDVYGEACADCCLARDPYCAWDGKSCSRYSASQKRRSRRQDVRYGNPIRQCRGFNSNANKNTLEMVQYGVEGSSTFLECQARSPHAVIKWHLQRDNSDRRKEMRSDGRILKTEQGLLLRSLQPSDSGMYQCTATEKNFKHTLVKLQLVVLSGRAVNNALVDGGGLVPSSLHSAGAQWTPSAGQYKDLLTILSQPEMSLINQYCQDYWQFGDPLLGALKAKDLKELKEQKKPRNRRHNYEQPHPSCFFFFFPSEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDE1</accession>
    <name>A0A3B5LDE1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Vesicle-associated membrane protein 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDE1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007711"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007808.1">
        <property type="protein sequence ID" value="ENSXCOP00000007711.1"/>
        <property type="gene ID" value="ENSXCOG00000005898.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164382"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15870">
        <property type="entry name" value="R-SNARE_VAMP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000013">
        <property type="entry name" value="Vesicle-associated membrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016444">
        <property type="entry name" value="Synaptobrevin/VAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45701">
        <property type="entry name" value="SYNAPTOBREVIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005409">
        <property type="entry name" value="Synaptobrevin_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="26"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="111" mass="12240" checksum="B61ACF5C2B96079B" modified="2018-12-05" version="1">RSAPAAGAPAPEGGSQQPPNLTSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKNKYWWKNAKMMIILGVICVIVLIVIIVYFST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LF59</accession>
    <name>A0A3B5LF59_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dynein heavy chain hydrolytic ATP-binding dynein motor region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LF59"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008326"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008431.1">
        <property type="protein sequence ID" value="ENSXCOP00000008326.1"/>
        <property type="gene ID" value="ENSXCOG00000006386.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157623"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000044">
        <property type="entry name" value="Dynein heavy chain 5, axonemal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF32">
        <property type="entry name" value="DYNEINS HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region" evidence="13">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF12774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="210" mass="23010" checksum="A447AC9643CA3212" modified="2018-12-05" version="1">VITPLTDRCYMTLTTALHLHRGGSPKGPAGTGKTETVKDLGKSLGMYVIVVNCSEGLDYKSMGRMFSGLAQTGAWGCFDEFNRINIEVLSVVAQQILSILSALTAEKSKFLFEGHTIRLVPTCGIFITMNPGYAGRTELPDNLKSMFRPISMVVPDSTLIAEILLFGEGFDNCKVLYLCDSKNYREIVLNTISATFLPVFCTIFSISLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFH6</accession>
    <name>A0A3B5LFH6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFH6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009330"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009441.1">
        <property type="protein sequence ID" value="ENSXCOP00000009330.1"/>
        <property type="gene ID" value="ENSXCOG00000007105.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155053"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990716">
        <property type="term" value="C:axonemal central apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035082">
        <property type="term" value="P:axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050995">
        <property type="entry name" value="WD-F-box_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14604:SF3">
        <property type="entry name" value="SPERM-ASSOCIATED ANTIGEN 16 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14604">
        <property type="entry name" value="WD40 REPEAT PF20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="60"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="102"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="144"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="228"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="299" mass="31501" checksum="670DBC5C5D8C29DD" modified="2018-12-05" version="1">MEPCGLPAGPEAPEKSSFQLFCSFRAHQLPISGVAPHPGRPLLASVRLWDFSACRCVLLLAGHSQPTWGSSFHSSGQILASCSADGTVRMWDPSRGCCRMILRRHGASVNSVAFLPCSDLLLTSSADKTVVVWDGRLGVSTATFRGHRHPCNHAAFSPTTNAVASCDSRGTVHLWDTRKPAAPTGIVDAGPHAANQVAFNQSGKLLAVASGDCLVRLVEAESCGVSTLAGHRKSVQSVTFDLQGGTLMSAGSDGLINVLCFGFKIPQHDAATTVPHLSCGSLGPNLTKNVKKQQILLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFV9</accession>
    <name>A0A3B5LFV9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pleckstrin homology-like domain, family A, member 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFV9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009742"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009855.1">
        <property type="protein sequence ID" value="ENSXCOP00000009742.1"/>
        <property type="gene ID" value="ENSXCOG00000007409.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000039564"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901981">
        <property type="term" value="F:phosphatidylinositol phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042832">
        <property type="entry name" value="PHLA1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15478:SF12">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY-LIKE DOMAIN FAMILY A MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15478">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY-LIKE DOMAIN, PQ-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="5"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="140" mass="16081" checksum="78D8843DEFE11EEF" modified="2018-12-05" version="1">TVESVIRDGLLEKRSSGLLQLWKKKRCVLTEEGLRLLSCKGRGSDAPGSAWSSRAKELRFERMETVECVEYKREMVYFTVVMAKGKEIDFRCAQEGTLWNAEIALALVRYKNQQKSLRAKQKLQLTLRSDHSFQSAFVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHC8</accession>
    <name>A0A3B5LHC8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Mgat4 family member C</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHC8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007269"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007362.1">
        <property type="protein sequence ID" value="ENSXCOP00000007269.1"/>
        <property type="gene ID" value="ENSXCOG00000005622.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155352"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008375">
        <property type="term" value="F:acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006759">
        <property type="entry name" value="Glyco_transf_54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057279">
        <property type="entry name" value="MGAT4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056576">
        <property type="entry name" value="MGAT4_A/B/C_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062:SF14">
        <property type="entry name" value="ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 4-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYLTRANSFERASE VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04666">
        <property type="entry name" value="MGAT4_cons"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23524">
        <property type="entry name" value="MGAT4A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="MGAT4 conserved region" evidence="5">
        <location>
            <begin position="67"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="MGAT4 A/B/C C-terminal" evidence="6">
        <location>
            <begin position="344"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="483" mass="56255" checksum="43494AB72D1B7DDE" modified="2018-12-05" version="1">STKFHLMRLVWKSLDKMRCLRKRSTIPFLGFLITFLLFLNLYIEDGYVLEGDKRQLREASVHPPSSERYAHTFRDLSNFSGTINVTYRYLAGTPLNRKKFLTIGLSSVKRKRGNYLLETIKSIFDQSSYEELKEIVVVVHLADFDLVWCENVVQEITRKFAHHIIAGRLLVIQAPEEFYPSLDGLKRNYNDPEDRVRFRSKQNVDYAFLLNFCTNLSHFYMMLEDDVRCSRNFLTALKKVITSREGSYWVMMEFSKLGYIGKLYHSRDLPRLAHFLLMFYQEMPCDWLLIHFRGLLAQKDVIRFKPSLFQHMGYYSSYKGVENKLKDDDFEEDSIDIPDNPPAGIYTNINVFENYDATKAYSTVDEYFWGKPPSTGDFFVIVFNKSTKISKIRIATGSDERQSDFLHHGALEVGEKLVGTKKGKQCSSYITLGEFKNGNIEVQDVDHKISFDVECVRIVVTASQKEWLIIRSISLWTTQPPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHD3</accession>
    <name>A0A3B5LHD3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dedicator of cytokinesis 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHD3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007274"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007368.1">
        <property type="protein sequence ID" value="ENSXCOP00000007274.1"/>
        <property type="gene ID" value="ENSXCOG00000005628.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154974"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007520">
        <property type="term" value="P:myoblast fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.740:FF:000004">
        <property type="entry name" value="Dedicator of cytokinesis protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.410:FF:000004">
        <property type="entry name" value="Dedicator of cytokinesis protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046769">
        <property type="entry name" value="DOCKER_Lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056372">
        <property type="entry name" value="TPR_DOCK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653:SF1">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06920">
        <property type="entry name" value="DHR-2_Lobe_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23554">
        <property type="entry name" value="TPR_DOCK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DOCKER" evidence="4">
        <location>
            <begin position="566"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1047" mass="122591" checksum="5A1288C7312AEDB9" modified="2018-12-05" version="1">LQLSVLCERIALFKENQKQSSFLVCLKTFMWKKVLRSNQTKKLEDSSVYLSLPSTKLELEEKGYANTGKNSQNLGTCTISKDSFQIATLVCSTKLTQNVDLLGLLKWRSNTSLLQQNLCQLMKVDGGEVVKFLQDTLDALFNIMMENSDSDTFDTLVFDALVFIIGLIADRKFQHFNPVLETYIRKHFSATLAYTKLTKVLKNYVENAEKLTEQLLMAMKALEYIFKFIVRSRVLFNQLYENKDEAEFMNSLRSLFTSFNDMMNSNSENTGMVKVGLLSHESNCVLSLKFPARKYCREILLPLMTEQLKSHLEKQEEPKACCQLLSDIMEVLYRKDVVRNTTHMHDMLYSHQRVFLRAISQYADVLSKKFLDQANFELQLWNNYFHLAVAFLTQESLQLENFSSDKRTKIFQKYQDMRRQIGFEIRDMWYNLGPHKIKFIPEMVGPVLEMTLVPEIELRKATIPIFFDMMQCEFHFTCGFQRFENEIITKLDHEVEGGRGDEQYKVLFQKILLEHCRKHKYLARTGENFVTLVVRLLERLLDYRTIMHDENKENRMSCTVNVLNFYKEIDREEMYIRYLYKLCDLHRECDNYTEAAYTLLLHAKLLKWSDEQCAAHLTQREGYQATTQGQLKDQLYQEIINYFDKGKMWEEAIVLSKELAEQYENEMFDFEQLSASLRKQAQFYENIVKVIRPKPDYFAVGYYGMGFPSFLRNKMFMYRGKEYERREDFEARLLTQFPNAEQMKTTTPPSEDTKSSPGQYIQCFKVKPILNLPAKFQNKPVSEQIVSFYTVNEVHKFQYSRPVRKGEKDPDNEFANMWLERTTYTTAYKLPGILRWFEVKSVSTEISPLENALETMQLANEKISSMVQRHLSDPNLPINPLSMLLNGIVDPAVMGGFANYEKAFFNDKYMQEHPEDHEKIEKLKDLIAWQVRLKLSETLSVVFFILFASVFALGCCSSRKTAVIALSQVVYFEKMIHSVFIITVSPHFEKYMSLVCRDTQPRALSSYEFSSELIYIFPSTALSSFSVNCHNTQLYYATIKETLYANF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LI60</accession>
    <name>A0A3B5LI60_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Stomatin (EPB72)-like 3a</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LI60"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007544"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007640.1">
        <property type="protein sequence ID" value="ENSXCOP00000007544.1"/>
        <property type="gene ID" value="ENSXCOG00000005810.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234614"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000002">
        <property type="entry name" value="band 7 protein AGAP004871"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018080">
        <property type="entry name" value="Band_7/stomatin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="BAND 7 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264:SF87">
        <property type="entry name" value="STOMATIN (EPB72)-LIKE 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01270">
        <property type="entry name" value="BAND_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="5">
        <location>
            <begin position="40"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="271" mass="30216" checksum="7FF3DD7821AD0261" modified="2018-12-05" version="1">TLNFSLSTDKNARRLECFGWLLVIISLLFITLTFPVTLFMCVKIVKEYERAVIFRLGRIVDRKPKGPGLFFVLPCTDNFVKVDLRTVSFDIPPQEILTKDSVTVSVDGVVYFRIHCPISAVANVSNAHTSTRLLAQTTLRNVLGTKNLSELLSDREGISVSMQEALDEATDSWGIKVERVEIKDVKLPQQLQRAMAAEAEASREARAKIIAAEGEMNASRALKEAALVINEAPSALQLRYLQTLNSIAAEKNSTIIFPLPIDMMQSFMQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJ87</accession>
    <name>A0A3B5LJ87_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJ87"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012148"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012287.1">
        <property type="protein sequence ID" value="ENSXCOP00000012148.1"/>
        <property type="gene ID" value="ENSXCOG00000009189.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156339"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03342">
        <property type="entry name" value="TCP1_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000038">
        <property type="entry name" value="Chaperonin containing TCP1 subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000029">
        <property type="entry name" value="Chaperonin containing TCP1 subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000004">
        <property type="entry name" value="T-complex protein 1 subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012722">
        <property type="entry name" value="Chap_CCT_zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02347">
        <property type="entry name" value="chap_CCT_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="389" mass="42642" checksum="005BBF835E922433" modified="2018-12-05" version="1">MDRETLVNVARTSLRTKVHTELADLLTEAVVDAVLAISKPNEPIDLYMVEIMEMKHKTESDTQLIKGLVLDHGARHPDMKKRVEDAYVLTCNVSLEYEKTEVNSGFFYKSAGEREKLVAAERKFIEERVQKIIALKKKVCTSDDKGFIVINQKGIDPFSLDALAKEGIVALRRAKRRNMERLSLACGGIAMNSLDDLTPECLGHAGLVYEHTLGEEKYTFIEKCGNPRSVTLLVKGPNKHTLTQIKDAVRDGLRAVKNAIEDGCVVSGAGAFEVAVADALVKHKPNVKGRAQLGVQAFADALLVIPKVLAQNSGYDPQETLLKLQTEYKESGQLVGVDLCTGEPMVAGEAGVWDNYSVKKQLLHSCTVIASNILLVDEIMRAGLSSLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJF5</accession>
    <name>A0A3B5LJF5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJF5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011022"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011150.1">
        <property type="protein sequence ID" value="ENSXCOP00000011022.1"/>
        <property type="gene ID" value="ENSXCOG00000008327.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000174883"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050413">
        <property type="entry name" value="TCR_beta_variable"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268:SF28">
        <property type="entry name" value="T CELL RECEPTOR BETA VARIABLE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268">
        <property type="entry name" value="T-CELL RECEPTOR BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017350044" description="Ig-like domain-containing protein" evidence="3">
        <location>
            <begin position="22"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="20"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="138" mass="15988" checksum="2DDF702626E05ED7" modified="2018-12-05" version="1">METENPFCFWLFCLFYFSISAVNFQQLPSVFVRDGDQSVTLRCDQDNNQHYYMFWYRHPAGSGKMELVAYSAGQDTTSVEAPFSKTKYTMTRPEVLRTSLQIHSPEAADSAVYYCASSLAQCFRLPQPPNNNLRRRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LK88</accession>
    <name>A0A3B5LK88_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IL-17 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LK88"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012488"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012639.1">
        <property type="protein sequence ID" value="ENSXCOP00000012488.1"/>
        <property type="gene ID" value="ENSXCOG00000009438.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175981"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010345">
        <property type="entry name" value="IL-17_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06083">
        <property type="entry name" value="IL17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017179616" evidence="5">
        <location>
            <begin position="22"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="183" mass="21020" checksum="2CB9621E73768376" modified="2018-12-05" version="1">LQLTRAFLLLGFVSVIPVASKKRIISVHQIRPRSKTMQLALDPSVKSYLSHTSASNIANLSLSPWTYIDSYNSSRLPQKILQANCSTFGCLNLQGRGEDATLQVKPIKYQVLVLTLKKRNNNKKGKKQNKYIFMLGTEEITVGCTYIQQKFWLHISFMSMSMHFKSCTVDNRYFYMLQEKKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LL49</accession>
    <name>A0A3B5LL49_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Synembryn</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Protein Ric-8</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Chaperone that specifically binds and folds nascent G alpha proteins prior to G protein heterotrimer formation. Also acts as a guanine nucleotide exchange factor (GEF) for G alpha proteins by stimulating exchange of bound GDP for free GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with some GDP-bound G alpha proteins. Does not interact with G-alpha proteins when they are in complex with subunits beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cell cortex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the synembryn family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LL49"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010286"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010409.1">
        <property type="protein sequence ID" value="ENSXCOP00000010286.1"/>
        <property type="gene ID" value="ENSXCOG00000007688.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014700"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001965">
        <property type="term" value="F:G-protein alpha-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008376">
        <property type="entry name" value="Chaperone_Ric-8_A/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019318">
        <property type="entry name" value="Gua_nucleotide_exch_fac_Ric8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12425">
        <property type="entry name" value="SYNEMBRYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12425:SF3">
        <property type="entry name" value="SYNEMBRYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10165">
        <property type="entry name" value="Ric8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01802">
        <property type="entry name" value="SYNEMBRYN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="515"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="520"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="548" mass="62012" checksum="1A4C7ADEAB4E097D" modified="2018-12-05" version="1">MAPDVEKIIQSISLGDQDAVQLLLDSYNTQYAECFFFNTEAQERKKFRKNKIRDCASNSDSDIDSDDKRPNLLLRELQDLAAALLCFVGGQLQPAVLRACLHTLRILSRDRRALGPMVTDSSLLTLTHLGGINLLLQGLWESLKQGIWSGAPPTGQFYKLRLLFLLTALRPELRLQLQQERGVFVLTSALEQCLVLRWADGNQLITDSTSVSKEVSQDVIEILKTIFNVAHRFYRQQEEATLYRRLAAVLRHCLLLSCDGADTMEELQHTVNILSALPLTCLDVMLAVPVGQASHQYEGVNMDCVHALLLFMDRRLNRQGQKLKEKLTPVLSLLTESSRVHRETRHYLRQKILPPLKDVAVRPEQDSTLRGQLVRLMTHVDTDVKDCAAELLFVLCKENSRFVKYTGYGNAAGLLAARGLLNGRRNSGDGQFASHYSSDSDSDTEEYRQVRSKINLVTGRVEAEQPNPMEGMTEEEKEEEACRLICMINRLSDQIIQPMGVTRDGRLAPLWGQMRSCTEEKEEEEDEYEEEEQGLSSMPQGLKDKTMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNB1</accession>
    <name>A0A3B5LNB1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNB1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012210"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012356.1">
        <property type="protein sequence ID" value="ENSXCOP00000012210.1"/>
        <property type="gene ID" value="ENSXCOG00000009249.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168520"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042130">
        <property type="term" value="P:negative regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042102">
        <property type="term" value="P:positive regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031295">
        <property type="term" value="P:T cell costimulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051713">
        <property type="entry name" value="T-cell_Activation_Regulation"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466:SF9">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25466">
        <property type="entry name" value="T-LYMPHOCYTE ACTIVATION ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="32"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="143" mass="16353" checksum="6A2D659E377AB5A6" modified="2018-12-05" version="1">LLFCSKAETDDRRAERETTTLVATSSVCLPLPLLSLYCVISSSTDQISTELQFEPGQNINLKCRTPDNKPAAVVEWNRTDLEDEFVLLFRDGKLDPEHQHQMYENRVDLRDQQMKDGDVSLVLNNMVPDDRGTYECRVAQCRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQ46</accession>
    <name>A0A3B5LQ46_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tetratricopeptide repeat protein 38</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TTC38 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQ46"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009904"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010022.1">
        <property type="protein sequence ID" value="ENSXCOP00000009904.1"/>
        <property type="gene ID" value="ENSXCOG00000007512.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002669"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd05804">
        <property type="entry name" value="StaR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033891">
        <property type="entry name" value="TTC38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16263">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16263:SF4">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="322" mass="36594" checksum="1BCD3192229E2455" modified="2018-12-05" version="1">MQCVIIRSLFALNQIVFYVCFSVKSTLFPVFSKSLFAFSYLKGQYSFGLLETRFYDQALKVAMEGLALTPDDAWAVHSVAHVYEMKAEVDKVLNFMDSREKDWQGSDMLASHNYWHWALCFLEGGEYEAALQIYDSQIFKRCKASGAMLDVVDACSMLYRLEMEGVCVKERWRELLQVTQPHTDDHVTLFNDLHFLMVALGADEDGAAQRLLEGLQELAREPGDNQQHQLAKSLGLPMCEAMMEYKQGNFSQAVALLHPLRYRMVDIGGSDAQLFSQLEVWLNLLVSALLCFPRCLLVERDAVRPNSPLTQRLTQKALALHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQR3</accession>
    <name>A0A3B5LQR3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dachshund d</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQR3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013130"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013291.1">
        <property type="protein sequence ID" value="ENSXCOP00000013130.1"/>
        <property type="gene ID" value="ENSXCOG00000009952.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001134"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052417">
        <property type="entry name" value="Dachshund_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12577">
        <property type="entry name" value="DACHSHUND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12577:SF14">
        <property type="entry name" value="DACHSHUND HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="80"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="178" mass="20581" checksum="1D28E85CF21F8A19" modified="2018-12-05" version="1">YQERVQDTPSPSPSLEEAQMSSNHSSSVSSSPLHNERTAETTRTYSPVLPALPAGLHHTPFIFPEGLSSIETLLTNIQGLLRVAIENARAQEKQDQLERTELKMELVRERELRETLERQLSMEQKNRVLIQKRLKKEKRSKRKLQEALEEEVKLRDQAEHTLLHTVLKDFTARPLLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSQ7</accession>
    <name>A0A3B5LSQ7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibroblast growth factor</fullName>
            <shortName evidence="2">FGF</shortName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the heparin-binding growth factors family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027888609.1">
        <property type="nucleotide sequence ID" value="XM_028032808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSQ7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010839"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010964.1">
        <property type="protein sequence ID" value="ENSXCOP00000010839.1"/>
        <property type="gene ID" value="ENSXCOG00000008188.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114154047"/>
    <dbReference type="KEGG" id="xco:114154047"/>
    <dbReference type="CTD" id="2250"/>
    <dbReference type="GeneTree" id="ENSGT00940000158449"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002209">
        <property type="entry name" value="Fibroblast_GF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11486">
        <property type="entry name" value="FIBROBLAST GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00167">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00263">
        <property type="entry name" value="HBGFFGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00442">
        <property type="entry name" value="FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00247">
        <property type="entry name" value="HBGF_FGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017100047" description="Fibroblast growth factor" evidence="2">
        <location>
            <begin position="20"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="180"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU049442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="225" mass="25602" checksum="F646273B2E687D12" modified="2018-12-05" version="1">MNVPLCLLTFAHWICAAAAAQRASLLLQEGISSGRRTCRLYCRVGIGFHLQIHPDGRVNGSHEPNQLSVLELIAISQGVIGIKGVYSNRFLAMNKRGRLHATEWFSDDCKFRERFQENSYNTYASVIHRNQRTGREWYVALNKRGKAKMGSSPRVKSQHVSTHFLPRASLHDFTQKGFTITERSKERRKAPPPSPPRPPAVIPKAPTGPVQRRIQVKYWPKFRFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LT47</accession>
    <name>A0A3B5LT47_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LT47"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014168.1">
        <property type="protein sequence ID" value="ENSXCOP00000013995.1"/>
        <property type="gene ID" value="ENSXCOG00000010590.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286977"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000759">
        <property type="entry name" value="zinc finger protein 16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001325">
        <property type="entry name" value="zinc finger protein 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041697">
        <property type="entry name" value="Znf-C2H2_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818:SF163">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
        <property type="entry name" value="ZINC FINGER PROTEIN ZIC AND GLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16622">
        <property type="entry name" value="zf-C2H2_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="117"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="344"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="85"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="369" mass="42424" checksum="2EB1E1BF309764C6" modified="2018-12-05" version="1">MLISVLNDFFDKVNNSYFYLFYCPDLLEHNIIKEEPCKQERSFTPDQEELEPLQVKQEDEKTECQQIKEEQEELDYLQIKEAAEAENQNQERTRQHGDSSKQKGHENLPQSSDLTKKTCERPVSSRKRFSRKDKLNRHMSDHTGEKPLLCVNCGKGFSCNSDLTVHMRVHTGEKPFLCVKCGRSFSRRSNLTVHMKAHTGEKQFQCITCGKHFISKGSLIIHVRRHTGEKPFQCKTCGKRFVDQYSVNLHMRSHSVLKPYICVNCGKSFAQKQNLTSHMMIHTGEKPFSCVTCGKCFSQKGILTRHMLIHTGERPFQCLTCGKGFNQKCSLTDHMTIHTGEKPFSCSACGKGFSRRHALTKHMKSHVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LT99</accession>
    <name>A0A3B5LT99_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LT99"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015233"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015424.1">
        <property type="protein sequence ID" value="ENSXCOP00000015233.1"/>
        <property type="gene ID" value="ENSXCOG00000011537.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159122"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002080">
        <property type="term" value="C:acrosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001675">
        <property type="term" value="P:acrosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007338">
        <property type="term" value="P:single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051176">
        <property type="entry name" value="Cent_Immune-Sig_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 170 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715:SF26">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="361"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="97"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="258"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="385" mass="43750" checksum="A134C6895CDB290C" modified="2018-12-05" version="1">MLADTLTAKERESMEEKEEPEGDGEKGQQEEEMEVKERESEEDDQGEEEDLEELRAQVLQLLLELDETREVSQRHEESFMELQGLLDEERLASAHQAESFTRQIQRLQAQLRSAQEEMSSLEEEKESELVEVQQELRSAQEEVLVLQQAAEEAAAERENDIASLQEELCRLRVELQRLHATAAEYELEVTSLSMKSRRTAQSGNLATRPCVGTSQESLQEEVISLTDERQTLSIMVSLIPACHCCLMNTRRGCSLCQCEGLKKELEELQQLHEGSQRERAALQLQLQSCKAELQELLGRKLQVRGQKVSGSWVSLSMFSLSHLLMLVLRLFSAFIFSSPLLPLHPTLSSSQNCTRPSEPPVLSIPFIGLIVIVALIWCWWEELAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTA8</accession>
    <name>A0A3B5LTA8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">von Willebrand factor A domain containing 5A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTA8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011044"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011171.1">
        <property type="protein sequence ID" value="ENSXCOP00000011044.1"/>
        <property type="gene ID" value="ENSXCOG00000008328.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162662"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45737:SF6">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08487">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00609">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51468">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VIT" evidence="3">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="277"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="339"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="773" mass="84555" checksum="1C4D4C5DEF54C31C" modified="2018-12-05" version="1">MVHSCLSFADLVPLKSIDAQLEVKDHVATLVSTLNYENKEDKPLEAVFVFPLPGDAAVCHFSAQIGETHIVAEVKEKQQAQEEYDDALSSGQQAFLLEESEQSPDIFSLKVGRLPPGESASIRLEYVTELAVQADDGLRFCLPAVLNPRYQSQGSEGPSVQVTSVPASLVPYSLSFSAQVSSPRPISKIESSCSLEPLQYLNADQTQATIKLGGGHKFDRDIELLIYYKDAHQPSAVVEAGQASAKPGSLMGDPVVMVSLYPEFPQSVMSSLATCGEFVFLMDRSGSMGCSMNNSTPQETRISSARDTLLLLLKSLPMGCYFNIYSFGSSFEHIFPKSVEYSEKTMKEALEEVEKMESNLGGTEILQPLKHIYSQACIPKHPRQLFVFTDGEVGNTKEVTDLVKKNADSHRCFSFGIGEGASSALINGMAKDGGGHAQFITGADRMQPKVMQSLRFALQPAVEDISVSWDLPKGVSATVLSQPITAIFQGQRSLIYAQLTLQVSSEEADGTVTVKYSLAGHPSQSQLHFNLKPAEDSGLTVHRLAARTMIRSLESEERTQSEQNDEEMKKRVVELSVQSGVSSFFTAFIAVNKDSGESVEGPLVRRNIPTPFFPSESCATQCTHLSSSFHFTGSWLDMPALSAAEPPQPRKDPVLQLVSLQKASGCWMLDANLAAMLGKTSEQVEKAKPPSVVWASILALIWLHGFKMDVMEEWELLSMKAASWIKAQNGNIYNQQGSVTFTMMCLKQKVKLDAKIILVKSFLSFSSTCVRVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUN2</accession>
    <name>A0A3B5LUN2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Secreted</location>
            <location evidence="1 9">Extracellular space</location>
            <location evidence="1 9">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUN2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015703"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015897.1">
        <property type="protein sequence ID" value="ENSXCOP00000015703.1"/>
        <property type="gene ID" value="ENSXCOG00000011863.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157480"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048513">
        <property type="term" value="P:animal organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000001">
        <property type="entry name" value="Wnt homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF70">
        <property type="entry name" value="PROTEIN WNT-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 9" id="KW-0879">Wnt signaling pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="222" mass="25488" checksum="FE04D32422774C58" modified="2018-12-05" version="1">MAAGLVHSITRSCSQGNMTECWHWGGCSDHIRFGTWFSRRFMDVSEKNSSMDRVGFTLGTMNQHNAEAGRQAIETTMSTDCRCHGISGSCAVKTCWRSMAPFQRVGAFLKERYERSVQVSDRARRKTRKKDRRHLVDKNELIYLNKSPNYCLEDRRRGIAGTGGRRCNRTSTGPDGCNLLCCGRGYNTHVVRNIQRCECKFVWCCYVHCRRCESMNDMHTCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUY5</accession>
    <name>A0A3B5LUY5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUY5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011644"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011777.1">
        <property type="protein sequence ID" value="ENSXCOP00000011644.1"/>
        <property type="gene ID" value="ENSXCOG00000008793.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156897"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015616">
        <property type="term" value="F:DNA translocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045003">
        <property type="term" value="P:double-strand break repair via synthesis-dependent strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.850:FF:000002">
        <property type="entry name" value="DNA repair and recombination protein RAD54-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000332">
        <property type="entry name" value="DNA repair and recombination protein RAD54-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="97"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="341" mass="39036" checksum="82BBCD1867ED819F" modified="2018-12-05" version="1">MLNRTLIPLLYRLTPLQTELYMRFLKQAKPTETLREGRINVSSLSSITSLKKLCNHPALIYDKCVEEEEGFEGALHLFPPGYSAKVLEPQLSGKMVTLDYILAVTRSTTGDKVVLVSNYTQTLDLFEKLCRFRRYLYVRLDGTMSIKKRAKIVEKFNSPQNPEFIFMLSSKAGGCGLNLIGANRLVMFDPDWNPANDEQAMARVWRDGQKKTCYIYRLISTGTIEEKILQRQAHKKALSSCVVDEEQDVERHFSLGQLRELFTLNEETASDTHDKFHCCRCVNGREVRLPPEGADCTSDLSQWNHCFDKKGLRDQVLKASWNSAVSFVFHQRSHEDQKGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LV80</accession>
    <name>A0A3B5LV80_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CRIB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BORG/CEP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027856544.1">
        <property type="nucleotide sequence ID" value="XM_028000743.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027856546.1">
        <property type="nucleotide sequence ID" value="XM_028000745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LV80"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014961.1">
        <property type="protein sequence ID" value="ENSXCOP00000014775.1"/>
        <property type="gene ID" value="ENSXCOG00000011203.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114134267"/>
    <dbReference type="KEGG" id="xco:114134267"/>
    <dbReference type="GeneTree" id="ENSGT00940000167811"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030838">
        <property type="term" value="P:positive regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031274">
        <property type="term" value="P:positive regulation of pseudopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029273">
        <property type="entry name" value="Cdc42_effect-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051296">
        <property type="entry name" value="Cdc42_Effector_BORG/CEP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15344:SF20">
        <property type="entry name" value="CDC42 EFFECTOR PROTEIN 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15344">
        <property type="entry name" value="CDC42 EFFECTOR PROTEIN BORG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14957">
        <property type="entry name" value="BORG_CEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIB" evidence="4">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="76"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="199" mass="22469" checksum="DDF72A093E9A22BB" modified="2018-12-05" version="1">MPLKTSLYRNPSSSRWSSRNSKRREVLSVEMISLPLGDFRHVSHIGSDSQDDSFGDLSFLKMGHGLLRQSSKSEQNLFLSCKPPPKPPRLNLDGKEGMQSPEWSVDKKRKKCNSLPLLDSEEEDKCENEEVNETRNDFASNQTCRPGSGSVGSDRDREFTGSCEHIDEFKDEDSGFSFSLDLGPSILEDVLRVMDKLHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVX7</accession>
    <name>A0A3B5LVX7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVX7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011989"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012127.1">
        <property type="protein sequence ID" value="ENSXCOP00000011989.1"/>
        <property type="gene ID" value="ENSXCOG00000009075.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234557"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF18">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="6"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="179" mass="20180" checksum="C8FC4E9F81DE6C10" modified="2018-12-05" version="1">MFCSSPECKGDSVKQTEGEVVATAGESLTLRCTYETSVSVTLFWYKQEGNSFPKYMLKEYVKLPDNAPEFSKDRFDAKVNNSAFDLKIHNLHPSDSAVYYCALQPTVTGNTRTLHKNLQFSTAATRGPHSLDSEIHGFCLLIHCESGNSDFYSGNYGFKDRNESKCFNSICWAFRNVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LW47</accession>
    <name>A0A3B5LW47_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-terminal asparagine amidase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027873261.1">
        <property type="nucleotide sequence ID" value="XM_028017460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LW47"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016188"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016394.1">
        <property type="protein sequence ID" value="ENSXCOP00000016188.1"/>
        <property type="gene ID" value="ENSXCOG00000012236.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114144532"/>
    <dbReference type="CTD" id="123803"/>
    <dbReference type="GeneTree" id="ENSGT00390000016730"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008418">
        <property type="term" value="F:protein-N-terminal asparagine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026750">
        <property type="entry name" value="NTAN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12498">
        <property type="entry name" value="N-TERMINAL ASPARAGINE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12498:SF0">
        <property type="entry name" value="PROTEIN N-TERMINAL ASPARAGINE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14736">
        <property type="entry name" value="N_Asn_amidohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="34294" checksum="D82262763E47A5E9" modified="2018-12-05" version="1">MPLFFQNRELDCFRSTAELFDKYPHLQENAKAFRSKPRVDVDAKHLLYVQQREFAATTPADNCVSILGSDDATTCHLVVLRHTGSGAVCLSHLDGSSTRTEVQLTMKAVTSLSNGCNEGRFELHLVGGFDDDDKRSHKLSLNILAAFQKQKDHIHLETCCITEMNNVVDKGIHKPIVYGIGVNVKTGEVFPASFPHKGPAEMLRSARTFTGGEMTDIYDSRHGLIKVGPCQWSPDLEIAFWLSQSDATILKYLSTSPFAEPPHFVQHIKSTIQFLLSHPNSDSLFPGGQPHLYHWTESGSWERLVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWS2</accession>
    <name>A0A3B5LWS2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Collectin-12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">COLEC12</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWS2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015587"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015780.1">
        <property type="protein sequence ID" value="ENSXCOP00000015587.1"/>
        <property type="gene ID" value="ENSXCOG00000011589.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183074"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03590">
        <property type="entry name" value="CLECT_DC-SIGN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033989">
        <property type="entry name" value="CD209-like_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058762">
        <property type="entry name" value="COLEC12_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26004">
        <property type="entry name" value="COLEC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0176">Collagen</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0430">Lectin</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="18">
        <location>
            <begin position="595"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="429"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="162"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="509"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="531"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="736" mass="80130" checksum="D054129C4AF66EA4" modified="2018-12-05" version="1">MKHTKSCGIHCSHPGIQEGTQCTKCKNEWALKTSIALLYVLCTLLTIAVAVLGYKVVQRVDSVSEGIASYGGKIIAVETDLKKLDDQTEEKSENTTTEIQAFKSNIWALQRQLSAVQEHVHSDQVKLNQLQSIGSEIQSSQGTVRGRLSSNTATLHLVNSTLQSYGNIIESLQEDTARLQRELQQQVKLQSQALLSISNLNLTQAQQRGLIAALQRVVDETSQAIHKMRNDYQNLEQTARQTRSDAEWLRGKMENLQVIASNASTLAKANNDSLEDVGAQLAFMTSQLQNTSRLAEDHDQTLREIMDRQRDFSNLTSTKFDRVEMQVDELEETMDRVTGNMSHTTQLLGAINLNLNDLRSCSETIGRHSDVLQNLNDSVSDVRVDASSLRSQQEELAARLDKEVTSLSFVMEEMKLVDTKHSQLITNFTILQGPPGPRGPRGDRGPQGPPGQTGTKGERGEKGPLGIRGSRGEQGSPGPPGLPGLKGLPGVPGSPGPKGSRGSGGRAGPPGAKGEPGQAGLPGRDGQTGVPGLQGPPGIRGPIGPAGEQGPVGPPGLPGIPIQSPAVPFPPVSQQEEAVPHALLAPGCPPGWVNYKNRCYYFSKDLDSFDDSKANCESQLATLLIISDKEEQEWLEGQIVGKGFFWIGLTDWEEENVWRWVDGTIPAFTSWKPGQPDNWGHSHESGEDCAGLIHEGLWNDFFCEDLISYICEKELETCKSLSLLLAHLFLPLFPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXW2</accession>
    <name>A0A3B5LXW2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXW2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015745"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015939.1">
        <property type="protein sequence ID" value="ENSXCOP00000015745.1"/>
        <property type="gene ID" value="ENSXCOG00000011899.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000247201"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="140" mass="16504" checksum="6695B112630C45DB" modified="2018-12-05" version="1">ESSHRQMNPFGLGSFCFVELFKQQSEELYEQKYRKDTGYNHPQTRGHSRPKATRYSRPKATRYSRPKATRYSRPKATKYSRPKATRYSRPKATRYSRPKATRYSRPKAMKYSRPKGDEGCYLGIRLTVVSPLLAQTHQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYR6</accession>
    <name>A0A3B5LYR6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Myosin binding protein C, cardiac</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYR6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014486"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014671.1">
        <property type="protein sequence ID" value="ENSXCOP00000014486.1"/>
        <property type="gene ID" value="ENSXCOG00000010987.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157698"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032036">
        <property type="term" value="F:myosin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055010">
        <property type="term" value="P:ventricular cardiac muscle tissue morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000225">
        <property type="entry name" value="Myosin-binding protein C, cardiac-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000060">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF20">
        <property type="entry name" value="MYOSIN-BINDING PROTEIN C, CARDIAC-TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="93"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="328"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="430" mass="47964" checksum="222451C0A8655052" modified="2018-12-05" version="1">MIKLQLPKVAEKKPVEDLMLVEAEEETNRAKSSLTPAARRLSPTAPPAELAFSLEVGDPADNEKPAGGSASFPAGKETALYLPSAALSAPTSEPIGLHVEDISDTSITLKWRAPERIGAAKLEGYGVEYCKEGTDEWIPANQGLTERSSMTIKNLTTGDKLLFRVRAYNMAGPSSPAVLAQPVTIREIMQRPKIWLPRNLRQTLIKKVGETVNIMIPNDEPVGKDLLLPPSSTSSLMFWTYLVLSNRIEAFYVLHIKEWFTVHDHYRRTHCVASDLIMGNEYIFRVFSINLVGLSLEPLTTKDSVFIQKSGIDYKSPAYREHDFSEAPKFTHPLVNRSIIAGYNATLSCAVRGIPKPKVTWYKNKMDISNEAKYRMLSKQGILTLEIRKPCPFDGGVYMCKAVNDKGEDVVECKLEVRPQIAKEEKKEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYW4</accession>
    <name>A0A3B5LYW4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fork-head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYW4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013129.1">
        <property type="protein sequence ID" value="ENSXCOP00000012969.1"/>
        <property type="gene ID" value="ENSXCOG00000009825.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160522"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20043">
        <property type="entry name" value="FH_FOXB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000082">
        <property type="entry name" value="forkhead box protein B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047389">
        <property type="entry name" value="FOXB1_B2_FH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF209">
        <property type="entry name" value="FORKHEAD BOX PROTEIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="13"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="13"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="267"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="269"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="299" mass="32614" checksum="3B34BC144C904300" modified="2018-12-05" version="1">MPRPGRNTYSDQKPPYSYISLTAMAIQSCPDKMLPLSDIYKFIMERFPYYRENTQRWQNSLRHNLSFNDCFIKIPRRPDQPGKGSFWALHPNCGDMFENGSFLRRRKRFKVGGVPVPDPLCPSKPQDAAHYLQQQAKLRLSALHAAAHLPQVPAGYGLSSTQPSSFKHPFAIENIIAREYKVPGGLAAFPAAGYPLHNQLSPAWPHMYGPGVMDPATPLSVAPADYSAYGMPLKTLCHGGQTLPAIPVPIKPAPGLPAHIPAFLAHSPRSLSPTSPQTAAGQSSPAGPAEPLQSAVAVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZ35</accession>
    <name>A0A3B5LZ35_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL14m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L14, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZ35"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013034"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013194.1">
        <property type="protein sequence ID" value="ENSXCOP00000013034.1"/>
        <property type="gene ID" value="ENSXCOG00000009872.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001121"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000004">
        <property type="entry name" value="39S ribosomal protein L14, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21037">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L14, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21037:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="147" mass="16420" checksum="751F527056F36309" modified="2018-12-05" version="1">MKVFHGFQKLSKFWRQITDLIFYLSSVSIATAAIQKMTRVRVVDNSSLGNTPYHRPPRVIHVYTKNGIGKVGDKVLLAIKGQKKKALIVGHKMPGDRMTPRFDSNNVVLIEDNGNPTGTRIKVPIPSSLRQMEGNYSKVLAIANTFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZC5</accession>
    <name>A0A3B5LZC5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <ecNumber evidence="5">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">40S ribosomal protein S3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="5"/>
    <dbReference type="RefSeq" id="XP_027900357.1">
        <property type="nucleotide sequence ID" value="XM_028044556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZC5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016517"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016724.1">
        <property type="protein sequence ID" value="ENSXCOP00000016517.1"/>
        <property type="gene ID" value="ENSXCOG00000012468.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114161336"/>
    <dbReference type="GeneTree" id="ENSGT00390000008610"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001235">
        <property type="term" value="P:positive regulation of apoptotic signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02413">
        <property type="entry name" value="KH-II_40S_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1140.32:FF:000005">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000006">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KH_dom-like_a/b"/>
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    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
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    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_uS3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_uS3_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003219">
        <property type="entry name" value="PRK04191.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
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    </dbReference>
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    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
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    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
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    <keyword evidence="10 17" id="KW-0689">Ribosomal protein</keyword>
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        <location>
            <begin position="21"/>
            <end position="92"/>
        </location>
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        <location>
            <begin position="200"/>
            <end position="245"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
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            <dbReference type="ARBA" id="ARBA00004604"/>
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            <dbReference type="ARBA" id="ARBA00010761"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00044632"/>
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    </evidence>
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            <dbReference type="RuleBase" id="RU003624"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Ensembl" id="ENSXCOP00000016517.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="245" mass="26851" checksum="3CAD13A3C911A23A" modified="2018-12-05" version="1">MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPSGKIGPKKPLPDHVSIVEPKEENLPTTPTSEQKGAKPEVPVMPQGVAVPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M1F2</accession>
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        <recommendedName>
            <fullName evidence="1">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="entry name" value="C_TYPE_LECTIN_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="61"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="164" mass="18363" checksum="5506E85B6F1A1675" modified="2018-12-05" version="1">MSPRATSASFCIIICSLVAIVTKCLTPPFWITVLTTSYNPKTEVRSIAVKYPRPISSVFLAQSYCRTHHTDLAMIENEAENSAVKQLIPVGVNVWIGLYRVPWSWSDKRPSLFRRWVSPEPDNYGGIQNCVCENTAHMWGDEKCSATKAVCFCLNVVVPTVTSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4Q8</accession>
    <name>A0A3B5M4Q8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">LCCL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4Q8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014954"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015142.1">
        <property type="protein sequence ID" value="ENSXCOP00000014954.1"/>
        <property type="gene ID" value="ENSXCOG00000011336.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Cysteine-rich secretory protein LCCL domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.130.20:FF:000001">
        <property type="entry name" value="Cysteine-rich secretory protein LCCL domain-containing 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.20">
        <property type="entry name" value="LCCL-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051957">
        <property type="entry name" value="CRISP-LCCL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004043">
        <property type="entry name" value="LCCL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036609">
        <property type="entry name" value="LCCL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31331:SF1">
        <property type="entry name" value="CYSTEINE RICH SECRETORY PROTEIN LCCL DOMAIN CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31331">
        <property type="entry name" value="LCCL DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G08630)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03815">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00603">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69848">
        <property type="entry name" value="LCCL domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50820">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017398897" description="LCCL domain-containing protein" evidence="7">
        <location>
            <begin position="25"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="LCCL" evidence="8">
        <location>
            <begin position="283"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="LCCL" evidence="8">
        <location>
            <begin position="378"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="252"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="252"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="486" mass="54607" checksum="C8C061EF41E745A7" modified="2018-12-05" version="1">MTHSASPCLLFLSLLMRCVTDSSSLFLPDSKELRHLLSRYEQEADHNGVGSTAGNRTRRAILWSDREEILQLHNKLRGSVYPTASNMEYMVWDDELERSATHWAEECQWDHGPQDLLMSIGQNLAVHWGRYRSPAFHVQAWYDEVKDYTFPYPQECNPWCPDRCSGPMCTHYTQLVWATTNRVGCAVHVCPRMNVWGEIWENAVYLVCNYSPKGNWIGEAPYQHGRPCSQCPPSFGGGCRNNLCYKDSQRSETEDMNEVEKPQVPLSPRTTSKPTPRPPRKPCETKLRDKCKGATCNRHNCPANCLNTKGKVWGTVSYDVQSSICQAAIHSGVIDNNGGLVDITRTDKLPFFVRSTKNGVESLSKYKPGNSFTVAKVEEYNADCYTTVREICPYRQPFSHCPRIICPASCKTQPSYWSPVVGNNIYADSSSICKSAIHSGVIKGDGGFVDVLPLERRKSYAGVLKNGIQSESNTDGGSFRLFAVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4U6</accession>
    <name>A0A3B5M4U6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cytochrome P450, family 17, subfamily A, polypeptide 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027863355.1">
        <property type="nucleotide sequence ID" value="XM_028007554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4U6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018395"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018627.1">
        <property type="protein sequence ID" value="ENSXCOP00000018395.1"/>
        <property type="gene ID" value="ENSXCOG00000013854.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114138338"/>
    <dbReference type="KEGG" id="xco:114138338"/>
    <dbReference type="CTD" id="1586"/>
    <dbReference type="GeneTree" id="ENSGT00940000155588"/>
    <dbReference type="UniPathway" id="UPA00062"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047006">
        <property type="term" value="F:17-alpha,20-alpha-dihydroxypregn-4-en-3-one dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004508">
        <property type="term" value="F:steroid 17-alpha-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903449">
        <property type="term" value="P:androst-4-ene-3,17-dione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030237">
        <property type="term" value="P:female sex determination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042446">
        <property type="term" value="P:hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030238">
        <property type="term" value="P:male sex determination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042448">
        <property type="term" value="P:progesterone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd20673">
        <property type="entry name" value="CYP17A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000002">
        <property type="entry name" value="Cytochrome P450 1A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289:SF14">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 17, SUBFAMILY A, POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24289">
        <property type="entry name" value="STEROID 17-ALPHA-HYDROXYLASE/17,20 LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017176957" evidence="12">
        <location>
            <begin position="19"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="514" mass="57339" checksum="4E8D63CAE1072843" modified="2018-12-05" version="1">MACFFLIVFAILVLVVLTWQLKVKSSFGSQEPPHLPALPLIGSLLSLRSQHPPHVLLKELQKKYGQTYSLMMGSHRVVIVNQHVHAKEILLKKGKIFAGRPRTVTTDILTRDGKDIAFGDYSPAWRFHRKIVHGALCMFGEGSATIERMICAEAQSLCSTVSEAATLGLALDLAPELTRAVTNVVCSLCFNSSYARGDPEFEGMLHYSQGIVDTVAKDSLVDIFPWLQIFPNADLRLLKHCVSVRDKLLQKKYDEHKADYSDNVQRDLLDALLRAKRSAENNNTADVDAATAGLSDDHLLMTVGDIFGAGVETTTTVLKWAVTYLIHHPDVQRRIQDELDTKIGADRSPRLRDRGSLPYLEATIREVLRIRPVAPLFIPHVALSDTSIGDFKVKKGTRVIINLWSLHHDEKEWSHPEHFDPGRFLDSEGTGLTIPSSSYLPFGTGIRVCLGEALAKMELFLFLSWILQRFTLSVPPGHPLPSLEGKFGVVLQPVKYKVKATPRPGWERAKSQMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4V4</accession>
    <name>A0A3B5M4V4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Leydig cell tumor 10 kDa protein homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0390 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027873563.1">
        <property type="nucleotide sequence ID" value="XM_028017762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4V4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019198"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019435.1">
        <property type="protein sequence ID" value="ENSXCOP00000019198.1"/>
        <property type="gene ID" value="ENSXCOG00000014433.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114144683"/>
    <dbReference type="KEGG" id="xco:114144683"/>
    <dbReference type="CTD" id="101102725"/>
    <dbReference type="GeneTree" id="ENSGT00390000011740"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019034">
        <property type="entry name" value="UPF0390"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16967">
        <property type="entry name" value="LEYDIG CELL TUMOR 10 KDA PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16967:SF1">
        <property type="entry name" value="LEYDIG CELL TUMOR 10 KDA PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09495">
        <property type="entry name" value="DUF2462"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="63"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="97" mass="10359" checksum="8B5CD0A12E902A06" modified="2018-12-05" version="1">MAQGSKKFKAQRPGGSKKHQQNKQKGPKKGGRIIAPKKAQVVQQQKLKKGLEVAIRNKIEQEVTQKASSSLHKPLAVVKGAEGKAKPAAARPGTSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5W2</accession>
    <name>A0A3B5M5W2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M5W2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016801"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017018.1">
        <property type="protein sequence ID" value="ENSXCOP00000016801.1"/>
        <property type="gene ID" value="ENSXCOG00000012676.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155604"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058770">
        <property type="entry name" value="PWI_ABCF3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF117">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26051">
        <property type="entry name" value="PWI_ABCF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABCF3 PWI-like helical bundle" evidence="4">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="166"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="103"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="103"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF26051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="312" mass="34995" checksum="1D3AED7385EEEEE1" modified="2018-12-05" version="1">MATYVDILKREFPEIDTELFDYITGVLDSGASDFEDGEEVFDAIGGVLQDVSADSKNEDDVRDIYPSMNNHHHPQKQMLLDAPVQLSQMSTVDAKKLEKAEAKLKAKNERRTERDTQKATGPVLEEASASQASNKKESRADQSGKNRSYDIRIENFDISFGERCLLQGAELSLASGRRYGLIGRNGLGKTTLLKMLASRNLRVPAHISILHVEQEVAGDDTIALQSVLESDTKREDLLNEERRACDCLRSTPNWRRSKPTRPRHGNYGNTLVAQRMVLERFFILSASYRPIRASVILAGLGFSPRMQQQPTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M728</accession>
    <name>A0A3B5M728_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PABIR family member 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM122 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M728"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015802.1">
        <property type="protein sequence ID" value="ENSXCOP00000015609.1"/>
        <property type="gene ID" value="ENSXCOG00000011796.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015476"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026716">
        <property type="entry name" value="PBIR1/2/3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22227">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 122B ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22227:SF6">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 122B ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="115"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="234"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="234"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="255"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="273" mass="29884" checksum="ED34CBF8667CDDC7" modified="2018-12-05" version="1">MTQEKMELDLEIPAALVQSDGHLRRSNSAPMINGLSDNAQVFQREILRCRRNSTTVVNRPSMVPSSPIRVPSTRLHQIKQEEGVDLMNRETAHERGVQVAMQMSHSWEESLSLSDNDMEKSSSPKRIDFVPVSPAPSPTRGIGKKQCFSPSLQILVSSNGLTPSPIPSPTRRFSRRSQSPINCIRASILGPMKRKGEMETESQPKRLFQGTTTMLSSDMSNLSDLSSCHSPDLLDGSLSSIGSSTDSPGKMEGVFPSSSSNSPFTSLQDLSPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8J5</accession>
    <name>A0A3B5M8J5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RAB4a, member RAS oncogene family</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M8J5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016094"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016297.1">
        <property type="protein sequence ID" value="ENSXCOP00000016094.1"/>
        <property type="gene ID" value="ENSXCOG00000012162.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157464"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001944">
        <property type="term" value="P:vasculature development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004198">
        <property type="entry name" value="Secretion related GTPase SrgD (AFU_orthologue AFUA_1G07680)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="187" mass="20750" checksum="CE21975F64E35A4A" modified="2018-12-05" version="1">SMSASLVKDESNHTIGVEFGSKIISVVNKTVKLQIWDTAGQERFRSVTRSYYRGAAGALLVYDITSRETYNALTTWLSDARMLASQNIVIILCGNKKDLDADREVTFLEASRFAQENELMFLETSALTGENVEEAFVQCARKILNKIESGELDPERMGSGIQYGDAALRQLRSPRRAQPQGSQDCGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8Z1</accession>
    <name>A0A3B5M8Z1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thyroglobulin type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M8Z1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017965.1">
        <property type="protein sequence ID" value="ENSXCOP00000017741.1"/>
        <property type="gene ID" value="ENSXCOG00000013368.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000169140"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
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    <sequence length="87" mass="10635" checksum="03A0DF6A4E10FB45" modified="2018-12-05" version="1">MQSEEGTEWLLELLTDVQLQQYFLRIRDELNVTRLSHFDYVKNEDLEKIGMGRPGQRRLWEAVKRRKALYKRKSWMSKEGHKIPFPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAC9</accession>
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        <scope>IDENTIFICATION</scope>
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    </evidence>
    <sequence length="736" mass="81469" checksum="5EA9443EF2911E0E" modified="2018-12-05" version="1">MVEQAEIRVVHQGGSNITFREPAISSDSRFLLCASGHNVKVFSTVTEECVQELRGHTDLVTGVLIRPSNHLQAYSCSADGTVRLWDFTDGILIKVKTFPPSSEGSIRLIKPSDSLQLLAVHLPQTAGQLVETSDLSAVLSDVSPNPATVAFGRALMIFYDKLSIFCCCASRFSLKENSCSGRSNTFTCVACHPTDDCVATGHEDGKIRLWRNFDQKREYTYRTLHWHHGAVRALRFTPEGSNLLSGGVESVLVQWQYQQESQQDFLPRLGAAITHIAVSPDGALFCTSHSDNKITVIQSCVKVSSIIQGLVKGEAVQTDLMVDPRSKALVLNGKPGHLQFYSLHRDRLLFNLDVVQQEFIHQEGLDQFQVVMATLDGAGAWLATVEERKQETAEAELNLKLWAFDEPSQSFVLNTTVSTPHEARITAVTFDPAARGQTSLLVSCGQDGLFKAWGLTVPSPDGGPSWSCDFVSAYHGLAPQRCCFSADGSLLAVGFQEVVTVWSPASWELLLTLSQPPGNIRDLCFSRLSCSKFLLGTTSCNQLCCWNLLTCSLEWSTSMEVSLLLADPLSDNMAAFCHQDGNTDLFVFKPSEPRPLFSQKAVCSGRVSRAVFAPREVALESCEESSRWLNRSQLYFLTPLMVNKWAKYRPISSKLTLWERGPTLTEKKTSAGETPSDMEEEEEEDSDEEMETRATRSEEAPLETDSAGPVLSRAQVRELRRVRKLDHSWFSSLLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MF53</accession>
    <name>A0A3B5MF53_9TELE</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MF53"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019821"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020066.1">
        <property type="protein sequence ID" value="ENSXCOP00000019821.1"/>
        <property type="gene ID" value="ENSXCOG00000014876.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="111" mass="12668" checksum="165F11193D633D8A" modified="2025-10-08" version="2">MKDLVKRLSTTPIFFFLVNAHQLKLLWNSILWESFSSAVTGKLIRSDRKMDKIQYGAILKENQLGARDTMEWFRSNHLCVLEGGSQSSELQPTQTLTAPNSKLPLQTFLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJ88</accession>
    <name>A0A3B5MJ88_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CARD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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        <property type="component" value="Unplaced"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="51" mass="6072" checksum="0905CF92419EC6A5" modified="2018-12-05" version="1">TQFVREMNETHRDKARVLIDTVRRKGDDASSEMIHFLCELDHRFSEHLGLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MK39</accession>
    <name>A0A3B5MK39_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Osteoclast-stimulating factor 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Induces bone resorption, acting probably through a signaling cascade which results in the secretion of factor(s) enhancing osteoclast formation and activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Lamellipodium</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MK39"/>
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    <dbReference type="Ensembl" id="ENSXCOT00000021754.1">
        <property type="protein sequence ID" value="ENSXCOP00000021491.1"/>
        <property type="gene ID" value="ENSXCOG00000016088.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030032">
        <property type="term" value="P:lamellipodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="PH_Cool_Pix"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000016">
        <property type="entry name" value="Rho guanine nucleotide exchange factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.390">
        <property type="entry name" value="L1 transposable element, trimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CH_dom"/>
    </dbReference>
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        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="DH_dom"/>
    </dbReference>
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        <property type="entry name" value="GEF6/7_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046376">
        <property type="entry name" value="PH_Cool_Pix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
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    <dbReference type="PANTHER" id="PTHR46026:SF2">
        <property type="entry name" value="RHO GUANINE NUCLEOTIDE EXCHANGE FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RHO-TYPE GUANINE NUCLEOTIDE EXCHANGE FACTOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="betaPIX_CC"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RhoGEF67_u1"/>
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    </dbReference>
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        <property type="entry name" value="RhoGEF67_u2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="14">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="11">
        <location>
            <begin position="159"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="DH" evidence="13">
        <location>
            <begin position="230"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="12">
        <location>
            <begin position="429"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="117"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="531"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="597"/>
            <end position="623"/>
        </location>
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        <location>
            <begin position="712"/>
            <end position="739"/>
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        <location>
            <begin position="118"/>
            <end position="131"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037432"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50010"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="745" mass="83609" checksum="F3E881C66FC894A4" modified="2018-12-05" version="1">MNPEEQTVTWLISLGVLSSPKKNIADPEEFLKTSLKDGVVLCKLTERLIPGFTPKYSQDPRTEADCISNIKEFLRGCSSLKVEGFEPEWLYTGENFGKVLNTLLAFNFATQCAVERCPQSGSSSPNQTTSSHALSSTRSKGSLRRQSKSVQEMSENGGGGQVMVKARFNFKQNNEDELSFSKGDMIIVTRQEDGGWWEGTLNSKTGWFPSNYVREIKPCKPLSPKGTPLTKNYFSVDILEHEREFVKELQTVLTCYLRPLQANDDSATLCGNLEEILTFQQGLCLALEDCTVAEGQQRVAGCYLNLMSQIKSLYLTYCSSHPSAVCILTDHEELGKFIESQGASTSGILTLTTSLSKPFIRLDKYPTLLQELERHVEEAHPDYADIIKATAAFKSLVCQDLRKRKNLELQILSEPVRGWEGDSMKSLGQVAYMSLVHVRNGASEEKEERYLMLFPNVLVMLSASPRMSGFIYQGKLSLTGTTVTRQVEDGENTPHAFDITGMIDRFSVFCSSAQELQEWLEHLQPYTKTANGKPLSAVGTPTHLPHLSGPSAVSRGPLEPPKTSKPWTLSCLRPAPPLKPSAALGYKERMSYIMKLQDSSKSPRPIKKFLPGNRKKDRKPSDDEFQIRKTAALEEDAQILRVIEAYCTGASLHQTTTVRKECVPQVLLPEEEKIIVEEIKSNGQTVTEESLVDAVYALKDEVHELKKENKWMKQFLEEEQKSRKELERLVRKLSKQKNDCGWEDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLC3</accession>
    <name>A0A3B5MLC3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Vesicular glutamate transporter 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="28">Solute carrier family 17 member 7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="33 34">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>3 Na(+)(out) + phosphate(out) = 3 Na(+)(in) + phosphate(in)</text>
            <dbReference type="Rhea" id="RHEA:71255"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>L-glutamate(out) = L-glutamate(in)</text>
            <dbReference type="Rhea" id="RHEA:66336"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>phosphate(in) = phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:32823"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Synaptic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Synapse</location>
            <location evidence="22">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="26">Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLC3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024375"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024668.1">
        <property type="protein sequence ID" value="ENSXCOP00000024375.1"/>
        <property type="gene ID" value="ENSXCOG00000018203.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159110"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060076">
        <property type="term" value="C:excitatory synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005326">
        <property type="term" value="F:neurotransmitter transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098700">
        <property type="term" value="P:neurotransmitter loading into synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050803">
        <property type="term" value="P:regulation of synapse structure or activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007600">
        <property type="term" value="P:sensory perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035249">
        <property type="term" value="P:synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17382">
        <property type="entry name" value="MFS_SLC17A6_7_8_VGluT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000004">
        <property type="entry name" value="vesicular glutamate transporter 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000005">
        <property type="entry name" value="vesicular glutamate transporter 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF29">
        <property type="entry name" value="VESICULAR GLUTAMATE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0868">Chloride</keyword>
    <keyword evidence="16" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="20" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="19" id="KW-0407">Ion channel</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 31" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="6" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0915">Sodium</keyword>
    <keyword evidence="17" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="10" id="KW-0769">Symport</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="7" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="8 31" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 31" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="33"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="176"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="349"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="32">
        <location>
            <begin position="32"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="509"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="533"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="30">
        <location>
            <begin position="510"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00035839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00036616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00036683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00038044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00039390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00041351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00047912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="554" mass="60611" checksum="F7D1C711FF37AF14" modified="2018-12-05" version="1">ETIELSAEGRPELVEEKELPVVDCTCFGLPRRYIIAILCGLGFCISFGIRCNLGVAIVSMVNDHTVYKGNKEVLVAAQFTWDPETVGMIHGSFFWGYIVTQIPGGFICQKFAANRVFGFAIVATSVLNMLIPSAARCHYSCVILVRICQGLVEGVSYPACHGIWAKWAPPLERSRLATTAFCGSYAGAVVAMPLAGILVQYIGWPSVFYVYGSFGIFWYLFWVLVSYESPAVHPTITPEERKYIEEAIGESAAFLNPLHKFKTPWRNFFTSMPVYAIIVANFCRSWTFYLLLISQPAYFEEVFGFEISKVGIVSALPHLVMTIIVPIGGQLADYLRTHNLMSTTNVRKLMNCGGFGMEATLLLVVGFSHTKVIAITFLVLAVGFSGFAISGFNVNHLDIAPRYASILMGISNGVGTLSGMVCPLIVGAMTKHKTREEWQYVFLIASVVHYGGVIFYGIFASGEKQWWADIEDTSEEKCGIINEDELANETEELYRGGGQYGAMGQPVVGSNGGGGGGGGGGAGWVTDWDKSEEYVQPPGYNSYMHSGGKEKELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLI7</accession>
    <name>A0A3B5MLI7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CKLF-like MARVEL transmembrane domain containing 6</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLI7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025569.1">
        <property type="protein sequence ID" value="ENSXCOP00000025264.1"/>
        <property type="gene ID" value="ENSXCOG00000018869.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000177034"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098797">
        <property type="term" value="C:plasma membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021663">
        <property type="entry name" value="CD3_zeta/IgE_Fc_rcpt_gamma"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11628">
        <property type="entry name" value="TCR_zetazeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017273522" description="CKLF-like MARVEL transmembrane domain containing 6" evidence="8">
        <location>
            <begin position="18"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="70"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="11417" checksum="78E8FEE2E9C3C7A6" modified="2018-12-05" version="1">MWSRVTTCLCFLFVSSAETFFTDPVICYFLDAFLMVYCIAATALFFREKVSFSKGGPHFRADEVKGRTCVSGDMKGERGEEEVLEKERDAMEESKGGRTKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQX2</accession>
    <name>A0A3B5MQX2_9TELE</name>
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        <submittedName>
            <fullName evidence="2">Eosinophil peroxidase</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Atherinomorphae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <dbReference type="STRING" id="32473.ENSXCOP00000026814"/>
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        <property type="gene ID" value="ENSXCOG00000020022.1"/>
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        <property type="entry name" value="Eosinophil peroxidase"/>
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        <property type="entry name" value="Haem peroxidase domain superfamily, animal type"/>
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    <dbReference type="InterPro" id="IPR037120">
        <property type="entry name" value="Haem_peroxidase_sf_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475:SF63">
        <property type="entry name" value="EOSINOPHIL PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03098">
        <property type="entry name" value="An_peroxidase"/>
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    <dbReference type="PRINTS" id="PR00457">
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    </dbReference>
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        <property type="entry name" value="PEROXIDASE_3"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
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        <source>
            <dbReference type="PIRSR" id="PIRSR619791-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="396" mass="44414" checksum="E5E6CFE2EDF1D7F7" modified="2018-12-05" version="1">IPLSDPRSGVQSCMPFFRSAPSCDNGVLPPRQREQLNAITSFVDASMVYGSSPGLASALRNQSSPLGSMALNSRYWDEELPYMPFLSRVQAHLDPCGPRNSSTVWASSRSALRENTTSCFHADSRVNEHLGLIVLHTLFLREHNRLVKELHQLNPHWSPETLYQEARKIIGAIHQILTWEHYLPRVLGESAMSQLMPRYQRYDPDVDPSIANTFATAAFRFAHVTVQPVVNRLGPDYTFNPEYPPLPLHHSLFASWRVIQEGIDPVLRGMLLSPAKLQTAGQMMVEELTERLFQAQGGMPLDLGALNLQRSRDHGLPYSSWRQFCNLSVPNTMSDLAEILGNFTLAHKFELLYGTPHNIDVWVGAISEPALPGGRVGPLLSCLLTRQFRALRDGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRL2</accession>
    <name>A0A3B5MRL2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
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            <name>Mg(2+)</name>
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        </cofactor>
    </comment>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000141">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF3">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="13">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="345"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="95"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="154"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="158"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="188"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="291"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="305"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="619" mass="68955" checksum="5299940D7B5799F6" modified="2018-12-05" version="1">MFRRRKKKYRLEISAPRDFQHRVHTSFDVSTGRYVGLPAQWQSVIDTLRRPRPLVDPSRITNIDLEPRKQTIVRGSFIGHGDYISHVISEMSRVSVTSSNSLRRTSPSARKRAQSMGRLGEVAEDESYHYLEISRRSSSVKAGGSATYWQDRIRQVRSESGSPRANRNLQRTKSSSQVSSVSEAAPLSPTSPQRTTYTPEPSVRNGGVGLKQRPTSYHQAAETGRPQLRPRAGANHLPDLLSSSTETPRRPHSTYDLKVRPGPSTSSPVITGRVLPQPSPRPSTGVDVSTKRASTPPQTSLAQPSRPPSSTPPAAPDRAPQKVTHEQFKAALEMVVDPGDPRVTLENFMKIGEGSTGVVCIARERHSGRQVAVKMMDVRKQQRRELLFNEVVIMRDYQHPNVVQMFRSALVGDELWVIMEYLQGGALTHIIGETRLNEEQIATVCEGVLQALSYLHSQGVIHRDIKSDSILLTLDGRIKLSDFGFCAQINTDVPKRKSLVGTPYWMAPEVISKTPYGTEVDIWSLGIMVVEMVDGEPPYFNETPISAMKKLRDEAAPSVKNMQRVSPVLKDFLGCMLTRDTLQRSGAAALLQHPFMLQAAAPRCLVPLVEQHRKRMSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRP5</accession>
    <name>A0A3B5MRP5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tubulin polymerization promoting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TPPP</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPPP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRP5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026453"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026774.1">
        <property type="protein sequence ID" value="ENSXCOP00000026453.1"/>
        <property type="gene ID" value="ENSXCOG00000019753.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153875"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001578">
        <property type="term" value="P:microtubule bundle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032273">
        <property type="term" value="P:positive regulation of protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000057">
        <property type="entry name" value="Tubulin polymerization-promoting protein family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008907">
        <property type="entry name" value="TPP/p25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12932">
        <property type="entry name" value="P25 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12932:SF18">
        <property type="entry name" value="TUBULIN POLYMERIZATION-PROMOTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05517">
        <property type="entry name" value="p25-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="187" mass="20811" checksum="398E1B72144253F6" modified="2018-12-05" version="1">MPENLGRTFFITALEESFRRFAIHGDTRATGKELHGKNWSKLCKDCGVIDGKNITLTDVDIVFSKVKKKSCRTITFEEFKVALGELARKKYKEKTGEDAEAEVFKLIEGKAPVITGVTRAVASPTVSRLTDTTKFTGSHKERFDETGRGKGKAGRVDLVDTSGYVSGYKHRGTYEKKVNKPTEGRPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSX7</accession>
    <name>A0A3B5MSX7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSX7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022604"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022880.1">
        <property type="protein sequence ID" value="ENSXCOP00000022604.1"/>
        <property type="gene ID" value="ENSXCOG00000016894.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163630"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd01650">
        <property type="entry name" value="RT_nLTR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31635:SF196">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="84"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="337" mass="38827" checksum="D8684E7013C68673" modified="2018-12-05" version="1">QKLYALKEQEGTDEANVLLNELLLPTVSNEQNEALTKNITPQEIEKAINKLKLIKLPRPDGFRAEWYKIYLKELSPVLLRTFNWVLNGGQIPPSWGETMITVIPKEGKDKLNCSNYRPISLLNQDYKIFTAILAKRLEHIPPDIINLDQTGFIKKIRTRDNIKRTLNVINHIKKTDKQAIIMGLDAQKAFDTVDWSFLFRVLSQFNFSSKFIKIIQELYNKPTGKIRINGGLSEEIKIEQGCRQGCSMSPLLFAIFIEPLAQWIRQSTKIHGIKLNHNEQKLALFADDILIYLVLTFKYSPDNIDKENFKINWDMDSITHIAPWTSSSKRVLSENTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MT60</accession>
    <name>A0A3B5MT60_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Efficient DNA binding requires dimerization with another bHLH protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MT60"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027594"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027929.1">
        <property type="protein sequence ID" value="ENSXCOP00000027594.1"/>
        <property type="gene ID" value="ENSXCOG00000020596.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165300"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021575">
        <property type="term" value="P:hindbrain morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050767">
        <property type="term" value="P:regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19742">
        <property type="entry name" value="bHLH_TS_ASCL1_Mash1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000029">
        <property type="entry name" value="Achaete-scute family bHLH transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015660">
        <property type="entry name" value="MASH1/Ascl1a-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935:SF133">
        <property type="entry name" value="ACHAETE-SCUTE HOMOLOG 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13935">
        <property type="entry name" value="ACHAETE-SCUTE TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="62"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="138"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="138"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="179" mass="20683" checksum="AE6F69BA70734A33" modified="2018-12-05" version="1">WIQKAFQRTKLCTFSFYSPLFFFFLKTKVLKRQRSSSPELLRCKRRLSFNGLGYSIPQQQPVAVARRNERERNRVKQVNMGFQTLRQHVPNGAANKKMSKVETLRSAVEYIRALQQLLDEHDAVSAAFQCGLPSPTLSNSYSADPESPHSTYSSDEGGYEPLSSEEQELLDFTTWFDRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MU21</accession>
    <name>A0A3B5MU21_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polysaccharide biosynthesis domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Neopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <feature type="domain" description="Polysaccharide biosynthesis" evidence="1">
        <location>
            <begin position="35"/>
            <end position="158"/>
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    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04669"/>
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            <dbReference type="Proteomes" id="UP000261380"/>
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    </evidence>
    <sequence length="166" mass="18989" checksum="840CBFE9DA819D8D" modified="2018-12-05" version="1">VCVCVCVCFQGVEGASAAAHALSLPAEAYGNDPRLEAMWAMKAYNHAEVYFNLISSVDPKFLKLTKVDDQIYSSFRETFKDLDVKRLKEEDLKSDEAKQTWRPFCNQFEGIVEDFNYGTLLRLDCEKDYSEDNTIFATRVQFFAVEIARNREGHNDTVFTSKRAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUH4</accession>
    <name>A0A3B5MUH4_9TELE</name>
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        <submittedName>
            <fullName evidence="11">Shox homeobox</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
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        <scope>IDENTIFICATION</scope>
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        <location>
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        </location>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
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    <sequence length="286" mass="32203" checksum="DE34235D7C14BC2D" modified="2018-12-05" version="1">MEELTAFVSKSFDQKSKESSKESITYREVLESGLTRARELGSPETNLQDITDTNHCPVHLYKDHVELEKEKLKDFNVSRCKEKKDDVKSEDEDAQGKLKQRRSRTNFTLEQLNELERLFDETHYPDAFMREELSQRLGLSEARVQVWFQNRRAKCRKQENQMHKGVILGSGSHLDACRVAPYVNMGALRMPFQQEIRRGLVAGLEVQAQLQLDGVTHSHPHLHPHLAAHAPYLMFPPPPFGLPIASLADSASAAAAVAAAAKSNSKNSSIADLRLKARKHTEALGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUH8</accession>
    <name>A0A3B5MUH8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">P2Y receptor family member 8</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A3B5MUH8"/>
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        <property type="project" value="InterPro"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="3 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="26"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="41"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="97"/>
            <end position="177"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR603912-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="334" mass="37829" checksum="AEBC297E94BDFC17" modified="2018-12-05" version="1">MKGNTSRTQLDNATLTLFRDVNTSTIISVIYVLVTIINWVGNGLSMWLLLFRTSPKTPSIIFMINLTLTDLALGSALPFQIAYQLQGYNWKLGSNMCSFMTLVFYTNMYCSILTMMAIGIDRYLGIVKPMRFRKNKRRKSIAVFSCLLMWGLVLGVLYPLMTTDLTFEVPELQITTCFDVLKKSMLPSVGAWATFLFSMVVFLFLFPFCVTMFCYVSVIHKLSSDSKTVQKGRAIRLAVVVLLVFVLCFAPNNILLLVHSVLRLFYDKSVYMAYKLSLCFSCLNSCLDPFIYYFASKDFRQKLREIMNLQSVSSDVGDPCFHPGRQCGHCGGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MV04</accession>
    <name>A0A3B5MV04_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MV04"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023624.1">
        <property type="protein sequence ID" value="ENSXCOP00000023344.1"/>
        <property type="gene ID" value="ENSXCOG00000017433.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166048"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="1">
        <location>
            <begin position="94"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="217" mass="24374" checksum="A1B9ECF9A35BA7E6" modified="2018-12-05" version="1">VDLNTGLVKEHISEMERRVGGQGTEPKMSIPVAFRQGTEPKMSIPIDFRQGTEPEMSHLIELRKEAQQNMQLPVAFRLATRVQTHKMACNGEIISGKCYEFNPNSLSYKDAFCRNLAPDAELASITNRDLHSRLVSLVTKGGENHPVLTWLGGIVKNQQASWVDGSKWSYSDWMPGHPNIHTDKPACVEMFKIDESWWSVADCELKRASLCSYPVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVD1</accession>
    <name>A0A3B5MVD1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="function">
        <text evidence="20">Pore-forming subunit of a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na+ ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="21">Voltage-gated sodium (Nav) channels consist of an ion-conducting alpha subunit which is functional on its own associated with regulatory beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 22">Cell membrane</location>
            <topology evidence="1 22">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.4/SCN4A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000027516"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027850.1">
        <property type="protein sequence ID" value="ENSXCOP00000027516.1"/>
        <property type="gene ID" value="ENSXCOG00000020541.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163423"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006110">
        <property type="term" value="P:regulation of glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904059">
        <property type="term" value="P:regulation of locomotor rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002082">
        <property type="term" value="P:regulation of oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036269">
        <property type="term" value="P:swimming behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099536">
        <property type="term" value="P:synaptic signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd13433">
        <property type="entry name" value="Na_channel_gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000002">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000001">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000003">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000006">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000002">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000004">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000005">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1190:FF:000001">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000003">
        <property type="entry name" value="Voltage-dependent sodium channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1190">
        <property type="entry name" value="iswi atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058542">
        <property type="entry name" value="IQ_SCN5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044564">
        <property type="entry name" value="Na_chnl_inactivation_gate"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010526">
        <property type="entry name" value="Na_trans_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF278">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN TYPE 2 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24609">
        <property type="entry name" value="IQ_SCN5A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06512">
        <property type="entry name" value="Na_trans_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="23" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 22" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 22" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="11 22" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 22" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="16 22" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 22" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9 22" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="347"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="476"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="506"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="541"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="586"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="676"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="847"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="886"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="917"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="963"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1086"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1168"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1198"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1228"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1285"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="1389"/>
            <end position="1412"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="25">
        <location>
            <begin position="125"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="25">
        <location>
            <begin position="475"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium ion transport-associated" evidence="26">
        <location>
            <begin position="771"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="25">
        <location>
            <begin position="845"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="25">
        <location>
            <begin position="1167"/>
            <end position="1422"/>
        </location>
    </feature>
    <feature type="domain" description="SCN5A-like C-terminal IQ motif" evidence="27">
        <location>
            <begin position="1534"/>
            <end position="1566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="28"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="771"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1590"/>
            <end position="1640"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="371"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="28"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="1600"/>
            <end position="1614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="1615"/>
            <end position="1640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00038083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00055248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00064899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU361132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF06512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF24609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1640" mass="187245" checksum="07ECAFAC9D11A8B3" modified="2018-12-05" version="1">MAQLLVPPGPDSFRPFVPKSLAAIERRIAEEEARRPRAERRSDDDDENRPKPNSDLEAGKSLPFIYGDIPPGLVSTPLEDLDPFYSNQKTFIVLNRGKAIFRFNATSALYILNPFNPLRRIAIKVLVMLIMFTILTNCAFMTLSNPPEWAKNVEYTFTGIYTFESLIKILARGFCVGKFTFLRDPWNWLDFSVILMAYVTEFVDLGNVSALRTFRVLRALKTISVIPGERAIKAHKVNIQPTGCLFCSVSLISCMMRLRLLSLCLPSSVGNYYHLPGRRDALLCGNGSGAGLCPEGFMCLKTGRNPDYGYTSFDTFSWAFLSLFRLMTQDFWENLYQQTLRAAGKPYMIFFVLVIFLGSFYLINLILAVVAMAYDEQNQATIEEAQQKEEEFQAMLEQLKRQQEEAQVKSSPPTTTFNFTSSVASILTNTMEELEESRQKCPPCWYRFANTCLIWDCCPAWLKIKEFVSMVVMDPFVDLAITICIVLNTLFMAMEHYPMTREFDNLLSIGNLVFTGIFTAEMCFKIIALDPYYYFQEGWNIFDGIIVSLSLMELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKISDNCELPRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCIIVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDSEMNNLQIAVGRIQRGISYVKTKVRLAPKTNEHQCSQTASCDCVLPPSAPSMQKLNIEPQPLSSSEGSTVDIRPPGDGAESVELEPEESLDPEACFTEGCVSRFQCCQVSEEVGWFKSWWTLRKTCFIIVEHNWFESFIIFMILLSSGALAFEDVYIEQRRTIKTVLEYADKVFTYIFILEMLLKWVAYGFVKYFTNAWCWLDFLIVDVSLVSLVANALGYSELSAIKTLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYHCINTTTDELFPISVVNNRTECLNLVNNSARWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRNLEEQPDYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKISCQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPTNAFQGFVFDCVTKQAFDIVIMILICLNMVTMMVETDDQTKEMDKILYWINLVFIVLFTGECTLKMISLRHFYFTVGWNIFDFVVVILSIVGMFLSEVIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFAMSNFAYVKRESGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNKREPDCDSKIEHPGNSYKGNCGNPSVGIFFFVSYIIICFLIVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWERFDPDATQFVEYNKLSDFADALDPPLRIAKPNMIQLIAMDLPMVSGERIHCLDILFAFTKRVLGEGGEMDMLRGQMEERFMASNPSKVSYEPITTTLRRKQEEMSAIVIQRAFRRYMIQQAMKKASALYKEQLKEGVRDPDKDVMVISKFNENSTSDKTDMTPSTASPPSYNSVTKSDKDKYEKENREKENKGKDLKDRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MVH8</accession>
    <name>A0A3B5MVH8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Non-selective voltage-gated ion channel VDAC3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane. Forms a high-conducting channel with a stable open state and a voltage-induced closure with a mild preference for anions over cations. Involved in male fertility and sperm mitochondrial sheath formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="18">Interacts with ARMC12 in a TBC1D21-dependent manner. Interacts with MISFA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic mitochondrial porin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MVH8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023549"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023830.1">
        <property type="protein sequence ID" value="ENSXCOP00000023549.1"/>
        <property type="gene ID" value="ENSXCOG00000017590.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182869"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001925">
        <property type="entry name" value="Porin_Euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743">
        <property type="entry name" value="VOLTAGE-DEPENDENT ANION-SELECTIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743:SF28">
        <property type="entry name" value="VOLTAGE-DEPENDENT ANION-SELECTIVE CHANNEL PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00185">
        <property type="entry name" value="EUKARYTPORIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-0626">Porin</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00046980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00050036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="100" mass="11072" checksum="C70242DD6F8FDF98" modified="2018-12-05" version="1">MDSPPTYTDLGKAAKDIFSKGYGFGAVKIELKTRSDNGVEFHSTGFSNMDTGKATGNLETKYKITDHSLTISQKWNTDNVLVNEVTVEDKVCLCFLLLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWN0</accession>
    <name>A0A3B5MWN0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">TIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Toll-like receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MWN0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025832"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026142.1">
        <property type="protein sequence ID" value="ENSXCOP00000025832.1"/>
        <property type="gene ID" value="ENSXCOG00000019304.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157975"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002224">
        <property type="term" value="P:toll-like receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:001164">
        <property type="entry name" value="GH01279p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000001">
        <property type="entry name" value="Toll-like receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000483">
        <property type="entry name" value="Cys-rich_flank_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365:SF522">
        <property type="entry name" value="LOW QUALITY PROTEIN: TOLL-LIKE RECEPTOR 13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24365">
        <property type="entry name" value="TOLL-LIKE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00082">
        <property type="entry name" value="LRRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="13" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017384022" description="TIR domain-containing protein" evidence="14">
        <location>
            <begin position="28"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="15">
        <location>
            <begin position="787"/>
            <end position="928"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="947" mass="109132" checksum="1083C940D0C841BC" modified="2018-12-05" version="1">MQCQWCVLSHFALKLLCLLLHINFSLPYSLKNCTIPHEESSSAEVSLDCANRKLVTIPDDILKDAVSVKLGHNFIQRVHQRDFCGMSKMKILDLEYNQVVQVDDGSFTDLVSLKALYMSKNQLTNLTSNVFQGLSSLTVLDLSQNQIQFIHESAFRVLTSLETVNFQDNMLQRIVDIQPILQLPQIHTIDLTYNLYYSFETKDLHLNFSSGLKELSVYSHNLIKFGITTPVFPHLHKLELSMSLENTSRWDLPDKPLLKNVTHLCLGILPLSNEEIQKALQTFDSIYHLRLNFIYIRIRDGLLAIACKIPTLRKLDLSIIDFSNYTLELAQCSQLTDLDLSICLISELPQGSMDSMRQMRFFNVSYNKLKKIPHDIRSLSSLEILIMNDNIISNLGCEDFVNTTHLTELHLNGNRIAKLQRCVLESLTNLKLLNLSDNLLVTFKASTKVGLQKLEILDLSQNFIKLLNNYDFRGLRSLKHLNVTSYFLENLKYYTFLGLKSLKSFSGSFPLYLVNLRTLPQLEKIQVNFNYNGPFRTPYLKTYEDSIRIKSLKSVSMAHQGLLYYLAEGVLHNLKHLEYFKAENIYLSAPYSDTFQFNQKLRSLIFTKTDLSELQPELFLPIPNLESLDLSESQLKSLDFLVHANLSALRYLKLTGNELNVINKTVFQSLPSLTFLDLDKNPFTCECSSADFIQWAKSNTQTQVVNAHQYVCSFPVSRRGSLLLDFNFQPCLDDGGFLCFVSSACLVALTLLSSFVYHFLRWHLTYTFHLLLSFLYESRKGRKRDLHQFDAFVSYNVHDEDWVYREMLPVLEGQQGWRLCLHHRDFQPGKPIIENITDAIYGSRKTICVISRSYLQSEWCSREIQMASFRLFDEKKDVLILLFLEEIPARHLSPYYRMRKLVKKRTYLSWPQAAQHPGVFWQNVQRALQTGGAVAENTDLLAGPAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MX56</accession>
    <name>A0A3B5MX56_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MX56"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024764.1">
        <property type="protein sequence ID" value="ENSXCOP00000024470.1"/>
        <property type="gene ID" value="ENSXCOG00000018254.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234640"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF847">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="193"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="310" mass="35499" checksum="2B417CBDB08F1912" modified="2018-12-05" version="1">MDGEVNETYLTLDGHVELYKYRYLYFVVTLTVYIFIVISNLTIVYVIWTHENLHEPMYVFIAALLLNSVVFSTNIYPKLLSDFLSEKQIISYSACYVQFFLYYCLGASEFSLLAAMAYDRYVSICKPLQYATIMGRNTVSIYLSLAWLVPACLIAVMVILNVNVKLCKYNINGILCNNSIYGLHCVDSKVQTVFGAVVLVLTLLFPLLFIVFTYSRILIMSYRSSKEVRKKAAQTCSPHLLVLISFSCLCAFDVIIARLGSNLTNLIRLIMTLQAVLYHPLFNPIIYGLKMKEISKQLRRLFSKAKVMHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MX78</accession>
    <name>A0A3B5MX78_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nurim</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Nuclear envelope membrane protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Nuclear rim protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nurim family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027892192.1">
        <property type="nucleotide sequence ID" value="XM_028036391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MX78"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026510.1">
        <property type="protein sequence ID" value="ENSXCOP00000026192.1"/>
        <property type="gene ID" value="ENSXCOG00000019568.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114156133"/>
    <dbReference type="KEGG" id="xco:114156133"/>
    <dbReference type="CTD" id="11270"/>
    <dbReference type="GeneTree" id="ENSGT00390000008146"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033580">
        <property type="entry name" value="Nurim-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31040">
        <property type="entry name" value="NURIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31040:SF1">
        <property type="entry name" value="NURIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="276" mass="30683" checksum="8D17BDE5BB486508" modified="2018-12-05" version="1">MASVTARSWALCAVSLLNLLFVFISGADFIRFVSFRPIYHNITGETTLCQDSVPWPVALKDSSVLWALVVDLSLLALFVAQHSLMAWAPVKRALQTVLGALNRTAYCFTTALALQILMHHWQPVTGAPCLWSVRHAPWSVWFPLLCFALHFLCWAIICSILVIFDYPELLGIKQVYYECLGLGDPWAQKPAPAQRLLSHLRHPVCLELAVILWLLPAFSLDRLLLAGTLSAYVGLAHSLDKQDLAYLCAQLKGKVWLLAELQCGGVEQNGSEEKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXE8</accession>
    <name>A0A3B5MXE8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Semaphorin 6D</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXE8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024650"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024948.1">
        <property type="protein sequence ID" value="ENSXCOP00000024650.1"/>
        <property type="gene ID" value="ENSXCOG00000018419.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159303"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000025">
        <property type="entry name" value="Sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D, like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003671">
        <property type="entry name" value="Semaphorin 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11036:SF65">
        <property type="entry name" value="SEMAPHORIN-6D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01403">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101912">
        <property type="entry name" value="Sema domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017425077" evidence="8">
        <location>
            <begin position="25"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="576"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="Sema" evidence="9">
        <location>
            <begin position="1"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="332"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="708"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="844"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="708"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="731"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="767"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="877"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="910"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="992"/>
            <end position="1004"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1015" mass="112268" checksum="63EF14468FC8F3A7" modified="2018-12-05" version="1">MGQGAALLLSKLLLLLAASRTLLAVSFPEDIVPLDVVDAHFTRRYPVFRGRPSVNDSQHRLDFQLMTKIQDTLFIAGRDQVYLVSLRESYRNEIISYRDECHNFIKVLVPRNDDLVFICGTNGFNPMCRYYRLDNLEFDGEEINGLARCPFDSKQTNVALFAEGKLYSATVADFQASDSVIYRSMGDGSALRTIKYDSKWLKEPHFLHAAEFGNYVYFFYREIAVEHSNLGKVVYSRVARICKNDVGGSQRVLEKHWTSFVKARLNCSVPGESFFYFDVLQSITDIIDVNGVPSVVGVFTTQMNSIPGSAVCAFSMTDIEKVFMGRFKEQKTPDSVWTPFPEEKLPKPRPGSCAGHGPAASFKSSVEFPDDTLQFIKSHPLMDTAVPSIGDEPWFTKTRVRYRLTALAVDGQAGPHKNYTVVFIGAESGVVLKVLAKTSLLSLNESLLLEEIDVFNRAKCPSNREDDKRVLSLHVDNNTHSLYVAFSSCVIRIPLSRCERHSSCQKSCIASRDPYCGWKPHGACERIQPGVFLSLHIFRKGYEQDVEYGNTTHLGDCQGVWDIRTGDSNQMVHMNILITCVFAAFLMGALLAGLIVFCYRDSVLRKPRHVHKDMESAPSCSDSTGSFVKLNGLFDSPVKEYQSNIDSPKLFTNLLSNGKDLNTPNGDTKTMILRDGCQPPELAALPTPESTPVLQQKGLQPIKNQWEKAHGKVSGPHKEANPSAKSPQFLSSSPAPANASSNHPHIALGHSNIPSAVVLPNATHDQPNLDHGDETLPHSSEKQLKIPDCKGNRKDQKRSVDARNTLNDLLKHLNDSVANPKAILQEESGPRPRPHLMLEPMEELTEVPPAVPSREASLYSPSSSLPRHSPTKRVDVPMPSTPTTPTGSLSMGGTLERQRGGYQLHRSASHRQSLSTSPNGVTMGVSVSRQHSMNRGGYMPPTPPSRLDSHGAMVASGMHSPHPPSVSRQSSYSGHGSLPRTGLKRTPSLKPDVPPKPNGFPPQTPQMRVVNKYSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZ99</accession>
    <name>A0A3B5MZ99_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cytochrome P450, family 20, subfamily A, polypeptide 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MZ99"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027457"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027790.1">
        <property type="protein sequence ID" value="ENSXCOP00000027457.1"/>
        <property type="gene ID" value="ENSXCOG00000020500.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00500000044939"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052666">
        <property type="entry name" value="CYP450_20A1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24280">
        <property type="entry name" value="CYTOCHROME P450 20A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24280:SF4">
        <property type="entry name" value="CYTOCHROME P450 20A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="217" mass="24008" checksum="F5E86B7EE4265548" modified="2018-12-05" version="1">MLDFAIFAVTFVIILVGAVLYLYPSSRRASGVPGLYPTDEKDGNLQDIINQGSLHEFLVNLHQEFGSVASFWFGGRPVVSLGSVNQLKQHINPNHTTDSFETMLKSLLGYPPGMGGGPNESVVRKKLYEGAINNSLKNNFPVVLKVAEELVGKWKSFPEAQHTPLCAHLLGLALKTVTQLGLGERFKDDAEIISFRKNHDAVRIISIFSFFSFNQNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZJ4</accession>
    <name>A0A3B5MZJ4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methanethiol oxidase</fullName>
            <shortName evidence="3">MTO</shortName>
            <ecNumber evidence="3">1.8.3.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Selenium-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the oxidation of methanethiol, an organosulfur compound known to be produced in substantial amounts by gut bacteria. Selenium-binding protein which may be involved in the sensing of reactive xenobiotics in the cytoplasm. May be involved in intra-Golgi protein transport.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>methanethiol + O2 + H2O = hydrogen sulfide + formaldehyde + H2O2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16007"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="EC" id="1.8.3.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Organosulfur degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <text evidence="3">May associate with Golgi membrane. May associate with the membrane of autophagosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the selenium-binding protein family.</text>
    </comment>
    <dbReference type="EC" id="1.8.3.4" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MZJ4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027962.1">
        <property type="protein sequence ID" value="ENSXCOP00000027627.1"/>
        <property type="gene ID" value="ENSXCOG00000020615.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000244530"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018549">
        <property type="term" value="F:methanethiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008430">
        <property type="term" value="F:selenium binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008826">
        <property type="entry name" value="Se-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23300">
        <property type="entry name" value="METHANETHIOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23300:SF0">
        <property type="entry name" value="METHANETHIOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05694">
        <property type="entry name" value="SBP56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0711">Selenium</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="71"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU369071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="132" mass="14530" checksum="A3CACF4FFC89DC54" modified="2018-12-05" version="1">MYSLNLFCLVNKLVVTIATRKTTVLVPVQSEVLVLPAGPRERIVYLPCIYRSTAVQKPDYLATVDIDPESPTYCQVEPNQNHDPVLLDSSNSSGSTSEPQPRSTPGSVGSVWFYLVLFGSVGSVGSVWFCWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N0I8</accession>
    <name>A0A3B5N0I8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Musculoskeletal embryonic nuclear protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MUSTN1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N0I8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026660"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026986.1">
        <property type="protein sequence ID" value="ENSXCOP00000026660.1"/>
        <property type="gene ID" value="ENSXCOG00000019914.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175564"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002062">
        <property type="term" value="P:chondrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035988">
        <property type="term" value="P:chondrocyte proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042246">
        <property type="term" value="P:tissue regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031394">
        <property type="entry name" value="MUSTN1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15682">
        <property type="entry name" value="Mustang"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="79" mass="8591" checksum="841F08E898F4E6F5" modified="2018-12-05" version="1">MSLEEDGSQITFSLERKRKEEDLTEAKTKLGGGGAVKSKTFEVMEECEKMGKAAPSVFSGARSGGETAFNTRSTRQARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N175</accession>
    <name>A0A3B5N175_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cyclic nucleotide-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N175"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027270"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027603.1">
        <property type="protein sequence ID" value="ENSXCOP00000027270.1"/>
        <property type="gene ID" value="ENSXCOG00000020365.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158737"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017071">
        <property type="term" value="C:intracellular cyclic nucleotide activated cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030553">
        <property type="term" value="F:cGMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005222">
        <property type="term" value="F:intracellularly cAMP-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005223">
        <property type="term" value="F:intracellularly cGMP-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000002">
        <property type="entry name" value="Cyclic nucleotide gated channel alpha 1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.630:FF:000001">
        <property type="entry name" value="Cyclic nucleotide-gated channel alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000030">
        <property type="entry name" value="Cyclic nucleotide-gated channel alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.630">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032406">
        <property type="entry name" value="CLZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050866">
        <property type="entry name" value="CNG_cation_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638:SF6">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL ALPHA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16526">
        <property type="entry name" value="CLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="244"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="12">
        <location>
            <begin position="348"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="502"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="596" mass="68056" checksum="B59E54ED3D73E070" modified="2018-12-05" version="1">MNSTLSSLMTLLSTHRGLSTRKEVWIMDPASDMYYNWLCTVSVPVFYNLMFLVARACFNELQYANSTLWMALDYISDVIYYVDTFVRVRTGFLEQGLLVKDEKVLKEKYTKTLQFKLDMMSIIPTDFVFLYIGINNPEWRFNRLFRSARLFEFFDRTETRTNFPNIFRIANLVLYIIIIIHWNACLYFAVSKTLGFGTDTWVYPNMSNPDYARLTRQYIYCFYWSTLTLTTIGETPPPVRDIEYFFVVADFLTGVLIFATIVGNVGAMISNMNAARVEFQAKIDSIKQYMQFRKVTKDLEARVVKWFDYLWTEGKSCDEKLVLKNLPDKLKAEIAINVHLETLRKVRIFQDCEAGLLVELVLKLQPQVFSPGDYICKKGDIGREMYIIKEGKLAVVADDGVTQFVVLSDGAYFGEISILGIKGSKAGNRRTANIRSVGYSDLFALSKDDLMEALIEYPDAKYALEDKGRAILMKDNLIDESLVAAVDAKDLEDKVNRIEGSVDVMTIKLRKLTNQYESSQRKLNQRLANLSNEVKSLRDDEAGIHGLSEKESVSSGGSEHAKPFGIGDGFQQSLSQLVLVAVFWQQQHVKAGVGCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N1U4</accession>
    <name>A0A3B5N1U4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Anamorsin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cytokine-induced apoptosis inhibitor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Fe-S cluster assembly protein DRE2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CIAPIN1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis, facilitating the de novo assembly of a [4Fe-4S] cluster on the scaffold complex NUBP1-NUBP2. Electrons are transferred to CIAPIN1 from NADPH via the FAD- and FMN-containing protein NDOR1. NDOR1-CIAPIN1 are also required for the assembly of the diferric tyrosyl radical cofactor of ribonucleotide reductase (RNR), probably by providing electrons for reduction during radical cofactor maturation in the catalytic small subunit. Has anti-apoptotic effects in the cell. Involved in negative control of cell death upon cytokine withdrawal. Promotes development of hematopoietic cells.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer. Interacts with NDOR1. Interacts with CHCHD4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The C-terminal domain binds 2 Fe-S clusters but is otherwise mostly in an intrinsically disordered conformation.</text>
    </comment>
    <comment type="domain">
        <text evidence="11">The N-terminal domain has structural similarity with S-adenosyl-L-methionine-dependent methyltransferases, but does not bind S-adenosyl-L-methionine. It is required for correct assembly of the 2 Fe-S clusters.</text>
    </comment>
    <comment type="domain">
        <text evidence="11">The twin Cx2C motifs are involved in the recognition by the mitochondrial CHCHD4/MIA40-GFER/ERV1 disulfide relay system. The formation of 2 disulfide bonds in the Cx2C motifs through dithiol/disulfide exchange reactions effectively traps the protein in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the anamorsin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N1U4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027800"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028135.1">
        <property type="protein sequence ID" value="ENSXCOP00000027800.1"/>
        <property type="gene ID" value="ENSXCOG00000020726.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011417"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03115">
        <property type="entry name" value="Anamorsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007785">
        <property type="entry name" value="Anamorsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049011">
        <property type="entry name" value="Anamorsin_N_metazoan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046408">
        <property type="entry name" value="CIAPIN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13273">
        <property type="entry name" value="ANAMORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13273:SF14">
        <property type="entry name" value="ANAMORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20922">
        <property type="entry name" value="Anamorsin_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05093">
        <property type="entry name" value="CIAPIN1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 11" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anamorsin N-terminal" evidence="13">
        <location>
            <begin position="9"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Anamorsin N-terminal" evidence="13">
        <location>
            <begin position="81"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Anamorsin C-terminal" evidence="12">
        <location>
            <begin position="213"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Anamorsin C-terminal" evidence="12">
        <location>
            <begin position="251"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Fe-S binding site B" evidence="11">
        <location>
            <begin position="254"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cx2C motif 1" evidence="11">
        <location>
            <begin position="254"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cx2C motif 2" evidence="11">
        <location>
            <begin position="265"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="292" mass="31548" checksum="CD85B61111BC13BB" modified="2018-12-05" version="1">MADLGIKAGDKVLLVWTQPSTPETLKQYAEELGAAVGADGKISVENMERLSLCESIAHQFNYSTQLSLIVETSIKVYPVLSTEAQTMRTAEKLMSALKLSGFMSVTEVSKTELSPETLSSLRAATGYQGNTLTRVRISASKPNYEVGSSSQIKLSFGKKTQKPAEKLALDPNTKKMWMLSANDMNDDDVDLLDSDTLLDEDDLKKPDPASLKASSCGEGTGKKKACKNCTCGLAEELEQESKENQKTNLPKSACGSCYLGDAFRCASCPYLGMPAFKPGEKIVLDNNTLTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N1Z6</accession>
    <name>A0A3B5N1Z6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Troponin I4b, tandem duplicate 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N1Z6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027930"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028266.1">
        <property type="protein sequence ID" value="ENSXCOP00000027930.1"/>
        <property type="gene ID" value="ENSXCOG00000020829.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234588"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060048">
        <property type="term" value="P:cardiac muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003009">
        <property type="term" value="P:skeletal muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.350:FF:000008">
        <property type="entry name" value="Troponin I4b, tandem duplicate 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050875">
        <property type="entry name" value="Troponin_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738:SF12">
        <property type="entry name" value="TROPONIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738">
        <property type="entry name" value="TROPONIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="22113" checksum="0BDB47F04003B0F7" modified="2018-12-05" version="1">TGEPSSEPLRTSHLIHKPKPKISASRRLFLKTKLLKKAMIMLENEKQARIADKQRILDERHPTLQLSGLSMQDLQNLCKEIHQKIDKVDEERYDIASKVAINEKEIQNLSQKVFELKGKMKRPNLRKVRVSAGAMLGALLGAKVKESVDFKANLKTVKKEEEKVRKEEVTDWRKNVDAMSGMEGRKKLFDAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KIY7</accession>
    <name>A0A3B5KIY7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Glutathione synthetase</fullName>
            <shortName evidence="15">GSH-S</shortName>
            <ecNumber evidence="4 15">6.3.2.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the production of glutathione from gamma-glutamylcysteine and glycine in an ATP-dependent manner. Glutathione (gamma-glutamylcysteinylglycine, GSH) is the most abundant intracellular thiol in living aerobic cells and is required for numerous processes including the protection of cells against oxidative damage, amino acid transport, the detoxification of foreign compounds, the maintenance of protein sulfhydryl groups in a reduced state and acts as a cofactor for a number of enzymes. Participates in ophthalmate biosynthesis in hepatocytes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>gamma-L-glutamyl-(2S)-2-aminobutanoate + glycine + ATP = ophthalmate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:72075"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:189406"/>
            <dbReference type="ChEBI" id="CHEBI:189750"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:72076"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13557"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:13558"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15 17">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 15">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the eukaryotic GSH synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.3" evidence="4 15"/>
    <dbReference type="RefSeq" id="XP_027877900.1">
        <property type="nucleotide sequence ID" value="XM_028022099.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027877981.1">
        <property type="nucleotide sequence ID" value="XM_028022180.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027878063.1">
        <property type="nucleotide sequence ID" value="XM_028022262.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027878140.1">
        <property type="nucleotide sequence ID" value="XM_028022339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KIY7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000058"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000058.1">
        <property type="protein sequence ID" value="ENSXCOP00000000058.1"/>
        <property type="gene ID" value="ENSXCOG00000000045.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114147582"/>
    <dbReference type="KEGG" id="xco:114147582"/>
    <dbReference type="CTD" id="2937"/>
    <dbReference type="GeneTree" id="ENSGT00390000013764"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004363">
        <property type="term" value="F:glutathione synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.50:FF:000001">
        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1760:FF:000001">
        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1760">
        <property type="entry name" value="Glutathione synthase, substrate-binding domain superfamily, eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1080.10">
        <property type="entry name" value="Glutathione Synthetase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005615">
        <property type="entry name" value="Glutathione_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014042">
        <property type="entry name" value="Glutathione_synthase_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014709">
        <property type="entry name" value="Glutathione_synthase_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014049">
        <property type="entry name" value="Glutathione_synthase_N_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037013">
        <property type="entry name" value="GSH-S_sub-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004887">
        <property type="entry name" value="GSH_synth_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01986">
        <property type="entry name" value="glut_syn_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11130">
        <property type="entry name" value="GLUTATHIONE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11130:SF0">
        <property type="entry name" value="GLUTATHIONE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03917">
        <property type="entry name" value="GSH_synth_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03199">
        <property type="entry name" value="GSH_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001558">
        <property type="entry name" value="GSHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 15" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="6 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="11 15 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 15 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutathione synthase substrate-binding" evidence="19">
        <location>
            <begin position="205"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="149"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="215"/>
            <end position="217"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="268"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="366"/>
            <end position="375"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="400"/>
            <end position="403"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="452"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="460"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="463"/>
            <end position="464"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00059746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR001558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001558-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR001558-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR001558-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF03199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="476" mass="53283" checksum="588467995F6B5888" modified="2018-12-05" version="1">MASSIPDEVMMDAALIKELVEVAKDAALLQGVLMRTKESPNSSELVTYAPFTLFPSPVPKSVFLQALAVQTHFNTLVDKISQDMDFLQEALASTIEVDDFTARLFKIHQQILQEGRSQSIVLGLNRSDYMLDQREDQNSSLKQIEINTISASFGGLSSQTPHVHRHILKVAGRLEESQRILDNNPAAGLARGLAKAWELYGSERAVVMFLVEVDQRNIFDQRYIESELWKRNIPTVRKRFDDVYKTGSLDPDKRLFVDGKEVAVVYFRNGYMPQNYTNEQCWDVRLMMERSQAVKCPDISTHLAGTKKIQQVLARPGVLERFFPDQPQVVQQIKETFTGLYTLDMGPEGDKTVAMALAEPNRFVLKPQREGGGNNIYGSEIVQVLKEVRGSPERTAYILMDKINPTPIQNYLLRRDAPLAISNCLSELGVFGAYIRQGAHMVMNECVGHLLRTKSSEHADGGVAAGVAVLDNPLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KKG1</accession>
    <name>A0A3B5KKG1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">P53 apoptosis effector related to PMP22</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMEM47 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027862378.1">
        <property type="nucleotide sequence ID" value="XM_028006577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KKG1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000663"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000673.1">
        <property type="protein sequence ID" value="ENSXCOP00000000663.1"/>
        <property type="gene ID" value="ENSXCOG00000000578.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114137768"/>
    <dbReference type="KEGG" id="xco:114137768"/>
    <dbReference type="CTD" id="64065"/>
    <dbReference type="GeneTree" id="ENSGT00530000063484"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015664">
        <property type="entry name" value="P53_induced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14399:SF4">
        <property type="entry name" value="P53 APOPTOSIS EFFECTOR RELATED TO PMP-22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14399">
        <property type="entry name" value="P53-INDUCED PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="20642" checksum="ACB49C19A1D349C6" modified="2018-12-05" version="1">MFRCGLAYPRCRWIMPLLLIFAIIFDIIAIAAQSGWIQDRDNTHYSSMWKDCRRMGDESDCMSLMDESWARAVAALMIIGLLILIIAFVVSLVALCNDVSVGACITVGVLLFIVALLQFIGLIIYPVNFNDRIFEGEYEYTWAYGFGWGATIICLGCSFIFCCITSCISKINGDEKMEYCITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KNW6</accession>
    <name>A0A3B5KNW6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Phosphoinositide-binding protein which associates with both cell and endoplasmic reticulum (ER) membranes. Can bind to the ER membrane protein VAPA and recruit VAPA to plasma membrane sites, thus linking these intracellular compartments. The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface. With VAPA, may regulate ER morphology. Has a role in regulation of the actin cytoskeleton, cell polarity and cell adhesion. Binds to phosphoinositides with preference for PI(3,4)P2 and PI(3,4,5)P3. Also binds 25-hydroxycholesterol and cholesterol.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer. Interacts with RRAS. Interacts (phosphorylated form) with VAPA. Interacts with OSBPL6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Filopodium tip</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 19">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KNW6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001828"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001855.1">
        <property type="protein sequence ID" value="ENSXCOP00000001828.1"/>
        <property type="gene ID" value="ENSXCOG00000001478.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155957"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032433">
        <property type="term" value="C:filopodium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006699">
        <property type="term" value="P:bile acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13287">
        <property type="entry name" value="PH_ORP3_ORP6_ORP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000011">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000001">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3490:FF:000004">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041680">
        <property type="entry name" value="PH_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF150">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15409">
        <property type="entry name" value="PH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12 20" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="13" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="22">
        <location>
            <begin position="50"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00064163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU003845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="799" mass="90952" checksum="15524471CA0FB8A9" modified="2018-12-05" version="1">HRSMTSSCEKMTSLSPTHSDSSGSSKHDTNQDSWEIVEGLRGAPASIQEPDRQEGFLLKRRKWPMKGWHKRYFVLERGILKYAKGPADLQKGKVHGLIDVGLSVMSIKNKAMCVDLDTEDNIYHLKVKAPELFDEWVSKLRHHRMFRQNEIALYPHETYLFHPHAASSPSLNDSLRKRATLTKQASFHQSKVSSWLHSSEEMSKCSKELEECESYLLELNQLLKSMEVIHRTYSAPAITALQVSAAARNYVRFEPGQRNAVSTCVCFLLYGSIVSMTTPVPRFFPVSGFCLSPVHVSLKSAYGSLVAERERLKHTIDLQAPQPPEVVGLKNLYASEGTSSPHCLVHQASGESRVSIPESISEFFDAQEYLLSSSSSENEVSDDDSYISDVSDTNLDLKMSSADSDLCFKRPVPPAVSSVMTLTRRRSTLPSTCPSSTVSLWNILRNNIGKDLSKVAMPVQLNEPINTLQRLCEELEYSELLDIANQTEDPYQRMVYVATFAISAYASSYYRAGSKPFNPVLGETYECDRPDKGFRFIAEQVSHHPPVSACHSDSKHFCFWQDVRWKNKFWGKSMEIVPMGTTHVTLPAFGDHYEWNKVTSCIHNILSGQRWIEHYGEMAIKNKNSNVCQCKVTFVKAKSWNSTVNEIEGVVTDSNGKVVHTIFGKWHESVFQGDPPSATCIWRANPMPVEQEQYYGFTQFAVELNELDSSLKPLLPPTDTRFRPDQRLLEEGNIDGAEEQKQRIEQLQRERRRVLQDNSMIHQPRFFKKSKDDTWVSNNTYWEQRRDPGFSKVDFPVLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KT47</accession>
    <name>A0A3B5KT47_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pentatricopeptide repeat-containing protein 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PTCD2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KT47"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001153.1">
        <property type="protein sequence ID" value="ENSXCOP00000001140.1"/>
        <property type="gene ID" value="ENSXCOG00000000912.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009329"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034629">
        <property type="entry name" value="PTCD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14700">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14700:SF0">
        <property type="entry name" value="PENTATRICOPEPTIDE REPEAT-CONTAINING PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017291179" description="Pentatricopeptide repeat-containing protein 2, mitochondrial" evidence="4">
        <location>
            <begin position="19"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="3">
        <location>
            <begin position="128"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="351" mass="39856" checksum="3235EADD1A2931C4" modified="2018-12-05" version="1">MFAFSLFYLFSFLSTAKRHLLSEDVIKLQDFQQKKLAVAHLVTGSDGEKLNELILRDDLKLLLHLCQTPDDMLVAQKAIYRYHAENRNLLYGDFKFGPLFMRLCYELGMEGMAAATLTDKNMKGFFIDSTSFNIAINMLFVKGSYEGAMDVLRTMRSQGVPFNKDTLTLAMATCYKLNTAECYRISMSLIEEEQTRGQFIPRHAYCFAVALALRRNDLEKAHSMYSQIMSTDSKLCQNFNVMILTMSGDVLGAVSVLSAAISPSRSFFVKKPEFSKEVIDLLVLRSEGTPFKMKAEQVVTQLQQAGQVTQETLDDMLCRVPTGKRKPVVMEVVRRTSRRTRRSLQSNLLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTG7</accession>
    <name>A0A3B5KTG7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTG7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001265"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001283.1">
        <property type="protein sequence ID" value="ENSXCOP00000001265.1"/>
        <property type="gene ID" value="ENSXCOG00000001042.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153500"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="146" mass="16201" checksum="F2A245B047BD74A1" modified="2018-12-05" version="1">MFLPVTLDVWDHSGLSDYDGLRRLSFPQTDVLLLCFSLTSPSSFENVRSKWTDEVRHHCPAVPVVLVGTMLDLRDDKDRIEKLKKEHLSPITYDQGLAMAEEIGAATYLECSALTELGLQAVIEEAARVGLNAKPVNKPKQSCSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTJ1</accession>
    <name>A0A3B5KTJ1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the troponin I family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTJ1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000931"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000943.1">
        <property type="protein sequence ID" value="ENSXCOP00000000931.1"/>
        <property type="gene ID" value="ENSXCOG00000000275.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234588"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005861">
        <property type="term" value="C:troponin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060048">
        <property type="term" value="P:cardiac muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003009">
        <property type="term" value="P:skeletal muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001978">
        <property type="entry name" value="Troponin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050875">
        <property type="entry name" value="Troponin_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038077">
        <property type="entry name" value="Troponin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738">
        <property type="entry name" value="TROPONIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13738:SF31">
        <property type="entry name" value="TROPONIN I TYPE 2B (SKELETAL, FAST), TANDEM DUPLICATE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00992">
        <property type="entry name" value="Troponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90250">
        <property type="entry name" value="Troponin coil-coiled subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="178" mass="20666" checksum="3E1DC637EAF06D64" modified="2018-12-05" version="1">GCLDNFSCMFPFSCQSLLLQIGKEMLEEEARQAEEEKMAYIRENCPTLSFPQCTLELQELCRQLHKQIDLVDEERYDMETKVKKCNKEIDDLKMKVQDLNGRFKKPTLRRVRMSADAMLAALLGSKHKVSLDLRANLKQVKKELKEEVSAEKQTGDWRKNIEDKAGMDGRKKMFEAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTY6</accession>
    <name>A0A3B5KTY6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cilia- and flagella-associated protein 251</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CFAP251</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTY6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001435"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001458.1">
        <property type="protein sequence ID" value="ENSXCOP00000001435.1"/>
        <property type="gene ID" value="ENSXCOG00000001148.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013370"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050630">
        <property type="entry name" value="WD_repeat_EMAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720:SF13">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 251"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720">
        <property type="entry name" value="WD-40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="892"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="8"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="921" mass="104067" checksum="86A6D99D03D2665A" modified="2018-12-05" version="1">VWSSGIMSASSESSEEPTLTSAEEPQISEHAGEEENVQTTCDISNMHGQSQVYTATQDTLFSKEAARPAMHALSLERVFGMNPVLPIYSLQDSNQLIFVYASAHVGVIFNHTLNSQHILQGHSCPISCMCVSEDRRWIATVDTGPRNMVIIWDSYSGIPVNTLFECHPGSCVAALGFSSDTKYLATLGNAEFQSVLIWDWTSTEQKCLCQAELNPKHGFQDHIIFNPNDSSQLLSGSKTHMLIYKWDSEGLEYFALIPFADLCLSRSVFHWKKNLVLKAIGSSIVVWDIGKNLIEKQTKSLGGIKTIHIQQEPITVLTITDTFIVTGDIQGNVRFYDENFLLVNWYTDFNQDPVVYISFSKELPKDNDEEDYFLDVDPVIIRNFIISTINAKIIHLNTQTRVIQTVLQNDYDPLHALTCHPFQPAVAFGNLQGMLRLWDYNCKAIIGNRIFETEKQIQSITFDPKGLFLAVGFRTGAVYILNATTLENKPEEIFNCIKDSIHLITFSHDSQYLATADAGRTVSLFRNEGYKDSSSRWRFVGRYRSHYKPIQDLLFGVQLYNQQVEYDLEKSESNKLLLLSIKRIEQSAVPRCMTWYPFLNPEEFLLVASDNYKMKLFNSTTTMCRKTLLGPTYGSPIGKIAVLPKSTTSKTKSHHLAFITDDKLGLQILPLDGNPYKSSALICHPTGVSTFSCSYDGKLVFTAGGSDSSVLSWRVNLDVLDAAAALSGEDMDPFYSLIKGGRDGRFYKEMEDFFFYCQIQHQGTDMMKRIEVSTKIPLSEVPILMRALGHFPTEQELEEMFNEIKFSRFAETGKYVTDIDLEDFIKLYINHRPAFGISSEELVQAFHVLGKDNLVGNPIIHKSEMIKFLQARGEHMTEDEVAECFTTLLGVNEKEEQEEEALGEVRTSPQERRHQQPASKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KUU9</accession>
    <name>A0A3B5KUU9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Vascular endothelial growth factor Ba</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the PDGF/VEGF growth factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KUU9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001780"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001807.1">
        <property type="protein sequence ID" value="ENSXCOP00000001780.1"/>
        <property type="gene ID" value="ENSXCOG00000001442.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166453"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042056">
        <property type="term" value="F:chemoattractant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005172">
        <property type="term" value="F:vascular endothelial growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050930">
        <property type="term" value="P:induction of positive chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045766">
        <property type="term" value="P:positive regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001938">
        <property type="term" value="P:positive regulation of endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060754">
        <property type="term" value="P:positive regulation of mast cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002040">
        <property type="term" value="P:sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048010">
        <property type="term" value="P:vascular endothelial growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038084">
        <property type="term" value="P:vascular endothelial growth factor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00135">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023581">
        <property type="entry name" value="PD_growth_factor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000072">
        <property type="entry name" value="PDGF/VEGF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050507">
        <property type="entry name" value="PDGF/VEGF_growth_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12025:SF14">
        <property type="entry name" value="SNAKE VENOM VASCULAR ENDOTHELIAL GROWTH FACTOR TOXIN VR-1'-LIKE ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12025">
        <property type="entry name" value="VASCULAR ENDOTHELIAL GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00341">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00141">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00249">
        <property type="entry name" value="PDGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50278">
        <property type="entry name" value="PDGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 3" id="KW-0339">Growth factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Platelet-derived growth factor (PDGF) family profile" evidence="4">
        <location>
            <begin position="11"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="170" mass="19281" checksum="F7DFB2EEEB7911C4" modified="2018-12-05" version="1">ADSPRSREGREVRRWLEVYSRSGCEPRDTLVEVWRELPGEIHHLFVPSCVSVKRCGGCCADEALECVPSLTHTLTMELMRTSFMKHELIELPFVEHSQCEFMEYLLFTDPTALLSMSAHRLKEQFQPTPTTRWKSSPGSLSAASCKVALIPNVCCKVDLVQGCNRYINGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KX53</accession>
    <name>A0A3B5KX53_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000002605"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002640.1">
        <property type="protein sequence ID" value="ENSXCOP00000002605.1"/>
        <property type="gene ID" value="ENSXCOG00000002086.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155263"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032483">
        <property type="term" value="P:regulation of Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01235">
        <property type="entry name" value="PH_Sbf1_hMTMR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11500:FF:000006">
        <property type="entry name" value="SET binding factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051696">
        <property type="entry name" value="DENN_Domain_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022096">
        <property type="entry name" value="SBF1/SBF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296:SF16">
        <property type="entry name" value="C-MYC PROMOTER-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12335">
        <property type="entry name" value="SBF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03456">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00801">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00800">
        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="5">
        <location>
            <begin position="1"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Myotubularin phosphatase" evidence="6">
        <location>
            <begin position="968"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="1611"/>
            <end position="1715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1146"/>
            <end position="1166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1717" mass="194246" checksum="E9C940BDCAE37ACE" modified="2018-12-05" version="1">GTQLKDWLVLVTKGEGQGRILQRFPEKDWEDSPFPQGIELFCQPSGWQLVPERQPASFFVAVLTDINSERHYCACFTFWEGLDSPQVRGTPHSKLFAPKSLVVVSRLDYTEAFRNCLGLIYTVHVDGLSTPLETVIGNLLTCVIPVAGGSQRTITLGAGDRQVIQTPINDALPVSGSSVAQLFRQLGIVNVLYLFCAALTEHKILFLSSSYQRLTDACRGLLAIMFPLKYSFTYVPILPGKLLEVLSTPTPFIIGVNSFFRSETQELLDVIIADLDGGTVTIPECVHISLLPEPLLQQTQTALSMVLDPELEFSDQAFPPQSMNPSALKIQDKEIRAVFLWLFARLFQGYRWCLHIIRIHPEPVIRFHKAAFLGQRSLTEDDFLMKVLDGMAFAGFVSERGPPYRATDLFDDLVANHVERIRQEEACPHKVMNHVKELAEQLFKNENPYPAVAMHKVQRPSETSQSTDQSRTPFPVLDDVAVQLFIDHAVAKLKSSPPVVKAELKSMVPSGPPLGENGNVMANSARRLEVVRNCITYIFDNKMLEAKKLMPAVLRALKGRTARVCLTQELNQHVLQNRAVLDDQQFDYIVRMMNCTLQDCSHMDEHGIAATLLPLVTAFCRKLGAGITQFAYSCVQEHMVWTTMQFWEAMFYSDVQNHIKALYMETDDGNQDQQDGSSNRREISALELAAEQNRLWPTLSKEMQMERVQKEESTVFSQAIHYANRMSYLLLPLDTSKNRLLRSSGLGDVESVSNSYVTNSIAGSMAESYDTESGFEDAESSDVANSVVRFINRFVDKVCNESGVTNEHLKALHTMIPDIVQMHIETLDAVHRESKRLPPIQKVTNSCFYLPLEKGHAEPHPAPPTPLSTNPFSSGSQCHGRYVCPSRQNGYVLILFRTLSKNLVKNAKRTISRQYVGRKKEVPATWENRSNFQSELDEDEISVSEEVDQSALTLSSTIRSSDRQTMSNVVERACCRDYQRLGLGTLSNSLTRSKNEPFRISTANRMYTVCRSYPGLLIIPQNIPDSTIHRICRCYRQNRFPVVCWRHSRTKAVLLRSAGLHAKGVVSFFKSPNAPTTVPSQADSTSLEQEKYLQAIISSMPSYSESTGRNTLSGFTSTHMNTSGQNSPRGKWGSIRGSGRLSAYNPDIGTRLAGKESPQPNGGPNDPLFLRQQKAYLYIIGDKAQLKGGKQDSFQQWEVIPIEVCDVRQVKNSFKKLMKACVPSSQTTEPNMSFLRLLEDSEWMTLLHRVLQVSVLVVELLDTGSSVMVSLEDGWDVTTQVVSLVQLLSDPYYRTFDGFRLLVEKEWLSFGHRFSHRGAQTLGSQSSGFTPVFLQFLDCVHQIHLQFPMEFEFSQYYLKFLAYHYMSNRFRTFLLDSDYDRIELGVLYEEKSDRRNPQVCKSVWDYIDRLNKKAPVFYNYMYSPEDEEVLRPYTFISNLKVWDFYLEETLSDGPPYDWELRSRQESLAEETAEKADTGAPKSQRHIVWPCYDNLSKVMPDAITKLLQDLQNLEAELGQTSEKWKDTWDKIKTTQRTETKLESKPSFSSSLLMSSNLSHQRRSQGVCLQDSRVGSSIKLGLDCEASATSTPVAGRPSTSTLYSQFQSTESENRSFEGILYKRGALLKPWKPRWFVLDKTKHELRYYDSRQDKDCKGVIELSDVESVTVGTPTMGAPKNVEEKAFFDLKTSKRMYNLCAQESSNAQLWMDSIQSSLSDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXQ8</accession>
    <name>A0A3B5KXQ8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CALHM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXQ8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002815"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002852.1">
        <property type="protein sequence ID" value="ENSXCOP00000002815.1"/>
        <property type="gene ID" value="ENSXCOG00000002237.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234610"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029569">
        <property type="entry name" value="CALHM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32261">
        <property type="entry name" value="CALCIUM HOMEOSTASIS MODULATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32261:SF4">
        <property type="entry name" value="CALCIUM HOMEOSTASIS MODULATOR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14798">
        <property type="entry name" value="Ca_hom_mod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="181" mass="20331" checksum="B853F4DB957966D0" modified="2018-12-05" version="1">MDRFKTILNIASKQTNLGFGLVALLTAGGEQIFSSVAFKCPCNELNFLYGLVFLLVPALALLLLGYILSKKMWILFTGLWHNRAKLCYWKNLATTCTAFLQISSTALVAPSSWLAVALLNGNYYECAMTGTNLSVYNQYLCKDMNFELDCGKKLHMLPCDKRNEEVLRTLRSQSQVSSFLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KY62</accession>
    <name>A0A3B5KY62_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Metalloendopeptidase</fullName>
            <ecNumber evidence="2">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1 2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KY62"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003002.1">
        <property type="protein sequence ID" value="ENSXCOP00000002965.1"/>
        <property type="gene ID" value="ENSXCOG00000002342.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163716"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127:SF899">
        <property type="entry name" value="ASTACIN-LIKE METALLOENDOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10127">
        <property type="entry name" value="DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 2" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12A" evidence="4">
        <location>
            <begin position="93"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="295" mass="34679" checksum="F05AD71154DCD3B7" modified="2018-12-05" version="1">MPSLHKSGGILSVNNSSTKYNKRKGKITMTQDKISNKSRQNSYITAIILQLHELKAKENKLVFRRDLKSPKLLEENKPKRYDDIAMPKIFKRNADPCTIVGCKWFKSGEHVYIPYYIDTNYSPEERHQILAVLQRFNKSTCIRFVPFKLKNSDYLYFYTRPNEGCWSYIGRQGGGQFISLERNKCLRTSTVSHEVLHALGFNHEQVRIDRDEYIRVLFQNIKPEQRFNFWKVRTNNMGSPYDYNSVMHYKKYAFSKNGQPTIAARSHQIHSFGNSYGMSNTDIDRVNKLYNCRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZT1</accession>
    <name>A0A3B5KZT1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Nucleobindin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">NUCB2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the nucleobindin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZT1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001069"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001082.1">
        <property type="protein sequence ID" value="ENSXCOP00000001069.1"/>
        <property type="gene ID" value="ENSXCOG00000000867.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001927"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000045">
        <property type="entry name" value="Nucleobindin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057576">
        <property type="entry name" value="NUCB1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040250">
        <property type="entry name" value="Nucleobindin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19237">
        <property type="entry name" value="NUCLEOBINDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19237:SF23">
        <property type="entry name" value="NUCLEOBINDIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25434">
        <property type="entry name" value="NUCB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="11 19" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017352259" evidence="19">
        <location>
            <begin position="24"/>
            <end position="415"/>
        </location>
    </feature>
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        <location>
            <begin position="240"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="20">
        <location>
            <begin position="292"/>
            <end position="327"/>
        </location>
    </feature>
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        <location>
            <begin position="182"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="346"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="182"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
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    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="415" mass="49618" checksum="7E1D075D9999B4F1" modified="2018-12-05" version="1">MVRSRALAHCGLVMLSLWLCVHSVPISVEKAKDKPEVEELEPPQSDTGLHYDRYLREVIEYLEKDPHFREKLKNANMDDIKQGKLSKELDFVQHNFRSKLDELKREEMNRLRLLIKAKHDLREGNGNTLDHQALLKQFEHLNHMNPHTFEVEDLDRLIQSATKDLENYDKERHDEFKQYEMTKEHERRERLKTMNEEDRKKEEQHYEEMKKKHANHPKVNHPGSEDQLKEVWQESDGLDPEDFDPKTFFKMHDSNGDGFFDESELEALFTKELEKVYNPENEEDDMVEMEEERLRMREHVMNEVDTNKDRLVSMSEFIAATKKAEFHEKDEWGTLDQNPAYTEEELREYEQQIANEEDNINTKTAELQKQREELERKQEELNAQKSGLKQVSYCCESAKKKGKRERSCIFIFFVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L0N3</accession>
    <name>A0A3B5L0N3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L0N3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003922.1">
        <property type="protein sequence ID" value="ENSXCOP00000003880.1"/>
        <property type="gene ID" value="ENSXCOG00000003047.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000182110"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="30" mass="3608" checksum="F7B16100A7633B7B" modified="2018-12-05" version="1">YEEKLNLCKSFLIYNNSQTNLFQQTLLLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1H5</accession>
    <name>A0A3B5L1H5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methylosome protein WDR77</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Methylosome protein 50</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">WD repeat-containing protein 77</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1H5"/>
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    <dbReference type="Ensembl" id="ENSXCOT00000004704.1">
        <property type="protein sequence ID" value="ENSXCOP00000004647.1"/>
        <property type="gene ID" value="ENSXCOG00000003670.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:methylosome"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="P:oocyte axis specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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    </dbReference>
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        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46853">
        <property type="entry name" value="METHYLOSOME PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46853:SF1">
        <property type="entry name" value="METHYLOSOME PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="111"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="153"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="326" mass="35898" checksum="51D3BF2ED138C86A" modified="2018-12-05" version="1">MIKENPWKIPPNAPACMEKHLCAAQYRADGTLLLGASSLSGRNWQGSVWIYSETEQAPSEGFCKAGVQTEAGVTDLQWVAEKGVAVASDSGALELWELAEDERLLMNRFTKYEHDHIVTTVSPITGASSAVTGSMDCRIKVWDLSQETVVTTYNVHTEPVNCVACSPTDESLFVSCGQDGRVLMWDKRKPDKPASRIDVEFPSCSPTTAVWHPHQRSTIAFGDEMGNVSVKDFLGSEPARVKKIHSRRVNSLAFSTNSVPLLASVSDDCSLAVINSELQEIFRDRRHQDFAKGVCWLHGDSNRLTTVGWDHLVLHHTIAPNTESSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L357</accession>
    <name>A0A3B5L357_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L357"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004291"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004338.1">
        <property type="protein sequence ID" value="ENSXCOP00000004291.1"/>
        <property type="gene ID" value="ENSXCOG00000003370.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002461"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000856">
        <property type="entry name" value="ER membrane protein complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058545">
        <property type="entry name" value="Beta-prop_EMC1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026895">
        <property type="entry name" value="EMC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011678">
        <property type="entry name" value="EMC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573:SF0">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25293">
        <property type="entry name" value="Beta-prop_EMC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07774">
        <property type="entry name" value="EMC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017443186" description="ER membrane protein complex subunit 1" evidence="12">
        <location>
            <begin position="21"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="domain" description="EMC1 first beta-propeller" evidence="14">
        <location>
            <begin position="20"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 1 C-terminal" evidence="13">
        <location>
            <begin position="763"/>
            <end position="968"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF25293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="969" mass="109213" checksum="EA3C9CE8997227A6" modified="2018-12-05" version="1">MAKLILLLLKLCILFFVVDAVFEDQVGKFDWRQQYVGKVRFSHFDTQLQSSKKVLVATESNVFAALNSRTGELFWRHVDKTGPEGNIDALLHHAQDAVLVVGNGRMLRSWDVNVGGLNWEMLLDTGSFQSACLVGQQGTVKHVAVLKKTVISVHYLSNGHQKWVENLPESETVDYQTVYSGGNGQVFVLGVVPHSHITIVVYSMEDGEIIKQVSVEAPWLSNIPASCAVVGQGTLMCVDSTTMSLNTLDLHQQLQMTQIPLQSLGLEVDPDFHPSLVSHQPNPAQKPLSEFFLQLGPERYLLLQLNNGQIVTLRDFKPALLSSFVTTGEKTVAAVMSPKNKTVSSYRIGLSTHSGINLFSSDTGRRLLDTTLIFTVDPNGGKPERLHVQAFLKKDDSVGYRVMVQTEDHTLTFIQQPGRVMWTREEALSDVVTMEMVDLPLTGTQAELEGEFGKKADGLLSMVLKRLSSQLIMLQAWIAHLWKLFYDARKPRSQVKNEVSIENLSRDEFNLQKMMVMVTSSGKLFGIDSKTGEILWKHYLDNVPKNAAFKLMVQRTTAHFPHPPQCTLLIKDKDTGLATLHVFNPIFGRKSQVTPPALPQPILQSLLLPLMDQDYAKVLLLVDNQYKVSAFPSTKNVLQQLQETASSIFFFLIDSSQGRLSGYRLRTDLSTEQVWEVVIPTEVQKIVSVKGKRPNEHVHSQGRHFLHNLIYLNPNLLAVVTESTDVHQERSFIGILLIDGVTGRIIHEAVQRKARGPVHVVHSENWVVYEYWSTKSRRNEFSVIELYEGMELYNSTVFSSLDRPHAPQVLQQSYIFPSSISTMEATLTEKGITSRHLLIGLPSGGILSLPKMFLDPRRPEIISEQSREENLIPYSPELIIRTEWFINYNQTVGRVRGIHTTPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYMLISSVLFALFFATMISKRLAEVKLLNRAWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5A9</accession>
    <name>A0A3B5L5A9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5A9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005656.1">
        <property type="protein sequence ID" value="ENSXCOP00000005590.1"/>
        <property type="gene ID" value="ENSXCOG00000004361.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000178331"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007288">
        <property type="term" value="P:sperm axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038905">
        <property type="entry name" value="ARMC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21356">
        <property type="entry name" value="ARMADILLO REPEAT CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21356:SF1">
        <property type="entry name" value="ARMADILLO REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="108" mass="12370" checksum="A8FC4CB7E081D49C" modified="2018-12-05" version="1">METKHEVCSAFTPRRNSLRKSCADIIREARQSLRVQSTQRPFTPRDGPRRLFGANSVRRDHGSRPPSAFRLGWHGLGPASSSLHLLHHCCLQKHAQMVRKEKRFVSTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6M0</accession>
    <name>A0A3B5L6M0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pleckstrin homology domain containing, family N member 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6M0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006085"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006155.1">
        <property type="protein sequence ID" value="ENSXCOP00000006085.1"/>
        <property type="gene ID" value="ENSXCOG00000004710.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155360"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901612">
        <property type="term" value="F:cardiolipin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070300">
        <property type="term" value="F:phosphatidic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901981">
        <property type="term" value="F:phosphatidylinositol phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061158">
        <property type="term" value="P:3'-UTR-mediated mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042835">
        <property type="entry name" value="PLEKHN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46882">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY N MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46882:SF1">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY N MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="88"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="361"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="618"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="620"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="687" mass="77966" checksum="F049F2DBFCA157AC" modified="2018-12-05" version="1">MGSSISCVPQHNFRYSSKSFIRRNSSRLFRKKNPQEGQEKSNSIINILCTVTPRKEMSPKDLQTIENIKWDPPFAYDPASGWKKSSINVKNFGRMIHSSKVRFRFLHCQDIHDCYLDLFESHLHFVSNNTTGLTYQGTLPLKELSICNLQQNCNHSQPEEYAFQINGVSLNPIIVYCASQDEMDLWFGLLKESIEQNGGTAIQPENYTRVRNKSEGREELRNSINREPIYEWEGSQRDSLGPIAYVTKVRLQHLPCQVQSDRLLVMYPSTLIILSEESNGLFYKGKLPLNMITVTTPCQDVKPNTFMIEGKLINPIVVSCLDRTEFCDWIQQFKAVDVPVLSPPPPVYDIIYTPTNKEQATKFDRWSGNSQGRAESLKYSQPPSGRGSLNLQVPVHEENLMSPGYSEPLCYSSSRPSSIETARLGSRTNSVSSQTRPDRDPRPLSLRYSSPQHGSYLQPAESAPLSQLYSTPYSALHRVSPQRLEKAPLVKSNSWSTPQTSLNYSLSASQRHSDLCALRQPLSPLYDEPCTPEIFSLEEPRPKSWNKPIQHNHQDPNLLPSSFQLRTPPMGRRTGRNLALANTQAQVSGLHVEMPQSQAEQRAEAVSRLKLLPMPGHVQEQVRQETDRHSYLEPMDPDDQEVDYDNIWEYDGETGMIQPVCGISTQWTGLDFGAQGLGPMATRQKWC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6W6</accession>
    <name>A0A3B5L6W6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bcr-Abl oncoprotein oligomerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
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    <sequence length="124" mass="13853" checksum="D314C537755E0FF9" modified="2018-12-05" version="1">MTEQLSPDSEAPPRDEEAESNHVGQEAETLVQTEDVGITDSESPLGVVSNGDKHEDPVAPYVNGCKVDREKAQRLAEQLFKLDNIQRSDVVKHLDKDNDFSHAVGEEYLKFFDFSGQTLDHALR</sequence>
</entry>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="18068" checksum="9319FE5575939DA3" modified="2018-12-05" version="1">MSGNNPEGRTVEVLELPENVDEELLSLYFENRRRSGGGPLESVEIKQNRAVLCFEDAEAAARVLSKRHHVVHNSELSVRKPPSKDQSRLLLRGVSPYTSTEMIELYVENMMDLNVTDYALIPSPEKDFILIHLSQPLSKGRMGVFFTSVQKTFRDDAYK</sequence>
</entry>
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    <accession>A0A3B5LB17</accession>
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        <submittedName>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="2">
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
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        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptotagmin family.</text>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:negative regulation of secretion by cell"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
    <dbReference type="CDD" id="cd08404">
        <property type="entry name" value="C2B_Synaptotagmin-4"/>
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    </dbReference>
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        <property type="entry name" value="Synaptotagmin 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000051">
        <property type="entry name" value="Synaptotagmin 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024">
        <property type="entry name" value="SYNAPTOTAGMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10024:SF114">
        <property type="entry name" value="SYNAPTOTAGMIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="196"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="14">
        <location>
            <begin position="330"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="129"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="170"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="472" mass="52790" checksum="0720B40251BE61D3" modified="2018-12-05" version="1">MLLCFKVCFLIKSYPFLFTFFLFFFLYLSPNSSAVAVPVGVAVVSVFGLVFTASAFAWICCQRKNTNKSQKTPPYKFVHMLKGVDIYPESLSGKKKFAAATTSTANDTAKTDVNGNSLHLDLEKRDLNGNFTTKPFHHHHQTVRSSPDLELPSPQTGFTQPGAMERRDLPSPSSALSSQAPTPAVDRPQGDEKEGGLGTLHFSLEYQAERKAFIVHIKEAHGLTPTDEQSLTSDPYIKLTLLPEKKHRVKTRVLRKTLDPAFDETFSFYGIPLARVSELALHFMVLSFDRFSRDEVIGETLVPLSGIDLSEGRVLMSREIIKRNVKKSLGRGELLLSLCYQSTTNTLTVVVLKARHLPKTENNGPTDPYVKVNMYQGKKRICKKKTHVKKCSPNPVFNELFVFDLPSEEGLRDTSVELLLMDSDNGTSRNPNTVIGRLVLGTSAAGTAGEHWREICDHPRRQIAKWHTMSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBU1</accession>
    <name>A0A3B5LBU1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBU1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005400.1">
        <property type="protein sequence ID" value="ENSXCOP00000005339.1"/>
        <property type="gene ID" value="ENSXCOG00000004179.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286924"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46484:SF1">
        <property type="entry name" value="SCHWANN CELL MYELIN PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46484">
        <property type="entry name" value="SI:CH211-171H4.5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="157"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="288" mass="33373" checksum="7BDF0703623AA1B2" modified="2018-12-05" version="1">PAIFSYHVSPWVMLWDLSRISGSGKDWLIDVPSRVSVLQNSCVVIPCSYVYPKVRKMLKTWRGYWKRRGTVVASNFPNAKLTDEFHHRSRIRGTLQSGNCTWQLNRVRETDTGPFYFKIEIPQHKTFTFSQNTVTLDVFREYIRGLFTNSKPFTLRLTATCKVTHVCPSSPPKFSWSHSGTRKTNLQKTVSTLTFTASAKDNGKSLTCYATFPGGQTQEKSMTIRVKREFVPFCFYVFFRKHAQSGLVFHYSRKRNRNERIMCHHSLHVFLQQRQTGWPGSCLVLVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCH5</accession>
    <name>A0A3B5LCH5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">type I protein arginine methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.319</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48096"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11991"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61897"/>
            <dbReference type="EC" id="2.1.1.319"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:48097"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:48101"/>
        </physiologicalReaction>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCH5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009883"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010001.1">
        <property type="protein sequence ID" value="ENSXCOP00000009883.1"/>
        <property type="gene ID" value="ENSXCOG00000007498.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155867"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035242">
        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035241">
        <property type="term" value="F:protein-arginine omega-N monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.160.11:FF:000001">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000003">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF47">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="9">
        <location>
            <begin position="241"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="36"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="418" mass="47725" checksum="811EB84FF376DE8F" modified="2018-12-05" version="1">MNSSKGCLLKENLFSVLKKRLSSSLVFLKQPQSQPRPVRIIPSQSAQPTSLPKPVPSVQPASRPALPPHTPHAAGLPSCPGRGKMAKFLSPDEMTSRDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMYHNKHIFKDKVVLDVGSGTGILSMFAAKAGAKHVYGIECSSISEYSERIIKSNHLDSVITIFKGKVEEVELPVEKVDIIISEWMGYSLFYESMLNTVIFARDKWLKAGGLMFPDRASLYVLAIEDRQYKDFKIHWWENVYGFDMTCIRNVAMKEPLVDVVDPKQVVTNSCLVKEVDIYTVKPDDLSFTSAFCLQIQRNDYIHALVTYFNIEFTKCHKKTGFSTAPDAAFTHWKQTVFYLEDYLTVRRGEEIAGSITMKPNEKNIRDLDFTFELDFKGQLCEAAISHDYKMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDH2</accession>
    <name>A0A3B5LDH2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Motilin receptor</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDH2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007741"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007838.1">
        <property type="protein sequence ID" value="ENSXCOP00000007741.1"/>
        <property type="gene ID" value="ENSXCOG00000005949.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271823"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15132">
        <property type="entry name" value="7tmA_motilin_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003905">
        <property type="entry name" value="GHS-R/MTLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243:SF3">
        <property type="entry name" value="MOTILIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01417">
        <property type="entry name" value="GHSRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="306" mass="35299" checksum="AAD906F75FA559A3" modified="2018-12-05" version="1">MTILIIQHFKDMKTTTNLYLSSMAVSDLIIFLCLPFDLYRLWKYVPWVFGEAVCRFYHYIFEGCTSATILHITALSIERYLAISFPLRSKVMVTRRRVQYIILALWGFALVSAAPTLFLVGVEYDNDTHPDYDKGQCKHTSYAISSGQLHIMLWVSTTYFFCPMLCLVFLYGSIGCKLWKSKNDMQGPCTLARERSHRQTVKILVVVVLAFIICWLPYHIGRNLFAQVDDYETAMLSQNFNMASMVLCYLSASINPVVYNLMSRKYRAAAKRLFLLHQRPRQAGRSQRQLCMIDHISTLNESLTGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LF54</accession>
    <name>A0A3B5LF54_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMCO4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LF54"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010923.1">
        <property type="protein sequence ID" value="ENSXCOP00000010798.1"/>
        <property type="gene ID" value="ENSXCOG00000008149.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001400"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007941">
        <property type="entry name" value="DUF726"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920:SF3">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4 TMCO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05277">
        <property type="entry name" value="DUF726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="514"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="588"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="514"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="599"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="608" mass="66227" checksum="FD17B374511BD84D" modified="2018-12-05" version="1">PEKITSRQLTEQSRFAFAALCAVSLGQLFSAFREQYLQDLVQWLGLDQSVMPVMGAFLSGLGSEGTDTFLSILQADPLVAAGATPIIQDLVSLSVKDQYDARARVLIRHVSCLLRVSPQQLEEFEETLGERLREAGEESREESSRRQRRERKRKLRRYLLIGLATVGGGTMIVTGGLAAPLVAAGAGAVLGAGGAAALGSAAGIAIMASLFGAAGAGLTYKMNKCVGAIEEFEFRPMSSGKHLHLTIAVTGWLCSGKYSYQAPWSSLGECGEQYCLVWESRFLRDLGSAMISLLDGLVSMVAQEALKYTVLSIVTALTWPASLLAVASIIDNPWCVCLNRSAEVGKHLAQVLRSRQQGKRPVSLIGFSLGARVIYYCLQELASDKSEGVVEDVVLLGAPVDGSEKSWERMVKVVAGRIVNGYCGDWLLGFLYRSSAAQMSVAGLQPINIQDRRIINVDLSSVQVKGHLDYMRQMDTILVAVGVPTKEVAGASFVLPQSVRVAQKTVDNLDWAEEQHRASETQNPAEKDKTSSQDWRDETEDMGNGWDIPDITDLLDSLNETEQNNGVSVLNIVSPNDTTSSDFIAQNSREEAEPDDEQISWSWNDTHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFG7</accession>
    <name>A0A3B5LFG7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zgc:152830</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M17 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFG7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006614"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006695.1">
        <property type="protein sequence ID" value="ENSXCOP00000006614.1"/>
        <property type="gene ID" value="ENSXCOG00000005117.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063255"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00433">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963:SF48">
        <property type="entry name" value="DIPEPTIDASE B, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="LEUCINE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytosol aminopeptidase" evidence="5">
        <location>
            <begin position="345"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="517" mass="55749" checksum="3C784B85C38E4347" modified="2018-12-05" version="1">SLSSVQPTEWTADLKNQNFDGIILVTQSHDALPAELESLKAPLQDYSSVDSGLGDEVVLIKVPSLPGNRLIFASTGPVNRDYDDVRRFSDAAVKGIKRALKAGMQRPLLVCPPHKDYKENALAAALGALHALYMPLEVREANIKSSVHKVCVLGLWAAQEAEGRRLVELANALESGRLAYRDIGGSDPERMAAPRVAEYVQELFKNSPVEVEVLSSVKTLESEYPCLAAVNRCANNVPRHQARVIKLQYCGEGPIQKTLMLVGKGITYDTGGADIKAGGIMAGMHRDKCGAAAVAGFFQILAHLKPRHLKVFGAMTMVRNSVGSDCYVADELVVSRAGRRVRVGNTDAEGRMVMVDLLCEMKEKAAQEVSPHIFTIATLTGHAIRAMGSNYSIIMDNGPAHRNGNAAQWQKAGDVLGDVFEISNIRREDYEFHKGPSEYEDILQSNNLPSSATPRGHQTPAAFLIMTSGLDKHGVDSDAPLPYSHVDIAGSSGPFPGVPTGAPIVAMAANYIFQDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFX3</accession>
    <name>A0A3B5LFX3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FERM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFX3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009490"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009602.1">
        <property type="protein sequence ID" value="ENSXCOP00000009490.1"/>
        <property type="gene ID" value="ENSXCOG00000007224.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158972"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13193">
        <property type="entry name" value="FERM_C_FARP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000002">
        <property type="entry name" value="Band 4.1-like protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000040">
        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000005">
        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041788">
        <property type="entry name" value="FARP1/FARP2/FRMD7_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051835">
        <property type="entry name" value="RAC1-GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45858">
        <property type="entry name" value="FERM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45858:SF1">
        <property type="entry name" value="FERM DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="1">
        <location>
            <begin position="2"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="297" mass="34579" checksum="D3C6B0A2EFC918CA" modified="2018-12-05" version="1">KLRLRVIFLDDSERTFEVEQNVLGGDFFNKVCGHLKLLEKEYFGLEFRHHSGHYVWLELLKPLVKQIKTNDLFFRFIVKFFPPDPGQLKRGLTYLFALQIKQDLSNGSLTCNDNSAALLVSHILQEIGDYDEELDYQHLEMKHYIPNQEYLDNKIIKLHKRHGVSPADSDIQLLEVARKLDMYGIRPHAANDGEGMRINLAVTHSGVLVFQGNTKINTFSWAKIRKLSFKRKHFLIKLHDKVGASCKDTLEFAMASRDVCKLFWKMCVEYHAFFKLAEEPKPINKTLLSCKGSRFRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LG76</accession>
    <name>A0A3B5LG76_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LG76"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011276.1">
        <property type="protein sequence ID" value="ENSXCOP00000011148.1"/>
        <property type="gene ID" value="ENSXCOG00000008427.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244823"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15055">
        <property type="entry name" value="7tmA_TAARs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000318">
        <property type="entry name" value="Trace amine associated receptor 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF381">
        <property type="entry name" value="TRACE AMINE ASSOCIATED RECEPTOR 19P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="46"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="327" mass="37453" checksum="2C3C64F609AF966B" modified="2018-12-05" version="1">MEIQDRTDLCFPHLLNSSCRKPTLHWSEAILLNSVLLFISLITVVLNLLIIISVSHFRQLHTPTNILLLSLGVSDFFVGLLLMPFEIYRFTFCWFLGDAMCALFWFLMCNLISASIWNIVLVSVDRYIAICYPLDYSRKMSLMRVKYCVCLCWFCASSCSFFYAKDELIQSGRSKSCIGECKFIMSYIPGTFDLIFNFILPVTTIIVLYLRVFVVAVSQARAMRSHITVASFHSATSGTKKSELKAARTLGVLVVVYLMCYCPYYCYSLVGISLTNTPYSFSMFFLCYVNSCLNPVIYALFYPWFRKAVKVIVTLQILLLKIKKARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LG78</accession>
    <name>A0A3B5LG78_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cadherin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LG78"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009713.1">
        <property type="protein sequence ID" value="ENSXCOP00000009600.1"/>
        <property type="gene ID" value="ENSXCOG00000007308.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165759"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000004">
        <property type="entry name" value="Protocadherin 1 gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000006">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000129">
        <property type="entry name" value="protocadherin alpha-C2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032455">
        <property type="entry name" value="Cadherin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF296">
        <property type="entry name" value="PROTOCADHERIN 1 GAMMA 11 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16492">
        <property type="entry name" value="Cadherin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017307137" description="Cadherin domain-containing protein" evidence="15">
        <location>
            <begin position="35"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="690"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="32"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="139"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="248"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="351"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="456"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="580"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="799"/>
            <end position="838"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="838" mass="92516" checksum="9768AD76343D3E5F" modified="2018-12-05" version="1">METHQHRCGKWLPRLRTPLWQLFLVLLMAFSVDGQIRYSIPEEMKKGSLIGNVAQDLGLDLSRLRSGRARIVSGESNQYTELKADKGILVVNENIDREQLCGEVTPCSFSFEVILENPIELHRVTVEILDVNDHAPTFKNKAINLEISESATIGSHFDLESAYDPDAGIYSLQNYVLSPNDHFALKQHSNADGVKFPVMILQKSLDRELNAHVSLKLIAVDGGTPQRSGTVNIEINVLDVNDNPPKFSQLVYKAVVVENAPIGTYITTVNASDADSGSDGLVTYSFSNVKRNFAFKIDSLSGRITVVDSIDYEKDKKYELRVIAKDQGGLTDATKVTIDVLDANDNSPVISIMSFSNTVSEDAPPGTTVGVFNVKDADSDRNGEIKCSIESNFPFKIQSSLTDYYNLLLDASLDRETTPEYNITITAMDHGSPSLSSSAVLYLKVSDVNDNPPIFENPNYSAVIIENNNPGVSVFSMSARDSDWNQNAKISYFLEDTVVSGSPVSSYVSINSESGVIQAVRSFDYEQIKQLVFTVRAQDGGSPPLSSNVTVKILIQDQNDNPPQVLYPVQTGGSMVAEMVPRSADVGYLVTKVVAVDVDSGQNAWLSYKLQKATDRALFEVGLQNGEIRTIRQVTDKDAVKQRLTVIVEDNGQPSRSATVIVNVAVADSFPEVLSEFSDFPHDKEYNDNLTFYLVLALAVVSFLFITCLVVIISVKIYRWRQSRILYHSSLPVIPYYPPRYSDTMGTGTLPHVYNYEVCRTTDSRKSDTKFGGAGSQNVLIMDPSSTGTMQRILCEKSILDEPDSPLESRWSPGLPSEYRRPPGLPPGLHSKSRWSPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LG80</accession>
    <name>A0A3B5LG80_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027895511.1">
        <property type="nucleotide sequence ID" value="XM_028039710.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027895512.1">
        <property type="nucleotide sequence ID" value="XM_028039711.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027895513.1">
        <property type="nucleotide sequence ID" value="XM_028039712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LG80"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009975.1">
        <property type="protein sequence ID" value="ENSXCOP00000009857.1"/>
        <property type="gene ID" value="ENSXCOG00000007484.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114158336"/>
    <dbReference type="KEGG" id="xco:114158336"/>
    <dbReference type="GeneTree" id="ENSGT00630000089942"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46829">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46829:SF1">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="2">
        <location>
            <begin position="6"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="141" mass="16661" checksum="E42F3ED2778DC5D0" modified="2018-12-05" version="1">MEFLNWDTEDVANWVESIGFSKYKDCFTENFINGKKLIHVNCNNLPKLGVTDFRDILAISAYVRELLEITETPWNNSIADPRRDTMALFLEVKSRTGRWTNSLTYQQFLYYLEDPDAEPEDDENKDENEDEDDFDDEENQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LGV8</accession>
    <name>A0A3B5LGV8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="18">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + 4 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 4 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56008"/>
            <dbReference type="Rhea" id="RHEA-COMP:14356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:139493"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + 3 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 3 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56028"/>
            <dbReference type="Rhea" id="RHEA-COMP:14358"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60628"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endomembrane system</location>
            <topology evidence="14">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LGV8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010092"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010211.1">
        <property type="protein sequence ID" value="ENSXCOP00000010092.1"/>
        <property type="gene ID" value="ENSXCOG00000007657.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159312"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000002">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF28">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE 1,2-ALPHA-MANNOSIDASE IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 18" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="15">
        <location>
            <position position="316"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="270"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00060399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="452" mass="51293" checksum="D6093735773C20D1" modified="2018-12-05" version="1">MMKFAWDNYKRYAWGKNELRPLTKNGHLGNMFGGLRGASIVDSLDTLYIMGLMDEYNDAKEWVQTSLDLNSNGEASLFEVNIRYVGGLLSAYYLTGEEFFRNKAVELGEKLLPAFNTPTGIPRGVINLGSWGWASAGSSILAEFGTLHLEFVHLTELSNNPVFKEKVMSIRKLLNKIEKPHGLYPNFLSPVSGNWVQHHVSIGGLGDSFYEYLIKSYLMSDKTDEEAKKMYYSALEAIEANLVQKSPGGLTYMAEWRGGILDHKMGHLACFSGGMIGIGADDGAPEKRQHYLDLAAEITHTCHESYTRSTTRLGPEAFRFDSGAEATATRLSDRYYILRPEVIESYMYMWRLTHDPKYRDWGWEAVEALEQHCRVESGFSGIRDVYALTASHDNMQQSFFLSETLKYLYLLFSDDDLLSLEDWVFNTEAHPLPIIRKSCLQDEITEEKTVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LH20</accession>
    <name>A0A3B5LH20_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">Tyrosine-protein kinase receptor</fullName>
            <ecNumber evidence="28">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="33 34">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="33" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="28">Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="28"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LH20"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007164"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007256.1">
        <property type="protein sequence ID" value="ENSXCOP00000007164.1"/>
        <property type="gene ID" value="ENSXCOG00000005529.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155645"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043121">
        <property type="term" value="F:neurotrophin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005030">
        <property type="term" value="F:neurotrophin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990090">
        <property type="term" value="P:cellular response to nerve growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010976">
        <property type="term" value="P:positive regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05094">
        <property type="entry name" value="PTKc_TrkC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000034">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000033">
        <property type="entry name" value="Tyrosine-protein kinase receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020777">
        <property type="entry name" value="NTRK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031635">
        <property type="entry name" value="NTRK_LRRCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002011">
        <property type="entry name" value="Tyr_kinase_rcpt_2_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF66">
        <property type="entry name" value="NT-3 GROWTH FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16920">
        <property type="entry name" value="LRRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01939">
        <property type="entry name" value="NTKRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00239">
        <property type="entry name" value="RECEPTOR_TYR_KIN_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 25 27" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13" id="KW-0221">Differentiation</keyword>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="22" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="17 29" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="11 25 27" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 28" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20 28" id="KW-0675">Receptor</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 30" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 28 29" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 29" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="18" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="30">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017232438" description="Tyrosine-protein kinase receptor" evidence="30">
        <location>
            <begin position="43"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="29">
        <location>
            <begin position="408"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="32">
        <location>
            <begin position="197"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="31">
        <location>
            <begin position="516"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="24">
        <location>
            <position position="657"/>
        </location>
    </feature>
    <feature type="binding site" evidence="25">
        <location>
            <begin position="522"/>
            <end position="530"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="25 27">
        <location>
            <position position="550"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with SHC1" evidence="26">
        <location>
            <position position="494"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with PLCG1" evidence="26">
        <location>
            <position position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR620777-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR620777-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRSR" id="PIRSR620777-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="RuleBase" id="RU000312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="803" mass="90614" checksum="E15D3E07A878C751" modified="2018-12-05" version="1">MKDVSDPDTEVRRTSSFDQTVRICSRWKVFLLLVCLFHSSLSSLLDCPPTCTCSSTEIYCNKSDNSSISQLLSFHMAGAENMSESIVDLFQNITSIYLENWIGLKVLKDVDMELYPGLQRLLLILFGNMFSFVLICSDCCICRHLEGVVFSCGCEIRWLQLWQQRGEAGLSSQQLTCSTGHTHILLLDMNVTNCDLPVVSVSHSNLTVVEGDEVTVICNGSALPIPEVDWAVNGLHSITTQQVLYNSIHSITIILANVKREDNNFWLNCTANNIVGSANASVHLIVHFPPSIEKLKEPERRHDTCIEFTVRGSPHPSLRWFHEDKISHNEYIRQDMDIYNDDLEGCLLFKNPTHYNNGNYTLKATNYLGEVNRTVYGHFLEIRSEQKTLPFFPGTPTAETHKPLEDPFGVSMAVGLAVFACVILVVLFVLINKYGRRSKFIIKGPGAVMSGEENSASPLHHVNHGVISPCALDVGLDSVVIGMSRIPIIENPQYFRHGHNGTKPTTYVQHIKRRDIILKRELGEGAFGKVFLAECYNLSPTKDKMLVAVKTLKDPTLAARKDFQHEAELLTNLQHEHIVKFYGVCVDGDPLIMVFEYMKHGDLNKFLRAHGPDATILVDGQPLQTNGELGLSQMLHIASQIAGGMIYLASQHFVHRDLATRNCLVGNGLLVKIGDFGMSRDIYSTDYYRVGGHTMLPIRWMPPESIMYRKFTTESDVWSFGVILWEIFTYGKQPWFQLANNEVIECITQGRVLERPRVCPKEVYDIMLGCWQREPQQRLIIKDIQKKLLALMKSTPVYLDILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHD0</accession>
    <name>A0A3B5LHD0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">FAM20C golgi associated secretory pathway kinase a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM20 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHD0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011538"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011670.1">
        <property type="protein sequence ID" value="ENSXCOP00000011538.1"/>
        <property type="gene ID" value="ENSXCOG00000008712.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182951"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070166">
        <property type="term" value="P:enamel mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024869">
        <property type="entry name" value="FAM20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009581">
        <property type="entry name" value="FAM20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450">
        <property type="entry name" value="DENTIN MATRIX PROTEIN 4 PROTEIN FAM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12450:SF11">
        <property type="entry name" value="EXTRACELLULAR SERINE_THREONINE PROTEIN KINASE FAM20C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06702">
        <property type="entry name" value="Fam20C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="FAM20 C-terminal" evidence="11">
        <location>
            <begin position="326"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="41"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="87"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="431"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="362"/>
            <end position="365"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="436"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="451"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR624869-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="560" mass="64014" checksum="1A15F5C372E0BDED" modified="2018-12-05" version="1">IAMILFRKFRVLILMVFLVACTLHIMIDLLPKLEKRAAGADGEEGGCQCAQHPARDSQGWGKQRARSAAEAGWPNKHTLRILQDFSNEPSSNLTSHSLEKSAAGVDRAGDSRGRTGLSAKEHKPLIGDASVGRTASAQALSRLSALFEHPLYKVDLPPLTDDDTLFNVNNDIRFYPRGNEDEDEFSPTAEATAESYPNWLRFHIGINRYELYPRHSPVLDALLKDLVTQRITSVAMKSGGTQLKLIMTFQNYGQALFKPMKQTREQETPPDFFYFSDFERHNAEIAAFHLDRILDFRRVPPVAGRLVNMTSEIRDVTRDKKLWRTFFISPANNVCFYGECSYYCSTEHALCGKPDQIEGSLAAFLPDLSLAKRKTWRNPWRRSYHKRKKAEWEVDPDYCDEVKQTPPYDRGTRLLDIMDMTIFDFLMGNMDRHHYETFEKFGNETFIIHLDNGRGFGKHSHDELSILVPLSQCCRVRKSTHLRLQLLAKEEYQLSSLMEESLLRDRLSPVLIQPHLLAMDRRLRLVLQVLTGCIDKEGYVNVVEEDLSGDTATHRGPAHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHE1</accession>
    <name>A0A3B5LHE1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fatty acid-binding protein, liver-type</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Fatty acid-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHE1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010277"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010400.1">
        <property type="protein sequence ID" value="ENSXCOP00000010277.1"/>
        <property type="gene ID" value="ENSXCOG00000007792.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157139"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000006">
        <property type="entry name" value="Fatty acid-binding protein, liver"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000463">
        <property type="entry name" value="Fatty_acid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031259">
        <property type="entry name" value="ILBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11955">
        <property type="entry name" value="FATTY ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14651">
        <property type="entry name" value="Lipocalin_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00178">
        <property type="entry name" value="FATTYACIDBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="133" mass="14989" checksum="94A7A03F344C17A8" modified="2018-12-05" version="1">MAFSGKWELESQEGYDAFCKFVLYFSLDELTFLLIYKGRDYKTVTEVVQSGNDFTWTQHYPSNAKVTNNFTIGKECDMQTIGGKSFKATVNMEGGKLTVSFPNYHQVNEVSGDKLIETSTAGTVVLKRISKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHK7</accession>
    <name>A0A3B5LHK7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fatty acid-binding protein, liver-type</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Fatty acid-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027867999.1">
        <property type="nucleotide sequence ID" value="XM_028012198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHK7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010085"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010204.1">
        <property type="protein sequence ID" value="ENSXCOP00000010085.1"/>
        <property type="gene ID" value="ENSXCOG00000007489.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114141582"/>
    <dbReference type="KEGG" id="xco:114141582"/>
    <dbReference type="CTD" id="171481"/>
    <dbReference type="GeneTree" id="ENSGT00940000164147"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000006">
        <property type="entry name" value="Fatty acid-binding protein, liver"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000463">
        <property type="entry name" value="Fatty_acid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031259">
        <property type="entry name" value="ILBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11955">
        <property type="entry name" value="FATTY ACID BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14651">
        <property type="entry name" value="Lipocalin_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00178">
        <property type="entry name" value="FATTYACIDBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00214">
        <property type="entry name" value="FABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytosolic fatty-acid binding proteins" evidence="8">
        <location>
            <begin position="5"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="126" mass="14103" checksum="7A3D65C27FBF22CE" modified="2018-12-05" version="1">MDLNGTWQVYSQENYEEFLRAMELPEDVIKMAKDIKPITEIKQTGNDFVITSKTPGKTVTNSFTIGKEAEITTMDGKKLKCIVNKEGGKLVCKTGKFNHTQELKGGEMIETLTVGSTTLVRKSRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJ68</accession>
    <name>A0A3B5LJ68_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-acetylneuraminic acid phosphatase</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJ68"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007924"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008022.1">
        <property type="protein sequence ID" value="ENSXCOP00000007924.1"/>
        <property type="gene ID" value="ENSXCOG00000006085.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003094"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050124">
        <property type="term" value="F:N-acylneuraminate-9-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046380">
        <property type="term" value="P:N-acetylneuraminate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04305">
        <property type="entry name" value="HAD_Neu5Ac-Pase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.710">
        <property type="entry name" value="Haloacid dehalogenase hydrolase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051400">
        <property type="entry name" value="HAD-like_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011950">
        <property type="entry name" value="HAD-SF_hydro_IA_CTE7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02253">
        <property type="entry name" value="CTE7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46470">
        <property type="entry name" value="N-ACYLNEURAMINATE-9-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46470:SF3">
        <property type="entry name" value="N-ACYLNEURAMINATE-9-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01135">
        <property type="entry name" value="C1.5.6:_HAD__Beta-PGM__Phospha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="243" mass="26873" checksum="EB53C190994C1CF9" modified="2018-12-05" version="1">MEGKAVKAILFDLDNTLVDTSRAGDVALKKVIKLLKSKLNLDDDTIRSICDKFKLKLLCESPDRPPGSSQDDIRMCHWTESIAETTGSSAASDLASQCYYLWKNSRLELLCLTPEVRDLLRQLSGRYKLLLLTNGDARTQREKVEAAECEEFFDAVVIAGDHAEQKPFPSIFQLCFSLLQVEAKDCVMVGDSLDTDIQGGVNAKVRATVWIHRADGSELDGPVKPDYTVPSVLDLPAVLAQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJD4</accession>
    <name>A0A3B5LJD4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Retinoid isomerohydrolase RPE65</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="4">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJD4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010770"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010895.1">
        <property type="protein sequence ID" value="ENSXCOP00000010770.1"/>
        <property type="gene ID" value="ENSXCOG00000008129.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182913"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052885">
        <property type="term" value="F:all-trans-retinyl-ester hydrolase, 11-cis retinol forming activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003834">
        <property type="term" value="F:beta-carotene 15,15'-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050251">
        <property type="term" value="F:retinol isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042574">
        <property type="term" value="P:retinal metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901827">
        <property type="term" value="P:zeaxanthin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543:SF43">
        <property type="entry name" value="ALL-TRANS-RETINYL ESTER 13-CIS ISOMEROHYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="BETA-CAROTENE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="481"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR604294-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="490" mass="56040" checksum="5EA96ABA83883504" modified="2018-12-05" version="1">VEELNEPISAEITGVFPLWLRGSLLRMGPGLFEIGDEPFHHLFDGQALIHKFDLKGDQVTYFRKFIRTDAYVRAMTENRVVITEFGTTAYPDPCKNIFSRFFTYFRGIEVTDNCLVNVYPIGEDFYAVTETNFITKVDPDSLETLKKVDLCKYLSINGVTAHPHVDADGSLYNIGNCFGKNMSLAYNIVKIPPAQKDKSDPIEKSQVVVQLPSSERLKPSYIHSFGMTENYFVFVEQPVKINLLKFLSSWSVRGATYMDCFESNENMGTWFHLATKDPADYMTNHKFRTSAFNLFHHINAYEDQGFIVVDLCTWKGHDFVYNYLYLANLRQEWEEVKKAAMRAPQPEVRRYVLPLDIHREEQGRNLVSLSYTTATAVLHSDGSIWLEPEVLFVTFCWFLPQIVKLNVQTKEMWVWQEEDCYPSEPLFVPTPGATKEDEGVLLSVVVKPGAERPGFLLVLDAMMLTELARAEVNTVIPVTLHGMYKPPPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJM8</accession>
    <name>A0A3B5LJM8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Platelet-derived growth factor (PDGF) family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the PDGF/VEGF growth factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJM8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009821"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009939.1">
        <property type="protein sequence ID" value="ENSXCOP00000009821.1"/>
        <property type="gene ID" value="ENSXCOG00000007455.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175612"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042056">
        <property type="term" value="F:chemoattractant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005172">
        <property type="term" value="F:vascular endothelial growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050930">
        <property type="term" value="P:induction of positive chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045766">
        <property type="term" value="P:positive regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001938">
        <property type="term" value="P:positive regulation of endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060754">
        <property type="term" value="P:positive regulation of mast cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002040">
        <property type="term" value="P:sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048010">
        <property type="term" value="P:vascular endothelial growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038084">
        <property type="term" value="P:vascular endothelial growth factor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000072">
        <property type="entry name" value="PDGF/VEGF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050507">
        <property type="entry name" value="PDGF/VEGF_growth_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12025">
        <property type="entry name" value="VASCULAR ENDOTHELIAL GROWTH FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12025:SF15">
        <property type="entry name" value="VASCULAR ENDOTHELIAL GROWTH FACTOR C-LIKE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00341">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00141">
        <property type="entry name" value="PDGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50278">
        <property type="entry name" value="PDGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 3" id="KW-0339">Growth factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Platelet-derived growth factor (PDGF) family profile" evidence="4">
        <location>
            <begin position="24"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="139" mass="15966" checksum="9C579C4FFDC8C09D" modified="2018-12-05" version="1">PTALLYSIYLLFHGEPSGSVRCQKTMGFREVLDKSFCRPIEQHVNVETEFPGGVEDFYIPHCVSLFRCSGCCIDETRECYPVVERNITLEVRSDHGRFQTQSITLKSSTHHASCEPSGKRFRSRVDSKLNWSLQQSEFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LL02</accession>
    <name>A0A3B5LL02_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase II N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027857738.1">
        <property type="nucleotide sequence ID" value="XM_028001937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LL02"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011607"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011739.1">
        <property type="protein sequence ID" value="ENSXCOP00000011607.1"/>
        <property type="gene ID" value="ENSXCOG00000008759.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114135029"/>
    <dbReference type="KEGG" id="xco:114135029"/>
    <dbReference type="CTD" id="26995"/>
    <dbReference type="GeneTree" id="ENSGT00940000156773"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02868">
        <property type="entry name" value="PseudoU_synth_hTruB2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039048">
        <property type="entry name" value="Trub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13195">
        <property type="entry name" value="PSEUDOURIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13195:SF0">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE TRUB2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pseudouridine synthase II N-terminal" evidence="3">
        <location>
            <begin position="91"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="295"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="309"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="336" mass="37584" checksum="ECCDB19F56D1B8D3" modified="2018-12-05" version="1">MRSPAVRMFHRLEGLFCVYKPAGVHWKLLRDTIETNLLKGVNDTPAPPLTHEVRFLPNPNNQPKASGGLMLRPVSVPVLSKHPLVTGPEFQHLKVGVGHRLDASSSGVLVLAVGNGNKILNDLYGIRTTRDYTLEGEFGMATHDFTHTGRVVERSTYDHITREKMESVLAMLEGANQKALRMYSSVDMNSQEAYELAVKGLLGPDGKSVPILTGLRCIRFQPPHFTLEVQCLNETQKYLRKVVHEIGLELRSTAACKGVRRTRDGYFTLQDALTHNHWSAADILQAVQKYRSKKNKKKSSRTLTDKAMLQTSEETLAVSQENKADKEETPTEVQCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLT7</accession>
    <name>A0A3B5LLT7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Potassium voltage-gated channel subfamily B member 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="28">Voltage-gated potassium channel subunit Kv2.1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="36 37">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="36" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="36" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <location evidence="2">Sarcolemma</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell projection</location>
            <location evidence="6">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lateral cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Perikaryon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="29">Postsynaptic cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="30">Synapse</location>
            <location evidence="30">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the potassium channel family. B (Shab) (TC 1.A.1.2) subfamily. Kv2.1/KCNB1 sub-subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027859594.1">
        <property type="nucleotide sequence ID" value="XM_028003793.1"/>
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    <keyword evidence="26" id="KW-0966">Cell projection</keyword>
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    <sequence length="836" mass="93928" checksum="335542845F758021" modified="2018-12-05" version="1">MLPQLKVIMEKSLDLNKAGSRSTSSLPPEPVDIIRSKSCYRRVRLNVGGLPHEVLWRTLDRLPRTRLGKLRDCNSHDSLMEVCDDYNLEENEYFFDRHPGAFTSILNFYRTGKLHMMEEMCALSFSQELDYWGIDEIYLESCCQARYHQKKEQMNEELKREADNLKDRDGEEFDNTCCAEKRKKLWDLLEKPSSSVAAKILAIISILFIVLSTIALSLNTLPELQGTDEFGQSTDNPQLAHVEAVCIAWFTMEYLLRFLSSPNKWKFFKGPLNVIDLLAILPYYVTIFLTESNKSVLQFQNVRRVVQIFRIMRILRILKLARHSTGLQSLGFTLRRSYNELGLLILFLAMGIMIFSSLVFFAEKDEEDTKFKSIPASFWWATITMTTVGYGDIYPKTLLGKIVGGLCCIAGVLVIALPIPIIVNNFSEFYKEQKRQEKALKRREALERAKRNGSIVSMNLKEAFARSAELMDVVVEKSERPHDNHLSSSHWKWASKRAASETSSNRSFNAQEIGSPCKARDTSPQRLNVEKLEEMYSQMAKTQSQPNLNAKDRGSRVSKQRDEIELGAIHDYGPSVLGAKDGVGDMKSLSSIDSYISCATDFQENPRYPHSPAADIGLKESTRFTFGPVTVLSQHISKKIGHQIIGQHESVLSPGLKSSGPENTRRFLFSGNSSKSLKGGCRSDGDSGPSPLSDSSVVSDQSLLSPEASVYTTASSRTPPKSPDSTAMAPTVFTFHDSSISKYIDADTDDDEHLRDITEASPSDRLLAGGSPKRSININYQRGTNHLEAAQKMLGQNCYFPLEGIRGKAHENHIGPDPARRPKAERGRQNTLDKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNH6</accession>
    <name>A0A3B5LNH6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Triokinase/FMN cyclase</fullName>
            <ecNumber evidence="5">2.7.1.28</ecNumber>
            <ecNumber evidence="4">2.7.1.29</ecNumber>
            <ecNumber evidence="6">4.6.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)</fullName>
        </alternativeName>
    </protein>
    <organism evidence="31 32">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="31" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. Represses IFIH1-mediated cellular antiviral response.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>D-glyceraldehyde + ATP = D-glyceraldehyde 3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13941"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.28"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>FAD = riboflavin cyclic-4',5'-phosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13729"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:76202"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.6.1.15"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>dihydroxyacetone + ATP = dihydroxyacetone phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15773"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16016"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="23">Homodimer. Interacts with IFIH1 (via the CARD domains), the interaction is inhibited by viral infection.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.28" evidence="5"/>
    <dbReference type="EC" id="2.7.1.29" evidence="4"/>
    <dbReference type="EC" id="4.6.1.15" evidence="6"/>
    <dbReference type="RefSeq" id="XP_027894049.1">
        <property type="nucleotide sequence ID" value="XM_028038248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNH6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009404"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009515.1">
        <property type="protein sequence ID" value="ENSXCOP00000009404.1"/>
        <property type="gene ID" value="ENSXCOG00000007137.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114157350"/>
    <dbReference type="KEGG" id="xco:114157350"/>
    <dbReference type="CTD" id="26007"/>
    <dbReference type="GeneTree" id="ENSGT00390000015415"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034012">
        <property type="term" value="F:FAD-AMP lyase (cyclizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004371">
        <property type="term" value="F:glycerone kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050354">
        <property type="term" value="F:triokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.340:FF:000001">
        <property type="entry name" value="Dihydroxyacetone kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10440:FF:000001">
        <property type="entry name" value="Dihydroxyacetone kinase, DhaK subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1180.20:FF:000003">
        <property type="entry name" value="triokinase/FMN cyclase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10440">
        <property type="entry name" value="Dihydroxyacetone kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1180.20">
        <property type="entry name" value="Dihydroxyacetone kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012734">
        <property type="entry name" value="DhaK_ATP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004006">
        <property type="entry name" value="DhaK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004007">
        <property type="entry name" value="DhaL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036117">
        <property type="entry name" value="DhaL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050861">
        <property type="entry name" value="Dihydroxyacetone_Kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02361">
        <property type="entry name" value="dak_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011049">
        <property type="entry name" value="PRK14479.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28629">
        <property type="entry name" value="TRIOKINASE/FMN CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28629:SF4">
        <property type="entry name" value="TRIOKINASE_FMN CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02733">
        <property type="entry name" value="Dak1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02734">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01120">
        <property type="entry name" value="Dak2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82549">
        <property type="entry name" value="DAK1/DegV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101473">
        <property type="entry name" value="DhaL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51481">
        <property type="entry name" value="DHAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51480">
        <property type="entry name" value="DHAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="20" id="KW-0170">Cobalt</keyword>
    <keyword evidence="14" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="18" id="KW-0456">Lyase</keyword>
    <keyword evidence="16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DhaK" evidence="30">
        <location>
            <begin position="8"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="DhaL" evidence="29">
        <location>
            <begin position="371"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-hemiaminal-histidine intermediate" evidence="27">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <begin position="55"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012578"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00018932"/>
        </source>
    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022827"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
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        </source>
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            <dbReference type="ARBA" id="ARBA00045490"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00046681"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR612734-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS51481"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="578" mass="59616" checksum="BE379F8D5740867B" modified="2018-12-05" version="1">MEKKLINSVDCCVDEALCGLVRASGGLALLKGHRVMLRSDLDSLKGKVGLLSGGGSGHEPAHGGYIGAGMLSAAVAGGVFASPPPASILAAILTLHQAGASGVLLIVKNYTGDRLNFGLAAEQARNQGVAVDMVIVAEDCAFDQPSKAGRRGLCGTVFIHKLAGALAEEGCLLDQIVSKMKEVLKGIGTLGVSLSPCSVPGCRPSFDLPPGAMELGLGIHGEPGIKRSKVASADEVVKTMIDHMTNPDSQSHLPLKSGDSVVLCVNNLGALSCLEMAVVTRAAITCLESRGVVVARVMSGPFMTSLEMAGVSLTLMKVNPETLRLFDSKTSAPAWPNLSTAAVSGRNCITEAPVMITRPQDDKHSKGPLSPVMHKVLNKICSTLLEKQEELNSLDRASGDGDCGNTHAQAARAVEEWLQDHVVPGCPGQLLSVLAGLVEEKMGGSSGALYSLFLTAAAGHVTEGKTNGAAWAAAVHAGTQAMRRYGGADPGDRTMLDALCPAADELMRLTTAPPAGQMVVLHAAVEKAAAGAEATRDLTARAGRASYIAAERVTLPDPGAVAVAAMLRAVVETLAEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQ56</accession>
    <name>A0A3B5LQ56_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIPF</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">cis-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000014243"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014420.1">
        <property type="protein sequence ID" value="ENSXCOP00000014243.1"/>
        <property type="gene ID" value="ENSXCOG00000010785.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010157"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039765">
        <property type="entry name" value="Yip5/YIPF1/YIPF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12822:SF3">
        <property type="entry name" value="PROTEIN YIPF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="7">
        <location>
            <begin position="104"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="272" mass="30364" checksum="427FCE63BAA7E2D6" modified="2018-12-05" version="1">MEEEDACAWLPEGSVTMQTFVQSEIRGIFRVCRKSSTLFVNVSTFCDVAIPNSVFYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCVCVCAVGNLSTFLSSLGDPSYHHRPQFHRVTIAAVVIFLYAWLVPIGLWAFLTWRQGTERQVGGYSFLETVCVYGYSLFIYIPTSILWIISYEWLRWTLIVVAMVISGSVLVLTFWPVVRDDTRVTAVATVVTIVVLHTLLAIGCKVDELGGGRGDGERVSLAAFPLTMKSHKSKLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQL3</accession>
    <name>A0A3B5LQL3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MIT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQL3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013337"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013502.1">
        <property type="protein sequence ID" value="ENSXCOP00000013337.1"/>
        <property type="gene ID" value="ENSXCOG00000010103.1"/>
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    <dbReference type="GeneTree" id="ENSGT00390000012235"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009686">
        <property type="entry name" value="Senescence/spartin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045036">
        <property type="entry name" value="Spartin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21068">
        <property type="entry name" value="SPARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21068:SF43">
        <property type="entry name" value="SPARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Senescence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIT" evidence="2">
        <location>
            <begin position="10"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="569"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="577"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="618" mass="67220" checksum="328CF3A5A93938B7" modified="2018-12-05" version="1">MAEPAELLVIKDQYELAQQSMNHGSAAQEADNKSEALVYYKKSRLHLRQGLEVPTGGERHQGVVWDKARQLQQKMKEMLGTIDIRLSDLEKSTPGDTRLLSNFSTPLYPDLVINGQPTNSSVLHLYPTIPDTTHSTSPAFISPIFPAVPNTHTLPSADLETVDMDSSTDLPPAYTPKPMDGHRSLVYDPAGGSRWSREPPDGKDLLSIPSGVQLFFVEPNGQVSSLSSPGYLRIITTDNHFDCPVLFCPCGFQVCDRLYPLSADTPVLLANSGIYMFPDYLAELPSSYVGLVLSSDLPTADCEMFQDVLTQLADLRIQGTEGAEGEVINLTDKIPLNPLHEQTDQMVPEGEKTTFLLPGWSEKMAQGILSGATKLSQSFAKGAEATGNVIQKGAAKIRDRITPEETPTDVSPQVTKGLNATKQATGGAVRVSKFLVNGISTIAEHVAEKVAPHVKKHGAKLVPESLKKSQDGQASNFDGAKFVATSSIRGLSTVWESLETGAKHVCKSVATETVTTVKYKYGNDAGQATDTALRSVGNIGVAAHNIDNLGLKALLKTTGKKTAKVMIKESDDEVKETEENKTQKDKHLTQAEKEVEMKEEATAKKKDVNKEQERRKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LR06</accession>
    <name>A0A3B5LR06_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Protocadherin-8</fullName>
        </recommendedName>
    </protein>
    <organism evidence="26 27">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Calcium-dependent cell-adhesion protein. May play a role in activity-induced synaptic reorganization underlying long term memory. Could be involved in CDH2 internalization through TAOK2/p38 MAPK pathway. In hippocampal neurons, may play a role in the down-regulation of dendritic spines, maybe through its action on CDH2 endocytosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">The N-terminal extracellular domain forms homophilic interactions; these interactions activate p38 MAPK via TAOK2 and trigger endocytosis. Interacts with CDH2; this interaction may lead to CDH2 cointernalization. Interacts with CDH11. Interacts with TAOK2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Postsynaptic cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Presynaptic cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027877448.1">
        <property type="nucleotide sequence ID" value="XM_028021647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LR06"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010219"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010342.1">
        <property type="protein sequence ID" value="ENSXCOP00000010219.1"/>
        <property type="gene ID" value="ENSXCOG00000007753.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114147100"/>
    <dbReference type="GeneTree" id="ENSGT00940000155219"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000120">
        <property type="entry name" value="Protocadherin 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000001">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000002">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000003">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000007">
        <property type="entry name" value="Protocadherin alpha 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000117">
        <property type="entry name" value="protocadherin-8 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF46">
        <property type="entry name" value="PROTOCADHERIN-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 21" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 24" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017336453" description="Protocadherin-8" evidence="24">
        <location>
            <begin position="22"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="698"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="25">
        <location>
            <begin position="35"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="25">
        <location>
            <begin position="136"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="25">
        <location>
            <begin position="245"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="25">
        <location>
            <begin position="367"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="25">
        <location>
            <begin position="464"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="25">
        <location>
            <begin position="579"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="729"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="861"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="927"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="731"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="22">
        <location>
            <begin position="763"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="939"/>
            <end position="949"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00056898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00064553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00067805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1022" mass="110290" checksum="DEED662BBFA0D700" modified="2018-12-05" version="1">MGGRGWNGLLVFTCASVLVLAAFTQGKTMKYQTFEEDAPGTVIGNLAKDVYSTSPFSGGSRNSFKMMKQFNSSFIRLRESDGQLTIGERVDRERICKHTLHCLIAFDVVSFSKEQFKLIHVEVEVRDINDNSPEFPRKESSLEISENTAVGTRIPLDFAVDEDVGVNYIQSYQISVNSHFSIDVLSRADGVKYAELVLMKELDRETQASYALELVAMDGGNPSRSGTTRINVKVKDYNDNSPVFDRSSFSVDLPEDAPVGSLLLDLNAEDPDEGLNGEVVYGFGNQVPPEIRQLFRVDRKTGRLTLESPVDFESKNTYEFDVQATDLGPNPSPAICKIVVQVQDVNDNAPEISITPMTSITAGIAYITEAAARESFVALVSTSDRDSGANGQVHCTLYGHDHFRLQQAYEDSFMIVSTSPLDREKIPEYNLTVVAEDLGSPPFRTISQYTIRLTDENDNAPVFSKPVYEVAVVENNAPGSYITTVVARDMDMGSNGKVTYKLADTYFMGSPISTFVSLDPASGSLYALRSFNYEVMKQLELRITASDGGSPPLSGSASVYVRIIDQNDNAPLILQPPLNNGSAEVLLSRDAPTGYIVTRVEARDADEGMNSELSYGLATGEPSVFSVNKVTGDIYLNQVLSHDVDETLSVTVTVSDNGRPALTSTATLHFLIIAGSPPSDRTVYQAGGGDEARAQWDLSVVIIVVLAGSCTLLLLAIILIATTCNRRRRDKSGEDSDSYGEKGTLERGRGHAGDNPLLPLHGAAGGGGRAGGEGGGAEVFDGHSFSSQPGAFTPAHPGGSDMCSASEDGSEVPCVYDSDNNSKVRGNKHEGYSTLPGYGNGKEAVRPITIWKGNSYTTISARDPAFSGKDSGKGDSDFNDSDSDVSGDTGLKKDGAMVPPMGGQNALWACTSECKVLGHSDRCWSPSATRANAAPSPAPTLSSFSSLPKTASLPRDPNRRDNYYQAHIPKTVGLQSVYEKVLHSEYDYVLVTPPRPARVQEISEVAIPVYTPTPTHCPNNDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRQ5</accession>
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        <name type="scientific">Xiphophorus couchianus</name>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="54" mass="6241" checksum="EBCC333802FA6DF4" modified="2018-12-05" version="1">MENYEEFCMRSLAMLQEGKLKKKTCEPLCSLKTCSVIRFHGRPILLPLVSKPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRQ9</accession>
    <name>A0A3B5LRQ9_9TELE</name>
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        <name type="scientific">Xiphophorus couchianus</name>
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            <taxon>Neoteleostei</taxon>
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            <begin position="9"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
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            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
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    <sequence length="118" mass="14069" checksum="688096234F843862" modified="2025-10-08" version="2">MLFKDVRLLLEATKDETVVKSQDLFTVVRYVSYNPVGRTMAWDWTTLNWDYLVNRYTINDRNLGRLPNRITTTYNTKMQLEITLSLNWKQALETVRNNIEWIQKNEAEIKAWLESNVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRR9</accession>
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            <fullName evidence="9">Uncharacterized protein</fullName>
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRS2</accession>
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        <property type="entry name" value="BRINP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057450">
        <property type="entry name" value="BRINP_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15564:SF4">
        <property type="entry name" value="BMP_RETINOIC ACID-INDUCIBLE NEURAL-SPECIFIC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15564">
        <property type="entry name" value="MACPF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19052">
        <property type="entry name" value="BRINP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25415">
        <property type="entry name" value="EGF_BRNP1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0338">Growth arrest</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="MACPF" evidence="7">
        <location>
            <begin position="96"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="14"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="785" mass="88521" checksum="DE6ABDCC92A0642C" modified="2018-12-05" version="1">FVWQCTTQQMISEQLASGGPSGKALHGSLSSPASSSSSSSTSSSPSSSSAWGFNTRQSGGQLDWLLSEKGPFHKCPEYTEFRERFQQGFSTRYKIYREFSHWKVNSLATEKRDFLKAPLPLAPEFLRNLRLLGRRPTLQQINENLIKKYGTHFLVSATLGGEESLTIFLDKQKLNKKSEGNSNSSVVSLELLHQLAASYFTDRESTLRRLHHLQIATSAIKVTETRTGPLGCSNYDSLDSVSSVLVHSPENKIHLKGLQDVLPEFLRGRFVEAALSYVACNSEGELLCRNNDCWCRCSPRFPECNCPFADIKVMEENLEKNKASWNNFNQEFMESDEFKAFLKRLPTDQFLNVSTVAKFWSGDLPLQKRYAQLEASTVLASGKAQKIVRKLFTLSKRCPKQARISLPRERPVGFWLGTVQSLLYCNEHGVLGTFSEAAKSCTCPVDQPTCQGAIPCVVGTSPNSCSQCATDNATRCGACHHGNFLHLGSCRPSVGASLDHYLNFDLDMPDAEVKYLLQRLDSRIEVHAIYISNDVRLGSWFNPAWRKRMLLTLKSNKNKSNLIHMLMGISFQICSTKNSTLEPVPVIYVNPFGGSHSESWFMPVNQPEFPDWERTRLDSTPNAQCYNWTLSLGNKWKSFFETVHIYLRSRIVTDDPTVNETLFYEPLDLDDQTSNLGYMKINTLKVFGYSMHFDPEGIKDLILQLDYPYTQGTQDAAFLMLLEMRDRINRLSPPAPQPLDLFSCLLRHRLKLSAGEAARIKDSLQIFNSKLPNAFPEPDLAQLCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSV4</accession>
    <name>A0A3B5LSV4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-methenyltetrahydrofolate + NADH</text>
            <dbReference type="Rhea" id="RHEA:22892"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.5.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSV4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012466"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012617.1">
        <property type="protein sequence ID" value="ENSXCOP00000012466.1"/>
        <property type="gene ID" value="ENSXCOG00000009423.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166353"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004487">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000001">
        <property type="entry name" value="Bifunctional protein FolD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000070">
        <property type="entry name" value="probable bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF7">
        <property type="entry name" value="BIFUNCTIONAL METHYLENETETRAHYDROFOLATE DEHYDROGENASE_CYCLOHYDROLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00766">
        <property type="entry name" value="THF_DHG_CYH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="13">
        <location>
            <begin position="39"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="14">
        <location>
            <begin position="173"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="333" mass="36263" checksum="34C4F97CCB7019FD" modified="2018-12-05" version="1">MYSKYVYILNNSFISVAVLKNKFFLFFSLNFSRHSAVVISGTELARQIHREIQRDVEELVAQGNMRPHLGVILVGDDPASHTYVKNKTRAATILGISSETVVRPSSVSQEELLGLIDKMNRDWRVSGLLVQLPLPEHINERAVCNAIAPEKDVDGFHIVNIGKLCLDQRSMVPATPAAVWEMIKRAGIETVGKNVLVAGRSKNVGMPIAMLLHTDRNHERPGGDATVTIAHRCTPRDQLKKLTMLADIIIAAAGVPALITADMVKKGAAVIDVGINRILDQNTGKVRLIGDVDFEGVKEKAGFITPVPGGVGPMTVAMLMKNTVTAARNALMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LT34</accession>
    <name>A0A3B5LT34_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Myomesin 2a</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000015168"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015358.1">
        <property type="protein sequence ID" value="ENSXCOP00000015168.1"/>
        <property type="gene ID" value="ENSXCOG00000011462.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157057"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd20951">
        <property type="entry name" value="IgI_titin_I1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000069">
        <property type="entry name" value="Alpha-protein kinase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000029">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000124">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000134">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000192">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000197">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000222">
        <property type="entry name" value="Myomesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000179">
        <property type="entry name" value="Myomesin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000670">
        <property type="entry name" value="Myomesin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050964">
        <property type="entry name" value="Striated_Muscle_Regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817:SF22">
        <property type="entry name" value="MYOMESIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13817">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="93"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="195"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="6">
        <location>
            <begin position="307"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="6">
        <location>
            <begin position="435"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="6">
        <location>
            <begin position="535"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="6">
        <location>
            <begin position="631"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="6">
        <location>
            <begin position="736"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="1053"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="1246"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1335"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1347"/>
            <end position="1371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1372"/>
            <end position="1393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1417" mass="155640" checksum="3A80F40752985610" modified="2018-12-05" version="1">KSSFQSAQQVQTQATEAMAEPAYTIPASRTTAETEEYQRVSSNVGKGLAVIQEELHRMRIATKAQVESIAIQKEVKDMMKKRVDLLEEIPKMPDFLVALRPHTVWEKTPVKLFCTVEGNPRPIVKWYKGGVPVDPLSAPGKYKIESKYGVHSLIISRCVVSDTAEYSAVATNQHGTATSKAMVTVKSKLLHDIHPSKLEVTLLERFPVSFGVEGSSIRLSCTMVVVPDLPNVPPLAQWYRDDTLLKPSNLVEMSVGGGAASLTLPQLAKDDEGLYTLRIFTKDGTAEHSAYLFVSDAPASVKGAPGAPMSVKAYDVNSDYVLVAWKPPNTVNEAPITGYFVDRSVTGTDTWVQCNPAPVKVCKYPVSGLDVGHSYYFRVRAVNSAGISRPSRKSGKVTAIDAAESQRLQVIKIEGKYDIVIKDDELEGYVTIPGEPTDVHASEIFKLYVVLSWKPPSPRGRAPLWYIIEKVPGTGAWQRINTAVKLHSPRYPVFDLQDGQKYQFRVFSVNMYGSSEASAPSEPIQKVDEDGVPSAPGQIVATRNTKSSVFVQWEAPKHLKSLLGYYIDGRVVGAKEWFPCNHKPFRHNRFVVHGLIPGESYVFRVQAVNIFGLSEESQESSPIAVEPALATPSAPYGITMLSCDGSSMTLAWKKPKHCGGSKVKAYYIDKRDADTLVWKEVNLAAVTERICTVDNLTEGTFYEFRIQAANLAGVGLPSAPSTPLKCEAWTMEQPGPAYDLSFREVRGQSLVILWKAPIYTGADAVTGYFVDMAKKGSSEFVTLNGDPVTPHGFLQVTGLEEGSSYVFRVRAVNSKGVGKTSELSEPVCAKALPGTTEIVAGVDEETGDVFLSFEACEICETSKFEWSKNYKPIGDCPRATVTAKGRTSRLTFTNPDKDDLGRYSVVVTDTDGVSSSYTLTEDALNTMLELSYAIRHPVVPLKHNLNYEILEKGHVRFWLQAVKLSPSVSYRFIVNDKAVTSGEGQKISHDVASGVIQMTVDHFTRANEGTYTVQINDGKAKTQSSLVLVGDAFKAALKEAEFQRKEHIRKQGPHFSEYLDFTVTEDCTVMLTCKVANVKKDTVFHWYKEDDEVVPETAPNVMSGSCALPLPLFSRKDQGVYKAVLSDDKGKDTSVFDISGKVFDDIINAIARNAGASASELVMQCTPEGIRLQCYMNYYTEEMKTVWKHKDSKIASSEKMRIGGTAEMAWMQICDPSDKEKGHYAIEISDGKWVFVYRGRVVGGLPDVVTIMEEKSLSLTCTVWGEPTPEVTWFKNEQEVASNEHTRVTFDGGKFASLVINKVTTDDSGKYSINVRNKYGGEFVEITVSVYRHGEPIPEPKLGQPRAAPPPAQPVPPKSPAPSKTPTPSKPQTPAQSVKSPTPAPAATPGTKSPTPPRSVKSPTPPRFMKSPTPPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LU60</accession>
    <name>A0A3B5LU60_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">TGF-beta family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LU60"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011354"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011485.1">
        <property type="protein sequence ID" value="ENSXCOP00000011354.1"/>
        <property type="gene ID" value="ENSXCOG00000008583.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159784"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000012">
        <property type="entry name" value="Growth/differentiation factor 9 (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF19">
        <property type="entry name" value="GROWTH_DIFFERENTIATION FACTOR 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0339">Growth factor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017247354" description="TGF-beta family profile domain-containing protein" evidence="10">
        <location>
            <begin position="33"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="11">
        <location>
            <begin position="306"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="287"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="288"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="430" mass="49172" checksum="53DD47B7339E9C10" modified="2018-12-05" version="1">MEKQMLLSAAVGCFRALLLLILVCCSPPLVSPSDALHNLSALTYPYSSILSPLLKALSEHGGSRWSLDVRKKVKPEHRYVKYLTEVYKKSTRMQRSVDGGEVYNTIRLIKPQDECPAQSNKESFTQDLSYSLEQVRRKEQLLKSSLLYSFNLNNLAAVSSVCYLSIKKHEQANQCQLCAGISHTLNLTDSTQKRRRRNWVEVDVTLFLQPVQKRSVDLLVNISCPEEQRAGSNGFRSPFGFTLRSPSLILYLNDTSKVVHQRSLVSSSADLRSPTALDTFQKQVFKSAQRHGQKRRWRRASPKSKRGDKSLDMQLPELKSSSEFPTTDCALYDFRVRFSQLKLDHWIVFPPKYNPRYCRGICPRTMGFIYGSPVHTMVQNIIYEKLDFSVPRPSCVPSQYSPLSVMIFEEDGSYAYKEFKDMVATRCTCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVJ1</accession>
    <name>A0A3B5LVJ1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Tnfrsf1a-associated via death domain</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVJ1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015167"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015357.1">
        <property type="protein sequence ID" value="ENSXCOP00000015167.1"/>
        <property type="gene ID" value="ENSXCOG00000011489.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002016"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002947">
        <property type="term" value="C:tumor necrosis factor receptor superfamily complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005068">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097191">
        <property type="term" value="P:extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.680">
        <property type="entry name" value="TRADD, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035712">
        <property type="entry name" value="TRADD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009095">
        <property type="entry name" value="TRADD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036729">
        <property type="entry name" value="TRADD_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14913">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR TYPE 1-ASSOCIATED DEATH DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14913:SF0">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR TYPE 1-ASSOCIATED DEATH DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09034">
        <property type="entry name" value="TRADD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55044">
        <property type="entry name" value="TRADD, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRADD N-terminal" evidence="7">
        <location>
            <begin position="68"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="247" mass="27463" checksum="AFEF9D30637E5E72" modified="2018-12-05" version="1">MLFYFSLCDNVGNTNTKMADKTADGGAWTGCAVMFLQSLFPGVDLLALYKGEQGKFIIFKVLKLTLRDSAGGLGGYDILKVHDAEPFLGVEVKFVDVAACQQFLESYSSGAVLQSLNQHACRLLGLPQDFTVRTQLKAGPQNLDLCLDQLAVCLQHIHLSQPERLRDEEIDHLEQQLQSQALGPAPQTLTVTQEESPVPSNCFKFQNRVFVQEAEGDNRTAALQPRITSFQRQSPFYTAGHQQIMFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWD9</accession>
    <name>A0A3B5LWD9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="40">ATP-dependent DNA/RNA helicase DHX36</fullName>
            <ecNumber evidence="10">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="42">DEAD/H box polypeptide 36</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="41">MLE-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="46" type="primary">DHX36</name>
    </gene>
    <organism evidence="46 47">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="46" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="46" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Chromosome</location>
            <location evidence="9">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
            <location evidence="8">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWD9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015442"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015634.1">
        <property type="protein sequence ID" value="ENSXCOP00000015442.1"/>
        <property type="gene ID" value="ENSXCOG00000011687.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156903"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051880">
        <property type="term" value="F:G-quadruplex DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002151">
        <property type="term" value="F:G-quadruplex RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17981">
        <property type="entry name" value="DEXHc_DHX36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000002">
        <property type="entry name" value="Putative ATP-dependent RNA helicase DHX36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000670">
        <property type="entry name" value="Putative ATP-dependent RNA helicase DHX36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000739">
        <property type="entry name" value="Putative ATP-dependent RNA helicase DHX36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059023">
        <property type="entry name" value="RNA_hel_CTD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF237">
        <property type="entry name" value="ATP-DEPENDENT DNA_RNA HELICASE DHX36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26026">
        <property type="entry name" value="RNA_hel_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="30" id="KW-0007">Acetylation</keyword>
    <keyword evidence="35" id="KW-0010">Activator</keyword>
    <keyword evidence="32" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="38" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="21" id="KW-0221">Differentiation</keyword>
    <keyword evidence="33" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="23" id="KW-0347">Helicase</keyword>
    <keyword evidence="22" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="27" id="KW-0391">Immunity</keyword>
    <keyword evidence="17" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="25" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="34" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="37" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="47" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0678">Repressor</keyword>
    <keyword evidence="28" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="29" id="KW-0779">Telomere</keyword>
    <keyword evidence="36" id="KW-0804">Transcription</keyword>
    <keyword evidence="31" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="26" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="44">
        <location>
            <begin position="224"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="45">
        <location>
            <begin position="474"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="43">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="43">
        <location>
            <begin position="105"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="43">
        <location>
            <begin position="988"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="43">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="43">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="43">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="43">
        <location>
            <begin position="44"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="43">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="43">
        <location>
            <begin position="151"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="ARBA" id="ARBA00069921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="ARBA" id="ARBA00076143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="ARBA" id="ARBA00083335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="43">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="44">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="45">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="46">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="47">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1021" mass="115294" checksum="D6508685DCE1650B" modified="2018-12-05" version="1">MSYGYGGGGGRRSRKGDRGFRDGSRERLDRGGGGGGGGRGFRGHGSDFDQDRGAGGGQRDRPPPHLRGRDIGLWYARYGAVRRKQADRRSRAMVHMDEAREEHITKLLNSVQRDPTRDSRDAPSSVSDAWQTAASSSGHCDSSTAAKDEPPDNWDADDEDDVEKEKTLSEHKDLEFLFQEVVRDASVDEDLKRDLQSKKTDRNYKEMLKFREKLPSYGKKEDLVRLINSNRVLVVSGETGCGKTTQVTQFILDDHIRRGRGSTCRVVCTQPRRISAISVAERVAAERAESVGNGNSCGYQIRLQSRLPRRQGSILYCTTGIILQWLRSDPLLSSVSHLVLDEIHERNLQSDVLLVIVKELLNLRDDLKVILMSATLNAEKFSKYFDKCPMIHIPGLAFPVEEYRPQNQDRRPAWKRRFWQGHQSRSEKEEKEEEYKESWPCYARTLQGRYSDGTIEAVEMLDTDEKIDLELILALIRHIVLNEGEGAILVFLPGWDNISSLNDLLTAQQMFRSDRFVIIPLHSLMPTVNQTQVFKRPPPGVRKIVIATNIAETSITIDDVVYVIDGGKIKETNFDTDNNISTMTAEWVSLANAKQRKGRAGRVQPGKCYHLYNGLRASLLEAYQLPEIMRTPLEELCLQIKILKLGSISRFLEKALDPPSEKAVNLAIKSLTDLNALDHAENLTALGFHLARLPVEPHIGKLILFGALLGCLDPVLTIAASLSFKDPFFIPLGKEKMADMRRRTLSRNSKSDHLTIVYAFKGWEEAKQRGSRYERDYCWDNFLSANTLQMLHNMKGQFAEHLMSAGFVSSRDPKDAKSNINSDNEKLIKAVIVAGLYPKVAMIRPSYSKKRPGVKVYTQTDGKVCIHPKSVNAEEREFNYTWLIYHLKMRTSSIFLYDCTEVSPFSLLFFGGDITIQKDEGQETVAVDRWIVFRSPARIAHLVKSLKRELDSLLQDKISNPAPVDWENRQSKDCAVITAIIDLITTQERPAGGSKDDGRTWASAPSGRHSYFDDDDDDDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LX11</accession>
    <name>A0A3B5LX11_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphatase and actin regulator</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="5">Binds PPP1CA and actin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the phosphatase and actin regulator family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LX11"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016670.1">
        <property type="protein sequence ID" value="ENSXCOP00000016463.1"/>
        <property type="gene ID" value="ENSXCOG00000012427.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157628"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004018">
        <property type="entry name" value="RPEL_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12751:SF5">
        <property type="entry name" value="PHOSPHATASE AND ACTIN REGULATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12751">
        <property type="entry name" value="PHOSPHATASE AND ACTIN REGULATOR PHACTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02755">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00707">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51073">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RPEL" evidence="4">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU301113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="135" mass="15551" checksum="DEB480FC50EDFEEB" modified="2018-12-05" version="1">MCGYIFLIYKPGQSSVPNFLPMPPKGTCSTIFFQRGKLSTLGKLFKPWKWRKKKTSDKFQDLSKVLERKISTRQTREELIRKGVLIPEQGSSHFVYIGTKSPRLKSQADVSVWCLLVLPGDTWVLRLHQNQNHAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXJ1</accession>
    <name>A0A3B5LXJ1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Centromere/kinetochore protein zw10 homolog</fullName>
        </recommendedName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="21">Interacts with NBAS and KNTC1/ROD; the interactions are mutually exclusive and indicative for its association in two different vesicle tethering complexes. Component of the RZZ complex composed of KNTC1/ROD, ZW10 and ZWILCH. Component of the NRZ complex composed of NBAS, ZW10 and RINT1/TIP20L; NRZ associates with SNAREs STX18, USE1L, BNIP1/SEC20L and SEC22B (the assembly has been described as syntaxin 18 complex). Interacts directly with RINT1/TIP20L bound to BNIP1/SEC20L. Interacts with C19orf25 and ZWINT. Interacts with ZFYVE1. Interacts with RAB18 and this interaction is enhanced in the presence of ZFYVE1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ZW10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027888301.1">
        <property type="nucleotide sequence ID" value="XM_028032500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXJ1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012539"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012690.1">
        <property type="protein sequence ID" value="ENSXCOP00000012539.1"/>
        <property type="gene ID" value="ENSXCOG00000009478.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114153745"/>
    <dbReference type="KEGG" id="xco:114153745"/>
    <dbReference type="CTD" id="9183"/>
    <dbReference type="GeneTree" id="ENSGT00390000016427"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990423">
        <property type="term" value="C:RZZ complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.150:FF:000001">
        <property type="entry name" value="centromere/kinetochore protein zw10 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046362">
        <property type="entry name" value="Zw10/DSL1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048343">
        <property type="entry name" value="ZW10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055148">
        <property type="entry name" value="ZW10_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048344">
        <property type="entry name" value="Zw10_middle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009361">
        <property type="entry name" value="Zw10_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12205">
        <property type="entry name" value="CENTROMERE/KINETOCHORE PROTEIN ZW10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12205:SF0">
        <property type="entry name" value="CENTROMERE_KINETOCHORE PROTEIN ZW10 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20666">
        <property type="entry name" value="ZW10_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22766">
        <property type="entry name" value="ZW10_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20665">
        <property type="entry name" value="Zw10_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06248">
        <property type="entry name" value="Zw10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0007">Acetylation</keyword>
    <keyword evidence="19" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="20" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Centromere/kinetochore protein zw10 N-terminal" evidence="23">
        <location>
            <begin position="27"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Centromere/kinetochore protein zw10 middle" evidence="24">
        <location>
            <begin position="177"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="Centromere/kinetochore protein zw10 C-terminal" evidence="25">
        <location>
            <begin position="462"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="ZW10 C-terminal helical" evidence="26">
        <location>
            <begin position="612"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00065852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00069312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF06248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF20665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF20666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF22766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="767" mass="87549" checksum="DF48F8DED7BC527A" modified="2018-12-05" version="1">MASFVTEVLASSGKLEKEDLASKISRMSRKVEDTKEEVCDMINKKYSEFLPNLQASEDLMQQVDMVSKEMETLKTCIEFEVQQNIHVAVNEYIKLKQQLEKNTMIIKMLEHLKEFHSAMEDFNKTLQEKKYVDAATHLEKAWKNVDSLKDWKTSQVPLLSSLSSELTVQRENLIYHLGDEWKRLIIWRVPSTKESAGPKTFHKVELVLSEAYSKDLEQKPTPLLCSVLQALAIQGDLQNKIKLFSQVLLKNMLKPLVMYASLELIVSEQHGEGTILGLECLEEENKERSTPSQVYKKLLLLLKTLHMHLLDVSIGDKKLSAILGELVWEETSKCIIHDCLVYSIPTNSSELERYNIVIKETEAFEETLKNMEYLQLNSTDLFKYARDVNCHFASKKCRDVIVAARKFMTSKMHNTVKISPESKLRLPKLPPPGSEVKVKQEVVKEEVSMENSKQLSACSLRLPVCRISESVQQLMELAVNTLCEAVGSSTQCALQLFFTVRNIFQLFYDVVPTYHKENLLKFPHLAAVQHNNCMYLAHHLLTLGHHFRAHLPQPLSEGVATFVDMVPGFRKLGAQCFLAQLNVQRAELLERLSTAHNFCNLDDEDNYTAASKAVRQVIHQLKQLGTVWQDVLPVSIYCKAMGNLLNTAITEIITKIMMLEDISSEDGEHLHTLCQTIIEEGPLVFIPLVEENKNKKYQEEVPLYVKKWSAFKELVIVLRANLQEIVDRWTDGKGPLAVEFSSSEVKSLVRALFQNTERRAVALAKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYI7</accession>
    <name>A0A3B5LYI7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Forkhead box A3</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027855303.1">
        <property type="nucleotide sequence ID" value="XM_027999502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYI7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012859"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013015.1">
        <property type="protein sequence ID" value="ENSXCOP00000012859.1"/>
        <property type="gene ID" value="ENSXCOG00000009723.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114133514"/>
    <dbReference type="KEGG" id="xco:114133514"/>
    <dbReference type="CTD" id="3171"/>
    <dbReference type="GeneTree" id="ENSGT00940000166203"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000042">
        <property type="entry name" value="hepatocyte nuclear factor 3-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013638">
        <property type="entry name" value="Fork-head_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018533">
        <property type="entry name" value="Forkhead_box_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050211">
        <property type="entry name" value="FOX_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829">
        <property type="entry name" value="FORKHEAD BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11829:SF201">
        <property type="entry name" value="HEPATOCYTE NUCLEAR FACTOR 3-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08430">
        <property type="entry name" value="Forkhead_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09354">
        <property type="entry name" value="HNF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fork-head" evidence="6">
        <location>
            <begin position="147"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="4">
        <location>
            <begin position="147"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="248"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="305"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="446" mass="48297" checksum="0E0CA19544C9D677" modified="2018-12-05" version="1">MLSSVKMEAHDLPEWNTFYSEASEMYSSPSTMNPGLGSMSSMSYINLNPATASPAGMNMAYPSSSLSSSSLASMGTGPSHMSLSPVASSSLTPLGSTAPASLGSLSHYQNMSQSMSQLGYSSAGSLSRAGPKEIPPKPYRRSLTHAKPPYSYISLITMAIQQSSSKMLTLNEIYQWIMDLFPYYRENQQRWQNSIRHSLSFNDCFVKVARSPDKPGKGSYWTLHPQSGNMFENGCYLRRQKRFKIEDKAAKKGAKNQEGASGKGDRMGEDRSPTGGSEGAESAHSDNSHPSSSDDQPHQRNTLVSLDCPPLSSSHLHSPPASSASSSMSLSGSSPTSNSLLHSQTLAGGSHLLPSAMQPHMDLQSDPLKSLDPHYNFNHPFSITNLMSNEQKMDLKSYQDQFMAYNSYASSSPVGVKQIYDSSSPAAMDSGAYYQTLCSRSVLNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZ05</accession>
    <name>A0A3B5LZ05_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD225/Dispanin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZ05"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017338.1">
        <property type="protein sequence ID" value="ENSXCOP00000017118.1"/>
        <property type="gene ID" value="ENSXCOG00000012905.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007593">
        <property type="entry name" value="CD225/Dispanin_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04505">
        <property type="entry name" value="CD225"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="119" mass="13687" checksum="82DABA9378E9C31C" modified="2018-12-05" version="1">MSSQNDPESLAAHRTFVCWTQRLMVMKIVALCSPCEPFIRRIFFCFQARDRKVVGDQDGARRHAYTARALNITATVLIPIGPDLIKLSSFNLEGKRNLFHNINLINNFNPFVDSGWVWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZ87</accession>
    <name>A0A3B5LZ87_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulator of G protein signaling 17</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZ87"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016472"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016679.1">
        <property type="protein sequence ID" value="ENSXCOP00000016472.1"/>
        <property type="gene ID" value="ENSXCOG00000012435.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155393"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF196">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="84"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50132"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="210" mass="24588" checksum="E9C0FEF60772BD08" modified="2018-12-05" version="1">MKPHSVSGVEMRKRQAAHIEAPPQVPGQPRPNTCCLCWCGCCKCLWNEDRIERSERQTCTKMDSIEAAGEQRPSLEELLSWSRSFEMMLRSLEGREVFRDFLRSEYSEDNLLFWLACEELKKETNPSVVDEKARIIYEDYVSILSPKEVSLDSRVREGINQSLTEPSNMMYEEAQLQIYTLMHRDSFPRFLNSSVYRDLLDSRRRTCLDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0H7</accession>
    <name>A0A3B5M0H7_9TELE</name>
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        <submittedName>
            <fullName evidence="4">Coiled-coil-helix-coiled-coil-helix domain containing 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CHCHD2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="entry name" value="CHCHD2/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2/NUR77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523:SF3">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06747">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHCH" evidence="3">
        <location>
            <begin position="104"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="153" mass="16094" checksum="0E2838AA75C65891" modified="2018-12-05" version="1">MPRGSRSRPARMSPPASRAPPSPPPMASAPSHAPVPMQAAPAAAPRQPGMFAQMASTAAGVAVGSAVGHTIGHAMTGAFSGGRSEAASPDPYQQQAGQQQQEVCSYELKQFLECAQNQSDLKLCEGFSEVLKQCRSHGVPLEQLKEKTERRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0S2</accession>
    <name>A0A3B5M0S2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Sodium-coupled neutral amino acid transporter 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Solute carrier family 38 member 7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Symporter that selectively cotransports sodium ions and amino acids, such as L-glutamine and L-asparagine from the lysosome into the cytoplasm and may participates in mTORC1 activation. The transport activity requires an acidic lysosomal lumen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-asparagine(in) + Na(+)(in) = L-asparagine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:71383"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-glutamine(in) + Na(+)(in) = L-glutamine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:68236"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0S2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015211"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015402.1">
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        <property type="gene ID" value="ENSXCOG00000011526.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015182">
        <property type="term" value="F:L-asparagine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015186">
        <property type="term" value="F:L-glutamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000038">
        <property type="entry name" value="Putative sodium-coupled neutral amino acid transporter 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF192">
        <property type="entry name" value="SODIUM-COUPLED NEUTRAL AMINO ACID TRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="10 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="11" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017479064" description="Sodium-coupled neutral amino acid transporter 7" evidence="20">
        <location>
            <begin position="18"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="283"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="21">
        <location>
            <begin position="9"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="373" mass="41116" checksum="84AD1186ECE56F6D" modified="2018-12-05" version="1">QFMLIFIISGLVILGYCSQVSNEGTYQEVVRATCGKVTGILCELAIAIYTFGTCIAFFIVIGDQLDRLVAAAAQETDGSPEGHWYTDRKFTIVVTAVLVILPLSIPKEISFQKYASALSVMGTWYVTVVVIVKYIFPDKDMNPDHAPVTSASWTAVFNAMPTICFGFQCHVSCVPVFNSMKTKAIKPWGFVVTCSMIICLFVYTGTGVCGFLTFGSSVNQDVLMSYPPDDIAVAIARAFIVLCVVTSYPILHFCGRAVVEGLWLRFQGEQVEVCVRRERRRRILQTLVWFVVTLVLALFIPDIGRVISLIGGLAACFIFVFPGLCLMQAKLSETDIRTKSWHLLVFLGVAMVTLGAFIFGLTTSNSIYQDVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3T7</accession>
    <name>A0A3B5M3T7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Protein smoothened</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor Fz/Smo family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027899507.1">
        <property type="nucleotide sequence ID" value="XM_028043706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3T7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018025"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018252.1">
        <property type="protein sequence ID" value="ENSXCOP00000018025.1"/>
        <property type="gene ID" value="ENSXCOG00000013579.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114160844"/>
    <dbReference type="KEGG" id="xco:114160844"/>
    <dbReference type="CTD" id="6608"/>
    <dbReference type="GeneTree" id="ENSGT00940000157206"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005113">
        <property type="term" value="F:patched binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:adenohypophysis development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035479">
        <property type="term" value="P:angioblast cell migration from lateral mesoderm to midline"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:anterior/posterior pattern specification"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:arterial endothelial cell fate commitment"/>
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        <property type="term" value="P:cardiac ventricle development"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0010002">
        <property type="term" value="P:cardioblast differentiation"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:cartilage development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:caudal fin morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:cerebellum morphogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071679">
        <property type="term" value="P:commissural neuron axon guidance"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0035912">
        <property type="term" value="P:dorsal aorta morphogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:embryonic camera-type eye development"/>
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    </dbReference>
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        <property type="term" value="P:embryonic digestive tract morphogenesis"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0048702">
        <property type="term" value="P:embryonic neurocranium morphogenesis"/>
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        <property type="term" value="P:embryonic viscerocranium morphogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060956">
        <property type="term" value="P:endocardial cell differentiation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:endocrine pancreas development"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0072175">
        <property type="term" value="P:epithelial tube formation"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0031017">
        <property type="term" value="P:exocrine pancreas development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021508">
        <property type="term" value="P:floor plate formation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021798">
        <property type="term" value="P:forebrain dorsal/ventral pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021986">
        <property type="term" value="P:habenula development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001947">
        <property type="term" value="P:heart looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042693">
        <property type="term" value="P:muscle cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030239">
        <property type="term" value="P:myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014033">
        <property type="term" value="P:neural crest cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001839">
        <property type="term" value="P:neural plate morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060896">
        <property type="term" value="P:neural plate pattern specification"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014003">
        <property type="term" value="P:oligodendrocyte development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032474">
        <property type="term" value="P:otolith morphogenesis"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0045880">
        <property type="term" value="P:positive regulation of smoothened signaling pathway"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
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    <dbReference type="GO" id="GO:0060041">
        <property type="term" value="P:retina development in camera-type eye"/>
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        <property type="term" value="P:retinal ganglion cell axon guidance"/>
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    <dbReference type="GO" id="GO:0048752">
        <property type="term" value="P:semicircular canal morphogenesis"/>
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    <dbReference type="GO" id="GO:0048741">
        <property type="term" value="P:skeletal muscle fiber development"/>
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    <dbReference type="GO" id="GO:0048745">
        <property type="term" value="P:smooth muscle tissue development"/>
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    <dbReference type="GO" id="GO:0021523">
        <property type="term" value="P:somatic motor neuron differentiation"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0061053">
        <property type="term" value="P:somite development"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0021520">
        <property type="term" value="P:spinal cord motor neuron cell fate specification"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0048795">
        <property type="term" value="P:swim bladder morphogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021537">
        <property type="term" value="P:telencephalon development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001570">
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="7tmF_SMO_homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07451">
        <property type="entry name" value="CRD_SMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000010">
        <property type="entry name" value="Smoothened, frizzled class receptor"/>
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    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000068">
        <property type="entry name" value="Smoothened, frizzled class receptor"/>
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        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
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    <dbReference type="InterPro" id="IPR000539">
        <property type="entry name" value="Frizzled/Smoothened_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035683">
        <property type="entry name" value="SMO_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041771">
        <property type="entry name" value="SMO_CRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF35">
        <property type="entry name" value="PROTEIN SMOOTHENED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01534">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00489">
        <property type="entry name" value="FRIZZLED"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM01330">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
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    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017381437" description="Protein smoothened" evidence="19">
        <location>
            <begin position="26"/>
            <end position="811"/>
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        <location>
            <begin position="205"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="238"/>
            <end position="255"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="287"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="331"/>
            <end position="351"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="371"/>
            <end position="392"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="424"/>
            <end position="447"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="494"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="domain" description="FZ" evidence="20">
        <location>
            <begin position="39"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="21">
        <location>
            <begin position="202"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    <evidence type="ECO:0000256" key="2">
        <source>
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    <evidence type="ECO:0000256" key="3">
        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
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    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018025.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="811" mass="91582" checksum="9B7DA6A0A50E256C" modified="2018-12-05" version="1">MSSQGRSPIVGMLCVWAAWLSGCRAVMAPNGTMFEDNCKKTSTCEVLKYNTCLGSPLPYTHTSLILAEDSSSQEEAFEKLTMWSGLRNAPRCWSVIQPLLCAVYMPKCENGRVELPSKSLCLATRRPCSIVDRERGWPNFLKCDKFPVGCSNEVQKLKFNTSGQCEAPLVKTDIQSSWYKDVEGCGIQCDNPLFTEEEHNDMHAYIAYFGTITLLCTFFTLATFLADWKNSNRYPAVILFYINACFFVGSIGWLAQFLDGARKEIVCKSDNTMRLGEPSSSETLSCVTIFIIVYYSLMSGVIWFVMLTYAWHTSFKALGTTHQPLSGRTSYFHMVTWSIPFVLTVAILAIAEVDGDSVSGICFVGYKNYHFRAGFVLAPIGVVLVVGGYFLIRGVMTLFSIKSNHPGLLSEKAASKINETMLRLGIFGFLAFGFVLITFGCHFYDFFNQAEWERSFREYVLCEANVTIASQTNKPIPECTIKNRPSLMVEKINLFSMFGTGIAMSTWVWTKATFLIWKRTWFKIIGRSDNEPKRIKKSKMIAKAFAMRKELHKDPEKELSFSMHTVSHDGPVAGINFDLNEPSNDVSSAWALHVPKMVARRGAILPQDISVTPTGTPVPPPEERNRLWMVEADISPEMIKRKKKKKKRRKEVRNPDVVVENQTRLQREFGRSSVPRLPKLPCHPSLVANLREQQRQQTLEQGILPGSLPDYEPCEERCSYLERRSSRNGYLMTRVDCPENLGLRPSCHPLTSNWQPSGSSMYPREVERTDGLSERMAHVARVPAGRRAGYGPIHSRTNLMEAELMDADSDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M475</accession>
    <name>A0A3B5M475_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Claudin domain containing 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M475"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014960.1">
        <property type="protein sequence ID" value="ENSXCOP00000014774.1"/>
        <property type="gene ID" value="ENSXCOG00000011202.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002596"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042356">
        <property type="entry name" value="CLDN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14347">
        <property type="entry name" value="CLAUDIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14347:SF3">
        <property type="entry name" value="CLAUDIN DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13903">
        <property type="entry name" value="Claudin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="250" mass="28372" checksum="A6C9EFD36D9E5514" modified="2018-12-05" version="1">MVDNRYATALVIGSVLSLLATVYLSVAVGTQNWFQYNNTVQHPVQQCINTTVIKNEFLTGEFDEKTYSSHLFTWNGTLGLWWRCIQVPHCTHWYCCQQDPKTETLCVSFTLPQQFSTKVKQNTSEEDLLRTYLWRCQFLLPLVSLSLVFLSGLVGVCACLCRSFTPTLVVGLFHFIAGLCSLGTVCCFLSSVHLLNSEYKKPKNVGELVGWSLYLALISFPLQMMAAALFLWAARSHRKHYTRITAYRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4E7</accession>
    <name>A0A3B5M4E7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">STAGA complex 65 subunit gamma</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">SPTF-associated factor 65 gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Suppressor of Ty 7-like</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, SUPT7L, GCN5L2, TAF5L, TAF6L, TADA3L, TAD1L, TAF10, TAF12 and TAF9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4E7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014844"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015030.1">
        <property type="protein sequence ID" value="ENSXCOP00000014844.1"/>
        <property type="gene ID" value="ENSXCOG00000011249.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005572"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06847">
        <property type="entry name" value="HFD_SUPT7L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000034">
        <property type="entry name" value="STAGA complex 65 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006565">
        <property type="entry name" value="BTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039460">
        <property type="entry name" value="SUPT7L/Spt7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28598">
        <property type="entry name" value="STAGA COMPLEX 65 SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28598:SF1">
        <property type="entry name" value="STAGA COMPLEX 65 SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07524">
        <property type="entry name" value="Bromo_TP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00576">
        <property type="entry name" value="BTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Bromodomain associated" evidence="13">
        <location>
            <begin position="157"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="86"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="325"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="136"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="372"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00084075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="417" mass="45645" checksum="20C39524DA6BDBAC" modified="2018-12-05" version="1">MMRYWGEIPGPSGAPPSRSSFDLLQREFRSVEMQDPPLHQPSAQRPRPTTMLDIPSEPCSLTIHTVQLCQHARRLRALLAAAQGQAQGQSSASSEGGSRPEETEANLPLRPPTPPAVPDDLLPVDSKDPQQPFQLRHSDPESEFYKGKGEPVTELSWPSCRQLLYQSVATVLAHAGFESAQESVLETLTDLVHEHYLRLTQLLRVAVDREARLGATPFPDVVEQVFHEVGIGSMLALQRFWQVRIKDYHSYMLQVTKELSEEYERLVNPEKAMEDSKPLKIKDEPLSDIPFPVNEELEADLASGDQTLPMGVLGAHAERLAAGGGAANNSPLWPQVKMEPQDGDEGQGAGHHHHGVLSGDVFEEGDPMSTMSESGGAMAPSPGGAASEGSYASHSPDSLMGTSPVFNQRPRKRIKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M688</accession>
    <name>A0A3B5M688_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ring-box 1, E3 ubiquitin protein ligase</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M688"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018925"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019162.1">
        <property type="protein sequence ID" value="ENSXCOP00000018925.1"/>
        <property type="gene ID" value="ENSXCOG00000014225.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155618"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031461">
        <property type="term" value="C:cullin-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003222">
        <property type="term" value="P:ventricular trabecula myocardium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000978">
        <property type="entry name" value="Ring-box 1, E3 ubiquitin protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051031">
        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger RING-H2-type" evidence="10">
        <location>
            <begin position="40"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="149" mass="16593" checksum="FC2581DCF1670914" modified="2018-12-05" version="1">MAAAMDVDTPSGTNSGASKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNVTYRLWPGLWRPPPSDQAACAHTRTHTHTHTHTGTKITQKVANAAKCKTLCHKLAFRLEQTFLCLIRLRRRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6Y0</accession>
    <name>A0A3B5M6Y0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein 243, mitochondrial a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027899946.1">
        <property type="nucleotide sequence ID" value="XM_028044145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6Y0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015762.1">
        <property type="protein sequence ID" value="ENSXCOP00000015569.1"/>
        <property type="gene ID" value="ENSXCOG00000011770.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114161085"/>
    <dbReference type="KEGG" id="xco:114161085"/>
    <dbReference type="CTD" id="405855"/>
    <dbReference type="GeneTree" id="ENSGT00390000010221"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022564">
        <property type="entry name" value="DUF2678"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28603">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28603:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10856">
        <property type="entry name" value="DUF2678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13217" checksum="0516DF7E14B06156" modified="2018-12-05" version="1">MDEFSTRTYGTSGLDNRPLFGETSARDRIINMVIGGFTSVVVLVTVIGSFVFPSLPPQPLNIFFAVCILLACGSTIVLIFWYRQGDLEPKFRKLIYYMLATILLLCLCANLYFFDVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7H1</accession>
    <name>A0A3B5M7H1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Family with sequence similarity 83 member B</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM83 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7H1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015739"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015933.1">
        <property type="protein sequence ID" value="ENSXCOP00000015739.1"/>
        <property type="gene ID" value="ENSXCOG00000011894.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157889"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="protein FAM83H isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050944">
        <property type="entry name" value="FAM83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012461">
        <property type="entry name" value="SACK1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16181">
        <property type="entry name" value="PROTEIN FAM83A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16181:SF29">
        <property type="entry name" value="PROTEIN FAM83A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SACK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Scaffolding anchor of CK1" evidence="5">
        <location>
            <begin position="15"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="734"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="833"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="734"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="753"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="772"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="791"/>
            <end position="803"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="886" mass="101438" checksum="9C36AA24E178D1BD" modified="2018-12-05" version="1">MEFSLLSSLKGEVKHEDFVQPHYKESYRLAIDRLIKDGRDSYSEFLKEERIVSFLSEDEIVFLTSNVEQLPSQNQTEEIIDPTDNQSSSGTYWPTHSDVETPNLDLGWPHVQHEKHPTNINLLYHPPRLNNPTIKEVIRKHIQDARTVIAIVMDKFTDVDIFKEIVDASMRGVSVYVLLDDDNFKSFLKMADDHDIKLQQLRNMRVRTVKGPDYLCQSGAKFHGAMEQRFLLIDCHTAIYGSYSFSWSFEKIHLSMVQVITGRLVKSYDEEFRTLYARSMVRAELGTPLEGTNNISFLGQHMLSRHHSAPKIGRNDQMRHSLDVVNWKTCEMKSGTRDRLIKEDGKFLSPFKKTPQLELETLDEMNLCKRHSYAWETQDGCIPQNMRPRGSNWNIHGDSSILPHNYAMDNYLQVQQIRRGQHMRQSYNGLDKQVLSMQQNLPTLEKTSKSFMRTWRIESYLKNPDAPSPDTFDYLDQFEPVDKANSFMQGHMRNSIALRCPIPQILEPSRLINPSSCLTGYSATPNAPLHHSSMQWDAAAAENRMNTNEIMLKRKSLQILDNFDSYSSGKNSHHSTYSSLSRSKHGQIITNPDMLRDSWQKRHSVADPRSSTEHPYETSGHMYGHFVRTEVNRSTAEIPVQNGGYKTSMNEDQRSLSHYDVKNIADKKGPNNRIWHKPPTRTVSAAALILSNKELANKSKSMNSQPMPKNSSKKIQSLLNIPERKEDFRTMETASLNSCESSDTLTADDDARSTQSGGKHQQSSTSSVKSSPESHLKSEKPQVKTEEQQDHQVNPPSKTTIQKKPSFLGRNTKPAAENRVYSRFEPFCSLEKKSSLLPGSKRAQSKSLPRGETAFEASHNQATRGHQENKLEKFFHRVGHLLNKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7M2</accession>
    <name>A0A3B5M7M2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Titin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7M2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019490"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019730.1">
        <property type="protein sequence ID" value="ENSXCOP00000019490.1"/>
        <property type="gene ID" value="ENSXCOG00000014642.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286978"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048738">
        <property type="term" value="P:cardiac muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000031">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000002">
        <property type="entry name" value="Titin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000011">
        <property type="entry name" value="Titin b"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000034">
        <property type="entry name" value="Titin isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000003">
        <property type="entry name" value="Titin isoform E"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14340">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED GLYCOPROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14340:SF13">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="7"/>
    </dbReference>
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        <property type="entry name" value="I-set"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="62"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="157"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="257"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="355"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="545"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="645"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="744"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="843"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="5">
        <location>
            <begin position="1038"/>
            <end position="1133"/>
        </location>
    </feature>
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        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1181" mass="129905" checksum="0085B8EDA2102ADF" modified="2018-12-05" version="1">MAPKDSEAWTVCASVKGLETVVSNLLKGEEYQFRVVAVNEKGKSDPRQLAQSVVAKDLIIEPSVRPKSSSYSVQVGNDLKIEVPVAGHPKPTISWTKDGVALKQTTRVNVSDSAHHTTLTIKDATREDGGMYTVTIANALCSKDATIEVITLDKPGPPTGPIKIDDVSAESVTLNWEPPSYTGGCPISNYVVEKRDTTTTNWVVVSATVARTTLKVANLKTGGEYQFRIFAENRYGKSYAIDSVPVVAQYPFQEPGPSGTPFVSSYTKDYMVVEWHKPPSDGGSAILGYHLERKEKNSILWTKINKMLIQDCKFKSTPLEEGIEYEYRVYAENIVGIGKCSKVSEVYVARDPCDPPGRPEAVIVTRHSVMLRWTPPQYDGGSIVTGYLMEKRDLPEGKWMKASFTNIIENEFTVTGLTEDSKYDFRVIARNAAGAVSKPSESTGSITAKDEVEPPKCETDPIYNQTIVLNAGETFSLEASVEGKPIPTAQWFKGSVEVENSARAEIKNTDFKALLVVKDSIRNIAGIGPCTKSCEPVAARDPCESPHNLKVTNITRNSVSLFWDPPEYDGGVKIVGYIVECKELPNGRWLKCNFTNLQETYFDVTGLTEDAQYDFHVIAKNSAEMLSAPSECTGPVTIKDDVDPPTIILEDKFRQLVVIKAGEIIRIDAEITGRPIPVVSWSKDGKDIEAKARCEITSTNFTTTLIVRDAIRRDSGQYVLTLHNVAGTRSVAINCKVLDRPGPASGPLAVSGLTAEKCTITWGPPQENGGAEIMHYIVEKRETSRLAWTLVYGDMKATTCKVTKLLKGNEYIFRVRGVNKYGDGEALESEAAKALDPFTVAAAPTNVQVTSVTSEAMTICWERPVSDGGSSINGYVIEKREKTGLRWCRVNKKPFIHFTVSGLREKAEYYFRVKAENLAGYSDLGKLQNIYIFKFTIVIQKKIFRSKIKRHCKTSGSTLKCEIPLTGRPLPKVTLSKDNVVLKSTMRFNSEVTPDSIKIILRESIAGDSGRYDITASNSSGTTKSFVNIVVLDRPSVPLGPVELFDVTEESASLRWLPPAYDGGSPITNYIIQKRETTTANWIDVSSAVARCTIKVMKLTTGLEYQFRIRVAAENAAGLSVFSKASDAVLAIDACGEGKVLVLSGPKLTSPISRTHISLWLDLPRMKHMNSGSWPRMQLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M843</accession>
    <name>A0A3B5M843_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bridge-like lipid transfer protein family member 1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="STRING" id="32473.ENSXCOP00000017471"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017693.1">
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        <property type="gene ID" value="ENSXCOG00000013178.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:presynapse"/>
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        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="BLTP1"/>
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        <property type="entry name" value="BLTP1_N"/>
    </dbReference>
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        <property type="entry name" value="BRIDGE-LIKE LIPID TRANSFER PROTEIN FAMILY MEMBER 1"/>
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        <property type="entry name" value="TRANSMEMBRANE PROTEIN KIAA1109"/>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="685"/>
            <end position="705"/>
        </location>
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            <begin position="4"/>
            <end position="682"/>
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            <begin position="163"/>
            <end position="196"/>
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            <begin position="163"/>
            <end position="174"/>
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    </feature>
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            <begin position="184"/>
            <end position="196"/>
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    </feature>
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        </source>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="746" mass="86047" checksum="338FD8C15B12050C" modified="2018-12-05" version="1">MDEQGSNNTSFLNYEDLETYLNRHNSKFIWLLVATILSCGWIIYLTYYNSRNIGLFLTLIINRLYKGGYIHIGSFSFSVLSGKVMFRDVYYINQDMSIRIQDGFLIFRWWKMYNPKQKQHDPKAETRLFVTVNGFEFHVYNRTDLYARLQETFGLEPTLLSARRDEERGPEPRNHAQNHALDSVNVQTESPEPSSSWRSLIPVIKLNISTGRLAFGNHHLPQTLCVNFEDAFLTYATKPPSSHLDQFMHIVKGSLENVRVMLVPSPRYLGLQNDEPPRLMGEGFVVMQSNDVDIYYYQDEPGLVPVEQDGSEEAETSSEDDKLQDLPPCWGLDIVCGKGTDFNYGPWADRQRDCLWKFFLPADYQAMKVSEVAQPGKARQIQAFELRMNIIADATIDLLFTKNRETNAIHVNVGAGSYLEVNIPMTVGENGYSPNIKGQLLHVDTTSSMQYRTLLEAEMLAFHVIASYPCVWNMPQSWQCEIEVYKATYHFIYAQKNFFTDLIKDWASDSAPDIYSFVPYSWKFKILFHQFEMIWAANQHNWIDCSTKQQENVYLAACGETLNIDFTLPFTEFVPATCHTRFSLRAEDTDMRLSLPECHPSRYPLLTLAKDYQSVKLPGDGAASRDGQGAPSSKTSKPRWRNITKAEAGWVDCWSVPNFLLIIDYTWHPIYPQKADEQLKQSCRFWFWFWFWFCCCLLTFLLLFLTVEMEMSPDSQILLYGPLLRALIAIKVCSWGHPNASASSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M892</accession>
    <name>A0A3B5M892_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M892"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020513"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020763.1">
        <property type="protein sequence ID" value="ENSXCOP00000020513.1"/>
        <property type="gene ID" value="ENSXCOG00000015369.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156496"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000001">
        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.10:FF:000002">
        <property type="entry name" value="Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316">
        <property type="entry name" value="MEMBRANE ASSOCIATED GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316:SF10">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE, WW AND PDZ DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <feature type="domain" description="WW" evidence="11">
        <location>
            <begin position="60"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="11">
        <location>
            <begin position="106"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="189" mass="22069" checksum="D65699ADD13B8171" modified="2018-12-05" version="1">MDRIDSTAPEEEEEEERPPMVNGVGEHKDKADWRKTVPSYNQSAGAMDMRVWNTLDESQEPLPKNWEMAYTETGMVYFIDHNTKTTTWLDPRLARKAKPPEKCIEGELPYGWEKIEDPQFGTYYVDHINQKTQFENPVMEAKRKMINCSLCVLNMRKIDFSLNPSCIYVLDSHFKVKKRSAFCFAFSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8F0</accession>
    <name>A0A3B5M8F0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Tudor domain containing 7 a</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M8F0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019765"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020011.1">
        <property type="protein sequence ID" value="ENSXCOP00000019765.1"/>
        <property type="gene ID" value="ENSXCOG00000014820.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00890000139482"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043186">
        <property type="term" value="C:P granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030719">
        <property type="term" value="P:P granule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09974">
        <property type="entry name" value="LOTUS_3_TDRD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.610">
        <property type="entry name" value="LOTUS domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041966">
        <property type="entry name" value="LOTUS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025605">
        <property type="entry name" value="OST-HTH/LOTUS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037978">
        <property type="entry name" value="TDRD7_LOTUS_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050621">
        <property type="entry name" value="Tudor_domain_containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948">
        <property type="entry name" value="TUDOR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948:SF14">
        <property type="entry name" value="TUDOR DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12872">
        <property type="entry name" value="OST-HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51644">
        <property type="entry name" value="HTH_OST"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0744">Spermatogenesis</keyword>
    <feature type="domain" description="HTH OST-type" evidence="9">
        <location>
            <begin position="13"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="HTH OST-type" evidence="9">
        <location>
            <begin position="406"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="8">
        <location>
            <begin position="564"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="257"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="305"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1166" mass="130060" checksum="5E70BE3E2484B1A4" modified="2018-12-05" version="1">MLVVVLLPLFMSDSESIKKMLMAVLQSSKTGVSVSNLQSEYSALCGEFIPLKKLGFSKLEDYLRSIPSVVRLENRMGELRCFATVLEETAHIAKLVARQKNSKKSGRSQFVNCKMRYKPFNPYMLNVTPRSSLRQPSAGRYPNWTGNRFGAHGGHTMERRMTSPSPASRENVVQILICRGVSPWQPLSQIITALKNSWDFLFLFFTSMLRQFTTDFTELRSLGFLSFQVEKPFTSNRSDRLIYPLLSLDPPINPVININRSSPGSHLDSTRVSSHPSTSHQLSPSSKTGDSPRSPLAEPTFIFPPQSSTTASSKALPSVTLRSPGPNPILTPRLPRRGSHTMSFDGDSKIRVSPNQSTPPLAPTWTALASTHTSPDRDVLPLLKATFSSPSSSASTPPSTPCPAVVSDDMREKIKELLSKSSQGLWADALPKCFKDVYKMPFPEEILENLSSLCDICTVEYPFPHNKKRAILYYSCQVNVDLTNNQRTLISPLPSGLEALGPVVPPPLTQPPEDCPSVLVTEAKSTNALTIRYVGNNYSDAQEAMEDIMQIFYSRNSTKRPVSNPAVGQLVAVRGEDGDEVARAQVTEILDSNKVKVFYLDYGFSLETSQNSLLELHQDFLSLPFQAINVRLAGLEVFSSHPQVLSTLDKLAVGKILRMETLQPCQHSDVPTALLFDTSLEDDVNINSMCLKALQDSTMNNPLSVNVVLKDVFVTSVREDGTIFCQLPCRGKVRLRRFLENVQAFFTSQVTSESLVSRPFGGKFCLARCEDKWARAEITYMHDNRVMEILFTDLGIPATVTFTDVREIPRHFLKDFIIIPPQAVKCRLADLAVPEEGWSQEPFLLLNETLLSAKELKMKVVLKEYKGDRVVFMYLFDGGDGQKLDESINHHLAHSEVWQKVVSRNTNTVTSTGSNIVDTDLTSLVEKWTLGNPVPSPRIKTSSVPRAKDDAAVKTGTQPLPLPPPLDLPQSGQNMDVFVPMACHPGYFVVQPWQDLHKLEVLRGEMVLYYNQCGNTSTTMDIQKGRVYAAKFDKNWHRVQVKGVLSNGLVSVYDLDYGKHELVPRNLIQPLIEEFRQLPFQAIAAQLAGVAQRQWSEETSSLFRNHVERRALVAQVESVREASELKGELSARRLTVYLVDTSSEEKDVWIHSIMADIENELSSAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8Q0</accession>
    <name>A0A3B5M8Q0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M8Q0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016134"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016339.1">
        <property type="protein sequence ID" value="ENSXCOP00000016134.1"/>
        <property type="gene ID" value="ENSXCOG00000012193.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165311"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000358">
        <property type="entry name" value="Tau tubulin kinase 2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="147"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="347"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="456"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="573"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="663"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="785"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="151"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="250"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="364"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="484"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="509"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="671"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="706"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="760"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="797"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="825"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="864"/>
            <end position="879"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="893" mass="97367" checksum="420C9A9E853E851F" modified="2018-12-05" version="1">MQCLVPGLQDNGNMNGTSEQADILPPNCMVKDRWKVLKKIGGGGFGEIYEALDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGEMAKNSKQRCEVQLQFPISLGINYAIFTEQVLFLDTVCSSIFYLDGSLGLFFCSRAIVTPVPGDPQRENTEDGLRDEHFSDQENAPPPPPSRPPAETATQQNVGDPGEAWEDTDFNRNRLRISLNKEDEAGRGACPVSPVRGGGPESPTGQGRSLRYRRVNSPESDRLSAAEGRVDGYGQRSRMDMLGSPSRHVYSSQPAQMLSVDPGCRGDRQASGRQEVSAASVDQEAHSNAFIRSVPLAEEEDFDSKEWVIIDKEAELRDFHPTTSGTTDEEPEELRPLEEQEERRRLRAGGNVAKLEASRRSGGSPAQSPCHSLPSGRPRRRESEPTGPQRPVRDPVIQSEERIWQHALGLQAKRADFLTVMLTGTLPQRRSFATPDEEVPEPLGPNDDDVTKSESNSEASQKSTERSQDAAPSTLMAEDQRGPREHASAADADPDLEDASKTLVLFSPGDTRKSPCGGEAVLEGELATPNALNSRNDRLLTGEGIQPELSETGRLSPALRSELRPSTPIAPGSPPFTKVERTFIHIAETSHLNVMSSNGHSAKESDREVFTVTRESAADVDRQEQEVALLLKETGEESVPDQTSTEAKQPKTENGPSAVFSLSMDSSSKVTSPVPDRKDPSQEAKKHLSSCEEAVKLHPAGSEPSVGSKPRIRSRIPILISEEDSGSEQSSSLSARARLQQRARQLDLARLLVQKQQGRWMRKRMLSGTSSSLSSGDDERWRASETLSTTGSEEDVRVSDESRRSSRLKPTEEQGSKDCRSKIPRPVTPIKRPAGGAAASAASPLSLPDAKNTKGVSSVING</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8X9</accession>
    <name>A0A3B5M8X9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Exosome complex component RRP41</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Exosome component 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ribosomal RNA-processing protein 41</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">EXOSC4</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Component of the RNA exosome core complex (Exo-9), composed of EXOSC1, EXOSC2, EXOSC3, EXOSC4, EXOSC5, EXOSC6, EXOSC7, EXOSC8 and EXOSC9; within the complex interacts with EXOSC2, EXOSC7 and EXOSC9. The catalytically inactive RNA exosome core complex (Exo-9) associates with the catalytic subunit EXOSC10/RRP6. Exo-9 may associate with DIS3 to form the nucleolar exosome complex, or DIS3L to form the cytoplasmic exosome complex. Exo-9 is formed by a hexameric base ring consisting of the heterodimers EXOSC4-EXOSC9, EXOSC5-EXOSC8 and EXOSC6-EXOSC7, and a cap ring consisting of EXOSC1, EXOSC2 and EXOSC3. The RNA exosome complex associates with cofactors C1D/RRP47, MPHOSPH6/MPP6 and MTREX/MTR4. Interacts with DDX60. Interacts with DIS3; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027876196.1">
        <property type="nucleotide sequence ID" value="XM_028020395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M8X9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019992"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020239.1">
        <property type="protein sequence ID" value="ENSXCOP00000019992.1"/>
        <property type="gene ID" value="ENSXCOG00000015017.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114146402"/>
    <dbReference type="GeneTree" id="ENSGT00940000153348"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11370">
        <property type="entry name" value="RNase_PH_RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000010">
        <property type="entry name" value="Exosome complex component RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF0">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0271">Exosome</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="15">
        <location>
            <begin position="21"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="16">
        <location>
            <begin position="155"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="245" mass="26797" checksum="F3A5BA0D08986601" modified="2018-12-05" version="1">MAGLELLSDQGYRLDGRKSTELRKVQARMGVFAQADGSAYLEQGNTKALAVVYGPHEMRGSRSRALHDRAVINCQYSMATFSTAERKRRPHGDRKSTEMSLHLKQTFEAAVMTQLFPRSQIDIYVKILQSDGGNYSVCVNAATLAVIDAGIPMRDYVCACTVGFVDETPLADLCYAEESGGVSSLALALLPRGGQIALVQMDARLHQDHLGTLIEAAMTACKCVSKVLDEVVRQHLQETSVLSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9D6</accession>
    <name>A0A3B5M9D6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 3 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9D6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016580.1">
        <property type="protein sequence ID" value="ENSXCOP00000016374.1"/>
        <property type="gene ID" value="ENSXCOG00000011733.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244874"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15283">
        <property type="entry name" value="7tmC_V2R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004073">
        <property type="entry name" value="GPCR_3_vmron_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF538">
        <property type="entry name" value="OLFACTORY RECEPTOR C FAMILY, H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01535">
        <property type="entry name" value="VOMERONASL2R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017233476" description="G-protein coupled receptors family 3 profile domain-containing protein" evidence="13">
        <location>
            <begin position="21"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="571"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="608"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="644"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="686"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="719"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="764"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="796"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="14">
        <location>
            <begin position="571"/>
            <end position="834"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="884" mass="97755" checksum="1EAFDD27960B3438" modified="2018-12-05" version="1">MGDWICVISLLIVILRFFKAENDSSCEMYGSPEVSLLSKEGDFIIGGAFSIYSQTSVINLREFRFVQTMMFAIEEINNSSELLPDVSVGYRIFDSCGSTLPSTRAAMGLMNGEENTWSKNCSSVHAVIGASESSSTIAMQQISGVFQIPMISHFATCACLSNRKEYPFFFRTIPSDYYQSRALAKLVKHFGWTWVGAVRSDNDYGNNGMATFIEAANQEGICVEYSEAISRTGSRENVERVVRVIRRGTASVLVAFLAQGEMDVLLDEVLTQNLTGLQWVGSESWITANYLAKKRYAGILTGSLGFAIRKAKISGLLDFLLKVNPGQDPQSNLLREFWETTFGCSFGSSTPGTKSCSGFERLQDANNAFTDVSELRISNCVYKAVYAVAHALHSLLKCGPSGDSVSHSCILKDNSEQRQVAEQLQKVNFTLQSGEQVYFDDNGDPAATYELVNWQRNHAGDVVFMTVGKYDASQPNWNQFTMNKVKITWAAESWEKPLSVCSENCLPGFRQAIMKGKPICCFSCVPCAGGEISNTNNSAECSQCPSEYWSNVNHSKCVPKVIEFLSYGETMGALLTAFSLCGASLTLVVLCVFFRFRHTPLVKASNSELSFLLLFSLTLCFLCSLAFIGQPTAWSCMLRHTACGVAFALCISCILAKTITVVMAFKAKRPANMFPQCSAPLQRTSVIGCTLIQVFICVIWLAFAPPFPHKNIVYANEKIILQCFLGSPLAFWVVMAYIGFLALLCFILAFLGRKLPDNFNEAKFITFSMLIFSAVWLTFIPAYVSSPGKFTVAVEIFAILASSFGLLFCIFAPKCYILLLKPERNTKKHLMGRNQKSMINLSNKLDCGNTYFTRSAGLTNSCNNIPSKKASLNMLMFLCHSLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9X4</accession>
    <name>A0A3B5M9X4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Exosome complex component MTR3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Exosome component 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">mRNA transport regulator 3 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Non-catalytic component of the RNA exosome complex which has 3'-&gt;5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the RNA exosome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027859744.1">
        <property type="nucleotide sequence ID" value="XM_028003943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9X4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018076"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018305.1">
        <property type="protein sequence ID" value="ENSXCOP00000018076.1"/>
        <property type="gene ID" value="ENSXCOG00000013623.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114136134"/>
    <dbReference type="KEGG" id="xco:114136134"/>
    <dbReference type="CTD" id="118460"/>
    <dbReference type="GeneTree" id="ENSGT00940000153348"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11371">
        <property type="entry name" value="RNase_PH_MTR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000035">
        <property type="entry name" value="Exosome complex component MTR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF2">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT MTR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="14">
        <location>
            <begin position="45"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="274" mass="29880" checksum="F95DCD707DC9949A" modified="2018-12-05" version="1">MPTDTKRVRGPETSQSPLLFWSRPAKVLPSHGPRADGRQRDQVDVRPVFVRCGLVSQAKGSAYIEAGSTKVLCCVYGPRETERKDETDMKSGRLTTDMRFAPFSCPERGSWIQGSQDKDFSLMLQESLQPAVCLHKYPRSQIEVNVMVLENSGSVLAHAVTCASMALADAGIEMYDLVLGCSLRQDGGSYVVDPTYPEENSWRSGSGEKQGGLTVAFLPSLNQVSGLQSDGEMAEETLTAGIRTCIEGCYKLYPVIQQALIKAVRRAALPPAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MA99</accession>
    <name>A0A3B5MA99_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000020480"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020730.1">
        <property type="protein sequence ID" value="ENSXCOP00000020480.1"/>
        <property type="gene ID" value="ENSXCOG00000015328.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154764"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000005">
        <property type="entry name" value="Photoperiod-independent early flowering 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031575">
        <property type="entry name" value="EP400_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46459:SF1">
        <property type="entry name" value="E1A-BINDING PROTEIN P400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46459">
        <property type="entry name" value="E1A-BINDING PROTEIN P400-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15790">
        <property type="entry name" value="EP400_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00573">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSA" evidence="14">
        <location>
            <begin position="784"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="1022"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="1711"/>
            <end position="1874"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="11">
        <location>
            <begin position="2162"/>
            <end position="2224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="534"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="694"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1402"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1525"/>
            <end position="1553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1918"/>
            <end position="1945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2497"/>
            <end position="2525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2730"/>
            <end position="2788"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="2321"/>
            <end position="2351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="28"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="551"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="694"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="715"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1409"/>
            <end position="1423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1527"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1922"/>
            <end position="1932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="2499"/>
            <end position="2525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="2760"/>
            <end position="2771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="2788" mass="305321" checksum="3080032BAF81F452" modified="2018-12-05" version="1">MHHGSGSQNMQRQLQKSKSVSGSEAEEQQQQPSMAATQQQATASHPQSPVTTFSSAASPSAPQSPNYQIIMSRSPVTGQNVNITLQNVGLAANQQITLTPLPIQNPASPGFQHSASQWRFEHAPSSYIQVTSPVPQPIQPQSPTQHSPVSLQGVTRPGAPPTALGVRGQSPTRFVEASMIVRQISLGSQAGGSHFVYQDGTALAQLAPPPGQVQLASTGAPGSVRERRLSQTHSQTGGTIHHLGSQSPVAAGTTLPTLGSPGHITTSSLPPQISNIIQGKLGRPIVFEKTAQGVVTGVGTPATATFSIPSSIPPSSSSLTSPPPGISSNPLTPTGMIAASIKKQAPKKLEEISPATPEIAQLRKQCLEHHTKKMESLKEVFKEYMVELFFLQHLQGNIMDYHAFKKKHCHPLNTYLRQNDLDLDDEDDEEEQSEVINDEVKVVTGKDGQAVTPVAIATQLPPNVSAAFSTQQQFQVHQGGASGSITNPGDMDAFKRQQAMVQTQAKRPRIDVGRHGLIFQHPGVASGVPLQQLMPTAQGMPPTPQAVQLAGQKQNQQQYDPSKGPPVQNAASLHTPPPQLPSRLPQGALPMASIPLALSQQAQLVENSTHPGVQVHVQAGVPVLAAMNPHTQLQSQLQQMQPGVHIQLQSQQQQSQTNLQPGQATVALARPGTDPNQPAQRIMTNSVSITSVVPAPSSAANSNPHTAGSLRPLGSNVNPGTPSKLTGTNGISSVKIGGFSQNTTAQAPQEGSQDRQVEQAKLESQVHQRISELRKEGQWSASRLPKLMEASRPKSHWDYLLEEMQWMAADFAQERRWKEAAAKKLVRTCARYHQEQKKNEERSQKQREVHLRHIASTIAREVEFFWSNIEQEESTIEEQEVMEGETDHKAELINLAKDEMSLDALKKQYAGAYADDFEWPQPSSCSDEDDVEETEMECVPSSPPEAVVIDSLLSMDQQRSTDKSMSNSNGKAGRDIAEVAAATELLLPKGSLRITTSTRSPAPVLLHGLLREYQQIGVDWLVNLHKKRLNGILADETGLGKTVQTVAYLAHLAGQEIWGPHLIVVRTCKLLSWELEFKRWCPGLKILLYLGNRRERRYKRMQLWEDSNSFHVCVTSYKLLMKDQRHFLKKRWRHLVLDEVQLIKNMTEKHWETIFALREQRILLIHTPLQNTLKEFWTMIHFLLPGITRPYSHFPIKADSDENQDYCHKLVIRLHRMIQPFILRRSKRDVEKQLPKKYEHILKCRLSNRQKTLYEDILTQPTQEALKSGHFVSVLQVLMQLQRVCNHPELVVPRESSSSYFSASLQYNVPSLVLGAIEKDSSQTANLSLFNLINENRLTRYQTEEAVPKLKVTQQLIEEIYTAPEPPPRPKPCPIKPMLFQPVQYGTKPEGRLAPIGNVAGQRPPTSSPPTGTLVSTVSQSGQARGKSPVTAPTAAPTSHGDVVKIAQLANMAGSQNRISQPETPVTLQFQGNKFTLSHSQLRQLTTGQPLQLQGTLNILQIVSAPGQPIIRPQGSMVMQTMPQTVPASSAAATPGTPHPGFSTAQQEASEEKTQQLKERLSRLFEANEQRCNRRVLYGSDLLQACTLSPEPAHTALTAGGWRWVGRESCSRAQRTCVGTTSVLQSALLTVENHHEAAGSLIKLTCVVPAAVAPPPHLYAANPSVPYRVQQKSFQQRLLEASEPHSADIHHLVSRHFLGHPDLQLMQMDSKLEALAILLQKLRSESRRVLIFTQMFKMLDILEAFLDYRQLNYVRIDESLTFEETQENMRTFNTNKQVFCSILTNCCCSEVGTIVDADTIIFYDTDLNPSMDARTQEWCEKVGRSKDIHIYLESGNSIEEKLLKIGTKELIREVVAQGTDYSLAFLTQTIQDLLEVEATSGVKVEEFVVLHQEPSASESISPKVARPYIQALHSINLDAQSEEGKPQEEELGGRSSTESEIQTKEEPGLLEELNAVMEQLTPIERYALHYLEYLHIGDDEAALKERLECAKRGWELQQLQKLKEEEEEKCQLMEGEEDLFTYTREDAYNMEYVFDSEDGHTEIMPVWTPPTPPQDDNDIYIDSVMMLMYDTTPMPESKLPPIYIRKEHKRLKMDPSARKKKKGHGETVIPPRSLFEKASMLKVRREGKDQKKNFSIKQPPFAKPLPSLAKPAMEAGQDNPEWLISEDWALLQQAVKQLLELPLNLTIVSPAHTPNWDLVSDVVNSCSRIYRSPKQCRNRYENVIIPREEGKLVYEPNPKKKSKSIYKSKNSRPLRTCQIYTQDDNSSQIQLYSSRFELMKIIASKRSPPIKPLGMNPFQKNPKHASVLAEGISYDKPLPPIQVASQRAERIAKEKKALAEQQKAQQLAQQQAGAPQTQAAAGQAQTPAVGQAQVPQAAGVTGATAVPNAAVLTVGGNVIVNTGAGIPQSPFQANKRLASPVIPGTLSPAGTAGAQVVHAQQRAVAATAASTEVVTIATGGVRAVTPGTASAVVSTTLSPVQSQTRPLELLKMQKHKGPGQQVSAVTQGAQAGVAQQPSQVQPAQAGQASPQLAAVAAARPGAVLAANTLQVTRLTRVPTQAQAGQTAQVTLTKPPVVSVPAVVSAVTTMSGLSVAINQAQKTVQQLIPMKKQQHPAAIQAAAAQQKAGQPQQGQAAVQQKAAQSAQQQQKVTYAATQQLQPGIKTQFFTTTSIAQTQKPAGAQQIQPQGQPQTVTLAQAATSGQVQMIPTGTPTAQVVQQKIIPQQVVTPATQIQPQNPHSPAHPPAAPSAAESPGQLPGQAQQPTKNQARQGGVRAKTPAKPSGGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAM6</accession>
    <name>A0A3B5MAM6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAM6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018326"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018558.1">
        <property type="protein sequence ID" value="ENSXCOP00000018326.1"/>
        <property type="gene ID" value="ENSXCOG00000013797.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154129"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22521">
        <property type="entry name" value="KH-I_PCBP1_2_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000002">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000005">
        <property type="entry name" value="poly(RC)-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="12"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="88"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="7">
        <location>
            <begin position="216"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="295" mass="30981" checksum="229F4C862C7C9EEB" modified="2018-12-05" version="1">MDSGVIEGGLNVTLTIRLLMHGKKGESVKKMREESGARINISEGNCPERIITLAGPTTAIFKAFSMIIEKLEEDISSSMTNSTATSKPPVTLRIVVPASQCGSLIGKGGCKIKEIRESTGAQVQVAGDMLPNSTERAITIAGTPQSIIECVKQICVVMLESPPKGVTIPYRPKPSGSPVIFAGGQLTKLHQLAMQQSPFPIAPGNQGFTGIDASAQTSSHEMTIPNDLIGCIIGRQGAKINEIRQMSGAQIKIANPVDGSTDRQVTITGSPASISLAEYLINARLSSEATGLAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBE3</accession>
    <name>A0A3B5MBE3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000021568"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021832.1">
        <property type="protein sequence ID" value="ENSXCOP00000021568.1"/>
        <property type="gene ID" value="ENSXCOG00000016140.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153769"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005614">
        <property type="term" value="C:interstitial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="CDD" id="cd01482">
        <property type="entry name" value="vWA_collagen_alphaI-XII-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000444">
        <property type="entry name" value="Collagen alpha-1(XIV) chain isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000008">
        <property type="entry name" value="Collagen type XII alpha 1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000234">
        <property type="entry name" value="Collagen, type XII, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000480">
        <property type="entry name" value="Collagen, type XII, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000001">
        <property type="entry name" value="Collagen, type XII, alpha 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048287">
        <property type="entry name" value="TSPN-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF15">
        <property type="entry name" value="COLLAGEN ALPHA-1(XIV) CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00210">
        <property type="entry name" value="TSPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="63"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="12">
        <location>
            <begin position="184"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="382"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="561"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="648"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="748"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="842"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="13">
        <location>
            <begin position="932"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="12">
        <location>
            <begin position="1049"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1018"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1463"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1574"/>
            <end position="1683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="143"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1021"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1492"/>
            <end position="1501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1503"/>
            <end position="1513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1585"/>
            <end position="1596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1657"/>
            <end position="1681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1752" mass="190521" checksum="108506AEC2A92D6A" modified="2018-12-05" version="1">MVAGRVSSFNPRSVVFVSLTSHPPCLLVVIRGPRGTGAWQPCFCQIASGQMRLLLLPYSSVPAPRRLRFKVHSSRKLLVSWREPRGEVDSYLFLYNSLPGGQQREIIVSKSDPKVLITDYNPSKDYMISVIAIRGREQSRPLRGRFKEDESELQRLTDSDGPPDDANEISGADQFVCHTAAIADIVILVDGSWSIGRINFKLVRTFLENLVNAFDIGIDKTRIGLAQYSGDPRIEWHLNTYSTKEAVIDAVKNLPYKGGNTLTGLALNFVSDYCFKPESGSRVGLPKIGILITDGKSQDDVVPPAESLRKAGVELFAIGVKNADENELRSIASEPHHTHVYNVADFNIMSSIVEGLTKTVCEQVELQDKDIKQKFTPEKMEMPLNLETSEVTAQSFRVSWSPPAGNVEMYRVVYSPAQGGEPQEAVVAGSETSVMLKHLSSLTEYQLAVFAVYTDEASEALRGSETTLALPSVASLQLFDATHSTMQARWSHVEGVSGYMLLYAPITDDGDLNNKVADGVTEVELVGLTPDTAYTVTVYAMYGEEASDPKTNQETTLPLSPPRNLQFSDITHNSAHISWDSPPDGVKGYRIMWVMTDGLVTKEVGPVNSYDLSELASLMEYSVAIFALYNEGQSEPLTDGFTTTPVPGPLNLRSSDVYTGSFKVSWDHSATDIALYRLSWAPFSGGDTKEVILSGSENRYTLDGLSPSTKYEVLLTAIFEDESESDTVSVIETTLAITTTIHTTTTGKCQRWKCVLFDETTQSLEASWELEDTDVESYRISYTDLSANQEEETISVGGGQKSTLLQSLLPDTQYKVTVTPVYLDGQDGISASALGSTLPLLSPENLRVSEESYNRFRVSWDLPQSPTEGYRIAYRPIHVQSPVLETTVDEDVNSILLLNLLSGTDYSVQVTAFYPGGPSEPQLINAKTLFLGVSGLSTYQVRSNSMCVQWQPLLHATSYRVSIQSVLNGQRQELNLGGSASRHCFYDLTPSSQYQISIYTQMQEMEGPSVSITDMTLSEPTPVPTEPPSTEPTPTIPPAKEVCKEAKADLAFLVDGSWSIGDDNFMKITRFLYSTMGSLDLIGPDGTQVAVAQFSDDARTEFLLSSHGNKEALLEAIQKIRYKGGNTKTGRAIKHVKESIFTPEAGARRGVPKVLVVLTDGRSQDDVNKVSKEMQMEGYIIFAIGFADADYGELVNIASKPSDRHVFFVDDLDAVKKIEEQLITSVCEAATATCPSVLMSGNTLAGFKMMEKFGLVEKEYSTLPGVSLEPGSFNSFPCYRLHRDALVLQPTKYLHPEGLPSDYTISMMLRLLPETPQEPFALWEILNKDNEPMVGLILDSSGKTLTFFNHDYRGDFQTVTFEGPEIKKVFHGSFHKLHVTISKTSVKVVLDCSVVGEKSIRAAGNITTDGVEVLGKMVRSRAKRDSSAPFQLQMFDIICSTSWASRDKCCELPALVRNGRISGGPGIRGARGNRGEPGVTGPQGPMGETGPSGPPGPPGPQGPSGLSIQGPPVSLRLSNRSLLSIMSHFCLFVLQGDVQSQAAVRAIARQVCEQIIQSHFSRYNSILNQIPQQFSGSVRTVTGPPGEPGRRGPPGPQGEQGTPGRPGFPGTNGQSGQPGERGLPGEKGERGSPGVGSQGPRGPPGAPGLPGEGRAGSQGPPGQPGSPGTAGRPGNPGATGPTGPPGYCDQNSCLGYNVGGKYYPFIPELPFGFLFQFNYGHHGYHPGNSEDVELRSLGVVRRFSQSVVTEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBI0</accession>
    <name>A0A3B5MBI0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ERO1-like protein alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Endoplasmic reticulum oxidoreductase alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Oxidoreductin-1-L-alpha</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EROs family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBI0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018626"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018860.1">
        <property type="protein sequence ID" value="ENSXCOP00000018626.1"/>
        <property type="gene ID" value="ENSXCOG00000014018.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007753"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016972">
        <property type="term" value="F:thiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007266">
        <property type="entry name" value="Ero1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037192">
        <property type="entry name" value="ERO1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613:SF1">
        <property type="entry name" value="ERO1-LIKE PROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613">
        <property type="entry name" value="ERO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04137">
        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110019">
        <property type="entry name" value="ERO1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="111" mass="12829" checksum="7A2FBC98A9C47878" modified="2018-12-05" version="1">ISLIWVHLIKIKFPQVTGELDDCTCDVETIDSFNNEQIFPKLQPLLESHYFRFYKVNLNKPCPFWTADSHCGLRDCAVKPCSPNEVPEGIRSVPHDKVREFKVNTLHTSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCJ5</accession>
    <name>A0A3B5MCJ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCJ5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021265"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021525.1">
        <property type="protein sequence ID" value="ENSXCOP00000021265.1"/>
        <property type="gene ID" value="ENSXCOG00000015916.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158594"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008332">
        <property type="term" value="F:low voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070509">
        <property type="term" value="P:calcium ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045956">
        <property type="term" value="P:positive regulation of calcium ion-dependent exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF209">
        <property type="entry name" value="VOLTAGE-DEPENDENT T-TYPE CALCIUM CHANNEL SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="6">
        <location>
            <begin position="99"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="386" mass="43681" checksum="9BF3B4E7C692365E" modified="2018-12-05" version="1">MKILYKLKITLKKKFDFASDALKLTSVSLRTSHPFPTLPQVITTTLDRLWSVAVCIKTSADVQFHQVFANIAAAASLGEQSGRGAGEGRPFEPINFICVFCQAFDAFIYIFFAMEMVIKMVALGIFGRRCYLGDTWNRLDFFIVMAGSELPVPVVRSSSRRVPYLEKKNLGMRILVNLLLDTLPMLGNVLLLCFFVFFIFGIIGVQLWAGLLRNRCYPEENFTLTTGIGLPRPYYMADEDDERPFICSLPVDNGIMSCSDVPARREGGRACCLDKEDVVYRQSIGLSPEPLTNNSVSVAGLCVNWNQYYTRCHTGSSNPHKGAINFDNIAYAWIVIFQVITLEGWVEIMYYVMDAHSFYNFIYFILLIIVSLDLLLFKSKMLNTLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCP2</accession>
    <name>A0A3B5MCP2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCP2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019041"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019278.1">
        <property type="protein sequence ID" value="ENSXCOP00000019041.1"/>
        <property type="gene ID" value="ENSXCOG00000014312.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005318"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039672">
        <property type="entry name" value="MFS_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11328">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11328:SF44">
        <property type="entry name" value="SODIUM-DEPENDENT LYSOPHOSPHATIDYLCHOLINE SYMPORTER 1-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13347">
        <property type="entry name" value="MFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="175" mass="19123" checksum="F7DE858DDB15ECF6" modified="2018-12-05" version="1">VPSSLPVFLTMCVLMGFSVATVFLLPWSMLPDVIDDFALRHPSCKDLEPLFFSGYAFCSKLAGGLSVGLSTMILQVVGYKAGACNHGDEVVTALVVLFSPAPVTLLLIGMGIFHTYPVNERNGLELKQQKYEQTKESSQNQNCQQTLSSLVLMTQNQTDFTTVIHHLAGYPPPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5ME11</accession>
    <name>A0A3B5ME11_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Titin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5ME11"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019491"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019731.1">
        <property type="protein sequence ID" value="ENSXCOP00000019491.1"/>
        <property type="gene ID" value="ENSXCOG00000014645.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286978"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031430">
        <property type="term" value="C:M band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008307">
        <property type="term" value="F:structural constituent of muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048738">
        <property type="term" value="P:cardiac muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd05748">
        <property type="entry name" value="Ig_Titin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000031">
        <property type="entry name" value="Myosin-binding protein C, slow type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000112">
        <property type="entry name" value="Titin a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000034">
        <property type="entry name" value="Titin isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000073">
        <property type="entry name" value="titin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14340">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED GLYCOPROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14340:SF13">
        <property type="entry name" value="TITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="138"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="4">
        <location>
            <begin position="331"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="428"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="597" mass="66218" checksum="90ACC5AFE3F016FF" modified="2018-12-05" version="1">MVVEWKPPTNNGGSPILGYHLERKEKNSLLWAKLNKLLIPDARFKTTELEEGIEYEFRVYAENIAGLSPASKVSESTVARDPCDPPGTPEAIEITRNHITLQWTRPQYDGAENAAGVGKPSTPTQYIKACDATYPPGPPNNPKVTDHSSTTVSLSWSRPIYDGGAHITGYVVEMKEALDDEWIVCTPHTGVQDTHYTVKKLKENAEYNFRICAMNCEGVGDHVDLPGSVIAAEKLQAPEIELDADLRKMVNVRATATLRLFVTIRGKPEPEVKWSKADGTLNDTNCVRNSYIISDLQEGVRYFFRVLAVNELGVGLSATTDQVKVSEAPLPPGKITVVDVTRHTVTLSWEKPDHDGGSKITSYIVEMQPKGDEKWTVCSDAKALEATIEGLITGEEYSFRVTAVNDKGRSDTKPLAMPVLVKDITMEPTINLLFHTYTVKAGDDLKIDVPFKGRPQPEVSWKKDGSALKQTTRVNVLNSKTSSKITIKDATKDDVGKYEITLTNSIGTKTAEISVIILDKPVSNLTENNEYQFRVCAVNKAGAGNYSEASDLYRAYDPIGMSEDFMLFLNVDLYNLKWVYMQLLKIFTLFGLPRSAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEC5</accession>
    <name>A0A3B5MEC5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">UTP4 small subunit processome component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">UTP4</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEC5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018014"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018241.1">
        <property type="protein sequence ID" value="ENSXCOP00000018014.1"/>
        <property type="gene ID" value="ENSXCOG00000013569.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153533"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046351">
        <property type="entry name" value="UTP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44163">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44163:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="686" mass="77271" checksum="E356908AD928C23A" modified="2018-12-05" version="1">MGEFKVHRVRFFDYMPSAIRSMAFNSHTERLAVARADGAVEIFNFADNYFQEKQVIAGRDGRSIEALCWVGCRLFSAGLDGEITEYDLENLRPRYSIEAYGGPIWTISGNSEGTLLAQAGCEDGTVKMFEVLEDRIQFQRNLSRQKRIISLSWHPSGAQIAAGMMDMIRIFDSETHATHRLLVDRGVGASKSREVVVWSVACLNDHTIISGDSAGKVQIWDGLTGTLIRTHLVTKWDVLVLAVSQWQDESSVIAGTSEGTIVQFQFISSTVGQQDKLWVRTRTFKNHSHDVRALVHTDSAVISGMDTQLVVRPLLDKVEKNTQESALRKIAFPHRNLVSCAKKAGLLLFQFPDHLELWRLGESDRNTPGESLPVKRKPEKLIQLKTKLQGEDHICCSALSPCGSWLSYATVAAVRLYRLQISSNNISIQKVSKLPRELKSVHQLCFSSDSCRLFASSSFSSVVVVALDQTDCKYLHTLKHKSSRQPIHILSPSEDGQWLATANTDCEIHVFNLRKMKLHCSVPAYSSCPTAVSIHPTTGNLVSVHADQQIFEFSLKQKEYSEWSRQLQRQGLHPLWLQRDTPVTSIAFNPNNPAHIMLHDVFMFCIIDQSLPLPEAKTKFYNQMILRSLPEPQRLRESHAFKTCKNFQHLLSVNLLEDLSLVVVERPLVDIVAQLPAPVRQKKFAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFN1</accession>
    <name>A0A3B5MFN1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase M14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MFN1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019931"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020178.1">
        <property type="protein sequence ID" value="ENSXCOP00000019931.1"/>
        <property type="gene ID" value="ENSXCOG00000014969.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156919"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11308">
        <property type="entry name" value="Peptidase_M14NE-CP-C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000020">
        <property type="entry name" value="Carboxypeptidase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1120:FF:000004">
        <property type="entry name" value="Carboxypeptidase E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1120">
        <property type="entry name" value="Carboxypeptidase-like, regulatory domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057246">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050753">
        <property type="entry name" value="Peptidase_M14_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532:SF73">
        <property type="entry name" value="CARBOXYPEPTIDASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11532">
        <property type="entry name" value="PROTEASE M14 CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13620">
        <property type="entry name" value="CarboxypepD_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M14" evidence="11">
        <location>
            <begin position="34"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="480" mass="54152" checksum="C653040DAC9AD8A3" modified="2018-12-05" version="1">MGVRGFVKDAVSGAALSNVSIVVAGIRHDLTTFRHHNYADMELFLRKYSSEFSSITHLYSIGRSVQDRELYVMIISDNPNVHEHGEPEFKYVGNMHGNEVVGRELLLNLIEYLCRNYGTDPEVTQLVNSTRIHIMPSMNPDGYEVAMEGKRNIYGGRNNSNDYDLNRNFPDQFVTITEPRQPETIAVMGWLKSTPFVLSANLHGGSLVVNYPFDDDKEGLSHYSRSPDDETFKQVAKAYSQENPLMYKGHPCNDLDPEDYFEDGITNGANWYTVPGGMQDWNYVNTNCFEVTIELGCVKYPLAKELPKYWEQNWRALLQFMHQVHTGIKGTVSDQDKVGIPNATISVGVIDHNITTGHTGDYWRLLVPGTYAVTASADGYKPLTTYATVSKDQAEVVDFRLTRLNSENTGHASTVPQLTRSPSDRKLSSLIKELSLDQGLEQLVKSTATDNSFRYRRPKDLEAFLRGLTLNFPQITSLHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MH73</accession>
    <name>A0A3B5MH73_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta/gamma crystallin 'Greek key' domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027879570.1">
        <property type="nucleotide sequence ID" value="XM_028023769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MH73"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023946.1">
        <property type="protein sequence ID" value="ENSXCOP00000023663.1"/>
        <property type="gene ID" value="ENSXCOG00000017673.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114148448"/>
    <dbReference type="GeneTree" id="ENSGT00940000163149"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000001">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF139">
        <property type="entry name" value="CRYSTALLIN, GAMMA M1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="15"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="54"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="143"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="189" mass="23257" checksum="C8829D66B1AAD1F6" modified="2018-12-05" version="1">MSTTETSQTDMNMRGKIIFYEERNFMGRHYECMSDCADMSSYMSRCHSCRVESGCFMVYDRPNYMGNQYFFRRGEYGDCMSMMGWRDWIRSCRMIPMHRGSYRMRIYERENFGGQMYEMMDDCDSIMDRYRMSDCMSCHVMDGHWLMYEQPHYRGRMMYMRPGEYRSFREMGYGGMRFMSMRRIMDSWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHY4</accession>
    <name>A0A3B5MHY4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta/gamma crystallin 'Greek key' domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHY4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024207.1">
        <property type="protein sequence ID" value="ENSXCOP00000023923.1"/>
        <property type="gene ID" value="ENSXCOG00000017861.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163149"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000001">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000003">
        <property type="entry name" value="Crystallin gamma S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF139">
        <property type="entry name" value="CRYSTALLIN, GAMMA M1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="3"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="42"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="5">
        <location>
            <begin position="131"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="177" mass="21787" checksum="19949F838C3C63D1" modified="2018-12-05" version="1">MHGYIIFYEDRNFQGRHYECMSDCADMSSYMSRCHSCRVESGCFMVYDRPNYMGNQYFMRRGEYGDYMSMMGWNDCIRSCRMIPMHRGSYRMRIYERENFGGQMHECMDDCDSFMDRYHMSDCMSCHVMDGHWLMYEHPHYRGRMMYMRPGEYRSFREMGYGGTRFMSMRRIMDSWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIJ4</accession>
    <name>A0A3B5MIJ4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Elongin B</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIJ4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024442.1">
        <property type="protein sequence ID" value="ENSXCOP00000024153.1"/>
        <property type="gene ID" value="ENSXCOG00000018032.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154348"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF255">
        <property type="entry name" value="ELONGIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="9">
        <location>
            <begin position="240"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="383"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="415" mass="46391" checksum="23E48DA29FE87CB1" modified="2018-12-05" version="1">MQTLSQMDFRQHVLSAGRAVLDMMEREWEPLSAGELELRLDQAVEEILESELVGRLEAQPPSPAVYLRLLRGNPSPGPRGSPTAAGLTGPTEKGYISKLLQGSTSGARLAGRARLSLSHTVLLSLSLLSERVTYRSVSRRFQLEKGNIHRIFFSFCERISTLTDEQIRWPLGEEAAEALFPFSGLVVDNQEDEGRSLPRVLGVLGHTQIPIRLPAGKHDLERLGPAEKRIKMAAHPDRWLHLELVCSRRGQFLHCRVSSGSDSDRGRILTDRLKQNPQLMPPGSCLVARTGYPLSARILTPYTGSCGPKQEVFNRTLAEHCRILDWAVGSLKARFKRLSYLDIGNYDRARTVVLTACVLHNVFLRMGQELRLDSEMENIRSAEEEEKDGEEDEEGVQRRDAAADLLLRNINSGIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJC1</accession>
    <name>A0A3B5MJC1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000023862"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024146.1">
        <property type="protein sequence ID" value="ENSXCOP00000023862.1"/>
        <property type="gene ID" value="ENSXCOG00000017826.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154352"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051983">
        <property type="term" value="P:regulation of chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007088">
        <property type="term" value="P:regulation of mitotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22673">
        <property type="entry name" value="FHA_Ki67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012568">
        <property type="entry name" value="KI67R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029334">
        <property type="entry name" value="PP1-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21603">
        <property type="entry name" value="ANTIGEN KI-67-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21603:SF17">
        <property type="entry name" value="PROLIFERATION MARKER PROTEIN KI-67"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15276">
        <property type="entry name" value="PP1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01295">
        <property type="entry name" value="K167R"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="FHA" evidence="8">
        <location>
            <begin position="26"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="195"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="717"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="947"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="227"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="344"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="389"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="947"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="987"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1053"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1138"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1170"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1326"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1367"/>
            <end position="1400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1430" mass="156110" checksum="20FAA1B4A9C3D2F9" modified="2018-12-05" version="1">MPLHGKIVVIKRRGGDGTEFPLTAPCLFGRKTECDIRIQLPQVSKEHCRIDLNENKEVNASANPTRVNGEALSQSERLKHGDVITIVDLTLTCKNIFLFSFLDPHLKDGANYNNIQRSLEKTLELESQKDEKNSPFNDLCQMIKKSLDVKTPRKSSASVVQTPSSKFCTPRLGPVVKKSEKGVVFIPKKEEDPKCKTPEIAKKQGKTFQVPSAEGPGPTVDKAGKSEGTSQQRRISTPQKFTASEVIEQTTASASKSPVRRRSKECTPAKSGVSMEQEGQAGKSPKLENPPKRSPKNSGSELGKDLPIPNLKRKRVSFGDHLSPELFDKRLPPDSPLRKGASPRRSLSLYKPKLSLLRRVSVIKEQRTPSPKFKKSASPKTPTGKASPKSRSTSPAEKSPRSNPAQTSPKSRSASPKATSPAQTSPKSRSASPKATSPAQKTPKSRSASPKATSPAQKTPKSRSASPKALTSTTPVKTPLNSGIQTPSVQGRFSVSRIRTPSPTTAVAQQMPLLAGTPKIPLRRKSMKNASRRTSGVARSAVKVFQRRSGISRASMKGVLYAETSAVTPARKLKDHMSTGHADSPATILVGRAHKRTIVHPTGAAPKVVMNPALFKKDMKMDDDLTGLSEMFKTPVNEKRRRSLVSESSAKKTPIATSSIVEPSVLNTPEEPDEMMVSPLSLASTVKSQRYNSEAVQRLLSGGEEASFVGDAPASEVCSESKHTDPKAGSVKTPKQKPELPICLTGIKRIMKTPRQKAEPIDDLRGKLLKTPKQKPVQQECLTGIKRIMKTPRQKAEPLEDIRGNLLVTPKQKTEQPECLTGVKRIFATPKQKVEPLEDLWGKLLKTPNTPKEKTAPVVDVVGMKRLPKTPKQKGEPVEDNFGIKRLMKSPRLRGNPPVEDFEGLQELMEEPVTYLTEQRPEKVIIGFRNIDTVILTLILFSDADKDKSVNSSDAERVKLPEEENANTEVAETSLTQKKSVRGKRAKPVEAKEAQKAEETSNRPVPPPVRGRRGNKMEATASPPAVEAQALTEISNEAVIPQISEKSNEKTTDPVSSVQVTTKPLRGKKAKVTSTEPERDECPAANAETPQVIPSVGKPRRGRKAKPDVEEPTEVAEDTCLPVETKPPTRAKRGRRALPKEDKQIEDDKVTSQEPSEHQEPPKKSRRTRKPEQEPLKAKEDTVVPENEPSSEQSSGAAKPRRGGRKAKADAVKPSVLMADATEKPKRGRKGEQIPQKTVPAKNPEHEEISAPSPQVHKPNRATPVKSKVSQTAPAKRGRRGAALLLVEPKQEPASEPVVPAKRGRRVAAKPKADDGASGEANPTESSKDDHQDPKMTKKAVTFKKYLESHEIPKATSVKAVRGRKTKVPDQADTRGKDGTKEASSTEEKTLSHDVVETTKRGRRGAKVAEVVAEDIGAQKKNRLGRSAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKX9</accession>
    <name>A0A3B5MKX9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Monocyte to macrophage differentiation associated</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MMD</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADIPOR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKX9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024407"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024701.1">
        <property type="protein sequence ID" value="ENSXCOP00000024407.1"/>
        <property type="gene ID" value="ENSXCOG00000018226.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157428"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140911">
        <property type="term" value="F:pore-forming activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005744">
        <property type="entry name" value="Hy-lIII"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01065">
        <property type="entry name" value="hlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="ADIPOR/PROGESTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF26">
        <property type="entry name" value="MONOCYTE TO MACROPHAGE DIFFERENTIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR604254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="229" mass="26104" checksum="2AD57B65746A931B" modified="2018-12-05" version="1">MNRRASANCRYQPTVYEHAANCYTHALLIVPAFVGMTLLHCLSDNNWERITAWVYGLGLCALFVVSTVFHIITWKKSHMRSVEQCFHMCDRVVIYFFIAASYTPWLNLRDLGPLAAHMRWFVWLMAAAGTIYVFNYHEKYKLVELAFYLTMGFFPASVVASMSNTDGLQELACGGLIYCLGVFFFKSDGVIPFAHAIWHVFVALAAAVHYYAIWKYLYNAASSKSLLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNI0</accession>
    <name>A0A3B5MNI0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Bromodomain PHD finger transcription factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNI0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021034"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021292.1">
        <property type="protein sequence ID" value="ENSXCOP00000021034.1"/>
        <property type="gene ID" value="ENSXCOG00000015736.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154830"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016589">
        <property type="term" value="C:NURF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15559">
        <property type="entry name" value="PHD1_BPTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000036">
        <property type="entry name" value="nucleosome-remodeling factor subunit BPTF isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038028">
        <property type="entry name" value="BPTF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018501">
        <property type="entry name" value="DDT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028941">
        <property type="entry name" value="WHIM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45975">
        <property type="entry name" value="NUCLEOSOME-REMODELING FACTOR SUBUNIT BPTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45975:SF2">
        <property type="entry name" value="NUCLEOSOME-REMODELING FACTOR SUBUNIT BPTF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02791">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15613">
        <property type="entry name" value="WSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00571">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50827">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DDT" evidence="9">
        <location>
            <begin position="98"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="248"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="419"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="468"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="492"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="685" mass="77798" checksum="901E95296536A916" modified="2018-12-05" version="1">IVATEQEAEDEFVEETPQSEEDEALNEESDCLEDDVLDEEQDDASFCTESSFRSQSTHASTPAKNKIRAPRPRTPVLEDKEIPPLELPETSEDLLVPSVELLNITSIYEVLRNFSSVLRLSPFRFEDFCAALVGQEQCTLIAETHISLLKAILREEDSSNTTFGPADLKDSVNSTLYFIDGMTWPEVLRAYCESDREFHRVLPYLETDEYPCGSLESKVKVLQFLVDQFLTTNIAREELMSDGSMQYDDHCRVCHRLGDLLCCETCSAVYHLECVKPPLEEVPEDEWQCEVCVAHKVPGVTDCVTEAQKNRPYIRQEPIGYDRHQRKYWFLNRRIVIEEDGECEKKKIWYYSSKAQLEELVEGLDKEYWEADLHATLEEMKEEMHAHMDITEDLTKRARGTNKAYLTAVNGTAYTCWPQNSGGGEHSTCEDAEGDEPSSPSRAESHERPCLADAPSDGDQLSAPGGLKKVEPPDQADRSSRSSFTSQDGTGKHKHGKTKHRKLNPPADKLLMPFPSPFQSSPAANTSRLTFLKRDLAVNLNNLFKLGQEGKYRVYQNQYSTNTLALNKHQHREDYDKKRHLSHKFSLTTASEFKWNGSIHGARSLTVATLRLTIVQLETNVPGPFMHPNWASHRYQYRCVARPGNLLWPWPSWSVQSNQSLCCLFGKTLSATQGKRGARLSLAFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MPL2</accession>
    <name>A0A3B5MPL2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Anion exchange protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Basolateral cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MPL2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023116"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023394.1">
        <property type="protein sequence ID" value="ENSXCOP00000023116.1"/>
        <property type="gene ID" value="ENSXCOG00000017269.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157488"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008509">
        <property type="term" value="F:monoatomic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008510">
        <property type="term" value="F:sodium:bicarbonate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000001">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.930.10:FF:000024">
        <property type="entry name" value="Anion exchange protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.930.10">
        <property type="entry name" value="Mannitol-specific EII, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013769">
        <property type="entry name" value="Band3_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003024">
        <property type="entry name" value="Na/HCO3_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016152">
        <property type="entry name" value="PTrfase/Anion_transptr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00834">
        <property type="entry name" value="ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF20">
        <property type="entry name" value="ELECTROGENIC SODIUM BICARBONATE COTRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07565">
        <property type="entry name" value="Band_3_cyto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01231">
        <property type="entry name" value="HCO3TRNSPORT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01232">
        <property type="entry name" value="NAHCO3TRSPRT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55804">
        <property type="entry name" value="Phoshotransferase/anion transport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="251"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="335"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="437"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="475"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="523"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="563"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="623"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="649"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="700"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="domain" description="Band 3 cytoplasmic" evidence="12">
        <location>
            <begin position="6"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="11">
        <location>
            <begin position="188"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="755"/>
            <end position="815"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="815" mass="91768" checksum="91677D197ED77E31" modified="2018-12-05" version="1">MNDISDTPSTDQLKNKFMKKIPRDAEASNVLIGEVDFLDKPFVSFVRLAQATTLGGLTEVPVPTRFLFILLGPQGKTKSYNEIGRAIATLMVDDLFSDVAYKARDRDDLIAGVDEFLDEVIVLPPGEWDPKIRIEPPKKVPSAEMRKSVLNLNELGQMNGSAGGAAGGEDDDLPVQHELGEELKFTGRFCGGLWLDIKRKIPFYCSDIYDGFHIQSISAVLFIYLGCITNAITFGGLLGDATDNYQGVMESFLGTALAGTVFCLFGGQPLIILSSTGPILIFEKLLYEFSKNNSIDYMELRLWIGLHSCLQCFLLVVSDASYIIKYMTRFTEEGFSSLISFIFISDAIKKMVGAFQYYPINRGFKPDYVTSYKCECVAPDQGETSNSLKKHPLFSPLLFKFLSFNLTDLDWSQLSKKECVKYGGVLVGSSCKYVPDLALMSFILFIGTYSMTVSLKKFKFSRYFPTKVRRLLHDFAIILAIMFFCGLDCLLELDTPKLHVPTQIKPTRSDRGWFVLPFGKNPWWWYLASFVPALLVTILIFMDQQISAVIVNRKENKLKKGCGYHLDLFWVGVLMAVCSFMGLPWYVAATVISIAHIDSLKMESECSAPGEQPQFLGVREQRLTGILVFVLTGLSVFLAPILQYIPMPVLYGVFLHMGVASLSGIQFWERMKLYLMPPKHQPDFSFLRHVPLRRVHLFTLVQIVCLAVLWILKSTFLAIIFPVMILGLMVVRKLLDLMFSQHDLAWLDDILPDKDKKKKEDDKKKKKEKKSVEPESDEESISPSSVKIHMEAVGPHTESDPTQVNPALKTKPLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQ19</accession>
    <name>A0A3B5MQ19_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RFX-type winged-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RFX family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQ19"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025878"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026189.1">
        <property type="protein sequence ID" value="ENSXCOP00000025878.1"/>
        <property type="gene ID" value="ENSXCOG00000019339.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244970"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000128">
        <property type="entry name" value="DNA-binding protein RFX5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003150">
        <property type="entry name" value="DNA-bd_RFX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039779">
        <property type="entry name" value="RFX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619:SF18">
        <property type="entry name" value="DNA-BINDING PROTEIN RFX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619">
        <property type="entry name" value="RFX TRANSCRIPTION FACTOR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18326">
        <property type="entry name" value="RFX5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02257">
        <property type="entry name" value="RFX_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51526">
        <property type="entry name" value="RFX_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RFX-type winged-helix" evidence="6">
        <location>
            <begin position="83"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="376"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="576"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="390"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="406"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="424"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="583"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="610"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="619" mass="66724" checksum="780287595540D24C" modified="2018-12-05" version="1">MSEDQQHQRAHASDRDKCGLEPGEGDTEPKLPRNDLMLFKQDVQRFSDNDKLFLYLQLPSGPSSGDKTGGDPSSFNTADQLHTCYWIRSHLEEHSDTCLPKQDVYETYRRYCENLQHRPLSAANFGKIIRDIFPNIKARRLGGRGQSKYPLNRCVNGIRRKTVLNMPLLPNLDLKNDPSELTELVQTYKQEVTEAACELICDWAQKILKRSFDTVVEIARYLIQEHIVNPRCNQAKLVTSAALAGLTSYAITNWFLFLVIWTNLCRSDFLPQSFSPITVPDKSFSVRSAVPPPVPVDAAVGVPSAHGGTAAITAPANSGGVKVITMTTLPQQQGRTLPVMILPQSCLSFEAEKVAPPPSLSAQQHTVAAPTSVVQKARGAAAKRPQEGEVSSIGVSAASSLSTSPVKRKRGRPRKPRPEDAVPSPSPQPPPPQPPIITPLTGGVIQKATSSLSSAAQTVVELVIQEPPGLVLGQLPAMSDPAHRLVDPRGVVVQCQPGGAADPDSQVRPVLLFHSPGNPSWERTPMVEVIQKAPRPNNNSGGGGGFGMAPFPPLPAATLHEEQGEVEITLTPVEVHVNPPPASTSSAAPASSLSISSTMVVKIEEEEVDNSPSPQKGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQC4</accession>
    <name>A0A3B5MQC4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GRB7/10/14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQC4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021689"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021953.1">
        <property type="protein sequence ID" value="ENSXCOP00000021689.1"/>
        <property type="gene ID" value="ENSXCOG00000016215.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155909"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005158">
        <property type="term" value="F:insulin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046627">
        <property type="term" value="P:negative regulation of insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01259">
        <property type="entry name" value="PH_APBB1IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000062">
        <property type="entry name" value="growth factor receptor-bound protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000056">
        <property type="entry name" value="growth factor receptor-bound protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015042">
        <property type="entry name" value="BPS-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039664">
        <property type="entry name" value="GRB/APBB1IP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039665">
        <property type="entry name" value="PH_APBB1IP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11243">
        <property type="entry name" value="GROWTH FACTOR RECEPTOR-BOUND PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11243:SF4">
        <property type="entry name" value="GROWTH FACTOR RECEPTOR-BOUND PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08947">
        <property type="entry name" value="BPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="Ras-associating" evidence="10">
        <location>
            <begin position="162"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="9">
        <location>
            <begin position="288"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="8">
        <location>
            <begin position="471"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="571" mass="65371" checksum="4902344049B19C70" modified="2018-12-05" version="1">MAVAGCPDYFLHHPNYQDNIDRSSNHRQADLIRPSFQPSTNQSSPNHQDDDVDLEALVNDMNSSLESLYSTCSGQQTEITPLLHNGQNPPSSSSANVDTPQTKLRRSQPMHILAVRKLQEEEQQFRTSSLPAIPNPFPELCSPASSPVLSPGSLPPGESSSEKHIVKIFSEDGMCKVVEIPADMTVRHLCQLLVYKSHCVDDNSWALVEHHPLLGLERCLEDHERVVQVQASMSSDSRFLFRKNYAKYEFFRNPLTFFPDHMVAWCQENDHKIPPSQLLQNFLSPSTCPEIQGYLYMKDVGRKSWKKVYMFLRRSGLYCSTRGTSKEPRYLQLLADLEDNSIFTVITGKKQHGAPTDYTFCFKLNKAIGETKELRMLCAEDEQSRTCWMTYGIILYQNYKQRKRSVSENSLVAMDFSGRIGRVIDNPIEAQSAALEEGHMWRKRNRVNALGSHSPLHQSSLNSVIHIAQLWFYGRITREESHRIIHQKRIPKIFFSHTDFSNPKAFVLTLCHHQKIKHFQILPCEEDGQVFFSLDDGETKFTDLIQLVEFYQLNRGVLPCKLKHPCTVVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQV7</accession>
    <name>A0A3B5MQV7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Caveolin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress. Mediates the recruitment of CAVIN2 and CAVIN3 proteins to the caveolae.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <location evidence="1">Sarcolemma</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Golgi apparatus membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <location evidence="10">Caveola</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the caveolin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027859593.1">
        <property type="nucleotide sequence ID" value="XM_028003792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQV7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026799"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027128.1">
        <property type="protein sequence ID" value="ENSXCOP00000026799.1"/>
        <property type="gene ID" value="ENSXCOG00000020016.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114136035"/>
    <dbReference type="KEGG" id="xco:114136035"/>
    <dbReference type="CTD" id="859"/>
    <dbReference type="GeneTree" id="ENSGT00950000183006"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070836">
        <property type="term" value="P:caveola assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042693">
        <property type="term" value="P:muscle cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007520">
        <property type="term" value="P:myoblast fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060035">
        <property type="term" value="P:notochord cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001612">
        <property type="entry name" value="Caveolin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018361">
        <property type="entry name" value="Caveolin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10844">
        <property type="entry name" value="CAVEOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10844:SF16">
        <property type="entry name" value="CAVEOLIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01146">
        <property type="entry name" value="Caveolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01210">
        <property type="entry name" value="CAVEOLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="76"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="152" mass="17490" checksum="191C3F3A769EB458" modified="2018-12-05" version="1">MADQYQYNTNEEKIVKDSHTKEIDLINRDPKQINEDVVKVEFEDVIAEPDGTHSLDGVWRLSYTTFTVSKYWCYRVLSAVFGIPVALLWGFLFACISFCHIWAVVPCIKSCLIESQCVSRIYSLCIQTFCDPFFEALGKIFSSVRVALRKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRM3</accession>
    <name>A0A3B5MRM3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Uridine phosphorylase</fullName>
            <ecNumber evidence="5">2.4.2.3</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the reversible phosphorylytic cleavage of uridine to uracil and ribose-1-phosphate which can then be utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. Shows broad substrate specificity and can also accept deoxyuridine and other analogous compounds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>uridine + phosphate = alpha-D-ribose 1-phosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:24388"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from uridine (phosphorylase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the PNP/UDP phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.3" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRM3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026433"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026753.1">
        <property type="protein sequence ID" value="ENSXCOP00000026433.1"/>
        <property type="gene ID" value="ENSXCOG00000019733.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157781"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00633"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004850">
        <property type="term" value="F:uridine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006218">
        <property type="term" value="P:uridine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17763">
        <property type="entry name" value="UP_hUPP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018016">
        <property type="entry name" value="Nucleoside_phosphorylase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010059">
        <property type="entry name" value="Uridine_phosphorylase_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01719">
        <property type="entry name" value="euk_UDPppase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43691">
        <property type="entry name" value="URIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43691:SF10">
        <property type="entry name" value="URIDINE PHOSPHORYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01232">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="6">
        <location>
            <begin position="49"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="133"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR610059-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="309" mass="33790" checksum="699BF2D5CD464D3A" modified="2018-12-05" version="1">MFNICPLSPSYSPVYVHNPHLDALKDDILYHFSLGTGSHNLPAMFGDVKFVCVGGSPWRMKSFTEFIAAELAMEDPKSGYPNICAGTDRYAMYKVGPVLSVSHGMGIPSIAIMLHELIKLLHHAHCTDVTIIRIGTSGGIGLEPGTVVVTSQSVDATFLPKFEQVILGKTVVRSTDLDQSLAQELLQCSKGLNQFETVIGNTMCTLDFYEGQARLDGAFCSYTEAEKQEYLLKAKEAGVCNIEMESSVFAAMCKLSGLQAAVVCVTLLDRLKGDQLSSSHEVLNNYQLRPQILVGSYIKKQLKAKAENR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MT44</accession>
    <name>A0A3B5MT44_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">cAMP responsive element modulator a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MT44"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026671"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026997.1">
        <property type="protein sequence ID" value="ENSXCOP00000026671.1"/>
        <property type="gene ID" value="ENSXCOG00000019924.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155408"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990589">
        <property type="term" value="C:ATF4-CREB1 transcription factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035497">
        <property type="term" value="F:cAMP response element binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14690">
        <property type="entry name" value="bZIP_CREB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000003">
        <property type="entry name" value="cAMP-responsive element modulator isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003102">
        <property type="entry name" value="CREB1-like_pKID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001630">
        <property type="entry name" value="Leuzip_CREB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45879">
        <property type="entry name" value="CYCLIC AMP RESPONSE ELEMENT-BINDING PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45879:SF1">
        <property type="entry name" value="CYCLIC AMP-RESPONSIVE ELEMENT-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02173">
        <property type="entry name" value="pKID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00041">
        <property type="entry name" value="LEUZIPPRCREB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50953">
        <property type="entry name" value="KID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="KID" evidence="9">
        <location>
            <begin position="13"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="196"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="51"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="214"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="27890" checksum="B0EC990F65FE10CB" modified="2018-12-05" version="1">KLWLQGNICIVETHYLMIVTVAELEDDGSVTDTQKRRELLSRRPSYRKILNELSSDSPTVPKIDEEKGEEDAAVSSSSTESVPNSIYQTSSGQYIAIAQGRAIPLTSPAVETLQGAQTVTVTGSPTPQTGAAVLQAADSSHQFFIQGGQVLIQAATGDMPAYQLRSPNSGLAQSIVMAASPSSMQSPSSQHAEEITRKREVRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDIYCHKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUD4</accession>
    <name>A0A3B5MUD4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Switch-associated protein 70</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
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        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Lamellipodium</location>
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        <subcellularLocation>
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        <subcellularLocation>
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        <property type="nucleotide sequence ID" value="XM_028027185.1"/>
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        <property type="protein sequence ID" value="ENSXCOP00000028024.1"/>
        <property type="gene ID" value="ENSXCOG00000020905.1"/>
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    <dbReference type="Pfam" id="PF25530">
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</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWV6</accession>
    <name>A0A3B5MWV6_9TELE</name>
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        <submittedName>
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        </submittedName>
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        <name type="scientific">Xiphophorus couchianus</name>
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        <source>
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    </evidence>
    <sequence length="358" mass="41159" checksum="D4AADAEB57B2174A" modified="2018-12-05" version="1">NSKSATDSPKHLSSREVREIAPKSEDTYEITIPFEENNFEQQGFFNQSDQNFDGKCPPTVSNSYMVINNLRTQLQISVEKNSWLQKRIEDLEEERDFLRCQLDRFIFSTKSQGPEQGQSQYSNEQQKADQQHFPQRQFVQRRPCPPTMVPSKNHISNPMSSQLSNMNALYNTTQSQALLQGHSQQASSTGSGSNSKHNSLSLLGESDEFLEEDGFIEEDEGDDVMSEAISTNRQQLKRRRVLRAPRERQRVKDAAGVLFRYKKILLTYQRLKNMTKAFQIHGVDRNTVASTTPIAELHLVAPEKVAEVGEFDSSKEKLLDYARRCYLALDEETLSRVQALKKNNLLLPISYRFRHEMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MYZ0</accession>
    <name>A0A3B5MYZ0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Si:ch73-22o12.1</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="CD80_C2-set"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="C2-set_2"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="16"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="134"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="348"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="376"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="501" mass="54298" checksum="C4EA0045BCED7915" modified="2018-12-05" version="1">SLLSALMGADLWTSGQRVKVEPELASYPGQTVNLRCAFPDPTGIQLTMVTWIYEPKDGERMNIAVFHPNFGPNYPESQMKGRVTFKPSPPELSSPSIEISNVRMTDEGRYVCEYATYPDGNEQGITQLVMLAKPLNSATAVTVNAGTQPVVVARCESKDGRPAAKIQWLTTAKGNWTETSQTGTDNTVTVTSEYRLAPTAMDNAKDLTCLVTHRTQLKDESLPLKLAIQYPPVVEIEGYDNNWYVGRTNVGLLLLGHYGEMPDTVQIKENELKVLKVDSSVNTTFVCEVKNSIGATREQVTINVRESLPPSSTESNAGVVAGAVIGSLLALLLVAALVTVLVTRSRRQQQGYRANGGSDMKTRIFGGGKKASKNGTSGGTGSGGVAGGNNNGPVYIDRSPNQGLGEKNNHHQPFTVGARPEVVTGTPTAQDILLSNELDEAERRKFDEMEEDERYDHFAGGGSILQLRPPPNPDDIIGDYMDDDMESQRDGSVISRTAVYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZB2</accession>
    <name>A0A3B5MZB2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transmembrane protein 47</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Transmembrane 4 superfamily member 10</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMEM47 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MZB2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027810.1">
        <property type="protein sequence ID" value="ENSXCOP00000027477.1"/>
        <property type="gene ID" value="ENSXCOG00000020515.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063484"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015664">
        <property type="entry name" value="P53_induced"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14399">
        <property type="entry name" value="P53-INDUCED PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14399:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="196" mass="21408" checksum="AA2D706D08A340AD" modified="2018-12-05" version="1">MSSAVTETEESAHSSPLTPLKLVGLVCVFLALCLDVGAVMSPAWVTADDQYYLSLWESCWKPGSFEKWECNSTLDKDWQVATLALLLGGGALVLMSFLVALVAVCIGTRRRFYALVAFMLFAAGHQVETVPTLKDDANVNIPIFHELNIFFFLLQQLFCSSAVELCFCGTSFSVTENTPCYNNGASAYVPLIAIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N164</accession>
    <name>A0A3B5N164_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Adrenomedullin 2b</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adrenomedullin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N164"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027220"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027553.1">
        <property type="protein sequence ID" value="ENSXCOP00000027220.1"/>
        <property type="gene ID" value="ENSXCOG00000020329.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154380"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010460">
        <property type="term" value="P:positive regulation of heart rate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003073">
        <property type="term" value="P:regulation of systemic arterial blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051665">
        <property type="entry name" value="Adrenomedullin-reg_peptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021116">
        <property type="entry name" value="Calcitonin/adrenomedullin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23414">
        <property type="entry name" value="ADRENOMEDULLIN, ADM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23414:SF2">
        <property type="entry name" value="PROTEIN ADM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00214">
        <property type="entry name" value="Calc_CGRP_IAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="51"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="166" mass="18111" checksum="CC4D98E821F08BAB" modified="2018-12-05" version="1">MLQSRLAAIKGVPKSLLNAAYLYKAPPWKLPFPELGEPRICKASYPNSPPGVSDLSAAISNPIPQEDTQHTGRAPPHRDPSSKLSDMLLHGNNNALPVEPVWQRGSRGRRHASSGSSRGHGHLMRVGCVLGTCQVQNLSHRLYQLIGQNGREDFSPINPRSPHSYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KKB7</accession>
    <name>A0A3B5KKB7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable N-acetyltransferase 14</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Probable acetyltransferase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the camello family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KKB7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000628"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000638.1">
        <property type="protein sequence ID" value="ENSXCOP00000000628.1"/>
        <property type="gene ID" value="ENSXCOG00000000547.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182932"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050769">
        <property type="entry name" value="NAT_camello-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13947">
        <property type="entry name" value="GNAT FAMILY N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13947:SF51">
        <property type="entry name" value="N-ACETYLTRANSFERASE 14-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="12">
        <location>
            <begin position="103"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="263" mass="30152" checksum="19324D507C8F6701" modified="2018-12-05" version="1">MVRLELDQVVMRRMKEDDIEVVKALVKEGCEGTENRLILHILTRPLCLFILAVFSSILRCLVHSFILALAIPVFLLIVFLKITMPRSTGVLGSSRPYWDYVGSSYRGQQDETLQNPYSSQANRRANKDKETSSEKITAEREQAAGQVWVADCEGDIVGCVFRESETRPSVRRICRLVTGCWYRREGLGRLLVQSLELKERESGARRVYAHIPYPSKVGEAFFRKVGYRQPGEADEEDDDEMKNETPERGFMGYPLTKVFYKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRJ9</accession>
    <name>A0A3B5KRJ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Insulin-induced gene protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Mediates feedback control of cholesterol synthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the INSIG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRJ9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002738"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002775.1">
        <property type="protein sequence ID" value="ENSXCOP00000002738.1"/>
        <property type="gene ID" value="ENSXCOG00000002182.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00580000081600"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032937">
        <property type="term" value="C:SREBP-SCAP-Insig complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032869">
        <property type="term" value="P:cellular response to insulin stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036316">
        <property type="term" value="P:SREBP-SCAP complex retention in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025929">
        <property type="entry name" value="INSIG_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15301">
        <property type="entry name" value="INSULIN-INDUCED GENE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15301:SF10">
        <property type="entry name" value="INSULIN-INDUCED GENE 2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07281">
        <property type="entry name" value="INSIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="5 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="10" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="136"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU241113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="230" mass="25794" checksum="6AC5090F2AD52379" modified="2018-12-05" version="1">MSNSALTSDQRSERQHNSRGPYVSCITNRTTNLLIRGGMLFFVGVFLALVLNLLQVQRNVTLFPPDVISSIFSSVWWVPPSCGIASAMIGVLYPCIDRRLGEPHKFKREWSSVMRCVAVFVGINHASAKVDFANNVQLSLTLAALSIGLWWTFDRSRSGFGLGISIALLATLTTQILVYNGVFQYTNPDFLYIRSWLPCIFFAGVITMGNIGRQLALYEYKFLQEKTHLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRP4</accession>
    <name>A0A3B5KRP4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ankyrin repeat and SOCS box containing 4</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRP4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000560"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000569.1">
        <property type="protein sequence ID" value="ENSXCOP00000000560.1"/>
        <property type="gene ID" value="ENSXCOG00000000485.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158874"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03723">
        <property type="entry name" value="SOCS_ASB4_ASB18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050745">
        <property type="entry name" value="Multifunctional_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189:SF71">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189">
        <property type="entry name" value="MYOTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017366281" evidence="5">
        <location>
            <begin position="22"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="133"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="166"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="201"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="278"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="6">
        <location>
            <begin position="406"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="453" mass="50750" checksum="196F7CD85D25E57A" modified="2018-12-05" version="1">MIISNLIHALWIIIDSEEVLGGQSDFLQDCMEELSPRQMAVKELKEKFLKALQENNAEEVLQILNTGKLDIDTVLEVEDPSMLLASYKQGYWLPGYKLEKSWAMGIHVCVMYNAVETALVLLQKGAAINSMPNGKSPLHVACEVSNSDCVALLLAYGAKVNSLSLSGHTPLHYCITRESVECARQLILKGAKVNTASHNNEEDTPLHTAARFGVPELVALLLTHRASVNTLNALQETPLMTAAFWALDSKEQVYSQDHHFVCRLLLDHQADPNLKEGDNKTALHMAAWNCDHVLMQMLLEAGADVRAMDINGCAPIQYVLKVTGVRPMAIPEVCFQLLLNYNAARIYPPQFHKVLQSCYDYPQVVEILVNSYEHLKPTRKWRGAIPDDCYERHKGFYDSMFAVCTNTPRSLLHLTRCAVRASLRGFCQQGVDQLPLPSTMKKYLLLEPEGILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KVB8</accession>
    <name>A0A3B5KVB8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inactive ADP-ribosyltransferase ARH2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ADP-ribosylhydrolase-like protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">[Protein ADP-ribosylarginine] hydrolase-like protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Required for myofibril assembly and outgrowth of the cardiac chambers in the developing heart. Appears to be catalytically inactive, showing no activity against O-acetyl-ADP-ribose.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ADP-ribosylglycohydrolase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KVB8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002467"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002501.1">
        <property type="protein sequence ID" value="ENSXCOP00000002467.1"/>
        <property type="gene ID" value="ENSXCOG00000001970.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063627"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030017">
        <property type="term" value="C:sarcomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003875">
        <property type="term" value="F:ADP-ribosylarginine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051725">
        <property type="term" value="P:protein de-ADP-ribosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4080.10:FF:000002">
        <property type="entry name" value="ADP-ribosylarginine hydrolase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="entry name" value="ADP-ribosylation/Crystallin J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012108">
        <property type="entry name" value="ADP-ribosylarg_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050792">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222">
        <property type="entry name" value="ADP-RIBOSYLGLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222:SF23">
        <property type="entry name" value="INACTIVE ADP-RIBOSYLTRANSFERASE ARH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016939">
        <property type="entry name" value="ADP_ribslarg_hdr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR605502-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="358" mass="40378" checksum="F2ADB194F31D04BE" modified="2018-12-05" version="1">MEKFKAAMVLGAVGDALGYRKGRWESCTSGKKIQEELASLGGLESLKLDPDNWPLSDAVLMHMTTGEALITDYWCLEDLYREVVRLYVEAMVSLQGRAPDPGTVEPCVHLKPHNFLLAWHTPFNEKGSGFGAAAKAMCVGMRYWQPERLDNLVEVSIEIGRMTHNHPTGFLGSLTTALFASFAIQGRPLVTWGRELMEVIPRAEEYCKKTIRHMAEYQESWFYFEAKWQFYLEEREIDKDGKNKPSFPDGYDAEETDKMYKRWSSEGRAGRRGHDAPMIAYDALLASGSNWAELCKRAMFHGGESEATGLIAGCLYGLINGLSQVPQNLHRDLDKRERLEELGEALYKAASAEKCTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KVM0</accession>
    <name>A0A3B5KVM0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KVM0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004168"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004214.1">
        <property type="protein sequence ID" value="ENSXCOP00000004168.1"/>
        <property type="gene ID" value="ENSXCOG00000003279.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165100"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="109" mass="11549" checksum="CCD31FBC4E071F18" modified="2018-12-05" version="1">MDPIVEVVALILGFVGWVMVGVALPNRYWKVSTVDGSVITTSTIYENLWMSCATDSTGVHNCREFPSLLALNGSVSPSTGSSCHYTASCTVCVSGSDSVEQTELLVSVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KX14</accession>
    <name>A0A3B5KX14_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mediator of RNA polymerase II transcription subunit 21</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Mediator complex subunit 21 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KX14"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000114"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000115.1">
        <property type="protein sequence ID" value="ENSXCOP00000000114.1"/>
        <property type="gene ID" value="ENSXCOG00000000108.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165964"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.10">
        <property type="entry name" value="Mediator complex, subunit Med21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021384">
        <property type="entry name" value="Mediator_Med21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13381:SF0">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13381">
        <property type="entry name" value="RNA POLYMERASE II HOLOENZYME COMPONENT SRB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="130" mass="14796" checksum="619A197FC6225CBF" modified="2018-12-05" version="1">MFPKFLPVKIWRVWHAFLFKTFNQITLRSTVCHCKSNQGCNRLIKVQVKIYKRRLKPDAAVDSAALSTCRQAASLRQLEEENHDAAARLEEVVYRGDMLLEKIQNALADIAQSQLRTRNGALSQPSPAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KX68</accession>
    <name>A0A3B5KX68_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tripartite motif containing 13</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027880114.1">
        <property type="nucleotide sequence ID" value="XM_028024313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KX68"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002226"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002257.1">
        <property type="protein sequence ID" value="ENSXCOP00000002226.1"/>
        <property type="gene ID" value="ENSXCOG00000001778.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114148830"/>
    <dbReference type="GeneTree" id="ENSGT00940000159715"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
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        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
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    </dbReference>
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        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="10"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="7">
        <location>
            <begin position="87"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="395" mass="44001" checksum="C769AD307B930512" modified="2018-12-05" version="1">MEQLEEELTCPVCCGLFEDPRVLLCSHSFCKKCLEGLLEGNRGPAFRTPLKCPTCRKETPHNGASSLQVNYSLRGIVDKFSKIKVMPKMSACKQHCGQPLNIFCATDLRLICGICVTTDEHRGHGFSSLEEAYEREKGAFGGLLQEAESWQSADTLSCLETLQASKKKALQSVGRDAEKVADYFDKLIGSLECKKSEILSDFETLKLVVMQAYDPEISKLSAALEEQSRALGLAESVRGVSDPLCFLQNMQEFREKIKVLKETALPSRKDMDVGPLVRNFDVKKWDSLRLREIDKISVPHESGAYQTGGSRMSWSKWIVLSLAFLSTLPIIFLSPPYTAIDVTSRIPALITESVPSATLYLQEISDMCAGLIEAGQEHMMRFISDLSTTFTHYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KY30</accession>
    <name>A0A3B5KY30_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KY30"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005013"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005073.1">
        <property type="protein sequence ID" value="ENSXCOP00000005013.1"/>
        <property type="gene ID" value="ENSXCOG00000003932.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155699"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006398">
        <property type="term" value="P:mRNA 3'-end processing by stem-loop binding and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF11">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="26"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="142" mass="16303" checksum="4F94B6AFCA85C4D8" modified="2018-12-05" version="1">MSAYMVVPAEESDQLLIRPLGAGQEVGRSCIILEFKGRKIMLDCGIHPGLEGMDALPYIDLIDPAEIDLLLISHFHLDHCGALPWFLQKTSFKGRTFMTHATKAIYRWLLSDYVKVRWEQPCYANMLHFRAKAFTMFVRLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYR1</accession>
    <name>A0A3B5KYR1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="6">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="30 31">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="30" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005238"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005299.1">
        <property type="protein sequence ID" value="ENSXCOP00000005238.1"/>
        <property type="gene ID" value="ENSXCOG00000004096.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234517"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072518">
        <property type="term" value="F:Rho-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901888">
        <property type="term" value="P:regulation of cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd20875">
        <property type="entry name" value="C1_ROCK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01242">
        <property type="entry name" value="PH_ROCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22250">
        <property type="entry name" value="ROCK_SBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05596">
        <property type="entry name" value="STKc_ROCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000047">
        <property type="entry name" value="Rho-associated protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000308">
        <property type="entry name" value="Rho-associated protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000036">
        <property type="entry name" value="Rho-associated protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.730:FF:000001">
        <property type="entry name" value="rho-associated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000072">
        <property type="entry name" value="Rho-associated protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.730">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057529">
        <property type="entry name" value="MRCK/ROCK_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015008">
        <property type="entry name" value="ROCK_Rho-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF28">
        <property type="entry name" value="RHO-ASSOCIATED PROTEIN KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25346">
        <property type="entry name" value="PH_MRCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08912">
        <property type="entry name" value="Rho_Binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103652">
        <property type="entry name" value="G protein-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51859">
        <property type="entry name" value="RHO_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="19 22 24" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="21" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-0418">Kinase</keyword>
    <keyword evidence="18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="16" id="KW-0862">Zinc</keyword>
    <keyword evidence="14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="68"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="29">
        <location>
            <begin position="445"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="RhoBD" evidence="28">
        <location>
            <begin position="934"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="27">
        <location>
            <begin position="1219"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1308"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="24">
        <location>
            <begin position="41"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="24">
        <location>
            <begin position="395"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="24">
        <location>
            <begin position="909"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="24">
        <location>
            <begin position="1008"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="1329"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="25">
        <location>
            <begin position="1357"/>
            <end position="1368"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1368" mass="158615" checksum="093AB2AA078EBDD4" modified="2018-12-05" version="1">MKEVELQSFKLTFVKLILHFLLIDSMNALALDLNYPVLRKNKNIEAFLDRYEKLVNELQELQVKLDDFERVKLIGRGAYGEVQLVRHKASKKVYAMKQLSKFEMIKRSDSAFFWEERHIMAFSNSPWIVQLCCAFQDDRNLYMVMEFMPGGDVVTLTINYDIPEKWARFYTAELVLALDAIHSMGFIHRDIKPDNMLLDQHGHLKLADFGTCMRMNSSGMVHCETAVGTPDYISPEVLQSQGGDGTYGRECDWWSVGVSLYELLVGETPFYAESLIGTYGKIMNHHNALVFPDDVEMSQNAKDLISAFLTDRKVRLGRTGVDEIKCHPFFKDDQWTFDNIRDTVAPVVPELNSDIDTSNFDEIEVEKGTAETFPPPRAFAGNQLPFIDIFLCFSSEQLQKKVKQLEEQLDHEMQAKDELEQKCRCKFAFAFLFYQMNSRQKVEASMRQLERERALLQHQSVENQRKVEMEADRKRILENELNNLRDQLEDQKKRNQHSQISNEKNIQLQKQLNEANDALQAEQEAVTRLKRSQAETQKQAQSLEVSLREMEKKCSGLEASKAEQEKQMRGLQVELEEERGEHNLRTETLADLQGTISLLEDEVKEVKSSLSKVNSEKKELQTKLNNLEKEKNNQAIDLAFQLKSLQQSLEQEEAEHKATKAKLADKSEIYKSIEEAKSEALKGMESTLQEERYLKLQVEEQLLQLKKEHSMLDCDHKQAEQKLEELQAQKEKLSEEVMDLTLRLEQETRKRSLSQSDLKAQKQQVSALASSEKQLKQELNQLLDMKQSLEKQNQKLHREKQEADGQLKELKDQLECICQTVATAEYFTTLYKTQIRELKDECNEKNKMYKEAQQQLAEYQEERDSLAAQLESSLTKADSEQLARSIAEEQYSLLEKEKIMKELEIKDMMARHKQELSEKEATIGSLEESNRTLTVDVANLASEKEELNNQLKDLQQELQKAKELKKELESLKVSFERQVQTERTLKIQAVNKLAEVMNQKKEKGRGGVRAVDSEVHKKEKENRKLQLELKAERQNLNSIIFKYQKELDDMQAMMVEETHMRQELQMALDSKDSDIERLRCQITSLSVHSLDSTSISSNNDLEMGDGFPDSRLEDWISLPSKNTKRFGWDKKYVVVSSKKILFYNSQMDQEQANPFMTLDIEKLFHVRPITQTDCYRADVKEIPRIFQILYANEGESKRDQEAVEEPAPCERSNYIMHKGHEFINTLYHLPCSCEVCVRPLWNVFKPPAALECRCCHTKYHKDHLDKKEDNIPPCKVNYDMSTAKELLLLTSSQTEQQRWVNHLLKRIPKKNPTLSPPPAARNSPIESLPFSSPHMSPESSPKDSPRLSHRRAIKIPSTRQQQTSDKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZC8</accession>
    <name>A0A3B5KZC8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027890392.1">
        <property type="nucleotide sequence ID" value="XM_028034591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZC8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003405"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003443.1">
        <property type="protein sequence ID" value="ENSXCOP00000003405.1"/>
        <property type="gene ID" value="ENSXCOG00000002685.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114155006"/>
    <dbReference type="KEGG" id="xco:114155006"/>
    <dbReference type="CTD" id="406559"/>
    <dbReference type="GeneTree" id="ENSGT00940000161769"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061371">
        <property type="term" value="P:determination of heart left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061028">
        <property type="term" value="P:establishment of endothelial barrier"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070121">
        <property type="term" value="P:Kupffer's vesicle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000001">
        <property type="entry name" value="Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="215" mass="22918" checksum="3EF9F7829F0F389B" modified="2018-12-05" version="1">MVSAGLEILGLSLCVIGSLLVMVACGLPMWKVTAFIEANIVVAQTMWDGLWMSCVVQSTGQMQCKVHDSILALSHDLQAARALTIISSVMGVLGLMVVIAGAQCTNCIRTEYVKARVVNAGGIIYIISGLFVLVPLCWMANNIISDFYNPQVPSSKKREIGAALYIGWAAAALLLIGGTLLCCSCPSSGNSGYSVKYAPTKRASQNGDYDKRNYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZK6</accession>
    <name>A0A3B5KZK6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the large ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZK6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003987"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004031.1">
        <property type="protein sequence ID" value="ENSXCOP00000003987.1"/>
        <property type="gene ID" value="ENSXCOG00000003136.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00910000144184"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="entry name" value="Ribosomal protein l15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_eL15_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_eL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01384">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="73" mass="8971" checksum="3817819922C24EFF" modified="2018-12-05" version="1">MGAYRYMQELWRKKQSDVMRFLLRVRCWQYRQLSNLHRAPRPTRPDKARRLGYKAKQGGYFPMDLMLTAHHRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L138</accession>
    <name>A0A3B5L138_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L138"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003635.1">
        <property type="protein sequence ID" value="ENSXCOP00000003596.1"/>
        <property type="gene ID" value="ENSXCOG00000002838.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000176908"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="98" mass="11050" checksum="E3D3258A8A933778" modified="2018-12-05" version="1">MAPVGESQVKHLVTTLELPLPPQELISGDRLPLSAGQAGSQHGIVGQMQEDLQNQAVRQTWTPIHPNILEHTQRCEQVLSYYRFLQGFFCHLKGFRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L1Q2</accession>
    <name>A0A3B5L1Q2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Brambleberry</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L1Q2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004727"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004784.1">
        <property type="protein sequence ID" value="ENSXCOP00000004727.1"/>
        <property type="gene ID" value="ENSXCOG00000003721.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00740000115898"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040346">
        <property type="entry name" value="GEX1/Brambleberry"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33538:SF1">
        <property type="entry name" value="PROTEIN BRAMBLEBERRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33538">
        <property type="entry name" value="PROTEIN GAMETE EXPRESSED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017433611" description="Brambleberry" evidence="2">
        <location>
            <begin position="18"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="346"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="55484" checksum="792262E4B840ED1C" modified="2018-12-05" version="1">SFFWFVILFVFPFSGLFEWLRTPQPPSTVAPPPVAPLLLAKDAQFEMVTSDEKFLAEAKQLELSPLDSCHYRVVARLRASCESLSEENLAKLGVELFNCQAEIEGRRSFPCTQEMTIKECTEDMDPDTWNAYHIVSNRARSVCYAARQQLFRRRAELTVNSLISTASSQLDAMKDLKVGQLELKELAAASLDKLLEGHSTLQAQQGKLHEGQEQMEGSLRENLERLGQEKALIASGQELVAQLIQGIAKGMENMSEHLQIQSSEVQDGHKAITDDLAEVRHQAQDIYQKIDHSMSEFLQYQDQTSQYYTDLMTKLERMNSTLGVMLHYLDNMEVRIEERLHMIQSYLGWAGLSLTAMWTCVAHTAYFVLCAVLLTFLRCPGFSRAMLLLMVPLNAVAEVNQQPALDLTSLSVLLLAFSLGTTFNPGTSQIRANDSHDFGKDSRSASPTLSIITNSSLSGRFLCNGITKAGKACKKKAVPGQEYCHVHEGGHSSYVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2M3</accession>
    <name>A0A3B5L2M3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Discs, large (Drosophila) homolog-associated protein 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAPAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2M3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002034"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002064.1">
        <property type="protein sequence ID" value="ENSXCOP00000002034.1"/>
        <property type="gene ID" value="ENSXCOG00000001631.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159513"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099572">
        <property type="term" value="C:postsynaptic specialization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023052">
        <property type="term" value="P:signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005026">
        <property type="entry name" value="SAPAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12353:SF4">
        <property type="entry name" value="DISKS LARGE-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12353">
        <property type="entry name" value="DISKS LARGE-ASSOCIATED PROTEIN DAP SAP90/PSD-95-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03359">
        <property type="entry name" value="GKAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="164"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="398"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="527"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="853"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="77"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="173"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="218"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="406"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="546"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="853"/>
            <end position="866"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="965" mass="104430" checksum="8B9996E1B5F9DE88" modified="2018-12-05" version="1">MKGYHVSRSMSQHSSGGGGGGPCHCTPDDCDGPGRDYYHGRSDGYYYSGGPTEALALERHHSHSHSHAHSHSHSHSGTFPRSHPSQHPPLQSFDSCEDCLSSGHGGKMHRIPPNLMDQFEKQVPFHPDGFHTLQYQRTASGGAEHRSESPSRIRHLVNSVQRLFAKSHSLEAPTKREYNGTRGGGEYRGERGGGHRSGGEEVHYSGHQSRSTRRSKSRERSKSGDSRHESGRRHRSRTAGWWSSDDNLDSDSSFLVSGGRRGYPTGHESLDAAIQELTMKKPKERSGGPGSSECMACTTMALTGGEGGGHHGHHSHSLKRSTWSAMTVSQAREVYPSTRGGGYDKALVPLENKLKERSFHYLQVPSEDWGGGGYGGSADSGGEIPCRRMRSGSYIKAMGDDDSADSETSPKASPKSTLIAQRDAFRRSISMDYSCKQCTDSYPNSRTAPKTHTRSRSYTRSLTSSQFETVCETMFGEVESQAVEALDLPGVFRTRSHSYVRAIQAGCSQDDDCLSVFSMSAPQGSIKGGAFPYRKGAPPPLPPRMSKSSLSVRAQSSTESTQDAYFQSGGQMAPGTGRPKQHSNSIDLGGSEGPSGRASRGGYYTSGGPGRSRQHSNSAESLDGVKGSRELVPYGGGPGVGVRAKHSCSADSLLEGPPRPARDRDGRVGGGSLGKSSSLPQNNMIVSKIGGQDDGRAARKWRPSIAVQVDSSETLSDSDTEGKALTEVHSIGVQVEDEKRARFKRSNSVTASVQADLDPEGFPGLSVAVPTQDKSLQFGCSFQRHSSEPESASQYTECHRTVHTQGQWAYREDPSAYPQPTSQDLASFWDLLQLNIEDVRVRFQDLQKLKDSGWRLPPEKKEDKKLPPPLPKKPAGGVSGSLRADSVGDGVSVSARGGPAGLVVPRVGGHTLPIREKSLDLGDRQRTEARRRLLQTKRTASFRQNSATESADSIEIYIPEAQTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4Y1</accession>
    <name>A0A3B5L4Y1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NACHT LRR and PYD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSXCOT00000004945.1">
        <property type="protein sequence ID" value="ENSXCOP00000004886.1"/>
        <property type="gene ID" value="ENSXCOG00000003829.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286915"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051261">
        <property type="entry name" value="NLR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24106">
        <property type="entry name" value="NACHT, LRR AND CARD DOMAINS-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="NACHT LRR and PYD" evidence="3">
        <location>
            <begin position="40"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="427" mass="47439" checksum="80951A1A3F4A11DC" modified="2018-12-05" version="1">MLVTVLSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFLVGLSLKTNQRLLKGLISPTESKTSQDTALYIRKKISENLSGEKSINLFHCLNELGDCSLVEEIQLSLSSRSHLKENLSPAQWSALGFILLSSEKDLDVFDLKKYSASEDALLRLLPVVKASKTAILSDCDLTERSCEALAPVIGDHWSSLRELDLSNNKLLDSGVKMLWSGLESENPQLEALRLSFCNLTEKSCESLASVLSIQSSSLRELDLSNNDLQDSGVKFLSAGLKSPHCKLETLRIISFTFLCGLTSVYFVKFQMIQTYLHSGMVGRSCAEVQQNLIYLQTVAPFYAIWMPSMLFCVCRLSGCLITEDSSVSLASALTSNPSSLVELDLSFNNPGEKAKKLLRERQEDPHCELKHLRYGEHQIGLISKLKCFVDLVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6A9</accession>
    <name>A0A3B5L6A9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lin-9 DREAM MuvB core complex component</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lin-9 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6A9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005940"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006006.1">
        <property type="protein sequence ID" value="ENSXCOP00000005940.1"/>
        <property type="gene ID" value="ENSXCOG00000004609.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003188"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033471">
        <property type="entry name" value="DIRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010561">
        <property type="entry name" value="LIN-9/ALY1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045831">
        <property type="entry name" value="LIN9_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21689">
        <property type="entry name" value="LIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21689:SF2">
        <property type="entry name" value="PROTEIN LIN-9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06584">
        <property type="entry name" value="DIRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19438">
        <property type="entry name" value="LIN9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01135">
        <property type="entry name" value="DIRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017393032" evidence="4">
        <location>
            <begin position="22"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="DIRP" evidence="5">
        <location>
            <begin position="51"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="467" mass="53023" checksum="A85339CE3742927B" modified="2018-12-05" version="1">MFTYCVCILLVLQKFSNIVTPDKKASQKIGLRLRNLLKLPKAHKWCIYEWFYSNIDRPLFEGDNEFCLCLKESFPNLKTRKLTRVEWGTIRRLMGKPRRCSSAFFAEERTALRQKRQKMRLLQQRKLSDVSTCKDLPDEIPLPLIIGTKVTARLRGVHDGLFTGQIDAVDTSAATYRVTFDRSGLGTHTVPDYEVLSNEPNETMPISAFAQKHRSTRYIQNLMTPPRAPYPSITTPDNDPLMNASPWRSKLPSTEGETLGGFPVKFLVQVTRLSKILMIKKEHIKHLKEMNTEAEKLKSYSMPIDLDFQKRYATTVLELEQLNKDLNKVLHEVQQFCCELSPDQGMVPADHPTDLRRRCEEEAQQMVQLSNTQRDGQPTVTNASLTHLVSRLTALLLQIKCLADGGDLNSFEFKSLTDSLNDIKASIDSSNLSCFQNNVEIHVAHIQSGLSQLGNLHAFAANNTNAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6Q3</accession>
    <name>A0A3B5L6Q3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Pseudouridylate synthase 1 homolog</fullName>
            <ecNumber evidence="12">5.4.99.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">tRNA pseudouridine synthase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">tRNA pseudouridine(38-40) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">tRNA pseudouridylate synthase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">tRNA-uridine isomerase I</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Pseudouridylate synthase that catalyzes pseudouridylation of tRNAs and mRNAs. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3'. Acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions. Pseudouridylation of pre-mRNAs near splice sites directly regulates mRNA splicing and mRNA 3'-end processing. Involved in regulation of nuclear receptor activity through pseudouridylation of SRA1 mRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a uridine in tRNA = a pseudouridine in tRNA</text>
            <dbReference type="Rhea" id="RHEA:54572"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13934"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:22376"/>
            <dbReference type="Rhea" id="RHEA-COMP:10085"/>
            <dbReference type="Rhea" id="RHEA-COMP:10087"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer. Forms a complex with RARG and the SRA1 RNA in the nucleus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.12" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6Q3"/>
    <dbReference type="SMR" id="A0A3B5L6Q3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006120"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006189.1">
        <property type="protein sequence ID" value="ENSXCOP00000006120.1"/>
        <property type="gene ID" value="ENSXCOG00000004722.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183160"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160147">
        <property type="term" value="F:tRNA pseudouridine(38-40) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02568">
        <property type="entry name" value="PseudoU_synth_PUS1_PUS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.580:FF:000002">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.660:FF:000002">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041708">
        <property type="entry name" value="PUS1/PUS2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF4">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="21">
        <location>
            <begin position="216"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="29"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="381"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="18">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00075153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00079087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00080849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR641708-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR641708-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="402" mass="45717" checksum="49C213299A7B3178" modified="2018-12-05" version="1">MYSAIFTGLKFLCTMSEEARDQETAKLLKRPNEVNEDSAERATKRIKAEGEHNEDDKRYPKKKVVLLLAYSGKGYYGMQRNPGNSQFRTIEDDLVTALVKSGCIPGNHGDDMRKMSFQRCARTDKGVSAAGQVVSLKLRLIEDIIEKINENLPPQIRVLGLKRVTQGFNSKNNCDARTYAYMLPTVAFSPKDYDTENIAAFRLETETLQKVNQLFALYKGTHNFHNFTSQKAPSDPSARRYITEMSCGEPFVRNNYEFAVITVRGQSFMLHQIRKMIGLVIAVIKGYAKEEVMERSWGQEKVDVPKAPGLGLVLERVHFDRYNKRFGGDGLHERLEWDREEEAIKAFKEAHIYPTIVETECEEGSMVSWMATLPIHDFEATVTGTQDNKDQKQNADEGNDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L6U9</accession>
    <name>A0A3B5L6U9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Solute carrier family 25 member 36a</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L6U9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006165"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006236.1">
        <property type="protein sequence ID" value="ENSXCOP00000006165.1"/>
        <property type="gene ID" value="ENSXCOG00000004762.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154369"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015218">
        <property type="term" value="F:pyrimidine nucleotide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990519">
        <property type="term" value="P:pyrimidine nucleotide import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000124">
        <property type="entry name" value="Solute carrier family 25 member 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049562">
        <property type="entry name" value="SLC25A33/36-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829">
        <property type="entry name" value="MITOCHONDRIAL CARRIER PROTEIN RIM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45829:SF2">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="90"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="198"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="285" mass="31595" checksum="41C2708EDAE22404" modified="2018-12-05" version="1">MSQRDTLVHLFAGGCGGTVGAILTCPLEVVKTRLQSSSITLYVSEVQLSTVNGPSVTRVSPPGPLHCLKAIYFAAYSTAKEKLNAVLEPDSTQVHMLSAGMAGFTAITATNPIWLIKTRLQLDARNRGERRMSALECVRRVYKADGFRGFYRGMSASYAGISETVIHFVIYENIKRRLLEAKTPQNMDEEEEASKDASDFVGMMLAAATSKTCATTIAYPHEVIRTRLREEGTKYRSFFQTLATVPREEGYRALYRGLATHLVRQIPNTAIMMCTYELTVYLLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L720</accession>
    <name>A0A3B5L720_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Gamma-aminobutyric acid type A receptor subunit alpha5</fullName>
        </submittedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Postsynaptic cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L720"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008103"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008201.1">
        <property type="protein sequence ID" value="ENSXCOP00000008103.1"/>
        <property type="gene ID" value="ENSXCOG00000006207.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156234"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19038">
        <property type="entry name" value="LGIC_ECD_GABAAR_A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19052">
        <property type="entry name" value="LGIC_TM_GABAAR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000001">
        <property type="entry name" value="Gamma-aminobutyric acid A receptor subunit alpha-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000002">
        <property type="entry name" value="Putative gamma-aminobutyric acid receptor subunit alpha-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001390">
        <property type="entry name" value="GABAAa_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047024">
        <property type="entry name" value="Gabra-1-6_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01079">
        <property type="entry name" value="GABAARALPHA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0868">Chloride</keyword>
    <keyword evidence="11" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="274"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="440"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="19">
        <location>
            <begin position="67"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="20">
        <location>
            <begin position="281"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="468" mass="52662" checksum="4F71CF5E0D4B3695" modified="2018-12-05" version="1">MSAFISSSWIVERWFESFLHVSLDSSLFLSPSLHPSLPPSSSSPSPHTSSSGTPKQAEINDNITIFTRILDGLLDGYDNRLRPGLGEKVTEIKTNIFVTSFGPVSDTEMEYTIDVFFRQSWKDERLCFKGPMEMLALNNLLASNIWTPDTFFLNGKKSIAHNMTTPNKLLRLKDDGTLLYTMRLTISAECPMQLEDFPMDAHACPLKFGSYAYPVSEVIYKWTEDAAKSVVVAEEGSRLNQYHLIGQTAGTEEIYTSRGEYTVMMAHFYLKRKIGYFVIQTYMPCFMTVILSQVSFWLNRESVPARTVFGVTTVLTMTTLSISARNSLPKVAYATAMDWFIAVCYAFVFSALIEFATVNYFTKRSWAWDGKKKKDPLALSMKPNNTCSAGVNYTANLTKDASISTISNSTTIQLKPSESKAPDPKKTYNSVSKIDKMSRIVFPVLFGTFNLVYWATYLNREPVIKGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L890</accession>
    <name>A0A3B5L890_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L890"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007037"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007125.1">
        <property type="protein sequence ID" value="ENSXCOP00000007037.1"/>
        <property type="gene ID" value="ENSXCOG00000005436.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155369"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF164">
        <property type="entry name" value="MAM DOMAIN-CONTAINING GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="29"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="134"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="305" mass="33947" checksum="9AA2AC342A3A109D" modified="2018-12-05" version="1">MWENSLHGRHLVFQNTDHNYVFSLSSVPPELSVPKGQSQLIVLEGDTVDLQCLVSGKPKPIIMWSRVEESGQTAALAPTMPDGSVQTESYDGILRIYNVTRKMSGAYRCQTSQYNGFNVKPREALIHLVVQFTPTVKPVYTEVRQALGRAFSLTCNLLEAYPAHHLQYEWKLGSRLLTVGQFSDQSHDTSYHVKALNREGYGEYTCDITNKAGTGRCTFLLTGDLLNVTGSQSESADSPLRFLRGNYIGESQTADMAQPEVQRTSKMICGNNINVYSIFRAKNIEMTRGGVGAKLLPRNFSAVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8F3</accession>
    <name>A0A3B5L8F3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glutamine-rich 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8F3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006109.1">
        <property type="protein sequence ID" value="ENSXCOP00000006041.1"/>
        <property type="gene ID" value="ENSXCOG00000004680.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155241"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057926">
        <property type="entry name" value="QRICH1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021893">
        <property type="entry name" value="ZMYM2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051284">
        <property type="entry name" value="ZnF_MYMT-QRICH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45736:SF8">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR QRICH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45736">
        <property type="entry name" value="ZINC FINGER MYM-TYPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12012">
        <property type="entry name" value="DUF3504"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25561">
        <property type="entry name" value="QRICH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="QRICH1-like" evidence="6">
        <location>
            <begin position="412"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="ZMYM2-like/QRICH1 C-terminal" evidence="5">
        <location>
            <begin position="539"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="714" mass="79670" checksum="EC6EF49433C5F3E5" modified="2018-12-05" version="1">MNEQEGGVVSFDEYVRQKARTVPQHRMKEFLESLAKGPEVLQEFNQQGGGGGAVATTTTMVYQQQEANCIYTDSTEVAGSLLELACPVRLCCIYINVFFVFSQVQVTSAEISPQMSVHPGSEQQLQVQVGLYILIFKMQLISGEQIQLQGTQHIQLPGGQQIQLQAGQQIQLQSGQHIQIQTIEAMSPSQQQDSPREGERRPGAIATVLQPAKKRKVDVPLAVSYAVPQGQQLATVLTIPQGQQQSYVSLRPDLLTVDSAQLYSTTGTITGPTGETWTIPVYSTPQQQGVTHIAIPQDAYSTVQVTTTTNGKDKVATSSASRSADGQVASTQEEIVQTLLPAQFMNGNIHIPVAVQTVGGTYNTTQSVHIWDPSQQQSHGDEGTEQQLHLQGQVETGADVEPPAEILVPVSLKPEEGLEVWRFWAKKKNAELSKQEQTKLAPIGRRQPLRFQEDLVSSAVAELNLGLSLMTKEARGSEEEELAPDVLYYVFLCIQKYLYENGRVDDIFSDPYYTRFCESLHKLLQDWKPSIHPLGYIIPSHVTEEMLWECKQLGAHSPATLLTTLMYFNTKYFRLITPEHHMKVAFSKVLRHSRKNPTNAKDKATSIRLLKVQSQHTSGQKGTDDMYEEQIEDPENPLRCPIKLYDFYLFKCPQSVKGRSDAYYMTPEPVVAPNSPMWYSSQPLSNRQVEQMLARIIVVREIQEMMGTSPESVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LA23</accession>
    <name>A0A3B5LA23_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cystatin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cystatin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LA23"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006671.1">
        <property type="protein sequence ID" value="ENSXCOP00000006591.1"/>
        <property type="gene ID" value="ENSXCOG00000005104.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000176112"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031982">
        <property type="term" value="C:vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00042">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000010">
        <property type="entry name" value="Cystatin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046350">
        <property type="entry name" value="Cystatin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46186">
        <property type="entry name" value="CYSTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46186:SF13">
        <property type="entry name" value="SI:BUSM1-57F23.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00031">
        <property type="entry name" value="Cystatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00043">
        <property type="entry name" value="CY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54403">
        <property type="entry name" value="Cystatin/monellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cystatin" evidence="2">
        <location>
            <begin position="28"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="140" mass="15970" checksum="D8BDE65E00887FF0" modified="2018-12-05" version="1">GNITRHPLCSLPPMLTKAAHGLEVCMVSLLGSRWVWSSLTEDVQKAVEFAVEMFNKKAKGKMLFKLVSINSAKSQESSKIKYKIDAVFERTKCPKWKNHDVKSCILEKQQLKCQFVVSLNPRKSKYDLKSKKCHEVTKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LA75</accession>
    <name>A0A3B5LA75_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="19">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Component of a complex with RUVBL1 and PSMC5. Interacts with centrins CETN1, CETN2 and CETN3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LA75"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004764"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004822.1">
        <property type="protein sequence ID" value="ENSXCOP00000004764.1"/>
        <property type="gene ID" value="ENSXCOG00000003690.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157997"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000067">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000028">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF5">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="15" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 19" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 19" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="10 19" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="23">
        <location>
            <begin position="2"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="22">
        <location>
            <begin position="261"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="147"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00065368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="647" mass="74448" checksum="F8C1379BDF84340E" modified="2018-12-05" version="1">MDRCKHVVRLRLGQDHSILNPQKWHCVECSTTDSVWACLKCSHVACGRFIEEHFLKHFQESQHPLAMEVRELDVFCFACGDYVLNDNAEGDLKLLRGALSTVRSPGRRSLRSSAGGDCAPWVLEGGPQPAMQLALRHRRKVLLGKMLQTWIRKHQEVQNQRKEKLEEARRQKKEVKRKLLEDLGNIPPRKSARLLTQGPRSTMTLIPRKFRDPPERLPPPPKKPSLLALPRRAPQISRAAKLRRYYSTHAVTRRRHAPGVTGLRNLGNTCYMNSILQVLSHLQKFRECFLTLDLCETEELLAKTNHSQGMKGAAELVQSKEPRCSTRQQMSLCHELHTLFRVMWSGRWTLVSPFAMLHSVWNLIPAFRGYDQQDAQEFLCELLDKVQQELDTEGSKRRIVIPITKRKLSKQVLKVLNTIFHGQLLSQVTCLSCKHKSNTVEPFWDLSLEFPERYHSRNKGSGATAYQRSCSLIEMLSKFTEMEALEGNIYACNHCNKRRRKSSHKPLVLSEARKQLLIYRLPQVLRLHLKRFRWSGRNHREKIGVHVAFDQVLNIKPYCCTDSGHSVHRGGYTYDLSAVVMHHGKGFGSGHYTAYCYNTEGGFWVHCNDSEMKVCSVEEVCNTQAYILFYTQRADESQKADTTTTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBP8</accession>
    <name>A0A3B5LBP8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Complement component 1 Q subcomponent-binding protein, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Extracellular side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MAM33 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027888109.1">
        <property type="nucleotide sequence ID" value="XM_028032308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBP8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007136"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007228.1">
        <property type="protein sequence ID" value="ENSXCOP00000007136.1"/>
        <property type="gene ID" value="ENSXCOG00000004973.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114153634"/>
    <dbReference type="KEGG" id="xco:114153634"/>
    <dbReference type="CTD" id="708"/>
    <dbReference type="GeneTree" id="ENSGT00390000018406"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006958">
        <property type="term" value="P:complement activation, classical pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.280.10:FF:000001">
        <property type="entry name" value="Complement component 1 Q subcomponent-binding protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.280.10">
        <property type="entry name" value="Mitochondrial glycoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003428">
        <property type="entry name" value="MAM33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036561">
        <property type="entry name" value="MAM33_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826">
        <property type="entry name" value="COMPLEMENT COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826:SF1">
        <property type="entry name" value="COMPLEMENT COMPONENT 1 Q SUBCOMPONENT-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02330">
        <property type="entry name" value="MAM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54529">
        <property type="entry name" value="Mitochondrial glycoprotein MAM33-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="19" id="KW-0007">Acetylation</keyword>
    <keyword evidence="22" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="14" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0180">Complement pathway</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="16" id="KW-0391">Immunity</keyword>
    <keyword evidence="12" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="23" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="25" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="26" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0964">Secreted</keyword>
    <keyword evidence="24" id="KW-0804">Transcription</keyword>
    <keyword evidence="20" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="18" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00021918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="289" mass="32011" checksum="04606AB1DEB349C1" modified="2018-12-05" version="1">MLKSVVRAVGAAVRLSSAAPSAARALPLSYPALRSPGSATTRPFTRSLWMLNNNGASSGYRPKLLSSKVLNPSVSCGCGGLHTEGDKAFGDFLSDEIKEEKKIQKNKSLPKMSGGWELEMNGTEAKLTKQLSGEKITVTFNVNNSIPPNFEEEVEQGQQQKSPEDEPEIVSTPNFVIEVTKQGAKHSLVFDCHFPEDEMSHGEGEEESDIFAIREVSFQPEGDSDWKETSYTLNTDSLDWALYDHMMDFLADRGVDNTFADELTELSTAIEHKEYIKFLEDLQSFVNCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBU7</accession>
    <name>A0A3B5LBU7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Crumbs cell polarity complex component 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the Crumbs protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000008317"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008422.1">
        <property type="protein sequence ID" value="ENSXCOP00000008317.1"/>
        <property type="gene ID" value="ENSXCOG00000006369.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155152"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048592">
        <property type="term" value="P:eye morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051093">
        <property type="term" value="P:negative regulation of developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051241">
        <property type="term" value="P:negative regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050877">
        <property type="term" value="P:nervous system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003002">
        <property type="term" value="P:regionalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008593">
        <property type="term" value="P:regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000348">
        <property type="entry name" value="Crumbs 1, cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000081">
        <property type="entry name" value="Crumbs 1, cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000252">
        <property type="entry name" value="Crumbs 1, cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000208">
        <property type="entry name" value="Crumbs 2, cell polarity complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000039">
        <property type="entry name" value="Crumbs cell polarity complex component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000055">
        <property type="entry name" value="Crumbs cell polarity complex component 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000123">
        <property type="entry name" value="Crumbs homolog 1 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000327">
        <property type="entry name" value="neurogenic locus notch homolog protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000173">
        <property type="entry name" value="Neurogenic locus notch protein 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000565">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000143">
        <property type="entry name" value="Protein crumbs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000122">
        <property type="entry name" value="Protein crumbs homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013032">
        <property type="entry name" value="EGF-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051022">
        <property type="entry name" value="Notch_Cell-Fate_Det"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049">
        <property type="entry name" value="CRUMBS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24049:SF1">
        <property type="entry name" value="PROTEIN CRUMBS HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12661">
        <property type="entry name" value="hEGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="13 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 17" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1262"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="13"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="51"/>
            <end position="87"/>
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    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="110"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="148"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
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            <begin position="187"/>
            <end position="223"/>
        </location>
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    <feature type="domain" description="EGF-like" evidence="21">
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            <begin position="283"/>
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            <begin position="327"/>
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            <begin position="369"/>
            <end position="557"/>
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    </feature>
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            <begin position="601"/>
            <end position="774"/>
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            <end position="836"/>
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        <location>
            <begin position="865"/>
            <end position="1056"/>
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    </feature>
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        <location>
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    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="1240"/>
            <end position="1249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00060989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1324" mass="145250" checksum="F05BB2A3F59AB721" modified="2018-12-05" version="1">MLLGASPDKLSQTPDPCLQNLCQNQAICRTRGNGYSCFCVPGFQGAHCQIDVNECASQPCRNGATCVDGVGRFSCLCPPGFTGDLCSWAHLDSDICVALMMFTGTICELQVDQCQSQPCLHRGTCVNHATGFRCICHVGFQGDRCEINTDDCQEQPCQNGALCVDGVNEYSCDCSHTAFTGPHCETLLPQCNSDPCFNSAICKDNQGNYTCECWPGFEGRNCEIDTTECSSSPCMNRGRCLERSWQALYGSEPLLPERYDHRYAAGYICICPPGTAGFHCQEVVNQCESSPCQNGGRCESLDGGFTCQCLKQARDGVLYGGTNCDVKLVGCEGHECQNGASCSPFLLDGTDGYTCSCTPGYTGPLCNTPTTFSFERRGYLLLHNPLVNADLTTNITLSFKTVLPSALLFQRNIRGMTLQLQLDRGQLFLMLWSEKSVGSDGEIHTLELPHNVMDGEWHTVEVVLTNEILSLRLFDDAGRYGRQLYHTVTPVQTNLTELETSPQDTFIGGGLKNPHWFSRHFSLPVFIGCMRDVFVDWQLVVPEEWLSDSAVNVSPGCSHRDRCRNEPCQNGGRCENLWQSYQCQCTRPYEGHDCEEEHVTARFGNEDSHSFAAFAIHDDLGQNLSVSLFLRTRRRNGLLLVLSNSSSSPYLHMWLEGGRVKVRLRNSESVRAQRAIHDGEVHFVTVEVLDGRMSLYVAGQKQGDVEGRTVDVNAGDVVFVGGLPEKSSTSEFGGFFKGCIQDLRIGDTRLQFFGLDTSLTSYPLEQIENVIQGCPGDNACSQTKTLCKGQFYQIIMQRMFVSHSQRNPCLNGGICFSGWDDFTCTCPSSTAGRRCDEVKWCELSPCPTPAECKMVQQQQGYECYSNATFLNDSTVMTYRGNGHIFQNITSLSLNLRSRKRNAAILHAEKGSSFITVSVQDGLLFMELQSSSGEERVSTVSLSSRRSITDGEWHAVHLFMTAPWAPSSRWTLVLDDEIEEASLSKSQGSNLNFLREGVDIYLGGLAPFTGWSLAGCLGTVELGGIALPYFSSSEVNLPRLQIEQFIQKSPNSALPGCRGASVCEPSPCVNGGQCQDLFNTYNCTCAEGWAGRRCDVLIDTCASNPCLHGNCSINGLSYECTCEVGYTGVDCEEQVDVCKNHLCANGATCLHGPDKYACLCAENYTGPLCSIPKLPVSVCGDEVRNYTCFNGGNCTDRELSCDCPAGFIGHRCEQDVDECKSNPCLNGGYCRNLVNRFVCVCDMSFAGDVCQTDLTSEGLTSNLLLSISLASVLLLLVLILTSVGLVAALNRRATHGTYSPSRQEKDGSRVEMWSITQPPPMERLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCU6</accession>
    <name>A0A3B5LCU6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027896655.1">
        <property type="nucleotide sequence ID" value="XM_028040854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCU6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010116.1">
        <property type="protein sequence ID" value="ENSXCOP00000009998.1"/>
        <property type="gene ID" value="ENSXCOG00000007584.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114158934"/>
    <dbReference type="KEGG" id="xco:114158934"/>
    <dbReference type="GeneTree" id="ENSGT00950000182966"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004974">
        <property type="term" value="F:leukotriene receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000109">
        <property type="entry name" value="Leukotriene B4 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF946">
        <property type="entry name" value="LEUKOTRIENE B4 RECEPTOR 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="180"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="45"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="301" mass="34019" checksum="2BF34A53F0CD27C5" modified="2018-12-05" version="1">MEQLNATVVSTNFSSTEHPTLPSWKAGGLVSVVICIFCFLLGVPGNIAVILLKPNWQNLSSLSRTLMLNLSVSDLLCLVTLPIWAYSILYGWIFQLAACKLLAYLVYCSIHGSLLTITTLSVHRYLTVVRRQRCNQVQKRVLLVLLWLVALILSIPVLVVRQLKTYQQSAHCRSQFTSETQWVVLLLLESWFGFVCFSLVCCTYIRLHRKVKQTALFNHPQTTRLVISIIVSFFVLWIPYHTINLLAVAALSLQHVDLLNFCTDQQELAVSLTFLHSCLNPLLYAFSSPKMCTVCKQTAPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFL4</accession>
    <name>A0A3B5LFL4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Profilin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the profilin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027866586.1">
        <property type="nucleotide sequence ID" value="XM_028010785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFL4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010943"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011068.1">
        <property type="protein sequence ID" value="ENSXCOP00000010943.1"/>
        <property type="gene ID" value="ENSXCOG00000008264.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114140657"/>
    <dbReference type="KEGG" id="xco:114140657"/>
    <dbReference type="CTD" id="5216"/>
    <dbReference type="GeneTree" id="ENSGT00940000153664"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060027">
        <property type="term" value="P:convergent extension involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055113">
        <property type="term" value="P:epiboly involved in gastrulation with mouth forming second"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032233">
        <property type="term" value="P:positive regulation of actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00148">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048278">
        <property type="entry name" value="PFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005455">
        <property type="entry name" value="PFN_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036140">
        <property type="entry name" value="PFN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027310">
        <property type="entry name" value="Profilin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13936">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13936:SF17">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00235">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00392">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55770">
        <property type="entry name" value="Profilin (actin-binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00414">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="143" mass="15036" checksum="CC45101883F39972" modified="2018-12-05" version="1">MSWQSYIDTMKTADQSGMVPVAEAAICGIAAGQESVWVSTSGLQGITVDEIKKLGSKDRSSFAQNGVYIGGTRCRLIRDQMDMDPVFALDLKTAADADGNAFGVCVGKTKTAVVIAKGTKDASGGQISNKVFSVVEHLRKAGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFW1</accession>
    <name>A0A3B5LFW1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFW1"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008672.1">
        <property type="protein sequence ID" value="ENSXCOP00000008566.1"/>
        <property type="gene ID" value="ENSXCOG00000006558.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286950"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051051">
        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF5">
        <property type="entry name" value="E3 UBIQUITIN_ISG15 LIGASE TRIM25-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="15"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="90" mass="10278" checksum="5678168049F07167" modified="2018-12-05" version="1">MAQTGVQKDRERFSCSICLDLLKDPVTTACGHSYCMNCITDFWDGEEKKGTYSCPQCRETFRQRPVLKKKHPVSRVNRGPEEKQSQSCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LFW6</accession>
    <name>A0A3B5LFW6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LFW6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008677.1">
        <property type="protein sequence ID" value="ENSXCOP00000008571.1"/>
        <property type="gene ID" value="ENSXCOG00000006562.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000263473"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 20 MEMBER A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45784:SF3">
        <property type="entry name" value="C-TYPE LECTIN DOMAIN FAMILY 4 MEMBER K-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="34"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="153"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="2">
        <location>
            <begin position="257"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="365" mass="43278" checksum="23C884D8213D4066" modified="2018-12-05" version="1">MCLLDVTPSIHFLTPLIVWGWEGCWCPSPAVNRRELYQFHYINENKNWTEAQQYCREKHTDLATVTNMKDMKRLNNISAGDQSEAWIGLISKPEFTRTWFWSLPGVEFNESETNWHQGEPSDTGIQGFENCGIVYQDLTWGVQGCQWSEYFLCYDDMKTWLEAQSYCREKHTDLISGTKQLQDEEVKKLVDSEFKHLYIGLFRDTWRWSDGSSFSFRHWNNDFNYPQSNSGQCAMTVFNDGGRWKNVSCAERKPFICYHDKVILIKENKTWKDALTYCRDHHHDLVTITNMDDQRWIQEKVKNASTDFVWIGLRFNCSMNVWFWVCANWAWDEQNDCNMFGVMEAGGEYQWFQRNGSERFNFICS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHD6</accession>
    <name>A0A3B5LHD6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHD6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011675.1">
        <property type="protein sequence ID" value="ENSXCOP00000011543.1"/>
        <property type="gene ID" value="ENSXCOG00000008717.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163206"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016907">
        <property type="term" value="F:G protein-coupled acetylcholine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004969">
        <property type="term" value="F:histamine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007197">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007187">
        <property type="term" value="P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15296">
        <property type="entry name" value="7tmA_Histamine_H3R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000237">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041998">
        <property type="entry name" value="7tmA_HRH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003980">
        <property type="entry name" value="Histamine_H3_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF254">
        <property type="entry name" value="HISTAMINE H3 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01471">
        <property type="entry name" value="HISTAMINEH3R"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="181"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="289"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="38"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="220"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="232"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="340" mass="38517" checksum="7E35C1E8CDDA31AA" modified="2018-12-05" version="1">VQRFPPDRRLIAGIVLPGYLVVIFSVLMVTLVIVVVAGNALVIMAFIVDKTLRNQSNYFFLNLAISDFLVGAFCIPVYIPYNLTGRWMLGKGLCKVWLVMDYLLCSASVFNIVLISYDRFLSVTRAVKYRAQRNITRQAVLKMVAVWLLAFLLYGPAIIFWEAIVGQSVVPAHECYAEFYFTWYFLLSASTFEFFTPFVSVAFFNLSIYLNIHRRNKSGNGGAEDEDDDILSPSSSGCPDTTPGSRLSRDKKIAKSLAIIVCIFGICWAPYTLLMIIRAACGGECVAHYWYEITFWLLWLNSAINPFLYPLCHSSFRRAFSKILCPKRQSVQPQIEVQSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHQ1</accession>
    <name>A0A3B5LHQ1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">V-rel avian reticuloendotheliosis viral oncogene homolog A</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027894127.1">
        <property type="nucleotide sequence ID" value="XM_028038326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHQ1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007384"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007479.1">
        <property type="protein sequence ID" value="ENSXCOP00000007384.1"/>
        <property type="gene ID" value="ENSXCOG00000005689.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114157395"/>
    <dbReference type="CTD" id="5970"/>
    <dbReference type="GeneTree" id="ENSGT00940000159867"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009948">
        <property type="term" value="P:anterior/posterior axis specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007249">
        <property type="term" value="P:canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007498">
        <property type="term" value="P:mesoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038061">
        <property type="term" value="P:non-canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034097">
        <property type="term" value="P:response to cytokine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01177">
        <property type="entry name" value="IPT_NFkappaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.340:FF:000003">
        <property type="entry name" value="NFkB p65 transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000046">
        <property type="entry name" value="Nuclear factor NF-kappa-B p105 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033926">
        <property type="entry name" value="IPT_NFkappaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000451">
        <property type="entry name" value="NFkB/Dor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030492">
        <property type="entry name" value="RHD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032397">
        <property type="entry name" value="RHD_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169">
        <property type="entry name" value="NUCLEAR FACTOR NF-KAPPA-B PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24169:SF1">
        <property type="entry name" value="TRANSCRIPTION FACTOR P65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00057">
        <property type="entry name" value="NFKBTNSCPFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01204">
        <property type="entry name" value="REL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RHD" evidence="3">
        <location>
            <begin position="17"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="537"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="538"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="628" mass="69256" checksum="79C54F3AF91D1DE5" modified="2018-12-05" version="1">MDGVYGWGMTPINPVQPATPYIEIIEQPKQRGMRFRYKCEGRSAGSIPGEKSNDTTKTYPAIKVHNYSGPLRVRISLVTKNPPHKPHPHELVGKDCKHGYYEADLQERRIHSFQNLGIQCVKKKDVNEAITCRLQTSNNPFSIPEPKVWEEEFDLNSVRLCFQASITLPTGDMFPLPPVVSQPIFDNRAPNTAELKICRVNRNSGSCRGGDEIFLLCDKVQKEDIEVRFYQDSWEGKGTFSQADVHRQVAIVFRTPPYRDTNLSEPIKVKMQLRRPSDREVSEPMDFQFLPADPDEYRLSEKRKRTGDMFQNLKLGSMLSTVSVPQDRRHINLARRPVAAKLSSMNTQAAEVAPSASGLKPFSYNQPGQLFSVQPKVEASSSISTATTNQTWKIMESLKLGPTNPVQTFTMSSVSAPSSSTASTAANNEYSTVNLSDLHEFFPNISSPMAEESAAPQGSAAAPQTGSTFPLQESQFRVDPPLVDDEIPDFSNFTDAPVPGTLDSLNMDEFEDLLNPRLMSEGGSAASMLTSSQHGVLSSSAAASHNGASSQNLPDHVNNPGSTYMNYPNSIANLLRNEDMIILASSTNNNQQPAVLDDFDVLMSADEDRLISIFNSGSQAGFVSGHPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHU8</accession>
    <name>A0A3B5LHU8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Synapse</location>
            <location evidence="10">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHU8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007429"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007525.1">
        <property type="protein sequence ID" value="ENSXCOP00000007429.1"/>
        <property type="gene ID" value="ENSXCOG00000005730.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160483"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF16">
        <property type="entry name" value="RAS GUANYL-RELEASING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="5" id="KW-0771">Synaptosome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="13">
        <location>
            <begin position="3"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="12">
        <location>
            <begin position="151"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="249" mass="28791" checksum="164C43FCF5E39A93" modified="2018-12-05" version="1">MESILSEQAASVDELVEACIQAFDEKGTLKDASLVRMFLMMHPWYIPSTDMARKLVLQSREESCTEERRTRICHLVKYWISEFPAEFNLNPELEEQIKDFKDLLTTGGNERQSQLIDLDSVPSYKWKRQVTQRVPSVSKKRKMSLLFDHLDSSELAEHLTFLEYKSFCKILFQDYHSFVMHGCTVDNPILERFITLFNSVSQWIQLMVLSKPTAQQRAAVVSHFIRVAQVSPPPHLPGVSQHAVLCRSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIB0</accession>
    <name>A0A3B5LIB0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIB0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011828"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011964.1">
        <property type="protein sequence ID" value="ENSXCOP00000011828.1"/>
        <property type="gene ID" value="ENSXCOG00000008680.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163331"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004980">
        <property type="entry name" value="Ras homolog gene family, member Gd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="101" mass="11331" checksum="8A566E41706051D2" modified="2018-12-05" version="1">MQTIKCVVVGDGAVGKTCLLISYTTNAFPEEYIPTVFDNYSAQMSVDGRNVSLNLWDTAGQEEYDRLRTLSYPQTNVFIICFSIGSPSSHANVRHKWHPEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIN3</accession>
    <name>A0A3B5LIN3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cationic amino acid transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-arginine(in) = L-arginine(out)</text>
            <dbReference type="Rhea" id="RHEA:32143"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-lysine(in) = L-lysine(out)</text>
            <dbReference type="Rhea" id="RHEA:70935"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-ornithine(in) = L-ornithine(out)</text>
            <dbReference type="Rhea" id="RHEA:71199"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIN3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010737"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010861.1">
        <property type="protein sequence ID" value="ENSXCOP00000010737.1"/>
        <property type="gene ID" value="ENSXCOG00000008106.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164978"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015189">
        <property type="term" value="F:L-lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097638">
        <property type="term" value="P:L-arginine import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000024">
        <property type="entry name" value="High affinity cationic amino acid transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000009">
        <property type="entry name" value="Low affinity cationic amino acid transporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004755">
        <property type="entry name" value="Cat_AA_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00906">
        <property type="entry name" value="2A0303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF19">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
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            <begin position="528"/>
            <end position="578"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00004651"/>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    <sequence length="613" mass="66599" checksum="30D6CA9D2F25B44E" modified="2018-12-05" version="1">MSGAKMLQFLQSIFRRKPLEPEGEVSNFRRCLTTLDLVALGVGSTLGAGVYVLSGEVARTIAGPSIIISFLIAAVASIFAGLCYAEFGARVPKTGSAYLYSYVTVGEIWAFITGWNLLLSYVIGTASVARAWSGTFDDLIGHVIANSLGKGAMIRLPGLAPYPDFFAAGLIMLLSGILAFGVKESAIVNKVFTAINILVLLFIILSGFIKGDINNWFINEGTLQRAFEYGNYTFLNETSQCGTGGFFPFGFEGTLAGAATCFYGFVGFDCIATTGEEVQNPQKSIPLGIVASLLICFLAYFGVSAALTLMMPYYLLSVNSPLPMAFTYIGWGPAKYVVAIGSLCALSTSLLGSMFPMPRVLFAMARDGLLFRSLSKMSSRQSPVIATLVSGIVAAVMVLLFDLRMLVDIMSTGTLFAYTLVAICVLILRYQAELSEDSSLGKSEPFTVGGTLCPPSQGTPRTSRNVSILTIATVVICVILSILISKGMDSLRTLQWWSLLCVSVFGLILVVTVLLIWRQPQSSAKAAFMVPFVPWLPTFSTFVNVYLMVQLGSDTWIRYAVWMAVGLSIYFCYGVQHSVQKQRMENSQDRDKRGLMQTDTPDLKKVFCFLCRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJ74</accession>
    <name>A0A3B5LJ74_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Sodium/potassium-transporting ATPase subunit beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the X(+)/potassium ATPases subunit beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJ74"/>
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    <dbReference type="Ensembl" id="ENSXCOT00000009780.1">
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        <property type="gene ID" value="ENSXCOG00000007361.1"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:intracellular potassium ion homeostasis"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
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    <dbReference type="GO" id="GO:1990573">
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    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
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    </dbReference>
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        <property type="entry name" value="SODIUM/POTASSIUM-DEPENDENT ATPASE BETA SUBUNIT"/>
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        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT BETA-3"/>
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    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="11" id="KW-0915">Sodium</keyword>
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    <keyword evidence="6" id="KW-0740">Sodium/potassium transport</keyword>
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    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
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            <end position="74"/>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00037667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU362099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="285" mass="32908" checksum="54C70A794C52CF9F" modified="2018-12-05" version="1">MCGKQPILLCNLSKEDFVKESPSSWKDSIYNPRTGELLGRTAGSWALILLFYLVFYCFLAGMFALTMWVMLLTLDDYVPKYRDRIPSPGLAIRPNSLDISFNRSDPQKYSQYVRNLESFLQRYNDTEQEGNTDCSPGEYTLREEGEGLSQKACRFRRALLSFCSGLSDTNFGYHEGKPCVLLKMNRIIGLKPSGDPYINCTVKNPIQMHYFPKEGRFDKMYFPYYGKKAHENYVEPLVAVKLLFTKEDYNKELAVECKVNGSNLLNNDDRDKFLGRITFRIKVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJE1</accession>
    <name>A0A3B5LJE1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">trypsin</fullName>
            <ecNumber evidence="6">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="STRING" id="32473.ENSXCOP00000012203"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012349.1">
        <property type="protein sequence ID" value="ENSXCOP00000012203.1"/>
        <property type="gene ID" value="ENSXCOG00000009240.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234551"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000005">
        <property type="entry name" value="Serine protease 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF81">
        <property type="entry name" value="GRANZYME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017309934" description="trypsin" evidence="7">
        <location>
            <begin position="23"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="25"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="257" mass="28725" checksum="746F780296EA1A34" modified="2018-12-05" version="1">MFFNNKLPVLMVLLTLYDQGRTEKIIGGHVAEPHSRPYMVFIRRHLANNEESFCDGFLLNEDFVMTAAHCQANNYTIYLGVDNTKSLKNDEIQDRTVEKAFPHENYDKNAYIDDIMLLKLSSRAVVNNFVKPVALASQDDVTLPKSCIVSGWGQTRPDGVISDKLMEMNVTLIENSDCTKLKLYCSNGKARPGKGDSGGPLVCGQNTAYGVVAFMSQSPDGHSMVAYTKIPCYVDWITSTMKKALSKNVKFDNILLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLJ0</accession>
    <name>A0A3B5LLJ0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Collagen alpha-2(VI) chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Collagen VI acts as a cell-binding protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the type VI collagen family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLJ0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010406"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010529.1">
        <property type="protein sequence ID" value="ENSXCOP00000010406.1"/>
        <property type="gene ID" value="ENSXCOG00000007881.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155682"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01450">
        <property type="entry name" value="vWFA_subfamily_ECM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000027">
        <property type="entry name" value="collagen alpha-2(VI) chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000052">
        <property type="entry name" value="collagen alpha-2(VI) chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000026">
        <property type="entry name" value="Collagen, type VI, alpha 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052229">
        <property type="entry name" value="Collagen-VI/PIF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22588">
        <property type="entry name" value="VWFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22588:SF15">
        <property type="entry name" value="VWFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017374209" description="Collagen alpha-2(VI) chain" evidence="14">
        <location>
            <begin position="25"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="33"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="545"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="764"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="244"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="464"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="314"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="386"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="469"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="951" mass="101871" checksum="9F6B5463DDDDA333" modified="2018-12-05" version="1">KKTKKMLAFETVVLCLLLGRRASSQVADECPIHLYFTIDTSETIALQESPPGALVKSIRLFTEQFVQRLKNEEIRGGVRINWSIGGLHFSQTQEIFSPIQGKDGFLSNYLGGGTYIDCALRRMTEEMMRGSSSNVTPLRFAVVITDGHVTRSTCQGIKVRAEEARDKGIRVFAVASSNKIEETGLKEIANSPASVFRRDFLAVNLTQGRPIIHTPTMDRIIKTMVKLKHTRFPSLIMLDLVDQGAKGDNGEAGQKGERGRPGDPGIEGPIGQPGTKGEIGTQGKKGVAGIAGRNGTDGQKGKIGRIGAPGCKGDPGDKGPDGHPGDVGERGPPGSDGEKGDPGRPGRAGPPGQSGEGGPEGERGSNGSPGLPGTKGTPGLPGSPGKRGEEGRRGDYGPKGEPGLDGTKGDKGEMGPESLRGVPGESGSKGAKVERPILLDEGLPGQRYILINILQLSIPGFSYPGPRGDPVRDIGDPGKRGPPGSRGDCGQKGEPGNKGTPGESVRPRGDPGPEGDPGLSECDVMNYIRETCGCCDCEKRCGAVDIVFVIDSSESVGLSNFTLEKNFVINTINRLGSFATDPQSETGTRVGVVQYSHNGTFQAIHLNDSNINSLTAFKEAVKRLEWIAGGTWTPSALKFAYDHLIRDSRRAKANVTVVVITDGRFDPRDDDRLLPYLCNHPGVDVSAIGIGDMFEQVEENESLQSIACQKEGRVMGMRRFADLVAEEFIDKIETVLCPDPAIICPDLPCKSEPAVASCVQRPVDVIFMLDGSERMGLENHRRAKEFIENVARRLTLADGPTDDTRARLALLQFGSAGDHKEEFPLTHNLTVIADSLASVNYLDSSSALGNGIIYAVNNLVIRQSQRLSRRNAEVAFVFITDGITSTDQLDEGVDAMKRAEGVPTVIALGSDTDEEVLRKVSLGDSSAVFRGDDFSVLGKSAFFERFIRWIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LMR7</accession>
    <name>A0A3B5LMR7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Endophilin-A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Endophilin-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Endophilin-A2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Osteoclast-stimulating factor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">SH3 domain-containing GRB2-like protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Implicated in endocytosis. May recruit other proteins to membranes with high curvature.</text>
    </comment>
    <comment type="function">
        <text evidence="19">Induces bone resorption, acting probably through a signaling cascade which results in the secretion of factor(s) enhancing osteoclast formation and activity.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Required presynaptically at the neuromuscular junction. Implicated in synaptic vesicle endocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Early endosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the endophilin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LMR7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010806"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010931.1">
        <property type="protein sequence ID" value="ENSXCOP00000010806.1"/>
        <property type="gene ID" value="ENSXCOG00000008159.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154737"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016191">
        <property type="term" value="P:synaptic vesicle uncoating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07592">
        <property type="entry name" value="BAR_Endophilin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11803">
        <property type="entry name" value="SH3_Endophilin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000021">
        <property type="entry name" value="Endophilin-A2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035824">
        <property type="entry name" value="Endophilin_A_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF63">
        <property type="entry name" value="ENDOPHILIN-A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
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    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="14" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
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            <begin position="18"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="342" mass="38778" checksum="388419DFBBBAE80C" modified="2018-12-05" version="1">MSVAGFKKQFFKASQMMSEKVGGAEGTKLDEDFKELERKADVTSKAVVDVIGKTSEYLQPNPASRAKLTMLNTVSKIRGQVNSPGYPQPEGLLGESMTKYGRDIGEDNSFGGALVDVGEAMKRLAEVKDSLDIDVKQNFIDPLQAVAEKDIKDIQHHLKKLEGRRLDYDYKKKRQGKIPDEEIRLSLEKFHESKDMAESSMQNLLETDVEQVSQLSAFVESLLQYHRQATEILEEVTERLKERVNEAQSRPKREFKPKPRQSFDYGDEEHSNGGYAAGPVREPSCKALYDFVPENDGELGFYEGEIITLVRQIDENWFEGRLRGQTGYFPNNYVEVIVPISH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LN41</accession>
    <name>A0A3B5LN41_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Muscarinic acetylcholine receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cell membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="15">Postsynaptic cell membrane</location>
            <topology evidence="15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily.</text>
    </comment>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:postsynaptic membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:G protein-coupled acetylcholine receptor activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004993">
        <property type="term" value="F:G protein-coupled serotonin receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007197">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003161">
        <property type="term" value="P:cardiac conduction system development"/>
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        <property type="project" value="Ensembl"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0045987">
        <property type="term" value="P:positive regulation of smooth muscle contraction"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:saliva secretion"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="entry name" value="Musac_Ach_M3_rcpt"/>
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        <property type="entry name" value="Musac_Ach_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247">
        <property type="entry name" value="5-HYDROXYTRYPTAMINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24247:SF183">
        <property type="entry name" value="MUSCARINIC ACETYLCHOLINE RECEPTOR M3"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00243">
        <property type="entry name" value="MUSCARINICR"/>
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        <property type="entry name" value="MUSCRINICM3R"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 15" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="10 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0770">Synapse</keyword>
    <keyword evidence="12 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="82"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="162"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="443"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="481"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="17">
        <location>
            <begin position="62"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="300"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="309"/>
            <end position="326"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
    <sequence length="544" mass="61544" checksum="956C050B2BB471AD" modified="2018-12-05" version="1">YLHPNTFSGAYSQSSAHHKNLNFTPQPENQTTALDPLGGHPVWQVVLIVLLTGSLSLVTIVGNILVVVSFKVNRQLKTVNNYFLLSLAVADLIIGVISMNLYTVYLIMGYWALGTWACDLWLAIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTKRAGIMIGLAWLVSLVLWAPAILLWQYFVGKRMVPHNQCYIQFLTEPITTFCTAMAAFYLPVTIMSILYWRIYKETQNRSKELAGLQGSEGERARFVHQTGSSRSCGSYELNRMPKKKSTCQELVGRFHCWPGVRSWRPGSIRHGDGDPDQSSSDSWNNNDAAASLDHSGSSEDEDCGGREMISQSHAIFSIVLSLPGIKAAVNSQLTSCEDLDVASEKDPLRGDKNSYHQRFSSRKTQSMSTIQSSSVPLSFKEAAMAKRFATRTRTQITKRKRMSLVKEKKAAQTLSAILLAFIITWTPYNIMVLITTFCKVCIPQTLWAVGYWLCYVNSTVNPMCYALCNKTFRTTFKMILLCRWDQKKRRKLQFQQRQSVVFHRGIPREST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNA0</accession>
    <name>A0A3B5LNA0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NADPH oxidase organizer 1a</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016176">
        <property type="term" value="F:superoxide-generating NADPH oxidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042554">
        <property type="term" value="P:superoxide anion generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000233">
        <property type="entry name" value="NADPH oxidase organizer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051228">
        <property type="entry name" value="NADPH_Oxidase/PX-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706:SF10">
        <property type="entry name" value="NADPH OXIDASE ORGANIZER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15706">
        <property type="entry name" value="SH3 MULTIPLE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PX" evidence="5">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="152"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="221"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="294"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="387"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="429"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="429"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="460"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="477"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="501" mass="56563" checksum="79BC546566A071CC" modified="2018-12-05" version="1">MASERFPISIRLTGVLHKEKNKMYITSVLWSDQNEIVVYRSFEDFKEMHKQLKKKFPSGSKTKKSDRILPKFQSKGPNKSLVCLKYLQKYCNELLSCDQHVSQSADLAKFLHPNEKELKPEFTKNSIIIIPSEEEIRSYGRQDSSGNVTQPFVTETYRCIAPYETKDTKNKPFKVAVNEMLDVLIKDKAGWWLVENEEKRMAWFPAPYLENLEDEEDEEEVHGMFYIVVKSYNSNIVDEVSVNIGVVVEVLRTPDNGWWLIRHKGKMGYVPSLNLQPYINPQVQMTSQYNVQNSSSLLPHSSTLHHSRSYGNLSQHPNPRPISPSLTPTESIHRSQSLNQLSTQPAVSLPAPANNHPTSPTSPVQAPLPEILVQSDEDQEQNGRILRAESVDSFESDSFSDDALSMSTGSSLNLSYSANDQQLRFCRTPQPTVSNSLSPTSNPEGTLLPSVSDPNLYKGPQPPRVPPRPRAQEILTRCSSITRKNATKSNLSPTSTEVTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPF2</accession>
    <name>A0A3B5LPF2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the lid subcomplex of the 19S proteasome regulatory particle complex (also named PA700 complex). The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S9 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPF2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013978"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014151.1">
        <property type="protein sequence ID" value="ENSXCOP00000013978.1"/>
        <property type="gene ID" value="ENSXCOG00000010578.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063301"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 11"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050871">
        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040780">
        <property type="entry name" value="Rpn6_C_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040773">
        <property type="entry name" value="Rpn6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18503">
        <property type="entry name" value="RPN6_C_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18055">
        <property type="entry name" value="RPN6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PAM"/>
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    </dbReference>
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        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="321"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00062507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="420" mass="47189" checksum="B97E96C4FDF3C85D" modified="2018-12-05" version="1">MAAAAVAEFQRARSLIGTDRNASIDILHSIVKRDIQDSDEEAVRVKEQSILELGGLLAKTGQAAELGGLLKYVRPFLNSISKAKAARLVRSLLDLFLDMEAATGQEVELCLECIEWAKAEKRTFLRQALEARLISLYFDTKCYQEALQLGSQLLQELKKMDDKALLVEVQLLESKTYHALSNLPKARAALTSARTTANAIYCPPKLQAALDMQSGIIHAAEEKDWKTAYSYFYEAFEGYDSIDSPRAITALKYMLLCKIMLNAPEDVQALISGKLALRYAGRQTDSLKCVAQASKNRSLADFEQALTEYKAELRNDPIISTHLTKLYDNLLEQNLIRVIEPFSRVQVDKWLLCVLQRDVERKLSQMILDQKFHGILDQGEGVLIVFDEPVVDKTYEAALETIQNMSKVVDSLYNKAKKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQZ5</accession>
    <name>A0A3B5LQZ5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transmembrane serine protease 5</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQZ5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013467"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013633.1">
        <property type="protein sequence ID" value="ENSXCOP00000013467.1"/>
        <property type="gene ID" value="ENSXCOG00000010199.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159163"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF27">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15494">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="SRCR" evidence="9">
        <location>
            <begin position="68"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="183"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="432" mass="47856" checksum="F6869D2B7A825FFF" modified="2018-12-05" version="1">LIPLLTVSAAHRLVRLLAAVCAVGLLGGLLVGVWFLGDLAGVLVYFQNLIALCTYSLLLTESLSPEGILTQSGSVFYRISPENSLLEIQLGKLHTWLPVCSERWNSSLGTLVCRQLGYLRLTRHKGVNLTDVGPNYTDGFIQITLKQKSSLENMWHFRESCITGKVIALQCFECGARAKLPRIVGGVEAPLGRWPWQVSLYYSNRHTCGGSIITSQWIVTAAHCVHNYRLPQVSSWVVYAGIVTRSSAKIAQHAGYVVEKIIYNENYNHRSHDSDIALMKLQRPLNFSDTIRPICLPPYDYDLPGGTQCWISGWGYTRPDVHSPDTLKEAPVPIISTKKCNSSCMYNGDITPRMLCAGYTEGKVDACQGDSGGPLVCQDKNLWRLAGVVSWGTGCAEPNHPGVYSKVAEFLDWIYDMIEVDTFIEELVAQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LR05</accession>
    <name>A0A3B5LR05_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LR05"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014523"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014708.1">
        <property type="protein sequence ID" value="ENSXCOP00000014523.1"/>
        <property type="gene ID" value="ENSXCOG00000011012.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:magnesium ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046085">
        <property type="term" value="P:adenosine metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010394">
        <property type="entry name" value="5-nucleotidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31367">
        <property type="entry name" value="CYTOSOLIC 5'-NUCLEOTIDASE 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31367:SF2">
        <property type="entry name" value="CYTOSOLIC 5'-NUCLEOTIDASE 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06189">
        <property type="entry name" value="5-nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="372" mass="41861" checksum="3B8ED5C95DFA6EA3" modified="2018-12-05" version="1">KVFSQLPLTVFLSHPLCLQPPKGMNETEALREELLFKMTCVKPATLQPKPENSITIAVSSRVLFNMDKEQQIFEQQGMEEYIKYQVAHETEPFSPGPAFSFVKALEAVNTQLRDLYPESEELFDVVLITNNHAYVGLRLINTINHHQLFIERFCMTGGNSPIGYLKAYNTNLYLSSDPVKVHEALEQGIAAATMFTPEKMTEVSETQLRVAFDGDAVLFSDESEQIYKAHGLDKFFEHEKAHENKPLDHGPLKGFLEVLGKLQKKFYAKGQRMDCPIRTYLVTARSAASSGARALKTLRSWGLEIDEALFLAGAPKGPILEKIRPHIFFDDQMFHVEGAAEMGTVACHVPYGIAQRVPERKGLKDKEISSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LR75</accession>
    <name>A0A3B5LR75_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zgc:174906</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LR75"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011906"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012042.1">
        <property type="protein sequence ID" value="ENSXCOP00000011906.1"/>
        <property type="gene ID" value="ENSXCOG00000009011.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00540000073797"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="P:regulation of apoptotic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01671">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01670">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
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        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CARD" evidence="2">
        <location>
            <begin position="22"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Death" evidence="1">
        <location>
            <begin position="123"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="210" mass="23802" checksum="0CADF33CC81B22B1" modified="2018-12-05" version="1">MSSIIYPLDRSAMAEEATNVVHLLKGQKAALIEILRADADFVVQSAHSLYLVSDQGYEKIKSCRVPSEKVQELLDHVIMSGPKAAQGMLDLLKEKRMQETFPMLDFVKDLRVKTPLSGCVMVREKQLMIVAKGLGSSWREIGRLALGITNVTLEQIEEEKRTYTERVFAMLYYWRNQKREEATAAYLHSLLSQKVLAVPPESIECLLETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRP9</accession>
    <name>A0A3B5LRP9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UBA domain containing 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRP9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013480"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013646.1">
        <property type="protein sequence ID" value="ENSXCOP00000013480.1"/>
        <property type="gene ID" value="ENSXCOG00000009960.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182999"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14305">
        <property type="entry name" value="UBA_UBAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041928">
        <property type="entry name" value="UBA_UBAC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066:SF21">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="2">
        <location>
            <begin position="229"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="271" mass="31014" checksum="55195B2136E11924" modified="2018-12-05" version="1">SETFKYDKTENLSSFLLGTWFLSALVDFLLAQAFQFLFEYEVEELPAGLLAPVFSLFVPFYQSIPRMPVTQVLGHIHITNKTLVYIVGLQLLTSSPFMWLVALSGLISGGLYHSNALWLQKVFFVPVWVSSVGKYILEPLFSSSQPNDETPLGMGATLDIQRQQRMDLLDQQLLLAQYNEARRNVRRQPQVDSHTHTHRSRSRHTRLCLYVYHLYSSAIKLYSCFCSLNLENPFVNVARLVEMGFSRIDALEALRASNNDINMATNFLLQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSP9</accession>
    <name>A0A3B5LSP9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Estrogen-related receptor beta</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSP9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013840"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014009.1">
        <property type="protein sequence ID" value="ENSXCOP00000013840.1"/>
        <property type="gene ID" value="ENSXCOG00000009881.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153433"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042562">
        <property type="term" value="F:hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033993">
        <property type="term" value="P:response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06946">
        <property type="entry name" value="NR_LBD_ERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000009">
        <property type="entry name" value="estrogen-related receptor gamma isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050200">
        <property type="entry name" value="Nuclear_hormone_rcpt_NR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48092">
        <property type="entry name" value="KNIRPS-RELATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="NR LBD" evidence="9">
        <location>
            <begin position="76"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="301" mass="34629" checksum="75E41D718D049AD9" modified="2018-12-05" version="1">FQVKFIKGLTNLRVKTSKYTLFIWCLSVFSFPGVRLDRVRGGRQKYKRRLDTENNPYLGLTLPPPTKKPREYYSITNPSTLSHLLVAEPEKIYAMPDPTMPESDIKALTTLCDLADRELVVIIGWAKHIPGFSTLSLGDQMSLLQSAWMEILILSIVFRSLPYEDELVYAEDYIMDEEHSRLTGLLDLYVSILQLVRKYKKLKVEKEEFVTLKAIALANSDSMHIEDMEAVQKLQDALHEALQDFESSQHQEDPRRAGKLLMTLPLLRQTATKAVQHFYSIKVQGKVPMHKLFLEMLEAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LST8</accession>
    <name>A0A3B5LST8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">FIP-RBD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cleavage furrow</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Recycling endosome membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LST8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012451"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012602.1">
        <property type="protein sequence ID" value="ENSXCOP00000012451.1"/>
        <property type="gene ID" value="ENSXCOG00000009407.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000033742"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.2440:FF:000001">
        <property type="entry name" value="RAB11 family interacting protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037245">
        <property type="entry name" value="FIP-RBD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019018">
        <property type="entry name" value="Rab-bd_FIP-RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057316">
        <property type="entry name" value="Rab11-FIP3/4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051977">
        <property type="entry name" value="Rab11-interacting_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15726:SF5">
        <property type="entry name" value="RAB11 FAMILY-INTERACTING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15726">
        <property type="entry name" value="RAB11-FAMILY INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25450">
        <property type="entry name" value="Rab11-FIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09457">
        <property type="entry name" value="RBD-FIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144270">
        <property type="entry name" value="Eferin C-derminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51511">
        <property type="entry name" value="FIP_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FIP-RBD" evidence="13">
        <location>
            <begin position="362"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="157"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="425" mass="49279" checksum="E6F6CEFE0984DE41" modified="2018-12-05" version="1">MIFKSDNILFAPHFHGSDSHPCVCVCVCVCVCVCMFQIMFMNAGHTPQKMQQLYNSDLLDIYCSQCCKKVNLLNDLEARLRNLKANSPNKKISSTAFGRQLFQANLGVFGSSQGSSTEDLFTDSIDSCDLDITDKVSYLEKKVTELESDSLANSDLKSKLKHENTHLVHRVHELEEQVKDAETKAAETLGEEMKRHREAYSKMERDRNTEIDLLCCRVQQLEEENGDMSVNVSRLKSQSEKLDQEKQRMTDKLEDTSLRLKDEVDLYRKIMDKLWHNRHAFQKEKEAMQELIDDLRRELEHLQLFKLEMEHPGKGKGLSEYNAKTRESEMEHEVRRLKQENFKLRDQNDDLNAQILSLSLYEAKNLFSCHTKAQCLAAEIDNASRDELVDALKEQEEINLRLRQYMDKIILAILDHNPSILEIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUG7</accession>
    <name>A0A3B5LUG7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HTH psq-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUG7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014680.1">
        <property type="protein sequence ID" value="ENSXCOP00000014495.1"/>
        <property type="gene ID" value="ENSXCOG00000010994.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="72" mass="8072" checksum="BB37955B8EAE10DB" modified="2018-12-05" version="1">FRNKMLPLIVAAVESGEGFLEVSKKFETSSSTVRNIICKLKTFKTIPIMPNRIVPCNMTMAQNITVNPQRTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVE4</accession>
    <name>A0A3B5LVE4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transcription factor 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TCF12</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVE4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015122"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015311.1">
        <property type="protein sequence ID" value="ENSXCOP00000015122.1"/>
        <property type="gene ID" value="ENSXCOG00000011453.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155047"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18945">
        <property type="entry name" value="bHLH_E-protein_TCF4_E2-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000001">
        <property type="entry name" value="Putative transcription factor 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051098">
        <property type="entry name" value="NeuroDiff_E-box_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11793">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11793:SF11">
        <property type="entry name" value="TRANSCRIPTION FACTOR 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="8">
        <location>
            <begin position="554"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="26"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="119"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="325"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="498"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="536"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="625"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="328"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="545"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="632"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="650"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="659" mass="70360" checksum="98E0E4D096C37BB2" modified="2018-12-05" version="1">MNPQQRIAALGTDKELSDLLDFSAMFSPPVGGGKNRPTTLGSSQFSASGMDERTNQASWAPGGESSPSYESSRGFADSPHYGDHMSDSRLVSHEELSPTPFMNSNIIGKTERASFLGYGRDPGASGSQASLRPDAALTSPNSVTTSGKSTAPFYSFNAANPRRRSLQDAPLIGYCCCKSDFNRDSPSYSSPKPSSSMFASTFFDGTHSSSDPWNSSSGISQPGYGGMLAGSSSHMAQTGNYSSLHSHDRLNYPAHSVSPDINASLPPMSSFHRSNTSTSPFVTAAHTSSVNSIDGVMVAANRGTTSGSSQTGDALGKALASIYSPDHTSSSFPSNPSTPVGSPSPLTATAGAASAGTVWPRARGQTPPSPNYENSLHSLSRMEDRLDRLDDAILVLRNHAVGSTASLPSDIHTLLGQAQNGPITTIGPNFPASALVPSRTAAMWAENGALRNSTFHGGLPSTGSTSSTELNHQLETFRGLASALSGQIPAALELKIEKQEKDDMHDSLSDNKSDDESDRRDMKTFSLLSLSLSPRSITEDEDLNPEQKAERERERRMANNARERLRVRDINEAFKELGRMCQLHLKSEKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVSGSSSDAQQGHTGVHPGLTDTSNPLGHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVR2</accession>
    <name>A0A3B5LVR2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVR2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012045.1">
        <property type="protein sequence ID" value="ENSXCOP00000011909.1"/>
        <property type="gene ID" value="ENSXCOG00000009014.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000176274"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="12"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="107" mass="12470" checksum="46F3A2B50B751473" modified="2018-12-05" version="1">MRFILFYYSFCSVHNARLIKEFFQENNITLLDHPACSPDLNPIENIWGWMAREVYKNGHQFQTVDALREAIFNTWSNVPTSLLETLASSMPKRLFEVINKNGGATHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVT9</accession>
    <name>A0A3B5LVT9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transmembrane protein 236</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TMEM236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVT9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015267"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015458.1">
        <property type="protein sequence ID" value="ENSXCOP00000015267.1"/>
        <property type="gene ID" value="ENSXCOG00000011564.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015525"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020394">
        <property type="entry name" value="Uncharacterised_FAM23-like_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31453">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31453:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="296" mass="33127" checksum="B0E867B807C7902E" modified="2018-12-05" version="1">MGSGSTLKFALCEVLQFAGLCVPLFIVMQRFAIIVAKVKASAQPPSDTISDVLTVCSHQLGSECSPDWNKTGWCETKQVQINNNMKHDTFTEIPVSLVFFSLICIDVVERIRNCRLTGQGERGIHPEINGAIPGINGTPGRPFIISSPELSSPSMSGPTYHLSPYTYTGPLRFLSISDARADVFVDSFMFWMDTVEMVRVAGHPLVYYSGWVFPIYIFSYVSCLRVVVMPNTPLLSSLGVALQDLPFFFVRVSLIALFGFVTPVLYLMKNLLVCLAFVYFNFMTKLRVFNTERMFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LW13</accession>
    <name>A0A3B5LW13_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Choline transporter-like protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Choline transporter.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>choline(out) + n H(+)(in) = choline(in) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:75463"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ethanolamine(out) + n H(+)(in) = ethanolamine(in) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:75467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Cell membrane</location>
            <topology evidence="2 16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LW13"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016359.1">
        <property type="protein sequence ID" value="ENSXCOP00000016153.1"/>
        <property type="gene ID" value="ENSXCOG00000012207.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158178"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385:SF34">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="331"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="426"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="474"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00036560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU368066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="515" mass="58153" checksum="EF2AF9A4A9169572" modified="2018-12-05" version="1">MMSLMLFSRGCTDILCCLLFILFLFGYFAVGILAWSQGDPRKVIYPTDSRGQFCGQAGTKPFLFYFNILKCASPLVLLELQCPTPQICVEKCPDRHLNLVKAKLGSNEDREYYSRYCKEGTDFTILRDALCPAMLLPSKAFTRRCLPALGKLSGGVVVVGNETSFFDDNNNRVNATDLLEASKKSNIVLEARQVAMRIFEDYTQSWHWILLGLVIAMVISLIFIVLLRFLAAVMVWVIIILVILVIGYGIFHCYMEYAALKGEPGADVTIRDLGLQTDFSVYLQIQQTWLAFMIILAIVEFIIILLLIFLRKRILIAIALIKEASRAVGHIMSAMFYPLLTFALLAVVIAYWAVTAVFLSTSNEQIYKVYNKSDCPFSMDTCDPKTFNTSNVSAQCPDAECLFAFYGGETLYHRYLILLQFYNVFLFFWCANFVTALGQVTLSGAFASYYWAFKKPDDIPAYPIFSSLGRALRLYHTGSLAFGSLILSLVQVIRVLLEYLDHKLKGWKNASLNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWZ6</accession>
    <name>A0A3B5LWZ6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inositol 1,4,5-trisphosphate receptor-interacting protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Enhances Ca(2+)-mediated inhibition of inositol 1,4,5-triphosphate receptor (ITPR) Ca(2+) release.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus outer membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWZ6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016448"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016655.1">
        <property type="protein sequence ID" value="ENSXCOP00000016448.1"/>
        <property type="gene ID" value="ENSXCOG00000012411.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244827"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026250">
        <property type="entry name" value="ITPRIP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024810">
        <property type="entry name" value="MAB21L/cGLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656">
        <property type="entry name" value="CELL FATE DETERMINING PROTEIN MAB21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656:SF8">
        <property type="entry name" value="INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02107">
        <property type="entry name" value="INOS145TPRIP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01265">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="506" mass="58462" checksum="4F1086AA4294FF13" modified="2018-12-05" version="1">MQDTLVRVFVVAAGLLLCPRDNPGVEEWDDAVDEDLQKHEQMLLEGEEKLMENETSEKGIAKWERDYLWYIWNTFSIISIMRFFRKFLKRNFKTDLEETRISPRRSPEVSLPDVETLQSFYAQCVQASAEKMLREKGFLEGFANDLLEAMRTVCENNGSMLIEDFQMVKERDIIVHFAPCKPYSVRCLLGNNPVSDPLLYMQVCGQIKLLKEEKVPNGCPCQSADADDMVCLVHSESDKAKTKVADAFNGPLCSKNTPFLSKSKITRWFQNTVKQAWAQISHKYEFELSIRYVETPGALVVRFKSGKKINFSLKPVVKFNTEAHFCITPWSSRDVDTFWVLSLSVYEDRLLEHLSKRLPVNSCRDQILEIAFFLHRRQEALTGKTALKDIHFKMALMHLLLAKKTSQWQPNFATCRLQDLLSFVEESLKRKHLTHILVGNPLAEVTGLPKEICRAKPVNLFHLFVEDDCAYKNAVMHFQEMFQNAHMLIHEYVVQPRAVLNICYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYB9</accession>
    <name>A0A3B5LYB9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tubulin-specific chaperone D</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Beta-tubulin cofactor D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Tubulin-folding cofactor D</fullName>
        </alternativeName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Tubulin-folding protein implicated in the first step of the tubulin folding pathway and required for tubulin complex assembly. Involved in the regulation of microtubule polymerization or depolymerization, it modulates microtubule dynamics by capturing GTP-bound beta-tubulin (TUBB). Its ability to interact with beta tubulin is regulated via its interaction with ARL2. Acts as a GTPase-activating protein (GAP) for ARL2. Induces microtubule disruption in absence of ARL2. Increases degradation of beta tubulin, when overexpressed in polarized cells. Promotes epithelial cell detachment, a process antagonized by ARL2. Induces tight adherens and tight junctions disassembly at the lateral cell membrane. Required for correct assembly and maintenance of the mitotic spindle, and proper progression of mitosis. Involved in neuron morphogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. Interacts with PPP2CB. Part of a supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state. Interacts with ARL2; interaction is enhanced with the GDP-bound form of ARL2. Does not interact with ARL3, ARL4A and ARL4D. Interacts with beta tubulin. Interacts with TBCE.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell junction</location>
            <location evidence="4">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lateral cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TBCD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LYB9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014341"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014525.1">
        <property type="protein sequence ID" value="ENSXCOP00000014341.1"/>
        <property type="gene ID" value="ENSXCOG00000010850.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017103"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016328">
        <property type="term" value="C:lateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048487">
        <property type="term" value="F:beta-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034333">
        <property type="term" value="P:adherens junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070830">
        <property type="term" value="P:bicellular tight junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031115">
        <property type="term" value="P:negative regulation of microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000268">
        <property type="entry name" value="tubulin-specific chaperone D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058033">
        <property type="entry name" value="ARM_TBCD_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033162">
        <property type="entry name" value="TBCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022577">
        <property type="entry name" value="TBCD_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12658">
        <property type="entry name" value="BETA-TUBULIN COFACTOR D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12658:SF0">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23579">
        <property type="entry name" value="ARM_TBCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25767">
        <property type="entry name" value="ARM_TBCD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12612">
        <property type="entry name" value="TFCD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0796">Tight junction</keyword>
    <feature type="domain" description="Tubulin-folding cofactor D ARM repeats" evidence="20">
        <location>
            <begin position="302"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin-folding cofactor D C-terminal" evidence="19">
        <location>
            <begin position="895"/>
            <end position="1080"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00064145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00077974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00079875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF12612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF25767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1184" mass="132760" checksum="09964DEC7D9788BD" modified="2018-12-05" version="1">MDLSEAETGCPEGGEEEADAIIKTCVLGGFSESSETRTLIGSLPEIHEDDRAGEFAVQKFRVIMDRYQDQPHLLDPHLEWVTNMLLSFIRNENSPPSLFHLSFQFLYGICKVRGYKVFMQLLPHEVSDVHPVLEMISRQDPKDLKTWETRYMLLLWLSMTCLIPFDLSRLDGHLESEGVRIKEPVMERILGNVALGICLPCRLVFILIKPLIFLLSDSTLPYRFMTRPDVKQKHLGCFLDWSLTSISQSSGDSMIEIRKLDGVLQSLATLFKHGKRDDFLQYAPIVLCNLEEKRLSESSEAMLRKIHIKLIQRLGLTFLKPRLAAWRYERGSRSLAANLSVSHTAAALTAAVTPETETREQEEDYDIPEEVETVIEHLLVGLKDKETVVRWSAAKGIGRVTGRLPKELADDVVGSVLDCFSFQETDNAWHGGCLALAELGRRGLLLPSRLTDVVPLIMKALTYDEKRGACSVGSNVRDAACYVCWSFSRAYDPKELQPFVTQIASALLITAVFDRNINCRRAASVKIFYAFLHQGTFPHGIDIVTAADYFAVGMINNCYLNISVFIAGFPEYTKSMVEHLISRKINHWDGAIRLLTTKALHNLTPQAPDYMATTVLPQLLTTAVGVDLHSRHGAILACGEITYALYKVALENNRTVMDLISPECVDALKNIHNLVSERGVLSNTCCLGFGGALMRPAICSLIEKLSLSKMPFKNDPIITAWQSLIDDTIKNLHLFSSGVKEDFIVSGRIYQYVQVYKAYLEGLKSPQLLTRCGSARALGCLPRFMIQSKLKQIFGGLEQMCTVSQKEGNFTEARRDSVRSVTGVCPHGSPDSVLCSENVVKVFGFLLCSINDYTTDSRGDIGAWVRKAAMTSLQEITLMVSSSAPEILSPHLVKSMMCCLAQQAAEQNDHYRAHAGNIFLHLLHNTEPVVPHIPHKEELLSIFPVETITSLNWNAPSQAFKYITQLLGLPEYQYHTLLGLSVSVGGITESTAHHSSQSLFAFLNGIQKDIDALTQFTQTLLRIFKNNLQNDRVSVPLLMMLSQMLANNVFEILTTQENHQVCVDLLGLCKELRKSRNVAKIRACIYVYCGLIQFEGDVRKKVLSQLLMLLCHAFPIIRKTTSGEMYEMLLTYNDVADPDVLEDVLNLLSDTQWESDLATVRERRNQLCDWLGVPRPRPITKVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZ97</accession>
    <name>A0A3B5LZ97_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA-3-methyladenine glycosylase</fullName>
            <ecNumber evidence="4">3.2.2.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">3-alkyladenine DNA glycosylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">3-methyladenine DNA glycosidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">ADPG</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">N-methylpurine-DNA glycosylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.</text>
            <dbReference type="EC" id="3.2.2.21"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Binds MBD1. Binds SSBP1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA glycosylase MPG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.21" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZ97"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014681"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014867.1">
        <property type="protein sequence ID" value="ENSXCOP00000014681.1"/>
        <property type="gene ID" value="ENSXCOG00000011147.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009825"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003905">
        <property type="term" value="F:alkylbase DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00540">
        <property type="entry name" value="AAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.300.10:FF:000001">
        <property type="entry name" value="Putative 3-methyladenine DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.300.10">
        <property type="entry name" value="Methylpurine-DNA glycosylase (MPG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00527">
        <property type="entry name" value="3MGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003180">
        <property type="entry name" value="MPG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036995">
        <property type="entry name" value="MPG_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00567">
        <property type="entry name" value="3mg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10429">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10429:SF0">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02245">
        <property type="entry name" value="Pur_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="FMT C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="294" mass="32864" checksum="392794F76DE2868A" modified="2018-12-05" version="1">MAGRKRKVQDLAVAAAAAHTESGLLLRRNQTELNCRASPAKLKSQPVLCESVQSLEPERSHYFNYNEPQRRLGQDFFNQPCISLAKAFLGKVLVRRRADGSELRGRIVETEAYLGGEDKASHSAGGKRTERNTAMFMKPGTIYVYPIYGIYLCMNVSSEGEGAAVLLRSLEPLLGQPAMRELRAARRKEGARQLKDKELCNGPSKLCQALNIPRCFDRRDLASDPEVWLEDDQNTSPPKPQDIVSAPRVGIESHGEWAKKPWRFYLRGHPCVSVVNKEAERLSLSGNETDNVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZV6</accession>
    <name>A0A3B5LZV6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Integrator complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the Integrator subunit 4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZV6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016525"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016735.1">
        <property type="protein sequence ID" value="ENSXCOP00000016525.1"/>
        <property type="gene ID" value="ENSXCOG00000012477.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010128"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016180">
        <property type="term" value="P:snRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000118">
        <property type="entry name" value="Integrator complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000134">
        <property type="entry name" value="Integrator complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20938">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20938:SF0">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Phosphatase 2A Regulatory Subunit A helical" evidence="8">
        <location>
            <begin position="239"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="476" mass="53028" checksum="8DBE08368BFBAAB2" modified="2018-12-05" version="1">MAAHLKKRVYEEFSKVVQIPLEEAPVKKLRLSKPSKSAALHIDLCKAANATDALQYLLEFARKPVEAESVEGVIQILLEHYYKESDNSVRLKIASLLGLLSKTNGFSPDCVVDDVINTLGNEKSHQVLAQLLDSLLAIGGQLPESLAVRRRLVQVACKLLSDTNFGVRNKCLQLLGFLGTVETPLTKDGEGPRVRDVQSIISTYFSDQDPRVRTAALKAMLQLHERGMKIHQIIYEQACRLLSDDYEQVRSAAVQMVWVLSQLYSESIVPIPSSNEEIRLVDDAFGKISHMVSDGSWMVRVQAAKTLGSMLQVSHHFLEQTLDKKLMSDLRRKRTAHERAKELFASGEFSSGRKWADDAPKEKLDTNTVNLIASGACGAFVHGLEDEMFEVRIAAVEALCQLARSSPSFAEKCLDFLVDMFNDEIEEVRLQSIHVLREISTHITLREDQLDTVLAVLEMFEVSWISPPNTGVAAGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0F3</accession>
    <name>A0A3B5M0F3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CUE domain containing 2</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CUEDC2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0F3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017583"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017806.1">
        <property type="protein sequence ID" value="ENSXCOP00000017583.1"/>
        <property type="gene ID" value="ENSXCOG00000013262.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014513"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd14367">
        <property type="entry name" value="CUE_CUED2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039805">
        <property type="entry name" value="CUE_CUED2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12493">
        <property type="entry name" value="CUE DOMAIN CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12493:SF0">
        <property type="entry name" value="CUE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="268" mass="30038" checksum="E831FD2573001E5B" modified="2018-12-05" version="1">MDLHKIIHSALHEFIQTYIPDADLSTLDDVLLSYITGVLEDLGSQQSVEENFDVEVFAEMLEAYIPGFAEIDSVKVCEMMFSLASKLATARTSGKCYQPEPGQEPPCLKPQTEGATAKLSASEWESQEQHLLEMFPKCSLSEARSALSIAKGDMEEAVRLIIEGDVQLSPTPINVSLSVNQGKSISSLADQKLKESILEKYMLVDSEEDNKTHRPVAPKDAPKKLVRYHGNQVVTTKGERYQLVKKNETDDMKKTYVNLKPARKYRFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0H9</accession>
    <name>A0A3B5M0H9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like modifier-activating enzyme 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">UFM1-activating enzyme</fullName>
        </alternativeName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ubiquitin-activating E1 family. UBA5 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0H9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015116"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015305.1">
        <property type="protein sequence ID" value="ENSXCOP00000015116.1"/>
        <property type="gene ID" value="ENSXCOG00000011451.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156177"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071566">
        <property type="term" value="F:UFM1 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071569">
        <property type="term" value="P:protein ufmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00757">
        <property type="entry name" value="ThiF_MoeB_HesA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000066">
        <property type="entry name" value="Putative ubiquitin-like modifier-activating enzyme 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF9">
        <property type="entry name" value="UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="9">
        <location>
            <begin position="52"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="324" mass="36419" checksum="A2A608520FB00546" modified="2018-12-05" version="1">MATVEELKLRVRELENELIKCKQRQCAAEDAHNRPRIDKMIGLLNNYLGFSVVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVELANMNRLFFQPHQAGLSKVEAAEHTLRNINPDVAFETHNYNITTLDNFTHFMDRISHGGLEEGKPVDLVLSCVDNFEARMAINTACNELGQIWMESGVSENAVSGHIQLIIPGETACFACAPPLVVAANIDEKTLKREGVCAASLPTTMGVVAGLLVQNVLKYLLKFGTVSYYLGYNAMQDFFPTMAMKANPQCNDRHCRRQQEEFKMKEAERPKVEAVQQEEEEIIHEDNEWGQQKLWFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M1B6</accession>
    <name>A0A3B5M1B6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Lysophosphatidic acid receptor 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LPAR4</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M1B6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013814"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013983.1">
        <property type="protein sequence ID" value="ENSXCOP00000013814.1"/>
        <property type="gene ID" value="ENSXCOG00000010458.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000240444"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070915">
        <property type="term" value="F:lysophosphatidic acid receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15155">
        <property type="entry name" value="7tmA_LPAR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000017">
        <property type="entry name" value="lysophosphatidic acid receptor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF41">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01067">
        <property type="entry name" value="P2Y5ORPHANR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0391">Immunity</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="137"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="306"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="352" mass="39324" checksum="4DEA4052203FD803" modified="2018-12-05" version="1">MASLVINETGVEDCGIDDSFKYNLYSTVYSVVFVSGLMTNCAALFVFCFRMKIRNETTMFMTNLAFSDLVFVFTLPFKVFYNVNRNWPFGDGLCKVSGTAFITNIYGSMLFLTCISVDRFLAIVYPFRSRSIRTRRNAALVCAAVWLTIVGGGISVTFFSTINSRHRATTCFEGFSKNTWKTYLSKITIFIEIVGFLFPLLANLVCSSLVLRTLRRPVSAGHGCDSKKRVLRMIVVHLTIFIICFVPYNFLLFLYALVRTQALANCTVERFARTLYPITLCLASLNCCLDPVVYYFTSESFKKSLTIGGKGSGSRPESVPRSDNEAQDTAVPRDTQSVSSNGKDATISDSQL</sequence>
</entry>
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    <accession>A0A3B5M1W1</accession>
    <name>A0A3B5M1W1_9TELE</name>
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            <fullName evidence="5">Ig-like domain-containing protein</fullName>
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        <name type="scientific">Xiphophorus couchianus</name>
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    </reference>
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    </evidence>
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    <sequence length="225" mass="24669" checksum="18C91A8E3FAE4AC4" modified="2018-12-05" version="1">MELLQLLLQLLLCVLLCVSAEVQHDSFRVIGCSDTDGLDMMTLDGEEELIPCHPSFSGLYEQSVGQQQLCKNNLKIIRTGLKDIPDPLGEERSTLLLYTANIVELGEKNILVCHVSGFYPAPVNVSWTKNGQKVTEGTSINVPFPNQDNTFTQISRLQFVPQLGDIYSCSVEHLALEEPLTRIWDVEVRVPQPGAGPAVFCGVGLIVGLLGVAVGTFFLIRGRES</sequence>
</entry>
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    <name>A0A3B5M2E1_9TELE</name>
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        <recommendedName>
            <fullName evidence="8">Trinucleotide repeat-containing gene 6C protein</fullName>
        </recommendedName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Ovalentaria</taxon>
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            <taxon>Xiphophorus</taxon>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
            </authorList>
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        <text evidence="1">Belongs to the GW182 family.</text>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1590" mass="166532" checksum="7338A99FEDB83232" modified="2018-12-05" version="1">MATGATGQQAHYNSLKPSSTMMTGPVSTSTLAASRGWGSDGKQDGMNGGRVAAPSNWGSPNFNLNLNPNANPSAWPVLGHEGGAGGVNGPSSSSLPPGIHGNGNVGNGNADNGGGSWVSMISGNDNDQQHLSTNKNLSFNMEPANLNTDGPNYTKQQQAQEPMSPIHGLTGWGGHSPTESSQLSGDTTGSSVWGGGENKMTESPKDSGWDSSPSGGLSAWGRQGSGGGSSGSGGWGDWGKSSGGGDISKGWDSVDAGNSGPGQDHQMSSWGKQSGTAPASEDSGDSSEGRSRCRDRSSSMEFAPLLPRQDLDPRVLSNSGWGQTPIRQHTVWEMEVANSDDGKSISSSDTMGCSSSNGGPSSTNGGAINPNIGPSQSAPPPQPIQTGSGWGDPPLSATKAPNGTACGWGDPLPTSGPKNEDCSSWGSDEKSSSWDDGMMKNKPSSWGESPKNSHSWGNSNVGSSGSTTGEWGAPDMKKNGSSSSMWEGEGSNGENSGWNESPRGGTRGGGSWGKPTPAVSNSNWGETPRASGPVQGGWGSAKPQERCVSIGSWGGPGSVKQSNPGWGNASKQEPGTEPTGWEEPSPPSIRRKMEIDDGTSTWGDPNAYSKTVNMWDRNNPNNNPGNSGPPPNKNGAVATISNNNNHSHHMHHHSHHGQPPSWGELPSVQPKPEPAWGEPAAPTSAVDNGTSAWGKPPGGVGGWGDGGHEPPGPYVRSNGHTGSAPCPKPMQDGWGNGEEMSMSTSQWDTEDGDVWNSPTSQDSSSSCNSWGNGPKRCPSKGKIGSKQDETWIMNRLIKQLTDMGFPRDPAEEALKSNNMNLDQAMTALLEKKTDLDKRGLGMSDYSNGMNKTLVCRPSALSKDPSDRNTFLDKDNVLSDDAPPSPFLPSPSLKLPLANGSLAGQGLGQGNPGLAMQNLNNRQIPNGMFGGNGAAQTRAMQQQPPQPPVPPLSSSQPSLRAQVPQFLSPQVQAQLLQFAAKNIGLNPALLTSPINPQQMTLLYQLQQLQMAYQRLQIQQQMMQAQRNVSGPIRQQEQQVARTITNMQQQIQQHQRQLYQALLMKQQQLPSHSSSSSSSAGLHPPGGPAGVPGSGKSTLDPFTGPHQAPGLADTLHTKEPPSSPNAYSTYPLSGLNPNMNVNGMDVGGLSLKEPPQPQSRLSQWTHTNSMDNLSANSSNLENNFNNKHGAISAASTLGPPGKHPQLEDSYSPYNLISNSESPSSPLVPPDNWGQGKSPNEKISNGANINWPPEFCPGVPWKGLQNIDPENDPNMTPGSVPSGPTINTNIHDVNRYLLRDRNGVSPLGNGSLPPTSSDWPISDLSSSFSLLSSDGESSGKLTEMKSTWSPGPIAQSQASLSHELWKVPQGPRSTTAPSRPPPGLTNTKPSSTWGGSSLGLAQEGTTWSTDSASRTSSWLVLRNLTPQIDGSTLRTLCMQHGPLITFHLNLTQGNAVVRYSSKDEAAKAQKSLHMCVLGNTTILAEFAGEEDVNRFFAQGQSLGATTTSWHANPGSNQNRMGGNSQSHSIGQWSSGGGGGGGGSKASGSDLLWGGVPQYSSLWGPPNGEDARVIGSPTPINTLLPGDLLSGESM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3E9</accession>
    <name>A0A3B5M3E9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fibronectin type-III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3E9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017987"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018211.1">
        <property type="protein sequence ID" value="ENSXCOP00000017987.1"/>
        <property type="gene ID" value="ENSXCOG00000013544.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155126"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050991">
        <property type="entry name" value="ECM_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708:SF7">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46708">
        <property type="entry name" value="TENASCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="66"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="157"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="245"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="338"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="430"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="2">
        <location>
            <begin position="519"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="609" mass="66812" checksum="2F297591B65155E1" modified="2025-10-08" version="2">MVTPLSSRGQLLLNQQRQLDRKADVSSAVVTELSPKTEYSLTLYAVYPNLISNSETITVKTTSLPKVSNFRVIEEGLFSLRLGWTSPLGKLNGFKIFIPRSNRPGLAYEQLLPGDISSHVIDGLEEDKKYTVNIYAVYPEGPSEPVSLVGKTLKLVAVKQFLVQNATTDTVQARWESVKGATGYRLTWASPDGHIENINLGENFNFYMIQGLHSGSEYIVTINPIFGDTEGPITTNNVKTLESSAVQTLKASAVSTSSAVVSWNSVPGATGYRLAWGPTPEFAGRDRPRQLALNSSTTEYKLKNVAHDTGYVLTLYVLFGSAVGPGISATFRTSPLGYVSNFKVTSYTSTSIAVEWSPIVGATEYKLSWYTDNGKPQSQYLDRSVLSHQIKNLIPQSTYTITILAVYGNLEGPDISLSQLTFSESEPIQPVRELKVVDIGVNSFTLSWKKTPGVSGYKIYWIPFLGGKEKSQVVPAASTSFTMDNLQESSAYKIQVSPMIGGREGSPVLVTARTLDLPKVEGFAALNTTESSAVLNWTRVAGVSGYLLSWRHISVLDTKTETLGPAFSSYKISDLQYGRTYIFSIRPLYGEVEGPISTIYQRICKNLHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M421</accession>
    <name>A0A3B5M421_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DEAD/DEAH-box helicase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M421"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018207"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018438.1">
        <property type="protein sequence ID" value="ENSXCOP00000018207.1"/>
        <property type="gene ID" value="ENSXCOG00000013713.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158694"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF6">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD/DEAH-box helicase" evidence="5">
        <location>
            <begin position="58"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="180" mass="20753" checksum="FA3B85ED455F4A2E" modified="2018-12-05" version="1">TTLGTRLSFRRIHDSTSETQTADLKPTLMYNSKFWGLPKPVLERYQKHGVTQMFEWQAQCLTVGQVLHGGNLVYSAPTSAGKTLVSELLILKRVLETKRKALFILPFVSVAKEKMHYLQFWLPPPPFHLESIFRLAGSSFELQLLTDACWTRSHTIRWPDEQGEKEWTPKVWQQGEVHCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M511</accession>
    <name>A0A3B5M511_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Vesicular inhibitory amino acid transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Solute carrier family 32 member 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Vesicular GABA transporter</fullName>
        </alternativeName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Antiporter that exchanges vesicular protons for cytosolic 4-aminobutanoate or to a lesser extend glycine, thus allowing their secretion from nerve terminals. The transport is equally dependent on the chemical and electrical components of the proton gradient. May also transport beta-alanine. Acidification of GABAergic synaptic vesicles is a prerequisite for 4-aminobutanoate uptake.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>4-aminobutanoate(out) + n H(+)(in) = 4-aminobutanoate(in) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:70979"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>beta-alanine(out) + n H(+)(in) = beta-alanine(in) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:70987"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>glycine(out) + n H(+)(in) = glycine(in) + n H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:70983"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Presynapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027859491.1">
        <property type="nucleotide sequence ID" value="XM_028003690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M511"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018460"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018692.1">
        <property type="protein sequence ID" value="ENSXCOP00000018460.1"/>
        <property type="gene ID" value="ENSXCOG00000013896.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114135966"/>
    <dbReference type="KEGG" id="xco:114135966"/>
    <dbReference type="CTD" id="140679"/>
    <dbReference type="GeneTree" id="ENSGT00490000043380"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:inhibitory synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140800">
        <property type="term" value="F:gamma-aminobutyric acid:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015187">
        <property type="term" value="F:glycine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051939">
        <property type="term" value="P:gamma-aminobutyric acid import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006836">
        <property type="term" value="P:neurotransmitter transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Vesicular inhibitory amino acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF689">
        <property type="entry name" value="VESICULAR INHIBITORY AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="307"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="434"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="457"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="491"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="21">
        <location>
            <begin position="116"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="68"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="19">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00034106"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="Pfam" id="PF01490"/>
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            <dbReference type="Ensembl" id="ENSXCOP00000018460.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="526" mass="57714" checksum="490325A25A7D9C76" modified="2018-12-05" version="1">MATLIRGKISNKLSNAATAVSNKSTAKVSGMFARMGFQAATDEEALGFASCDDLDYDHRQGMQMDILSSDEIGGEGGGDGGGLEGDSHYQRDGTGPPHSASKDGGSNNELSEVKPKITAWEAGWNVTNAIQGMFVLGLPYAILHGGYLGLFLIIFAAVVCCYTGKILIACLYEEDEDGQLVRVRDSYVDIANACCAPRFPTLGGHVVNVAQIIELVMTCILYVVVSGNLMYNSFPNMPISQKSWAIIATAALLPCAFLKNLKAVSKFSLLCTMAHFVINVLVIAYCLSRARDWAWDKVKFYIDVKKFPISIGIIVFSYTSQIFLPSLEGNMQKPSEFHCMMKWTHIAACILKGLFALVAYLTWADETKEVITDNLPPTIRAVVNLFLVAKALLSYPLPFFAAVEVLEKTFFQDGGRAFFPDCYGGDGRLKSWGLTLRCILVVFTLLMAIYVPHFALLMGLTGSLTGAGLCFLLPSIFHLKLLWRKLQWHQVFFDVSIFVIGGICSISGFIHSMEGLIEAFKYNIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M630</accession>
    <name>A0A3B5M630_9TELE</name>
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        <submittedName>
            <fullName evidence="6">ST13 Hsp70 interacting protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Actinopterygii</taxon>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
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            <taxon>Xiphophorus</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M630"/>
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        <property type="entry name" value="Hsc70-interacting protein-like protein"/>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Hip_N"/>
    </dbReference>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
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        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45883">
        <property type="entry name" value="HSC70-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45883:SF2">
        <property type="entry name" value="HSC70-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
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    <feature type="domain" description="Hsp70-interacting protein N-terminal" evidence="5">
        <location>
            <begin position="2"/>
            <end position="39"/>
        </location>
    </feature>
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        <location>
            <begin position="129"/>
            <end position="162"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00009015"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF18253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="248" mass="28282" checksum="249583D29D84B454" modified="2018-12-05" version="1">MDPRKVTELKGFVQMCESNPGILHLPEMGFFRTWLQRSGSLKLPGLCTSGETKRYNSSFISEIDNEGVIEPDTDDPEEMGEFDDIEVTEEMMDQANEKKIEAINALGEGDLQKALDLFTEAIKLSPRVAILYAKRASVYIKMQKPNAAIRDCNRAISINPDSAQPYKWRGKAHRYQRATAAVCLAKRHHSVFTSDITKPSVSTQATLLNWFFKHFCLVIFCNIKPECETRKRVLLECLVVFLNLLWFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M708</accession>
    <name>A0A3B5M708_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tuberin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. Within the TSC-TBC complex, TSC2 acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In absence of nutrients, the TSC-TBC complex inhibits mTORC1, thereby preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by the mTORC1 signaling. The TSC-TBC complex is inactivated in response to nutrients, relieving inhibition of mTORC1. Involved in microtubule-mediated protein transport via its ability to regulate mTORC1 signaling. Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Lysosome membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000019260"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019498.1">
        <property type="protein sequence ID" value="ENSXCOP00000019260.1"/>
        <property type="gene ID" value="ENSXCOG00000014479.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183139"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033596">
        <property type="term" value="C:TSC1-TSC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046627">
        <property type="term" value="P:negative regulation of insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051898">
        <property type="term" value="P:negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016241">
        <property type="term" value="P:regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Tuberin isoform X3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Rap/Ran-GAP"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
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        <property type="entry name" value="Rap/Ran_GAP_dom"/>
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        <property type="entry name" value="Tuberin"/>
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        <property type="entry name" value="Tuberin-type_domain"/>
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        <property type="entry name" value="Tuberin/Ral-act_asu"/>
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        <property type="entry name" value="Tuberin_N"/>
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    <dbReference type="PANTHER" id="PTHR10063">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11864">
        <property type="entry name" value="DUF3384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02145">
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    </dbReference>
    <dbReference type="Pfam" id="PF03542">
        <property type="entry name" value="Tuberin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01431">
        <property type="entry name" value="TUBERIN"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111347">
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    </dbReference>
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        <property type="entry name" value="RAPGAP"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
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        <location>
            <begin position="489"/>
            <end position="527"/>
        </location>
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        <location>
            <begin position="1448"/>
            <end position="1675"/>
        </location>
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        <location>
            <begin position="929"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1054"/>
            <end position="1135"/>
        </location>
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            <begin position="1200"/>
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        </location>
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            <begin position="1362"/>
            <end position="1409"/>
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            <begin position="1062"/>
            <end position="1071"/>
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        <location>
            <begin position="1089"/>
            <end position="1100"/>
        </location>
    </feature>
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        <location>
            <begin position="1121"/>
            <end position="1130"/>
        </location>
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        <location>
            <begin position="1202"/>
            <end position="1223"/>
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        <location>
            <begin position="1298"/>
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            <begin position="1387"/>
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            <dbReference type="ARBA" id="ARBA00004514"/>
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    <evidence type="ECO:0000313" key="15">
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            <dbReference type="Proteomes" id="UP000261380"/>
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    <sequence length="1718" mass="189784" checksum="DC6F3D38596EFDFC" modified="2018-12-05" version="1">MNKQQSKETLKEKVKGIFGLGTQRQQTKQCDSKLPEFIITADIIKELHPDCGLSNRIRMMNQICDLAKIKKFEENAVEAVWKAVEDMLSPEQPAEARHAVLQLLRAIIQGQGEWLGPLRAYFFKVIRDYQPCNEDLSERLEVFKALTENGKDITYLEEDIARFVLLWMDIGLTSDFLHVLVNLVKYNSCYLDQNVSSMVQKICLLCNRTTSSTDIEVALQVLDAVVCYNCLPSESLTVFIITLCRTVNVKEFCESCWKLMRKVLGTHLGHSAIYTMCRIMEERVYMEDAPLLRGAVFFVGMALWGAHRLPALKNTPTLVLPSFYKAMSCANEVVSYEIVLSITRLIKKYGKELQVVTWDILLGIIERLLQQIQMIGSAELKAIVYELLTTVEELYEQNGYHGSTEKFFSLVEKCADKRPDASVLTLVSYRAQSIQPAKDGWIQSLHCLMEKFFRNETRSGIRIKVLHILSFVLSTNRQLYEDELIEKVVIPQLSGIAEDRDLAVRKQATQLLVDLAEGCNTHHFTSLLDIIERVASRSLVCSGSLEASDPIADSPMEDVKTAVLGLLEILQSKLYSLPASHASRVYELLISHLQLHYKSKYCSAIASAVRLQVFDFFLMMRADSLHRVGVPNKDGVMRFSPYCYCDAGEPEKRVCDKKPSGSISPPAGGPAAAAAAPPASAASIRSACLPYAPAFSVLLQCLKMETDWKVLKLVLDKLPWTLQYKVLLLTSSCSLDQLCSTLCAMISERLKKMPEGFSRTDVQLAVVPVLTAITSYHSYLDQSRQRELVQCLETGLIYRCAKQCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRLPHLYANFVAEQYVSVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNALLPFDDGHEQSPFRARSTSLNERPKRMHTSTTTCSLGSADENAATQADEGLKTVHLELTETCLDMMARYVFSNFSALPKRSPIAEFLLAGGRSMTWLVGNKLVTITTSGGVRTQALLGMDMSESDPSLHTRMTKEAPAKLESQSSQQQNRTTRTRVRSRSGGHALRAGPIPSLSPLVSPSEGELASPLSPSSGPTLDGIGPPSSTAAALSAPPPLKDNPSLAEFVPMLTQGWAEIFIRRPSGNTSWLMCLENPPSPFSSELGNMPLQELSTVLMAMEGVKEPPTQTASAPASTAAPAPTSSAPLEWVEGEEFEPIPSEPIFMSDKAPLPGPLSRSSSTSSQDDEKSTLEEVSEGAIPIDQPSVGPSTPGSQGPDLPFQSHSQSQGHGLNKSSSSPELQTLPEAFTKAAVESEKSQGDGGEGSGLSSQSGGVAMKLAFPAAPALPGPISPGGGHRPRGHTISVSAPSSRRERKTERDSYHSRPGPSNAEKLSGLSPSFVFLQLYHSPFFGNEANKPLLLPKTQVIDRAVKVLDQMPPYDTHKIGVVFVGAGQVNNEVAILSNEYGSNRYAAFLTGLGKLIHLKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMPNRESDKGCCNKKRHIGNDFVMVVYNDSGEDYKLGTIKGQFNFVEVVIKPLDYECNLVSLQCRKDLEGLVDTTVAKIVSDRNLPLFVRQMALHANMASLVHQYRANPSDAYASKWLARLRHIKRIRTRVSLKTQELQGSPSPTRFSLMFATSAPTPEMSGQRKRLVSTVDDFTDFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7U0</accession>
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            <taxon>Acanthomorphata</taxon>
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        <scope>IDENTIFICATION</scope>
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            <begin position="18"/>
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        <source>
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    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="71" mass="7981" checksum="E68D7B2268E13591" modified="2018-12-05" version="1">MSSSLFLLLSLLVPTLMLEFRYHDNREIEQYLLQVSADNPDITHLYSIGKSVKESYLWAPEGSASPGSSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8B0</accession>
    <name>A0A3B5M8B0_9TELE</name>
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        <recommendedName>
            <fullName evidence="11">PHD-type domain-containing protein</fullName>
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    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
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            <taxon>Poeciliinae</taxon>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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        <property type="protein sequence ID" value="ENSXCOP00000020533.1"/>
        <property type="gene ID" value="ENSXCOG00000015387.1"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Znf_FYVE_PHD"/>
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        <property type="entry name" value="Znf_PHD"/>
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        <property type="entry name" value="Znf_PHD-finger"/>
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        <property type="entry name" value="Znf_RING"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12618">
        <property type="entry name" value="PHD AND RING FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12618:SF20">
        <property type="entry name" value="PHD AND RING FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="113"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="7">
        <location>
            <begin position="192"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="31316" checksum="F3BEB76FD6F1FB15" modified="2018-12-05" version="1">MHRGHVAVHADAGHEADAHVDVGIVQKPSELTGEVSKLPFVLMEVVVDPQGQHAEDDDVRRGQVADVDTKSRSRADPDGEDIQLTSFEVTIMDKLVALISVDGETVDSIADKCYICLCPFEKQTVGSLDQCPHFFCFQCIYQWSQTANTCPVDRISFAFIHQRRCPGGDIQKKLKVTPRRNDNESEEDWRNIVICEECGRSGRTDRLLVCRLCDSGYHKDCLTPPLDRVPEGDWMCPECVITSKNTGIYLMCKMIGSLLLLLLFTHITFHNVCYNGIIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8E9</accession>
    <name>A0A3B5M8E9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Kazal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M8E9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020033.1">
        <property type="protein sequence ID" value="ENSXCOP00000019787.1"/>
        <property type="gene ID" value="ENSXCOG00000014852.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000263903"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00050">
        <property type="entry name" value="Kazal_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00282">
        <property type="entry name" value="KAZAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kazal-like" evidence="1">
        <location>
            <begin position="16"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="56" mass="6055" checksum="CCD5444C993D95E0" modified="2018-12-05" version="1">TQITTLWLIGALSSPEGPKAKCPDPEIFACKLNVRPVCGSNGQTYGNECLLCKEMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8H5</accession>
    <name>A0A3B5M8H5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M8H5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017596"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017819.1">
        <property type="protein sequence ID" value="ENSXCOP00000017596.1"/>
        <property type="gene ID" value="ENSXCOG00000013273.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153498"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990234">
        <property type="term" value="C:transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF55">
        <property type="entry name" value="ALL TRANS-POLYPRENYL-DIPHOSPHATE SYNTHASE PDSS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="306" mass="34019" checksum="2CEF3DA19E1BFB11" modified="2018-12-05" version="1">RSERLRSSIVTSAGSKGLGLFSSSESSSNWSKVVSDAEKIVGYPTSFMSLRCLLSDELSNVAMHVRKLVGTQHPLLNTARGFVYDSKNNLQMRGLIVLLLSKAAGPSHAASELLTKDMVSGIYPSQRNLAEITELIHTAFLVHRGIVNLKEWTVSDGPLKDMEFGNKMAVLSGDFLLANACTGLAELNDTKIVELISSAISDLVQGIYYENYYNVEDQAFLSHGALLAKSCQAAMQLAKHDREAQRLAYLYGKHLSLGHKAKCFMESFDFVLVYQIFFSSLLKNFWRLGRKILRNLISLKTWTEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9Q0</accession>
    <name>A0A3B5M9Q0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Poly [ADP-ribose] polymerase</fullName>
            <shortName evidence="25">PARP</shortName>
            <ecNumber evidence="25">2.4.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="29 30">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="29" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Chromosome</location>
            <location evidence="4">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.-" evidence="25"/>
    <dbReference type="RefSeq" id="XP_027889223.1">
        <property type="nucleotide sequence ID" value="XM_028033422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9Q0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020272"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020520.1">
        <property type="protein sequence ID" value="ENSXCOP00000020272.1"/>
        <property type="gene ID" value="ENSXCOG00000015205.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114154375"/>
    <dbReference type="GeneTree" id="ENSGT00940000156161"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030315">
        <property type="term" value="C:T-tubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904108">
        <property type="term" value="P:protein localization to ciliary inversin compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036371">
        <property type="term" value="P:protein localization to T-tubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055117">
        <property type="term" value="P:regulation of cardiac muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09524">
        <property type="entry name" value="SAM_tankyrase1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01438">
        <property type="entry name" value="tankyrase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000012">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000009">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000010">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000011">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000021">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000024">
        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.228.10:FF:000001">
        <property type="entry name" value="Poly [ADP-ribose] polymerase tankyrase-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24178:SF21">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN 52-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24178">
        <property type="entry name" value="MOLTING PROTEIN MLT-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00644">
        <property type="entry name" value="PARP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="15"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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            <end position="1246"/>
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        <source>
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    <sequence length="1256" mass="135133" checksum="16C6EE469B45FE6C" modified="2018-12-05" version="1">MAVSRRSSQQQQQQTTLQSPPRNGSLSGASPASPPMALLSPAVGSSESERECSGGVDVALASPDLPVPVLVSSASSTSTTSGGGSSSVSSPGSGATSPIDGSSGVVGAFRELFEACRNGDVSRVKRLVDSVNVNAKDMAGRKSTPLHFAAGFGRKDVVEHLLQTGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLLNCHSKSAVDMAPTPELKDRLTYEFKGHSLLQAAREADMAKVKKTLALEIIGFKHPQSNETALHCAVASPHPKRKQVTELLLRKGANINEKNKDFMTPLHVAAERAHNDILEVLQKHGAKVNAADTLGQTALHRAALAGHIQTCKLLLSYGADPSIVSLQGFTAAQMGNEAVQQILNENVPTRNSDVDYRFLEAAKAGDLDTVQQLCSPQNVNCRDLEGRHSTPLHFAAGYNRVAVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNIPLDMVKDGDTDIQDLLRGDAALLDAAKKGCLARVQKLCSPENINCRDAQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTALDLATADDIRALLMDAMPPDALPSCFKPQATVVSASVISPASTPSCLSAASSIDNLAGPLTELAAAAAAGASGVADGASGTDRKEGEMAMLDMNISQFLKSLGLEHLRDIFEREQITLDVLADMGHEELKEIGINAYGHRHKLIKGVERLLGGQQGGNPYLTFHCANQGTILIDLSPDDKEYQSVEEEMQSTIREHRDGGNAGGVFSRYNIIKIQKVVNKKLRERYTHRQKEIADENHNHHNERMLFHGSPFINAIIHKGFDERHAYIGGMFGAGIYFAENSSKSNQYVYGIGGGTGCPTHKDRSCYLCHRQMLFCRVTLGKSFLQFSAMKMAHAPPGHHSVIGRPSVNGLAYAEYVIYRGEQAYPEYLITYQILKPESTAQSAAAAEQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MA31</accession>
    <name>A0A3B5MA31_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MA31"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020655.1">
        <property type="protein sequence ID" value="ENSXCOP00000020407.1"/>
        <property type="gene ID" value="ENSXCOG00000015291.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000240444"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070915">
        <property type="term" value="F:lysophosphatidic acid receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="7tmA_purinoceptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24232:SF95">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="45"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="361" mass="40721" checksum="AF0A89B02496A8FA" modified="2018-12-05" version="1">MELWNVTELEGKPSSPLFNCTVDTSYRFTFYQVSYSVIFLLGLVTNSLALHRLYTSSCTMNSTAVYMASLSFADMFFVISLPMRIYYYHQKITFILKYISLYGGIFFLVCIAVDRYFAVVHPLSPNLRRRRVAQIVSGGIWCVVLGLSVSLPLLRLAASHQNRPCLLDPSSLRHRTFILISLSLVLGSFLLPTLILLYSYSRVLSVLRKSRHRCRSQRRNCRQTLKIIYWVLGVYLLCFLPYHVNLLGYTFTHMKLLPHCGLAKLTKTVHPIVLSLASFNCCLNPLIYYFSSSLVHRETSGGGARDKISPGQDVVLLSFIPACHTQHSEVPLLLSLNVSAHPASGPQCNSDHSNKGHTKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAJ7</accession>
    <name>A0A3B5MAJ7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Leucine rich repeat containing 28</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAJ7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016724"/>
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        <property type="protein sequence ID" value="ENSXCOP00000016724.1"/>
        <property type="gene ID" value="ENSXCOG00000012606.1"/>
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    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF12">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="3">
        <location>
            <begin position="105"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="354" mass="40374" checksum="1581E6D6112A1C62" modified="2018-12-05" version="1">MATELHETIFMAKQERHKNLFLNYKNLNIFPVELLKDEGLQFLERLYMKRNSLTTLPDNLAQKLPNLIELYLHSNNIVIIPEAIGNLARLQSLDLSNNALQMLCPEIGRLRSLRHLRLSNNQLKCLPPEIGDLQDLETLDLAMNQLMSLPDRLHRCLSLQNLTVDHNLLSHVPRQLCWLHRLNQLSMAANRLTFLPLDLGRSRELQFVFVDNNVDLKGLPSYLYNKVIGCSGMGEVLTEALVGLPAEVKVIGSQTDNVVPLEELAMRTMHRIYHHRPAGLNLLPPIILPKSLLDLLQFPLGHCHRCSQAMFTIIYPKLFPLRDTALAGVHRRTTVSFVAYCCSSRCLRTFDLQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBM5</accession>
    <name>A0A3B5MBM5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBM5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017319.1">
        <property type="protein sequence ID" value="ENSXCOP00000017099.1"/>
        <property type="gene ID" value="ENSXCOG00000012893.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183048"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF885">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="57"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="308" mass="34845" checksum="6CEAA90E9A5CD1D2" modified="2018-12-05" version="1">MTNSSQVLFFFLTAYIDSGVLAYLYFTAVLLLYILIIVSNVLLIAVICVNQSLHEPMYMFLCSLFVNELYGSSGLFPFLLVQILSHLHSVAAPFCLLQVFIVHSYGAVEFLNLAVMSYDRYLAICYPLQYNTRMTPTKIAALIAVSWLYPFVAMSFMMSLVPVKLCRNQINKVYCDMHSLVKLACSDAALTNIYGLIATFSTIFISLLLILYSYIKILAVCFSGSKQTRQKAVNTCTPHLASILNFTCAGSFEISQSRFDMSFLPNVLRIFLSLYFLPAKPLFNPLMYGLKMSKIRNICKIKRWGVTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBQ9</accession>
    <name>A0A3B5MBQ9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="37">Choline/ethanolaminephosphotransferase 1</fullName>
            <ecNumber evidence="34">2.7.8.1</ecNumber>
            <ecNumber evidence="35">2.7.8.2</ecNumber>
            <ecNumber evidence="36">2.7.8.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="38">1-alkenyl-2-acylglycerol choline phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="48 49">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="48" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="48" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="31">Catalyzes both phosphatidylcholine and phosphatidylethanolamine biosynthesis from CDP-choline and CDP-ethanolamine, respectively. Involved in protein-dependent process of phospholipid transport to distribute phosphatidyl choline to the lumenal surface. Has a higher cholinephosphotransferase activity than ethanolaminephosphotransferase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>1,2-di-(9Z-hexadecenoyl)-sn-glycerol + CDP-choline = 1,2-di-(9Z-hexadecenoyl)-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54336"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:83717"/>
            <dbReference type="ChEBI" id="CHEBI:84417"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:54337"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>1,2-di-(9Z-hexadecenoyl)-sn-glycerol + CDP-ethanolamine = 1,2-di-(9Z-hexadecenoyl)-sn-glycero-3-phosphoethanolamine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57876"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:84417"/>
            <dbReference type="ChEBI" id="CHEBI:138145"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:54341"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>1,2-didecanoyl-sn-glycerol + CDP-choline = 1,2-didecanoyl-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54236"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18155"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:78226"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:54237"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>1,2-dioctanoyl-sn-glycerol + CDP-choline = 1,2-dioctanoyl-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54232"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:76979"/>
            <dbReference type="ChEBI" id="CHEBI:78228"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:54233"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>1-O-alkyl-2-acyl-sn-glycerol + CDP-choline = a 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36179"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36702"/>
            <dbReference type="ChEBI" id="CHEBI:52595"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:36180"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>1-O-hexadecyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + CDP-choline = 1-O-hexadecyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54352"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:55430"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:77184"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:54353"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>1-O-hexadecyl-2-acetyl-sn-glycerol + CDP-choline = 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54348"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:44811"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:75936"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:54349"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycerol + CDP-choline = 1-hexadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54332"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:74963"/>
            <dbReference type="ChEBI" id="CHEBI:82949"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:54333"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CDP-choline = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54244"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:73001"/>
            <dbReference type="ChEBI" id="CHEBI:75466"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:54245"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CDP-ethanolamine = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57876"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:73007"/>
            <dbReference type="ChEBI" id="CHEBI:75466"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:54253"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="42">
            <text>CDP-choline + 1,2-di-(9Z-octadecenoyl)-sn-glycerol = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54240"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:74669"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="42">
            <dbReference type="Rhea" id="RHEA:54241"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="44">
            <text>CDP-choline + a 1,2-diacyl-sn-glycerol = a 1,2-diacyl-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32939"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="44">
            <dbReference type="Rhea" id="RHEA:32940"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>CDP-ethanolamine + 1,2-di-(9Z-octadecenoyl)-sn-glycerol = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54248"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:57876"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:74986"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="40">
            <dbReference type="Rhea" id="RHEA:54249"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>CDP-ethanolamine + a 1,2-diacyl-sn-glycerol = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32943"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57876"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="2.7.8.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="41">
            <dbReference type="Rhea" id="RHEA:32944"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="43">
            <text>a 1-O-(1Z-alkenyl)-2-acyl-sn-glycerol + CDP-choline = a 1-O-(1Z-alkenyl)-2-acyl-sn-glycero-3-phosphocholine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36227"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58779"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:77286"/>
            <dbReference type="ChEBI" id="CHEBI:77296"/>
            <dbReference type="EC" id="2.7.8.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="43">
            <dbReference type="Rhea" id="RHEA:36228"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="32">Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="33">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 45">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.1" evidence="34"/>
    <dbReference type="EC" id="2.7.8.2" evidence="35"/>
    <dbReference type="EC" id="2.7.8.22" evidence="36"/>
    <dbReference type="RefSeq" id="XP_027858986.1">
        <property type="nucleotide sequence ID" value="XM_028003185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBQ9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018696"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018931.1">
        <property type="protein sequence ID" value="ENSXCOP00000018696.1"/>
        <property type="gene ID" value="ENSXCOG00000014069.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114135685"/>
    <dbReference type="KEGG" id="xco:114135685"/>
    <dbReference type="CTD" id="570720"/>
    <dbReference type="GeneTree" id="ENSGT00950000183117"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047359">
        <property type="term" value="F:1-alkenyl-2-acylglycerol choline phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004142">
        <property type="term" value="F:diacylglycerol cholinephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004307">
        <property type="term" value="F:ethanolaminephosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000002">
        <property type="entry name" value="Choline/ethanolamine phosphotransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014472">
        <property type="entry name" value="CHOPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414:SF27">
        <property type="entry name" value="CHOLINE_ETHANOLAMINEPHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414">
        <property type="entry name" value="ETHANOLAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015665">
        <property type="entry name" value="CHOPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="19" id="KW-0464">Manganese</keyword>
    <keyword evidence="16 47" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="21" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="49" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 45" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 47" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 47" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="47">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="47">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="47">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="47">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="47">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="47">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="47">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="47">
        <location>
            <begin position="379"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="46">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="46">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00035878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00035972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00036059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00036100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00036329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00036341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00036651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00036748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00036890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00037663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00037890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00037891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00038986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00038987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00038994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00039228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00042366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00047335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
            <dbReference type="ARBA" id="ARBA00047910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="41">
        <source>
            <dbReference type="ARBA" id="ARBA00048120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="42">
        <source>
            <dbReference type="ARBA" id="ARBA00048186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="43">
        <source>
            <dbReference type="ARBA" id="ARBA00048188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="44">
        <source>
            <dbReference type="ARBA" id="ARBA00048570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="45">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="46">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="47">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="48">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="49">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="423" mass="47129" checksum="25F883CD15A8F2E8" modified="2018-12-05" version="1">MSITGQHGGGLRTRRSLARDKDQRPGVGMEAACWLSPGVLRRLIELPSPPLSRHQLKRLEEHRYSSAGRSLLEPLMQRYWEWLVGQVPSWIAPNLITIIGLATNVFTTLVLVYYCPTATEQAPLWAYLMCAVGLFIYQSLDAIDGKQARRTNSSSPLGELFDHGCDSLSTVFVVLGTSIAVQLGTNPDWMFFCCFAGMFMFYCAHWQTYVSGTLRFGIIDVTEVQIFIIIMYLLAAVGGSAFWQSLIPVLNIQMKMIPAICTLLGAIFSCTNYFRVIFTGGVGKNGSTIAGTSVLSPVLHIGSVIILAVMIYKKSAVQLFEKHPCLYILAFGFVSAKITNKLVVAHMTKSEMHLHDLAFLGPGLLFLDQYFNSFIDEYLVLWIALIISLFDLVRYCVSVCNQIACHLHIFVFKIKPLLSPRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MEA0</accession>
    <name>A0A3B5MEA0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">5-aminolevulinate synthase</fullName>
            <ecNumber evidence="16">2.3.1.37</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">5-aminolevulinic acid synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Delta-ALA synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Delta-aminolevulinate synthase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), with CoA and CO2 as by-products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>succinyl-CoA + glycine + H(+) = 5-aminolevulinate + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:12921"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="2.3.1.37"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:12922"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Mitochondrion inner membrane</location>
            <topology evidence="16">Peripheral membrane protein</topology>
        </subcellularLocation>
        <text evidence="16">Localizes to the matrix side of the mitochondrion inner membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.37" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MEA0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017994"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018219.1">
        <property type="protein sequence ID" value="ENSXCOP00000017994.1"/>
        <property type="gene ID" value="ENSXCOG00000013553.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156030"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00375"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003870">
        <property type="term" value="F:5-aminolevulinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048821">
        <property type="term" value="P:erythrocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042541">
        <property type="term" value="P:hemoglobin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06454">
        <property type="entry name" value="KBL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000045">
        <property type="entry name" value="5-aminolevulinate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000006">
        <property type="entry name" value="5-aminolevulinate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010961">
        <property type="entry name" value="4pyrrol_synth_NH2levulA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015118">
        <property type="entry name" value="5aminolev_synth_preseq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01821">
        <property type="entry name" value="5aminolev_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF50">
        <property type="entry name" value="5-AMINOLEVULINATE SYNTHASE, NON-SPECIFIC, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09029">
        <property type="entry name" value="Preseq_ALAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9 16" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="11" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 15" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="5-aminolevulinate synthase presequence" evidence="18">
        <location>
            <begin position="3"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="17">
        <location>
            <begin position="217"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU910713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF09029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="611" mass="67939" checksum="E6AF862273D66AE4" modified="2018-12-05" version="1">MEVIVRRCPFLACVPQAFFQQSRKSMVTYAQRCPIMMELASKPMAPSMARALCSSTSNQTTEIKTGNVPVVGVESKLPTGHPVPTTGQAVASKCPFLAAEMKQKNSGVVRQVTMEFQEDVEEVRTLKQPSLTVTSKVGEQSNLMKTLLKQRPKRATHLLQDNLLVSRFQYDDFFEKKIVEKKNDHTYRVFKTVNRLATEFPMADDFTGSLEDKREVSVWCSNDYLGMSRHPRVEKSIMDTLQKHGSGAGGTRNISGTSKFHVELEQELADLHNKDAALLFTSCFVANDSTLFTLAKMLPGCEIYSDAGNHASMIQGIRNSGAKKFIFRHNDVSHLRELLEKGDPTKPKIVAFETVHSMDGAVCPLEEMCDLAHEFGAITFVDEVHAVGLYGARGGGIGDRDGVMHKMDIISGTLGKAFGCVGGYIASTSALVDTVRSYAAGFIFTTSLPPMLLAGARQSIQTLKGEEGRMLRRKHQRNVQLLRQMLMDEGLPVVHCPSHIIPVRVANAEKNTEVCDIMMNRHNIYVQAINYPTVARGEELLRIAPTPHHTPEMMKYFVEKLVQTWKESGLGLKPHSSAECNFCQQPLHFELMSEREKSYFSGLSHPISAHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MED7</accession>
    <name>A0A3B5MED7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AT-rich interactive domain-containing protein 3</fullName>
            <shortName evidence="9">ARID domain-containing protein</shortName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Transcription factor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8 9">Heterodimer with ARID3A. Interacts with unphosphorylated RB1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MED7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021915"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022183.1">
        <property type="protein sequence ID" value="ENSXCOP00000021915.1"/>
        <property type="gene ID" value="ENSXCOG00000016376.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156052"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.60:FF:000008">
        <property type="entry name" value="Putative AT-rich interactive domain-containing protein 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045147">
        <property type="entry name" value="ARI3A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023334">
        <property type="entry name" value="REKLES_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15348:SF30">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15348">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN ARID DOMAIN- CONTAINING PROTEIN DEAD RINGER PROTEIN B-CELL REGULATOR OF IGH TRANSCRIPTION BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51486">
        <property type="entry name" value="REKLES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ARID" evidence="11">
        <location>
            <begin position="227"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="REKLES" evidence="12">
        <location>
            <begin position="418"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="72"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="142"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="331"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="513"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="342"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="513"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="528"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="551" mass="60688" checksum="24DFEFBD322CBF61" modified="2018-12-05" version="1">KVCCTAAKLCSYPSNLSDEGSLQQSVPGGVKLEAVMEQLQRHPGAKLEMNLQEKHFLQAQFLLAHQAVAARGPGGSRSDPLLCGEPDGPIHQTVLNQMEPKQESADGEQREMLEPKEGDELEEEGDERKMSFQHVSHFQPYSTSLPSAAEQKSEPSPSAVKEEQEEKNLLPPVGQSSFTSPNSLADWGYDELFKLVGSGVWTEESDGAKVRGEPSRDFAKLYELDNDPQRKEFLDELFVFMQKRGTPVNRIPIMAKQVLDLYRLYKLVTEKGGLVEVINKKIWREITKGLNLPTSITSAAFTLRTQYMKYLYPFECAKKGLSSPGELQAAIDSNRREGRRPSYTSSLYRYSPSQTSAPHALLPSPTMHTPPTAHNGINMSLSANENSTPVISRLPMALGHHHQQQQHNHPHHQLAQPATLEHLRERLDRPVDGPEKKMMRLAEEQQRLMQQALQQNLLAMASHFNPVSLKLNNGNEKKKDLSLSISTNGPASISVSVEVDGTVYSGTLLAQKTSAPVSSQTVTPAGTSGFSALSCSHSSSFSSSTSSKGPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHV2</accession>
    <name>A0A3B5MHV2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Calcium/calmodulin-dependent protein kinase IGb</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHV2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020701"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020953.1">
        <property type="protein sequence ID" value="ENSXCOP00000020701.1"/>
        <property type="gene ID" value="ENSXCOG00000015517.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156872"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045475">
        <property type="term" value="P:locomotor rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000026">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="21"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="337"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="392"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="348"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="427" mass="48715" checksum="0FEA869D2AC76E79" modified="2018-12-05" version="1">MGRQEGDYAWKKNTENIQEIFEFIEELGSGAFSEVYMVRERKTGKTFAMKCVKKKKKRDINLENEIAVLRKIQHDNVVGMEDLYESRTHYYIIMHLVSGGELFDRILDRGVYSEKDASRVIQQVLEAVSFLHQNGIVHRDLKPENILYYSQDEDSKIMISDFGLSKMAENGIMSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVITYILLCGYPPFYEESETRLFSKIMKAQYEFDSPFWDNISESAKDFIRNMMQKNPSMRYTTEQALRHPWIIGKTARSQDIYYSVSSQIQKNFAQSKWKQAFNAAVAINHMKKLQLAHTELALRQASVPDIKVIDVSSPTKSRKHPDPEKPEAKKEDINGNISETQHMSLPPSHVDLKNHFHPLKASQSQNAAQHAPTMAEQGKHAYAKNQNGKTVQTGVCSIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIU6</accession>
    <name>A0A3B5MIU6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tubulin/FtsZ GTPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIU6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021041"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021299.1">
        <property type="protein sequence ID" value="ENSXCOP00000021041.1"/>
        <property type="gene ID" value="ENSXCOG00000015745.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182825"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000079">
        <property type="entry name" value="Tubulin, alpha 4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36527">
        <property type="entry name" value="OS01G0282866 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36527:SF6">
        <property type="entry name" value="TUBULIN_FTSZ GTPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="11">
        <location>
            <begin position="3"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="82" mass="8786" checksum="AC560239AB365916" modified="2018-12-05" version="1">QRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTLGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTEPVLVSPPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJ82</accession>
    <name>A0A3B5MJ82_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Pore-forming subunit of a voltage-gated sodium (Nav) channel that directly mediates the depolarizing phase of action potentials in excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na+ ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Voltage-gated sodium (Nav) channels consist of an ion-conducting alpha subunit which is functional on its own associated with regulatory beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Cell membrane</location>
            <topology evidence="1 20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.4/SCN4A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000024393"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024687.1">
        <property type="protein sequence ID" value="ENSXCOP00000024393.1"/>
        <property type="gene ID" value="ENSXCOG00000018212.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159417"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000002">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000001">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000002">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000004">
        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000003">
        <property type="entry name" value="Voltage-dependent sodium channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000049">
        <property type="entry name" value="Voltage-dependent sodium channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1190">
        <property type="entry name" value="iswi atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF223">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN TYPE 4 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 20" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 20" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="14 20" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 20" id="KW-0813">Transport</keyword>
    <keyword evidence="7 20" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="389"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="549"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="580"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="622"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="675"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="715"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="752"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="897"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="936"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="964"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1028"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1138"/>
            <end position="1162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1200"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1239"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1273"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1337"/>
            <end position="1361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1429"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="21">
        <location>
            <begin position="152"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="21">
        <location>
            <begin position="228"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="21">
        <location>
            <begin position="552"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="21">
        <location>
            <begin position="895"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="21">
        <location>
            <begin position="1208"/>
            <end position="1462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00038083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00055248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00064899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1658" mass="191560" checksum="A74D0F5B06B65C0F" modified="2018-12-05" version="1">MVPLLPPVGTEVFRCFTPASLEKIQRKDEAEAKEQQIRKDKNIKIAKKYLPKPAGDLEDGKPLPLFYEDQAQEFLNIPLEDMDPFYQSHKTFIVLSKGKTIYRFNAEPTCYLLSPFNFLRIFSIRILIHSYPLCLYHVTMILLRTLFLISQYFFIMATILTDCVFMTMSNPPAWSKTVEYVVMVIYTFEVIIKVLSRGFCVGDFTYLRNPWNWLEFMVINMMNVSVLQIPSLRTIFGAVIQSVKKLRDAMLLTFFFLSVFALIGLQLFMGDLRQKCVFTLSSDSNTSGAFNISSYENNGSDFDFYEYINNPENHYYPPVQLDALLCGNSSDAGTCPDGYMCLKAGRNPNYDYTSYDSFSWAFLSLFRLMTQDFWENLFHMTLRAAGKTYIIFFVVVIFLGSFYLINLILAVVVMAYAEQNRANIVKAKQREKEYARILKELKKREEKVQCDGEKASITFQYKTEYLFSLHQTVQRATPLEKNGSPQGLWPSKKYLIKNPKLLGHVLCVSDLEDNQKPRSSRWYVFADTFLKWDCCGCCRWLKKLLHTTVMDPFVDVAITMCIVLYTILMATEHYYMTEKLLQLIEIGNLVITGILTAEMVLKILAMDPYHYFQEGWNIFDSIIVTMSLVELGLAYVYSVVWRPLRLIRVFKLAMSWPAFKVLIKIIRNSLRALGNLMLVLAIIVFIFTVFGMQLFEKSYKDCVCRIAQDCELPRWHMSDFFHAFLVIFRILCGEWIETMWDCMQVAGKPMCLIVYMMVLVIGKLVVLNLFIALLLSSFSGDNLAVTEDEGENNLQIVINRISRGTARTKAWFWTLVGKKTNVNSDVNGGNRYLLKHKHYDIERFPVISIVKEAEDTPDDCFTESNCCPCLDIDTSKSIGRIWLYIRKTCITIVESKYFKSFIITVILLSSAALAFEDIYIEQRQDIKIILEYADQVFNFVFVVEIILKLGAYGCKTFFSSAWCWLDFLIADVSLVLLSASILGYSELGAIKSLRTLRALRPLRVLSCFEGLRVMVNELVGAIPSIFNVLLICLVLWLIFSIMGVNLFAGKFFYCFNETSEEYFSPEEVNNKSQCLDLFEKGFSEVRWKISKINFDSVVMGCLSLLQVSTFKGWLDIMYSAVDSREVEAQPMYESNLYMYMYFVCFIIFGSFFPFCLFITSLINFNQCKPKISFFSMEIEPPIKWIPREPPSRPTNKFQGLVFDLVTNPFFDILIMVLICLYTLAMMVETDEQSPEKEEILYWVNLVFIMIFSIECCLKIIALRKHYCYDGWNIFELVVVILCIVGFVVADIIEKYFVSPTLFRVIRLARIGLILRLIRRAKGIRKLLFALGKSLPAVFNLCILLFIIMFVFSIFGMSNFAYVKKDSMIDDMINFETFGNSMISMFTITTLAGWDGLLSPILNTPPDCDPDFENPGTSVRGNCASPAAGIVFFTLYVIVSFLVVVNIHITIILEIFNAAAEESTNKLSEYDIDMFYKTWEKFDPQASQFIHYSELSDLYDALPYPLRIPKPNTINLLQMDLPLVQGDRVHCLDVLLALTEEVFGESQATEELKSTVEWKFMANIASEESLRPIVSTFRKKHEEMAATAIQRAYRRHVQQRVNKQESYQDFSVNQWISCTEAVLKLFNRRPAFYQMRKVIPNFRYPITEKKTDLYINLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJ96</accession>
    <name>A0A3B5MJ96_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transmembrane protein 100</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">TMEM100</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJ96"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024408"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024702.1">
        <property type="protein sequence ID" value="ENSXCOP00000024408.1"/>
        <property type="gene ID" value="ENSXCOG00000018228.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154322"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071773">
        <property type="term" value="P:cellular response to BMP stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032536">
        <property type="entry name" value="TMEM100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16100">
        <property type="entry name" value="PHOSPHOINOSITIDE-INTERACTING PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16100:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16311">
        <property type="entry name" value="TMEM100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="89"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="139" mass="15128" checksum="BA34937ECD38B9FA" modified="2018-12-05" version="1">MLQFWSPNELNKDAMKTPATSEKPKAPEQPPVAMTTLNIPLVNEVQLTAATGGAELSCYRCTVPFGVVVLIAGIVVTAVAYSFNSHGSTISYFGLVLLSAGLVLLASSAVCWKVRLERKKERRRESQTALVTNRRSIFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5ML55</accession>
    <name>A0A3B5ML55_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5ML55"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024483"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024777.1">
        <property type="protein sequence ID" value="ENSXCOP00000024483.1"/>
        <property type="gene ID" value="ENSXCOG00000018288.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163849"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019814">
        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050199">
        <property type="entry name" value="IgHV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23266">
        <property type="entry name" value="IMMUNOGLOBULIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="35"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="120" mass="13774" checksum="1CE820733CAFCD21" modified="2018-12-05" version="1">MCVRVEDLLTKNFSLTGVFSEIKLDQSQSEGKRPGETVKMSCVMSGFDMTEYFIHWIRQKPGRALEWIGRMDTGTDYGSSFQSCFIMTENVPSSTQYLEIRSLTPEDTAVYYCVRRDTVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLI1</accession>
    <name>A0A3B5MLI1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLI1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024431"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024725.1">
        <property type="protein sequence ID" value="ENSXCOP00000024431.1"/>
        <property type="gene ID" value="ENSXCOG00000018242.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154334"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19809">
        <property type="entry name" value="Bbox1_TRIM45_C-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF291">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="20"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="11">
        <location>
            <begin position="112"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="11">
        <location>
            <begin position="168"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="9">
        <location>
            <begin position="356"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="544" mass="59872" checksum="E20699E5368D5D54" modified="2018-12-05" version="1">MRKISLCKQQHAGGLPRAVCSVCKHLYREPKILPCLHTFCSDCIGQLEPFSVSPRGRFTSPEAAEQHRDSLSFTVLCPECDTEVDIPQSGPAGLSTDHLALDEVFLETLVSDGPLGCDLCGEGSAESRCEVCSLNLCEFCRQAHRRQKRTASHSVQAVTELKARGRLCRPVLCSLHPGQELRLFCQPCDLPVCLECAATLHRDHRCCPTHDVIDRHGDRIRELVSARLRPRLERLEDSLQKVEISQESLQVRVDETANQIRAFAREYASAVEAHCLSLLRRLEEIRAQRRNHLLLQGAQLQQALLDVRGSVEFAERLLSCGSDAEILSAKGVTLRRLTSLPAGEVEGYPLVGLILSRTVDVSRCTVEGEGLQQGTVAQQGHFTLVCRDSGGERLARGGEQVLVSVIHKEKKNCTVETIVVDNSDGSYSVSYRPEEPGEYSVWVCVRAQHVKGSPFILNVQRKFRQHNGTFHCCSFCSSGGAKEARCGCPGTMPGGFKGCGHGHKGHPGKPHWSCCGNTAEKSECLPQSILAAVSPHRHLRTVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MM20</accession>
    <name>A0A3B5MM20_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MM20"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020524"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020774.1">
        <property type="protein sequence ID" value="ENSXCOP00000020524.1"/>
        <property type="gene ID" value="ENSXCOG00000015379.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230587"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054148">
        <property type="entry name" value="ASNSD1-SEP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21975">
        <property type="entry name" value="ASNSD1-SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="104" mass="11904" checksum="C2716A03AFE4DFEB" modified="2018-12-05" version="1">MLSSFSRTEAPAGALVKIVPTKTCVPQREWRTVKEQKIIVDELSNLKKNRKVYIQQRNSNVFFLADRNQTLSSCKSKSFFSGSCLLTLMDRDPGPFCLQRSWTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MR73</accession>
    <name>A0A3B5MR73_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Nuclear pore complex protein Nup153</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">153 kDa nucleoporin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Nucleoporin Nup153</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the NUP153 family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000026914"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027244.1">
        <property type="protein sequence ID" value="ENSXCOP00000026914.1"/>
        <property type="gene ID" value="ENSXCOG00000020098.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153253"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1060.10:FF:000001">
        <property type="entry name" value="Nuclear pore complex protein Nup153"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026054">
        <property type="entry name" value="Nucleoporin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013913">
        <property type="entry name" value="Nup153_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23193">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23193:SF23">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08604">
        <property type="entry name" value="Nup153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="13" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="22">
        <location>
            <begin position="644"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="22">
        <location>
            <begin position="689"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="22">
        <location>
            <begin position="758"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="22">
        <location>
            <begin position="811"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="50"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="172"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="380"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="489"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="602"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="944"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1275"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1342"/>
            <end position="1372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1388"/>
            <end position="1409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="57"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="21">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="141"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="397"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="421"/>
            <end position="432"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="21">
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            <begin position="489"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="21">
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            <begin position="609"/>
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    </feature>
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        <location>
            <begin position="944"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="976"/>
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        </location>
    </feature>
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        <location>
            <begin position="1002"/>
            <end position="1016"/>
        </location>
    </feature>
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        <location>
            <begin position="1030"/>
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        </location>
    </feature>
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            <begin position="1067"/>
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        </location>
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        <location>
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        <location>
            <begin position="1399"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00060842"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00068609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00078197"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00079437"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
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            <dbReference type="Ensembl" id="ENSXCOP00000026914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1409" mass="143220" checksum="72532F1A2419D1BD" modified="2018-12-05" version="1">MAATGGGKIRSRRYHIASKPYAKSKQQSGLISRVTDTVKSIVPSWLQKYFKNGDTPAGQTGQGTEQNCQPTPPPAPPNGNEEGPPPIDGRYSPEPSTSNTEPSTSRASLNFQEYVLSRPPLSRSHLHFTPLDASSPTMGASSSLFSPPSTSSAPGPFSTGFSLVKEIKDNLSQHEDDNISTTSGFSSRASDKDVPTSKTASLPQLWSPEMDRTNSGPQQSQSSLKKPSFNLSVFGNSSNSTLNSTLLNSSQLGDSPFYPGKTTYGGAAAVRSSRGRPGTPYQAPVRRQIKAKPAGAQPCGVTSATARRILQSLERMSSPLADAKRIPAATAGSPSRSPARLAHCLVIYFLSVLLPFPQMDSNLPPVQKLVVPAAASVSGNRSVSFRPTLTPGGLTRALDRTPREKTPTRQSPQSFSDPVYPLSSTPAASSLSSGGGKIRRERTTARPSSKHTEEDEVAEAPVLPSISLPINKSSLPTFSFSPSLPPAISVSSSPSFTSTTPAKETLANKDPPKASTPPCAPFTFSSPIVKATAASPPSYSPAVGFTFSAPVAKLAPSMSNGNLTAPTSTEEFVGPFKPAKTLKQGSVLDLLKSPGFASPVVQNSLGTNTASQQTPTQPTTTPSPGTSFSSSLPSPSGFGGLPSASAGWSCDVCLVQNKQSEIKCVCCQAPKPNSSPKSAEIFGPIFSKPAGTWECDTCLVLNKSDAVKCVACETAKPGTGLKPSLTFPPNFSAAKTASTPSDPVSTGISGFGDKFKKPEGAWECETCLVQNKAEDTKCVACTASKPGASAAVSSAASSAPTFALLEQFKKPEGSWECDTCMVQNKAADVKCVACQTTKPGATVEPKGKGRRFIFGTSDSTAGSGSFKFGTTDSSLSSSSGGFKFGASFGSSSSESSSKDTTASSGFKFGGSSEGFKFGAASNEDKKSDQPAAGAGLKFGGSSEVAFGSGSSSTESNSSSKSGFTFGLTKSADKTPETTTTSSSSITLTPLTSSRGNSDKAGSSNDTTSTNTTTATAKGSVFGRLGDPNVASGTPQVSSTFGSLQPEEKKEAAPSTFVFGAVKKEPEAAAAPSGGGFSFSKPSAPADQPPPAFTFGKQAEKSETSTSEASKPSFSFGQTAADSTAAPKPAFSFLANNPPSATTSSSATPTPAMFGAASSGSTPAPDATSSSSSSAPSTFVFGQPSSTTGDAPPAKNFMFGQNHDNQSAAPSSAPLNPAQPFIFGASANAASAPAPAAPSFTFGAPAAAPPAASSSAPSAAPSPFVFSSAPSGGFGANQNPPFASGFGSPFPANPTQTFGAKPNAAPTFGQQNNATPVFGSSGGFQFGGASAFGASNNSSGVFTFGPGAGAPSAPSANPPIAPQSGAAGGSFNFTQTPAFNIGSAKTFTASPAGQSGIAGRKIKTAVRRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRL8</accession>
    <name>A0A3B5MRL8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Electron transfer flavoprotein subunit alpha</fullName>
            <shortName evidence="9">Alpha-ETF</shortName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism.</text>
    </comment>
    <comment type="function">
        <text evidence="9">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="9 10">Binds 1 FAD per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer composed of ETFA and ETFB. Identified in a complex that contains ETFA, ETFB and ETFRF1. Interaction with ETFRF1 promotes dissociation of the bound FAD and loss of electron transfer activity. Interacts with TASOR.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRL8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027054"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027387.1">
        <property type="protein sequence ID" value="ENSXCOP00000027054.1"/>
        <property type="gene ID" value="ENSXCOG00000020206.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013422"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045251">
        <property type="term" value="C:electron transfer flavoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031998">
        <property type="term" value="P:regulation of fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018206">
        <property type="entry name" value="ETF_asu_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153:SF1">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00696">
        <property type="entry name" value="ETF_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 9 10" id="KW-0274">FAD</keyword>
    <keyword evidence="4 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="226"/>
            <end position="227"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="240"/>
            <end position="244"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="257"/>
            <end position="264"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000089-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="310" mass="32338" checksum="8A40E19E8207D275" modified="2025-10-08" version="2">MVVAEHNNDKLTAITLNAITAAGKLGGDVSCLVAGTNCAKVVEEISKVQGVKKILVAQHDSYKGALPEELTPLILETQKQFNFTHICAGASAFGKNLLPRVAAKLDVAPVSDIIEIKSPDTFVRTIYAGNALSTVKCNEAVKVFTVRGTAFEATSAEGGSAASVEVASTPPAGVSEWLEQNLTKSDRPELTSAKVVVSGGRGLKSGENFKLLYDLADKLNAAVGASRAAVDAGFVPNDMQVGQTGKIVAPDLYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGLVADLFKAVPEMTEALSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRT8</accession>
    <name>A0A3B5MRT8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dynein regulatory complex subunit 7 MORN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DRC7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRT8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022209"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022478.1">
        <property type="protein sequence ID" value="ENSXCOP00000022209.1"/>
        <property type="gene ID" value="ENSXCOG00000016591.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166641"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033551">
        <property type="entry name" value="DRC7/lobo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056291">
        <property type="entry name" value="MORN_DRC7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35249">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35249:SF2">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24667">
        <property type="entry name" value="MORN_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein regulatory complex subunit 7 MORN" evidence="8">
        <location>
            <begin position="382"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="534" mass="61316" checksum="49FE8DCCFC3A3339" modified="2018-12-05" version="1">MFNPNISDIFMYPAAVDFLNLKTTGLETLHCSLLCLKLKAFYEVSESRPLLSVCSPESYKTNSQKEEHLLEIAENFQRQYLLLHPDRKPLLLYPVNEYGVRKFVSTTLRPTETGLWELQSWQECSSLVADFLSLQLLEPPTLLFSPTAVLRSQKATSFESSTLLCSLLLGLNYDAYVVSGYAAREMCLLDLSLQQCPLLDTKAKGETSDQTQKKYGVKCPMELTSRFLLDQEKKKLEAQDAAILEKELQEQEASIHLPDPLNGFRVHCWVLVLSGRRSIEENFFIDPLTGCSYPTVHDSFQGIESLWNPFNYYVNRQICHAGCKDINFDLENSTQWEPVLHDGVSQQQLIQAVHRRMEKFFLMRSWVSRIGITRKGETTRFPGGKKVTRYRKAMLERFSSTVTNDGLISRLTTYQDLSCTKVARVKEWYKYRSDGLEEREFNQLENSTTERFKSGRAFDLLCKSTAKRSNQEELTGCNWLLVDMAEEPPGPLSWYRLVKYLISEEEKIALQIKASLVEVTTGSHQIFYPSGFLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MU20</accession>
    <name>A0A3B5MU20_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MU20"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027253"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027586.1">
        <property type="protein sequence ID" value="ENSXCOP00000027253.1"/>
        <property type="gene ID" value="ENSXCOG00000020342.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159196"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05246">
        <property type="entry name" value="dTDP_GD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005888">
        <property type="entry name" value="dTDP_Gluc_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="46"/>
            <end position="344"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00001911"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
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            <dbReference type="ARBA" id="ARBA00023239"/>
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        <source>
            <dbReference type="Pfam" id="PF16363"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027253.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
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    </evidence>
    <sequence length="374" mass="43133" checksum="DA9E26B535D685A7" modified="2018-12-05" version="1">MIKAEIYEEPFASVFCYNACDVQISRAGHCDVLNFNWFSNFDICSGSHLVCSLVASYPEWRIINLDILDYCCSPRSLEGLENRDNYTFIQGDVCNPQLVNHIFITENIDVIFHLAAKTHVESSFEIPSVFQRVNVDGTRVLLDAAHRARHQPQRFVYVSTDEVYGTSLDEAFDEDCPLRPTNPYAATKAAAEYLVRSYWDKYQFPIIITRSNNIYGPRQFTEKVIPRFLTLLQNNNKCTIQGTLAMSRHFLFVSDAVQAFLLVLEKGTVGDVYNVGTSVEIPIVQLARELVRMVKNVAESEVNDWIEFVSSRPQVDLRYPIRSEKLQRLGWRAETSWAEGIRQTVKWYQDNPDFWLDVNKDQRIRKEPEKASNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MU43</accession>
    <name>A0A3B5MU43_9TELE</name>
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        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nematocyst</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Target cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MU43"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027349.1">
        <property type="protein sequence ID" value="ENSXCOP00000027016.1"/>
        <property type="gene ID" value="ENSXCOG00000020181.1"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:nematocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:other organism cell membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Cytolysin/lectin"/>
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        <property type="entry name" value="ACTINOPORIN-LIKE PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR40388">
        <property type="entry name" value="BRYOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63724">
        <property type="entry name" value="Cytolysin/lectin"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0166">Nematocyst</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1052">Target cell membrane</keyword>
    <keyword evidence="4" id="KW-1053">Target membrane</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="194" mass="21416" checksum="F4BB3397A5F43C45" modified="2018-12-05" version="1">NLYSFLRNIMEVINVAADVVMASITVTQAIINSVPSRECNIEITNFSDLYVLKNPGMFLCRGVCVKPLPTRILPSGCGGAFFAKTPRTRFGFSGVITYDLLNSSTGKITEQMALFFKVPYDLNFKANVHAVGVFDVCKGCKVNLHEEMANKTDVTFIRGNAKGPSLTHKSKDVTISATIFVSWICWNLKMGENM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MU49</accession>
    <name>A0A3B5MU49_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MU49"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027934"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028270.1">
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        <property type="gene ID" value="ENSXCOG00000020833.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="4"/>
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        <property type="entry name" value="neural cell adhesion molecule L1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="neural cell adhesion molecule L1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Neural cell adhesion molecule L1-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000363">
        <property type="entry name" value="neurofascin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Neuronal cell adhesion molecule"/>
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    </dbReference>
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        <property type="entry name" value="Neuronal cell adhesion molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
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        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026966">
        <property type="entry name" value="Neurofascin/L1/NrCAM_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF15">
        <property type="entry name" value="NEURONAL CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13882">
        <property type="entry name" value="Bravo_FIGEY"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="fn3"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
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        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="IGc2"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 19" id="KW-0732">Signal</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017443535" evidence="19">
        <location>
            <begin position="23"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="1028"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="33"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="131"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="234"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="327"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="421"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="512"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="606"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="703"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="804"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="910"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="689"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="893"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1089"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="893"/>
            <end position="905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="914"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1149"/>
            <end position="1158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008588"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1158" mass="128768" checksum="7C2FE1E73EE50C74" modified="2018-12-05" version="1">MMEKRSLEAALLVLILGHLVSALEVPLDLAQPPTITHQSPKDYIVDPRENIIIHCEAKGKPHPSFSWTRNGTHFDIEQDESVTLRPHSGTLVVDISRARPEHYEGVFQCTARNKHGSAVSNNIVVRQSKSPLWSKEKNKPIVVQEGVSLVLPCRPPAGLPPPIIFWMDNNFQKLPQGKRVSQSLNGDLYFSNVRREDSRNDYICYARFTHTQTIQQKQPITVKVLNRKSLPPSPTFLIPSGHSSYKMVLRGHVLEMECIAEGLPTPEVSWTKVSGDLPSRRTSFLHFNKTLRIVNVSESDEGDYSCTAKNQLGSAHHIINVEVKAAPYWISGPPKNLVLAPGENGALTCRASGTPKPSIIWSMNGDRIENLPTDISRKVEDDTIIFTDVQVGSSAVYQCNISNPYGYLLSNAFVNVLHESPRVLTPANKIYQVIENRPALMDCSSFGSPIPKIEWFKDSRASTLNGDPYILHDNGTLEIQVAQSQHKGKYTCIARNSLGASENHVYLHIKEPTRILKQPVDTVVQRGRSVTFECTIKHDKDLLPTLIWLKDDEELPDDERFIVEFNSLYITDVSERDAGVYTCYVNTTLDQDSVTAELTVVGRPDPPTDLELTDQKKRSIQLTWIPGEEHNSPIQEFVIQYEDSLHHQGHWHDIARVPGTKTTAHLKLSPYVHYTFRVLARNAVGLSEPSSTSRMYKTEPAAPDQNPKNVRGSGNKHNNLVITWEPLSGLQSNGPGLYYKVSWRQKRPGSDWITATTNNSMYTVSEIPTFSPFEIKVQAVNNHGDGPDPTVAIGYSGEDLPAAAPANVQAVVLNSTLARVRWDPVPPHLIRGQIQGYKVHYSRTHSLHKHNPHHTEKRMLTFSGNHSQGLLPGLHPFSHYSFYVSVFNKKGEGPKSEKQEFKTPEGEPGPPASLSVTNPSLDSLTLEWSPPHEHNGHITGYNLKYQPVNNSNELGPEEELALASNETSVTLQNLKYSPRYKFYLNARTSVGAGPAISQEAVVKQDEGKSSKADKSVTSRQVDIATQGWFIGLMCAIALLILILLIICFIQRNKGGKYPGESLHKQTQCFCSQNEGYFYCCDHKLVKESRTPSNGTVKRDDSDDSLVDYGEGGDGQFNEDGSFIGQYSGKSSSRDNAEGPESSGAPSPINAMNSLNSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MV48</accession>
    <name>A0A3B5MV48_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sal-like protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Transcriptional repressor involved in organogenesis. Plays an essential role in ureteric bud invasion during kidney development.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">May associate with NuRD histone deacetylase complex (HDAC). Interacts with components of HDAC complex including HDAC1, HDAC2, RBBP4, RBPP7, MTA1 and MTA2. Interacts with CCNQ. Interacts with NSD2 (via PHD-type zinc fingers 1, 2 and 3).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the sal C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MV48"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025162"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025467.1">
        <property type="protein sequence ID" value="ENSXCOP00000025162.1"/>
        <property type="gene ID" value="ENSXCOG00000018799.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159356"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048646">
        <property type="term" value="P:anatomical structure formation involved in morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048699">
        <property type="term" value="P:generation of neurons"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003337">
        <property type="term" value="P:mesenchymal to epithelial transition involved in metanephros morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035295">
        <property type="term" value="P:tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20908">
        <property type="entry name" value="SUF4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002381">
        <property type="entry name" value="Putative spalt protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000689">
        <property type="entry name" value="Spalt like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000025">
        <property type="entry name" value="Spalt-like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000079">
        <property type="entry name" value="Spalt-like transcription factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000215">
        <property type="entry name" value="Spalt-like transcription factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000130">
        <property type="entry name" value="Spalt-like transcription factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051565">
        <property type="entry name" value="Sal_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23233">
        <property type="entry name" value="SAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23233:SF46">
        <property type="entry name" value="SAL-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="285"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="313"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="506"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="534"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="566"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="768"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="796"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00069282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="919" mass="99897" checksum="9D122A0730839C3F" modified="2018-12-05" version="1">MDEGQEKLFFCRRPGNLEKDCKLSRSSEEMHICARCCAQFFTWTDLIRHQGVCTEEPMVLVVKDDLPEESPGEASPLPTSAAGRMPGTNVTLEILHNTRVAVAQFSQGMKSSGLGEKATSAAIPVILEHLLALQQQQVHQLQLIEQICSQVAVMKRTMPSSINGQAAVSLPAVLEKPQSLPSHSFCGKSALMSSCPESSPPPICSSMLLPPCSDSSSISSSSSSLPFLPQSPPSGVIFPNPLASIAATANALDPLAALIKQRKGKLPNLPLFEAKPSPEESFFKHKCRFCAKVFGSDSALQIHLRSHTGERPFKCNICGNRFSTKGNLKVHFQRHKDKYPHVQMNPFPVPEYLDNVPTSSGIPYGMSVTPEKPVSSWLDSKLLVATLPATLALPLSSTIPRVGGSTDPVNVPALVKSPYQAAPGECVSDPLNQRGIEAGFSPVPETDASKILKTDGVQLPLKLNTSASFASSSPPVLHLTDSLQTSETSKLQQLVENIDKKISDPNQCILCHRILSCQSALRMHYRVHTGERPFKCKVCSRAFTTKGNLKTHVTVHRETPPVQVQHSCPICEKKFTNAVALQQHIRLHMVGQIPDMMERLQDMDSYESLSSNGNELTDDFFMDDEEEEEENMEEDINSLKPFISPTSAPDPSKAWLKDESQYRVSFQLSRDRGKTNVTCLDVNILKTLHLLTVRSVLSATDQSVSSLIHGSSSGSAKAEVSGQTQLNGMMDVHHLPPFTLNISASYPATSSPRAASLLGLPRRTPKQHNCNACGKNFSSASALQIHERTHTGEKPFVCSVCGRAFTTKGNLKVHMGTHMWSGGPARRGRRLSLENPLALLGGEALVLQKDLANRAVDSGFWNRYATAITSSLAMKSNEISVIQNRRVSQLHPLSIAAAGPIAGLNGTGIDLGSNRHFSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MXL2</accession>
    <name>A0A3B5MXL2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C1q domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MXL2"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026737.1">
        <property type="protein sequence ID" value="ENSXCOP00000026417.1"/>
        <property type="gene ID" value="ENSXCOG00000019723.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163520"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001073">
        <property type="entry name" value="C1q_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050822">
        <property type="entry name" value="Cerebellin_Synaptic_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923:SF102">
        <property type="entry name" value="CEREBELLIN 13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22923">
        <property type="entry name" value="CEREBELLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
        <property type="entry name" value="C1q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00110">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017250861" description="C1q domain-containing protein" evidence="4">
        <location>
            <begin position="19"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="C1q" evidence="5">
        <location>
            <begin position="37"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="183" mass="19855" checksum="A73AB9B46A570020" modified="2018-12-05" version="1">MKSSLFLVAVLVCGLVGAQQGGLPERVQKLEADNDVLTKSRVAFSAALVDTNDWTAKGPLEADETLKFERVITNIGNGYDPATGGRDSLRSVRLYYIQVTGIAGPNGELHAGVKKNGLNMFAIYHKARTQAGASNAMVLVLEQGDRVYVQLWAGKTIVDQGRLSTFTGFLVFPITTQLRQIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MY62</accession>
    <name>A0A3B5MY62_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Guanine nucleotide-binding protein G(i) subunit alpha-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Adenylate cyclase-inhibiting G alpha protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="23 24">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="23" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. May play a role in cell division.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-alpha family. G(i/o/t/z) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027858725.1">
        <property type="nucleotide sequence ID" value="XM_028002924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MY62"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026787"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027116.1">
        <property type="protein sequence ID" value="ENSXCOP00000026787.1"/>
        <property type="gene ID" value="ENSXCOG00000020008.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114135550"/>
    <dbReference type="KEGG" id="xco:114135550"/>
    <dbReference type="GeneTree" id="ENSGT00940000155125"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007193">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001973">
        <property type="term" value="P:G protein-coupled adenosine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007214">
        <property type="term" value="P:gamma-aminobutyric acid signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000001">
        <property type="entry name" value="Guanine nucleotide-binding protein G(I) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002487">
        <property type="entry name" value="Guanine nucleotide-binding protein G(i) subunit alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003559">
        <property type="entry name" value="Guanine nucleotide-binding protein G(i) subunit alpha-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001408">
        <property type="entry name" value="Gprotein_alpha_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF73">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN G(I) SUBUNIT ALPHA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00441">
        <property type="entry name" value="GPROTEINAI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 21" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10 22" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0519">Myristate</keyword>
    <keyword evidence="9 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0564">Palmitate</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0807">Transducer</keyword>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="151"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="176"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="201"/>
            <end position="205"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="270"/>
            <end position="273"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00037181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00039891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="355" mass="40753" checksum="2710921E8B888987" modified="2018-12-05" version="1">MGCTVSQEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECKQYRAVVYSNTIQSIMAIIKAMSNLKIEYEDSSRTDDARQLFSLSADAEEQGSLPEDLSKVIQRLWEDGGVQSCFTRAREYQLNDSAAYYLNDLERIAKSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTETSIILFLNKMDLFEKKIEHSPLSICFPEYTGLNNYKEAAAYIQSKFEELNKKKETKEIYTHFTCATDTENVQFVFDAVTDVIIKNNLKDCGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MYG1</accession>
    <name>A0A3B5MYG1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">PR domain zinc finger protein 1</fullName>
            <ecNumber evidence="13">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Transcription factor that mediates a transcriptional program in various innate and adaptive immune tissue-resident lymphocyte T cell types such as tissue-resident memory T (Trm), natural killer (trNK) and natural killer T (NKT) cells and negatively regulates gene expression of proteins that promote the egress of tissue-resident T-cell populations from non-lymphoid organs. Plays a role in the development, retention and long-term establishment of adaptive and innate tissue-resident lymphocyte T cell types in non-lymphoid organs, such as the skin and gut, but also in other nonbarrier tissues like liver and kidney, and therefore may provide immediate immunological protection against reactivating infections or viral reinfection. Binds specifically to the PRDI element in the promoter of the beta-interferon gene. Drives the maturation of B-lymphocytes into Ig secreting cells. Associates with the transcriptional repressor ZNF683 to chromatin at gene promoter regions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with PRMT5. Interacts with FBXO10. Interacts with FBXO11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the class V-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MYG1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025345"/>
    <dbReference type="Ensembl" id="ENSXCOT00000025652.1">
        <property type="protein sequence ID" value="ENSXCOP00000025345.1"/>
        <property type="gene ID" value="ENSXCOG00000018920.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154798"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002682">
        <property type="term" value="P:regulation of immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051239">
        <property type="term" value="P:regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19187">
        <property type="entry name" value="PR-SET_PRDM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000132">
        <property type="entry name" value="PR domain zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000262">
        <property type="entry name" value="PR domain zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001272">
        <property type="entry name" value="Zinc finger protein 683"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016608">
        <property type="entry name" value="PRDM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044413">
        <property type="entry name" value="PRDM1_PR-SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF64">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21549">
        <property type="entry name" value="PRDM2_PR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF013212">
        <property type="entry name" value="PRDM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0391">Immunity</keyword>
    <keyword evidence="2" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="17">
        <location>
            <begin position="77"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="511"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="539"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="567"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="595"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="624"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="276"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="425"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="463"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR013212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="761" mass="86146" checksum="F663E0386BF4439D" modified="2018-12-05" version="1">DSRWDRRLWFWSPFQNFYFCSFTGRPAGVFLISLESSLSDMNAWREVDFALNCTYIVPDQVSDPSFSLPKAMTSIPRNLTFDYSADNEVTGVFSKEYIPQGTRFGPLQGDLYTKDSVPKEANRKYFWRIYSGGQLQHFIDGYDVQKSNWMRYVNPARCLAEQNLVACQNGRDVYFYTIRPVEPKQELLVWYSQEFAQRLCSHMVASLWKCKNQKPLRVRVSENREAIFSNQKRKRRRCVFFSCGQYGNAAYPETVSQTKLSISVLQLIYDMTHGLPHTSSPTLPPTENHLEEDPEGRGVIPSPQPDRREALLGLNHPYLSDHHPSLPAPHRGLPLHLHGLYGHREGLVSSYPPLYSHSRHLQPPYQFLPPYGPHYPRLILPPYSPPFPSVIPSRGSLRYSSYLGTDGVPYPPIAPPNLLPVSLPYAPSSQGGLKELTPNVSPPRGAPATPELSPPAKPDSYHPSPEQSPSSCEEAMNLSLALNKSSTAPRNGPGHKSLPYPLKKQNGKIKYECNICSKTFGQLSNLKVHLRVHSGERPFQCHLCKKSFTQLAHLQKHHLVHTGEKPHECQVCHKRFSSTSNLKTHLRLHSGEKPYQCKLCNTKFTQYIHLKLHRRLHSSRDRPYRCALCSQTFFHRFSLFVHQRSCCLANSAGPVNVHMKEMVERFDASQEADTLTETASTSQVGEAVEHWLARTLEGEGKEDQKEATVLLKALTAAINAPVTPMVPSAVPVSHHSPTLGFQERASVVHLHRRPTVKTEGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZV2</accession>
    <name>A0A3B5MZV2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DEK-C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MZV2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027807"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028142.1">
        <property type="protein sequence ID" value="ENSXCOP00000027807.1"/>
        <property type="gene ID" value="ENSXCOG00000020733.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017282"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000779">
        <property type="term" value="P:regulation of double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044198">
        <property type="entry name" value="DEK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13468">
        <property type="entry name" value="DEK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13468:SF1">
        <property type="entry name" value="PROTEIN DEK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109715">
        <property type="entry name" value="DEK C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51998">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEK-C" evidence="6">
        <location>
            <begin position="167"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="75"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="242" mass="27101" checksum="121BA03262E519E2" modified="2018-12-05" version="1">EPGPGSQSIPSFSFNGSVSSQSASVKKNLRLFNGFPFLLKLVCSILDLEKKGTHQDLIDRILIFLIAPRNSGKKKLVGGDSLAKKKKSISRASGTKKSKPGSKSKAIVMDSSSDEDDDEKVEAEAGGSDAEEKRSEQEEEEEQTDQSESDEDSSDEDEPLIRMVKKAPTDEQLRKTVESLLKEADLEEMTMKQICLKVFDTYPEHDLSGRKDFIKQTVKTVKCDHFLISLFIFYTVFYFILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZW1</accession>
    <name>A0A3B5MZW1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ANKRD34 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MZW1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027822"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028157.1">
        <property type="protein sequence ID" value="ENSXCOP00000027822.1"/>
        <property type="gene ID" value="ENSXCOG00000020746.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012355"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042637">
        <property type="entry name" value="AN34A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24156">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24156:SF4">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN 34A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="42"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="81"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="212"/>
            <end position="237"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="483"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="496"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="531" mass="57627" checksum="FA9D72F77D3A0A47" modified="2018-12-05" version="1">MGDGGPLQTEGNALLKAVFQGKLRLARLLLEGGAYINEGNERGETPISAACLGNYDDPQTRQRMVRYLLEKGADPNIPDKSGRTALMHACAEQAGREVVTLLLENGADPSLRDYAGASALVHAINRGDRDTLQVLLDACKAKGKEVIIITTDTSPSGTKKTKQYLNSPPSPSIVDKLSPACMSPSEVEIGTASPAGDRTNGQEGIFSFALTSALPLPSTRPPGEKRPPPRKLLKRLNSEPWGLVAPSVLSGVPPDRLDTGLEEEGSSDLSRATAEMNGLSISEPVRTRLSRRHSIETHDPCSPKPIDRSCSEDCTGLSATSWADKVQQHQILYRRNTAPEPQENAGGPGAIRLLVHPKLSRMEHYESDTHLCPESIPGSPDSGRVSVERRRYNASPLSLVTSSSRESLENIPNSVSPITVRRRPPGLLERRGSGTLLLDHISHTRPGFLPPLNINPHRPIPDIRANGKPTSPVHSGHKILVPMAPISPKRGPESKMKKKLMRRHSMQTEQMKQLSTFQEILAEKVVESNGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZY3</accession>
    <name>A0A3B5MZY3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000027857"/>
    <dbReference type="Ensembl" id="ENSXCOT00000028193.1">
        <property type="protein sequence ID" value="ENSXCOP00000027857.1"/>
        <property type="gene ID" value="ENSXCOG00000020776.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156003"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22300">
        <property type="entry name" value="cc_LAMB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000011">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000065">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000101">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000130">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000138">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000145">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000004">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000010">
        <property type="entry name" value="Laminin subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000135">
        <property type="entry name" value="Laminin subunit beta 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000280">
        <property type="entry name" value="Laminin subunit beta 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.300.10:FF:000001">
        <property type="entry name" value="Laminin subunit beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.300.10">
        <property type="entry name" value="Tie2 ligand-binding domain superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056558">
        <property type="entry name" value="LAMB1-4_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013015">
        <property type="entry name" value="Laminin_IV_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF233">
        <property type="entry name" value="LAMININ SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23219">
        <property type="entry name" value="LAMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21199">
        <property type="entry name" value="LAMININ_IV_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00055">
        <property type="entry name" value="Laminin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00136">
        <property type="entry name" value="LamNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51116">
        <property type="entry name" value="LAMININ_IVB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51117">
        <property type="entry name" value="LAMININ_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="8" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 13" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Laminin N-terminal" evidence="18">
        <location>
            <begin position="7"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="248"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="373"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="432"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin IV type B" evidence="17">
        <location>
            <begin position="512"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="720"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="769"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="813"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="972"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="1029"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="1076"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1523"/>
            <end position="1545"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="1200"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1528"/>
            <end position="1545"/>
        </location>
    </feature>
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        <location>
            <begin position="277"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="403"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="415"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="454"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="720"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="722"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="741"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="769"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="788"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="832"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="1001"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="1029"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="1031"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="1050"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="1076"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="1078"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="1097"/>
            <end position="1106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1726" mass="190011" checksum="4B91F2F0D567AADF" modified="2018-12-05" version="1">EFGDVCTEGSCYPATGDLLIGRAHQLLSTSTCGLKRPEPFCIVSHLQQEEKKCFVCDSAAPYDDELANHNHSHRIENVVTTFGPNRLKTWWQSENVENVTVQLNLEAEFHFTHLIMTFKTFRPAAMVIERSADYGKTWQVYRYFAYDCESSFPSVSHGPMKKVDDVICDSRYSDIEPSTEGEVIYRVLDPAFRIEDPYSPRIQLLKITNLRVKFTKLHTLGDNLLDSRMEIKEKYYYAIYDMVVRGNCFCYGHASECAPVEGTGQTMEGMVHGHCMCNHNTKGLNCEQCEGFYHDLPWRPAEGRNTNACKCNCNQHSDSCHFDMAVFMASGNVSGGVCNDCRHNTTGHNCEQCRPFYYQHPEKDLRDPNICQCNCDPVGSLNGGICDGLTDVRVGLIAGQCRCKVNVEGERCEHCKPAYYGLSDEPEGCKCTCSPLGTSPGGNPCDSETGSCFCKRLVIGRDCDQCHWGLSNDMDGCRPCDCDLGGAVHNQCVCRDHMFGRRCDQVESGFYFVALDHYVYGVTMVPRPHPHDRSPTWTGVGLVNVPEGAFLDFSVDNIPHSMEYDILIRYEPQLPDQWEEVLMIVMRPGPITADSRCVNTVPDDDNQMISLHPSSYVVLPRAVCFESGLNYTIRLSLPLYSALSEVHSPYTLIDSIVLMPHCKNLEIFTSSVGGDSSAWETFQRYRCLENSQSVIKTPMTDICRNFIFSISALLQQGAKCQCDPQGSLSTVCDPSGGQCQCRPNVAGRNCDRCAPATFQFGPSGCKCACDPQGSQSPICDQLSGQCVCVAGAYGRQCDRCLPGHWGFPACRPCSCNGHTESCDAATGRCTACRDHTTGHSCDCLDGYYGDPVLGSGDHCRPCLCPDGPNSLRQFAGSCYRSDTSEQVVCVCNPGFKARCDQCSPGYYGNPEQAGGQCLPCQCNNNIDMLDPESCDARSGECLRCLYHSEGPSCQNCKLGYYGNALVQDCRECVCNQLGSDESSCQSSTDCHCDRSSGQCHCRPNAIGQHCDRCAPDTWNMASGAGCQSCDCNPEHSYGTTCNEISGQCSCQPGFGGKTCNECRELFWGDPEVQCHCDCDPRGTLEPQCNKVSGHCVCVEGVAGSRCNACARGFHGTFPDCHRCHQCFSEWDNVIGQLTNQTHRLVNKVSSIKASGISGPYRKSIESMERSASDIQSILDQDPASQPLTEVQNLLQQADLMAALSQTMNNTEETLDLVAEQDSGIKTQLDSVTSDAQKLERTVQELLDQVEFIKNSNIRATDSVTKYFLQSQAAEARANASTINAGNPVESSAALRQLTEDKMNQTREEFLERQSEHAQKLDNLAGEMETLDLSVISYKTCGSPSSGQDSCWSSPCGGLGCVDPEGQPKCGGEGCDGAVTAANSILLKTQEAEQEIISAMAEVEKLSKMVLEVKMQADEAKLSAQNVLMKTNRTKHKVDQSNDELRSLIRQIKDFLTDAADLESIELVANEVLAMQMPTTPAQLQNLTDEIRHKVGELGHVEAILQQSADDIQRAETLLDQARQAKQATDTKDSAEKVKQALEEAQRAHTAASSAIQQAASDIQTTAKLLSSVETETADAESKLDNATQRLQRLEQDVKLLADRSANVTQRTKLANQEAADIGRIAEEVKKEFESEVKQKYSTVEQLIDQKAGGVADAKKRAESLQNQAKQLLLQASDKLQLLKLEKSYDDNQRTLELKAEQLVEMEAAVKKLLQEISHKVTLYSTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N035</accession>
    <name>A0A3B5N035_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">STX17-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5N035"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026335"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026654.1">
        <property type="protein sequence ID" value="ENSXCOP00000026335.1"/>
        <property type="gene ID" value="ENSXCOG00000019667.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01090000260119"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059001">
        <property type="entry name" value="STX17_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26585">
        <property type="entry name" value="STX17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="STX17-like N-terminal" evidence="3">
        <location>
            <begin position="29"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="232" mass="24972" checksum="0D2FC8301D54AF70" modified="2018-12-05" version="1">VFNDCFSVISRHMLPPVQSLLNPLTCESCTVLQQLRANMREMEQLCSRVRSEDAEALEELVKPVRDRASAAAQDFLLLHSNPPPPVPAAPPDTRPSISRSSSCHDLEEEDEEPLSDLQIQLRLPEIPADQNASESWDNLEEVPYLFFMSLQAAGYKLAVLPVAGALLGGVLGGPLGLLAGFKAAGVAAAVGGGALGFAGGSLVRKHRRAQVDQQMSRLTAPPAEELEENKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N103</accession>
    <name>A0A3B5N103_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Granulins domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the granulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N103"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027055"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027388.1">
        <property type="protein sequence ID" value="ENSXCOP00000027055.1"/>
        <property type="gene ID" value="ENSXCOG00000020207.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00470000042293"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.160">
        <property type="entry name" value="Granulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000118">
        <property type="entry name" value="Granulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039036">
        <property type="entry name" value="Granulin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037277">
        <property type="entry name" value="Granulin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12274">
        <property type="entry name" value="GRANULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12274:SF7">
        <property type="entry name" value="GRANULINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00396">
        <property type="entry name" value="Granulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00277">
        <property type="entry name" value="GRAN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57277">
        <property type="entry name" value="Granulin repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00799">
        <property type="entry name" value="GRANULINS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017211679" description="Granulins domain-containing protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Granulins" evidence="6">
        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="Granulins" evidence="6">
        <location>
            <begin position="155"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="277" mass="30267" checksum="D156E408395F1FE2" modified="2018-12-05" version="1">MLRMTVWLCVGVFLWGFASCSIRCPDGSSCSDMETCCQTAGGYNCCPYPKAVCCSDLAHCCPSGYLCDLATQMCVKQYQPWITIPIVRKVAAEVSSTPDLSLTPFEEVKQNNSPEQLKSSGVYCDSYTACPDGTTCCKHPKSGWFCCPLSPAKCCLDGFHCCPYGYDCDHTYTQCVRERLEDLFTYKKSLTSVPASLLSVSENKTLTALTEASANSFNQGVIRCDDKFYCPGGSSCCKGPKDKWNCCPYPLGVCCADGKHCCEYGYQCNPSSSQCTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N124</accession>
    <name>A0A3B5N124_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 16">Amyloid-beta A4 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">APP</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Functional neuronal receptor which couples to intracellular signaling pathway through the GTP-binding protein G(O).</text>
    </comment>
    <comment type="function">
        <text evidence="16">Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cell membrane</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15 16">Belongs to the APP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5N124"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027115"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027448.1">
        <property type="protein sequence ID" value="ENSXCOP00000027115.1"/>
        <property type="gene ID" value="ENSXCOG00000020241.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063252"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005798">
        <property type="term" value="C:Golgi-associated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099503">
        <property type="term" value="C:secretory vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030546">
        <property type="term" value="F:signaling receptor activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051246">
        <property type="term" value="P:regulation of protein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.140:FF:000001">
        <property type="entry name" value="Amyloid beta (A4) protein b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.570.10:FF:000001">
        <property type="entry name" value="Amyloid beta A4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.770:FF:000001">
        <property type="entry name" value="Amyloid beta A4 protein-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.770">
        <property type="entry name" value="Amyloid precursor protein, E2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.140">
        <property type="entry name" value="Amyloidogenic glycoprotein, copper-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.570.10">
        <property type="entry name" value="Amyloidogenic glycoprotein, heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR036669">
        <property type="entry name" value="Amyloid_Cu-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008155">
        <property type="entry name" value="Amyloid_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013803">
        <property type="entry name" value="Amyloid_glyco_Abeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011178">
        <property type="entry name" value="Amyloid_glyco_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024329">
        <property type="entry name" value="Amyloid_glyco_E2_domain"/>
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    <dbReference type="InterPro" id="IPR008154">
        <property type="entry name" value="Amyloid_glyco_extra"/>
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    <dbReference type="InterPro" id="IPR015849">
        <property type="entry name" value="Amyloid_glyco_heparin-bd"/>
    </dbReference>
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        <property type="entry name" value="Amyloid_glyco_heparin-bd_sf"/>
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        <property type="entry name" value="Amyloid_glyco_intracell_CS"/>
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        <property type="entry name" value="APP_amyloid_C"/>
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        <property type="entry name" value="APP_CUBD_CS"/>
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        <property type="entry name" value="E2_sf"/>
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    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR23103">
        <property type="entry name" value="ALZHEIMER'S DISEASE BETA-AMYLOID RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23103:SF7">
        <property type="entry name" value="AMYLOID-BETA PRECURSOR PROTEIN"/>
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        <property type="entry name" value="APP_amyloid"/>
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        <property type="entry name" value="APP_Cu_bd"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Beta-APP"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00203">
        <property type="entry name" value="AMYLOIDA4"/>
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    <dbReference type="PRINTS" id="PR00204">
        <property type="entry name" value="BETAAMYLOID"/>
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        <property type="entry name" value="A4_EXTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56491">
        <property type="entry name" value="A heparin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89811">
        <property type="entry name" value="Amyloid beta a4 protein copper binding domain (domain 2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109843">
        <property type="entry name" value="CAPPD, an extracellular domain of amyloid beta A4 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="APP_CUBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51869">
        <property type="entry name" value="APP_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="APP_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00320">
        <property type="entry name" value="APP_INTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16" id="KW-0034">Amyloid</keyword>
    <keyword evidence="16" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0186">Copper</keyword>
    <keyword evidence="12 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="627"/>
            <end position="649"/>
        </location>
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        <location>
            <begin position="19"/>
            <end position="180"/>
        </location>
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            <begin position="19"/>
            <end position="114"/>
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        <location>
            <begin position="122"/>
            <end position="180"/>
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            <begin position="184"/>
            <end position="261"/>
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            <begin position="332"/>
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        <location>
            <begin position="488"/>
            <end position="515"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="215"/>
            <end position="261"/>
        </location>
    </feature>
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        <location>
            <begin position="64"/>
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    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="89"/>
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    </feature>
    <feature type="disulfide bond" evidence="15">
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            <begin position="124"/>
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    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="135"/>
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    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="149"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00016844"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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            <dbReference type="ARBA" id="ARBA00022900"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023008"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023087"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00053261"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="696" mass="79461" checksum="C666513CF353B5B0" modified="2018-12-05" version="1">MTFSLYALPQVPADVSMGLLAEPQVAMFCGKLNMHINVQSGKWEPDPSGRKSCIGTKEGILEYCQEVYPELQITNVVEANQPVSIQNWCKKGRNQCRSHMHIVVPYRCLVGEFVSDALLVPDKCKFLHQERMDKCESHLHWHTVAKENCGDRTMNLHDYGMLLPCGIDRFRGVEFVCCPSEAERGVDSVEQDADDSDVWWGGVETDYADNRWDEDKPEEEGDDDEEEDEEEEDDQLDNDQDGDGEEDEEAVDEEDDDDEDDGVIIDALDDADEPTTNVAMTTTTTTTTESVEEVVKVPTAKPSSPDAVDNYLETPADENEHAHFQKAKESLEAKHRERMSQVMREWEEAEREAKNLPRADKKAVIHRFQEKVEALEQEAASERQQLVETHMARVEALLNDRRRLALESYLTALQQDPPRPRHVFSLLKKYVRAEQKDRQHTLKHFEHIRMVDPKKAAQIRPQVLTHLRVIEERMNQSLGLLYKVPGVADDIQDQVELLQREQAEMAQQLANLQTDARVSYGNDALMPDQEFGDGQTDPLPQEDTLGGVGFIHPESFNQQNTENQGAYLKKKKEIKLSYRFFLLVTGVKMDAVPEMRMETEDRQSTEYEVHHQKLVFFAEDVSSNKGAIIGLMVGGVVIATVIVITLVMLRKKQYTSIHHGVIEVDAAVTPEERHLSKMQQNGYENPTYKFFEQMQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KJR8</accession>
    <name>A0A3B5KJR8_9TELE</name>
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        <recommendedName>
            <fullName evidence="11">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KJR8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000413"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000421.1">
        <property type="protein sequence ID" value="ENSXCOP00000000413.1"/>
        <property type="gene ID" value="ENSXCOG00000000341.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165754"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd21998">
        <property type="entry name" value="HMG-box_UBF1_rpt1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21999">
        <property type="entry name" value="HMG-box_UBF1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22000">
        <property type="entry name" value="HMG-box_UBF1_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22002">
        <property type="entry name" value="HMG-box_UBF1_rpt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22003">
        <property type="entry name" value="HMG-box_UBF1_rpt6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029215">
        <property type="entry name" value="HMG_box_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051762">
        <property type="entry name" value="UBF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46318:SF5">
        <property type="entry name" value="NUCLEOLAR TRANSCRIPTION FACTOR 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46318">
        <property type="entry name" value="UPSTREAM BINDING TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09011">
        <property type="entry name" value="HMG_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14887">
        <property type="entry name" value="HMG_box_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="11">
        <location>
            <begin position="94"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="11">
        <location>
            <begin position="178"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="11">
        <location>
            <begin position="277"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="11">
        <location>
            <begin position="411"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="11">
        <location>
            <begin position="494"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="8">
        <location>
            <begin position="94"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="8">
        <location>
            <begin position="178"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="8">
        <location>
            <begin position="277"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="8">
        <location>
            <begin position="411"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="8">
        <location>
            <begin position="494"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="367"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="475"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="570"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="386"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="628"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="658" mass="76769" checksum="D72C49253393918B" modified="2018-12-05" version="1">ADAWSQEDCLTLLERIRGLLPDGDAMKYKTTESHFDWDKVGFGRFTGDMCRQKWQKVSSEVRKYRTMTELIVDAIEFVKNPYKGKKLKTHPDFPKKPLTPYFRFFMEKRAKYAKIHPEMSNLDLTKILSKKYKELPDKKKQKYITEFQREKEEFEKNMARFREEHPDLMEERKKSDLPEKPKTPQQLWYNHEKKAYMKLHPEVSQKELKEALRRQWSQLSDKRRLKWISKALELQKDYEDSMRAYHEAHPDSNSEEHVRSVLTKAERQLKDKFDGRPTKPPNGYSLYCAELMVNMKDVPSTERMVLCSKQWKMLTQKEKDMFQKRCEQKKKQYDVDLQRFLESLPEEERDRVLTEEKMGGAKLNVGVVPSPLRAKSPSRGREAEPEAWPAAAGGASAPRERRDARKTAKLPETPKTAEETWQHSVTGDYLAKYRSDRRKAQAAMEAAWRSMEKKEKIPWIKKAAEDQKRYERELLEMRAPPVGQRKPKFDGEPKKPPRSGYQMFSQELLTNGELNHFSLKERMVEIGKRWQKLSQAQKDKYKKQVEEQQVEYKAHLEAWVKSLSPQDRAVYKEFSSSKRRSSSSKATASPVAKVRVTAKGKVGGARAVTPGVTVGKRAMAYRAKDMSDSDEEEEKSGSSDSDEDDETSGSSDSEDEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KPU7</accession>
    <name>A0A3B5KPU7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">2',5'-phosphodiesterase 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Mitochondrial deadenylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CCR4/nocturin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KPU7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000487"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000495.1">
        <property type="protein sequence ID" value="ENSXCOP00000000487.1"/>
        <property type="gene ID" value="ENSXCOG00000000407.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157205"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000018">
        <property type="entry name" value="2',5'-phosphodiesterase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048821">
        <property type="entry name" value="PDE12-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF37">
        <property type="entry name" value="2',5'-PHOSPHODIESTERASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21171">
        <property type="entry name" value="PDE12-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="2',5'-phosphodiesterase 12-like N-terminal" evidence="16">
        <location>
            <begin position="144"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="15">
        <location>
            <begin position="272"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00072755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00083541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF21171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="585" mass="64220" checksum="BC7E5189DAE33B57" modified="2018-12-05" version="1">DMYPPNSTFLTSRVVHFLKTIFPDDSGVFKRDNAAVVRCLPGEPKLTISFCLEDSQKHMLRDQNEALGKVLTRISNTIAKNQAKAKKSKKKLDQQPSEAAETAVVKLFFKGDEVPDTLPNCEAWQDGAVLQVGEVQYNVQRNAPTFTTAELPVSLMAGFPVCPKLQVEFGNLQDCEYMWFKENSTNTSPKDSTAPEGGWTQVGCERVLVPSNQDIGCRLKLRCTPKDGSRTGQAKELVSVATVEAGPGVCTFDNRHSYTLRAAAWPALRVVSYNILADVYAQTDLSKTVLYPYCAPYALQLDYRQNLVKKELAGYNADIICLQEVDKGVFVDSLTPALDAFGLDGVFRLKEKQHEGLATFYCRSKFRLLSRHDIMLSEALTSDPLHSELLEKLSANNGLKDRILQRSTALQVQLETGSSCSQKVCVANTHLYWHPKGGNVRLVQMGVALQHLSQVIADVAPGAPLVFCGDLNSSPGSGNRSKLLSEAAVPPQHADWSSSGPEESCCMPLSSTFPPLQSACGQPAYTNYVGGFHGCLDYIFIQPQSMQVEQVVPLPSHQEVTTHKALPSVAHPSDHIALVCDLLWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTD9</accession>
    <name>A0A3B5KTD9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTD9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000892.1">
        <property type="protein sequence ID" value="ENSXCOP00000000881.1"/>
        <property type="gene ID" value="ENSXCOG00000000741.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="78" mass="9415" checksum="EBA8B26A008F757F" modified="2018-12-05" version="1">MNFVFQHDIDSKRLKKSKQKKSKVLQWPCQKKAVWENMSKSRCKGDIKELNLTYLCNFHLLKRVPQNNRSTFRLLTNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KTW8</accession>
    <name>A0A3B5KTW8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitotic spindle assembly checkpoint protein MAD2B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Mitotic arrest deficient 2-like protein 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KTW8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003563"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003602.1">
        <property type="protein sequence ID" value="ENSXCOP00000003563.1"/>
        <property type="gene ID" value="ENSXCOG00000002810.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153395"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.900.10:FF:000003">
        <property type="entry name" value="Mitotic spindle assembly checkpoint protein MAD2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045091">
        <property type="entry name" value="Mad2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842:SF10">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HORMA" evidence="6">
        <location>
            <begin position="13"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="200" mass="22931" checksum="6EA247C3DF585A71" modified="2018-12-05" version="1">MTTLTRQDLNFGQVVADILCEFLEVAIHLILYVREVYPSGIFQKRKKYNIPVQMSCHPELNQYIQDTLHCVKPLIEKNDAEKVVVVIMDKEHHPVERFVFEISQPPLLSISSDTLLSHVEQLLRAFILKISVCDAVLNNNPPGCSFTVLVHTRDAATRNMEKVQVIKDFPWIVADEQEVHMKEPRLIPLKSMTSDIVKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KU10</accession>
    <name>A0A3B5KU10_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KU10"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001460"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001483.1">
        <property type="protein sequence ID" value="ENSXCOP00000001460.1"/>
        <property type="gene ID" value="ENSXCOG00000001177.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234530"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="Ig_V-set"/>
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        <property type="entry name" value="TRG_var/const_region"/>
    </dbReference>
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        <property type="entry name" value="T CELL RECEPTOR GAMMA VARIABLE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19256">
        <property type="entry name" value="T-CELL RECEPTOR GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
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    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017274854" description="Ig-like domain-containing protein" evidence="5">
        <location>
            <begin position="32"/>
            <end position="311"/>
        </location>
    </feature>
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        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
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            <begin position="27"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="141"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="311" mass="34881" checksum="2495D4F6D170E444" modified="2018-12-05" version="1">LLRVHLLQQSNILLFMLFLNVSILLFPDIDASVPVVTAEVGQSVTLTCEFNMKYQSNTWIYWYKQSAGDTLNLIVMQQRTTTSTYGSQFDASRFNVANTGDSSILKIFKTVQQDEGMYHCFTIYNFTGYKRTSNYTVVQQPTTSGSTRPTGSETLQCSVLSDSENATCSGEPSVFWFRARSEKSFPDMIYIDGKRSENCDKTSDSQKKCFYNLSKNISSSDAKTLSCAVATCGQILFANRLNLKNVTIICLVISVICNIVFIFFRIQRSTEAGPDLNYAALHLSDGKRSRGKKKRELMTEESVYSQVKGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KUI5</accession>
    <name>A0A3B5KUI5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LYR motif-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LYRM2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Atherinomorphae</taxon>
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            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
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    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in efficient integration of the N-module into mitochondrial respiratory chain complex I.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KUI5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001295.1">
        <property type="protein sequence ID" value="ENSXCOP00000001276.1"/>
        <property type="gene ID" value="ENSXCOG00000001027.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002957"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd20262">
        <property type="entry name" value="Complex1_LYR_LYRM2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Complex1_LYR_dom"/>
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        <property type="entry name" value="LYR MOTIF-CONTAINING PROTEIN 2"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR13675:SF0">
        <property type="entry name" value="LYR MOTIF-CONTAINING PROTEIN 2"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="7">
        <location>
            <begin position="25"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00026235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="93" mass="10793" checksum="6259124050539AFC" modified="2018-12-05" version="1">VDGTVHMGSRLPPAALSLKQFLQRQKVLGVYRSMLRTIRQVPDEADRKYLRGWAREEFKRNKNATNQDAIRMMITQATNHLEELQKSLLLARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KV86</accession>
    <name>A0A3B5KV86_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KV86"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001521"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001546.1">
        <property type="protein sequence ID" value="ENSXCOP00000001521.1"/>
        <property type="gene ID" value="ENSXCOG00000001229.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00099">
        <property type="entry name" value="IgV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
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    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052051">
        <property type="entry name" value="TCR_complex_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433:SF133">
        <property type="entry name" value="IMMUNE-TYPE RECEPTOR 5 PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19433">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="32"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="125"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="262" mass="30219" checksum="51AF71032DCC5841" modified="2018-12-05" version="1">FVHLFIILSLLIVMIMLSWFKFKFEVKTVTVGQNVTLSCPHQTSALYRETLYWIRLVSGNWPEFLGATFNFMDDDVSKISHIQTKQEQGKFLLYVNKAKRNDNGLYYCIKIRHNFLLIVRLKHMPPSGLVYPETGETLQCSVLSDSERKSCPADNRVYWFKAGSDHSHPSLLYVQENSGEECESNPEAPATQKCFYNFSETSDAGTYHCAVAACGQILFGNEDKRHYEGKSLRFSLYQRICFNYGDIAYIQQITVLINRFHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KWD4</accession>
    <name>A0A3B5KWD4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KWD4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004481.1">
        <property type="protein sequence ID" value="ENSXCOP00000004428.1"/>
        <property type="gene ID" value="ENSXCOG00000003497.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000271558"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="70" mass="8113" checksum="64321AC192E7E923" modified="2018-12-05" version="1">YSLCKLPCVQSVIMCYNLEIVYFCYFHSVLTLKCPKGRKYYSATARQANPPHRGVFSSFVQTYIKVHSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KWM3</accession>
    <name>A0A3B5KWM3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">FERM domain containing kindlin 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">FERMT3</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the kindlin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KWM3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002026"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002057.1">
        <property type="protein sequence ID" value="ENSXCOP00000002026.1"/>
        <property type="gene ID" value="ENSXCOG00000001625.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013444"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030055">
        <property type="term" value="C:cell-substrate junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033622">
        <property type="term" value="P:integrin activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007159">
        <property type="term" value="P:leukocyte cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070527">
        <property type="term" value="P:platelet aggregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033632">
        <property type="term" value="P:regulation of cell-cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13205">
        <property type="entry name" value="FERM_C_fermitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17185">
        <property type="entry name" value="FERM_F1_KIND3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01237">
        <property type="entry name" value="PH_fermitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000037">
        <property type="entry name" value="Fermitin family homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037843">
        <property type="entry name" value="Kindlin/fermitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040790">
        <property type="entry name" value="Kindlin_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037837">
        <property type="entry name" value="PH_Kindlin/fermitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16160">
        <property type="entry name" value="FERMITIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16160:SF1">
        <property type="entry name" value="FERMITIN FAMILY HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18124">
        <property type="entry name" value="Kindlin_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="346"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="310"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="656" mass="75067" checksum="94F75323EA6E2C7F" modified="2018-12-05" version="1">FLHTLCSCLSQSMAAWDLSVKVEDLGPDAPSVTISVASDLHVGGVILKLVEKTQMQRDWSDHALWWEQKHQWLLRTAWTLEKYGIHADARLLFMPQHKPLKLGLPNGKTVMLRACFSNPVFQTVMGICRMLNIRHPEELSLLRPVEEKKKKKDKDLTEEIYDLTEVPLSSVSRPCLYNGMPAHFADSEQSEGVYKMLSVTQPPPAPEVIAKQYRPASVVDKSHIHGRWLDSSRCLMQQNIQENDRLWLRFKYYSFYDIEPKYDVVRLTQLYEQARWAILLEDIDCTEEEMMLFGALQYHINKVALSEPQMMSSNAALDDLESALQSLEVKMEGESSSASDLLVAWILILNPMLVHFRPKRLTLKGYKQYWFKFQDTSISYFKSKEESIGEPIQQINLKGCEVAPDVNVAAQKFLIRLLIPAPEGMNEVYLRCENEQQYAQWMAGCRLASKGKSLADSSFQSEIQSIRSFLAMQKTNSGSSNNSAASDESINTHSLVSPRYHKKYKPKQLTPRILDAYQNVAQLSLTDALMRFLQIWQALPDFGLSYVVVRFKGSRKDEVLGIAPNRLIRIDLGVGDVVKTWRYNNMKQWNVNWDIKQMAIEFEGNVNIAFGCVTADCKIVHEFIGGYIFMSTRSREKSDTLNEELFHKLTGGHEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXH7</accession>
    <name>A0A3B5KXH7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histidine decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 10 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.22" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXH7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000264"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000271.1">
        <property type="protein sequence ID" value="ENSXCOP00000000264.1"/>
        <property type="gene ID" value="ENSXCOG00000000194.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157938"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004398">
        <property type="term" value="F:histidine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042423">
        <property type="term" value="P:catecholamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001694">
        <property type="term" value="P:histamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006548">
        <property type="term" value="P:L-histidine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010977">
        <property type="entry name" value="Aromatic_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999">
        <property type="entry name" value="GROUP II PYRIDOXAL-5-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11999:SF68">
        <property type="entry name" value="HISTIDINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00800">
        <property type="entry name" value="YHDCRBOXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0127">Catecholamine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 10 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="567" mass="63934" checksum="2A6E1DBBEA1DCB62" modified="2018-12-05" version="1">NVVLQMHFSRKCKNAPSHDSRAVSFSVNQHRSVTENMFPETQKPEIMVPGARAPAPDYKHFSTVSEGTLVALLAARKDKILQLKTELDQEVDDSVLNSTLVAYASDQAHSSVEKAGLIALVKIRFLPADEKLSLRGETLKQAIQEDRRRGLVPFMLCSTLGTTGVCAFDKLSELGPICEEEGLWLHVDAAYAGSAYFCPELRWSLEGIEFAESFVFNPSKWMMVHFDCTAFWVKNKHKLQQTFSVDPVYLRHENSRAATDFMHWQIPLSRRFRSLKLWFVMRSFGLKNLQAHVRHGIEMAKLLESHIRSDPNFEVPAERHLGLVVFCLKGGNALTQELLRRLTRSGTMYLIPAEIHTKRIIRFTVTSQFTTADDILKDWAFISKTATTLLAEKHGFNKADQLRYGEPKAAGKEENPDPHLDAMADEKEDVVTQLEKAQIDLWIDKAWNRPRRPMRSLSCSSEPLPYWALFSFDSTESGPVSTLAEMPSSLPRKQVLKKLTKFYSVPSFCNQWAQCSRHPVNCPLKVAHASQKPLSSSCRRVNCMLSSPAANMPSTSPLESANGAEPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXJ8</accession>
    <name>A0A3B5KXJ8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXJ8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003277"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003315.1">
        <property type="protein sequence ID" value="ENSXCOP00000003277.1"/>
        <property type="gene ID" value="ENSXCOG00000002585.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="113" mass="12906" checksum="0E99F8D66CFCD956" modified="2018-12-05" version="1">AIYTRLEYSERSNSYSEISSKELEVNFIEVSAFVASCSKTCVSADRMNKYLTISMSSLTYHVLILFSCILPGFFEDAKSDFQQALKLDANFEDAKMSLQQTLLDQQEKLNRGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KY02</accession>
    <name>A0A3B5KY02_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RecA family profile 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KY02"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003437"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003475.1">
        <property type="protein sequence ID" value="ENSXCOP00000003437.1"/>
        <property type="gene ID" value="ENSXCOG00000002710.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000020445"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033063">
        <property type="term" value="C:Rad51B-Rad51C-Rad51D-XRCC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042148">
        <property type="term" value="P:DNA strand invasion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19490">
        <property type="entry name" value="XRCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030547">
        <property type="entry name" value="XRCC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46644">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46644:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="2">
        <location>
            <begin position="28"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="304" mass="33316" checksum="198ACBE6309B8937" modified="2018-12-05" version="1">KSKRSRANDQEEEEGRTEMSESGTQLLARLEARRCLKDIEPQLFADDGGPDRGEVVEVYGPEGTGKTELLYHLLCRCVLPEAAGGLEVDVVFVDTDYSLDVFRLASVLDSRLNGSAGSDDAVLRSCLSRLLVVRCSSSSELLLTLHFLETTLSSRPKAALLLIDSISAFYWSDRSEGGASVAKQEEKLSRCSELLGRLLRDYRITIFATCHPIRRTSSGPPSSSSEADRSHLCRAWQRLVTHRLVCSKQEAEHNPANGDQGSGEQRNRQVFTVHCSSWTGTKGSRVSSFRVTDGGVEDGSESGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZ10</accession>
    <name>A0A3B5KZ10_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDE1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZ10"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003280"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003318.1">
        <property type="protein sequence ID" value="ENSXCOP00000003280.1"/>
        <property type="gene ID" value="ENSXCOG00000002587.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234623"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005016">
        <property type="entry name" value="TDE1/TMS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383">
        <property type="entry name" value="SERINE INCORPORATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383:SF51">
        <property type="entry name" value="SERINE INCORPORATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03348">
        <property type="entry name" value="Serinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="116" mass="12860" checksum="585290B133E4D68D" modified="2018-12-05" version="1">MGALQGIFTFTSCMCSNATCPVCGCCPSSRNSTVTRIIYASILLLGTIVACIMLSPGMEQQLRRVRINYAITCEMLVGYKAVYRFCFGMSMWFLSFSIFTLNIKTSRDPRASIHNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZF0</accession>
    <name>A0A3B5KZF0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Klotho beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027865027.1">
        <property type="nucleotide sequence ID" value="XM_028009226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZF0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003932"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003976.1">
        <property type="protein sequence ID" value="ENSXCOP00000003932.1"/>
        <property type="gene ID" value="ENSXCOG00000003079.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114139336"/>
    <dbReference type="GeneTree" id="ENSGT00940000157489"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000042">
        <property type="entry name" value="Klotho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000062">
        <property type="entry name" value="Klotho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF68">
        <property type="entry name" value="BETA-KLOTHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017332242" description="Klotho beta" evidence="10">
        <location>
            <begin position="28"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="992"/>
            <end position="1015"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1050" mass="119456" checksum="2553C96221A9A0B3" modified="2018-12-05" version="1">MLNHPSCHTHLLVSLLLLTFPWDRAVCSPGEGRSIWQRPKPSPISKDQSFLQDTFPPGFIWGSGTSAFQNEGAWNQHGKGVSIWDHFTHSSTSDYFPRETADVASDSYNCWEEDVAALGYLGVRSYTFSLSWPRLFPEGNATGEPNAAAVDHYSRLIERLLEKKIEPIVTLYHWDLPQVFQDQYGGWKNNTLVEHFEQYATFCFSTFGSRVKYWLTMHNPYLVAVQGYGTGVHAPGEKGGVSSSLTVAHNLIRAHAKAWQTYNTHFRPTQRGKVSIVLGSHWVEPHRGQPTAANVALCQQSMEAILGWFADPIFGAGDYPVSLKIKYADLMPRFTPEEKLWVQKTADFFALSFGPNNFRLGQNLLHNGQSITPDLRRLLGWIKLEYGNLRVLVAEGGWFSDASVGREDTVAIYLMKSFINQVLQAIKFDGVQVFGYSAWSLLDGFEWNYGYSVRRGLFYVDFNQPNRTRVPKTTAQYFKQIVNDNGFPADENSSTDVKGHFPCKFHWGIADSTLQVRFHPFSPQFSDPHVYSWNLTGDGSLQPLPGVKLNTRTAQCTDYLAIRNHLRLFASTGASHYRFALNWSLILPEGSSSNVNTEALRYYRCVLSEIKKLNLEAMVILYHPTHRAPNLGLPGPLHASGGWLNYTTVGAFQEYVALCYQELGTWVHYWITINEPNRLIDVYSNVTERHQATHNLLMAHAKAWRLHEREYFGKQRSLVSLALHADWASPGNPFLESHIVATERFLLFELGFFLDPLLETRGEKKKGQHPYEMRESLGERARVFGLPKTPLPHFTTTERQELNGALSFIALNHFTTRLVSPNPHKQDILQKKPAPEHDCVTFSDPTWLSSNLGQALVPWGLRKMLKWVNQRYGRTLPIIVTASGIDDQASVEDNLRQYYLRSYLQEALKAYHLDGVNLQGFYVWKLQDRHAPLFGLFTSTQHQSKAKASVALYRKIIANSGSSNNSTTQLCRLTEQQEKCLTCEWLLKNKPMLVFGGCLLITSAMLAALIIFIVISKRNKKHDRGRKKRRENRRRGNKATAYCLCPDVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L007</accession>
    <name>A0A3B5L007_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acetylserotonin O-methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Hydroxyindole O-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine).</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Aromatic compound metabolism; melatonin biosynthesis; melatonin from serotonin: step 1/2.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.4" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L007"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005668"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005734.1">
        <property type="protein sequence ID" value="ENSXCOP00000005668.1"/>
        <property type="gene ID" value="ENSXCOG00000004413.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166228"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017096">
        <property type="term" value="F:acetylserotonin O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030187">
        <property type="term" value="P:melatonin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000358">
        <property type="entry name" value="Acetylserotonin O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF2">
        <property type="entry name" value="O-METHYLTRANSFERASE CICE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0471">Melatonin biosynthesis</keyword>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="11">
        <location>
            <begin position="17"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="10">
        <location>
            <begin position="121"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="242" mass="26986" checksum="7786E8A6D3E70080" modified="2018-12-05" version="1">MAEHLSQSELDYPFKLLEYFNGFRVSKMIFSACELGVFDLLLKSQDALSAQHVAQELGTSVDGMERLLDALVGIEILEVERADGTALYSSTDVANLYLAKGSAKSLHDMIIYRSQTVYPLWNNLADAIREGRNQYEKTFGLPSAHFFQAIYRSEEEMLKFMGIVSCHWVLDGHDVVTAFDLSRFQKVVDLGGCTGALANEMARAYPASSVTVFDLPEVVEAAQKHFKQEDSGVTFQSGKPEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L0I4</accession>
    <name>A0A3B5L0I4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L0I4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001319"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001342.1">
        <property type="protein sequence ID" value="ENSXCOP00000001319.1"/>
        <property type="gene ID" value="ENSXCOG00000001078.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004215"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14132">
        <property type="entry name" value="STKc_CK2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000059">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000088">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF28">
        <property type="entry name" value="CASEIN KINASE 2 ALPHA 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="39"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="336" mass="40100" checksum="C719E5725E61F106" modified="2018-12-05" version="1">MSGPVPSRSRVYPDVNTQRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIISLLDIVKDPVSRTPALVFEHVNNTDFKQLYQTLSDFDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPNQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKRWERFVHSENQHLVSTEALDFLDKLLRYDHQARLTAREAMDHPYFCMIASSLVLFFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L134</accession>
    <name>A0A3B5L134_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ADP-dependent glucokinase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027887641.1">
        <property type="nucleotide sequence ID" value="XM_028031840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L134"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004507"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004563.1">
        <property type="protein sequence ID" value="ENSXCOP00000004507.1"/>
        <property type="gene ID" value="ENSXCOG00000003561.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114153348"/>
    <dbReference type="KEGG" id="xco:114153348"/>
    <dbReference type="CTD" id="83440"/>
    <dbReference type="GeneTree" id="ENSGT00390000017953"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043843">
        <property type="term" value="F:ADP-specific glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01938">
        <property type="entry name" value="ADPGK_ADPPFK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007666">
        <property type="entry name" value="ADP_PFK/GK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21208">
        <property type="entry name" value="ADP-DEPENDENT GLUCOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21208:SF0">
        <property type="entry name" value="ADP-DEPENDENT GLUCOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04587">
        <property type="entry name" value="ADP_PFK_GK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51255">
        <property type="entry name" value="ADPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017444440" description="ADP-dependent glucokinase" evidence="6">
        <location>
            <begin position="21"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="495" mass="54442" checksum="56B583F87B6337E1" modified="2018-12-05" version="1">MWRKASAALLLALAVGYFYGGRPELPEQILQYIGLPNLQTPSPSNEGLERAISAAWEALIALPSRQWSKVAVGVNACVDVVVSGVGLLQALAVDPGAGRDHAVLRSKDDLRETFVHYMERGAAAERFFSDKEVFQRIARAAAEYPSAKLYVGGNAALIGQKFATYPDLMVLLCGPVGPKLHEMLDEQIVVPPESLQETDEFHLILEYKAGEQWGSTRAPQANRFIFSHDVSNGEMSSLETFVASLEEFQPELVVLSGFHMMEGQGRELWGERLKEAVATIADIPRGIPIHLELASMTDKDFMSRIMQEQVLPIVSSVGLNEQELLFLSQAGDGPHAALPAWKGVPDVGRVGDILYWVLERHGRTDPSSESDLTRIHFHTLAYHILVTVDGYWGNQAAAVMAGARVASSQACGLRTVDVSKVELKAPLEFLTSHLEPRERLSLKPEEPVAVWRRGNITFHLTPVLVCKRPLRTVGLGDAISAEGLVYSELTRQHPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L370</accession>
    <name>A0A3B5L370_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Sorting nexin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the sorting nexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027880419.1">
        <property type="nucleotide sequence ID" value="XM_028024618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L370"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006763"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006846.1">
        <property type="protein sequence ID" value="ENSXCOP00000006763.1"/>
        <property type="gene ID" value="ENSXCOG00000005233.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114149061"/>
    <dbReference type="KEGG" id="xco:114149061"/>
    <dbReference type="GeneTree" id="ENSGT00940000157724"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036089">
        <property type="term" value="P:cleavage furrow formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000033">
        <property type="entry name" value="Sorting nexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000116">
        <property type="entry name" value="Sorting nexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000004">
        <property type="entry name" value="Sorting nexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014536">
        <property type="entry name" value="Snx9_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019497">
        <property type="entry name" value="Sorting_nexin_WASP-bd-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45827">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45827:SF5">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10456">
        <property type="entry name" value="BAR_3_WASP_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027744">
        <property type="entry name" value="Snx9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="11 12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="17">
        <location>
            <begin position="213"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="65"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR027744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR027744-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="549" mass="62188" checksum="3D6E2141902AD6F2" modified="2018-12-05" version="1">MALKAKVLYDFHAENPGEISIAENELVTLFSEEELEGWLEGENSKGEVGLFPASYVDIIRDQITSSASNNGYSSPKTITQSPSHTSYTSPDSHSQRKFKVGSGGGSSFNTSQGSDDDWDDDWDDSSSAQAGTTPPLYPVTTSLPGRGTGQQQQQQAKSSATVGRNLNRFSTFVKSGGEAFLLGEASAFVKDGDRICVVMGKYGPEWQEDPYPFTCTIDDPTKQTKFKGMKSYMSYGLTPTHTNVQVNRRYKHFDWLYARLVERFPVISVPHLPEKQATGRFEEDFISKRRKGLIWWMNHMTSHPVLAHCDVFQHFLTCGADEKAWKMGKRKAERDDLVGANFFLTISTPVVPLDLQEVESKIEGFKSFTKKMDENIVVVNTSINEFARKQITGFKKEYQKVGQSFKHLAQAFELDQQVYSTGLNKAICYTGEAYEAIGEYFAEQPRQDLEPISDLLDLYRGHLSNFPDIIHVQKGALTKVKDCPKKEGELHDRCNIISCATLAEIQHFHRTRVRDFRMQMQHHLRQQISFFQKITAKLEDALHKYDIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L4E5</accession>
    <name>A0A3B5L4E5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial proton/calcium exchanger protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Electroneutral mitochondrial K(+)/H(+)exchanger</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Leucine zipper-EF-hand-containing transmembrane protein 1</fullName>
        </alternativeName>
    </protein>
    <organism evidence="28 29">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Ca(2+)(in) + 2 H(+)(out) = Ca(2+)(out) + 2 H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72199"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LETM1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L4E5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005687"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005753.1">
        <property type="protein sequence ID" value="ENSXCOP00000005687.1"/>
        <property type="gene ID" value="ENSXCOG00000004426.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183167"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099093">
        <property type="term" value="P:calcium export from the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051560">
        <property type="term" value="P:mitochondrial calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000290">
        <property type="entry name" value="LETM1 and EF-hand domain-containing protein 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033122">
        <property type="entry name" value="LETM1-like_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044202">
        <property type="entry name" value="LETM1/MDM38-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059005">
        <property type="entry name" value="LETM1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009">
        <property type="entry name" value="LEUCINE ZIPPER-EF-HAND CONTAINING TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009:SF8">
        <property type="entry name" value="MITOCHONDRIAL PROTON_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26561">
        <property type="entry name" value="LETM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07766">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51758">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="15 24" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="18 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17 23" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="8 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Letm1 RBD" evidence="27">
        <location>
            <begin position="217"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="26">
        <location>
            <begin position="634"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="24">
        <location>
            <begin position="521"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00034214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00035046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS51758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="711" mass="81636" checksum="7C6FB327494961EB" modified="2018-12-05" version="1">MFVSFCTDRLDVLQLGSLARVFSVSPSPPLSEEYMATCQNLDPQRPYCAFAVASSSPAWRTTGRYFPGVRWIHTSRRRWDDSKLEKSLKSLKDRKMKLEEGGPVYSPTLDAEPVRRTIRQWVIDEIKHYYHGFRLLWIDTTIAGRMFWKILNGNPLSRRERRQFLRTCADVFRLLPFLVFIIVPFMEFLLPVALKLFPNMLPSTFETQSKKEERLKKELRVKLEMAKFLQDTIEEIALRNKAAQGNVTEEFSTFFQKIRDSGERPSNEQIIKFSKLFEDELTLDNLTRPQLVALCRLLELQSIGTNNFLRFQLIMKLRAIHADDKLIAEEGIESMSVTEVQSACRVRGMRSLGVTEERLREQLSQWLDLHLNQQIPTSLLLLSRAMFLPDTLSPADQLKTTLQTLPEMVTKEAQVKVAEIELSKVDNKTKLETMLQEEAAIRQDTKDREMERLADAAEKAAREDELELEVDGAKHSDAESALLQATRAAQSETLRDTAPVIEGSKDEEITKEEIHLLSDACSKLKEQKKLLTLEKEELEELKDDVQEYNEDLEEIKKEFSKTGQEKALKESKASKRLSKRVTRMIGRIDKIIGELEKDKVMLDGQAESGTTLPVGENLIGIDELINVMRQIQNIPEDKLQRIAKALDDNKDGKIDIDDAIKMVELIDKEDIDISTSQLADIMVMLQKEEKLMEKEKAKDKAEKEQAAKLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5P9</accession>
    <name>A0A3B5L5P9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="3 13">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="13">Homodimerization via the leucine zipper domains is required for autophosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="3 13"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5P9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005161"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005222.1">
        <property type="protein sequence ID" value="ENSXCOP00000005161.1"/>
        <property type="gene ID" value="ENSXCOG00000004039.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158243"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004706">
        <property type="term" value="F:JUN kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000076">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000085">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016231">
        <property type="entry name" value="MLK1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF35">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000556">
        <property type="entry name" value="MAPKKK9_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 13 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 16" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SH3" evidence="20">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="80"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="434"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="593"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="661"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="720"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="798"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="366"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="478"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="499"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="748"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="86"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
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            <dbReference type="ARBA" id="ARBA00022443"/>
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            <dbReference type="ARBA" id="ARBA00022527"/>
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        <source>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00047559"/>
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    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR000556"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50002"/>
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            <dbReference type="PROSITE" id="PS50011"/>
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        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005161.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="854" mass="95494" checksum="B5D4E8C4FA0B0DA5" modified="2018-12-05" version="1">SQRCSPQTRWWWGPRPLLLRRGDLVEVLSKDSLVSGDEGWWTGMIADRVGIFPSNYVSKRNGTEEEAGLREILEIDFSEFTLEEIIGVGGFGKVYRAVWRGAEVAVKAARRDPDEDVAQTLESVRQEAKLFAMLSHPNIMALLGVCLQEPNLCLIMEYARGGPLNRALAGKRIPPCTLVNWAVQIARGMHYLHDQAIVPIIHRDLKSSNILILERVEMEDLSNKTLKITDFGLAREWHHTTKMSAAGTYAWMAPEVIRSSTFSKGSDVWSYGVLLWELLTGEVPFRSIDGLAVAYGVAMNKLALPIPSTCPEPFARLMENCWSPDPHSRPLFTTILDQLTAIEESGFFEMPAESFHSLQDDWKLEIQEMFDQLRTKEKELRSWEEELTQAALQQKCQEEALRKREQELAEREIHIVERELNIIIHQLYQEKPRVESRQGKFRRNRLKLKDGNRISLPSDFQHKITVQASPSHDHRRSLLSSGSSPPTSPLMLPRLRAIQRKDEASGTDHTGGDRDGHTSSSIEHDGDEYCLSGQVPGTPPCRKSPGGGRRSELVLLGCGALLAAVGLGCNLLTLAPPEEGIKSRWDAFFHRTAGQRRSTSSPTRRLFHRESPLKTAAPALPERGLPSSYTLLSLSSVSDSNSTHSLLRSDSDELLICRPASAASRPPAPPPLSQRRAPVRTPVNSLVNIHVESFKRNPRQSLTPTHFPCVPPSARCLRRTPSDGAIKKNGPSTAGGFRGLRVDSPEVPRLPDPNVVYPPTPRRRCAPERPKTLDFVARPRPSPRPRCNMFRANGQTDSAVLPTPMEAPTPYSPRRNANLLDESDEGQFRDGTVPLCRPEFSLPKDSPYHPGFWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L633</accession>
    <name>A0A3B5L633_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">IF rod domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L633"/>
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        <property type="protein sequence ID" value="ENSXCOP00000005865.1"/>
        <property type="gene ID" value="ENSXCOG00000004553.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:CCR5 chemokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:intermediate filament binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of intermediate filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IF_rod_dom"/>
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        <property type="entry name" value="Nestin"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Filament"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
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    </dbReference>
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        <property type="entry name" value="IF_ROD_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="4">
        <location>
            <begin position="18"/>
            <end position="277"/>
        </location>
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            <end position="172"/>
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            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="277" mass="32900" checksum="E7785CF364DCB4AA" modified="2018-12-05" version="1">MELHGVHKIFLPNHLGEEKQQMLNLNRRLETYLSWVKCLEEENEMLANEIKVFRHNNQGTLTSRRGLEEELRQTRLEMDATWRERVLTEVEVQKLTEEIQALEMQRQMEAEAKMLAKTKMEQSRKELEDEQRAQIWLREKVNQLEHEMKFLIQTHEEDVTHLEATLSQSRAEMLPNQIHRSKQTPDLLKMGHEFSQRATRAWQEASESYQGQLAQLEESLDQARSRLSQVCLEKRESQLKLQTLEKEIVSAQDVRLHLEKTVAQRSEEHCQEIQQIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7L8</accession>
    <name>A0A3B5L7L8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L7L8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008298"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008397.1">
        <property type="protein sequence ID" value="ENSXCOP00000008298.1"/>
        <property type="gene ID" value="ENSXCOG00000006352.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157623"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="Region D6 of dynein motor"/>
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        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DYNEIN HEAVY CHAIN 6, AXONEMAL-LIKE-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Dynein_C"/>
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        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-0243">Dynein</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynein heavy chain region D6 P-loop" evidence="13">
        <location>
            <begin position="160"/>
            <end position="269"/>
        </location>
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    <feature type="domain" description="Dynein heavy chain AAA lid" evidence="14">
        <location>
            <begin position="297"/>
            <end position="387"/>
        </location>
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    <feature type="domain" description="Dynein heavy chain C-terminal" evidence="15">
        <location>
            <begin position="481"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03028"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF18198"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF18199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="663" mass="74927" checksum="4A0099CE3669F178" modified="2018-12-05" version="1">LNDMSLIDPMYQFSLDAYINLFMLSIAKSKQAYSLRVEERINNLNDYHTYSVYRYTCRGLFEAHKLLLSFQMCVKILEVAGKLNMDEYNIFLRGIVVRNTQTSCDLDHNYNELKKMVIVRFLRQDRVSFCITSFIIKNLGPRFVEPPVLDMKAVVEESTSCSPLIFVLSPGVDPTGSLLQLAKASGMSHQFSALSLGQGQAPIKLCCDFPGHWVFLANCHLSLSWMPELDKLIKELPVQNPEPNFRLWLSSSPHPDFPITILQAGIKMTTEPPKVHKHHCKHENLPQFNRCTKGRLYKKLLYALCFFHSILLERKKFRQMGWNIIYGFNDSDFEVSENLLCLYLDKYEEIPWDALKYLIAGVNYGGHVTDEWDRRLLTTYINDYFCEGGPLSSFQEHINTLPATEHPEVFGEHPNADIASQIAETRTLFETLLSLQPQHTGTAATGAGARPKDAGNSGFIKNTRTLTSYCVDSTSVSVLQAYPSLKPLAAWTRDLSQRVDQFARWAEKVQPPVLFWLSGFTFPNGFLTAVLQFTARQHNVSAHRRLCVPFLSVSSLIQDGVYVKGLYLEGAGWDRKNSCLVEADHLLKCVDLCNDAIVISTPSPRAVSFPSVGMYLCPCYYFPVRAGKDGRPAFVVAIELKSGAVSPDHWVKRGTALLLSLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L804</accession>
    <name>A0A3B5L804_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Adhesion G protein-coupled receptor G4b</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L804"/>
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        <property type="protein sequence ID" value="ENSXCOP00000008433.1"/>
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    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Adhesion G protein-coupled receptor G4b"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="GAIN_B"/>
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        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="GPCR_2-like_7TM"/>
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        <property type="entry name" value="GPCR_2_secretin-like"/>
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        <property type="entry name" value="GPS"/>
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        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF277">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR G4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="7tm_2"/>
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        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
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            <end position="199"/>
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            <end position="222"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="285"/>
        </location>
    </feature>
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            <begin position="1"/>
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    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="8">
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            <begin position="146"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50221"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="343" mass="38756" checksum="305357BBEA1ED2CF" modified="2018-12-05" version="1">YPSDGMVAFISLPSALQNNFPQNGLNQNQPRIQFQFYANPLLFKEPFLSTFVVSASVSNASSPIKDLHEEVKVMLHHLQPSVHDRQVQCVYWNFNKNGHGGWDDHGCRKYNSTPDYTTCLCDHLTHFGVLLDVSRTQLDPLHEQILTIITYVGCGVSSLFLGITILTYIAFKLRRDYPSQILINLSLALLGLNLMFLINSWLSSWSVYSVCVAAASLLHYFLLASFTWMGLEAVNMYFALVKVFNVYVPSYILKFCALGWIPLVICIMVLIVNRDAYFVVVLIQIRRMRFNSPAGTRRGVLQDLKGAASLTFLLGLTWTVGFFTWAPARAVLMYLFAGLNTLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8D2</accession>
    <name>A0A3B5L8D2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Furry homolog a (Drosophila)</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000007077"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007165.1">
        <property type="protein sequence ID" value="ENSXCOP00000007077.1"/>
        <property type="gene ID" value="ENSXCOG00000005466.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00610000086058"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025614">
        <property type="entry name" value="Cell_morpho_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025481">
        <property type="entry name" value="Cell_Morphogen_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045842">
        <property type="entry name" value="Fry_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039867">
        <property type="entry name" value="Furry/Tao3/Mor2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029473">
        <property type="entry name" value="MOR2-PAG1_mid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295">
        <property type="entry name" value="FURRY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295:SF29">
        <property type="entry name" value="PROTEIN FURRY HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19421">
        <property type="entry name" value="Fry_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14225">
        <property type="entry name" value="MOR2-PAG1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14228">
        <property type="entry name" value="MOR2-PAG1_mid"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14222">
        <property type="entry name" value="MOR2-PAG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cell morphogenesis protein N-terminal" evidence="2">
        <location>
            <begin position="132"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1175"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1598"/>
            <end position="1658"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1752"/>
            <end position="1860"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis protein C-terminal" evidence="3">
        <location>
            <begin position="2000"/>
            <end position="2251"/>
        </location>
    </feature>
    <feature type="domain" description="Protein furry C-terminal" evidence="5">
        <location>
            <begin position="2276"/>
            <end position="2932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1525"/>
            <end position="1550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1712"/>
            <end position="1732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1921"/>
            <end position="1958"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2302"/>
            <end position="2336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2450"/>
            <end position="2484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1525"/>
            <end position="1535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1713"/>
            <end position="1731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1944"/>
            <end position="1953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="2302"/>
            <end position="2313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2314"/>
            <end position="2336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2451"/>
            <end position="2470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="2932" mass="331547" checksum="EE04EC7D43E340D9" modified="2018-12-05" version="1">PPIPLVCCPQGEKGPPAMLPITIDPESRPGEYVLKSLFANFTTVSERKIRIIMAEPLEKPLTKSLQRGEDPQFDQLISAMSSLAEYSLPSILRTLFDWYKRQNGLEEELHEYRPRVNTKSKNDEQQRDYLLERRDLAIDFIFSLVLIEVLKQMPLYPALDGLVNEVINLAFKHFKYKEGYHGPNTGNMHTVADLYAEVIGVLAQSKFPAVKKKFMTELKELRQKEQNPYVVQSTISLIMGVKFFRVKMYPVEDFEASFQFMQECAQYFLEVKDKDIKHTLAGVFVEILVPVAAAVKNEVNVPCLRNFVDSLYDTTLDLSSRKKHSLAFYPLVTCLLCVSQKQFFLNRWHVFLNNCLSNLKSRDPKMARVALESLYRLLWVYMIRIKCESNTATQGRLSTVITTLFPKGSRSVVPRDMPLNIFVKIIQFIAQERLDFAMKEIIFDLLCVGKPAKAFSLNPERMNIGLRAFLVIADKLQQKDGEPPMPNTNCSLPSGNTLRVKKTYLSKTLTDEEAKVIGMSQYYVHVRKAIDSILRHLDKEVGRCMMMTNAQMLNKEPEDMITGERKPKIDLFRTCVAAIPRILPDGMSKPELIDLLSRLTIHMDDELRLIAQNSLQSLLVDFSEWRDDVLFGFTNFLLREVQDTHQGLLDTSLRLLLQLLTQWRITLASSGKSHDLAKLHALELLNSCSGHKMPADRAPHSTVLHAVEGLALVLLCSCQLSTRRLAIAVLKEIRSLYLSIGQTEDDEKPMIEIMDQLSPVIMESFINVAVSDAAALPAGHHVDLQWLVEWNAALVNSQYDIMSPSHVWLFAQSVKDPWVLCVFCLLRQDNLPKFCPTALSYAWPYAFTRLQMLMPLVDPNSPVNAKKNGTSGSGESYVTLWRNYLILCFGVAKPSIMSPGHLRASTPEITTSTPDSTISHDNKVIGSPSVAWLLKQLVPLMRTESIELTESLVLGFGRTNLLVFRELVEELHPLMKEALERRPENKKRRERRDLLRLQLLRIFELLADAGVISDSTNGALERDTLALGALFLEYVDLTRMLLEAENDKEAEILKDIRAHFSAMVANFIQCVPVHQRRFLFPQQSLRHHLFILFSQWAGPFSIMFTPLDRYSDRNHQITRYQYCALKAMSAVLCCGPVFDNVGLSQDGYLYKWLDNILACQDQRVHQLGCEVVILLLELNADQVNLFNWAVDRCYTGSHQLASGCFKAIAAVFSSSKNYPCDIVTLLNLVLFKTSSTNRELYEISMQLMQILEDKLFMYSKKTAELKPSNILYGTHGPLPPLYSVSLPQLSTQLARMYPELTLPLFSEVSQRFPTTHSNGRQIMLSYLLPWLGNIELVDSGLLLPVFAPSNPSCESSCQLTTTDSLHSLKGTGWGSLQATSMVLNNLMFMTAKYGDDLPGPEMENVWNALVSNEKWSNNLRTTLQFIISLCGVSSETTLLPYIKKVVIYLCRNNTMQTMEELLFELQQTDPVNPVVQHCDSPPFYRFTATSKASVVASGTTSSSNTVIACQDNFPDTDDIKTMKENEERAHSRLESRYSNSSGGSYDEEKNEPLPPYVDWLTFVIETNKPHPLPMPLNGGCWAPLVDFLPETITPRGPLHRCNIAVIFMTELVVDHGVREDWTLHLPLILHALFLGMDHYRPEVFEHSKRLLLHLFIVLFCNNNFQTVASVLLQTREVNVLKTLTCTSSLQMEYSPAGGCDFLRECQASPVPDSGLSSSSTSSSLSLGGSSSNLPEITQEMEEFVSSCKMNEKSNKLIEFLITRPYGPLWCHEDISPKNQVSKSTAQLTNFLRHVVSVFKESKSDYFLEQQLSEVALQTALCSSSRHYAGRSFQVFRALRQPISAHAVSDLLSRLVEVIGEHGEEVQGYVMEVLLTLESVVDNLAECLKNNDLMVFFTRFVLPLSSSPDFLTSFKQLSNRKSTGQLSLRREERSRHQRSSSVPKKFGEADRCSDPPRSATLDRYQACEQQVLLARMRSSSSPKDNTTDPMSINHPSNLLATVFWVAVSLMESDFEFEYQMSLRLLNKLLTNMSLDKQENREKLEKLQSQLKWSSFTGLQQLLLKGFTSTSTTDLTLQLFCQLTPVSRVPVVDTSQAIGFPLNVLCLLPHLVQNFDAPSPFCQDVAEKIAQVCLEEKNAKLSNLAHVMTLYKTHTYTRDCFSWVNVVCRYLHETFSDITLNLVTYMAELLEKGLPSMQQTLLQIIYSLLSHMDLSGIQAKPFNIEVLKTIEKFVQTAHWREALNILKLVVSRSASLVQPSLPQSDFSYEDTSRMWDRSSKALPGKTLDFHFDISETPVIGRRYDDLKRSPGHDVRSMATAVTRSTSSTSSGSSSNNVLVPVSWKRPQSSQKRTREKLVHVLSLCGQEVGLTKNPSVIFSSCGELDLIEHQPSLVSSDEGTRELENMDDTTSEQQFRVFKDFDFLDVELEDGEGETVDNFNWGVRRHSMDSLDQSDLQPLEESQLSSSMPSLSKITHEDSDESSEDDSLAASQILSHSQIVSLCDNVTLISNLNEAKTFKPFLLMILFLCPSFVFQYEVSQEDEDGNVHEDDLSLSINELPSEYQFCLIFVFSLPEEEKDDMLESRSSPPPSPFFSAILAAFQPTVCDDAEEAWRSHLNQLVSDSDGSCAVYTFQVFSSLFQSIQAKFCSLTCDAVSYLGDGLRGLGSKFLRSSEILASCSECPTLFIDADTIMSYGFLEKMKFSVLELQEYLDTYNNRKEATISSSFNIYCKFVFDPKPFSFQQLELCQRLYKLHFQLLLLFQSYCKLIEQVHAISSIPELTNMSRELNELKSNLKTAAASVSNEKISTFDTSCSETILSSSEAAVQAVLEGLKNNEFSKAILYIRECRTTWPNDIFGSHSEDEIQTLLNIYFRHQTLGQTGTFALVGLKQDLTEISLKLMELNCDTRDMIRRAQGYKTITAFLPDSRVSGSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L952</accession>
    <name>A0A3B5L952_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 20">O-phosphoseryl-tRNA(Sec) selenium transferase</fullName>
            <ecNumber evidence="6 20">2.9.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 20">Selenocysteine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17 20">Selenocysteinyl-tRNA(Sec) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18 20">Sep-tRNA:Sec-tRNA synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">SEPSECS</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 20">Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 20">
            <text>O-phospho-L-seryl-tRNA(Sec) + selenophosphate + H2O = L-selenocysteinyl-tRNA(Sec) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:25041"/>
            <dbReference type="Rhea" id="RHEA-COMP:9743"/>
            <dbReference type="Rhea" id="RHEA-COMP:9947"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16144"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:78551"/>
            <dbReference type="ChEBI" id="CHEBI:78573"/>
            <dbReference type="EC" id="2.9.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20 22">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 20">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route): step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homotetramer formed by a catalytic dimer and a non-catalytic dimer serving as a binding platform that orients tRNASec for catalysis. Each tetramer binds the CCA ends of two tRNAs which point to the active sites of the catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 20">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 20">Belongs to the SepSecS family.</text>
    </comment>
    <dbReference type="EC" id="2.9.1.2" evidence="6 20"/>
    <dbReference type="RefSeq" id="XP_027894408.1">
        <property type="nucleotide sequence ID" value="XM_028038607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L952"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006286"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006361.1">
        <property type="protein sequence ID" value="ENSXCOP00000006286.1"/>
        <property type="gene ID" value="ENSXCOG00000004860.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114157547"/>
    <dbReference type="GeneTree" id="ENSGT00390000007332"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00898"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098621">
        <property type="term" value="F:O-phosphoseryl-tRNA(Sec) selenium transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001717">
        <property type="term" value="P:conversion of seryl-tRNAsec to selenocys-tRNAsec"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001514">
        <property type="term" value="P:selenocysteine incorporation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000070">
        <property type="entry name" value="O-phosphoseryl-tRNA(Sec) selenium transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019872">
        <property type="entry name" value="Sec-tRNA_Se_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008829">
        <property type="entry name" value="SepSecS/SepCysS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03531">
        <property type="entry name" value="selenium_SpcS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12944:SF2">
        <property type="entry name" value="O-PHOSPHOSERYL-TRNA(SEC) SELENIUM TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12944">
        <property type="entry name" value="SOLUBLE LIVER ANTIGEN/LIVER PANCREAS ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05889">
        <property type="entry name" value="SepSecS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017689">
        <property type="entry name" value="SepSecS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 20" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 20" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12 20 22" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 20 22" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="14 20" id="KW-0711">Selenium</keyword>
    <keyword evidence="10 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 20 22" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="466"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>tRNA</name>
            <dbReference type="ChEBI" id="CHEBI:17843"/>
        </ligand>
        <ligandPart>
            <name>tRNA variable arm</name>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>tRNA</name>
            <dbReference type="ChEBI" id="CHEBI:17843"/>
        </ligand>
        <ligandPart>
            <name>tRNA discriminator base</name>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>tRNA</name>
            <dbReference type="ChEBI" id="CHEBI:17843"/>
        </ligand>
        <ligandPart>
            <name>tRNA acceptor arm</name>
        </ligandPart>
    </feature>
    <feature type="site" description="May act as a substrate filter by repelling compounds with a negatively charged alpha-carboxylate" evidence="22">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="22">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00021963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00026053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00032693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRNR" id="PIRNR017689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR017689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR017689-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="485" mass="53024" checksum="662D757DA4C3993B" modified="2018-12-05" version="1">MNSENVTLCEKTVSSSYIRQGSQARRGHEQLIRHLLDQGKCPEEGWSESTVELFLNELAVMDSNNFLGNCGVGEREGRVASSLVARRHYRLIHGIGRSGDIAAVQPKAAGSSLLNKLTNSVVLDILKISGVRSVASCFVVPMATGMSLTLCFLTLRHRRPKARYIIWPRIDQKSCFKSMITAGFEPVVVENVVEGDELRTDLEAVQRKIDDLGAENILCVHSTTSCFAPRVPDRLEELATMCAKHDIPHIVNNAYGVQSSKCMHLIQQGARVGRIDAFVQSLDKNFMVPVGGAIIAGFDESFVQEISKMYPGRASASPSLDVLITLLTLGSNGYKRYLSERKEIYSFLAEELKSLASAHGERLLHTPHNPISLAMSLDGLQASSNQAVTQLGSMLFTRQVSGARVIPLGKEQTISGHTFRGFMSHSESYPCPYLNAASAVGITRGDVTLCMKRLDKCLKALKKHGKTAGSCSTEPQADQEAPDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9J1</accession>
    <name>A0A3B5L9J1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutamine-dependent asparagine synthetase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9J1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000004499"/>
    <dbReference type="Ensembl" id="ENSXCOT00000004555.1">
        <property type="protein sequence ID" value="ENSXCOP00000004499.1"/>
        <property type="gene ID" value="ENSXCOG00000003557.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001994"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000039">
        <property type="entry name" value="Asparagine synthetase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050795">
        <property type="entry name" value="Asn_Synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772">
        <property type="entry name" value="ASPARAGINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772:SF23">
        <property type="entry name" value="ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="5">
        <location>
            <begin position="2"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000004499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="333" mass="38043" checksum="A070D4697078AE2E" modified="2018-12-05" version="1">MCGIWALFGSDECLSVQCTCAMKIAHRGPDAFRFENVNGFTNCCFGFHRLAIVDQLYGMQPLRVKKFPYLWLCYNGEIYNHQLKTRFDFDYQTKVDGEVLLHLYDRFGVQKMASLLDGVFAFILLDTANRKVHLGRDTYGVRPLFKFLTDDGFLAVCSEAKGLVEITHSMETAASIVPFLPGHFEVFDLKLIGKVQSVQFEQFHCCTREPAHALYDTVGGLSSDFGEETVKRNIRSLFENAVRKRLMAHRRIGCLLSGSCCRLNQKMAYTDSKNLFDKRTTFSEQYSRQNVQKHIHFVFEIRKTVCKYVLPKTHQVAVLASPGSNSVKKIISH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LD59</accession>
    <name>A0A3B5LD59_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RAB11 binding and LisH domain, coiled-coil and HEAT repeat containing</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LD59"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008470"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008576.1">
        <property type="protein sequence ID" value="ENSXCOP00000008470.1"/>
        <property type="gene ID" value="ENSXCOG00000006488.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004385"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032367">
        <property type="term" value="P:intracellular cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000080">
        <property type="entry name" value="lisH domain and HEAT repeat-containing protein KIAA1468 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000175">
        <property type="entry name" value="lisH domain and HEAT repeat-containing protein KIAA1468 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040362">
        <property type="entry name" value="RELCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32059">
        <property type="entry name" value="RAB11-BINDING PROTEIN RELCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32059:SF0">
        <property type="entry name" value="RAB11-BINDING PROTEIN RELCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="HEAT" evidence="1">
        <location>
            <begin position="902"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="125"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="310"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="167"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1114" mass="123672" checksum="F5BEEBD421201799" modified="2018-12-05" version="1">MASVNPFDVSDSDEEAERRPNETVDTERSPSEGAPGPPPGNAFSLPADAEPPTLLLSSNRTSPSGEGMSVSAAATSTMAGGVETRVSVDVIAAQLLRDQYILTALEFHTELLEAGRELPRLRDYFSNPGNFERQSGTPPAKDQVLGPGGPLNRAGSISTLDSLDFARYSDDGNRESDERVAESEVASQERKNFKSSPEIQEPIRPLEKRALNFLVNEYLLKNEYKLSSITFSDENDDQDFELWDDVGLNIPKPPDLLQLYRNGGSTLSSPTDKVDASVGVAFGDLPGNCVSREPQKKPDLSQQVCISKVVQELEYQLNLLNSEKQSLAEQIKKLLSLLQLGFVCTSVILWSEQLPSPFFGELTGIVERCTIMMVSNLTFSCYSRKLSPAFQQALLSFCRMCSESRLGAEVSRIADSEESVMLMLGRCLPHIVPNVLLAKREELIPLILCTACLHPEPKERDQLLHILFNLIKRPDDEQRQMILTGCVAFARHVGPTRVEAELLPQCWEQINHKYPERRLLVAEACGALAPYLPKEIRSSLVLSMLQQMLTEDKADMVREAVVKSLGIIMGYIDDSDKYAQGFELMLLSLGDPSERVVNAVHQVFIPAFAAWTTELGTLHTALIPSLLARIEKLLTQGEHGLDEHKLHVFLSALQSLIPPLFAVVLQNAPFTSRAKPHEDIPAIEVTRFPRPASPLQDMAIIIGNRELLSSLLLLYDHQLEHEGTTGWESLLWVVNQLLPQLIEIVGRINVTSSTCVHEFSRFFWRFCRTFGKIFTNTKVNSIQLPRFSLLSYASAGNGILTKATVPIYATGVLTCYNQEEDRKLLVGFLEDVMTTLSLSHAPLDSLKASFVELGANPVYHELLLTVLWYGVVHTSALVRCTAARMFELLVKGVNETLVAQRVVPALITLSSDPEISVRIATIPAFGTIMETVTQKELLERVKMQLASFLEDPQYQDQHSLHMEIIRTFGRVGPNAEPRFRDDFVLPHLHKLALANNSQSVESKRIDIAIHLFEAYSALSCCFISEEVMVNHFLPGLRCLHADMEQLSPEHEVILGSMIKECEIKVENRGISDAQGSISIASSLVGEDAKTKFLSKMGQLTTSGAMLANVFQRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDH9</accession>
    <name>A0A3B5LDH9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDH9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008998.1">
        <property type="protein sequence ID" value="ENSXCOP00000008892.1"/>
        <property type="gene ID" value="ENSXCOG00000006783.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156081"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17387">
        <property type="entry name" value="MFS_MFSD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF32">
        <property type="entry name" value="HIPPOCAMPUS ABUNDANT TRANSCRIPT-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="261"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="291"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="358"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="406"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="15"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="428"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="451" mass="49032" checksum="167C93C5FCE463D2" modified="2018-12-05" version="1">VKVRRLQSQGIGRPSVYHAVVVIFLEFFAWGLLTTPMLTVLHETFPQHTFLMNGLIQGVKGLLSFMSAPLIGALSDVWGRRSFLLITVFFTCAPIPLMRLSPWWYFAMISMSGAFSVTFSVIFAYVADVTDERERSTAYGLVSATFAASLVTSPAIGAYLSAWYGDNLVVLLATLISLADICFILLAVPESLPDKMRLNTWGTPFSWEQADPFASLRKVGQDSTVLLICITVFLSYLPEAGQYSSFFLYLRQVINFSSTTIAVFIGVVGILSIVAQTLFLTLLMRYLGNKNTVLLGLGFQILQLAWYGFGSEPWMMWAAGAVAAMSSITFPAVSALVSQSADPDKQGVVQGMITGIRGLCNGLGPALYGFIFFLFNVELNTLDPIQEDPCLVCPVLQQALIPGPPFLLGACTVVVAFLVALFIPEKTCCSSSSSSSSDAEDSEPLLHDSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKY9</accession>
    <name>A0A3B5LKY9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="9">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the Deltex family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="9"/>
    <dbReference type="RefSeq" id="XP_027873290.1">
        <property type="nucleotide sequence ID" value="XM_028017489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKY9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008539"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008644.1">
        <property type="protein sequence ID" value="ENSXCOP00000008539.1"/>
        <property type="gene ID" value="ENSXCOG00000006534.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114144546"/>
    <dbReference type="KEGG" id="xco:114144546"/>
    <dbReference type="GeneTree" id="ENSGT00940000161404"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09633">
        <property type="entry name" value="Deltex_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.130:FF:000001">
        <property type="entry name" value="Probable E3 ubiquitin-protein ligase DTX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039396">
        <property type="entry name" value="Deltex_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039399">
        <property type="entry name" value="Deltex_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039398">
        <property type="entry name" value="Deltex_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12622">
        <property type="entry name" value="DELTEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18102">
        <property type="entry name" value="DTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="17"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="198" mass="21928" checksum="E6CC978109D02688" modified="2018-12-05" version="1">MENPANNTKHADSTGDCAICLERIQRKKTLKCQHSFCSECIDSVFRLKPACPICNTFHGVYTGTQPQGTMTVTHSFLKLPGFENCGSIVIQYSFPGGIQGAEHPNPGVRYSSTSRIAYLPDCAEGQKVLRLLRKAFDRRLTFTVGRSATTGLNNVITWNDIHHKTSINGGPESFGYPDPAYLFRVQEELRLKGVTEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLD3</accession>
    <name>A0A3B5LLD3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Sushi domain containing 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLD3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011737"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011871.1">
        <property type="protein sequence ID" value="ENSXCOP00000011737.1"/>
        <property type="gene ID" value="ENSXCOG00000008865.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013892"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057598">
        <property type="entry name" value="Fn3_PTPRU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051622">
        <property type="entry name" value="R-tyr_protein_phosphatases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24051">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24051:SF5">
        <property type="entry name" value="SUSHI DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23144">
        <property type="entry name" value="Fn3_PTPRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2 10" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0768">Sushi</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="12">
        <location>
            <begin position="91"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="13">
        <location>
            <begin position="143"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="8"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="42637" checksum="A902E9E86FDCEB51" modified="2018-12-05" version="1">VLDVCATCHPNATCEDKPDGSGKVCNCNYGFVGNGRTHCQKDECQIGASKICGQQTTCHNTYGSYYCTCLSGYSPSNNMAVFIPNDGTHCQIDECRITGLCGEGGQCRNLEGSFDCSCQVGYRVHNGAEPFRPQGGGASCKVVDCGPPVPVEDSVLLSITVTTYGGVARFGCEEGFVWRRGDNSSVCGADGSWSEPSLVCEKCGPVPVLAHSEVVWHNRSVAIHRCAAGYHSWRGVNASVCGGSGLWLRATLNLVICLRLLPLTNYSISITAAAARFTATVTANTPVVHYTELETPVPTLRLKRSPNTLDPIFYQIFVLPVEELVVFDCSSPASLDPSSRVESPAEYMTAQLGVQSLGMEVNFTVGDGVLYGGFYNAPLQSGNTYFVVLRVVSRWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLI2</accession>
    <name>A0A3B5LLI2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BRISC and BRCA1 A complex member 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLI2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011797"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011933.1">
        <property type="protein sequence ID" value="ENSXCOP00000011797.1"/>
        <property type="gene ID" value="ENSXCOG00000008911.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016934"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070531">
        <property type="term" value="C:BRCA1-A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070552">
        <property type="term" value="C:BRISC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045739">
        <property type="term" value="P:positive regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21502">
        <property type="entry name" value="vWA_BABAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026126">
        <property type="entry name" value="BABAM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15660">
        <property type="entry name" value="BRISC AND BRCA1-A COMPLEX MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15660:SF1">
        <property type="entry name" value="BRISC AND BRCA1-A COMPLEX MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="313" mass="34791" checksum="A5742ED1A48AA869" modified="2018-12-05" version="1">METPEPGPADGEERPVELRPRTRSNPEGAEDRRSSTGSLGGNGNPTMPQPAVGNRVEGEGEASTTDSPLTAGTTPVLSMSIPPPAEYQLRVPRVNCPEKVIICLDLSEEMSLPKLESFNGSKTNALNISQKMIEMFVRTKHKIDKRHEFALVVINDDALWISLTSCKLCLCFSNFLKFKIELPSMENVQTIPPPYVVRTVLIYSRHAGPLQFNPSEALSKMLQSPYFFFDVVYLHNGMEEQGDETSWRTYSSFCTLDSKGMCYRFEVSLSGPAIELHNCMAKLLAHPLQRPFQSHASYSLLEGDDTPDTEATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLW5</accession>
    <name>A0A3B5LLW5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LLW5"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011843.1">
        <property type="protein sequence ID" value="ENSXCOP00000011710.1"/>
        <property type="gene ID" value="ENSXCOG00000008842.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182847"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005549">
        <property type="term" value="F:odorant binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000024">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052921">
        <property type="entry name" value="GPCR1_Superfamily_Member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26451:SF869">
        <property type="entry name" value="OLFACTORY RECEPTOR 530-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="208"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="53"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="329" mass="37490" checksum="C49A876EA61B4B27" modified="2018-12-05" version="1">MSYLSNMNTLPASIFNSTFVRPAEFYLGGFYNIPHVKYYYVFLCFVYIMTVLGNGFLLSVIYKVKTLHSPKYIIVFNLAFTDLCGSTALIPKLLDTFLFNRRYIVYEACLAYMFFVLLFTSLQSWTLVTMAYDRLIAICFPLRYHSIITTKSVVVILLCCWMFMVGITATSAGFINRLSFCSSIEIKSFFCDHGPIYQLACNDKSYSYILATIALIVVMCIPLILIFSSYVCIAIALSRTISREERLRALETCTSHLILVAIFFLPFVGTNVAVLTSYIHPNARIINSTLTFTVPSLLNPIIYSLKTEEVRNAIKKLCRRAKMRNINAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LML6</accession>
    <name>A0A3B5LML6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rap-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="component" value="Unplaced"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Signal-induced proliferation-associated 1-like protein 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Rap/Ran-GAP"/>
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    </dbReference>
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        <property type="entry name" value="Rap1_Ran_GAP"/>
    </dbReference>
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        <property type="entry name" value="RAP GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711:SF64">
        <property type="entry name" value="SIGNAL-INDUCED PROLIFERATION-ASSOCIATED PROTEIN 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
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    <feature type="domain" description="Rap-GAP" evidence="6">
        <location>
            <begin position="279"/>
            <end position="496"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="18"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="825"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50085"/>
        </source>
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    <evidence type="ECO:0000313" key="7">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="880" mass="99164" checksum="C4D96D43A0791A23" modified="2018-12-05" version="1">MQSDDLFIRKFRRQNMRPPLATVNFDPKREAGVVEWPPRRSGGDSPKPAKPPKPEGLSKKSKLMPPQIPQPSTYDHLGGGTWVRNFAHYDVQSILFDLTESTPLLLSCPHFRNETGGEEQVGLGRSAGRRELWSTLRTPNDAVSVLEEPRESNIQLQGKSNYFIEHADLGAHYYRKYFNLKEHQNFFGIDDRLGPVAISFRRDEKEGSSGAQYNYRIIFRTTEMKTLRGSILEESVPSAARHTTPRGLSPKRLLEFIMPELNLHCLRLASNSPKVRDTLLKLDEQGLNFQRKVGVMYCRAGQSSEEDMYNNESSGPAFEEFLDLLGERVRLKGWEKYRAQLDNKTDSTGTHSLYTRYQDYEIMFHVSTMLPYTANNTQQLLRKRHIGNDIVTIVFQEPGALPFTPKSIRSHFQHVFIIVQVHEPGTDHAYYRVAVTRSKDIPLFGPLFPRGARFPRSPAFRDFLLAKVVNAENAAEKSEKFRSMATRTRQEYLKDLAENYVTTTPIDSSTKFPLLSLGSKRKDKMKGAKGAELHSAGALVWAVMVSCRDDSEAGEQQKFPCLLGVSAESVVLIERGSRRVVFNCSCRDVIGWKAVTETKDGGPLQKLCFSFTCPVVLQLVTRGCEALEVTPLRDGVGQPGFLMNEEGFVTELQRFCYAESGGLQLWARVVRLCGHSLVHLSPEERSRLLRMAHKIHITVIPPDENGKPRRLDRSLSTHQRQTVNLGGGHTAGVKAANELEGVSGTRWSRKRQESEIGHSSLFLSETTDSTDEEWQSIADLATACRSILEALSKEGEPSLYLSPPTSRSCHSNLSDSFCSLLCADVFQEKEDKAVLQAEVQSLRQNNQRLQEESQSTVARLIKVTECLTNTPKADYTKTFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LP53</accession>
    <name>A0A3B5LP53_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Neoteleostei</taxon>
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            <taxon>Ovalentaria</taxon>
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            <taxon>Cyprinodontiformes</taxon>
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            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LP53"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013863"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014033.1">
        <property type="protein sequence ID" value="ENSXCOP00000013863.1"/>
        <property type="gene ID" value="ENSXCOG00000010496.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070593">
        <property type="term" value="P:dendrite self-avoidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000023">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase delta isoform X2"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig_I-set"/>
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        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
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        <property type="entry name" value="BASIGIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10075:SF103">
        <property type="entry name" value="ROUNDABOUT HOMOLOG 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="9"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="2">
        <location>
            <begin position="111"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="192" mass="21143" checksum="60E3A7F885A2943D" modified="2018-12-05" version="1">MFVFHVGIPNFIKSPDDQTGIYGGVASFICQATGDPKPLITWKKEGKKVNSQRFEVKEFDDKSGSVLRIQPLKTNRDEAIYECTASNSVGAIAAPAKLIVLEDDQIPAGFPNIDMGPQLKVVVRTNTATMLCAASGNPDPEIFWFKDMLPVDISSSNGRIKQLRSGILFPPSSIVYLILLVGKKDLFLFLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPM9</accession>
    <name>A0A3B5LPM9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPM9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012705"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012859.1">
        <property type="protein sequence ID" value="ENSXCOP00000012705.1"/>
        <property type="gene ID" value="ENSXCOG00000009593.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159792"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048598">
        <property type="term" value="P:embryonic morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002573">
        <property type="term" value="P:myeloid leukocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000141">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000002">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF9">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="10">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="342"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="95"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="517"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="163"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="177"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="251"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="284"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="313"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="517"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="542" mass="60996" checksum="5F5E660D81983FCE" modified="2018-12-05" version="1">MFAKKKKSRIQISAPSNFEHRVHTDFDEQEQKFVGLPRQWQSLIEDTAKRPKPFIDVTVITTVEPRKTIVRGTKLGADGSLTWLLDEFDTMSVTRSNSLRRGSPPTQPRRDSSSAGGEQENGDPRHRHHPDRDRLRQDYPGGGDPQHGQRGVQPRDEGGQGRQGRPQQQPRGQEPSRAYRERSGSGQQPRRDPRDRDQDQVIRRDQASDHRPKSSYAARDGSPTSPRDKRPLSGPNIRTPNLPVTEGVMKTAQQNTRPFNTYPRTDSDGGRSPTGQVVRHHESSPQNGPTSSSARGSSKPPVSLPHSHHGRSPQREPQRVSHEQFRAALQLVVDPGDPRTYLDHYIKIGEGSTGIVCIATVKTTGKLVAVKKMDLRKQQRRELLFNEVVIMRDYHHDNVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIATVCLSVLKALSVLHTQGVIHRDIKSDSILLTHDGRVRSRRTAKTHNALQVRELQHWLKEREPAAVVLSLCSESNQPNPLSNLFPSSPVGALHQESLKKTTQKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPN5</accession>
    <name>A0A3B5LPN5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPN5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012952"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013112.1">
        <property type="protein sequence ID" value="ENSXCOP00000012952.1"/>
        <property type="gene ID" value="ENSXCOG00000009808.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163009"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000002">
        <property type="entry name" value="Transmembrane protease serine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017305632" description="Peptidase S1 domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="4">
        <location>
            <begin position="25"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="267" mass="29467" checksum="554A1D550B57FF03" modified="2018-12-05" version="1">MAVGKLLALLVLLQSLMGAGVRSSIIGGGDAPKGRWPGLVYLDIKTDSGERKWHCSGSILNQKWIMTAGRCWDETRWDRTGVWIGTYELGMPSERYMNVDLVDRLPEFRAAGNGFVNDIALLRLKYPLEFSKNVAPVDLPNDDDIFDSSSECWIAGWGDIGKGTKPLQEVQISILPRKDCKAKYPTMTDAMLCAGDPKGKKNPCDGDYGDPLMCRTARGFVQVGIMSYGSPNGCEVAGFPSIYTRVSSFMGYIKDRMKQLDEELASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPX9</accession>
    <name>A0A3B5LPX9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytosolic endo-beta-N-acetylglucosaminidase</fullName>
            <ecNumber evidence="3">3.2.1.96</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>an N(4)-(oligosaccharide-(1-&gt;3)-[oligosaccharide-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc)-L-asparaginyl-[protein] + H2O = an oligosaccharide-(1-&gt;3)-[oligosaccharide-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-D-GlcNAc + N(4)-(N-acetyl-beta-D-glucosaminyl)-L-asparaginyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:73067"/>
            <dbReference type="Rhea" id="RHEA-COMP:12603"/>
            <dbReference type="Rhea" id="RHEA-COMP:18176"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:132248"/>
            <dbReference type="ChEBI" id="CHEBI:192714"/>
            <dbReference type="ChEBI" id="CHEBI:192715"/>
            <dbReference type="EC" id="3.2.1.96"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 85 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.96" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPX9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013062"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013222.1">
        <property type="protein sequence ID" value="ENSXCOP00000013062.1"/>
        <property type="gene ID" value="ENSXCOG00000009880.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018512"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033925">
        <property type="term" value="F:mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06547">
        <property type="entry name" value="GH85_ENGase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000043">
        <property type="entry name" value="cytosolic endo-beta-N-acetylglucosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032979">
        <property type="entry name" value="ENGase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057882">
        <property type="entry name" value="ENGase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005201">
        <property type="entry name" value="TIM_ENGase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13246:SF1">
        <property type="entry name" value="CYTOSOLIC ENDO-BETA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13246">
        <property type="entry name" value="ENDO BETA N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03644">
        <property type="entry name" value="Glyco_hydro_85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25529">
        <property type="entry name" value="Ig_ENGASE1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytosolic endo-beta-N-acetylglucosaminidase TIM barrel" evidence="10">
        <location>
            <begin position="125"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Cytosolic endo-beta-N-acetylglucosaminidase C-terminal" evidence="11">
        <location>
            <begin position="600"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="721" mass="81575" checksum="8892A47C3E32DCD4" modified="2018-12-05" version="1">KETVQFPGKTSSYANRLKLSLDSFRLKQTDKNDAVVTESCFDQPVDSSVHEVIKYSPSALPAKHYDADTTEPISCGLSSMNDLLSWNRSEANPFNVAAVPLAPRKPRLVNCLRRTLVSHDMMGGYLDDRFVQGTSSEEPYAFYHWQYIDIFNYFTHQMVTVPPAVWTNAAHKHGVIVLGTFITEWTDGAAVCEAFLKDEESYRAVADKLVQISHYYGFDGWLINIENTLSEGAVKNAPLFLRYLMEQMHERVPSSLILWYDSVTENGKLQWQNELNQSNRIFFDACDGFFTNYNWTEENLEWMKDYSVVQGRQADVYIGVDVFARGKVVGGMLETNKALDVIRKHNFSAAIFAPGWVYESQSDKSKFRTHQDKFWALLSDYLYIHRPASQLPFISSFCQGFGKAVYREGQHETAKRWFNLAAQEIQPLYYHMALEDGGWVRSSGCPEDAWNGGCSLLLDGLIPSTLNTPICAKIFSLHVPLDSRIMVTLVYKASAGITVSLQLRTTDSSHCSHTDVHEVTLNSVTPETVGSGSQLESQMSELCGNRDLHGWTVSRCSQLELHGCVLREVCVLIHREGEPQDTTFSCRVGEIKVLDVGTLQPPTDPVRGLCIDDVVWLRGAGSSPESSSSCLRLNATLRWDYPVESVRHFRVHWRRLRGPDPRIPPGQLELLARAYSNQFRVTELLVPKPPSVLELLVEPVTRWGSLVPESRWGRRQLSYTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LQC8</accession>
    <name>A0A3B5LQC8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LQC8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012985"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013145.1">
        <property type="protein sequence ID" value="ENSXCOP00000012985.1"/>
        <property type="gene ID" value="ENSXCOG00000009827.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005602"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006536">
        <property type="term" value="P:glutamate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342:SF0">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="39"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="414" mass="45078" checksum="D25081388D4D670F" modified="2018-12-05" version="1">MSKINSGSTGCRAVVMAGKYLSGPGRCSAVLAGTSGRQRGQMCADQRRTGSSTAARMGGRTAAAGQQESPGYFSAADRHALANRNLIYRDVKAFLSEVGGDPREARYWLAQFQRANLSQSPAFAVLEVDDLVFQSRETVQSLAFGLSFLQRMDMKLVVVMGCSYNQESGSWCTRGLVERCQQLTEALQLHSATVLPFFSAESFLQLHEAPQGSSAPHSISVDISLLQWSLDCGTIPLVCPVGRDGRGCSLVLDPTEVTAAISRALQPLKVMFLNNSGGLRGEKHKVLDTVSLPTDLPGLSAAEWLGQVERRRLTTIARLLNQLPTESSAVITSADTLLTELFSHRGEASEHKAKAKHVPLSHSVSDCFEKRQQSERSCRILWSPLVDMEDFFLFHSLAIGFGFSRNNVLQCCST</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LR01</accession>
    <name>A0A3B5LR01_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Solute carrier family 47 member 3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LR01"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013225"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013388.1">
        <property type="protein sequence ID" value="ENSXCOP00000013225.1"/>
        <property type="gene ID" value="ENSXCOG00000010024.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163234"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="30840" checksum="D236911B0C261AED" modified="2018-12-05" version="1">DCRWWVESIRLNFFKIKLCPFCTKLTMYNMHLTSFHLTSFLLSYLGWSKECLVDWKSYIQLAVPGMVMMCVEWWTYEIGGFLAGLISEVELGTQSVVYQLANIAYMFPLGFSVAGNVRVGNALGAGETEQAKLPLGMLLFTTGSVSNLPLLEGCVTSLVVKSNLFFYTSHDQAASGGIIRGAGKQRVGAICYFLGFYGIGFPIGIPLMFVAKQGIKGAQSEDAEHSLSVTDAMDLVDTEEVSLEVQPSFRSLVLRRGLALAGMLLVLATGITINLLVTNLVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LR46</accession>
    <name>A0A3B5LR46_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Myosin tail domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LR46"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014563"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014748.1">
        <property type="protein sequence ID" value="ENSXCOP00000014563.1"/>
        <property type="gene ID" value="ENSXCOG00000011047.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164626"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002928">
        <property type="entry name" value="Myosin_tail"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349">
        <property type="entry name" value="CINGULIN-LIKE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46349:SF6">
        <property type="entry name" value="MYOSIN-6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01576">
        <property type="entry name" value="Myosin_tail_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="5" id="KW-0518">Myosin</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0787">Thick filament</keyword>
    <feature type="domain" description="Myosin tail" evidence="9">
        <location>
            <begin position="5"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="79"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="95"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="141" mass="16566" checksum="D714B1BBC33C7571" modified="2018-12-05" version="1">MKGGKKQLQKLEQRVRELETEVEGEQKRGADAVKGVRKYERRVKELTYQTEEDKKNITRLQDLVDKLQLKVKAYKRQAEEAEEQANSHMSRLRKVQHEMEEAQERADIAESQVNKLRAKSRDVGKVPIWTILSVNYTLEPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LS66</accession>
    <name>A0A3B5LS66_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LS66"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010654"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010778.1">
        <property type="protein sequence ID" value="ENSXCOP00000010654.1"/>
        <property type="gene ID" value="ENSXCOG00000008052.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286959"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048619">
        <property type="term" value="P:embryonic hindgut morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009880">
        <property type="term" value="P:embryonic pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001732">
        <property type="entry name" value="Zgc:162936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF10">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="31"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="107" mass="12578" checksum="67F82B519C83DA32" modified="2018-12-05" version="1">MTLILEEPELLQLKEEDGRAKGKRHKVGNRAFCQICGKSFNQFYLYNHIRTHTGEKRFSCKICGKGFIQSYQYTVHMRTHTGERPFSCHICGKRFVQKNNLQKKTHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTA3</accession>
    <name>A0A3B5LTA3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SEFIR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTA3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011039"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011166.1">
        <property type="protein sequence ID" value="ENSXCOP00000011039.1"/>
        <property type="gene ID" value="ENSXCOG00000008340.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000170852"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006959">
        <property type="term" value="P:humoral immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053047">
        <property type="entry name" value="E3_ubiq_ligase_TRAF3IP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013568">
        <property type="entry name" value="SEFIR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34257">
        <property type="entry name" value="ADAPTER PROTEIN CIKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34257:SF3">
        <property type="entry name" value="ADAPTER PROTEIN CIKS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08357">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51534">
        <property type="entry name" value="SEFIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEFIR" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="108" mass="12803" checksum="5B849ADE349C45BF" modified="2018-12-05" version="1">YLIIIIISPKYYETVTTSPFDLENDERTFNTVYIHKQNEFIQNGSKNFRFIPILFPGAKKCHVPNWLQNTHVYGWPRDRDDILRRLMRVEKYNPPPIGELPTIVSIPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTR0</accession>
    <name>A0A3B5LTR0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cysteine-rich protein 2-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ADA2A-containing complex subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">CRP2-binding partner</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Lysine acetyltransferase 14</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. May function as a scaffold for the ATAC complex to promote ATAC complex stability. Has also weak histone acetyltransferase activity toward histone H4. Required for the normal progression through G1 and G2/M phases of the cell cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with the LIM 1 domain of CSRP2. Component of the ADA2A-containing complex (ATAC), composed of CSRP2BP, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1. In the complex, it probably interacts directly with KAT2A, MBIP and WDR5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTR0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014235"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014412.1">
        <property type="protein sequence ID" value="ENSXCOP00000014235.1"/>
        <property type="gene ID" value="ENSXCOG00000010776.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001146"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140672">
        <property type="term" value="C:ATAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051302">
        <property type="term" value="P:regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000013">
        <property type="entry name" value="cysteine-rich protein 2-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.980.20:FF:000004">
        <property type="entry name" value="Cysteine-rich protein 2-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.980.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916">
        <property type="entry name" value="CYSTEINE AND GLYCINE-RICH PROTEIN 2 BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20916:SF26">
        <property type="entry name" value="CYSTEINE-RICH PROTEIN 2-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="14">
        <location>
            <begin position="540"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="180"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="284"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="204"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="684" mass="77230" checksum="017A92DE5BABE499" modified="2018-12-05" version="1">EGLEEGEVEGETLLIVESEDQEDLSHDQSGDSLTSDLGEDADGGWACEDMSFYCDRCHKWVPAQLRGDQPSYLKGDNFFKFLCCDCSEDGKESFERLRLTWQQVVMLAMYNLSLEGTGRQGYFRWKEDICAFIGRHWNFLLGNKKTSTWWSTVAGCLSVGSPAFFRSGAQEFGEPGWWKLVQNRPPTLRPDGDKSGTKTKSGVRRPDLVLERGEVVDFSSLSSSDQTPLTSPSPSPSPDFSGPGTPASHSATPSLLSEVDLIPDAMPPQALFHDDEELETEGMIDPGMEYLPPPSASHTGQKKLRPPHTLIKREAESEEDDAHEERFEQPAGCGEAPPPDRRVAERRRMNLQDKLDGAAPRPVVSALSLYDERLLLRRLDGCPLALAVTPHAKRLRRKLLVRHAKRQRGLPLLDIDRAVSAALSLVGGIYATQEAEPRPVGGVKRKYCTSSQEQRILDRFQTSASSRPGVQQRSVSFWHRLMGAEGGLDQNIKSPYTARILKPFIRDFESRPLKLRLLAEIRAFPHRTDPDWTPEPDAPIDYCYVRPNHIPSVNAMCHDSFWPVDLSECLQYPDFSVVALYRKVVVGFGFMVPDVKYSEAYISFLLVHPEWRRAGIGTFMIYHLIQQTCMGKDVTLHVSASNPAMLLYQKFGFKAEEYILDFYDKYYPVDSSECRHAFFLRLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTR6</accession>
    <name>A0A3B5LTR6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">General transcription and DNA repair factor IIH helicase subunit XPD</fullName>
            <ecNumber evidence="18">5.6.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">CXPD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">DNA 5'-3' helicase XPD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">DNA excision repair protein ERCC-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">DNA repair protein complementing XP-D cells</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Xeroderma pigmentosum group D-complementing protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="27 28">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="27" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the helicase family. RAD3/XPD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTR6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012716"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012870.1">
        <property type="protein sequence ID" value="ENSXCOP00000012716.1"/>
        <property type="gene ID" value="ENSXCOG00000009604.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182970"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061031">
        <property type="term" value="P:endodermal digestive tract morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035315">
        <property type="term" value="P:hair cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045951">
        <property type="term" value="P:positive regulation of mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18788">
        <property type="entry name" value="SF2_C_XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000135">
        <property type="entry name" value="DNA repair helicase RAD3, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000128">
        <property type="entry name" value="Putative DNA repair helicase RAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010643">
        <property type="entry name" value="HBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013020">
        <property type="entry name" value="Rad3/Chl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001945">
        <property type="entry name" value="RAD3/XPD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00604">
        <property type="entry name" value="rad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06777">
        <property type="entry name" value="HBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00852">
        <property type="entry name" value="XRODRMPGMNTD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="25" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="26">
        <location>
            <begin position="7"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="224"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00078828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00079246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00081072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00081188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00082576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="735" mass="84096" checksum="5DE66F335C5524CE" modified="2018-12-05" version="1">FRLNIEGLLVYFPYDYIYPEQYSYMLELKRTLDAKGHGVLEMPSGTGKTISLLSLIVVVEELRKLMEFYAKETGEVNNFLALSLSSRKNLCIHPEVSSLRFGKEVDGKCHSLTASYIRAQRHSNPNQPVCRFYEEFDAVGRQVPLPAGIYNLDDLKDFGRRKGWCPYYLARYAILHANIVVYSYHYLLDPKIADLVSKELAKKSVVVFDEAHNIDNVCIDSMSVNITRRMLDRCQNNVDTLQNTIHKIKETDAAKLREEYRRLVEGLKEANVARETDIYLANPVLPDEVLQEAVPGSIRTAEHFVGFLRRFLEYLKARLRVQHVVQESAPQFLKDIFDKVCIDRKPLRFCAERLQSLLRTLEIADIADFSAVTLISNFATLVSTYSQGFTIIIEPFEDRTPTIANPVLHFSCMDPSIAIKPVFQRFQSVIITSGTLSPLDIYPRILDFRPVTMASFTMTLARTCLCPLIVGRGNDQVALSSKFETREDFAVIRNYGNLLLEMSAVVPDGIVAFFTSYVYMENIVASWYEQGILENIQKNKLIFIETQDAAETSMALEKYQEACENGRGAILLSVARGKVSEGIDFVHHFGRAVIMFGVPYVYTQSRILKARLEYLRDQFQIRENDFLTFDAMRHAAQCVGRAIRGKTDYGLMIFADKRYARSDKRGKLPRWIQEHINDGSLNLTVDEAVQLSKHFLRQMAQPFRQEDQLGLSLLTLEQLQSEEMLQKISQMAHQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LU11</accession>
    <name>A0A3B5LU11_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transgelin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the calponin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LU11"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012806"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012960.1">
        <property type="protein sequence ID" value="ENSXCOP00000012806.1"/>
        <property type="gene ID" value="ENSXCOG00000009680.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159385"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21281">
        <property type="entry name" value="CH_TAGLN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000039">
        <property type="entry name" value="Transgelin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000557">
        <property type="entry name" value="Calponin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF10">
        <property type="entry name" value="TRANSGELIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00402">
        <property type="entry name" value="Calponin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00890">
        <property type="entry name" value="TRANSGELIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01052">
        <property type="entry name" value="CALPONIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51122">
        <property type="entry name" value="CALPONIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="4">
        <location>
            <begin position="32"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="207" mass="23486" checksum="0D736D891E600A28" modified="2018-12-05" version="1">EAAQMKPAMANRGPSYGLSREVQEKIEQKYDPDLEQRLVDWIVAQCGGNLEKPQPGKQNFQTWLMDGTILCRLINSLYPRGKEPIKKIAETQMAFKQMEKISQFLQAAEAYGVTTTDIFQTVDLWEGKDLAAVQRTLMALGSLAVTKDDGQYKGDRDWFHRKAQGYRREFTEEQLRQGQSLISLQMGSNRGASQAGMTGYGMHRQIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUA3</accession>
    <name>A0A3B5LUA3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thromboxane A2 receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Prostanoid TP receptor</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027898996.1">
        <property type="nucleotide sequence ID" value="XM_028043195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUA3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014430"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014614.1">
        <property type="protein sequence ID" value="ENSXCOP00000014430.1"/>
        <property type="gene ID" value="ENSXCOG00000010941.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114160542"/>
    <dbReference type="KEGG" id="xco:114160542"/>
    <dbReference type="CTD" id="100003535"/>
    <dbReference type="GeneTree" id="ENSGT01030000234559"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004957">
        <property type="term" value="F:prostaglandin E receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004958">
        <property type="term" value="F:prostaglandin F receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004960">
        <property type="term" value="F:thromboxane receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000163">
        <property type="entry name" value="Thromboxane A2 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008365">
        <property type="entry name" value="Prostanoid_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR FAMILY 1 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11866:SF33">
        <property type="entry name" value="THROMBOXANE A2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01788">
        <property type="entry name" value="PROSTANOIDR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="153"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="197"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="44"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="353" mass="38673" checksum="79B22E867CBB0AF0" modified="2018-12-05" version="1">MTTPSVPINVSFPNVSATNTTSPSVSKPDGLGMSLFTMLFGAISNLTALGILARSRGRFRRQSKKSFLLLTVALLVADLLGHVILGAFALHVHLNQGVLQTGEPFCNVFGASMVFFGLCSLFFGFAMAVERCVAITQPFFHVSMVTAAHAKSVVLFLSTFALVLALSPFFSTGRYILQFPKTWCFLPIHGSADTTLVLVFSSLGLFALFFSLFCNIVSGLALLHARIKSRNGNTTPSGSSDRRASTASSASLFCTLDVEMMVQLAAVTVVSCVCWGPFLIHIFMKQFKMHTEPDLLVLIGLRMAAWNQILDPWVYILLRRKMLTRLCCGRYYQGIPTSSNSLLESRRNTINLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUE8</accession>
    <name>A0A3B5LUE8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ephrin-B1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 14">Belongs to the ephrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUE8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014767"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014952.1">
        <property type="protein sequence ID" value="ENSXCOP00000014767.1"/>
        <property type="gene ID" value="ENSXCOG00000011181.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160128"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046875">
        <property type="term" value="F:ephrin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000027">
        <property type="entry name" value="ephrin-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031328">
        <property type="entry name" value="Ephrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019765">
        <property type="entry name" value="Ephrin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001799">
        <property type="entry name" value="Ephrin_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304">
        <property type="entry name" value="EPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304:SF17">
        <property type="entry name" value="EPHRIN-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00812">
        <property type="entry name" value="Ephrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01347">
        <property type="entry name" value="EPHRIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01299">
        <property type="entry name" value="EPHRIN_RBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51551">
        <property type="entry name" value="EPHRIN_RBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Ephrin RBD" evidence="17">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="143"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="223"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="225"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="13">
        <location>
            <begin position="66"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="298" mass="32822" checksum="812D068A453712A0" modified="2018-12-05" version="1">TGHPFYLEVTICLFFLPPPRFLPGKGLVIYPDIGDKLDIICPSADQGRDYEFYKLYLVKREQAESCSTVLDPNVLVNCNKPGKNIKFTIKFQEFSPNYMGLEFKRNVNYYITSTSNGTIEGLENREGGVCKSRAMKVIMKVGQETSSSGLPDPSKTGPVMPGIPGERSHTHPNDPGSSSSTDGFLSSKVAVFSSIAIGCIIFLVLILLLVILLIKMRKRSRKNAHSQARPSALSLSTLSNPKLPGGTAGTEPSDIIIPLRTENNYCPHYEKVSGDYGHPVYIVQEMPPQSPANIYYKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUQ3</accession>
    <name>A0A3B5LUQ3_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FH2 domain containing 3</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUQ3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015723"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015917.1">
        <property type="protein sequence ID" value="ENSXCOP00000015723.1"/>
        <property type="gene ID" value="ENSXCOG00000011879.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155128"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46345:SF7">
        <property type="entry name" value="FH2 DOMAIN CONTAINING 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46345">
        <property type="entry name" value="INVERTED FORMIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="2">
        <location>
            <begin position="8"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="497"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="553"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="683" mass="77150" checksum="F194E4A32A4DF761" modified="2018-12-05" version="1">MKQYIKCDPFLQTALRSMKKLNWDTLPNHHVLGKLNVWTSKRTQRDLVLDIQTMEELFSHDDKSASLRASKVFSLSKSDGMNLCGQESQVTNRYVQIRNRLSMQALLSFSFFMSLPVSEIVEDIRQGNWQRFGNGKLKDLCKLLPEESEVTGTLFLLIFHAYIDSKQKYGRAMVLWNSFYGERLKVMLLREEFFPLMEEVKNAIAVMIKAANELLDCDDLHSVIRLVLKAGNYMNAGGYSANAIGFRVASLLNLADTKANKPGMNLMHYVAKFVSEFYQRICKDDVIKDLKEEIQKMEEVKLFSSIHPGLLQQIDQFLKRADTLLADVDLSLNELKALSDAVAGYFCEDPATFKLEECCSIFYSFCKRFDKAVQENREREAVEHRHKRSESIQLVVKRRSVASCSALDPTDDSCLESALHNLLSKPPSGLSRCRKNYQPQAKGLRSESCSQTSQKEMPILCKTTDSPEKKQTKLLKDVELAKLEEEEVLCYQNVKSNRNDEKVSGSPQHSKGEQDEASTPRTPSPRTRDYFFTSNGNMGSPWTILSPISCSPRSNSLRGKARRGSSTSSGNDPDDGVWEIDETNLKNYSSQESLTSPSGGSASLPECIGQRALSQGPLLRSASVDQTLRSPGMGFRLRYLFQRNVKERSDRKVENYAEGSSGLKSLFKLIGSRSKLCNLDEKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUR4</accession>
    <name>A0A3B5LUR4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IL-17 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUR4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011574"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011706.1">
        <property type="protein sequence ID" value="ENSXCOP00000011574.1"/>
        <property type="gene ID" value="ENSXCOG00000008709.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00740000116886"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010345">
        <property type="entry name" value="IL-17_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06083">
        <property type="entry name" value="IL17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017420409" evidence="5">
        <location>
            <begin position="24"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="146" mass="16394" checksum="45C85BD05CFCA07E" modified="2018-12-05" version="1">MEKFQVRNVPLCIFLLILHQCVAMPLSNQCIGETLCTYTLKDSYSQMTNLPGDINTRSLATWVYQKKTDLTREHQVIYEARCQDSHFGGSCNSALGLETIPVTLRIPVLRKNSACYPLPSYSVEFENITVACICALARTQEEIAQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUZ6</accession>
    <name>A0A3B5LUZ6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUZ6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014957"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015145.1">
        <property type="protein sequence ID" value="ENSXCOP00000014957.1"/>
        <property type="gene ID" value="ENSXCOG00000011339.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271858"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01852">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000366">
        <property type="entry name" value="GTPase, IMAP family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF112">
        <property type="entry name" value="SI:CH211-113E8.5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="5">
        <location>
            <begin position="12"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="207"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="270" mass="31018" checksum="CBBEC1BF471A1D7C" modified="2018-12-05" version="1">LTSYKSTNFTNNNELRIVLVGKTGSGKSASGNTILGRKCFDSKCSPRSLTINCSKCNSELDGQQVAVIDTPGLSDTRFDEEKTVKDFSRCVPFAAPGPHVFLLVIALNRFTEEEKKSVQKIQEIFGKTADKYSMVLFTHGDLLEDTSIENFLSESSELQDLVRRCNGQYHVFNNKLENKKPQVTELLQKIRTIVDKNGGSHYTNEMFQEAERIIAVKKQQILKENEEKMRKERAELEEEIQLQYQKEMQKINELFEAERARERQERHVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVQ1</accession>
    <name>A0A3B5LVQ1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NOP2/Sun RNA methyltransferase 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NSUN6</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVQ1"/>
    <dbReference type="SMR" id="A0A3B5LVQ1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015232"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015423.1">
        <property type="protein sequence ID" value="ENSXCOP00000015232.1"/>
        <property type="gene ID" value="ENSXCOG00000011542.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155370"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21150">
        <property type="entry name" value="PUA_NSun6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF34">
        <property type="entry name" value="TRNA (CYTOSINE(72)-C(5))-METHYLTRANSFERASE NSUN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="7">
        <location>
            <begin position="138"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="239"/>
            <end position="245"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="454" mass="49577" checksum="4A0B55F658DA1D5F" modified="2018-12-05" version="1">MSVFPKISLKPEVTEYLRNVFLNKEVTAVDTQEAESRFQKLLTCLSHPPSSTCVRASTHLSQMRHLFLFLKQQKCGSSAEVPSVQIVSHPRITDVLLLPVDGPRYRLSSELVVGAQCGSAVLRGAHVFAPGIVASPKYMKAGDMVSVFSDLEGRCTRGATSFQGNKVFVGNGVAEMDRSAIFATETPARGVGVRMVDPLYKSPSFDGVLPSMVFLQNLPSVVVGHVLTPQPGERILDMCAAPGGKTCHIAALMGDKGEVVALDRIRNKIDRIRQNAQMLSLQSIKAFCFNSTQAVSDEPKQEAEGPPFPPESFDRVLLDAPCSGLGQRPNMASTWSLKEFCSYPPLQRKLFQAAVRLLKKGGILVYSTCTVTLSENEEQVAWALNTFPCLSLQPQEPHIGSEGMLGAGLSPEQLRLLQRFSPELSWEQTDTTAPLTCRADTDTIGFFIAKFLKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWW8</accession>
    <name>A0A3B5LWW8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWW8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013826"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013995.1">
        <property type="protein sequence ID" value="ENSXCOP00000013826.1"/>
        <property type="gene ID" value="ENSXCOG00000010467.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162649"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF319">
        <property type="entry name" value="CYTOCHROME P450, FAMILY 2, SUBFAMILY AC, POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="157" mass="17948" checksum="88A116F3ED8294D1" modified="2018-12-05" version="1">MVHVEDRKHLPYTDAVIHEIQRLCNISPMAVPHKTSRDVTFHGYFIKKGTTVIPLLTSVLYDESEWESPHTFNPSHFLDKEGRFIKRDAFLPFSAGRRVCLGESLARMEIFLFFTSLLQHFRFIPPPGVSEDDLDLTPAVGFTIPPSPHELCAISRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LXH1</accession>
    <name>A0A3B5LXH1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Motile sperm domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a role in differentiation and/or proliferation of mesenchymal stem cells. Proposed to be involved in epithelial-to-mesenchymal transition (EMT). However, another study suggests that it is not required for EMT or stem cell self-renewal and acts during later stages of differentiation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027864571.1">
        <property type="nucleotide sequence ID" value="XM_028008770.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027864572.1">
        <property type="nucleotide sequence ID" value="XM_028008771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LXH1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015605"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015798.1">
        <property type="protein sequence ID" value="ENSXCOP00000015605.1"/>
        <property type="gene ID" value="ENSXCOG00000011793.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114139108"/>
    <dbReference type="KEGG" id="xco:114139108"/>
    <dbReference type="CTD" id="56180"/>
    <dbReference type="GeneTree" id="ENSGT00940000155266"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000431">
        <property type="entry name" value="motile sperm domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039283">
        <property type="entry name" value="MOSPD1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34441:SF1">
        <property type="entry name" value="MOTILE SPERM DOMAIN-CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34441">
        <property type="entry name" value="MOTILE SPERM DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="188"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="MSP" evidence="12">
        <location>
            <begin position="12"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="113"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="214" mass="24040" checksum="E129091E409C6EB8" modified="2018-12-05" version="1">MQQHQLAEGNLPVFVFPTELVFYADEQSSHKQVLTLYNPYEFALKFKVLCTAPNKYTVVDSTGAVKPQCCVDIVIRHRDVRPCHYGAYDKFRLQVSEQSQRKALGRKEVTATLRPSASQEPSSARSQDEERRIKEQFADSEFFEQTAFQTESRPVAGGPSLLTVLLGLVCMIALMLPTLGEQESTVPVYLHLSVNKKLVAAYVLGLLTMVILRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LYB8</accession>
    <name>A0A3B5LYB8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exosome complex component RRP45</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Exosome component 9</fullName>
        </alternativeName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000015915"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016112.1">
        <property type="protein sequence ID" value="ENSXCOP00000015915.1"/>
        <property type="gene ID" value="ENSXCOG00000011971.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183130"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11368">
        <property type="entry name" value="RNase_PH_RRP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033100">
        <property type="entry name" value="Rrp45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF14">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="11">
        <location>
            <begin position="49"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="12">
        <location>
            <begin position="181"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="410" mass="46157" checksum="8C85AABD98A13378" modified="2018-12-05" version="1">MKDTPMSNCERDFILKAIEEKKRLDGRQTYDYRKIKITFGTDYGCLCGQVMAQVSCELVVPKENRPNEGIMFFNLELSPMASPAFEPPGRAELLKCLSRAPHRAYETTRKSLVLCVTIVQVWQIRVDVHTLNNDGNLMDAASVAAIGALCHFRRPDVSIQGEEVTVYSPEERDPIPLSIYHMPISVSFAFFQQGTFLLVDPCEREERVMDGLLMIAMNKHREICSIQSSGGIMLLKEQASRLERXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVDSSQVLEQIIRFSFGFKGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZ20</accession>
    <name>A0A3B5LZ20_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZ20"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017353.1">
        <property type="protein sequence ID" value="ENSXCOP00000017133.1"/>
        <property type="gene ID" value="ENSXCOG00000012918.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286959"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001732">
        <property type="entry name" value="Zgc:162936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002716">
        <property type="entry name" value="Zinc finger protein 212"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF142">
        <property type="entry name" value="ZINC FINGER PROTEIN 384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="334"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="362"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="390"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="418"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="446"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="474"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="502"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="530"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="558"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="586"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="73"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="109"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="619" mass="70451" checksum="5725EE18293A67A4" modified="2018-12-05" version="1">MQSNQLRVEEVESDNIVEREFQPPTPPKRKKKKVSFAVWWELHAPEELKISAESIEQQDASLVVENESITKALETVDQKGDRQPPESESNEKNQVPFKTWSEEHVPSEQEISAGNTEKQNDSLVSEEESTAGVQETIDVAEKEQPSKSKKQPPTTRRNRKKMIPFQVWLEKHAPADLRISAEGTGMENDSLVTKQESISVLQETPEKEQPSTSGTENVKKQDTSLPEEGNQEDSLKKICKEEPVVLISTTQLVTEKNNISKLQKKEDQKGEKQLSKTKRIKKTPLLWKTFPESTLDQEELESLQDLKHQQIKLEEEEVYSAEAEKKKQERRKPFSSVICEKRLTFKSVFDAHMRTHTGEKPFTCVTCGKSFCQKSNLPRHMMIHTGEKPFSCVTCGKSFTQKHVLTKHMMIHTGEKPFSCVTCGKSFTQKHVLTKHMMIHTGEKPFSCVTCGKSFTQKHVLTKHMMIHTGEKPFSCVTCGKSFTQKHVLTKHMMIHTGEKPFSCVTCGKSFTQKHVLTKHMMIHTGEKPFSCVTCGKSFTQKHVLTKHMMIHTGEKPFSCVTCGKSFTQKHVLTKHMMIHTGEKPFSCVTCGKSFTQKHVLDQNQKPGKIDTESKRTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M090</accession>
    <name>A0A3B5M090_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glia maturation factor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the actin-binding proteins ADF family. GMF subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M090"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013409"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013574.1">
        <property type="protein sequence ID" value="ENSXCOP00000013409.1"/>
        <property type="gene ID" value="ENSXCOG00000010117.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008920"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071846">
        <property type="term" value="P:actin filament debranching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034316">
        <property type="term" value="P:negative regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011171">
        <property type="entry name" value="GMF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11249:SF4">
        <property type="entry name" value="GLIA MATURATION FACTOR GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11249">
        <property type="entry name" value="GLIAL FACTOR NATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001788">
        <property type="entry name" value="GMF-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0339">Growth factor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="3">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR001788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="104" mass="12277" checksum="CDE5B45C40430A3B" modified="2018-12-05" version="1">MYSCNLLREKLKKFRFRKETNNAAILIKIDMAKQLVILEEEYEDISLDDLRNELPERQPRYPESRHYKYTHADGRVSYPLCFIFSSPMGNNDTNLAHPAQVLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0H2</accession>
    <name>A0A3B5M0H2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">LIM domain kinase 1</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0H2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016907"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017124.1">
        <property type="protein sequence ID" value="ENSXCOP00000016907.1"/>
        <property type="gene ID" value="ENSXCOG00000012749.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156345"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051496">
        <property type="term" value="P:positive regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09462">
        <property type="entry name" value="LIM1_LIMK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000282">
        <property type="entry name" value="LIM domain kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000082">
        <property type="entry name" value="LIM domain kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000038">
        <property type="entry name" value="LIM domain kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050940">
        <property type="entry name" value="Actin_reg-Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485">
        <property type="entry name" value="LIM DOMAIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485:SF7">
        <property type="entry name" value="LIM DOMAIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="14 20" id="KW-0440">LIM domain</keyword>
    <keyword evidence="8 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 20" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="23">
        <location>
            <begin position="25"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="224"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00040667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="537" mass="61238" checksum="E38A3A48C7E211DC" modified="2018-12-05" version="1">MRLMLLCCTWKDERMGEEEAGGRLPECAGCKERIYDEQYLQALNADWHTICFRCCKCSASLSHWYYEKDGRLYCKKDYWAKFGELCHGCNDPITTGLIMVRAASSLSAPCPLLALCLLMKLKLHLSPHILVAPCFTCSLANSPKHTDRSFKISSSTQIRLNGSSRELQQLSLSLVSLRRSCSIDKSPGSSNAASPVSHRKDINRSESLRVVSNRTHRIFRPSDLIHGEVLGKGCFGQAIKVTHKETGEVMVMKELIRFDDETQRTFLKEVKVMRCLDHPNVLKFIGVLYKDKRLNFIAEYIKGGTLREIIKKMDNNFPWNQRVSFAKDIAAGMTYLHSMNIIHRDLNSHNCLVREDHTLVVADFGLARLMVDDKCENKLTQGKLSGLKKPDRRKRYTVVGNPYWMAPEMIHGMIYSRTCGNLNNPDFLCIIGRVNADPDYLPRAMDFGLNISGFLENYYPPNCPPAFFPIAALCCDLDADKRPAFSKLEEWLENLKMHLDIGLPLMSELDQLHKAFWEHHSIPRPDNGLHTHPEQPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M0P7</accession>
    <name>A0A3B5M0P7_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M0P7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016977"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017196.1">
        <property type="protein sequence ID" value="ENSXCOP00000016977.1"/>
        <property type="gene ID" value="ENSXCOG00000012800.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000174814"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="103" mass="11072" checksum="BEEFC6148F5DB87A" modified="2025-10-08" version="2">MPSSLPPLPLYEQPPISPFTSPETSTPPPLPRSPSTALNGEHKSGRSPVSPGFPQTQPLRFTAEGGGPGKLRPVRAAPPPPKQQPRRPHEKSDKTEEVEITLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M1V8</accession>
    <name>A0A3B5M1V8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Osteocrin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M1V8"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017608.1">
        <property type="protein sequence ID" value="ENSXCOP00000017387.1"/>
        <property type="gene ID" value="ENSXCOG00000013106.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000171805"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021088">
        <property type="entry name" value="Osteocrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35353">
        <property type="entry name" value="OSTEOCRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35353:SF1">
        <property type="entry name" value="OSTEOCRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11037">
        <property type="entry name" value="Musclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="13693" checksum="D7F18BD91474030A" modified="2018-12-05" version="1">AVNFCLCNQHLYLSHIDGAGLRTLAVLWRHPGRMKPRLELSAKLLQTDNLVMMMENDVTEPKRKRSFPGNNAPLDRLSMSSMETRQGKKKQSKVVESPRRRNNLPPIDRVGMSRLPTTRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M271</accession>
    <name>A0A3B5M271_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M271"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017497"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017719.1">
        <property type="protein sequence ID" value="ENSXCOP00000017497.1"/>
        <property type="gene ID" value="ENSXCOG00000013197.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155905"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20482">
        <property type="entry name" value="CC_brat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000032">
        <property type="entry name" value="Tripartite motif containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000154">
        <property type="entry name" value="Tripartite motif-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003649">
        <property type="entry name" value="Bbox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017868">
        <property type="entry name" value="Filamin/ABP280_repeat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001298">
        <property type="entry name" value="Filamin/ABP280_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047153">
        <property type="entry name" value="TRIM45/56/19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462">
        <property type="entry name" value="BONUS, ISOFORM C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25462:SF296">
        <property type="entry name" value="MEIOTIC P26, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00630">
        <property type="entry name" value="Filamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00502">
        <property type="entry name" value="BBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00557">
        <property type="entry name" value="IG_FLMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50194">
        <property type="entry name" value="FILAMIN_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51125">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="15">
        <location>
            <begin position="23"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="16">
        <location>
            <begin position="113"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="Filamin" evidence="11">
        <location>
            <begin position="320"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="12">
        <location>
            <begin position="470"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="12">
        <location>
            <begin position="517"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="12">
        <location>
            <begin position="561"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="424"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="674" mass="74501" checksum="9194BA065BFA8CB0" modified="2018-12-05" version="1">MATESSTIPSPVVRQIDKQFLICSICLDRYQNPKVLPCLHTFCERCLQNYIPAHSLTLSCPVCRQTSILPERGVAALQNNFFITNLMDVLQHRPDTCSQEATTLSNITIVATGQLLSCPNHGGSVMEFYCPPCETAMCQECTSGEHREHPTVPLKDVVEQHKTSLHDQLNAVKKRLPEIDSALLLLSEILQQLTNQKSSIEEDIHKTFDELQKTLNVRKSVLLMELEVNYALKQKVLQAQLDTLLQGQEGISSSCNFTEQALSHGTEAEVLLVKKQMAERLVELANQELPLQPGENCQLDFLVETDGLKKSIHNLGTVVTTNAVASETVVTGEGLRHCVVGVPTAVTITTKDKDGELCKMGNAVIAADISSPDGSKVNGEILDNKNGTYEYLFTAPKEGFLYLSLRLYNQHIRGSPFKIKATIHSPNTDGTKKRLKSPGSGHVKQKAIKRPASMYSTGRRKENPIEDDLIFRIGTKGRNKGEFTNLQGVAASSQGKVLIADSNNQCVQIFSNDGQFRSRFGIRGRSPGQMQRPTGVAVHPSGDIIIADYDNKWVSIFSNEGKFKNKVGSGKLMGPKGVAVDRNGHVIVVDNKACCVFIFQANGKLVTKFGNRGNGDRQFAGIPKDRTLRCMWKAACVPKYSEVIHLLSYEGLFRTKTMFCLSHTTKDSYTHTKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2P2</accession>
    <name>A0A3B5M2P2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Solute carrier family 18 member 3a</fullName>
        </submittedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Vesicular transporter family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M2P2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017580"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017803.1">
        <property type="protein sequence ID" value="ENSXCOP00000017580.1"/>
        <property type="gene ID" value="ENSXCOG00000013258.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159449"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005277">
        <property type="term" value="F:acetylcholine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17383">
        <property type="entry name" value="MFS_SLC18A3_VAChT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000109">
        <property type="entry name" value="Putative vesicular acetylcholine transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050930">
        <property type="entry name" value="MFS_Vesicular_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506:SF13">
        <property type="entry name" value="VESICULAR ACETYLCHOLINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="42"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="307"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="363"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="400"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="429"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="43"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="517" mass="56225" checksum="5F334BFA4BC6BE2E" modified="2018-12-05" version="1">MRLTGQQSTNVAQSAASKLSQMGERTKQLGNVIQDPERQKRIILIIVCVALLLDNMLYMVIVPIIPDYLEGLQKAADQEQSAAAPHSNSTNSTIHRAAEGNFDLQIGVLFASKAILQLLVNPLSGTLIDRVGYDIPLFIGLNIMFLSTLTFAFAENYATLFLARSIQGLGSAFADTSGIALIADKFTEESDRSKALGIALAFISFGSLVAPPFGGVLYEFAGKQAPFLILACICFTDGVLCLTVLKPFSNRERENMPVGTPIYKLMIDPYIAVVAGALIVCNIPLAFLEPTIANWMEENMNASEWEIGMTWFPAFFPHVLGVYLTVKLAAKYPHLQWFYGAIGMVFIGASSCTVPACKNFGQLMIPLCGICFGIAFVDTALLPTLGFLVDVRHVSVYGSVYAIADISYCVAYALGPIVAGQIVHNLGFVQLNLGMGLANVLYAPALLLLKNVTQMKPSFSERNMLLEDGPTGLYDTIKMEQREKKRKGLCTTIDESGIETFVQRSHSEEESSGGEYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2V5</accession>
    <name>A0A3B5M2V5_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SHH signaling and ciliogenesis regulator sdccag8</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M2V5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017660"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017884.1">
        <property type="protein sequence ID" value="ENSXCOP00000017660.1"/>
        <property type="gene ID" value="ENSXCOG00000013315.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164292"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007098">
        <property type="term" value="P:centrosome cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043009">
        <property type="term" value="P:chordate embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035148">
        <property type="term" value="P:tube formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031887">
        <property type="entry name" value="SDCCAG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34343">
        <property type="entry name" value="SEROLOGICALLY DEFINED COLON CANCER ANTIGEN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34343:SF1">
        <property type="entry name" value="SEROLOGICALLY DEFINED COLON CANCER ANTIGEN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15964">
        <property type="entry name" value="CCCAP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="86"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="240"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="293"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="490" mass="55831" checksum="C0BBDDA43DF607F4" modified="2018-12-05" version="1">SERANQSLQQLSGALNQLDPDEEDVRPAAGDVFPEEDKTAWSHKTQSEAGESLLLCQFAKVRLDGRSSLPAFRDLVPMNYDQSEYIQHLEAEVKFCKEELQGLKQRIRVVVVENEKLQSELKSRIADQSLKDYPIHNSMLGGSTHLSQVSLMHHKEECSIFALIISFDLFIRFASVNRKDLSVSQKECEEVKVRLRHKEQQVAEALKADGAARVAGLCLQCAQHEAVVAGTHANLHVQAIDRLTKERDELLAALRAMRASQQEAQQREWSACLQVKQAVEIAEEANLQKTRVCMDLRYQLSNASLKKEEAERELRELKAKTSRQKEKAAQFLPELEAGVYKRLVFKRKNRLTSSFRSFPPVGEMENLMGSQNSLIGKLKEECCKLGAKLEELSHQSRSVWDKTLPQAEGRLTLRVCREDDAEDVCVRQGLQMKRLQQLDRHCQSSAQQVCELLAKQSQLMQERNALSEEMQNLRIEVHTRTHTHDELITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2V8</accession>
    <name>A0A3B5M2V8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Rho GTPase activating protein 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ARHGAP9</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M2V8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018438"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018670.1">
        <property type="protein sequence ID" value="ENSXCOP00000018438.1"/>
        <property type="gene ID" value="ENSXCOG00000013879.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182860"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13233">
        <property type="entry name" value="PH_ARHGAP9-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04403">
        <property type="entry name" value="RhoGAP_ARHGAP27_15_12_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12143">
        <property type="entry name" value="SH3_ARHGAP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000003">
        <property type="entry name" value="Putative rho GTPase-activating protein 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000182">
        <property type="entry name" value="Rho GTPase activating protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000101">
        <property type="entry name" value="Rho GTPase activating protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035465">
        <property type="entry name" value="ARHGAP9_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001605">
        <property type="entry name" value="PH_dom-spectrin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF103">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00683">
        <property type="entry name" value="SPECTRINPH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="33"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="7">
        <location>
            <begin position="294"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="437"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="8">
        <location>
            <begin position="635"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="187"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="322"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="572"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="203"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="222"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="573"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="848" mass="95421" checksum="9E749BFD883886BA" modified="2018-12-05" version="1">MLSGSWRRSVGPTQPVAAPVVNPSAMTVCGVPSGTVVLEAQYDYSYRGSDGRQVCIREGERFILLKKTNTDWWQVRRIGAASKTKPLYVPATYVTEVPITPMPSPQRLVTSVSLHSNMPMLPRVSFSPSFTGTGRNEQSQVNKPMYRSMENLNTYSSAFQRSERNTVVGENHFPTLPLSGFTFTPNSSTSQSSRLMVPGSPAASMTQANSSNPRVVPTITRSQSSSNLPENLNENPYDEVGGGLGSRINYKVPKKSCSQWDMVGASGRNNRLQVREKRLSQQEPPIPAVGQQPLQIMDLWEQYSDPGTGRCYYVNSITKERSWKPPRRARSHPAQAQTLPRDTSHLSLPLSKTGNGTMHKVNTLPPCKSGSNRGDSSNTLSTSALTNTEEQCRNSGSQHDAKQQFCHTQSMILSENGKVLPSIEQCYSPDSDGCTPELEKAGLLNKTKIAEGGRKLRKNWSPSWVVLVGNSLVFFKDPKSQTPSSWKPGNSRPESSVDLRGAKLNWANDLSSKKNVFKLRTITGNEFLLQSETDSLIREWYNTIKNVIDRLDRENPLENVLQYSLRRAGSVEMLDHSGDEDDRRNSRKSFPRSASNLENTERKRVKTRLKKLILKRPPLQALQEKGLIKDQVFGCRLEMLCERERTTVPRFVRLCTEAVEKRGLDTDGIYRVSGNLAVIQKLRFLVDHERALTTDGRFMFPADLVQEEKLNLDESEWEDVHVVTGALKLFFRELPEPLLPYGFFTDIVETVKMTDHMDKVDRLKCLVLNMPPPNHDTLEFMCRHLRRVLEQSDTNRMTTQNIGIVFGPTLMRPECDNGNMAVNMIYQNQAVELILSEFDHIFGTRGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3T0</accession>
    <name>A0A3B5M3T0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">SH3 domain containing GRB2 like 2a, endophilin A1</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the endophilin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3T0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014629"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014815.1">
        <property type="protein sequence ID" value="ENSXCOP00000014629.1"/>
        <property type="gene ID" value="ENSXCOG00000011100.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160529"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016191">
        <property type="term" value="P:synaptic vesicle uncoating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11803">
        <property type="entry name" value="SH3_Endophilin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000053">
        <property type="entry name" value="endophilin-A1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035824">
        <property type="entry name" value="Endophilin_A_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF122">
        <property type="entry name" value="ENDOPHILIN-A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017302172" evidence="9">
        <location>
            <begin position="33"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="BAR" evidence="11">
        <location>
            <begin position="24"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="10">
        <location>
            <begin position="276"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="338" mass="38392" checksum="019D333B4512E218" modified="2018-12-05" version="1">MLNKSSLFVYLPSSFLLFVLQRVSEKVGGAEGTKLDVDFTEMEKRVDTTARAVLDIMTKTTEYLQPNPATRAKMSMMSSMSRMRGQEKGPGYTQTETILGESMQRFGRELGEDSNFGLVLIDVGEAMRELGEVKDALDMEVKQNFIDPMQNLHEKDLREIQHHLKKLEGRRLDFDYKKKRQAKVTEDELKAALEKFDDSKEVAEQSMFNLLESDIEQVSQLAALVHAQLQYHTRSAEILTQLSSKIDEQSVPAKEFIPKPRTSLDFTAISKTPAPLDQPCCRALYDFDPENEGELGFKEGDIITLTNKIDDNWYEGMLQGNSGFFPINYVDILVPLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M494</accession>
    <name>A0A3B5M494_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WASH complex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Coiled-coil domain-containing protein 53</fullName>
        </alternativeName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="5">Component of the WASH complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC53 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M494"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018277"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018508.1">
        <property type="protein sequence ID" value="ENSXCOP00000018277.1"/>
        <property type="gene ID" value="ENSXCOG00000013765.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014084"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071203">
        <property type="term" value="C:WASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030041">
        <property type="term" value="P:actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000025">
        <property type="entry name" value="Putative WASH complex subunit CCDC53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019309">
        <property type="entry name" value="WASHC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13015">
        <property type="entry name" value="PROTEIN AD-016-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13015:SF0">
        <property type="entry name" value="WASH COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10152">
        <property type="entry name" value="CCDC53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="90"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="158"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="90"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="177"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00062975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="190" mass="20617" checksum="271F4D06340FBA39" modified="2018-12-05" version="1">MDEDGLPIVGSGVDLTKVPAIQQRRVVAHLNQFVVHTVRFLNRFSTVCEEKLSNISLRIQQIETTLCILEAKLSSIPGLEEVTVDGLNQQQSVQSNGPVSASQNQSDAAPAPEKAEVVPENVMTVAKDQRYARYLKMVQVGVPPMAIRNKMLMEGLDPNLLDTPDAPVPDGAENEDISATSSDSESSFSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5G1</accession>
    <name>A0A3B5M5G1_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">IF rod domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M5G1"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018677"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018912.1">
        <property type="protein sequence ID" value="ENSXCOP00000018677.1"/>
        <property type="gene ID" value="ENSXCOG00000009203.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165501"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006821">
        <property type="entry name" value="Intermed_filament_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050405">
        <property type="entry name" value="Intermediate_filament"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45652">
        <property type="entry name" value="GLIAL FIBRILLARY ACIDIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45652:SF13">
        <property type="entry name" value="NEUROFILAMENT LIGHT POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04732">
        <property type="entry name" value="Filament_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="7">
        <location>
            <begin position="102"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="441"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="120"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="331"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="448"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="499"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00061646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="516" mass="58442" checksum="51095833688CF8C3" modified="2018-12-05" version="1">MTADCPGCWPDSRFFFSSAAPEALWRMFGGGEAAVRGVVGVVSSPKTGASHRAYGSPSAVSSAAYRRTAAPGRVFSSIPDSAMDLTQSTAVTNELKIIRTNEKEQLQGLNDRFVSFIEKVHNLEQHNKVLEAEVTLLRQRHSEPSRLHELYEQEIRELRARVEELTHEKSQMHLDCVQMNETLERVREKLEEESRLREEAEGALKGYQKDVDDATLARLELEKKVESLLDEIAFLRKVHEEELQELQASLQATQVSVEMDMTKPDLAAALKDIRAQYENLSARNQVQAEEWYRSKFATVTEAAARNHDAIKHSKEELSEYRRQVQARTLEIEALRGHNEALERQIAEMEDRHNNEIGEMQDTIQQLEAALRSTKGEMSRHLREYQDLLNVKMALDIEIAAYRKLLEGEECRLSSVGSAMVQSGYPGLSYTSARAYALGAYRKPKPEEEAGEDEEKEEEEEAGEEEEGEEGEEQEEGEGEAEEEEEEEGEGEEEEEEEEEKPKGKEKESSTGKSSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5M6</accession>
    <name>A0A3B5M5M6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Integral membrane protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Negative regulator of amyloid-beta peptide production. May inhibit the processing of APP by blocking its access to alpha- and beta-secretase. Binding to the beta-secretase-cleaved APP C-terminal fragment is negligible, suggesting that ITM2C is a poor gamma-secretase cleavage inhibitor. May play a role in TNF-induced cell death and neuronal differentiation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with BACE1. Interacts with APP. Interacts with STMN2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Lysosome membrane</location>
            <topology evidence="14">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Membrane</location>
            <topology evidence="16">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the ITM2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M5M6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019463"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019703.1">
        <property type="protein sequence ID" value="ENSXCOP00000019463.1"/>
        <property type="gene ID" value="ENSXCOG00000014620.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183115"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001540">
        <property type="term" value="F:amyloid-beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042985">
        <property type="term" value="P:negative regulation of amyloid precursor protein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007084">
        <property type="entry name" value="BRICHOS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040145">
        <property type="entry name" value="ITM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10962:SF5">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10962">
        <property type="entry name" value="INTEGRAL TRANSMEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04089">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01039">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50869">
        <property type="entry name" value="BRICHOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 16" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="40"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="BRICHOS" evidence="17">
        <location>
            <begin position="122"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="253" mass="29645" checksum="B266CD5E6BF7AB2F" modified="2018-12-05" version="1">MVKITFQSVSAQKPEKDQDVDKITLPQGRLVPPMKPRKPFPVGLCCLAIGLVIFTLGLVLPSIYIYRFYFMSQIPEDSLFHCRVIYDDSVYAPLRGRQELEENVGIYLEENYEQISVPVPHFGGSDPADIVHDFQRGLTAYYDIALDKCYITELNTTAVMPPRSLWELLVNVKRGTYLPQTYIVQEEMVVTGRLRNMRQLGPFIHRLCFGKETYRLRRRNQRQRIERRETKKCHRIRHFENTFVVETVICDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M694</accession>
    <name>A0A3B5M694_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M694"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016916"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017135.1">
        <property type="protein sequence ID" value="ENSXCOP00000016916.1"/>
        <property type="gene ID" value="ENSXCOG00000012757.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165761"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="P:Golgi to secretory granule transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Spindly/BICDR_Dynein_Adapter"/>
    </dbReference>
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        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="283"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="68"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="283"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="309"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="319"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="340" mass="39656" checksum="49E50826544513B5" modified="2025-10-08" version="2">MELEEDFYFDYGAEEITDYQDPSELLAALKQKEEEVILAAQLGNALLLENRQLKEKSDKLHEQFTDRLEGLEQGRHELRLKLEGCQSQWESQVGELERDARELSAQVEQLTRALSEAEREKGRLQLEHGEQTQRLREELRTAMEVERAMSSELQALKEELQQKGGHNRQQDEELISAMKEQVLRLTQKEQELERRLESVSQENAELRASLASLQTRLAQHDQLDQQHSQQKNLMEAELVRSKNDMMCLNNQLLDAIQRKLELSQELEAWQPQDDIQIVINQQLKSQQQTEQPQKKPTSNGLPFFRRPSRTASTWTRQLSPSSSWSSDSNADKVQAPWREW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6M2</accession>
    <name>A0A3B5M6M2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Huntingtin-interacting protein 1-related protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Interacts with actin; homodimerization promotes actin binding. Interacts with CLTB. Interacts with HIP1. Interacts (via ENTH and I/LWEQ domains) with BCL2L10.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SLA2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6M2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017041"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017261.1">
        <property type="protein sequence ID" value="ENSXCOP00000017041.1"/>
        <property type="gene ID" value="ENSXCOG00000012858.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153594"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032051">
        <property type="term" value="F:clathrin light chain binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043325">
        <property type="term" value="F:phosphatidylinositol-3,4-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030837">
        <property type="term" value="P:negative regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051130">
        <property type="term" value="P:positive regulation of cellular component organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051050">
        <property type="term" value="P:positive regulation of transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030100">
        <property type="term" value="P:regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1410.10:FF:000002">
        <property type="entry name" value="Huntingtin interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1700:FF:000002">
        <property type="entry name" value="Huntingtin interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000012">
        <property type="entry name" value="Huntingtin interacting protein 1-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032422">
        <property type="entry name" value="HIP1_clath-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035964">
        <property type="entry name" value="I/LWEQ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002558">
        <property type="entry name" value="ILWEQ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030224">
        <property type="entry name" value="Sla2_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10407">
        <property type="entry name" value="HUNTINGTIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10407:SF14">
        <property type="entry name" value="HUNTINGTIN-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16515">
        <property type="entry name" value="HIP1_clath_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01608">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00307">
        <property type="entry name" value="ILWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109885">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50945">
        <property type="entry name" value="I_LWEQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="15">
        <location>
            <begin position="31"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="I/LWEQ" evidence="16">
        <location>
            <begin position="791"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="371"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="500"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1050" mass="117141" checksum="7685BA118D211DC5" modified="2018-12-05" version="1">MDRVKSSMQQVPNAIPKVIRRTGGANSLELERENFERGQAVSINKAINTQEVAVKEKHARNILTLCVCVCKGAHTFWAAVNRLPLSSNAVLCWKFCHVFHKLLRDGHPNVIKDSMRNKADLADMSRMWGHLSEGYGKLCSIYLKLLITKMEFHIKNPRFPGNLQMSNRQLDEAGENDVNNFFQLTVEMFDYLECELNLFLGVFNSLDMSRSVSVTAAGQCRLAPLIQVILDCSHLYDYTVKLLFKLHSCLPADTLQGHRDRFQEQFKKLKSLFYRSSNLQYFKRLIQIPQLPENPPNFLRASALSEHISPVVVIPAESSSPESEHVVETEDLVDTDVPSLPVKSSVKFDDLFGTSAAIDPFNFNSQNGMRKDDKDRLIEQLTREIQALKEELESFRLESGRLCQALRGRVNELEAELAEQSHLRLQAAGESEFLKAELDDLRRAREDTEKEQRSLTEIERKAQANEQRYTKLKEKYTELVQSHADLLRKNAEVTRQMTVARAAQDEVEAVRREMQEKVKSAQEAADRQEREQVKQLQELQRELVSTQTELDSLKTIMASSQQSSEVLGTQLVALESEKAELVQTLSKVEAELAVQGEELQRVQSSLATERESGVKTAEALQNQLNEKESREQALESQLVAARWSSLQGAVGEAKRIIQDSLCQIDDPAHISCTSSADYLASRCQASLDSMERLQSAREAFLADNTAVLELVRVVTQCGHLVGDTIVQGSATSHMVPVEQADALSESVKTCGSEALALLGQMKQQDSLAAADCGKLRAALEAILTTKLRPRGLELQQGELGDLVEQEMAATSAAVESAAARIEEMLNKSRAIDTGIKMEVNERILASCTELMQAIKELILSSKDLQRDIVESGRGAASMKEFYAKNSRWTEGLISASKAVGWGATVMVDAADMVVQGKGKFEELMVCSHEIAASTAQLVAASKVKADKDSANLHRLQQASRGVTQATAAVVASTKSGKSQIEDTETMDFSSMTLTQIKRQEMDAQVLVLELETRLQKERERLGELRKKHYELAGVAEGWGEEEEGEYYFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M7I0</accession>
    <name>A0A3B5M7I0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Xaa-Pro aminopeptidase 1</fullName>
            <ecNumber evidence="6">3.4.11.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Aminoacylproline aminopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Cytosolic aminopeptidase P</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Soluble aminopeptidase P</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">X-Pro aminopeptidase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">X-prolyl aminopeptidase 1, soluble</fullName>
        </alternativeName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Metalloaminopeptidase that catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro. Contributes to the degradation of bradykinin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.</text>
            <dbReference type="EC" id="3.4.11.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.9" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M7I0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019452"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019692.1">
        <property type="protein sequence ID" value="ENSXCOP00000019452.1"/>
        <property type="gene ID" value="ENSXCOG00000014609.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157716"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01085">
        <property type="entry name" value="APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000001">
        <property type="entry name" value="Putative xaa-Pro aminopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000004">
        <property type="entry name" value="xaa-Pro aminopeptidase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000004">
        <property type="entry name" value="xaa-Pro aminopeptidase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033740">
        <property type="entry name" value="Pept_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032416">
        <property type="entry name" value="Peptidase_M24_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050422">
        <property type="entry name" value="X-Pro_aminopeptidase_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763:SF6">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16189">
        <property type="entry name" value="Creatinase_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16188">
        <property type="entry name" value="Peptidase_M24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase N-terminal" evidence="23">
        <location>
            <begin position="49"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24" evidence="22">
        <location>
            <begin position="329"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24 C-terminal" evidence="24">
        <location>
            <begin position="550"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00054066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00071973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00078157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00079909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00079919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00081885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF16188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="613" mass="68932" checksum="E610BEEC7E68BBAD" modified="2018-12-05" version="1">MSPKITGELLRLLRQAMRNCKYFSEPIHAYIVPSGDAHQSEYIAPCDCRREFICGFNGSAGTAIITEQHAAMWTDGRYFLQASQQMDNNWALMKMGLKETPSQEDWLINVLPENSKVGVDPWIIAADQWKNMSKALTSAGHSLVAVQDNLIDVVWTDRPDRPSTQLHTLGLEYTGISWQDKITMLRGKMTERKISWFVATALDEIAWLFNLRGADINYNPVFFAYAIVGMNSIRLFVDLKRLSDTVVRDHLQLDSPRRPELSVQTFPYESVYTELQAICAALGPKDKVWICDKASCALMQVVPKAHRTPIPYTPLCLSKAVKNATEIKGMKMAHIKDAVALCELFAWLEKEDVLNEFLSVFLSSQQKDFVGLSFPTISSVGPNGAIIHYRPLPETNRTLTVNEVYLIDSGAQYIDGTTDVTRTVHFGTPSAFEKECFTYVLKGHIAVSAAIFPNGTKGHLLDSFARAALWESGLDYLHGTGHGVGCFLNVHEGPCGISYKTFADEPLEAGMIVSDEPGYYEDGSFGIRIENVVLVVPAKPKYNYRNRGSLTFEPLTLVPIQVKMMNTELLTQKEKDWVNEYHRRCREVVGAELERQGRMEALQWLVRETQPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAD4</accession>
    <name>A0A3B5MAD4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAD4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021213"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021473.1">
        <property type="protein sequence ID" value="ENSXCOP00000021213.1"/>
        <property type="gene ID" value="ENSXCOG00000015870.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160104"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000068">
        <property type="entry name" value="transmembrane protease serine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000027">
        <property type="entry name" value="Transmembrane serine protease 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF20">
        <property type="entry name" value="LOW QUALITY PROTEIN: TRANSMEMBRANE PROTEASE SERINE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="CUB" evidence="6">
        <location>
            <begin position="243"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="7">
        <location>
            <begin position="476"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="357"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="373"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="409"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="430"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="450"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="662" mass="74656" checksum="3A2F8116EE56958E" modified="2018-12-05" version="1">MLFSKTFGTSFLFCLHNESWRFDLMSHKYEQFKMCQQLIVHIYGLNSNVCCSLSVTEYSVWTLEPRVQQQYTAYLTIVNRNFSADLACHSNPVFKTEAKYVENTVSRKHAFFSIRVHTDTYCTVLTSSSSDCYRYQKVVSSSPVALRGPETHRPSCLWHLQTDPGSQLELHMEWLLPECRDRLVVYDSITPSDHHLITSVYGCSRHEDVVRVLSSGEWMTVVWKKGLYNYKDPFSLSAQAWPQQGKTEGVQGILQTPFFPSFYPPNTNCTWTFIVTLGLALEFEGYELSRASYNQACTQGQWVIQNRRLCGTRGLQPYCERLSLLTHSTTVVMTSEVSLTGPGLQVHYSIFNLSDPCPGQFLCTVSGLCVPLCDGIKDCSNGLDERNCVCVAQYKCPEDSQCVDYYKVCDQHPDCPEASDEMNCTDGVECTDMTYVCADGTCLKKPNPECDSVTDCPDASDENRCECGLRQFSTRIVGGSDASEGEWPWQTSLQVRGTHICGGALISSQWVASAAHCFYDDSVYSPSVWTVYLGKLLLNRSSSIEEVARVQQIHLHQYYDSETHDYDLALLKLDRPAYALLTGHARPACLPPATHQLEPGLLCWVTGWGAQQEGGELANRSEISGRKRSGQGIETKPKYYSIWKDHVTEFKVCQSTLEENVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MBV2</accession>
    <name>A0A3B5MBV2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Discs, large homolog 3 (Drosophila)</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MBV2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021082"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021341.1">
        <property type="protein sequence ID" value="ENSXCOP00000021082.1"/>
        <property type="gene ID" value="ENSXCOG00000015760.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159565"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035255">
        <property type="term" value="F:ionotropic glutamate receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097120">
        <property type="term" value="P:receptor localization to synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099072">
        <property type="term" value="P:regulation of postsynaptic membrane neurotransmitter receptor levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06723">
        <property type="entry name" value="PDZ1_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06724">
        <property type="entry name" value="PDZ2_Dlg1-2-4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12029">
        <property type="entry name" value="SH3_DLG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000008">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000001">
        <property type="entry name" value="Disks large homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000091">
        <property type="entry name" value="disks large homolog 1 isoform X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000027">
        <property type="entry name" value="Disks large homolog 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000002">
        <property type="entry name" value="Disks large homolog 4 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019583">
        <property type="entry name" value="DLG1-4_PDZ_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016313">
        <property type="entry name" value="DLG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019590">
        <property type="entry name" value="DLG1_PEST_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035763">
        <property type="entry name" value="DLG3_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050614">
        <property type="entry name" value="Synaptic_Scaffolding_LAP-MAGUK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119">
        <property type="entry name" value="DISCS LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119:SF28">
        <property type="entry name" value="DISKS LARGE HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10608">
        <property type="entry name" value="MAGUK_N_PEST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10600">
        <property type="entry name" value="PDZ_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001741">
        <property type="entry name" value="MAGUK_DLGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01277">
        <property type="entry name" value="MAGUK_N_PEST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PDZ" evidence="9">
        <location>
            <begin position="150"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="9">
        <location>
            <begin position="245"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="9">
        <location>
            <begin position="408"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="509"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="8">
        <location>
            <begin position="655"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="43"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="85"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="855" mass="94428" checksum="E89724003330310C" modified="2018-12-05" version="1">MHKHHHCCKCPECYEVTRMAALRRMDPPAYGGDWQAEPYGYPPGYGGGQTLPPPASGGGGGGGGGTLGRSKGKIMSGGGPGGPNPKGQSKCGPKVNGNNSSGQGGWWPECTCTNREWYDQANPPPIIVNADSLDAGPYVNGSDGMFKYEEIILERGNSGLGFSIAGGIDNPHIPDDPGIFITKIIPGGAAAMDGRLGVNDCVLRVNEVDVSEVVHSRAVEALKEAGPVVRLLVRRRQAPPETILEVNLLKGPKGLGFSIAGGIGNQHIPGDNSIYITKIIEGGAAQKDGRLQTGDRLLAVNNIVLQDVRHEEAVAALKNTSDMVYLKVAKPGPVHLNDMYAPPDYSSSTYLNRPVAFPTMVDNHVSHSYMGAMEPKPVYPPPQVTPSRYSPVPRHMLGEEDFTREPRKVVLHKGSTGLGFNIVGGEDGEGIFVSFILAGGPADLSGELRRGDRILSLYYCTHGVAACLLKTPFPIFEYSRFESKIHDLREQMMNSSMSSGSGSLRTSEKRSLYVRALFDYDRARDSCLPSQGLSFSYGDILHVINASDDEWWQARLVTPHGESEQIGVIPSKKRVEKKERARLKTVKFHARTGMIESNRQVKVKRKKSFNLSRKFPFYKSKENIVQELVETEQCLTSNTSDSESSSKGQEDTILSYEPVIRQESEWQLCALRKCLVSYHRVNSLFFFPTILFASADTTRPRRETEIDGQDYHFVGSREQMEKDIQDNKFIEAGQFNENLYGTSILSVRTVAERGKHCILDVSGNAIKRLQQAQLYPIAIFIKPKSVEALMEMNKRQTYEQANKVFDKAVKLEQDFGEYFTAIVQGDSLEEIYNKIKLIIEEQSGPYIWIPSPEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MC10</accession>
    <name>A0A3B5MC10_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM163 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027857331.1">
        <property type="nucleotide sequence ID" value="XM_028001530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MC10"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018806"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019042.1">
        <property type="protein sequence ID" value="ENSXCOP00000018806.1"/>
        <property type="gene ID" value="ENSXCOG00000014160.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114134750"/>
    <dbReference type="KEGG" id="xco:114134750"/>
    <dbReference type="GeneTree" id="ENSGT00940000159228"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029379">
        <property type="entry name" value="FAM163"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040281">
        <property type="entry name" value="FAM163A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31914">
        <property type="entry name" value="PROTEIN FAM163A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31914:SF2">
        <property type="entry name" value="PROTEIN FAM163A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15069">
        <property type="entry name" value="FAM163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="154" mass="16334" checksum="EFD31A9FF0A43BA8" modified="2018-12-05" version="1">MSAGTIVITGGILAGVILLCIVAVLCYCRLQYYCCKKNESEADVGSVAGADPLSHFPCNACNALAMDGTDITPVSLDQLDSGSHHSPCPSCSLYPVRSGLTDDMRNGGERLGFYYENPSVSLPLPVNHPTSSPLSYYNPTDAFPAPPRPYSTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MCE0</accession>
    <name>A0A3B5MCE0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MCE0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000017364"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017585.1">
        <property type="protein sequence ID" value="ENSXCOP00000017364.1"/>
        <property type="gene ID" value="ENSXCOG00000013085.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050149">
        <property type="entry name" value="Collagen_superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24023:SF1082">
        <property type="entry name" value="COLLAGEN TRIPLE HELIX REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="24"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="111"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="43"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="253" mass="27408" checksum="F7AFB74030E4AB2B" modified="2018-12-05" version="1">MVVRNHLLCCHGNSCDSLVQGLQGSKGHRGLPGPSGARGATGPPGPPGRSRPGRPGPMGPKGANGYMGDRGAPGPRGQFVRRGLGRNRAQSGPEPGPERRLTALLVSGEKGSKGNIWNIPGEPGPKGSRGQPGETGPEGKRRIQTLVLVLVLFPASHRKKHKRILKTNINKDLFIRSKYFKRFHRQCYNSVPVLVRSWSGRGPVLIRFCSSMLSLFSFGPSFKYYICMDVKLLLSLKITIFLNCSAKLCSLAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MDQ3</accession>
    <name>A0A3B5MDQ3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MDQ3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021762"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022026.1">
        <property type="protein sequence ID" value="ENSXCOP00000021762.1"/>
        <property type="gene ID" value="ENSXCOG00000016263.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064469"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12752:SF2">
        <property type="entry name" value="PDZ AND PLECKSTRIN HOMOLOGY DOMAINS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12752">
        <property type="entry name" value="PHOSPHOINOSITOL 3-PHOSPHATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="15"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="255" mass="29428" checksum="1C38897AEB89C0E8" modified="2025-10-08" version="2">MTIGSDIARGPNTPRPPCRGYLHKRTQSGLIKGWRKRWFVLTHDCCLYYFKNKRDEGKRQPLSSVKLEGAEVGPDDSLGKPFVFKCRPQFGSRVYSLCATSNQEMKWCEAMERAVHPITQQNHVWVDVTRHNSNVPPLAVKNPDCLGLLNKLDRSKDTWVQHYCILKDACLYLYSGIRSGIYLQGYVVREQANGSKKSTIELKPLSDEFKTFYFCADNSAENKWILAIKASIKKWRPFQQVLQDYMSLPPEETRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MER9</accession>
    <name>A0A3B5MER9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sulfide:quinone oxidoreductase, mitochondrial</fullName>
            <ecNumber evidence="14">1.8.5.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Sulfide quinone oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the oxidation of hydrogen sulfide with the help of a quinone, such as ubiquinone-10, giving rise to thiosulfate and ultimately to sulfane (molecular sulfur) atoms. Requires an additional electron acceptor; can use sulfite, sulfide or cyanide (in vitro). It is believed the in vivo electron acceptor is glutathione.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a quinone + hydrogen sulfide + glutathione + H(+) = S-sulfanylglutathione + a quinol</text>
            <dbReference type="Rhea" id="RHEA:55156"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58905"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.8.5.8"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:55157"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ubiquinone-10 + hydrogen sulfide + glutathione + H(+) = S-sulfanylglutathione + ubiquinol-10</text>
            <dbReference type="Rhea" id="RHEA:62608"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:46245"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58905"/>
            <dbReference type="ChEBI" id="CHEBI:64183"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:62609"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ubiquinone-10 + hydrogen sulfide + sulfite + 2 H(+) = ubiquinol-10 + thiosulfate</text>
            <dbReference type="Rhea" id="RHEA:38359"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:33542"/>
            <dbReference type="ChEBI" id="CHEBI:46245"/>
            <dbReference type="ChEBI" id="CHEBI:64183"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:38360"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the SQRD family.</text>
    </comment>
    <dbReference type="EC" id="1.8.5.8" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MER9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022060"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022328.1">
        <property type="protein sequence ID" value="ENSXCOP00000022060.1"/>
        <property type="gene ID" value="ENSXCOG00000016484.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000019406"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106436">
        <property type="term" value="F:glutathione-dependent sulfide quinone oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070224">
        <property type="term" value="F:sulfide:quinone oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070221">
        <property type="term" value="P:sulfide oxidation, using sulfide:quinone oxidoreductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000034">
        <property type="entry name" value="sulfide:quinone oxidoreductase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015904">
        <property type="entry name" value="Sulphide_quinone_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10632">
        <property type="entry name" value="SULFIDE:QUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10632:SF2">
        <property type="entry name" value="SULFIDE:QUINONE OXIDOREDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0874">Quinone</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="17">
        <location>
            <begin position="43"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00051038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00059167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00082958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="436" mass="48835" checksum="F819F9258BDE2907" modified="2018-12-05" version="1">MICPQRTRISMASSWRRLRLCHSTGVAISHLHTSSRASAKQHYKMLVLGGGSGGICMSARMKRMFGADNVAVVEPSEVHYYQPIWTLVGAGAKTVASSARPTASVMPSGVKWVKSKVQEIHPDKNSVLVDDGTEISYEYLIVALGLQLHYEKIKGLPEAFEHPKIGSNYSVQTVEKTWKALQNFEEGNAVFTFPNTPVKCAGAPQKIMYLSDAYLRKTGKRAKANVIYNTSLPVLFGIKKYADTLWDIVKTRDLQVNLRHNLIEVRPDKQEAVFENLDKPGETKVFEYEMLHVTPPMGPSLVVKGSPLADENGWLDLNKETLQHNKYPNVFGIGDCTNLPTAKTGAAVAAQSAILNRTISKLLKNQKPDKKYDGYTSCPLVTSYNTVVLAEFDYNGQPLETFPINQAKERRLMYYMKADVMPHLYWHGLLRYAYDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MFR3</accession>
    <name>A0A3B5MFR3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Follistatin-like 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MFR3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019961"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020208.1">
        <property type="protein sequence ID" value="ENSXCOP00000019961.1"/>
        <property type="gene ID" value="ENSXCOG00000014990.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156495"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030510">
        <property type="term" value="P:regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00104">
        <property type="entry name" value="KAZAL_FS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:002358">
        <property type="entry name" value="Follistatin like 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.30:FF:000007">
        <property type="entry name" value="follistatin-related protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050653">
        <property type="entry name" value="Prot_Inhib_GrowthFact_Antg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913">
        <property type="entry name" value="FOLLISTATIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10913:SF44">
        <property type="entry name" value="FOLLISTATIN-RELATED PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00280">
        <property type="entry name" value="KAZAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Kazal-like" evidence="7">
        <location>
            <begin position="66"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="153"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="228"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="327" mass="37357" checksum="4B7215C160108B5A" modified="2018-12-05" version="1">MQPKYSIIKLFRCINTSNINGFIFCHCVFSLGFMGHSSYPGPCVHKYCGLGRHCVINHETDQGECRCLDHCKPHYKPVCGSDGKLYQNHCELHRASCLRGHRITIMHSEECFYKDDDCRLNDYRRLKTKILDLHDKRYLGAAGHGAHKDSMAAKKQLVDLMFKRFDVDRNGLIDASELSQVIKHEGLSKDVSECTLFDLLKYNDINNDEHLTKEEFYTAFDVYLLNLPDEQKVSVTTVTVGQSAVMTCAITGERRPPILWKRNNQYLNPLNLEDLNDFGDDGSLYIIKVTTTHMGNYTCHADGYDKLFQTHILHVNGKKVISTVPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MGL9</accession>
    <name>A0A3B5MGL9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MGL9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022887"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023162.1">
        <property type="protein sequence ID" value="ENSXCOP00000022887.1"/>
        <property type="gene ID" value="ENSXCOG00000017096.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159069"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08374">
        <property type="entry name" value="C2F_Ferlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037725">
        <property type="entry name" value="C2F_Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037721">
        <property type="entry name" value="Ferlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546">
        <property type="entry name" value="FER-1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12546:SF37">
        <property type="entry name" value="FER-1-LIKE 6 (C. ELEGANS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="C2" evidence="6">
        <location>
            <begin position="43"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="175" mass="20139" checksum="230A880B649DFB32" modified="2018-12-05" version="1">IPAVGCKLVPEHIETRILYNKARPGMDQGQVQMWVDMFPMDLPHPGPPVDISPRKPKGYELRIIIWNTEDVILEDSNFLTGQQSSDIYIKGWLKGLEDDRQETDVHYNSLTGEGNFNWRFVFPFSYLPAEKVVVVKKRDHIFSLDKTEQKLPAILIMQVWDFETLSSDDFLGGCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MH99</accession>
    <name>A0A3B5MH99_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ion transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MH99"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023688"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023971.1">
        <property type="protein sequence ID" value="ENSXCOP00000023688.1"/>
        <property type="gene ID" value="ENSXCOG00000017693.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159763"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097682">
        <property type="term" value="F:intracellularly phosphatidylinositol-3,5-bisphosphate-gated monatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015280">
        <property type="term" value="F:ligand-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022832">
        <property type="term" value="F:voltage-gated channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075509">
        <property type="term" value="P:endocytosis involved in viral entry into host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028798">
        <property type="entry name" value="TPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46768">
        <property type="entry name" value="TWO PORE CALCIUM CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46768:SF1">
        <property type="entry name" value="TWO PORE CHANNEL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="6">
        <location>
            <begin position="25"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="322" mass="37459" checksum="45DC635F7BA02581" modified="2018-12-05" version="1">VVFSHYYLTVLGNLVALANVLCICVSSVINLCFILFYLSEMSVKIFAFGWRGYLSYRSNIFDGFLTVLLLILQIAIFITYRLPYSQEPSSRGVMSLWEMVRLVNMLIVFRFLRIIPDIKLMALVASTLLDLVKNLRAFAGILVVVYYVFAVFGIWLFEGAIKPPPQMSVFSMDNTTSNFSTACGTYEQLGYWPNNFDDFAAAIILLYDVMIVNNWQAFMEAYSRYTSEWSKVYFVCWWLTSSVMWVNLFVALILENFIYKWDRSHSISVRSSLIPLACFLREQIQEPTEEELACQLHQHPHLHLEWRHEHQNIRPSLPSLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MHM0</accession>
    <name>A0A3B5MHM0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MHM0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023393.1">
        <property type="protein sequence ID" value="ENSXCOP00000023115.1"/>
        <property type="gene ID" value="ENSXCOG00000017271.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003839"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032016">
        <property type="entry name" value="MKRN2OS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053921">
        <property type="entry name" value="MKRN2OS-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053922">
        <property type="entry name" value="MKRN2OS-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33963">
        <property type="entry name" value="MKRN2 OPPOSITE STRAND PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33963:SF2">
        <property type="entry name" value="MKRN2 OPPOSITE STRAND PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16044">
        <property type="entry name" value="DUF4796_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22795">
        <property type="entry name" value="DUF4796_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MKRN2 opposite strand protein-like N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="MKRN2 opposite strand protein-like C-terminal" evidence="1">
        <location>
            <begin position="40"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="217" mass="24905" checksum="2FE2DD861F94F822" modified="2018-12-05" version="1">MEQSVIHLSHCQKEIFCFAVPEVCPSCGEELKGRRLQEAPVSLPSPFTDGHKTSCCLLVAPKTFSRQDFTGTSDLHTGISNTKGVVYNYTQDGVRRDQSGWSRCISIPLVRPDMFHLLAQWDQYLESFSEGPAWDPVFHEEDHNCFSFCLQFLNGILAMEGRGALSRDDFTRCFILPRMRRVSKYTTLIRHLQDHQYYLTDRQETLGDRQEESVTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIS6</accession>
    <name>A0A3B5MIS6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoinositide 3-kinase regulatory subunit 5</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIS6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021021"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021279.1">
        <property type="protein sequence ID" value="ENSXCOP00000021021.1"/>
        <property type="gene ID" value="ENSXCOG00000015727.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063753"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005944">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class IB"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046935">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019522">
        <property type="entry name" value="PIK3R5/6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15593">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15593:SF2">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10486">
        <property type="entry name" value="PI3K_1B_p101"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="234"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="239"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="266"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="306"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="320"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="785" mass="87768" checksum="800D9A1ADD4771CE" modified="2018-12-05" version="1">MVLQTMEQSSCTEDRIQHSLERCLCHLGVDSPDKKLWNAGLCISRWCLEELVKRHPHNFLILLQKILQKTKEVLEECQYELVVPLTLLFSAFLVVESELLKHLQSYWILFTHYCLFIAGISFQRLVRTEQAISPDLNCSKTLVVLLVSPDADTPQEVQLTSEQLSTMHYSSRNTITTLILHSFQAAFGTKLDLRVLHAALQYCFPNSRSCLLVHPDVLKDLLLVDSDLDSPPECFPKIDEDDTFDTSIDEEDEQDSSSKPDDPFQSHRISTASSSSRDSTFSGYSLSSSWSVPSASSGVESDFSEDTAHEDTKEGQGRQLKVRKKPKKKSKSLLGLERFSLLFKTPHSPSTSRRVRSMGYNGDITKDYLMMGAQLKHSGTPMRHIRHLNVSTAPVDPSAPQRHICVRRRPILSCSEANEAEVLTVVKVVAFGGDREAGRLARAYSDLQQKERKCPQLTRTCKLQFYFVPTKRKSVSPGAGHMPAEGQVLQTLRKRFDAFSSEKSLRQVEDSTTDIAQMLGMMDPWYERNVFSLLSLSSEVLCEDDVSGGTGATEHRLPLLADLVLYYCRHADQPVLVQLYQAELTLEGGEKRKEVFIHSLELGHTAGARAVKAMGCSSHPQCGLQQGIVPLRTLPALQTKGSISGRSHWMHKEAVCTSINLQKTCRESKQLDSKEGLQLTMTEVVKKQSSKSKRGYNQILISEVKVDKMQVTAQGDGSTFAVCLDQDKKTFIQSVKRCEVSLCSKPGSSSDWRSYKTLPGQIQPLHPTFCSLLCLPVTAFSAADP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MIY4</accession>
    <name>A0A3B5MIY4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP synthase subunit d, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATPase d subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027873759.1">
        <property type="nucleotide sequence ID" value="XM_028017958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MIY4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019519"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019759.1">
        <property type="protein sequence ID" value="ENSXCOP00000019519.1"/>
        <property type="gene ID" value="ENSXCOG00000014666.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114144781"/>
    <dbReference type="GeneTree" id="ENSGT00390000003582"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008689">
        <property type="entry name" value="ATP_synth_F0_dsu_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036228">
        <property type="entry name" value="ATP_synth_F0_dsu_sf_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12700">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT D, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05873">
        <property type="entry name" value="Mt_ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005514">
        <property type="entry name" value="ATPase_F0_D_mt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161065">
        <property type="entry name" value="ATP synthase D chain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5 10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="161" mass="18141" checksum="8E94FEEB208C159E" modified="2018-12-05" version="1">MAGRRAALKAIDWLAFAERVPPNQRGMFNALKTRTDAISAKLASLPETPAAIDWSYYRSAVAKAGMVDEFEKKFKALQIPEPVDTQTSNINAQEAEANKSAVAYIEASKARVATYEQELEKFKNMIPFDQMTIDDLNEAFPETKLDKVKYPYWPHKPIANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJ87</accession>
    <name>A0A3B5MJ87_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Relaxin-3 receptor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Relaxin family peptide receptor 3</fullName>
        </alternativeName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Receptor for RNL3/relaxin-3. Binding of the ligand inhibit cAMP accumulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJ87"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024398"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024692.1">
        <property type="protein sequence ID" value="ENSXCOP00000024398.1"/>
        <property type="gene ID" value="ENSXCOG00000018220.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234518"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000216">
        <property type="entry name" value="Relaxin family peptide receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF935">
        <property type="entry name" value="RELAXIN FAMILY PEPTIDE RECEPTOR 3.3A1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00526">
        <property type="entry name" value="FMETLEUPHER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="149"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="48"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="375" mass="42324" checksum="37A2ADD50F478284" modified="2018-12-05" version="1">RRSLSSSAEPPLRTFTFDDIDVSADGSPILRIFISVVYSVVCAVGLVGNLLVFFLMRVRQGRKRSTINVFITNLAVTDFQFVLTLPFWAVDTALDFSWPFGDAMCKIVLSVTVMNMYASVFFLTAMSVTRYLSVASALKKRSYRRSRCVKWVCAVLWLAATAATAPTAVFSTVTVVAGEKLCLLRFPEGHDWLALYHIQKILIAFVIPMFIVCVNYMMLLRFVRRKSMDGSNPKRRTRVTKSVAIVVLSFFCCWMPNHAITFWGVLVKFNAANWDTSYYTVHTYVFPVTVCLAHTNSCLNPILYCLMRPEIRKMLSGLFWRVPSPTQSKACATRSVTHIETQGVVPLQIIDNTEYTLSIIDRKGSSSSKSIPYTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJT4</accession>
    <name>A0A3B5MJT4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Galectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LGALS2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJT4"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021647.1">
        <property type="protein sequence ID" value="ENSXCOP00000021386.1"/>
        <property type="gene ID" value="ENSXCOG00000016010.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155025"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00070">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000021">
        <property type="entry name" value="Galectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044156">
        <property type="entry name" value="Galectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001079">
        <property type="entry name" value="Galectin_CRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11346:SF104">
        <property type="entry name" value="GALECTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00337">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00908">
        <property type="entry name" value="Gal-bind_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00276">
        <property type="entry name" value="GLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51304">
        <property type="entry name" value="GALECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Galectin" evidence="3">
        <location>
            <begin position="4"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU102079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="145" mass="16492" checksum="C0DF40B510E754A6" modified="2018-12-05" version="1">FPQQLELKNVTLRPGDKLKLKGEILHDAEKFRIDLGVSSEDLALHFNPRFQDDADGAVLVYNSKTAGSWGSEGREMHNPLQRGNDVKVRKSGSRTKWFQDVLIVELPDGQKLKFPNRTNMDVITYVQVAGDFKLKSFKIYKSVKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MJX4</accession>
    <name>A0A3B5MJX4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Copine Vb</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the copine family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MJX4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024649"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024947.1">
        <property type="protein sequence ID" value="ENSXCOP00000024649.1"/>
        <property type="gene ID" value="ENSXCOG00000018414.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156194"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01459">
        <property type="entry name" value="vWA_copine_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000013">
        <property type="entry name" value="copine-9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF51">
        <property type="entry name" value="COPINE-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="6">
        <location>
            <begin position="93"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="7">
        <location>
            <begin position="275"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="511"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="531" mass="59681" checksum="E39FE8FD6B3DA252" modified="2018-12-05" version="1">YTTECGKHFSCSTFLDQVLYKISAIIFCFGRTEVIDNTLNPDFVRKYILDYFFEEKQNLRFDFYDVDSKSPDLSKHDFLGQMFCTLGEIVGSPASRLEKPLGGIPGKKCGTIILTAEELSNCRDSATMQFCANKLDKKDFFGKSDPFMVFYRSNEDGTFTICHKTEVVKNTLNPVWQPFTIPVRALCNGDYDRTIKVEVYDWDRDGSHDFIGDFTTSYRELARGQSQFNVYEVINTKKKMKKKKYVNSGTVTLLSFSVESEFTFLDYIKGGTQINFTVAIDFTASNGNPSQSTSLHYMNPYQLNAYAMALKAVGEIIQDYDSDKMFPALGFGAKLPPDGRVSHEFPLNGNIENPYCNGIEGILEAYHQSLKTVQLYGPTNFAPIVNHVARYAAAVQDGSQYFVLLIITDGVISDMAQTKEAIVNAAKLPMSIIIVGVGQAEFDAMVELDGDDIRISSRGKLAERDIVQFVPFRDYMDRTGNHVLSMARLAKDVLAEIPDQFISYMKSRGIKPNPAPPPYTPPGHTHHHTQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MKE4</accession>
    <name>A0A3B5MKE4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rab GTPase-binding effector protein 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Plays a role in membrane trafficking and in homotypic early endosome fusion. Participates in arteriogenesis by regulating vascular endothelial growth factor receptor 2/VEGFR2 cell surface expression and endosomal trafficking. By interacting with SDCCAG8, localizes to centrosomes and plays a critical role in ciliogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Early endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the rabaptin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MKE4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024222"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024513.1">
        <property type="protein sequence ID" value="ENSXCOP00000024222.1"/>
        <property type="gene ID" value="ENSXCOG00000018091.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063743"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.730">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003914">
        <property type="entry name" value="Rabaptin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018514">
        <property type="entry name" value="Rabaptin_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015390">
        <property type="entry name" value="Rabaptin_Rab5-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31179">
        <property type="entry name" value="RAB GTPASE-BINDING EFFECTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31179:SF6">
        <property type="entry name" value="RAB GTPASE-BINDING EFFECTOR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09311">
        <property type="entry name" value="Rab5-bind"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03528">
        <property type="entry name" value="Rabaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103652">
        <property type="entry name" value="G protein-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="13 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rabaptin coiled-coil" evidence="19">
        <location>
            <begin position="15"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="Rabaptin GTPase-Rab5 binding" evidence="20">
        <location>
            <begin position="202"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Rabaptin GTPase-Rab5 binding" evidence="20">
        <location>
            <begin position="303"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="17"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="191"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="305"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF03528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF09311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="451" mass="50386" checksum="2503B7D1EA6AFB8D" modified="2018-12-05" version="1">TKPSPSYKMTEADFMNLASLQAQLAEYRAQAEHWQGVATICELSKQEELAELQKQCDEEIQSLQEALRETAAQYEARIAVLQSQPVDWRRTSGHNAVKDEAASLADQTRSQPAESEAAAEGESSALSAEGYFSLQNCDSASLSSFSLDTPSLPRKLLAQDDTDSLVSTGTLVPEAIYLPPAGHRLVTHIDWDTMNAQLSELRGEVSRLQAEKEELERELDTHTHQTHKQVSLLQSQVQASETLLQDLQKSFSQSQNAVQSRLAELSLSQRKMCCELSRLRGEEVDESLLDPMSSLSSAQHGAHCEERLRIEIVNLREQLDSRTEENVQLSTLRTETEKIQAQKDQQQAELLSCRSELDALRVALTHLQSSNKTLTHDKAVLHQQCLELRSQVISLRSQVDTSQTVQRDFVELSQSLQVKLELIRQAESLEQVKEILGESVREAESQPADAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5ML53</accession>
    <name>A0A3B5ML53_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5ML53"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024310"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024603.1">
        <property type="protein sequence ID" value="ENSXCOP00000024310.1"/>
        <property type="gene ID" value="ENSXCOG00000018158.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01130000278263"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008528">
        <property type="term" value="F:G protein-coupled peptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24230:SF163">
        <property type="entry name" value="CORAZONIN RECEPTOR, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24230">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="156"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="56"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="189" mass="20569" checksum="600B8CBD198277EB" modified="2018-12-05" version="1">MRDPVADPHVNVTSNCSLAASNLTAVADVPQLPTFTTAAKVRVIITFVLCGASAFCNLAVLWAAHRDGKRKSHVRVLIVNLTVADLLVTFVVMPVDAAWNITVQWLAGDFACRLLMFLKLLAMYSCAFVTVVISLDRQAAILNPLAINKARMRNRIMLAVAWGVSVALSIPQVRNTLQEPTVSETVSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MLA2</accession>
    <name>A0A3B5MLA2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptogyrin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MLA2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000024355"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024648.1">
        <property type="protein sequence ID" value="ENSXCOP00000024355.1"/>
        <property type="gene ID" value="ENSXCOG00000018188.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182935"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016579">
        <property type="entry name" value="Synaptogyrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10838">
        <property type="entry name" value="SYNAPTOGYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10838:SF7">
        <property type="entry name" value="SYNAPTOGYRIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="8">
        <location>
            <begin position="21"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000024355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="119" mass="13233" checksum="6E008ADCB9E0F055" modified="2018-12-05" version="1">MDGFQAYGAGKAGGAFDPLTFIRQPQTVIRILCWLFSIVILGCVANEGYVNRPDEVEEYCIFNRNQNACNYAIAMGTLGFLCSAGFLALDVYFPQISGVKDRKKAVMLDIGVWKMLVFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MMT9</accession>
    <name>A0A3B5MMT9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 17">Alkylglycerone-phosphate synthase</fullName>
            <shortName evidence="17">Alkyl-DHAP synthase</shortName>
            <ecNumber evidence="7 17">2.5.1.26</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Catalyzes the exchange of an acyl for a long-chain alkyl group and the formation of the ether bond in the biosynthesis of ether phospholipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a long chain fatty alcohol + a 1-acylglycerone 3-phosphate = a 1-O-alkylglycerone 3-phosphate + a long-chain fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36171"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17135"/>
            <dbReference type="ChEBI" id="CHEBI:57534"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:73315"/>
            <dbReference type="EC" id="2.5.1.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16 17">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Glycerolipid metabolism; ether lipid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 17">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 17">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.26" evidence="7 17"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MMT9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025739"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026049.1">
        <property type="protein sequence ID" value="ENSXCOP00000025739.1"/>
        <property type="gene ID" value="ENSXCOG00000019237.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156112"/>
    <dbReference type="UniPathway" id="UPA00781"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008609">
        <property type="term" value="F:alkylglycerone-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008611">
        <property type="term" value="P:ether lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.330:FF:000001">
        <property type="entry name" value="Alkylglycerone-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.10:FF:000003">
        <property type="entry name" value="Alkylglycerone-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025650">
        <property type="entry name" value="Alkyl-DHAP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46568">
        <property type="entry name" value="ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46568:SF1">
        <property type="entry name" value="ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16 17" id="KW-0274">FAD</keyword>
    <keyword evidence="9 17" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 17" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 17" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 17" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="18">
        <location>
            <begin position="110"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="14">
        <location>
            <position position="450"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="142"/>
            <end position="148"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="224"/>
            <end position="227"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR625650-1"/>
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    </evidence>
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            <dbReference type="PIRSR" id="PIRSR625650-2"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR625650-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="494" mass="55407" checksum="80F557241EC29171" modified="2018-12-05" version="1">MKWNGWGYSDSRFLFNKKGQAEFTGKRYRLSGMIIPGLKDWMEGTFGANLQHKTQPVLNTSAVQPPMLNEAFVEELKSTGIPFSHEADDRVFRAHGHCLHEIFALRDGKIGRVPDMVVWPNCHNDVVKIVELACKHNVCLIPYGGGTSVSSALECPPEETRSIVSLDTSQMNRILWIDEKNLTAHVESGIVGQDLERLLNESGYCTGHEPDSMEFSSLGGWVATRASGMKKNIYGNIEDLVVHIKMVTPRGVIEKSCQGPRMSTGPDIHHFILGSEGLLTAIIKILYLRDHEKYSESLIDMQKLYHGLSSIFTSFLDGLKKFYITKFKGFDPNRLCVATLLFEGAREKVLQHEKQVYDIAAKFGGLAAGEDNGQRGYMLTFVIAYLRDLGMDYYVIGESFETSVPWDRVLDICRNVKARIIRECKDKGVQFPPLSTCRVTQTYDAGACVYFYFAFNYRGLSDPVHVYEQVEHAAREEILANGGSLSHHHGGTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MNQ2</accession>
    <name>A0A3B5MNQ2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">E3 ubiquitin-protein ligase RNF123/RKP TPR repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MNQ2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022791"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023067.1">
        <property type="protein sequence ID" value="ENSXCOP00000022791.1"/>
        <property type="gene ID" value="ENSXCOG00000017032.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155781"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045129">
        <property type="entry name" value="RNF123/RKP/RSPRY1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057987">
        <property type="entry name" value="TPR_RNF123/RKP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13363:SF5">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13363">
        <property type="entry name" value="RING FINGER AND SRY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25576">
        <property type="entry name" value="TPR_RNF123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="E3 ubiquitin-protein ligase RNF123/RKP TPR repeat" evidence="4">
        <location>
            <begin position="142"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="348" mass="40254" checksum="B3CDB762D13BD376" modified="2018-12-05" version="1">MNFFLSGYEETLTQLAAILAKHFADPRIVGTDIKDSLMQALASYVCYPQSLRAVERIPEEQRVAMMRNLLAPYEQRPWAQTNWILVRLWRGCGFGYRYTRLPHLLKTKPEDANLPSLQKPCPSLLLQRHMAELLSMDKDMAASFLNSVLNQLNWAFSEFIGMIQEIQQAAERPERNFVDTRQLKVCATCFDLSVSLLRVLEMTVTLVPEIFLDWTRPSAELLLRRLAQLLNQVLNRVTAEKNLFDRVVNLRLPGLESVDHYPILVAVTGILVRILVDGDKQGKFCKMIFELETSDKVNVCFHRSCKRYLVIVLSIFLPEINRIFYVSLTVELEPEHMQPFLENFGFRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQB9</accession>
    <name>A0A3B5MQB9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQB9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000021684"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021948.1">
        <property type="protein sequence ID" value="ENSXCOP00000021684.1"/>
        <property type="gene ID" value="ENSXCOG00000016213.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157492"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00265">
        <property type="entry name" value="MADS_MEF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000001">
        <property type="entry name" value="Myocyte-specific enhancer factor 2A homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022102">
        <property type="entry name" value="HJURP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033896">
        <property type="entry name" value="MEF2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945">
        <property type="entry name" value="MADS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945:SF25">
        <property type="entry name" value="MYOCYTE-SPECIFIC ENHANCER FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12347">
        <property type="entry name" value="HJURP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="8">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="218"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="405"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="414"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000021684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="464" mass="49436" checksum="77DD74831C469B73" modified="2018-12-05" version="1">MGRKKIQIARIMDERNRHVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSTNKLFQYASTDMDKVLLKYTEYNEPHESRTNSDIVDTLRKKGLNGCESPDIEADDSAGQSPELDDKYCKLNEDIDLMINRQRICGLPPSGYDMGVTIPGSNPSGLLYSHQAIGGAVGNHNLLPISHSSLQRNTMSPQRPCSTGNAGLMASDLPNTVVSSVGNGNFSSHCSSPGLMSPASVSKNMQDKSPPHMGMSHKPDLRTLMPPSNKCSIMPTINKNETTLHNQRINHSQTAQTLSTPAVSIAASGIGGYPSAISSYSTEFSLGSDLSSLSGFGGSGLGSVASWQQQQLQNLQHSALGHMGNFCQNSGLDVGVHNNLHIKCEPASPPRDRSLGVGGAVGGAGLRGGVNTAGYALGTGRGPHESGSSPGDSASSCGSSYEGSEEREDHHGNESFLLQPLHNALLHKPAGVFRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MQY3</accession>
    <name>A0A3B5MQY3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="17">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C19 family. USP20/USP33 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MQY3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000025916"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026227.1">
        <property type="protein sequence ID" value="ENSXCOP00000025916.1"/>
        <property type="gene ID" value="ENSXCOG00000019364.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157311"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2230.10:FF:000001">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000065">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2230.10">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF32">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00695">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="12 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 17" id="KW-0645">Protease</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="13 17" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="11 17" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="20">
        <location>
            <begin position="7"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="19">
        <location>
            <begin position="144"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="DUSP" evidence="21">
        <location>
            <begin position="632"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="domain" description="DUSP" evidence="21">
        <location>
            <begin position="737"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="246"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="836"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="275"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="846"/>
            <end position="857"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000025916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="857" mass="96199" checksum="CAC3B064BD7D0353" modified="2018-12-05" version="1">MPPASSCDCPHLDCVGEITKEELIQKSNGHCQDCKVRGPNLWACLENGCAYVGCGESHSDHSTVHSQETRHNLTVNLTTLRVWCYACGKEVFLERKLGPHSPNAIDNSKAPGSPSSLKLPSHVGYEDLDMETEEEDNLRARGLTGLKNIGNTCYMNAALQALSNCPPLTQFFLECGGLVKTDKKPALCKSYQKLVSDLWHKNRPSYVVPTNLFQGIKAINPMFRGYSQQDSQEFLRCLMDQLHEELKESVPEPSDLSTSIAMDDSPEEDNHSQSDNDFQSCESCGSSERADNEVQGGSIVMDNTNEAEMLIPEQDEIQANREWQKEKNMINDLYRSGFNGVMGGNNGDIDKDVDTTTETMPIISSQGALKVQGRTSAVTTFSPLKNKRQRKYRSVISDVFDGTIVSSVQCLTCDRVSVTLENFQDISLPIPGKEDLAKLHSASHQTSLVKAGSCGEAYAAQGWIAFVMEYIRRFVWFWGPVVTLQDCLAAFFARDELKGDNMYSCEKCKKLRNGVKFCKMQNLPEILCIHLKRFRHELMYSTKIGTHVSFPLEGLDLQPFLAKDSSAQTTSYDLLSVICHHGTASSGHYIAYCRNSVNNLWYEFDDQSVTEVPELCVQNAEAYVLFYKKSSEDAVKERRRVSGLLSMMEPSLLQFYVSRQWLNKFKTFAEPGPISNDDFLCVHGGVPPNKSSYIDDLVVMLPQNVWDHLYSRYGGGPAVNHLYVCHTCQIEIEKLEKRLKSELDLFVRLNKAFQEEESPVVIYCISMQWFREWEGFVKGKDNDPPGPIDNSKIAVNKNGHFILKQGADSGQISEETWNFLHSIYGGGPLVMVRPSVSHQEAESSQSEEKIEVETRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MRL3</accession>
    <name>A0A3B5MRL3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MRL3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027049"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027382.1">
        <property type="protein sequence ID" value="ENSXCOP00000027049.1"/>
        <property type="gene ID" value="ENSXCOG00000020204.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175594"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005154">
        <property type="term" value="F:epidermal growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007173">
        <property type="term" value="P:epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045840">
        <property type="term" value="P:positive regulation of mitotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10740:SF15">
        <property type="entry name" value="EGF-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10740">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 3" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="5">
        <location>
            <begin position="5"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="3">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000027049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="96" mass="10820" checksum="ECE9E5DC12CE92CA" modified="2018-12-05" version="1">MMAEHGKPCDPQEATFCMNEGTCYKLSAMNTLSCICSENYKGNRCEQYHLPSSSQDGYDRGLLAAIIVVAIILLVVLVVIIYYKCQCQFGFFQPRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MSN9</accession>
    <name>A0A3B5MSN9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="4">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DNA polymerase type-B-like family. GLD2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MSN9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000022514"/>
    <dbReference type="Ensembl" id="ENSXCOT00000022789.1">
        <property type="protein sequence ID" value="ENSXCOP00000022514.1"/>
        <property type="gene ID" value="ENSXCOG00000016821.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156640"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000007">
        <property type="entry name" value="poly(A) RNA polymerase GLD2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002058">
        <property type="entry name" value="PAP_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271">
        <property type="entry name" value="POLY A POLYMERASE CID PAP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12271:SF40">
        <property type="entry name" value="POLY(A) RNA POLYMERASE GLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03828">
        <property type="entry name" value="PAP_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="12">
        <location>
            <begin position="83"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="PAP-associated" evidence="11">
        <location>
            <begin position="306"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000022514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="401" mass="45920" checksum="62A9F3E8D91C129C" modified="2018-12-05" version="1">MLTFASCRRHHNEYNAHAVKRQRLNPSPASSPRWIPHNNLAVGEPPMSAPVGFEHFHSSPPITEVLSEPQNLTSLQVFVKDKLSGQMVELFEACQQQTSDLDRKEACRARLQGDIQKQFPESRLYLTGSSMSGLGCRSSDADLCLVIKGNVRLRRRPMDVLRQLHKDFRSLSYLARIQLIKAKVPILKFKERSSFLEFDLNVNNTVGIRNTFLLRTYAYADIRVRPLVLVVKKWAGHHQINDASKGTLSSYTLVLMVLHYLQTLEQPVLPSFQNTHPECFDLLMDIDEVPDGPKHVRLFTSTNQLSLGELLLGFLRYYATRFSWDKQVISVRQATTLPKTNSKEWRNKFICVEEPFEGSNVARAVHEKTKFDTIKKCFSERKDLVSILALRAIINEELATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MUS4</accession>
    <name>A0A3B5MUS4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MUS4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023244"/>
    <dbReference type="Ensembl" id="ENSXCOT00000023522.1">
        <property type="protein sequence ID" value="ENSXCOP00000023244.1"/>
        <property type="gene ID" value="ENSXCOG00000017360.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005772"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031630">
        <property type="entry name" value="CCDC117"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36128">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36128:SF1">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15810">
        <property type="entry name" value="CCDC117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000023244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="211" mass="23755" checksum="2B1B2F5C2DC1B702" modified="2018-12-05" version="1">WETRRFRKHRRLDDGCSAKKRRLMPETEVDILVNTCPTLSAQQVPLPPPPQPCSAHQSLTLLQPSSVQPRAEMEIPCMEIEAAHRKLQEIEDIILEDDDEDLDVEPVPRRPVLVISDSLKEGLQRGISDILPQTVAQSPSGMELVLWRPPEDPFCQRLKDSLQKQRKQQTVGRQHPTPCSSPSPPLSPSCPPAETCAPMYSFPVLHHSGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MW76</accession>
    <name>A0A3B5MW76_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MW76"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000023864"/>
    <dbReference type="Ensembl" id="ENSXCOT00000024148.1">
        <property type="protein sequence ID" value="ENSXCOP00000023864.1"/>
        <property type="gene ID" value="ENSXCOG00000017825.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157553"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04273">
        <property type="entry name" value="ZnMc_ADAMTS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
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        <property type="entry name" value="ADAM metallopeptidase with thrombospondin type 1 motif 9"/>
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        <property type="entry name" value="semaphorin-5A isoform X1"/>
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        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
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        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
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        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
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        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
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        <property type="entry name" value="ADAMTS_CR_2"/>
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        <property type="entry name" value="ADAMTS_CR_3"/>
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        <property type="entry name" value="ADAMTS_spacer1"/>
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        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
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        <property type="entry name" value="Peptidase_M12B"/>
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        <property type="entry name" value="Peptidase_M12B_N"/>
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        <property type="entry name" value="PLAC"/>
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        <property type="entry name" value="TSP1_rpt"/>
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        <property type="entry name" value="TSP1_rpt_sf"/>
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        <property type="entry name" value="A DISINTEGRIN AND METALLOPROTEINASE WITH THROMBOSPONDIN MOTIFS 18"/>
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    <dbReference type="PANTHER" id="PTHR13723">
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    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="15" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="12 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 15 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="10 15 17" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M12B" evidence="19">
        <location>
            <begin position="231"/>
            <end position="434"/>
        </location>
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            <begin position="1095"/>
            <end position="1132"/>
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    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
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        <location>
            <position position="375"/>
        </location>
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        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
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        <location>
            <position position="234"/>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>2</label>
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        <location>
            <position position="323"/>
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        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
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        <location>
            <position position="378"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <note>catalytic</note>
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        <location>
            <position position="383"/>
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            <name>Zn(2+)</name>
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            <name>Ca(2+)</name>
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            <label>1</label>
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    <sequence length="1133" mass="126106" checksum="3E8B87AF95F61FC7" modified="2018-12-05" version="1">YVFVTPVEVDSDGTYLTHDVTRRGRRSRRSLSLSLHYRLSAFGQDMYLDLLPSSVVGPGFTVQTVRSDGISTVIGDEGLHHCLYQGFIRNVSASLAAISTCSGLAGLIRVSNEEYLIAPLPQHLADRHNYSAPYGHHPHVVYKRSAEHVVHRRSSSDENPYRSHHQHQAQTHHDYQHGKLQRQHFCGRRKQAPQPPVGDLFILPDEFAAPEVEEPRRARRSPINSNRMGGLNVETLVVADRKMLEKHGRENVTTYVLTVMNMVSSLFKDGTIGSDINIVVVSLLLLEHDAQLGLNINHHADQSLNSFCQWQSGLVGKGGKRHDHAVLLTGLDICSWKNEPCDTLFAPISGMCSKYRSCTINEDTGLGLAFTIAHESGHFGMIHDGEGNPCRKTEGNIMSPTLAGNNGVFSWSTCSRQYLSRFLTAQASCLGDEPKQIGQYKYPEQLPGQLYDADTQCKWQFGSQAKLCGLDFVKDICKSLWCHRVGHRCETKFMPAAEGTTCGPDMWCRRGQCVKYGEHGPRSVHGQWSAWSQWSDCSRTCGGGVMFRERSCTNPPQNNGKFCPGSTRLNQLCNTRPCPSNALDFRAQQCAEYNSKPFRGWYYKWKPYTKVDEDICKLYCIAEDFDFFFAMSSKVKDGTSCSDHREGVCIDGAVGCDRLLGSKASLDACGVCKGDNSTCKFFKGQYTLQHRANYYSMVTVPAGARGIHIQEMEVSTSYLAVRSLKKKYYLTGDWTVDWPGRFYFGGTVFDYQRSFNKPESLYAIGPTNETLVFEILLQGKNPGVVWEYTLPRTERKPEYSWGVVRSDCSAPCAGRISSKAICLQDHRTQANSSMCNPHTRPALGSHLCNTQPCPAWAPGGWGPCSRSCGGGQQTRALRCMRKVTYQRDEAAAHSLCPVISPAQIQPCHIQACPPEWSSGSWSQCSKSCGRGLRKRTVFCRSTDPGAHAVVVPDSMCRQHHRPKAQETCVLRRCPRNDRLQWIPTPWGEEYPVRRCRNTAKPSLDLQQVCSAAPCPERPRVVPGRTAAGASVSGWFPSPWQQCSVSCGGGVQTRTVQCLQQGRPQAGCPAHQRPVTSRACNTNFCPAAPPGPAPRLDEHCVDHFNWCHLVPQHGVCSHRFYGQQCCKSCSSKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MWU4</accession>
    <name>A0A3B5MWU4_9TELE</name>
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        <recommendedName>
            <fullName evidence="7">Dehydrogenase/reductase (SDR family) member 13b.1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000024264"/>
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        <property type="protein sequence ID" value="ENSXCOP00000024264.1"/>
        <property type="gene ID" value="ENSXCOG00000018110.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="SDR_fam"/>
    </dbReference>
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        <property type="entry name" value="DEHYDROGENASE_REDUCTASE (SDR FAMILY) MEMBER 13-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017457934" description="Dehydrogenase/reductase (SDR family) member 13b.1" evidence="4">
        <location>
            <begin position="19"/>
            <end position="458"/>
        </location>
    </feature>
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        <source>
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        <source>
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        </source>
    </evidence>
    <sequence length="458" mass="50660" checksum="505538DCBDD58DCB" modified="2018-12-05" version="1">MYAIVLLVGFLIAAYVFHETVVKGKSCTSKAKLHGKTVIVTGSNTGIGKTMNLAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQNNDSESGSSQVVFMQLDLASLKSVRSFAENFLKTEPRLDILINNAGLFNFGRTEDGFGMTFGVNHLGHFLLTNLLLDRLKECGPSRVVNVSSGAHNWGRVDFDCLNTHKTLRTGTSFVDGQRVYADSKLCNVLFTYELAKRLKGTKVTCYSLHPGKNASPTFQLVALNGLRFFMKTPLQGSQTTLHCALHEGIEHLSGRHFSNCTARDVLAKAKDPVTAKKLWEISERFCDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MYG0</accession>
    <name>A0A3B5MYG0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5MYG0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026977"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027310.1">
        <property type="protein sequence ID" value="ENSXCOP00000026977.1"/>
        <property type="gene ID" value="ENSXCOG00000020150.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175655"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="85" mass="8925" checksum="95F8979E3C41766A" modified="2018-12-05" version="1">MSSVCVLKRKAVLWQDSFSPHHRSTSPSMPVVLSSGGHALPTGQTPQATPPTLPGLLGDGTGSLGLLGNSVLRINLTQHLVKFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MZM6</accession>
    <name>A0A3B5MZM6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">purine-nucleoside phosphorylase</fullName>
            <ecNumber evidence="3">2.4.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Inosine-guanosine phosphorylase</fullName>
        </alternativeName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2'-deoxyguanosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:27738"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17172"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2'-deoxyinosine + phosphate = 2-deoxy-alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27750"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:28997"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>guanosine + phosphate = alpha-D-ribose 1-phosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:13233"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:16750"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>inosine + phosphate = alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27646"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PNP/MTAP phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MZM6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000026060"/>
    <dbReference type="Ensembl" id="ENSXCOT00000026373.1">
        <property type="protein sequence ID" value="ENSXCOP00000026060.1"/>
        <property type="gene ID" value="ENSXCOG00000019467.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182991"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004731">
        <property type="term" value="F:purine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011268">
        <property type="entry name" value="Purine_phosphorylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904">
        <property type="entry name" value="METHYLTHIOADENOSINE/PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904:SF12">
        <property type="entry name" value="PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="11">
        <location>
            <begin position="9"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000026060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="76" mass="8620" checksum="327865DE53AAAFD1" modified="2018-12-05" version="1">TKTFPNFPTTVHEVIVARHAGMRCFAMSLVSNRAVMDYDSREKANHDEVLETGQQRAKQMEVLVSTMVTLKPDKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5N176</accession>
    <name>A0A3B5N176_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 19">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="13">IMP dehydrogenase</shortName>
            <shortName evidence="13">IMPD</shortName>
            <shortName evidence="13">IMPDH</shortName>
            <ecNumber evidence="13 19">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">IMPDH</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13 19">
            <text>IMP + NAD(+) + H2O = XMP + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="13">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11 13 19">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 18">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="13 19"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5N176"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000027275"/>
    <dbReference type="Ensembl" id="ENSXCOT00000027608.1">
        <property type="protein sequence ID" value="ENSXCOP00000027275.1"/>
        <property type="gene ID" value="ENSXCOG00000020362.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154156"/>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04601">
        <property type="entry name" value="CBS_pair_IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000007">
        <property type="entry name" value="Chromosome 19 SCAF14664, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01964">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01302">
        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911:SF74">
        <property type="entry name" value="INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="INOSINE-5-MONOPHOSPHATE DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="1 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 13 19" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="2 13 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13 15" id="KW-0520">NAD</keyword>
    <keyword evidence="10 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 13 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 13 16" id="KW-0630">Potassium</keyword>
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    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="20">
        <location>
            <begin position="179"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="20">
        <location>
            <begin position="244"/>
            <end position="302"/>
        </location>
    </feature>
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        <location>
            <position position="396"/>
        </location>
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        <location>
            <position position="493"/>
        </location>
    </feature>
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        <location>
            <begin position="339"/>
            <end position="341"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <begin position="389"/>
            <end position="391"/>
        </location>
        <ligand>
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        </ligand>
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        <location>
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        </location>
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        <location>
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        </location>
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        </ligand>
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        </location>
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            <name>IMP</name>
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        </ligand>
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        <location>
            <begin position="429"/>
            <end position="431"/>
        </location>
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        </ligand>
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        <location>
            <begin position="452"/>
            <end position="453"/>
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            <end position="480"/>
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        </ligand>
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        <location>
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            <dbReference type="ChEBI" id="CHEBI:29103"/>
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        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        <source>
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    </evidence>
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            <dbReference type="RuleBase" id="RU003927"/>
        </source>
    </evidence>
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            <dbReference type="RuleBase" id="RU003928"/>
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            <dbReference type="PROSITE" id="PS51371"/>
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    </evidence>
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            <dbReference type="Ensembl" id="ENSXCOP00000027275.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="572" mass="62571" checksum="718512AED811D49E" modified="2018-12-05" version="1">MEGHGYDRFGESDRDNYQIDYRRIVGDMEPARPRVSNRDGEQSQHSFLTHAYPPSYGHGHETAAQRYSATRIQAGYEPESMADYLISGGTDFTEAHTLDFLILPGFIDFTSDEVDLTSALTRKITLKTPLISSPMDTVTESSMAITMALMGGIGIIHHNCTAEFQANEVRRVKKFEQGFITDPVVMSPHHTVGDVFEAKRRHGFSGIPVTETGKMGSKLVGIVASRDIDFLSEKDYEKLLEEAMTKREDLVVAPAGVTLKEANDILQRSKKGKLPIVNDNDELVSIIARTDLKKNRDYPLASKDSRKQLLCGAAIGTREDDKYRLDLLMQAGVDVVVLDSSQGNSVYQINMINYIKQKYPDLQVIGGNVVTAAQAKNLIDAGVDALRVGMGCGSICITQEVMACGRPQGTSVYKVAEYARRFGVPVIADGGIQTVGHVVKALALGASTVMMGSLLAATTEAPGEYFFSDGVRLKKYRGMGSLDAMEKNNSQKRYFSEGDKVKVAQGVSGSVQDKGSIQKFVPYLISGIQHGCQDIGARSLSILRSMMYSGELKFEKRTMSAQMEGGVHGLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KN88</accession>
    <name>A0A3B5KN88_9TELE</name>
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        <recommendedName>
            <fullName evidence="7">IGFBP N-terminal domain-containing protein</fullName>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KN88"/>
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        <property type="gene ID" value="ENSXCOG00000001278.1"/>
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        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:extracellular space"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
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        <property type="entry name" value="IGFBP-like"/>
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        <property type="entry name" value="IGFBP_1-6_chordata"/>
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        <property type="entry name" value="Insulin_GF-bd_Cys-rich_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11551">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN"/>
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        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN 2"/>
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    <dbReference type="Pfam" id="PF00219">
        <property type="entry name" value="IGFBP"/>
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        <property type="entry name" value="IGFBPFAMILY"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Growth factor receptor domain"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0341">Growth regulation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
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            <begin position="18"/>
            <end position="122"/>
        </location>
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            <begin position="19"/>
            <end position="99"/>
        </location>
    </feature>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023183"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="122" mass="13010" checksum="1A4CA3217481EA25" modified="2018-12-05" version="1">MFGLLLAVLALPGFLRGDLVFRCPGCTAENQDGCPKVTLCAEIVREPGCGCCPVCARLEGEFCGVYTPRCSTGLLCSPSMDAELPLQQLIQGFGRCTRSVEQDPSLDLLPTNGLVSEEQRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQB9</accession>
    <name>A0A3B5KQB9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        </lineage>
    </organism>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSXCOP00000000060.1"/>
        <property type="gene ID" value="ENSXCOG00000000047.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166296"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000999">
        <property type="entry name" value="Si:dkey-8e10.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359:SF34">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24359">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SBK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="32"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="310" mass="35110" checksum="A126901F2C5563DA" modified="2018-12-05" version="1">MIELGLAEGCLIDELMELTSQSIGQLEIQEHFNIIKEIGRGKYGKVLLVTHTPMALKVMPKASTKLQGFLREYCISLHLSCHPCIVGLFGIAFQSKEHYCFAQELVIGRDLFAVIKPKVGIPESSVKRCVLQISSALEFIHSHGLVHRDVKPENILLLDTHCRQVKLADFGLAQKRGTLIRFITGTLPYMAPELCSVALLDNQKEVTVPPLSVEPSLDTWAFAVVIYCILTGYFPWERCMDSDSFYQEFADWCETKKKVYVEDDVPPLWRRFTPEAMEMFSLLLAPDASQRSSAGAVRAYVEKDWLRKDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KQL9</accession>
    <name>A0A3B5KQL9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MICOS complex subunit MIC10</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 9">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Mitochondrion inner membrane</location>
            <topology evidence="2 9">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the MICOS complex subunit Mic10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KQL9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002418"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002452.1">
        <property type="protein sequence ID" value="ENSXCOP00000002418.1"/>
        <property type="gene ID" value="ENSXCOG00000001932.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000168345"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007512">
        <property type="entry name" value="Mic10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21304">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21304:SF0">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04418">
        <property type="entry name" value="DUF543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="78" mass="8611" checksum="017933FC8BA87F55" modified="2018-12-05" version="1">MSEKELGKKLDRCLADGVVKLGTGLGLGIVFSVLFFKRRTWPITLFSGAGLGMAYANCQHDLRSHYLLHTKVQSVRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRB2</accession>
    <name>A0A3B5KRB2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Keratin 92</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRB2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002658"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002693.1">
        <property type="protein sequence ID" value="ENSXCOP00000002658.1"/>
        <property type="gene ID" value="ENSXCOG00000001910.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182969"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF367">
        <property type="entry name" value="KERATIN 15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="5">
        <location>
            <begin position="149"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="21"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="443"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="146"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="235"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="347"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="447"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="474" mass="51491" checksum="6C52E6B1BCCBFD25" modified="2018-12-05" version="1">MEQQNKVPDIPAAVCHRPAAAVGGGVTRTPPHPLQQHKGGSARAPLHSPLLHPEQQNSSSMASFFSTRSSSYSSGGRLGSMRSGSVYGGAGGSGVRISSSTASRNFSSGGGAGFAAGGGGAGFASGGGAGFAAGGGFNLSDALDISDNKKAAMQNLNDRLASYLEKVRSLEAANADLEKKIRDNQTQKRCFKSRLSSSQIIDATRVNGSLHLSIDNAKLATDDFRIKYENELAMRQSVEADIAGLKRLLDELTLARSDLEMQIESLREELIYLKKNHEEVSRADMRSQVGGQVNVEVEAAQQPDLTAMMAEIREHYEGVASKNRKDLDNWFQSEELQKETAVSTETLQTSRSEISDVKRTLQNLEIELQSQLTSLEATLAETQSRYSSMLGGYQMQVTRLEEQLMQLKADLERQRQEYQMLLDMKTRLEMEIAEYRRLLDGEGGGSLSSCLSSNSSCSGRGRLENPDEPEPVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KRV0</accession>
    <name>A0A3B5KRV0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KRV0"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001224.1">
        <property type="protein sequence ID" value="ENSXCOP00000001207.1"/>
        <property type="gene ID" value="ENSXCOG00000000990.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000176964"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="154" mass="17037" checksum="455FE96FF6CFA7BE" modified="2018-12-05" version="1">IMETKLGQDIFQLLSGNDDVVQELLFKMIKSENPIVIYKLTECFRRTLARPRKIHLKKNYISKVWKWIEQAGPPDIQGPPDTQGPPDIQGPPETQGPPDIQGPPDTQGPPEIQGPPDTQGPPDIQGPPDTQRPPDTQGPPRNGLIVTQTTDLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KS99</accession>
    <name>A0A3B5KS99_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transporter</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KS99"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000506"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000515.1">
        <property type="protein sequence ID" value="ENSXCOP00000000506.1"/>
        <property type="gene ID" value="ENSXCOG00000000438.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154583"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005332">
        <property type="term" value="F:gamma-aminobutyric acid:sodium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005369">
        <property type="term" value="F:taurine:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616:SF141">
        <property type="entry name" value="SODIUM- AND CHLORIDE-DEPENDENT TAURINE TRANSPORTER"/>
        <property type="match status" value="1"/>
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    <sequence length="604" mass="67959" checksum="B64CF8959807951E" modified="2018-12-05" version="1">MAQKEKLQCLKDFHKDTLKPSPGKSPGTRPEDEAEGKHPQREKWASKLDFVLSVAGGFVGLGNVWRFPYLCYKNGGGAFLIPYFIFLFGGGLPVFFLEVALGQFTSEGGITCWEKLCPIFTGIGYASIVIVSLLNIYYIVILAWGLYYLFQCFQPELPWAKCNQPWNTDQCIEDTYRKNKTIWVAANASNFTSPVTEFWEHNVLGISSGIEEIGPVKWDLALCLLLVWVICFFCIWKGVKSTGKVVYITATFPFVMLIVLLIRGVTLPGASEGIKFYLYPDLERLKDPEVWIDAGTQIFFSYAICLGAMTSLGSYNKYKYNCYRDCLLLGALNSGTSFVSGFAIFSVLGFMAQEQGVDIAEVAESGPGLAFIAYPKAVTMMPFPTLWAILFFIMLLLLGLDSQFVEVEGQITSLVDLYPSFLRKGYCREVFIAIICCISYLFGLTMVTKGGMYVFQLFDYYAASGVCLLWVAFFECIAVAWVYGADNFYDAIEDMIGYRPNPWMKWSWTVVTPLLCMGCFIFSLVKYKPLTYNKMYQYPDWAIGIGWTLALTSMICIPMVVVIKIIRGSRPWRLRRVEGPAPAPRTTVSPTSSPAHWTPMETRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KSI9</accession>
    <name>A0A3B5KSI9_9TELE</name>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
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            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
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            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
    </reference>
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            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="11">Belongs to the chemokine-like receptor (CMKLR) family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
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    </dbReference>
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        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="293" mass="32950" checksum="2D0BF8BE7675F4DB" modified="2018-12-05" version="1">MQHKMTRKPGHFGSFNLIPPIQPIQIVSLVLYALVVLLGVPGNALVLWVTGFCMNRSVTSIWFLNLALTDLLCCLSLPLLMVPLAHDDHWHFGSLACTLVKGLFYLVMYASVLQLVLISVDRLLLVKKAVWCQNNRRPRYAAWACVAVWCLALLGSIPQFVYARLIEKGELKRECLLAYSKESVCTTFIVVVVLSFFLCWLPVHIMDFIDLATPLSSSHSPRIYMAQVLTVCLAYFNSCLNPLIYVCVGRSFKDSMNRSLRHILHFATEDPASRGSISNNTKSTINERVITTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KT59</accession>
    <name>A0A3B5KT59_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KT59"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003293"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003331.1">
        <property type="protein sequence ID" value="ENSXCOP00000003293.1"/>
        <property type="gene ID" value="ENSXCOG00000002594.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158109"/>
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        <property type="component" value="Unplaced"/>
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    </evidence>
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    <sequence length="129" mass="13683" checksum="1EF898B7E0ABF338" modified="2018-12-05" version="1">AFPLVSSLSSGSDGQNVVVTQSAAKSVQLGQTVTIDCKASRQVACNTPCLSWYHQKSGEVPKALIYRTSSRFSGISSRFSGSGARNKLDFTLTISGVQAEDAGVYYCQSYHDINSQDVKNNPGGSLLLA</sequence>
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<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
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        </source>
    </evidence>
    <sequence length="1641" mass="181896" checksum="0B01CBE2AEC6700F" modified="2018-12-05" version="1">EATQNQIKELHRRLQQMETQNTGLSSENNRLKKNISALLRTARQEIIRKDAEIKQLTQHSQFEEEPPPADTPQPTSKETCHFSRSMKSSTHNCSKRHETAEKLPGVEGCTSRDREVKGQSNKLSESAERKLRDCSHHNKDSEQKQAHKEDKDTRKGFDSQSHKTKKYQNVGRHCRSDGAKTSSQEHLHSVASSEHRREGRKIQRTDKASSIEHAKVSDQQSGGSCSPESRKIPRGHKYDSWSNPKNKRSSSPDQSTKCCSDGSRERERSRQRDHQRSVDRRCEDEIRSRHHRRSSQKESSRDREKHRDKQTGKADGSKEGRRETKPRDVKRSFEEPSPDANTSVNDNNSNRKLCFMETLNLTLSPIKKYTMPRDSSLEEPLRLEGDDEENSQPNLDNMCVIDEVDGGELGSEPGPGHAEEQALEELKAPRNQTSNLEDVEEDGNNGAESQGRPLAVLISSLWRPPQGPADTTKLHTAAGENLKHPEEPLELISNTWITERGQLEVTKSSQPSNTPPQILEQHSSSEAPTSEVQHAAAADEHADPHAHAAQHEHAAAADEHADPHAHAAQHEHAAAADEHAAADSGEAPAPPRLSQSTVSEAAASAQPDDTHGTEEVYEEARLRDGWGHQAASPGTSLPSSTSVTMDNHPVGQPQSYKDLDSVSSTICLTSLPQKGLSLTEDICAMTQTDTDPRTSSSQPSSSVDCIGVSKVSSTTEELSPATVTPKKFCSQDCKKELSTSVSLPHDEDSMMHTLSNLKRIPEVISPLRSPAQTAKRGVLHVHSKLGHVKSLQKEFSTSAAETNLMKLDVNKENKYPGSPANRGAQDMVNMESEQISSPFDTELEEGEILSESDEATSSSPVPATKRAKLTEPVRNKPSLQSSSRRKPEEQRVASKEPLDSAAVSTQSPRSRFKTVCPSASKASFSTVEEIMETFKMVRTEMRKKYMKLHKTFPKKSFWGVMDNFQKSFLEFVDGAHFGQICSDAEELKSKLRKLITSVFSKVLNNGIVKRIFEQQAVNLKQKLWDFVDVQVDYLLMDIQTTLKSLCQAVRIPTEDKRPGGQGTEPQQAPVKKPLCKQNKTRIQCPEHPPNSSLGRTKSCAVVPYRTGLGSRGKDIRMSYTEKDGASHIHPPGRVNTQTTVNFLSDSNAALSPDKTKMVIPQNGSVIDRTDFELLTEQQASSLTFNLVRDSQMGEIFKCLLQGSDLLESTGMCGDNTAWAVGTPRKDGERFLNFATPSKFLSPSKSDTSARLIATWSTISPRRRSSPCIKTPIPLHPALFDESCLLEVPSGNRALLQGSLATEKYSILAEDLAVSLTIPSPLKSDSHLSFLQPAGVGIHVVSTPDSVISAHISEDALLDGEDATEQDIHLALDNSSCSSRDSTTSQTPGSTLFFKPDVPMQALVMEKSNDHFIVKIRQMNVAAEGTLIAGEPLRQTLIEKNQQHEEHDAQERLKVLQVEATPPKSFPFQSQKQTSPPSATLSPNTKTPFYDSGREETTVSESEKSLTIDTSSSSEKTSRDCEQLRKRKRRQEKLKAKRCKKEDKSLQEVSPSRKSDEELKLSPAALSPSSLSAKNIIRKKGEVVMAWTREEDRAILMFLKTKGASRETFSTLSEKLNKPSGQIAHRFYQLMKLFKKQGKMDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KVS3</accession>
    <name>A0A3B5KVS3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Complexin-2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">Binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Perikaryon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Presynapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the complexin/synaphin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KVS3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000001711"/>
    <dbReference type="Ensembl" id="ENSXCOT00000001736.1">
        <property type="protein sequence ID" value="ENSXCOP00000001711.1"/>
        <property type="gene ID" value="ENSXCOG00000001388.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182938"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046928">
        <property type="term" value="P:regulation of neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016079">
        <property type="term" value="P:synaptic vesicle exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22740">
        <property type="entry name" value="Complexin_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.580:FF:000001">
        <property type="entry name" value="Complexin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.580">
        <property type="entry name" value="Single Helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008849">
        <property type="entry name" value="Synaphin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16705">
        <property type="entry name" value="COMPLEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16705:SF9">
        <property type="entry name" value="COMPLEXIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05835">
        <property type="entry name" value="Synaphin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="8" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="13"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000001711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="118" mass="13636" checksum="9BF637606D1E95DF" modified="2018-12-05" version="1">MGKMLGGEEEKDPDAAKKEEERQEALRQQEEERKAKHARMEAEREKVRQTIRDKYGLKKKEEKEAEEKAAMEQACEGSLTRPKKAIPRGCGDDEEDEESILDTVLKYLPGPLQDMFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXJ5</accession>
    <name>A0A3B5KXJ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translation initiation factor eIF2 assembly protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Cell division cycle protein 123 homolog</fullName>
        </alternativeName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">ATP-dependent protein-folding chaperone for the eIF2 complex. Binds to the gamma subunit of the eIF2 complex which allows the subunit to assemble with the alpha and beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDC123 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXJ5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002351"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002385.1">
        <property type="protein sequence ID" value="ENSXCOP00000002351.1"/>
        <property type="gene ID" value="ENSXCOG00000001684.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003057"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009772">
        <property type="entry name" value="CDC123"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15323:SF6">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 123 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15323">
        <property type="entry name" value="D123 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07065">
        <property type="entry name" value="D123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="50"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00044080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="359" mass="40620" checksum="E4D4BA60CFE87D3E" modified="2018-12-05" version="1">MKKEQVVHCQFSVWYPIFKKHTIKSLILPLPQNVIDYLLDDGTLVISGSDHNSQPIQTNNSESDAEEDVQRNNCDSFSKAPEFPEFASTVLEAINALGGCVFPKLNWSAPRDANWIALNSSLECRSLGDVFLLFKSSDFITHDLTCIPGLDGNGWCPCPQLVLRKWAELIPGGEFRCFVKENKLIAVSQRDHTQYYQHILKQEQHISQAIQDFFSQHVQYRFLDEDFVFDVYRDSQGRVWLIDLNPFGEVTDSLLFSWDELMSGERTAFDGPAFRYTTSEVTVQPSPCLSYRIPRDFVDLSAGEDAYKLIDFLKLVGSGVCTLENATDKNLKAVSKLAKKIACTPVQYWAGADAKVLIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KXR0</accession>
    <name>A0A3B5KXR0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KXR0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000000344"/>
    <dbReference type="Ensembl" id="ENSXCOT00000000351.1">
        <property type="protein sequence ID" value="ENSXCOP00000000344.1"/>
        <property type="gene ID" value="ENSXCOG00000000171.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153700"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001473">
        <property type="entry name" value="Casein kinase I isoform alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="17"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="320"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="339"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000000344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="349" mass="39897" checksum="601D670F21744D6C" modified="2018-12-05" version="1">MASSSGSKAEFIVGGKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQKARHPQLLYESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDLFNFCSRRFTMKTVLMLADQVSKIALINPNPQISECDERRCRKYPVLLPRSYFRPLAYGSPDLRSSVSWSIFSPPTPTSYVRLLTWPLPPPPQAPHSREHFPYFQTQNLTGMGRRDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTMLKQKAAQQGASSGGQGQQAQTPTGKQTDKSKPNLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KY40</accession>
    <name>A0A3B5KY40_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Organic cation transporter 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KY40"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005023"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005083.1">
        <property type="protein sequence ID" value="ENSXCOP00000005023.1"/>
        <property type="gene ID" value="ENSXCOG00000003941.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155089"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="14973" checksum="17D9083C4ED73C55" modified="2018-12-05" version="1">MANVDKLTEHIGDFGPFQKKMVVLGSLPLVLIAFVFVGVVFLGHTPDHWCWSPGSEQLLQECGWTEVKVREVTVPHGEEAGSFSRCQAFALNLSQSRDKCEEFEQALTLNAVPCDGRWMFDKSHKTTISELGTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KYT4</accession>
    <name>A0A3B5KYT4_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5KYT4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005258"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005319.1">
        <property type="protein sequence ID" value="ENSXCOP00000005258.1"/>
        <property type="gene ID" value="ENSXCOG00000004115.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013973"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16155">
        <property type="entry name" value="DED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16155:SF18">
        <property type="entry name" value="STERILE ALPHA MOTIF DOMAIN-CONTAINING PROTEIN 9-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="774" mass="89273" checksum="FFE228626A96274F" modified="2018-12-05" version="1">MQVLWDMRKTLRCAVLMGSPLECTKIAKEVVHLFTGGSKDDLNTVLLLINEEFILEHLQDSIMTEIEEQQIDIDIPVVILFICVRSDHALSQKYGAKASQFHGFNILHSNFSQDYIQEACAVLNMVERKKKPLKTQLLSFLSLLNAYVPGSYLLESDCLDFLRHDNSLQGDLSVVTRKQPFSHLIISFQHDKRCEKRIRMAHPMIAKHCTELLAIAGVARSDTARDFLLQFCPDEVSPCLLGFIKDLLTKREHKKDGSIEDSDLEKFSTLILHINEVEDKNQSALVLKVASEKFDKNSLFPQALARLCYGELEDFKEAEFWAKKAIERDPKKSFIADTLGQVHKNHLRNKRFSIEPRELLQLAQKAIEAFEHEEKLAEEEHRKSLTEDEKSKVLWGLNTRGQFGFLQVYNILFDLLVFQNETWKEVLTKSVPMGSVLESLGDTKLFRFRDLVENLREKVEKKMEFFDNFLTYSQSVLRKDKAYVSREASACYKKYVGDSVPEHNGRLQQTFQKLKQKLAITSAGVLSCLDRTCTSHIEYIIEWWREISQGNNSTTEAFVNFILAQIMLSNKNKAISSSDYKNALKLKKPPSSVTQAEYHLLSLLLSWPTNDEDKPVPHLYQLIKNASHAYEREYKTMFRSRYLRPLFFLGPGISLKRFVHRRVLEILWTKDALQESNTNWKNESIFRDSIVEEHLLKVEGVVRNYGVYARVRGTEVEVDANRRDCLCRSGHVSFYLGFTIKGPVAFSIRRKLLCDVIHVPSVGQDKYFTLQTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5KZ76</accession>
    <name>A0A3B5KZ76_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5KZ76"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005388"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005450.1">
        <property type="protein sequence ID" value="ENSXCOP00000005388.1"/>
        <property type="gene ID" value="ENSXCOG00000004214.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158214"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="122"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="364" mass="42163" checksum="D88ADA24AB7BA127" modified="2018-12-05" version="1">MALSRGLRCCQRVFSWIPVLIITAVVLWSYYAYVFELCLFTISNALEKAVYLLVFHVCFGMFSWTYWKSIFTPPATPCKKFQLSYSDKQRYEMEERPDAQKQILVEIAKKLPIFTRTQSGAIRFCDRCQVLKPDRCHHCSVCETCVLKMDHHCPWVNNCVGFSNYKFFLLFLAYSMLYCVFITATVLQYFLKFWVGDLPNGPAKFHILFLMFVALMFFVSLMFLFSYHCWLVAKNRSTLEAFSAPVFASGPDRNGFSLGLHKNLLQVFGEDRKLWFIPVFTSQGNGHYFPLRSQSSESHNPLLANEDMWDESDDGSEGKAEENNVLGLDSVFYQHPDGLDHRVPCTYRSSRCEVRCALSPAWLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L153</accession>
    <name>A0A3B5L153_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquinone biosynthesis protein COQ4 homolog, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase</fullName>
            <ecNumber evidence="6">4.1.1.130</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Coenzyme Q biosynthesis protein 4 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">COQ4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Lyase that catalyzes the C1-decarboxylation of 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoic acid into 2-methoxy-6-(all-trans-polyprenyl)phenol during ubiquinone biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoate + H(+) = a 2-methoxy-6-(all-trans-polyprenyl)phenol + CO2</text>
            <dbReference type="Rhea" id="RHEA:81179"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10931"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="ChEBI" id="CHEBI:84443"/>
            <dbReference type="EC" id="4.1.1.130"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion inner membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the COQ4 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.130" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L153"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000003611"/>
    <dbReference type="Ensembl" id="ENSXCOT00000003650.1">
        <property type="protein sequence ID" value="ENSXCOP00000003611.1"/>
        <property type="gene ID" value="ENSXCOG00000002844.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003828"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120539">
        <property type="term" value="F:4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03111">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007715">
        <property type="entry name" value="Coq4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027540">
        <property type="entry name" value="Coq4_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ4 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05019">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="1 6" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017319738" description="Ubiquinone biosynthesis protein COQ4 homolog, mitochondrial" evidence="7">
        <location>
            <begin position="25"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000003611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="268" mass="30439" checksum="854E0BD08B5C7CF5" modified="2018-12-05" version="1">RCRFVSGAFLAKTTLLVHRLFTSGAELTYSYDGLYPGHIQTTSFQKALLAVGSGVAALQDPYRHDMVAVLGETTGHLALINLRERMKNDPEGYTILTERPRIRLSTLDLEKMASLPDESFGREYLRFLEDNKVTPDSRAEVKFVDNEELAYIMLRYREVHDLLHTLLGMPTNMLGEVAVKWFEAAQMGLPMCALGAVLGPLQLNASRLQSLFTSLGPWALQNGRRARCVLSIFYERRWEQSLEDLRRELNIEPPPLTLSASNKRRRQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L2V2</accession>
    <name>A0A3B5L2V2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">palmitoyl-CoA hydrolase</fullName>
            <ecNumber evidence="7">3.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.2.2" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L2V2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005132"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005193.1">
        <property type="protein sequence ID" value="ENSXCOP00000005132.1"/>
        <property type="gene ID" value="ENSXCOG00000004015.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155229"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052816">
        <property type="term" value="F:long-chain fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03442">
        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000009">
        <property type="entry name" value="Cytosolic acyl coenzyme A thioester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000010">
        <property type="entry name" value="Cytosolic acyl coenzyme A thioester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040170">
        <property type="entry name" value="Cytosol_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049">
        <property type="entry name" value="ACYL COENZYME A THIOESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049:SF24">
        <property type="entry name" value="CYTOSOLIC ACYL COENZYME A THIOESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017331350" description="palmitoyl-CoA hydrolase" evidence="9">
        <location>
            <begin position="21"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="HotDog ACOT-type" evidence="10">
        <location>
            <begin position="27"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="HotDog ACOT-type" evidence="10">
        <location>
            <begin position="201"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR640170-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="357" mass="40011" checksum="E9255BC45C690BD7" modified="2018-12-05" version="1">MPTIVVGFFILLQNYLLMQTYYHQIQTLSLLLCCRVMLPDDANIMGNVHGGIILKMIEEAGSIVGTRHCNAQNGDRCLAALVRVEKTEFLSPVFIGEVVHVSAEITYTSKHSLEVQVKVIAENILTGAKKLANKAALWYVPCSLQNVDKITEVPPIEYASAEKEEEGRKRYEAQKIDRMETKARGEEVMPPRPNPERHTVGFSQSSLIHLVGPSDCTVVDFVYGGVAMKLMDEVAGIVAARHCNTNIVTASVDAINFHRKIRKGCVVTVTGRLTFVSNRSMEIEVLVDADSLMEKQIGKYRAVSAFFTFISLDKENKPLNVPPLKIEGDDEQKRFEEGKSRYLQNKARRLAEKERQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L3B4</accession>
    <name>A0A3B5L3B4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_027900770.1">
        <property type="nucleotide sequence ID" value="XM_028044969.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_027900771.1">
        <property type="nucleotide sequence ID" value="XM_028044970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L3B4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000002269"/>
    <dbReference type="Ensembl" id="ENSXCOT00000002303.1">
        <property type="protein sequence ID" value="ENSXCOP00000002269.1"/>
        <property type="gene ID" value="ENSXCOG00000001816.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114161603"/>
    <dbReference type="KEGG" id="xco:114161603"/>
    <dbReference type="CTD" id="208"/>
    <dbReference type="GeneTree" id="ENSGT00940000157189"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01241">
        <property type="entry name" value="PH_PKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05595">
        <property type="entry name" value="STKc_PKB_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000033">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000027">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000838">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034677">
        <property type="entry name" value="Akt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039026">
        <property type="entry name" value="PH_PKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="5"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="153"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="411"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="455"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="455"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="470"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000002269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="482" mass="55708" checksum="6BA991B679D6F769" modified="2018-12-05" version="1">MDEVTVVKEGWLHKRGEYIKTWRPRYFILKSDGSFIGYKDKPEVSSDLSLPPLNNFSVAECQLMKTERPKPNTFVIRCLQWTTVIERTFHVETNSEREEWMQVIQTVANSLKSQQQDEEPMEIKFGSPSDSSGAEEMEIAMCKSRSKVTMSDFDYLKLLGKGTFGKVILVKEKATGMYYAMKILRKEVIIAKDEVAHTVTESRVLQNTRHPFLTTLKYAFQTHDRLCFVMEYANGGELFFHLSRDRIFTEDRARFYGAEIVSALEYLHSRNVVYRDLKLENLMLDKDGHIKITDFGLCKEGITDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHERLFELILMEEIRFPKTLAPEGKALLAGLLKKDPKQRLGGGPDDAKDVMTHKFFALINWQDVIEKKLIPPFKPQVTSETDTRYFDDEFTAQSITITPPDKYDSLDVEDSDQRTHFPQFSYSASIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L3R5</accession>
    <name>A0A3B5L3R5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome activator complex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Activator of multicatalytic protease subunit 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Proteasome activator 28 subunit gamma</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PA28 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L3R5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005020"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005080.1">
        <property type="protein sequence ID" value="ENSXCOP00000005020.1"/>
        <property type="gene ID" value="ENSXCOG00000003936.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183098"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008537">
        <property type="term" value="C:proteasome activator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000045">
        <property type="term" value="P:regulation of G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061136">
        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.180:FF:000001">
        <property type="entry name" value="Proteasome activator complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.120:FF:000001">
        <property type="entry name" value="Proteasome activator complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.180">
        <property type="entry name" value="Proteasome activator pa28, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.120">
        <property type="entry name" value="Proteasome activator pa28, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003186">
        <property type="entry name" value="PA28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036997">
        <property type="entry name" value="PA28_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036996">
        <property type="entry name" value="PA28_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009077">
        <property type="entry name" value="Proteasome_activ_PA28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003185">
        <property type="entry name" value="Proteasome_activ_PA28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036252">
        <property type="entry name" value="Proteasome_activ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10660:SF4">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10660">
        <property type="entry name" value="PROTEASOME REGULATOR PA28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02252">
        <property type="entry name" value="PA28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02251">
        <property type="entry name" value="PA28_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47216">
        <property type="entry name" value="Proteasome activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome activator PA28 N-terminal" evidence="7">
        <location>
            <begin position="14"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator PA28 C-terminal" evidence="8">
        <location>
            <begin position="110"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="255" mass="29361" checksum="B88A6914F1061E52" modified="2018-12-05" version="1">MLRGFNTFGIIYIHVDAFRERITGEAEDLVANFFPKKLLELDHFLKDPSINISELKEIHSEINLTVPDPILISNLHDGLEGQNAKKRKLEDGDEDAMGEFCVFVMPGGMMKCNGNLVELIEKVKPEIRTLIEKCNTVKMWVQLLIPRIEDGNNFGVSIQEETVAELRTVEGEAASYLDQISRYYITRAKLVSKIAKYPHVEDYRRTVTEIDEKEYISLKIIVSELRNQYVTLHDMILKNIEKIKRPRSSNTDALY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L5J3</accession>
    <name>A0A3B5L5J3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">[histone H3]-lysine(4) N-trimethyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.354</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60260"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.354"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.354" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L5J3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006082"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006152.1">
        <property type="protein sequence ID" value="ENSXCOP00000006082.1"/>
        <property type="gene ID" value="ENSXCOG00000004706.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000166832"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140999">
        <property type="term" value="F:histone H3K4 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.270.10:FF:000013">
        <property type="entry name" value="Histone-lysine N-methyltransferase SMYD1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050869">
        <property type="entry name" value="H3K4_H4K5_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12197:SF290">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE SMYD2-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SET" evidence="10">
        <location>
            <begin position="6"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="MYND-type" evidence="11">
        <location>
            <begin position="51"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="431" mass="49275" checksum="1FB0EE061B310B8A" modified="2018-12-05" version="1">MTGLIEGIEKFDSPGKGRGLRVTKAFKVGELLFSSPPYSYVLSVEERGNYCEFCFTRKEGLARCGKCKKAFYCNAKCQKGDWAMHKLECSAMNAFGENWCPSDTTRLVARILAKKKMMKERCVSEKMLLIEELQSHLEDEDNEKKERTDTDIAALHRFYPKHLELPDHKDLLTLYSQVACNGFTIEDDELSHIGTAVYPDVALINHSCLPSVIVTYNGTSAEVRAVQDMKPGDEVLISYIDLLYPTDDRNNRLRECYYFTCDCLECKTKSKDKAKLKICKQRDPVEPHVVDNMIRYARNSVREFRALKLAPPSELLDMCEQSLEKMGAVFDDSNVYMLHMIYQAMGVCIYMQDADGAIRYGEKILKPYSKLYPPYSLNVSSMYLKMGRIYINMDRNSEGTSALKKAMAIMEVAHGKNHPFLKDLRKEIGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7F2</accession>
    <name>A0A3B5L7F2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Adenosylhomocysteinase-like 1</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L7F2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008233"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008331.1">
        <property type="protein sequence ID" value="ENSXCOP00000008233.1"/>
        <property type="gene ID" value="ENSXCOG00000006229.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182981"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000002">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1480:FF:000007">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000024">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000056">
        <property type="entry name" value="Protein CBG18239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF3">
        <property type="entry name" value="S-ADENOSYLHOMOCYSTEINE HYDROLASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="75"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="492"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="492"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="514" mass="56613" checksum="C0AD7A6BC7A8BCDF" modified="2018-12-05" version="1">MTDSAVDSKTEVKQATKYVKETENVAEKYSALTVSKNSSDVNMNVGEMPSAAFTAVPTLKSVKKIQFANAEDKQEFSKFPTKAGRRSLSRSISQSSTDSYSSAASYTDSSDDETSPRDKAQVNSHGSTDFCVKNIKQAEFGRREIEIAEQDMSALISLRKRAQGEKPLAGAKIVGCTHITAQTAVLIETLVALGAQCRWTACNIYSTQNEVAAALAETGVPVFAWKGESEDDFWWCIDRCVNRDGWQANMILDDGGDLTHWVYKKYPSVFKKVRGIVEESVTGVHRLYQLSKAGKLCVPAMNVNDSVTKQKFDNLYCCRESILDGAMTKDNNLLGKFELSGLPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKDEIEKMVQDADKYKAEDDQQREKIAAKNSLESYAYHMKSSVEDDALKGKISEEEKKLVIDKCNQTISWLENNQMAEKKEYEHQQNELERVCKPVVAKLYQGAAAGSSSSQAGGTSQGPTVEEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L7H0</accession>
    <name>A0A3B5L7H0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5L7H0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006385"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006465.1">
        <property type="protein sequence ID" value="ENSXCOP00000006385.1"/>
        <property type="gene ID" value="ENSXCOG00000004940.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156306"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033192">
        <property type="term" value="F:calmodulin-dependent protein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097720">
        <property type="term" value="P:calcineurin-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043360">
        <property type="entry name" value="PP2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45673">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2B CATALYTIC SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="3">
        <location>
            <begin position="150"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="223" mass="25244" checksum="ABC0591558B9EDB8" modified="2025-10-08" version="2">MEMSENKPNDEPKLSTTDRVVKSVPFPPSHRLTAKEVFDSDGKPKVDVLKAHLTKEGRVDESVALRLIGEGAAILRSEKNLLDIEAPVTVCGDIHGQFFDLMKLFEVGGSPASTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEITNLDDIRKVSPEVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L8X3</accession>
    <name>A0A3B5L8X3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Plakophilin 4</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L8X3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006998.1">
        <property type="protein sequence ID" value="ENSXCOP00000006910.1"/>
        <property type="gene ID" value="ENSXCOG00000005337.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000178373"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="8859" checksum="04CEA4F768720F47" modified="2018-12-05" version="1">MPVPDQPAEQEKGAMVAPVMPASNGDRPETETTSSILASVKEQELQFERLTRELEAERQIVATQLERCKLGSDAGSMSSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L939</accession>
    <name>A0A3B5L939_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear protein 1b</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NUPR family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027887625.1">
        <property type="nucleotide sequence ID" value="XM_028031824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5L939"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007327"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007421.1">
        <property type="protein sequence ID" value="ENSXCOP00000007327.1"/>
        <property type="gene ID" value="ENSXCOG00000005666.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114153340"/>
    <dbReference type="KEGG" id="xco:114153340"/>
    <dbReference type="GeneTree" id="ENSGT00940000167280"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045786">
        <property type="term" value="P:negative regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018792">
        <property type="entry name" value="NUPR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17149">
        <property type="entry name" value="NUCLEAR PROTEIN 1 AND 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17149:SF3">
        <property type="entry name" value="NUCLEAR PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10195">
        <property type="entry name" value="Phospho_p8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="76" mass="9018" checksum="40B610240525C226" modified="2018-12-05" version="1">MATDMERLASFEEAYYDQYDYYNLHEYSCHAGGKGRTKREIELNTNRHCPAGHERKIAEKYHNSQMKRRRTKSASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5L9G6</accession>
    <name>A0A3B5L9G6_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Tyrosine-protein kinase receptor TYRO3</fullName>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5L9G6"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007221.1">
        <property type="protein sequence ID" value="ENSXCOP00000007130.1"/>
        <property type="gene ID" value="ENSXCOG00000005505.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155879"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000986">
        <property type="entry name" value="Protein tyrosine kinase 2aa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000484">
        <property type="entry name" value="Tyrosine-protein kinase receptor TYRO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF279">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR TYRO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017248404" description="Tyrosine-protein kinase receptor TYRO3" evidence="18">
        <location>
            <begin position="21"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="3"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="118"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="188"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="198"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="21">
        <location>
            <begin position="297"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="624"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="648" mass="71537" checksum="BF566C0B35E37C6C" modified="2018-12-05" version="1">MKPLLWVVVVALRTLTGSCGSVLFTKHPSNQTVSQGNEVRLGCAVEGLTEPDIIWMKDGEKLYSTDQVFLILGEQHWETSHSVKSVQQQDAGQYWCVVESHGQMFASERAWITVEGVPHFIQEPQDVATFPSIPFSLSCAAVGPPEPVEVLWWLGGVQEGGPQQSPSVLHVPGQNLCLSENPSCVFGYLYVKVVLPAAPVDLQLLRITDNNVTLSWKPGFMGYSQLSTCVIQVVRVSGRRWNLPQQEVQVPPHIHTLTGLRSHSNYSLRVSCVNEVGASPFSPWLTFITPESVPSVAPRNLSFELSGQKLSLQWARLKEDELQGKLLAYKLQWTHGGEPQEPLLFKENSTPMLDAARFFNASIQVAACTAVGCGPWSPPVLVGRFNQGGIHLMNASSQANMSVCVADFGLSKKIYSGDYYRQGSVSKLPVKWIALESLADNVYTTQSDVWAFGVTMWEIMTRGQTPYPGVENSEIYEFLIKGERLKKPPDCREDIYEIMHSCWSPVPKCRPSFPQLVAQLEALHLALSPVTPLKESLLYVNLEEDNSETDREEGAVGVLGILGPETAVEGSSWSVPWQRRAEDEEKDWLMVGSGAALAIRGDYRYIVGPCGAIAEEAEEVGSVERRGDLANGPVDELRDEDDDVVINV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAL2</accession>
    <name>A0A3B5LAL2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA helicase</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="21 22">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs. Required for the restoration of heterochromatin organization after replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAL2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006776"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006859.1">
        <property type="protein sequence ID" value="ENSXCOP00000006776.1"/>
        <property type="gene ID" value="ENSXCOG00000005232.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00910000144252"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14279">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17998">
        <property type="entry name" value="DEXHc_SMARCAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000014">
        <property type="entry name" value="SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000639">
        <property type="entry name" value="SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CUE" evidence="18">
        <location>
            <begin position="124"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="379"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="724"/>
            <end position="884"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="184"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="227"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="893" mass="102779" checksum="5EA0EEA82FCB91B4" modified="2018-12-05" version="1">MSMFNLDRFRFEKKGANKDPGCVSKSPDSEKENEPVTNFWSEKMLEIHEKYIKYFKCRNGQNRNLCLKASDVNPFTSFEISSRFSSQLQMMARRESRMDPAVLRTVVIFNPVKDGNRIILFWEKQEAMVRKLQKRFPDQDKEELRMVLQEHNWDVEEALQVLQITNIHTVHSRITFLSFSRMQTSTSSTSKPSPSSSASQVLSSDARLAKKQAAERKKKARATEEQVSSDEEEDVVTSEFEDSDDELESINVKKDVKDEILAFFQDASIDELSLISGCSVKKAQRIVELRPFDSWESLWDVFHKGNGLSDDLMHGCKVVLKERKVILGLMSKCQTISTKMVQRVTEVMESGTGSTKQPSLLNSQLQLKPYQLIGLKWLMLLNEHKLSGILADEMGLGKTIQAIAFLAQLYENGIEGPHLITVPSSTLDNWVRELRLWCPAVEVLIYYGSVEERRYLKHDILNGEVHFNVIVTTYNLAIGNDSDRSLFRKLPIKYAIFDEGHMLKNMNTLRYRHLMAINAEHRLLLTGTPLQNNLLELMSLLNFIMPTMFSNSTMQLSKMFSMKSHEEQSRFERDRITRAKLIMKPFILRRVKNEVLKQLPAKEEKIEFCSMSEKQQVLYEALLKKLKSTTIGEKRELCNVMMQLRKMANHPLLHRQYYTTEKLQAMSQLMLKEPTHHDASAALIQEDMEVMSDFELHRLCQQYFSIGSFQLDTSLLLDSGKFLHLKELLASLKSQGDRVVLFSQFTMMLDIVEVLLKHLQHRYIRLDGSTPIADRIVLIDEYNTDPDIFVFLLSTRAGGLGINLTSANVVILHDIDYNPYNDKQAEDRCHRVGQTRTVQVIKLISKDSIEDCILQLGQKKLKLEQDMTAADQGKGLYVGTQNKTNVQHLPSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LAQ2</accession>
    <name>A0A3B5LAQ2_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Inositol polyphosphate-5-phosphatase D</fullName>
        </submittedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LAQ2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007912"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008010.1">
        <property type="protein sequence ID" value="ENSXCOP00000007912.1"/>
        <property type="gene ID" value="ENSXCOG00000006069.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156202"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045779">
        <property type="term" value="P:negative regulation of bone resorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045659">
        <property type="term" value="P:negative regulation of neutrophil differentiation"/>
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        <property type="term" value="P:positive regulation of B cell differentiation"/>
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        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
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        <property type="entry name" value="C2_SHIP1-2_2nd"/>
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        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
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        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 5-PHOSPHATASE 1"/>
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        <property type="entry name" value="SH2 DOMAIN-CONTAINING PROTEIN"/>
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        <property type="entry name" value="DNase I-like"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1/2-like first C2" evidence="4">
        <location>
            <begin position="248"/>
            <end position="327"/>
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    </feature>
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            <begin position="519"/>
            <end position="619"/>
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            <begin position="723"/>
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    <sequence length="843" mass="95566" checksum="F9F25484FB1EB2CD" modified="2018-12-05" version="1">MNSYFLYPLFLSTTALIRRRRIAIFYRTVFLFFFLLQASEGVPIRFFAMLPELVEAYYNPNMGLVTHLQYAVQREEEAEEEQESNSLPPQLPPRNFLADAKESETKTSEQATKSLSDTYLMRLQHIDLSALSEEHQMAVQEYFRTSICLDAEQVQNGNPNLPGLKKLITAVCKNLNCEISRVLPTLEIFHRALDQQLSPGIGPKQVGDSGQSISYRLEQLTKLLYSIEDKVWAAAHMLVTFRHRKSLIPIVSFEVKQELLGISTKMVLKVDVESGKLFFKKSKDGPEDKYFVHNKILQLVKSQKMHAKLIIVVETEKEKILRKEFVFDDTKKREGFCQLLQQMKNKHSEKPEPDMITVFVGTWNMGNAGPPPNIDSWFHCKGLGKTRDDTADHIPHDVYVIGTQEDPLGEREWSDTVKNALRKITNISFKQVGVTVTVQKYSHLFLMCTCFLINDNRIPKIDLLFSKGHITFMSLWTIKNGLSLRSDALVFVDSLGPNSAPQGGIQIMNCVAILLTKSKTRFFIEFHSSCLEKTVKTSEGDNTEQSDGSIKVWFGNKLTPIISDPEYLLEQHILICVKSTDCDESYGEGCVALRAAQFCYREFQITLTHQGEKTGTLTGGIQLRTSEFKPTEKLYDFIKIERDDAAPSKGKGGDKKTIQILIDWFLSARNEDEKLSEMLDNPLYGSMAKSQAKDQGHPQPDRLTPTIDGDPNRPPLLVPRNRSFTSSENRSQPQTPVGLHPPGQKKPVIPSRSDGGMAPHRPPLPTKSRPEPQTPKPRDYRDTSEFPSKQRVPTRPGPLPPQKDSKARTKSLRAQLNQHSLMHFVSYFSAFRTSQDGPSSELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBA1</accession>
    <name>A0A3B5LBA1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Collagen, type I, alpha 1b</fullName>
        </submittedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000005154"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005215.1">
        <property type="protein sequence ID" value="ENSXCOP00000005154.1"/>
        <property type="gene ID" value="ENSXCOG00000004019.1"/>
    </dbReference>
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        <property type="component" value="Unplaced"/>
    </dbReference>
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        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="C:extracellular region"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:extracellular matrix structural constituent"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Collagen alpha-1 type I chain"/>
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    </dbReference>
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        <property type="entry name" value="Complement Module, domain 1"/>
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    </dbReference>
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        <property type="entry name" value="Collagen"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Fib_collagen_C"/>
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        <property type="entry name" value="COLLAGEN ALPHA-1(XXIII) CHAIN-LIKE ISOFORM X2"/>
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    </dbReference>
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        <property type="entry name" value="SI:CH211-266K2.1"/>
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        <property type="match status" value="4"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="FnI-like domain"/>
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        <property type="entry name" value="NC1_FIB"/>
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        <property type="entry name" value="VWFC_1"/>
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        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0176">Collagen</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017425070" evidence="13">
        <location>
            <begin position="23"/>
            <end position="1429"/>
        </location>
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        <location>
            <begin position="30"/>
            <end position="88"/>
        </location>
    </feature>
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        <location>
            <begin position="1195"/>
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        </location>
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        <location>
            <begin position="95"/>
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        </location>
    </feature>
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            <begin position="162"/>
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        </location>
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        <location>
            <begin position="194"/>
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        </location>
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            <begin position="302"/>
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            <begin position="450"/>
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        </location>
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            <begin position="830"/>
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            <begin position="1098"/>
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            <begin position="1144"/>
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            <dbReference type="ARBA" id="ARBA00004613"/>
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            <dbReference type="ARBA" id="ARBA00022525"/>
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            <dbReference type="ARBA" id="ARBA00022530"/>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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            <dbReference type="ARBA" id="ARBA00023278"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="SAM" id="SignalP"/>
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        </source>
    </evidence>
    <sequence length="1429" mass="135198" checksum="F269D552960E336E" modified="2018-12-05" version="1">MFSFVDIRLALLLSATVLLARGQGEDDQFGSCTSEGQSYNDKDVWKPEPCQICVCDMGTILCDEVICDDTSHCEDPYIPDGECCPVCPDEGLPGLPGNDGIPGTPGLPGPPGLPGIPGLGGNFSPQMSYFDPSKSSGPPVPGPIGPPGPRGAPGLPGPPFQGPSGSPGPQGEPGEPGAVGPMGPRGPAGPPGKNGDDGEAGKAGRPGERGAAGPQGARGFPGTPGLPGIKGHRGFPGLDGAKGDGGPAGPKGEAGVPGENGIPGAMGARGLPGERGRPGPAGPAGARGNDGNTGPAGPPGPTGSAGPPGFPGAVGAKGETGPAGGRGSEGPQGARGEPGNPGPAGPAGPAGSPGSDGQPGAKGANGAPGIAGAPGFPGNRGPAGAQGGAGAPGPKGNNQGEPGPPGPKGEAGAKGEPPAGLQGLSGPSGEEGKRGPRGEPGGAGPRGPPGERQGAPGARGFPGSDGAAGGKGAPGERGAPGPMGSQGATGEGGSSGAPGAPGSKGVTGSPGSPGPDGKVGPAGAPGQDGRPGPPGPSGSRGQPGVMGFPGPKGAAGESGKPGERGPGGAGGSVGAPGKDGDVGAPGPSGPAGPAGEKGEQGPSGAPGFQGLPGPQGATGESGKPGEQGVPGEAGPPGPSGPRGDRGFPGERGGPGPAGPTGSRGSPGAGGSDGPKQGEPGAGGAPGSAGLPGMQGMPGERGSAGIPGPKGERGDAGAKGSDGAAGKDGVRGLTGAIGLPGPPGSQGEKGESGPSGVVGPSGPRGSPGERGETGPAGPAGFAGPPGADGQPGAKGESGDTGPKGDAGPAGPPGPVGSSGPQGPAGATGPKGARGGAGPPGSTGFPGPAGRVGPPGPSGAAGPPGPSGPVGKDGPRGSRGETGPAGRPGESGAAGAPGPSGEKGSPGSDGSPGAPGLPGPQGIAGVRGIVGLTGQRGERGSSGIPGPAGEPGKQGPSGLVGERGPPGPAGPPGLSGAPGEAGREGSPGHDGASGRDGPPGPKGDRGESGMAGPPGPPGAPGAPGGVGPSGKSGERGESGPAGPVGPAGIGGPRGSSGPAGAKGDRGEAGEAGDRGHKGHRGLSGQPGLPGPPGVHGDRGPAGASGPAGPRGPSGNNGSPGKDGQNGTPGPIGPPGPRGRNGELGPSGPPGLPGPPGPPGAPGSAFEFVAPPQQEKGPDPLRGGYRADDPNMMRDRDMEVDTTLKTLTQKVENIRSPDGSQKSPARMCRDLRMCHPEWKSTYWVDPNQGSSLDAIQVHCNMETGETCVYPIDSNIRMKNWYLSKNLREKKHIWFSESMTGGFQFQYGTEGADPEDVNIQISFMRLMSNQASQNVTYHCKNSIAYMDSAAGNLKKALLLQGSNDVEIRAEGNSRFTYSVSEDGCSSHTGTWGKTVIDYKTSKTSRLPIIDIAPMDVGAPDQEFGVEVGPVCFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBL9</accession>
    <name>A0A3B5LBL9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zinc finger CCHC-type containing 24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ZCCHC24</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBL9"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008008.1">
        <property type="protein sequence ID" value="ENSXCOP00000007910.1"/>
        <property type="gene ID" value="ENSXCOG00000006073.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008264"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027377">
        <property type="entry name" value="ZAR1/RTP1-5-like_Znf-3CxxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057809">
        <property type="entry name" value="ZCCHC24_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033446">
        <property type="entry name" value="ZCCHC24_Znf-3CxxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025829">
        <property type="entry name" value="Zn_knuckle_CX2CX3GHX4C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23490">
        <property type="entry name" value="ZCCHC24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13696">
        <property type="entry name" value="zf-CCHC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17180">
        <property type="entry name" value="Zn_ribbon_3CxxC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01328">
        <property type="entry name" value="zf-3CxxC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="5">
        <location>
            <begin position="119"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="226" mass="25449" checksum="72995F89E43C80DA" modified="2018-12-05" version="1">MMSAIETGLYQPAQLLSWVYMSLQDAPGSAVDGVKVESDSSARDGTERIAADLSSNYLSNFFHLNTEALTKSFYKGSSPYGSFSNIVDGLNSLADHFSDLSLSPETHKPSKRPPPNYLCHLCFNKGHYIKDCPQARPKGEGLTPYQGKKRCFGEYKCPKCKRKWMSGNSWANMGQECIKCHINVYPHKQRPLEKPDGLDVSDQSKEHPQHLCEKCKVLGYYCRRVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LBM2</accession>
    <name>A0A3B5LBM2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 2 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LBM2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009588"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009700.1">
        <property type="protein sequence ID" value="ENSXCOP00000009588.1"/>
        <property type="gene ID" value="ENSXCOG00000007301.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160428"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050848">
        <property type="entry name" value="Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24333">
        <property type="entry name" value="HOMEO BOX HB9 LIKE A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24333:SF5">
        <property type="entry name" value="VENT HOMEOBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0371">Homeobox</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="5">
        <location>
            <begin position="131"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="24"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="185" mass="20554" checksum="BCACC6447AEDC7A5" modified="2018-12-05" version="1">MDLPASGRSRFLIDSLLALRPPAALLSRPNPPELPGGVPEPPSPQREPVPRRESPALPGPAHRNRCLGASFLIRDILADCRFCSDPGHPEPGAEPIRPGPAENSFDEESSVLQFSDRFRSAHAGPEPGLLKKVRKARTAFSEQQLTQLERSFQNRKYLSVQDRMALAASLQLSDTQVKTWYQNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LCC4</accession>
    <name>A0A3B5LCC4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LCC4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000005524"/>
    <dbReference type="Ensembl" id="ENSXCOT00000005590.1">
        <property type="protein sequence ID" value="ENSXCOP00000005524.1"/>
        <property type="gene ID" value="ENSXCOG00000004316.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01110000271458"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030414">
        <property type="term" value="F:peptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.75.10">
        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036645">
        <property type="entry name" value="Elafin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008197">
        <property type="entry name" value="WAP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00095">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00003">
        <property type="entry name" value="4DISULPHCORE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00217">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57256">
        <property type="entry name" value="Elafin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51390">
        <property type="entry name" value="WAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WAP" evidence="1">
        <location>
            <begin position="55"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000005524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="99" mass="11020" checksum="66170EF20A7CE898" modified="2018-12-05" version="1">IGLSWSQVCKKTVKKMFICLKPGHCPKSFMTNGKCCTVNQKCCFNGCDYICTPSVPEKPGSCPYKQFTPWCYNFCDHDGDCPGAKKFCPTSCGRACRQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDE3</accession>
    <name>A0A3B5LDE3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Scaffolding anchor of CK1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM83 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDE3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000008681.1">
        <property type="protein sequence ID" value="ENSXCOP00000008575.1"/>
        <property type="gene ID" value="ENSXCOG00000006565.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164021"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050944">
        <property type="entry name" value="FAM83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012461">
        <property type="entry name" value="SACK1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16181:SF14">
        <property type="entry name" value="FAMILY WITH SEQUENCE SIMILARITY 83 MEMBER FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16181">
        <property type="entry name" value="PROTEIN FAM83A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07894">
        <property type="entry name" value="SACK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Scaffolding anchor of CK1" evidence="3">
        <location>
            <begin position="15"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="70"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="93"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="174" mass="19457" checksum="58C0000DFBEC4F33" modified="2018-12-05" version="1">MAESQLLCLDNQHVNEKVPESRPEFYYSEEQRAALEQLLRHGDGAFKMRLQEDNIRDFLCAREVRAIRDSFQEYDPDSEPEPGEPDKPKEATSADSGVHSTYWPQMSDTEVPALDIGWPACSGVYKGVTRVSAYTHPPKEPGPHIKEVVRRLIQGAQKVTGRRAGGSCDSVTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LDM2</accession>
    <name>A0A3B5LDM2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL2m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L2, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LDM2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000008932"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009038.1">
        <property type="protein sequence ID" value="ENSXCOP00000008932.1"/>
        <property type="gene ID" value="ENSXCOG00000006806.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153244"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000025">
        <property type="entry name" value="39S ribosomal protein L2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000157">
        <property type="entry name" value="39S ribosomal protein L2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF73">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 RNA-binding" evidence="10">
        <location>
            <begin position="19"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL2 C-terminal" evidence="9">
        <location>
            <begin position="109"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00082723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000008932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="237" mass="26262" checksum="2C136F61E023ADA8" modified="2018-12-05" version="1">TQWELADFDVFVFLSDLLFTGRIRTRGIGGGHKQKYRWVDFQRLRYEPNKEGQPFEEKVVEVRYDPCRSADIALIAGGNRKRWIIATEKMQAGDIIKTSGVIGRMAVSANEGDAYPLGALPVGTLVNNLEVQPGKGSQYIRAAGTSGVLLRKVNGTAIVQLPSKQQVQVLETCIVTVGRVSNIDHNKRIIGKAGRNRWLGIRPSSGLWKRKGGWAGRKIKPLPPMKSFINLPLISTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LEE0</accession>
    <name>A0A3B5LEE0_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LEE0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000006234"/>
    <dbReference type="Ensembl" id="ENSXCOT00000006308.1">
        <property type="protein sequence ID" value="ENSXCOP00000006234.1"/>
        <property type="gene ID" value="ENSXCOG00000004824.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018691"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019185">
        <property type="term" value="C:snRNA-activating protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019188">
        <property type="entry name" value="SNAPC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15131">
        <property type="entry name" value="SMALL NUCLEAR RNA ACTIVATING COMPLEX, POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15131:SF3">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09808">
        <property type="entry name" value="SNAPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000006234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="241" mass="28396" checksum="016A8D9D909D44A7" modified="2018-12-05" version="1">MESYRKPVKSDCEALLSRFQQTQSVRFEVFSNIWKEMKFVHIFRQSHNKRAFCRLVLDVAYCYFMPPFSFQIRVGGLYLLYSFYHCQTTSPPEQIRVALKDWEDVRNFEKESLDAQHLDVAYILQQLMTCKAFLFTAMPTLLHYSRKRTEEKPELCDEFMERPCRPQELINTQLSEEMSNIHELYKNTKASISSQMSTAAAASVSLIRGGIVLELCSAVMEFYNWQQNRVSFPHLITFNPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LHI4</accession>
    <name>A0A3B5LHI4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NOP9 nucleolar protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LHI4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010317"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010440.1">
        <property type="protein sequence ID" value="ENSXCOP00000010317.1"/>
        <property type="gene ID" value="ENSXCOG00000007822.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004964"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040000">
        <property type="entry name" value="NOP9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22493">
        <property type="entry name" value="PUF_NOP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="89"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="468"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="591" mass="65679" checksum="0142B1B956C8C200" modified="2018-12-05" version="1">MLAKDGEKLQKGGVRKRRRPAEDGGRAEWKKKKNRGEEEKPGQKPRGDGGRKRLDALSVGYFRRVGERLSEGFEEDEEREMFVGNVLAEVKGKAGLVATDRTGSITLQRLLPLSSPDQVGEVLAELGGESGAGFKEVSCDRCGGHVVESALRQMSRWEEEEEESAGMLESQVLSLSQVVKDNGSEFIGHVHGSHVVRTLLHVLAGCVGPPQHLKAAPQLTDFEIPTSFWYELKSLTETLMENINCNAVFQTMLTVCHRKRPKLCKQLLKRTMEYLSSRSSAPGVSPLLVFLKDQASSRLIETIIQLSHKSLLRDLYKGHFKGSLVDLALHPIANFPVQRLTAASAEHKLFPKLFDELVQGVEAVLAAGHMGVIVQLAESCAESGEKQDDMMRCLLNSFHCAEPGSRHVCCLPLFMSLLTYEVYYQTDATEGSTQKEVPLTSICYHGSRLVQALARFKERSHLLSSLRTLSPADLLTMASDPSGSHVLQALITTSSDKGRGKILKRLEYVQMACSRLGSRVLEAAWNSAAVSQRQSIAQELVPSESKLRSDQFARHVWAKFGLSHFVHRRAHWQEIQTGESKKRKLFSDLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIB4</accession>
    <name>A0A3B5LIB4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the POU transcription factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIB4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000007609"/>
    <dbReference type="Ensembl" id="ENSXCOT00000007705.1">
        <property type="protein sequence ID" value="ENSXCOP00000007609.1"/>
        <property type="gene ID" value="ENSXCOG00000005858.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160880"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000007">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF43">
        <property type="entry name" value="POU DOMAIN, CLASS 4, TRANSCRIPTION FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="12">
        <location>
            <begin position="178"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="271"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="273"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000007609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="328" mass="36245" checksum="92459301D8DC1CF2" modified="2018-12-05" version="1">QNLAFSFCHVCMHQKLNFSSQNQDFIKLCHNRLQGNIFGGFDESLLARAEALAAADSIVSQGKSHPFKPDVTYHTMSSVPCTSASSSSSTTVPISHHHHHHHHLGQTLEAGDLLDHLSTSLAVTGMGAPEPPGMTATSHHHQHPHHHLQTMGQLHQAMATMAHPHSLSVHGGMACVSDVESDPRELEAFAERFKQRRIKLGVTQADVGAALANLKIPGVGSLSQSTICRFESLTLSHNNMIALKPVLQAWLEEAEAAHREKSSKPDLFNGSERKRKRTSIAAPEKRSLEAYFKIAAIAEKLDLKKNVVRVWFCNQRQKQKRMKYSAVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LIF7</accession>
    <name>A0A3B5LIF7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Prefoldin subunit 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits. Component of the PAQosome complex which is responsible for the biogenesis of several protein complexes and which consists of R2TP complex members RUVBL1, RUVBL2, RPAP3 and PIH1D1, URI complex members PFDN2, PFDN6, PDRG1, UXT and URI1 as well as ASDURF, POLR2E and DNAAF10/WDR92. Interacts with URI1; the interaction is phosphorylation-dependent and occurs in a growth-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LIF7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011873"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012009.1">
        <property type="protein sequence ID" value="ENSXCOP00000011873.1"/>
        <property type="gene ID" value="ENSXCOG00000008985.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009272"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23163">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000002">
        <property type="entry name" value="Prefoldin subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027235">
        <property type="entry name" value="PFD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13303">
        <property type="entry name" value="PREFOLDIN SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="120"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00066061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="151" mass="16303" checksum="7D33C44BA85B9D9A" modified="2018-12-05" version="1">IIAQQTGSCVAPPGKLSVVATFQRMRQEQRNMASKAAELEMDINEHSLVIDTLKEVDSSRKCFRLVGGVLVERTVKEVLPALENNKEQISKVIETINTQMQAKGRELTEYRERYNIRLVGEGEGEGQGQSAAPSGNSEGGGSKSGAGVLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LJ14</accession>
    <name>A0A3B5LJ14_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Calsyntenin-2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Postsynaptic cell membrane</location>
            <topology evidence="18">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the calsyntenin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LJ14"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010645"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010769.1">
        <property type="protein sequence ID" value="ENSXCOP00000010645.1"/>
        <property type="gene ID" value="ENSXCOG00000008046.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183086"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051965">
        <property type="term" value="P:positive regulation of synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050806">
        <property type="term" value="P:positive regulation of synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000025">
        <property type="entry name" value="Calsyntenin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000029">
        <property type="entry name" value="Calsyntenin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000062">
        <property type="entry name" value="Calsyntenin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045588">
        <property type="entry name" value="CLSTN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14139">
        <property type="entry name" value="CALSYNTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14139:SF6">
        <property type="entry name" value="CALSYNTENIN-2 ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19699">
        <property type="entry name" value="CLSTN_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 21" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="795"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="24">
        <location>
            <begin position="35"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="24">
        <location>
            <begin position="152"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="824"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="22">
        <location>
            <begin position="827"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="22">
        <location>
            <begin position="862"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="894"/>
            <end position="912"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00035006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00035015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00069677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="919" mass="102593" checksum="8E73923951F2BFA3" modified="2018-12-05" version="1">AILMDIINHSSILAQGLGSSALSEAISIILHKPWIETSYHGVITENMETVLLDPPLVALDKDAPVPYAGEICSFKIFGQDAPFEAVVLNRTSGEGVLRASSHVDCESQKEYTFIIQAYDCGDGPSGANWKKSHKAVIHIQVDDVNEFSPVFRESLYKASVTEGKIYDSILQVEAWDQDCSPQYSQICNYEIVTGGTPFAIDRNGNIRNTERLSYEKQQSYRIMVSAFDCGQKKAKEDVAVHIEVKPVCKPGWQGWNKRVDYEPGTGSKQLFPKMHLETCGGPLSGVKAMVELQTNHIGKGCDRETYSEKSLQKLCGAASGSTDLLPAPSPSTNWTASLLTDSGRESDLIYRFDGRQAANVPFHVVPQNLTDQFTIATWMKHGPSPGLRAEKETILCNSDKTEMNRHHYSLYVHNCRLVFLLRRDFTQIDTFRPAEFHWKLEQICDKEWHYYVINVEFPAVTLFVDGVTYEPYLVTDDWPIHSSKIDTQLTVGACWQGGEVATPRFTQYFHGSLSGLTIRPGRIETQKVISCLQVCKEGLDINSLESLAKDIKFHFNPAQSVLVVEGGDVDSINTAMIKVSYINSRQFPTPGLRRLHISTSVQCFGEDTCVSIPDIKAVVMVLPPSEPRITISGSKQLVRPASDLRSPLGVAPFKELRITSTVMKGDNRRPGVMEVMHNLDYCDVLVIGDELEPENESLEIQHSALLGKHLDATNSTSGISIYGVDSMAHYEQVLRQLRYRNWRPASLTERRFRLTCSELNGRYTSNEFNLEAGFGLFINSFFFLFSVHYTVSPPAATVVIVVCIAALVIIVVIGIYRIHATHQEGSRDEEDEIKDPESDWEGSGLNITVNPMEVTETVSREECEEEEDEEDDAELVGGMTIVESDSSDEDEEEGNKGREEVNGRKQKGKPEWDSSCGTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LKD5</accession>
    <name>A0A3B5LKD5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Hexosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Golgi apparatus membrane</location>
            <topology evidence="1 13">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LKD5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011115"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011242.1">
        <property type="protein sequence ID" value="ENSXCOP00000011115.1"/>
        <property type="gene ID" value="ENSXCOG00000008399.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163421"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008499">
        <property type="term" value="F:N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000001">
        <property type="entry name" value="Hexosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002659">
        <property type="entry name" value="Glyco_trans_31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214">
        <property type="entry name" value="BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11214:SF115">
        <property type="entry name" value="HEXOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01762">
        <property type="entry name" value="Galactosyl_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="328" mass="37101" checksum="CC6EC1F23C8BDF28" modified="2018-12-05" version="1">MLKDPYIKDILICVLVGVTVVIIYLTSEYKPDHLYGPANKKSNATASSLPEPKWEDPGPYHVAYPRKYRFVMDNSPTCKTATPFLILMVPVAPANLEGRDTIRRTWGGKKMVLGRLVETVFIVGLPGGVDANQQQARLKQESERYRDLIQSNFQDSYRNLTIKTMVMLEWLAQHCAKASFMIKIDSDMLLHVDNLVALLLDPETPKENYMSGLVWWHSPVSRNPFNKFYMPSHVIAEPVYPPYPLGMAYVMSLDLPKKILSVSPHIKPINIEDAYLGLCLNRLGVSPTNPPDQRMFQVIPRHPLSRCNLSKVIATTTTSIKTHLVQSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LL74</accession>
    <name>A0A3B5LL74_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LL74"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000010316"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010439.1">
        <property type="protein sequence ID" value="ENSXCOP00000010316.1"/>
        <property type="gene ID" value="ENSXCOG00000007821.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286924"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033691">
        <property type="term" value="F:sialic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd20987">
        <property type="entry name" value="IgC2_CD33_d2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05712">
        <property type="entry name" value="IgV_CD33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000032">
        <property type="entry name" value="palladin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051036">
        <property type="entry name" value="SIGLEC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12035:SF107">
        <property type="entry name" value="MYELIN-ASSOCIATED GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12035">
        <property type="entry name" value="SIALIC ACID BINDING IMMUNOGLOBULIN-LIKE LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0430">Lectin</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017243129" description="Ig-like domain-containing protein" evidence="12">
        <location>
            <begin position="19"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="515"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="140"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="239"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="325"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="610"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="610"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="630" mass="70033" checksum="3963C954738860B0" modified="2018-12-05" version="1">MWCLELLIPLLLIINANCQWNIWLPRDISAMTNSCVVIPCSFMYPSGIRPARGVHGIWYFGQPYPQLFPPVVFKSLTDVVHESYKGRTKLLGDLNQRNCTLLIGNVGTEHSGRYYFRADIGGANKYSYPDYAELKVLDQPNIDIPEEIVSDESLELTCYAPDNCPDMTPEIQWMYTDYLPDPVFSAEYTEESNTAVISNTLTFTPRPMHNGQLLGCRVYFPNTTLVYERLISLDVKYSPRSVWVNVSSEVMEGSSVTLHCEVDSNPSPRVAWLFEGQEVLWDTASNISISLDDVTPENEGIYTCVGDNGYGMMNTSLYLAVKYPPREPVVNDSMTVVQGSSLALHCNTMGSPAPTLTWLKDGELAGSITAGELSVLEIMDITPQQTGQYRCLAENEHGRTSASLNITVEYAPVFLEESKCTVVREGVQCVCVAVGNPEPTIEFYLPDLNITINETDGRFNFYTHTDGHTSTAMIKLREKGERADNNGPAVNVHCSMYNIYGRESVLLELQQEKKYMMAVIVGTIGGVAVIAFIIAAVRYVGQNNKKENGNPRQDVVLENPALYYSAVKKDKQNLRKKVGEDGDYQPVGSVAGLERQELNYASLEFIGARPREGASGRGDDGSNYTEIKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LLP3</accession>
    <name>A0A3B5LLP3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleoprotein TPR</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPR family.</text>
    </comment>
    <dbReference type="STRING" id="32473.ENSXCOP00000011625"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011757.1">
        <property type="protein sequence ID" value="ENSXCOP00000011625.1"/>
        <property type="gene ID" value="ENSXCOG00000008770.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111014"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901673">
        <property type="term" value="P:regulation of mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057974">
        <property type="entry name" value="NUA/TPR/MLP1-2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012929">
        <property type="entry name" value="Nucleoprot-TPR/MLP1-2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057577">
        <property type="entry name" value="Nucleoprot-TPR/MLP1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18898:SF4">
        <property type="entry name" value="NUCLEOPROTEIN TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18898">
        <property type="entry name" value="NUCLEOPROTEIN TPR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25481">
        <property type="entry name" value="Nucleoprot-TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25785">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07926">
        <property type="entry name" value="TPR_MLP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoprotein TPR/MPL1" evidence="9">
        <location>
            <begin position="159"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="NUA/TPR/MLP1-2-like" evidence="10">
        <location>
            <begin position="462"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoprotein TPR/MLP1-2" evidence="8">
        <location>
            <begin position="1014"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="617"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="987"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1576"/>
            <end position="1688"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1752"/>
            <end position="1834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1875"/>
            <end position="2045"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="2196"/>
            <end position="2283"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="98"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="418"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="534"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1036"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1204"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="628"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="987"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1579"/>
            <end position="1603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1611"/>
            <end position="1622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1778"/>
            <end position="1797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1893"/>
            <end position="1905"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1914"/>
            <end position="1967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1975"/>
            <end position="1996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="2000"/>
            <end position="2015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="2196"/>
            <end position="2216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="2268"/>
            <end position="2277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="2299" mass="259928" checksum="89798C69ACD56846" modified="2018-12-05" version="1">MAALLLQELDSSEISKLPTTLQSKFEKLLLDRQYEIDSLKALHEQFRVDSEQQFFEKVDQLQRCRAELLSQAQAHLKVKEDFTKLGKTSFALLLFSYLTLLLKAKEELEGEKRELVRTLERRSLEVENLNDDLRQLNDKVMEVNTSKMAMQVKLDEHEAAEVNIKYKEKRMEQEKELLHGQISWLKDELKAKTEGLLTLSRKKGNEILELKCKLENKEDELTKLQDQVTSLKTSNDHLQKQSEEMVNKLKEAKEQQANSEEKFRNELNANIKLSNLYKGAAADSEAKSEELSRAVEELHKLLKDAGEANKALEEKLQEMNDAADKTVNELKEKIQSLEKELDNANDLLSTSKLRGPAGASVQAEDQITTMSPTAAAVSKIKPGMKLTELYTAYLESQEQLQLERLENKRVNKYLDDIVQEVEAKAPILKRQRDEHERMQKSVASLSAKLEQAVKEVHRLQNEADEANKRSTLLERDSQRYELQVADMAQQVRVLLIELEEARGNHVLQEEEVSSADISSTSEVISQHLVTFRSVEELQKQNQRLLVALRDLSDAQEKEEQESTGNKRVELEQSLEKAQAELESLKEQRSQQIKMTESIVRQRDMYRVLLAQATGVAFPQQGTPPEEFSLTSNPRRSPATTPTALVTMAESAEALEAKAALRQLQEVFSAYKKERLDSDKAMNEQNEKLQEQLSDLRSQNAKISTQLEFASKRYEMLQDNVEGYRKEIASLREKEQKMAATTQKLEQTVHTLNEDLKATKEKLTLTEGQAEKLRKERDMLKLVESRLNQEKETFVTQQQSQNLLLTNLQAIQATLERAEADTRQRLNSQIEKQELQISQLQKRLEQEVEQRHLCSRTQEVQLIDVKRQLETQVALHQKTKELLNAAELELNTLRMQQGRTEARHTLGLQGSDQEREELQGRLQLAETRAEELAESLRAATSSMEQYRAMAQSLEESLETEKQVTEQARLSVEACLKEAQERHRTLEEKLQEAEKEKQDLEESKKKDLASLEEQRSLSGAQTDHQAVLQQLAIAETQKQQALQDRQEQAKLAAEAQDKYEREMMLHAADVEALQAAKAQALQAGELRNQLEEKVQRVGAELLEARVSWEVQEKILKEETSKMESRYEELQRQNTLLHEQVQSMSTNMAANIQRATNESSLNVSLSEEGKSQDQLLEILRFVRREKEIAESRFEMAQGESLRHRLRVEHLERELREVRDSLTAARERMQVTAKTLAQHDELMKKTETMNILVETNKMLREEKTKMEQELQQTKAKVQKMESDVFPLQQSNSELSEKNGMLQAEKAILEEDIKRWKARTQNLLSQQKDSDPDEYKRLNTEKELHLKRIQQLTEENSRLKAELNKINNLTTSLQGQMQNMRESMSKANDDRNVLRKDLETKNQEIQEKVRTITQVKKIGRRYKMQYDELKVQHDKLVADAAAGPSQDEEAHQASAQELQSVKDSLNQAETRTKELEGQLENINKVVTERETELRNVQEQSSRLQTELNRLRQELQEKTTQEESLRQQMAEKDEKTRKALLVARQKISHVTATKEQLQRENEELKQQREELEVRVSALRSQYEGRLSRQERELRDLRGQQERQEQRDEPPEAGPSKTQEQQRTTEQRQISLKTTPAADRGSTSASEPPTANIKPTPVVATASKQPVNPGNKPTPRASIRPMITPVPPPTPTATVMPTTQVETQEAMQSTEAPPVEHVTVYGSASGSVRSASPNVQTTLAGSMLTVQQTQVQATAFVQPTQQQSVTHPEPDNREPSPVTIEATPSSQVEWPSTSSSSSSSVFVSATPGTSALSKRPREEEESSAAAFTEVEATQEEPRAPMAKKLRIIQRVGPEVRRKRAEEEGFNLTKWLSGRFMMFSNLEEAEEDSASQSVPAEPAVTPSQSETPDQLQQEVIVILTDSESEEEEEEEGEEQDYAEEEEEDEEEEEEEEEEEDEDEEDEDDGGMEEEGEESNEGSGDGNEVYEGDDTEGADVTDPGTETEESLGASDSTQRPADSQTPSFEGSPMEALSAEQPITSSGARIPQSPRRPVQQLLPQLNIHPAQSSELGPSAQVHTLQRIRRQSVGRLNPGMPSSAQHFFEEDDRMVPSIPTLVPPRSEGFDQAIHSPQVAGVPRFRFDLSEDVPQTSSSSHSDLGQLASQGGLGLYESPLFLATHEEESGGRSVPTTPLQVAAPVTVFSEAAQSDATEHASQSVPMVSTSTPGLVLPGTAGGGEERDIFLEPDTESAEASVDAVVSQGDAEEPSQPSDKANLPSTSQEPSSSSAGNALKLIHNGRLGYQVRPMRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LN06</accession>
    <name>A0A3B5LN06_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LN06"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012100"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012238.1">
        <property type="protein sequence ID" value="ENSXCOP00000012100.1"/>
        <property type="gene ID" value="ENSXCOG00000009143.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156345"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051496">
        <property type="term" value="P:positive regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000083">
        <property type="entry name" value="LIM domain kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000038">
        <property type="entry name" value="LIM domain kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000197">
        <property type="entry name" value="LIM domain kinase 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050940">
        <property type="entry name" value="Actin_reg-Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485">
        <property type="entry name" value="LIM DOMAIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46485:SF7">
        <property type="entry name" value="LIM DOMAIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="14 19" id="KW-0440">LIM domain</keyword>
    <keyword evidence="8 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 19" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="22">
        <location>
            <begin position="47"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="23">
        <location>
            <begin position="125"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="286"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="611" mass="69602" checksum="0730AC4841FFFD28" modified="2018-12-05" version="1">TLTTNHPQRREHMHRKCYDCECILSNWYYERGGQLYCKKHYWSRFGEHCHGCKETVSTGLVMVAGEQKYHPECFTCMSCEMVIGDGDTYMLVERSRLYCGHCFCQGVVPAVRSTSLTQRPHMVALVFLPPTAAGCRGLTISADFSQDKKLLITVTNLMNYCTTHSPPDLLSSVQAGDQVLEVNGIPVHNISPDEINCVIHDTSRPLQSYLHVPLGHMKLHGPPGLEFDTCNLSCFLFSRRSYSIDKYPLSPGALSFLSQKREMVRSESLRVDPGERTHRIFRPSDLIHGEVLGKGCFGQAVKVTHQETGEVMVMKELIRFDEETQKTFLKEVKVMRCLDHPNVLKFIGLFYKDKRIHFVSEYIQGGTLRETIAKMEDFPWNLRVGYAKDIAAGMAYLHSMNVIHRDLNSYNCLVREDQSVVVADFGLARLVMDERSRTKTSSLERSVKGPLSELRKPDRRKRYTVVGNPYWMAPEMIHGKTYDERVDIFSFGIMTCEIIGRVSADPDYLPRTNDFGLNVESFLQQYHSPLCPSVFLPLAVLCCDIDADKRPSFSKLEEWLDNLLMNLEISLPLLSELEQLCQSSGQGICPHVPLFLQIQTKPSGVIIYILW</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LNS7</accession>
    <name>A0A3B5LNS7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">[histone H3]-trimethyl-L-lysine(9) demethylase</fullName>
            <ecNumber evidence="3">1.14.11.66</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(9)-[histone H3] + 2 2-oxoglutarate + 2 O2 = N(6)-methyl-L-lysyl(9)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:60200"/>
            <dbReference type="Rhea" id="RHEA-COMP:15538"/>
            <dbReference type="Rhea" id="RHEA-COMP:15542"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the JHDM3 histone demethylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.66" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LNS7"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000009499"/>
    <dbReference type="Ensembl" id="ENSXCOT00000009611.1">
        <property type="protein sequence ID" value="ENSXCOP00000009499.1"/>
        <property type="gene ID" value="ENSXCOG00000007232.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154930"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051864">
        <property type="term" value="F:histone H3K36 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140684">
        <property type="term" value="F:histone H3K9me2/H3K9me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048514">
        <property type="term" value="P:blood vessel morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000048">
        <property type="entry name" value="Lysine-specific demethylase 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF119">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="JmjN" evidence="15">
        <location>
            <begin position="15"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="16">
        <location>
            <begin position="140"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="351"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000009499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="360" mass="41717" checksum="1138E6423821B50A" modified="2018-12-05" version="1">MASEALSQNHGSKVIMTFYPTAEEFKNFSRYIAYIESKGAHRAGLAKIVPPKEWRPRGSYEDIDDLVIPAPIQQVVTGQSGLFTQYNIQKRAMTVKEFRKIANSDKFCSPHYDDFEELERKYWKNLTFNPPLYGADVNGSLYDPEWNICHLGTILDTVERDSGITIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINYLHFGEPKSWYCVSPEHGKRLERLAKGFFPGSSQNCEAFLRHKMTLISPSILKKYGIPFEKITQEAGEFMITFPYAYHAGFNHGFNCAESTNFATERWIEYGKQAVLCSCRKDMVKISMDVFVRKYQPDRYEQWLAGRDTVPIDHSKPTPEGRQSWLSRQQSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LPZ8</accession>
    <name>A0A3B5LPZ8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="15 20">Histone deacetylase 8</fullName>
            <shortName evidence="20">HD8</shortName>
            <ecNumber evidence="5 20">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="25 26">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility. In addition to protein deacetylase activity, also has protein-lysine deacylase activity: acts as a protein decrotonylase by mediating decrotonylation ((2E)-butenoyl) of histones.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N(6)-(2E)-butenoyl-L-lysyl-[protein] + H2O = (2E)-2-butenoate + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:69172"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13707"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:35899"/>
            <dbReference type="ChEBI" id="CHEBI:137954"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:69173"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:58197"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(6)-acetyl-L-lysyl-[protein] + H2O = L-lysyl-[protein] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58108"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:58109"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 20">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19 20">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="5 20"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LPZ8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011496"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011628.1">
        <property type="protein sequence ID" value="ENSXCOP00000011496.1"/>
        <property type="gene ID" value="ENSXCOG00000008679.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157843"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160008">
        <property type="term" value="F:protein decrotonylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10000">
        <property type="entry name" value="HDAC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000006">
        <property type="entry name" value="Histone deacetylase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF14">
        <property type="entry name" value="HISTONE DEACETYLASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 20" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0678">Repressor</keyword>
    <keyword evidence="13 20" id="KW-0804">Transcription</keyword>
    <keyword evidence="12 20" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="24">
        <location>
            <begin position="33"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="21">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00061569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRNR" id="PIRNR037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="382" mass="42383" checksum="6C249AC9F6A6F243" modified="2018-12-05" version="1">MSAREHNDESSKRRVAYVYSPEYIETCDTLSKVPNRASMVHSLIEAYGLLPHMSVVKPRVATMEEMAMFHTDSYLEHLHKISRDGDNDDPQSADYGLGYDCPVVEGIFDYAAAVGGATLTAAECLLEQNCEVAINWAGGWHHAKKDEASGFCYVNDAVLGILKLREKYERVLYVDVDLHHGDGVEEAFSFTSKVMTVSLHKFSPGFFPGTGDVCDTGLGKGRWYAVNIPLEDGIQDDRYFQVFTSVMQEVQSQFNPEAVLMQLGADTMAGDPMCSFNMTPVGVAKCLLHVLQWQLPTLLLGGGGYNLANTARCWTFLTATVLGKSLSSEIPDHEFFTEYGPDYSLEISPSCRPDRNDSKHLERVISTIKGIKDFCHSKINTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LRP2</accession>
    <name>A0A3B5LRP2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Rho GTPase-activating protein 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Rho-type GTPase-activating protein 10</fullName>
        </alternativeName>
    </protein>
    <organism evidence="18 19">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">GTPase-activating protein that catalyzes the conversion of active GTP-bound Rho GTPases to their inactive GDP-bound form, thus suppressing various Rho GTPase-mediated cellular processes. Also converts Cdc42 to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death. Associates with MICAL1 on the endosomal membrane to promote Rab8-Rab10-dependent tubule extension. After dissociation with MICAL1, recruits WDR44 which connects the endoplasmic reticulum (ER) with the endosomal tubule, thereby participating in the export of a subset of neosynthesized proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LRP2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014733"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014919.1">
        <property type="protein sequence ID" value="ENSXCOP00000014733.1"/>
        <property type="gene ID" value="ENSXCOG00000011187.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159559"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01249">
        <property type="entry name" value="BAR-PH_GRAF_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000157">
        <property type="entry name" value="Putative rho GTPase-activating protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000006">
        <property type="entry name" value="Rho GTPase activating protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000001">
        <property type="entry name" value="Rho GTPase-activating protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000055">
        <property type="entry name" value="rho GTPase-activating protein 26 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047234">
        <property type="entry name" value="GRAF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047225">
        <property type="entry name" value="PH_GRAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12552">
        <property type="entry name" value="OLIGOPHRENIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12552:SF5">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="5" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="PH" evidence="16">
        <location>
            <begin position="233"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="17">
        <location>
            <begin position="357"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="15">
        <location>
            <begin position="636"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="543"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="592"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="597"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="624"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="694" mass="79195" checksum="B4A1E5D0256630A3" modified="2018-12-05" version="1">GINRFCNYVFLCSVCDYLSELGMAQRKFAQCLGEFQFEFIGDAKTDDEKCIDESLQEFSMFLKNLEDQRELMMRNITETLMKPLEKFRKEQLGNARAERKRYEKETEKYYSSLEKLLNLSAKKKEPQLQEADSQVEIMRQHFQEESLEYVCMLQEIQERKKFECVEPMLAFFQTVFTFYHQGYELAKDFDHYKTDLQINIQNTRSRFEGTRSEVHELMKRIKDTPQEYRQTSPISFEGYLYVQEKRPPPFGSSWVKRYCTFVKEQKILHMVTFDHRSGGKIGETESVTLKSCVRKTTDMLDRRFCLELDITDRPGTVLTVQALSEDDHKLWMHAMGGKEPIGCYLGRTLSKERGIDAQLDDVGLSFLKNSITAIETRGINDQGLYRVGGVSSKVQKLLSLMIDEKSNEMDLSASEDWDVKTITSALKLYLRSLPEPLMTYELYKEFINPAKGGSPESRIQAIHHLVHKLPERNRQVLGLLMKHLANVAAHSKQNLMTVANLGVVFGPTLMRPQEETVAAIMDLKFQNIVVEILIEHHEKIFTDSPELSPLSPTGPVLSAPTRQSKKVSRQNRPLAVYNPQVLDIQNDGSVLTADETSMGSNESVSSQSSSASTSELEKADHPTLTASLSSGSNSRVTNRKAKAVYPCEAEHETELSFQVGAIFSHVVPSREPGWLEGELEGKRGLIPENYVEML</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LS40</accession>
    <name>A0A3B5LS40_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LS40"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013625"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013792.1">
        <property type="protein sequence ID" value="ENSXCOP00000013625.1"/>
        <property type="gene ID" value="ENSXCOG00000010319.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
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        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:PRC1 complex"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:chromatin binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Chromobox protein homolog 7"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chromo-like_dom_sf"/>
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        <property type="entry name" value="Chromo_dom_subgr"/>
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        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
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        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
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        <property type="entry name" value="PcG_PRC1-like_component"/>
    </dbReference>
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        <property type="entry name" value="CHROMOBOX PROTEIN HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46389">
        <property type="entry name" value="POLYCOMB GROUP PROTEIN PC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CHROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Chromo" evidence="6">
        <location>
            <begin position="11"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="60" mass="7279" checksum="3FFF7CEA33BA760C" modified="2018-12-05" version="1">MELSAVGESVFAAESIIKRRIRRGRWEYLVKWKGWSQKYSTWEPEENILDERLFAAFEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LSS7</accession>
    <name>A0A3B5LSS7_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein 74</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
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            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027871984.1">
        <property type="nucleotide sequence ID" value="XM_028016183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LSS7"/>
    <dbReference type="Ensembl" id="ENSXCOT00000010984.1">
        <property type="protein sequence ID" value="ENSXCOP00000010859.1"/>
        <property type="gene ID" value="ENSXCOG00000008202.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114143844"/>
    <dbReference type="GeneTree" id="ENSGT00530000063880"/>
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        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029695">
        <property type="entry name" value="TMEM74-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16125">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16125:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14927">
        <property type="entry name" value="Neurensin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="265"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000010859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="30588" checksum="2CCCC06313C8CE64" modified="2018-12-05" version="1">MASPKLLPAKEAGKQSELNPEALDRVSGVQHARKSAGSTGEGLPGEGGCAPARGSGSRGRCLSAPRTAQRRAGAEGKLCPPPGGEEKLRVCCDEELETSFTFIDENVNLRLASPETGCKTSHRPARNGEPCSETLPELSFMSEDDLSFGEGSGSSVDYGFISAVTFLVTGISLVIISYAVPRDVEVDRDSVSAREMERLEMESARIGAHLDRCVIAGLCLLTLGGVVLSTLLMISMWKGEMYRRKLVAYSKRSAKLYGSISLKTRSSPSHSSARLSLEEEMVETLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LTX2</accession>
    <name>A0A3B5LTX2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA replication complex GINS protein PSF2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex is a core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the GINS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the GINS2/PSF2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027870907.1">
        <property type="nucleotide sequence ID" value="XM_028015106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LTX2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015453"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015645.1">
        <property type="protein sequence ID" value="ENSXCOP00000015453.1"/>
        <property type="gene ID" value="ENSXCOG00000011695.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114143246"/>
    <dbReference type="KEGG" id="xco:114143246"/>
    <dbReference type="CTD" id="51659"/>
    <dbReference type="GeneTree" id="ENSGT00390000007838"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11712">
        <property type="entry name" value="GINS_A_psf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21694">
        <property type="entry name" value="GINS_B_Psf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1020:FF:000001">
        <property type="entry name" value="DNA replication complex GINS protein PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.5.50:FF:000001">
        <property type="entry name" value="DNA replication complex GINS protein PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007257">
        <property type="entry name" value="GINS_Psf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056784">
        <property type="entry name" value="PSF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12772">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12772:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25005">
        <property type="entry name" value="PSF2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028998">
        <property type="entry name" value="GINS_Psf2_subgr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication complex GINS protein PSF2 N-terminal" evidence="8">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="GINS subunit" evidence="7">
        <location>
            <begin position="65"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR028998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="186" mass="21511" checksum="3776073E2293B193" modified="2018-12-05" version="1">MDPSEVEFLAEKEFVKIIPNFSLDKIYLIGGDLGPFNPGLSVDVPVWLALNLKQRQKCRILPPEWMDVEKLEEIRELERKEDTFTPAPSPYYMELTKLLLNHASDNIPKADEIRTLVKDIWDTRVAKLRLSADSFIRQQEAHAQLDNLTLMEINTIREFLLDSLNCMYKLRSNLQPGATKGQFADY</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LU16</accession>
    <name>A0A3B5LU16_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027878084.1">
        <property type="nucleotide sequence ID" value="XM_028022283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LU16"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000012811"/>
    <dbReference type="Ensembl" id="ENSXCOT00000012965.1">
        <property type="protein sequence ID" value="ENSXCOP00000012811.1"/>
        <property type="gene ID" value="ENSXCOG00000009681.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114147715"/>
    <dbReference type="KEGG" id="xco:114147715"/>
    <dbReference type="GeneTree" id="ENSGT00940000165065"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000017">
        <property type="entry name" value="Homeobox protein antennapedia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050296">
        <property type="entry name" value="Antp_homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017995">
        <property type="entry name" value="Homeobox_antennapedia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45659">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45659:SF9">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-B6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00025">
        <property type="entry name" value="ANTENNAPEDIA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="185"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="187"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="247"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000012811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="273" mass="29854" checksum="1297C11CA19EF7CE" modified="2018-12-05" version="1">MSSYFVNSSFPVIPGTGGGGQTASAEPFLGQISLYSSGYADHPLRHYPGAAAVTAAAAVAYSAHQEKSYPASTYYQQAANGAYSGHRVTGVAVGGSTGPGACDYATAAAAAAAAATNFYRDKDHPCSLDEHQLALSQDGMHRKMECAGLGAKGLFGESMDDKQTAPTPIYPWMQRMNACNGTFGSPGRRGRQTYTRYQTLELEKEFHFNRYLTRRRRIEIAHALCLTERQIKIWFQNRRMKWKKENKLINSSSSSSSSSFTLNGTDEEEKRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUB5</accession>
    <name>A0A3B5LUB5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">procollagen galactosyltransferase</fullName>
            <ecNumber evidence="7">2.4.1.50</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(5R)-5-hydroxy-L-lysyl-[collagen] + UDP-alpha-D-galactose = (5R)-5-O-(beta-D-galactosyl)-5-hydroxy-L-lysyl-[collagen] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12637"/>
            <dbReference type="Rhea" id="RHEA-COMP:12752"/>
            <dbReference type="Rhea" id="RHEA-COMP:12753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="ChEBI" id="CHEBI:133442"/>
            <dbReference type="ChEBI" id="CHEBI:133443"/>
            <dbReference type="EC" id="2.4.1.50"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 25 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.50" evidence="7"/>
    <dbReference type="RefSeq" id="XP_027898975.1">
        <property type="nucleotide sequence ID" value="XM_028043174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUB5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014440"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014625.1">
        <property type="protein sequence ID" value="ENSXCOP00000014440.1"/>
        <property type="gene ID" value="ENSXCOG00000010951.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114160532"/>
    <dbReference type="KEGG" id="xco:114160532"/>
    <dbReference type="CTD" id="565862"/>
    <dbReference type="GeneTree" id="ENSGT01030000234558"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050211">
        <property type="term" value="F:procollagen galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06532">
        <property type="entry name" value="Glyco_transf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000048">
        <property type="entry name" value="Glycosyltransferase 25 family member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050757">
        <property type="entry name" value="Collagen_mod_GT25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002654">
        <property type="entry name" value="Glyco_trans_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10730:SF28">
        <property type="entry name" value="PROCOLLAGEN GALACTOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10730">
        <property type="entry name" value="PROCOLLAGEN-LYSINE,2-OXOGLUTARATE 5-DIOXYGENASE/GLYCOSYLTRANSFERASE 25 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01755">
        <property type="entry name" value="Glyco_transf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017295427" description="procollagen galactosyltransferase" evidence="10">
        <location>
            <begin position="22"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 25" evidence="11">
        <location>
            <begin position="329"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="573"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="573"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="583"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="611" mass="71560" checksum="4BD27F38D7A5875D" modified="2018-12-05" version="1">MHGLAVLPAALLLLLLRCCSSVRGYFAEERWSPESPLLAPRVLLALICRNSEHSLPYFLGTIERLKYPKDRMALWVATDHNEDNTTVILRDWLLKVENHYHYVEWRPKEEPSRYEDEDGPKHWTDLRYEHVMKLRQGALESAREMWADYLMLVDCDNLLTDRDVLWKLIKENKTIIAPMLESRGAYSNFWCGMTSQGYYRRTPAYLPIRKQVRKGCFAVPMVHSTFLIDLRKQATRQLAFHPPHPEYSWAFDDIIVFAFSAQMADVQMFVCNRESYGYIPVPLRSHNTLQDEADSFLHSLLEVNVRNPPLMPSKYIRVPRKKPDKMGFDEVFMINLPRRTDRRERMLKALHEQKIACKIIAAVDGKAMNVSEMHALGIHMLPGYQDPYHGRPLTKGELGCFFSHYNIWKEIVDRRLETSLVIEDDLRFEVFFKRRLMNLMSEIEHEGLDWDLIYIGRKRMQVDHKEKVVPNIHNLVEADYSYWTLGYMISLQGAEKLLKAEPLKRILPVDEFLPIMYNKHPLSDYMEQFESRDLKAFSAEPLLVFPTHYTGDPGYISDTETSTVWDNDKVRTDWDREARSGKTQEQAEISREAQNTDVLHSPLDSTARDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUJ5</accession>
    <name>A0A3B5LUJ5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphorylase b kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Glycan biosynthesis; glycogen metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Cell membrane</location>
            <topology evidence="2 7">Lipid-anchor</topology>
            <orientation evidence="2 7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the phosphorylase b kinase regulatory chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUJ5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000011494"/>
    <dbReference type="Ensembl" id="ENSXCOT00000011626.1">
        <property type="protein sequence ID" value="ENSXCOP00000011494.1"/>
        <property type="gene ID" value="ENSXCOG00000008676.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183118"/>
    <dbReference type="UniPathway" id="UPA00163"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005964">
        <property type="term" value="C:phosphorylase kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008734">
        <property type="entry name" value="PHK_A/B_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10749:SF4">
        <property type="entry name" value="PHOSPHORYLASE B KINASE REGULATORY SUBUNIT ALPHA, SKELETAL MUSCLE ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0321">Glycogen metabolism</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH15-like" evidence="8">
        <location>
            <begin position="20"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000011494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="228" mass="25857" checksum="C48179C1BF410C7B" modified="2018-12-05" version="1">SPWKKRDLPLVSMRSRSNSGVKLDNYARIVQETILQHQDPVTGLLPGSTDHPDAWVRDNVYSIASVWALSLAYRKNADRDEDKAKAYELEQSVVKLMRGVLQCIMRQLDKVEKFKYSRSTKDSLHAKYNTRTCATVVGDDQWGHLQVDATSLFLLFLAQMTASGLHIIYTQDEVDVIQNLMFYIEAAYKVADYGMWERGDKTNQGITEINASSIGTAKVSSRFPESQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LUY4</accession>
    <name>A0A3B5LUY4_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Inactive rhomboid protein</fullName>
            <shortName evidence="11">iRhom</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Rhomboid family member</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Rhomboid veinlet-like protein</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 11">Regulates ADAM17 protease, a sheddase of the epidermal growth factor (EGF) receptor ligands and TNF, thereby plays a role in sleep, cell survival, proliferation, migration and inflammation. Does not exhibit any protease activity on its own.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Endoplasmic reticulum membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LUY4"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014680"/>
    <dbReference type="Ensembl" id="ENSXCOT00000014866.1">
        <property type="protein sequence ID" value="ENSXCOP00000014680.1"/>
        <property type="gene ID" value="ENSXCOG00000011142.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156278"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050709">
        <property type="term" value="P:negative regulation of protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042058">
        <property type="term" value="P:regulation of epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000001">
        <property type="entry name" value="Putative inactive rhomboid protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051512">
        <property type="entry name" value="Inactive_Rhomboid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022241">
        <property type="entry name" value="iRhom1_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45965">
        <property type="entry name" value="INACTIVE RHOMBOID PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45965:SF4">
        <property type="entry name" value="INACTIVE RHOMBOID PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12595">
        <property type="entry name" value="iRhom1-2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="321"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="573"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="606"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="634"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="664"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="687"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="721"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="Inactive rhomboid protein 1/2 N-terminal" evidence="14">
        <location>
            <begin position="85"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="13">
        <location>
            <begin position="568"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="772" mass="87961" checksum="0E2C9BDC6792C982" modified="2018-12-05" version="1">VWSGIASMAEPRRESTSSLQRKKPPWLRLDIPTGVACSPDSLPHVRASYEDHQQFALNVSDVSLKISPEKLLLSGVRGTADWFGVSKDGDATQKWQRKSLRHCSLRYGKLKPQVIREMDLPSQDNISLTSTETPPPLYIVDPLARGRAFRMVEEVDGYSVPPTPITPGATSLCSFTSSRSGLNRLPRRRKRESVAKMSFRAAAALVKGRSLRESTLKRAQRRSFTPASFMEEDVVDFPDELDTSFFARVRPPKVPLRQEVVSVHGQRRGQRIVVPVKKLFAREKRPYGLGMVGKLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWITFVHLLITILAVCIYGIAPVGFSQHETVDSVLRNKGVYENVKFVQQENFWIGPGSESLIHLGAKYSPCMRQDEQVHNLIREKKAVERNSACCVRNDRSGCVQTSEEECSSTLAEWVKWPNPIFSTPQFNGKDRQYGSVCHQDPGICLEPASVSPHEWPDDITKWPICTRYNTGNHTNLPHIDCTITGRPCCIGTKGRCEITSREYCDFMKGYFHEEATLCSQVHCMDDVCGLLPFLNPEIPDQFYRLWLSLFLHAGILHCMVSVAFQMTILRDLEKLAGWLRISIIYILSGITGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILAQPWRAFIKLLCVVLFLFAFGLLPWIDNFAHICGFISGFFLSFAFLPYISFGRMDLYRKRCQIIVFLLVFVGLFSGLVVLFYVYPIKCDWCELITCIPFTDKFCEKYDLNAHLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LVT5</accession>
    <name>A0A3B5LVT5_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TSC complex subunit 1a</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LVT5"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000015262"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015453.1">
        <property type="protein sequence ID" value="ENSXCOP00000015262.1"/>
        <property type="gene ID" value="ENSXCOG00000011559.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014148"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033596">
        <property type="term" value="C:TSC1-TSC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007483">
        <property type="entry name" value="Hamartin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15154">
        <property type="entry name" value="HAMARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15154:SF2">
        <property type="entry name" value="HAMARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04388">
        <property type="entry name" value="Hamartin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000015262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="40426" checksum="0538C3EC12976436" modified="2018-12-05" version="1">MSREPLSVSDLLPAGHSSCWLGLTVVLFADRGGAVLSSLVEHYLDSSSSQVLLLLSSIREPHHKVLLEKLNEALTRPGNRAAAIALLGHLIRKQPPWIHHISQAPVLPTLLRCLKTDGDVAVLVSGVLVLVALLPMIPQAGKQHIYDFFDVFGRLASRSYRNPGQVSAAYLVHLHAAVYSLFHRLYGMFPCNFVSYLRLHYGMKENLDTFQEVVKPMLEHVRIHPELVTGTQDSELDPWRCCEVHDIIMECCRLSLDPLEDACCPSPQPHLDLSPAPQVWQPISSSGEEQNHRGPSRPNWARTGQLAVGSGTSRKTRFCWEPEHPACYSTCVFRLMMSHGAPPLNVAWPRPLLSLHLQASPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LW59</accession>
    <name>A0A3B5LW59_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Phosphomannomutase</fullName>
            <ecNumber evidence="5 13">5.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>alpha-D-mannose 1-phosphate = D-mannose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11140"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="EC" id="5.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the eukaryotic PMM family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.8" evidence="5 13"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5LW59"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013551"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013718.1">
        <property type="protein sequence ID" value="ENSXCOP00000013551.1"/>
        <property type="gene ID" value="ENSXCOG00000010266.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002918"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00424"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02585">
        <property type="entry name" value="HAD_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005002">
        <property type="entry name" value="PMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043169">
        <property type="entry name" value="PMM_cap"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466:SF2">
        <property type="entry name" value="PHOSPHOMANNOMUTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03332">
        <property type="entry name" value="PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="250" mass="28734" checksum="AC066139C5F7DB59" modified="2025-10-08" version="2">MGRNTADTSTLCLFDVDGTLTAARQRVTPDMKAFLEKLRTRVRVGVVGGSDLSKIKEQLGDDVIQKSDYVFAENGLVAYRHGQLHSIQSIQAHMGEELLQDFINFCLNYMAKIQLPRKRGTFIEFRNGMLNVSPIGRSCSQEERQEFYELDQREKIREKFVSVLKEEFRGKGLSFSIGGQISFDVFPDGWDKRYCLDIVDKDNYSTVHFFGDKTKPVRTLSFVCSTTALSLSRMFCCWVLWNQNQNLCRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LWL8</accession>
    <name>A0A3B5LWL8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_027899016.1">
        <property type="nucleotide sequence ID" value="XM_028043215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LWL8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000013721"/>
    <dbReference type="Ensembl" id="ENSXCOT00000013889.1">
        <property type="protein sequence ID" value="ENSXCOP00000013721.1"/>
        <property type="gene ID" value="ENSXCOG00000010382.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114160560"/>
    <dbReference type="GeneTree" id="ENSGT00940000159473"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008188">
        <property type="term" value="F:neuropeptide receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15919">
        <property type="entry name" value="7tmA_GPR139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037486">
        <property type="entry name" value="GPR139"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052477">
        <property type="entry name" value="Orphan_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46272:SF3">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 139-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46272">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="39"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000013721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="369" mass="41421" checksum="92392BBECBC7E0EE" modified="2018-12-05" version="1">MEHSHIFPVSHHNRSTGGCPLGPLPVIYYSVLLCLGLPANILTVVILSQLVLRRQKSSYNYLLALAAADILVLLLIVFVDFILEDFILASFLPPSINNAVQVLEFSSIHTSIWITVPLTIDRYIAVCHPLRYHTVSYPARTRKVIFAVYVGCLLSAAPYYWWPEFWHSLPGVGKSNEGGGGGGESSRRTVAEHVLVWAHCTTVYLLPCTVFFSLNTGIVRKLRKRRNCFRLRGYSTGKTTAILLAITSVFAILWAPRTLLILYHFYSPPPTSQSAGRLLHVLTDVANMLALLNTGVNFFLYCFISKRFRAMATNMLRALIHCRKQPKPFYASHNFSITSSPWISPANSHCIKMLVYQYDKNGKPICISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZT0</accession>
    <name>A0A3B5LZT0_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">WD repeat-containing protein 35</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">As a component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs), it is involved in ciliogenesis and ciliary protein trafficking. May promote CASP3 activation and TNF-stimulated apoptosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the IFT complex A (IFT-A) complex. IFT-A complex is divided into a core subcomplex composed of IFT122:IFT140:WDR19 which is associated with TULP3 and a peripheral subcomplex composed of IFT43:WDR35:TTC21B. Interacts directy with IFT122, ITF43 and TTC21B. Interacts with IFT43. Interacts with CFAP61.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
            <location evidence="12">Cytoskeleton</location>
            <location evidence="12">Microtubule organizing center</location>
            <location evidence="12">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
            <location evidence="12">Cytoskeleton</location>
            <location evidence="12">Cilium basal body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZT0"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014861"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015047.1">
        <property type="protein sequence ID" value="ENSXCOP00000014861.1"/>
        <property type="gene ID" value="ENSXCOG00000011261.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155745"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030991">
        <property type="term" value="C:intraciliary transport particle A"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097730">
        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035721">
        <property type="term" value="P:intraciliary retrograde transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061512">
        <property type="term" value="P:protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.470:FF:000004">
        <property type="entry name" value="WD repeat-containing protein 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000187">
        <property type="entry name" value="WD repeat-containing protein 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056158">
        <property type="entry name" value="Beta-prop_IFT121_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056159">
        <property type="entry name" value="Beta-prop_IFT121_TULP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039857">
        <property type="entry name" value="Ift122/121"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057979">
        <property type="entry name" value="TPR_IFT121"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056157">
        <property type="entry name" value="TPR_IFT80_172_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057361">
        <property type="entry name" value="TPR_WDR35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017233">
        <property type="entry name" value="WDR35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056170">
        <property type="entry name" value="Znf_IFT121-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12764:SF5">
        <property type="entry name" value="LD29485P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12764">
        <property type="entry name" value="WD REPEAT DOMAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23390">
        <property type="entry name" value="Beta-prop_WDR35_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24797">
        <property type="entry name" value="Beta-prop_WDR35_TULP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25768">
        <property type="entry name" value="TPR_IFT121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23387">
        <property type="entry name" value="TPR_IFT80_172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25170">
        <property type="entry name" value="TPR_WDR35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23145">
        <property type="entry name" value="Zf_2nd_IFT121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037536">
        <property type="entry name" value="WD_repeat_p35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6 12" id="KW-0969">Cilium</keyword>
    <keyword evidence="5 12" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="2 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="IFT121/TULP4 N-terminal" evidence="17">
        <location>
            <begin position="1"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="67"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="IFT121 second beta-propeller" evidence="16">
        <location>
            <begin position="451"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="IFT80/172/WDR35 TPR" evidence="15">
        <location>
            <begin position="651"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="IFT121-like TPR repeats" evidence="18">
        <location>
            <begin position="974"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="domain" description="IFT121-like zinc finger" evidence="14">
        <location>
            <begin position="1091"/>
            <end position="1133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00070596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR037536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF23390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF24797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF25768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1134" mass="127938" checksum="14CFAAF0B79FE55B" modified="2018-12-05" version="1">MFIYLSKKIAIPNNVHLKCLSWNKHQGFIACGGEDGLLRVLKLETQTDDSRLKGLAAPSNLSMNQTLEGHSGAVQVVTWNERNEKLTTSDQNGLIIVWMLYKSAWYEEMINNRNKSVVRSMSWNADGQKICIVYEDGAVIVGSELKGSQLAHVAWSPDSKILLFGMANGEVHIYDNQGNFIMKMNISCLAGATGAVSLAGIHWYAGAGGYSEPDCPCLAICFDNGRCQIMRYENDENPVYIDTLMNVISIQWNHCGSVLAVAGSLRATNMDKEYNVVQFYTPFGEHLRTLKVSGKHLSGISWEGGGLRIALAVDSYIYFANIRPDYKWGYCCSTVVYAYTKPERQEYCVVYWDTKNNEKFVKYVKNLTSITTSGDFCILASKADEKQPQYVLILCNSIGTPLDSKYIDIDPLFVTMTRTHVITASKEAFYLWQYRVAKKLTALEINQVTRTKKGDPICCITATDKTLIVGRESGIIQRYTLPNVALVQKYSSNSRAHYLSLNCNSSRLAIIDISGLLTLLDLEVRPAGSEGTGDQAAAGDPSKFERKDVWDLKWANDNPDLFAVMEKTRMYVFRNLDPEEPIQTSGYICNFEDLEIKSVLLDEIMKNPERPNKDNLFNFEIRSLRDSRALIEKVGIEDASQFIEDNPHPRLWHLLAEAALQKLDLKTAEEAFVRCKDYPGIQFVKRLGNLQSEPMKQAEVAAYFGRFEEAESMYLNMDRRDLAINLRIKLGDWFKVLQLLDTGSGYSDDALLAQANNGIGDYFADRQKWVKAVPYYLKGRNQERLADCYYKLEDYNGLELLAADLPENHKLLPEIGEMFATVGMCEQAVKAYLKSNQPKAAVDTCVHLNQWNKAVELARTHNMKEIKSLLSKYASHLLEENKTLEAVELYRKAHHFLDAAKLMFKIADKEAKKMSRPLRVKKLYVLAAQLVEDYHEQMKASQQIKAKGKKSEATFALAGLLEEDATSSDNRIIDSAWRGAEAYHFFLLAQRQLHAGYPENATRTALHLREYEDIIPAVEIYSLLALCSAANRSFGTCSKAFIKLESLESLSSEQQQLYEDLALQIFTKYPPKDSRVVEAQGSSEGAEGKLPTCIVTGRPIQEYQFWMCSVCKHCAVEQEISKYNCCPLCHSAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5LZV9</accession>
    <name>A0A3B5LZV9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CCHC-type zinc finger, nucleic acid binding protein a</fullName>
        </submittedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_027866912.1">
        <property type="nucleotide sequence ID" value="XM_028011111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5LZV9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000014891"/>
    <dbReference type="Ensembl" id="ENSXCOT00000015077.1">
        <property type="protein sequence ID" value="ENSXCOP00000014891.1"/>
        <property type="gene ID" value="ENSXCOG00000011191.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114140827"/>
    <dbReference type="KEGG" id="xco:114140827"/>
    <dbReference type="CTD" id="326846"/>
    <dbReference type="GeneTree" id="ENSGT00950000183041"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048703">
        <property type="term" value="P:embryonic viscerocranium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060322">
        <property type="term" value="P:head development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014036">
        <property type="term" value="P:neural crest cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050727">
        <property type="term" value="P:regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000002">
        <property type="entry name" value="cellular nucleic acid-binding protein-like isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000026">
        <property type="entry name" value="cellular nucleic acid-binding protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025836">
        <property type="entry name" value="Zn_knuckle_CX2CX4HX4C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47103">
        <property type="entry name" value="DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47103:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14392">
        <property type="entry name" value="zf-CCHC_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="43"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="107"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="125"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="6">
        <location>
            <begin position="147"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000014891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="166" mass="18339" checksum="5FEE7DFD0EADA5F8" modified="2018-12-05" version="1">MEMSSNSECFGCGRSGHWVKHCPNASGARGRGRGRGRGKELFCYRCGDQGHMARDCDQTEDACYNCHRSGHISRDCKEPRKEREQLCYTCGKAGHMARDCDHANEQKCYSCGGFGHIQKLCDKVKCYRCGEIGHVAVHCSKASETNCYNCGKAGHLAKECTIEATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M2Q6</accession>
    <name>A0A3B5M2Q6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Leucine-rich pentatricopeptide repeat containing</fullName>
        </submittedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="STRING" id="32473.ENSXCOP00000017702"/>
    <dbReference type="Ensembl" id="ENSXCOT00000017926.1">
        <property type="protein sequence ID" value="ENSXCOP00000017702.1"/>
        <property type="gene ID" value="ENSXCOG00000013343.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00960000186682"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070129">
        <property type="term" value="P:regulation of mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033490">
        <property type="entry name" value="LRP130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033443">
        <property type="entry name" value="PROP1-like_PPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46669">
        <property type="entry name" value="LEUCINE-RICH PPR MOTIF-CONTAINING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46669:SF1">
        <property type="entry name" value="LEUCINE-RICH PPR MOTIF-CONTAINING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01535">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17177">
        <property type="entry name" value="PPR_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="232"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="PROP1-like PPR" evidence="5">
        <location>
            <begin position="259"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="267"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="2">
        <location>
            <begin position="739"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="646"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000017702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1384" mass="154830" checksum="359FC6A0C5610C0A" modified="2018-12-05" version="1">VISSTAACPATGARTTPPAPRSFTRSLSCRKNMAALLRSARLLKFSPSGLLQIPGSRRSGAPLSRLYSGVLVGRSTGVCSRQLGRVSDSLEIYSVCVRSYAVATEQKDEIGLAVRSKQAQQFDWALTKLDSSVRRTGRITKTLLMRIFHDICRTGYPSSNHALLLLRSCGSLLPEVPMEERNDLKLNLGIIFFDIYDVSHYNALLKVYLQNEFKFSPTDFLAKMEAANVQPNRVTYQRLIAAYCQNGDIEGASTILGFMKSKDLPITEAVFSSLITGHSRAGDIESAANILSVMRGAGVEPSPDTYVTLLNAYGEKGDLESMKKTLEAAESADCSLMDRDIMQVILTLAKAGQQQHIPEMIERLRHERGYIPDAMNLCLSLITQGHEDTAFSLLKTFPTLQSETNDSSILGNFFLRHCVNMDTALEKVVRYCKELQELNLHTSSFTFTLSCALEAQKLGMSLELMKTVKEQNLPVRPHYFWPLLTQPLKENNIFSIIQILLGVSPDLETLATYILPVFPSLEAALKTLQDSGVSVDSEAFVCAEIRTLAASNLAKVYTLSKSTHIDSNGVFNKMLKLFTFHAHLAVFVILTFEFLSIFFLQNVTISLNIYRGIKNLLEAYHCPELIKVQIRTHITSVSKVDFVKLFHHDENRVLALERKLAELKAENKPLSSILKQTIHALSAEENLQRALELKQQHEDDMSVGAYATLIGLCCRHNNTEEALNLKRELSRKDSLVTLDPIKYMALIKTLSLNDKVEEAVDILKEMKEKDVMLNDSHITSVFHMLNTVAAKGGIPTVRRLQDTIFTLGLAKPTANLCSPVVTFYLESKDASGALDALLECHKLYNQLPRIHDVIVALVEKGDTDLLQKVTDFVSQERGEMMMLYDLFFAFLQTGRYREARKIIETPGLRAKPGRLQWFGEKCIASNQMGALEQMVEMTSKLFECDRDEMYSYILRLCKETNDWQKAEATWTKMQEENVIPRESTLRLLADILKSNRQEVPFEVPEVKLQSQPPPLEAERSDRYQGRLLTLCKKGKAKEALGVVKEVDEKGVVLGGLSYDHVIRALLAQGHIEDAMVVKQLAQSHLPGFKLSEIANNLLIISYKALEKLKSMLQLDHVPSHLAITRLVQALGNQGDVAGIQEVESVAKTLGTNVNLSSMVFVNNTALAHIAKGDVESAVELLEGIYMSPDSSNPSMSFVFRKVLESGNDKALDKLSAMAERLANHFACYRPVSDLFLLLLDMDKVEDAKFLLARFNVLAEQKEMLLSFVAQSAQSPGQVRKIKNLLSLVPDVAEKEIVYSYLMKCHALDKDLPSGKALYEQMQREGVVTDNLFLKRLAVLYREAGETAPFPEPPVSERRADPNQNSKTWDISVENTEDFYTFLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3L8</accession>
    <name>A0A3B5M3L8_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SPT3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3L8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018753"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018989.1">
        <property type="protein sequence ID" value="ENSXCOP00000018753.1"/>
        <property type="gene ID" value="ENSXCOG00000014116.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010738"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07978">
        <property type="entry name" value="HFD_TAF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000023">
        <property type="entry name" value="transcription initiation protein SPT3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003195">
        <property type="entry name" value="TFIID_TAF13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID/SUPT3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380:SF16">
        <property type="entry name" value="TRANSCRIPTION INITIATION PROTEIN SPT3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02269">
        <property type="entry name" value="TFIID-18kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="313" mass="35621" checksum="F83456250FDF203B" modified="2018-12-05" version="1">QVGKSDVMAGSVTSSSKERPASRTSFIPELQSMMFALGDTRRPLYETAALVEDIVHTQLINMLHQACEGASLRGSRVISAEDILFLMRRDKKKLARVLKYLQFRDYKSKLLKNLEEEDVQQEPGGLAAGGNQRRQRLAQDFLAWMDQTGEFLSLAEKQEVDPVKQERMERLERHTRNMDPAQYAEFCESRQLSFAKKASKFRDWLDCSSLELKPNSIATEILSYLAYETVAQIVDLSLLVKQEMTVKNNPVSHVISASYIHYNTHTESSPATVEPPSGAIQPCHIREAIRRYNYRHTVGTHAYRRSGMAFLTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M3U9</accession>
    <name>A0A3B5M3U9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ATP-binding cassette, sub-family B (MDR/TAP), member 10</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M3U9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016101"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016304.1">
        <property type="protein sequence ID" value="ENSXCOP00000016101.1"/>
        <property type="gene ID" value="ENSXCOG00000012168.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157680"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030218">
        <property type="term" value="P:erythrocyte differentiation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070455">
        <property type="term" value="P:positive regulation of heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd18573">
        <property type="entry name" value="ABC_6TM_ABCB10_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000048">
        <property type="entry name" value="ATP-binding cassette sub-family B member 10, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF1">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002773">
        <property type="entry name" value="ABC_prm/ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="131"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="453"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="693" mass="75422" checksum="5B6A5D20FDECDD83" modified="2018-12-05" version="1">MRLLIRMNRMHFNKLKLAQSRLLCLEHRAILSASHRRRRQSAGLKTSACPPPSPVLSVLSCSAFTSGLSSLRGRTVAHTPVAFASSSAASTPAESAKSQTDAESQRIPTTVPLDDVKRILDLAHPERWRLAAGVGFLTVSSAVTMSAPFFLGRVIDTIYASGADTETMTASLTSLCLMLTGVFLCGGAANAARVYLMQASGQQIVRNLRATVFSAVLRQEVGFFDKNRTGELINRLSSDTSVVGHSITDNLSDGLRAVAQAVAGVSMMFYVSPSLASFVLMIVPPIAVLAIIYGRYLRSISKRTQDALAQATQLAEERIGNIRTVRAFGKELSEVDKYTQKTDYVLSLARKEAVIRAGFFGVTGLSGNLMILSVLYKGGLLTASQHMTVGELSSFLMYTFWVGISIAGLSSFYSELMKGFGAGARLWELLDRKPEFPLNEGRILSPDHLKGQLEFSNVSFSYPTRKDAPIFQNLNLLVPAGTIMAVVGPSGSGKSSLVSLLLRLYDPDAGTITIDGHDIRDLNPYWLRNHIGTVSQEPVLFSCSIRENIAYGAIDPDAVTIQDIHSAARVANAYDFIQSFPKGFDTVVGEKGVLLSGEELGQQQDGLRATALDAENEFLVQEALESLMEGRTVMIIAHRLSTIKNADSVAVLDQQRVAECGQPTELLENRQGLFRKLMEKQAFLQEEQQRALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4J1</accession>
    <name>A0A3B5M4J1_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pectinacetylesterase family. Notum subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4J1"/>
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    <dbReference type="Ensembl" id="ENSXCOT00000016547.1">
        <property type="protein sequence ID" value="ENSXCOP00000016341.1"/>
        <property type="gene ID" value="ENSXCOG00000012343.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990699">
        <property type="term" value="F:palmitoleyl hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004963">
        <property type="entry name" value="PAE/NOTUM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562:SF10">
        <property type="entry name" value="CARBOXYLESTERASE NOTUM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21562">
        <property type="entry name" value="NOTUM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03283">
        <property type="entry name" value="PAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="119" mass="13290" checksum="6953FA6CE5AC26DC" modified="2018-12-05" version="1">GMVQDSTAGSRTTGQQTDEMRLQFLRSSQVTCNDGSAAGFYLKEFRGSRRWLLFLEGGWCCYSKETCDSRYQNIPRLMSSSGWPQTKRGTGILSSQAEENPHWHNANLVSNSGNLVCSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M4L9</accession>
    <name>A0A3B5M4L9_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="4">WW domain binding protein 1-like a</fullName>
        </submittedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M4L9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000018392"/>
    <dbReference type="Ensembl" id="ENSXCOT00000018624.1">
        <property type="protein sequence ID" value="ENSXCOP00000018392.1"/>
        <property type="gene ID" value="ENSXCOG00000013850.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183109"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021684">
        <property type="entry name" value="WBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051994">
        <property type="entry name" value="WW_domain-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16209">
        <property type="entry name" value="VESICULAR, OVEREXPRESSED IN CANCER, PROSURVIVAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16209:SF4">
        <property type="entry name" value="WW DOMAIN BINDING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11669">
        <property type="entry name" value="WBP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5017366293" evidence="3">
        <location>
            <begin position="23"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000018392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="254" mass="28720" checksum="927E86B0389DFF7E" modified="2018-12-05" version="1">MQGWFSTTFIFLAGLVCKGVNNQSYICESGHCCGQAECCSNIYELWWFWLVWAVIIILTGFCVWQHWRAKQRFQQQRRQNEINLIAYREVHNNSQLPLYLRLLPVYLLPAYEETVTRPATPPPPYTPLQLVPPSSDPPEEVYRPHSAVSIPCDANVALSGTPETTPFYSSSTNGPNKDSTAGRYRRFTGDSGIEVCEGQEHPWDQHEFFDREETLEEEGLRGKDEPDDCTDPQTAEWGLAGGPAADDTQTKSPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M558</accession>
    <name>A0A3B5M558_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ENTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M558"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019298"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019535.1">
        <property type="protein sequence ID" value="ENSXCOP00000019298.1"/>
        <property type="gene ID" value="ENSXCOG00000014515.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158217"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF50">
        <property type="entry name" value="EPSIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="1">
        <location>
            <begin position="12"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="84" mass="9619" checksum="F6E498DB1BD74664" modified="2018-12-05" version="1">MTTSALRRQVKNIVHNYSEAEIKVREATSNDPWGPSSSLMSEIADLTFNVVAFAEVMGMVWKRLNDSGKNWRHVYKVCFSTPRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M5S2</accession>
    <name>A0A3B5M5S2_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">lysophospholipase</fullName>
            <ecNumber evidence="3">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="22 23">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40807"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28610"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:40808"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>1-hexadecanoyl-sn-glycero-3-phosphate + H2O = sn-glycerol 3-phosphate + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49092"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57518"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:49093"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40435"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:40436"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:15178"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type III membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NTE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M5S2"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019518"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019758.1">
        <property type="protein sequence ID" value="ENSXCOP00000019518.1"/>
        <property type="gene ID" value="ENSXCOG00000014664.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159130"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd07225">
        <property type="entry name" value="Pat_PNPLA6_PNPLA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000010">
        <property type="entry name" value="neuropathy target esterase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000012">
        <property type="entry name" value="neuropathy target esterase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000001">
        <property type="entry name" value="neuropathy target esterase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000022">
        <property type="entry name" value="Patatin like phospholipase domain containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056556">
        <property type="entry name" value="NTE1_P-loop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF26">
        <property type="entry name" value="PATATIN-LIKE PHOSPHOLIPASE DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24179">
        <property type="entry name" value="NTE_Ploop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 17" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="11 17" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="20">
        <location>
            <begin position="124"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="20">
        <location>
            <begin position="407"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="20">
        <location>
            <begin position="528"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="21">
        <location>
            <begin position="825"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1161"/>
            <end position="1203"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="17">
        <location>
            <begin position="829"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="17">
        <location>
            <begin position="856"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="17">
        <location>
            <begin position="983"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="318"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="1161"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="17">
        <location>
            <position position="858"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="983"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="1203" mass="134701" checksum="B2918828DEF671FD" modified="2018-12-05" version="1">QDRFENLKTFVEEELQTSMMVGLVIGAGVAIVLIAILIFFILRRIRLRQSTSSLVGASSSSSRPRLKKKQKMLNIAKILRFKKEVPILQAKEPPPSVLEADLTEFDVANSHLPSEVLYMLKNVVLGHFEKPLFLELCKHMVFLQFQQGEHVFRPGQPDSSIYVVQDGKLELCLTGDGKESVVKEVFAGDSVHSLLSILDVITGHQKPYRTMSARAAEVTTVLRLPVEAFLAIFEKYPESLVRVVQQIIMVRLQRVTVLALHNYLGLTNELFSHLLFADQGKEGGPPNLSRTISMPVDISIQKSVKSDFDMAFERGRISTSAEDGNTPPTFSRSVSHEQRERKVTVEEVPSGIYLYPEEESGVDNIFTPSSSRCNASLFEDAQKEVLRLMQIEDPGLLRGKASMHHAKAGAVLARQGDQDVSLHFVLSGCLHVYQRMIDKQEAVCLFVTHPGEMVGQLAVLTGEPLIFTIKAVRDCTYLKISKSDFYIMKEQPSVVLSVAHTVAARMSAFVRQMDFAIDWMAVEAGRALYQDDQSDCTYIILNGRLRSVIRKANGKKELVGEYGRGDLIGVEALTKQPRATTVHAVRDTELVKLPEGTLNNIKRRYPQSALSLPSMRKSADVTNPASNLSTVAVLPVGDEVPISAFNLELSHALSAIGPTLLLTSDIIRERLGASALDIYEYRLSGWLAQQEDINRIVLYQTDSSMTPWTQRCIRQADCILIVGVGDQEPALGELEQMLENTAVRALKKLILLHREDGPGPSRTVEWLNMRSWCSGHLHLKCPRRVFSRRSPTKLREVYEKVFQKTADRHSDFSRLARVLTGNSIALVLGGGGAGCSHVGVIKAMEESGIPIDIVGGTSIGSFIGALYAEERSAVRTKQRAKEWSKAMNSVFKTVLDLTYPITSMFSGSAFNTSIYNVFEDKQIEPVFDLQDLWLPYFNVTTDITASAMRVHLDSLWRYVRASMTLSGYLPPLCDPKDGNLLMDGGYINNLPDIARNVGTRTVIAIDVGSQDETDLCNYGDSLSGWWLLWKRINPWAEKVKVPDMAEIQSRLAYVSCVRQLEVVKKSAYCEYIRPPIDRFKTMDFGKFDEIYQEIGYQHAKMLFTGWARGDIIMNMLRDHRSADYNNSKRTDCPGADFTDLAEIVSRIEPVQSYVATEDESDYLTEYEEDGMDTVREEEGDEDEHEEAEDHSPGEWGQNGVFQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M6V9</accession>
    <name>A0A3B5M6V9_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Beta-crystallin A2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Beta-A2 crystallin</fullName>
        </alternativeName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Crystallins are the dominant structural components of the vertebrate eye lens.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homo/heterodimer, or complexes of higher-order. The structure of beta-crystallin oligomers seems to be stabilized through interactions between the N-terminal arms.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta/gamma-crystallin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_027877976.1">
        <property type="nucleotide sequence ID" value="XM_028022175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A3B5M6V9"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000019237"/>
    <dbReference type="Ensembl" id="ENSXCOT00000019474.1">
        <property type="protein sequence ID" value="ENSXCOP00000019237.1"/>
        <property type="gene ID" value="ENSXCOG00000014463.1"/>
    </dbReference>
    <dbReference type="GeneID" id="114147653"/>
    <dbReference type="KEGG" id="xco:114147653"/>
    <dbReference type="CTD" id="436857"/>
    <dbReference type="GeneTree" id="ENSGT00940000160306"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000004">
        <property type="entry name" value="Crystallin beta A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.20.10:FF:000002">
        <property type="entry name" value="Crystallin, beta B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.20.10">
        <property type="entry name" value="Crystallins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050252">
        <property type="entry name" value="Beta/Gamma-Crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001064">
        <property type="entry name" value="Beta/gamma_crystallin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011024">
        <property type="entry name" value="G_crystallin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818:SF7">
        <property type="entry name" value="BETA-CRYSTALLIN A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11818">
        <property type="entry name" value="BETA/GAMMA CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00030">
        <property type="entry name" value="Crystall"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01367">
        <property type="entry name" value="BGCRYSTALLIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00247">
        <property type="entry name" value="XTALbg"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49695">
        <property type="entry name" value="gamma-Crystallin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50915">
        <property type="entry name" value="CRYSTALLIN_BETA_GAMMA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="8">
        <location>
            <begin position="12"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="8">
        <location>
            <begin position="52"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="8">
        <location>
            <begin position="105"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Beta/gamma crystallin 'Greek key'" evidence="8">
        <location>
            <begin position="147"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000019237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="196" mass="23479" checksum="9A82F5D7B79F0E24" modified="2018-12-05" version="1">MNPQQMEQMGQFRITVWEEENFQGKRCEFMLECQNIMERGFNKIRSIKVENGPWVGYEYPEFQGQQFILEKGDYPRYEAWSGNSSYRTEHMLSFRPIKCANHSDSKVTLYECEDFQGRKFEMCDDYPSLQAMGWCSKEVPSMKVNSGAWVAYQFPGYRGYQYILERDRHQGEYRNYNEFSTQAHTNQVQSIRRIQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M8U6</accession>
    <name>A0A3B5M8U6_9TELE</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Early endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GGA protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5M8U6"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000016179"/>
    <dbReference type="Ensembl" id="ENSXCOT00000016385.1">
        <property type="protein sequence ID" value="ENSXCOP00000016179.1"/>
        <property type="gene ID" value="ENSXCOG00000012228.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156448"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034394">
        <property type="term" value="P:protein localization to cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14239">
        <property type="entry name" value="GAT_GGA1_GGA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1230:FF:000001">
        <property type="entry name" value="ADP-ribosylation factor-binding protein GGA1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000023">
        <property type="entry name" value="ADP-ribosylation factor-binding protein GGA3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000011">
        <property type="entry name" value="ADP-ribosylation factor-binding protein GGA3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008153">
        <property type="entry name" value="GAE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027422">
        <property type="entry name" value="GGA1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041198">
        <property type="entry name" value="GGA_N-GAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45905:SF4">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45905">
        <property type="entry name" value="GOLGI-LOCALIZED, GAMMA-ADAPTIN EAR CONTAINING, ARF BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18308">
        <property type="entry name" value="GGA_N-GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50180">
        <property type="entry name" value="GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="VHS" evidence="11">
        <location>
            <begin position="18"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="GAT" evidence="13">
        <location>
            <begin position="172"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="GAE" evidence="12">
        <location>
            <begin position="479"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="589"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000016179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="608" mass="66952" checksum="813D7AEA48AFA928" modified="2018-12-05" version="1">MAAAPSDETSLQPLINKATNPLNKETDWDSIKAFCDKLDNEPDGPQLATRLLAHKIQSPQEWEAMQALLVLETCMKNCGNKFHGEVGKFRFLNELIKVVSPKYLGSRAPELVKAKVLEMMYSWTVWYPDEAKIAEAYQMLKKQGIVKQDPVLPGDKPLPPPPPRAKTPIFEDEEKSKMLSRLLKSTHPDDLRAANELIKEMVQEDQKRVEKVSKRVNAIQEVKESVSLLSQLLDGYSKESYSHSNQELVRDLYQRCEKMRPTLFRLASDTEDNDEALADILEANDSLTQVINLYRQLVKGEEVSKDGTSLASQQGGSSALLDLTGLNISENTEPSNPQAAPQSLGLSLLDDELMSLGKQATDDLNLRVGINTLTNSSAPAVALQPAVVQTSQATPVGGCTATPRAMEELDLLGKTLLQQSLPPESQQVKWDKLQPHSKVPLRDLQAKSGASSSSSSSVDPNDRISLADVTVPLESIKPSSVLPVTVFDKHSLRVLFTFACDCPPSRPDVLVVIISMLSSAPIPVTNIRFQAAVPRVMKVKLQPPSCTDLPAFNPILPPAAITQILLLANPHKEKVRLRYKLTFNLGDKSHDESGDVDEFPPPDTWGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5M9D3</accession>
    <name>A0A3B5M9D3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Heparan-sulfate 6-O-sulfotransferase</fullName>
            <ecNumber evidence="9">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>alpha-D-glucosaminyl-[heparan sulfate](n) + 3'-phosphoadenylyl sulfate = 6-sulfo-alpha-D-glucosaminyl-[heparan sulfate](n) + adenosine 3',5'-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56604"/>
            <dbReference type="Rhea" id="RHEA-COMP:9830"/>
            <dbReference type="Rhea" id="RHEA-COMP:14621"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:58388"/>
            <dbReference type="ChEBI" id="CHEBI:140604"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the sulfotransferase 6 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5M9D3"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020883"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021140.1">
        <property type="protein sequence ID" value="ENSXCOP00000020883.1"/>
        <property type="gene ID" value="ENSXCOG00000015640.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183071"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017095">
        <property type="term" value="F:heparan sulfate 6-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001569">
        <property type="term" value="P:branching involved in blood vessel morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048048">
        <property type="term" value="P:embryonic eye morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001933">
        <property type="entry name" value="Heparan-sulfate 6-O-sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010635">
        <property type="entry name" value="Heparan_SO4-6-sulfoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12812">
        <property type="entry name" value="HEPARAN SULFATE 6-O-SULFOTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12812:SF6">
        <property type="entry name" value="HEPARAN-SULFATE 6-O-SULFOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03567">
        <property type="entry name" value="Sulfotransfer_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="216" mass="24440" checksum="EC2C612391F58DCA" modified="2018-12-05" version="1">MDEKSTSSHQRLLLFLLMVGIFGVFMIQYACPSSSECQMLHQLGAWFTLRGGPGIRIGDGESQDGLQKDPYIAEDGALARFVPRFNFTSADLNREVDFNIKGDDVIVFLHIQKTGGTTFGRHLVRNIQLERPCECHAGQKKCTCLRPGKKETWLFSRFSTGWSCGLHADWTELTHCVPSRMDSREAQKNQPRLEENHVKFTLLSNFYLPGLDVNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAH8</accession>
    <name>A0A3B5MAH8_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">trypsin</fullName>
            <ecNumber evidence="10">3.4.21.4</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.4" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAH8"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020565"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020815.1">
        <property type="protein sequence ID" value="ENSXCOP00000020565.1"/>
        <property type="gene ID" value="ENSXCOG00000008546.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244883"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000008">
        <property type="entry name" value="Cationic trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000005">
        <property type="entry name" value="Serine protease 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF15">
        <property type="entry name" value="RIKEN CDNA 2210010C04 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0222">Digestion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase S1" evidence="12">
        <location>
            <begin position="49"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="271" mass="29594" checksum="92A8F69776F1332E" modified="2018-12-05" version="1">MKYFILLALVAAACKFLPRIHLFLYYIKQGFKSLTLWFTDAAPLEDDKIIGGYECRKNSVPYMVSLNVGYHFCGGSLISSSWVVSAAHCYQSRINVRLGEHNIAVSEGTEQFIDSSTVIRHPSYNSYTLDNDIMMIKLNSPAGLNSYVNTVSLPSTCASAGTTCLISGWGNTLTSGTNYPDKLMCLDAPILSDTSCRNSYPGEITNNMFCAGFLEGGKDSCQGDSGGPVACNGELQGIVSWGYGCAQRDRPGVYTKVCNYNTWIRNTMSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MAM3</accession>
    <name>A0A3B5MAM3_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MAM3"/>
    <dbReference type="Ensembl" id="ENSXCOT00000020865.1">
        <property type="protein sequence ID" value="ENSXCOP00000020615.1"/>
        <property type="gene ID" value="ENSXCOG00000015444.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286959"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001639">
        <property type="entry name" value="Si:dkey-7i4.21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000744">
        <property type="entry name" value="zinc finger E-box-binding homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000097">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 208-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="185"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="213"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="241"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="297"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="77"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSXCOP00000020615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000261380"/>
        </source>
    </evidence>
    <sequence length="328" mass="37327" checksum="D2BB98486D9DA204" modified="2018-12-05" version="1">MLVSLSIYREGKILSPPCLVDVPRPQLSLSNQKRKSTLNEKELEDQQFREEENQLCISQDEEQLVLKQETGDILVTPSDVRKLNNETEPNRNQLVSQAFPEDENQVEEGSNFGDPGEKRDKEQKQNKRCQKTIRQKGTTEGPKQEKHKKSHLKKNLYPCTVCDKIFSGNESLMRHTRIHTGEKPFTCISCGKSFTLKSNLATHMRIHTGEKPFTCISCGKSFTQKSHLATHMRIHTGEKPFTCISCGKSFTQKSNLATHMRIHKGEKPFTCISCGKSFTRKSNLAIHMRIHTGEKPFTCISCGKNFTKKCSLTIHTRIHTGQHNIQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2018-12-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A3B5MB95</accession>
    <name>A0A3B5MB95_9TELE</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Sodium-coupled neutral amino acid symporter 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="30">Amino acid transporter A2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">Solute carrier family 38 member 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">System A amino acid transporter 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">System A transporter 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">System N amino acid transporter 2</fullName>
        </alternativeName>
    </protein>
    <organism evidence="34 35">
        <name type="scientific">Xiphophorus couchianus</name>
        <name type="common">Monterrey platyfish</name>
        <dbReference type="NCBI Taxonomy" id="32473"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Actinopterygii</taxon>
            <taxon>Neopterygii</taxon>
            <taxon>Teleostei</taxon>
            <taxon>Neoteleostei</taxon>
            <taxon>Acanthomorphata</taxon>
            <taxon>Ovalentaria</taxon>
            <taxon>Atherinomorphae</taxon>
            <taxon>Cyprinodontiformes</taxon>
            <taxon>Poeciliidae</taxon>
            <taxon>Poeciliinae</taxon>
            <taxon>Xiphophorus</taxon>
        </lineage>
    </organism>
    <reference evidence="34" key="1">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-alanine(in) + Na(+)(in) = L-alanine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29283"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:29285"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-asparagine(in) + Na(+)(in) = L-asparagine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:71383"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:71385"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-glutamine(in) + Na(+)(in) = L-glutamine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:68236"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:68238"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-histidine(in) + Na(+)(in) = L-histidine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:71583"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="23">
            <dbReference type="Rhea" id="RHEA:71585"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-leucine(in) + Na(+)(in) = L-leucine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29263"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:29265"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-methionine(in) + Na(+)(in) = L-methionine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:68240"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:68242"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-phenylalanine(in) + Na(+)(in) = L-phenylalanine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:68244"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:68246"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-proline(in) + Na(+)(in) = L-proline(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28967"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="22">
            <dbReference type="Rhea" id="RHEA:28969"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>L-serine(in) + Na(+)(in) = L-serine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29575"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="25">
            <dbReference type="Rhea" id="RHEA:29577"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-threonine(in) + Na(+)(in) = L-threonine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:69999"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:70001"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>glycine(in) + Na(+)(in) = glycine(out) + Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:68228"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="24">
            <dbReference type="Rhea" id="RHEA:68230"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A3B5MB95"/>
    <dbReference type="STRING" id="32473.ENSXCOP00000020862"/>
    <dbReference type="Ensembl" id="ENSXCOT00000021118.1">
        <property type="protein sequence ID" value="ENSXCOP00000020862.1"/>
        <property type="gene ID" value="ENSXCOG00000015620.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155486"/>
    <dbReference type="Proteomes" id="UP000261380">
        <property type="component" value="Unplaced"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015186">
        <property type="term" value="F:L-glutamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF207">
        <property type="entry name" value="SODIUM-COUPLED NEUTRAL AMINO ACID SYMPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 32" id="KW-0472">Membrane</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="14" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 32" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 32" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="17"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="33">
        <location>
            <begin position="135"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
 
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